LOCUS       DAZKCZ010000001        72616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000001 DAZKCZ010000000
VERSION     DAZKCZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.001"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..2329)
                     /locus_tag="ACG8KN_000001"
     CDS             complement(143..2329)
                     /locus_tag="ACG8KN_000001"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP9187101.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2547..3230
                     /locus_tag="ACG8KN_000002"
     CDS             2547..3230
                     /locus_tag="ACG8KN_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFP9187102.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3311..4030)
                     /locus_tag="ACG8KN_000003"
     CDS             complement(3311..4030)
                     /locus_tag="ACG8KN_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9187103.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4342..4548)
                     /locus_tag="ACG8KN_000004"
     CDS             complement(4342..4548)
                     /locus_tag="ACG8KN_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187104.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4793..5395
                     /locus_tag="ACG8KN_000005"
     CDS             4793..5395
                     /locus_tag="ACG8KN_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9187105.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5536..6816
                     /locus_tag="ACG8KN_000006"
     CDS             5536..6816
                     /locus_tag="ACG8KN_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9187106.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6829..7557
                     /locus_tag="ACG8KN_000007"
     CDS             6829..7557
                     /locus_tag="ACG8KN_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9187107.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7626..9149)
                     /locus_tag="ACG8KN_000008"
     CDS             complement(7626..9149)
                     /locus_tag="ACG8KN_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP9187108.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9401..10582
                     /locus_tag="ACG8KN_000009"
     CDS             9401..10582
                     /locus_tag="ACG8KN_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9187109.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10642..12270)
                     /gene="treC"
                     /locus_tag="ACG8KN_000010"
     CDS             complement(10642..12270)
                     /gene="treC"
                     /locus_tag="ACG8KN_000010"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9187110.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12721..15432)
                     /locus_tag="ACG8KN_000011"
     CDS             complement(12721..15432)
                     /locus_tag="ACG8KN_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFP9187111.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15442..16143)
                     /locus_tag="ACG8KN_000012"
     CDS             complement(15442..16143)
                     /locus_tag="ACG8KN_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187112.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16329..16928
                     /locus_tag="ACG8KN_000013"
     CDS             16329..16928
                     /locus_tag="ACG8KN_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9187113.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17009..18022
                     /locus_tag="ACG8KN_000014"
     CDS             17009..18022
                     /locus_tag="ACG8KN_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9187114.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18130..19047
                     /gene="rbsK"
                     /locus_tag="ACG8KN_000015"
     CDS             18130..19047
                     /gene="rbsK"
                     /locus_tag="ACG8KN_000015"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9187115.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19075..19470
                     /gene="rbsD"
                     /locus_tag="ACG8KN_000016"
     CDS             19075..19470
                     /gene="rbsD"
                     /locus_tag="ACG8KN_000016"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9187116.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19470..20843
                     /locus_tag="ACG8KN_000017"
     CDS             19470..20843
                     /locus_tag="ACG8KN_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9187117.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21200..23344
                     /locus_tag="ACG8KN_000018"
     CDS             21200..23344
                     /locus_tag="ACG8KN_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9187118.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23396..24436)
                     /locus_tag="ACG8KN_000019"
     CDS             complement(23396..24436)
                     /locus_tag="ACG8KN_000019"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9187119.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24713..26515)
                     /locus_tag="ACG8KN_000020"
     CDS             complement(24713..26515)
                     /locus_tag="ACG8KN_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9187120.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26529..27281)
                     /locus_tag="ACG8KN_000021"
     CDS             complement(26529..27281)
                     /locus_tag="ACG8KN_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187121.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27290..27652)
                     /locus_tag="ACG8KN_000022"
     CDS             complement(27290..27652)
                     /locus_tag="ACG8KN_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9187122.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27828..28700)
                     /locus_tag="ACG8KN_000023"
     CDS             complement(27828..28700)
                     /locus_tag="ACG8KN_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9187123.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28751..29239)
                     /locus_tag="ACG8KN_000024"
     CDS             complement(28751..29239)
                     /locus_tag="ACG8KN_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFP9187124.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29377..30168)
                     /locus_tag="ACG8KN_000025"
     CDS             complement(29377..30168)
                     /locus_tag="ACG8KN_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFP9187125.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30223..30831)
                     /locus_tag="ACG8KN_000026"
     CDS             complement(30223..30831)
                     /locus_tag="ACG8KN_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFP9187126.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30824..31141)
                     /locus_tag="ACG8KN_000027"
     CDS             complement(30824..31141)
                     /locus_tag="ACG8KN_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9187127.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31297..32025)
                     /locus_tag="ACG8KN_000028"
     CDS             complement(31297..32025)
                     /locus_tag="ACG8KN_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9187128.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32184..33065
                     /locus_tag="ACG8KN_000029"
     CDS             32184..33065
                     /locus_tag="ACG8KN_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9187129.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33302..34345)
                     /locus_tag="ACG8KN_000030"
     CDS             complement(33302..34345)
                     /locus_tag="ACG8KN_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9187130.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34348..35016)
                     /locus_tag="ACG8KN_000031"
     CDS             complement(34348..35016)
                     /locus_tag="ACG8KN_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9187131.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35158..37494
                     /locus_tag="ACG8KN_000032"
     CDS             35158..37494
                     /locus_tag="ACG8KN_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9187132.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37609..37920)
                     /locus_tag="ACG8KN_000033"
     CDS             complement(37609..37920)
                     /locus_tag="ACG8KN_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187133.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38101..39576
                     /locus_tag="ACG8KN_000034"
     CDS             38101..39576
                     /locus_tag="ACG8KN_000034"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFP9187134.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39623..40207
                     /locus_tag="ACG8KN_000035"
     CDS             39623..40207
                     /locus_tag="ACG8KN_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFP9187135.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40209..40628
                     /locus_tag="ACG8KN_000036"
     CDS             40209..40628
                     /locus_tag="ACG8KN_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFP9187136.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40909..41193)
                     /locus_tag="ACG8KN_000037"
     CDS             complement(40909..41193)
                     /locus_tag="ACG8KN_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFP9187137.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41545..42255)
                     /locus_tag="ACG8KN_000038"
     CDS             complement(41545..42255)
                     /locus_tag="ACG8KN_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFP9187138.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42252..43724)
                     /locus_tag="ACG8KN_000039"
     CDS             complement(42252..43724)
                     /locus_tag="ACG8KN_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9187139.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43862..44335
                     /locus_tag="ACG8KN_000040"
     CDS             43862..44335
                     /locus_tag="ACG8KN_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9187140.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44488..45039)
                     /locus_tag="ACG8KN_000041"
     CDS             complement(44488..45039)
                     /locus_tag="ACG8KN_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9187141.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45144..45674
                     /locus_tag="ACG8KN_000042"
     CDS             45144..45674
                     /locus_tag="ACG8KN_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFP9187142.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46157..46600)
                     /locus_tag="ACG8KN_000043"
     CDS             complement(46157..46600)
                     /locus_tag="ACG8KN_000043"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9187143.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46616..47776)
                     /locus_tag="ACG8KN_000044"
     CDS             complement(46616..47776)
                     /locus_tag="ACG8KN_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9187144.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48283..49983
                     /locus_tag="ACG8KN_000045"
     CDS             48283..49983
                     /locus_tag="ACG8KN_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187145.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50085..50918)
                     /locus_tag="ACG8KN_000046"
     CDS             complement(50085..50918)
                     /locus_tag="ACG8KN_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9187146.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51222..53387)
                     /locus_tag="ACG8KN_000047"
     CDS             complement(51222..53387)
                     /locus_tag="ACG8KN_000047"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFP9187147.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53647..55941
                     /locus_tag="ACG8KN_000048"
     CDS             53647..55941
                     /locus_tag="ACG8KN_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFP9187148.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55934..56599
                     /gene="pgmB"
                     /locus_tag="ACG8KN_000049"
     CDS             55934..56599
                     /gene="pgmB"
                     /locus_tag="ACG8KN_000049"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9187149.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56592..57599
                     /locus_tag="ACG8KN_000050"
     CDS             56592..57599
                     /locus_tag="ACG8KN_000050"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9187150.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57621..58640
                     /locus_tag="ACG8KN_000051"
     CDS             57621..58640
                     /locus_tag="ACG8KN_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9187151.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58921..60288)
                     /gene="nhaC"
                     /locus_tag="ACG8KN_000052"
     CDS             complement(58921..60288)
                     /gene="nhaC"
                     /locus_tag="ACG8KN_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9187152.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60397..61818)
                     /gene="tyrP"
                     /locus_tag="ACG8KN_000053"
     CDS             complement(60397..61818)
                     /gene="tyrP"
                     /locus_tag="ACG8KN_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFP9187153.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62061..63938)
                     /gene="tdc"
                     /locus_tag="ACG8KN_000054"
     CDS             complement(62061..63938)
                     /gene="tdc"
                     /locus_tag="ACG8KN_000054"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9187154.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64229..65485)
                     /gene="tyrS"
                     /locus_tag="ACG8KN_000055"
     CDS             complement(64229..65485)
                     /gene="tyrS"
                     /locus_tag="ACG8KN_000055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9187155.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65597..65802)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            66065..67366
                     /locus_tag="ACG8KN_000056"
     CDS             66065..67366
                     /locus_tag="ACG8KN_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9187156.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67435..68034)
                     /locus_tag="ACG8KN_000057"
     CDS             complement(67435..68034)
                     /locus_tag="ACG8KN_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9187157.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            68288..69169
                     /locus_tag="ACG8KN_000058"
     CDS             68288..69169
                     /locus_tag="ACG8KN_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9187158.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            69182..70792
                     /locus_tag="ACG8KN_000059"
     CDS             69182..70792
                     /locus_tag="ACG8KN_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9187159.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(70869..71528)
                     /locus_tag="ACG8KN_000060"
     CDS             complement(70869..71528)
                     /locus_tag="ACG8KN_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9187160.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(71811..72476)
                     /locus_tag="ACG8KN_000061"
     CDS             complement(71811..72476)
                     /locus_tag="ACG8KN_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFP9187161.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT        21653 a        14678 c        13005 g        23280 t
ORIGIN      
        1 acctcccttt tccgaataaa ctgtgttcca gaaatagctt cttcgaaaat aagctgaaat
       61 ttcacaaaaa tttgagaagc aattttcgta cattccttct tctttcttga aactgaccac
      121 ttctgtcaca ccctctcttc tatcactttc cttttttcac acgactcccg gtttctttga
      181 taaatctcga tactttcaat tttcttgaat ttttcatttg aggtacacat agatacagac
      241 gatttcttgc tctagtcatc gcaacataaa atagtcttcg ttcttcttct atcgtcgtta
      301 ctggttctgc ttttatttca ttaaagtcga ttactttctt ttctgtcgct ccgttataag
      361 gcaaacttcc ttcgttacaa ccaatcaaaa agacatttcg gtaactaaga cctttacttt
      421 tgtgaatgct ctggatacat agtgcatcaa gttttccttg tctgtaagca atcaagcggg
      481 tttttaattc tttagctgtc gcttgggttt cttgcgataa ggcttggagt cttctgaaag
      541 tagcatgctg ctttacttct tctcgtatcc attcttgatc actctggtcg atttccttag
      601 ctatacgtgg cagatgaaga tagctttgaa caagcgaata gatttcttga tgctggtctg
      661 ttttacgcaa cgcttcctca aactctcgga tttctgcaga tttcgatttt aaattggggc
      721 ttatcgacaa aatataatct gcaacggcta tattttgata tttaggagaa aagtattgat
      781 cgcattcact ttttttcagg tagcagtctg ccatcagctg ccggagcaat tgctggtagg
      841 cctgattttt cttttgcaga ttcagtgctg tttgccaatg aacaattttt tgatagaaat
      901 gttggaaaga cttgatacca aagatacctt catatggact atctgcttcc agtacaaaag
      961 gaatattcgt tttcagcaga gattggacaa gctgctgcgt ttgtgaagct gatcggtata
     1021 atatgattga ttccttatat ttcccatttt tttgaataaa tgtctgaatt tgtttgatta
     1081 gccactgact ttctgcttta gtatcagaaa acaaataggc ttccataaca cctgcttctt
     1141 tttgtgctgg aatcatcgcc tttggtatcc gatcattttt attatagctg atcaatctat
     1201 ttgctccttg gacaatagct gcccgagaac gataattagt taataagaaa agagttttgg
     1261 taggatattc tttttcaaag ttcttgatgt atgctggctc tgatcctctg aatccataga
     1321 tactctgatc atcatctcca acaacaatca acttgatcct gttttccttt gtcatccgtt
     1381 tcacgatttc ccattgaagt ggattagtat cttgaaattc atcaatgaat agataatcat
     1441 atttttggcc gatataatgc tggatctcct cattctccaa agcgcttttc agattttgga
     1501 gtacatcatt gaactctagc aattgctggc ttcttttttg ttcttggagt tccgtgagca
     1561 atccatacag tcgttctgat atcggctgtc gtgcattctt atcaatggaa tcatattttt
     1621 ctttaactaa ctgaaccatt tcttctactg aaaggttcat attctgccaa tagtctaacc
     1681 gagaagcaat cgttttttct ccatattgat caaatatata aagattatat ttacgtatca
     1741 gctgttgcaa aacagcattc actttttgtg cagtatcctt tgaaaagtct ccttgtattc
     1801 cttgcattgc tacttcagga aattttcgac aaatttcatt gtatatccta aaaaagatac
     1861 tatggaatgt tccgatatgg acagcggttt ttctctggct gaaattatct actcgttttt
     1921 ttagttcatt tgcagcgttc cttgtaaaag tgatcattag tatgcgttct tcgtctactt
     1981 gtttttgcgt catcagataa acagcttttg cacagattgt ttgcgttttg ccgctcccag
     2041 ctcctgctat gattcgaaga tgtttcttca tatcatatgt gatagctgct ttttgttctt
     2101 gattgaagcg attataggaa cgtttctcca tcattttaaa gaaagagctg tttccttcac
     2161 tttcttttac ttgtacttct tcctcaataa gtccgggtgc ttcgccataa gccgcttcat
     2221 aggtagcaat gatttcttct tgtagccgaa agacttgttg aagcttttta tacttgttct
     2281 cccagcgttt caattctttt tgtaatcgga gaatttcttt ttcattcaac tgattgattt
     2341 ccttcccatc tttcttcttt gaatttgttt atttcaagtt ggttatttaa agtttcgttg
     2401 tctatttttt ctttatacta acagaccttt ctaaaaatga aaatgggaag cgatatgatt
     2461 ttagttttct gaaaattttt cctttttttc aatcgtttat gctaagataa ggaagacaaa
     2521 aatattcata aaaggtgatg aaaaaaatgc agacttcctt tcacgagggg cgtacaatag
     2581 gacttttgct gacatttatc ggaggagcca tggacagtta tacttatatc caatatggtg
     2641 cttttgcctc tgctcaaact ggcaatatca ttctatcgat catccaagca tttgacggag
     2701 aatggctaag tgttggtaaa aaagtattgt ctaccctttt ctttttctta gggattcttc
     2761 taacaaagta tctaattgat tattttagaa aaaaagaaaa acatttctgg cgcttgtttg
     2821 ttctatatta tgaagcagtg atttttttct ttgttagttt gacacccgtc catgagcatc
     2881 ccgcattagt aacgattttg attgctttta ctgcatcgat ccagtgggta gcttttgata
     2941 aaatcaacgg gcgtgcctac actaatttat ttacaactgg aaatttaaaa ggggtagcaa
     3001 cgaacttata tgattattgt atttctaaag aaaaagcaga ttttgatcga tttttccact
     3061 atctaagtgt cgttcttgcc tttatcgcag gagctattgt ccttgttttc tgtcatcatc
     3121 tgtttggcgc ccattcgatt ttgatcgttt ccattttatt tttgatcatg gccctcactc
     3181 agacatttca tttatggcaa ttttatcata cgaatcggtt cactatttga tctcaatgaa
     3241 tcaaaaaaac taagcaagat gctgctcaca gagaaatatc tgcgagtgag caatttgctt
     3301 agtttttata ttatttagtt gtaaagattc cctttgcttt cgggttattt gtagtgtagc
     3361 ggatcgactg ggcatctgcc tcttctgtca ttttctttgc ccgcatcttg atttcttgta
     3421 cgatttgttc tccacatgcc ggatcggcta cgaaatcata tttgtcccca tcgatcacta
     3481 ttttgggaca tatatcaaac tgttcatacc actcttcgta tcgtcgattg agttctttat
     3541 agtaggaata gagttcagga tcttgttcta gctgttcata ttcccggcct ctttttttga
     3601 ttctcgctaa catagtttca aaggatacac gaatataaac cagtagatct ggtttctttt
     3661 gtggcgaaat ttcttctaac tccttcagca tcgtttctaa aagaccttca tactgcttta
     3721 cttctacttc agaaacacgc cctaaatctg cattcaaatg aaacagcata ctatcttcgt
     3781 agatggagcg gtctagtacg ttgtcatcat ggctgaaagc atctttgatt gccaaaaagc
     3841 gtttgttcaa aaagaaaatc tgtaataaaa aagtatactt ttctggatta gaataaaata
     3901 gcggcaatac ttcattatct tccacgttct cataaaaagg ttttgtttct aaatcttggg
     3961 caagcatttc agttagtgtg cttttccctg caccgatcgt tcctgcaaca atgatcacaa
     4021 ttatcggcat ccttcctgta ataaaatctg ttcttcattt tactacttca tttatagaaa
     4081 taaaagcctt gactttatct gctccttaaa agctacttct ctaaaagtag ccaaaagtaa
     4141 ctcctattca tgaaaataaa ggctgtgaca aaagtcttgt cgcagccctt ttttcaaata
     4201 aacagtattc tcctgccaac acctcggtca caaggcaata ttcataaaac atgaaaagct
     4261 gttttttaac attattcctt gtaactcaga gctaaacggg agactcacaa cctctttatg
     4321 ctctcaacat tttatctgtt tttattcttc tattgtactt gatttgcgta caactaactg
     4381 ccagtgcaga ttatgagcgg tatcattgtc ttctactgcg catttcatga cacgtatcat
     4441 cacactattt tcatagattt tttctatttt tccttttatt ggtgattcaa ataaatcaga
     4501 tttaaatgca tatgtttcgc cgacttcaaa caattgttca ttttccataa gaatccctcc
     4561 atcacctttt ataaggtcag taatttttta acataaatca ctcgaaaaag cacaaacttg
     4621 tcataaaaag tacttttcgt atcaaaactg tcactttttt tggtaaggtt cttggaaaac
     4681 gaagattttt ttaggagatc atgacggttt tgatgtcaaa accacttttt atgaaacaaa
     4741 agacgttttt tatttttttt agcataaaat ttgtggtgga ggtacgtatg agatgaaaga
     4801 taaagttgtg aacaaattat tagaagcctt attcagcgga gacaaatcgg aagaagatat
     4861 ggtgtatgtc caagtaaaat ttgtcttaga agttttattg aataatcttg gaaaactagc
     4921 ggtcgttcta gtcttttctc tggtgacagg ctcatgggcg gaaacaggaa tcacctttct
     4981 ttcttatatc tgcatccgca gatatgctta tggattgcat tccgactctg aattcgtttg
     5041 tctgctgtgg acactacttt atttatgggg cgttcccttg gtgatgaaac atctgcaatt
     5101 gactatctcc ttcccaatga tggtcttttt gttaatcagc tgctttttgt tactgcttcg
     5161 ctatggatca agaggtacag caatcaatcc cattgaaccc gagaaacgtc ccccattatt
     5221 aaagaaggcg atcagcatgt ttctaatttt ttctctcatt acgttatttt tctcagcttc
     5281 ttacttttct acctatttgc tacttgggat tgttttagaa atagctacgt tgcttcctat
     5341 tacaaactac ttgatgaatt ttggaggaat ctttcatgaa aaaaacaatc attaaacaat
     5401 taactaactt atctgacttt tttgtatctg gctgctgctt tggtatgagt tctgaattca
     5461 aaatccctga aaaattaaaa aaataatacg ccaattgtgc tataatttgg taaaaatcaa
     5521 tgggagctgt actttatgtt tttatggtcc atagcgttgc aatatttgat ttacacaatt
     5581 tgtatcttat tgattgacca tcacatccga tacaaatatc tgtttgcggg agtcattctg
     5641 atttcttgta ttttgattgg atgggtctat tcgattttag ggatttttac tgctctaatt
     5701 gctataggaa tgctgatgct tacttttttc ttcttttcga cttcgcgtca acaattggtt
     5761 tacgcgcttc cttttgggtt attgacgtcg cttttaggag atcacttaac aagcatcggc
     5821 gatttttttg ttttgaaatc ttcggttatt gaaccaggag attcattgca gtattttcac
     5881 attgtcttat ccgctttttt atctgcttta ttagcttttt gtttaaaaaa atcgttagag
     5941 acttggtttc acactgtgga ccgccgtatg atcggtacga tcgggacatt gatattattt
     6001 acatactata tcattatttt ttatacccgt ttttctggtg aaacacctga tgtcttagct
     6061 ttgaatacga gttttttcat tctatatctg tgtgttggcc tgatcatctt ggtttattat
     6121 tggaaaatct ctaataagaa attagctgat cagttattag agcaacaagt caggatgcaa
     6181 caggattata tcaaagatct agaaaaaaac tatcaagact tgagagaatt caaacatgat
     6241 taccaaaatc tgcttttttc tcttcacagc tatatttcag aaggcgactt agaaggatta
     6301 cggtcttatt atgatcaaac gatattacct acacgccaga tgatgaacct ttttccggcc
     6361 aatctaagta tgttggataa tatgaaagtt cctgagatcc gtagtttact gtcattaaaa
     6421 ctcatgatgg cacaggaaaa agggttgatc gtccagcttc attttcctga gccaatcgaa
     6481 ctggatacga aacatacggt aaaccttgta cgtatgcttg gtattatctt ggacaatgcg
     6541 attgaaggag ctagtgctgc aaagaagaaa aaaatcgaat tgaccatact caagaagaat
     6601 cattccgtga tgattcgtgt gatcaataca acaacaaata cattaccgtt aaaccagttg
     6661 aaacaaaaag ggttctcaac aaaaacaaat ccaaaaaacg aaggccttgg cttgtttatc
     6721 ttggatgaat tgacaaagac caatccttat cttttccttg aaacatcaat tgaaaaccag
     6781 cgtttttctc aaacgattta tatacaaata aaataaagga gtaatgatat gattcccatt
     6841 tatatttgtg aagacgatcc aaaacaatta gaagttttat caacggtgat caaaaataga
     6901 atcatgattg aaaacttaga cagttatgtc catatttcta cctctgatcc ggaagaactt
     6961 ctcaatgcag ccaagatcag aacttcttct tttggtattt actttttaga tatcgaacta
     7021 gaagacagtg aaatggatgg tgtagcgatc ggtaaattga tccgtgaatt agatccttta
     7081 ggaaaaataa tctatgtgac ttcccatacg gaaatgtcga tgaaaatctt aaaaagcaac
     7141 atcgaaccga ctgattatat cgtcaaagaa gatctttatg atttaaaaga aaatgtcgag
     7201 catatcctga gcaaaatttt cgaagatttt caaatgtccc aaatccaaaa agatatcttc
     7261 aaaatagagt ttaatgatga gatcaaattt ttaccgatca aagacatcca atatttttct
     7321 acttcgctag gagcgccaca taaactggaa gtccatttga ctcatgccca attacaattc
     7381 tatgagaaga tcaaagaaat cgagaaaatg catcggaatt ttatccgttg tcataaatcc
     7441 tatgttatca atacacaaaa tgtgcggtcg attaataaaa aaaccagaga agtcacgttg
     7501 gcaaacggcg agaccatccc agcctctatc cgtggattaa agaaattgat ttcataaaaa
     7561 gaaccgaata gtttggaccg actaaatgac cagccgatcc aaacccttcg gttctttttt
     7621 gcctatcagt gcaaaagcat ttcctgtgca atttcacgtc catccattgt atctggataa
     7681 aatgttggcc aattctccat ttctttcagc aatacttctt gactttcatt tcctgcatat
     7741 aaatgaaaat gttcggagtc agccggtgca atcgtgtgat cactgaattg aatatatcta
     7801 aaagcgccac tttccttatc ttctgcttcg aatagatacc ttacgccacg gtttcctgcc
     7861 tcgtaggtca agatttcttt ccctacataa cgataatttg aacgtttcgt tgttccattc
     7921 ttaaacgtaa atgccatctc tttctcactg atcttgattg aagcaatatc tgtttgatag
     7981 cccttcgtat aatattcttt atattcttgt gctgtcttat cctgattcaa tttttcttta
     8041 tattcaaaaa caggatcaaa agtcccatct tctacatacg tatctactgt ttgccaagtt
     8101 cctgcccaat ctgaaagaga gcgatcttgt acatcctcgt cttcaaaata accgttgtag
     8161 acgctctttt cttttttggg ggtcaaagta tcttctagcg ggttttctgt ctctgtcgtt
     8221 tttttcaacg cttcgaggtt tgcttccatg atagaaagat agttttctcc attttctatt
     8281 tgttcattcg tcaatccttc caaaggattc aatacttcta aagatacacc tgcttcgttt
     8341 gctaacgttc tggcaatact atctttggcg ttttcttcaa aataaatata gttgattcca
     8401 tgttctttaa caaagtgttt cagttcagca attctcgccg cagacggttc ttcactagaa
     8461 gaaagaccag caatcgatac ttgattcaat ccatattcta acgctagata gttgaatgca
     8521 gcatgttgcg tgacaaaaga tctttgtttt gcttgcttaa aggtttcttg ataaagctga
     8581 tggagtgctt gaagtttctc ggtataggct gctgcatttt ttgtccatac ttcctgtttt
     8641 tctgggtaag cttcaatcag ctgatcacgt atgtttgcta cctgcttgat tgctaaactt
     8701 ggagctagcc atacatgtgg gtctagctca tgtgtatggc cttcttcccc gtgttcatgg
     8761 tcatgatcat gtccttcttc acttcccgga agtaaaagca taccttctgt cgcttttatc
     8821 gtatggacat tgccttcttg taatgttttt tggattgcgg ggacccatgt ttccatgttt
     8881 tcatcattat aaacaaagat atctgtatct tggatctttg ccatgtcttt agcagaaggt
     8941 tcataatcgt gggaatcagt ccctgctgga atcaataact ctacttctcc ttcatccccg
     9001 acgattgctt ttgtaaaatc atacatggga taaaaggtcg tcatcacgct caatttttct
     9061 ttactgtcct cggctttccc acttgccccg caagcagtta acatacctgc taaaaccagc
     9121 atggccaata tacctatcaa tcgtttcatt tatgacactc ctcttctcat cttctttcat
     9181 ttgaatgttc cgattcgctt ttcgcaaacc gtaatagtta cgttttagca acaggattta
     9241 atttatcata tggttaaaag aactgtcaag cgttttctta ttttaattga tgagaaagtt
     9301 tatatgaatg attgacgata tttttgcaat caacctctat cattttcctt ttatacccgc
     9361 ttttgtctat aatgaagtta tagataaagg aggaaaaaga gtgaaaaaag aaacagatac
     9421 aacgaatcgt ttgatccgct ttttaggagg aaaaacctct tactatgttt taggaatagt
     9481 tatcttactt gcgctgatca tttatttttt ccatcagatc tcgtttatat tcaagcctgt
     9541 cggaatcatt atcgctacta tcttgccccc attattgttt gcactgatct tgtattatct
     9601 tttggaacca gtcatcaaac gtttgagcaa gcacctttcg aggaacttgt cagtcattat
     9661 cgtttatgtg attattttat ttttattggg gcttggaagc atatggctta ttccttttct
     9721 tgaagaacag gcaaagacat tgatcagtag tttgccagat ttgtttcaag attttcaaga
     9781 gagtctccga caatttttgg agaaaacacc ttttgccgat tcatttaatc aatttttcgc
     9841 ttctattgac gacgtgacgt ctgatatagc tggatttatt ggaaattatt gggaaagtgg
     9901 cgcacaacgg atcagcaaca ttttttcaac tgttacagct atttttatta ctttatttac
     9961 tggtccaatt gtcgcttttt tccttttgag agatcctcaa aaattttatc agtcagtttt
    10021 agcgattgtc ccaccagcat ttcgtacaga ttttaaaaat ttgacaaaga ttgctaatca
    10081 acaattgggt tcatttctaa aggggcaaat cattgcttca tttatccttg gagcagtcta
    10141 ctgggtttgt ttcttactga tcggattaga atttgctagt gttttagcta tttctgctgg
    10201 gttgttatgt atcattccat atattgggcc gtttatcgcc ttctttccag gtttgatcat
    10261 tgcattccaa gactctactt ttatggctgt caaatttgtc attgtctggt ttgccgtaca
    10321 acttctccac ggtgacttgg tgattcctcg agtaatgggt gatcgtttac agatccatcc
    10381 gattacgatc ttaatcgttt tacttgtcat gggtgatcta atgggaattg tgggcgttat
    10441 ttttggtatt cctatctata cgttagtcaa attactcgtt acttttattt tccgtaaatt
    10501 caaacaacgc tacaataaat tttatggcga taaaggagag tatgaaaact cttcattttc
    10561 tgaagaagat tattttaaat aaaacacaaa acgtcgatct tacgagaata gctcgtagga
    10621 acgacgtttt gtgtttttgt tttaattttt cagcaaagca attgcctggt atggttttag
    10681 ctccagactt tgatgaagaa cagtatcgga ataattatcg ataagaatat tggcttctgt
    10741 gaatccttct gggatggcga tccttgtatc tttatcatag aaatttgtta atacaagcag
    10801 ctgctgtcct tcaaattggc gaagaaagcc ataaacttcc ggatgatttg cttcataagc
    10861 atgataatct ccttgtgcaa cgattggata tgtttttctc agccgaatca atttcttgta
    10921 ataggacaaa atagaatctt cagaagatag ttctttttct acattaatct cttctagctt
    10981 gcctccaact tttaaccaag gtttccctgt agtaaatcct gcttgttctg tgtctgacca
    11041 ttgcatgggt gttcgagagt tatcgcgaga ttttgctttg atccgacgaa acgcttcttt
    11101 ttctgtgaat ccttgttcaa ggagtaactt ataagcattc aaactttcta cgtcaatata
    11161 gtcggaaatc gatgaatagt ctgggtcgat catgccgatt tcttctccca tataaatata
    11221 gggcgtccca cggttcaaat ggatcatggt tgctagcatc tctgctcctt gttttcgata
    11281 ctgatcgata gctacaaatc gattgagcgc acgtggctga tcatgattat taaaaaatag
    11341 agcattccag ccgttaccct cactcatctt ttctccccat gtatgaaata gtcgctttaa
    11401 ttctgcaaaa tcaaatggag gagtagacca tttattccca ttttcataat cgactttcaa
    11461 atgatggaaa ttaaagacca ttgataattc ttctctacta ggttctgtat acaagataca
    11521 attttcgatc gtcgtagagc tcatttcacc gactgtgatg atagtggaat ccgtcccaaa
    11581 cgttgcttgg ttcaattctt ttagatacgt atgggtgatt ggtttgtccg tatattcagg
    11641 tttgccatca ttttctttat tatttttcaa ttgttcgtct ttaccgataa cattgataac
    11701 atcgaaacgg aatcctttta cccctttttc catccaaaaa cgaacaactt tgaatagttc
    11761 ctctcttact gcttggttcc gccagttcaa gtcagcttgt gtccgatcat ataagtgcaa
    11821 ataatatttc cccgtatctc caaatggcga ccaagcactt ccaccaaatt ttgataccca
    11881 atccgttggc tcttctctta aaatgaagaa gtcctgataa tgctggtccc ctgctagtgc
    11941 ttttttgaac cattcatgtt ctgtcgaaac gtgattcagc accatatcga gcatgatttc
    12001 tatccggtat ttctttgctt cggctattag ctgttcgaat tcctccatcg taccaaagat
    12061 aggatcgatg gctgtgtaat cagagatatc ataaccgttg tctttttgag ggcttggata
    12121 aatagggttt agccaaatca tgtcgatgcc tagatcattc agatacggga gtttttcttt
    12181 gattcctttt aaatctccaa ttccatcacc attactgtct ttgtaagatt tgggatagat
    12241 ttggtaaatg actttgtctt taaatgtcat ggatatttac ctccattcaa ctgatttatt
    12301 acctaccaca acttatatat acaagttatc atttattatt ataactgaac cgaaagcgtt
    12361 tgtaaaattt atctttatac attctaagag gtctgttgcc caataaaaaa caagagatca
    12421 gacatgtatt tgtctatcct cttgtttaaa atagcaaaat attcttgttt cttctataaa
    12481 tggtgttcaa aagcttttat ctacggcaat cagtcttctc aaatgtaaaa catgaggagc
    12541 taattttatt tgagtattct ttttgctcga tgcaggacgg cttttttaca acctctgatt
    12601 catggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa tatgaaaaac
    12661 tattttgccg tttgtttctc taatactcag gtcaaaccgc tgatttcaca acctctgttt
    12721 ttattcattc gatttcaacg catcaatcat atcgatatgc tttaatttga aatgggtaac
    12781 aatcatcaca ataaccgtaa atacgatagt taatcctgct gataagacat aagcccacca
    12841 tgtgatcact aatggaaaga tgacattctc catagacgct tgttgcaaga tgaagtcagt
    12901 caatatatat cctacaccaa atcctccgat gattcctaaa agagtaaaga tgatattttc
    12961 tcgaacaatg tacatcgtga cttctttatc aaaaaatcca aggactttta ttgtagataa
    13021 ttcgcgtaca cgctcggaaa tattgatatt agttagatta tacaagacga taaaagccag
    13081 cagtcctgat aatacgacaa agattaagac aaccgagtct aagttcgcca ttgattcttc
    13141 ttgcgtttcg atttgtttgg acatgaaaga cgtatttgtc acagcgtctg tggcaagcag
    13201 tttctcggaa agcatttctt cttcttttgt tgtcatctta tctgtacgca gcaaaaatgc
    13261 attatccatc atcgattgat cagtaactga agcataatat tcaggggaca tataaataaa
    13321 gctacctaga tagtttttca cgatttcttt tacttcgact ttccattctt tttgatcttc
    13381 atcataaagc gaaatatgat ctcctgtaga taagtcaaat agttctgcta ctttttgcgt
    13441 aatagcgatt cctgttttag gcaaggagcg tgggttctct tcaagatcag ttaaatggat
    13501 aaaacgatcc aaatcagctg ttttacttgg tacgatcact gtcagacttt gcgaagcatg
    13561 tccttttttt ctcaattcta cggtttgagt atgaatagct aggatctcat ttactttttc
    13621 ttgcttctgc agcatatccg ctgctttttg acgactatct tccgtggatg aatccaatgt
    13681 aacgattgct tgatagtcaa tgatcggtcc aaattgtttg gctgaaacag aacttagaga
    13741 atctttcaat cccacgcctg caaccatcag cccggcacat ccagcaattc cgataatcgc
    13801 cataagcatt cttgacttat atcggaagag atttcggtaa ctgatttttt gattaaagct
    13861 aaggcgtgac cacagcggag tgatgtattc aagaaaaatg cgtttccctg gcttcggtgc
    13921 tttcggttga agcaatgcag caggccgttc tcttaaatct ttgaataaaa cgatcattgc
    13981 agcgccaaat gcggcaatga agaatgcggc agctgcttgt aaaatcggcg gccagtcata
    14041 aaaaataagc gcactcccaa tatcataccg ctcgttagac agctcaaaaa tgatccttgg
    14101 caaaagattc gttcctaata tcgttcctaa aacaatacct atccctgagg ctagacttgc
    14161 atagattgca tatttttttg cgatttcaac tttcgtataa cctaacgcct ttaatgtccc
    14221 gatttccctg cggttttctt ctaccatacg tgtcattgtc gtaaaggtga tcaatgcggc
    14281 aataaagaaa aagaaaacag gaaaaacatt ggcaatcgca gcgatacgat cagacagatc
    14341 accaaattct tgaaaaccga cattttctga acgttcattg tataggtatg atggtttagt
    14401 taaattttca attttttctg catttcgatt caattcttcc tctgcagagg ccagttcacg
    14461 ttctttctca actaattgcc tttcttgttc agcaagttga gcttcttgtt gagataatgt
    14521 ttgaacagcg attgttcgtt gcgcttcagg aagctgctgg atagttgctg actgttgcgc
    14581 taactgtttt ttagcatctt ctaaccgtgt tcttgcttgc gttgtttgtg ctttgccatc
    14641 cgctattttt tgctgtgccg gttctaattc ttcgttggcc gattttttca gctcttctaa
    14701 gcgttcttcg ggtcgatctt gtaaatactt ttctgccttt tcttgatttt gctccatttt
    14761 atctttgtaa gctttgctgt acgtattcaa attctttacg tttgtaaaat caagatataa
    14821 gacagaataa atatctgttt taaaaacgga ttcaggtaaa tagacaaaat aatcgacgct
    14881 accatttccg acgttcgcgt atcctctctc caaattgttg atgaacaatg gagaacggac
    14941 aaagcccgtg atggtatact ctttttcttt caattcatct gaatcaatct gataaatatc
    15001 accgatttta tacccttcat cttccgcttt ttcatccaat acaagctggt ttggtttttc
    15061 cgggagtttt ccatcagtta attgtagctt gttctgcttg ttatttttgt tataagaaaa
    15121 tatttgaatc acttcgtttt tgccagtatt ggaataatag atctgttttc ctgtttctac
    15181 ttttgcatct gggatctttt ctgcttgagc aatatctttt tgtgtcaagc caccagtaga
    15241 aacgatttgc aaatcagaaa gtcctgctgt accaacatag tgatccatcg ttttttgcat
    15301 gacaggtcca gcagttttga cacctacaaa taaaagtgtt cctagcatga caataaggat
    15361 gattgcaatg aatcgtcctt tagattgccg aatttcgcga aaacctgctt taagatatgt
    15421 tcttgttttc ataaaatccc cctaccatac aatctcatca atggatgaag gatgatcatt
    15481 taattcaaca gaccttactt tagcatcatt aatgtgaatg actctatcag cgatgggcgc
    15541 taaagcagaa ttatgtgtaa tgatcaaaac ggtattgccg tgttgtctgc tagcgttttg
    15601 caataatttc aatacttgct tgcccgtttc aaaatccagc gcgcctgtcg gttcatcaca
    15661 taaaagaagt ttgggatttt tagctagtgc acgagcaatt gaaaccctct gctgttcgcc
    15721 tcctgataat tgggacggaa agttattcaa tcgatgagct aggcccactt gcctcaatac
    15781 ctctacagga tcaagcgcat caggagaaac ttccgttgcc aattccacat tttcttttgc
    15841 tgttagatta ggaacgagat tgtagaattg gaaaacgaac cctacatctg ttcgtcggta
    15901 agcagtaagc tgtttatctg aaaattgcgc aatatctgtg tcatcaataa tgatctgacc
    15961 ttcatcaggt gtatccatcc cacctaaaat attaagaatc gttgactttc ctgctccact
    16021 tggaccaagt ataatcacta attcgccttt ttcaattgaa aaagaaatat cgtcatttgc
    16081 tgtaatggtc gtttctccca ttttgtatcg tttgtattca ttttttactt cgacatactt
    16141 cactctgatt cctccatttt cacgatattt ctcgctatat ctatgcatcg aatgcgtttt
    16201 ctcacactta tttattttac cactgaattt ctctagacta aagaaaggaa tcctttaatt
    16261 tagttgacaa ataaacgatt tatgatttac ttacataaaa aacattgtta tttgaaagaa
    16321 ggaacatcat gaatgattta aaggaaaata tgatttttga tttatcaaaa actgcttttg
    16381 tcgcgatcga catgcaaaaa gagatcgtaa ataacggtac actctctcca cacactgcag
    16441 catctattct gacaaacaat gatttgttag taagaacatt gaaacgtacc gctgccttga
    16501 aagttttagt gaacgtagac atctccactt tcccttattt gagtcaacaa actgatatgg
    16561 gtggaatgac tgcttctgta cctccagaat tcactgattt acttttgaag gattctttga
    16621 aagatacaga taatatgttg actatcacaa aatacaatcc aagtgctttt tttggtactt
    16681 ctcttgatct tcaacttcgc cggcgtggaa tcgaaactat cattttaagc ggtgtagcga
    16741 caacaaacgg tgtatatgct actgcattag atgcctttca acacgggtac catatcgtgc
    16801 ttgctgaaga tgcttgcagt gatcgtgaca aagaatccca tcaattgttc ataaaaaaga
    16861 tatttccgaa aactgcacga gtacgctcaa cgaaacaaat aatcgaagct atccaacaaa
    16921 gcaaataagt actggcacac gtgattttca tttacttctt tttgatttca tgggaaaata
    16981 accacaggat ttagatagga gtatgaacat ggtgaaaaaa aagatcacaa tcaaagaagt
    17041 cgctaaactt tccggcgttt caatcacaac tgtctccttg atattaaatg gaaatacgga
    17101 gaaattcagt ccgaaaactg ttcagaaagt tttagctgcc aaagaagaat taggctatca
    17161 gcctgactat tttgctagac aaatgattac caaggaaacg aaaacaattg gtgtactcgt
    17221 tcctgatatc acgaatccat tcttcagtac actgatgcga ggaattgagg atatcttgta
    17281 taagcagaat ttcgtcacga tattatgtaa tgctgattct gatcatcaaa aagaaatcga
    17341 atatctagcc gagctcacac gtcgaggtgt ggatggattc attatcgcga cttctgctgt
    17401 ctctacagat gcaatcaacg aaaatttgaa aaaacaaggc cggcccttca ttgttttgga
    17461 tcagaaaaaa tctgaaggat ttagtgatgc cgtacgaaca gacgattttc gcggtggtta
    17521 cctagcgggc atgcacttgc ttagtctagg acatcaaacg atcgcccttg tctatcctga
    17581 aaatccaccg gaaaatgtcc acgctcggat tgaggggttt aaatctgctc ttgatgttta
    17641 ccaaatccct cacgaccaac tgatcctctt acctacacag ttctcaaaac aaggtggcta
    17701 ccagatcaca gcagaacttc ttgattctgc tgcaactggt gtgtttgctt taaatgatga
    17761 attagctttt ggattatatc gcggattaga ggaagctgga aaaagcatcc ctgaagacta
    17821 cagcattatt ggctatgata atatagatat gtgcgagtat atcaagccga aactgacaac
    17881 gatcgcacaa ccaatcttcg aattgggaca aacatctgcc aagttattgt tagatcggat
    17941 ccaattccca gaaaaagaat gggaagaaaa aagattacct gtacgattag aaaaaagatt
    18001 ttcaactgca cccttgaaat agctttcaag gtgtgctata tttttatcga actattaaaa
    18061 cgttttacta aaacgtttta aaaaataatt ttcccatcaa aataaatgag atagttggag
    18121 gtttgtaaaa tgaacacggt tacagtaatt ggaagtatta atatggacac tacccttcgt
    18181 ttaaccaaca tgcccaagcc aggcgaaacg atgcatgcgc atgagatttt tcacgctggc
    18241 ggaggtaaag gtgccaacca agcagtggca gccaaacgtt caggcgcacg gacatctttt
    18301 attggaggcg ttggggctga cagcgaagga caacagttat tagatctact gacaaaagaa
    18361 aacatcgata catctgggat agctgaaatc caaggcgcca caaccggaca agcaatgatt
    18421 atggtagacg cagcaggaga aaatagtatc ttgatccatg caggggcaaa taatgctttc
    18481 catgaacaag aagtcctaaa aaacaagcaa ctgattacaa acagtgactt tattattgct
    18541 cagtttgaaa gtagcttaga tgcgacgatc ctagcttttt ctattgcaaa agacgcagga
    18601 aaaacaacga tcctgaatcc tgcaccagct cgtgaaacga ttcctactga actacttgaa
    18661 aagacagata tcattatacc aaatgaaaca gaaacagaaa tcattacagg aatcagagtg
    18721 acagatcata acagtttggt cgcagcagcc gaaaaactcc atgaattagg tattggaaca
    18781 gtaatcatta cactaggcag tgccggcgct ttctatcata cagaaaaaga acacggtatc
    18841 gtccctgctt ttaaagtgga tgccgttgac acaacagccg caggtgatac atttattggc
    18901 gcattaagca gcacacttca gccagacctt agtaacttga aagaagcgat cctttatgga
    18961 aatctagcct cttctgttgc tgtgcagtct tatggtgcgc aaccttctat cccttaccgc
    19021 gaagctttag aacaatcagc agtctaaaat taacaatgag aggaagaaac aaacatgaaa
    19081 aaaggaaaag taatcaattc agatatttca cgagtaatcg cacaaatggg gcactttgat
    19141 aaattaagta tcggggatgc aggcatgcct gtcccctttg gtactgaaaa aatagatttg
    19201 gctgtctcaa acggtcttcc aagtttttta tctgttttag aaaacgtttt agaagaactt
    19261 gaggtacaat cgattgaatt ggcggaagaa ataaagacaa tgaacccgga aatattggaa
    19321 aaaatcaaat tattgttacc agatacaccg attagcttta tcccacatga agaaatgaaa
    19381 aaagagttga ataactgtca tgcatttatt cgtacaggcg aaatgacacc ttacgccaat
    19441 attatattag tgagtggagt ggtcttctaa tggaaacgac aatgaatcct gaacagatgg
    19501 tactgtccga gcagattcat atgctgattg aacagttggg aggaaaagac aatattgatt
    19561 ctgtccttcc atgtgcgact cggcttcgta tcaaattgaa aaaacagcca gtagccaatg
    19621 cagaaaaatt aaaacagcaa atcccttcca ttaagtattt cattttactc gatagatatg
    19681 cagaaataat catggatcag ggattggctc ctgcttacaa ggaattgacg cgtcaaatcg
    19741 atcattcagc tccgaaagaa aagctaaatc cgatcgaaaa actatttggt ttgatatcgg
    19801 aaatctttac gccgatcatt ccgctgtttg ccggttctgg tatattaaaa ggtctggttg
    19861 ttttagcgac tacgattggc tggcttagtg aaacaagcgg aacttatcat attttgtctg
    19921 ctgcgtcgaa tgccgtcttt tattttctgc caatctttct tgctttttct gctgccaaaa
    19981 cgtttaaagt gaatggatat attgcggctg tcattgctgc agcactgatt gaaccgaatc
    20041 tgacctcttt actttcagac agtggcaaag cagctactga ttttctgggg attcctgtag
    20101 tactgatgca gtatacgtct actgtcatgc cggcgatttt aggtattttc ttttattctt
    20161 ttgttgaaaa attcctcaaa aaatatattc gcgaaaatat gcaattagta tttgtacctt
    20221 tactttcatt agttatcacg gttcctttga cgttaatggt attcgggcct gcgggtgttt
    20281 accttggcga aggtttagct gccgcaatta cttggatgat ggatattaac ggaccgatca
    20341 gtggcgcaat catcgctggt ggttggaata ttttggtaat atttggtatc caatgggctg
    20401 taaaccctgt catgatcagt aatatctctg cttatgggtt cgaccgaatc gttccgttga
    20461 caggagcagc caattttgga atggctggtg cagctttagg tgtatttctg cgaagtaaac
    20521 gttcaaaaac ccgttcgatc tccggttctg cttttgcttc gattttactt gcgggagtga
    20581 ccgaaccaac tgtttacgga attgcgatcc cattgaaaaa accatttgtt gctgcttgca
    20641 ttggtgcagc tgctggtgga gctgtaatgg gatttgccca agtgaaagcg atcgcttttg
    20701 tatttggtag tctgaccaca ttgcctgcat tcatttccgg tacttttttc tggtacttag
    20761 caggactggc tgttagtcta gtcgtagcaa tgatcactac tctcgttagc gggtttgatg
    20821 aagacctaat gtcttatgaa tagttaatga aaaagactga agtatgagat gtttatcaca
    20881 tatttcagtc tttttctttt taggatcatt atttttataa atttttgtga tactttccaa
    20941 actttggtat aatttacata tgcataacca tttttatccc cacttgttta tccccaaaca
    21001 aaggtggtct atgcaataaa atgaacgtca aagaaaaacg aatgaatgct cgttgcttat
    21061 ttacaaattg ttttatttag aatacacaga cagatttatg aaaagaaaaa gcttttcgac
    21121 acgattatct gttccaaaaa atgtctaatg aatgctgaat aagacagaaa atcaatgaaa
    21181 ggaaaaggac tagtttatta tgaaaaataa aaaaacgata cgtttgctag ctgctacttg
    21241 gctgattgct ccgtctgttt tgtcgaccaa taccgtattt gccgtagata atcaaatagc
    21301 tgatcaaaca gtcacaacag atacgactac atccagctca gctactagtg aaacaacgga
    21361 ttcttttaca gaagacccaa ctggaacaac taactcaaca acaggatcga ccgagccaac
    21421 tgaaattgat ccggctgatc catcagaaaa tgaaaagcca gccgagacat attcccttac
    21481 aattgctcct tctttgattg gtaagatcca actgactgct tcgacagcgg attcccctga
    21541 acagcaacct ttggatatta cggaaagtgg aacggtagaa ggtttattag cggaaacgaa
    21601 ggtaacttac actattacgc cagctgaagg tgaacaaatt gcttcagtcg ctgttaatgg
    21661 tgccaaagaa gtaaattatg atgcaaagac ctttacagta ggtacgaaca atttatctat
    21721 tactgcagaa tttaaagctg gaccggatac ttcaacagag aaaccaactg atcctagtga
    21781 agaagtccct agtacaccag atccggaaac gccaaacaaa ccagatgtgg gtaataacaa
    21841 caataataat ggttcaaata attcaaacaa taataacaat aattcgaaca gcgggaattc
    21901 ttcgaatcaa acaaacccat ctggatcgaa taacaatgga aatactcaga gagaaccaaa
    21961 ccatccaaac aatggttcat cgaatggcgg aacgaacaat gcttcatctc gtccaaatac
    22021 agcaaatcaa agcggtatag agaatccatc ttctgcttct tctgattttg ttgtaaaatc
    22081 accaattgat gctgtattgc ctacaaatac aacgaatgtc caacaagcaa tcgttcgaga
    22141 agcattcaag catcttggaa aaccatatgt atggggagca aaaggtccaa atacttttga
    22201 ctgttctggt ttgacttatt acgtatatat gaaagcaacc ggtcattata tcggtggctg
    22261 gacaggcgaa cagcaatatg caggtacgca gattccagta agtcaggccc aacctggcga
    22321 cctcgtcttc tggggacctt cttccggtgt tactcaccat gtaggtatct atattgggaa
    22381 cggacaattt atccatgcgc ctcaaccagg agataaagtt cgagtaactt ctatttcaga
    22441 tttcacgcct gattttgctg tccgagtaaa cttagcaggt ttacctgctg cttcaggttc
    22501 gctgacaggc ggctcatcta ttttagatgg attagatggc tcgttccatt tcagtcaaaa
    22561 ccaaacaact gatcagttct tgaaaaagat tgcagatgat gcacaggaaa tcgggcaaaa
    22621 agaaggaatc tacgcatcag tcatgatggc tcaagctatc cttgaaagtg gatctggaaa
    22681 cagcttatta tcaagcgaac caaatcataa cctttttggt atcaaaggat catataaagg
    22741 aagcagcgtg accttcaata cacttgaaca agatagttcc ggacaaagct accaaatccg
    22801 cgcacaattc cgtaaatatc cttcttacaa ggaaagtttg gaagactatg ccgacttgat
    22861 caaaaatgga ctgactggca atcctgattt ctacaaacca acttggaagt cggaaacaaa
    22921 agactacaaa gaagcaacca aatatttgga aggacgttat gctacagatc gccaatattc
    22981 acaaaaattg aacgcgatca tcgaagcgta cgacctgaca aaatatgatg aaccgaaaaa
    23041 agaagcagca acaactgaag aagcaaacgc tgaaatcctc aatatgtcta agcgattcga
    23101 tgtgccaatc aaatggacaa cagatggcgt gccaatcaca ggattagaat tttcaacgat
    23161 aaaacgcgtc aacaacgctt cttccatctt ccgtgtcgct tcaatctggg atttgtggaa
    23221 caattttacg gcaagacaga taccagaaac aactactcgt acagttaaag ctgcaactgt
    23281 tcctgtacta accttgttaa gtttagacaa atcattctgg cttgatctgc ctttaaagaa
    23341 atagaataaa ttaaaaaggg gatgtgacac tgtcacactc cctttttaat ttattctatt
    23401 tctttatatt tattttgtat tccgttacaa actcgcgtac ttggttaggt tccagagtga
    23461 catctccaaa actttcaagt cgctccgctc ctggcagttc ttgagtctca aatgtgatac
    23521 cgccatgatt tactaattta ttcccgcgca tttccggtcc atcttctcca aaatttgctg
    23581 taaaaatcac gattcctgaa gcatctgttt ttacttggat cgatactttt tcatctgggc
    23641 ttgtcaattc cgcagcattt agtaaaccag attgtttttt caacaaaaat ggatgatcga
    23701 tgccgcccac tatcattgat tggcgaaaat cactggcaaa gacctcacct aattttttag
    23761 gacgttggaa gtcaaatgct gtgccactta ctgaaactac ttctcctgta ggcgttgtat
    23821 ctttattgag cgtagcaaat aatcggctat tcaaccaaag tatatgttcg tcgattgact
    23881 gtgctggatc accggttaaa ttgaaataga catgatttgt aggattgaat actgttttgc
    23941 ggtcactgat cccttggacc gtcactcgcc atgtatcatc gttcgtcagc gtataccgta
    24001 cttctacatc taaagttcca ggaaatccgt tttccatatc tgggctagtc tgatagaaaa
    24061 tgaccgatgc ttcgttttca ccattgatga ctgtataatg ccactgcttg gcttcaaagc
    24121 taggtgcacc accatgtaaa caatgaccag tgtccgggtc tgtttttact tcgaacgttt
    24181 catcatctaa tttgaatgtg ccattcgtaa tccgtccagc catacgtccg attgaagcac
    24241 cgatataagg gtctttttct agatattctt ttgcttcatc aaaacctaag atcaactctc
    24301 gtttttcatt atcgattatt gtttgaaaat gaacaacccg tgccccaaga tcactgacag
    24361 acaaagatgt cccattttga ttcgtgatcg taatcaagtg atagttttcg ccaaaatttt
    24421 gttcattgat ttccatgttc cttgcaccct cttttctttc atttgcactg attccagtat
    24481 acatggaaac cgttttctta acagcttatt ccgtgagtaa cgagaaaaaa tttactatat
    24541 tctaataaaa gaccgtgaaa aaaaggttgt gacaaaaata ttgtcacaac ctctttttcc
    24601 gaacaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
    24661 gaggagctat ttttgcttgg ctgttcttat tacttgatgc aggacagctt tttcacaatc
    24721 tctttttcaa tacgattcca gtatataacc atgtcgtcag tacatagtag atgaaataca
    24781 atacgaagaa tatcgtaaac ggaacccaaa tatctttgta tggattttgc atgaatagac
    24841 caaacatttg taatccgaat aagacatgga ctacacctaa gattcctggt gctaagaata
    24901 acacgcctac ttctctacgg atcgattgtt taagcaaccc gcgtcgtgtt cctatctttt
    24961 caagcatttc ataacgagca atgtcactct tgcttcctga caagatcttg aacatcaaac
    25021 agctagcaag catggtcaag aaagctaaac ctaggaagaa tcccataaat tcaaaaccag
    25081 agaatagacc attatacact tcatacattt catatttctg gttgctgtac tctacttttt
    25141 gcaaagatgg attgttcatt ttattttgag caacaagtgt ttcgattggt ttcaggtttg
    25201 cttggaagtc ttttacctga actagctgta aagtggattg tggcaagttc agttgatcaa
    25261 attctgattg actgactacc ttgatttctt tttcttgctg ttccggcaat tcatagcttc
    25321 gaagttcctc ttgtacagtc agttcattca tttttttagc aggaactttt tctttcgtga
    25381 tcttcggcgg tgttccatct tcatgtttta tcatgatcaa tggttgagcc gcaaattcac
    25441 ttgcattata gtaaatcgtt cccgcatctt ctttttgagt ataagaagca gaaagtgttg
    25501 gtgaaagttc tttgattttt tgctgatctt ctgcccgagg attattcatc actagatcat
    25561 atccagaatt gttttctgtc aagtctgcaa tttcattttt aaaacctaat cctaccgtca
    25621 aagcgcccag tgccaaggca aacagcatag cgaccattga gagcatttgt gtatattcac
    25681 gaatacgaaa gtttaactgt gacaatgtga aattgttcaa ttttttcaat gaaatctgtt
    25741 ctgatttctt tagtaatgat aaaaagaaaa tgatcacaga atggaaaatg aaataagttc
    25801 ctaatacgat cgtaactaag gctaccccta taccaaagac ttgataggtc cccacgtgtt
    25861 tcatcatata gtaaccgcag cctagaaaag caattcccag tactacttca aggaaaaaca
    25921 agaatttatg ctgcttgatt cttgttggtg tctgatccgc acgaatcaat gctaaaatcg
    25981 gttttttgac aatcgaaaaa gcattgacaa cagcagctaa taaaaacaaa acagcaaaaa
    26041 acaaaacagt gatcaacaac ccgtgaatac taaaaggtgt aaaatgcgaa atattgagat
    26101 ctaactgatc cactaataat cggtggacga tacttgtcaa tcctacaccg acagctgaac
    26161 ccactaaagt agctgcgatc cctactaaga atgtttcaga aaaaatcatc tgtgcgattt
    26221 ttctgccttt tgctcccagc atcataaaca ttgcatagtc tttttgccgc atcgtcatca
    26281 aaaaggaatt tgcatataaa atataaacga atgtgatgat ccctagtaaa acagaaccga
    26341 atcggaaaat gataactgta gccgcaacga tcgtattact tttcaaaaaa gcttcgttgc
    26401 ttgccattga ttcaaacatg taaaaaatag cagatgccat aactaatcct gagaataaca
    26461 ccaagtagtc tcttaaacgg ctcttgatcc cagtaagtga taatttccaa agcatagtca
    26521 atctccccct actgttccaa gtttccaaga atagatagta tttctcgata gaaggaatca
    26581 cggctttgat ctccacgttt cacttcttga tggataccgc catccttgat gaataaaata
    26641 cgttggcaat aacttgcaga aaacggatca tgcgtcacaa gaagaatcga tacatgatct
    26701 tttgtattca attcgtccat cgtatctaaa agatcccggg cacttttgga atccagcgca
    26761 cctgtaggct catcccctaa tagaatcgtt ggttgtgtga tcaatgcacg tgcggcggcg
    26821 acccgttgtt tttgtccacc ggaaatctca gaaggatagc tttccaaaat atgagagatg
    26881 gataaacgtt ctgcaatttt ttggacacgt tgcttgattt cttttggtct gacgccttgt
    26941 aaagaaagag gaacagcgat attttcttgt gctgtcaagc tttctaataa attgaaatct
    27001 tggaaaataa agccaatctc attcgcacga aaatctgcta gttgatttcc ttttaatgtg
    27061 gtcacatctt tttgattgat tcggatatgc ccatctgttg gtttatctaa tgtggataaa
    27121 atattcaaaa gtgtcgactt accagaacca gaagcgccca tgatcccaat aaattcgcct
    27181 ttttctacat cgaagctgat gccgcttaat gcttgcgtct ttttctcatt gttttttccg
    27241 tatacttttg ttacattctg tacttcgact attttgttca tttctttccc taccctttaa
    27301 ttagtttttc cagtgctgtt ttcggcacgt cttttttttc gacttcttca tacgaagtga
    27361 cacctttatt tttgttccag gtgattttta aatatgctcc tttacgaagc ggtcgagtca
    27421 attgtccctt gaagtccagt tctttttcct taccctcttt cgtatagcca ggcaggatat
    27481 atctatagtt gactgtttct tgtccagagt ctgattttga aactgttttt gtaccatctg
    27541 tcgtaatttg tacatagtat tcttctccac ccataactac gtaatctgcg attttcaccc
    27601 ctgcaagtac cacaacgatg aaaacaagta gaccgatcag aagttttttc actttttccc
    27661 cttctctctt actttatata ctgattatag aaaaaagcga acaattctgc cttcgaatca
    27721 actatcattc ttctttcaat tttgtcattt tctttaaaac aactaaaaaa agagttcaag
    27781 acaataatcc gtcctaaact ccttcttcct atcttacaaa aagtatgtca cttacggtct
    27841 ttcatgtcgc ctgtattgat aacaggttgc gttcctttgt ctgtaatgat tgagacaagt
    27901 aaattattga tcaattggac tttccgatca tcggtgaaat tgatttcttg tccatcttcg
    27961 atttgctcta aggccatttg agtgattgtg acagctcctt caacaatggt ttgtcgagca
    28021 gacaaaatgg cttttgcttg ttgacgttgg agcatggcac tagcgatttc tgttgcatag
    28081 gctaaatggt tcaagcgtgt ttcgattact tcgacacctg ctactgctaa acgctcttgc
    28141 agctcttttt gcaactcgtc agacactgcc gtcgtgtttc ctcgaagcgt caaatcattc
    28201 tcattgaatg tatcatacgg gtattggcta gcgatatgcc gaatggctgt ctcactttgg
    28261 atttctacaa aatcttgata atacgctaca tcgaataatg cctttgctgt atcaacgact
    28321 ttaaagacta ctactgctga gatttcgatg gggtttccat ccaaatcatt gacttttaaa
    28381 acagaactat tgaaattccg aactttcaac gaaacagtca ttttttgtgc caaaggtatc
    28441 gtaaggaaaa agccattttc tcgaatcgta cctaaatatt gtccaaaaaa caaaattact
    28501 tttgcttgat taggactgac tacagttgcc gaacttaaca acaataacgt gatcaaccaa
    28561 agaaaaatac ttaaaaaaag tgcccaaact ttcgcttgcc acatacctaa atagaagaaa
    28621 aacaatccaa ctaaagctaa tacgaccaat ccgacaatcc ctgcataacc attgatataa
    28681 aatgctcttt tttctctcat gctacttcct gcgctccttt atccttcaat aaatatctaa
    28741 tgttactttt ttaatttttt tttcgaatac gcgccattac accttctagt tctaactctt
    28801 tgtcttcagt gatgtaccaa ccgcttgcct gtgtccctcc ggctgcctgc ggtaaataat
    28861 ccaattttgt tcgaacccag tgcttttctt ctgtaaaata gtcttctacc aagacctcta
    28921 gatccatccc tgctgaaaga ggcaaatctt cccgaacaga tttaaagata aatcgttcct
    28981 tcttcccatc atacttcaat attcctgtat cgataatcgg taaagaataa taatccagct
    29041 ctttcttcac cgtcaataaa tgtttgaaaa cttcatggta cacttgctct aattggcgac
    29101 cttctgaatt gttcgggtaa ttaaccatca ctttttcttt gtctggatca tagaaggttt
    29161 ggcttagttg agtgatataa gtatgtaatc tttccatttc tgtttttaac ttttctcttt
    29221 gaccttcttc taccttcata tactctttca ccctgttttc tttttagata ttttctttct
    29281 tattttacta tagttatgtg cgttaagcga tgaaaaacag caaagattgg aaggatcttt
    29341 gctgtttttt cttaaaatat ccagttgagt acaggatcaa tctgtttcat tgtcctcttg
    29401 atccgtcgta atattgatcg atgtgtcttt agcagtgatc tttagctttt tctgtccttg
    29461 ttctaacaaa gtatttgtta ttggctggta gtctttttca atataagatt caccagacgt
    29521 ttggatagta tagccactgt tttccatctc atgcaaagat aaattgccac cgtttaggat
    29581 gacttttcct tctttgctga tcaaactgtc ttctagatgg aagttgctgt tttcacctgt
    29641 gacgatcaag ttatccacat tggcctcatc taatcgaagt gagcctaatt catctttcgc
    29701 agttattgtt cctcctgtaa attcattgaa cgatacccag ccatcatgtg tcttagtatt
    29761 cacttttttg attgatactt cgttgatatc aattgcacta ttattccctt cgattgtcaa
    29821 ttcagataat tcatgatctg cagggacaac aatagtgatt tgtgcacttg tgtcttctaa
    29881 actaatgatt gcacctgttg attttttgac tgcattcaca gtcaacacgc catttttctc
    29941 ctttatttct ggcttttctg tcttatcata agtcgttttt atctcaaaat ccaatccgcg
    30001 tatgaccgaa actgtttgat tttctgcatg gatgacgatt ttgtttgcat ccttgatttt
    30061 ttttgtctca gtaaccatat cgatcaaccg aggaccatta tcccaagtca gactcttttg
    30121 agcgcctaat gtataagttg cacctgctag gaggccacca gtaagcatca gaccaatacc
    30181 ggtaatcaaa aaatgtttca atctcaattt atgtgtcctc cttcaaacgc ccgctgaatg
    30241 cagatctctt tttgtaataa aacgtcgtcc catccattta acaaagttca tcatgacata
    30301 aaaaagccct tttgttcctt tcagcaacag tggcgaaata aatactgcgc ttcctgtggc
    30361 gaacagaccg acaccgataa agaataatcc actcagaatc gattgtccaa acacactgat
    30421 tcctgagatg atcatgaata ggccaacacc taagatactg atacataaaa catagatact
    30481 aaaaatgagc gctagtaaag caaccaatag tcccccaata accaatataa accccagtgc
    30541 tagtggaatg aggacaggag aagcaaataa accgataatg attaaccaaa tcaggcgaat
    30601 ttgtcttttt ggatttttca ctaactcgtt ttctgcttgt tcttcttctg aaaagtaact
    30661 gatttttaat tttaaggcaa actgctttgg tgtaccatat tcatcaatga tctgatctaa
    30721 tgtttttcca gaatctaaaa aatattcctc ataatactgg atcaaatcaa aacgatcgtc
    30781 ttcaggaatt tcttgaagat gatccacaac ttcattcaaa tattcattca tttgtgctct
    30841 cctccaataa ccggtctaaa gaatctcgaa aaacaatcca ttcatcaata atttcttcta
    30901 aatgacgact tcccattggt gtcaacgagt aatatcgacg gtttctgcct tcaaaaggtt
    30961 catcataggt ctcaagatat ccttcttttt tcaatcttct caaaaccgga tatagtgtag
    31021 attcactgac agagagactt tcttgtacga tttttgttaa ggcataccca tacatatctt
    31081 ccttgcttaa tgctgctagc acagtaccat caagaagaac agttggaatc tggatgtgca
    31141 ttacgtcacc ttctattctc aagtgtaata ttttatttca atctatatta tacaacatat
    31201 aatatagttt caaaatcagt ttgtcaaata caaaaaaact gactaaaaga tttgatcctt
    31261 tttagtcagc aaaaaatggc ttctctttga tttttattat aacagtggca taatccaatt
    31321 tgtccacatc cagctcagtg gcatgataac gatcattgca ggtatcatgt cagctactgg
    31381 gaacattttc acgttgatca ttctaaaacc ggtagcgacc atcaagaatc cgccgcaagc
    31441 tttaaaatct aagatcatgt ctggtgtagt caatggataa ataaattttg ccaataagaa
    31501 caaaatataa aaaataatga attggggaat tgctatcaca gaaacgacat accctaaatt
    31561 acacgcaaaa atagctgctg tgaaaaaatc aaggacagat ttagaaatca aaatcgtact
    31621 gtctcctgtc atcccagaat ctaaacttcc atagatcccc gtaccacttg cacaaaatag
    31681 aacaatgacc gtcactaatg tgctgataaa ttcatcgtgg gtcataccaa gcttttcact
    31741 agggaagatt ttagaaatag gtacttgcat cagcgcagcc cccttattta tcccattacc
    31801 taaatgaaca atcaaaccaa tcgctgttcc tatcactaaa gcaaaaataa cagccggcat
    31861 atttttcatc ggcgcaatgg aatagattcc catccccatc gaacacacac caaaaaccat
    31921 cgtcagctgt gctttaaaat gttcacttaa tttatttcct actaatccgc caattagtcc
    31981 gccaaacaaa acggaaccta cattgatcaa aactccagtt ggcatcaaaa aactcctctc
    32041 tttttctacg aagcgaaaaa aatcagaaaa atttctgtaa ttattgcttt cgctgcaaga
    32101 aagattgtaa cagacagaga agattacacc aattcatttt ttgtgttaaa gtattcttta
    32161 ttggaataag taggaggaat atgatggata tccataaatt cgaagttttt cttgatttag
    32221 ccgaaacaat gaatttcacc aaaactgctg atcgacaatt caccacccaa ggaaatattt
    32281 ctaaacagat tctttcgtta gaaacagaat tagacgtcaa attattcgaa cgcgcccata
    32341 gaaaaatcga attgaccgaa gctggaagct tattgctccc atatgtaaaa aatgtcgtag
    32401 aacaatacca tgctatgcaa gaagtgcttg aaacctatac gaaagacaac aatctttcat
    32461 taaatatctt aacgataccg acaatgatca attataaagg tttttccaaa atcaccgagt
    32521 ttttgaaacg ccatcctgaa tttacagtcc agttgaaaga agtggaaagt gtcgaattaa
    32581 cttacaatga cgccaatgaa catagtgaca cgatccactt tgctagaagt ttccaggccc
    32641 caccaagttc gattgagtgg ttgcctactg aaacagacga ttttgtagca gttctaccta
    32701 aaaatcatcc tttagctagt aaaaagaaat tggatctaag cgaactgaaa aaggagaact
    32761 ttttgctcct tggccccact acgaacttgt atcaacctgt actagatctg tgtcgcgaag
    32821 ctggatttga accaaaaatc tcttataaag gagcaagaat cgacttgatc atcaatatga
    32881 ttgcaaatga catggggatt gctattgtta tggaaaaaac agtcaagaat ctattaaaag
    32941 aaaatactgt gattctccct atttctccaa caacagagag ttatcttgca tttactcgta
    33001 aagtcggtga gcattctttg gcttcagata cattttggga gtatttaaat caagaagaaa
    33061 actaagccct acattttctg tccatctatc cacccaaaaa aagagagcga cgcatgactt
    33121 tcgctctctt tcttgcatca tgtccttgtt tcaatgacac ctctaacatt tctcgtagtt
    33181 caataagaaa gcagcgattt ttatgccaaa agaaccgtaa gaacatttcc ctaatcacaa
    33241 aaaaacgact aaaaacccct aaaaaccttc aatagaattt ttaggggttt tattcttttt
    33301 cctattgttt acttaaccgt atcattgctg tcgttcctac tccttcgata ctgctataat
    33361 caatacgtcc atcatgcagt tctagaattt ttttaaccag tgaaagtcct agcccattcc
    33421 cagaaatttg acgacttgta tctccttgat aaaatttttc aaacattcga tctctggtct
    33481 cttcattcat tcctacacct gaatcggtca cttccagtac aatctctgtg gctgtctcaa
    33541 atagtttgat atgaatctgt ccattgattt gattgtactt tatggcgtta tccatgatat
    33601 tcagccacac ttggtacaaa aactcttcat tgcctgtgta atttactgca gcaagttcga
    33661 tatccaattc tagctgttct ttctcccatt taggctgaaa aaaaacgatt acttctctga
    33721 tttgttcatc taatcgatac tctttctttt ccagttggat acgctggttt tccagtttgt
    33781 tcaattttaa gatgttctct gttaattgtg aaagctgcgt gatactttcg ataatccgat
    33841 ataaaaatat ttggcgttct tcgtcagaga gatttggggc ttgtaacaat tgaacatatc
    33901 cttggattgt tgctagtggt gttttgaatt catgggatac actggacaca aagtcattcc
    33961 tcagtgtctc gatactattg agttcttgga ccataacgtt gaaatcttta tacagttgct
    34021 gtacttctgc tactttctgc tgttcatcca ttcgaatcga aaaatctccc gttgctacca
    34081 gactcatatt tgtccctagc gcactgattg gatgcaggat acgccgccca acaaaaatcg
    34141 atattcctgt acccacgacc atactgaacc ccgcaaagac gaataacggg tataacgttg
    34201 gttttccagc cgattcagca ggcgagatag aatgtcgaga caataagaaa ataaaaccga
    34261 gaaaacacag cagtgtcact aagatcgaaa tgaagacaat actagcaaaa tagatccata
    34321 attgtgaaaa tagctttttt ttcatcttta aataaccgcc ttatagccta gtccccgtac
    34381 tgtgatgatt tgaaaatctt ccgtcttttc aaaacgggtc cttaaacgtt tgatatgtac
    34441 atcgatcgtt cgttcttctg tattcgtatc taatccccaa atatcatcca tcaactgttg
    34501 gcgagtgaag atcttgttgg gataagacag taatttatac agaagcagaa attctttttg
    34561 aggcagaatc aacgaatgct gatcttgtaa cactgtataa gcatctagct ctaattttgt
    34621 ttgtccgatt atcaagctac tgctgtaatt gatctttgaa cgtcgaagca atgcttccac
    34681 acgtaggatc atctcattga catcgatggg ttttgtcata taatcatctg ctcctaattt
    34741 gaacccgcgt ttcttatcct caaaatcggc ctttgccgta atcattagaa caggtgtgtc
    34801 aaatctggtg ctacgtaatg agtctagcaa gtcatagcca tccattttcg gcatcatgat
    34861 atctgtaatg attaattcta tacgtgtcgc ttctagtttt ttcagcgcat cctctccatt
    34921 caatgcggtg atgactgaaa agcccgctcg ttccaacaca gaacggtaaa gcaactgtaa
    34981 ttgcggatca tcttctacta ctaaaatacg attcattgat ctccctctgt tttttgaaaa
    35041 atttctagat tttttcttgt agttcatctt cagttaacat ttatttcgta tactacgatc
    35101 attgttctga tatacttatt agtataccga atatttcaat acgggagaag ggtgattatg
    35161 cttcaattaa aagatataaa aaaatattat aaagtcggcg aaaccaccac caaagcattg
    35221 gatggtgtgt ctgtcgcttt cagacaaaaa gaattcgtag cgattttagg tcctagtggt
    35281 tctggtaaaa cgactatgct gaatgtgatt ggtggactag ataattatga ttccggtgat
    35341 atggtaatca acggaaagtc tactaaagac tttaaggaca gtgactggga tgcctaccga
    35401 aacaactcta tcggtttcat ttttcaaagt tacaacttga ttggtcatct aggcattatt
    35461 gaaaacgttg aacttgggat gacattaagt ggtgtttcta aagacgaaaa acgaaaacgg
    35521 gcagaagatg cattgcaccg agtcggcttg acaaatcata tgcataaaaa gccaaatcag
    35581 ttatctggtg gacaaatgca gcgagtggcc atcgctcgag cactagccaa tgacccagat
    35641 atccttttgt gcgatgaacc aaccggagcg ttagatacag aaacaagtat ccagatcatg
    35701 gaattgatcc aagagttatc gaaagaaaaa ttagtgatca tggtcacaca taatcctgaa
    35761 ctggcccatc aatacgcaga tcgaatcatt gaattttctg atggtaagat cttaacagat
    35821 tcccatcctc acatcgaacg accaaaagat gatcaattta acttacgcag aacaaagatg
    35881 agtttttgga cagcgttaaa gctttccttc aacaatatcc gaacaaaaaa aggacgtact
    35941 ttcttgacct cctttgcttc cagtattggg atcatcggaa tcgcgatcgt tctgtccctt
    36001 tcttctggat tccaaaaaca aatcgataac acgcaagctg aaacgatggc gaaatttccg
    36061 attactattt ctaaggtaac gactaaccag actcgtgacg atgccggatt gggtgcaagc
    36121 aaagcggatt atccagacag caaaacgatt actgcaaaag tcagcgaaga agataaagca
    36181 cagcacacga ataagattga tcaaacatac gtcgattacg taacagacat cgatcctaac
    36241 ttaagtaata atatcggatt tacccgtact acaggaatca atcttttacg tgatgtaaat
    36301 ggcaaagtac agcctgtcag cttctcgaat caaaatcctg atacggaatc tctttcttta
    36361 tctagtacga tgtcagcaat gacaggtgtg ggagtgtcaa gtttccctac acagcttgac
    36421 actagcaaag aaaacttttt aaaagacaat tattctttac ttgccggaag ttatccagcc
    36481 tcagcaacag atgtcgtgct gatcgtagac ggcaataata atactaatat caacgcattg
    36541 aagaatcttg gatttgatgt gaaagaagac gaaaaacttg attttgatga tatcgttggt
    36601 acaacattca aattagtaaa caacaatact tactatacaa aacttccaac tgggaatttt
    36661 atccctaata cagattatga tgcgatgtat cagaatgctt ctgatgaatt gaagatctcg
    36721 gggatcctac gcgtgaaatc ttcttctaca atgaatctct tatctccagg aatcgcttat
    36781 agcgatcaat taacgactca aatcgtcaac gaaaacaaag aatcagaaat cgtcaaagct
    36841 caaagagaca gtggtgtaaa tgtcttaact actgaaaaag ttgacgaaaa tgccaaacaa
    36901 acattgctta gttatttagg tggagacagc ttgccttcaa gtatcatgat ttatccgaac
    36961 aattttgaag acaaagaaaa gatcttggat tatctagatg attacaataa aggcaaatca
    37021 gatgaagata aaatcattta tactgattta gctggaacga tgactgaact gactggcgga
    37081 ttgatggatg ctatcactta tgtccttatt gcttttgcag ggatctcttt agtaacaagt
    37141 atgatcatga tcagtatcat tacttataca tctgtgattg aacggacaaa agaaatcggt
    37201 gtactaaaag ctttaggtgc acgtaaaaaa gacatcactc gcgtatttga cgcagaaacg
    37261 tgtattttag gtatttcttc tgggatatta ggtgtgttta ttgcatggct agctacgttc
    37321 cctatcaata gcatcttata caacatgacg gatctaaaaa atgttgctca actaaatcct
    37381 gtccatgcga ttattctagt gatcgtctct actatattga cgatgcttgg cggtcatctt
    37441 cctgcacgaa tggcagcgaa aaaagacgcc gctatcgctt tgcgtgcaga ataaggttgt
    37501 gacaaccaca tttagatttg agtcataaga gcgctgaaat agtagaaaac agcaagaaac
    37561 gaggaagtaa tatgttcctt gtttcgcgct gtttttttta attatttttt ataattctgg
    37621 tatacttttc tttcctttgt accacaactc ttctggcgta tacctgtctt ttccatttat
    37681 gaatactgtt ccatctgctt caatcgttac ttcagtccca atccacatat tacgagaaag
    37741 tgcgagaaga ttttttcttt ttcctcttcc tttttttcca aattgcaagg catcggttat
    37801 ctcttctgtc caatgagatt tcacgtattt tacttctccg ttttcaaatg taactttcaa
    37861 aatattcgct atattttcca cttttactgc tttaggaaat gactcctcca tttttttcac
    37921 tttccttcct ccaacactaa gtatgcgctt tcttttagtg ataagtatat catagtttat
    37981 tctattatca aaaaaacgca tccattccat tgtagaatac tttaccgaac aagttgaatt
    38041 gttttcctct ttttcacctt ttactataga taggtaaaga caactaagga ggaatctctc
    38101 atgacagatc aaccgtatga tttaagaaaa gtcatcgaag aactaaaaac acttccaggt
    38161 caatatcacg aaacagatat tgaaatcgat cctgaagcag aattatctgg tgtttaccgt
    38221 tatatcggtg ctggcggtac agtaaaacgt ccgactcaag aaggaccagc tatgactttc
    38281 aacaatatca aaggtttccc cggcacacgt gtgaacatcg gaacaatggc tagtcgtaaa
    38341 cgtgtaggtg aaattttgca ccatgattat cgaacacttg gtcgtttctt gaaagacgct
    38401 gtagaaaatc caatcaaacc tgtgaaagta gacaaagcag acgctccagc acaagaagtc
    38461 gttcatctag ctaccgatgc agacttcgat attcgcaaca ttttgcctgc acctacaaat
    38521 actcctgaag atgctggtcc atacatcaca atgggtgtag tcttaggatc agatccagaa
    38581 aaaacaatga gcgatgtaac gatccatcgt atggttctag aagataaaga tacgatcggt
    38641 atgtacatca tgcctggcgg acgccacatt ggtcacttcc aaaaacaatt cgaagccttg
    38701 aataaaccaa tgcctatcac tatcaatatt ggtttagatc cagcaattgc cattggcacg
    38761 actttcgaac cacctaccac tccacttggc tacaatgaac tatggtgtgc aggagctttg
    38821 cgtaacgaac ctattcaaat cgttgatggt atagcagtcg atgaagtggg tattgctcgt
    38881 gctgaattca tcattgaagc agaaatcatg ccaaatgaaa caatgcaaga agatatcaac
    38941 acgaactcag gaaaagcaat gccagaattt cctggctata ctggggatgc taatccagct
    39001 gtacaagtag tgaaagtcaa agcagttact caccgaaaag acaatccaat catgcaaaca
    39061 acgattggtc ccagtgaaga acacgtatct atggccggta ttcctacaga agcaagtatt
    39121 cttgccttgg tcgataaagc tattccagga aaagtagtaa atgtctacaa tcctcctgca
    39181 ggtggtggta aattgatgac catcatgcaa atccgtaaag aaaacgaagc ggatgaaggt
    39241 attcagcgtc aagcagctat tcttgcttta tcagccttca aagaattgaa gacagtcatt
    39301 ttagtcgatg aagatgttga tattttcgac atgaacgatg tagtttggac tttcaacact
    39361 cgtttccaag gagataaaga tatcatcgtg atcccaggaa tgaggaacca tccattggac
    39421 ccatcagaat taccagaata taatcctggc tctattcgtt tccgtgggat aagtgcaaaa
    39481 actatctttg acggtacagt tcctttcgat atgaaagaac attttgtacg tgcaaaattc
    39541 caagaagtag aagattggga aaaatatttg aaataatgaa tgatctgcaa ctgttttgac
    39601 atcataaaaa ggagtgcgga aaatggaaca attggcacaa cggaagaaaa aaatcgtgat
    39661 tggtgtaagt ggcgcttcag gcaccattta tgccatcaac ctagtgaaga aactaaaaga
    39721 atatccgatg atagaaacac acggaatcat cagtccatgg gcaaaacaga acttaaagct
    39781 ggaatccgac ttatcactaa ctgaattcga aagcctgttt gattaccatt attcaaacaa
    39841 agatttaggt gctgctgtcg ccagcggatc atttttgacc gatggaatga tcatcgtacc
    39901 ggcaagtatg aaaacagtcg caggtatctc tgttggttta ggcgacaacc tgattgcacg
    39961 tgctgctgat gtaacaatga aggagcaacg aaagttagtc attgttcctc gtgaaacgcc
    40021 attaaatgcc attcacttgg aaaacttgac taaattagca cgactaggtg tgcaaatcat
    40081 accaccaatt cctgcttttt ataatcagcc tcaaacgatc caagatcttg ttaatcacca
    40141 tacaatgaaa cttttggatc aatttgaaat tgtaaatgag cattcaaaac gttggcaggg
    40201 tgattaatat ggaaaaagaa acgatagttg aagtaaccaa actaggtttc acgatccaag
    40261 ctgctgttaa agtaatcggt aaagacttat tgatcacttt aacaggcgga gatacaccac
    40321 atatcggaac agtcactaca ttttctcgtg atacagagat gcaaactgta cgtttcccta
    40381 gccacgatgg tcggttccat aaagacgatg tcttagccga aaaaatagct gtgatcatca
    40441 aaactgccgt tccgggaagt tgtacgatca cttccggtat ccatgttgac catatctcca
    40501 atgcacaaat caacgcctct tttccgatgt cagaagagtt aggttacaag attttagatt
    40561 ggttggaaaa aatagatttc catatcaaag aaccagttta ttataaagat ggtgaaaaac
    40621 cattatgaaa gaagaggttg tgaaatcagc gttatgacct gagtatgaga gaaaaaaatg
    40681 gaaaaatagc ttctcatatt ttgccgtttt tgagctaata ccgagggaca gctgattgaa
    40741 cgccgtttat caagaggttg tgataaagcc gcttcgtttc gagtagtaag aaaaattttg
    40801 taaacatggg tttccacatt tttgcataaa tttgacttac taccgagaaa ctggcttttg
    40861 aacaccgttt atcaagaggt tgtgacacgt cttttgtcac aacctctttt atttttgctt
    40921 cactgtaact gctacaatat caccgatttc ttttccgatg tttttacgaa tatcttttcg
    40981 tagtccaagg atgtagcaga ctgaaccatc tggatttttc acgcccatat tcacgatact
    41041 gccttcatac ggcacattgt caaaagttgc ttggacttta actcttccag caccgaattc
    41101 ttttcttata tcatacggaa aaataatata tgccccgccc ttgccgactt cggaagcata
    41161 gatttttgat tcaaaagaat aaatttccct cattttattt ctcctatttt ttacatctag
    41221 tatagcatag caaaaaaaag aaggaactgt gacaaaagtc ttgtcgcagt tccttttttc
    41281 gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatagtc aacaaacatg
    41341 agaagctgtt ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct
    41401 cttccttaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc agatgaaaaa
    41461 tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag ctttttcaca
    41521 acctcctaca tggtgttcaa aatattattc gccttttgct tctttcacta attttgcgaa
    41581 tgcttgttgt tggaattcag cagctaaggc tttcgtacgt tgttcttgct ctgctttgct
    41641 catttttgga ttttcagtca ttgcttttac cataccgtct tgaacataag cttttgcttc
    41701 actttccatc gtactttgct gtttcttcac taattcagct aacttggctg cttggtccgt
    41761 acttaattct acccatgtct ctgggatttt gaatgtattc actcggctgt catattcgtg
    41821 attgttatca gcaatattga accagaattt atacatgtcg tttttgtatt cccaataagt
    41881 agttttcgtc actttttcag catcgccgtc tgtcttttct actttatttg tttcgttatc
    41941 cgtaccagtt gcttttggtt tttcttgttt ttcatttgtt cggtaaagat acactttttc
    42001 ttttccattc cctaaagctt tatacaaaag catatctgct ccttgggaat cagcactgga
    42061 aacaattttt tctgtttttg tttccgttac tttttccatt ccaaagtgat tggacaaatt
    42121 agcgacgatc aaacttagtg aagcgacgaa gattagtccg aaaatcaaag ataaaaaggt
    42181 ttgccatgtt tttttagcga acacgttcgt taaagcaaaa gcaagcaagc tgacaatcaa
    42241 aactccaata atcatgcatt atcccccgct ttcattagaa caggttcttt tttcttcaat
    42301 aaaaaggcaa taataaagcc aagaatccca aagatgatcg ctacgataaa cgcggcatgg
    42361 taacctgaca gtgtggcatt gattgcctga tctttataag ctaatggcgt attatccaat
    42421 acagatttgc ttgggatatt tgcttttgtc acattcgtca acacactgat caaaatagct
    42481 gtaccaatcg agcttgcgat ttgacggaat gtattattta ccgctgtccc atgactgatc
    42541 aagttatttg gcaatgcatt cataccagaa gtcgttaccg gcatcatgac catggagatc
    42601 ccaaacatac ggatcgcata aaggatcacg atatataaaa ctggagtcgt ttttgtcaag
    42661 aaagcaaatg gtgcagttgc cgctgtcaaa atgaacatcc ctgtgatggc taaacgtttc
    42721 gcgccgtatt tatcgaaaat cgcacccgtc acaggcatca tagcccccat gatcaatgca
    42781 cccggcaaca acatcaagcc agaatggaat gctgattctc cacgaatatt ttgaatatac
    42841 aatggtacga ccatttctgc ccctaccatc gccatatttg ttacaccagc taaaacagct
    42901 gcaatcgtga atgtagatga tttgaataca cgaagttcca agaatggatg ttccaaatgt
    42961 aattgacgcc atgagaacaa ggcgatgaaa atcacaccaa caactaagaa accaattact
    43021 ttcaagcttc cccagccgtc atctcctaca ctagaaaatc cgtaaagtaa actaccgaat
    43081 ccaatcgttg ataaaatagc ggataaaata tcaatgctag gatttgacaa tggaataaca
    43141 cttttcatta ggaagaatgc taaaaccaag acaagaacaa cgattggcag taccatacca
    43201 aacaagtcac gccaatggta gttatcaatg atataaccag ataatgtagg gcctaaagca
    43261 ggagcaagtc caataacgat ccctgacatc cccattgctg atcctctttt ttctggcggg
    43321 aaaatagata gcatgatatt ttgcaataat ggcatcgata ctcccacacc gcaagcttgg
    43381 atcaaacgac cgattagtaa tgtactaaaa ttaggtgctg taaagcaagt aatcgttccg
    43441 atcaaaaaga tcgtcatggc agaaagatat aaatttcttg aactgaattt attgatcagc
    43501 catgctgtta ttgggatcat gatcccgttg actagcaaga aaccagtcgt taaccattgc
    43561 acatcagacg ctgaaatatc gaaagctttc atcaatgtag gaaaagctgt cgtcaataag
    43621 gtctgattca aaacggtaca aaatgtacca attaataaga caacaactaa aagtgtccga
    43681 ttatatggtt ttccgtaaat atctacgggt tggttgttac tcatatttct ccttctttct
    43741 gaattttttc tgaaactact agaatacttt agtcctaaat tcgctatttg tcaacctact
    43801 tgacacataa gttaatacgg atataattaa gggaagcaag aggacaaaag gagtgacaaa
    43861 aatggttggg gcttccctta aacgtttgtt ggaaaatgat caattggttg ttggcatcag
    43921 tgaattaagt gatatcgtag gtgtgtctcc acgtcaatta aggtattggg aacaaaaggg
    43981 attcattcgt tcgatcgaag ctgatgcgaa ttgtcctaga aaatatcgtt tacctacagt
    44041 cataaaagta gaactgatca aaaaatattt agatgacgga tttactttgt caaaagctgc
    44101 agaaaaggca gaaatccgcc aaaagaaaat gcaccatgtc cgtaaagttt tttcaaaagc
    44161 aatcaaagat atcgaattga tcgacgaacg ctatacgatt ttacaaatcg gtaacttcaa
    44221 agaagagaat gctaagatgt acatcattca tgacaacgag acagacgaat taacttacaa
    44281 gctccttcct gtcgatgaaa aagctgacta cgaacagttg ttaaaagaac tctaaacgcc
    44341 actaatctcc taagtaccaa gctttttaat aagtagatgc tacataaata cttgaaaaaa
    44401 ggcctgtttg aacataagaa tatccgaacg gtgatctgcg tttctttcat atagaaagga
    44461 attctccatc actgttcgga tattttttta tttttttcgg ttactttctt ctaatacatc
    44521 tgttagccaa tttgcatatt ctgtttcccg attgatcgca tgcgctaaaa tcaggtagtg
    44581 tccgtaattt tcatgttttt ctttttcagt aggaaagatc gttttttttc gctctaatag
    44641 atgaagtcgt ttttcttcat gaaggtcacg ctgctgctga ataatttcta ctaatgccgg
    44701 atcattcacg tctttgacga aatacaattt cagaacaaat tcgtctcgat taaccggtag
    44761 ttcattggtg ggtgtatgaa tccactctgc caattgttcc ttcccttctt ctgttaattc
    44821 ataaactttc ttttccaatt tttcacctgt gatctctact tgatgctgaa tgattccttg
    44881 ctcttccatt tttgcaagtt caggataaat ctgactatgt tttgcttgcc aaaattctcc
    44941 tatttcattt ttgaacgatt gattcaattc atatcctgtc atcttcttct tacttaataa
    45001 ccctaacaaa atatatcgaa gtgtattttt tcttgccatc agttttccct ccttgacaaa
    45061 tctttaaatc cattctatca taaatgagta atcgaaaaca tgtagtttat tacatgtttt
    45121 aatttataag gagatgctta aacatgaaag aatttaaaga attggaagat tttattggaa
    45181 ctcactttat ctatacgtat gacaatggtt gggaatatga atggtatgcc aaaaatgacc
    45241 atactgttga ttaccgtatc catggcggaa tggtcaaagg ccgttgggta aaagaccaag
    45301 aagctgatat cgttaaattg acggacggcg tattcaaaat cacttggaca gagccaacag
    45361 ggacagacgt ggcattggac tttatgccaa atgaaaagaa acttcacgga atgatctttt
    45421 tccctaaatg ggttgaagaa catccagaaa tcactgttac tttccaaaac gaacatatcg
    45481 cagaaatgga agcagctcgt gaaaaatatg aaacttatcc aaaattactt gttcctgaat
    45541 ttgctacgat cacttatatg ggtgatgcag gtatagacaa tgaagatgtt attagcgaag
    45601 aaccatacga tggaatgcca gatgatatcc gtagcggaaa atacttcgat gaaaattata
    45661 aacgagtaaa ataaggttgt gaaagagagg ttgtgaaatc agcgttctga cctgagtatt
    45721 agagaaagaa acggaaaaat agttcctcat gttttgccgt tttttgagct aataccgagg
    45781 gtcagctgat tgaacaccgt ttatcaagag gttgtgaaat cagcgttatg ctttgagtaa
    45841 taagaacgct agttagaaaa tagcttttca tattttctag gctagtgggc ttattaccga
    45901 gaagcagccg tttgaacacc gtttatcaag aggttgtgaa atcagcgttt tgacctgagt
    45961 attagagaaa caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg
    46021 agggtcagct gattgaacac cgtttatcaa agattgtaac aactgcatat tcacacagtt
    46081 aaattagtga cttcaaaatg aaataatgct cccagcaaaa aattaggctg ggagcattat
    46141 ttcgttattc tgttcatcaa aaatcagcgt tttgattcca tttcatcaca agttcctcct
    46201 catttactgc ttcatctttt tgctgtgttt gaattgtaaa accttgtgaa agatagaaat
    46261 gaatagctga ttgatttttt tgatagactg tcaaagtcag tgttttattg gtttctttta
    46321 tttttttcat tagttgcgta ccgattcttt gttgccggta ttcccttttc acaaagattc
    46381 ctgcgataaa ctgatcaatc accccagcaa aaccaataat ttcttgatct atttctgcta
    46441 agtaaatcac tgcatctggt aaagctttcc taacagattc tacttgattc tcccaataag
    46501 ttgatggaat gaattcatga gaatccaagt tagcatgcag ccaaatcgat agaacagatt
    46561 ccagttcttt ggcagacaat tttgttgttg tgcggatcat tttttctctc cttacttatt
    46621 cattgatttt aaaataaaaa ccattttttt gaagctcttt tacaagtttt cgcccctcaa
    46681 aaaagtatat cttctcttgt tttacttttc taataccaaa agaaacagca actttaggat
    46741 catcagctga aacgattcga taagttccac caggcaattg atccagcttg actttcccat
    46801 gatggtcgct ttgtactgaa aacggcacac catttctagt aagtgttttt ttcttatgtt
    46861 tcaaaatgaa tgaactacca tttaccggtt tccctgacga atcactaata ataaatgaaa
    46921 aatcaagcac ttttttactt aatctatact gatagattag tcgtccgata aacactaaca
    46981 gtatgactac agctattatc gttcctgcaa gaataaacca ttgccgcatg gttctttcct
    47041 gatttccttt tccttcttct ttttttaccg attcgttata aggaatccga tgtcctgtca
    47101 ctagcaaacg atgcgtgttg atcatatacg gcgtacatgt aagcaaggta actaagtctt
    47161 ctccttctga aatcgttaga acagacgtgt catctggcag taccacttgt atatttttaa
    47221 cttgataagc caatttctcc cccaaaacag tcaaaataaa cgtgtctccg ttttctaaac
    47281 gatctaattc ggtaaataac cgtcgattag gtaaaccgct gtgagcagag atcactgaat
    47341 gcgtcgaatt tccaccaagc gggaaagagg ttccttgcaa gacgcctgct ccattttcaa
    47401 ggatcctacc attgagccga tcataaagtg aaatatttaa ttggatctta ggtatattga
    47461 tacttccgat cagccattcc tctaattggc tggatgttgt actttcattt ctgttctttt
    47521 cattgaaagg atcagtacca ggattgaatc cttggtcagt taatcgttgg tttttctcct
    47581 ccattttctt tcgctgcact tcaatttcag ctgcggtccg tttttgtact tgttcgatac
    47641 gtttttgatc aataaaacta tttaaagaat ccacataaaa tggatataaa gcgatcagta
    47701 accctacgac gatacatatt aataaaaaca aacgaaacaa aagtcgacga gactgttttt
    47761 gcttactctg cttcacatgt gtcctccctt gcaagttttt ttgttctttc ttttattata
    47821 tgggttcagg aaaaaaatag aaaatgaaag ccgatgaggt tgtgaaaaag ctgtcctgca
    47881 tcaagtaata agtacagccc aaaaaaatag ctactcatat tttttgttgg gttgggctta
    47941 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa actgcgttat
    48001 gctttgagta ataagagcgc tagtcagaaa atagcttctc atattttcta ggctagtgag
    48061 cttattaccg agaagcagct gattgaacac cgtttaccga gaggtcatga aaaagctact
    48121 ttgtttcgag tattaagaaa aattctgttt caaaacaagg agatgcaaca aaacttatgt
    48181 cgcatcctct ctccttagaa ctttcactta tgtttttctt gtaaaattca taaatatttt
    48241 catgaatttt ctaatttctt gcctacctct cgcataggat atatgatgaa tttatctaaa
    48301 gtttgcaaag gagttcgaca attgaaaaaa tatggcccat ttttagcaat ttttttcatt
    48361 gctttggcat ttctcatgcc tcatttattc acacataaac taattttggg tgctgatgcc
    48421 ccattccatt acaatcggtt ctatgatgcc gcgcaacaaa ttaaatatgg ggatttccat
    48481 tattttcctt ccctttatgg attccagcaa agtggcagga tcgtgaatgc cgtttacggc
    48541 ccatttttcg cttatttaca aggattgatc gtgttgcttg caggtagctg gtttcattat
    48601 cagattcttt caaatttcat tctttatctt atttcaggat tttctatgct gattttactg
    48661 agatatgtca atgttcgaaa ttgggtaagt gtaccagtag ccgttttatt catgagtacg
    48721 tattctattc aatactggtc tatcaaccaa ggattcacta gctgggggac ttgtttttat
    48781 cctttatgta tgatccctat tgctgatttc gtccttcata aaaagttccc tgttatcaaa
    48841 gtcgctgtct cagtgggact tatgacacag atccatttac tgtcttcgat catgttagct
    48901 ttgatgtata tcccttttta tctttattac ttttttaacc agactgaaaa aggaaaagca
    48961 ctgcttcaac tgattaagtc ggtcttgctt tattttgttc tgacagcaaa tatttgggca
    49021 ggattagcta ttgtttacag tgggaataag attcttcctc cctttgttaa cagagaaatg
    49081 agtcaaaagg cgatagatct ggatggcagt tattggctcc attaccctaa ggcctttcct
    49141 atattgatat tatcaggttt tttgcttttt attttctttc aacaaaagaa caccttattc
    49201 caaaaaatcg tgttgtacac aagtcttgtt ttcttttttc tttctactag cttgattcca
    49261 tggacgacat tgatcgaaaa aaatgtgcct tttattagtt tgatccagtt tccatttcga
    49321 tttttcgtgc catttacgct attgcttcta ctttaccttg ccctttcact gaaccagtgg
    49381 tctgaacaaa agtggttccg tcttttaagt atatttggca ttcttatttt ttcagcacag
    49441 acgatccaga acttatacca gcatctggaa aagtgggaaa atgaaacatt tgtcagtcga
    49501 catacttatc tattcgatac gccggaagaa gcaaggaaaa cattcttcag caaagatatg
    49561 tctaccagct tgttcgtttt ccaaaaaacc acacctgact atctgccaat ctataaggag
    49621 acaaaagaca ataaatacga ccggtataac gaagaaatac tagaaaacga aacaactttt
    49681 ataaaaacac atccaggcgg tcttttgaca atcaagtgga aaaacactgg ggacaaaact
    49741 gttcatatcc ctgttatcgt ctacgatcgg actgtgctac agcaaaatgg aaaaacattg
    49801 actgattatg aagtaacaga tatcgggacc ccaatcgtga agcagcaaaa aggaatcaac
    49861 gagctcacgc ttcattatca aacgccaatc tatttctatt tcattctttc tcttactttg
    49921 attggctggt ttaccttgct atgtttattt atttatcata gatacaagct tttacgtgct
    49981 taggcaaaag ggataaatac atccgatcaa aaaactctcc aaacggtata atcgtttgga
    50041 gagttttatt ttaatcagtt tttttatgtt atatgttata ttttttattc gttcacatct
    50101 aattcgattt ccaccccgta gtgatcactg atgattggac tatcgatccc atcaaaaatc
    50161 cgttgatatt ttttaacctg cattccttca gggacgaaaa taaaatctat acgcaatttt
    50221 tctgtatttt gttcccaacc atctattttc ttttccacag tagctgcttt tcctttttca
    50281 ttcgcgacaa catacgaatc ttgaagaaga aggcaacttt ctcttacagt ataataacct
    50341 tgagagtcta ctggattatt gaaatcacct aggaaaaaaa gtggtacgcg agtttccaaa
    50401 aagtagtttt ccaagttttt ccactcaaaa taaaagccac tttttttatc tgtccaccaa
    50461 gaaaagtggc agctcgagac aaaaatgtcc tgatcatcta tctttgtttt cccaatcaag
    50521 atcttgcgtg tctgataatc ttctttcttt tgaaattggg agacggtaag gacctgactt
    50581 tccaatgttt ttttcgtaag cagtgcattt ccttcttcat atttatcata accgatgtgg
    50641 ctcatttccc aactccaaaa atactctttt ccgtgttcct ttaaaagctg aactaaacgg
    50701 taggcaaaat tatctttacg gataggtact tgattcttta ccggacagaa cttcattagc
    50761 ttcgcaggtt ctacttcttc agattccagt aattggttga tttcttgtaa agcaatcact
    50821 tcataatctt ctaacaggat cttatctgct aattcttgca attttttctc tggttctggc
    50881 tcaagccagc tatgcgtgtt gagtgtcatg aatttcattg gttttctcct tgtcttcatt
    50941 tcgaaacaaa agggttgtga caaaattttt gtcacaacct ctccagcaaa tagtgttctc
    51001 ctgctaactc ctcagtaata agctcaatgg accaggagct attttttgtt tggctgtttt
    51061 tatcacttgg tgcagggcag ctttttcacg acctctccac taaacagtat tcaaactgct
    51121 gcttctcggt aataagccca accaaacgaa aaataggagg agctattttt gtttggctgt
    51181 tcttatgact tgatgcagga cagctttttc acaacctctc cttacatttt aatggagccg
    51241 atcactttac cagctgtcac tgtacctaat tgcttgatca ctacttcttc tactttatcg
    51301 tttgtaaata cgatcatgat cgttggatct ttttcttcgt tttggatcac atcaagatcc
    51361 atctcagcta atttcgttcc tgttcctact tcttggcctt ctttgacagc aatctcaaaa
    51421 gctggctgat tcatttggac tgtatcgatt cccatatgga tcaaaacttc gataccatct
    51481 ttcgtttcca gcccaattgc atgtttgcta gggaaaatac ttgtcacttt tccagcgatc
    51541 ggcgcaacaa cttctcttga tgttggaata acagcaaagc cgtcacccat cattttttgt
    51601 gagaataccg gatcttttac ttcagataaa ggaatcactt ctccagaagc tgcggaaact
    51661 agttcttgtt ctatacctaa gtattgctta ctacctgctt gtccatttcc tgtagaaacc
    51721 atttctggct ctggaatctc tgctcctgat tgcagcagat cttcgatatc tgatttcaag
    51781 acatctgctt ttggtccata aactgcttgg actccattgt ctttgacgat caatcccatc
    51841 gcccctgctc gtttccaggc ttcttcagtc cctacttttt ctcgatcatt tacacttacg
    51901 cgtaaccgag tcatacaagc atcgacatct ttgatatttt cttttcctcc cagcaaatga
    51961 atgatctgga ccacttgctg atctgctgtt cctgttccgc tgttagatac aacggtttct
    52021 tcactgtcat tgtcgtaatt tccgttacgt cccggtgtag caaagttgaa ttttttgatc
    52081 aagaaatttg ctaagaaata cgtaacaaca ccaaaagcga tcacacacaa cacgaagttg
    52141 ataagatctc cacctagacc cgctttgata gctaacggtg tccgagtcag caattcgata
    52201 ttcccaaatg aatggacccg caatggcaag atatctgcca tcgcaaatgc agccccttgg
    52261 atcactgaat aaatgacata taatggcaca gcagcaaaca taaacatgaa ctccaatggt
    52321 tctgttacac ctgttagaaa gacagccaac gctgctgaaa aatacattga tttatatctt
    52381 gctttcttat caggatcgac attacgatac atcgcaaaag ccattcccat caagatacct
    52441 gaagaaccaa tcatttgtcc tactttgaaa cgagccggcg tccagttatg caacacaaac
    52501 tcatattgcg acatatcccc tgcaccttta agattcacta aatcagtggc ccaagccaac
    52561 cataacggat cttgcccaaa tacttgtgtc cccgcctgtg cgcctgataa aatctcatac
    52621 gatccgccaa gctgtgtata gttgatagga atcgtcagca tatgatgcaa tccaaatggc
    52681 aatagtaaac gttctaatgt tccaaataaa aacggtgcta gaatcggtgc gctttcttgt
    52741 gattctgcaa tccataaacc aaaattattg attccagcct gtacgtttgg ccacaccaca
    52801 gcaagaatca atgccacgat tgttgaccat aagataacaa caaacggaac aaagcgcttt
    52861 ccattgaaaa atgacaaagc atccggcaat tttctataat tatagtactt attataagcc
    52921 attgccccga caaatccagc gatgatccct acgaatactc ccatattcaa agctggtgct
    52981 tccaatacac ttgtaaaaaa tcctttaacc atgatcttcg taccgaacag agtgtgggta
    53041 aatgcatctt cattcgcaag catgtcactt gtgacaccaa agatagcccc agtgatccga
    53101 ttgattaaaa taaatgagat cccagctgca aatgctccgc ctgctctttc ttttgcccaa
    53161 cttcctccta ttgctaaagc aaataaaata tgaagattac caataattgc ccaaccaata
    53221 ttttcaatta ctccgcccgt tgtgacaagc ggcgtccaat caggattgat caagggaatt
    53281 gatttcccta tactgatcat caacccagct gccggcataa ctgctaccac aaccattaaa
    53341 gcctttccaa atttttgcca aaactcaaaa ctaaatatct ttttcattcc gtttagctcc
    53401 tttacgcaat cggtttcgtt aaacgtatta tagcgtgcgt tttctttttt gtaaatgctt
    53461 tattttttat ttttctgtct ataccattct ataaaaccta taatacgtgt gttcccttct
    53521 ataattttct cctaaaaatt tattttttta agcttttttc tatgaaatat tttcttttgt
    53581 ctattgactt gtgcaatcgg ttgcgtttat tatgaacgta taaaacagct cattaggagg
    53641 agaaaaatga aacagatcaa acgactattt gctattgatc catggaaaat cagcacgaca
    53701 caattagata aaaaaaatct tcgactgcaa gaatctttaa caagtattgg aaacggttat
    53761 atggggatgc gcgggaactt tgaagaaact tactcagggg accaccatca aggtacttat
    53821 ttggcgggtg tctggtatcc cgataagaca cgtgtcggct ggtggaaaaa tggttatcca
    53881 gaatactttg gaaaagtgat caacgccttg aactttatcg cgatagatat ttcgatcaac
    53941 gatcagctga tcgacttggc ttctttggaa ccagaagact tttactggga attagatatg
    54001 aaaaatggtg tattatctcg tacattcaca atcactactt ctactaataa agtacgcatt
    54061 tccttcgaac gttttctgag cattgtcaaa aaagaatcag cttatatcta tttaactata
    54121 gaaatgcttg aaggtagcgg aacagtcaaa ctcgtatcaa aattggatgg aaatgttcaa
    54181 aacgaagaca gcaactatga tgaacatttt tggaatgaga ttgatcgtgg aactatagaa
    54241 gaaatcggct atttgacgac taaaactatt ccaaacaact ttgggattga acaatttact
    54301 gttactgcag caatgcgcca tttcgttaat ggaaaatcag tcatccctga ctatcacgaa
    54361 gatactttag cattacatgg ttgcttgtct gcagaattat ctcaaggtga gacatggtca
    54421 gtggaaaaag aaattatcgt tcttactagt agagatattc cagaagcgca gcaagcagaa
    54481 aaagctgtct cagaattaac gaaccagaca aacaattata tccaagcaaa agaagagcaa
    54541 acagcggctt gggcagaacg atggaagctt gccgatgtgg tcatcgaagg agacgaagaa
    54601 gcacaacaag gtattcgatt caatttgttc caactattct ctacttacta cggcgaagat
    54661 gaacgattga atatcggccc caaaggattc acaggagaaa aatacggcgg agccacttac
    54721 tgggatacag aagcgtatgc tgtccctctt tatcttgctt tagccaaacc tgaagtaact
    54781 aagaatctgc tgaaatatcg gcataatcaa ctgccgcaag caatccacaa cgctcagcaa
    54841 caaggattaa aaggcgcgct ttatcctatg gtcaccttca ctggtgtcga atgccataac
    54901 gagtgggaaa tcacattcga ggagattcac cgaaatggcg cgattgccta tgcaatctac
    54961 aattatacga attacaccgg cgatacggaa tatttgaaaa atgaaggact ggaagtttta
    55021 gcagaaatcg cacgcttctg ggcagaccgt gtccattttt caaaacgagc aaataaatac
    55081 atgatccacg gtgtgaccgg cccaaacgaa tatgagaaca atatcaataa caattggtat
    55141 acaaatacga tcgcagcttg ggtcttgcgt tacacgcgag aaaattattt aacttatcaa
    55201 aaagaagctg ctatcactat cagcgaagaa gaactagcca aatggaaaga tatcgcagag
    55261 aacatgtact tcccagttga cgaagaatta ggaatctttg tccagcatga cacattctta
    55321 gataaagatc tcatgcctgt atccgatatt cccacaagcg atcttcctct gaatcagcat
    55381 tggtcttggg acaaaatcct tagatcttgc ttcatcaagc aggcagatgt actacagggg
    55441 atttatttct tcaatgatac ttttacaaaa gaagaaaagc aaaaaaactt tgatttctat
    55501 gaaccaatga ctgtccatga gtcttccctt tcgccaagta tccattcgat tttagcagca
    55561 gaattaggaa tggaaagcaa agctgtagaa atgtaccagc gaacagctcg cttggatttg
    55621 gataattaca ataacgacac agaagacggc ttgcacatca cttccatgac tggtagctgg
    55681 ttgtcgattg tccaaggatt tgctcaaatg aaaactgccc atgatcagtt gagattcgca
    55741 ccatttctgc caaaaacttg gaaagcttat tctttccata tcaattatcg cggacgttta
    55801 ttgttcatct cagtcacaga tgaacaagtt actttcgagt tattagaagg ttctcctcta
    55861 gaaatttacg tctatgaaca gtcttattct ttagcggatc gtctagaaat aacgctaaga
    55921 aaggaggaag caaatgttta aaggggtatt gtttgattta gacggtgtca ttaccgatac
    55981 tgctgaattc cattatcgtg cttggcgaaa gcttggtcag gaaatcggca ttacgatcga
    56041 ccacacattc aacgaacagc taaaaggcgt cagccgagaa gactcactct ctttgctttt
    56101 agcttatggc ggaaaagaac actctttttc aaaggaagaa tttgcagaat tagcgaaaag
    56161 aaaaaacgat tattatttag agatgatcca gacgatcgaa ccaaaagatg tcttccccgg
    56221 ggtcgtgcct ttactggatt ctttaaaaga agaaaaaatc aagattgctc ttgcttctgc
    56281 aagtaaaaac gggccattcc tacttgaaaa aatgggtctt acaccttact ttgatgctat
    56341 tgccgacccg gcagaagcaa caaacggaaa acctgcgcca gatatcttcc tccttgcagc
    56401 aaaagctgtc ggactaaaag cagaagaatg tatcgggatc gaagacgcgc aagccggtat
    56461 ccaagccatc ctctcaagtg gcgctcaacc aattggcgtg ggacgtgctg aagaccttgg
    56521 agaggagatc ccgcttgtcc ctgatacgac ttttcttacg atcgattact tgaaagaaaa
    56581 gtggcatgac catggataat ccagagatct ccgtcgtacc agttccagag actgaaataa
    56641 agaaaatcac attgaagaac gactacttag aagtcgttct tctcaattac ggtgcccgcc
    56701 tgcaccaaat cttcgcacca gacaaagaag gaaaatcaga aaatgtttta ttgtcttacg
    56761 atcgtttcga agatgttcta acagacaaaa gcttttttgg agcaactgtc ggacctgtag
    56821 ccggaaggat ccgggatgct tcttggggtg agcatagatt ggagaaaaac tgcggatcac
    56881 accatatcca cggtggcacg aaaggctggt ctttccaatt ttgggaagta gaagttttca
    56941 aaactccatc atcaataggt gtggtctttt acttaaaaga tgaattttct acctatccag
    57001 gaccaatcac tgctacgatc acttaccgtt taacaaaaaa cgaattagag atgacaacct
    57061 ctgctagtag ccttgttgaa accatctgca atccgaccaa ccatgcctac ttcaaccttt
    57121 caggaaatgg aacaagagac atctatgaac atcagttaac ggtcttttta gatggtgtgc
    57181 ttgaattaga tcaagaaaaa ctgcctactg gaaattggac aaaaaaagaa aaactgccaa
    57241 tcgactttag aaaatcccca acactgcaag agatccttgc atgttatcca gatggactag
    57301 atgatgtatt tcttttgcat catcctaggc tttccaaaac ttccttacaa ttattcgaga
    57361 agcattctgg tcgccaaatg actatagcaa cttctaataa aagtatggta ttattttcta
    57421 cgactggatt cgaagctgat ttctctgtca atggaaaaca gatgcattca aactacggat
    57481 tagcaatcga accgcaagaa atccctgata tcgttcattt ccccaaattt ggatcgatca
    57541 acctccatcc tggtcaagaa cggatcagtc agacgattta tcggttctct gcacaataac
    57601 aagagaaagg tgtttttatc atggctatta ccgtaaaaga tgtggcgaaa aaagccggtg
    57661 ttgccacctc gaccgtctct cgtgtcatca acgatcatcc tagtatttcc gagacaacaa
    57721 agaagaaagt ccgaaaaatt atggatgaac taggttatgt tcctaacatg actgcccgca
    57781 atctaggaaa acgcatttcc agtgccatcg gcgtaatctt accccctctt gattcaaaag
    57841 agcggatcgg taacccattt tatcttgaga tcatggaagc aatcaacgaa gaagcgcgga
    57901 attatgatat gactacagca atcgctactg ctaaaaattt tgaattgctg ttagagaacg
    57961 tccagcgtat gcatttacaa aaacaagtag atggttttat tctagtgtat tcagatagtc
    58021 aagatactgt cattgattat ttataccaaa accacatccc tttcactttg atcggacagc
    58081 cttatcatca tgagaacgaa atcgtttatg tagataatga taaccagctt ctaggtaaac
    58141 aagcaacaga atttttgatc cagaatggac ataagaatat cctgtttgtg acaaacacga
    58201 ctcatgaaag tttgtatttc gaacgatatt tcggttatca aaaagcaatg atgatggctg
    58261 gtcttcctgc gttatccagt gtagtactag agcatccgga ggattatctg tcctttgaga
    58321 aaacgttgaa agaaaacgaa gccacagcat tggtggtcat tgacgatatc ttcgcccttc
    58381 gcacgatgca gttggcgcaa atgtatggct atgtcattcc agagacactc tccgtcatta
    58441 gcttcaacaa ctcgatcttc tcaacattaa cacaccctta ccttacaagt atcgatatcg
    58501 atatctccga attaggacgt gtcgctatgc aaaagctgcg cgaattgatc catgaagaat
    58561 tgagcagtgg cgtacgtttg gtcatccctc atcgcttgat caaaagagag acagtcgttc
    58621 ctttagaaaa cccatactaa atagctgact cactaaatac gctgaccatt tatcctatgg
    58681 tcagcttttt atgtaaaaga agatactact tctaacacat tgtttatctg taaaaaagag
    58741 cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa
    58801 gcagaagcaa ctccttcgga aataagctga aattcacaaa aatttaaaaa gcaattttct
    58861 tgagtttctt cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttccttcct
    58921 tcaatttttt acacgtcgac gattcaataa aaaaccgcct aatattgtaa agacaggtgc
    58981 aagaatcgag aaaaaggcga atggcaagta atgcaacgcg ctaactttca tcgttcccat
    59041 aatgaatgtg ccgctgactc cccacggtac gagagaattg acggtcgcac ctgaatccgc
    59101 tagtgttcgt gtcaaatagg tttttgataa gccaacgtca tcaaaagaac gcttgaatgt
    59161 ttctcctgga agaatgatgg acagatactg ttctccaacg atcagattga ttccgataca
    59221 gctcaaggca gtaaacccga ctaaacgaga aggtcggtcc atcttttctt ttaattcttg
    59281 aacgatggta tcaacgatca gatatttgat cagtaaccca cctaatgcca atgcaagtaa
    59341 aatcaaacta gcagaaccta acatactcat gatcccgcct cgagaaagca gttcatctag
    59401 ctttggatca ggtgtatttg cgacataacc attcatcaag atgtctgctg cttttccgac
    59461 cgtcaaactt ttctcatgga taaaagaaag aacgagagca gctgtcgatc ccgctagtaa
    59521 tgtagggata gccggaattt ttttgattgc ccccccaaat aaaataacta gtggaatcaa
    59581 tgtccaagga gagatccaaa agctcgcttt catgttttgg atcatttcat tgatactagc
    59641 tagatcggct gaatgactgt tatgtcctaa tatcgtataa aaaatcaacg aaaggacgaa
    59701 tgctgggaaa tccgtataac gcataccagc gatatgttcg aatagattaa ctcctccaat
    59761 acccgctgcc aagttggtcg tatctgataa aggcgagcag ttattcccta aaaaggcacc
    59821 ggaaacgact gcgcctgtag tcattgcatc attgaatcca agtaaatgac cgatccccaa
    59881 aaaagcaatt ccaatcgtcg aaatcgttgt aaatgaactg ccgacagtca ctccgacaat
    59941 tgcacaaaca agaaatgctg tcggtaaaaa gaaacgcaca gaaacaagtt taaatccata
    60001 aaccataatc gtagggatgg ttccagataa aatccatgaa gcaactaaga caccaatcaa
    60061 aagaaaaatc aggattggaa tgatccctgg agtgattcct ttgatgatcc cgtcatggat
    60121 ctcatcccat gtgaactttt taaatctgcc ataaaacaac aaacacatga aagctgcaag
    60181 gatcgggata tgtggcgaga gctggaatcc gatgatcagt actcctagga gtgccaacaa
    60241 cacgatcaat acagcgatac tttcttttaa agaaatattt ttattcatcg tcgatctcct
    60301 atttcaaatg aaaaggccct gccatgaaaa aactattcac ttggcaggac ctcctactaa
    60361 atcagcgaca aaacgtcggc tgaaagactt atttttttat ttttcttcta aacttttttc
    60421 tacctcaatc ccccaagaat ctttcttgaa gtgattgatg atcaatggaa taacgaacat
    60481 gaccactgtg atagctgcta ctaataatgc atcactcaat gtcgctgtac ctaatgtagc
    60541 aaatacgaca acgatgatag agaagatcag catgcagctg actacccaag ctaatccgtt
    60601 tccgttttta ccaattcggt aaggacgttc gaattctgga tgtttcttac gcattttaag
    60661 caatgaaaca gcaatcaaaa tgtacacgat acaataaata acagttgtag catttgtaag
    60721 tgtcaagaat acagcactta cgtcatccat tacaccataa agcaaagcaa ataatgaaat
    60781 aacgattgat tgtgttagaa cgacatttct agatacgccg tatttgtttt ctttatggaa
    60841 tccgaatttt ggtggaagga atccttcacg agctacttgg atgattgttt ttgatggtcc
    60901 agtaacccaa gctgataatt gtaaaagcac accaataaat accatgaagc tgaagatatt
    60961 tccgatcact tctggtaagc ctaggatttg acagtcaatc aagataggct gcgtgatatt
    61021 tgctaattcc atttttccat ctggtacgac atttgctaca agcatcgcat tgatgacatt
    61081 caataaaact aatccgatca aagcgataaa tacgcctttt gtataatttt ttgttgcgtc
    61141 tttcaaacga ggcatgtaaa cagaactcat ttcgatccca acaaaaataa atgtgatacc
    61201 agctaagtat ttcaatgaat caatatgttc caagtcaggg aaagcttttg accatgaaaa
    61261 gtcacctaga tagccgtttg agactagtcc gactttgaag gctgccaaaa cacctaaaac
    61321 gaacatgatg actactggga tatatacccc tagccataca ccgatgttcc cgccgacttt
    61381 tgccatatcg aatttcaagt tcaagatcgt gatgatccag tagataacta aaatacaacc
    61441 taaaacgaac cagtgattat tgccaagttc tacattacca aatgtgtttc ctaacaatgg
    61501 tcctaaagta gaagcgacca ttaccatacc tgggaacatt tgcacccata atagccatgc
    61561 aacgacaaat ccccatttgc tgcccagtgc tgtttttacc cataactgag gaccgccttc
    61621 ttgaggaagc atcgtggaaa gctcaccgga aaccattgat atcggaccgg caaaaaacaa
    61681 aactgcaaaa attgaataaa aaatcaatgt ccaaccgact gcggctaacg taggaatact
    61741 ccgaaccgtt gcacacagtg ccattgtcag accgataaag gtcccaagac ccattttact
    61801 tacttcagat ttactcaccg ttgacactct ccttgatttt ttggataaaa taacggtgtc
    61861 tttgaagtag tttcagcaat aaacgaaaca attcagcgaa tatttgaggg gattctttga
    61921 gtttattgct tgaagggttt cttcaaagac aacctcaaaa caaagggatc gaagagctta
    61981 ggtctcccct tgctcttcag atccaaacat gcgtcagaaa cagttagtat tcaagctgtt
    62041 ttctaacatg attaccttgc ttattttgct tcgcttgcca ataattggtc tgcgtagatt
    62101 ttttccaatt tttcttgaag agctgctttg attttttcag catattcttc aaagtgttct
    62161 tctttgttca tgtatggtgt cattacgctg gcacgcaata cagttacttt tccggcacgg
    62221 ttccattctt catctgagaa tcctagttgg ttcacaaatt gtaatgggct gttgccatag
    62281 tctgggatag caaagtcagt atgtgaagtt aagaactcat taccgtaaat gcttcctttc
    62341 acgtatgaag aataatcata tacgtcatgg ttcaatttat tcattgcaac taagtcatcg
    62401 ttgccttttt ctttgaatac atagtctacc atattgaaat ctggataagt taatggatga
    62461 acttcgattt ctttgtctcc tactttaaat gacaagtcat tcaagaagtt gtagaaacga
    62521 tgtgcacctt cgattgatgc acccattaat ttaccatatc ctgtgacatt caaaggtaag
    62581 acatggtgtg ctgcccaaac gctggctgct gttgcccctg cttttgaacc ttccaagata
    62641 taagccccaa gtaatgctgg gatatctgcg cctttttcaa atacatatgt cgcaaagtaa
    62701 gagataacgt ctctcatgcg gatatctttg atgacgatac caccggcaga atatggaaca
    62761 tagcccattt tatgtgggtc aatcgttaca gattctgctt cttcgattgc tttatatgca
    62821 ctgtgtactt cttctaagat ataatctttg ttttctgtaa agacattgta tttgtaatga
    62881 acatctttta aatcttcaaa tggaatgaag ttgttgtctt cgtctaagaa aatcgcacgg
    62941 ccgtatccgc cataagcagc atctacgtgt aagtagaagt aaataccgtc tttttctaat
    63001 acgcgacgca atgcaacgat tttatcgata ccgtcgattg ctccttcttc tgttgatcct
    63061 acaacaccga ctacacctag gattggtgtt ttttcagctg ctagaccacg aacgattttt
    63121 tcaagttcgt tgatgtccat gcgatagttg tggtcaactg gcactggaat cacttggtct
    63181 aaaccgatac cgatgatgtc ggcagctttt agccatgaat agtgtttagt ttgtggaacc
    63241 aaccattttc ctaatttttc taaatgtttt ccgctacgtg ctgagtgagc tttgatttcg
    63301 tcgatttttt ctgggacgct gtctaacaga ttcatgattt cttctgttga caagttcatt
    63361 aattcccaat cgctttttcc agcaactagt tcaggtgtca cttctttcat tgcaagcggc
    63421 aatgatttga tgttgcgggc ataccaaagt ccttctaagt ttgctaaaga accatcagca
    63481 acgatgtggc cccagccatc tttatagctc attaattttg caaattccat tcctacttct
    63541 tcttccattt ggcttgttgc aggagaagat tcataggcaa cgttattacc attccatagc
    63601 atagcaaaat tgtacgctag taaagatggc atcaatgttt cagagttcat atgaccccag
    63661 taacgtccgg cattatgcca aggaacggaa tgggtacgca tccgcgctga aatttctgat
    63721 agaacatcct ttgttttatt cacagtgtgt tcaaaactag cactgctttt ttcttctggc
    63781 gtaataatag gcatgtcttg aggcatgtag ttttgacgcc agcctaaatg ttcatccacc
    63841 aattcattca acaaagcttt atagatttgg ccgttctcag ctttgtcccc aatgaatagg
    63901 gcatttaggt ttagatcttt cgacaatgat tcactcatag tgaaaccctc ctaaaaaaat
    63961 atttaaattt tgtcgagccc tacattacac ctgttcttct attctgtaaa ctaagttttg
    64021 gcctgatttt taatcatatt ttaatatgta tcaattatcc gaattgttca cacttttgaa
    64081 ataattaaaa aaaacgaggg aatttacaaa tgtaaactcc ctcgcaaaga ttgtattaac
    64141 gacaaccagc gttctatttt aggatgggtt tcaaaattgg aatgttgtaa tagtatttcc
    64201 cttttttaag gtaaaaactt actaaattct aatcaattac tttggccaaa aagtaatttt
    64261 tcttaccttt tcgaataacc acaaacttac catcgaattc atcggatgga tttatgacaa
    64321 aatttaaatc ggtaacgcgg tcaccgttaa tacttatagc tccgtttgaa acatcttcac
    64381 gtgcttgacg tttagatggt tcaatttttg tggaaacaag cagttccacg atattttcag
    64441 gtgtgcttga aatttcaacg ttcggcattt taccgaatcc ttgcgcgatt tcttccgcat
    64501 ttagttcttt aatatttcca gagaacaatg cttgcgttat tttttgcgct tctttcaagt
    64561 cttcttcact atgcacaaat cttgtaactt cttctgccaa acggcgttgt gcttcacgtt
    64621 tctcaggttc tgtctctact tttttcgcta agtcttcgat ttcttcttga gacaagaaag
    64681 taaagaattt caagtatttc actacatcgc gatcatcttg attcaaccag aattggtaga
    64741 actcaaatgg tgaagttttc ttcggatcaa gccaaacggc accgcctgca gtttttccga
    64801 atttcgttcc gtcagccttc agcatcaatg ggatcgttaa accgaacgct tctgcttctg
    64861 gtccttctaa ttttcggatt aggtctaatc cagctgtgat atttccccat tggtctgctc
    64921 caccgatttg caattgcaca tcgtatgttt tatgcagatg caaaaagtcg atagattgca
    64981 ggatttggta agtaaactct gtaaatgaga ttcctacttc caaacggctg gcaacgatat
    65041 cttttgctaa catcgtgttg atgttgaagt ttttaccgta gtctctcaaa aattcaagta
    65101 aagagatttt tgacaaccag tcgtagttgt tcacaaatgt gatatttgca tctttaccaa
    65161 acaattttct catttgattg gaaagtgcgt ccacgttatg ttgcacttgt tccatcgttt
    65221 gcaacacacg ttctgttttg cggccgcttg ggtcgccgat cgttcctgtt ccgccgccaa
    65281 tcagaatata tggatgatgg cctgccaatt ggaatctttt catcatcata aatggaatca
    65341 aatgtccgat atgcatcgaa tcaccagttg gatcaacgcc gcaataaagt gaaatgctct
    65401 tttcttcaac gagctcgcga agtctttctt cgtttgtttg ctgattaatg gcgtcgcgcc
    65461 aagttaattc atcaataata ttcattttac tgacctccgt gtagtttttt tatgaaagca
    65521 ggaacctgct agcatatgta attaattatt tcttcaggat aggataggaa ataaaaaaag
    65581 tcccttggca tataagccaa gggacgaaat ctcgcggtac cacccaattt gtatgtctag
    65641 atatccaaca tacctctcga acatttaacg ctgtcgcgtg gcaatcactg ccaaagactc
    65701 ctgaaatgta attcgcttta aagcatgtac tggtttgcag cgaccaccag ctttctgaaa
    65761 cattggcttt aacaactact tcgtttcgat cttcgtcttg ttattattaa tatgtgtcta
    65821 tcatagagcg caactttttc tttgtcaaca gaaaaaggat atttttttta aattcctcat
    65881 tttttctttt ttgaatccgc tctaattccg aacattttat tttcaattat tgttaaaaga
    65941 aataactttt tctgtatttt ttctgcaatt tatcgagaag tcactttact ttgcctctta
    66001 ttctttcttc gttacaataa ggaatgggac aagtaaaaaa atgaaaaagc aagggacgag
    66061 gtatatggaa aaaaagcaaa aaattatttt aggagccact tctgctttaa ctatcgttat
    66121 tttaattcta agtttgtttt ttgtcatccg atcatctaca aaaaaagaac caatcattcc
    66181 tgaaaaaaaa ataacagaaa aagcaaaaga accgacagat gataagcaac aactagttac
    66241 tcaagcaaaa aatctggctc aaagctacga ttatgaccaa gcgattgcgt tattggaaaa
    66301 agatagtaag aacggatcta ctgaaacaca acagcttctt caaacactaa aaaaggagaa
    66361 agaacaactg atcgactggt ctgatcctac tcagatctct catgtctttt tccacagctt
    66421 gatcgtggac ccgcaaaaag ccttccactc tcaacaggca caaggataca aagattatat
    66481 ggtgactgtt gaagaattca accgttcgat cgaacaactt tatcaaaatg attttgtttt
    66541 agttaatttg aatgatttga tccaaaaaga cgaacaagga aatctttcct ttaacgggct
    66601 tgcgctacct gaaggaaaaa aaccattggt tctttcacaa gatgatgtca gctattatga
    66661 atatatggat aattccggtt ttcctagccg attggtcgta tcaaataaca atgaaatcaa
    66721 aaatgtctat aaagagaaag ataaagaaga aatcggtgac tttgacatgg ttcctctgat
    66781 cgatgccttt gtcaagaaac atcctgattt ttcttatcga ggcgcaaaag gtaccttggc
    66841 cctgacaggt tacaacggag ttttaggtta tcgaacatca aaatctcaat atggagacaa
    66901 tgaaaaaaca aaaaaagaga ttaaagaagc taaaaaagtg gcggaacagt tgaagaaaga
    66961 cggctggagc tttgcttccc atacatgggg tcacttgaat atgactcaag cttcactaga
    67021 agatgtaaaa aaagacaatg aattgtggca gcaagaagtt gcacctatcc taggaaaaac
    67081 aaatattctg atttatccat ttggtgcaga catcagcgac tggcagcctt attcagcaga
    67141 aaatcaaaaa tttgcttact tgaagagtca aggtttcgat attttctgta atgtcgatgc
    67201 ttctacacca gcttggggac aattaggtac ggatttctat cgaaatgctc ggatcaatat
    67261 cgatggcatt cgttttcaat ctgatttaga cggaaaaaat cctattcttg atccattcat
    67321 caatgtccat gatgtctatg acaaagaagc acgcgaatct gcttgatttt acgtcattta
    67381 tgaaccaaaa aagccgaact gacattttat cgtcagtttc ggctttttta tgctttattt
    67441 cttcttcggt gcgattccct tgatcaggac ttctgtccat tctgctgtat atggaagcag
    67501 ttttgcgtat atctccggct tagaaattcc ttctaccatt gcttgaaaat agaggatcaa
    67561 gaactcatcc gaatatttca aatccacttt tccttctttc cgccctcgat ggaaaagatc
    67621 aagcatgata ctgaaacttt cttgcgtata cgcatccatc gcgtcagaaa attctttttt
    67681 ttcttttttt gtatagtatg tcatcaaatc gatataaaat tgtttgttca ctcgattgag
    67741 ataacggatt ttgttttcac tcatggcaat cagtgtttct tcaaatggaa gattttccgc
    67801 aatcaacgct ttcgcttctt gccctaaatc attcataaat gcaaaaaata cttcataaat
    67861 cagcccttcc ttgctgccaa aatatttaaa aatcgtcgct ttacttacct gagcgttctc
    67921 agctacctta tccattgtca ctccagcggt tcctatgtct gtgttcatca agtcaaaagc
    67981 tgcttgtaaa atctctttct ttttctcttg tgctctcttt tcaaaaccgt tcatttgtag
    68041 agcctcccgt aattttaaac ttttataata tgtatagttc aaatttattc tgaggtttat
    68101 tataactatc tattgagaaa gtatcaagct agcaattcct tgtttatgaa ttcattgaca
    68161 gaaatttata aaaagaatat tatgtactaa aataataaaa aaagttttat aaaaaggtat
    68221 aaaacctttt tattaaacta tttaatcttg tttagtatat atttttagaa aaaggagtgg
    68281 ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg gtcgtttcga
    68341 agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat ttatcggacc
    68401 aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa aaaaagacgg
    68461 cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta tccatgaacg
    68521 tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag gagcgatcat
    68581 cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg aactgattca
    68641 acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa atcgccaaaa
    68701 agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg atgagcctac
    68761 ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa aattgaaaga
    68821 acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc gactagcgga
    68881 taaagtcgtg atcatacgcg aaggaacact cgatgaagaa ggaacactcg atgaattgcg
    68941 tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc acttagcaca
    69001 attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg ctgctgataa
    69061 cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa aatttgaatc
    69121 tattccaccg actcttgagg atttattcat gagacattat gaaggatgag gaggaagcga
    69181 aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta gccgtgactg
    69241 gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt ttgtccctgc
    69301 gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct tgcaaaaccc
    69361 ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt atactttagg
    69421 agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg tgatctctgt
    69481 tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg aacttattcg
    69541 ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa gtattctgat
    69601 caatttgctt ttagcaatat cgatcagctg tacgatgatt tttttccaag cggattcgat
    69661 cactgcagaa ggtgcttggc tttttgggct atctgtaggt agtatgggga ttttggggag
    69721 tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga atggtctgtc
    69781 tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag ctgattcctc
    69841 cctttcgatg tggaatcctt taggctggtc ttatttgacc tatccattta caactaacaa
    69901 ctggattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca gttcatttgt
    69961 tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag gaaaaccgta
    70021 tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata aagggatgac
    70081 aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta tctacgggaa
    70141 tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc aatctggcgt
    70201 ctcaattgaa aaaaacttta caagcacgat catggtagtc ttgatttgtc tagctgctat
    70261 tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac gtatgaatca
    70321 gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt tactagctat
    70381 tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt ctgctttggc
    70441 ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg ggttcaattt
    70501 cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat ggcttcctcg
    70561 aatcggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt actttggcgg
    70621 aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg ttccacggat
    70681 gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca gccttgcact
    70741 gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat aataaaaaag
    70801 catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt tggaggtcat
    70861 ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc tcgattcttt
    70921 ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt ttcgggggtc
    70981 gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg atccttccct
    71041 gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt ttccatcatc
    71101 aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc taaaagtttc
    71161 agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct caatatgcaa
    71221 atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc tagcaactgg
    71281 ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt tccgctagca
    71341 gataattgat acactttcat actgccggag gccacgatca ctaattgagg ttctccattc
    71401 ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc gtgtactttt
    71461 tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca taaatgatca
    71521 tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca taacttccct
    71581 tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc agataccagc
    71641 aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag accttaagta
    71701 attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac aaaccttcac
    71761 tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt ctattgaaat
    71821 ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc agcggttatt
    71881 tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg aaaggttcgg
    71941 tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata gtcagacaaa
    72001 gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc cttgatatcg
    72061 actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc aatgctacca
    72121 tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg tttttgattc
    72181 aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta cgcgctccaa
    72241 aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt gctcatgccg
    72301 tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt ttgccagtgt
    72361 tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc tttgagctgt
    72421 ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag ttccattgtt
    72481 tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga caaatagagg
    72541 ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa ctgcgatgag
    72601 catcaccgca gtttta
//
LOCUS       DAZKCZ010000002        63878 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000002 DAZKCZ010000000
VERSION     DAZKCZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.002"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..612)
                     /gene="dhaL"
                     /locus_tag="ACG8KN_000062"
     CDS             complement(7..612)
                     /gene="dhaL"
                     /locus_tag="ACG8KN_000062"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9187162.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(640..1650)
                     /gene="dhaK"
                     /locus_tag="ACG8KN_000063"
     CDS             complement(640..1650)
                     /gene="dhaK"
                     /locus_tag="ACG8KN_000063"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9187163.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1625..2020)
                     /gene="dhaM"
                     /locus_tag="ACG8KN_000064"
     CDS             complement(1625..2020)
                     /gene="dhaM"
                     /locus_tag="ACG8KN_000064"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9187164.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2174..2494
                     /locus_tag="ACG8KN_000065"
     CDS             2174..2494
                     /locus_tag="ACG8KN_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9187165.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2606..3463)
                     /locus_tag="ACG8KN_000066"
     CDS             complement(2606..3463)
                     /locus_tag="ACG8KN_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9187166.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3571..4875)
                     /locus_tag="ACG8KN_000067"
     CDS             complement(3571..4875)
                     /locus_tag="ACG8KN_000067"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9187167.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4872..5381)
                     /locus_tag="ACG8KN_000068"
     CDS             complement(4872..5381)
                     /locus_tag="ACG8KN_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187168.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5556..7691)
                     /locus_tag="ACG8KN_000069"
     CDS             complement(5556..7691)
                     /locus_tag="ACG8KN_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9187169.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7774..9429)
                     /locus_tag="ACG8KN_000070"
     CDS             complement(7774..9429)
                     /locus_tag="ACG8KN_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9187170.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9431..9745)
                     /locus_tag="ACG8KN_000071"
     CDS             complement(9431..9745)
                     /locus_tag="ACG8KN_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9187171.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9915..10175
                     /locus_tag="ACG8KN_000072"
     CDS             9915..10175
                     /locus_tag="ACG8KN_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFP9187172.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10189..10503
                     /locus_tag="ACG8KN_000073"
     CDS             10189..10503
                     /locus_tag="ACG8KN_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFP9187173.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11233..11787)
                     /locus_tag="ACG8KN_000074"
     CDS             complement(11233..11787)
                     /locus_tag="ACG8KN_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9187174.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11913..13842)
                     /locus_tag="ACG8KN_000075"
                     /pseudo
     CDS             complement(11913..13842)
                     /locus_tag="ACG8KN_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14148..14864)
                     /locus_tag="ACG8KN_000076"
     CDS             complement(14148..14864)
                     /locus_tag="ACG8KN_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9187175.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14865..16688)
                     /gene="glpO"
                     /locus_tag="ACG8KN_000077"
     CDS             complement(14865..16688)
                     /gene="glpO"
                     /locus_tag="ACG8KN_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFP9187176.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16702..18198)
                     /gene="glpK"
                     /locus_tag="ACG8KN_000078"
     CDS             complement(16702..18198)
                     /gene="glpK"
                     /locus_tag="ACG8KN_000078"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9187177.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18334..19794)
                     /locus_tag="ACG8KN_000079"
     CDS             complement(18334..19794)
                     /locus_tag="ACG8KN_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9187178.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19784..21079)
                     /locus_tag="ACG8KN_000080"
     CDS             complement(19784..21079)
                     /locus_tag="ACG8KN_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9187179.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21411..22088)
                     /locus_tag="ACG8KN_000081"
     CDS             complement(21411..22088)
                     /locus_tag="ACG8KN_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFP9187180.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22118..23209)
                     /locus_tag="ACG8KN_000082"
     CDS             complement(22118..23209)
                     /locus_tag="ACG8KN_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP9187181.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(23310..23744)
                     /locus_tag="ACG8KN_000083"
     CDS             complement(23310..23744)
                     /locus_tag="ACG8KN_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9187182.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24328..25785)
                     /locus_tag="ACG8KN_000084"
     CDS             complement(24328..25785)
                     /locus_tag="ACG8KN_000084"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9187183.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25782..27677)
                     /locus_tag="ACG8KN_000085"
     CDS             complement(25782..27677)
                     /locus_tag="ACG8KN_000085"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9187184.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27735..29510)
                     /locus_tag="ACG8KN_000086"
     CDS             complement(27735..29510)
                     /locus_tag="ACG8KN_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9187185.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29560..31056)
                     /locus_tag="ACG8KN_000087"
     CDS             complement(29560..31056)
                     /locus_tag="ACG8KN_000087"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9187186.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31250..32872)
                     /locus_tag="ACG8KN_000088"
     CDS             complement(31250..32872)
                     /locus_tag="ACG8KN_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9187187.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33200..34513)
                     /locus_tag="ACG8KN_000089"
     CDS             complement(33200..34513)
                     /locus_tag="ACG8KN_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9187188.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34794..34982)
                     /locus_tag="ACG8KN_000090"
     CDS             complement(34794..34982)
                     /locus_tag="ACG8KN_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187189.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKE"
     gene            complement(35002..35424)
                     /locus_tag="ACG8KN_000091"
     CDS             complement(35002..35424)
                     /locus_tag="ACG8KN_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9187190.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35569..36279)
                     /locus_tag="ACG8KN_000092"
     CDS             complement(35569..36279)
                     /locus_tag="ACG8KN_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFP9187191.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36297..36794)
                     /locus_tag="ACG8KN_000093"
     CDS             complement(36297..36794)
                     /locus_tag="ACG8KN_000093"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9187192.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPKHLADYLTQAYAPEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36831..37283)
                     /locus_tag="ACG8KN_000094"
     CDS             complement(36831..37283)
                     /locus_tag="ACG8KN_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9187193.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37418..38023)
                     /locus_tag="ACG8KN_000095"
     CDS             complement(37418..38023)
                     /locus_tag="ACG8KN_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9187194.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38578..39213)
                     /locus_tag="ACG8KN_000096"
     CDS             complement(38578..39213)
                     /locus_tag="ACG8KN_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9187195.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKKSMTISNKEMA"
     gene            complement(39215..40084)
                     /locus_tag="ACG8KN_000097"
     CDS             complement(39215..40084)
                     /locus_tag="ACG8KN_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9187196.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYISMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(40107..40514)
                     /locus_tag="ACG8KN_000098"
     CDS             complement(40107..40514)
                     /locus_tag="ACG8KN_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9187197.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELYENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRSD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40533..41705)
                     /locus_tag="ACG8KN_000099"
     CDS             complement(40533..41705)
                     /locus_tag="ACG8KN_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9187198.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAILWMATMTTVFGY
                     FAGLRGIVLILVGGFIGACFAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVA
                     KLFGEKGENKKSIEDIKLPTHFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGD
                     QNYIMFAFLQAIQFVVGVYVLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFP
                     YSPNAVILGFITTTIGTIIAMFTLPMFGLAMILSGMLTNFFAGGTAGIFGNAVGGRRG
                     AIIGGIAHGFFITLLPALLVTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(41732..42034)
                     /locus_tag="ACG8KN_000100"
     CDS             complement(41732..42034)
                     /locus_tag="ACG8KN_000100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9187199.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42059..44145)
                     /locus_tag="ACG8KN_000101"
                     /pseudo
     CDS             complement(42059..44145)
                     /locus_tag="ACG8KN_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
     gene            complement(44634..45399)
                     /locus_tag="ACG8KN_000102"
                     /pseudo
     CDS             complement(44634..45399)
                     /locus_tag="ACG8KN_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
     gene            complement(45510..46379)
                     /gene="mdcB"
                     /locus_tag="ACG8KN_000103"
                     /pseudo
     CDS             complement(45510..46379)
                     /gene="mdcB"
                     /locus_tag="ACG8KN_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
     gene            complement(46404..47488)
                     /locus_tag="ACG8KN_000104"
                     /pseudo
     CDS             complement(46404..47488)
                     /locus_tag="ACG8KN_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            complement(47512..48105)
                     /locus_tag="ACG8KN_000105"
     CDS             complement(47512..48105)
                     /locus_tag="ACG8KN_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFP9187200.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWATEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(48109..49785)
                     /gene="mdcD"
                     /locus_tag="ACG8KN_000106"
     CDS             complement(48109..49785)
                     /gene="mdcD"
                     /locus_tag="ACG8KN_000106"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFP9187201.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDFIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEETIVISIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKFRQAGYPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(49775..50074)
                     /locus_tag="ACG8KN_000107"
     CDS             complement(49775..50074)
                     /locus_tag="ACG8KN_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFP9187202.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(50176..51822)
                     /gene="mdcA"
                     /locus_tag="ACG8KN_000108"
     CDS             complement(50176..51822)
                     /gene="mdcA"
                     /locus_tag="ACG8KN_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFP9187203.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVVADKADKDGNL
                     YTGFNTEETPTIVEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADEPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALVVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLREAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(51829..52740)
                     /locus_tag="ACG8KN_000109"
     CDS             complement(51829..52740)
                     /locus_tag="ACG8KN_000109"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9187204.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLTFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEMVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            52860..53716
                     /locus_tag="ACG8KN_000110"
                     /pseudo
     CDS             52860..53716
                     /locus_tag="ACG8KN_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(53903..54265)
                     /locus_tag="ACG8KN_000111"
     CDS             complement(53903..54265)
                     /locus_tag="ACG8KN_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9187205.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(54454..54636)
                     /locus_tag="ACG8KN_000112"
     CDS             complement(54454..54636)
                     /locus_tag="ACG8KN_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187206.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            54611..56048
                     /locus_tag="ACG8KN_000113"
                     /pseudo
     CDS             54611..56048
                     /locus_tag="ACG8KN_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(56055..58607)
                     /locus_tag="ACG8KN_000114"
                     /pseudo
     CDS             complement(56055..58607)
                     /locus_tag="ACG8KN_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(58721..60052)
                     /locus_tag="ACG8KN_000115"
     CDS             complement(58721..60052)
                     /locus_tag="ACG8KN_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9187207.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYSLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELAAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQMIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCTHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(60018..61496)
                     /locus_tag="ACG8KN_000116"
     CDS             complement(60018..61496)
                     /locus_tag="ACG8KN_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9187208.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPKKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(61801..63636)
                     /gene="tdc"
                     /locus_tag="ACG8KN_000117"
     CDS             complement(61801..63636)
                     /gene="tdc"
                     /locus_tag="ACG8KN_000117"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9187209.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTTHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHMTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT        18235 a        13696 c        10804 g        21143 t
ORIGIN      
        1 ttgaacttaa ggttgattga tcacatctaa aagcgttgaa aataaaatgg cactagaagc
       61 agctcctggg tcgatatgtc cgattgcacg ctctcctaga taggaagctc gtcctttttt
      121 agctaccaag actttggtgt gttcttttgc ctggtcaatg atttcttttg ttaaattccc
      181 tgccttcaat gcctctgtga ctggcagcca aacatctaac atcgttttat cgtcagcttc
      241 tgcctttcct cggctttgaa tacccgctgt tccctgctcg atcacttcgc ctaatttttc
      301 ttgagaatca atagaatcga cctcttttgt cgctttcatc atgttcatga atgcagatcc
      361 gtataacgga ccagaagccc caccgacttt cgatatcatc gccatagaga atactttgaa
      421 tgtatcgctg attgtttcag gatgatcggc ttgaaaagct tcttcataag ctgaaacccc
      481 tcttcccatg ttatttccat ggtctccatc gccaatcggg gtatctagat cactgagata
      541 ttgtttgttc tgattgattg cctctgcaaa ttgcgtgagc cattcttgga tttcttgaac
      601 ggttagtttc atattatcac ccttttatct tgagattttt taccatgaaa tcgtctgtac
      661 attgctttct aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg ttagagaaag
      721 accttgcata tctagtgagg tcataaaatt accgactttt cggaaaacta cattgacgcc
      781 tctttcttcc aacagagcta aaacatcatt catgaacacg aactgttcca tcaaaggcgt
      841 cccgcccatt ccgttgacta gtacacctgc ttcttttggt aaattcgagt aatcatctaa
      901 aatcttagta acaagttcct ttgccagtac tttagaaggc tgcatctttt ctcgacgata
      961 gcctggctca ccgtggatac caacaccata ctcgatctca tcttctccta gttcgaatcc
     1021 tggtttccct acttctggta cagttgctgc tcgaagcgct agaccgatcg tcttagttgc
     1081 ctgtacgacc ttttctccca gttctttcaa ctcagctagg gaagcaccgt ttcttgctgc
     1141 atcgcccacg attttgtgta caaggacagt acctgcaact ccgcgttttc cagcagtata
     1201 cgtactattt tcaacggcga tatcatcatc tacgatcaca ctttctactt caatatcgtc
     1261 catagcagct aattcctttg ccatatcgaa gttcaatgca tcccctgtat aattcttaac
     1321 gatcagcaaa actccttttc cactatcggc ttccttaata gccgtctgaa tctgatctgg
     1381 agttggagaa gtaaacacat caccaagcac cgctgcactt aacatccctt cccctacaaa
     1441 cccggcatgg gatggttcat gtccgcttcc tccaccagaa accaaaccaa cttgttccgc
     1501 tttctgattc ttggctacaa cacgggaaga ttctacacga tgaacaagtt ctggatagct
     1561 ttttactaat ccttccagca tttcttcgac aatattgctt ggatcattca tgattttttt
     1621 caatttattc ccatcctttc tttccggcat tttgtgcttc tgttaagatt tgctgagtat
     1681 catctgttat accggcagtg atggctgctg caaaagctcc ttccactaaa ggcgcatcca
     1741 ccagatgata acgttgctgc tgctcttctt ccaacatgtc ttttgccagt tccgagttca
     1801 ataccgcgct accgatatcc gcaaaaacaa aataactagc tgcatcgctt gattcattga
     1861 ctgcttctac gattttcatc gggtctgatc cgatctcgcc atttattccg ccgagagaaa
     1921 aaatttgcac ctcttcggat ttttgcatct gctcaatcat ttctttaata ccatctgtta
     1981 ctttttgact atgagaaata agcaaaatac tttttttcac aagaatcttc ctttctttta
     2041 catagttgct aacgattggt ttcgctttca ttaatgaaca attcaagtat ataataaatt
     2101 tctaattttg ggaaaatatg tgtagtgctt cttgaagttt gttatacttt aattaaaaga
     2161 gaggagtgat cgaatgatta caacaacaac gaacagtgta gagggaaaag ccgtagtagc
     2221 ttataaaggg atcgtttttg gtgaggtcat tactgggatc catgcattta aagacttgga
     2281 ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg aactaatggg
     2341 tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca aaagcattag gagctgatgc
     2401 gatcattggc gtaaaaatgg actatgaagt attaggcagc gacaatggca tgctgatggt
     2461 cacatgtagt ggaacagctg tctccacgag ataagtaggg gacatctgtt ttaataacta
     2521 aagagagatg ggacatgtag tggcactcaa gaattagatt tttcagtcta acttcgaggt
     2581 actctacctt cccatcctct cttttttatt tctttttgcg gatgattccg taagctgctg
     2641 cgactaacac acccgcccct gctaaggaat aactgacaga agctgttttt tcatttgttt
     2701 ttggcaaagt ctgacttgtt gatttggatg gctctgtcgt ttgctgaact gtttctgtcg
     2761 tgcttccaga ggtcactgat ttttctcctg ccgaatgcaa aatatctttt attcgttgga
     2821 aactagctgc taagtcagct ttttttgctt caaaatcatt agcaactgtt tgtgcttcgc
     2881 tgatctttcc gtggattgct tgactggccg ctgtgatatc cgcttgagtt tgacttagct
     2941 ttccagaagc tgtttgagca acctgattta attgctgatt gaattctttt gcttgttgga
     3001 tcttcccgtc aagatctgtt tggacagctt ccgcttttgc tttcgcttcg gcaagctttt
     3061 tctcaagttg tgcacggtca tttcccactt tatttttaag atcagctaaa tcttgatcga
     3121 ttgctgcttg gtcttctttc aatttgtttt ttgcagcttc taatgcttct tcgagttctt
     3181 ttcttttatt ttccaagtct tgcaaatgat cgatttggtc agaagaggta gaactattta
     3241 ttgatgtatc ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt acattggaag
     3301 tcatttctgt ttccgaggca ccgttttcag caccagaaga gcttatggtt gttgaaggtt
     3361 ctgtttgagc aggttcagaa gtttccgcaa aagctgtctg tcccaataag aaaatactgg
     3421 aaacagcaat tggccagatt aacggcttca ttcttttttt catcactttt cccatcctta
     3481 ctacttttta actaatttct tattataagc aaaaatgttt ggaagaacca taaacacaac
     3541 aggctgtttg tctaaaaaac ggtgcatctt ttattttttt gtacgttccg ggcttttcca
     3601 tgtcccgctt ccataaccta aaatcgtttt gactgctgga atgaaagtcg tcacgatcgt
     3661 tatcgcgttc actatccaga acaaaagcat atatagtggt gcgaaaagaa ggtatttcaa
     3721 ttttcttcgt cgatcatcta caatcagcga agctagcaac tggaaaaatc ctgcgaccat
     3781 ttcaaagcag atgaacacaa aactgaatgt aaaagtaata taaatctgtt cgtagtttcc
     3841 ttgatataca taatgcccga ttaatccagc aaaaagaact acggaaatac aaaagaatat
     3901 tgaccagaca atactcaatg tttgatcaat gaagatgatc gttcttccaa tatgtttaaa
     3961 aggatggagc atcacttttt tgaaattcgt cagccagact tctgttccac cttttgccca
     4021 acgttttctg tgtcgatata gcatcttcaa cgtcacaggt acttccatga aaaagataat
     4081 ctcaggagca aaaacagata accagtcatt taactgatga tcccaagcaa tactgatgtc
     4141 ttctgtcgct cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt ttcgatacat
     4201 cgtatttgct ccactgtacg cataaagacc accgaaaaca gctgactgtg ttcgtttaat
     4261 gatgcccacg atacttgaga attcaaccgt ttgagatttt gcaatcaatt tggtacgatt
     4321 ttggacatcc atatttgcag tgaccgcagc aatatgtctt gcaccagggc gaatgaaata
     4381 attcacatac ctaatcagtg catctggctc cggtaccgtg tctgcatcat tgcttaaaat
     4441 caatttccct tttgcaaagg caagtccgat attaaacgcg tgggcttttc ctttattttt
     4501 ttcgatccga accacacgaa gattagcgta ttttttcatc agtctagcta atatttctgg
     4561 tgtttggtcc gttgaaccat cgtctgtcac taatacctca tagttgtgat aattcaattt
     4621 cgtcatcaaa tactcgattg tatattcgat cacaatttct tcattatggg ctggcaccat
     4681 gatcgtgatg aacggttcga cactcaaggg aacatctacc cattcttttt gcttatgttt
     4741 gtataaaaac acataacacc agacaccaat aaaccataca aatccgccta aaatcggata
     4801 agaaacgaga aaaaaataca taatagacaa acaagtatct aagaataaat tacctgtatg
     4861 ggtgattgag atcatttcag ttccacccca ttttctttat acaaatctgc aatgaatgtc
     4921 gtatctagat ttttctcttc ggagacagaa taaaaacgga ccgtttctct ctcttctttt
     4981 gttccaaaac gttccgtata cacttcttca agtaattctt gtcttttttt caatttttca
     5041 tcttcatatt caaaagattt atttactttt ttgtatagat ttcgattgtt ccacctagtc
     5101 aatacaaatg aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc gattaagaaa
     5161 tacagcattt tcttttctga taaaaaagcg tacaaggaaa aatccttggc tctttctggt
     5221 ctcgttaatg ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca tcctaaaaga
     5281 gccactaacg tttgccaaat ctttaaccag taattacctt ttgaaaaata cttatctttc
     5341 accaaaattg atgcaacttc ttctcttgtt acttttttca tatcctcacc tcacgttttt
     5401 ttgttacgac gtaggagttt accatacatt tcatttcttt tcacataaag tgttcctaaa
     5461 tttttcctaa gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata ttttgttctt
     5521 tttatcagtt gttcttttta ttttttatct cttaattatg ttgctctttc caatacgaca
     5581 atgcttttca ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct ttgctcatct
     5641 ccatcgtttt caaatggata taagattaca tgatcaaatt ccagtccttt tgctagactc
     5701 aaaggaatga tttggcagtt ttctaatcca tatgaatttt ttttaatcga ttcttgtagc
     5761 gtttgggttt cctcttctgt ttgggtgata actgccgctt tttctccttt tagagaaacc
     5821 aaaatctcag ctagtatttt caacaattct tcagaggatc gaaaagagcg gtattcgggt
     5881 tcttttcctt gcggtcggat cgatacgatg tcgatcttct cttgatccac agcataggtt
     5941 ttgaaaagct ctgtgattgc tccgctagaa cggtaactat ttctaagatc ataccgatgg
     6001 atcggcagat ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt ggaagaatta
     6061 aagattgcct gattctcgtc acctactaaa gtaaagtgag tttttggaaa taattcgatc
     6121 aatagaccta attgtgccag ggtataatct tgtacttcat caatcaatag atagcgaaat
     6181 gctggtacat ttaccttttc aacaagtaaa tgacgaatag ctaatataat gactgcttca
     6241 tcaacagtac gtgtgggttg ccaagcataa gctgagccat atcgtttttc ataaattctt
     6301 tcaaacaact gatgctcatc cacccatgcc atttcttcga tttgtctggt tatttttcga
     6361 tatttttttt gtaaaagttt ccttgcataa gctacaatac tttgttccga atcatcagaa
     6421 atcaatttac cgaataattc caactgccta tcttctgtca acgagagtac ctgattctgc
     6481 acagcaggac tttttgcttg tttcaaaaga tgattcttcc attcgctcat cagtgccttt
     6541 ttcgttgctt gaagcctatc gatcaattta ggatgagcag gaactttctc atatattttt
     6601 tcgatcgttt ttttagaaag gactgttttt ccatttttag taagtccacg gataaaattc
     6661 ggatgatcta aaaacaaagg atctgattgt tttagattat cgataaactt tttgctacgc
     6721 aaacgttctg tctgctccga tacattttct cccgtgatac gtttgaaata agcctcttca
     6781 ccttctattt cctctgctga aagctgtgaa accagctgta tgatggttag attcagtgga
     6841 tttctttcac ctaatgatgg taggacattc gaaatatagt cgataaaccg atgattaggt
     6901 gatagaatca gcaagtcatc ggatgtcatc gtatctcgat ataaatagag cagatacgca
     6961 atacgctgca tgatagcaga ggttttacca cttcctgcta cgccatttac caagatattt
     7021 tgacttttca catcccgaat gattgtgttt tgttccgatt ggatcgtagc agtgatagcc
     7081 ttcatttcat tggtctcatc ttggcctaag acttctaata atacgtcatc ttgaatagct
     7141 acagacgtat caaaaaactt gaccaaacga tctctttcca aaagaaattg tcgtctttct
     7201 ttaataaccg tatcgatttt attttgacga accgtataag aagatggacc aatcatgtta
     7261 ttgtaaaaaa gttcggcaat tggtgaacgc caatcataaa caagattttc atctgcatca
     7321 tctgtgaaat ttgctgtacc gatataaaag gattcttttg gctcattatc cagaaagtct
     7381 acaacgactt ttccaaagta gggacttttc aataaacgtt cgatctttgc tagctgattt
     7441 tctgcgtttt tctgtttgat attcatctga tcgatttctc tatttttcat ttcaatcatc
     7501 gaaaaagtat ccaggttatc taagtaactg tcaaaattca atcgaagctc gccagccatt
     7561 tctttcaacg actggtttcc ttctattttt gccgtatcca tcgattcccg cagttcgttt
     7621 tttttatcaa ttagcttttg atacacgttc ttcagatatg cttcttctaa ctgtttttgt
     7681 gtgttgacca tacacattct cctctttttt taaagtggtg gaaccgatta gtttacctct
     7741 tttaagagtc gaatgcaagc tttttaacga gtatcatttg ttaggatata tcagttcttc
     7801 aggtcggaca gtttgatata aagaaaatgc accatcaagg ttttttacgt ggtagccaga
     7861 ctgcttcaag attctttccg ctagatagct acgtaatccg ctatgacagc tgacgatata
     7921 ttcttgttgg ctgtctaatt catttaatcg gtcccgaagt tcattcaaag ggatatgttt
     7981 ggcttccttg aatttgccct tttccaattc ttctgctgtt cgcacatcta ataaaatttt
     8041 tccttttgct agttctgtcg gcaattcatg ccattgtacg gtttcactaa gcccttctgc
     8101 aatgttcatt gctgcatatc ctagcatgtt tactggatct ttggctgagc caaatggcgg
     8161 tgcatacgtc aattctaatt ccggtaaatc gaagatcgtc aaatgccctt tgattgctgt
     8221 tgctaaaata tcgatccgtt tatccacacc ttttgcccca acaccttgtg caccataaat
     8281 ctcgccagtc tctggatgga agatcaactt cagtaaaata tctgttgctt ctggataata
     8341 gcttgcatga tcttttcctg atacatgaat gacttcaaaa gacagttcgt tttctttggc
     8401 tgttctttca cttaaaccgg tggatgcggc agtcattcca aacgttcgta taattgctgt
     8461 tccaataccg ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa cttgtctacc
     8521 ttgacgattg gctggtgaag caagagaaat aagtgcatct tttccagtga tttgctgttt
     8581 tacaacgata gcatccccca ccgcataaat atcaggaata ttcgtttgat aacgttcgtc
     8641 tactaggata cccccgcgta tgcctaattc gattccagcg gtcactgcta gctggttatc
     8701 aggacgaaca ccaatggata aaataactaa atcagcaggg atggtacgcc catcatccag
     8761 tcgcaatcgt ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa tcacttctac
     8821 tccttgatga gacaattctt tttctataaa agctgccatt tcttcatcca gtgttggaag
     8881 aatatgtggt gcttgttcga tcaccatgac ttctaaacct cttcttttta ggttttctgc
     8941 catttccaat ccgatgaatc ctgcaccaac gatcacggca cgctttggct gtttttctaa
     9001 tgcatgcatc actttatcga tatctggtac atttctaata gaaaaaacat tgtcggcttc
     9061 tgttatccct ggaatcgatg gaacaactgg ttttgcacca ggagataaaa ccaatgcatc
     9121 gtactgttca atgtgttttt gaccctcatg tacgacttct accgttttgt tttcgggaaa
     9181 gatggctgtc acttcatgat ttggacgcac atctaaacaa aaacgagcgg ataatgattc
     9241 tggcgtttgg acaagaagat tttcccgttc actgatttct cccgaaagat aataaggcaa
     9301 gccgcaattt gcaaaagaaa catacggtcc tttttcaaaa acaacgattt ctgcatcttc
     9361 cattaatcgg cgaagccgtg tagcagcaga cattccacct gctacacctc cgacaataac
     9421 gattttcatc ttatttccct cctactgttc gatcaaacca ttgattcatg ccaccacgta
     9481 cattcactac atcataacca gatttcttta attctttagc tgcttgttta ctcctcatcc
     9541 ctgattgaca aatcacatag acggtctttt ctttgtctcc atcatatctg tttatacttt
     9601 gtaatggaac atttttagct ccttttatat gcccgccccg gtattctgca ggtgttctaa
     9661 catcaagtaa cgctggattt tccaaaagct tttcctttaa ttctgggact gttatcgatt
     9721 ttattgattg aaatagcaaa ttcattttct gcacctcatt tacattttta taaatacctc
     9781 atggggtata ttaaacagga gctttattaa tatgtcaacc agaatgactc ttcatttttt
     9841 tcactacttt ttatatttgc ataaaatacc gataaggggt attatctaat tataaaaata
     9901 gaggagaatg tgttatggag attgataata aaaatatcct gaaccgtttg aaacgtacag
     9961 agggacaaat tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt atggatgtca
    10021 tcactcaatt aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg atcgttgcag
    10081 ataatctgaa aaattgcttt gagaaacctg atagtaatcc agaagaacaa gcggctaaac
    10141 tagaacaagc gatcaagatg attattaaaa agtaaaggaa tgatttcaat gaaagtcgtt
    10201 ttccatattg atgaattaga aaaatggtca gaaacaggga aaaacgtaaa aaacctgata
    10261 aaagcttctc cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat taccggttat
    10321 ctagactctg ccaatgcaga attcttagat ttgcaaggag tcgtttttca cgcttgcgcc
    10381 aatgctatgc gtgctaatca tatttcagaa agttctttac ctgaacaagt aatcgttgta
    10441 ccagcaggtg tccttgattt agttgaactg caatcccaag gttatgctta tatcaagcct
    10501 tagtcagtag aggttgcgac agaagtgatc agcttcaaga aatttcggct tatttttcga
    10561 agaagctact tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc tgcatcaagt
    10621 aataagaaca gccaaacaaa aatagcttct catatttttc gtttggttgg gcttattacc
    10681 gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaatcagc ggtttgacct
    10741 aagtattaga gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt ttgagctaat
    10801 accgaaggtc agctgattga acaccgttta aggaagagat tgtgaaaaag tcgcttagtt
    10861 tcgagtatta agaaaaattt tgtgaaaatg gatttctaca tttttgcata aatttggctt
    10921 aataccgaga aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa tcagcggttt
    10981 gacctgagta ttagagaaac aaacggaaaa atagctgctc atattttgcc gttttttgag
    11041 ctaataccga aggtcagctg attgaacacc gtttaccaag aggttgtgag cctaccattt
    11101 aactccgaat cacaagaaat aatttcaaaa acaacttctc ctgtttttgg ttcggttggc
    11161 ttattatcga gaagtagcta ctgggagtct atttatcaaa aaaaggaatg cgacataggt
    11221 cacacccctt ttttattttc tttgaaacat ctttcggtac tcgacagcat acaagacaaa
    11281 agattgtaaa agaccgtaag tgatgacggc aaaaatgact ctcacccaca gcgttggtaa
    11341 ttctgggaaa agcagcaaaa ggatcatgcc aacatagatg acagcagtcg ctactttgcc
    11401 ataccacttc gctccactta atttgatatt tttagatagc ccgattaatc cagctacaaa
    11461 cataaagcct tcttttatca agaatagacc aaacaacagc caaagccacg gtcgatacca
    11521 tgccatagac aaaatcagaa aaagttgtgt caatttatct gcaaacggat caagcacctt
    11581 ccctaagtct gttacctgat tatatctccg agcaatgacc ccgtctagat aatcagacaa
    11641 acctgagaca acaatgaccg ctcctgctat ataaaatgac tgtctcagca caaaataaag
    11701 ataaatcggc agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg tttgtatttc
    11761 tttacgccag tctgtgctta atttcatttt atcgccctac ctcctcagca ctcttttctt
    11821 tttattttag cttacacggg gaataaaacc aatgatttca ctgtttttat ttttctttac
    11881 agacactgat attatgagct aatttgagat aatcacttgc tgttttcatc gtttactctt
    11941 ctttcttcct gtcgtttgcg catttctttt actagtactt tatgagcttt ttttgatcct
    12001 cgctctaggt cacgctgccg tttacgtgtt ttataatcga taaacagact gtctaaatcg
    12061 taacctgtcg ggtacagatc ttctgctgaa cgcaccagac gaacacgctg acgaggcacg
    12121 tggattattt ttttgtctaa atatacatca atcgtatctt cacctatatc ttcataaact
    12181 aacgcttctt tttggtattc attgacaaat atccgatcac cttttgcaaa gagctgacgt
    12241 tcttttttat tctccttcct atttgtaact gctgtcttag gaagaaattc ttttttctgt
    12301 gtccgatagg ctttttccgc caagtaacgc tctgcttgat ggatcaattt catcgacata
    12361 tccattttat gagcaatcca caaagcttgg ctttctcctg tttcttccaa cccgcaactg
    12421 atatttagga cgaagtgctt cccgatcgaa agccatagca gccggcacaa aatcttcatg
    12481 atctccggcg aatttcttga tttctccata atgggtcgtg gcaatgatca atgccccttg
    12541 ctcatacata gattccatga ttgcgatcgc tagtgcagcc ccttcatttg gctctgttcc
    12601 actgccaatc tcatctaaaa gtaccaacgt attccttttt atctttttca aaatagcagc
    12661 gatgttttgc atatgaccag aaaatgtact caacgcattt tctaaatttt ggtggtcacc
    12721 gacatcgacg aaaatctcat caaagacagc aagctctgtc ccttcttttg ccggaacttg
    12781 cataccgaac atcgccatca aagtcaataa accgactgtc ttcaacacga ctgttttgcc
    12841 tccggcattt gctccggtga taatcaagcc tcgatagtct ttaccgattt caacatccaa
    12901 tggtaccgca tgctcaggta aaaatggatg tctgccttgc ttgataatga tatgttccga
    12961 tttattgatt ctaggtgtgc ttccattgat ttctcggcta aattttgccc gagcaaagat
    13021 gatatctaac accgtgatcg tttcgatcaa taaatcgatc acttcttcgt tttctgcgat
    13081 agcaccagtc aatgccgcca ggacttgata ttcttcactg atttcttcag cttttaacaa
    13141 ctgatagtgt tcattcaatt tagaaatcac agttggttca ataaaaaccg tcgttctttt
    13201 atttgattca tcgataatcg ttccatctat tttattttta tagctggctt tgataggaat
    13261 cgtatagtat tcgccttttt gaacaatcat tgcttcttgg atcatttctt tgtttttcgg
    13321 atgacgcaag aatttcagta attttgactg gatttctttt tcgattagtt gtaattgttt
    13381 gcgtacttta cgcaaattac gtgacgcgtc gtcactgact ttattatttt ttattttctg
    13441 gtcaatcaac tcttctacat ttatcaaatc aggaagatgt ttgctataag caaacaacaa
    13501 gggtgtctgg tattgattct tatcaaaaaa ctttgtgatc atccgactgc ttctaagaaa
    13561 atccgcatat tcgatcaaat cagttggctg cagaatcaac ccttttttga cttgttctgt
    13621 cagtgcatcg atcctcggca accccataaa tggaacatgc tgactgcttt ctaagatcaa
    13681 gcgtgcttct cttgtttctt cttgacgatc caagacagtt tgtaggtttg tcgagaccgt
    13741 catatcgctg attcgttttt tgctatagtt tccaattgct cttgcttgga tttccttttt
    13801 gatccgatca aattgtaatt gactaatcat ttcgttgtcc atattctctc cttttcttga
    13861 agagatacct gtcccaaaat aaataagggc ctagagataa ataaaaggat agaacacagc
    13921 ttaaaaaagt catgtcctat ccttattaga atacaaagat attgttttcc tgacaggtac
    13981 cgtttcataa gcacaccaaa taaactttgt ttattggcta ttcgacttac tgattagata
    14041 ccagctacac tcacaaaaca taccccattc ttctaggtct tatttatttt ccacttaact
    14101 atagcaagac acattttggt tgtcaaactt tagtatgttt tatagattta caccattaat
    14161 gaatagacac ctaccgcgat aattgctccg atcattggac ctgcgattgg tacccatgaa
    14221 tatgcccaat ctgaagttcc tttattagct attggcaata ccgcatgtgc taatcgagga
    14281 ccaaagtccc gagcagggtt gattgcatat cctgttggcc ctcctaatga aagaccgatg
    14341 gctaagatca atccacctac agcgaatacg ttcgttccag gtgcaaattc gttttgtcca
    14401 aaagctagca acccaagaac taatacaaat gtaccaatta attctgtgat cacatttgct
    14461 ggataattcc gtatagcagg acctgttgca aacgttccta aaatcgcgct ttcatctttc
    14521 gttgcattcc aatgtggcaa ataagaaagc caaacaagaa tggcccctgc aaaagctcct
    14581 aaaacttgcg caatgatgaa aggaacgaca agattccaac taattgcccc tgtcatcgcc
    14641 atagctagag agactgcagg attcaaatga gctgggccca tgaatccaga aatatagaca
    14701 gccattgtta cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga agcctgtgct
    14761 ttacttttgt ttaaattcac agccgcacac acaccatctc caagcaaaac taggatcagt
    14821 gtcccaataa actcccccat gatttgagtc atatcactgt tcatttattt ctcccccttt
    14881 agttcccgca gatctgattc tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc
    14941 tcctcttctg tccattcaaa atatccagca attgcatcga tcacaggttg cttgatctca
    15001 tcaagctgat ctcgttcaaa caacagatga tttgtacgac gaatgagata atcgcctggg
    15061 gccaaaacca tttcttcttc taaaccataa cgtaaccgag ctgactcagc gagactcaaa
    15121 ccaggataag gtgccatttc ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa
    15181 aaatcagcga tataagtagc atcttcttcc tctaaaccgg ctgctactcc ttcttttgct
    15241 aattctgtca ctgtttcttc taatttagtt ggatcgaagt ttccacctga aatctgatat
    15301 tttttagagt cgatttcttt cgtctctatt ccgtattctt cttttaataa ttggcgtatc
    15361 aatcgcaaag ctccttctgc catcttacgg taatcggtga ttttcccacc agataaagtc
    15421 agcaaaccat ctggttctct ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt
    15481 tcatcacggc tgttttccaa atggttcaac acgtcttcta cttcagcacg agaaactttg
    15541 ttttccttat attcacttac tgtatcgaca actttattga agctcttgtc tgaaatcgat
    15601 ccattatctc caccattgta atcagaacca gaattaccaa tcaacagtgg acgaagtcct
    15661 gcccagcttg cttcgatatc attcaatgtg atatttgctt ctggatagcg atgattgatc
    15721 acatccaata gataatctac atcttcttgt gtgactttcg gatccgtaaa atctccttgg
    15781 taatccgtat ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc
    15841 cgcccatctt gctttcctgt atcgaagtat gtgggttgcg gtacaggcag ttttttcgca
    15901 tctacaacta aatggatccc tttagttgga cgcattttag gagagactgg acgcgtaaaa
    15961 ttcaagtttc gtactttatc tacccaagga ccactcgtat tgatcactaa ttttgctttg
    16021 atttcgatca cttcatctgt cagcaggtca cgagctttga cgccaacgat ttgatcgcca
    16081 tcatataaaa agccaaccgc tttcatttta cttactagat aagccccatc ttcggcagcc
    16141 tttttgatat tatcaatcac taaacgggca tcattgttgc ggaaatctag atagacaccc
    16201 gcgcctttta gcccttcttt tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc
    16261 agcgtataat tctcatattt ggttcctgtc acatttgcca gtttgtcata aaggtccatt
    16321 gctactttga ccgagaacat attgaaggtt gttgctcctt cgccctcata gattggcaaa
    16381 agcattggat ctggttttgg gatgtgtggg gcaatacttt gtacgactgc acgttcacca
    16441 actgtgtcag ctactacttc cacatcaaat gtcttcagat aacgaatacc gccatgaaca
    16501 agtttggtcg agcgagagga agtcccttct gcaaaatcct gcatttcgat caatcctgtt
    16561 ttgattcctg atgctgctga ctggactgcc acaccggcac cagtgattcc gccaccgatg
    16621 atcaacagat ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt
    16681 gaaaacatat ggatttcctc cttattcaaa tgcttgggta gcttttactg cttttttcca
    16741 accgttgtaa agtttttctc ggcgctcttc ctccatttga acttcaaata gcttgccagc
    16801 ttcataaaat tcgcggatct cgtctgtatc tttccaataa ccgacagcta atcctgctaa
    16861 aaatgctgcc cctagcgccg ttgtttccaa gttttctgct cgttggatcg gtacattcaa
    16921 gatatctgtt tggaattgca tcagatattc attgtttgct gcaccgccat ctactttcaa
    16981 tacaggaatc ttgattccag tgtcatcttg catcgtatcg atgatatctc tcacttgata
    17041 agcaatcgac tgtaaagtag ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc
    17101 aaacatagcc cctcgtgctg cctgatccca ataaggagca cctagcccaa cgaatgcagg
    17161 aacaacataa acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc
    17221 tttttgcaac atttgaagtc catctctcag ccattgaatc gaagaaccag ccacaaaaat
    17281 acttccttct aaagcatagt aaacctttcc atttatgccg taaccaattg tcgtcaataa
    17341 attatttttg gataattgcg gtttttcacc ggtattcatg acaataaatg aacctgtacc
    17401 ataagtattc ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg cggcttgctg
    17461 gtctcctgcc atccctgaaa ttggtgcttc gcttccataa aaatgataac cttgcgtata
    17521 accataaact tctgagttag aagtgacttt tggtaacatc actcgaggaa tatttagtaa
    17581 atctaaaatc tcctgatccc aatctagctg atggatattg aacaacatcg tccgactggc
    17641 attcgagtaa tcagtaacat gtgcttgtcc atcggtcagt ttccatacta accaagaatc
    17701 gatcgttcca aacatcaatt cgcctttttc agctcgttct tgcgcgcctt ctacatgatc
    17761 aagaatccag cgtatcttcg ttgctgagaa ataggcatcg atcactaacc ctgttttttc
    17821 gtggaaaaat tcttgatagc cttcattttt taagttatct gcaattccag ctgactgtct
    17881 ggattgccaa acaactgcat gatagattgg acgtcctgta tctttttccc atattactgt
    17941 tgtttcacgc tggttcgtta tgccaatccc agcaatctga tttggtttga tccctgattc
    18001 gatgaacgct ccggcaataa ctgactgtac agaattccag atttcatttg catcatgttc
    18061 cacccaactt tcttgcggga aatattgcgt gaactctttt tgagaactgc cgatgtgtct
    18121 tcctttcttg tcaaagataa ttgctcgtga gctagttgta ccttggtcaa tcgccatgat
    18181 atacgttgat tctgtcattg tgattccctc cattatatgc aaacgctttc tttaactact
    18241 cttatcatac tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt
    18301 ttgacagttt tttattatga agctattttt taatcaattc ttttcttttt tcagcgtctc
    18361 tattcttctt ttcaatcgaa taatgtcgta aggtgacgca aattctgaca gataatattc
    18421 acactgtttt ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt taccttcttc
    18481 ataacttcca atctcaatac cgccgattcc cctcagttct ctgatcagat attgttgaaa
    18541 agcaatacga taaatcggca gactttctaa atcgattcca atcactactg gttttgcaat
    18601 ataaaaatcg atcatcaaaa gaacattggc atactggatc atcaggtaac taagctcact
    18661 atcttcagct cttttcctcg gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa
    18721 ctgtgttaag gaatgcccga gcattttctg ctgctgttcc aaaagccgat cacgctcata
    18781 gacttcgatc tttccaacaa agaaaaacca ttcattgttg atctgggaaa tttggtagcc
    18841 tactgccttt tcttctttga ttttcaaacg acgaggacga taatgccata acatcgtttc
    18901 ccgaatgtaa gtatctaaaa cagcggttgg taactttttg ctccgtgtca aatcatactg
    18961 ataaaatatt tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt
    19021 ttcgtataca ttcagttctg ctgctgttcg actaagatgc atcacgatga ttggatcgat
    19081 tgcttgataa agccggtccg actgatagag taatttcttt tctttcaaag ttgcgtacgt
    19141 acttttttca ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc
    19201 tgctgatgcc gtaaacgttg tttctaatac acggtccaat cctagaatga acggtctttt
    19261 ttctggtgta ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt ggaatagttc
    19321 ataataaaaa tatcgtatct gtagctcttc tccgtataat tgaccgtttt tgatttgtaa
    19381 tccaaattcc tctaagagct ggttcaattc tttgatcttt cgaaaaacag aagactcact
    19441 gatattaaac gctgtcgcta atcgaacgat ggatacttgc tgatgttcta ataaatagac
    19501 gagcatctta aacttcaagc tttcctgcaa taaaaacaat agaatttttt ccaaacttac
    19561 atcttcgtcc aacacaagca gcgcctgata ctcatttcgc tgtacttcta cttttttttc
    19621 catcattcgg ccaatcaatt caatatcttc caaatattga tcaaacgagg cttttgacag
    19681 ttggacatgc tctctcaaac cattgataga ggctgttccc ccttgcagaa caaggtttcg
    19741 caaaatcagg atctgtaact gctcttttct ttctaataat gcttcaatct tcataggcag
    19801 tattcgccac ctttgaccac accatatctg gcacacggta ttctggctgg aaaaatagct
    19861 catgttcttc taactgttca aacgtccatc cttcttctac tgcaattgtc agttggtcaa
    19921 gaaggtggaa catctctttt ttgctttgga gctgtgctcc aagaatcttt ttttcagaat
    19981 caaatagaaa tttgagttta actttgcttt gatttattgc tgtaagcttg tagtctacgt
    20041 atcctgtttc aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc
    20101 ctaagaaata gccaaaccac tgattgccga ctggacgata ggttccctga tcctttgtct
    20161 gtgagccact aattgtcttg ctgacatttc gtgctgtacg gacagcgtta tttactagag
    20221 aaacatacat tttttcattt gttggacgaa aagttacttg gatcgcatcg ccaatcgcat
    20281 aaacgtcttt ttgagacgtt tgcaagtagt catctaccca gatcgttcca tcatcattca
    20341 atgttaattg ttcttcccac atctgattgt cgggacgaga atgattagcc agcaaaacaa
    20401 gatcatagtt ctcctttctt ttttccgtct ctacaatagc ggtttcattt tcggtcaaag
    20461 acacaacttg ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc tttactaacg
    20521 gttctgtcat ttcaggatct agataacgaa aaagaattga ctttcggctt tcgtaaagat
    20581 ggatttgttt ttttcttgat gccaaccctt cagctatttc gattcctgct tgtcctgcac
    20641 caataattgc aattttcttt gcattcatga tcttttgctg aagagccgtt acgttttgta
    20701 tctttttaac tgggtgaata agcgttgatt ctgcagaaat atttcgaaac ttttgatttg
    20761 aaccagtcgc tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct aaaacgactt
    20821 ttttttcttt caatcctatt actgtctttt ctgtgtaaac atggattcga tagcggtcag
    20881 tcagttcttc tttcgtgatc caagaaagtt gtttttcact ggtaaccttt ccttttaata
    20941 tcaaattgaa cccgctggga atccatccta acttcgattg cttttcaatc aaatatattt
    21001 cacttgttgg catcaattgg cgaagataaa tagccgcatg aatccctgca aacgacccgc
    21061 cgacaattac aatgttcatc tgcattcctc tttcttataa ggtgttctac tgctaacttc
    21121 tcaatcagaa aacacagtat tcataactcg ctttcaacct tatgatacat agcgatgcag
    21181 cagaatcaac cctctccatc ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg
    21241 gacctaattg aagagtatca tttctgaaga gatgtttcaa aaaatgtact cttataaaag
    21301 aaaaaaactc gccttttacg tacctctgtc caaataggaa acagaaaaga acgaaaagac
    21361 gagttttagc gtgtgtaatg taatagttag aatagacaca tcatttattt ttaattcttt
    21421 aatgcttcaa caactgcttc gcctaattga tgagcagatt gaggattttg ccctgtaaga
    21481 agccgcccat caatttgaac cacttctgaa aaagctgctc cagactgata gtttgctcct
    21541 ttttctttca aagcatcttc tgctaagaat ggtacttcat tcgttgtacc atttgcttcc
    21601 tcttcttcat tactgaaccc agtcactgta cgtccagcta aaatagattt tccagactca
    21661 tctgtaactg gcaacaatcc tacaacaccg tgacaaacag ctgagaccag cccttcattt
    21721 cgatagattt gtgaagcgac tttcccaaga tctattgctt ctggaaaatc ccacattgtt
    21781 ccgtggccac cagcataata aaccgctgca taatcatttg catccacatc atcaggacgt
    21841 aaggtatttc ccaaggcccg atttctgaaa tcttcatctt cataataggc ccaatcgatt
    21901 tcatccatat tcaaaacact ttgcggttct aaaggtacat atccgccttt aggactgaca
    21961 aaatctgcat cgatcccatt ttttactaat acatcataaa aatgagtcag ttcacttaac
    22021 cacaaacctg taggacgctc gatcttttcg tacttttcta catttgttaa tacgatcaat
    22081 actttcattt tcccatcctc tttctataaa aattgcgtta ttgataacgt gctttcaaga
    22141 aattatataa tgggcttggc aaataaagct ctttatatgc ttcttcaaaa ggtttcgtta
    22201 caaattcatc ctcacggttt tccaataatt tctctgccca gtcaggatcg acgatcattt
    22261 gctgtccaat tgctgcaagt tctgcatatt tcaatacctg ctctacatct tcgcgattgc
    22321 ggatcccacc gacaccaact aaagggatct ttccttgaat cgtgttatgg accacttcaa
    22381 gaatcgattt atcgttgtat ttatcagaac gagctattcg atcatacata tttaatgaaa
    22441 catgcagata atctagtttt gactcacgta atcgttcaag aagccagatc gtgtcttcaa
    22501 aacggatacc tggtgtttca tattcttctg gtgagaaacg ataccctacg atgaatggtt
    22561 ttttagcata ttgttcaacc gctgagaaaa ccgttgttaa taattcttca ataaaacgat
    22621 aacgattttc caagctaccg ccccattgat cctctcgtct attcgaatgc ggagagaaaa
    22681 attgttggat caaatacgta tttgctccat gcaactcgat tccatcaaat cctgattcaa
    22741 tcgctctacg tgtggctttg ccaaatgctt ggattgtttc aacgatttct tcttcactca
    22801 ttgctcgtgg tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca gatacaggct
    22861 gttctccaga taaagttgct cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg
    22921 cttttgcgcc ttctccttgg attgctttcg ctagctcact taatcgagga atcatttcat
    22981 catgagcgat gctgagttca cctggccagc cttttccgat atcttggaca tttgcagcac
    23041 cagtaataac agcaccaaga cctttagaac gttgtttata ataagtgatc tcatcttcgg
    23101 tgatcactcc attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc aatcggttct
    23161 tcaattgaac tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt gtcattcctc
    23221 ccataattcg ctctgattaa cctatcgaga atcatctatc attaaaccac tcgattagtt
    23281 gtacatgcaa ctaatttgct taaaaaattt tatttttttt gtaacttatc tgttgcatca
    23341 ttcattgtct gggctaattc tttacttgct tcatttccca aaactgcttt gtatgactga
    23401 tgaagttctt cccagctacc atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga
    23461 taaactaatg acaagcgacc ttcgctaact gttttgacgt atcctttata ttctaatttt
    23521 tcaacaaaac gagcaatcgt tgatggggcg gaatagagca tttgtgcaat atctttttgt
    23581 ggtattccat ctttttcttt taatatcaat aaaataaaac cataagttgg tgccagacct
    23641 gtccttctaa acgcttcatc agctaattta ttcaacatgc gatctagctt tttcacgcta
    23701 aagaaaagac attcatctaa cgctttttca attggctttt ccatttcatc ccctccattg
    23761 gcgtacatat catagtatga tttttatttt ttgtcaaaaa acacggaata atgtccgcct
    23821 cctctgtcta gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg cttgttttcg
    23881 acaaaactcc ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag
    23941 tatgaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac
    24001 cgaaaaactg gcttttgaac accgtttata tcagaggttg tgaaaaagcc gtcctgcatc
    24061 aagtaataag aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat
    24121 gccgatggtc agctgattgt acaccgttta ttgagaggtc gtgagcctgc cgttgaactc
    24181 cgagtcacaa ggagcaattt caaaaaacag cttctcatgt tttatgacta tggtgacttg
    24241 tgatcgagga gttggcagga gaatactgtt tattcgtaaa gaaggggctg tgacaagact
    24301 tttgtcacag tcccttattt ccttttatta taggatttcg ccatttgatt ggatgacttg
    24361 tctgtaccaa tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc
    24421 ttgatcaaca tagatatatc cataacgttt tgacatttgt gaagtagaag cgctgacgat
    24481 atcaatgcag ccccacgacg tataaccaaa taaatctact ccatctttga tagcttcctt
    24541 catctgctca atatgctgct tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc
    24601 ttcttctact ttgtctatag ctccgagtcc gttttcagct atgaacagtg gtttttgata
    24661 cctatcatat aattgattca aagcaattct caaaccctta ggatcaatct gccagcccca
    24721 ttcacttgat ggaagatacg gatttttaat tcctccagtt attaagtttc cttggacccc
    24781 ttcccaattt tctggatgag cactgactgc actagacatg tagtagctaa aagaaacaaa
    24841 atcaacaggg taggctgcta agatttcttc atcacctact tctattttta agttgacacc
    24901 tttttcttca aacattcgtt gcgtatacgc tggataatat cctcttgctt gaacatcact
    24961 aaagaacaaa gaagttcttt gctgctcaat tgttttttga atatcatccg gatcacaaga
    25021 atacggaaca agaagctgat agctgatcat acaaccgact tgtgaacctt tgataatttc
    25081 atgacaagct tttgtagcta aagcacttgc tataaactga tggtgtgcac cttggtattt
    25141 cgcttgttcc aaatggggat ggttttcttc aattactcct acgctaacaa aagggtgatg
    25201 ttttacacag ttgatctcat tgaaagtcat ccaatatttt actttatttt tatagcgttt
    25261 gaatactgtt tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag gccatccacc
    25321 atattctgta accaaatata acggcatttc ataatgggaa agagaaacga ctggctcaat
    25381 cccatatttt agacattcgt caaacacttg atcataaaaa aataatcccg cttcattggg
    25441 tgtttcttca tcaccatgag gaaaaattct tgtccatgcg atagacatcc gatagcattt
    25501 aaaacccatt tcagcaaata aacggatgtc ttctttaaag tgatgataga aatcatttcc
    25561 gtgtctttta ggataataat acttgctgtt gggatcctgc gccgctttta cttgctgatg
    25621 cgtcatcata tgccattgat ctgtccattt ttctttaggt aaatcgctgt cgaatgtata
    25681 cacatcagac acagataatc ctttttcatc tacatcccat gcaccttcac attgattggc
    25741 cgctgtcgct ccgccccata aaaatccttc tggaaatttt ttcatatcga tccccctaaa
    25801 cttgctagtt ttcttgtttg attatttttc ttatgactag taatgatatt tttgaatcta
    25861 ctactatttg taacaataac cattgtggtc ggatcatatc cctctttgat taattcttgc
    25921 aaatctactt ccatcaataa atcgccttta cgtacttttt ttccatcaga tactgcactt
    25981 tgaaagtatt tgccatttaa attcactgta tcaataccga tatggatcag tatctctgct
    26041 ccttcttcac tgactacccc aattgcatgt ttcgttggaa aaactgttga aacttccccg
    26101 tttacagaac tgtagatttt tccgtccgtt ggaatgatcg caaacccttc tcctaaagat
    26161 ccactagcaa atgttggatc gtttacttca gataaatcaa ttatttcacc tgctgcagga
    26221 gtacttaaca ctatatcctt tgaggattct tcttcgattt catccttgta gccgaaaaga
    26281 aaagttagca tacaggaact gatcatcgct aagaaataac tagcaactag ccaaacaaat
    26341 ccttcaccaa tataaatcgg gaaagacaaa atacttcttg tagcaactgc caaagcaccg
    26401 gctcctgcgg caccagctac tccaccaaat atagctccac atatacaagc aatgtaaaat
    26461 ggtcgtttat atttaagagt cacaccataa acagcaggtt cagtgattcc aaataatgct
    26521 gtgataaatg cagataaaga taaacttttt actttcttat ttttcgtttt caaaaatacc
    26581 ccaagtactg ctcctgcttg cgcagctacg gcagtcatac atgctggacc tagcgtatca
    26641 cgaccgtaga aagaaagatt gttggtcatt actggtacaa gtccccaatg taacccaaaa
    26701 atcactaaca cttgccaaaa tccgccgata acagcaccag ataaaacagg gctaaagtta
    26761 taaacagcta aatatccagc tgcaatcagc tgacttgcat aatctgcaat tggacctaca
    26821 actaataaaa taattgggac catcacgatg atacaaatga atggtgtcaa taaatttcga
    26881 atactttcgt gcaagctttt gcgaatctta ggctcaatat aagaaagaac ccatacagct
    26941 aaaattgctg gaataaccgt acttgtatac cttgctagaa tcacgggcat ccctagaaat
    27001 cgcaaggtta tgctatcact atatgcactg acaatcgtag gataaacgag agctgctgca
    27061 ataaccatag cgatgaaacg atctgtattg aacttttttg cagcagtata tgccaaaaat
    27121 ataggcaaga aatagaaaaa tgcatcagct gctgcattca aaatgatata agttcctgat
    27181 tcagtagtta ataaattcaa actcgtacat aaggcgagta ctcctttgat caaaccactt
    27241 cctgccaaga cgcctaaagt cggggcaaaa acagcggcga tcatgtctat aaaagagttt
    27301 aaaatgcctt ttttctcagt tcctctcttt ccactagatg catcatcttc tagtttcatt
    27361 tgtttggata atacgtcata cacttcattt actgcatttc ctactacgac ttgaaacatt
    27421 cctccagctt cgactacagt gatcacacct tctagttgct ctaccttctc cttatctgcc
    27481 ttcttattat cttttagggt aaagcgtaac cttgttgcac aatggtaaac attgctgaca
    27541 ttttcttcgc ctccgacaaa tcggacaata tctttcgcta attgtgaata gtccattttt
    27601 acttccccct tgttaattaa tgccgtagca atccttctta caagataagt atagttctta
    27661 gaaaacggtt tctccattca attagtaccc cttctaatag cgaacgaaac tgtacaatag
    27721 tgtgtatggt ttacttagcc gagcaaatca ctcactgctt gaaagaaaac aagtatatcg
    27781 tttctcttat agtcaaagga gacaaaaaaa acacgcttga tcttcctttg ctgatgtata
    27841 agtggtatct caagctgata ctctgtgatt tttgtttcta cttggctttt gataagacag
    27901 cacaacaaag tctcggcatt taacggaagc ataactaggt cagaactagg agagattagt
    27961 tgctgaatgg aatctatctt tttatggatt tttatttgat tttcttcttt gtagtaatga
    28021 tcaagcagtt ttgttttctt tttttcttca actatggtca gcttttcacg caaaatctga
    28081 taaatctccc aagattcaaa ctcatctaga acactaggta gataaatcaa atcatcccac
    28141 tcaaaaataa gctcttgctc agtagtcaaa attgcatcat attccgttat tttgtttgat
    28201 tgatttttga aaagatacac tggttctatc cggcattctt tgatccacat gttcatactt
    28261 tgttgaatct gtttttttag tgtattcaaa atactaattg gctggttact aacaatcagt
    28321 atctcccctt gttttcgaaa aggattcaac gcttcggcaa gatacaaaac aaaataagcg
    28381 ctttcagcta aattgggacg taattgaaaa tgtattggga agaaacgccg aattaaataa
    28441 atttctaaag gataacggga gatgatcttt tgtacaaaat gattcatggt attatgtccg
    28501 gcatccatcc tcgttttcat ttgtttgata tgggttagca aaaattctgt ttcatggaac
    28561 aataacgaaa caggaagatc taaaaaacga ataatcgttt gctcaaatgc tcttagattc
    28621 ttctctattt ctttgttttt ttcatcatta atcatcaaat aatttaactc taatagtcga
    28681 ttttcaattg cttgtttttc ttgattggaa aaagaatagg gtagttctga tgataacttt
    28741 tttgtgaggg tctctaccat cggaataaat aatgtatacc tgtttgtttt ttctaaaagt
    28801 tcttctcttc gaagaatggt caaaacgaga taccgagaaa atcgtatgaa gtcctctcct
    28861 gaaacaatat aattatatga tataagaacc tcttttaatg tcttttggac agtcggcatg
    28921 atattggaaa aaattgtact gatttgttga ggaaaccttg cgtgttggat caccgtttct
    28981 gttccaacaa gagataagaa aattcttttt gaattttctt cagcaatgat ttttagtcct
    29041 tttgtcgacg aaacttccag ttctaattta ggattgcgct cgatccaacg caaaatcgtt
    29101 tttatttgta atgatacggc tgtttttgaa agaaagaaaa tgtctgctat cttttgcata
    29161 ctgacatacc ctttttgaaa caataagtac gtcacaatag ttgaagctcg gaaatccatg
    29221 ctggaataga ttttatgttt cgtcacctct tgtatgattt gctgtttagt ttcagattca
    29281 attgattcta aaaaataacc tgaacgttta tttgactgga taatgatttt cttctcgtag
    29341 atagcaagca tatttttgat ttcagtttgg atcgttttct tgttggtacc taaagcttca
    29401 gctaattctt cgcttgttat ccaatgattc ttatttctta atactacaag aatttggagt
    29461 tgtcgtttat ttaagtccat ttgcttacac ctcctctttg ttataaacat acaggaaaaa
    29521 cctctcaaat gacaatagtc acttgcagag gtttaaaaat catttgaaga aagatctata
    29581 ttcaaggttt ttaccattag actggataat ttgtttataa tattcaaaac tatctttttt
    29641 caatctccta aagcttccgc tcccatcatt ttgtctatcc acataaataa agccatatcg
    29701 cttcctcatt tcccctgttc ctgccgatac gatatcgatt ggtccccacc ataagtaacc
    29761 aatgatttgg caaccatctt ttattgcctc agcaatttcc tgaagatgta tttttagata
    29821 ctcacaacgt tttggatcgt ggattcgatt caattcatct ggtgcttcat ctagtccaat
    29881 gccattttca actatgaata atggtaattc ataacggtct gtcaattcat tcatgacata
    29941 acgaaaccct agaggatcaa ttggccaact ccaaggttcg ggagattttt ccttcaaaaa
    30001 gggattatcg aaaccatttg ctccgccagt atctacgcgc atttctgctt cttttttata
    30061 gacagcgcta cgataataac taaatgcaat ataatctgcc gtgttagctt gcaagcactg
    30121 ttcgtctcca tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata
    30181 agctggataa tgccctcggc acatgacatc ggagaaaaac catgaattgc gtttaaattg
    30241 cagtgaacca aatacattct ctggatcaca gtcatatgca taagtcgcta atgcagaaaa
    30301 gctacacata atacccatct tagcatttgg atcgattttc tttaataatt gagtagcacg
    30361 ggcgttcgct agaaacatat gatgacaggc ttgataaatc actttttttg ttagtgtagc
    30421 aatgggttca agtttatctc tagcagggaa tgtaaccaat ccagcagctg cataaggcat
    30481 acgaaaagca ttattgatct cattaaaggt catccaatat ttcactttcc cttggtagcg
    30541 ttcaaagacg gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat tttgccaccc
    30601 cccatactct cctaccaatg ctaagggggt ttcataatga ctaagagtaa ttactggctc
    30661 gatatcatat cgatttaatg tattgattag tctgtcataa aatacgagac cagcctcatt
    30721 tggcttctgt tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc
    30781 ttttaaacct aactccttca gtaaacgaat atcctcttca aatcgatgat aaaaatcaat
    30841 cgcttcatga gaaagataat ggagtgattc gtctaaagca atctcccaaa tctttttttc
    30901 ttcattccac tgaatactag gatgctggtt catgatcccg ataagcgtat ctgtcacatt
    30961 aggtaatttg ccgtcttcca accatgcacc ttcacattga ttagcagcta ctgctccgcc
    31021 ccataaaaaa ttttctggaa atatttcctg atccataact atctctcctc gctatcaaca
    31081 atttcttact tgaagtatag ctcgattcat caaaacaaga tattctaact atttcggcag
    31141 aagagaagaa aaaaattgaa tgctactctt catttttaat atttatatat aacaaataca
    31201 taagacgatc cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt tattttactt
    31261 ccaacacaaa tgcttcataa ggtcgtaaat caactttgcc gtctggtaac tgtttcagag
    31321 gataatttga cacaataacc cttaaagctt cacgtggaat agtgaacgtc tgcttttcac
    31381 tggacatatt acaaataacg agccattgct ggtcttcata tatgcgagaa taggcaaaga
    31441 catgctcagg cgtcttttcc actaattcat agtctcccca gacgatgatg tcatgttttc
    31501 tacgcaaatc gatcaatttc ttgtacatat aaaaaacaga atcttgatct gctaacgctt
    31561 cttctgcatt gatttcttta taatttggat tgactcgaag ccatggaatt ccagtagtaa
    31621 acccagcatg ttcacgggcg ttccattgca taggcgtcct agcattgtct cttcctttcg
    31681 ccttgattga ttccaagatt tcctcttttg taaatccaga agaaatcctt tcgttataca
    31741 tattgattgt ctcaatatct tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc
    31801 ctaactcttc tccttgatag atataagggg tgcctttcat catatggagc aagatagcaa
    31861 acatcttggc actttctttt cgatacacat cgtcatttcc ccaacgagaa acaattcgcg
    31921 gaagatcgtg attattccaa aataaactat tccatccttc atcacctaaa gatgtttgcc
    31981 atttggataa tacttttttc agttcagaga tactcaaggg tttcaaatcc catttgtctt
    32041 tgccttcttg ctgatctaat cctatgtgtt cgaattggaa gaccatggat aattcatttc
    32101 ttttaggatt ggaataaagt ttagcgattt cggtagttgc tccccatgtt tcgccaacag
    32161 tcatcagatt tttgtcacca aaagttgctt gattcatttc ctgaagatac tcatgcagtt
    32221 taggtccatt acttgtgatt tctttatccg gttctttgcc aatcatatcg atcacgtcca
    32281 tacgaaagcc cccaatacct ttatcgatcc agaaattcat catttgataa atctcttggc
    32341 gaacctgttt atttttccaa ttcaaatcag gttgacgctt gctatacaag tgcagaaaat
    32401 attgccccgt tttttcatcc aactgccaag cagaaccact gaatattgag cgcaatctat
    32461 taggctcttt tccattgacc ggatcacgcc aaatataata gtcccgataa ggattttctt
    32521 tatttttacg ggcctcaatg aaccacggat gttcatcaga agtatgattc accaccagat
    32581 ccatgatgat acggatattt cttttctttg cttcttcaat cagttcttcc atttcttcca
    32641 tcgtaccgaa ttctgccatg attgcttgat aattactgat atcataccca ttgtcatcat
    32701 tcggagactc gtatactgga gaaagccaaa ttgcatcaat acctaaatac gccagataat
    32761 ccaacctgga aataatcccc cgcaaatctc caataccatc ttgatttgaa tcttgaaaac
    32821 ttcttggata gatttgataa acaactgaat ggtgccacca tttttgttcc atgattttcc
    32881 tcctataaat ggatgtttct attttttgct taatcattta ttttagtctc tctcactaaa
    32941 aattgtaacg ctttcaaata gctattgcac cttgttatcg gtaacagaat aaaacaaaaa
    33001 aacagctgag gaaattccct caactgtttt ttgaaaggat tgatttatgc agggaagaat
    33061 gctgatttca caacttcttt ataaacggtg ttcactcagc tgtcccttgg tattagctca
    33121 aaaaacggca aaatataaga aactattttt tcgttttttt ctctaatact caggccagaa
    33181 cgctgatttc acaacctctt tatttcacat tcaatatcca catgaatccc acaaatacaa
    33241 agaacagacc atacatgatt ggagatactt cttttcctct tttagctgct aacatggtaa
    33301 ttggatagaa gataaaacct aatgcaatcc catctgaaat actataagtc aaaggcatgc
    33361 cgatcaagat caagaatgaa gggatcgcaa tctctaagtt tccccattcg atttgtcgca
    33421 aagattgtgc catcaatact cctaccataa tcaatgctgg tgcagttact tgtgaagtaa
    33481 caactgatag taatggagaa aagaacaagc ctaacaagaa gaaaatccct gtagtaatag
    33541 ctgtgatacc tgaacgtcct ccaacggcga taccagctga tgattctaca aatgcaccaa
    33601 caggagaagt accaaataag gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt
    33661 tccctacacg aggcatcttg ttgtctttca tgaaacctgc ttggtttgct aatccaacta
    33721 aagttcctgc tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg acccacattt
    33781 gaagagaatt gatgtcccct acatgtttta atgcaactaa aaatgttggt ttcaaacttg
    33841 gtgctgcggc aacaatttca gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg
    33901 ttgtagctgc catcccgatg aagatgccac ctggtacacg ccgaacaagc aagatagcag
    33961 ttacgactaa tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat cctaatccta
    34021 ctaatgtaga ttcattcgaa acgatgatcc caccttcgct cagaccaagg aaagcgataa
    34081 atagaccgat accgccagaa atcgcatatt tcaaatctgc tgggatcgca tcaatgatca
    34141 tttcccgcag cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg
    34201 ctaatgctgt ttcccatgga atccccattc ctaaacaaac agaataagca aaaaaagcat
    34261 tgatcccaag agcaggtgcc gttccaattg gataacgggc taataccccc attaaaatac
    34321 agccgatcgc actggctaat gcagtcgcag taaacactgc tccttcatcc attccagaag
    34381 cacctaatac agttggattt acaaataaga tataagccat tgagataaat gttgtaaatc
    34441 ctgccagtac ctcacgtttt accgtggtgt tcaatttttc tatttcgaag taggaaagta
    34501 ttttttcctt catcttccat ccccctaaat ccgaacatta taaataaagt ccgttaaaat
    34561 ttcgatttat taataaaaca acgtgtttta tcatatacta aaacaaaata taagtaaaca
    34621 aaaaattcgg attttaagca aaatccgaac gtttcatctg attaaatccc tttatctcaa
    34681 agttcatgaa cattcacttt ggattacaag aatttcttta ttgtgttaat aaagtttctt
    34741 cattctagtc tcaaaaatca atagtgtgat ttgattttac catttcgttt ctgttattct
    34801 ttgatgaatt cttttgtatc gacaggattc agattattta gtccgccaaa acctgtgtcg
    34861 accatttttc cccagccttt tgtcttcttt tttcctgatt tgagattctt tgctcgaagc
    34921 ttctcactat gttttgctcg ccgatacttc aaataacctt ctaatcttcc cattagatcc
    34981 actgtttttc ctccttttta ttcaatcaga cgactcagct tttcctataa caaaataatt
    35041 gccttcaggg tccgaaaaat tgaaagtcaa gacgccattg ttgtctgtga tttctcctgg
    35101 ttcttccatg ttctcgtaga tagtctcaaa ttcgtccgta aaaaacacta aagaaggcgg
    35161 tggtcctaat acttctggtg aaaatttctg cacaaatact tttgggaata cgcctaattc
    35221 taattctttt gagatgctga gtacaatact cttaaattct tctggcaatt cgattgtttc
    35281 agaaatttct gcaccaattt tttctacaaa gaatcggcaa atcatctcta cgtcattgac
    35341 ataaagcatc acacgtgttt tttgtatcat cgtttgaccc ttctttctat ttgattttct
    35401 ttcctgtcta taaactgatt ccattatatc acgaaagcgc aaacaccttg tgtttgatac
    35461 tattcaagaa aaataaagca atagtttcta ggattgcttt cctccacaaa ctttttttac
    35521 aaaaaaaaaa aaacggcaat gaatgccgct cttctttttt cgattctttt ataattctgc
    35581 ttctgattca tctttggtta gtgtgtgtct aacactagtt acgccaaatg tttgatcgat
    35641 tttttccttc aacgcttcga ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga
    35701 ttcaatgacc agcaccgctg cttgtgtttc ttccgtcagc atcgttcttt gggcttcacg
    35761 ccagttgaga cagcgacaga tcgctccttg ttcatcatag tagatgattt cttccggaag
    35821 tgccggagca tcggaatctg cacctaaagg caggaatgat tcgccacctt gggcttttcc
    35881 taaatgcaga tttcctgcga tcttttcgat atcttctccg ccgcaaggta cgccaaaaga
    35941 caaggaaata ctattataga tatctacaag cggattgatt gggaaaaatt cccgttcttg
    36001 atttgcacgt ttcagtaaag cttcaataga agaacgtgca ccttttttag tcttgaatgt
    36061 ggtaaatgcc tgacgccact gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag
    36121 gtaatttctt gcttcttttg ctgcctcatt caataattga tgatacgttt cttcactttc
    36181 ttttgttacg tggttatcga taccatttac ggataagatt tcgatggttg cttcaggaaa
    36241 tacttcccag aaattggtat ccactattac ttttttcata tgttcacacc tcttgattag
    36301 aataattgct tgatgaatag atagtctgtt tggggatcat ctcccatata aaaaacatgt
    36361 tcgctgtatt tgataaatcc ttttttacga taaaaagcga gcgcaacatc gttgtgttcc
    36421 caaacaccga gccaaatggc tgttttttgc ttttcttttg ctttttcaat agctgtatct
    36481 agtaaaaatg taccgatacc ttgatgttta aatttttttc gtacatagat tcgctcgatt
    36541 tctaaaaaat gatcagccat ttcctcagat tgactcgttc ctgtatttag tttcaggtaa
    36601 cccgcaggct cttgatcgat actcactagg aaaaaatcgg tttctttatc ttttagctct
    36661 tctgtaagtt tttctggcgc ataagcttgt gtcaagtaat cagctaaatg cttcggtgaa
    36721 ttatcttttc caaatgtatc tgtaaatgtt tcgatactga tttgttgtag taagtctaca
    36781 tctgaaggcg tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc
    36841 cttttattgc cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt
    36901 aaaagatatt ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg
    36961 gagtagttgt tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag
    37021 agtttcttga tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct
    37081 aatttcttga ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt
    37141 tcttggatga ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt
    37201 aaatcgactt ctttgaattc gatattgctg atagaatcta gcgctcgggc aatcattccg
    37261 atgtctcgaa ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttta
    37321 cctctttttg ttgcaaatgc aactattttc aataaaaaaa tcaagttctt ttttaataac
    37381 tagtaaagta tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt
    37441 tgttttctaa aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact
    37501 cttctatact aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt
    37561 caccgtggat cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt
    37621 tttgtttatt cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt
    37681 cttcatagac ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac
    37741 caggaagtga ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt
    37801 tgatataggc acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg
    37861 cttttatttc ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc
    37921 ggatctcctc aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag
    37981 aaagtgcgag tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt
    38041 tagcaaatgt atgactatgt aatcaagtat agaagactag tttaattacg gcaaataaat
    38101 ataatgaaat ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga
    38161 cttctctggg gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca
    38221 ccaaacgaaa aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac
    38281 agctttttca caacctcccc actaaacggt gttcaatcag ctgatgctcg gtattcacta
    38341 aaaaaacggc aaaatatgag gagctatttt gccgtttttt tctataatac tcatattaaa
    38401 acactgactt cacaacctct tccttaaacg gtgttctcct accaactcct cagtcacaag
    38461 tcacaatatt catcaaacat gagaagctgt ttcttgaaat tgctccttgt gactcggtgc
    38521 tgaacggtag gctcacgacc tctcggtaaa cggcgttcaa aagccaattt ttcgatatta
    38581 agccatttct ttgttactta ttgtcattga ttttttttct aatttatcca atagtggagt
    38641 gtaatccact tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt
    38701 gtcatatttt cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc
    38761 tttcgttcga tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg
    38821 acgatctgat tcacgatcac gtttgtaccg acgttgccat aacacttcaa agtctgctgt
    38881 taggcgaatc gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt
    38941 ttgcttatca ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa
    39001 atcaagtgct ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc
    39061 caaattgtta aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc
    39121 atacatatct ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg
    39181 actaccagcc agaagaatga aatacttttt catctcattt atttcctaag ttaattggac
    39241 tttccaagtt cataaacagt ccaatcttat gcttgacaat ttcttttact gcttcatttg
    39301 ctggaatcac attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag
    39361 ctactttcgt ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat
    39421 tgatcaattc aggataatct tcttctttca cacctgttcc tccatggata actaggggga
    39481 catcaactac tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga
    39541 attgtccatg attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa
    39601 atttcactgc gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt
    39661 cttctgtacc gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat
    39721 attctactac tgcttttgtc ttcaggatat tttctttgaa aggcagatgg gacccatcat
    39781 acatgacaga agtatatccg ctgtcgattg cttctttgat atcatcaaag tctcgggcgt
    39841 ggtctaagtg caagactgca tcaaccattt cttcttctgc aatagctgtc attacattga
    39901 ctaacacatg catgctgata tatttagcag tatcgacact tgtttgaata atgattggcg
    39961 aacccatttc ttttgctgcg cctagcatgg caggaagcat ttctaggtta tgcgtattaa
    40021 aagctccaac tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat
    40081 acatctttta ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca
    40141 atttgtcgca tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca
    40201 cgagcagcat ctgaacgctt ctggttatag aggctcatca gttttttata cccatctccg
    40261 atcgtctgtt tattttccaa gctcttttct aaatattgga tggcttgatc gttctcatac
    40321 aattcagaaa atttaagtcc aacttgttca taaagttccg ctaatttatg cctatcttgg
    40381 gtagtggaaa tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct
    40441 ggcgataggg ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta
    40501 aaaaatccaa acatcatttt ccctactttc aattaaaatg ctttcaaaac tgggcttaag
    40561 atccaggcat ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgatg
    40621 aatcccatcg aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca
    40681 tgagcaatac cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct
    40741 gctgtccctc cggcaaagaa attggttagc ataccagata aaatcatcgc taagccgaac
    40801 atgggcaaag taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg
    40861 acagcatttg ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga
    40921 actaatttca tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca
    40981 ccagctagca atacatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg
    41041 atatagtttt gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg
    41101 atgatatata gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg
    41161 aaatgtgtcg gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa
    41221 agcttagcga caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata
    41281 tcatttgatc cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg
    41341 aaacaagctc cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa
    41401 tagccaaaga ctgtcgtcat cgttgccatc cataaaattg cttgaccatt gatagattca
    41461 atagaaggga tgatcccttg catatgaaat ccttcagtaa agattttccc gaaataagtt
    41521 aaacttccaa cgatgatgct cgaaccggca ctcaacactt gaaatcctag gagtgtcttt
    41581 aacgtgccag agatgacttg tcctgctgat ttcttctgca gtagtagacc caacatagca
    41641 atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt cgatgataaa attgataacg
    41701 ctcatgtttt taccctcttt tctttttgta tttagataac tcccttatct tgcagaactg
    41761 gggtgatttt ttcttcaatt tcattttttg aaacaatatt tttcaaatag atcatcgccg
    41821 ttttaggatc aactgcgaac ttttcgaact gcgaacgaaa attttctgct gtgatgatca
    41881 catctggttt catggaaact gcggaagaaa tatcacaatg gtccagtttg gcttctactc
    41941 ctaatttgtt caaaacatcc tcagcagaca tctgtgctgc aaaactactt cctaaacctg
    42001 cgccgcatac gaataaaatg ttcaatttgt tcatctttgt tctcccccgt atcatttatt
    42061 atttctcaac tactgaaaaa agcagttgtt tgaaatcttc gatatttgtg cattcaatca
    42121 atcgatccaa cttctcttcg ttgttgatca attcaattag ttctttcatg atgttcagat
    42181 gcgagtagga gtctactgct gccaaacaaa aaataatctt tactggatcc atttcaggat
    42241 taccgaaatc gattggttgc tcaatcgtag ctacgctgac tcctaattta ttgaccccat
    42301 cttctggtcg ggcatgggct agtgccatgt gtttgccaat cacgatatac ggtccgtaat
    42361 cattcactgc acgaatcatc gcttcgacat agcgctcttc gacaatgttt tctttaacca
    42421 atggtctagc agctagttca atcgattcct cccaagtagt ggctttttct ttgatcatga
    42481 tattgctgtc cgtcaaaata tcttttaaca taggctggat ctcccttttg ttgatttcta
    42541 aattgtttgt ttcgaataat ttttctaact tgccatagat gattcgatct actcggccac
    42601 cactttcttt catgacatct attatttgag tgaataactc cgtcgattca tgactgttag
    42661 gctctaaccg ctgtagttct tcatttcttt tcaaaaagtc ttctataacc aagatattcg
    42721 tctcagttaa aatcggatcg acaactaaaa gcggcttatt tcgataattc aattgaaagg
    42781 tgctgaaaac cagattgaca tccaatttat ctactaattc gatttctttt gaacttaaaa
    42841 ctgcagtaat ctcgatctga ggaaactttt ctttcagatt ctctgctaac agatttccag
    42901 tagccatccc atggttacag acaacgactg atttataaac ataattcaag tctcgcttga
    42961 ttacactggc gatcgtcaaa aagtggatga caagaaaagc tatctcgtcc tccagtatct
    43021 tgccaccaat tacttcttcg atcgtaggaa caaaatgagt gactgcgcgg tagattggga
    43081 aataatttgt tcggatgttt tctttcaatg gattcatgac ttggatatga ttcttacaat
    43141 cagtgcagcc atgtgcttat agagattttc gaataaagct tcttccttta gatggaaagg
    43201 tacgtgtgtc tcgttctcta caaattggat caattggatc gttagtaatt gagcttgtac
    43261 ccactcgatt gaattggaga tatctctagc attgaacgtc tcaatagtga aacgaagata
    43321 atttctttct acttgcggga cattgtccaa ttgaaattcc tcgatcatgt ggtcaataaa
    43381 tgcagaaaag ctactgtctt ttatttctgt atttttccaa ttaatggcag agataaattc
    43441 atgtttcctg attcgctgga tccagatcaa tgtaaaaagc aggatttgtt ttttataaat
    43501 atcatcttct tgtttcaaca acttattgga atagatctcc tgtatttttt tgatttccac
    43561 caaaggaaaa taatggagaa agattttttg agccggtgtt aattgtgcat cttgattcat
    43621 aaagaagtcg gtcattccca gattctgatt gataaaatca tagatcatcg tacggataga
    43681 tcgttctgaa cctttataaa ccagtccttg ctttccataa ctgacgatct ctatcccata
    43741 actgaccagt gttgctcgta accgtctcat atcttcatct aatgtacttt tagagatttg
    43801 aaattcttct tcttttttgt ttagatggat cgggattcgg ctgtaagcaa cggctaaaac
    43861 tagatcaaaa atacgttcgc gccgattcaa aaagccgtct ttcacatctt ttatttcagt
    43921 ttctaatcga gcttgctttt ccttttccag cttcaacaaa aaacctttgt tgcgaattgt
    43981 acggatctcc ggaagctttt tactgctcaa ccagatattc gcttcaagaa cttcctgacg
    44041 gatcgtccgc tgactgacat tgaactcact tgctagatgt tttgctttta ctggatattg
    44101 ggctgtcatg attctgtgaa gtatttcaat tgttcttgtt ttcatagaaa tgatctcctt
    44161 ctgatttcaa gtatatggtt ccgcttacat aataaaaagt agatatttct gccacttgaa
    44221 gtggaaagaa agtaagcaaa gcaaacaaaa aataagccat atgctttttc atctcctcaa
    44281 cgaaaaagac atacgcctat tattacaaaa attttgccca tccatacaaa aaagaggttg
    44341 tgaaaaagct gtcctgcatc aagtaataag aacaactaaa ctaaaatagt gcttcctatt
    44401 ttttgtttgg ttgttcttat tatcgcagat gcaagctttt gaacaccatt tagtggagaa
    44461 gttgtgacag agatgttcag ccttgagcga taagaagcga ttttgaaaaa tagcttccca
    44521 tattttacaa aatcgtgcct tattgccgat aggctatctc tggcacaccg tttattcaga
    44581 aaaaggagtt gcgacaaggc ttttgtcaca actccgttca taaatatttc ctgtcaatcc
    44641 agcataatcg gctgaataaa atcggcttgc catctttttt ctttctcaga aaaatcaaaa
    44701 taccatatct gagaaaaata ctgtcgttgt gtcttacttc ctgagatcaa tcgcccatct
    44761 tttcgatcga ctgaataatc acagcatctg aaatcaattc ggatggcaaa actattttcg
    44821 tggatctgtc gataatttcc tacttcctga atagttattt ttttaggtat gatttcttac
    44881 accgtctgct ttgttttttt gcagaacttt ctgattttcg gcaaacagct tgtctgtata
    44941 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
    45001 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
    45061 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
    45121 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
    45181 tgtacttgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
    45241 gcccccatat gaacgaccgc caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
    45301 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
    45361 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
    45421 ttacaaaaaa ctatttttat catagccttg ctaaaaggaa gagtcaataa ccagatgcat
    45481 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcga acagatgtat
    45541 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
    45601 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgaa
    45661 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
    45721 ttctaatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
    45781 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttggattgca
    45841 agcctgatat gcggataccc taatattgct tcttcatatg caccatttac gccatatttt
    45901 tgcttcaccc tatgaccatg agtgtttttt ttatgacgat attttgtatc aggaaaagca
    45961 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
    46021 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
    46081 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
    46141 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
    46201 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
    46261 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
    46321 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
    46381 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
    46441 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
    46501 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
    46561 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
    46621 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
    46681 atgactaaac tgtataaact ttggatcgta ttcaagtaag gcgcattcgc aaagctcaag
    46741 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
    46801 aagaaaagat aacgtactct gtccaaatat tgataaactt tttcatgata aacaaatgcg
    46861 gctaccccat aaatcccgta aatcatgaaa ctagcaacaa accgatctaa caagtaccag
    46921 ctgtcatgat aaggcccagt caatacttgt gtttgataga acagatacca tgcgacatat
    46981 aaaatcgttg aaccaatcaa tactggccat acatattttt tattagcaaa tactttgtaa
    47041 ccgagccaaa caaaaaacgg catgaacaat tggatctgca gcatcataac ggtataccat
    47101 aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa agcttgcagt atcgtatacg
    47161 gagtttgttg ttggactgct ggaaatagcc aaagataagc cgtcgtccat agaagatacg
    47221 gaacgactag ttcaaaaaac ttttctttca aaaattccca ataagacaaa tgctggcttg
    47281 tttttaccat attataaaca attgcaaaaa taaacattgg tgctgtgtat ttaacgatga
    47341 cgtaaaaggt accaataaaa gctgtatttg ttgcgctgtg gctgtttgcc aaagcaaagc
    47401 tcaatatcgt ttgcatcata acagccttta cagctgatac tttctggaag tcacttacgt
    47461 tgattttgat ttttttgttt tcaaacatct ctttctctct ctttttcttt attaccaaag
    47521 ttctttagtt aatttaggtc cttcgactgt tttcaataga atttttttct ctggctctct
    47581 caaatactct tttagtgaaa aaccgccttg ccgagtcact acttgggtat cgaattgctc
    47641 aaaaaaagca ggatgcgatt gaattacctg aattggcaat tcaatcggtg aatttgcgta
    47701 cagtagcaaa tcaaaatcac tggtttcttt tactgccgga atcccagtcg ataattcaaa
    47761 tcctaagctg ccgccgattc cccatttaca tcctgataat atgtttctag cagcatgaaa
    47821 ttgctgataa acaggataat ccatgatttt ctcttgtcta tacgattctt gttcgatgat
    47881 gtcccacgga tgaatgactt gtttgacggc atgttttgag atttctgttg cccatctctg
    47941 attgcgggca ggtccacgta aaccaatcgg tagtatttct gcgtgatccg tcgacgcacg
    48001 acgaactgtt ccgaacatct tttcagttag aacgacccat tcaggcaagg tgaacccttc
    48061 tatttcatgt ttatcaaaaa ggacaatatc atgtgcttga atcatttttt atgcatccca
    48121 ttcttcgtcc attttggcac ggactttatt tgtttcgaca cgtccgaatg ttttggctgt
    48181 ttcgtttgtg tatcgatttg tcagcatcgt gtcatttgta ttgcggacat cttgaatggc
    48241 ttcattgatt gcttcgatga ctttgctgac attttgttct gaagcagctt ggtcggtgat
    48301 atcttctagg aaatgataga tcgctcctag ttttgtataa ttattgatat cgtaagcgat
    48361 agctggaact ttttcagcag ctgcttcgat ttcagcaacc gaacgttttg tcacacgtgc
    48421 agcactttct ttagacatcg cttggacggt aatttcttta ttgtttaaag caatcaagcg
    48481 gcttgattgt aatccatgtg ctaaaaatgc acctgaaata gcatttcctg caatcaatcc
    48541 aataactgga tatccagctt gacggaattt tgcataagca gcagcactat tagccaaagc
    48601 cacatggata ccgattaatt cttcgttata gccgtatgct tggcttggta catcgatcac
    48661 aacgatgatt ggccgtttca cttttttatc tttatcttct tcgtaaacgt gatccaaaat
    48721 ttgtcccatg cggaaacctt caactaaacc aacttcaccg tttcgtacac ggtacatcgg
    48781 gtttttagga tcaggtacga ttgatatcac gatggtttct tctatgaatt tacttgattt
    48841 accataatac acagtactaa tagaagaagt tggatttttc atgccagtta gtgccgcaaa
    48901 ccaatcgtaa ccaacacttg ctgcagctgg ttcatttcct tctgctactt ctggtaagtc
    48961 aattgatttt gcttgatggt tagcaattgc ttcattgtaa tcgtcaatgg ccattggttt
    49021 tgaaagatct aaagaatcaa gtaatgataa ataaaatgct gagctttcac tgcgatgcgt
    49081 gtcttttcgt tcgtccattg ctgcgatgac agcagctttg attgcttcta cactatcagt
    49141 cactaattca tcaatgattt ttgactctac ccgttgacga gatcctaatg tattccaaat
    49201 aaagtctttg tcactagaat caaattctcg aacgcctgct tcttgttcaa taacttctgg
    49261 tccattcaaa ccgactctcg ctttttttgt tgcgattaaa taactcatta atgctgaagt
    49321 aatcgacata ccgccaaatg atcctacacg tcctggaatc aaaccgatga ctggtacata
    49381 ttgttttaga gcaatgatta catttccgat ttctgaaata gataacagtc cgtagtttgc
    49441 ttcttgcaaa cgaacaccac ctgtatctag tacaatgact ggaaagatct catttccttt
    49501 tttattttct tctaacacat gttcaagtgc agcaacgatt tttgcaccgg aaacttcacc
    49561 gataccgcca ccttggaatt ttccttccat agcgattact actgcttgtt tgccattgat
    49621 cgtgccacgg ctgacaacga tcccgtcatc actttcaggg acgattcctt gtgctggcaa
    49681 acttggcgcg atgatttgat cgaatggatc aatcaattca cgtccattgt caagtagagc
    49741 gttcactcgt tctctggcgt ttaattctac aaaactattt ttcatctgaa agttcctcca
    49801 ttgcttgcgt caatcgtagg tacacaactc ctggtgtagc accgaagtca ttgatcacga
    49861 tatctgctga aattggataa cgatcaaaaa atctcgttaa tacattgttc catacttcgc
    49921 cgaagccatc gcttcctgta caaattttta cagtagattc tgttttttct gttggaaaca
    49981 tcaagatttc caaatctcct gaaccgacca ctcctacatg gattttctta ttgatcggct
    50041 gtttcgttgg atatttataa gctaattgtt ccatggatct ctcccctttt tgtaaaacag
    50101 agcatacacg ttctgcttat tcgtctttct tcttccatct gggtaaatgg cgacaaaagg
    50161 tttttctcgc caattttatg accaagttct gaatttcatt ggtggttcat acaggccatc
    50221 tgaccattcg atcaaatcat cgattgtttg tgcagctaat aatgaacgtt tcgcttcact
    50281 gcgtttgata cctagatctt ctggtaattt cacgatgccg gcttcacgca agctatctag
    50341 tgattctttc gtgctagtca ttccaagagg tgtaacaccg ccaattgctg caattgctgc
    50401 ttgacgttct tcttttgttt ttgctttgta caaataagca attccttctt ctgtcacgat
    50461 atgggtagta tcttcgctat agatcatcac tggtgcattg gctaagcctg cctgtttttg
    50521 aacaactaat gcatctaatt cttcgacaaa gactggtttt ttatttgaac catatgtttc
    50581 aaccatttga acaactaatt tttgtccttt acctaatgga tcatcattgt cgcgaagtga
    50641 ttgccatgct ggtgtagaat gacgacgacc gccaggatta tgtcccatgt taggcgctcc
    50701 accaaaacca gataaacgtc cttttgttac ggtagaagaa tttccttcgt aatctaattg
    50761 caaggttgaa ccgataaaca tatcgattgc atattgtcca gctgcttgtg ataatgttcg
    50821 gttactgcgc atcgtgccgt ctttaccgac gaaaaagaca tctgggcgag cagcaatata
    50881 tttttccata ccgacttcac caccgaaact atggatactt tcaacccaac ctgattcgat
    50941 tgctggaatc atcgttggat gtgggttcaa tacccagttc ttcgcgattt tcccttttaa
    51001 ccctagtgat tcaccataag ttggcagtaa taattcgata gctgctgtat tgaatccaat
    51061 accgtgattc aaagattgta cgccgtgctc tgcgtagatt cctttgattg ccatcattgc
    51121 cattaggatt tgcaattctg tgatgttttg aggatctcgt gtgaataaag cttctaattg
    51181 ataaggttca tctgctttta cgacaacgtc tacccagtca ccagggatat ctacacgtgg
    51241 taaagaatcg acaacttcat tgacttgaac gatcacgatg ccatctttga aagctgcagc
    51301 ttcgacgatt gttggtgttt cttccgtatt gaaacctgta tacaagttgc cgtctttatc
    51361 tgctttatct gcaactacta agacaacgtt tggtatcaaa tcgatgaata agcgtccata
    51421 aagttccaaa taagtatgga tatcacctaa tttcattgtg ccgtcttcaa tcatttgggc
    51481 gacacgcaag ctttgtgcac ctgcataaga gaaatcgatc ttgcttgcga ttcctttttc
    51541 aaaaatattc aaatgttctg gtcgtgagat actagacatg atcaaatgca gatcattcac
    51601 tttttctgca tctacttttt caagactttc tgaaagaaaa cttgcttgtt tttgattgtc
    51661 gccttctaat acaactttgt cgccgggagc aatcagtttt tccattactt ctacgatttt
    51721 ttcagtttct acatacttgc cattgagcaa gttgctgatt ttttccaatc gctgcgcttt
    51781 tttttcgcgt ttagaagccc agttgcggtt tggtttttcc ataattagtc atctccattt
    51841 ttgatataga aacgtggcgt catcaatacc atgttggtct agattgatcg tacggatccc
    51901 tttatctaca ccaaattttg tatgaatcag ctttgtcaaa gtattgccgg ggggaaattc
    51961 aatcgtcaga tccattccta attcttttgc caagtccatc atttggttcc actgcacagg
    52021 gaaagctaga ttttccaaca aatctttacg aatctcttct gcgtcctgag tggctcttcc
    52081 atccgtattt tttatataca aacatctggc atcagataga tggacagact gcatatacgg
    52141 cttgaatttc ttgaccgtat cattcatcaa gagacagtga gatggcacag ggactttcag
    52201 cagcttcgct gtttgcgctt ggtgccgttt ggcttctgcc aacgtaacgg ataaaccttc
    52261 gatactccca gataacgtat gttgcatcgg acaattttca tttgacagat aaactggatg
    52321 gtcaggatca aatgttttgg ctaccatttc ctctgcttcg cttctcgtca cacctactac
    52381 tacgcccatt gcataaccgc ttggatatgc ttctttcatc aagactgcgc ggtgatagac
    52441 tagttccgct gcattctcaa atgtcaaaga tcgacaagca acagctgcac aaaaagcacc
    52501 gatcgaatgt cctgctgcaa tatcaggctg aataccattt ttttccattt catctaaata
    52561 aaatactgct tttgtcagta atgctagctg gataaaaacc gtatcttgat agttttcttc
    52621 tttatcgact aaccgatacc ccgtcacatt ttctaaacgt tccatgtaca tttcagggat
    52681 atccgctaac atccctaact tctgactgcc ttgtccagga aataagaaac ctgtactcat
    52741 gagcctcgcc tcctcagcac ttactttttc cactgctatc ttagtcaatt tctgctataa
    52801 taaaaaatgc caaaaaaaga aaaaaccata acaaaaaggt atggatggga tggataatta
    52861 tgaaattgct tcatctgcag tattttaaaa aagtattcga aacaaaaaat gttacccaag
    52921 cagccaaaga attatttatt tctcaacctg ccttgagccg tgcgatcaaa catttggaaa
    52981 acgaattagg tgtccctttg ttttaccata gcggcagaaa cattgagacg accgtttatg
    53041 cagaagaatt ttatccatat gcagtcaaga cattatacac attagaagaa ggaatcaatt
    53101 attgtcttcg gtcaatgaga agatcgttac ttccgtcata ttgtatttag aggtagcttc
    53161 tgtttctatt ccgaacttag tccgaatttt tatggaaaag catcctgata tccaattaac
    53221 gatcatgcaa catcatttac cagatgatat taccgatcct gtactgtaca tcacttcgga
    53281 gatgaagcct ggcatgacca atattcctat catgaaagaa cctgtttatg tcgccattcc
    53341 taagaaacat cctcttgcga aaaaagaaca tctgcaatta gaagatatcc agcgcacccc
    53401 catcttgatg ctgtcaaaga gcaacgcctt ccgtcataca ctggatgcag cacttgaagc
    53461 gaaaagtatc gaattgacga tcagttcgac gacagatgat cctgcgacat tacgttctat
    53521 tgtcaagcaa ggattagctg ttagcttttt cccaaaagtt tcttggtctt acgataaaaa
    53581 tgatcctttc gtcttgcggg aaatcaaaga tttgcccttg acacgaacaa tttatttatc
    53641 ttctagcttc acagaagatc atcctttgat caaaatgatt gcaaaaacga tgcgggaatt
    53701 ttatttagga aaataaaaaa aaaagagctg ggacaaaatt cttgtcgcag ctcttttttc
    53761 gaacaaacgg tgttccagaa gtaatttctt agaaaataag ccgaaatttc ccaaaaattt
    53821 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc taaacatttc tgtcacaacc
    53881 tcttctctat tgtcctagac attcattgcg ttgatttaaa acttccatca atcaaatctg
    53941 ctagttcatc tagcttttct attttacgta gatcaataga gatccaatgt tctttattca
    54001 tgtgataggc tggaaaatac ccctcttttc ctcgcaaaat tccgccaagt tcaggatcga
    54061 ccttcaaatc aagaatattg atgatcctat ggtcacttgc atcatcatag attttttctt
    54121 ctggaacaga catcaggacc ccaaaccatt ttttgctttt ttgatgtctg aatacgcaat
    54181 aatcaggaaa ttttttaaaa atatattctg gagacaaatt gtatgtttca ttgatgtgat
    54241 cgattagttc ttttttcgtt agcatctgct tccatctctt tcttattgat tcgctttatt
    54301 caaagatacc acgtaagaat agaaaaatga atacaagtag ctgctaatca aatacttcga
    54361 tatttttctt ttttatcaac tcttaaatgt tcaatccgcc tgtgttggtc ttgctatcat
    54421 ctactattaa aatgttgatt attgtttgcc cacctatttt ttgattttag tggtatcatt
    54481 tttcaagttc atgatgaaat tcttatcatc agtaaatgct ccagaaaaat aaacaatgat
    54541 tgaaaaatca gttgaaactt gaccaccgtt tattttctcc gtattcttag aatagaaggg
    54601 agtctgattg atgtattcac taatgaaaaa aatcattacc gaaaaagata ttcgacgtca
    54661 tgtttcttta gtagaacaat tgctgaatca tacaaagata accgtaaatg aattagctga
    54721 aatcataggg acaaccgaac gaacgattta ttctgacctt caatctattc gctctcaact
    54781 gcctgaaggc tgggacatca ttagcgatca agcagggatt agtttgcaaa accagcaaaa
    54841 tttattagca aatgaccttt gggagatttt tttcaaacaa tcagtcagtg tcgaactatt
    54901 gaaaagtctg ctttttacaa aaaaagttgc tgtccctgat tttctagccg accatgggct
    54961 ttcttatggg acattgaaaa gacatgtgac taaaatcaat cagcgtttag caagttacga
    55021 cttgcagatc gatttgacga aatataccgc ttctattctt gggaaagagc gtgcgattcg
    55081 cactttctat catcgactgc tgattccttt tactcataac aactattttt tcgaagatta
    55141 ttcgattcat gaatcccatt attttcaatt tttaaaaaca ataaatcgaa cggagctagc
    55201 ggttgaaaca gaagagattt ttggaacatg ttggttcttt atcaatacga tccgtataaa
    55261 agcgaattgc cgattagatt cttctatcca tatcaacagt acactctcct ccctgtatga
    55321 ctctgcattg aaaaaattgt atttaacgga aggtatttat ctcaaagaca ctgagctttc
    55381 ttttgcttct ttctgctttc tggaaagttg gaattacaac aataactatg gacaagaaac
    55441 cgcttgctgc ctgcatcatt ctccattttt cgaacgcctc gaaacgtttg tagaggaact
    55501 agcttcagag ctgtctcttg atcaactaaa aaaacatctc taactgataa cttggctctt
    55561 ctcgtattaa aatatcatga atcgccgata ttgattgaag cattggaccg tcaataccat
    55621 tttttttaga aacatatgat cagcaatatg cacatttgtc tcaacagaaa gagaatttac
    55681 tgaacgatct aaaaaaagaa atggaaatca atgaaccgaa tttcttcctt caactcttat
    55741 ctttactgat ccaacaaacg attttttcta tcaaaccgaa tttgttcaat gtctatttta
    55801 ttttccaagg agaaccttca tggaaggcat tctttcagca ggaattaaaa gattacctgg
    55861 gaaaacgtgt ctgcttctta cctatcgaac taaccgatct ttccactatt tctttcgaaa
    55921 aaacagatat cttgatttcc aatttcccgc tggattctct agatatccct atcgtataca
    55981 tttcttctat tcctacgaaa aatgagctca atcgtttgac tgaactcact ttcaaaactt
    56041 ttttatagta acggctagcg actgtattct ctagctgttt tttctttttt gcgaaacagt
    56101 ttattcatga tccctttttc tatttctaca actaagaata gaagaatcac atttgctcca
    56161 atcaataact gttgtgtcat cgttaaactt actgtaccga tcagctgctg gccgactggt
    56221 agaaaaataa ccatcccttg caataaagca aggataccta atgaaagaaa aagcgctttg
    56281 ttttgcaaaa gaccctggtt caatgacggc ttggacaact ctctgcaatt gatcatgtaa
    56341 acagcttgag ctaagacgat attttgtaga agaatggttt gctgtaagct ttgcccttca
    56401 aactgcatag ccaataaata ggcaggaacc atgatcagta gggagacata gatgatccga
    56461 aacaaatgat aaccagatag gattccttgc tttggatctc ttggtgggcg cttcatgatt
    56521 tctggatctg gcgactcgaa ccctagtgca taagataaag tgattgtcgt taccatattg
    56581 acccataaaa tctgtactgg gaacaaaggt agcgggcgat tcagcataag cgcccaaatc
    56641 aagatcaatc cttgagctaa tgcagttggc aagaagaaat tgatggtttt ttgcaaatta
    56701 tccaaaatcc gccggccctc ttttacggct tttgcaatcg tatggaaatt atcatctcct
    56761 aaaaccatat cagctgcctg tttgcttact tcactacctt tgattctcat agcaattcct
    56821 acatcggatc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat tccaacgatt
    56881 tatccatttt tctgcaaagc tttaacaatt cgcagtttat gttctggcgt cgttcgagca
    56941 aagacatcga ctattggcac ttgctgaatc aactcttcat cagacataag atccaaatcg
    57001 atctcttcta aaactttggc agtatgtttt aagccaattt gttccgcaat cgcttttgct
    57061 gtgtctttgt gatcccctgt aatcattttc actgagatcc ctgcttgttg acattccttt
    57121 acagctttga ctgcactttc ttttggcgga tcgatcaatc cggcgatccc tgcaaaagtt
    57181 aattgggtta aagtctcatg cgtgagttcc tgtttcgaat ctactttttt ataagcaaat
    57241 cctaaaacac gctgaccttt tctcgcaaat tctgcagctg tctgactcca tgcttcttgt
    57301 tctgcagaag aaagagttga tttagcaaat aatacttctg atgcaccttt gacgtaaatc
    57361 accgctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga actgaacgga
    57421 atctttgctt ctattttttt caaaggcaga ttggctgttt ctgtgtgtcg aatcagcgca
    57481 acttctgtcg gattaccact taaattttct gttttctgtt cttcttttgt ttttaattcc
    57541 tgacagtttt tcataatgga caacacgcag gcttcttctt ttgcagctac atccacaact
    57601 gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac agatcactgt catagaacct
    57661 aatgtttcta ccgaaggaag tcctttgatg atcgcctttt cttttgccat ttcatgtact
    57721 ccaacggata ggatcatggt caaaacagct ggcaatcctt cgggtaccat tgacaccacc
    57781 aaagcaatca tagccgaaag aaggatatgc caatccatac catatcgaaa tgtcgtaaaa
    57841 ataactaaga ataggctcaa acaaagaatg ccttgaaaaa tttgtttgtt taatcggttg
    57901 attttcttaa tcagaggcgt cgtttgttgt tcgacctatt gcaatgcttg attgatttta
    57961 ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc agattgtaca
    58021 agcgttcctg aaaaagccat atttttctgg tcgccgatcg attcttcttc tgtcaaaacg
    58081 gcatgacttt tttcagatgc ttctgattct cccgtcaaaa tcgattcttc cactaataaa
    58141 tcatgtgctt cgattaatcg gatatctgct ggaacgacat ctccggcacg taagacaatg
    58201 acatctcctt ttaccagttc tttcgcaggg attttttctt gcatcccgtt tgatagtact
    58261 atcgcttctt gtcccatcag ctgcttcaaa ccattcaatg attcttccgc ttttctttct
    58321 tgccagtaac taacgagtcc attgacgatc actacgaata aaataatact tccttcaata
    58381 tattcactag aagcaaattt caaaaaagag gcaatgatca acacgatcat tagcagatca
    58441 gtaaagtgct ttgctatttt tttccatcgt tttgtctgct gccttgcttt tatttcatta
    58501 tatccttgat cagtcaggcg tttctgtcgt tcttcttgcg acaagccttc tgttgatgtt
    58561 tgatagtttt tcaatacatc ttctaccgtc aatctgtacc agttcatcct atcccctcct
    58621 gtttaattga tactgttatt atacgcaggg aagttttttc aggcgtatcg attatttcag
    58681 gggggtctga aataattcgt tttgcagaat gtatctgggc ctaactgata tttctattgc
    58741 ttattctttt atatacaaaa tttaagaata caaaccagat aaccaataaa aaaggaataa
    58801 agcgtgtcgt gctttcgaat aacaagctga aagcaattga agtaaaaaac accaacaaag
    58861 caagatcttt atatctttgt gtagtgttcc gtttcttttc tttaataaat tgaacatgtg
    58921 tacacaagat cgaagaccac acaaatagaa aacaactcgt tgtcatgcct gccaaaagct
    58981 gaaaaaacaa tcgactatca gctacaaaaa aatgtagaca aagaccggca aaaaataaca
    59041 ttcctgtaat taaaataccg tgtaaaggta cgcctttctt agaaagcgca caaaaccaat
    59101 ggggcaaact tttctctttt gctgtttgat ataaaatcct gctagtactg tagactgcac
    59161 tatttgctga ggacaaagaa gcaaccaaaa tcattagatt gacaattgct gctccgcctt
    59221 ggcaatggat catttgcagc atagtcacta aaggactttc ttttaaatct aaacaatgcc
    59281 atggaacaac aaataaaatc gacaaaatcg ttcctatata gaaaaagaga atacgccaag
    59341 gcagacagtt gattgctttt ttcaaattct tttgcggatc acttgtctcg ccaacggtta
    59401 acccgatcat ttcaattcca gcaaaactga ataaaaccat aggaaatgag actaccattc
    59461 cagcaaagcc aaacggtagt aattcatcga tctgtccaaa attttctttg attccgatag
    59521 actgggtatg aaaatctgag acactcaatc ctatacccaa taagataaaa aggaaaatcc
    59581 ccaggatctt gatgattgag aaaaaagatt ccaattcacc aaaccacttg actgcaacaa
    59641 ggttgactgc cattaagatc attagtgcgc tacctccgct tatcaaagct ggtacatgta
    59701 caaaccagaa atggatatac aatcctattg ctgctaattc cgcaagtcca gctccaagcc
    59761 agcaaagcca ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa taatagtcaa
    59821 cgaaatcact gatagagtta aatgtactat ttgctaaaaa taaatcaccc atggcccgca
    59881 tcaaactata aatagccaaa ccgcatatca aataaacgag aataacagct ggccctgctg
    59941 atgcaattgc ctttgctgaa cctaaaaata atcctgttcc aattgcgcca cctaatgcga
    60001 ttaactggat atttcgatta gttagagtcc gtttcattgc cggtttttcc atcaagttca
    60061 cctttttctt tttcaagcag catggcgact tcctgttccc aagaaggttt ctttattttt
    60121 tcaatacaga gtggtactac aaacaaaatg acactgatgg ctgtgactgc aattagattg
    60181 atccatgttc cagctttcat cgtcagtccg acagaaaatc cgattgccgc aaataaaaca
    60241 ataacgacag tccaaactcc aagattgctc tttttctctt tttttccaat tctaaatggt
    60301 cgattcaact ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat gatgacatag
    60361 acgatacagt agataacagt agttgaattt acgagcatca aaaatgcttg attgacgcca
    60421 ggaatcagca aataaaaaag tgcaaagatt gagataatga cagactgagt caaaataacg
    60481 gatttggata cgtcaaattg attcgtcttc caaaaattga attttggtgg atacgcacct
    60541 tttctagcac tttctgtcac tgtctttgct ggtcctgagg cccaagcaga taattggact
    60601 gctacaccaa tgaagacaag taaactaaac agattcacaa taagatgtgg caaaccaaga
    60661 atttggcaat aaatcgaaat agattgcgcg atattattca attccatttg tccttttggt
    60721 acgacatttg ctacaactaa agcattcaaa atattgacga aaaatataaa aattaacgcg
    60781 gcaaatactc cgcgaatata cgttttaact ggatttttca atcgagtgat atacacagag
    60841 gacatttcga taccagtaaa aataaacatg atcggcgcaa aataaacaaa tgaggatgca
    60901 gtcgtagtat ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc ggttggtacg
    60961 atcccaactt tgatccacgc agcaaaccct aataagagca tcatcgtcaa aggcacgtac
    61021 aatcctaacc aaacaccgac ttttccgccg atttttgcca tatcaaattt caaattcaaa
    61081 aaagtgatga tccaataaac gactaaaata acaactaatg taaatttgct gttcaaacca
    61141 agcggcacat tatcgataat attaccaaac aacggagcaa gtgctgaagc gaccatcacc
    61201 atcccaggaa acatttggac ccataacagc catgagacaa cgaaccccca tttttttcct
    61261 agagcattcg atacccaaag ttctggacca ccttttttgg gaaacattcc ggcaaattca
    61321 ccagagatca atgagattgg aaaagcgtac aataatactg caaccagcat gtaaaaaatc
    61381 atcgtccagc tcgttgctgc tacatctgga atatttcgta cacttgctac aagggcgcag
    61441 gtcattccga taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc tttcatcttt
    61501 attccttctt tcaatagcga catatgtttt tgttttcttt tatttttgtt caagaacaga
    61561 aatgacaagg tttcatcgat tcagtacaaa acccagtatc accgaaacat ccattgaacg
    61621 atcttagtga actgggtttg atacatgaaa cgaacgatcc aacttataga acgttttata
    61681 aaaaagaata tcttggcgag gaatatatga aaaacagggg gaaatacaat tgttttgatg
    61741 aagcaaataa ttgtctgttc tccatatgtc tcttgctatt ctttcacaca aaaatcagtt
    61801 ctattggatg atttcgttta gtttcttttc cattgctttg gcgatttctt ttgtataaaa
    61861 attgaaaatt cgatcatcat tcaaataagg agtcatgata gccgcacgta gcagagtgat
    61921 tttctgttct tttttccatt cttctttcgt tagacccatt ctttcaacaa aacgaatcgg
    61981 agaatcaccg taatcttctt gtgtaaatgt agtatgagaa gtaataaacc gttcaccata
    62041 aacatcgcca tccatataag aagaccgatc gaacattttt tcatttaatt cattgatagc
    62101 gttcaaatct gtacagcctt gttctttgaa tacccaattg accatattaa aatcaggatg
    62161 attcaatgga tagacttcga ttgtttttcc tttaacagta aaagtcatat gatcgagaaa
    62221 ttctctgaat ctttgagctg cctcgattga agccccaatc aattgaccgt atccggtcac
    62281 attcaatggc aatacacgat gtgttgtcca gactgcggcg gcggttgctc cagcttttga
    62341 tccttccaaa atataagctc ctaacatatc tggagccttt actgattttt caaacacata
    62401 tggcgcgaaa taagaaatga tatttctcat atttttatgt ttgattacga ttcctccagc
    62461 agcataaggg acatagccca tcttatgagg atctatcgtg acagaatccg cttctgaaat
    62521 agctctgaac ccttcatata cctctttgtc aatcgttacg cagtggtgga aaacatgatg
    62581 ttcttcaaag aattcagcta aggaagcata cggcacaaat tctcctgctt cgtttagaaa
    62641 cagtgaacgc gcatagccgc catatgcggc atcaacatgt aaatagaaat agataccttc
    62701 atcttttaac ctttctctta attggacgat tttatctaca ctgtctactt gaccttcttc
    62761 tgtcgttcct acgacagcaa ccacacctag aattgggatt ttttgttcag ctaattcacg
    62821 aattgttttt tccaaagcat taatatccat tcgataatcc tcttgaactg ggatagcaac
    62881 catctgatct aagccgactc cgcaaatatc taacgccttc atccaagaat agtgttttgt
    62941 ttgagggacc agccatttcc ctaatctttg gatatttttt ccgctacggg aagaagctgc
    63001 tttgacctca tccatttctt cgtccgtaaa gctttcggtc atttccaaaa tttcttcaac
    63061 agataaattc aaaagttccc actcagacat ttttttcact ttttcaggca gcacttcctt
    63121 gacagctaaa ggaatcgatt tgatgcagcg ggcgtaccat aatccttcaa gattagcaat
    63181 agaaccatct gctgcaatgt gtccccatcc gtctgtcata ttgaataatg aagcaaaatc
    63241 ctgtcctact tctgcttcca tttgcgaagt tgccatagaa gattccaatg caacattatt
    63301 tgggttccac agcatcgcat agttgtaagc taataaagca ggcatcaatg tttcagcatt
    63361 catttgtccc cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc gtaatcgctg
    63421 actgacttca tccaagacct ctatcatatg gtcgcgggtg gctagataat ctggagtgag
    63481 cttatctcct tcgctaattg caggcatatc tgaaggaata taattttctc tccatccaag
    63541 atgttcatct accatcttgt tcaacaacat tttataaact ggtccatttt cagctttgtc
    63601 gcctataaag acggccttga tatccatatc tttcattttt cttccctcca tattttgttg
    63661 gtcaatgtct cgcagataca tgtgtttatc tgtaacacct cttcactata gcgccaaaaa
    63721 tgagaaattt tttttataac atcaaatcca cgtagatttt tacaaacaaa aataaataaa
    63781 gtcggatttt tctgataatt aaacgcaaaa aaacgaacat tttttcaata aatcagtgtt
    63841 ctaaaataga taacctaaaa aaagcgaaca aacaaata
//
LOCUS       DAZKCZ010000003        59940 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000003 DAZKCZ010000000
VERSION     DAZKCZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.003"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..514)
                     /locus_tag="ACG8KN_000118"
     CDS             complement(137..514)
                     /locus_tag="ACG8KN_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187210.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            770..1498
                     /gene="purC"
                     /locus_tag="ACG8KN_000119"
     CDS             770..1498
                     /gene="purC"
                     /locus_tag="ACG8KN_000119"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9187211.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1498..1752
                     /gene="purS"
                     /locus_tag="ACG8KN_000120"
     CDS             1498..1752
                     /gene="purS"
                     /locus_tag="ACG8KN_000120"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFP9187212.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1754..2425
                     /gene="purQ"
                     /locus_tag="ACG8KN_000121"
     CDS             1754..2425
                     /gene="purQ"
                     /locus_tag="ACG8KN_000121"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFP9187213.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2426..4648
                     /gene="purL"
                     /locus_tag="ACG8KN_000122"
     CDS             2426..4648
                     /gene="purL"
                     /locus_tag="ACG8KN_000122"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFP9187214.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4633..6072
                     /gene="purF"
                     /locus_tag="ACG8KN_000123"
     CDS             4633..6072
                     /gene="purF"
                     /locus_tag="ACG8KN_000123"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9187215.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6095..7147
                     /gene="purM"
                     /locus_tag="ACG8KN_000124"
     CDS             6095..7147
                     /gene="purM"
                     /locus_tag="ACG8KN_000124"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9187216.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7144..7722
                     /gene="purN"
                     /locus_tag="ACG8KN_000125"
     CDS             7144..7722
                     /gene="purN"
                     /locus_tag="ACG8KN_000125"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9187217.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7719..9254
                     /gene="purH"
                     /locus_tag="ACG8KN_000126"
     CDS             7719..9254
                     /gene="purH"
                     /locus_tag="ACG8KN_000126"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9187218.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9270..10544
                     /gene="purD"
                     /locus_tag="ACG8KN_000127"
     CDS             9270..10544
                     /gene="purD"
                     /locus_tag="ACG8KN_000127"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9187219.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10666..11580
                     /locus_tag="ACG8KN_000128"
     CDS             10666..11580
                     /locus_tag="ACG8KN_000128"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP9187220.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11713..13278)
                     /locus_tag="ACG8KN_000129"
     CDS             complement(11713..13278)
                     /locus_tag="ACG8KN_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9187221.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSYFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13271..14722)
                     /locus_tag="ACG8KN_000130"
     CDS             complement(13271..14722)
                     /locus_tag="ACG8KN_000130"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9187222.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14736..15752)
                     /locus_tag="ACG8KN_000131"
     CDS             complement(14736..15752)
                     /locus_tag="ACG8KN_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9187223.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15972..17060
                     /gene="chvE"
                     /locus_tag="ACG8KN_000132"
     CDS             15972..17060
                     /gene="chvE"
                     /locus_tag="ACG8KN_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9187224.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17114..18640
                     /gene="mmsA"
                     /locus_tag="ACG8KN_000133"
     CDS             17114..18640
                     /gene="mmsA"
                     /locus_tag="ACG8KN_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9187225.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18642..19847
                     /gene="mmsB"
                     /locus_tag="ACG8KN_000134"
     CDS             18642..19847
                     /gene="mmsB"
                     /locus_tag="ACG8KN_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFP9187226.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20104..21081
                     /gene="guaC"
                     /locus_tag="ACG8KN_000135"
     CDS             20104..21081
                     /gene="guaC"
                     /locus_tag="ACG8KN_000135"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9187227.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21345..22769
                     /locus_tag="ACG8KN_000136"
     CDS             21345..22769
                     /locus_tag="ACG8KN_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9187228.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22811..23068)
                     /locus_tag="ACG8KN_000137"
     CDS             complement(22811..23068)
                     /locus_tag="ACG8KN_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187229.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23230..24339
                     /gene="pdhA"
                     /locus_tag="ACG8KN_000138"
     CDS             23230..24339
                     /gene="pdhA"
                     /locus_tag="ACG8KN_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFP9187230.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24342..25319
                     /locus_tag="ACG8KN_000139"
     CDS             24342..25319
                     /locus_tag="ACG8KN_000139"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFP9187231.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25344..26987
                     /locus_tag="ACG8KN_000140"
     CDS             25344..26987
                     /locus_tag="ACG8KN_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9187232.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26995..28401
                     /gene="lpdA"
                     /locus_tag="ACG8KN_000141"
     CDS             26995..28401
                     /gene="lpdA"
                     /locus_tag="ACG8KN_000141"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9187233.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28563..28838
                     /locus_tag="ACG8KN_000142"
     CDS             28563..28838
                     /locus_tag="ACG8KN_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFP9187234.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28844..29611
                     /locus_tag="ACG8KN_000143"
     CDS             28844..29611
                     /locus_tag="ACG8KN_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFP9187235.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29779..31608
                     /gene="typA"
                     /locus_tag="ACG8KN_000144"
     CDS             29779..31608
                     /gene="typA"
                     /locus_tag="ACG8KN_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFP9187236.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31869..33641
                     /gene="recQ"
                     /locus_tag="ACG8KN_000145"
     CDS             31869..33641
                     /gene="recQ"
                     /locus_tag="ACG8KN_000145"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFP9187237.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33755..34057
                     /locus_tag="ACG8KN_000146"
     CDS             33755..34057
                     /locus_tag="ACG8KN_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFP9187238.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34060..35223
                     /locus_tag="ACG8KN_000147"
     CDS             34060..35223
                     /locus_tag="ACG8KN_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9187239.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35249..38677
                     /locus_tag="ACG8KN_000148"
     CDS             35249..38677
                     /locus_tag="ACG8KN_000148"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFP9187240.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38698..39822
                     /locus_tag="ACG8KN_000149"
     CDS             38698..39822
                     /locus_tag="ACG8KN_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFP9187241.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39803..40249
                     /locus_tag="ACG8KN_000150"
     CDS             39803..40249
                     /locus_tag="ACG8KN_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9187242.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40233..40571
                     /locus_tag="ACG8KN_000151"
     CDS             40233..40571
                     /locus_tag="ACG8KN_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFP9187243.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40690..41250
                     /gene="rsmD"
                     /locus_tag="ACG8KN_000152"
     CDS             40690..41250
                     /gene="rsmD"
                     /locus_tag="ACG8KN_000152"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFP9187244.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41247..41738
                     /gene="coaD"
                     /locus_tag="ACG8KN_000153"
     CDS             41247..41738
                     /gene="coaD"
                     /locus_tag="ACG8KN_000153"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9187245.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41728..42771
                     /locus_tag="ACG8KN_000154"
     CDS             41728..42771
                     /locus_tag="ACG8KN_000154"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFP9187246.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42930..43658
                     /locus_tag="ACG8KN_000155"
     CDS             42930..43658
                     /locus_tag="ACG8KN_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9187247.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43774..44019
                     /locus_tag="ACG8KN_000156"
     CDS             43774..44019
                     /locus_tag="ACG8KN_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9187248.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44016..44234
                     /locus_tag="ACG8KN_000157"
     CDS             44016..44234
                     /locus_tag="ACG8KN_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9187249.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44370..44909
                     /locus_tag="ACG8KN_000158"
     CDS             44370..44909
                     /locus_tag="ACG8KN_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFP9187250.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44902..47175
                     /locus_tag="ACG8KN_000159"
     CDS             44902..47175
                     /locus_tag="ACG8KN_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFP9187251.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47213..48259
                     /gene="holA"
                     /locus_tag="ACG8KN_000160"
     CDS             47213..48259
                     /gene="holA"
                     /locus_tag="ACG8KN_000160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9187252.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFASYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48348..48602)
                     /gene="rpsT"
                     /locus_tag="ACG8KN_000161"
     CDS             complement(48348..48602)
                     /gene="rpsT"
                     /locus_tag="ACG8KN_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9187253.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(49002..49271)
                     /locus_tag="ACG8KN_000162"
     CDS             complement(49002..49271)
                     /locus_tag="ACG8KN_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187254.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49533..49661)
                     /locus_tag="ACG8KN_000163"
     CDS             complement(49533..49661)
                     /locus_tag="ACG8KN_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187255.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49741..50196)
                     /locus_tag="ACG8KN_000164"
     CDS             complement(49741..50196)
                     /locus_tag="ACG8KN_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9187256.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50334..52262)
                     /locus_tag="ACG8KN_000165"
     CDS             complement(50334..52262)
                     /locus_tag="ACG8KN_000165"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9187257.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52459..53292)
                     /locus_tag="ACG8KN_000166"
     CDS             complement(52459..53292)
                     /locus_tag="ACG8KN_000166"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9187258.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53484..54188)
                     /locus_tag="ACG8KN_000167"
     CDS             complement(53484..54188)
                     /locus_tag="ACG8KN_000167"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9187259.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54211..54837)
                     /locus_tag="ACG8KN_000168"
     CDS             complement(54211..54837)
                     /locus_tag="ACG8KN_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9187260.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55163..57538
                     /locus_tag="ACG8KN_000169"
     CDS             55163..57538
                     /locus_tag="ACG8KN_000169"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9187261.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57562..58053)
                     /locus_tag="ACG8KN_000170"
     CDS             complement(57562..58053)
                     /locus_tag="ACG8KN_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFP9187262.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58240..59160
                     /locus_tag="ACG8KN_000171"
     CDS             58240..59160
                     /locus_tag="ACG8KN_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9187263.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59314..59658
                     /locus_tag="ACG8KN_000172"
     CDS             59314..59658
                     /locus_tag="ACG8KN_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFP9187264.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59848..>59940)
                     /locus_tag="ACG8KN_000173"
     CDS             complement(59848..>59940)
                     /locus_tag="ACG8KN_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9187265.1"
                     /translation="NLGSLKAQQKNGAAIYREDEKEYYTKIKLM"
BASE COUNT        19671 a        10363 c        12872 g        17034 t
ORIGIN      
        1 ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta ttatattttc
       61 ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc ctctttacat
      121 acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa tgctggttat
      181 ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca ttgaataaga
      241 tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg attgaaagtt
      301 cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca cagcgcttgg
      361 ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat aaataaacgt
      421 ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt aattctgata
      481 aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc gaacccatta
      541 tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa cgcttgttga
      601 tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa tgccttaaat
      661 gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa
      721 caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca tggagaagaa
      781 agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg agaacttgat
      841 ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag accagataag
      901 tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc tgactcaaag
      961 aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt
     1021 acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt tctcaaaacg
     1081 tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct actataagga
     1141 tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac aacttgcaaa
     1201 gccggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa ttttgaccga
     1261 attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg gccggtcgaa
     1321 tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat gggatgaacg
     1381 tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt tgatcccagt
     1441 ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc gagtatgatg
     1501 tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca aggggaggca
     1561 gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg aataggaaaa
     1621 tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt
     1681 tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg
     1741 gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga
     1801 tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac ggcatgatga
     1861 gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct atggtgacta
     1921 tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga ttcgttttgc
     1981 tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga cagaagcagg
     2041 acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca aaactgtacc
     2101 attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg aaataatcta
     2161 tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac ttcaagaact
     2221 aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca gcgtggaaaa
     2281 cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc atccagaacg
     2341 tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt ccattttaaa
     2401 aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag ctgaagagat
     2461 caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc gaatgatcga
     2521 agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt ctgttatgtg
     2581 gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc ctactagtgg
     2641 acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg gtgatggact
     2701 agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc cttatgaagg
     2761 agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg ctcgtccaat
     2821 tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga aatatttact
     2881 agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac cgactgtagg
     2941 cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg caatgtgcgt
     3001 aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg gtaattcgat
     3061 catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt ttgcctcaga
     3121 agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg atccttttat
     3181 ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg attctctaat
     3241 aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa tggcttcaaa
     3301 agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa ctgggatgac
     3361 accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg ttaaatcagg
     3421 agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag tcactatcgg
     3481 taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag tagcaaatct
     3541 gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga aagaaccagc
     3601 tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag aacctggaca
     3661 agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa tctacgaaac
     3721 ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat
     3781 gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg cgcgctatct
     3841 gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta gaaatattat
     3901 tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt ctcctgataa
     3961 accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg cgtgtgaaac
     4021 gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg atggtcaagc
     4081 aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata aacatatcac
     4141 tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga cgtttgcaga
     4201 ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa
     4261 ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca tccaaaacgg
     4321 attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac tggcagaatc
     4381 agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg ctcaattatt
     4441 tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga cacgattcga
     4501 agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag cgataatcga
     4561 aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa agtgctggga
     4621 ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt
     4681 gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag tcttcagcat
     4741 cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga
     4801 gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca gttaacagga
     4861 gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt ggacaatatt
     4921 cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa tggtaacttg
     4981 atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt ccattctaac
     5041 tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga ttttttaaat
     5101 aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt aatgacagaa
     5161 acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat cggacaaatg
     5221 aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat tggggcacgt
     5281 tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg aatccagatt
     5341 gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat ctattttgcc
     5401 agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa tatgggacgg
     5461 aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc taattcttct
     5521 ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat cggattagta
     5581 aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag agagcaaggt
     5641 gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt gattatggtc
     5701 gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt gaaagaagca
     5761 ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc atgtttttac
     5821 gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat tgatgaaatc
     5881 aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct gatcgaatcg
     5941 atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt caacggagat
     6001 tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa agaaaaagta
     6061 tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa atgcatacgc
     6121 aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca agaagcatgt
     6181 caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg gttgttttga
     6241 tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg gcgtggggac
     6301 gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg actgcgtagc
     6361 gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct tagactactt
     6421 agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg
     6481 atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc ctggtatgta
     6541 ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa aatctcaatt
     6601 attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat ctagcgggat
     6661 tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc agttttcttt
     6721 atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa
     6781 aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag gagctgcaca
     6841 tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat ctatggctgc
     6901 acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac aaaaatatgg
     6961 tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta tggtcctggc
     7021 agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt
     7081 ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta taggagaaga
     7141 acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc gctagccgat
     7201 tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga tcagcctgaa
     7261 gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt ttcacctaaa
     7321 gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag
     7381 atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt gctgaaaaat
     7441 tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc tggtttgcat
     7501 ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat tcattatatc
     7561 gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat tgaacaggag
     7621 gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat ctatccagaa
     7681 gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca ttgatcagtg
     7741 tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa ggagtagaaa
     7801 tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa acgctttcga
     7861 tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca cttcatccta
     7921 aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag gcaatggaaa
     7981 cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc aaagaaacga
     8041 ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc ggtggtccta
     8101 gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt gatccatctg
     8161 actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt tctttcagac
     8221 aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg atcgctcaat
     8281 acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa
     8341 aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat cagtccgctc
     8401 ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa ctttcttata
     8461 ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac gagccaacgg
     8521 tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg attttagaag
     8581 cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc atcgttttga
     8641 atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta gaaatcatca
     8701 ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa aacatccgat
     8761 tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg gtctcagtct
     8821 taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa gagtggaaag
     8881 tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt gcttggagag
     8941 ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa
     9001 tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag
     9061 agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat gattcggttg
     9121 aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc atcaaagacc
     9181 aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact gatgttcggc
     9241 attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc
     9301 gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg gtgtattgtg
     9361 cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc agtgagcgag
     9421 atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt atcgttggtc
     9481 ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga ttgaaaatct
     9541 tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct aaacaattaa
     9601 tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac gaaaaagcag
     9661 cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg
     9721 ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca gaacaaatgt
     9781 tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag tttttggcag
     9841 gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt
     9901 cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc ggaatgggtg
     9961 cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg gaagaagttt
    10021 taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt gttttatatg
    10081 cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg
    10141 atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg
    10201 atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac aatgtaggcg
    10261 ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt ctaccagatt
    10321 ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac ggcaaactag
    10381 ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc aaagaagcac
    10441 agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt tatcgtacag
    10501 atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt
    10561 tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct tttcataaag
    10621 agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa aaaaattcaa
    10681 ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg tatcaacaat
    10741 gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga tttttttgac
    10801 catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa
    10861 acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt tccaggtgtg
    10921 atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt gacccgtctg
    10981 aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt agaaccagag
    11041 gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc
    11101 agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca accattagta
    11161 gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg gattcatgtc
    11221 aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta ttcccgattg
    11281 catgatatga cgatccaagc atggtcacca taccagtatg gattttttga aggtgtattt
    11341 atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc agctaaatat
    11401 caaacgacac caactggctt ggcttctgct tggattttac gccatccagc caatatgcaa
    11461 gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt
    11521 aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat tttaccttaa
    11581 agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa cttgaaagct
    11641 gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt atcattgtac
    11701 ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat attcttttgg
    11761 agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat tataaccaat
    11821 ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt ttgctaattc
    11881 gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt
    11941 actgaaataa gagggactta atgcaacttg ttgagcgacc caagttaagg acatatctgg
    12001 atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct gatatttgtt
    12061 ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg
    12121 gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc aatgaaccat
    12181 ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag tcaagacaaa
    12241 taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta
    12301 cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa agtaaaagac
    12361 agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa tttgttcttt
    12421 aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag gtaattcgct
    12481 gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc ccttttctaa
    12541 ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt cttcaagaga
    12601 atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca cttgttgatt
    12661 ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa agtctgtact
    12721 gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc gattgaaaga
    12781 ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa
    12841 atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa
    12901 atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt taatcggttt
    12961 aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga aatcgtcgaa
    13021 tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt
    13081 caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct gtaagtcgat
    13141 aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga ttggataata
    13201 agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt cgtcttcgat
    13261 gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga gggatcatca
    13321 gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt tgatcatttc
    13381 cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg
    13441 aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca gggatgccaa
    13501 tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgtg gtaatacgaa
    13561 taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca atcaatggct
    13621 gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg atcttatagt
    13681 caagcatatg gccgtatctt atttttaaga tgactagata atcattgata tggctaattt
    13741 ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa
    13801 gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg
    13861 catccaaact gttataaata aaatggggag taatctgagc ttgcaataca cgaacttcac
    13921 tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt agatcttccg
    13981 ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc agggttaaac
    14041 gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga ttgatcggtt
    14101 cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa atggtactaa
    14161 gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc gtattttttt
    14221 tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac agcagttgag
    14281 tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct tttgccaaga
    14341 tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt agcgatacat
    14401 ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga atatcattcc
    14461 taagttcttc taggatattt tcctcattga actcatctac ggttacttgg ccagaaatca
    14521 agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca tctgcttttt
    14581 ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac agtatgctca
    14641 gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca aaaatctgtt
    14701 ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt tgggatgtta
    14761 gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg taatgttttt
    14821 cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca gccgacatca
    14881 ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat catatcttct
    14941 tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt ttcttttatc
    15001 gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc gatttcggat
    15061 aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg aataaaatca
    15121 ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc atcttttttt
    15181 agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg
    15241 gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga atttgtgaag
    15301 tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg gccaatgtgg
    15361 gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat gacagggatc
    15421 cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa aggagtaaaa
    15481 atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg ccgttcctga
    15541 ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc agtgataatt
    15601 gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa accaacgact
    15661 agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta agcgattaca
    15721 acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg gatttgttag
    15781 tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc
    15841 aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa agtaaatccc
    15901 ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc aattgaaatg
    15961 ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct attttgaccg
    16021 caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt acggggtatg
    16081 taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg aacaacatgg
    16141 tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa gataaagtag
    16201 aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca cttgtgattg
    16261 cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa gcagatatca
    16321 aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat tacgcgactt
    16381 ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac ttaggattga
    16441 aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac gacaacaacg
    16501 cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac aacggtcagt
    16561 tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt
    16621 ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac caaacattgg
    16681 atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca ttaaaaggcg
    16741 tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat gcgacagttg
    16801 ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa gacacacgta
    16861 tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa gaggttccgg
    16921 tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat ttagcaaata
    16981 cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat tattataaag
    17041 aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct aagtcagaga
    17101 gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca aaaaattttc
    17161 tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt
    17221 gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc
    17281 atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat ttaaagattt
    17341 gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac taagtcctta
    17401 tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag gcgtgattga
    17461 ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt tgtctatcga
    17521 cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg aaatagctaa
    17581 agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg cattgaatga
    17641 agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag gtgtaacctc
    17701 catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt
    17761 acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag agcatattat
    17821 tcgcggaatg gtgggcagag atttagttga tcgttatcct gaaagaaaac cggatattgg
    17881 agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc acgagcgaat
    17941 cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa tagctggact
    18001 gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt atggaagcaa
    18061 gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg ttccttcagc
    18121 cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac tgaatctgct
    18181 gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc aaaaaggagt
    18241 attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga tgcgtataaa
    18301 aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt
    18361 tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc caacccgtgg
    18421 gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg cagcaaatgg
    18481 caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt gtgatcgaat
    18541 ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg cagatcagga
    18601 agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc
    18661 ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat ctttttggtc
    18721 gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg atgacggatg
    18781 gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc catatattag
    18841 tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg tcggtagggt
    18901 cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc
    18961 cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca tggcaaggtt
    19021 tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga ttgctgatgt
    19081 ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt ccagctgttt
    19141 tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc cttcacttct
    19201 tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa
    19261 gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc ttggtaaagg
    19321 cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct tatcaagggt
    19381 atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt ttgacaaata
    19441 aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg gctgaactat
    19501 cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga gtcatggcag
    19561 ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa gcaggtacat
    19621 cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact tccggcggca
    19681 ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg aacaatggca
    19741 tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg atcttgcttt
    19801 tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag aacgccggtt
    19861 attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct aaaagcgaac
    19921 attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca ttcgtataaa
    19981 ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg gcgctaaaaa
    20041 aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca ggaggataat
    20101 gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg tatcgtaaat
    20161 agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa aatgccagta
    20221 gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt agcggaaaat
    20281 ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa
    20341 atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga ggaatattcc
    20401 tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat cgacatcgcg
    20461 catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata tttgcctgaa
    20521 acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg
    20581 ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac aaaaatcaag
    20641 acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc gaaagcagct
    20701 cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc aaaatctgtc
    20761 cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga ggaatctcct
    20821 ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc tgcttcagaa
    20881 ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca acacaaagga
    20941 tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat ttcttatgct
    21001 ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt
    21061 tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga aaaagttgtc
    21121 ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt tgtttggctg
    21181 gaattattat cgcagataca agcttttgaa catcattatt cagaataagg gactgtgata
    21241 agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt
    21301 cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg gaaaaagtag
    21361 agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa
    21421 acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg caactttttg
    21481 gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt ggggtactaa
    21541 cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa ttcgctgttc
    21601 caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac tggtatgagc
    21661 aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta tacgctagcg
    21721 agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt gaagaaatct
    21781 tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac aaaatcaaac
    21841 ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt gtgactggtg
    21901 gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga gaggggatag
    21961 ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca ggaacgcgtt
    22021 tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc
    22081 ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct acaggaaaag
    22141 tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat cggcttccaa
    22201 ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa gtccttccta
    22261 tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg gttttaggaa
    22321 aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa ctagctcaag
    22381 cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg gctaatgtat
    22441 ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc
    22501 gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca attttaggtg
    22561 gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta gcagaaaaag
    22621 tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat ataaatggaa
    22681 tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat tcaaagatga
    22741 aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta aagaatataa
    22801 cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata cgatataaag
    22861 aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt
    22921 tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg taagtttgat
    22981 tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg attttattca
    23041 ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat ttgtgctttt
    23101 agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa atagtgatac
    23161 agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg agagaggaat
    23221 gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct agatgaagtc
    23281 aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt taacaaagat
    23341 ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat ggtatggtct
    23401 cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg cttttttgca
    23461 ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga taaagaagat
    23521 gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt acctttatct
    23581 caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga agaattgaaa
    23641 gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg tgttgcatta
    23701 ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga tggcggttca
    23761 tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc aaatgccgta
    23821 ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca aactgcagca
    23881 aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt cgatggaatg
    23941 gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat cagtggaaat
    24001 ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact atctggtgac
    24061 gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa agatccattg
    24121 atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa
    24181 gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga caaagtacca
    24241 aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca atcaatcaaa
    24301 gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa aaaacaatga
    24361 tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat gtcgttgttt
    24421 ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga ttgcaggaaa
    24481 agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc gcaggattga
    24541 gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc tttggtttta
    24601 ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat cgaatgagcg
    24661 gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt catacaccag
    24721 aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt cgagtagtga
    24781 ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga agtaacgatc
    24841 cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa gtgccggatg
    24901 aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca gatgtatcta
    24961 ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag
    25021 aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat gtagaaacga
    25081 tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca caacgacaag
    25141 cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt tcattagaag
    25201 ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa gcagaaaatg
    25261 tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg gaattttaag
    25321 aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa attgccggat atcggtgaag
    25381 gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg atcaatgaag
    25441 atgatacatt actagaagtt caaaatgaca aatcagtaga agaaatcccg tcacctgtta
    25501 caggaacggt taaaaatgtg attgttccag aaggtaccgt agcaaatgtt ggggatgtat
    25561 tagtagaaat cgatgcacca ggacacgaag acaatgaagg ggacagtggt gtagccgcag
    25621 aatcccaaac tccagctaaa ccggctgctg aacccactgt tgatacagaa tcagctggat
    25681 cgtcaagcga aggagtcttc caatttaagt taccggatat cggtgaagga atcgcagaag
    25741 gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat gatacattac
    25801 tagaagttca aaatgacaaa tcagtagaag aaattccgtc acctgtcaca gggacagtta
    25861 aaaacgtgat tgttccagaa ggcactgtag caaatgttgg ggatgtatta gtagaaatcg
    25921 atgctcctgg acacaatagt gcaccgtcaa ctagtgcgcc atcagctgaa gcaccaaaag
    25981 aaaaagtgga aacatcaggt tcagcaagtg tcgttgaagc tgccgatcca aataaacgtg
    26041 tattggctat gccatctgtt cgtcaatttg cccgtgaaaa agatgtagat atctcacaag
    26101 taactgcaac tggaaaaggt ggcagagtaa caaaagagga catcgaaaac ttcttggcag
    26161 gtggtccaag cagtgcacca gcgaaatcag aagcgccaga agcggcagca ccgaaagaag
    26221 cagctcctgc agcagaaagc aaaccagctg caccagcaaa accattcaag tccaacttag
    26281 gagacttgga agaacgtgta gcaatgacgc caacacgtaa agctatcgct aaagcaatgg
    26341 tcaacagtaa gcatacagct ccacacgtga cattgcatga tgaagtagaa gtgtctaaac
    26401 tatgggataa tcggaaacga tttaaagaag ttgctgctgc aaatggtacg aaacttactt
    26461 tcttgccata tgtcgtgaaa gctttaacag cgactgttaa gaaataccca gtattgaatg
    26521 catctatcga cgatgcaaac caagaaatcg tttataaaca ttactacaat atcggtattg
    26581 caactgatac ggatcacgga ctatatgtac caaatgtgaa agatgcagac cgcaaaggaa
    26641 tgtttgctat tgcagacgaa atcaatgaga aagcaaaatt agctcatgac ggcaaactat
    26701 ctgcagaaga tatgcgcaat ggaacaatta cgatcagcaa tatcggatct gtaggtggcg
    26761 gttggtttac accagtcatc aactatccag aagtagctat tttaggtgta ggaacgattg
    26821 cacaacaacc aatcgtaaat gctgaaggcg aaatcgttgt cggtcgtgtg atgaaactgt
    26881 cattaagttt cgatcaccgt atcgtagatg gagcaactgc gcaacaagca atgaataata
    26941 tcaaacgtct tttagctgat ccagagctat taatgatgga aggatgataa acaaatggta
    27001 gttggagatt ttgctgtaga attagataca gttgtaattg gtgcgggacc tggtggctat
    27061 gtagctgcca tccgcgctgc tgaaatggga caaaaagtag caatcattga aagagagtat
    27121 attggcggtg tttgtttgaa tgtcggctgt attccttcaa aagcactgat agctgcagga
    27181 caccattatc aagagtcact agattctgaa atgtttggag taactagtga aaacgtcaaa
    27241 ctagattttg caaaaacaca agaatggaaa gaaaacaaag ttgtccatac gttgacttct
    27301 ggtgttggtt tcctattaaa aaaacataag gttgaaacaa tcgaaggaga agcgttcttc
    27361 gtagatgatc acacactacg cgtcattcat ccagattcag ctcaaactta ttcgttcaat
    27421 aacgcaatca tcgcaacagg ttcccgtcca atcgagattc caggatttaa attcggagga
    27481 cgtgtgttag attctactgg cggattagca ttgaaagaag tacctaagaa atttgtgatc
    27541 atcggtggcg gtgtgattgg tgccgaattg ggcggagcgt acgcaaactt aggagcagaa
    27601 gtaacgattc ttgaaggctc accacaaatt ttaccaactt acgaaaaaga tctagtaaaa
    27661 ttagtagaag atgatttcaa gaaaaaaggc gtaacagtcg taacgaatgc aatggcgaaa
    27721 gaagcagtcg ataacggtga cagcgtgaca gtgaaatatg cagtggacgg gaaagaagaa
    27781 tccgttactg ctgattatgt gatggtcact gtaggtcgtc gtccgaatac agatgatatg
    27841 ggcttagaac aagcgggtgt cgaagtagga gaacgaggat tgatcacggt ggataaacaa
    27901 ggacgtacga acgtacctaa catctatgcc atcggtgata tcgttccagg ggcggcatta
    27961 gcacacaaag ccagttatga agcaaaaatc gctgctgaag caatctctgg caaaaaagta
    28021 gctgtagatt ataaagcaat gccggcagta gccttcactg atcctgaatt ggcttctgta
    28081 ggtatgacga tcaaagaagc aaaagatgct ggtatcgaag caaaagcata taaattccca
    28141 ttttcaggga acggtcgggc attatcgctt ggtaaaacag aaggattcat ccgtctagtt
    28201 acgacgatcg aagataacgt gcttattggc gcgcaaatcg gtggagtcgg cgcaagtgat
    28261 atggtctctg aattagcttt agcaatcgaa tcaggaatga atgctgaaga tatcgccttg
    28321 acgattcatc ctcatccatc tcttggcgaa atcgtcatgg atgcttcgga attagcatta
    28381 ggtttaccaa tccatatttg atttgaataa tacaggcgaa acgcaactga aaaatcagtt
    28441 gcgttttgtt ttttagaatt atcaataata ttattatgta aacttaaaaa tatgacaact
    28501 tcttggttta ttaccacttc ctatgctaca ctgaagagga atatgaaata ggagagttga
    28561 agatgcaaga atatcagtat ccacttgatt tagattggac aacagacgaa atggtcattg
    28621 taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa agggataaac aatgaagctt
    28681 ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc cattggtgag gaaaaacgat
    28741 tgggacggga atttgaaaaa gcttccggtt attctttata tcgtacagtg cagcaagcaa
    28801 aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga taaatggaac agatgattga
    28861 tcatatcaag tcctggcttg aagaagctgg tgagaaaatc aaatcttctc aacaaacatt
    28921 aacaatctcg caaaaatcaa atcgaacgga tctagtaaca aatgtcgatc gaaacatcca
    28981 gaattttcta atcgaaaaaa tccattcctt tgatccagag gcgaaaatat tggcagaaga
    29041 agaagggtgt aatcaattat cggatctcga tggaagagtc tttatcatcg atcctattga
    29101 tggcaccttg aattttgtga ttgaagggga aaatttttgt attatgctcg ctgtttatga
    29161 atccggcgtt ggaaagcttg gtttcattta tgacgtgatg agaaatgaac ttttttgggg
    29221 tggaaaagga ataggcgtgt ataaaaatga tgagccattg cctagaccag caaataaatc
    29281 cttatcggaa ggcttacttg gtgtgaatag ttatcttttc ggtcataata aatatggaat
    29341 ccgagaaatt gcggaaagca gcatggggac acgtatgtac ggatgcgctg gtcttgaact
    29401 agtggcaatc ttgaaaggaa accacattgc ttatatttca aaccttagcc cttgggatta
    29461 tgcagcggga aatgtcttat tggaagagtt tggtatggta acaagcggtc tttttggtac
    29521 tcctttgaca tttgaaaaaa gggaatacta tttagcaggg acttcttgtg catatgcaga
    29581 aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt agaaatcatt cacaaaaatg
    29641 ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa aacattttac tatatttcat
    29701 gaaaaaagtc tgctataatt agcagtcgag taaaatttgc cctgtcataa agaaccaata
    29761 aaaaaggagt tagttacatt gaattataga aacgatatcc gtaacgtcgc aattattgcc
    29821 cacgttgacc acggaaaaac aacattagtt gatgaactat taaaacaatc agatacatta
    29881 gacgcacata cgcaattgca agaacgtgcg atggactcaa atgcgctaga aaaagaacgt
    29941 ggtatcacga ttttagccaa aaacacagct gtagactata aaggtattcg cgtaaacatc
    30001 atggatactc caggacacgc ggacttcggt ggcgaagtag aacggatcat gaaaatggtt
    30061 gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa tgcctcaaac acgttttgta
    30121 ttgaaaaaag cactggaaca acatatcaca ccaatcgttg tcgtaaacaa gattgacaaa
    30181 ccttctgctc gtcctgagca tgtcgtagat gaagttcttg aactattcat cgaattagga
    30241 gcagatgatg atcagttaga ctttcctgtg atttatgcat ctgcactaaa tggtacgtca
    30301 agcttgtcag atgatccagc agatcaagaa ccaaccatgg cacctatttt tgatacaatt
    30361 attgaaaaaa tccctgctcc tgtcgataac agcgacgaac cattacaatt ccaagtttca
    30421 ttacttgact acaacgatta tgtaggtcgt atcggaatcg gacgtgtatt tagaggaaca
    30481 atcaaagtag gagaccaagt agcattaatt aaattagacg gaacagttaa gaaattccgt
    30541 gtaacgaaat tatttggttt ctttggacta aaacgtctag agattcaaga agcaaaagct
    30601 ggagacttga ttgctgtctc aggtatggaa gatatctttg tcggtgaaac agttactcct
    30661 gtggatcatc aagatgcgtt acctatcctt cacattgacg aaccaacatt gcaaatgact
    30721 ttcttagtta ataattcacc atttgccggt cgagaaggta aattcgtaac cgctcgtaag
    30781 attgaagaac gtttgatggc agaacttcaa acagatgtat ccttacgagt tgaaccaaca
    30841 aactctccag atgcatggac agtttctggt cgtggagaat tgcatttgtc tatcttgatt
    30901 gaaaacatgc gccgtgaggg atacgaattg caagtatctc gtccagaagt tattgaaaaa
    30961 gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc aaatcgatac accagaagaa
    31021 tatatgggta gtgttatcga gtccttgagc ttacgtaaag gtgaaatgca agatatggtc
    31081 catacaggaa acggacaaat ccgtttgaca ttcttaacac ctgcccgcgg tttgattggt
    31141 tattcaactg aattcctttc aatgacacgt ggttacggaa tcatgaacca tacttttgac
    31201 caatatctgc caatgcttcc tggtcaaatt ggcggacgtc accaaggggc gcttgtttcg
    31261 atcgatacag gaaaagcaac gacttattct atcatgagta tcgaagaacg cggtacagtc
    31321 ttcgtagaac caggtacaga agtatatgaa ggaatgatta tcggggaaaa cagccgtgat
    31381 aatgatttga ctgtcaacat cacgaaagca aaacaaatga caaacgtacg gtctgctact
    31441 aaagaccaaa catctgtcat caaaaaacca aaacaattga cgttggaaga atctttggaa
    31501 ttcttgaatg atgatgaata ttgcgaagta acaccagaaa gcattcgctt gagaaaacaa
    31561 attttagaga aaaatgctcg tgaaaaagca tctaaaaaga aaaaataatc tgtaaaagga
    31621 tgaagagaga aaccagcttg ttataaaaaa tagctggttt ctttttttgt ccaactcttt
    31681 tctgtggaga agtcatcttt gtcaatggac ataaattcag aaaatacgcc gaaagacgtt
    31741 taaaagactt ttaaaaaact tatttctaga gagtttttgt ttcataataa gataatgttt
    31801 cacgaaagga gatcaacttg tccttcgaag aaaatcgaag taaaatggca agagaaagga
    31861 gcgggaagat gaacgactta ttaaaacaat actttggcta tgatgagttt agacctggtc
    31921 aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt attaggaatc atgcctaccg
    31981 gaagtgggaa atcaatctgt taccagcttc cagctttact attagatggg cttactgtgg
    32041 ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga cgcggcgaat caattaggta
    32101 tacccgccac ttttatcaat agctcgttag atggttacga aacagctaga cgcttccaag
    32161 aaatcgatag acagcagtac cgtttacttt atatcgctcc tgaacgtttt atcatgccgg
    32221 attttatcca agcaatgaag cgatggaacg tccgtatgat cgctattgac gaggcacatt
    32281 gtatctctca gtgggggcat gatttccgcc caagttattt acagatggcg aaccagctag
    32341 atcaactgcc aaatcgtcct gtaattgttg ctttgacagc tacagcgact gtgcaagtag
    32401 ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa ccatattcag actggttttg
    32461 aacgagaaaa tctacgattt caagtaatta aagatcaaaa aaaagaacaa tatctaatcg
    32521 aatatttaaa gatcaacaaa aatcaatctg gtatcattta tgcagcaacg agaaaagaag
    32581 tagaccgtct ctatcatttg cttaaaaaat tcgatttttc cgtgggtaga tatcatggtg
    32641 gtctaaatga gaatgagcga acagaaatgc aagaagcctt tttatacgat cgcttgcagc
    32701 tgattgtagc gacaaatgca tttggtatgg gtataaataa aagcaatgtc cgttttgtta
    32761 tccattatca aattcctggc tctctagaag catactatca ggaagctggt cgggcgggaa
    32821 gagatggtct ttccagtgaa gccatcctgc ttttctctcc tcaagatatc caagtccaaa
    32881 agttttttat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa tatgaaaaat
    32941 taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac atcctaaact
    33001 attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat gacagagaaa
    33061 tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt atgggtgaga
    33121 actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa aagctgcaag
    33181 ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg caaaaagaaa
    33241 cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc aatggtgagt
    33301 atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa gaatctgttt
    33361 atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat acactatttg
    33421 aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc ccgtatgtcg
    33481 tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc agattggaga
    33541 tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa gcctttttat
    33601 ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga atacgtaaaa
    33661 atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat caaatatgtt
    33721 ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga aaaaacttgc
    33781 gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa atcaaaacaa
    33841 cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac agatgtacgg
    33901 cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga acgagggcca
    33961 gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg tttaccaaga
    34021 aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa agtgaaaaaa
    34081 aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt attgggattg
    34141 atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa tccgtccgca
    34201 tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc actactttat
    34261 aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat cgcagttttg
    34321 acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc gaaaggttgg
    34381 ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat ttctatctgg
    34441 tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt tcttgggact
    34501 gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat tcttcccgat
    34561 tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc cagcggagtc
    34621 aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac ttttacgatc
    34681 tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta tatctataat
    34741 cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca tcaactagtc
    34801 aacggctact atgccatgtt caacggagga ctatttggca ggggattagg taacagtatc
    34861 cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat cgtagtggaa
    34921 gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat gattgtgcgc
    34981 attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg cattgggatc
    35041 ggcgcaatgt ttttgatcca agtatttgtc aatcttggag gaattactgg cgttattcct
    35101 ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct gatgctttcg
    35161 atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag cttaggttta
    35221 taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta acaggggtga
    35281 aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg ttggaatcta
    35341 tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat atttagtagg
    35401 acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt ctatcgcgaa
    35461 agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa acttgaattt
    35521 tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc atcatttaga
    35581 tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga ttgcttcgat
    35641 tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg cgaaacaata
    35701 cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca tgcgtgtagc
    35761 tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag caaaagcagc
    35821 ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc atatcgaagt
    35881 acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag attgttctgt
    35941 tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga atgaacagca
    36001 acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt atgtcaacgc
    36061 aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag tcaaccctcg
    36121 cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg taacaacgca
    36181 actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc agcaagaagg
    36241 aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc cgttgaataa
    36301 ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt ttggcgtacg
    36361 tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg attcattgct
    36421 agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa tggaacgttg
    36481 cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc tcaatgtcat
    36541 cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata gtacagcaac
    36601 attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat atatcggtga
    36661 aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt atgaagaacc
    36721 acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga atgtattaga
    36781 tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt tgttgttgac
    36841 ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc ggacaaagga
    36901 tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt caagtgagat
    36961 gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc cttggcaacg
    37021 gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt tcagaggttc
    37081 aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg tcaaagaagc
    37141 agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga ctccacaaat
    37201 ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag ctatttgcta
    37261 tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt ataaagacat
    37321 ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata tggctgggct
    37381 gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga cagatctgcc
    37441 aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt ctgctgcttc
    37501 aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga atactagtca
    37561 gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc cagaaatcac
    37621 gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt attacaaacc
    37681 gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa tgccaggagg
    37741 acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc gctgggatga
    37801 aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta aagtaactcc
    37861 atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc ttacagaaga
    37921 agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga ctttcttcca
    37981 aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga ttattcttaa
    38041 aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg acttcaacga
    38101 agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg aagtattaag
    38161 ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc aatttggcga
    38221 tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg aaacaatcaa
    38281 tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg gagaacccga
    38341 tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg aaatcactat
    38401 caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac cgacaaaccg
    38461 agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga aaaaaggcga
    38521 taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg aaacggccat
    38581 cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag acactatttc
    38641 gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg gggaaacatg
    38701 aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt ctacatggaa
    38761 ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca aatatcaaac
    38821 caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac ttatatcgga
    38881 aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt aactagcgga
    38941 tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt tgaagcgaac
    39001 atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc tgatatcgcg
    39061 ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat ctatactaat
    39121 ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga ggatatgaat
    39181 tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt tgatcaaaca
    39241 acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa actattgcca
    39301 tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc tgattgggaa
    39361 tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct tagaagagaa
    39421 gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca gctgctacta
    39481 catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc tgattggcaa
    39541 aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga tgaattggag
    39601 aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca tccggcgatt
    39661 gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga ccgtttcatg
    39721 gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt agtcttattg
    39781 caagaagaaa acggagaaag tagtgatagc agtgatcgtt aacgaagagc tgtttcaact
    39841 ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag agtaaaacga tggcttctta
    39901 tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga aagaagcgtt
    39961 cttgtctaag aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg cacctgatta
    40021 tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg atgagcatgt
    40081 tgcagctttc cgatatagtg aaacacagct gcaaagtctg ttggatcaaa ttgtccaagt
    40141 acttgccgaa gaagtttcta atgaaatcaa agtagatgca ggtagcccgt tttttgaaac
    40201 gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt agacaatagt gaatttgaaa
    40261 taacaaaacg acgcgggctg atcgtatggg tctacagctt aaaacaatta aagaatctga
    40321 aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg ctttacttaa
    40381 atgaagagac attggaagaa acagaagaac gaatccgtaa attacatttt gttcgaaacg
    40441 tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata ggaaacaaaa
    40501 cagtattaaa agacgaaggt ttcgaaattg aagagctaaa tacagaaatc cgattagctg
    40561 aacatcttta aaaatctctt atgagcagtt tttaaataaa aaataacttg gataacgatc
    40621 gatttttgat cattgtccaa gtattttttt ggtatactag taccagtacc atttctaagg
    40681 agtgagtaga tgcggataat atcaggaaat tatggcgggc gccgtttaaa aagtttagca
    40741 ggggcgaaca ccagaccaac tacagataaa gtaaaagaat ccatattcaa tatgattgga
    40801 ccatattttg atggagagac agtattggat ctgtttgcag gaagcggtgg tttagctatc
    40861 gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta ccaagcgcta
    40921 aaaatcatca aagaaaacat cgaaattacg aaagaaccag agaaatttac cacaataaaa
    40981 cttgatgcgg acaaagcaat cgaacggcta gcagcggaga aacaacaatt cgactacttg
    41041 tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc aaatccataa aatggaagaa
    41101 ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa cggataaaac agtcgaattg
    41161 ccagaaactg tggctggatt ccatcaaata agaaaacaga tttatggcat aacggccgta
    41221 actatctata gaaaagaggg aaatcaatga aacgtgcatt attcccagga agttttgatc
    41281 cttttacaaa aggtcactta gatacggtgg aacgtgcggc taaattattc gatgaggtag
    41341 taattggtgt ttttatcaat acttcaaaga aaagtttgtt tcccccagaa gaaagaatga
    41401 cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat caagaaaatc
    41461 aattgacggt agaaacagca aaagagattg gtgcagatgc gttgatcaga ggcatacgaa
    41521 gtatcaaaga ctttgaatat gaacgagaga ttgctcagat gaatcatcat ctatatgatg
    41581 aattggaaac tgtcttctta ttagcaaaac cagaatatag tcacgtgagt tccagtattt
    41641 taaaagaagt actgcacttt ggcggagatg tgagcagcta cttaccgcca gtgatcaacg
    41701 aagctttagc tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa aacagtactg
    41761 attatcatat ttggaatgtt gatcattgga tttgtagtag ttcccttgcc gtattatgta
    41821 gaatcaccgg gagccacgat cgatctgaaa gaactaatta cggtcaatga taaggaagat
    41881 aaaggttctg ggtctttctc tcttacctct gttggtatca ggcaagctac aggtttctta
    41941 gctttaaaag ctaaaatgac cccttttgaa gaattgatca gtgaagaaga attgactggt
    42001 ggggcgacaa atgaagaata taatcaaatg caacagtatt atatggattc ttcccaaaat
    42061 gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt ataaaatgga attcaaaggc
    42121 gtatatgtca tgagtgtaga gaaaacatcg aatttctttg gcaagctttc agttggagat
    42181 acagtaacaa aagtagacgg gaaaagcttt caaagtacag aagcatttat ggattatgta
    42241 aaaagccaaa aagttggaca aacaatcgaa atcagttatt tgcataatgg tgaagaaaaa
    42301 acagcttctg gtgatctgat cgagttacca acagataaaa aggcaggaat aggtatcacg
    42361 cttactgacc ataccgagat cgaatcagat acagatgttc gtattaattc tggctctatc
    42421 ggtgggccat cagcgggatt gatgttcact ttagaaatct atgaacaagt cactggtaaa
    42481 aacttgcgac atggtaagca aattgcagga actggaacga tcaacagtga aggagaagtt
    42541 ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg cgtagagatt
    42601 ttctttgcac cagatgatac gatttcagac gatgttaaga aagaatatcc tgaaataaaa
    42661 accaactacg aagaagcaaa agctgccgct aaaaaattga attcctcaat gaaaatcgta
    42721 ccagtaaaaa cagtacaaga agcgcttgac tatctagaga aaatgaaatg aaactaaata
    42781 attaaagagg ggttgtgaca cgagttctgt cacaacccct ctttttcgtc aaaaatcttc
    42841 acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta gatgttgaag ggacgatttt
    42901 gccagagctc actgtttctt caattttaaa tgaaaggagt aatgaatgat ttttactggt
    42961 cattctttga aaaaagaaca atgatagaaa aaatcagcaa gaaaatacta gcttcaccca
    43021 aattgatcat ttggatgtct atgatagttc ttatcttttg tatcgcaagt ggttttttat
    43081 tatcaaattt gctattttat agagaaacag aagagactgc tgcaaaagaa atactttctt
    43141 cgtttagttc cagtggagaa gatccatcag ctgaaggaaa cgatgtagaa gaggatgctc
    43201 gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa ggagcctggt atctattcct
    43261 tttcttcaga agaacgggtc tatgatgtac taaagcgtgc aggtggatta ttagaagaag
    43321 cggatagcga tcgaatcaac ttttctgcga agatagaaga tcagcaagtc ctatatatcc
    43381 ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt ggcatcctcg gaaggaaaac
    43441 agcctacagc agattccgag cctcccaaaa taaatatcaa tacagccact ccatcagaac
    43501 ttcagcagat tcctgggatc ggatcagtca aagcgcaaga aatcattcga tttagagaag
    43561 aaaacggatc cttccagaaa gtggaagacc tgcaggagat ttctggtatc ggtgaaaaga
    43621 cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa tgattcaaaa tcgactggca
    43681 cgagcaataa atggacgcta atggatacta agaataatcc aattctaaga gcttggcagg
    43741 acgaggaggt agtatttttg gataggaaat agcttgattg ttcgcaaaga aacattaaaa
    43801 aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg ggattcatat tatgaatacg
    43861 gctgtcagcg gcaataacag tcaagctcta cgagaacgct ttgccaaaga tgtacttagc
    43921 tatacagcag ataaagtatt tattctcatt gggacgaatg atctagcaga gcataaacaa
    43981 ctttctaaag aaacgtacca aaaaatctgc agtggatgat agttcagctg aaaaaagact
    44041 atagttctga tcatgctttc tttatcacgc caccagcagt cgatgagaca aaacaaaaat
    44101 atcggaataa caagttgatc aaagaatata tctctgttct cacagaagta gcaaaacgcc
    44161 atgattgtga agtatcgatt tttatacgga atttcttgaa caaatggatt cgaagaattt
    44221 ttataatggg ctaatggatg acggacttca tttcggcagt gaagggtagg atcttttgtc
    44281 agacaggatg attcgttata tttaaagaat attttgttta agcaatgagt actttttaat
    44341 caacttgaaa aaacggagga atataaaaca tgttagaaaa atcttcaaaa gcatgcgata
    44401 cgaataaaga aaaagttact gataagtcac ataatgaacg cattccttgg gaccagtact
    44461 ttatggctca ggcagtactg ttatctttac gtagtacgtg tacacgttta gaagtaggag
    44521 cgacactcgt tcgagaaaaa aggatcattg ctggcggcta taatggagca gtcagtggag
    44581 atgtccactg catagatgaa gggtgttacg tcgtggatgg acactgcttg cgaacgatcc
    44641 atgcagagat gaatgcactc ctacagtgtg caaaacttgg tatcccgacg gaaggttcag
    44701 aaatctatgt cacacatttt ccatgtttgg cctgtacaaa ggctttgttg caagctggtg
    44761 tgaaagggat caattatctc catgactatc gaaatgatcc ttatgcccaa gcgttgatcg
    44821 aacaattagg tgtatctgta caccaagttg ctttagactc acagcatttt tcaaaacttc
    44881 aaaatgaatt aacaggaaaa catgtctagc gttttagaaa ggcatgttcg aaatcggttg
    44941 atttttccgg caattacgtc gatgattatc tgtttgttgt attacactga caattggggt
    45001 tatcgtttgc ttcttgccgg aattttttta tggttttcgc taaaagaagg acgttttatc
    45061 gcgtacttgt gtattttgtg tagcataacg gtatccattt cttataggct acaagagtat
    45121 tcatttttag aggaaaaaac aacatctgcg caaattactg tatttacaga tacgattcgt
    45181 gcaaatggta attttttgac aatggaagga aggttatcag agaataaaca aaagctccag
    45241 ctttcttatc aagtaaacac agaagcagaa ttgaaaacat ggcttaactt ccgaggaaag
    45301 ggaatagaat tgactgctga aggcgagttc atctctccag caaaaaagcg gaatctttat
    45361 aattttgacc aagaagctta cttcaggaac catagaattt ctggacggtt ccaaattcaa
    45421 aagattgaac agatcaccat gcaaaatcac tggcgaaact ggcttcagag aaaaagagga
    45481 tcttttatct cctttgtcca atctgaattt ccgcaaaaaa caagtgtata tattaattct
    45541 ttattatggg gctataaaga tgcaggattc agagaaccag aagaaatttt tagggagagt
    45601 ggtttgctgc atctgttcag tctatcgggt ctacacatcc aattttattt agggtggctg
    45661 tattatttat ttcgacgtat cggatggcca ctacacgtca gtattattcc gttgagtcta
    45721 ctaactatct gttatgtttc tattgcagga agttctattt ctgttttgcg tgctagtttg
    45781 ctgtttttat tgcgattgct agtcaaattg ctgaataagc attattccac catggactta
    45841 tttgcagttg tcttttggct tcttttacta tttgatcctc gatttctttt tcatgcgggt
    45901 ggacagctta gttttttcat gagttttttg tttatcctga tttcttttga tggagattgg
    45961 ttgaaaaaaa cggcctctca cgtcctgttt actatgatca cgatgccgtt gattgtatgg
    46021 catttttatg agtggcctat tttaggtagt ctttttacat tactgattgc accggtattc
    46081 aagtttcttt ttcttcctgg tgtgttattt ctcacactct tcaatcgctg gctgccggaa
    46141 actttacttt tgctgataga agagggatta atcttatttg agcgaataat cgatttttct
    46201 tcagggtcgt ctctcattat aggacgtttg ccccttgtgc tatctgggat gtatattttc
    46261 ggattactga tttgtatgga taggttgaaa gaacaacctg tgaaacactt gttttatgtt
    46321 ggtctttttt cttttatttt tttgactttg ccatttttac gcttttcttc aacgatcgct
    46381 tttatcgatg tcggtcaagg agacagtatc tttttgcaaa gtccttttcg aaaagaaacg
    46441 attttgatcg atactggagg tcagttacac tttgaaagag aaaagtgggc gcaaggtatg
    46501 atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa aaggtcaggg gataagtgta
    46561 atcgataaac tcatcttgac acacgacgat acggatcatg tgggagaatt acccacgtta
    46621 tctcagcatt ttactatcaa aaaaatttat atcggttggg gcgctggacg aaatgagcca
    46681 ttacaaaggc atttatctga agctcaaaaa aatggtactg agatcattga aatcaaacat
    46741 ggggacagga tcagcggtta ttatgatctt tatgtattga ctccttttga aaagggagag
    46801 ggaagaaatg aagattcgat tggtctatgg atggaatata acaataggcg ctttttattt
    46861 ttaggagatt tgaatcaagc aatggaaaaa caattgcttt tgatttatcc taatctgaaa
    46921 gccgatgtag tcaaattagg tcatcacggt agccgtacgt caagtaacac agattttctt
    46981 tcccatattg aagcaaaaca tgggattata tcctgcggcg taaataatcg ttatggtcac
    47041 ccgcacagcg aagtattgga gacactagaa gaaaatcaga taaaaacatg gcgaacggat
    47101 caacaaggaa tgatcaccta tcgctggcat ccagtttttc atcctcatgg gctagtggaa
    47161 acaatgatag actagtaatt ctcatctgct catggtagga taatttttga aagtggtgag
    47221 taatttgagt atacaagcag agttacaaaa aatacgtaaa gaaaaacttg catcttgtta
    47281 cttgatttta ggaacagaaa agtttctcca agatcaagtt cggacagaaa ttgtcaaaaa
    47341 aatccaatta gagggcgaag atgacttaaa ttttttaagt tttgacatgg aaaatgcaag
    47401 tttagatgag gtagtagctg aagcagagac gttgccgttt ttcggagatc aacggttagt
    47461 attcgtagag aatccttatt ttttaacagg tgaaaaagta aacaatggaa tcgaacaaaa
    47521 tactgattta ttgacggctt atttgaaaga accattagaa tcgactgtat tagtattctt
    47581 tgcgtcatat gaaaagttgg atgaacggaa aaaagtaacg aaacaattga aaaaaacagc
    47641 aattatcatc aatgtccaac agttgaatga aaaagaagta cgtcaatatc tgatgaatac
    47701 cttagagaat tcctcaatca aaatggaacg aagtgcaatt gatttattct tgaggttgac
    47761 cgacttagat ttatccaaaa tgatgagaga gttacagaaa ttggtccttt atggacaaaa
    47821 tcaacagcaa atcacggtaa gagaagtaga agaattagtt cctaaaacgt tagaacataa
    47881 tatttttgac atgacccaat atattttaaa agggaagacg gaacaagctt tgcgtttata
    47941 cgaagattta gttatgcaag gggaagaaac aatcaaaatt aatgcgattt tgctttctca
    48001 attacgttta tttttacaga caaaatttct tgtaaagatt ggttatcagc aggcaaatat
    48061 tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt gccatgcagg aagtaaaaaa
    48121 attcgatgaa catttgctgg tacagttgtt tgatcaatta gttgagatgg attatcaaat
    48181 caaaacggga caaatcgaaa aagaattaag ttttcaacta ttcgttttgc gcacagggca
    48241 gttgttaaga aatcgataag aagaaagacg agggcaaagc atgcgggaag gtcgtttgtt
    48301 tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc gattctttta tttagcgatt
    48361 tttttgctta aacgagattt gtcgcggtta gctttgtttt tgtggattag accttttgat
    48421 tcagccatat caatagcttt aacagcttct ttatataacg catctacgtt gtcagcacct
    48481 gaagctacag catcttcaaa ttttttgatt gcagtacgca tagcgcttgt ttgagatgaa
    48541 tttttcacat tagcgttttc gcttgtacga acacgtttga ttgcagattc aatgtttggc
    48601 attagtttca cctccgataa cttaagttct tacgcgtgtt accgcataat tcaacattca
    48661 ttattatacc taaaggcagc gtgctttgca atatgaacat gacaagtttt tttccttaac
    48721 agtcttttat taggattttt tttcagtaaa aacggatagt acaagctgaa aaaaatcaaa
    48781 aagacgctat tgtgggaggc aatagcgtca aaccaaacta gatttttatt tatctattta
    48841 aacaaagttt gaatgggaat gtttggtact aagttattat ataacaaaga gaaccgtttg
    48901 caaagttttt tttgaaaagg gatacgattc cctgttgttc tttgtttttc tattaagaaa
    48961 ctgcagttat tttataacca gtatcctttg atcagtaaag gctattctgg tgtaccgatt
    49021 attttaatgt attcatcgat attttttatc aacatttttt ttatgaccaa ctggttccga
    49081 cggttccatt tgccacttac ttccagcaca tatttattta ggggcaaacg taagatatat
    49141 tttgccaaat agcgatcttt aagcaggcca ttgatcacac ggtcctcaag ctgcaagttg
    49201 atcccagtca gcatcattgg ctgttcttgt aaaacgtacg tatcaattaa aagaccttca
    49261 aaagtttcca caagcgtctt cctttctttt caaagtaacc tgaaaacagg cactaattct
    49321 tccattttga tattcgttga agtggatgcg atcattcttt cttttgtcca ctgtaatcta
    49381 gtataacaaa aaagagcaga agagccaaaa agaaaaaaga gcatcgaatt ctataggaag
    49441 tctcattggc acggcctaaa ttattacaaa acattgatta aaaaaagccc ctgcatcgca
    49501 ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag cagtatcaaa cacattttgt
    49561 atcaaatcag gaaataatag ataagctaag cccagaatca aaagtaccgc tagaagaaac
    49621 cagccgattg ttcctatgaa atactttaat ttttcgaaca ttgtatcacc tcgttgtcag
    49681 tatatcacgg atcagtaaat tgggggtgtt aagaatctcc taaaatagac aaaatttgtt
    49741 ctattttcct aaagcaaata cttgtaagat acagtaagtg ataccagaag caatgaccgg
    49801 tccggcggcg attcctctta agaagacaac acctaaaatc gtgccgaaaa caagtgcaac
    49861 agtgatttgt ggatcagaag caatcaatcc tacacccttg gaagataaga cagccactag
    49921 cccaccgcaa aaaacagcta cccagccaat aggggattta aaggcttcta atagatcacg
    49981 tagaccgatt tctcctgttg cgatagggat tagaatagcc gctgtgatga tcgtcacccc
    50041 aatattgatt cctttgcctt gaagccaagg gaaaaaggaa cgggaggctg gtatgagctt
    50101 cataatcaaa acaaaagctg ccgctatcat cagactttga tttttagcta aaattgctac
    50161 aagtaaaata attcctaaaa aaagccaact ttccatgaaa tatattcctc actttcttcg
    50221 aaacaatcat aacatgtttt tagaaagtta caaaagtagg aattcatcct tcaagaccta
    50281 ttcataagct gtttttttca taaaataaac aaaatcataa aaaaacactt tgcttatttg
    50341 acagaagaca gccgcgcttt gtatagtgga caagacttac atttttttga agaatcagaa
    50401 agatcaggta aaggtcttag taaacgcaag ccattcagaa taaccaatat tgtgcttcct
    50461 tcgtgtccta caactccaag aggtagattt aaaaattgca ggaagttaga agcaatcaac
    50521 aagagaataa caagaatcga aaaaataata ttttgtttga tgatccgccg catttttaaa
    50581 gatagccgat gactgtagac aaatttattt aagtcgcttt tcattaagac gatgtctgcg
    50641 atatccatag cggcatctgt cccttgcccc atagcgacac ctaaagaagc ggctgctaaa
    50701 gcaggcgcat cattgactcc atcaccaacc atcgcattta cttggtaaag ttcattctct
    50761 tttttgacta gttgtgtttt ttcttccgga gtacagcttg catagtaatg ttcaatccct
    50821 acttgttcag caatggcctt tgctgtgcca gaatggtcgc cggttatcat acttgtatgg
    50881 atgttttggg atttaaagta ggaaattact tgttgggtat ttgcttttgg aatatctagc
    50941 agacctaaaa cagcaattaa ctgctgatct ttagaaagat aaatcacggt tttgccttga
    51001 ttttccaaac gttcgatcat ttccgcaatc tctttgctga ttatcatcgt ctcaggatca
    51061 tatgcgtgct tgccaacttt ccattgcgca cctaaatagg tagtttgtaa tccaaaacca
    51121 gtaatttctt ctattggtaa gtattggatt tcttcaggta ttgtgcaatc gaaatattgc
    51181 aaaatcgcat gagcaagtgg atgcgtcgtt ttttgttcca tagcaacaag aagttgttgt
    51241 gccaaatcct tttcatcaaa gaaaaagctg tcagttacaa ctggttttcc tttagtgagc
    51301 gttcctgttt tatcaaaggc aatcgctttt aattcagaaa gcttttctaa atggataccg
    51361 cctttgatta aaatcccatt ttttgcagaa tttgaaagag cagctagaga agctggtgta
    51421 gcagatgcga caagcgcaca aggagaagcc acaactaata ataccattcc tcgataaaag
    51481 ctttcatccc aactccaacc taggaaaaag tgggggagaa ggatcattag aggaacagca
    51541 agcaaaatca attttacata attattttcg attcgttcga taaatgaagc tgtttgagtt
    51601 ggcgtattct gcgcttcttc tactaattgg ataatcttag caaagacagt ttcggaactc
    51661 gtttttgtta ctcgaatcgt gatgggattt ccttgattca gcgttcctgc atacatctca
    51721 tcgcctaaat atttttctac aggcacagat tcaccgctta atgcagactc atcgatcgtt
    51781 gcctgtgtgc tttctaaaaa gccatctatc ggaacagctg ctcctttagc gacaaatacc
    51841 aagttatcca cctttagttg agaaacatct acttcttgca tttgtccatt ttcttttaat
    51901 aattgggctt tttgtggttg aagattcatc aaagcagtaa tttctttttg acttttatta
    51961 gttgtgtatt cctctaatgc accgcttaaa caaaagatga atgtcaacat tgctccttca
    52021 aagtaattac cgatcaaaca ggcaccaata gcagctaaag ccatcaataa atccacattc
    52081 agtgtccgat cattccacaa ttctttcaag ccttccgatg tttgtttata tccaccgatg
    52141 ataatggcac tgatataaaa aagtggggtg atggtaagag agaatcggtc gaagataaaa
    52201 ccgaagatca taaataaaag gcagccgatt gttgctgcaa aagctttttt ctgattagtc
    52261 atcgttatta gacctccttg tcaaaacaag gatagcataa taaaaatgta attgataata
    52321 ataacagcta aatgagaacg ataatggtta tcatttggtg attaagcttg atgttgtttt
    52381 tattatttat aatgattatt attaaagaaa ataaaaaacc agaacatttc tcaaataaga
    52441 gaagtgttct ggttttttct acattgcaat aaaccaataa ccaatcaaaa tgataccaag
    52501 gataatacga taccagccaa aagcggtaaa gtcgtttttc ttcaaatagc ttagtaagaa
    52561 tttgatagct aagacggaaa ccacaaaaga tacgaatgtt cctacaagaa gaacaacagt
    52621 ttcttccatt ttgaatgaat tcccttcagc taaatatttg acgatcttca agccgcttgc
    52681 gccaaacatg acaggaatcc ctaagaaaaa tgaaaactct gtagcgacaa aacgcgaagc
    52741 accaatcaag atagatccta ggatcgttgc gccagaacgg gatgttccgg gaacaagtgc
    52801 caagacttgg aataacccaa caagaagagc tgcttggtac gtaaagtcat tcagagaagt
    52861 ccaacgaggt gttttgtttt tgttgcgttt ttcgacaata ataaaggcaa taccgtaaac
    52921 gatcaaaaca atggcaactg gtaagaactt gtggaaattt gcttctaacc aatcatccaa
    52981 aggcaaacca atgatgatgg ctggtatgca agcaacgatc actttaaacc aaagtgtcca
    53041 cgtatctttt ttttctaccg ttgatttact tggagaaaat gggtttaact tgtgaaaata
    53101 aagaacgacg acagccaaga tcgcccctaa ttggataacg acattgaaca tcgacataaa
    53161 ttgtgaactg gcatccagtt tgatgaattc atctgctagg atcaaatgac cagtactact
    53221 gatgggcagc cattccgtta ttccttcaat aataccgaga atcactgctt tgataatatt
    53281 tgcaaaaaac ataaattcct tccttcccaa ttaagataca tcttatagta taccgttatt
    53341 ttttgagaaa cccaatctgt tttttaatat taagaaaaac ttgagaaata aagcagataa
    53401 gtacacgaag agaaaaaaga agacaaagcc ctcgttcaga ctttgccttt tatgattctc
    53461 tggtaaaaat taaaatatcc ccattataga aacgagtcgc gactatgtgt tttcccgtta
    53521 tcgatataag acgtatcatt gatgccgtgg atagaaaatc gaccgtttgc ataagtgacc
    53581 tttgttacgc tgccatttaa aagcccagca cgaacaggct gtttaggatc gatcagatta
    53641 agcaaaaagg cgatcgtcaa accatgagat acgactaaaa catttccacc accatttttt
    53701 tcgcagtgat gggcgatatc ttcaaatcct gtccatacac ggtctcgcaa tgtttcataa
    53761 gtttccgccc acccagcggt atctaagttg tatacagcat tagcaatttg ttcaaaagaa
    53821 acttctgtgg taaacatttc atcatatgtt ttaaaattca atacacgagg gatcacaccc
    53881 cacatttcca tgtcatatcc accttcaaga gacccgaaac accattctct aatccttttt
    53941 tcaataaaat aaggaatttc ttttccctta gaatgttctc ctagaataat acgggcagtt
    54001 tcgattgcac gaccactgtc actagaataa gcaaatgaaa agtcgacatt ttttaacccg
    54061 cgacctaaat aatgaatgcc ttcttgtcct tgctttgtga ggggtgtatc acaccagcct
    54121 tgtactcgtt caattgtatt gaacatcgtt tttccgtgtc gtacgatata aagtgtagtt
    54181 tctgtcattt tattctcctc ctaagtcgtt ttaacgattg aaaaaaggat ttgtcctttt
    54241 ttcctgttca atcgtcgttg catcgccatg tcctggatag actgcaaatt catcaggtaa
    54301 tacaaacagc tgggtccgaa tactatgaag taattgttct aaattccctg tatacagatc
    54361 ggttctaccg atacttcctc taaacagggc gtcgccagaa acgacaaaat catcaaaaat
    54421 aaagctgacg cttcctatag aatgccctgg ggtagggacg acgcgaaaag acatgtttcc
    54481 taaagtataa gttttcattt caaattcata ttctgcaggc gagacaataa catcagcaat
    54541 atcatcatgt ctaccgagtc cagaaagatt caaaatcgga tcacctagcc atttttgctc
    54601 tagtgggctg acataaactg ggatttgata atacgcgcgt aaaggttcca ctgcaccgat
    54661 atgatcgtag tgagtatggg taagcaaaat cgctaccggc tgttggtttg tttttttgat
    54721 ttcttgtttg attcgttccg catcttctcc agggtcaatg atcaaaagtg cttcttcgtt
    54781 gtagaccaaa taacagtttt cttcaatcat tcctgttttt aatctttcga tatgcatgtg
    54841 cattcctcct tgatagccaa aacttctttc atccattagt ataacgcatt cattgctagg
    54901 aaacaaaaac aggtacttat ttctctttac ttatctgaat tatcagacta tatgaaaagt
    54961 atttggatat tctaaagaga aggaaagtaa gttttcatag cattttaata tgtccttgtt
    55021 acattagatt catccaaaaa tccaaattct atatcctata tccctgccta atcagcaggg
    55081 tgttttttta tgtttggaaa gagagctctt tttgctaaac taggtttttt caggtaatct
    55141 tagagagaaa gaggtggtgg aaatgatcaa acagtcaaaa atatctgtgc ggcacttagt
    55201 agaatttatt ttgcgacgtg gaagtatcga caatcgtaaa aaaagtaacc atactgcatt
    55261 agaaggagcg agaatccacc gcaagctcca aaaagatgcg ggagaaaact atgaaaaaga
    55321 agtcttttta aaaacaacag ttcaattaga tgaatattac ctgatcgttg aaggccgtgc
    55381 agacggtatt ttcaaaagag acggtattta ttatatagat gaaatcaaaa cctccgaacc
    55441 acgatttgaa gatttagaac cagaacaagt ggaacttttc tttcatcaag caagggtgta
    55501 tgcctatatc tatagtcaag aacatgattt acaagaaata aatctgcaat tgacttactt
    55561 ccagacggca gaagaagtga tcacgagaaa ggttgaacat cagacaagag agcagttaga
    55621 aacttttttt aaaaagctga ctgaagatta taaagaatgg cttgtttttc aagaaaactg
    55681 gcgcactgta cgcaatgcct ctttgatggc tttgaaattc ccccatgatg agtataggaa
    55741 aggtcagcgt gaactagcgg tagctgctta taaaacgatc cgtacgaaac agaaattgtt
    55801 tgtagaagca cctaccggaa caggaaaaac aatctctacg ttattccccg ctttgaaagc
    55861 tgtaggggaa gaagagggag aaaagatctt ttatctaacg gctaaaacaa taactagaca
    55921 agtagctgaa gatgctatga cagcattaaa agatacagga gctgaagtaa aaagtgtcac
    55981 tttgacggcg aaagacaaga tctgcttttt agatgagacg acctgtaatc cagatcagtg
    56041 cccatatgcc aatggttatt acaatcggat taatgaaggt ttatgggatc ttttgaatca
    56101 tgagaatcag atcactcgtg aagtcatcga aacttatgca agaaagcata cactttgtcc
    56161 tttcgaacta tcattagatg tcagtatctg gtgtgacgta atcattgggg attacaatta
    56221 tttatttgat ccaactgttt atttaagacg tttttttgaa gatgaaaaaa acgaggacta
    56281 tctgtttttg attgacgaag cacataattt ggtgaatcgt tcccgagaga tgtattcggc
    56341 agaactatct tatgaaaaaa cgaaacgaag taaggaagct ataccaaagg aattcaaaaa
    56401 acttcatcgc cgtttcaaca aactattaaa agaatttgat tcgatccgag aaatagcaaa
    56461 agaagatcat tgggattatc atcatcaaaa agcaccggct gaatctttag taaaggcagg
    56521 ttatcaatta agcgaaaaaa ttaaagaatg gctggcagaa tttcctgagc acccatcaca
    56581 agaacaattg cttccttatt attttgattt gcttcatttt ttgaaggtca gtgaatttta
    56641 tgatgatcac tacgaaacaa cagtcgaaaa aacgtatcgc gatctgatag taaaagagtt
    56701 ttgtattgat ccaagcttgt ttcttgagca gtcgttggat aaaggaaaaa gcagtattct
    56761 tttttctgct agtttttcac cactctctta ttatcaggaa acactggggg gacaaaaaag
    56821 tctggcttac aaactgccga gtcctttccc agaaggaaac cagcaagttt tcatcgcaaa
    56881 ttacctagag acgacttatc gaaaaaggga acacagtctg gcaaaattag tagagacgat
    56941 tcatgctttt gctgatggaa aggtcgggaa ttatttcgtt ttctttcctt cttatcaata
    57001 tttagatctt gcggtggaag cattcagaca gcaatatccc gacagcaatg tgctaatcca
    57061 agaaacggac atgaatgagg aagaacgcga acgattttta acaaaatttc aaacaaatcc
    57121 cgctgaaaca ttgattggat tttgcgtact tggcgggatt ttttcagaag gaatcgattt
    57181 gagaggggac cgattgatcg gcagtatgat cgtaggtgta ggtttgccgc agatgaatca
    57241 tgagcaggaa ctgatcaaat catattatga tgagaaggaa caccttggat ttgcttacgc
    57301 ttatcagctt cctggaatga acaaagtctt gcaagctggt ggtcgagtga ttcgtggcat
    57361 ggcggatcaa ggaatcgttg tattggcaga tcaacgattt tctagttttt cttatcgacg
    57421 tttattccca caacattggc aaacagcaag agaagtacga agtgttgggg aattggaaga
    57481 aggaatacag cgtttttggt tatcaaaaga agcagtaaat caaaaaaacg aggaatgaca
    57541 gacgtcagcc tcgttttttg attaacgata gttagtgaat tgcaactcga ttggtaagtc
    57601 aagtttacgt aataaagcaa tcacagcttg tagatcatca cggttcttac ctgtcactct
    57661 aatctgatct tcttggattt gtgcttttac tttcagtttt tcatttttga tagctgtagt
    57721 gattttcttc gcgttttcac gatcgattcc gttgaccaaa tcggcagttt gtcgagcttt
    57781 gccacctaga gcgtgttctg attcggaaaa atggatattt ttgattggca catttctttt
    57841 gatcagctta ccaaataata catctttgat ctgttcgatt ttgaaatcat catcgctcaa
    57901 tacaactaga ttgctgttct ctaatttgat ttctactgta gaatccttga agtcaaagcg
    57961 attggacaat tcttttgttg tttgctgaat cgcatttttt acttcttctg tattgacttc
    58021 tgatacaatg tcgaagctag catcttttgc cataggaacc ctcctgatat acatataaat
    58081 atttaccata catctatctt atcataggat tagggcagat tcttacctgt ttgctaaaat
    58141 tcttctcatt tgagttcgaa gtattttatt ttaatagata aactgttatt cttacatttg
    58201 gaataccaaa gtaggggaga ggtgcttgtg agtaaaatta tgactgcgta tcataaaaat
    58261 gaaagattaa agaaaatagt ggttattttg ataaccggat tgaccgctgc ggtcgcttta
    58321 aattattttt tgattccagc caatgtcttt tctgcaggga tgaatgggat cgctcaaatc
    58381 atttcctcgc ttttatatga atggtttcat atcaagatag acacaggaac atttattttt
    58441 cttttgaata tccctgtgtt tgtattaggg tttctcaaag taggaaaacg agcaacgatc
    58501 cttagttttg tgaatgttgt atgtgtatcg gtgatgacga tgctgcttcc agtcggacag
    58561 atcactgata atattttgat gaatgcccta gtaggcggtg ttttattagg tcttggcgta
    58621 ggattatctt taaaaatggg ctttacaact ggtgggatgg atatcatctc tcttgtactt
    58681 tcacaaacaa ctggaaaaac agtcggaaac tacatgctga ttctaaatgg tatcattgta
    58741 gtcatcgcag gcttcttctt taactgggaa agtgccttgt atacgattat ttccatttat
    58801 gcaatgactc aagtaattga tgccatccac acgagtcatc aaaaagtaac agcaatgatc
    58861 gttaccgtca agccagaaga ggtaacagaa gaactagcaa aacggatggt acgtggaatg
    58921 acattactgc cgtctgtcgg tggctattca aaaaaacaag ggaacatgat catgatggtc
    58981 atcacccgct acgaaatgta tgatcttgat cagatcgttc atgaaatcga cgacgatgca
    59041 tttatcaata ttttaccaac tcattcagta tttggccggt ttgcaaatga aaacgaacaa
    59101 aaaatgtatc gaacaactgg cgtattgcca gaaataaaaa atcgttcaca aaaaaaatga
    59161 ggaatcaggt ttattttaga ataaatttaa tctattacat ctaataagaa catttttata
    59221 aatttaaacg agaatcttac ttctaaattc tcatttacaa gcagctaaaa aaagagtatg
    59281 ataaataaaa atcgtgttaa ggagtgtcgt acgatgagcg aagcaaacca agaacggtct
    59341 ggacaagaaa tagaactaat caaagaacgt attcttgctg ccttggaaat ggtgattgat
    59401 cctgaattag gaatcgacat cgtcaattta ggcttgattt atgatataga attcaatcct
    59461 gaaaatggag aaacagtcat taagatgact ctaacaacta tgggctgtcc attagcagat
    59521 attttaacag aatctatcca tgacgcatta aaagaagtgc cagaagtaac gaaagcagaa
    59581 gtcaaactag tctggtatcc agcatggacg acagataaga tgagccgtta tgcacggatt
    59641 gcgttgggga tacgatagaa atcttggctg actagctatg agaagcaaat aggaaaaaca
    59701 aaaaagcctt ttatcctttc tagaagaaaa gagataaaag gtttctttgt ttataaaatt
    59761 ttgtctgcta tattataagt taattaaata aatgtgttct attgataaag gacattcaaa
    59821 tccctttcct tttggaatcc ttctttttta catcagtttg attttcgtat agtattcttt
    59881 ctcatcttca cgataaatcg cagccccatt tttttgttgt gctttaagac tgccaagatt
//
LOCUS       DAZKCZ010000004        59653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000004 DAZKCZ010000000
VERSION     DAZKCZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.004"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..929)
                     /locus_tag="ACG8KN_000174"
     CDS             complement(81..929)
                     /locus_tag="ACG8KN_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9187266.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1153..3093)
                     /gene="thrS"
                     /locus_tag="ACG8KN_000175"
     CDS             complement(1153..3093)
                     /gene="thrS"
                     /locus_tag="ACG8KN_000175"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9187267.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3430..3609)
                     /locus_tag="ACG8KN_000176"
     CDS             complement(3430..3609)
                     /locus_tag="ACG8KN_000176"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFP9187268.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3656..5479)
                     /locus_tag="ACG8KN_000177"
     CDS             complement(3656..5479)
                     /locus_tag="ACG8KN_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9187269.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGVKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5757..8036
                     /gene="helD"
                     /locus_tag="ACG8KN_000178"
     CDS             5757..8036
                     /gene="helD"
                     /locus_tag="ACG8KN_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFP9187270.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETEIDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8129..8776)
                     /locus_tag="ACG8KN_000179"
     CDS             complement(8129..8776)
                     /locus_tag="ACG8KN_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187271.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8955..9674)
                     /locus_tag="ACG8KN_000180"
     CDS             complement(8955..9674)
                     /locus_tag="ACG8KN_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9187272.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9805..10956)
                     /locus_tag="ACG8KN_000181"
     CDS             complement(9805..10956)
                     /locus_tag="ACG8KN_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFP9187273.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11173..11913)
                     /locus_tag="ACG8KN_000182"
     CDS             complement(11173..11913)
                     /locus_tag="ACG8KN_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9187274.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11917..12273)
                     /gene="rsfS"
                     /locus_tag="ACG8KN_000183"
     CDS             complement(11917..12273)
                     /gene="rsfS"
                     /locus_tag="ACG8KN_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9187275.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(12297..12890)
                     /gene="yqeK"
                     /locus_tag="ACG8KN_000184"
     CDS             complement(12297..12890)
                     /gene="yqeK"
                     /locus_tag="ACG8KN_000184"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFP9187276.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12874..13524)
                     /locus_tag="ACG8KN_000185"
     CDS             complement(12874..13524)
                     /locus_tag="ACG8KN_000185"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9187277.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13534..13845)
                     /gene="yhbY"
                     /locus_tag="ACG8KN_000186"
     CDS             complement(13534..13845)
                     /gene="yhbY"
                     /locus_tag="ACG8KN_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9187278.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13861..14970)
                     /gene="yqeH"
                     /locus_tag="ACG8KN_000187"
     CDS             complement(13861..14970)
                     /gene="yqeH"
                     /locus_tag="ACG8KN_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFP9187279.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14967..15494)
                     /locus_tag="ACG8KN_000188"
     CDS             complement(14967..15494)
                     /locus_tag="ACG8KN_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFP9187280.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15721..16506)
                     /locus_tag="ACG8KN_000189"
     CDS             complement(15721..16506)
                     /locus_tag="ACG8KN_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9187281.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(16499..17368)
                     /gene="accD"
                     /locus_tag="ACG8KN_000190"
     CDS             complement(16499..17368)
                     /gene="accD"
                     /locus_tag="ACG8KN_000190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9187282.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHML
                     LELHTQKGWIDRG"
     gene            complement(17369..18748)
                     /locus_tag="ACG8KN_000191"
     CDS             complement(17369..18748)
                     /locus_tag="ACG8KN_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9187283.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPTRHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18756..19175)
                     /gene="fabZ"
                     /locus_tag="ACG8KN_000192"
     CDS             complement(18756..19175)
                     /gene="fabZ"
                     /locus_tag="ACG8KN_000192"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9187284.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19200..19676)
                     /gene="accB"
                     /locus_tag="ACG8KN_000193"
     CDS             complement(19200..19676)
                     /gene="accB"
                     /locus_tag="ACG8KN_000193"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9187285.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19679..20914)
                     /gene="fabF"
                     /locus_tag="ACG8KN_000194"
     CDS             complement(19679..20914)
                     /gene="fabF"
                     /locus_tag="ACG8KN_000194"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9187286.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20933..21670)
                     /gene="fabG"
                     /locus_tag="ACG8KN_000195"
     CDS             complement(20933..21670)
                     /gene="fabG"
                     /locus_tag="ACG8KN_000195"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFP9187287.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21682..22599)
                     /gene="fabD"
                     /locus_tag="ACG8KN_000196"
     CDS             complement(21682..22599)
                     /gene="fabD"
                     /locus_tag="ACG8KN_000196"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9187288.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22706..22930)
                     /locus_tag="ACG8KN_000197"
     CDS             complement(22706..22930)
                     /locus_tag="ACG8KN_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9187289.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22983..23945)
                     /locus_tag="ACG8KN_000198"
     CDS             complement(22983..23945)
                     /locus_tag="ACG8KN_000198"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9187290.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23955..24395)
                     /locus_tag="ACG8KN_000199"
     CDS             complement(23955..24395)
                     /locus_tag="ACG8KN_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9187291.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24701..25666
                     /locus_tag="ACG8KN_000200"
     CDS             24701..25666
                     /locus_tag="ACG8KN_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9187292.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
     gene            complement(25723..25953)
                     /locus_tag="ACG8KN_000201"
     CDS             complement(25723..25953)
                     /locus_tag="ACG8KN_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFP9187293.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26039..26926)
                     /locus_tag="ACG8KN_000202"
     CDS             complement(26039..26926)
                     /locus_tag="ACG8KN_000202"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9187294.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27015..28238)
                     /locus_tag="ACG8KN_000203"
     CDS             complement(27015..28238)
                     /locus_tag="ACG8KN_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9187295.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28255..29301)
                     /locus_tag="ACG8KN_000204"
     CDS             complement(28255..29301)
                     /locus_tag="ACG8KN_000204"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9187296.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDVEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29465..31192)
                     /gene="ptsP"
                     /locus_tag="ACG8KN_000205"
     CDS             complement(29465..31192)
                     /gene="ptsP"
                     /locus_tag="ACG8KN_000205"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9187297.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31192..31458)
                     /locus_tag="ACG8KN_000206"
     CDS             complement(31192..31458)
                     /locus_tag="ACG8KN_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFP9187298.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31937..34171
                     /locus_tag="ACG8KN_000207"
     CDS             31937..34171
                     /locus_tag="ACG8KN_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9187299.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34319..34639
                     /locus_tag="ACG8KN_000208"
     CDS             34319..34639
                     /locus_tag="ACG8KN_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9187300.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34844..35068)
                     /locus_tag="ACG8KN_000209"
     CDS             complement(34844..35068)
                     /locus_tag="ACG8KN_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFP9187301.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35266..36846)
                     /locus_tag="ACG8KN_000210"
     CDS             complement(35266..36846)
                     /locus_tag="ACG8KN_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9187302.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37247..38623)
                     /locus_tag="ACG8KN_000211"
     CDS             complement(37247..38623)
                     /locus_tag="ACG8KN_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9187303.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38751..39839)
                     /locus_tag="ACG8KN_000212"
     CDS             complement(38751..39839)
                     /locus_tag="ACG8KN_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9187304.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            40011..40291
                     /locus_tag="ACG8KN_000213"
                     /pseudo
     CDS             40011..40291
                     /locus_tag="ACG8KN_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(40369..40902)
                     /locus_tag="ACG8KN_000214"
     CDS             complement(40369..40902)
                     /locus_tag="ACG8KN_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFP9187305.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(40996..42174)
                     /gene="mutY"
                     /locus_tag="ACG8KN_000215"
     CDS             complement(40996..42174)
                     /gene="mutY"
                     /locus_tag="ACG8KN_000215"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9187306.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42167..42964)
                     /gene="recX"
                     /locus_tag="ACG8KN_000216"
     CDS             complement(42167..42964)
                     /gene="recX"
                     /locus_tag="ACG8KN_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9187307.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43071..44453
                     /gene="rlmD"
                     /locus_tag="ACG8KN_000217"
     CDS             43071..44453
                     /gene="rlmD"
                     /locus_tag="ACG8KN_000217"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9187308.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44627..45205)
                     /locus_tag="ACG8KN_000218"
     CDS             complement(44627..45205)
                     /locus_tag="ACG8KN_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFP9187309.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45527..45997
                     /locus_tag="ACG8KN_000219"
     CDS             45527..45997
                     /locus_tag="ACG8KN_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFP9187310.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46105..47331)
                     /locus_tag="ACG8KN_000220"
     CDS             complement(46105..47331)
                     /locus_tag="ACG8KN_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9187311.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48085..48285)
                     /locus_tag="ACG8KN_000221"
     CDS             complement(48085..48285)
                     /locus_tag="ACG8KN_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9187312.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48419..49027)
                     /locus_tag="ACG8KN_000222"
     CDS             complement(48419..49027)
                     /locus_tag="ACG8KN_000222"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFP9187313.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49112..49582)
                     /locus_tag="ACG8KN_000223"
     CDS             complement(49112..49582)
                     /locus_tag="ACG8KN_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFP9187314.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49630..50544)
                     /locus_tag="ACG8KN_000224"
     CDS             complement(49630..50544)
                     /locus_tag="ACG8KN_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9187315.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50690..51493
                     /locus_tag="ACG8KN_000225"
     CDS             50690..51493
                     /locus_tag="ACG8KN_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFP9187316.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51671..52618
                     /locus_tag="ACG8KN_000226"
     CDS             51671..52618
                     /locus_tag="ACG8KN_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9187317.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52686..52979)
                     /locus_tag="ACG8KN_000227"
     CDS             complement(52686..52979)
                     /locus_tag="ACG8KN_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFP9187318.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(53007..53672)
                     /locus_tag="ACG8KN_000228"
     CDS             complement(53007..53672)
                     /locus_tag="ACG8KN_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFP9187319.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(53812..54444)
                     /locus_tag="ACG8KN_000229"
     CDS             complement(53812..54444)
                     /locus_tag="ACG8KN_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9187320.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54451..55518)
                     /gene="liaS"
                     /locus_tag="ACG8KN_000230"
     CDS             complement(54451..55518)
                     /gene="liaS"
                     /locus_tag="ACG8KN_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFP9187321.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(55515..56246)
                     /gene="liaF"
                     /locus_tag="ACG8KN_000231"
     CDS             complement(55515..56246)
                     /gene="liaF"
                     /locus_tag="ACG8KN_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFP9187322.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(56414..56893)
                     /gene="greA"
                     /locus_tag="ACG8KN_000232"
     CDS             complement(56414..56893)
                     /gene="greA"
                     /locus_tag="ACG8KN_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9187323.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57029..58204)
                     /gene="mltG"
                     /locus_tag="ACG8KN_000233"
     CDS             complement(57029..58204)
                     /gene="mltG"
                     /locus_tag="ACG8KN_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFP9187324.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            58369..59529
                     /locus_tag="ACG8KN_000234"
     CDS             58369..59529
                     /locus_tag="ACG8KN_000234"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9187325.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT        17267 a        12570 c        10058 g        19758 t
ORIGIN      
        1 aaacactttg atggatcatt tatttgtgga aaactacgtt ttgttattct aaaaaactta
       61 tataaaaaat agtaaggttt ttatttcgaa tccataaaat attcttggat tgcttctcgt
      121 gtttttttca ctttttcgct aagcttttcg tttttagcca tatagaaata aaaaatgata
      181 tcgatcgtag caaattgggc caaaagtgaa ttagttgccg cacttcgatt cgtgatttct
      241 tttggtctag atgtttgtac gagtacatcg gctatcttgc taagcgggtt atttccaaat
      301 tgcgtcaacg caatgatttc gatattcatg cttttcgcca gctcggctaa tgcgactaca
      361 tctgcacttc tacctgagtt tgaaacaagc caaagcactt tctttttctc attgctgaca
      421 agttgcggga gcaagacgtg gatatctttt tcaaaaatga ttggcttacc gacacgagac
      481 catttttgca gaatatcttc tactactaaa gaagaggcgc caaccccata taaaaaaaca
      541 gtctctgccg attccaaaca ctctacgact ttttggatcg tttcttcttc tagaatatct
      601 actgtttctt ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat agagtaaaag
      661 gactcatttg cttctatatc actaaaacct tcatcgattc cagtctgtag ttctgttgaa
      721 atcgtgattt tgaagtccgt cagactttga aaaccgattt ttttggaaaa acgtacaact
      781 gtcggcgcac tcaacccgga agcttctgct aattcattag ctgtcattgt agggaccttc
      841 tcaatattac cgataatgta gttggccaac tttttttctg ctgaagaata atcttccatc
      901 atactttcaa tacgactaat tacgctcata agttcctcca cccttttaat gatagtctat
      961 gtaagtatac catatcagac ccttattcac ggtcgtatac tggataaaaa acaaaaaaac
     1021 aatccgccca acagaatatc ttatcccatc aatcccacag attctattga gcatctgtgt
     1081 ctttgaggaa tacctgttat ctattgttcg gattgctttc tatctactac ttttttacag
     1141 gaaatttacc tattagtttt ctctgctgta atttttgact tctgcttcca tagcatcaat
     1201 aaagatattc aaatcttcta cagctgtttc tttacttcca taacgacgta cgttcactgt
     1261 tgcatcttcc aattccttgt ctccaacaac taattggtaa gggatctttt gtgtttgaga
     1321 ggcacgtatc ttatagccca ttttttcatt acgatcatca acttctacac gcaacccctt
     1381 catttgcaaa cgttctttga tttcataagc ataatcgcca tgtgcatcaa cagaaacagg
     1441 gatgattgtt gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt atacttctgt
     1501 taaataagcg acaaaacgtt ccattgtaga aacaattccg cggtggataa ctactggacg
     1561 atgcgtattt tccccgtctt ctccaacata tgtcaaatca aagcgctctg gcaataagaa
     1621 gtctaattgt atagttgata atgtttcttc tgtaccaaga gctgttttga cttgaacatc
     1681 tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta gttctaattc
     1741 atccatagcg gctttcagca ttgcttgggc tttttcccac attgcgtcat catcaaaata
     1801 tttatctgta ttgtttggat cgcgatagct caagcggaaa cgataatctg taatattgaa
     1861 gtcagcatat actgccacca tcaattcaag cgtacgttta aattcatctt tgatttgatc
     1921 aggacgaaca aatgtatgac catcattcaa cgtcatttct cgtacacgtt gtaaacctga
     1981 caaagcacct gatttttcat agcgatgcat cattcctaat tcagcgatac gaattggtaa
     2041 ttcacgataa gaatggatcg tgtttttgta gaccatcatg tgatgagggc agttcattgg
     2101 acgaagaaca agcatttcgc catctcccat atccattggc gggaacatgt cttcttgata
     2161 atgatcccag tgtccagatg ttttgtacaa ctcaacatct cccattattg gtgtatatac
     2221 atgttggtaa cctagactaa cttctttgtc tacgatataa cgttcgatcg tgcgtcggat
     2281 tgttgcccct tttggaagcc agaatggcaa accagaacct acttctgggg aaaccatgaa
     2341 caagtcaagt tcttttccta gtttacgatg gtctctttct tttgcttctt cacggagacg
     2401 gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac gttgcatcat
     2461 ttgattgttt gagtttccac gccaataagc acctgccact gataataatt taaatacttg
     2521 tatacggcca gtggatggaa catgtggtcc acgacacaaa tcgatgaatt cgccttgttg
     2581 ataagcagta atcacttctt cttctggcaa ttcactaatc agttctactt tgtacggatc
     2641 acttgcaaaa atttctaatg cttcctgttt tgaaagaaca cggcgttcga ttggcagatt
     2701 ttctttgacg attttcatca tttctgcttc gattgccggc aaatcttctg cagttacttg
     2761 gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc caacgccaaa
     2821 gtggatattt ggatagaggc gacgcatggc ttgggccatc aaatgagctg ctgaatgacg
     2881 gactaagcca agtgcatctt catggtctgg tgtgatgatc tcaatacttg catcttcctc
     2941 gattggacga gtcaaatcaa cgagttttcc gttcacttta ccagctaaag ctttttttgc
     3001 taagcttttt gaaatgcttt cagcaattgc caatgttgtt gttccagact caaattcttt
     3061 gactgcacca tctggaaaag taatttttat cataatagtt tcctccttga aatgttttga
     3121 ttgatcagat catcaagctg aaaaacaaaa aaatcctagc atctcttgaa aagagatact
     3181 aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc tcaagcgttg
     3241 taacggtccg accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc tttggttttc
     3301 tcctggaaaa gtttcagcct agcttttcct ctcttatcga agactctcaa attcagttgg
     3361 ctttctcatc aatcaattat gtgtttattt aaccactaat aaatagaaaa atcaagtggg
     3421 aaactaaact tattttttcc attccccatt tacaccgtat gttccttttt tcatctcatt
     3481 gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag cttccgtgat
     3541 atctttcatc atattttcta attgttccgg actgcgacct tctactaatt cgacatgtac
     3601 gaatggcata atgctctctc ctctttctga tcgttctttt ttcaactcga ttctatcata
     3661 ctatgtttgt cttgggtact gaatcaggaa aaatggctgg atcttttttt gctctcaagg
     3721 cactcaaaat agagaccgta tccacgactt cttgcaaaac agctccgaat aatgcgggga
     3781 ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat aaagatgcca atcagtacag
     3841 actgtttagc tattctcatc gtatctcttg aaatcactac tgcttttgct acacgcaaga
     3901 gatcatcttt caagatgacc gcatctgcac tttcactagc agctgtagaa ccatgagccc
     3961 ccattgcaat accgatatct gcagcagcaa gtgctggagc gtcattcacg ccgtctccta
     4021 ccatcgcaac aggtctttga tctttaggga tactttctaa aatggtcagt ttatcttgag
     4081 gcaagctacg ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt tgagcaatgg
     4141 cagatccatc cccggtgatc atcatcaaat ttttcacatc aagttttctt agctccttga
     4201 ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag gatcgttcct gcaaaagatc
     4261 gatcaattgt tacatagatt gctgtattgg acgtttctat ttcttcagct gaaacaaaag
     4321 aggcacgtcc aatctttact tcatgattat tgactactgc ttggattcct tgaccggtca
     4381 cttctttcag ttgactaaca ggatacagtt ggctgttttt cacgttgttc actaacgaac
     4441 gagctaagat atggctagat tcctgttctg cactagctgc atacagaagt agctcttctt
     4501 tttttactcc attgactggc tgaatcgcct ctacttccaa tatccctttg gtaagcgttc
     4561 ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt gttccagttt
     4621 ttacaacgat cccgttttta cttgaacggc tcattcctgc aactaaagca atgggcgcag
     4681 ctagaatcag tggacaaggt gatgccacta ccagtacctc agcaaatcgt aaagggtctt
     4741 ttgagaacca ccaagaaata ccaccgatca aataagcaat caacgtgaaa gggactgcat
     4801 aacgatcagc taaacggaca aatcgggcag gcttttcttt tgattcttcg actagtttaa
     4861 taatcatttg atactggctg tctgcggcac gtttttcagc gatcatttgg atcgatccgt
     4921 ctccatttaa agaccctgac ataatctgat ctccaggttt cttggtttcc gggcgggact
     4981 cacctgtcaa agaagattca tcgaccgaag attctccttt ggtcactcga ccatctgcgg
     5041 gtacaagttc gccaggcttg actagtaact gatcatcaat atttatttca tcgattacta
     5101 tatcctgcaa ttttccttct ttgaatcgat gggcaattct tggggtgtta tctaacaaag
     5161 actgcagttc tttacccgct cgtttattcg cgtaatcctc tagactatct ccgcccgtaa
     5221 gcatgatcaa tatcatcaaa ctagcccagt attctttgac ggctaatgtt gcaaggattg
     5281 cggtaatcgc taatacatct acgccatatc ttcctgaacg taacgtcttg atcatttcca
     5341 aaaacatcaa gaaggcgaga atccctccag taaatgcgac aatcgcaaat gcaagggccg
     5401 gctggtgaaa aacaaattca cttaataaag ctatcccacc aataagcaaa gtaagtaaaa
     5461 attttttgaa atgtgtcatt gttttttctc ctcttttatg taacttagag aggttagtaa
     5521 taaatttcca gctgcctctt tcatcttaag catagtcctt gactagcact ttttaaaaca
     5581 ataggcctta attgaaaaca actatcacta tcagcgtttc caaaattcat cagttctaca
     5641 aacaaaaata ttcagcaagg attacttttt cgattcatca gttaaaaagt accttttttc
     5701 gaaaaactat gctatacttt taattcgtta tttacggcgg aaggagcaaa catgcgatga
     5761 atgagaaaca actagaacaa acgcacgtgg atgaaacgat cgaactaatc cagatcgaac
     5821 aaaatatgct ggatacaaaa caacaagaat tgactcagga aatgaatcat tcgacaaaag
     5881 acacagctaa tcacaagatt cgagctggtt ccaatgaagc tttttatgaa tctgctgttg
     5941 aatatcgcca acatgagcag gaattgcttt tgaaatacca tactcttgaa gcacagcaaa
     6001 aacgcctgca aacgttgtct gtcatgaaag atagtcctta ttttgcccgt atcgacttta
     6061 aagaagaccg tgaaacagaa acattatatc taggtatcgc ttctttaaga gatctggcag
     6121 aagaaacaat cgttattgac tggcgtgcac caattgccaa tctttattat gaaggtgaga
     6181 ttggtcccgc attttatgaa actgaaatag atagaataga tgtcgatctg cagttgaaac
     6241 gacaatttaa aatccaagaa ggtcgtattt tatctatggt agatacgtcg gaagtaatca
     6301 atgatgactt tttgctagag atattggatg aatcatcaag caaccatatg aaaaacatcg
     6361 tctctacgat acaaaaagca caaaatgcaa tcattcggga cacaacaagt aaagtaatgc
     6421 tgattgaagg aatcgccgga agtggaaaga catccgcttt gcttcaacgt attgcttttc
     6481 tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt tcttctattt tcacccaacc
     6541 atttgttttc tgattacatc tcgactgtat tgccttcatt aggtgaaagc ggcgtgccta
     6601 cacagacatt caaaaattat ttggtgcagc tgctgccaga gttttcttta ctagatgaac
     6661 agcaacaaga aagtggtttt ttgctagggg aaaaagatcc gatacaagtg atgaaaagcg
     6721 gattgacctt agtcgatcag attgatcgct atatccaagg catcacaaac tacggtccct
     6781 tgttccgtga tatgaaaata aatggccgta cgatcctatc aaaggaatcg attcgccaat
     6841 ggtataaaga aacaaatgaa caattgcctt tacatcatag actctctcta ttacaaacga
     6901 agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac acgtcagcaa tgggtcaaag
     6961 atctggcgga agaacagctc caagaactat acgcaacaga tccaaatctt gaatatacag
     7021 aacaaaagga gcgttcatta cgtaaaaaac tggccaacca aatcgtacgt aaaagattcc
     7081 gtcgaatcca tcgagggatc atgaattatc aatttgtcaa tataacgaaa caatatctcc
     7141 actttctaca aactgtgcca ctttctcttc taacacgaca taaaatcagc gagaccagat
     7201 ggcaggaaca tttgacaact gttcgtacac acttgcgtca aagagaattg cgtcaagagg
     7261 atgctgtact attcttttta ctgatgcgcc gaacatatcc agtgacggtt acacacaaag
     7321 ctcgctatat ctttattgat gaaatgcagg atttcgcacc atcacaagtg gcattgctgc
     7381 aagccttgtt cccaaaagca aatttgactt tctgtggaga tctaaatcaa aatgtctttg
     7441 gaaatgaaac gatcgttgga tcattggaag accttttcgt ggaccgtgaa gtcactcgtt
     7501 tccaattaac gacaagctat cgctccacaa aagaaatcac tgattttgct aatcatttta
     7561 ttgtgaaaga acatcaagta gaaacaacag caagaaaagg agaccttcct ttgctcatcc
     7621 aaggaacaac agatgatatc aaattgaatt ggttagcaga taccattcga acgactcagg
     7681 gaaaagcccg gtattggcgt acagcaatca tagctaaaac acaagaagaa tgcgaagctc
     7741 tttatgctca actgccagac gagttgaaac aacaagtcca gctgatttta aatgagagtg
     7801 actttatgaa acgttccatt attttacttc cagccttttt agcaaaaggt ctggagtttg
     7861 accgagtttt cttgtgggat gtagatgcag aacatttttc ttctcagcaa gacaaattga
     7921 tcctttacac catgtgtaca cgtgcgatgc atgaattgac cctttttacc tccaataatg
     7981 aaagtccttt gttaaacaat atggatattt catcttatga gaaaaaagta ctgtaagtaa
     8041 aaagacaact gctatcatta atgttagcag ttgtcttttt atttacaata ataattgcag
     8101 aaaaagtaat tcaatcattt aaggtcattt attctatata taaatccaaa tctgtttcag
     8161 gatattttaa tttgacaagc gaaataatca ttttcagttc ttccatgttt tctggatatt
     8221 ctgggtcaaa taccttgcta tgacggccaa tcccgccatt gtcgatcaat cggtaaattc
     8281 gattccattc aatgaattgg tcaattgatt ggaaattgct gattggatag ccgccacaat
     8341 ccaaaatctc tgatacacga aatgctaggt atgcactatc gatcacatta ttttttttcg
     8401 tgtctatctt ctcatttctt tcgataagaa ttttttcagg acgatgcttc aagctccact
     8461 ctaatagtag aaagaaagcc tccatccgat actcttcttc aaataaccga aaggctgaat
     8521 cagaacgacg aaaaagcagc ggaatcagat aattttgtct ataaatcagc attggtgcgc
     8581 ggtccaccat acactctaaa taacggttca gataccctgt accgacatag tttcctaatc
     8641 gatagccttc aacgccgaat cttgcgtatt tttgcgctaa ttttgaacaa tcagttacgt
     8701 tcacagctaa ctcttctact ttgcggtaac cgcttaagat acgttcaaaa ggttctaaag
     8761 tgatttttgc ttccatattc ttccccgtcc atcttccaca tacttcatat catttcacat
     8821 gatacttatg ctctcatcat accacaggtt gaattagaaa agaaaataaa tcacacaaaa
     8881 aagcaagaca atctgttagt gagattgtct tgcttttcga aaaagactga atacttttcg
     8941 ttaaaactgg ttgcttagtc ttctaatgcc acattatgga acacatgctg tacatcttct
     9001 agatcttcca gtgcatcaat cattttttca aatgtttctt gatcttctgg agatagtgtt
     9061 acctcgtttt gagggatcat ttggatctca gcgattgaaa aatcagtgat tcctttttct
     9121 tttaatgctt cttgtatact atggaaatcc gctacttcac cgtaaacgat gatttgtcct
     9181 tcttcttcca ttacatcgcg aacatcgatg tctttttcca ttaggtattc aaggatctcg
     9241 tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt caaacatgta agaaaccgct
     9301 ccggaaacac ccatgttccc gccatttttc ccaaatgctg cccgtacgtc tgctgctgtt
     9361 cgattcacat tgttcgtcaa tgtatcaaca atcaccattg agccgcttgg tccaaatcct
     9421 tcatagcgca attcagagta tgtttcatct cctgcacctt ttgctttttc gattgctcgg
     9481 tcaataatat gttttggtac attataagtt ttagcgcgat caatgacgaa acgtagtttt
     9541 tggtttgcat gcggatcagg atcacctgat tttgctgccg cgtagatttc aatcccaaat
     9601 tttgcataaa tccggctatt attagcatct ttagcagttt tctttgctac gatatttgcc
     9661 catttacgtc ccatattttc acttcgcttt ctgatttgat agtttcataa cgcaaaaacg
     9721 tcctgttcca ttatacttgt gaatcacgat tctgtgaagc tttttttctt tcttccttca
     9781 ttcttctcat ttttagtcaa ttatctatac tcggattggg atttgcccga aattctgttc
     9841 cggaatattg cctcccaatt gatagatttg atcacttcta actaacagtt ccgcttcttg
     9901 tgcctctttt tttccgattc gggaataaat agaaatctct tttttttctt ttaagaatct
     9961 tcttccaaca tcggtaaatc caagtagtcg aagataattt ttattttgtt ggacaggaat
    10021 ctcttgcttt ttgatattca gcaagacata acaagataat cgttggatcc gtgtccatgt
    10081 atatcttttt gtttttacct ttgacacaaa atcctcaaaa ctatcagcag ttgcagctgc
    10141 tgttttcatg cgcgattcta ttccttcggt catctgatag atatctgtca gttccagcaa
    10201 agtagaagat aacaaccgat aacgcaaata cggccaataa gattcccaag tctgcagcga
    10261 ataagttgaa agatcttctt ctgtctgagg cggcacaagt ccagctattc tttttccttc
    10321 ttctaacgca tggcgaatgg ctgttgcact ggctatcctc tgatctgtga tgattttttc
    10381 atgatagcct gcacttttac gtcggatcgg atgtaattcc atagggtgga catacttggc
    10441 attttccttt gcataactta atcccaagat atgatttggc gaagaaaaat caagcgtaat
    10501 atcgggatac agactgcgaa aaacctcagt cattttttgc ggataactat acgtttgatc
    10561 agacagttct tgaaatgcct cttctatcct atcttgattc tcttggacaa agcgtccgaa
    10621 actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa cacaaactgt cacagtccag
    10681 tgtttgcaag atttttactg cacctttagc aaaatagtca gctgactgaa ccgcccattc
    10741 gacgggaagc tccactacta aatccacacc attttgtaaa gcttcattcg cgcgatgcca
    10801 tttatcgatg atagccggtt cgcctcgttg caaaaaattc ccgctcatta cagcaatgac
    10861 gacctctgct cctgttgttt ttcgtgccat ttccacatga tagcgatgtc cattgtgaaa
    10921 aggattgtac tctacgataa tcccgcagct tttcatgcgc atcctccttc atagttatcg
    10981 ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc ggaaaaaata cccgaactat
    11041 ataaactatt ctcgcaaaca caatacttct gcgacgaaaa gctgcactgc aagagcaatg
    11101 cctatatcgt tcggatatat aatttatgtt gaagatttat tcttttcttg ttaactcgag
    11161 caggcaaaac cgctagtctt tgtatgctac aaagaaccag cgctttgatt tctctgatgc
    11221 tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa aagccgctgt tttccagcat
    11281 tcttaaataa ctttccaatg gataggtacg ctcttgatgt agttcatctt cacgaataaa
    11341 tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga aaatgttcga tgctgtgttt
    11401 ctcgcttcct ggataactat cccacaaaaa agcaaaatta tccccttgat aatgatagct
    11461 atattctgga aaaatttcat ctacttgata ggtggagtgt acgtcaaata aaaatcggcc
    11521 attttcttct aacgcttgat agacttcatc aaacacttgc tgtacctctt gtctattttt
    11581 catatagcac agcgaatcag aaaaacaggt aattgcctga tattgtccga tttctgataa
    11641 atcgagcata ttgccttcca caaattggat cgatgcgtct tcttcatatg ctcgctggct
    11701 ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc actttatatc catcttttgc
    11761 aaaggcaact gctaatgctc cagtaccgca tgccagttcc aaaatatttt ttgtgtcttc
    11821 ttgtaaatga cgtttcgaaa aagctagcca ttgctgataa agtgtttcgt ccatcacttc
    11881 atcgtaaatg aaagcaaatg tttcataagc catatgttaa tccaaccatt cactaagatc
    11941 gactagcgga gcatctgacc atagtttttc cagattatag aaacttcgtt ctggtgcatg
    12001 gaaaacatgt acgatcacat ctccaagatc gatcaatacc cattttccgc cttcttttcc
    12061 ttcaatacgt tttacttcat atttgttttc ttcttcttta tcaatgattt cttctgtaat
    12121 tgcgttgatt tgacgttcgc tatttgctga acagatcatg aaataatctg ccaacaagga
    12181 aacatttcga atgtctaaag cgataatgtc ttctgcccgt tttgaatctg ctgcttcaac
    12241 cgcaattttt aattgttctt tactatctat gatgatttcc tcctaatatg agcagcttat
    12301 ttttttgcta cccagtaatt atatgtttcg attgtttttg gataaatctg ttgttcttgc
    12361 gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta cagcttcgtc tagatcagcc
    12421 catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc gacctggttc aatatagtct
    12481 gctacataaa tgattttgtc aaggagactc atcttagcag ccccagtcgt atgcacccgg
    12541 attgcatgaa gaatttctgc atcggtgatt cctaattccc gctctacaaa cgaagcgcca
    12601 acaagaccat gccagattgc attattataa ttcaataact ctggatcaaa accgtctctt
    12661 acgattacca ttttgaactc ctcatctggt cgttcttttg cataatcatg tgtcaatgca
    12721 gcaatactag ctttttcagg agaagcaccg tattttttgg caagagccac ggctgtttct
    12781 tctactccaa gcacatgctt gaagcgttgt tcgctcattc tcatctgtat tttttgcatc
    12841 aggttttctc tagaataagc agtatattca ttactatagt ccatattcat acaacccttc
    12901 attttgaata taatcaatta ctttatctgg aacaaggtag cggatagaac agccttcttt
    12961 gatcttttga cgtattttcg tggaactgat gtcgatctca ggtacatcta cccaaattac
    13021 aggatatgga gtatccgttg tgtatcctgg tcgacgtata cccacaaagt tgaccataga
    13081 agtcagctca tcgattttat accattttgg caaatattct accatatccc caccgataat
    13141 aaagtaataa tctgtatccg gattgttttg cgtcaattcc ttcatagtat cataggtgta
    13201 acttttccca ccacgagcta attcaatcgt ttcaatctgc aaaaaaggat tgtcttccac
    13261 agctaattcc aacatgttga gtcgatgttt tgcatcaatc gtctgtttct catcaacatg
    13321 aggtggctga taagaaggca tcaaaaatac gcggtcaaga cctaaattcc ggccagcttg
    13381 ttcagccatc actaaatgtg caaggtgaac gggattgaat gttccgccca agatccctac
    13441 ttgctttttc ttctctaaga attgaggcat ttcttcagga aataattgtg aacgaacaaa
    13501 tgtcttagct tgcgtattca tcatggtcac tccttatatt gcttttactt cttttgatag
    13561 cctttgatac ttttcttttg tggaaggttt gtataataca ataacacgtc caatgatttg
    13621 gacgatttcg caatctattt ctttttctaa tacttgagct gcttcttctg caatctcatc
    13681 tgtattttga agtaacgata ctttgatcag ttctcttttt tctaaagctt cttcaatttg
    13741 aacgatcatt gctgaattga tcccaccttt tccgatttga aaaatcggct gaagatgatg
    13801 cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa ctcataattt tccttcttcc
    13861 ttaaatcaat gctttgcgtt tcaatacatc tacacccttt ggcgcccagc ccgcaattac
    13921 tactggcgct gttacagtga tccagcctag accggcaaaa acgatgtctg ttttttcttt
    13981 cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg acttcatcag gacgaggagg
    14041 ctgtaataat ccacctacat gtttttgata aaattcgtct gctttttcca gtttcgttcg
    14101 atgaatattt acatcatttg acacataagc aataaatgaa gcacgattgc cgctgataaa
    14161 atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct tcattcagct gataaacttt
    14221 aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct tttttaccta agtagtgcgc
    14281 catttgatgg cgatggataa ttcccggtgt atcaatcaaa aaatgcccgt catctaaagg
    14341 aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa gttgtgatta catcttgtac
    14401 tcccgctgta tttttgatga tttgattgat aagcgtcgat tttccgacat ttgttactcc
    14461 gacgatatag acatcccgtc catcgcggta ttcttcgatt ttttctaata gttccgtcat
    14521 ctcatgagat ttcttagcac tagttaataa tacatctact ggacgaattc ctgcttcgta
    14581 agcacgttcc ttcatccatt gtgttaattt agtttttttc aatgatttag gtaatatatc
    14641 cactttgttc ccaactaaaa gtactggatt gtcaccgaca aaacggtgta gtcctgggat
    14701 tagtgaacca ttgaagtcaa aaatatccac cacattgacg atcaatgcat ctttctgccc
    14761 taattcattc agtaagcgca aaaagtcgtc atccgttaat gagacatctt ggatctcatt
    14821 ataatggcgt aatctgaaac aacgctgaca atagacttcc ccggaagcta aacctttttc
    14881 caaagccgtt tgcggcgtgt atcctaattc accgggtttg tttgtttgta taacggcacc
    14941 acagccgata cattgtaatt cttctgtcat tcgattccac ctcgccagcc catatcaggg
    15001 tgttttgcta atagataggc cataattctt ttttcccaaa aacgattaaa ttgtgtcttc
    15061 caactatctg ttgcgataat gggttttaca aggatgctac gtatccctgc acgattggct
    15121 ccacgtatat cagtcataat ctgatcgcct accattacta tctcatcatc tgctaaatcc
    15181 aattgtttct ttgctatgtt gatccctctt gctaaaggtt tcattgctcg tgcaacatac
    15241 aaaagatcaa atttttcaat cgctcgcgcg acacgttttg aattattgtt tgaaacaaca
    15301 acaacaggga ttcctgcatt tttcatttct aaaatccaag taagcaactc ttctgttcca
    15361 tctggattat tccatgcaat caacgtattg tctaaatcgg tcaatactgc tttgatgcct
    15421 agattcttta attgggcagg tgtaatctga tagatggctt ctaccatcca tgttggtttg
    15481 taatttgaaa acatgctgtc tcctcatctg caaaaaatga aggagcacat aaagtactcc
    15541 tcaaccccgt attatactgc atatcagcaa aaaaaactac ctctatactc gttcgtcttt
    15601 cagaaaaagc atttctttac aaaatgacca aaaataaaga ttgatcacag aaaaatttag
    15661 aaacaaaaaa caactaagcg agcaagacca ttctttactc tttgcttagt tgttaaacac
    15721 ctaatattgg cggaacctct tgtatcgttt ttccagtaat tcatgctggt cgaatcgaac
    15781 aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt tatatcgtgg
    15841 caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta actcgcgtag
    15901 ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac cgtctttcca
    15961 caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt ccagcatcca
    16021 gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac caatgatgat
    16081 ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa tagcctctcc
    16141 ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga tcaatgtaat
    16201 caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc gatagccttc
    16261 aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc ctttttgaat
    16321 accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa tcgcttcgtc
    16381 atctgcaaac aaacgatcac catggcattc ctcgaaagaa tcgaagatct cttcaaaaaa
    16441 atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat gtgctgtctt
    16501 atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcatatgg attttctgct
    16561 tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag aattcagcct
    16621 tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt cttccagcaa
    16681 aaccgatcaa gctttggggt tctgctagaa tgatatcgcc ttccattgca aaactggcag
    16741 ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct tcattgctat
    16801 gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt ccttcttgca
    16861 tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt gttgctcgtt
    16921 cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg ataaaattag
    16981 gatccatcac tccaataatg aagggaatcc cttgaatggt tgctttacca gtgaggactg
    17041 cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat tcgggaaatg
    17101 ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt tcatcaataa
    17161 tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga gggcatactt
    17221 tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac tttgcccaca
    17281 tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga ttgatccgaa
    17341 tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt tctggtgtcc
    17401 aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct atgactgcag
    17461 ggtgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct gtgataaatt
    17521 cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca tggacgatta
    17581 tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa actgcactgt
    17641 ctacccgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc cctggagaag
    17701 gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg tgtccttgga
    17761 atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc acctgttttt
    17821 ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg attcgagtat
    17881 tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc gttccggcat
    17941 tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct tctctttttg
    18001 tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattatta cgttgcaaag
    18061 aacaatctcg ttctcctaaa tgaatgacat tgccaaaatg atcacctaaa atctgaactt
    18121 ctatatggcg agtaggataa atgatttttt ccaaatacat atcatcattt ccaaatgccg
    18181 ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt gattgaactt
    18241 tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga taaccaattc
    18301 tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt cctggaatga
    18361 ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc attgcatcga
    18421 tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg gaggcaaatc
    18481 ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt gtcacgattg
    18541 cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc gggccaatgc
    18601 agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct gaatatatcg
    18661 ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg atttcaccgc
    18721 ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa ggttaattcc
    18781 gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc ggctgaagct
    18841 ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac tttcttacgg
    18901 aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg ttcaagagaa
    18961 agtaatgcaa ctgctcctgc ttgagccatc gcttcaacaa ttaatacccc tggcataaca
    19021 ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt tttcttagca
    19081 acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat tggataacga
    19141 tgtggaatga tttcttttat ttcttgtatg ttcattgatt tccctctttc ctgctttgct
    19201 tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc gttttctaca
    19261 aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag tttcatcgct
    19321 tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt gaaagctggt
    19381 ttgtcgggcg ccggttgcag ataaacgatt cccacgattg gcgaaacaac ctcgattcca
    19441 tcaaatactg ccgattcttt ttctttcatt gtttctggag cagttgttgc ttgttcaggt
    19501 atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat attcatgcct
    19561 cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa atcgaattcc
    19621 gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat atccaaaatt
    19681 attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct aatgaattac
    19741 tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc aagtcaatct
    19801 caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct aatgccagca
    19861 aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc atcgatttcg
    19921 tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt gcttttgctt
    19981 ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg acttgttccg
    20041 gctgtacacc agcttcttcc atcgcaagct tcattgcttt agacgcacca gaaccatctg
    20101 gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaatg atttctccat
    20161 agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga acacctgctc
    20221 cttcacccat cacaaaacca tttcgatttt cgtcaaacgg aatcgaacca cgattaggat
    20281 cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc tcacaaatcg
    20341 ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg ttacggaatg
    20401 cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta caaatacctt
    20461 ttgcacctac gcgcaaagca atatttccag ctgccatgtt ggcaatcgcc attggtacga
    20521 acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg ttttcgattg
    20581 ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga ttcatttttt
    20641 ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca tagatcgaaa
    20701 acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg aaatctttga
    20761 cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc ggtgcgatgc
    20821 cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca ataggtgaag
    20881 tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt tcttatccgt
    20941 gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga ttttgtgcta
    21001 agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc ggaatttgct
    21061 tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt gtgatgaatc
    21121 ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc acagattttg
    21181 ttaatcctac tacgccagct ttactagctg cataatttgc ctgaccaata tttccaatca
    21241 acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc atttttttca
    21301 atacgtgctg cgtcatattg aaggttccaa tcaaattgat atcaagacat tttttgaaat
    21361 cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg tttactaaca
    21421 catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct ttctcataat
    21481 cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt tcttctatct
    21541 tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct tttgcaaaag
    21601 ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca ttttttcctt
    21661 ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg tctgcttatt
    21721 ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc ttaatacttt
    21781 tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat ggatactctc
    21841 atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg tttctttctt
    21901 cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga attgaatctg
    21961 acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat gaaaaggccc
    22021 gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta aaacagcttc
    22081 gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat aatttgcagg
    22141 agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt caatcggcgc
    22201 attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca tataagcgcc
    22261 tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag ctactaatgc
    22321 cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat gctccttcaa
    22381 taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt gcgtctgatc
    22441 caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa tctcacttgc
    22501 ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca taccttgata
    22561 ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca atatatccag
    22621 aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc aggaaatgtc
    22681 ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa gtcactaaat
    22741 cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca tcttcaattt
    22801 cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg atgtcagtag
    22861 tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct tgaatttttt
    22921 cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta taatttaaca
    22981 gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc tgtaaatatt
    23041 actttctgct gttttcccaa ggtgatcttt ccactagaaa ctgcttcatc caataagata
    23101 gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa cttttcttgc
    23161 ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg atgcaaaagc
    23221 agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac gtctcgtacg
    23281 gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc tgaatcttct
    23341 gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact tctggtaccg
    23401 tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa gagaatccca
    23461 cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat tagcttagat
    23521 agtgtttcac caccaatcac tagtccctta gttgagccgc agcggatcat tttttcagcc
    23581 atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc gatagcatga
    23641 gttgctccca atttcccttg tacttgtgct gctacagatg gcgtcgtgta atcgggcgac
    23701 attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg ttttatcaat
    23761 ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca tcttcgttca
    23821 cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt ggaaagatca
    23881 ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat tccaaattgt
    23941 tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt cagtgcatcc
    24001 agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac catttcacga
    24061 tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc caactttaca
    24121 acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac taacttgtta
    24181 atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc tgaagttgtt
    24241 tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga cagatctttg
    24301 aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa gacactaaca
    24361 agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact aattagtttg
    24421 attatcaaat attttgatgt tcaaactata tcttaacttt tttcaaaatg caaccttaaa
    24481 agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa acaactgata
    24541 aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat tttgattatc
    24601 aaagtatttt ctgtgaacag aactatagaa ttccttaaaa cttgtccatt atttcaaaat
    24661 ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag aaaaaatgaa
    24721 aactctcccc atgctgccgg gtgtctatct tatgaaagac cgtgatggta aggtcatcta
    24781 tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc agaataaaca
    24841 gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt tcgtcgtagt
    24901 agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt accgtccttt
    24961 ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta ccaataaggg
    25021 atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc tctataaaaa
    25081 aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt tgtctgaaat
    25141 tagtttgtta gctttgcgag cccagcttcc tgacctacag gaaatgactt ttgaacagaa
    25201 aaaaaaggaa ctccaagggc tgtttcaagg ccaaaataaa aagctactaa cttacttgaa
    25261 aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta tgcttcaaaa
    25321 agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc attttttaag
    25381 aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga aaaagcacta
    25441 cctcgtttgt tatggtcaac ttgcagaaac aatgattgcc tctgctgacg tttctcctga
    25501 cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt caaaagaaga
    25561 aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag aacagtctga
    25621 aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc ttctcggctt
    25681 cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga ttaagtcata
    25741 gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag tatctgtttc
    25801 agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct ctacgccttc
    25861 tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag cctccagtcg
    25921 ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc ttatttctgt
    25981 tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa ctcgtttttt
    26041 atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa gcttgggttc
    26101 cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct tgggtagttg
    26161 ctggcaatac aaggtccatt ttttctgctt cttctaatgc aatctttaaa tctttgataa
    26221 aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga gaataattgc
    26281 ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc tttaaatcaa
    26341 gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta cctgcaatca
    26401 tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc aatgtaaatg
    26461 tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct tctccaccga
    26521 ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc ggtgcatcta
    26581 acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg cttggtgtac
    26641 ttgtagtcaa gtcaactaaa atatgtcctg agatatctgc agaaaaaatc cccgactgtc
    26701 catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga atattacttt
    26761 gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact aaaggatcag
    26821 cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt agatggcgtg
    26881 cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt tcgacctcct
    26941 aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat tttttcataa
    27001 aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg ttcaatcgag
    27061 tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc ttgataaaat
    27121 tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc ttctaaaact
    27181 tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc aaagtcttcg
    27241 tcggtttcaa aagttttccc taatgaagga tgatcaaaca atcggttcaa ataagcattt
    27301 ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag cccttgtgtt
    27361 tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc tacatcattg
    27421 ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga gatcaattcc
    27481 tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt aggataagcg
    27541 gagatgacct gaggaaaccc attaatcaag gcttggatat ttttttcata agaaatcctg
    27601 cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct taatttgctg
    27661 atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc agtaggaatc
    27721 acgcgaagcg gtgctattac accatagcta cgtaatgtat caataacacg ttcacttgga
    27781 caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac atgggattgc
    27841 ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt atggtaagta
    27901 tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc tgctccgaat
    27961 tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata agcataccac
    28021 atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact tggcattcga
    28081 atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat atagacttca
    28141 tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac tccgctgact
    28201 tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc caaactagtc
    28261 cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta cactttcgta
    28321 tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg tttgataacc
    28381 ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc ggataaattc
    28441 ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat tatacactgg
    28501 aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa cgatcccttc
    28561 tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac cttctatgat
    28621 ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt ttacaatttt
    28681 ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa atgtgtattc
    28741 gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga aaaacaaacc
    28801 gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga acttttgttc
    28861 tttcattgga tcagggcgat atttctctac gtcgatccca ttggaaatag cttgaatcgg
    28921 cacacggata ctatagcttt ccaacaactg ttttgaataa ggtgttggcg tgatcagata
    28981 gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa gctggttcga
    29041 tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga caactttttt
    29101 tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg tattgatgtg
    29161 caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt ccgtcagagc
    29221 acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct tttcactttc
    29281 aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata acatagccta
    29341 caaactgtca ctcaaactta agccctattt ttatctattc ttactgacaa aaacgttttc
    29401 ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc ttttgatacg
    29461 ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcgta tcgcattctt
    29521 tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc attaaactac
    29581 gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata cctactagca
    29641 atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat tttccttcag
    29701 cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta tatggttggt
    29761 acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga atcaaatcgt
    29821 tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag actgctgctg
    29881 ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg ccttcactga
    29941 tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct ttgagtgtag
    30001 ctaccatagg gaacatgata cgtaagttac catgaacaga tgcacgaagc aatgcacgca
    30061 tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat gcacgatatc
    30121 ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta tctccaccga
    30181 tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca gctttgtaag
    30241 cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg aattcagtac
    30301 ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct tttggtgttc
    30361 cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct gcatttttta
    30421 atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca aattcagctt
    30481 tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca gccaaaatat
    30541 cgccttcttt gactttagca gtaatttctt ttgtaccaac gattgctgga atttctaaag
    30601 aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca aacgctttta
    30661 caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca acaacgacca
    30721 cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc gctaaaatac
    30781 gtttagcaac atcacgaata tctgcagcac gctcttgcat ataagcatta tcttccattg
    30841 cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca gcatttacgc
    30901 tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa agaaccatca
    30961 aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct ttttcacgaa
    31021 tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct tcttcagccg
    31081 cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc aggtaagctt
    31141 ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc attattcaga
    31201 taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt cgtcagcacc
    31261 ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa cgcccatgat
    31321 tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt taaatttgct
    31381 tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg ctactacgtg
    31441 aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt ttatgtgagg
    31501 gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat ctgtaaagat
    31561 taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa agtattcaaa
    31621 ttattccgga ttctttttca tccttttctt cacattttat aacaaaagtg tttattttct
    31681 ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac cctctttgtt
    31741 ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag agtcaaaggt
    31801 caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa aaaactaggg
    31861 gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca tttttgtgaa
    31921 aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa cgattcactt
    31981 gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct gttatcaaaa
    32041 aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc catttggatt
    32101 tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc aaggaaatta
    32161 tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg gtcagccacc
    32221 tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag aacaagcacg
    32281 acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag tcattgaaat
    32341 tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg ttggtaaaac
    32401 agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc aaaaattaat
    32461 ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg gtatacgagg
    32521 acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag aaaatgtgat
    32581 cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg atggaaatat
    32641 ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa tggttggtgc
    32701 tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac gtcgtatgca
    32761 acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga aaggtttgca
    32821 aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg aagcagccgc
    32881 aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca tcgatttgct
    32941 agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta aaacaatcga
    33001 gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag aagatttcga
    33061 aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag aacgtcagct
    33121 cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg ttgaacaacg
    33181 gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga aaaacttagc
    33241 agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag tagcgaaagc
    33301 gattcgtcga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct ccttcttatt
    33361 tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct acgaactatt
    33421 cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa aacatagtgt
    33481 ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg gtcaattaac
    33541 agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag aaaaagctca
    33601 tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga cagatgcaca
    33661 aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg ccggaactgg
    33721 taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc gttctgtatt
    33781 gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg ggatcatcga
    33841 attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc tagacgatgt
    33901 caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg tcaaagaaaa
    33961 attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac gcacgatcca
    34021 agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta tccatgagtt
    34081 gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag aacgtttggc
    34141 taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga gacggtattt
    34201 ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt cattttataa
    34261 aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg tgagcgccat
    34321 gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc ttgaaaacac
    34381 tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct acactgaagc
    34441 ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc cgaaagaaat
    34501 cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc acgaggatga
    34561 aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac atgaattagg
    34621 cgctacatta gccgtgtaaa caaattttga acataataat taaagagcgt gggacaaaag
    34681 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc
    34741 cttcggaaat aagctaaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
    34801 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
    34861 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
    34921 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
    34981 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
    35041 tttcacgtat ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
    35101 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
    35161 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
    35221 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
    35281 taattttaca ttaggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
    35341 gaacaatggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
    35401 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
    35461 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
    35521 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
    35581 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
    35641 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
    35701 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
    35761 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
    35821 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
    35881 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
    35941 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
    36001 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
    36061 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
    36121 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
    36181 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
    36241 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
    36301 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
    36361 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
    36421 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
    36481 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
    36541 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
    36601 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
    36661 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
    36721 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
    36781 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attctggatt
    36841 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
    36901 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
    36961 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
    37021 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
    37081 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca tcgaatgaaa
    37141 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
    37201 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
    37261 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
    37321 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
    37381 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
    37441 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
    37501 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
    37561 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
    37621 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
    37681 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
    37741 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aaaccgaatt
    37801 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
    37861 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
    37921 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
    37981 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
    38041 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
    38101 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
    38161 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
    38221 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
    38281 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
    38341 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
    38401 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
    38461 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
    38521 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
    38581 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
    38641 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
    38701 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
    38761 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
    38821 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
    38881 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
    38941 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
    39001 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
    39061 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
    39121 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
    39181 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
    39241 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
    39301 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
    39361 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
    39421 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
    39481 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
    39541 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
    39601 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
    39661 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
    39721 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
    39781 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
    39841 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
    39901 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
    39961 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
    40021 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
    40081 tttttgtagt cgaacagggt atgcctttgt ctcgagaaat agacaaagat gaagcccatt
    40141 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
    40201 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat ccttttgaag atgctggcat
    40261 gcctcatatt tatatgaaaa agactttata aaaatttatt tcgaaaaaag caaaaaacag
    40321 caagcttttg agaaaaatct ctcactggct tactgttctt ttttgtgttt acttacgcga
    40381 caattgcttg taacggttat accaaatatc aatatagcct tccgagaacg ggccatctcc
    40441 attattgatc cagtcaacca gaatcttcac attttctttt aggatgaagt caatatcgtt
    40501 cggataattc atcattttgc tgtgcatctc atattcatcg acatccaata gtcgtttctc
    40561 gccgtcagga aataccttga tatccaagtc atagtcgata tatttcaagg cctcatcgtc
    40621 cagcaaaaaa ggtgaagcta gattacaata ataggaaacc cccttttctc tgatcattgc
    40681 tattacattg aaccagtatt ttttatgaaa ataaacaatc gctggttcgc gcgttaccca
    40741 tcggcgtcca tcagattcag tgactaatgt gtgatcattc acgccgatca tagagtgttc
    40801 gcttgttttt aataccatgg tatctcgcca cgttcgatgc aatttgccgt cgtgcttgta
    40861 actttggatc gtcacaaact ctccttcttt tggaactccc atagcaaccc ttccttaagc
    40921 agcattttgt gctttgtgtt tgggtgttca ttacaccgac atcataatta ttatatcaat
    40981 aaaaatgagc tttgcctaga aaggattcct attgtttcga tttttctcta actgatctac
    41041 cagtttcatt tggaccttag gaaaaacgac cgaatcaaat gcatcaggct tcaaccactt
    41101 cgaagttttg ttctcggtaa attcttgttc tgctccctcg gttgcccgtc cgtaaaaaag
    41161 gagaacatgc catttcagat gactaaaaat atgagtaact tctcctaaat gtcgtgtctg
    41221 ccaaacaaag agttcctgct tttcaaatgg gagattttgc tcttcttctg ctactaggtc
    41281 atcaaacaga tcgatttctg gctttgtttc ccattctttt ttcaatcgct catactcctc
    41341 ttgtgtcact tccatcattg gaaatgtcca catattcgcc agcaattttt ggctatctct
    41401 ttcttcaaaa taatacgctc cagaatgatt ttgaagagcc gcactgatat aataaacgtc
    41461 ttttggcttt gctttttttg tttttacagg aaaactcgtt tggattcctc gtttgttagc
    41521 taaacaaaag gcttggatcg gacaagtctc acactttgga gaagttggtg tacagatagc
    41581 tgaacctaga tccatcattg cttggttgaa ttcacctgga tgcttttcat caatgatttt
    41641 ccgcatcgct tcatcaaaga ttttcctgct agaagctttt gcgatgtctg cttcaatgca
    41701 aaacaatctg ctgactactc gcatcacgtt gccgtcaacc gcgggttctg gaagtccaaa
    41761 tgcgatactt gcaatcgcgc ctgtcgtata tggtcctatt cccttcaatg agctaatttc
    41821 ttcaggcgtt tgcggcattt ccccatcaaa ttctgacatg atctgtttag cagcagcttg
    41881 gatgttccgg gctcttgagt aataaccaag gccctcccat gcttttaaaa gcttttcttc
    41941 tggagcattt gctagctctt caattgtagg aaaccattcc atgaaacgat aaaaataatc
    42001 gatcactgtg tctactctgg tttgctgaag catgatctca gagatccaga tacgataagg
    42061 atcacgatta tagcgccaag gcaggtttct tttttcttgt tcataccatt gaatgaactg
    42121 atcttgaaat tcttttgttt ctttatctgt ccatttttcc cattctttat tcactttcct
    42181 cttccttttc cgtaatgaat cgctggatca tatccaaatc aaatcctttt tgatataagc
    42241 tttgctttac ttttagattt ctttttttag gttcaaacct taaatttctt cgccatagtt
    42301 tatctccttg tttgactaaa taatcatatt ctatttcttg atctgcttct tgcggaagat
    42361 cagccatcgc ttgttggatc acttcttgag aaaaaccttt cgtcatcaat gtttgttgga
    42421 tttttctcaa taattctttt tggctttttc cgcgattttt tccaaacgct ttttcggctg
    42481 tctgatgtgc attttgaact tggtcttcaa atgtatattg gctgagcgct tgttcaatga
    42541 tttcaggttt caaacctttt tgctgcatct gctgcgctaa tttctttggt cctttgtcac
    42601 ttaagcgcat attcgtgcgc acataacttt ccccatagac caaatcatcg acaaggtcca
    42661 attcttttaa gcgggctacg attttatttc gatcttcttg aaggatttcc ttttctttta
    42721 agtatgttcg gatttctttt tccgatcgca actgataact taagtaattc attgctaggt
    42781 gaaaaccgaa atcagctccg ctactcttct tgatctcttg gatagtttct tcgtctaatt
    42841 cccgtccttt taataatcga tagcgtacta atacatcttc agatacttgt aaagcttctc
    42901 cttcagataa atctacgcga tagaaagggc ctttgccttt actgattctc gtaacggtta
    42961 acataaacat cctcctcgaa catatattct ctatcatacc acagaaaatc cccgtaatca
    43021 ggtattatat gttagaataa accacagaaa aaagaaaaag ggagatactt atggcagaaa
    43081 caattgtcaa aactggacaa acggttcctt tgaaaatcaa gcgtttaggc ataaatggtg
    43141 aaggaatcgg ttatttcaaa cggttgatca tatttgttcc atatgcacta ccaaaagaag
    43201 aagtactagt gaaaatcacg aaagcaactc ctcgctatgc agaaggagaa ttaataaaag
    43261 tcaaaaaaac aagcaaagat cgcgtagtgg caccatgtcc tgtttactat gaatgcggtg
    43321 gctgtcaatt acaacactta gcttatcaag ctcaattgga ttttaaaaag gatctgcttt
    43381 tgcaagcatt ggaaaaattc aaaccagcag gctttcgatc ttatcaactt ttgccgacga
    43441 tcgggatgga tgagccttgg cattatagaa acaaagcaca atttcagtta cgaaaaaata
    43501 agcagactca aaaaattgaa gctggtttat acgaagcaaa ctctcatcag ttagtcccta
    43561 taacagactg cttggttcaa gaacctcgaa cacaaaaagt aatgaatact gttgttcaac
    43621 tattgaataa atttgatgca cccatctatg atgaacggac aaacagtgga atcttccgca
    43681 caatcatggt tagaatcggt gttaaaactg gggagttaca agttgttttt attactcgca
    43741 gtaaaaaatt cccgcaaaaa aacgcaatga tctgtgagat caatgaacga ctgcccgaag
    43801 ttgtttcaat catgcaaaat gtccaggcac aaaaaacgtc tctcgtgatg ggggatgaaa
    43861 cgatccactt atggggaaaa gaagccattg aagaacaaat caatgaagtg actttcgatc
    43921 tttctcctcg tgccttcttt caactcaatc ctcagcagac cgaattgctt tatggcgaag
    43981 gaatcaaagc attagatgtc cagcctaatg aaacgatcgt ggatgcttat tgcggagttg
    44041 ggacgatcgg cttaagtgta gcaaaacaag ccaaagaagt acggggaatg gataccgttc
    44101 ctcaagcaat cgctgatgca aaaaaaaatg cggaaagatt aggatatcac aacactcgat
    44161 atgaagtcgg gacagcagag caagttctgc ctaaatggtt gaatgaaggt tttcagcctg
    44221 atggtatcat cgtagatcca ccacgtacgg gtttggataa cgcattgatc caatcattat
    44281 tgaaataccc tccaaaaaaa cttgtttata tctcctgtaa tgtttctaca cttgccagag
    44341 atctggttaa tttaagtaaa aagttcaaga ttgaatattt acagtctgtc gacatgtttc
    44401 ctcagactgc acgttgtgaa gttgtcgtga agatgacgag actcgctgac taaatgaaag
    44461 aggttgtgaa aaagtctgcc ctgtatcaag taataagaac agccaaacaa aaatagctct
    44521 tcatgtttta tgaataccgt acttgtgacc gaagagttaa caggaggaca ctatttattt
    44581 aaaaaaaggg ttgtgacata gtttttgtca cagccctttt tgattattat tggatattca
    44641 cgtctatcac ttgataaaat gcatttgtcg tatcgctgat attccatact gctaaaatca
    44701 cattatatcc ttttcgatcc tgcggaagtg tgatcgtttg gttgactgtt tttggtggaa
    44761 cagttgcttt gtcgtctatt tttgtgatca attcaaaatc actaaatttc aaaggttgat
    44821 taggattcca agttggtttg gtaatgtagt aatcccaagt acttgtccga tgttgtgctg
    44881 ttaaattcca agtaatattt aatgttcctg gattaaccat gtttttatgc catctgttag
    44941 atgtctgttc atctaaaggc tcaaagccgc tgactcctgc actagcaatt tttccatcaa
    45001 tgaatgtatt tttggtagct tcgatacttt gcggttccca ttgtgctctt ccgacatttt
    45061 gatttaagtt tcctgctgct gatgttccta ggtatgcacg gcttcctgga gaagtaatat
    45121 aaccatgcgc aaaacctgga atgctaaaac cgaacaaccc taaactaact aacccaataa
    45181 acaataacgt actaatcttt ttcatgtaga aacctccttc atttgataac cacattgtaa
    45241 acgctttatt gtattaacac aaacattatg cttttataat tttatgttga agtaaaaaag
    45301 gtgaaaaaag taagatgcag attataacag aagtagacga gaaaagtaca aatagaaaac
    45361 atgagaacca aatcatatga tctttttcaa aaacatcatt catataaaca aatgttcaac
    45421 tttttaataa caacaatcat caattttttt cttacttcac aaactaaata caggtcttgc
    45481 tgtgagcctt catggtaaac tactattata gaaaggagtt agtaaaatga agatgataaa
    45541 agatattcgt ttttgggttt gtgttatagg aattgtcata ttaggctttt tatcagggtt
    45601 gcttagcgga aatcccggag aatattacta ttctttgcaa cttccaccat ttgcaccacc
    45661 tagctggata tttgggccga tgtggacctt actgtatata ttgatgggta tttctcttta
    45721 tttgttactt aatcacaaca acaaaaaaca gcgaaacaat cttgtcggtc tttttgtgat
    45781 tcagttcatt tttaatttta tatggtctgc tctatttttt aatttaagaa atattttcat
    45841 tgcagcaatc gatattacac ttttagttat tttcttgtca gtgcttatgt atcagttgtg
    45901 gctacatcat cgcctggcaa tgtggctcat gattccttat tatttatggg tactattcgc
    45961 aactctattg aattattcaa tttatttttt aaactaagcg ctaaaaagag acgtcctgta
    46021 atttaacaca tagaaaaagt gccacctaag ttagattttt tgtctaattt aggtggcact
    46081 ctttattaac caaactattt ttttctagta cccacttgtt gttccatact cattattctg
    46141 gtcataacca taaccatttt gttcctgtcc agcgtaagga tcataacttt gagtatcttg
    46201 acttggtgta tagtactgac ttgtatcttc tgtttgctga taataggtat tcggatcttg
    46261 gtactgatca tatgtgtttt ctgaatagga agaataatcg gtgtaccctt cactattcga
    46321 tccatcaccg tttgtcgaat cgccatcttc cgaatcatca aagaacatca ttgaagcatt
    46381 ctcatttttc aaattaggta atgtatcgct tgtttttata tttaattgtt tcttcagttc
    46441 attttggatc gaaagaagat cattttttcc taaaatttga tagtagatat cattgatcat
    46501 ttgtccttcg ccttgcaact ggtcttgttt gattgtttca aacgcagggg tgtagttcgt
    46561 cgctacatca agcatgtcat cccaatctaa gtccgtcgtc acattgttct caactgcttt
    46621 taaaattttt ctataattgc ttacgccatc taaacttaaa actttattga cgattttcgt
    46681 cactacttcc cgctgacgac gttgtcgacc gatatcgcct tctggatctt cgtgtcgcat
    46741 tcgagaatag gctaaacctt tttctccatt caatgtttgt tcacctactg gtacatggac
    46801 accatccaaa gtaaagtcaa ttttattatt cactttgatg ccaccgaccg cattgatcaa
    46861 atcttccatg ccatccaaat tgatcgttac ataatgatca ataggaatat cgagcagttg
    46921 ctcaattgaa tccatggcca tttcgacacc accaaatgca taggcatgat ttaatttatc
    46981 taccgttcca taacctacga tattcgtata aatatctcgg tccaaactga tgatcgttga
    47041 ttttttttgt ttcggattaa ctgttactac catcatcgta tcagaacgtc cttgttccgt
    47101 tcttcctaat ccaccggtat ctaaacctaa tagcaaaacc gaaaaaggtt ctttgtcatc
    47161 aatgcttact ctctcagaac gtttggagga tattcgatta gagtttttag aaattttgtt
    47221 tactgtttgg ctcgcttctc ccataaatct gattccgtat gctgataatc cggcaatcag
    47281 caaaacaaat acccctaaca aggttaatag aactttttgc catcgcttca taatacacct
    47341 ctgtttcaca ttttctaaaa tattgtctca tataactgtt caatcagcaa attcactttt
    47401 taatcataag aaaactttaa aaaacatttt tgtgtaacga ttactatttt ttaaaatttc
    47461 tcaaaattct taactatcat accataccgt gaaaaagatc gaaagaccga atacacgcag
    47521 ttcgcaatcg ttttatcttt ttttacgtat tttttccaga aaaagcgaac aacaaggtat
    47581 tttccaaata atcgtgcagt aaaatgatcg cacatacctt tcccatcttt ttaacagtct
    47641 ttgcctaccg ttcagattat cattcgattt cacgaaaatt tcttcctatg actggtcctt
    47701 ttgttaaacc catcttctat tcgttttatt tccagctcac tgcatggctc ttaaaatgac
    47761 tcgaactagt gatttcatta ccatcatctt ttaatggata gatatgctcc tcttcaaagt
    47821 aaaacgattt tcttttaaag taatagaatt gacatggtaa tgagtatgta tactagtggt
    47881 ttcacaaatg gtcttttatt caacatccaa aaactgaaat ttctttaaaa tccattacta
    47941 acgaagaaaa tgttccttgc ttgataatta gttaggacat gtcaagtgaa caaatgcatt
    48001 tacttagcca aaaaatctaa aaacttcata aatagacaaa aagacacgac tctctaagaa
    48061 gtcgtgtctt tattcaacaa attattataa tttagtcact tcagcagctt gaggtccgcg
    48121 tgcaccttca acaatcgtga attctacttc ttgaccttct tctaggctct tgaagccgtc
    48181 tccttgaatt gctgagaaat gaacaaatac gtcatctccg ccgtctactg taataaaccc
    48241 aaatcctttt tcattgttga accatttaac cgttccgtgt tccataaatg tatagcctcc
    48301 aaaaaagttc ttgcaagtaa ccttgcaaga acattatacc caatttagaa aacgctagca
    48361 agctttcgct cttttatttt ccaaattatt tttaagactg atatgacgga ggaaatggtt
    48421 attttgctgc ttcgaaacgt ttgtttactt catcccaatt aataacattc cagaatgctg
    48481 caatataatc tggtcggaca tttttatatt tcaaataata agcatgttcc caaacgtcca
    48541 aacctagaat tggtgtcttt ccttccatca atggagaatc ttgatttgca gtagaggtaa
    48601 tagctaattt tccattttcc attacaagcc aagcccatcc agaaccaaat cgtccagcgg
    48661 ctgctttttt gaattcttct ttaaaagaag aaaaatcacc aaaagcttca ttaatcgctt
    48721 cttttatttc tcctgtaggt tcgccaccag catttggtgc catgatttcc cagaaaaatg
    48781 aatggttagc atgtccgcca ccattgttac gtacagctgt cttgatatct gttggaatag
    48841 cgtccatatc agataataat tcttctattg ttttttcgcc taattctggg tatttctcaa
    48901 ttgctgcatt taaattcgtc acataagtat tgtgatgctt atcatgatgc agatgcatcg
    48961 tttcttcgtc aatataaggt tccaatgcgt catatgcata aggtagatct ggtaatgtgt
    49021 aagtcataaa aaaattcctc ctcaatgttt tctttataat gtctacatta caaagagtaa
    49081 caaagagaag gaaattcttc aattgttatg cctagtcgtc tttttcactt tgacctactt
    49141 cttcttcaaa aacctcagtt tctttaaaaa taaacaactt gctgaaaata tagttcgcta
    49201 ggataacaat aatttgagtg atgattttcg ccacatactc attgctgttc ataacattga
    49261 tcaacaatac cattgccccc atgtccaatc cgtaagaaag gatacggtag aagaagaact
    49321 taccgcattc aataagtgca tgtgtaaaat tcggtgattt tgaatggaat acccaaagtt
    49381 tgttcgttac aaaagcaaac aatacagaac aaaaccacga aatagaattg gataaggcca
    49441 tacttaaatc aaaatattgg cgtgcgagag caaaactaac aaaatttaca atcgtagcca
    49501 aaccaccaaa aaagagatag actagtatct cccaatatcc ttttgcttct aaatatcctt
    49561 ttaactgtcg atactgcttc atgagccaac ctaccttatt aaaaattctt atttatcata
    49621 ctctatattt caataacttt catctttatt taaaaaaatt aatttttttt gatcctttgc
    49681 ctttgtctct gcttggatcg tcacatgtgt aataccatat tttaatttta ataaagaaat
    49741 tatacgagga tatatctttt caatctcgct tagtagctga tcttcaaaat caatatgtcc
    49801 gtcgaaaata attatctttt cgctatactg ccaaatatgg acatgatgaa cattctttac
    49861 cccatcgatt gcttcaattt cttgttccat tttttcatag tcaatagcag gtgcactttg
    49921 tatcaaaata gcggcagctt ttttgatcac cattatcgct tctatcaata tatatatcga
    49981 aatgatcagt gtaataactg gatcgatcca gtagatatcg aaaaaacgga tcaaaattgc
    50041 tccgataatc acacttaccg aagacagtgt atcacttaac aaatgcaaat aactagattt
    50101 tatattcaaa ctttcatctg cttcttgtcg taataataat actgagaaca aattcgcgag
    50161 caatccaata actgctactg ttagcatcaa atgactattt atcttttctg gtgaagtgaa
    50221 ccgcttgaat gcttcaacgg ttaaaaacaa ggataatatg atcaatatga atgagttgat
    50281 gaaagcactc aatatttcta aacgctggta gccatacgta cgttttttat ttgcttcttt
    50341 tcgggccatt ttttgtgccc cgtaagaaaa aataattgcc aatgtatcac taaggttatg
    50401 gaaagcatct gaaacaagag acaaactccc tgacataatc cctccaataa attctgataa
    50461 agtgatcacc gcattcaaaa ccactactcc aaatagtttt tttgatgatc tctgtggctg
    50521 tgtaggcact attccttcct ccattttatt tatattggat agtctttaga caagaataac
    50581 atttttttga tatttcacca atcattcaga tgtttcccta aagcacttgt atttcacttg
    50641 atacttcgct agaattgcaa gtgatgacac tactagatga ggagtactta tgacaacaaa
    50701 acaattaatt atcagttcct ttatacgatt caaagaccta aaggaggcga gaaatgtccc
    50761 attttggaaa gccattgtct atcttttagt tttgagtata attatggctt tgccaatctc
    50821 ttttcaagtt tttcaagtct taaataacat tcaattggat gggcaaaaga ttgctactcg
    50881 cattcctgat tttacaatcc aaaacgggca gcttcaaacc gaagaaaaag aaggattcat
    50941 ttaccaaaca aactccatta tcttcacatt tgatccagag ggaaaacgaa cagaacagga
    51001 catctctact gatttgatgg ggaattttgt tagtgtcggg atgttaaaaa acaaacttat
    51061 catagctctt cctaatacag gcacgacttc tgctttgttg aacaataacc aattagaact
    51121 tccctacacc aatgaatctt taaaaaactt aacaggtaag cagctccgtt cttttttaag
    51181 tgaagcatct ataccacttt ggataaaagt cctgacattt atattttcta tttatcctag
    51241 ttttctgaat ctgctgatca ctttgctatt taccaacatt gctgcttttc tctatgcacg
    51301 ctttcgacta acaaaagcta cttttcttga ttgtttaaaa acattgatct attcaatttc
    51361 attgcctact ataattgcaa caatcttgat ggtctttctt ccttcttttg acactagcgc
    51421 cttcattgct tttgcaggaa tctttatttt tgctcaagct gtcaaaggat gggcaaaaat
    51481 atcgatcaat taagaaataa agacaactcg ttatttctac ttaaatagac ccttttagag
    51541 gttgtgacat ttttttgtca cagccccttt tttatttgaa aaatatttct ttctcaaaaa
    51601 acaagcaagg cgcttcaaag ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg
    51661 gaaattaact atgtcagaaa gaatcattta tgcagatcgt ccatttcgtg atgaatttat
    51721 cgaagaaatc catactatag cgcctggtta tatctttaaa acagaattac aaccagagga
    51781 tctacaaaat gtagagatca gtttgggctg gaactcagac taccaagaaa aactattagc
    51841 ctcttctacc ttgaaatggg tccaatctat ctcagctggt gttgatcagc taccccttga
    51901 gactttttca aagaaacata ttttactttc aaatggtagt ggcatccaca gtgaatcgat
    51961 tacggagcat gtcttaggaa tcatccttgg gtattctcgt ggtctttttc aagcacaaaa
    52021 ggcacagagt gacatgaaat ggttaggttc atcggtccat taccaatcta tccaagggaa
    52081 aaaaatacta atcattggaa ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc
    52141 attagacatg aactgctatg gaatcaatac atccggtcat ccagcagaag gaatggaaaa
    52201 gactttttct ttaaatcaac taaaggaagt tcttcctgaa gtagatttcg tagtcaatat
    52261 cttacctcta acggaggaaa caactgggct ctttgatcag catatgtttg aacactttga
    52321 tccaaaatct gtattcatca atgttggccg tggtgcttct gttaagacac aagatctggt
    52381 gcaggcttta aataaccagc aactttcctt tgcagcatta gacgtttttg aggaagagcc
    52441 tcttccacaa gatcatcctc tttggaaaat ggacaatgta ctgattactt cccacattgc
    52501 aggtttgaca cctgattttc aaaagaaatt aatggctatt tttttaacca atctgaaaag
    52561 ctactttgcc acccatgagc ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata
    52621 gctgaaaaca atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc
    52681 tttttttatt gttttttaaa gtgataggtt ggctcctcca ggttgggatc ataatcaaca
    52741 agtaaatcat atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca
    52801 tccatttcca ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa
    52861 ccatcatgaa cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct
    52921 ggcagatcaa tttctgagtc aaaccatttt tgtgcttttg gcgttaccgt taatttcatc
    52981 ccaacaacct actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca
    53041 tcgcctgtct ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca
    53101 ggagaagcga taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt
    53161 tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt
    53221 tctgctaaag aatatttatc tgacagctct aagtagtcta gaatattctt tgatactaag
    53281 ttatgtgcaa tccgtatccc catgtctcgc tcagggtgta cgacacgatc tgctccgatc
    53341 ttctctagta cacgggcatg gtactcattt tgggcttttg ctaaaatatt cggtacgccc
    53401 atttccttta ccatcagagt aactaagatg ctggcttgga tatcttctcc aatagcgaca
    53461 attacatgat cgaaatttct tatacctaat gaacgaagtg tcatctcatc ctgtgcattt
    53521 gccacaacag catgggtggc tatattcata tattcattga ctcgatcttc attactgtca
    53581 atagccagta cttcttgtcc agcttcgatc agtgtacggc agatgcttcc gccaaatcgt
    53641 ccaaggccta caattgcaaa attttgtttc accattttcc tcctttagta tcttcttttc
    53701 tcagtataac aaagcctttc caaaaacaaa agcaagcatc attattgtaa aaaagccaag
    53761 aaaaataata gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac
    53821 caaaccatgt ttaaacgcat agatagctgc ttgagttcgg tcttctacct ctaacttagc
    53881 taatatattc gacacatgcg ttttgactgt tttcagtgtg ataaacaatt catccgcgat
    53941 ttcctgatta cttttccctt cagaaatcag catcaaaatt tcattttctc gattcgttaa
    54001 ttcttcatgc aatactggat catttcgccg actcattttt tccatcattt ttgttgtcac
    54061 ttcaggttct agtactcgct cgccgcgttg cgtagccctt atagcatcag ctatttcatg
    54121 tgctgtagac gtcttcaaca aatatcctgc tgctcctgct tcaattgcag gataaacttt
    54181 ttcatcatca ataaaacttg tgacgataat gattttggct tttggccaat cttttaagat
    54241 tgcttttgtt gattcgatgc catccatttc ttccatgact agatccatta aaatgacatc
    54301 cggtcgaagt gccatcgcct tttcatagcc ttgacgtcca ttttcagctt ctccaatcac
    54361 ttcaatgtct tcttggatag ataaataaga agagacgcct aagcgtacca tttcatggtc
    54421 atctactaat aaaactttta tcatctttat ctatgcctcc ttcatcaaag ggaccttggt
    54481 ctccacgctc gtaccttgcc ctttaaaact aatgattttt actgttccac caataccagc
    54541 tactcgttct ttgatattgt tcaatccata acttcccgtt ttggtttcat tcatattaaa
    54601 tcctgtcccg tcgtcaatga tacgtaataa aagattgtta tctattttat gcaaatagac
    54661 ttctaattca ttagccttag catgtcttaa tgtgtttgat aacaattctt gaacgattcg
    54721 gaacaagtga tcctcaatgg aactagttaa ttttacatct tctacctccc attttagcga
    54781 aatctggatt ttattctgca attccttcaa taactgctca atgccttgtt ttaaactttt
    54841 ttcttctaaa ttgactggac gcagatgaag aagcagcgca cgcatctcag actgggaagc
    54901 gttgatgatc tctgccacca ttttcaactg cttacggaac atttctggtg tctcactttt
    54961 ttctgcttgc tccgtcaatg ccgacatcat catcatcgct gcaaataatt gctgtgaaac
    55021 tgaatcatga agctcacgag ctaatcgatg cctttctaat tttaaaatct cttctttggt
    55081 ttgtccatct acatattgag gacggctagt cataatctgt aattcactgg atacttcgat
    55141 cattttttct tttattttag taatttccat atcaatatct ttgatgtaca gatcatccga
    55201 cgcgcttggg atcggctgat gcaaaagttt ggattcataa ttgccagaag aaagtgcacg
    55261 aagcttttct tcgattttcc cgtattttgc cttttgaata agtgataaca atagaaatac
    55321 gatcaatcct acacctaatg aaattgccat caaatggaag ataagcggaa cgtataataa
    55381 acgcgcacgc agcaattctc cccaccaatg actttggtta ctggcataaa aataagtgaa
    55441 aagtgaaaat agaataataa ggaaagccgc aatccccgaa taaacagcta gcattgcttt
    55501 ggatattctt cccatcatat tcgtatcacc tcgacatctc caaccaaagt attggtaatg
    55561 atcttcaatc tgcgaggatt ttcatcataa tcactgctgc agattttgat ctgttcgttt
    55621 tttaaggaaa actgctcttc ttcaaacaag acatttccta ctaatgtcgc atgctctaaa
    55681 cgaatagcca ctcccagggg tactaagatc cgtgttctgc ctatcccttt tcttacaatc
    55741 acaatattat catcttttgg caaaagtgta ttgcccaaat cgatgatagt atcaccagaa
    55801 atgatggcaa tgttgatatc atcccattca tagacgtcgt ttccaatacg ttggttacca
    55861 aataattgct gtcttttacg ctcattatta tgtgttttta attgctctgt ttggaccatc
    55921 atgatctgtt tttttcgcca aaaagcattt tttgtcaaat ctattcctga aatctctaca
    55981 cctttcagcc cgataaataa aacggccaat acaaccatca tccataaagc aggactgttg
    56041 actaagctaa aaaaaatgac caagctccct aagatcaatt gaaaattttg aaatttagta
    56101 cgaggatatt tgcgcatagc tagatatata ttgaaaatac cgaatattac taaaagcaaa
    56161 agtccagtat tgtttacgat ttgccaaaca gcaaataaca gcagcagtgc ttcgactacc
    56221 acaaaaaaac gccaagaact attcatcgct ttttctccct cctgacacat tctcatttca
    56281 tcatacctat ttttttgact gttggctata ggactttaga tgtaagatcg ttcagtgaaa
    56341 cctgcaatac tcaaagagaa aagatctaga gtgcgtcatc agtaagatga actctctaga
    56401 tctttcagtc agactacgct tgttctactt tgacgatttt aactaacata tcgccacctg
    56461 gtgtagcaat ggttacttca tcatttttac gtttgccaat caatgcttgc gcgataggag
    56521 aatcattgga aatctttcca gaaaacgggt ctgcttcagc acttccgaca atcgtgtact
    56581 cttcttcatc tccatcaggc aactcgatga acgtaactgt tttaccgatc gatacctcat
    56641 ctgcatctac gccgtcatta tcgatgattt gtgcaaaacg gatcatattt tcaattgttg
    56701 tgattcttcc ttcaacaaat gcttgttcat ctttagctga ttcgtattcg gagttttcgg
    56761 aaagatctcc gaaaccgcga gcgattttga ttcgttcaat gatttctccg cgtttaactg
    56821 tttttagttc ttctaattct ttttctaatt tttctttacc ttctagagtc attggataaa
    56881 ctttctctac catgtgtaac gtctcctttt ttcgataaaa taccgcaaaa ggtaatcttt
    56941 tgaggaatat agtccaaaaa agattaccac gcttttctta aaatgtaaat gtttttctaa
    57001 aaatgatcaa tgactagccc ctgtttttct aagaattatt tacatatttt tgcactagtt
    57061 ccatatgttc ttcatacgtt ttagcgaaat aaacatttcc agtgctcgta tctgctacga
    57121 aataataata gtcattttct gctggttcta atactgcctt gattgcctct tcgcttggat
    57181 tgtcaaaagg accaggccca tagccggtat tggtatacaa attgtatggt gaatctacag
    57241 ctgtatcttc atacgtcacc aattctttat gttctcctaa tgcatataaa acagagatat
    57301 ctgactgaag tggcatattt gccttgattc ggttaaagaa gacttgtgca atatttttac
    57361 gatcattttc ttttacgcct tctttttcaa ctagagaagc caaagttaaa acttcttgta
    57421 cagtcaaatt tttctgtttg atagctgaat aatagttttg catgaccgta tttgttttgt
    57481 tcaccatctc aatcacaaga tccttaagcg aagtactcgt gtagtaatca taagtagctg
    57541 ggaataagta gccttccaaa cggtattttg tgttctcagc tttagaggca ctttccaaca
    57601 attctgggaa tttttgatac aactcgttaa agaaagtctc atcatttact aaatctaaaa
    57661 attctttctt gtctttacca gttaccttcg ctacccgctc agcaatctga tcgatgtcgt
    57721 agccttctgg aatggcaatt ttcccatctg caacttttgt tggctcagaa gtgccgcctt
    57781 cttgcagctg tttgccaatc tcgtcaagag tcatattagg cgctaactga taatagccag
    57841 cttgaaagcc agttaaattt ttaaattttg tataataatt gaaaacaatc ccgcttttaa
    57901 taatattgtc cttctccaaa atctcaccaa tctgcttgtt agaagagcca ctcgggatct
    57961 ccacttggac taattggtca tctgatttat ctaaaggctt caacccgcta tctacataac
    58021 gatacacggt aaatccaaga aatccgccaa taattaataa tgtaagagca atcactaaaa
    58081 tgatcttcct cacaatctta tcttcttttt tacgcagatt tctccgcttg tcgattatat
    58141 ctgctgtagc tgccttactg gcttcttttt tatctttact tgggtgttgt ttgtttcctg
    58201 acaaaacaac tcctccttac tatcatcttc gtaactctgt ttattataca tgaaatttct
    58261 actgccgtga attattttac caaatattat gtcaaaattg taagtggcaa atactctttt
    58321 tgaggattac aatgaaaaat agttaaaaat ggatagaaag gaattaacat gtcaaaagga
    58381 aaacatcgaa aaaaacaaaa aatttctatt tcttttcttg taattttatt attgtctgtc
    58441 agcttctttt caacaggcat cattatagga ggtcaattaa caaaaatacc tcattcccaa
    58501 acttataaag cggagccaac acaacttact tccaatttaa cgcccgatga gcagaggctt
    58561 gctagcaaaa ttgatcaatt actacaaaaa tcagattata tcggttcgat ttatgttcgc
    58621 aaaaataatc gcgtactatt agaaaaagga tatggatatg caaatctgcg cgtaaaagaa
    58681 tcgaattcac cttctattta ctatcaaatc ggttcgattc aaaaagcaat gactgcttta
    58741 ctgatactaa aacaaatcca agctggtaag ttaacttttg acacaaagtt agcagaattt
    58801 tatcctcaga ttcctggaag tcagcaaatc acaatcaaaa atatgcttta tatgcgctct
    58861 ggtcttcatc gaactgcttc tcccgacaaa cctatgtcgg atagtgaaat cattcagttc
    58921 gcccttcatc atttgaaact tactgattat gatacgtatc attatgaacc attgaattat
    58981 acattgctta cagggatatt gatcaaacta acgaatcagc cttatgaaac tcttctaaaa
    59041 aaagaaatca ttcgtccact ccatctagaa catacagatt tttatgacaa tgtaaaaaat
    59101 tcgcctcaac atgctgtatc ttaccagatg tctgcaactg acgattattc aaaagcattg
    59161 atcgagcctg aaaccgacat ccgaaatgaa ttaggtacag gaaacataag tatgtctgtt
    59221 tatgacttga acaaattctt taccagtgtt ctttctggcg gtatcatacc aaaagaacta
    59281 cttttctcat tgtggcaaaa taatggtgat aaacatcctt acagcggcgg tgtttacagt
    59341 ggaaacgatt atatccttgc tcaggggaat atcaatcgat tccatgcagt agtggcgatg
    59401 aaaaaagaca ctaaagatgc aatcgtcatg gaaagtaata tccaatcgga taagaacatc
    59461 aaattgccgg ccactgattt aagaaatcag atttatgaac tgatggaagg tgttcactta
    59521 tcaaattaaa ttttgacata atattagttg tcatctctgt atagtcaatt tagttagtac
    59581 tctatctgaa aagtagaaat aaattaacaa atattatctg tccttattat ttgttaattt
    59641 atttcttatt tta
//
LOCUS       DAZKCZ010000005        59388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000005 DAZKCZ010000000
VERSION     DAZKCZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.005"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..495)
                     /locus_tag="ACG8KN_000235"
     CDS             complement(97..495)
                     /locus_tag="ACG8KN_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9187326.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(492..1265)
                     /locus_tag="ACG8KN_000236"
     CDS             complement(492..1265)
                     /locus_tag="ACG8KN_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9187327.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1262..1945)
                     /locus_tag="ACG8KN_000237"
     CDS             complement(1262..1945)
                     /locus_tag="ACG8KN_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9187328.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2112..2999)
                     /locus_tag="ACG8KN_000238"
     CDS             complement(2112..2999)
                     /locus_tag="ACG8KN_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9187329.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(2996..3865)
                     /locus_tag="ACG8KN_000239"
     CDS             complement(2996..3865)
                     /locus_tag="ACG8KN_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9187330.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4002..5288)
                     /locus_tag="ACG8KN_000240"
     CDS             complement(4002..5288)
                     /locus_tag="ACG8KN_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9187331.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5561..6139
                     /locus_tag="ACG8KN_000241"
     CDS             5561..6139
                     /locus_tag="ACG8KN_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9187332.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6139..7068
                     /locus_tag="ACG8KN_000242"
     CDS             6139..7068
                     /locus_tag="ACG8KN_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9187333.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7065..8801
                     /locus_tag="ACG8KN_000243"
     CDS             7065..8801
                     /locus_tag="ACG8KN_000243"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9187334.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8864..9700)
                     /gene="pheA"
                     /locus_tag="ACG8KN_000244"
     CDS             complement(8864..9700)
                     /gene="pheA"
                     /locus_tag="ACG8KN_000244"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFP9187335.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9697..10206)
                     /locus_tag="ACG8KN_000245"
     CDS             complement(9697..10206)
                     /locus_tag="ACG8KN_000245"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFP9187336.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10199..11494)
                     /gene="aroA"
                     /locus_tag="ACG8KN_000246"
     CDS             complement(10199..11494)
                     /gene="aroA"
                     /locus_tag="ACG8KN_000246"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9187337.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMSADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11507..12598)
                     /locus_tag="ACG8KN_000247"
     CDS             complement(11507..12598)
                     /locus_tag="ACG8KN_000247"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFP9187338.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12615..13781)
                     /gene="aroC"
                     /locus_tag="ACG8KN_000248"
     CDS             complement(12615..13781)
                     /gene="aroC"
                     /locus_tag="ACG8KN_000248"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9187339.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13783..14847)
                     /gene="aroB"
                     /locus_tag="ACG8KN_000249"
     CDS             complement(13783..14847)
                     /gene="aroB"
                     /locus_tag="ACG8KN_000249"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9187340.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14880..15902)
                     /gene="aroF"
                     /locus_tag="ACG8KN_000250"
     CDS             complement(14880..15902)
                     /gene="aroF"
                     /locus_tag="ACG8KN_000250"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFP9187341.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16561..17535)
                     /locus_tag="ACG8KN_000251"
     CDS             complement(16561..17535)
                     /locus_tag="ACG8KN_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFP9187342.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17516..19399)
                     /locus_tag="ACG8KN_000252"
     CDS             complement(17516..19399)
                     /locus_tag="ACG8KN_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFP9187343.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDIK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19417..21642)
                     /locus_tag="ACG8KN_000253"
     CDS             complement(19417..21642)
                     /locus_tag="ACG8KN_000253"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9187344.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21757..22791
                     /locus_tag="ACG8KN_000254"
     CDS             21757..22791
                     /locus_tag="ACG8KN_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9187345.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22858..23871)
                     /locus_tag="ACG8KN_000255"
     CDS             complement(22858..23871)
                     /locus_tag="ACG8KN_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9187346.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(23949..24137)
                     /locus_tag="ACG8KN_000256"
     CDS             complement(23949..24137)
                     /locus_tag="ACG8KN_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9187347.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24577..24705)
                     /locus_tag="ACG8KN_000257"
     CDS             complement(24577..24705)
                     /locus_tag="ACG8KN_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9187348.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24708..26081)
                     /gene="rlmD"
                     /locus_tag="ACG8KN_000258"
     CDS             complement(24708..26081)
                     /gene="rlmD"
                     /locus_tag="ACG8KN_000258"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9187349.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26192..27247)
                     /locus_tag="ACG8KN_000259"
     CDS             complement(26192..27247)
                     /locus_tag="ACG8KN_000259"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9187350.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27257..28687)
                     /gene="gatB"
                     /locus_tag="ACG8KN_000260"
     CDS             complement(27257..28687)
                     /gene="gatB"
                     /locus_tag="ACG8KN_000260"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFP9187351.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28684..30156)
                     /gene="gatA"
                     /locus_tag="ACG8KN_000261"
     CDS             complement(28684..30156)
                     /gene="gatA"
                     /locus_tag="ACG8KN_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFP9187352.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30156..30461)
                     /gene="gatC"
                     /locus_tag="ACG8KN_000262"
     CDS             complement(30156..30461)
                     /gene="gatC"
                     /locus_tag="ACG8KN_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFP9187353.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30567..32603)
                     /gene="ligA"
                     /locus_tag="ACG8KN_000263"
     CDS             complement(30567..32603)
                     /gene="ligA"
                     /locus_tag="ACG8KN_000263"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9187354.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32620..34854)
                     /gene="pcrA"
                     /locus_tag="ACG8KN_000264"
     CDS             complement(32620..34854)
                     /gene="pcrA"
                     /locus_tag="ACG8KN_000264"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFP9187355.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35009..36568
                     /locus_tag="ACG8KN_000265"
     CDS             35009..36568
                     /locus_tag="ACG8KN_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFP9187356.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36679..38610)
                     /locus_tag="ACG8KN_000266"
     CDS             complement(36679..38610)
                     /locus_tag="ACG8KN_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFP9187357.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38628..39542)
                     /gene="pfkB"
                     /locus_tag="ACG8KN_000267"
     CDS             complement(38628..39542)
                     /gene="pfkB"
                     /locus_tag="ACG8KN_000267"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9187358.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39539..40291)
                     /locus_tag="ACG8KN_000268"
     CDS             complement(39539..40291)
                     /locus_tag="ACG8KN_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9187359.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40451..40660)
                     /locus_tag="ACG8KN_000269"
     CDS             complement(40451..40660)
                     /locus_tag="ACG8KN_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187360.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40671..41999)
                     /locus_tag="ACG8KN_000270"
     CDS             complement(40671..41999)
                     /locus_tag="ACG8KN_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187361.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42135..42290
                     /locus_tag="ACG8KN_000271"
     CDS             42135..42290
                     /locus_tag="ACG8KN_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFP9187362.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(42364..42951)
                     /gene="yihA"
                     /locus_tag="ACG8KN_000272"
     CDS             complement(42364..42951)
                     /gene="yihA"
                     /locus_tag="ACG8KN_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9187363.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43034..44284)
                     /gene="clpX"
                     /locus_tag="ACG8KN_000273"
     CDS             complement(43034..44284)
                     /gene="clpX"
                     /locus_tag="ACG8KN_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFP9187364.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44531..45820)
                     /gene="tig"
                     /locus_tag="ACG8KN_000274"
     CDS             complement(44531..45820)
                     /gene="tig"
                     /locus_tag="ACG8KN_000274"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9187365.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(45974..46906)
                     /locus_tag="ACG8KN_000275"
     CDS             complement(45974..46906)
                     /locus_tag="ACG8KN_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187366.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(47063..47722)
                     /locus_tag="ACG8KN_000276"
     CDS             complement(47063..47722)
                     /locus_tag="ACG8KN_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP9187367.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47725..48750)
                     /gene="sppA"
                     /locus_tag="ACG8KN_000277"
     CDS             complement(47725..48750)
                     /gene="sppA"
                     /locus_tag="ACG8KN_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9187368.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEKGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48763..49053)
                     /locus_tag="ACG8KN_000278"
     CDS             complement(48763..49053)
                     /locus_tag="ACG8KN_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187369.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49462..51267
                     /gene="glmS"
                     /locus_tag="ACG8KN_000279"
     CDS             49462..51267
                     /gene="glmS"
                     /locus_tag="ACG8KN_000279"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9187370.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINCLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(51339..52694)
                     /gene="glmM"
                     /locus_tag="ACG8KN_000280"
     CDS             complement(51339..52694)
                     /gene="glmM"
                     /locus_tag="ACG8KN_000280"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9187371.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(52718..53887)
                     /locus_tag="ACG8KN_000281"
     CDS             complement(52718..53887)
                     /locus_tag="ACG8KN_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFP9187372.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(53884..54771)
                     /gene="cdaA"
                     /locus_tag="ACG8KN_000282"
     CDS             complement(53884..54771)
                     /gene="cdaA"
                     /locus_tag="ACG8KN_000282"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFP9187373.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(54960..58652)
                     /gene="nifJ"
                     /locus_tag="ACG8KN_000283"
     CDS             complement(54960..58652)
                     /gene="nifJ"
                     /locus_tag="ACG8KN_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9187374.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            58851..59372
                     /locus_tag="ACG8KN_000284"
     CDS             58851..59372
                     /locus_tag="ACG8KN_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFP9187375.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        16438 a        12901 c        10673 g        19376 t
ORIGIN      
        1 atttcataag tattcacgag aaaaatacaa acaaaaaagc ggaaataacg ctcatccgcc
       61 ttcttgtttg tgtttatttc ctcttcaaac gcttgtctat gattcagact tttctctatc
      121 gttattttca tctaaaagtt cttgcattcc gataatttct cgatattgat gaatcttagt
      181 gataccaatg actttgtcta catcaatact aaaacaaata ccattacctg gaatatcgat
      241 gttgatgcct tgaccaatcg catccattac tttattcgtc aaactatcgg gtactaatgt
      301 caacacaata tctttttctg gcgaaatgtc caaacctaaa aatttccctg aatcatgtac
      361 accagaacct cttccatgta agatcgttcc gcctgctgca cctgcttctt ttgaaaagtc
      421 gataacatct tcgcccattc ctccgtcaac gatcgtcaca atcatgtgaa gattcaatgc
      481 tacaagatct ttcatttgct ttcccccaat ttttgcaatc tttctttttc gattctgtct
      541 ttttcatctt ttcgtttata aatatatcct agtactaaag tggataatat tggtgtcaat
      601 gcaatcattg caaccacacc aaacccgtca atgatcggat ctcttccttc aatttgtgca
      661 gcgattgcca cgctcattga taaaataaat gtcgaacaca tcgggcccgt tgctactcca
      721 ccgttatcaa aagccattgc taaaaagatc ttgtccactt ttcgtcctaa aataaatgct
      781 agcccgtatc ctgggatcaa gaaataataa agagagaatc cgacgagcaa acgccacata
      841 gacaatccta ctgctgcgcc gatcccaatt gaaattacca tcagcatgat tttcgatcgg
      901 attctcccct cgctcaattc ttcaacctgt ttgatcatta catgaatcgc cggctcagca
      961 aaccctacta agaaccccat taaaaagcca aggggaacca gtatataatt gtaaggcaat
     1021 cccgcaatag cactgcctaa atgctgtcct accggtacat aagccatatt gactccgtgc
     1081 aggaaaagga tcaacccaat agtcgttatc aagaaacctt tcataatgtc aaagaaacgc
     1141 tttttgcgca tacgaaaact aatgatattc attaaaacaa ataggattac gataggtgcc
     1201 actgccaaaa tgacttccat ggtaacgatt tcgattccat ctatcacttg ctgccagaaa
     1261 ctcattggaa gatcaccccc aaaatcatca cggcaagaat cggccctaac gaagcaatac
     1321 cgaccattcc aaaactgtca ttttcatttt tcttttgttt gatcatacta gtcatccctc
     1381 cggcaagggc caagataaat ggaacagtta ctggaccagt agtcactccc cccgaatcaa
     1441 aagcaatcgg cataaattcg gaactggtga aaaatgaggc gatcaaaacg cctatgtagc
     1501 ctatagccat caattgataa taggatagct tgaatacgac ccgaagcagc gcaaaggcta
     1561 gaaatactcc agtgccgacg ctgacgatcc caatcagcaa cactctgcca atctctccgt
     1621 cagatatatc atatacttgt tgtcccaaaa cttgcacgga aggttcagca atcgtgatca
     1681 cgatcccgat agcaaatccc aaactgacca aaacggtcaa acttttcttt ttgatcatat
     1741 atttccctac tagatctccg acttccatca tagaataatc tgctccaaac aaaaagagcg
     1801 tcattccgat catcatgatt gccgcaccaa ccagaaatga agcgaggtct tctccttcta
     1861 acggtgcaaa aacaaacgtt aaaatgacaa tcaggacagt catcggcaat atagcagcaa
     1921 tcacttcttt aaaatttttt agcatgtatc ctcctccttt actctttaag tttaccttaa
     1981 aatgaaagag ttttcgagca caaacttaaa acgttatcat ttaatttttc tcaacataaa
     2041 ataacacacg tcccaaaagt atctttttac ttggaaaaat gactttgaag gaacgcgctt
     2101 attgtgtttt tttattttac agagccgatc attccttgaa cgaattgctt ttgcaaaaca
     2161 aaaaatacga tggcagttgg taaagtagcg atgactaatc cagccatgat cattccatag
     2221 tctggtgtat aggaagcacc catagcagat aaaacaagcg gaaccgtccg tttatcagga
     2281 gattgcaaag ctactaaggg ccataaataa ttgttccagc tactcatgaa tgtgatgatt
     2341 gcagccgcgg cataggtatt tttagccgtc ggcatataca ctctaaaaaa aatacttagt
     2401 tcttttagac cgtctaatcg agcggcttct actagatctt ttggaaaaga tttaacattt
     2461 tgtcgaaaga agaagatcaa aaaagcagtg ctaacagatg gcaggatgac aaccaagaaa
     2521 ctgttgattc ccaaaaccgt tccgttcaat tgtgaaaaca ttcgatataa agggatcatc
     2581 aatgccgcaa aaggaaccat catcgatagc agtagaatat tgaaaattag gtcttttcgt
     2641 ttactttgat aaatctcaaa tccatatcct gccattgacg aaatcagcaa tgcaaacaca
     2701 gtggtcacca aagcgatcat cgctgaattc caaagggaac gagtaaaatt cagctcattg
     2761 gaaaacaaat tttgaaaatt gatgatcaag ttactgccaa tctttatttt tcccgatgtg
     2821 atatccacgg gtgtattcgt cattcccaaa accatccaga caaaagggaa aatggaaata
     2881 aatgatagga tgcttaatgc tgtatacttt aatactgacc caattgtcga acgaagttct
     2941 aatgcttgtt ttttgacgtt ggggcgttcg attgtttctt tttctaccat atttttcatc
     3001 gactttctcc tcctactttc gtttggatta tcgacaatat cacaataaag gaaacaataa
     3061 caaaggaaac tgctgccgca tatccaaaat taggcgtgaa tttataactc aaattataga
     3121 tgtattgaga tagggtggtc gttgcatttg ctggtccccc gccagtaata ttttgtactt
     3181 catcaaaaag ctgcaatgtc ccgatcgtcg aagtaatgga agtaaacaaa atgattggtt
     3241 ttaaatttgg gatcgtcact ttgataaact gctgccattt agaagcaccg tcaatagatg
     3301 ctgcttcata aatctccgaa tcaatatttt gcatgcctga aaggaaaaaa atcatattgt
     3361 atccagtcca acgccaagtg atcgaaagaa tgatcagaac tctcgcccaa attggatgag
     3421 tcaaccagcc aatcggtgtg tcaatcatac caatgcccaa caacaagttg tttacaaggc
     3481 cagattgaga aaatagactt ttcatgatca gagaataact gaccatcgaa gtgatacatg
     3541 gaagaaagat agcggttcga aacaaccctt tatatttcaa ctttttatca tttaaaaggt
     3601 tggaaataaa aagtgctaga aacaacatga tcggtacttg tacaagtaaa tacaggaatg
     3661 tattaaaaaa agctttttta aaagttgcat ctgtcaacat ccgctgataa ttacctaacc
     3721 cgttaaatgt catattgaca gggggaccac tttgaaaaga cgtgatcaaa gccaaaacca
     3781 tcggaataaa aaccatgatt gatatcaaaa tgactggcaa gattaaaaaa gcatgcccgt
     3841 ttttgaataa atctttctgc ttcattcttt tccctccaat tctatcattg attatctaca
     3901 tgaaaagaca agaaagacaa tatctgtaag gcagacgtga tcttttttgt cttttcgttt
     3961 gtctgatgaa aaagcgaaga aattcacttt tttaccaccg atcaattagc caattgtgct
     4021 tctacttgtt tttgtgtatc tgctagtacc ttgtctgtct cttctccatt gatgattcga
     4081 tgaagagctt cagctacgac tgcttcaatt gcataggtct catgtccgta attcacttct
     4141 ggtatttcag atgtccatgt tgaaaaatcg tcaaagatct tttgaccact gtaaaattca
     4201 gaagcttctt gataagcttc ttgttcattc gcgctaagca tcgtagatac tagtccgatt
     4261 tctttcgcaa gagtgcccat cagttcttca ttcgaagcaa acgttgcttc taggaaatct
     4321 ttcgctgaat cttctccagc tactcctttg atcacatacc agccagcgcc gccaaggtta
     4381 gaagcttgcg ctttttgcag attttctgga agtgcaggta ttggtgcaat cttccatttt
     4441 ccagattggt tttctgctcc ttggatcgtt gaagaatacc aagcgccagt tgggctagaa
     4501 gctactgctc ctgattgtat cgcagttact ccagcattcc agtcagaggt ttgttcgact
     4561 agatcttctt tcaaaagagt ggcaaacaat tcaagaccat actttaaaga tgcattgtct
     4621 tggatcgtga ctgtctcccc atcttcagat gtataccatt ctcctgcctg ttgcataatc
     4681 atccttactc gaccaaggtc agaaggattg acttcagcga ttttctttcc cgtcttttct
     4741 ttgacatctc gcgctacttg aatatactcg tcccacttca gattctccat atcctcttct
     4801 gtataacctg cttctgccat cagatccgta cgatagaagt ttgctgttac gcccgaatcg
     4861 aaaggaacac cgtatatttt atctcctact ttgtttaccg caaatttata cggtgcaaaa
     4921 tgatcttcct ttacaatagc agacaaatta gcgaatgcat ctggataaga agtcaaatag
     4981 ccttgtatcc tataatcttc aatcaagacg atattgggca atccttctgt attcccgctc
     5041 gctaaagcgg tgttcagttt ctgaacgata tcatcctgag acatcgtaac aacttctatc
     5101 tctgtttctt cgttctcata aacttttttt gcttcattca ctgctttgat attgaatgtc
     5161 tcatcccacg cccagacagt gactttttct tctttgtctg ctgtactact agcacttccg
     5221 catcctgtta agaaacctga tgctgccgct aaacaaacga ctccagcaat taactttctg
     5281 atgttcattt ttatccgctc cttttccatc cattagttgt ttacgtttgc tacattcatc
     5341 ataccaaaat ttgaaaacgg tttctttatt attcagatac ttcaaaaatt tctcctaaaa
     5401 taataaaaaa atacaaatca actatgacaa aaggaataat cttagcatct agaccaaaga
     5461 tcgaaatggt gaggtttctt taaaataaaa atatccttgc attttctcct agaaataagt
     5521 atttttaaat tacaagacta ttcttaaggc gggtaaaaaa atgccaatac gcgtaattct
     5581 tatcgatgat gaacagacga ttttagatgg aatgaaacag ttgatcaatt ggtctgagca
     5641 cgggtttcaa ttaataggga ctttttccag tccgctgaaa gcgcttgatt tttctttggt
     5701 acatgcaatc gatatcgtgg tcacagatgt aatgatgcct gaattaaatg gaatcgaatt
     5761 atcgcggcta ttaaaacagc aacaacctga aacacagatc cttatcctct ctagctatga
     5821 tgaatttgat atggttaaag attcttttaa agaaggcgtc gctgattatc ttttaaagcc
     5881 tcgattgagt cctgattttt ttctaaattc cttaactgcc gtttcacaaa aaatcaaaaa
     5941 aaagcccaac aaaagcccaa tcaccaatcg ttatgagcag atctgcgaga atctttcaca
     6001 gcagattgct ggacaaactc gtccattgct tgaccctctt ctttttgaac acaccttgtt
     6061 tttctgttat ataccgaaaa acggccattc agttcaaaac aaggctctaa acaaaaacag
     6121 tcgatcaccc attcattgat ggatagcgac gattcgttta ctatttatcc tgtttctacc
     6181 ttttcggaag aatccggcta tgtaatcaat actgattcca aagatacact taggaagttt
     6241 gttcaaaagt ttcgtcaaga agtttttcca ggtgattttt ttgttgtttc tgattctttt
     6301 gcaatccaag atatcaatga aatattccaa gagatcagaa attgcaccaa aggacaacgt
     6361 ttctatcata agggaaagaa ccttgcttat cagcatgaac ttcttccgtt attaaccaac
     6421 cacgagcagc aagcaagtag ttatttaaca aaaatcgttc ataaagacta cgctttagct
     6481 gtcaaagaac ttgagaattt cttcacgacg atattggaac agcctattga tccagcttat
     6541 ttgaaacatg aggcaatcaa tcgtttctat gcattactca acgctttggc tgacgagcat
     6601 gttgggaatg aagaactttc tttgctcaag atcgcccttc cttctttatt agccgatgct
     6661 gagtatttcg aagatttttc tattcttgtc aaagaaacac ttgagaaact cacccacttt
     6721 tctgcaaatg caaaaaaaga caatttcgat atcttgactt tgatctacga gttcgtccag
     6781 gaaaattatg agaaagaaat caatttacaa cttttatccg aaaaatatca tttttcatat
     6841 agctatctct cagccatctt tactgaaaaa tttggtatca acttttcaaa atatctaaaa
     6901 aaagtccgta tcagtaaagc caagctcttt ttgacggaaa cacggcttac tttgacggaa
     6961 atctgtgaga aagtcggcta taccgaactg ggttatttct ccagagtttt taaagaagag
     7021 acaggaatga caccttctca ataccgtaaa ggaacttttg taaaatgaga aaaaaaatca
     7081 aagagcatga actctttgcc aaacttctgt tcattttgtt catcggtctt tttctgcaag
     7141 ccatcgtcat ttctttgttt atttatcatc gatcaaggga tgcctatatc cagctgttca
     7201 atcaatcgaa tgatgtggta ctaaaaaaga ttcaatctga atttgaaagt ttgaatgata
     7261 caatcgagaa tacattagcc gcttttgatt ctaatccagc tgtcaaaagc tatttttcca
     7321 atgatccagc ccaacatatg gaacagcttc agcaactaag aacaatccaa aaaatgaatg
     7381 actctctttc aaaaatccat ccaatgatcg attacgatgt attgatcttt ggagaaaatg
     7441 gaagaacttt tgtcggaaac gacatgctca ctgccgtatc tgccgattct ttttttcaat
     7501 ctgcgattgc tcagagagtg aatgaacgtg ctgctgatac acaaatgctt tttgcacacc
     7561 acggattgac tcttcgcgat aagaaagcac ccagcgtctt ctttgtcaaa aaactgaaaa
     7621 atacattgaa tcacgtttac ggatatgctg ttttatcgat ctcatccaag caactagcga
     7681 atctttttca atctgtagta aatcctgaga tatcgaagat ctccttcatg aacgaagaac
     7741 agacgatcat cgcatcaaat gaagaaaata caattgggag ccgatcaatg attttcaaaa
     7801 aactaacagt aggtgaaaca acgatttcaa atggacagca gttgacccgt cttcctctgt
     7861 accgccagga ttgtacatta ctcagtcaag tcgatcttca ttctttatct aaccaaatgg
     7921 gcgtgatttt tccgattatt ttgtataata tcgtgacact tgcttttgct ggctcattgg
     7981 tatttatgta tttggatcga cacacaaaat cgatctaccg cttgattgat tcgctaaaac
     8041 ggctagagga agatggctta aaagctactg ttcctgtaca aggaagccat gaagtccaat
     8101 tgctagggaa tacgatcaat caattgctaa gtaatgctca gcatcattat gaacagacga
     8161 tccaagacga aaagaagaaa cgttcgcttg aaatccaaac tatgcaagca cagatacagc
     8221 cccattttat ttataatacg ctgactagct tgaaattttt gatttggcag aaaaaaacag
     8281 atgaagcagt tcaaggaatc gacagtttca ttactttact aagaagtacg attgggaaaa
     8341 aagaagaagt gattccagtc aaggaagaaa tcaaaagcgt acaaagttac atcgatcttt
     8401 tgagtatgcg atatggcgaa agaatctcgg ctaaaataat gattcctgat gaactgcagt
     8461 cattatctat gccgaatatg atccttcaac caattatcga aaacgcctat cttcatgctt
     8521 ttcacaataa aacgcaagga ttcatcacgg tgtacggcaa aacaaaaaat cagcttcttt
     8581 tattcgaagt gattgataac ggcgatggct tcgatactga aaataccaaa caaaaaaatg
     8641 atcatttttc aggaatcggc atcagccatg tagatgagcg gatacgccta atgtatggcg
     8701 atgcatatgg cttaacgatc caatcagtga ttgggacagg aacgactgta acgatttgtc
     8761 taccgcttct tcctaaaaat caatcagaat atcaaaaata aagaatagcc aagcgctgat
     8821 ttttttattt taagttaaat aaatcaatca gtattcttga ctttcatcta cgcgtctctt
     8881 ctactgtcag cactggataa cagcccaata tttgactttc tcctccgagc atcttgattt
     8941 cttggagcgc attctctact aatgtcattg gacggtccaa caacaaatcg atcacgaaaa
     9001 aatattctcc caagctagtt ttcaatggtc gggattcaat cttacttaag ttgatttccc
     9061 gccagccaaa agctgccagc attttatgaa gcatccctgg ccgattagca ggtagtgtca
     9121 agatgacact catttttact ggagctgact gctggctggt catctttcgg tcaccgacga
     9181 tccaaaaacg tgtctggttc aactcattat cttggatatt ctcagctaag atctccaatc
     9241 caacatgctg tgctgtttct aatgaagcaa tcgctgctgc gtcttctttc ggatgttctg
     9301 ctacatacat tgctgcagca gttgttgatt ctgttgctac taaaggaaca ttaggataat
     9361 gcgtttctaa aaactgttgt gattgtgcta gtgcttgtgg atgtgaaagg atttttgtaa
     9421 tctttgttgc tggattgcct aaaagctgct gcttgatagg aagaacgatt tcacttttaa
     9481 cttcgacttc tggatgtttg gaaagaagat cgattgtgtg atgaacagct ccttccaatg
     9541 aattctctac aggaacgaca gccagatcga cttgtttgcg aaacaaagcg cgcaaacaaa
     9601 cagggatgga agcatacgca gtcaattgtt ctgtaggaaa tagctgacaa gccgcctgat
     9661 aagtgaatga actttctggc cctagatagc tgacgttcat atgcttttga tcctttctat
     9721 aatggtatcg atgatctttt ctggtgtttg attggtcgtg ttgattcgat gtgtcgcact
     9781 ttcttcatac caagaaagtc gttgtgccaa taatgctttt atttcttctc ttgtttttcc
     9841 aagagctaaa ggtcgaatag acgtttcatc ttgttggata cgatcaacta gacagtccgc
     9901 ttctgcctct aagtaaagaa caatcggctg tgtttttaaa aaacgccgat tttctgcact
     9961 ttggacaatc cctccaccag tcgcgataat tttgtcttca tttgacagct ttcttaaaat
    10021 atctgactcc acttttcgaa agcttttttc accgtaatag tgaaaatatt cggcaatcga
    10081 caattggata tgttcctcaa tctttgtatc taaatctaca tactcttttt gtaaacattg
    10141 agctaaccct ttaccgattg ttgtttttcc agcgcccatg aaaccaatca gcaaaatgct
    10201 agccatgagc ttctcctcct gacacaagtt ctgctaaatc gtcaaaaaat gccggataag
    10261 agattgaaac agcttcagct ccttctagtt cgcaaggtcc atcagctatc aaagcagcta
    10321 cttgcagcat cataccgatt cgatgatcgc ctctgctgga cacttttgcg ccatgcagag
    10381 gtgtcggccc atggatgatc agaccgtctg ctgtcgcttt tatctttgct cccattttct
    10441 gtaattcttc tgccaccgca tcaattctgt tggtttcttt tacttttaat tcttctgcgt
    10501 ctttgatgat cgtgattcct tctgcttgag tagccactaa agctagaatg ggcaattcat
    10561 caatcagacg cggaatgatt tctccaccaa tcgttgcttt cttcaaatga ctcgtcttga
    10621 cacttaaatc agccgattgg ttctgttcat tgtgattcgt ctgggtaatc gctgcgccca
    10681 tctcctccaa cacatctaag ataccagttc ttgttggatt gaccccgaca ttttttaaca
    10741 acagttggct ttccggaaca agaagtcctg ctgctagaaa gaaagcagct gatgaaatat
    10801 cacctggaac agttatctgt tgaccagtta atttttgcgg tccggtcact atgatcgttt
    10861 tatcttccac tgtgatcctt ccgccaaatt gacgaatcat ttcttccgta tggttacggg
    10921 aacgttcttt ttcaacgatt acggatgtgc cttctgcttg taatgccgca aacagcaaag
    10981 cagatttgac ttgcgcactg gctacaggca tattgtatcg gataggtgat aaagagcggg
    11041 ttcctgtcac agtgattggt agatactggt cattttcttc tccttgcaag tcagcgctca
    11101 tctcgcgtaa aggttccatc actcgcccca ttggacgttt agacaacgaa gcatccccga
    11161 acagagtagt cgtaaaaggc tgtccagcca aaatacccat caataaacgt atggtcgtac
    11221 cggagtttcc catgtcaatt gattcggttg gttggacaaa tgagtggcta ccgcgaccat
    11281 gaatcttgat cacctcttcc tcttcttcga tctcgacacc taattgttgg aaggcatgta
    11341 aagtactcag acaatcttct gcgcgcaaaa aattttgtat agtcgtcgtt ccttcagcaa
    11401 ttgctccaaa catgatgctg cgatgggaaa ttgatttatc tgctggtatc ctaacagtcc
    11461 ctcttaatcc atgtatttgt tgtaataatt gcatttatcc agcctcctat ttgatccagc
    11521 aatgataact ggtattagac ataatgattc tttttgcttg taacaaatcc tgttggtttt
    11581 taaaactgat ttgcagtatt cctaaaatat cttccctggt ctcttggatc ttcaaattaa
    11641 tcaaagaaac tcccgctttt ccgatcagtc cagttacttc tgcaataacg ccaggttcat
    11701 ctggcacatc gataaataaa tcaaaaaagg caggaatcgc gccttgctta tggacaggca
    11761 actggtctcg actttctttt gcccgattga aaaaagaaaa gatttccgat tggttctcag
    11821 tcatgatcca ttctgctatt tgtgtcattt cttgttgcca atctgccaat cgttccaata
    11881 atatctgctt attgctcaac aaaatatctg tccacatgac aggatctgag gaagcgatcc
    11941 tagtgatatc tcgaaagcct cctgctgcca gttgtttggc tcgcggatac tcttgattga
    12001 attgttctgc ctgattgact aagccagctg ccagaatatg tggaaaatga cttagcattc
    12061 ctgttatacg gtcatgttct cttgctgtaa gagttacata ttttgctttg gttccgcaaa
    12121 atagttcttg cagtttttca acatcttttt tcgtcttttc caaaggtgta aagatgtaat
    12181 aggcattttc aaatagttta gcatctgctg cctgtatacc tgatttatga gaaccagcca
    12241 ttggatgtcc accgacaaac cgaagatttt tctgctcagc ggatgtcacg acttgctgct
    12301 tcgtactgct gacatccgta atgatgacgt gatcagacaa gttgattctt tcaaggacat
    12361 caagataagc taaggttgta cggacgggaa cggctaataa gataacgtct gcttgttctg
    12421 cacctgtttc aaaatcactt gcaacgtaat caacgatctg ctgttctact gcttgctcac
    12481 gggtttttga ctgactatcc caaccaacta ctatcagttc tggattggtc tgacgaatac
    12541 aaagtgccat agaagcacca atcagtccaa gccccacgat aaaaacatgt tgtgccatct
    12601 tatttgcccc ctttttagta atgtttcaag tagtcacggt actccttcac tgcagctttc
    12661 aactcaacaa gcgaatcact tgggaatttt gacaatagct ctgtcgctac cacagtggct
    12721 acaacatttt ccgcaacgac gctagctgca ggaactgctg tactatccga acgttctaca
    12781 cttgctttgt aaggttcttt tgtatcgata tcaacacttt gaagtggctt gtacaatgta
    12841 ggaatgggtt tcatgacccc gcgaatgaca agtggttcgc cattggtcat tccgccttcg
    12901 aagccgccta gattattggt acgtcggaaa tagcctcttt cttcttccca taggatctca
    12961 tccatcactt ctgagcctgg taatgaacca gaagtgaatc cgaccccaaa ttctacgcct
    13021 ttaaaggcat tgatactaac cacagcttgt gcgattttgg catctagctt cgtgtcccat
    13081 tgtacgtagc ttcctaatcc agctggaacg cctccaacga gcacttctac aattcctccg
    13141 atcgtatctc catttttctt tgtctgatcg atcaaatctt ttattctttg ctcaactgct
    13201 ggatcaacaa ctcgtacttc tgacgcttca gaaatttctt tgatttcttg aatcgagata
    13261 tttccaggta tttctgcttg gattccacca agacaaatca cgtgtcctgc tacgatgatg
    13321 tcaagttctt ccagaatttt cttggcaatt gcgccgattg ctacacgcat ggtcgtctct
    13381 cttgcagagg accgttcaag cacattccga agatctcgat gatggtattt cataccgcct
    13441 accaaatccg cgtgtcctgg acgcggacgt gcaactcgcc gcagtgattg gtctttttca
    13501 ggcacttctg ctacagacat gacagttgtc caatttttcc aatctttatt ttctaccact
    13561 aaagtgacag gtgaaccaat tgttttgccg tgacggatgc cagacgtgat ccgaacacga
    13621 tctttttcga tcttcatcct tcctcctctg ccgtaaccag cttgtctgcg agcgagttcg
    13681 tgattgattt cttcttctga cagaggtaat cctgctggta gtccttcaat aatggctgtt
    13741 aattctggtc catgggattc tcccgcagtc atataacgca tcttacagtc ctcctaagta
    13801 atctttgaat gtctctgtgg gaacaggaac gatttttgct tgcccaatct tttctagcaa
    13861 gatgatattc aattggcttc ctcttgcttt cttatcatgg gttatcgcgt ggaataattc
    13921 atctggatga cttagatcgt acgtaatcgg cagatggaat ttttgaagta tcctttctaa
    13981 agcagctgtc gatccagttg gagacaagcc gattttttct gcatgagaag tgatggctac
    14041 cattccaata gctactcctt ctccatgact gactacccca tatcctgctg tattttcaat
    14101 tgcatggcca attgtatgac caaaatttaa aataagtcga tttccttggt caaagacatc
    14161 ttcttccact acttttcgtt tgatttccaa cgtagcagta atgattggaa cagcatgttc
    14221 cagaagatct ttttcgtctt tcagctcgtt cagctgtttc caaagcgttg catctgctat
    14281 agctgctgat ttgatgattt cagcgattcc ttctcgtaaa cgacgtaccg gcaaagtttt
    14341 tagaacctca ggatcaatca atacaccagc tggctggcta aatgttccga caaggttttt
    14401 cgctttgttt gtattgactg ctgtcttccc gccaatacta gaatctactt gtgcaagtag
    14461 cgtagtcgga atttgaacga aatggatccc acgcatgaac gtcgaggcca cgaatgctgc
    14521 tagatcaccc accattcctc caccaagtgc aagtatccca tcgctgcggg taaaatcatt
    14581 atctgctaaa aaatcgtata actcagctgc ttgttctaaa gatttactgg tctcacccgc
    14641 cggtacgatt gaaaggactg gttcaaaatt agcttgtctc aattggaaca tgattttttc
    14701 tgcatatagt ggagcaacgt ttgtatcagt gatcacagct actttttgag gtctccaaat
    14761 cgtttgaagc catgatccag cctgttctag tgcgcctttt tcaatatgaa tatcgtattt
    14821 ttggttatca acatgtacag taagcattcg tttcacttct ttcttttaaa aagctggaat
    14881 taatcgagtg tctgaatctt ttccattgct tctttcatga tcgcaacttc tttcatcatg
    14941 cgtgagtaag tcttttcatt caacgattgg ggaccgtctg accaagcatt tgcaggatca
    15001 ggatggatct ctacgatcat gccatctgct ccgcttgcca cacccgcacg agccattggt
    15061 ggtacaagtt cccaaattcc tgtaccatga cttggatcaa caatcacagg gaaatgagtt
    15121 aattttttca tcaatgggac agcacttaaa tcaaacgtat tacgtgtcgc tgtttcgtaa
    15181 gtccgaatcc cacgttcgat aaaaatgaca ggagatttgt cttcgactgc aatgtattct
    15241 gcagcattaa gccattcatt gatcgtaccg gaaattccac gtttcaatcc aacaggttta
    15301 cctgttttac cgacagcaga tagcagttta aaattttgca tattacgtgc gccaatctgt
    15361 aggatatcgc tgtactcagc caccatatcg atatgccctt catccatcac ttcagtaatc
    15421 actttcattc caaattcatc agccgcttga cggatgtatt tcaatccttc ttcttctaaa
    15481 ccttggaaag catatggaga agtacgtggt ttgaaggcac cacctcgcag aatttttgcg
    15541 cccccggctt tggccattcg cgcacattct ctgatttggt ctaatccttc gatagaacaa
    15601 ggaccagcca tcgtcacgaa actgccgtca ccaattttta ctccgtctac atccacgact
    15661 gtattgccag gatggaattc tcgagaagtc aatttgtatg tgtttaaaat acgcacagca
    15721 ttttctactc cttcatagcg tgtaaatgct ccttcttgta aacttcgcgt atcacctttt
    15781 agtccaatca ctactcgttc tgttccttga tcgattgaga ctttgaatcc ttctttttcg
    15841 atatgatcga cgactttttt tatttgttct tccgttgctt cttttttcat aattactatc
    15901 attgtttttc ccgctttctt ttgtttactt atctatcttg gctgattttc tctacttttt
    15961 caaggtggtt ttacattaat tcctactaaa tgaccgtgtc ctgttgttct cattttcgct
    16021 ccttctttcc tacttatgac ccagataatc aacaaaaaaa gatgtccctg taggaaattg
    16081 cctctacagt gaacatcttc aacgagcctt aaaaagcgct gcaaaaatat aaaatagcca
    16141 ctatagatga atttctctac gtggctgtaa gtcgtgttta aaaatcttta gccatcatag
    16201 atacaaacct ggtttgtttg tacccacgaa gtcatgaata caaacctatc taaaatagct
    16261 aaagtaatac gtattcagtt ttgtaagttt tctagatttc ataaaaaatc gctccaaact
    16321 gatgtaagaa ttgtacctag tatagacata aaaaaatcga ctgtcaatca tttttatgaa
    16381 acttttttta attttctgat agttaacttt gtttttcaga aaaataacaa ttggttttat
    16441 tgaagaaaat agtcatgaac aaaagtatct gagaactttt ttactctctt gttcatgaca
    16501 tatagaataa agcagctttg tcttgtaaac tccgtttttc aaataataat ctaaagattg
    16561 ctactgaata ataaagccga tttcatggtc ctcttcgctg ctgattcggt aaggtgactc
    16621 aacatccgct ccccagctat tgataccccc gactcctctt acttttgcaa aaatcgtaag
    16681 aaccgttcgt ctttctggcg gtagttcttc tatatgtgtt gcctgttcca gttcttctgc
    16741 tttgaatggt aaagcggaaa aagcaaatgg cgtatcctgc tgttcaaatc gcaacgaaaa
    16801 atcttcttga tctttatccg tattatccaa cgtttgattt cttgttagtt ctacccattt
    16861 cgtttccata tgcatgccgc attcttgggg aacaaggtat ggggaaacgg ggagtcctgc
    16921 cactttataa ataccttcga caccaccagc catccgatcc ggatatgttt cccctgatag
    16981 gccttcatac tcaaaaaatt ttgctttcgt tggcataatg aaccttaaac ccaatgcggg
    17041 taattccggt aatccttttt gtccataata atgcatattg acaagcattt taccctctcc
    17101 agcgatttgg taggaaattg tcacttgggt ttttggtact gtgttgattt ccagtgtaaa
    17161 aatgatttca accgtttcag cgtattcatt acttgaatat tgattattta ctggagcaat
    17221 cggcaaatcg atctttttat tgtccaccag tacttctatt tctttgactt ttggatacaa
    17281 tccggctcct aaccacatac cggatttaaa tccaaatccg ctacctctgt cattatcggt
    17341 cgttgctcgc caaaaaactg gaagtgtttc ccggtatagc cattcttttc cgtttttatt
    17401 gagcgattcc agtccgcctc ttgtataaga aaaaatataa tgcgtattcc ccgatttgac
    17461 acctaatgta cagtcgccga agacgattgc aactttttcc gtatccatat gacttttatt
    17521 tactgtattt ttcataataa tatcttacct cctgcatggc gggtttttct tgtccatctg
    17581 caaaaacgat cccattccct gagaattcat agtcacttgg tttatcgtca aagtcaccgc
    17641 catagcgcat cacttctctg cctgtgattg gatcagtcac ttggatcgcc tgatctttaa
    17701 aatcccatat atagcctcct tggtacattt cataacgatc aagcaacgtc atgtaagaat
    17761 ccattccacc taacgaattc cccatgtcat gcatatattc acataagatg aacggttttt
    17821 ccgctttatt ttcaaggtag tcaatgatct cttctggtgg cgcatacatg cagcttttta
    17881 tatctgaaat cgaatcttcg aatgctggat ttggaaaatt cccttcataa tggacaagtc
    17941 gatcggggtc tacttttttg aagtaatcgt tcatttgtcg aatgttttct tccgcataag
    18001 attcatttcc aagagaccaa aaaagaatac ttggatggtt ttttaatgtt tcgaaattcg
    18061 tttttgctcg atcaagaaca gccgcctgcc actgaggtac tgcacctggt acgttgtaag
    18121 aaggctcaaa tgcgcccatt ttttgccatg agccatggga ttcgaggttc gtttccgcca
    18181 tcacatggat tcctgcttgg tcgcataaat aataccaagt gatctggttg gggtaatgag
    18241 atgtccgtac gccgttgatc tttgcatttt ttatgatagc caaatctttt tccatatctt
    18301 ctatagtgat ggcgcgtcct tttttcggat gccactcatg tcgatttacc ccgttgataa
    18361 tcatccgttt tccattgaag tagatgattt tatctttgat ttcgatttta cgaaaaccga
    18421 aggaatagtc aatatactcc gttactcttt tttcttcgtc tatcgtctcc aattcaagct
    18481 tatataaatt gggctgaaaa tgcccccaca gtgccacatt ttcaaatcca tcgatgcgaa
    18541 tcgtcatttg ctcttttaac ggatattgct gttgatagat ttccttccca tcaggatcta
    18601 gtatcgctac attcagctga ctgtttttgg ctacttgctc aaaagacaaa tctacttcaa
    18661 gtttgccatt ttgacatttg tcatctaagc gaggtttgat ccaaagatct tctaaatggt
    18721 tttgtcctcg agcaactaag aagacatctc tgaagatccc aaaaaatcgg aaaaagtcct
    18781 gatcttccaa gaatgcagct gtacttcttt tgtaaacctc cacagctaga atattttctt
    18841 cttccaaata aggtgttaaa tcaaattcag acggagtgaa cgaatcttcg gcatacccga
    18901 taaaatgacc attcaaccaa ataaacattg cttgttctac accagcaaaa tgaatcgaga
    18961 tttgttttcc ctttagacct tcttctaatc gaaaagtcgt caaatacgaa ccaaccggat
    19021 tgtctttagc agctgaaaag atcccttccg cctctttctc agggcttgtc gtgaaagggg
    19081 gacggcgaaa tacatgtccc tcccacggat acatcgtatt gatgtaatgg atcttgtcat
    19141 aatttgctaa ttcaatatga ctaggaacag tgatttcatc aaaagaacag gcatcaaaat
    19201 cttttttata aaatgttttt ggtcgtaccg aaggatttgg cgaataacaa aaacgccatg
    19261 atccatttaa agactgttta agggaggatg tggtttctac ttcttccctt gttgcataac
    19321 aaacatgatc gctatgtgca tttgattggt tcactctaaa aacttgtgga tcatctaacc
    19381 attttatatt tgctttcata cttttttcct tctctctcat aactttttga aatgaagcag
    19441 ttctgtttgg aaatcacctt gaaaatcggg gaagtaaagt ccaactgtca tcaattcggc
    19501 ccccgaaaat atccgcttcg aggtctgttc ctgataaagt gcctcatcat ctaaccccat
    19561 cagatatact tcatggaacg ctggttgagc tgaagctagt atcctcccta aaaacacaat
    19621 agcttcatct tgtttaggag acacaaacat ccatgcggca atattttctt caaaaggact
    19681 cgccaaacga taataatggc catactggat caccggacga atcgtttgat aggatataat
    19741 ttggtctttt acttgctttt tttcttcagg agacagcttt gtcaaatcca gttcataacc
    19801 aaaaacagcg ctcatagctg tttctcctct tgttttcaac gaagtagatc tgtcggtctg
    19861 atgatttggt acagctgata catgagctgt gatggacgaa atcggataag caaggcttgt
    19921 tccatattgg attttcatac gctccactgc atccgtgtta tcacttgtcc atgactgcgg
    19981 catataataa ataaacccgg catcaaaccg tccacctcca ccagaacagc cttcccataa
    20041 gatttccgga taatcagttg tcaaatgttc aagtagttca taaagcccga gcatataacg
    20101 atgcgctact tctccttgtc tctgtgggtc aagggtaatc gaatagacat ctgaaagcga
    20161 acggttcata tcccatttga tgtagtcaag aggaatcgta tctaaaatct ttctcatttg
    20221 tttctcaatc gtctgccgaa catctaacct tgtaaaatcc agtacatgtt gtgatctgga
    20281 agcagaaggc attctacctg gctcctgcat aagaaaatca ggatgtgttc gatacagttc
    20341 agaatcaacc gagatcattt caggttcaaa ccataaacca aatttcaatc ctttgctatg
    20401 agcataatct gctatttcac gtaatccatt tgtcaacttg ccttcatact cgaaccagtc
    20461 tcctaatgaa gaattgtccg catctctttt tccaaaccag ccatcatcta atacgaacat
    20521 ttcgatgcct agctcctttg cttcatcaat gatcgccttg attttttcac tattgaagtc
    20581 aaagtaagtg gcttcccagt tattgaccag aatcggccgt tcagcaaatt gatgacgacc
    20641 tcttgcgatt ctttcacgta gcaactcatg gtggatctga ctcattttgt tcaatccctg
    20701 acttgagtaa gaaaggatca tttcaggtgt ttgaaaagac tggcctgcat tcaattccca
    20761 cgaaaaacga tggctgttga tcccaccgac gactctcaat tgattgatct gatctttttc
    20821 aagttcgaaa gaatggttgc cggaataaac aaattgaagt ccgatcgctt ctccagtatt
    20881 ttctgtcgta tgatgatgaa ctaaagcaat aaagttattc atatggtgac tgctcgttcc
    20941 tcgacggctt tcaaatactt ttgttccaga taaaacagat tgccgtgaaa tctgtctttc
    21001 attgacatgt gctcctggca aagcaatgac ttcatcaaat cgcctagtat gggcaaagtc
    21061 tagctggaaa ctagccgctt tttctagttt gattgtttct ccggtttcat ttcttatctt
    21121 tacagatcgt gtgatcactg ctctatcagt aaatatggta tagaacaaat cgaaataaat
    21181 cgctgtttct ctatccttaa gtgaaatcac caacgtttgt gcctctgact tgtccatcac
    21241 ataagtttga ggcaagcctt cgatctctgg cttcccatct gtaatataat ggctgtcata
    21301 tctcagatcc agtaagtctg atccattctt gcgctgaatg atcgatgcgg gcgttcgaaa
    21361 gtccatgctg ccatgagaag agtattcttg cggtagtgta tctttcgaca gagaccggtc
    21421 aggatttaag ggaacatttc ctgaaaatcc acgatctctt cggggataat ttttattgtc
    21481 tttgtactgc ttgactggtt tgccaaaata aatatgcgcc aatacattgc tttcttctat
    21541 ctttatcaga taagaaagaa atgtatttcg taaatggaag actcggctct tttcatcaaa
    21601 taagatgaaa tttttatctg tttttctatg cgtaatatcc atcatttcac tcccaacaaa
    21661 acggaaaccg tttacttttt tttataattc ctgtatactg tttactaaac aattttgtca
    21721 ataaaaattt tttatttttt tgaggagtgt gtaaaaatgg ttggaattag agatgtcgca
    21781 cgaaaggcaa atgtctctcc tgctacagtt tcccgtgtgt tgaataacga tcagacgatc
    21841 catgtggctc agactactcg gcaacggata gtagaggcag ctgctgcgtt ggattatgac
    21901 atcagtactc gtaaatatat caaaaagaga atgccttcga ttggtgtgat ctctacgatc
    21961 ggcaaagcga gcgaagaaca ggatatctat tacaaacatt tgcgagtagg gatggaagaa
    22021 gaagctcgac gtctgcattt agggatgaat cgaatctata atctatctga taatcctaaa
    22081 caatgggaag atctagatca gttaggtgcg gtgatcgttg ttgggactgt tacgaaagaa
    22141 tcgattaccg ccttgttaaa acaaaacatg caaatagtcg tcgtggataa ccctgacatc
    22201 cagcaagagg tcgatatggt gtatgcagat cttgaacgga tgactaaaac cgtactggaa
    22261 ttgttcttag aaaatggtca taaaagaata gcctatatcg ggggatataa agtcgatatg
    22321 gatgaattcg gtgggaagca gatttctata aatgaaaaga ggatgcgtgc ctatcagcat
    22381 tttatgatcg atcatcactt agaaaaatat atgtcttatc atctaggtgg ctggacagaa
    22441 aaacaaggtg aggtacttgc gactaatcta ttagaaaatg aatcccctct tcctactgcc
    22501 attcttgtcg ggagcgatcc aatggcaatt ggtgtctatc gggcattgaa acaaaaaaat
    22561 atccaaattg gagaagagat tgttgtagca agctttgata atatcgagac aatcgcaaac
    22621 ctctcccctg gcttgaccag tgttcaaatc aatgcgaaat caattgggca agcagctgtt
    22681 cgaatggcat tggagcgaat tgacggtata cgggacgaac caatcatttt gacttatcct
    22741 gcacgattga ttgttaggga aagttttatc ccaagaacaa aaaggtattg aaagagaggt
    22801 tgtgaaaagc cgcttcgttt cttcatactc aaaacgaaac ggctttttca caacctctta
    22861 cttatactta ttcacaaaat ccaatgtctg ctggtaacaa ttttgcgatt cttttagcgt
    22921 gtaatcaaat tggtattcat gggtaatctc tttttttgta tctgtataaa acaaggattg
    22981 cacgggtacc tttaattctg tcaaccgatt ttctaatgct tgaccttgct cctggaatga
    23041 gtatgcattt ccgtcagtta tatacgttgg cggaaattcc ttagaaacat ggtcagccac
    23101 actagcttct ttcaattgct cactcgtttt ccaatctttt gttccaatca acgaccacgc
    23161 aacggttttg acgaaaaact tcataaatcg atcatccgaa tgctgctgtg ctgttagctt
    23221 caaatcaaca ggtccgcaat aagaaatggt tcctttcaga ctatcttttg gaagtagctg
    23281 tttgattttc atttgtttag catacgcatc attcgtttgt atagctgcat attgcaatgc
    23341 gatttgtgaa cctgcactat ctccaccaaa aaataattgt tccatgtcta gcaatttgtc
    23401 gtctttttct tcagctaata aatctttgat cagttcattc acttgaagaa cttgattagg
    23461 gtattctgaa tcaggcgcta attcataatt catcgcaacc actgcgatgt tggcgtctga
    23521 aactaatttt gtcgcaaatt ctttgacacc cgatttatct ccgccgacga atcctccacc
    23581 atgtacccaa aatagaacag gaacaggccc cttgctgttt tttggatagt agatgtcata
    23641 agtattctct ttatacttgg actcatacgt caaatctgtt tttttcgcca cgtttttttc
    23701 tgcttgatcg aacgtttttt tatcagtgat ttgaacagta tttgaaaaca tatggttgat
    23761 caagaatgct ccaggtctgg gggaaagctg gaaggcaaga aagattgcaa acgcgatccc
    23821 aagaatgatt ccgaagaacc atccgataaa tttaagaatt ttctttttca taaggctccc
    23881 tcagtagtta gtattaagca ttctgattat accatagtgt tatttcctat caagctctcc
    23941 tagatgattt aatgattttc cccaaacttc ttctgcaatg cttttgacaa aagaaatttt
    24001 ttccattgtt cttcaaacaa aacattttct tcttctgttg aagcaaatcc acattgagga
    24061 cttaagcaca aacgatccaa aggcagatat cgtgtcgctt cgtagatccg gtcaatcact
    24121 tgctggtgct cttccatttc gccatttttc gttacagtca aaatctaaga caactaattt
    24181 atcttcagag actttctcta aaagaacaaa actcccagca cgttcactat cgtattctaa
    24241 ataataggca cggacatttt cttgagtaaa ataatgggtc tgctacgtcg tcatcccccc
    24301 tgatgccgcc aaaatcgaat aataatttcc gcgtcaaaca tgtatattta gtgttgaatc
    24361 atttgagaat atgaaaataa aaatcttttt gatcaatcag ttaatcaaat tgaaataacg
    24421 aaaaatctga gacagaatga cgttcattct gacccagatt tgattttgca caactcaaca
    24481 gtccttattg gcaaatcgcc aaaataatgt agactaacta aaaagactaa gaaacaataa
    24541 caaaagttaa cacttcttag tctttcttta gcacgtctat ttattaaaga ttttagggcc
    24601 ttttctcatg tttttacgat cattcttagg tgtttccatt tgtttggatc ggcctccacc
    24661 ttgtttcttt ttgatcaatt ctttcatttt ttctttttgg tccattttta ttctccttta
    24721 acgaatgaag cgactaattc gatatgatgg gtttgcggga aaagatctac aggctgaact
    24781 ttcttcacgg cataacctgc ttctgcaaat aacttagcat ctcttgcaaa agttgccgga
    24841 ttacatgaga tatagacgat tcgttctggt gacatttcga ttgctgattc aataaaatga
    24901 tgatctaatc cttttcgtgg agggtcaact acgatcacag atggtttgat ccctttttct
    24961 tgccattttt tcataacttt ttctgctttt ccgacttcat aatgaacatt ttcgatttta
    25021 ttatttaaag cattgaactg tgcatcttca attgcttctg gaataacttc catcccatac
    25081 accttatcca ctctgtctgc taaagacaag ccaatcgtac ctattcctga ataagcgtcg
    25141 atcacaatgt cttttttatc taactcggca aactcgatgg ctgtttggta cagatactct
    25201 gcttgttgag tatttacttg ataaaatgac tttgctgaga ttcgataggt ttttcctaat
    25261 agctgatctt cgatatagtc tctgccatat aaaacttttt ctttgtctcc taaaatcaca
    25321 tttgtttttt cttcattgat attttggatg accgaaacca cttctggcaa ttcttcatga
    25381 atagcttcag caatttcctt ggatttaaag aatttttctt tacgagtaac taaaacgacc
    25441 atcatttcat gggaataatg accacgacgg ataatgatgt gacgaagctg tccttcattt
    25501 ttttcttcat tatatcctcg tacttggaac cgttgcaaga tatctctgac tactttgatt
    25561 gcttgatcga tgacaggatc ttggatataa aaatcttcga tagggactag atcatggcta
    25621 ttctttttgt aaaaaccggt ggctaactgt ccatcgatac ggcgaacagg gatctgtgct
    25681 ttattacggt agccgaatgg ttctgccatt ccgaccgttg gaagtacagg aacttctggc
    25741 atcttagcaa tcttcgccat tacatgctct acttgttttt gtttgaagac aagctgttgt
    25801 tcatacttca aatgggctaa aggtgcgata cctatgcgaa gtaatgcatt gttttctaca
    25861 ggctgacgat caggtgattg tgtaataaat gtttcgactt ttccatatcc aaacttattg
    25921 cctgctttta ctactagtat ttctacttct tctccaggga gtgcattttc gataaataat
    25981 ggataaccat cgatttttgc tacccccatt ccttcataac tcaagtcgac gatttcgact
    26041 gtatatcgtt cgtttttctt tactatttgc tgttctgtca tattttgctc ctttaagcct
    26101 atttttcaat cctactgaac tctactataa atcagctaat tacacaacta aaaccggcac
    26161 tgaccttact acgttcagca ccggcttttg attaattctt ttgattaggt gtttcgtctg
    26221 ctatttttcc gtcaccgtcg atatcttcat ccgttaaacg ttcgacttct tttacgaatt
    26281 ctttgctgac aacctctaat tcttcttcat ctgtacctgt gattgcttca tcaggaatct
    26341 gatcggtatt ggcaaagaac tcaatgtgtt gatgaagatt ttggaaatgc atcggtgctt
    26401 ccccgccgta ttcaccatct aagttgatca ttactggtcg attgatatca tcttctgttt
    26461 caacgaacaa actgcttgtt ttggtataga ttattcttgg atcatcgaca tgtcgaccgc
    26521 cattcagcat gagtgctgca atatgcaaga tctcaaagat atttgctgtc ttcacaatga
    26581 ttaaagaaaa cttcccatca tccaatttgg catctggcgc gatctgttcg aatcctccaa
    26641 cagagttcgt cagccctagg aaaaacatcg aagcatttcc gatatattcc ccttcatcat
    26701 acttcaagcg cattttgatt ggctttactc gagggagcat ctctgctcct ttcgcaagat
    26761 aagcaagata accaaaaata ctctttagtt ctgatggaac ttcataagtc aattctgtca
    26821 aatagccacc tgcagcgata ttgatgaagt aactgttatc agaaaggccg atgtccattt
    26881 ttacggtttg gtgttttttt atcacttctg ctgctgcttt aatattatct ctaggaattt
    26941 ttaatgctcg agcatagtcg ttcgttgtac cagctgggat gatggccatt ttaggacggt
    27001 gttcaagcgg agcgattcca ttaaccactt cgttgattgt gccatcgcca ccagctgcca
    27061 ctactagatc aaaacctagt tctgcgactc tacatgcttc attcttggct gaatctggtt
    27121 ctggtgtagt tgcataggcg ctggcttcaa atcctgcttc ttctaatgcc tgtaggatat
    27181 ccgccaaatt ttttttcaat aattcttttc ccgaggtggg gttataaatt acgcgtgctt
    27241 ttttcatttt cagcctctat cgtttcgcta attcatcttt aagtagctta ttcacgacac
    27301 caggatttgc ttgtccttta gttgctttca tgatttgacc gactaggaag ccaaccgcac
    27361 gatcttttcc atttttgaaa tcatcgacag attgttggtt gttatccaaa atttcattaa
    27421 tgattggcag tagtttgttc ggatcagata attgcaccca accatttttt tcaactactt
    27481 cttgggcttc tccgccattt gtgatcaatt cacggaagac ttttttggcg atttttgagc
    27541 tgatcgttcc atcggcgatc aaacgaatca ttcctgctaa gttctcaggt gtcaattttg
    27601 tctcagctaa ttccagtttc tcactgttca agtaagcaga aacttcaccc atcaaccagt
    27661 tagatgcttg cttggcatct gcaccatttt ccaaaacagc ttcaaaaaaa tcagacattt
    27721 cttttgtaag tgtcaatacc attgcgtcat actctggaag tccaaactca cggatatagc
    27781 gttcgcgacg agaagctggc atctcaggca aactagcttt tactttttga atccattcgt
    27841 catcgatcac gaatttcggg atatcaggct ctgggaagta acgataatca ctagaccctt
    27901 ctttgacacg catcaaaact gttttattcg ttgtttcgtc aaaacggcgt gtttcttgtt
    27961 ggatttctcc tcctgaaaga agaacttttg cttgacgttt ttcttcaaaa gctaaacctt
    28021 ttttcacgaa agataaagag ttcaagtttt tcaattctgc tttcgtaccg aactcttctt
    28081 gtccataagg gcgaagagaa atattggcat cacagcgcat agagccttct tccattttca
    28141 catcgctgac ttctgtaaat tggataattg atcgcaatgc ttctagataa gcgtatgctt
    28201 cttctggtga acgcatatca gcttcagaaa cgatttcgat caaaggcgtg ccttgtcggt
    28261 tcaaatcaac gtaagaataa ccgccaatac catgcatgtt ttttccagca tcttcttcta
    28321 aatggacacg ttcgatccgg atttttttct ttttaccttc tacttcgatt tcgatccatc
    28381 cgtcatgacc gattggttga tcaaattggg aaatttgata tgcttttgga ttatctgggt
    28441 aaaagtaatt tttccggtca aaatgagtat ctttagagat ttcacagttt aatgccaaag
    28501 ctgctttcat cccgaattcc agcgcagctt tattcattac tggcagcacg cctggataac
    28561 cccagtcgat cacgtttgta ttgctgtttg gttctgcacc aaaatgagct ggcgctggag
    28621 agaaaatttt tgagttggtt tttaactcga cgtggacttc tagtccaatg actgtttcaa
    28681 aattcattcg tctttccctc ctaaaatagc tggttgttgt ttatggaatt ctgttgcttg
    28741 ttcaaacgca tatgccgctt gatacatcgt tttttcatca aaataattac cgatgatctg
    28801 caatccaact ggtaatcctt ctgataaacc tgccggtaca gacatacctg gaagtccagc
    28861 caagttgaca gggatcgtta agatgtcact catgtacatt gtgattggat cgttaatgtt
    28921 ctcaccgatt ccgaatgcaa ctgttggtga agatggcccg atgatcaagt cataatctgc
    28981 aaagactttt tcaaaatctc gtttgatcaa tgtacggact tgtcctgctt ttttgaagta
    29041 agcgtcataa taacccgcgc ttaaggagaa tgtacctaac atgatacggc gttttacttc
    29101 ttcaccgaat ccttctgaac gtgagttgac atatacatca tctaatgatt tcacattttc
    29161 agagcgataa ccgtagcgga taccatcgaa tcgttgcaaa ttggaacttg cttctgatga
    29221 ggcaatgata taataaacag ccacaccgta ttttgaatga ggcaagctga cttcttccac
    29281 aacagcacct aatgaacgga atgtttctgc tgctttcaat acagcttctt ttacgccagg
    29341 ggccacacct tcgcctaagt attctttagg taaagcaatc ttcattcctt tgatgtcgcc
    29401 tgtcagtccc tctgcaaaat caggaactga aacgcctgaa gaagtaccgt ctttttcgtc
    29461 ataaccgctg atagctgtca atgctaatgc attatctttc accgtacggg tcattggtcc
    29521 gatttggtca agagatgaag caaaggcaat caaaccaaaa cgtgagacac ggccataagt
    29581 aggtttcatc cctacgatgc cgttaaaagc tgctggttgg cggatacttc ccccagtatc
    29641 actacctaaa gaaactggaa tttgtccagc tgcaactgct gctgcagaac caccagaaga
    29701 gccaccaggt acttttgttg ggtcccaagc attttttgtt ttcttgaaat aagaagtttc
    29761 tgtacttcca cccatagcaa attcatccat gtttagctta ccaacaggga tcatatctgc
    29821 ttgatagatt ttatccatga ccgttgcatc ataaattggt tcaaaattgt aaaggatctt
    29881 agatgcagcg gttgtcaaga tatcttttgt tacgatatta tccttgatac caattggaat
    29941 accagcaagg acgttgcttt catcaatccc tttttcatct aaagcttttg cctgttccaa
    30001 tgctttttct tcgttcaatg taatgaatgc ctcgacctca ggctctgttt ctttgattcg
    30061 ttcaaatgtt gtttttgtca aatcttgtac agtgatttct ttcgatacaa gcatatgatg
    30121 cagttcttct agagaacgat tgtgtaattc tgtcattatg cgccagcctc cccattatcg
    30181 atgattgcgg gtactttgat aaagccgtct tctgtttccg gcacattttt caataattca
    30241 tcgcggttcc atccagcttg cgcccgatct tgtcgcatca cgtttactgt ttcaaccaca
    30301 ttcgacgtaa atggaacacc ttccgtatct acttcttcca gcatttcaac catatcgatg
    30361 attttaccta attggtcggt aaaacttgca agttcttcat ctgcgaacgc aagttttgct
    30421 aatttggcaa cgtgtttcac ttgttcttca ctaatcgcca tactacccct tctttctaaa
    30481 cgagttaaaa actttgctct ctattttaac acaagagtgc cgtgtctgaa attgtttatt
    30541 gaaatttgtc tgaaaaaact catttttcat tcttctactg agcgactttt agccaatgca
    30601 tctgccattt gatcttctgt ccaaacttcg attcctaatt cttgtgcttt ggtcagcttg
    30661 ctgccagcat cttctcctgc taccacgata tccgtttttt tcgatacgct accagtcact
    30721 ttcccgccaa gattttcgat cgtctctttt gcttcttcac gagtaaatcg tgttagttta
    30781 ccagtaagga ctactgtttt ttctttaaaa ggagactcga cttcttgcaa ttgggcattt
    30841 cgaagccctt tgtattcgaa attgacgcct gcttgtttta attcattcat caattcatgg
    30901 acttcttcgt tactgaaata agtgactaca ctgtcggcaa tgatatcacc gatcgtatca
    30961 agcgcaataa tcgactcata gtcactttta ctcaatgttt ctagatcacc gaaatgttct
    31021 gctaaaattt ttgctgcttt cgctccgaca tgtcggattc ctaacccgaa aatcaaccgc
    31081 tcgaccgaat tatctttgct gttgtcgatt gctgtcaaaa tattatttgc tgatttttct
    31141 tttattttgt ccaacgtcaa taactcttct tctgtcaatt tgtaaagatc agcaacatct
    31201 gctactagtt ttttatcata catctgttct agtacgcgcg gccccaaccc atcgatattc
    31261 atcgcatttc ttgaaacaaa gtggttcaaa ccttctttca tttgagcagg gcatttagga
    31321 ttgatgcacc gtaaggctac ttcttcatcc aaatggacaa gttcacttcc acaaactgga
    31381 caatgtgtag gcaattggta ttcttcgctg tctttaggtc gtttatctaa cacgacttgc
    31441 gaaacttctg ggatgatgtc tcccgcttta taaatcagaa ctgtatcttt caagcggata
    31501 tccttcaact taatataatc accgttatgc aggctggcac ggcttactgt cgttcctgct
    31561 actcggactg gtgtcatgat agctgttggc gttactaccc ctgttcgacc gactgtccat
    31621 tcgatgtttt cgattagcgt ttcaacttct tctggcggaa acttgtaagc tgctgcccat
    31681 ctcggtgctt tgatagtaaa accaagttga tcttgtagcg agaattcatt cactttgatg
    31741 acgattccat cgatttcata gggcaaatcc acacgtttgt catgatattc ttcaatgtat
    31801 gaccacactt catcaatcga atgacataga cgtttttccc gattcgtgtg gaatccaatt
    31861 ttttctagct cttctagcgc atcatactgg gtttttgctt gcataggacc gaaatcagct
    31921 actgtgtata aaaaagtgtc cagatttctt ttcgctgtga ttttagaatc taactgacgc
    31981 aaagaaccag ctgcggcgtt ccgaggattc gcaaagatat cttttccctc tgcttctctg
    32041 tcttggttca attggacaaa tgaccgtttt ggcatgaaac attcgcctct tacttcgatg
    32101 ttcattggtt cttttaattt gattgggacc gaccggacag tttttaggtt ttcagtgatg
    32161 ttttctccta cagttccatc cccgcgagtc gcacctctca cgaaatttcc atcttcataa
    32221 cgcaaagaaa cggaaagtcc atctattttt agttcacagc aatagtcaac tacacgtccc
    32281 actgcttttt gtacacgttg atcaaaagct atcagctctt ctttgctgaa aacgtcgttc
    32341 aaactgtaaa gcggaatatc atgagtgact ttttcaaaac cttctaatac ttttccgcct
    32401 actcgttgag tgggagaatc agacgtgatc aaatcaggat attgttcctc gattgctact
    32461 aattctgcgt attctttatc ataaacatag tcttccactg ttggtttatc ttctacgtag
    32521 tattcgcgag accattggtt cagtcttgta cgaagttctt ctgctcgttt agcagcttcg
    32581 tcaatcgtca tttcatgttc catcatttac cctccactac taagcttttt caattggcgc
    32641 aaaggcagcc aaaagacgtt ttactccttg acttgggaaa gccacatcta gttctagatc
    32701 cttcgacgtc ccgcttactc ggacgactgt tcctagaccc cattttttgt gtttgacttt
    32761 atctccaggt ttccagctgt tattttcccc tccagtagca cttttagagg gaacaaccgc
    32821 tgtctctggc tgtttatagc tatatgctgt ttttttagcg aatacaggcg tttttttagg
    32881 cgtaggacgc atgccttcga tttccagtaa ctcctgatcg atttcttcta caaaacgaga
    32941 aggtcggtta tattgcgtcc gtccatataa tgttctggag aaagcatttg ttaaatacaa
    33001 cgcttcttct gctcgcgtga tccccacata tgcgagacgg cgttcttcct ccagttcgct
    33061 ttcttccatc aatgctcgag ataaaggaaa aaccccctct tctagaccaa tcaggaatac
    33121 aactggaaat tctaatcctt tggctgcatg caaagtcatc aatgtcactt gggaagcatc
    33181 ttcttctaaa ttgtcaatgt cggaaacaag tgccagatca tttaagaaga ccgttagttt
    33241 ttcttctggt gcatcagcgt cttcttcatt ttgctgttcg aattgtttgt cgaattcttg
    33301 tgtgaccgtt aaaaattcct ccaagttttc gatgcgtgct tgtgcttcta atgtattttg
    33361 gatttttaag tcttctagat atccgctacg atccaaaacc tctttcgtca attctgtcac
    33421 tgtcaagtat gggatcatct gagtgacttc ttggatcatc tctccaaatg cacctaattg
    33481 ctgtcctgct tttccaccga tatttgccag atccacattt tgcgctgctt caagcagtgg
    33541 ccattcatga agagaagcaa aactgcgtag tttttcgata gatcctggtc cgatgcctcg
    33601 tttgggcgaa ttaacgatac gctcaaaact gattgaatct tgaggattta ccagcacatt
    33661 caaatacgct aaaatatctt tgatttcttt acgatcgtag aacttgtgcc ctccaaccat
    33721 tttataagga atatttgctt tcaacagagt ttcttccatt acacgagatt gtgcgttcgt
    33781 ccggtacaag atggcaaagt cgccgtaatt ccgatgattg cttcgaatct cttcttgcat
    33841 ccggtctacg ataaaacggg tttcgtctcg ttcattatct gcacggtagt aagtgatctt
    33901 gtttccttct ttgttttctg tccacaagtt ttttggtttt cgattgctat tgttttcaat
    33961 gacttggttt gctgcgctaa ggatgttttt cgtggagcga tagttttgtt ctagcaaaat
    34021 tactgcagca tctggataat ctttttcaaa atccaagatg ttctgcatat ctgcaccgcg
    34081 ccagccgtaa atactttgat ctgcatctcc cacgacgcac aggttcctaa atctacctgc
    34141 caataaattg actagcgtat actgtgcgtg atttgtatct tggtactcat ctacatgaat
    34201 ataatggaat ttgttttgat aatacgtgag tgaatcagga tgttcttcaa ataatcgaat
    34261 cgtattcatg atcaggtcgt caaagtccat acattgatta tattgaagct ctttttggta
    34321 agcagcataa cattttgccg caatctcttc gtaataactg ccttgcattt ttgcaaaatc
    34381 ctgaggggtc tgcaggctgt tttttgcttg agagattgtt cctaatatac ttcgaggatc
    34441 atattttttc ggatcaatgt tcagttcttt tagtatccgt ttcatcaagg tcagctgttc
    34501 agaagaatcg atgattgtaa agttccggtt atacccgata aaatcaacat ctcttcttaa
    34561 aatacgcaca cacatcgagt gaaaagtcga tacccagaca tcttcgcctc cagatgcgag
    34621 aatcgcattg acacgttctt tcatttcttt tgcagcctta ttggtaaaag taatcgctag
    34681 gatgttccaa ggattgactt ctttttcctc aatcaaatag gcaatgcggt gtgtcagcac
    34741 tctcgtcttt ccacttcctg cacctgccat tagcagtaat ggtccgtcag tgtgaagaac
    34801 agcttctttc tgacgggggt tcatcccgtt taataattca gctttggatg acattaacaa
    34861 catcctttct tttatcaatc ttgttaatta tagcattttc ttggctttca ttaaactagc
    34921 tagttcaaaa tcatttgttt ctttttatgg atttttcctt gtcaattcgt tgatttcgcg
    34981 ctaaattaaa agaaagcgag gcgtttgaat ggaaaaaaaa ggaaaaatca agaaattaga
    35041 taagactcag atatatttta ttctaaaagg aatatttgtt ggactgctga caggaatcgt
    35101 cgttagcctg tttcgattat ctatcgaaaa gctaagcgat ttagttcgaa gtatctatga
    35161 aatgagtcgg gaacagccga tttacttaat tggcctagct atttgctgca tccttgcagc
    35221 atttttcgtg ggttatctgg tcaaaaatga accggatatc aagggaagcg ggattccaca
    35281 agtagaggga cagcttcgcg gggaattatc aatgaactgg ttctctgttt tatggaaaaa
    35341 gttcattggc ggtgtacttt cagttggtgc gggccttttt ctaggtagag aaggaccttc
    35401 tatacagctt ggcgcatcca ttggacaagg agctggacaa ttgtttcgga gtccatcttc
    35461 agaagaaaag attttaattt ctagcggtgc aagtagcgga cttgcagcag cattcaatgc
    35521 tcctatcgca ggattactct ttgttttaga agaaatccat cactcctttt ctccacttgt
    35581 ttggctgaca tcatttgctt cagcaatcac tgctaacttc gtctctcttt actttttcgg
    35641 cttgagacct gttttatatc taggtgacct tccttctttg ccgttgaaat attatggttc
    35701 cttagtgatc ttaggagtac ttctaggcat cttaggtttt atttatcaga atgtcttgct
    35761 ggctttgcca aaattttaca accgattgcc tttaccagct tattttcacg ggttcgttcc
    35821 tttcttgctg attttgccaa ttggttattt atggcctagt actctaggcg gaggcaatca
    35881 aatcgtttta gcttttggac atgcttcctt gccaatcatt atgattatcg gtttgtttgt
    35941 tcttcgattc gttttttcca tgatttctta cggtgggggt cttccagggg ggattttcct
    36001 tccgatattg acattggggg ctttaatcgg tagcttttat ggaaacatta tggttgattt
    36061 cgggatggat ccaatctaca tcaaggattt tgtcgttatt gccatggctg gttatttcac
    36121 agcgattgga aaagcaccgc tgacagcaat tattttagtg acagaaatgg ttggctctct
    36181 taatcatctg atgcctttag gattggctgc tcttgttgct tatatcgtca atgaccttct
    36241 cggcggtaat ccaatttacg aatcattatt ggaacgtcta cttggaaata ctcagaccgc
    36301 ctcactaaaa ggtgcaagga caacttttga ttttccagta acggcagaga gttctttaga
    36361 cggattaatg gtacgagact tcaattggcc taaaaatatg ctgctaatct ctatacgcag
    36421 aggaaatcag gaaatcttga ctcacggtga tacgatcatg aatgtaggag atattttggt
    36481 tatcttgact gatgaagcaa acttgccaag aatcaagcag gaaatcgagg ctaaatcttc
    36541 tatcacaaat ttgaagcaat cattttaacg aacatggaaa aactatttta caacaactat
    36601 tcatctacac tacttgattc tatatgattc tataaaaaaa gaagaggctg ggaaaaccca
    36661 gcctcttctt ttttatttct atgcttctgc tactgcagga gcttttctca ataaaccata
    36721 aatcacacca gatacgactg ctccgatagc aataaacacc aaatacatga ccgggtgact
    36781 cagtaagaat acaacgaaaa tcccgccgtg aggagccagt aatttgattc cgaaagctcc
    36841 gactaatccg ccagttaaag cagaaccaat aataaaactt ggaatggcac gaatcgggtc
    36901 agctgctgca aatggaatcg atccttccgt gacaaacgat agtcccatca cgatgttcgt
    36961 caaaccagca tcttgatctg ttttgctgaa tttattctta aacaaacgtg tagcaacgaa
    37021 aattgccaat ggaggtacca ttcctccagc cataacagaa gccatcacga cacttccgcc
    37081 agttgcaaca gtcgtagcta aagttgctgt accaaacaca taagcggctt tgttgattgg
    37141 accgcctaaa tctgccgcca tcattccagc taataatgca ccaagtaaag cagcattcga
    37201 accactcata cttgtcaaga aaccgttcaa tgcatcattg attgctttca tagggacatt
    37261 gaccagcaac atcaagaaac cagtaatcag taagccaaac actggataaa agaggatcgt
    37321 tttaattcca tccaatgatt ttggtaagtt cttgaataat ttacgtaaaa atacgatgac
    37381 ataaccagct aagaatccac caatcagtgc acctaagaat cctgcaccgc ctgtattagc
    37441 taatgcacca gcagcaaatc caacgatcaa accagggcgg tcaccgatac ttgaagcaat
    37501 aaatcctgct aataccggca gcatgaaacc aaatgcagct tgtccgattt ggttgaacca
    37561 gctagcagct tgattgtaat tccctagatt tgataattga tcatgtggca cgccaataaa
    37621 ttggtcgatc atgaaagaaa gcgcaatagc gattcctcca ccaataacga aaggtaacat
    37681 gtgtgatacg ccgttcatca aatctttgta aattctttgt ccgatagtac catccgcatt
    37741 tccttcttct gaactgcttt cttttccatc accatggaat actggtgcag taccactaag
    37801 ggctagattg atcaactctt ctggtttacg gataccatca ctgacaggac gattgatcag
    37861 ttcttttccg tcaaagcgat tcatctctac ttttttgtct gctgcaacga ttacaccttc
    37921 tgcacgggcg atgtcttcag cagtcaatcg gtttttgatt ccttctgaac cgttcgtttc
    37981 gactttgatc tccacaccca tttctttcgc ttttttcttc aaagcatctt ctgccatgta
    38041 agtgtgagcg atccctgttg gacaagctgt aacggcaaca acgaattttc tttctggatg
    38101 agcagaagct tctgcagctt gtgtttcttg ttctgctttt tcttctgctt ctttttgctc
    38161 ttcagctgtt tggaacaagt cttggacttc ttctggtgta tttgcttctt ttaattttcc
    38221 tacgaaatct gcatcaatca gcaatcgaga tagtgctgct aatgcttgca aatgtgtgtc
    38281 gtttgcgcct tctggagctg cgatcatgaa aaacagataa gttggctgtc cgtctaaggc
    38341 ttcataatct actccttttt ggctttttgc gaataaaact gttgcttctt taactgcact
    38401 gtttttcgcg tgaggcatgg cgattccatc accaagacct gtagaagttt gtgcttcgcg
    38461 ggctagaatt ccttctttat atgtttcgat atcggaaatc cggccggcat caaacatttt
    38521 ttggaccatt tcatcgattg cgcctttttt atcagtcgct tgcaaatcca tgatcatcgc
    38581 ttctttgacc attaaatcct tgatattcat gattatcctc cttgaaacta atcaattttt
    38641 gtgatttcaa cttctggcag caactcatca atcaattcgc gagctgccaa gtcatcagaa
    38701 aaagctgttg cgcttccgca agcaactccc catttaaagg cttcgattgg atctttcgta
    38761 cggctgtaag ccccaataaa gccagcaatc attgaatccc ctgcacctac tgaatttttc
    38821 aatgggcgaa ctaatacatt cgttttataa gttccttgtt ccgtgaagag caaagcaccg
    38881 tctccagcca ttgagatcag cacattctgt gccccttcag ctagtaattt tttcccatac
    38941 ggaatgattt cttctacaga ttggaaggtt gtgttgtaaa gctcagctaa ttcgtgattg
    39001 tttggtttga tcaacaacgg tttcttaaca agtgcatttt ttaggtcatc acctgttgta
    39061 tcaatcacga aatctgcacc tttttctcga atgatatcaa tcagttcttg ataaaatcct
    39121 tttcgcaggc tagctggcgc gcttcctgac aatacgacga tatctccttg tcctactgtt
    39181 cctaatgctt gcttcaactc atcgatttct tcatctgaaa tagatgggcc taagccattg
    39241 atctcagttt cagtgtctga ttttagtttg atattgattc ttgtatcttc ttttacggtt
    39301 gtgaacaaag actggatttg ttctttttca agccacttcg aaataaattc gccagtgaac
    39361 cctcctaaaa accctagtgc tgtagattga gaatcgatcc gtttcaaaat acgggaaaca
    39421 ttgatccctt ttcctccagg taacttgaag tcttccttca ttcggttcaa atcgcctagt
    39481 tttaacccat ctacgcgtac gatataatca attgatggat taagtgttac tgtgtaaatc
    39541 attgtattgc ctccttaatt gttgtctttt cacggaattc attaacgaat tctaatggac
    39601 aacgctcggt tatgatcgtg gctgcttcca aaggtgcaac agaggtgaag gacaactggt
    39661 tgaatttggt atgatcaatc aatacaaatg tctcttcgga atttttcatg gctaaacgtt
    39721 taacagctgc ctcttctgga tccggagtcg tgaatccttg ttcaagatgg gcaccgttca
    39781 ttcccataaa tgctttatta aaatgaaaat tttttagttg ttctatcgca ttagatccta
    39841 aaacagcatt agttgccagc tttatcgttc cacctaaaat aatggtagca agctccaaat
    39901 caactaaatt tgccgcatgt tgaacggaat ttgtcactac tgtgatctgt tttccttgca
    39961 gaaaaggaat catttctaac gtagtagagc cagcgtctaa ataaatgaca tcttcgtcat
    40021 taatggtttc agctgccgat ttggcgattt gctgtttttc gtgaacgttt ttgagtgatt
    40081 tttcactcat attctgctca aaattcaata attgctcttt cttagctccg ccatgaactc
    40141 tttctagaaa cccactattc tccagctctt gtaaatcacg tcttatcgtt gattcagaag
    40201 cattcaaaag gtttacaaga tcttgtgatt tcacgatttt ctgctgttca agcaagtcta
    40261 ggattttttg tctgcgttcc tctgtaagca ttttcacgat cacctcaata caaataatag
    40321 catatttttt ttctactagc aatataaatc agtcaaaaac gttcataaaa gttataaatc
    40381 agtcaaaaaa aaaataaaaa accagtcaga aagactggtt ttttattttt ttgtctatca
    40441 tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt tttcatataa acagaagcag
    40501 gttttctttc cttatgaaga agataataat aaagtcccat cccgccaaac atgaccaaaa
    40561 aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa gagacagacg gataataatg
    40621 atgccggaac cattattcga taatagagtt gtttatccat tttcttcacc tcatattttt
    40681 tgtaaagtaa tcaattgttc taattctcgg taatctaaaa attgcacacg cagatgctgc
    40741 tcacctttct tgacatcgaa ttggatactt cgccgctttt tctttattcg tttgaaggta
    40801 acatcagaga gtctatgttt gatttccttt aacggtaatt ctgacaggga ttctgaagga
    40861 acatagattc ttaaggataa aagatactct tgatattttt catctgttac ctctacttct
    40921 agatattccc taatataata acaataagct tcgattccat tatccaaagc aacagtcgga
    40981 tcgataactt tatagcacat catgcgtgat cgattctcgt caacccaagg aaaatacgcc
    41041 tgagcattta gtgttccgcg aacacgcctt tctttataat ctccttgttg gatatcataa
    41101 ctttttgtta caaaaaatga agcattcttt ttaggaacag gttgcatttg tgcattctga
    41161 tactggacag attggatcgt atggtcagtg taaatgtatt caacgaatgg cggtttcaat
    41221 aaaccgacac cattgtccca agcaagatga tcaaacccaa gctggtccct aaaataggat
    41281 tgaaacattt gattgtttgg cttttgccag ttaaaggggt ctcgataaag ctggatacat
    41341 atgacttcgt tttctaattc cttcactttt tctacttgtt ggatgtcgag aaaaatcggt
    41401 ttagggatga tttgatgata atagcgacta taatacattt cccaacacga agtgaaacat
    41461 aaaccttggt aaaaaagatc gtatcctgga aattggttgc aatcgactac tacttccccg
    41521 ttttgttgtt tcattttagg taaatgttcg atttcatcct ttttctcaaa caatagccgt
    41581 tgagagatct gttttgtatt gtgatagata aactcgctat aagctcgcat ataggcgaac
    41641 accccatgag caatcatttt tttctgaatg aagtctcgaa tcaaaaattg cttttctttg
    41701 aaaacagaga tagacaatac attttttatc aataattgat tatcttccaa gcgaaaacta
    41761 cattcgttct cgtcatttcc tacaactaac caactttcgt ctcttaccga tgctagcatt
    41821 attttttctt gaagctttcg aagatcatac ggatccgctt gttgtgtgaa agagcggata
    41881 aatgttggct ggatcaacag acgtgtcaaa atagcccaat cgcttatttc ctgttttttt
    41941 tcttcttcat ttaagtctgt gagctggata gtcatcaata atttatcttc gccaatcatt
    42001 ttctcacctc tttcaaatct ttttataatt taattataac caatgataaa gaaaaactaa
    42061 aactttatgt cttagaaacc aatttggacg attacatgat agactaagta aaaagaaaca
    42121 aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga taatttagat aaacttttcg
    42181 aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa agaagagatc gatcgatttc
    42241 taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa acaaaagtaa gttaagctca
    42301 aaaaaagact taccagccaa acttttatga aagagtttga ctggtaagtc ttttttattc
    42361 tttttatgat tcaattgctt gttcgattgc tgcccacgct tcttcttttc cctgttttgt
    42421 aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa tccaacgctt ttttgatttc
    42481 gctctcatgc ttgttccatt taccacgagg gatcttatct gccttagtgg caacaacaat
    42541 tacagggata tcataatact tcaaaaattg atacatctgg atatcttctt gagaaggctt
    42601 atgacgcaaa tctactaaag aaacgacagc acgtagctgg tctctagtcg ttaaatatgt
    42661 ctcgatcatc tttccccatt ttgctcgctc agttttggat acttttgcat acccatatcc
    42721 cggaacatcg acaaaatgta atgcgttctc gatcaaataa aaattcaatg tctgggtctt
    42781 tcctggctta ccggaagtac gggctaaatt ctttcgatca atcaatgtgt tgataaaaga
    42841 agatttccct acatttgatc gtcctgccag agctatttct ggcaattgtg tatctggata
    42901 ttgttttggt gccactgcac ttatgataat ttcagcttga tggactttca tggtctctct
    42961 ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa agccaaaatg acttttacgg
    43021 cagcctcttt tcatcaaccc gcttttttct ctgaatcttt atagattact gttggctcac
    43081 cagacaattc agctgcttct tttgtgataa tcactttttc gatgttttca tttgaaggga
    43141 tatcaaacat cacatccatc atgatttctt caatgatgga acgaagtcct cgagcaccgg
    43201 tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc ttcaggttcg aattcaagtt
    43261 cggtattatc tagagaaaga agtttttgat attgtttgac tagcgcattt ttcggctcag
    43321 tcaagatccg taccagatca tctgttgtca atttctctaa agctgccata acaggtaatc
    43381 gtccgataaa ctcagggatc aatccgaatt ttagtaaatc ttcaggaatg atatgctgca
    43441 tgacactttc gtcttcagaa agtttttggt tttgtgtacc aaatccaatc gtcttttcgc
    43501 ccatacgatt cttcacaatc gtttcgatgc catcaaatgc accacctacg atgaacaata
    43561 tgttcgtcgt atcaatctga ataaattctt gatgtggatg tttacgtccg ccctgaggtg
    43621 gcacacttgc aacagttcct tctagaattt tcagcaaggc ttgttggaca ccttcgccgg
    43681 aaacgtcacg agtaatagat acattttcgc ttttacgcgc aattttatcg atctcatcaa
    43741 tatagatgat ccctttttct gcaagttcca cattgtaatc agcagattga agcaatttaa
    43801 gcaaaatgtt ttctacatct tcccccacat aaccagcttc tgtcaaactt gtcgcatctg
    43861 cgattgcaaa aggtacattc aatgtttttg ctaatgtttg tgctaaaaat gttttaccag
    43921 accctgtagg tccgatcaag cagatattgc ttttttgcag ttcaacatca tctgtgtttt
    43981 cttgtgcttg ctgattgaca cgtttatagt gattgtatac ggcaacagct aacgagcgtt
    44041 ttgcgcgatc ttgtccaatc acgtagttat tcaatacttc aagaatttct agtggtttag
    44101 ggacttcagt gaattcgcgt acagcttctt cataaaattc ttcatcaatg atttctttac
    44161 ataggtcgat acattcatta cagatgtaga cccctggacc agctacgatc tttttcactt
    44221 cttcttgggt tttcccgcaa aaagaacagc gtacggtctc attgctactt ggattgtcat
    44281 acatggtctt caccccttaa cttgcaaaaa gtatgcaaga aactatcaaa gactttacag
    44341 tcttttacta tcataacata gtttagtaaa aagaaaaagt gtttaacaaa ggatcaactg
    44401 acctatacct ttgcattttt tactcttaac aaaaaataag gaactgtgac aaaatcgtgt
    44461 cacagtccct tatttcgaat aaacaatttt ctagaagtag cttcttcaga aatgatttat
    44521 tacttgattt ctattctttc gcttctgtcg ctgtacctgt gatcaattcg attgcttttt
    44581 tcattgtcac atcatgttcc agcatatctt ctgtcaacac gcgtttgatt tgttcgattg
    44641 gcatgttgta tgtttctgat aattcttgga tttctgcatt gatttcttct tctgttgctt
    44701 gaagattttc agcagctgca attgcttcga tcacaagatt tgttcttgtg cgcatttctg
    44761 cttcaccttc gaattgttta tgcaagtctt cttctgtaga accagttaat tgatagtaca
    44821 tttcaggaga gataccttga cgttgcatgt tgttcaagaa ttcgtccatt gaacgatgta
    44881 cttcatcatg aaccatcacg tgtggcaatt caacgatttc tgcgttttca acagccatac
    44941 ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc ttttgtttct actaattctt
    45001 tgcgatattt ttctttcaat tcgtctaatg ttgatacttc gtcatcaaca tcttttgcga
    45061 attcgtcatc taattcaggc acttcttttg cttttacttc atggattgtc actttgaaga
    45121 ctgcttcttt tccagcaaga tcttctgctt gataatcttc tgggaaagtt acatttacat
    45181 ctagactttc acctgattta tgaccaacta attgatcttc aaaacctgga atgaatgagc
    45241 ctgaacctaa ttcaagtgag taattttcgc cttttccgcc atcaaacgct tcgccatcta
    45301 caaagccttc gaagtcgatt actactgtat cgccattttc agctgcagca tcttctttga
    45361 taactaattc agcttgtgct tcttgttcac gtttgatacg tgcatccacg tcagcatctg
    45421 ttacttcacg gtcttgtttt tcaacaacta gatctttata ttgtcctaat tttacttctg
    45481 gtttcactgt tacttcagca gtgattaccc agtcttgacc tttttccatg ctttcaacat
    45541 cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt gacagcagct tcataagctt
    45601 ctggtaatac agcattcaat gcatcttcat ataaagcttc ttcaccgtac atacggttga
    45661 atacttgacg agaaactttc cctttacgga atccaggtac gttcaagctt tttttcactt
    45721 tattaaacgc agttgttaat cctttttgga tttcgtcttg cgcaatcgta aattttaaaa
    45781 caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat ttaattccct ccgagtttag
    45841 aaattcattt tatacatcaa aaacgtttca tgcatacctt taaatagtac actaaccctt
    45901 tcaaattgta aacatattga atggatagaa tccttgtttt tcataacatt tttatctttt
    45961 tcactgataa aatctaatgg aagaagatca tttgctgcaa aattttttct ctttttttta
    46021 aaaaatcttg tttttcatct tctgtacctt catacatcgt ttttcctgta cacgcctcta
    46081 cagtcagttg aaaccagcgc ttcgggtctt cgatgtatgt atcatagaca ggataaacaa
    46141 ttgcagattg caacgtgaat tcttctttta acacggaact aagcagcgca tcttcattcc
    46201 cgtaacgatc tgaaaataca cataattgct ggtaacttgc ctgctgctgc ggctttggca
    46261 gatcttttgg tatgactttg actaactttt cttcaatcgt caaatagaaa acaggatggc
    46321 attcgttcaa ttgcctaagt gtctccagta attttgccct tgctaaaata tggacctctg
    46381 gcgatacaat aagtttactc gctgtttgga taaactcatg ataaggaagc tgttcgatct
    46441 catcaataga cgcaagctga cgtgcaggtt ccaacgaagg aagttcctcc gatttattgt
    46501 ggagtgcctt gatcttctgc ctctccattt gtccggataa ttcttctgca aattggattt
    46561 tttcaatgac ccgctgctcg aaagcttcat aaaaatctgt acttgctatc aattttctag
    46621 catataaaaa atcttgtgta ctgattaaca agtcaaaata aaattcagca aactcctcat
    46681 tttgaagata ctgtttttca tgcaagagtg cttgcttcaa tgcttctttt ttctcgtcca
    46741 actcgtaaag acaaaaaaca agcaggctat tagcctcaaa gctttctttc aactcgtaag
    46801 ctcgttgaaa taattctttt gcctgctgat attgctggtt tgctagtgct tgttgtgcca
    46861 ctcgcattaa attctcatat ttttctggaa aatcaatcgg cttcacgcag tgtcctcctc
    46921 ttgctcatat tcagctcatg ttcaatagta gcaaaacaaa aaaagcatga caattaaaaa
    46981 taactgaagc accctctaaa agctagattt tagatctgac ttttagaagg tgctttcaac
    47041 tgttactgct ttttattgct catcagctgt tgacttcctg gttgaacgct ttgatcagat
    47101 tgatcgtaac aacacttgta ttagtgaaaa aatcgcccag atgttgcttt ttctgattaa
    47161 agactgctgg aagataccct aacatcagca aaggaaattt tagaatgaac cggcaagcac
    47221 cttctctaac taaaacggtt gaccagctaa gttcttcttc atcaaagctg atgactcgaa
    47281 taccaaagat cattttccca atcgtttggc catgattgaa tttcgtcaat aaaataaaat
    47341 acgctagata aataagtaag gctgtgaatc cgtagagact cagataagaa tctgaaaaat
    47401 taagccaacc tagatttttg gcaataccaa gtgttaaacg cgtaatagct ccgatacaca
    47461 aaagatcgac caagaaggca aacattcgga taaaaaaacc tgcatagaaa tacttaggaa
    47521 aatcatttgc ttttggcggt tcatttcttg tgtccgtata ctttttccat ttacgttgtt
    47581 tctttttgat ttcttcgggc gtcaaaggct ctctttcaaa agagctcagt accttttggg
    47641 aaaagttgga gtcaggtcct acagcaggct gctgttgctt atctttttct tgttccttat
    47701 tttcatttga aagctctgac atgcttactc acctccgtaa taatacattg cttttggtga
    47761 ttccgctgtc cctagattct caagaacgga taagatctga ctagtctcag aaggcttcaa
    47821 tccctggaat tctgctagtt tacttcctaa ccatgtacta gcaaacccag atggcgtgga
    47881 agaatattct actagttccg catcttcaag ctgttgttcc tttcgtatag cttttaaagc
    47941 atcttcagga aatcccaatt catcgactaa acctacttct tttgcttgga caccatcata
    48001 aatccttcca tcagcaattt ttttgacttc ttcttcagat ttattccgtc cctcgctgac
    48061 gattgctaca aaacgactat atgcgctgtc gatatatgct tgaagcaccg cttcttcttc
    48121 ttttgtctct ggtcgcgttc ctgaacccat atctttcaac gctccgcttt taactgttgt
    48181 atcttccaca cccagttttt ctagaagtcc tgaataattc aatcctgaca tgatcacacc
    48241 aattgagccg gtcactgttt cttctgttgc aaagatttta tctgcttgag cagaaatata
    48301 gtagccaccg ctagctgcca tgtttttcat gctgacatac attgggatat catgttcttt
    48361 acgaatcgta tccaatttct tagcgatttc cgcactttcg tagacaccgc caccaggaga
    48421 atttacttct aaaaagatac ctttgactgt tggatcttct tcaatcgcct tcagttgttc
    48481 cataaaattt ttatgattgt aaccttcaga agcaaataaa cctgaagacc ctcccgcagc
    48541 aatcgttcca tctactgata attttacgat tttttgcgta ctgtctccct tttccaacac
    48601 atttggagct agttcttcat ccccatataa ccaactgttc aatccctcaa tttgatcgtt
    48661 ttcctgaggc ttagtcaaac tagctgaaac taacgaaacc acaaataatc ctgcagcaat
    48721 cagaactgct gcccaacgtc ttttattcat tctttctcct ttctaataca agttttttat
    48781 gacgatcaca gtttctgact gctcagaagt gtcgaaccgc tctgcttttt cttcccgcgc
    48841 atacccggaa aacgactctt ttgttaattc atcttctacc gtaaactcac gagcttgagc
    48901 aacttccaag gcatcaggtg cgatttcttg gatgctatcc cagccagtat ctaaaaatat
    48961 tttcgtatca tctgatacac cagctgatgc ttcgaatttg gacaattgtt tttttaacat
    49021 tcccagtgtc atgatctcaa tttttgaatc catttttact cctcctctat ccgattactt
    49081 tctattgtac cgaactcctg aaaaagatga tagataaaaa cccattattt tttgacataa
    49141 aaaggacatt tgacaaagaa tttgatatat accaatttat tttttattct ttttttgttg
    49201 acaagaacat tcgcaaaagg ttatgatttt aaatgtttcc agataaaaaa caaaaaggag
    49261 gatagttata ccttttagga tagcgccagg tctgtgtaaa cagatgacga ggagagggtt
    49321 tatcgaaaga ttcggcggat ggccctaggg actgcactct acagaaaatc cacaaaaaat
    49381 gtctgcaaag ataaaacaaa aggattttcc tgcagctgga agcattcata cgaattcatt
    49441 atagaaagta ggatgttttt tatgtgcgga attgttggaa tgattggatt aaaaaatgta
    49501 acaccaggat tgattgacgg tctagaaaaa ttagaatacc gtggttatga ttcagctggt
    49561 atctttgtct cagatggcac aactgattat ctggtcaaag cacaaggcag aattcaaaat
    49621 ttgaaaaaca aaataaatat tgatactaca ggaaatatag gtattggtca tacacgatgg
    49681 gcaactcacg ggcaaccttc tgaagaaaac gcccatccac atacttccca aagtggacgc
    49741 ttcgtgctcg ttcacaacgg cgtaatcgaa aactttgaag aacttaaaat tgcttattta
    49801 gcaaatgacc actttattgg tgagaccgat actgagatta tcgctcatct aattgaaact
    49861 ttcgcgaagg atactacaac gcaagaggcg tttttaaaag ctttgcgggt catcaaaggt
    49921 tcttacgctt tcgctttgat tgatcggaca gcacctgatg ttatttacgt cgctaaaaat
    49981 aaaagtccac tattaatcgg actcggcgat ggattcaatg tgattgccag cgatgcaatg
    50041 gctatgcttg ctcatacgaa ggaattcgtt gagattgaag atgaagagat ggttaccgtc
    50101 acatcagaaa aagttattat tcaaaatttt gcaggtgaca tcgtagaacg ttcttcattc
    50161 gaagcacaag tagatgcttc agatatcgag aaaggtacct atccttttta tatgctgaaa
    50221 gaaatcgacg aacagcctat tatcatgcgt cgtatcgctc aaaaatacgt aacagaagac
    50281 aatcgtgtct ctcttgatca aaagctggtc gatacattgt ctgatagcga tcgaatctat
    50341 atcgttgctt gcggaactag ttaccatgcg gggttagcag gtaaacagac tttggaaaaa
    50401 ttgacacaga ttcctgttga ggttcattta gccagtgaat ttggctataa cacacctctt
    50461 ctttcaaaaa aaccattctt tattttcttg agtcaaagtg gcgaaacagc agacagccgc
    50521 caagtgctgg taaaaatcaa ctgtctcggc tacccttcac ttacgattac aaatgttgct
    50581 ggttctactc tttcgcggga agcatccttt acactgctat tgcatgctgg acccgaaatc
    50641 gcagtagcat caacgaaagc ttatactgca caaatagctg tactaacttt attagccaaa
    50701 gctatcggtg ataaaaaaga atttgaaact tctcttgtat tcgatatcac tcatgaattg
    50761 agtttagttg ctaatgcgat ggaatcggtc atcgcccaaa aagattattt agaagagttg
    50821 gcagaagtat atctcagcga tacaagaaat gccttttaca ttggacgtgg tgctgactac
    50881 aacgtttctc ttgaagctgc tttgaaattg aaagaaatca gttatatcca ggcagaaggt
    50941 tttgccgctg gcgaattgaa acatggaacg attgctttga tcgaagaagg aacaccagtg
    51001 atcggaatca tcagtgaaga agtgactggt gcgcatactc gaggaaacct gaaagaagtc
    51061 gaaagccgtg gcgctaaaac actagttatt gtctcagagg gattagaaaa ggaaagtgac
    51121 cagctggtgc taccaactgt ccacccttat ctaactactc taaccacagt cgtaccgact
    51181 caattacttg catattacgc aacgctatta cggggattcg acgttgacaa accacgtaat
    51241 ttagcgaaat cagtgacagt agaataataa aagaacaagg accagtgtca gacatctgac
    51301 actggtcctt gttcttttat tttttatgaa aatatctctt aatccagtcc gatttcttct
    51361 ttgactactg ccgcgatttt atcgacatag taattaactt tctcatcagt aggcgcttct
    51421 gccatcactc gaagtaatgg ttctgtaccg gaaggacgaa ctaggattct tccttctgat
    51481 cccatttcag cttctgcctc ttcaatcact tttttgattg ccggaacatc catcgcaccg
    51541 tttttatcac ttacgcggat attcactaat ttttgcggat agattgttac ctctgctgca
    51601 agctctgata atttcttacc tgtctgtttc atgatattca gtaactgaat tcctgaaagc
    51661 atcccatctc cagttgtgtt aaaatctaag aaaatcatgt gcccagactg ctcgccgcca
    51721 aagttataat cgtttttgcg catttcttct acaacgtaac gatcaccaac ttgtgtaacg
    51781 acatctttca aaccgattcc ttccaccgct ttgtggaatc ctaagttact catgacagtc
    51841 gtaacgatcg tatctttttt caatcgattt ttttctgcca agtatttagc acagataaac
    51901 atgattttat caccgtcgac aatgtttcca agttcatcca ccgcaataat acgatcgcca
    51961 tcgccatcga aagctagtcc agcatctgct cctttttcaa cgaccatctc agctaaacgt
    52021 tctggatggg tagagcctac gccgtcattg atatttaacc cgtctggatt tgtgcccatt
    52081 gtataaaaat ctgtttcaag atcggcaaac aaacgattaa cacttgttga agttgctccg
    52141 tttgctgcat cgacacaaat ggtcaaaccg gaaaggtcac cgttgatcgt ttgtacaagg
    52201 aattgagaat atttcaataa gccttctgga aattcttcta cagttcccaa tccttcagct
    52261 gatggacgag gcaattcatc cacttcagca tctaataatg cttcaatttc tagctcttgg
    52321 tcatctacta gtttaaaacc atctgcaccg aaaaatttga ttccattatc ttgtgctgga
    52381 ttatgagaag cagaaatcat gacccctgcg cttgcttttt gcaaacgtgt cagataagca
    52441 acacctggtg tagagatcac acctagctgg aacacttcga tacccaccga taaaagtcct
    52501 gaaatcagcg cttcttctaa catttgacct gagattcttg tatcccgccc tactaacacg
    52561 cgtgggcgtt ttccttcttc ttcatgctgg cttaggacat aacctccgca ccgtcccagc
    52621 ttaaatgcaa gttctggcgt aagttccttg ttggcttcac cgcgtacgcc gtctgttcca
    52681 aaatatttac ccatagttgt aaaattactc ctttaactta attttctgag gaatttgttc
    52741 tgctcatttg tgttgattcg cttgtttctg atgtttcttc tgtacttgtg gtcgagctgc
    52801 ttgtcccaga ttccgaactg gaagaaatgg ttgttgtcga tggttccgtt cctgaatcag
    52861 cagatgaaat acttctactc gtacttgtat tttcttgtgt ccgactgctc gtcgaagcag
    52921 agctactgct tgacgtttgc aacacgggtg taatctccac actaactgat tcgggcttga
    52981 tacttaaccc ctcttctact ggtatttcaa ttttacgagt tgttcgatga ttgatacctg
    53041 agacattgat cggtacagca atactgtcac cgatcgatgc aagcaaatcg ctagagccgc
    53101 ttacttctgc ttccagtttt gaaaaactga aatcatagct ttctaccccg gcagctggtg
    53161 ttccttgctg tatcccataa agtcgtaccg acttgcttgg cttgatcgaa tcagctctca
    53221 ctgttacttg cactggatca gagacgatac ttaatacttc ccctgctgca ttcagcgcct
    53281 gtattgggac ctcactatta atcccatcat ctgtgaccat cgaagcgtcc acatcagcta
    53341 ctactcgttt gatttctgcc aatgttttgt cacctgtagt gatagagact tttttaggac
    53401 taacggaagt atcatcaagt tcatatccag atggtgtgaa cgttgaagga atcaacgttt
    53461 ccacatcgaa atcttttatt acctttttct caaccgttac tgtaatagtc gatggttcga
    53521 tctttgctgt gacagcactg ctcaaatttt gaatttttag cggaacttca tgtgttcctt
    53581 cgcccagctt agtgatatct gctgtgacgc ggaacatccg tgtatcttcg tttgcctctg
    53641 cattcaactg aactcggttt gcactggaaa gtttgacagt cactgcattt tcatatccat
    53701 ggatatagta gcgctttgaa tcataagaaa tcttgatcgg cacccccgta actgtttgat
    53761 tatacgcttc atttccagaa agcgtatttg gaacggactg gccatttgca ttgaagtaca
    53821 gcgcaatact gaacagcaat gcaataaaac tgtaaagaat gcttgtccgt ttctcttttg
    53881 agatcatttg cgatcccctc ccttagtcat accgtctagg aaagattgaa tcacgttttt
    53941 cttgtttttt gttttctctt cagcaattag ttctctacgc aagattgcca aatattcttg
    54001 ctgggataag ccagccatca gttcattatc taacgtgatg ctgacgtccc cggtttcttc
    54061 agagacaacg atggtaatcg cgtcactgac ttcgcttata ccgacagctg cccggtgtcg
    54121 agtcccgaat tctttaggga tcaacatact ttctgataga ggaagatagg cgcttgctac
    54181 tgcgatcttt ccttctttta caataactgc accgtcatgc agcggtgtat taggaataaa
    54241 gatattgatc agcagctccc cagtgatatc tgcatctaaa gcaatccctg tctcaatata
    54301 ctcatctaat ccagtatgac gttcgatagt gatcaacgca ccgatttttc gcttggacat
    54361 atattggatg gctttgtcaa aagacaagat catcttctca tcttcctgct gttcgctttt
    54421 tgattgtttg aaaagcgaac tgcgtccaag atgttccagt cctctccgga tttccggctg
    54481 aaaaatcacg atagcagcta tcaccccgta cgtaatgact tggttcatca gccaagacaa
    54541 ggtgtgcaga ccaatgattt ccgctacgat tcggataaca ataaaaatag ctacaccttt
    54601 cagaagctga atcgcttttg tgcctcgcag cagttggatc agtttgtata caagatacca
    54661 gacaaccaga atatcaagta tgttcaccaa aaaattcaga gagaacaagt caccggaaaa
    54721 catttgctgc caatagtcca aattaaaaag ttccccgatt tgaaatgaca taaaaagcct
    54781 ctcttctctt ttgagatttc ctctctagta tatcatatgt tttaatttta gaggctgata
    54841 tgctcgtatt tttcttgaga aaaacgcata attttcaaga aaattgcaag tatgcgtttt
    54901 atttttctct gcaataaata tttttaaact tcagagaatt attcattata ttttttctgt
    54961 tatttttcct ttttttcttt tgtagcttgt tccatttttt tacgtcgttc tgctctggca
    55021 gctcttctag cagctcttct ttcttctgct tcaaggtccg tcgttttttc ttttctagtc
    55081 ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt ttctaattct
    55141 cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cgtggacagt cttttcaaat
    55201 aaagcttttg caagatttgg atttgcgtta tctagcgagg aaaagcgaac ttgtttcctc
    55261 atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat tgggttcttg
    55321 tttttttcga ccattgtagg gttatatcga tataatgacc aataaccaga agaaacagct
    55381 tcttttgctt ctttaagcga ttctttcatt ccgccaagca agccatgtgt gatacaaggc
    55441 gtataggcaa taatcagaga aggaccagga tacttttctg cttcttcaaa tgccttgatc
    55501 gtttgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc atatgtcatc
    55561 gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa tttagcgatt
    55621 gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac ttcgttatcc
    55681 aaaacaagga tattcacgtc tgctccgctg gcaagtacat gatcgatccc gccaaaacca
    55741 atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac gaatagatcc
    55801 tttctcttgt atatatcctc cagttcaggg aaaattgatc gttcgctttc gatcgcagct
    55861 tgcagttttg ctgctctttg ctgcgttcct tcactctcaa acatgtgtac taaccagtct
    55921 tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat tgaacttgcc
    55981 aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc agcattatct
    56041 tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt ataaggagaa
    56101 gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat catcattcga
    56161 tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc tgagcaagcg
    56221 cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt ctttcctttg
    56281 actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc ttgttctttc
    56341 tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc ctctacacac
    56401 agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac gccgtctgcg
    56461 cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc ttcatctgct
    56521 aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca catcgtacag
    56581 cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat agaagccgtt
    56641 cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac agagagttga
    56701 tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt gttcctttta
    56761 ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac ttgatgaagc
    56821 agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat tgatttatgt
    56881 ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa aggaatgatg
    56941 tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc caaaccgact
    57001 tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc agccaagtaa
    57061 cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag agtattcaat
    57121 aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag gtaagctggt
    57181 gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat cggcgtatct
    57241 ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata ataaaaatag
    57301 ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt atttgctcca
    57361 actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg ttctgtcaaa
    57421 tccagacttt cctttggttc caaagattga taagtcacat catccacgat ccctatggta
    57481 aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc tgagatttgg
    57541 tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc cgggcgaagt
    57601 tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc tgcccctggt
    57661 tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt ttgcacgaat
    57721 gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt ccgtccgttt
    57781 gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat agaaacaatg
    57841 acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt tcctcgtaat
    57901 cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata ataggggttg
    57961 atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact aacagtcgga
    58021 tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt gatcagagga
    58081 gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt acggaatcca
    58141 tcaaagaaat tcaaaaaagg gacttctcct tcaatagaag caagatgggc aacaggtgct
    58201 aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt ttgtcttgca
    58261 gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac tgcccgacta
    58321 gcaacgtgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt cggtatcatc
    58381 aaaagcagcc cttgtgaagc ggtatatgta gttgtcagtg caccagtttt caatgatcca
    58441 tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac tggttgaccg
    58501 aatacgtttt cttttcctgc tgctgaccat tgatctacta attcagccat ggtcgaactg
    58561 ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata tgcggctgcc
    58621 gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc atattctctc
    58681 ttattatctg ttccattata acgtaaaaca tgccattttt ttcttttatt tataaaaata
    58741 ttttacatgt atctatttga attggtcatc taatttttag acaaacagtc taaatcgttg
    58801 cctttttttg tcagacatgt ataaactata agcgaaagag gtgttttaac atggatatat
    58861 cagtgattga tgcaacgaaa acaaatacac aaaaaggaat cctttatggt tcatcgaatg
    58921 ctcctaaaaa aatggtcgaa ttcatcaact tagcttgtcc gtattgccga caatggtttg
    58981 aagaatctta tgagctatta gaagaagcag tccaaagcgg gcaattgcag cgagtgatca
    59041 aattgttcga taaagaaaaa gaaagcttac agcgagggaa cgtgatgcac cggtatctca
    59101 caatcagcga tggccaaaaa gcgatcaaag agatcaaaca aatattcgat actcaagacg
    59161 agtggaaaca tctttctctc caagaagtag cggattttgc agtagataaa ctaaagctga
    59221 cagaacaaaa agacgaacag ctgagccaag ctgtcatcaa cgaagctgag caagcacata
    59281 ttcgatttgt tccaactgtc atcttgggta aagaaatctt tgatgaatct atttcaatag
    59341 aggaattaaa agaattgatc caaacaaaat aaaaaaataa ggagcgtg
//
LOCUS       DAZKCZ010000006        57735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000006 DAZKCZ010000000
VERSION     DAZKCZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.006"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1603
                     /locus_tag="ACG8KN_000285"
     CDS             86..1603
                     /locus_tag="ACG8KN_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFP9187376.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1575..2864
                     /locus_tag="ACG8KN_000286"
     CDS             1575..2864
                     /locus_tag="ACG8KN_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9187377.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3039..3935
                     /locus_tag="ACG8KN_000287"
     CDS             3039..3935
                     /locus_tag="ACG8KN_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9187378.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3949..4812
                     /locus_tag="ACG8KN_000288"
     CDS             3949..4812
                     /locus_tag="ACG8KN_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9187379.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4818..5381
                     /locus_tag="ACG8KN_000289"
     CDS             4818..5381
                     /locus_tag="ACG8KN_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9187380.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5403..6914
                     /locus_tag="ACG8KN_000290"
     CDS             5403..6914
                     /locus_tag="ACG8KN_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFP9187381.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6926..7885
                     /locus_tag="ACG8KN_000291"
     CDS             6926..7885
                     /locus_tag="ACG8KN_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP9187382.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            7971..9050
                     /locus_tag="ACG8KN_000292"
     CDS             7971..9050
                     /locus_tag="ACG8KN_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9187383.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9302..10702)
                     /locus_tag="ACG8KN_000293"
     CDS             complement(9302..10702)
                     /locus_tag="ACG8KN_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFP9187384.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10920..12581
                     /locus_tag="ACG8KN_000294"
     CDS             10920..12581
                     /locus_tag="ACG8KN_000294"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9187385.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12538..13236
                     /locus_tag="ACG8KN_000295"
     CDS             12538..13236
                     /locus_tag="ACG8KN_000295"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9187386.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13252..14676
                     /gene="araA"
                     /locus_tag="ACG8KN_000296"
     CDS             13252..14676
                     /gene="araA"
                     /locus_tag="ACG8KN_000296"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9187387.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            14983..16142
                     /locus_tag="ACG8KN_000297"
                     /pseudo
     CDS             14983..16142
                     /locus_tag="ACG8KN_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(16119..16259)
                     /locus_tag="ACG8KN_000298"
     CDS             complement(16119..16259)
                     /locus_tag="ACG8KN_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFP9187388.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            16260..18119
                     /locus_tag="ACG8KN_000299"
     CDS             16260..18119
                     /locus_tag="ACG8KN_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9187389.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18229..18663
                     /locus_tag="ACG8KN_000300"
     CDS             18229..18663
                     /locus_tag="ACG8KN_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187390.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(18922..19083)
                     /locus_tag="ACG8KN_000301"
     CDS             complement(18922..19083)
                     /locus_tag="ACG8KN_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187391.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(19102..20124)
                     /locus_tag="ACG8KN_000302"
     CDS             complement(19102..20124)
                     /locus_tag="ACG8KN_000302"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFP9187392.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            20252..20605
                     /locus_tag="ACG8KN_000303"
     CDS             20252..20605
                     /locus_tag="ACG8KN_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9187393.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20617..21783
                     /locus_tag="ACG8KN_000304"
     CDS             20617..21783
                     /locus_tag="ACG8KN_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9187394.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21875..22495)
                     /locus_tag="ACG8KN_000305"
     CDS             complement(21875..22495)
                     /locus_tag="ACG8KN_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9187395.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(22687..22983)
                     /locus_tag="ACG8KN_000306"
     CDS             complement(22687..22983)
                     /locus_tag="ACG8KN_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9187396.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            23187..23837
                     /locus_tag="ACG8KN_000307"
     CDS             23187..23837
                     /locus_tag="ACG8KN_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFP9187397.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            23853..25580
                     /locus_tag="ACG8KN_000308"
     CDS             23853..25580
                     /locus_tag="ACG8KN_000308"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187398.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            25577..27469
                     /locus_tag="ACG8KN_000309"
     CDS             25577..27469
                     /locus_tag="ACG8KN_000309"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187399.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            28173..29042
                     /locus_tag="ACG8KN_000310"
     CDS             28173..29042
                     /locus_tag="ACG8KN_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9187400.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            29251..30276
                     /locus_tag="ACG8KN_000311"
     CDS             29251..30276
                     /locus_tag="ACG8KN_000311"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9187401.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            30315..31265
                     /gene="manA"
                     /locus_tag="ACG8KN_000312"
     CDS             30315..31265
                     /gene="manA"
                     /locus_tag="ACG8KN_000312"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFP9187402.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            31359..33062
                     /locus_tag="ACG8KN_000313"
                     /pseudo
     CDS             31359..33062
                     /locus_tag="ACG8KN_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            33064..35097
                     /locus_tag="ACG8KN_000314"
     CDS             33064..35097
                     /locus_tag="ACG8KN_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFP9187403.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            35108..35548
                     /locus_tag="ACG8KN_000315"
     CDS             35108..35548
                     /locus_tag="ACG8KN_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9187404.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(35748..36875)
                     /locus_tag="ACG8KN_000316"
     CDS             complement(35748..36875)
                     /locus_tag="ACG8KN_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9187405.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(36878..37555)
                     /locus_tag="ACG8KN_000317"
     CDS             complement(36878..37555)
                     /locus_tag="ACG8KN_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9187406.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            37819..39585
                     /locus_tag="ACG8KN_000318"
     CDS             37819..39585
                     /locus_tag="ACG8KN_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFP9187407.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            39600..40037
                     /locus_tag="ACG8KN_000319"
     CDS             39600..40037
                     /locus_tag="ACG8KN_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9187408.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            40050..41201
                     /locus_tag="ACG8KN_000320"
     CDS             40050..41201
                     /locus_tag="ACG8KN_000320"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9187409.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(41263..41961)
                     /locus_tag="ACG8KN_000321"
     CDS             complement(41263..41961)
                     /locus_tag="ACG8KN_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFP9187410.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(41954..43306)
                     /locus_tag="ACG8KN_000322"
     CDS             complement(41954..43306)
                     /locus_tag="ACG8KN_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFP9187411.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(43529..44929)
                     /locus_tag="ACG8KN_000323"
     CDS             complement(43529..44929)
                     /locus_tag="ACG8KN_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9187412.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            45090..45827
                     /locus_tag="ACG8KN_000324"
     CDS             45090..45827
                     /locus_tag="ACG8KN_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFP9187413.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            45952..46431
                     /locus_tag="ACG8KN_000325"
     CDS             45952..46431
                     /locus_tag="ACG8KN_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFP9187414.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            46428..47195
                     /locus_tag="ACG8KN_000326"
     CDS             46428..47195
                     /locus_tag="ACG8KN_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFP9187415.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            47425..47994
                     /gene="lepB"
                     /locus_tag="ACG8KN_000327"
     CDS             47425..47994
                     /gene="lepB"
                     /locus_tag="ACG8KN_000327"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9187416.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(48098..48700)
                     /locus_tag="ACG8KN_000328"
     CDS             complement(48098..48700)
                     /locus_tag="ACG8KN_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9187417.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            48806..49036
                     /locus_tag="ACG8KN_000329"
     CDS             48806..49036
                     /locus_tag="ACG8KN_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187418.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            49027..49275
                     /locus_tag="ACG8KN_000330"
     CDS             49027..49275
                     /locus_tag="ACG8KN_000330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9187419.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            49381..49965
                     /locus_tag="ACG8KN_000331"
     CDS             49381..49965
                     /locus_tag="ACG8KN_000331"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9187420.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            49968..51041
                     /gene="prfA"
                     /locus_tag="ACG8KN_000332"
     CDS             49968..51041
                     /gene="prfA"
                     /locus_tag="ACG8KN_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9187421.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            51034..51873
                     /gene="prmC"
                     /locus_tag="ACG8KN_000333"
     CDS             51034..51873
                     /gene="prmC"
                     /locus_tag="ACG8KN_000333"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9187422.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            51866..52045
                     /locus_tag="ACG8KN_000334"
     CDS             51866..52045
                     /locus_tag="ACG8KN_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187423.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            52067..53092
                     /locus_tag="ACG8KN_000335"
     CDS             52067..53092
                     /locus_tag="ACG8KN_000335"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9187424.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            53267..54511
                     /gene="glyA"
                     /locus_tag="ACG8KN_000336"
     CDS             53267..54511
                     /gene="glyA"
                     /locus_tag="ACG8KN_000336"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9187425.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            54664..55611
                     /locus_tag="ACG8KN_000337"
     CDS             54664..55611
                     /locus_tag="ACG8KN_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFP9187426.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            55745..56374
                     /gene="upp"
                     /locus_tag="ACG8KN_000338"
     CDS             55745..56374
                     /gene="upp"
                     /locus_tag="ACG8KN_000338"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9187427.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(56513..56755)
                     /locus_tag="ACG8KN_000339"
     CDS             complement(56513..56755)
                     /locus_tag="ACG8KN_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187428.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            56876..56948
                     /locus_tag="ACG8KN_000340"
     tRNA            56876..56948
                     /locus_tag="ACG8KN_000340"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56909..56911,aa:Thr,seq:cgt)
     gene            complement(57067..57705)
                     /locus_tag="ACG8KN_000341"
     CDS             complement(57067..57705)
                     /locus_tag="ACG8KN_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFP9187429.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        19186 a        10108 c        11941 g        16500 t
ORIGIN      
        1 aaaaataagt aagtttaaca tttatattat tgacaacgct ttcaacgagg tgtattattc
       61 atgtataaat taaaggaggt cagttatgga agccacaatg ttgattagta gtgtcaagcg
      121 tgtaggcaaa gttgatccgc ggttgtacag ttcatttatt gaacatatgg gacgagcagt
      181 atatgaaggg atttatcagc cagatcatcc atcagctaat aaaagtggat ttcgccaaga
      241 cgtcattcac ttggtaaaag aattgaacat tcccatgatt agatatcctg gtggaaattt
      301 tgtgtcagga tttcgttggg aagacagtat tgggcccata gcaaatagac ctagaaggtt
      361 ggatttagct tggcggacaa ttgaaacgaa tgaagttggg attcatgaat tttataactg
      421 gtgtcagcat gtgggggccg aaatcaatat ggctgtcaat ctgggtactc gtggagtaga
      481 tgcagctcgt aatttagtcg aatattgtaa ttttcctaaa ggaacttatt ggagtgattt
      541 aaggcgttcg aatggtcaag agagcccttt tggaattaag acatggtgtt tagggaatga
      601 aatggacggt ccttggcaga ttggacataa aacagcggaa gaatatggac ggttagcaga
      661 ggaaacggct aaggcgatga aactcgtaga tgattcaatt gaggtggttt tatgcggaag
      721 ttcgaatcgg caaatgccta catttggtga ttgggagtta acagttcttg atcaagcgta
      781 cgatcaaata gattatttgt ctctgcatca atattatggc aaccaaaaaa atgatctttc
      841 tcattatctt gcacgttcct tagacatgga tcggttcatt tctgaagtga cagcgatgtg
      901 cgatacagtc aaggcgaaga aacatagtaa gaagcagatc aacctgtcct ttgatgaatg
      961 gaatatttgg tatcattcag cggaacaaga tgagcaagca gcgccttggc agattgctcc
     1021 tcctttacta gaagataact ataattttga agatgcatta ctattaggct gtctattaat
     1081 cactttattg aaacatgcag atagagtaaa gattgcttgt cttgctcaat tggtaaatgt
     1141 tattgcacca atcaggacag aaacaaacgg agaagcttgg agacagacga tctattatcc
     1201 atttatgcaa gctgctgtgt atggacaagg agaggtgtta agcccacaaa ttcgttcagc
     1261 aaactatgcg acggaagatt ttgaagaagt gccgtattta gaaagtatag ccgtaataaa
     1321 tgacaaagaa cttgtcatat ttgcagttaa ccgtggagaa gaagaaatga atctaactgt
     1381 tcacttgtct gaattatcat tagagggagt gattgatttt tctgaaatga gtggatttga
     1441 tacaaaagca actaatcttc ctaaaagtga acaagtaaaa ccaagtcatt caacaaatgt
     1501 aaaagctgaa gatgggaaaa tcaattgttt attgaaaccg ctatcttgga atgtggtcag
     1561 atgtcgttta tcagatgaag gaggaacaaa aaatgaaagg taaaaaatgg ttaattggct
     1621 ctttgttggt agctgcaaca ggtattttag gagcttgcgg tggcggaagt tcagcagaat
     1681 ctgattctga taaagaagtg actttgactt tttggaatgg gtttactgca tctgatggtg
     1741 aaatattgca agaaatcgtt aatgaattta ataaaacaaa tgataaaaac atcaaaattg
     1801 aaatggatat catgacttgg gccaatttga atgaaaaact tcctacagcg atttcttcta
     1861 aaaaagcacc tgactttatt gcattaaatt atccagattt tgcacagtat gtagaaaacg
     1921 gagcggtaca accattagac gatttctggg aatatgatgg agtagataaa tctgatttta
     1981 aagaaacggc tattgaatta ggtgaagtag aaggtaaaca gtattttgtc cccatgcagg
     2041 tacaaggcat gtatatgtat tggaataatg atttgtttaa acaagctgga ttagatactg
     2101 aacaaccacc tactacttgg gaagaattga cagaaatggc accaaaatta actgatagta
     2161 gtaaaaatgt atcaggtttt gtcttcaata aggatggaac tgcaccgtta tataactgga
     2221 tgttagcaaa tggcggtaaa ttagtgaacg atgattatac taagtctgaa tttgcgtctc
     2281 ctgagaacct taaaacgtta aaagcgatcc aaaaaatgat ccatgtagac aagactggtc
     2341 cagaatcgat ttctggcgcc gaaatggaca atttaatgaa tgctggtcaa ctagccattg
     2401 aaatcaatgg gccatggtta aataacggat tgaaagcaaa cgagattgat tataatgtga
     2461 ctaccttacc acaagggagt gattcagaca aaaaaacagc tattttagat ggcgtaggat
     2521 atgccattcc ggcaagtaca gataataata aaaaagaagc tgtctatgag tttttgaaat
     2581 attggaatac aactgaaatt ggtaaaaaat ggagtatcga aaatggtttt cctgcctact
     2641 tggattcagt agcaaaagat gaagaagtaa aaaatgatcc tattgtatct gaattatcga
     2701 aacaaatgga ctatgcagag ccatatttac caggttttac ccaaatctct gcgatcaata
     2761 acgatatcat caatccactg attgaaaaat tattagcagg agaagatcct gagacactca
     2821 tgaacgatgc agatgatgcc atcaatcaat tactgcaaaa atagtacaga actttataca
     2881 ggatagactg ttggcaagat acagatatct gaatgatcga cctagtattt gcagataggt
     2941 gttattgcaa aaatcacagc gattgttcgg atatcttatt cagattcgtt taaaccaaga
     3001 ttgtatattt tatcaaaatg aaagatgagg tggactatat gaacggattc accaaatatt
     3061 ggaatcgtcc tgatcgtgct gcgtatttgt ttttaactcc atctttttta gttttattat
     3121 tatttactgt cataccgctt ttaggaacat ttgctattag ttttacagat ttgaatattt
     3181 tctttacatc aagaaatttt gtaagttttg aaaatttcac acgtatattt agtgatgaac
     3241 gagcagttaa ttcgttgatt cattctttat atttcaccgt actcgaaacg ccaacacaaa
     3301 ttatcgttgg tttattagca gcagtagcat tatctaaaaa taccagattc aatcgttttt
     3361 gtcgttcgac attttacatt ccagttattt gttcattgac gtcaattgcg attatttttt
     3421 ctatgttgct tgatccgaat gtaggggcga ttccttactt gttttcacaa gtcggcttgc
     3481 cgatccctca attttttcgc gatccaaccc ttgctatgcc gacggtagcc atcatgactg
     3541 tttggaagaa ttttggtgtt actatgacga ttctcttgac tgctattcag ggaatctctc
     3601 cctctttgta tgaatctgct caaatggatg gtgcaacaaa tcgacagcag ttttttaaca
     3661 ttacgctacc tcagatcgtt ccctccttag gtttttgtat tttaacgaat ctgattggtt
     3721 cgatgcaagt gtttgaccaa gtatatgttg caaccgctgg tggcccacaa tttaagacag
     3781 aaacagctgt tcaatatatt tacagtcgag gatttacagc accttatgaa ttgggctatg
     3841 catcagcact gtcttcggtt ttgtttatca ttgttgcaac tatagctatc tccactaatg
     3901 tgtacatgtc acggaaagaa aaacaaatga aataggaggg aaacctgtat ggcaccagaa
     3961 agctcgatta cagttactgt gaatagtcga aaagcagtta gtggtaaaaa aatcattcga
     4021 acgattagta tgctagttat attgttggtt attgcgtttc cttttttgtg gctgattatt
     4081 tcttctttta aacacgaaaa agacattatc tcatttccgc ctagaatatt tgcagatagc
     4141 tataccctag ataactatat taaggtttgg acaacgatcc ctttattaga ttacatcaaa
     4201 aatacagtga tttttgctgg agggacagtg atcacttctg tattctttga ttctcttgcg
     4261 gggtatgcat ttgctcgtat gcgtttcaaa ggaaaatcag ttttgttcta tttcgttttg
     4321 ctgacaatga tgatcccatt ccaagtcttc atgattcctc tatttattga agtcaatttg
     4381 cttggcatgc ttgatactta tgcaggtttg atcattcctc gtatgacaac tgcatttggt
     4441 atcttcatga tgcgttcctt ttttattact ttaccagatt ctttggaaga ggcagcacgc
     4501 attgatggat taagtgagtt taatattttt ttgaaaatca tgttgccatt gagcaaacct
     4561 actcttttat cattagggat tttcacgctg atgaatagtt ggaatgactt gctttatcca
     4621 ttgatattga caagtagttc aaaaatgaga actttaccag cagggttagc tttatttaca
     4681 gggcagaaca tttcttttta tggacctgta atggctggaa cggttatttc aatgctgcca
     4741 ttactagtgg tttatatttt tgcacagaaa tattttgttc aaggaacagc tatgtctgga
     4801 atgaaagagt gaggaagatg aaaatagttg agactaaact ttatggacag ttgattgtgg
     4861 tttctgatta catcttatta ggtattctct gggtaatcgt gtgtttgcct gttattacta
     4921 tcgtgcctgc tacatgtgct gttttttatg taatggcgca atggaaaggt gagggatcag
     4981 gaaatatcat ttattttttc tttcaaggaa taaagcaaca ttttggtaag aacttgttcc
     5041 ttagtctgtt aacgatcatt atttattttc ttgttaatca attattagaa gaacagacaa
     5101 tcgtactttc aattagtgga tatgtagttt tacttattta ttccatgttt cttttatcct
     5161 ggatatatgt gatttctgaa aacaaagata tcacattttt acaaattttt gaacaaagtg
     5221 catggaaagt attttttgat tttttgagaa atattctttg ttgcgtattg ttattaggat
     5281 ttgtactgct catatttctt ttccctcctt ttatttttat ctttgctggt ggcgtatgga
     5341 aacttgtatc tattatcttg actataggaa atgaaaggta gggaaagctg ttggacgaag
     5401 aagtgaaagt aaaaatcaga gaggatttta cggtaggaga gatcagttct cggctgtttg
     5461 gctcatttgt tgagcatatg ggaagtgtcg tctatacagg gatttatgaa ccgggccatg
     5521 aaacagctga tggtgacggg tttagacaag atgtcctctc ttatgtaaag gaattagaat
     5581 taggtgtcat tcgctatcct ggaggaaact ttacatctgg ttatgattgg gtggatactg
     5641 taggtaggaa agaacaacga ccaaaaaaaa ttgatttagc atggcgaggt atagaaccaa
     5701 attcttttgg actccacgaa ttttttaaat ggatcagtca tgtacaagcg aaaccgatat
     5761 tcacggtgaa tttaggaaca aaaggaattg atgaagcaaa aaatttagta gaatattgta
     5821 attttccaaa aggaaccttc tggagtgatt taagaataaa aaatgggcaa aaaaagcctt
     5881 ttggcgttaa attatggtgt ttagggaatg agctagacgg tccatggcaa gtgggggcta
     5941 agagtgctta tgaatatggt aggttagcaa atgaagcctc aaaagtcatg aagttagtgg
     6001 atgattcaat tgaaacaatc ttagttggaa gctctacgcc acgtttacca tcgtaccctg
     6061 aatgggatcg tatcgcatta gaagaagctt atgagaatgt agattattta gctttgcata
     6121 actatattga taagtatgat gaagaagact tgactaaacc tcctaaaaat gagcgtgatg
     6181 atacagcgac ataccttgca aaagcttatc gattcgatcg tcaaattgaa gaaatcgttg
     6241 ctacttgtga ttatgtaaaa ggatatttga gaagtgagaa aacaatgtat ctagcgtttg
     6301 atgaatggaa tgtacatgcc caaccggaaa aaagacatag ggattttgaa gtcggtagtc
     6361 ccatcgattg gtgttacttt actatggaag atacattatt atttggttca ttagggctgg
     6421 ctattttacg acacgcagat cgcgtaaaga ttagttgtca atcattgtta gtaaacacaa
     6481 tacctttaat actgacagat gctaaaggac aagcatggag gaatccaact tattatgtga
     6541 tgcaacactt atcgaaatat gctaaaggtc aagtattgaa acaacaaatc atttgccctg
     6601 tttatgattg tgaaaagata aaaaatgttc cttatgtgga cagtgtgatc gtagataacg
     6661 gcaatcagct gactattttc ttgatcaatc gcacaaatga acaacaaaag ataacgattg
     6721 aacatgattt caatttgcta gatcaaggag agcactacac attaaccagt agcgatctgt
     6781 tggacaaaaa tacacgtgaa gaaccagaaa aaataaaacc tgaaataaac tatttttcaa
     6841 tagaaaaaca tgacgaatta aatgtaggga tagaaaaata ctcttggaat gtaattagaa
     6901 taaagaaaat gtgaggaaat ttgttatgat gaagacactt tataataatc ctatcgttcg
     6961 gcaacgggca gatccttggg tatataaaca tacagatggt tattattatt ttacaggatc
     7021 ggttccaggc taccaagtga ttgaattaag aagggcaaag tcactgaatg aattagaaca
     7081 tgctgcaagc ctgattgtat ggcaggctca tgaagaagga ccgatgagtg aattgatctg
     7141 ggcacctgaa gtccattata tcaatggaaa atggtatatt tactttgctg caagcaatca
     7201 taaaactata cgtgatgaat ctcatcatca ccgcatgttc gtacttgaaa atcagcatat
     7261 agatccaatg aatacagatt ggatagaaaa gggacaaata aaaacgaaaa gggaaacttt
     7321 cagtttagac ggaacaactt tctcttctca aggaaagcta tattatgtgt gggcccaaca
     7381 agatcctgaa attactggga attctaatct ttatctatcc gaaatggata atccttggac
     7441 gttaaagggg aagcagatcc tcctttctat acctgagtat gaatgggaaa aacggggttt
     7501 ttcagtaaat gaaggtccag cagtccttac tcgcaatggg aaaatattca ttacgtattc
     7561 tggtagtgca acagatgaaa actatgccat gggcctgctg tgggctgatg agcacaagaa
     7621 tcttttatta aaagaatcat ggcataaatt atcggagcca gtattcgttt cttctgaaaa
     7681 aaataagcaa tttggtcctg gtcacaactc gtttacggtt tcagaagacg ggtgctttga
     7741 tattttgatc tatcatgctc gaccagagaa gaaccaaaaa ggggatccgt tagacaatcc
     7801 taatcggcac gcgaacgcgc aatgctttac gtggaatgag caaggatttc cagaatttgg
     7861 tgaacctgtt ccgtatgcct tatgagcaca agaaaccttg atttttgctt gaataaacct
     7921 tgcagactcg ttatgatata tgtacgtaca aataaaaagg ggcaaggcaa atggcaaaat
     7981 atcaagatat tgcagaagag ataaaacaaa agatccttcg tgaggaatac gaagtaaata
     8041 caaccattcc ctcagaattc aagctgcaag aaatatatga cgtcagcaga catacgattc
     8101 ggcaagcaat cgctgtgttg gtaaatgagg gctatctgcg taaagaaaaa gggtcgggca
     8161 catatgtaga tgacagctat aagaaagaaa aaacaccatt gaacagaaca aaaacgatcg
     8221 gtgtcatcac cacatatctc tctgattata tttttccgtc gattatccga ggaatcgaaa
     8281 atgaattacg agaatctggg tattcattgc ttttagccag tacgaataac gatcacgaat
     8341 tggagaaaga gtgtttgaag aaaatgattg cttatggggt agatggtttg atcgttgagc
     8401 ctacgaaaag taatcagtat aacccaaact tagcacttta tgttgctttg agagaacaag
     8461 gcattccgat ggtgatgatc aatgctgctt atgaagagtt aaatgcctct tatatttgtt
     8521 tagatgatgt gaaagtcggt tttatggcag cagaatattt gataaaaaaa ggacatcagc
     8581 gtctgttgtt catcacaaaa atcgacgatc ttcaaggaaa atacaggatg aaaggtttta
     8641 tcagcgcgtg tgaacagtat aatgtgcgtt ttttgacgga agatatcatt actttcacca
     8701 cggaaacacg agaaacaatc gctgaaaaaa cagtacaacg gttgaaagaa gaacaagctt
     8761 tagatggcat catttgctat aacgatcaaa tagctagttt aatgctagat aagttgacag
     8821 aaaatggcta tcatattcca gatgacctat cgatcgtagg aacagacgat tcttctttaa
     8881 gccatttagg gaataaaaaa ttgacaacat tgacgcatcc aaaagaaaag ttgggacaag
     8941 atgccgcacg gtggatcgtc acaacgattg aaaaaggaca ggcacagcca aatattctct
     9001 atgaacctgt actagttgaa cgggaaacag tgaaagaaaa cgaaaaataa gagacgggtt
     9061 tttaccgcct cttattgctc ctacggttac tcgtcgcaat gtttgtgaga gcttttgtca
     9121 tcgctcaagg ctgaacgttt ctttcaccac ctctccacta aacggtgttc tcctaccaac
     9181 tggtcacaag tcacaatatt catcaaacat gagaagctgt tttttgaaat tgttccttgt
     9241 gactggaaac taagcggttc cttcttattt cttgaagctg accacttctg tcacaacctc
     9301 tttaagaatg atggatagaa atacgtttag gataagtttt tttagatgca ctttgttcga
     9361 tttcctctaa agatttccca cgagtttctg gtacgatctc ttgaataaat aaaaccccta
     9421 aaaagcagat gatcccaaaa atgccgaaaa cgatttcttg agacatagaa gcagtcatca
     9481 caggaaaaag caagccaact aggaaagaac caatccaatt aaaagaagag gctaggccag
     9541 atgctcgccc gcggacagct agtgggaata tttcgcctac gattacccat gtcagtggag
     9601 cccatgtgca agaataaaat gcgacataaa tacataaaaa caagagaatc aacaacgaat
     9661 ttgtatgagc atccagtaca gttcctaaaa cagctggtag gatgaaagaa agtcccatca
     9721 cgctaccgcc tattttcaat aaagttcgcc gattgaactt gtctgcaata accaaaaaga
     9781 gcaaagaacc agcaactaaa atgattcctt ggatgatcgg ccacatcaat gcatcgctcg
     9841 ctgcatgccc agtcgctttt tctacgatca gcggaatata gtaaaaaata gcattagctc
     9901 cttgaaattg ctggaatact gctacaccaa taccggcaat gactaaggaa cgatattttt
     9961 ctaaaaacaa agtatgccaa gaagttccgg ttgtcttttc ttgtttgaca ttcagctgga
    10021 tttgttcgaa ttcctgttca gcttcttttg gtgtccgaat atagttcaat actctttttg
    10081 cctcttccaa gcggccagat tgcataagaa aacgcggtga ctctggtaga gctaaaacac
    10141 cgaaaaataa aatcagcgcg gggacagcag cgagccctaa catcacgcgc caagccatcg
    10201 tttcaggcaa cccttttagc aaataatctg caacatagga aaggagcatg ccagaagcaa
    10261 tcatcacttg attgatccct gacaatcttc cccttagtct agcaggagcc atttcagaca
    10321 tataagcagg aactagggca gatgcagcgc caacagctaa acctagaaga atccttgctg
    10381 caatcagaaa ataagaacca ttgtgagggg cagatgcaga aaggacagaa ccagccataa
    10441 aaataagtgc agaaagaaga atcatttttc gtcgtccaag cttatcagaa agaagaccag
    10501 ataaagagcc gccaaggatc gcacctaaca tgaccgaaga cgtgatccat cctatcaaag
    10561 aagctttacc tgccaaaccc caatcatgct gtaaaaaagg taaggcgccg gtcataacac
    10621 caatatcata tccaaataga atgccgccaa atgcgccaaa aaaataaata aacgatgagg
    10681 aaactttttt ttcttttttc ataagtattc tctccttcta gaatttcgag aaggcggttg
    10741 atcagatttt gtaagcaatt tcattttata acttatgtac gtacaaaaca agtgtttttt
    10801 attgtattct tgtaaataac tgcttgacat gtacgtacaa aagtatacaa taatcattgt
    10861 aagcgctact aaaaagacgg tttgatcagg tttgacgaga aggagggagt taaatcatta
    10921 tgaaggaagt aaatgcgcaa agtatgttag atgtcataga tggtcgtaca tctttaggga
    10981 ttgagtttgg atcaactagg atcaaagctg tattgattga ttcatctttt actcctattg
    11041 ccgctggtag ttatgagtgg gaaaacaaac tagaaaatgg tatctggacc tattcattag
    11101 aagatatttg gaaaggactg cgcgtcagtt accgaaaaat ggcagcagaa gtattcgaaa
    11161 tctatggcga gaatcttacg acaatcgggt cgattgggtt cagtgcaatg atgcacggtt
    11221 atctagcatt tgatagcaag gaacagttac tagtaccatt tcgtacttgg cgaaatacaa
    11281 tcactgaaga agcagaacga atattaacgg aaaaatttca atacaatatt ccgcagagat
    11341 ggagtatcgc tcatttgtat caatctattt tgaataaaga agaacatgtg aaagaaatta
    11401 attttttgac aacgcttgca ggttatgtcc attggcagct tactggtgag aaagtactag
    11461 gcgtaggaga tgcatcaggc atgtttccta tcgatccttc aacgaaaacc taccatcaag
    11521 aaatgaaaaa tatttttggt cagttaatcg aaaaagcgca cgtagcgatt gacttggatc
    11581 acattttgcc taaagttcta gtagcaggag aagaagctgg caagttaagc gaatcagggg
    11641 cttatctgat cgaccctacg ggaaatttga gaccaggaat cccggtttgt ccgccagaag
    11701 gggatgcagg aaccggtatg gtcgctacga acagtgtagc caaagggaca gggaacattt
    11761 cggctggtac ttccgccttc gctatgatcg tattagaaaa agaactagaa aatgtccatc
    11821 cggaaatcga catggtcact acaccttcag gagaactagt aggcatggtt catacgaata
    11881 actgctcttc ggatatcaat gcttgggtca gtatttttga agaatttacc aattctttgg
    11941 gtatggagat aagcagagat aaattattca cggtattatt caacaaagcg ctagaaggcg
    12001 atacggattg cgggggatta ctttcttatg gttatttttc cggtgaaaac attacaggag
    12061 taaatgaagg aaggccatta tttgtacgta caccaggtag ccgttttgat ttagcgaatt
    12121 tcatgcggat ccatttagcc agtgcttttg gtgcaatgag aatcggaatg gatatattga
    12181 aatctgagaa tgtccaaatt gatcgtttag tggggcatgg ggggattttt aaaacaccag
    12241 aagttggaca gcggattcta gcatctgcta tggaggcacc agttacagtg atggacacag
    12301 ccggagaagg tggagcgtgg ggaatcgcgt tattagcagc ctatatgatg gatcgacatg
    12361 ggaagtcttt agaaaacttc ttggcagaag acgtgtttgg acaagaccaa ggaatcacca
    12421 tcacaccttc agcagaagaa atagcaggct atcagatatt tatgagacgt taccgtgagg
    12481 ggatcgacat tgaaaaaaca gcgattcatc aattgcaaga aaaaggagtg atagaaaatg
    12541 ctggaacaac tgaaagaaga agtataccaa gcaaatcttg acttgcctaa acacggactc
    12601 gtcaaatata catggggaaa tgtgagcgct tttgatcctg aaacacgata tttcgttatc
    12661 aaacctagcg gagtcagtta tgaagagatg acagcagacg acatggtcgt tgtggacttg
    12721 gacaatcata tcattgaagg taaactcaat ccctcttcgg atacgcctac tcatgctgtt
    12781 ttatatcgtt catttcctca aatcggagga atcgtccata ctcactcgac atgggcgacg
    12841 atctgggcac aagcaggatt ggatgttcct gcgatgggga cgacgcatgc agatactttt
    12901 tatggatcgg taccttgtgc tcgatttttg acacaacagg aaatcgacag cgggtacgaa
    12961 tatgaaacag gaaaagttat catagaaaca ttcaaagaac gcaaaattga tcccttagca
    13021 attccagggg tacttttaca cggacacggt ccatttactt gggggaaaga tgcgcaaagt
    13081 gcggtaatga acgcagtcgt actagatgaa gtatgcaaga tgaatttatt taccagacag
    13141 ctcaattcat tttcagaaga gctgcctcaa cggattttag ataaacatta tttacgaaaa
    13201 cacggaaaaa atgcgtatta cggacaaaac aaataaaagg agtggctaat tatgttgaat
    13261 attggagaaa aagaattttg gtttgttgta ggatcacagc atttatatgg ggaagaagca
    13321 ctgcgagaag taaagaaaca tgcacaagaa atggtcgatg aactgaatga aaatggccaa
    13381 ttgccttatc caatccgcct tcaagaatta gcagttactg ctgatacgat cacaaaaatc
    13441 atgaaagagg tcaattatcg agaagaagta gcaggtgtga tcacttggat gcatactttc
    13501 tcaccagcaa aaatgtggat ccgcgggaca aagcttctac aaaaaccatt gcttcattta
    13561 gctacgcaat ataacgaaag cattccttgg aaaacgatcg atatggattt catgaattta
    13621 aatcaatccg ctcatggtga ccgcgaatat gggttcatca atgctcgatt aaataaacaa
    13681 aataaaatcg tcgttggcta ctggaaacga cctgagatcc aaaaagagat tgctgattgg
    13741 atggatgttg cagtagccta taatgaaagt tttggaataa aagtggctcg ttttggtgac
    13801 aacatgagaa acgtgggtgt cacagaagga gacaaagtgg aagcacagat tcaatttggt
    13861 tggactgttg attattttgg tatcggagat cttgtacaag taatcgatcg cgtatcagac
    13921 gaagaagtcg atcaattatt tgaagagtac aaggaattgt ataccttcga ctatggtgat
    13981 tatgaagaaa agacatggga ggaacatgta aaagtacaag ctcagcagga aatcggtatt
    14041 cgccgtttct tagaagaagg aggctataac gcttttacga ctaattttga agacctgtat
    14101 ggaatgaaac aactacctgg actagctgta caacgtttga tggcagaagg atacggtttt
    14161 gctggggaag gcgactggaa aactgcagcg attgatcggt tgctgaaaat catggctcga
    14221 gggaaagata caggattcat ggaagattat acttacgagc tagctagtgg ccaagaagca
    14281 atcttggaat cccatatgat ggaagtagat ccgacgcttg ctgcgacgaa accaagaatc
    14341 gttgtttctc ctttatcaat gggagataga gaagatccgg cgcgtcttgt atttgatggg
    14401 aaagcaggag aaggggtagt cgtatcgatg gcagatttcg gtacgcatta taaactgctg
    14461 atcaatgaag tagaagcctt tgaaccaacg actgaagcac ctaatttacc tgttgcacgt
    14521 gtgttatgga aaactaaacc gaatttccat gaaggtgtcc attcatggat tcaggcaggt
    14581 ggcggtcatc ataccgttgt ctcgctaaat ctaacaacag atcaaattga aacgtgggca
    14641 aaattagttg agttggaaac agtggttatt agataaaaag aggttgtgaa aaagccactt
    14701 agttttaaga aataagaaga gattgacgaa atttgctttt caactttttt gaaatttagt
    14761 cttattatca atgtcagcta acggaacacc atttatcaag atatctcacc ctcaatattt
    14821 tgctctcgaa aataaggctt atggcagtaa cttgtcatag ccttattttt atcactcagc
    14881 ctactctgct ctaactgctc tttttgcttc taggaagtca aatggtaaac agttgtctaa
    14941 ttgcagaaat ttcgagatac ttgttagtaa ggagggggtt ttatgtatca cgttcatttg
    15001 attgatgcta ttccagaaca agagatagaa aagttacaag gggaatattt caaaataata
    15061 gatgatttgc cagccgaagg aatagttatt cgtagtacga aagtgaaaga tgaatggctt
    15121 acaccagatt tgctagcaat ttcaagagct ggtgttgggg tgaacacgat caacgttgaa
    15181 aaagcttctg aaaacgggac gatcgtcatg aatacaccag gtgtaaatgc taatgcagta
    15241 aaagagcttg ttttgtgctg tttgctctta tcttcccgtc cgataatcga agcaagtcgt
    15301 atggtacaaa cactaacagg tcctaatatt ttagaacagg cagaaaacaa aagaagtgct
    15361 tatgtgggac gagaattgca aggcaaaacc atcggtcttc ttggtcttgg agcgataggc
    15421 acaaaagtag cgctaagttg ctatagctta gggatggatg tcttgggtta ttctatccgc
    15481 gatgcccagt tggattatgt tcggcaagca gatttagaaa cagttctttc tacctcggat
    15541 tacatcgtag tcatgctgcc gttgacagaa gatacaaaag gattgatcga tcaggcgaat
    15601 atagaaaaaa tgaaaaaaga tgctgtactt atcaatgtag gaagaagtga aattgttgat
    15661 aaatatgctg ttatgcaagc attggaaaaa caacatctgg ctaaatactt gactgatttt
    15721 ccagaagaag agttcttaga aaatgaccgt atacttatgt tgcctcatct aggggatcga
    15781 ctcaggaagc tttggcagac agcggacgtt tggcagttga agccttaaaa gattatcttt
    15841 tgttcggtac ggttagagaa gcggtcaatt atccatccgc acgaatgttt ttccaagcgc
    15901 cgtttcgatt cactatattt tatcaaaaga aaaccaatat attagcagag atattttctc
    15961 tgctgaatga gaacgagcta gcgatcgcag atatatcgcg taaccataag aatgagcatg
    16021 tctacatatt gattgacata gacagtactg attttgaaca gctcaatcaa gtgaaacaac
    16081 agctagaaaa aatttctgga gtacgaaagg tccgcttatt aaagaaacca gaaaggccat
    16141 aaagaaagag aatatttgga tttcattaat tcttctttcc gttctttact ttcgaatttc
    16201 gtacaactaa aataagagaa gcaacattct tttattgata gaaatgaagg agcaagcatt
    16261 tgaaaagata tataagcaga ctttcgacca aacagaaaaa gattttaaaa ggaacagcag
    16321 caatcatcag tatctttttt cttgttgttt ttagtaattt atttttgcag tggtgtcaaa
    16381 atgatctatc cgtagacctt gcgttgaaat ttgctttttc ttggcatacg gaaaaattct
    16441 tcttagcctg tctcgtcttg ctgattattc ttatttttct gattgcacta gcaggatctg
    16501 tccctttagg ttcccttacg tatgtcgtag ccatcggtgt attaggtttt gcaaattata
    16561 tgaaaatgag ttatcggcaa gaaccgattt atccagacga tttgaagatg atcactgaaa
    16621 tcggattgct gaaagatatg accggcacta tgctgtttac agtgatctta gctgccgcag
    16681 gaaccgtgat aggattgttt tgctggtaca tgtttcgaag cctgaaaaaa ggaaggcgat
    16741 tccaattgat ccgattaacg acactgcttg tggcaattgg actgctaggg tatatcagta
    16801 atttcaacaa tcccgataat ttgttgcgaa aagcatataa taaaacggca ctatggatac
    16861 cttacagcca gaaaatgaac tactataaca ctggttttat cggtgggttt ttgtacaatc
    16921 tgaaggtaga accaatggat gaacctgaag gatattcgaa agaaaagatc aaagaaatca
    16981 ctgaaaaata ccagaagcta gccgatgaaa aaaataaaac tgtagaggaa gagtcgccga
    17041 acatcgtgtt cgtgatgagt gaaagtttct cagatcctag tcgattaaac ggcgtagaag
    17101 tatctggtga gccattggca gattattatg aagtagctga tcaaacatat agtggcaata
    17161 tgctttctca aaactatggc ggtggaacag caaatatcga atttgaagca ttgactggtt
    17221 tttctatggc gttatttaat gcgcaactca ccacccctta cacgatgctt gtaccaaaaa
    17281 tggatcagct tccttctatc gtttcagcgt taaacgcaca atcataccag acgacagcga
    17341 tccatccata caatacttcg atgtataaaa gagaggatgt ttatcagaca ttaggatttg
    17401 accagttcat cagcgagcgt acgatgacct atacggatac gatagagaat aatccatata
    17461 tttctgatga gtcagcttac aaagaagtaa tgacacttct caaggaagaa aaaacacctc
    17521 agttcattca tttagtcacg atgcagacac atatgcccta cgatggaaag tatgataatc
    17581 tagactatcc tgcaaaaact tcagatggaa gtgggacact tgacttagag aattatctgc
    17641 aagatatttc ctatagcagc acggcactga agcagttcac ggaagaattg aagaatcttt
    17701 ctagaaggac tttagttgtt ttttgggggg atcatcttcc agggatctat tcagatacga
    17761 tccaagcaaa aaatgacaaa caaacattgc atgaaacaca atttcttatg ttcgacagta
    17821 aaggagaatt agaaaagcaa acgactcacg atgcaatcac cagtccgttt tattttgcag
    17881 cgaatctaat ggaacagacg aatcagacaa caaatggctt ttatcagctg cttttatcat
    17941 tagaacagga acttccggcg tttgaacgag aactgtacta tcaaaacgga cagtggtata
    18001 aagaagcgca attcaatcgc tcgcaacaag agatttacga cgaatatcaa ttgatccaat
    18061 atgacattgt cgcagggaaa caatatagtt tagcagatgg tttctttgag cacgaataag
    18121 aatggcgagc tgtatagaaa ccattaaatt ttttcaagta cttgtcattt gaggataaaa
    18181 acaagtagaa taaataataa caacaatgga gaaagtagga aaacgaacat gttaccgaat
    18241 attggctatt atgtgcagtc catcgatcaa atcgtcaagg aaacagaagc aatcggagaa
    18301 ggaatgcatc cgaaatatga agtgatcaga aacgcaattg atgaaggaaa aacaagtgaa
    18361 ctggacaaat cgactcttga agagatcatc ctgttatttg aagaaggaac aaaaaaatat
    18421 cgggcaatgc ttatgaaagt cagtactttg cgtccaccag caaaagcaat gggcattcat
    18481 aaaaattttg aaaaagctta tctttcatat gtagcaggct gtgaagaaat gatccaaagt
    18541 atcgatcttg aatcaggggt agatgaagat ctattcaata catctgaagc aaaacaagac
    18601 caagcgacag accaattgac agctgccatc caaaaaatga ctaatttgtt gttgaaaaaa
    18661 taaaagaaat ctttgataac aataaaaact agagttatct aatataaatg taatattacc
    18721 tttatgatag agtgatcaaa atcctttatg ataatgacat acctataacc aacaacaacc
    18781 ttgtacttac tcaaaatcga gtaaatactc cttttccgtc gagtctaacc atctcggcgg
    18841 ttttttattt gtagaaaaga acatcaggat agacggaaaa gtcttgtcct gatgttcttt
    18901 tcagatagta tggaaagctg attagttttc tacattctgt acatattgtt caaagtagtt
    18961 ctgtaaatcg gctttgagtt tctgatattc ctcttcgcta tactctttat cttcgatttt
    19021 cccatcaaag aaaggaatat ttagttcttc ccgtagtctg tctaatacgt cttgctgatt
    19081 catttcattc tccttttctt actaacgatc aaaataaaca cagacacatt ctggtacata
    19141 gacatctttt ttcagtaatt ccagtaatcc tgtttcagga tgacggcgat ataatgtcag
    19201 attatcggtg ttctgattgg cacacacgac aaaattattg cttggatcta gtgcgaaatc
    19261 gcggggaaaa tccccttctg ttgagatgat ttgcttgttt tgaatgtggg aaccatcttc
    19321 aaaaactgcg aatacagcaa tcgagttatg tcctcggtta gaagcataaa ggaacttgcc
    19381 atcatttgaa atgcggatag ctgcccctcc attttcaccg tcgaattctt ctggcaaggt
    19441 cgatattttc tgtaattctt caaaagcacc tgtttcttta ttataagaaa gcacgcggac
    19501 tgtgctgtct aattcaccaa ataaataagc gaattgtcca tttggatgaa agacaagatg
    19561 gcgagggcct gttcctggct ctgcagtgaa ggtagttaca agattcaatt tgccgttttc
    19621 ggacacatca taagtataca cacgatctgt accaagatca catacgacta atcgttggtc
    19681 aggagtcaga tccgtatagt ggacatgcgc atgatcttgg tttttatgag gacctgtcgc
    19741 ttcttgatgg aatattgaat ctgctgcttt cagttcgcca tctcctaata tatggtagac
    19801 atttacttca cctttatgat agttcgcccc gtatagtaat tgacgtgctt catcaaccgc
    19861 tacataacac aaaggagcgc cagactctgt taccgcattc aaaaaatgaa aatccttatc
    19921 gtacgcaccg gcaccaccta atccatccac actagtaact gtgtaaagat tgccagcttt
    19981 actttttgct acataggtag ggctattttc ttcaacagct aatgaaaggt tggctaattc
    20041 ttctttttct gtatcaaggt cgatcgtata gattccttta ctttcgcgac gcgtataggt
    20101 tcctaaaatt atcttttcga tcatatgagc tgattcctcc taattgtaat gaaatagttt
    20161 tcgtttttat tctatcacat aattattcca cacggctaaa gaatgagaat gtgttatact
    20221 gtttttgaaa gcaattaaag gagagagtgc aatgattcag gcaaagatta cagaaattgg
    20281 gacagaagca ataaacaagg aagaacccat gctgattttg ttcgataaaa cagcaacagc
    20341 tgccttacga aaatattcgg tgattcaaga agtatcaaaa gacgaacctt tttctttgaa
    20401 agcaggcgga acaattgctt ttgatgatca ggtatatacg atcgattatg tcggtccgat
    20461 ggccaatggg aatttaaaga cagtagctca cgtatcattg atttttgatg cttgtccaga
    20521 agaaggacaa attgcaaatg gaatctatct tcatccgcat agactgcctg ctattggttt
    20581 gggaagccaa attttttata actaacagga ggaattatgg aaggaaaaaa taagaagaac
    20641 agaccggtat cgtggttttg gcgctggttt ttaaataatc aagtagtcac agccttactt
    20701 gttgtattgt taatattgct gatcgtcttt ttatttacaa aagtttcata tttgtttgca
    20761 ccaatctggc aatttttagc aatcgtaggt ctgcctatca ttttagcagg aatcttgtat
    20821 tacttgatga atccggtagt tgattacttt gaaaagcgaa aagtaccaag gctttacagt
    20881 atcattgggc tgtttgtttt ggttgttgca ctaatcgcat gggggagcgt agtgatcatc
    20941 cctaagattc aggaacaaac agtgagcttc atcagtaact tccctcaata tgtagatacg
    21001 attgataata aattaaccga gattttgcgg gacccgctgt tcaaccaatt tcgcgaacag
    21061 ttggaaacag caggtgataa gttcatgaat tcggcaggcg atatgatcca agatatatct
    21121 aagagtacag ttcagagtct tggaagtttt gtcggcgcgg tagcgactat tttagtggct
    21181 ctgctgacga tgccgtttat cttattttat ctattgaaag acggtaaaca attagcacct
    21241 tattttgtca aatttttacc gacacgcatg caaaaaccga cgttgaatgt gctgagtgaa
    21301 atgaatagtc aagtttcttc ttatataaga ggacaactaa ctgtggcttt tgctgtggca
    21361 atcatgttta tgattggttt tgcggtcatt ggtttagagt atgctgtcac gttggggatt
    21421 attgcgggtt ttttaaacct gatcccttac ttaggatcat ttttagcgat gattccggct
    21481 atctttttag ccattgtagc aggtccattc atgatcatca aagtactgat cgtttttgca
    21541 gtagagcaga caatagaagg acgcttgatc tctccattag tactagggaa tcagttagca
    21601 atccatccag taacgatttt attagttctg ctgacttctg ggaaattgtt cggaatcatc
    21661 ggtgtgatct tggggattcc tgtttatgca gcagctaaag tagtgattac tcatgttttt
    21721 gaatggtaca caacaatctc ttctctttat gaagaaaatc cgttgaaaaa gcagaaagaa
    21781 taacaaaaag agacagatgg gaaaatatcc aaggcacaaa tgctgctttg aagatttttt
    21841 cctctgtctc ttttgtatgc atttatctca atttttaaaa tgatttacct aaagcagcag
    21901 ctttatctaa tgcattttgc atcaactcat ctgcacggtc agggtcataa tcgattcctt
    21961 cgatgaatag ctgatctact tgatcgatgc ctacaaagtt taaaatacct tttacatatt
    22021 gagaggcaaa gtcttgtccg ttgtatacac cgccattaga ttggatgtgc agtgcttttt
    22081 tacctgtcac taatccttct ggcccgtttt ctgtatagcg gaacgttttt ccagctacat
    22141 tgacggtatc gaaccaagct tttaattttg ttgggatatt tagattccat aaggcgtttg
    22201 cgataacgat tttatcagca gcaaggaact gatcagtcaa ttcattgaaa cgagcaattt
    22261 tttgttgttg actttctgat aaggaagtga attctgcacc agcacgtaat gcttcccaac
    22321 ctgataataa ttcttcatcg atttccggaa tatcatcgcg ataaagttcc aaaacagtca
    22381 cttcatcatc tggatgattt tctttataac ttgttaaaaa agacgtcaat gccttaactg
    22441 tgcgcgattc ttctgcagtt aggggatgac ctttgactac taaaacagat gccatatgct
    22501 taccttctcc ttttattgct atgattgtta taactatgac taacaaaatc agtttactcg
    22561 aaaaagaaaa ggctatcaag tcatattgat aaggttgaag aataaataac taaaaaaaga
    22621 cagggtataa aagaggctgt gacacgattt tttgtcacag cctcttttga atctaacaag
    22681 caatattcaa gcaacactcg actgaagagc ttccatccca ccaatgatat aaacagcacg
    22741 aaaaccatta gcagtaagtt gatcggctaa tgcttctgat cgcctgcctg tatagcttag
    22801 aatataataa agtttctttg gatctagatt ttttaattgt cttggcaaga tagcagcggg
    22861 gatagaaagc gagtggctta agtggttttt tgtgtaatct aaggtatttc ttgtatctaa
    22921 aacgatgctg tcagcatcga gaggcaattt attgaagtct tggacactaa ttgattgggc
    22981 catttatctc atcctttgtt tagtatgtgg ctattttaat gggcaactct cattaattca
    23041 aatggaagga ttcctcttat cactttttta atgtatcgga tgataatgaa aaaaactatg
    23101 caaatgtctt gcaaaacagg taggaggtgt tataatgaca tagcgtcata tgacaatgtg
    23161 tcatcttgaa agaaaggaga atccatatgc cgacacagac atttttccat ttgcctaaag
    23221 aaaaacaaaa aagactaatc gaggctgcga gaatcgagtt ttctagagta cccttaaaag
    23281 aagcttcgat tgctaatatc gtcaaattag cggatatccc tagaggcagt ttctatcagt
    23341 attttgaaga taaagaggat ctttattttt attactttga gacaatgaga cgtgacagct
    23401 ctaaagatat gttacaacta atgaaagaat ccgatggtga tctattcaaa ggattcgaat
    23461 cctatttttc taaaatgatt tatgagattt tggaaggcga gaatgcttct ttttatcgta
    23521 acttgtttat gaacatggat taccgtgcct caagaaaagt cgctcctcat acgagtcgcc
    23581 aaaaaacgcc tcacgatcca cataaagaac acaaacaaga tgcaaaagag ttgcttgaat
    23641 tgatcgatcg gacgaagctg aaagtaaaaa atgatcatga attggaatta ctgatccaat
    23701 tagtgatgaa tactgtattt acaacaattg cgcattcgta tcgcttattt tcaacatctg
    23761 aagattactc agtagagcaa gcaatcagtg aattcaattt gaaactttct tggctgaaaa
    23821 acggagtata taaatagaaa gaagggaact agatgctaaa aatcgtaaaa cggatctcct
    23881 tgacttcagc aattgcagct gcagttttca tggtgatcca agtactggca gatttatatt
    23941 tgccgacact tacctccaat attattgata aaggggtagc gcaaggagat gtcgattata
    24001 tttggcatac aggttttgtg atgatcggct tttctttgat cagtatctta gctgctatcg
    24061 caaatacatt ttttgctact cgtgaatcac agaaactggg taagcaattg cgtacggatg
    24121 tctataaaaa atcagagagt ctgacaaaag atgcgtttga taaatatgga acggcctctt
    24181 tgatcacaag aacgaccaat gatgtgactc aaatccaaat ggttacacaa atgtttttac
    24241 gtatgatgat caatgcacca atcacgttga ttggcgcgag catacttgct tatcaaaaag
    24301 atcatcagtt gacaaaaatc ttcttagttg tcatcccagt aatgattatt ttgatcggcg
    24361 gaatcatgta ctttgctgtt ccattattca aaagtatgca aaagaaaact gaccgtttaa
    24421 acctggtatt tcgagaagga ttgacagggg tcagagtgat tcgagcattc gataagacac
    24481 gctttgaaga aaatcgtttt gatttagcga ataaagatta tacaaatacg gcaatcaaag
    24541 ttaatacgat cgtcgcattg atgatgccaa tgatgacatt gatcatgagc ggtacaaacg
    24601 tagcaatcac ttggtttggt ggacactaca tagcagatat gactttggaa gtcggtaatc
    24661 tgattgcatt tatgacttat gcgatgcaga tcttgatcag ctttatgatg ctttccgcta
    24721 tttttatcat ggtgcctcgt gcacaagctt cagctgaccg tattaatgaa gtgttggatg
    24781 aaaaaatcgg tatccatgac cctgaaaatc ctaaaacagt ttcttttgct ggaaaaaacg
    24841 caacattggc cttcaatcat gtcaattatc gatatcatgg agcagaaaaa cttgcattgg
    24901 aagatattga tttccaagcc aagtcgggcg agatcgtcgc aatcataggg gggactggtt
    24961 caggtaaaac gaccttagtc aatttgatcc ctcgattata tgacatcgaa tctggttcga
    25021 tccaaataaa tggaacggac attagtgaca tgactcaata taatttgcga gaattaatgg
    25081 gctttgttcc tcaaaaagca gttctgttct ctggaacgat ccgagacaat atgcagtatg
    25141 gtaagccgga tgcaacagat gaaatgattt ggaaagcatt agaaatcgca caagctaaag
    25201 attttgtttc agagatggaa gatggcttag atagccatgt ggaacaagga ggaggaaact
    25261 tctctggtgg acaaagacag cgtctggcaa ttgcacgggc attagtcaaa acagcagata
    25321 tctatgtgtt tgatgattcc ttctcagcgt tggatttcaa aacggatgcc aatcttcgcc
    25381 aagcattgaa aacgaatatg aaagaaagta tcaccgtttt agttgctcaa cgagtaagta
    25441 cagtaatgga tgcagatatg atccttgtgc tagatgaagg aaaactggtt ggcaaaggaa
    25501 cacatgaaga actattagca acaaacgaaa cttatcaaga aatcgttcat tcacaactaa
    25561 gagaggagga cttagcatga gtaaaacaaa cacacgtccc tctcatggac cagcaggcgg
    25621 tccaggacat ggtggacctg gcagaaatct cggtgtaaaa ggtgaaaagc ctaaaaattt
    25681 ctggggaacg gtacgccgat tgtttggcta tatgtctaaa cgtatggttg cgatcatcgc
    25741 tgtatttgta ctagcaatag cagccgtgat cttccaaatc caaacaccaa aggtcttggg
    25801 gaaagcaacc acagaaatat tcaaaggtgt aatggctggc gctcaacaga tgcaacaagg
    25861 ccaacaagta acaaaatttc cgatagactt tgataaaatc gcgcagatca ttgcgacagt
    25921 aatcgtgatg tatttgattt ctgctgtatt caacttttta cagcaattta ttatgacacg
    25981 tgtttcccaa cgaacagttt atgaattacg tcatgatttg gaagccaaaa tgaatcgtgt
    26041 accgatttcc tattatgata cacatacaaa tggggatatc atgtctcgag caatcaatga
    26101 tatggataac atcgcaagca cgctgcagca aaatttgact caatttgtaa ctagtgcagt
    26161 cacattgatt ggggtcatcg tgatgatgct ttcaatcagc tggcaattga cgttagtcgc
    26221 gttattgatg atccctttaa gcttgatcat cgtgatgatc gtagcgccga aatcacaagt
    26281 gtttttcgca gatcagcaaa aaagtttagg tttattgaat aaccaagtag aagaaacata
    26341 tggtggtcat accattgtta aaacattcaa tcatactgaa aaagaccagg aagtatttga
    26401 aaaagaaaat cagaatctgt atgccgctgg ttggaaagct caatttattt cggcaatcat
    26461 catgccgttg atgaatttcg ttaaaaatct aggctatgtg ttcgtcgctg tactaggcgg
    26521 agtaaaagtt gcaaatggga acatgacatt aggggatgtt caagcgttct tgcaatatac
    26581 aacacagttc tctcaaccaa tcactcaact tgcaagtttg atgaacacta tccaatcaac
    26641 agttgcttca gccgaacggg tatttgaagt gttggatgaa gaagagatgt ccaatgaaaa
    26701 atctggcctt ccagaagaaa aagatacacc atacaaagtt cgttttgaaa atgttcaatt
    26761 cggctacaca ccagataact tgctaatgac tgattttaac ttggatgtca aacctggtga
    26821 gatggtcgct attgtaggtc cgactggtgc agggaagacg acactgatca accttcttga
    26881 acggttctac gatgtttctg gaggtagtat tcgttacgat ggtgtagata cccgtgatct
    26941 ttcacgtgat gaattgcgcg cacagttttc aatggtactg caagatactt ggctgtttac
    27001 aggaagtatc tatgacaata tcaaatatgg gaacgacgat gcaacagacg aacaaatcat
    27061 tgaggctgct aaagctgctc acgtagacga ttttgttcgt aaattgccag aaggatataa
    27121 cactgtatta aacgaggatg caagcaatat ttcgcaaggg cagcgtcagc taatcacaat
    27181 tgcgcgggca ttcttagcaa atccagatgt cctgatcctt gatgaagcaa catctagtgt
    27241 agatacacga acagaaatac tgattcaaaa agcgatgaac cgcctattgg aaaaccggac
    27301 aagtttcgtt gttgctcacc gcttgtctac aatacgcgat gcagacaaca ttattgtgat
    27361 gaatcatggt tcgatcgtag aaacaggaaa ccacgataca ttgatggaaa aagacggatt
    27421 ctatgcagat ctttataaca gtcagttttc tgaagaagaa gcttcataga atagtacaaa
    27481 tttattattt tttttagggg ctgtgacaaa agtcgtgtca cagtcccttt ttccgaataa
    27541 acggtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgagaagct
    27601 atttttccgt tttgtatctc taatactcag gtcaaaccgc tgattttaca acctcttcct
    27661 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatagtcaac aaacctgaga
    27721 agctgttttt tgaaattgct ccttgtgact cggagttaaa cggcaggctc acaacctctt
    27781 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
    27841 aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt tcacaacctc
    27901 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
    27961 aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa
    28021 cctcttttta taagagtttt tactcattaa tttacaaaaa acaagcaaaa tgcgaatttt
    28081 ctcacatttt ttttaacaaa atcaaagtta ttgcttgaaa gaagaagaat ccatcgttaa
    28141 aataaagctt gacgtagagg ggagcaagaa atatgaaaga ttttcaattg ggggctgcca
    28201 cctatttaaa aagtgtagcc attcgggtga aagaccgaga taaaatgatt gatttttaca
    28261 agaacgttgt tggctttgat ttgaaaagag aagaaaacga attagccatt ttaggaacag
    28321 aagaagctgg aagtgaaatg ttgctgctgg aagaaagtcc tagagcgaat gatcacatgg
    28381 gggtgatcaa aaagctgaac cgtttttcat tgattatccc tagcacagaa gaaatggcag
    28441 atatcctttg tcgtatccgt aaaatgaatt atccaatcaa agaagcttta gaagatcatg
    28501 gacgtatggg gattttgctc gtagacccag aagagaatga attggaaatt taccatgaag
    28561 agaataagaa cgaccaaaga gaaattccag aaacaatgga tcaagaagca ttattaagaa
    28621 aatctgaaga aaaattcagt aaactatcaa caggttctta ttttgaaaaa atccatttaa
    28681 atgttactaa cttaacaaaa gaacgccaat ttcttaatca tgtacttggt ttgaaggtac
    28741 ataatgaaga agagggcatc catgtattga atgaaggaga tttccatgtt ggtttgacag
    28801 aagctagcgg aggcgcaatt gatttgccta cagatgaagt gattggactt gattttctga
    28861 aattcatgat cgatactgaa agcatggaag gactgattgc tcacttgcag caactatcag
    28921 ttgatttctt cttagacaaa aagcacacag tactcacaat ctatgatgct ataggaatcg
    28981 aatggtggtt cgtcagtgag ccaaaagaaa agaataaaac tgacattcga tgcgacgctt
    29041 aactttttct ttcttaacga ttgtcgcatg tctcgtcatt ttatgaacgg tcatactatc
    29101 gcttattcta gagatagaaa gagaaaatag tataccgggg gaaactgcgc ggtttttcgc
    29161 gcagtttttt tgtgtaaaaa aggttgattc ctcatagatt aagctttaag cttggactgt
    29221 aagaatctga tagaaaagca ggagaaaaag atgtatccac acacgaaaca gcttattttg
    29281 aaaaagatgg aagaaggatt ttatccaggt gtagcagtcg ccttcataaa agaaaatcaa
    29341 gtagaaacat ttacagccgg ctttgcccaa gtcgttcctg aaaagcagct gatgacagaa
    29401 gatctgttgt ttgatgcagc ctcactgacg aaagtcgttg caaccacttc attagtttta
    29461 cgtctattag aagatggaaa gatagaactc gatcaaccgc ttcataaata tcttcctgaa
    29521 tttgaaaatc agacaatcac tttgcgtcat ttactgacac atacttctga tatcaaaaca
    29581 tggataccaa acagagataa gctatctcag gaagaattga tcgccgctta tttgaagcta
    29641 gaaccaggtg agcaactagg aaaagaagta aaatatacag atgccggtac gattcttctt
    29701 gggttgatgc tagaggaaat ttaccaagaa actgcagtgg aaatctttca agaacaagta
    29761 ttggaaccat taggaatgat gaacagctta tttcttcctt ttccttccaa aaaaatcgtt
    29821 cccacagaac aactgccttc gggtgaggtt ttgcgtggac aaactcatga tccgaaagca
    29881 agagttttgg ctgaacatgc aggtaacgcg ggtctcttta ttgatttgaa agacagcatt
    29941 acttttgtcc aaatgtattt gaacaaggga aaaatccgta atggtcgttt tttagaagaa
    30001 gagacgattg attctttact gcaagatcaa acaccgaaaa aaaatggcta tcgttcttta
    30061 ggatgggact taaaaaagga tgtagaagac ggtcatgcga ttctttttca tactggttat
    30121 acaggtacgt ttttcttgat tgatataaaa acacaagaag cgttgatatt tttgtcaaat
    30181 cgcgtacatc ctgtcgatca tcgtgcggag tatctcatat gtcgagatga aattgttgaa
    30241 aactttttga aagagaaagc ggtattaccg aaataatgct ataatagcta tgaaaaatga
    30301 gaggagtttg aattatgcag gaacctatgt ttttaaaacc agtttttcaa gaaaaaattt
    30361 ggggaggaag tcgtttacgt tcagtctttg gttttgatat ccctaacgat aaaatcggag
    30421 aagattgggc gatcagtgcg catcctcatg gtgtcagtgt agtagaaaat ggcgaattca
    30481 aagggaaaag attagatgaa ctatggtcag aacacaagga attgtttggc catccttcag
    30541 aaccagtgtt tccattattg atcaagatct tagacgcaga agatgatcta tctgtacaag
    30601 tacatccaga tgatgcctat gggatgaaac atgaaggtga attaggaaaa actgaatgct
    30661 ggtatatcat cgatgctgaa cctggtgcag aaatcatcta tggtcatcat gcaaagacaa
    30721 gagaagaact agctgagatg atcaaagatg ggcgctggga tgaccttttg aagaaagtac
    30781 cagttaaaaa aggtgatttc ttttatgtac caagcggaac gatccatgct attggtaaag
    30841 gaatcatgat tttagaaaca cagcaaagtt ctgacacgac ttaccgcgtg tatgactacg
    30901 acagaaaaga tgcgcaagga caaacaagag agctgcatat ccaacaatcg atcgatgtaa
    30961 caactgtccc tgcgaaaaca ccagaattac aaatcaaaga agtccgaaaa gggaattcat
    31021 cgatcgtcac ttatttagaa actgagtttt tcaatgtata tgagtgggat attaaaggaa
    31081 tcacatcttt caaaaaacaa gccccttata cattgatgac ggtaatcgaa ggagcaggag
    31141 accttgtcgt tgacggaaaa acatatccat tagaaaaagg aacaagctgt attattccaa
    31201 atggcgtttc tgagtgggcg gtccaaggtg aactagcaat cattgcgtct gaaccaggca
    31261 aatagttccc agatagctga aagcagcaga cggtttttcg tttgctgctt tttctacata
    31321 aaatccgtcc atcaataaat agaagtctgg tgaaaaagat ggaatacaaa tggaaagcaa
    31381 acaacagcag tcgcgtgtct atgaaacaac tgagaaaagc tggacatcac ttaaatgaaa
    31441 tgatcatgcc aaatctaagt ctgtttatcg cttggggaat cttgtcatta gcaacaaaag
    31501 gtgtgtctgg cgatttgcac accacattag aagaggtatg ccattggatg atccagctgc
    31561 ttttacccat cttgatcagt tattcaggcg ggaaatcggt agggggacaa aaaggggcag
    31621 tcgccggtgc aattgcttct ttagggttga tcgctcgttc caatgctcca caaatcgtag
    31681 gagcgatgat tatcgggccg cttgccggca tcgtatatga acaatttgtt tctagattcc
    31741 atgtgaaatt caaggctgga tatgaaatgc ttgtcagcaa cctactagta ggactgactg
    31801 gtagcgttat ttgtataatt ggaatcgttt ttattgaacc agttttaaat agtgttcact
    31861 tgttgctcgc ctccgttgtg tcttggctag tcacaatgaa tctgttacca cttgtcagtc
    31921 tgatcttaga acctctaaaa gtactatttt tcaataatgc aatcaatcat ggtgtattga
    31981 cacctttagg aatcgaattc tcacaaacag tcgggtactc ttccttattt ttgatggagg
    32041 caaatcctgg acctggttta gggatcttac tttctatcct ttgttttgca gaaaaacagg
    32101 aaaaagtaaa tgcgagtgga gcattgatga tccatgcgat tggcgggatt cacgaaatct
    32161 attttccatt tgtcttgctg cgaccgtatc tatttttagc tgtaatggct ggtggggcaa
    32221 gcggcacgtt gatatttcaa tggttagata cgggattagc tgcacctgta tcgcctgggt
    32281 cactactcgt gatttttgcc aatgcgcctt atccacaact agctggggta agtataggga
    32341 tagcaatcag tacatttgtg accttcattt gtgcagcgat actattgaaa ttaccaaata
    32401 aagaaaaaga gaacaaaacg gtcaaagaaa ggggagaaat cttggaacca tctatcattc
    32461 attccgtcat ttttgcttgc gactcaggta tgggatcaag tgctatggga gccgccctgc
    32521 tgaaaaagaa attagcagca gaaaaaatcg atctttctgt cggatacagc tctatctatg
    32581 aattgaataa ccaagtaaat cagctggtta tcgtgtaaca tgaactagcg ggtcttgcaa
    32641 gagaaaaagt acctcaaagt caaatctatg tactggatca ctttttggaa acagatatct
    32701 tgctgtcacg aataaaagaa gcgatccaat ttccaaagcg ggaagaaaaa aaagagatag
    32761 cagatatcac agaacaaaaa acgtcgcctg ctaatattgt atttttgtat aaaaacaaca
    32821 gacgcggctc acaaactatg ggcatgacga ttttaagtca attagcaaaa aaagcgaaaa
    32881 aacagtttgt tatctcaaaa gaatcaatag aaaaaatcaa aatccaagaa gaaacgattt
    32941 atatcgcaca aagagaacta gctgtaactg aacagctaga agaaaagatc cctcggctag
    33001 ttctagttga acattgggta gctacgaatg aatacgaaaa actcgtgaag ggagaaatat
    33061 aagatgtatt tatcgcccag agaaaaacaa ttattagcag aatttctcag tagcccgtca
    33121 cctgtatcta tccaaaaaat gatgaattta ttgaaggtca gtaaacggac tatttaccgc
    33181 gaattagata atcttgaagt ctcattgaaa tcggtaaatg cttctttgaa aaaagcagct
    33241 agaggctcat ttgtcattga ggcatcagaa gaagtattag agcaattaaa agaaaataca
    33301 gatgggaaaa gtaatgactt atccactgct aaacgacaac acgcgatttt agccgagttg
    33361 ttactgacga cagagccgtt gagtcttgct catttcatgg aatcatatcg catcagtaat
    33421 accacttttt atgcagatat caaacagttg gaagaaagta ttcgccaact gcctttggaa
    33481 atcattcgga accagggata cgagatcgaa ggaccggaaa aataccgccg gcttctgaca
    33541 gcgaatgtat tagaattaga aatcaatgaa tatgaacttt ttcatagtat ttctttcgat
    33601 gcatcactga attatttttt tcgattcgtt gatccgcaac atctatctct tgcacgcaaa
    33661 gtaatcgggg aagaattgat ccaaaaaaag accaatctaa gtgatagaaa gcttgaacat
    33721 ttggttttga tgctgacatt gacgatggag cgtgtgacga aaaatcattt actgacaaat
    33781 gagacatata atagtctggc aaacaaagaa ttactacaga cagctaaacg attgttttca
    33841 cggatcgctt ctgagacaaa acaactttat ccagtaaatg agatcgtctt ttttgcagga
    33901 ttattaaatg acttcacgaa ctcatttgat cagaatttct ttgaagagac gttcgataca
    33961 cagcttgctt atttagtaaa acaactgatt caagaagtaa gtgaagagac gaatgtccat
    34021 ttttatgaag atgaaacttt gtacaaaatg ctgttgactc atttatctgg tgtgttttca
    34081 agagccgttt tacaagaaga gacactgacc aatccgattt tagaaaaaat catggttcaa
    34141 tacgaggaag ttgcgaatgc aatccgtaaa gcagtagagc ggatttttca agaaaaaaaa
    34201 ttatccgaag aagagatcgc ttatatggtc cttcattttg cgaattcatt ggaacgaagt
    34261 ccgaaagaaa gcaatatcag tattgcagga ttttcgccaa gtggattagc ttctactagc
    34321 atgttggaac tacgattgaa aaggtatttt cccttcatcc aacaaattca tttctatcgg
    34381 attgctgatt taaaaaaaat cgatttacaa gaagaatatg atgttgttat ttctacatct
    34441 attttaccag gatacggtgg aaaatatatt ttagtctccc cgctactgct agaagatgaa
    34501 gtcaagcagc taaaagaagc tttttcgcat attgagaaga aaaaaaggca aaaaattacg
    34561 tataacagca ataacgagtg gatgatagaa gatacttatg aggaaacaat gcatttcatg
    34621 gatcaaatca atcaattact cggtcgtttt tttatccaag aactggataa tcctgagacg
    34681 atttccgaaa cgattcgcag cttatttcct tattttcctg aaggactagt aacggatgaa
    34741 gaggtggttc atcgccggct gcttaagcga tatgaacaag cacctatcgg tattcctcat
    34801 acaaatatgg ggttattcca tacatccagc tcattgattt ctaaaccgat cttttgtatt
    34861 ttcaatttga aacatccgtt gaccattgaa gggatggata gacagccagt cgatttgtcc
    34921 cgtatgttag ttatgctggc gccttcgcca atccaagaaa aagacgggat tcttttagga
    34981 aagatcagtg gagcaatcat catgaatgac ttgaacaccg agatcttccg ctcaggaaat
    35041 aaagaaatcg tttatcagtt gttagcgaaa atcctgattg aagaaataaa gaaatgaagg
    35101 tgaaaagatg atacagctac cagcagaaaa aatattgctc gatcaatcct atcaaaattg
    35161 gaaagaagcg ttgaccgaga tcggccagtg gatgacccaa caagggggaa tcaaaccggc
    35221 atatatccaa tcaatgatcg aaagacaaga aaaagcgagt gtctatatcg ggaatttcgt
    35281 agccttgcca catgggactg acaaagatga agaagtagta gaagaaggaa tctatttagt
    35341 ccaagtgcct gatggtgtgg actttggaac aaaagaggaa ccaaaaatcg cgacactctt
    35401 atttgtagtg gctttaaaaa aagaacatca gttagcggtg ctccaagatt tagccttttt
    35461 ctgttcagac atcgaccaag taatggcgct cagtgatgcg gaaaccattg aagaagtcca
    35521 aatgattttt gaacatggac atatataacc aatgttcaca gtaaaaaaag ctgccttgca
    35581 tcaagtaata acttcaacca agataaaaat aagaggagct atttttgttg ggaggttctt
    35641 attactgcgg atgcaagctt ttgaaaacta tttattctag aaaaccgttt atttgaaata
    35701 ggggacggtg acaaggtttt tgtcaccgcc cctattattt ttgtgtttta atagctcttt
    35761 tttatataga tagtgcccat acctatgaat aataaaacga tactccatat accaagtgtc
    35821 aagataccat tcatgaaagt aacctgttct gaagctagtg gcgagaaaat atctcctcct
    35881 atgatcacat tttgaaaatc aacataagaa gttggcaagt aatgactgat tgattctatc
    35941 catccaatcg aagaaaacaa gcgatactct gctaagaaaa gacaaagaag tgtacctaaa
    36001 agattgacta gagaatgttc aaataaagca gatagaagaa aactgattgt acttaaaaag
    36061 agcacccaca acaacaggaa cagaaagctc tggaaaagat actcatacat cggcaggatt
    36121 cgtatctcct gaccaatgat cgttcctaca ggataacgga agtctcccaa accatttttg
    36181 attattacta gtatagagac gatcaaaaat ggcatcacga gacttagcat agctgcacca
    36241 taataaatgg taaaacggat gctatgtttc tttaccatac tatccggtaa tagattgact
    36301 agagagatta cctgctttct tttttcataa gagatgatgg aagaaagaaa caaggcggtt
    36361 atacataaaa tcaaaaaact agaaatcatt cctgaaaaaa tattttcgta gtaatttgct
    36421 aaagagagat gcgctggcaa gtcaaaaata gaatatcttt ggatctgatg ttctttcata
    36481 taacggagca actcgacacg tttttgttgt tcaatgattg gaattccttg tctttgacca
    36541 gaaatcagtc gatccagtat atctttttca taatgataag ttgcttcgac catttgctgg
    36601 acattccctg tgttttttgc atgtaagatt gcttcgatca gtttgttgct tttctctaaa
    36661 ctttccacaa attctttctc attctcatct tctttcaacg tttcaatcaa acgttgcatc
    36721 tcttcatagt tatgattcag ttcatcgaat tctgtataag caagtttatg atcaaaaaaa
    36781 agtgggaatg caaagagcac gaccaacaat agaggaaata aaaagtttac tttgtttttt
    36841 agtaagagat tgcgttcaaa tgtaagtaaa gacatagtta cacctcatat aatttacgat
    36901 aaatagctaa aatctcttcc atatttgagg attctgtgac gatttctcca ttttttagaa
    36961 aacatgcttt agaacaaatc gattggatat tcgcgatagc gtgggaagaa agaagaatcg
    37021 tcttcccctc tttctgccat tgtctgatga ggctattcaa gatatcaata cttcctgggt
    37081 ctaatccgtt caacggttca tcgaataaca tgacagatga atcactgatc aagtaagcag
    37141 caagcaagac atgttgtttc attcctagag acatttttcc aatcggcaaa gtaaggtact
    37201 cagtcatctt caatcgttgg aaaacagttt ggcattctac tttgctctgc caatagtatt
    37261 tgatcaaagt caaataatca acagccgata gatagtcgga taactgattc gttgattcta
    37321 gaaaaaagaa agaccggcga taatctgtat aagcagtagg agagatgttg tgaagggaca
    37381 attgatcata agttgttggc agcagttggg caatgccatt cagcaaagtt gttttgccaa
    37441 atccgttcgg ggcaatcaaa gcagctactt ctcctgattc tactaaaaga ttcaattgtc
    37501 gaatcacacg ttttttacca cgtttcatgt tcatgttttt gatcagtaat gtcatagata
    37561 ggcctccaat ataaaattta ataacataat agaatggaaa acatctaaat taaatgctaa
    37621 caaaagttat tgtttgcata ttataaaaaa attaaaataa cagttttatt taaaattgaa
    37681 gttttctgat accgaaatct ttttattttg ctgacatact caaacctttg gcacaaagtt
    37741 tcgtgccaaa gtacgactat ttctcttttt agtaagcgga tacaattaga gtatcaaaag
    37801 gagagaggga gagacaaaat ggaacggacg attcccaaaa acaacatttc gctaaaagcg
    37861 agggtacaaa aattaggcag tactttatca agcatggtca tgccaaatat tggagcactg
    37921 attgcctggg gtgtattaac ggctctattt atcccagatg gttatttacc aaatgaggct
    37981 ttcgctacca tggtaagccc aatgctaact tatctgattc ctttattgat cggttatact
    38041 ggcggaaaag taattgctgg tgaccgcgga tcagttgttg gggcaattgc aacgatgggt
    38101 gtgattgtcg gaacagatat tccaatgatg ctaggcgcga tgatcatggg acctcttggt
    38161 ggatatgcca tcaagaaatt tgatcagcta ttccaaaaac ggatcaaatc cggttttgaa
    38221 atgctagtca ataatttttc agcaggtctt atcggttttg gattagcgtt attaggtttt
    38281 tctgcgattg gtccagttgt cgatgcattg actcaagcaa tggcaaaagg tgtagagatc
    38341 attttatctg cccatttgat tccattgaca agtattttta ttgaaccagc aaaaatctta
    38401 tttttgaata atgcaatcaa tcatgggatt ttgacaccat tgggaactga acaagtagca
    38461 gaggctggaa aatctattct attcttgctt gaagcaaatc ctggtccagg acttggcgta
    38521 ttacttgctt ttactttgtt tggcaaagga gcagcaaaat cttcagcacc aggagccatg
    38581 atcattcact ttttaggcgg gatccatgaa atctattttc cttacgttat gatgaaaccg
    38641 ttattattct tatcagttat aggcggagga atgtcaggaa gtttcatttt ccaattgatg
    38701 gatgctggac taagagcgcc agcttctcca gggtcgatca ttgcgattct agcgatgaca
    38761 ccgttcaatt cttaccttcc tgttattttg ggcgtgattg ttgccgcagc tgtttcgttt
    38821 ggtatttctg cagtaatttt gaaagcggat gcgaaagata caaatgaaga cttcgaaaaa
    38881 tctgttcaag agacaaaggc agctaagcaa gcttccaaag gtgcccaagg tattcctcaa
    38941 ggagctacat tctctggcgt acaaaaaatc atttttgctt gtgatgcagg aatgggatca
    39001 agtgcgatgg gcgcttcgat cttgagaaaa aaagtacagg aagcagactt gcctcaaacg
    39061 gtgaccaatc aagcaatcag ccaactgaca gatgactcga atacattgat cgttactcag
    39121 gtcgaactac aagaaagagc aaaacaaaaa gcaccgagcg cgcgttttgt ctcagtagaa
    39181 aactttttga attcacctcg atatgatgaa atcattcaag aattaagcag caagcagaaa
    39241 acatcagttt ctgaaattga gaatgagcca gcagaagagt ttctttcagc aaacatcaaa
    39301 caaatcaaag agattctact tgtccacgat gatcgttccg gatcagcaac aatggggatg
    39361 accgtgctca aagatatatt agacaagaac catttaacta tcccgttaag aaaagtcaat
    39421 atccaagatt taaaagatga acctgattca ctgatcgtag ccaaagaaag tttagcggaa
    39481 aaagtacaga tggctgcacc tcaatccatt catttatcag tcaacagcat ggtcaatccg
    39541 caaaaatacg aatcgattgc acaatatttg aaacagacag cttaagggag gaaaacagca
    39601 tgcaaagttt gccattcgac atgatcgagc tcaatcgatc ttttaaaacc aaagaagaag
    39661 cgatccgcta ttgcggtcag atgcttgtag aagcagggtg cgttgatgag gaatatatcg
    39721 atgccatggt agatagagac aaaatgttat ctgtttatat ggggaatttt atcgcgattc
    39781 ctcatggaac agatgaagca aaaaaatatg tgaaaaaatc cggcatctgt gtcgttcaag
    39841 taccagatgg tgtagatttt ggtaccgaag aagaagaaaa gattgcgact gttttgttcg
    39901 gtattgcagg agtcggagat gagcatttgc aacttgtaca gcaaatcgca ctttattgca
    39961 gtgatatgga caatgttgtc cagctagcag acgcgctcag caaagaagaa gtaacggaaa
    40021 atctagcaat tgcttaagga gagagaaaaa tgagagcagt acattttggt gcaggaaata
    40081 tcggtcgagg atttattgga gaggttttgg cagctaatgg atttgagatt actttcgtag
    40141 atgtgaatca cacgatcatt gatgctttaa atgaaagaaa agaatacgaa atcgaattag
    40201 cagatgaatc aaaagaaaaa atcactgtaa aaaatgtcaa aggaatcaac aatcaaacaa
    40261 atccagaaaa agtaatccaa gaaatcgctg aagcagattt agttactacc gccattggcc
    40321 cgaatatctt accttttatt gctgagttga ttgcaaaagg tatccaagaa agagaaaagg
    40381 aaggaagcac aacatcttta gatatcattg cctgtgaaaa tatgattggc ggttcagcat
    40441 ttctagaaaa agaagtgtat aaatatcttc cggaaacatc atttactgac aaatatatcg
    40501 gctttccaaa tgcagcagtc gatcgaatcg tcccattaca acaccatgaa gatcctttat
    40561 tcgtccaagt ggaacctttc aaagaatggg tcattgacga tagccaaaga aaaaataaaa
    40621 aaatccaatt aaaaggggtt ttatatgtag atgatctaga accatatatc gaacgcaaac
    40681 tatttagtgt caatactggt catgcgactg tcgcttatac aggtgccttg ttaggttacc
    40741 agaccattga tgaagcaatg caagatgcgt tagttgtggc acaattaaaa tcagttttgc
    40801 aggaaacagg aagtttgctg attgccaaat gggggtttga tgcagagcag catcatgcgt
    40861 atattgaaaa aatcattcat cgcttccaaa acaaaaatat ctctgatgcg atcacacgtg
    40921 tcgctcggac accattacgt aagttaggtt atcaagaaag atttacacgt ccagtgagag
    40981 aactacaaga acataacttg acttgcccac atttgacggc aacaatgggg attattttta
    41041 attactatga tcctgaagat gaacaaagcc gtcaattgca tgaaatgaag atccatgaaa
    41101 acttggaaca attgattcaa gaagtcacag gaatcaatga tccgaaaaca atcgggaata
    41161 tcaagcaaaa cgtgaaccgt tatgccaagc aagtcgctta aaataggata cgaaaaaggg
    41221 gaatgtgacg aaatttttgt cacattcccc tttttcgaat aactaggctg tttgtccttc
    41281 aagatagggc agatccaatt ctttaccgta acgttgttct aaagctaggc ggatcaaacg
    41341 agctgccagg ttctcatttc ttgcaaggat aggtccatgg aaataagaac cgaagacatt
    41401 tcggtaaatg acaccttcag attggtcttc accattattt ccttgacctt gtaccacttt
    41461 ccccaaaggt cgttcacctt cacctaagaa agtccgacca ttgtggtttt caaatccata
    41521 ataagtttca ttgaattctt cgttatggat aacgatatca ccgataaacc ggttattttc
    41581 ctgacttaac gtgtagtgat ccagtgcact gattccttgg atcttttctc cattagctcc
    41641 catgtagtag tgacctagca attggtagcc accacaaatg gctaacatga cgccatcatt
    41701 ttcgatatag ttgatcaaag aatctttctt tttctggatg tcttttgaaa tgattaattg
    41761 ctcaaagtct tgaccaccgc caaagaaaac caagtcatat ttatcagcat caaaaggttc
    41821 atgtatacta acgatctccg tatggcaaga aacccccatt ttttctgcta tatatttcaa
    41881 catcaaaagg tttccgttat caccatatgt gttcatcaaa tttccataaa ggtgggcgat
    41941 attgagttca taattagcca cggttcattc ctcctttgat aaatccttga gcagctagtt
    42001 ctttacgaag ctgcaataca gcagtgtaag tagctaaaat atagacatgc tcagttggca
    42061 attctttgat tttttcgatt acttctggta attcttttgt ttctgtcaat ttattttctg
    42121 gaatcccggc aacttttaga cgtaaagtca tatctgtatg acggtctcct ccagaaataa
    42181 cagctgggat atccatatcg gcaaagtttt caaaattacc atcccaaatc cagctgacat
    42241 cgattccgtc tgcgtaattg gcatttaata aagccactaa agaaaatgga taaggagcta
    42301 gaccaatcat atcgattact tgattgatac ctacaggatt tttgacaagt actaatgtac
    42361 attttttccc attgatattg atgacttctt gtcggccgaa tactttctcg tcatatccta
    42421 aaccttgttt gattttatca ggatcgacat tgaagtactc tgccactgca gtagctgcta
    42481 aagcattgta gacattatac ataccgccaa cttcgattcc gtattcatga ccatcgatca
    42541 cgaaatctgc tgacacattg tccatccgta ccatatcagt taatcgataa tccaattcag
    42601 gacgtttgaa gtcacaattt ggacagtaat atttccctaa gtttgcataa gtgatcattt
    42661 tataatgcaa gatgtgatga caatttggac ataagacacc gtctgtattg taatgagcca
    42721 tttgttcacg atcttcttca tggtcaaagc cataatattt tctaggattg actgtttcga
    42781 tagaattgaa gattggtgaa tcaccgttac tgataatcgt tgcatttggt gactttgcgg
    42841 caccgtctac aatcatttta taggtcgtat agatttctcc ataccgatcc atttgatcac
    42901 ggaaaatatt tgtaaataag aacagttctg gtttcatata ttctgtgact ttgctcaaac
    42961 ttgcttcatc tatttctagg acagcaaact ttttctgtcc tttttttggt tttgcctgca
    43021 agaaagtcga tacaattccc tgtaccatat tcgcaccagt agggttggtt agaacttcat
    43081 cgaattgctg gcgcaaaata ttgactgtta aagcggtagt caatgtttta ccgtttgttc
    43141 cggtgacaac gacgacttgg tagtcttttg ccaaactatc tagtatatgt ggatcgactt
    43201 tcagcgctag tttacctggt aaacttgatc cgcctttaaa aaaagttttt aatgcccatt
    43261 gcgacgtttt tccaactgca atcgcaaaat gactgcgtat tcccattatg taacctccaa
    43321 aaacgatttg ctgacgaata aattcaacta atcataccat atgttgaaaa aaaggttaaa
    43381 ttgcttttct tattcttaag aaaaggttac ggctgaaagt gcatgaaatc ggacaaaatc
    43441 gcatgattta acaaaaaaac agcttgaaac aaaatgattg tttcaagctg ctgtgatttt
    43501 attattccct cttacctata ttcccatttt aaccgataat aattttctcg cttggatatt
    43561 catagcctag atctcgtgtt tcttttggca cgaataacat caatgtatat agcattccga
    43621 tccgcccaat aaacatcagt gtagcaatcg tgattttgcc cacaacagac aagtctccgg
    43681 taatccctaa agataaacca gttgttccaa acgcagaagt tacttcgatg atgatcgaga
    43741 tcagcgtttg ttcttctgta gcagataaaa aaacgataca aaagaaacac ataccaagag
    43801 aaagcataaa gacaacgact gattttcgca catcatcttg atcgattctc cgtccaaaaa
    43861 tattgatgtt atcttcactt ttgaggaagg aatatagata caaaccgatg attgctacag
    43921 tagttgttcg aatcccgccg cctacagaac taggactgca tccgataaac atcagtaaag
    43981 aaaaaatgat cagtgttgtg atttgaaaat cacccaaatc gtgaatctgc agaccagcat
    44041 tacgagtcgt aatcgaataa aacatcgaat tgatccattt gacactcata ttggaatctt
    44101 ggaataaatg gtccttttcc aatagataaa tcaagacagt tcccgaaaca aacaagataa
    44161 caaaagctaa cacagctaat ttggtaaaca aggaaaaacg aaacggaagc ttcgcattgg
    44221 aacgtttgta taatagccat tcccggcact ccatcaatac aggaaaacca attcctccga
    44281 caaaaatcaa aaacatgatc aggatcaaga aaaaatagtc atgtgcaaag ggcttgatgg
    44341 aatccccagt gacgtcaaac ccagagttcg tgacagccga tatcgcctga taaaaaccgt
    44401 aaaaaacggc atcacgccaa cgatcaaaat agcctctgta ataaaaataa atagaaaaga
    44461 atgtcccaaa aagtaattga aaccagatta aaatagcaaa agtgatccga atcagtcgga
    44521 cgataccaga caaacgcggt tgattcatat ccgtcatgat cagctgtcgc tgtttcagtg
    44581 tgatccgtcg tttagaaaaa atgacaaaag cagtagaaat catcatgatc cccaaaccac
    44641 caacttgaaa taatatttcc agcagtataa taccgttatc attgaacaca gaattgatat
    44701 caaaagtcga tagtccagtc acactcactg tactgattgc catgaacagc atgtccacaa
    44761 aagaaacatg gctatctggt tcgcgaaaaa tcggcatata aaatagaacg agtgataaga
    44821 ccgtcatcaa tatataataa aaaacgatga tttggatcga tgagaaatga ttggcagcaa
    44881 acttctgtcc tcgccgctgc agccttcgaa agaataaatc aacatgcaca gtactatcct
    44941 cctgacaata gtcgtttcta ccagtataca aggggaatta ggaatactca aagaaattat
    45001 caaacagata acaatacagc tttcgattaa aatagaaatg gcttataata aggaaaatcc
    45061 tagaaaaaag aaggaggagg aataacaaat tgattaatgc tcaacaaatc gtagaaaaaa
    45121 tgaaaaatca aaaaataaat tatgatacag ttctgaaaaa aatgatcatg caatgggaga
    45181 aagaagaagt aaagcctaag atattaatac atacatgctg tgctccttgc agtacgcaca
    45241 cattggaatt catgtctcaa tatgcagaga tcacgttatt tttttcaaat tcgaatattc
    45301 atcctaaaag tgagtatttg cgaagattac atgaacaaaa acgatttgta gaccaattta
    45361 atgagaaaac tgaccatcat gttgcgctaa tcgttgatga ataccgtcca aatgaattca
    45421 ccaaaatggt tttaacaaac catcttgaaa acgaaaaaga aggtggtgct cgctgcagtg
    45481 catgcttcaa tatgcgatta gatcttgttg cgcaaaaagc gcaagaacta ggatacgatt
    45541 actttggaag tgccctgacc atctcgccaa agaaaaatgc agctttgatc aatcagatcg
    45601 gaatggatat ccaaaagatt tacgggacac aatacttgcc aagtgatttc aagaaaaaca
    45661 aaggctatga acgctctttg caaatgtgca aagagtatga tatttatcgc caatgttatt
    45721 gtggttgcgt gttcgctgcc aagcagcaag gatgcgatct gaaagaaatc aatcgggaag
    45781 caaaagctta cttaaaaaca tcaaccgtag tttttgagga aaattaaaga aatctttcgg
    45841 aaaaacgtaa gaaaaaaata aagaatttat ggtagaaata aggtagactg tataatttgt
    45901 ccgtagtaaa gtaaggaaac aagaataaat ttcaatgaag gaagagataa gatgcgaaaa
    45961 gtagcaccta ttttgaagcc aatcgctgta ttaacaatct cgacgattgt cgcgactact
    46021 acaggtattg tgaatagttc cagctaccgt gcaccaagtt caagttctag tactgtagaa
    46081 gaaacaacac caactacttc agtctcgaca tctgaaccaa caacaacgga aagtttgaat
    46141 gaaaccacta cgacaagtac atgggacgat acaacaacta gttcgtcgta tgatgagaca
    46201 tatacaactg acagctcaag cgaaacaagt aatacggtac cagatggcac tccatcggat
    46261 tcaaccaatc caagtacaag tccggataca agtgctagct ccgaaacgat accaagcagt
    46321 acgactgtag atgataacac gcaaaatgat gatgcagcac gtacagcaca gcaaaaccaa
    46381 gattcaggaa atacccaaac gtataaccag caagcaggtg attctcaatg acatttttac
    46441 tagaccttat caacggggtg cataaattcc ttggctattt agacatcagt cctaaatatc
    46501 ttaaccgtgg atatacgatt ttaagtgtga ttcccacgct ttatttattg agaattgttt
    46561 atggactttg gcaaaaccaa aactatcttc aatttttctt atatggactt gcatttctag
    46621 tgcttgttta ttttactgtt ttaaatgtat tttattactt tttagataaa aacacgaaag
    46681 ccgatgtcac tcagcttttt gtgaagtatt tacctgatga agcgttcaac atccagacag
    46741 aagaatcgat agataatgga aattcgatcg ataaagtgaa tacacaagag gtcatggttt
    46801 cttatgacga agattatcaa ttgaaattag ccgagaatat gcggtatttg attggaaatg
    46861 gtgagatcaa aacaaatgaa ctcggtcgca tggatggatt tctggtcgat agaaatacat
    46921 tgtatcctta ttactatgtc aagaaaacag gagagaaaga gtatgttcta aatattggac
    46981 gaagctactc ggacatggaa aaagtaggga ccattcgact tgactcaaca gaagacacat
    47041 tggaacctgt cggattgttc atcgtcggcg gtgattttgt caaagacggg ttacgctatc
    47101 atgagcctta tcgtctgaaa ttgattgcaa aaaaggcaca gtcgcaggaa gaaccagtgg
    47161 tttatagtcg tagccaaaga agaagacatt cataatgtga tgaaacgccg tgtatcagtg
    47221 gttgcgataa aaacgtttat tcggaataag ggggatgtga cacgtctttt gtcacgttcc
    47281 ttttttatat aacgagaaag ataagacgaa cacatcactt attctttcct ttcatttata
    47341 aacattgtag aataaagaaa gtacaacgaa gtattgtgaa aaaacgtaat aaataacgag
    47401 gaaaatacaa gtaggaggta agctgtgaaa gaatttttga aaaattgggg gattttgatc
    47461 cttgtattag cggctatttt attagctaga gtgtacgtgt ttacaccagt tactgtaaac
    47521 gggcattcga tggaccctac attaagtgat ggacaacgat tgatttcatc taagatctca
    47581 aactatgaac gaatggatat tattactaca aaggaaccgg gggatgaaga acggatgatt
    47641 gttaaacgga tcatcggtat gcctggagat acagtcaaga tggaaaatga ccagttgacc
    47701 attaatggca aaaagtatga tgaaccttat ctggatgagt tcaaaaaaga attttcagaa
    47761 gataagttac aaggagaata tgcttatagc tcaggattcc aagcacaagc agaaagttca
    47821 tccaccttta cgagtgactt tgaatatact gtgccaaaag gaaagtatct tgttctaggc
    47881 gataaccgct tgatttcaaa agacagccga atgtttgggt tagtcgacaa agatatgatc
    47941 caaggaaaag ttgttttccg ttattggcct ctttcagaaa ttaaaatcat gtgatgaaaa
    48001 aataaaaaga agctgtgaca tgttttttgt cacagcttct ttttccgaaa attccttctt
    48061 atttcttgga gttgctcact ttttttgcaa tctcatttta agctttttgg ctgatggata
    48121 agaaaagcga agcgtttttg ttcataaata gcttttcccc acgatggaag gtatgactag
    48181 tagcagagac aaagcccgtt ttttccaatc gattattcaa ttcttcttgg ctaaagccat
    48241 tatggacttt cggatgacta atttgttcat tcttgtcaaa gtctacgatg atcaattgac
    48301 cgccaggagc taaaatttca taaagttttg taagaatatt ctccgtatca ggaatgtgta
    48361 aaagtacaag tgatagaagg atgagattag cttggacagc aggaaattcg acagaagcat
    48421 cagcgtggat cgttcggaca tttttcagat ctgctgcttg gatcttttct tctgccattt
    48481 tcagcatagt ttctgaagca tcggcaatga tcagttcttt gaatctttcc gcaagcggta
    48541 aactaacaag cccagttccg cctccgtaat ccagcaggac tttttggtgg ctgtcgcgcg
    48601 gtagataagt agttagttct tcgcgaataa tcttggctaa tgcttgacgg tcaggggaat
    48661 cataatggtt agcgatggaa tcaaaaatat tttttgtcat caaatagctc cttacgtaaa
    48721 actttgaaat tttaacttat tatagcacgt gaaaagaaag aaagcatgct atactcaatt
    48781 caaaaaggga gaatctagaa agaatatgcc gatatcgttt gtaaaagata gagaggaaaa
    48841 aggaaagtgt gtccgagaaa tattactaga cttgcctgag tggtttgggc ttcctgaatc
    48901 aacggaaaaa tatatcgagg aatcaagtaa actaccttta tggtgtgaaa aaagaaaaga
    48961 agaatattta ggctttatca ctctttcaca gacaagtgaa gatactgccg aaatctattc
    49021 tattgtatgg gaataaaaaa agactatcat caccaaggat taggaacaaa actttttaaa
    49081 gaagcagaag gttacgcagc aaaacattac aaatatttgc aagtgaagac tgtagacgag
    49141 gggcactact ctatatatga tcaaactatt tgtttctacg aatcattagg attttcaagg
    49201 ttggaagttt ttccaaattt atgggatgaa tggaacccat gtttagttct tgtcaaaaaa
    49261 ttggaacaaa agtgagcaat agatttaata ctaatctgtg cttgatgaaa attctcactg
    49321 taaaaaatgc tgaaaatcaa gtataatacc ataggaacaa actggaaggt gaaaaagagc
    49381 atggcacaac tattttttaa gtatggcgca atgaatagcg gcaagacgat cgagatccta
    49441 aaagtcgcac ataattatga agaacaaaat aagccagttg tcttaatgac aagcggtatt
    49501 gatacaagag atggtttagg cgttgtttca agccgaatcg gactgaaacg taatgcgatg
    49561 ccgatatttg aagaaacaga cgtatttgac gtaattcaga aaatggaaag caaaccctat
    49621 tgtgtgctga tcgatgaagc gcaattctta aaaaagaaac atgtcattgc ttttacgaga
    49681 attgtggacg aattggatat tccggttatg gcatttggtt tgaaaaatga ttttcgaaac
    49741 gagttatttg aaggatcaaa atatttattg ctttatgcag acaaaatcga agaaatgaaa
    49801 acaatctgtt ggttctgcca caaaaaagca atcatgaatt tacattatat tgatggaaac
    49861 cctgtttatg aaggcgatca agtgcaaatt ggcggaaatg aagcttatta ccccgtttgt
    49921 cgaaaacatt actttcaccc atcaatgatt acagaaggag aataagcatg tacgatcaat
    49981 tacaagcaat tgaagaccgt tatgaagagc ttggagaact tttaagtgac ccagaagtga
    50041 tttctgatac aaagcgtttt atgcagcttt caaaagaaga agcaaacacg cgcgaaacag
    50101 tcgaagttta tcgtcgttat aaagaagtag tacaaggaat caccgataca gaagaactgc
    50161 ttggtgaaaa actggatgat gaaatggctg aactagcaaa agaagaatta tctgaattaa
    50221 aaaaagaaaa agaagtctta gaagaaaaaa tcaagatctt gcttttacca aaagatccaa
    50281 atgacgataa aaatatcatc atggaaatcc gtggagcagc aggcggagac gaagcagcat
    50341 tattcgctgg cgatcttttc aatatgtacc aaaagtatgc tgaagcacaa ggctggaaaa
    50401 cagaagtgat ggaagctaac gttaccggaa tcggtggata taaagaagtg atcatgatga
    50461 tcaccggaga caacgtatat tcgaaattaa aatatgaaag tggtgcgcac cgcgtccagc
    50521 gtgttccttc aactgaatcg caagggcgta tccatacttc caccgcaaca gttgtcgtga
    50581 tgccagaagc tgaagaagta gagatcgata ttgaagacaa agatatccgt acagatatct
    50641 atcatgcttc aggtgctggc ggacagcacg tcaataaaac agcatctgct gtacgtttga
    50701 ctcacttacc aacagggatc gttgttgcta tgcaagatga acgttctcaa atcaagaacc
    50761 gtgaaaaagc aatgaaaatc ttgcgtgctc gtgtgtacga taagatccag caagaagcac
    50821 aaagcgagta tgatgccaat cgaaaatcag cagtcggaac tggggatcgt tcagaacgga
    50881 tccggacgta taatttcccg caaaatcgtg tgactgatca tcgtatcgga ttaactatcc
    50941 aaaaattaga tcagatttta gcagggaaat tagatgagat cgttgatgca ttgatcatgt
    51001 atgatcaaac atcaaaactg gaagctatgc aaaatgggta aaatgaccta tcgagaagtc
    51061 ctgtcacggg cttcttcttt tttagaggat catgggaaag aagcatatag tatccaattt
    51121 ttatttttgg aacgaaaaca atggaaaaaa cttgattggc tccttcatat gaacgaagag
    51181 atttcagaag aagaacagcg gttgatcgaa accgatctgc gacttctgct tgcagatcat
    51241 ccacctcagt atttgctagg atatgcagat ttttatgatc atcggttaaa ggtaactgaa
    51301 gcaacactga tccctcgacc agaaacagag gagttagtcg aatggtgttt agacgagacg
    51361 cctggtgtac cgttagaagt gatagatatt gggacaggga ctggagcaat tgctattagt
    51421 ttgaaagcag caagaaaaaa ttggcatata tctgctgtgg atctttcaga agaagcatta
    51481 gaagtagcga aagaaaatgc tcagaaagaa ggaacgaaaa tcagttttta tcatggagat
    51541 acattaggac cagtaatgga tcagcaattt gatgtgatta tttctaatcc gccgtacatc
    51601 agtcggaatg aatgggaact gatggatgaa agtgtccggt cattcgaacc taaaatggcc
    51661 ctttttgctg agaacgatgg attagctatt tatgaaaaaa tagcagttga agcaccaagc
    51721 gtactaaaat caaatggaaa gattttttta gaaatcggat ttcgccaagg tgaagcagta
    51781 aaaaaaatat ttcagcaagc atttccagat aaaaaagtgg cgataaaaaa ggatcttttt
    51841 ggcaatgaac gaatgatcca agtttatggt tgaagttgtg gatgaaaaat tttttatttg
    51901 tttttttatt ataaaaaaac acttatttga caaggatgta aacgactttt tggtttatca
    51961 acaagtttat ccactattga caaatagatt atccacaaac agtggaaaac ttgaggataa
    52021 actcagacaa aataagaatg tatgaaaaag gggttgtgga taaactgtgg aaacgaaacg
    52081 atatgatgaa acagaattgc aagaggcagc cgaagcactg aaagaaggag aacttgttgc
    52141 ttttcctacc gaaacagtat acggattggg cgccaacgct cttttaccaa acgctgtcaa
    52201 gaaagtgttt tctgtaaaag ggagaccgca agataatcca ttaatcgtcc atgtcgcctc
    52261 ttttgaacaa gtaaaagagt atgtggacaa ctttcatccg gcagcagaga agattgtgaa
    52321 gaacttctgg cctggaccat taactttgat atttaagatc aaaaaagaca ctttgccctc
    52381 agttgtgacg ggaggattat ccacagctgc atttcgtata ccggataaca aaaaaacact
    52441 agaagtaatc gaactttccg gtgttccgct tgtcggtcca agtgcaaata cgtcgggaaa
    52501 accaagtcca acgactgcag atcatgtata tcatgatcta caaggaaaaa ttaccgggat
    52561 tattgatgat ggagctacgc gaattggtgt agaatccact gttcttgatt taagtgatcc
    52621 aacagccatg cctatgattt tacgaccagg tgcagtaacg aaagaacaaa tcgaagcagt
    52681 catcgaatct cctgttgcta ttgatcagca tcttgtaaaa gaaaatgaaa caccaaaagc
    52741 ccctgggatg aaatacaaac actactcacc cgatacacgt gttctgatgg tcagagatgg
    52801 cgattggtca actgcagttc aatgggcaaa aaataagaag atccgtgcag gtgtcatagc
    52861 aagccccgaa attgcggatc aagtgcggac agatactgca gctgtttata tgtataacga
    52921 caactcagta gaagcggcgg ctaaaggatt gtttgcaggt ctaagaggat tagatgaacc
    52981 aacacttgga ttagatttga tttttgtaca agtgtatcca gaaactggat tggggaatgc
    53041 ttacatgaat cgcttgaaaa aagcagcagg acaaaattat tttgaaaaat gatcaattgg
    53101 ttattgtcgc attcttttag tatgatacaa tgctatcaag taaatcttct atcagtgaac
    53161 gaaaaactaa gcgtaactaa tcgtaataga agagatacga atatttggca atttgttttt
    53221 gatgggaaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg tagattacaa
    53281 aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac aagaacataa
    53341 tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg cacaaggaag
    53401 tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg gtgggtgtga
    53461 atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat tcggagcgaa
    53521 atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt atctcgcttt
    53581 ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac atctaacaca
    53641 tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg gtgttgatcc
    53701 gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac atcaaccaaa
    53761 attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga aattcagaga
    53821 aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg caggattagt
    53881 agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga ctaccactca
    53941 taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag cattagcaaa
    54001 aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc atgtgattgc
    54061 gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat atagtgaaca
    54121 aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg gaaccagagt
    54181 catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt taggtataaa
    54241 tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata aaaactcgat
    54301 tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca cgccagcaat
    54361 cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag ttgtcaaagc
    54421 attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg tacgcgaatt
    54481 aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt aaacagaaaa
    54541 gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga caatatcggt
    54601 gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca gggaggaaaa
    54661 gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga tgcaaatgcg
    54721 cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact agaagaagta
    54781 caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt ggatactggc
    54841 gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga taaattacca
    54901 gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac ccatgataaa
    54961 ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga atatagtcga
    55021 tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt gcaacagctg
    55081 aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc aggagattca
    55141 gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa gggcctccca
    55201 atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac agattcctat
    55261 catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt ttgggaccaa
    55321 tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag agcaaatata
    55381 aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga tgtattaaga
    55441 gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt tacacaagta
    55501 cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca gacaaaggca
    55561 acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata agctttgctt
    55621 gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc gagtttgact
    55681 agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa ggagagctta
    55741 aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac taactattat
    55801 tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa tcgctatgtt
    55861 gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg aaacaccgat
    55921 cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc caatcttgcg
    55981 cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta aagtgggaca
    56041 tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg tcaaacttcc
    56101 agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa caggcggatc
    56161 agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta aatttgtttg
    56221 tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg atattgacat
    56281 ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc ctggactagg
    56341 ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg gtgtgacaca
    56401 acttttgtca catccttttt tggtcatttt taacgtttga tgcagaacag ctttttcaca
    56461 gctttcgttt gcatataaaa aaaccatccc aaaaatgcag agatggtcaa attcaaatct
    56521 tttttagttc tttgtagtta acgattacta cacccttact ttgcgtcatc aagttcgcat
    56581 tttctgcagt ttcatcaatc tcaacaacag cggaattttt caattgcttt gaaatcgttc
    56641 cagtttgcga cttgccgtga attgtgaaca ttacgtgagt attgatcttg aatttttcac
    56701 caatttccga tggtacgata gttgtatcaa attttccaaa agttgccatt ttcataaaac
    56761 ctgcctttca catcaattat accacgtttt atgaaaaata tcatgaaatt aaactaattt
    56821 tccatcggac tctttacaat cagccaaata gagtatataa tttcttttgg ataaggccag
    56881 cttagctcag ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt cgatccctat
    56941 agctggcatt aaaagagaag acagatatat caaggatttt taatccattg atacatctgt
    57001 ctttttgttt aaaggaaata aagtatgctt tcttagcaat agagcatgta ttatttctat
    57061 aaaaatttag gagaataaaa cgactagcaa tatagctgtg aaatcaaata tcacatgaac
    57121 tgcccaactt gtaatgatcg agttgctttt aatggcagct tggttgaata acaatcttgc
    57181 caccccttgt atcaaaagga tgtgtgctac agtgtgtaaa atatttccat tatcataagt
    57241 actgtaatgg ataaatccaa agatgattgc tgacaagaaa ctagcgacag gaatgctgaa
    57301 cttactagaa aaaatagcga gaaaatacac tgagaacaat tcttctccaa gtatcatgat
    57361 aggcaaaata ataaataata acgggctgaa agcctcattt attggattgc ctgttaaacc
    57421 ccatttcaaa atttgttgca aaaataccga gactgcggca ctgattacta cattgataaa
    57481 aaaatatttg gctatatttt tgacaggagc gacaggtttc gagaacattt ttttcgtatc
    57541 ttttttacca aaaacaagtg taaatgctat aaatccagtg atagttaaag aaaaaatgct
    57601 aataatggat ggcgcaaata gcaaccctaa caaaccgagt acaacgacaa tagtgcccaa
    57661 tatccctttt ttaagttcta ttttttttcc taaaatgtta ttcatgataa taactcctac
    57721 tattcagttt ttgaa
//
LOCUS       DAZKCZ010000007        53152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000007 DAZKCZ010000000
VERSION     DAZKCZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.007"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..1132)
                     /gene="recA"
                     /locus_tag="ACG8KN_000342"
     CDS             complement(83..1132)
                     /gene="recA"
                     /locus_tag="ACG8KN_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9187430.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1241..2482)
                     /locus_tag="ACG8KN_000343"
     CDS             complement(1241..2482)
                     /locus_tag="ACG8KN_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFP9187431.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2722..3300)
                     /gene="pgsA"
                     /locus_tag="ACG8KN_000344"
     CDS             complement(2722..3300)
                     /gene="pgsA"
                     /locus_tag="ACG8KN_000344"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9187432.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3509..4432)
                     /locus_tag="ACG8KN_000345"
     CDS             complement(3509..4432)
                     /locus_tag="ACG8KN_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFP9187433.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5171..6457)
                     /locus_tag="ACG8KN_000346"
     CDS             complement(5171..6457)
                     /locus_tag="ACG8KN_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9187434.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6450..7718)
                     /locus_tag="ACG8KN_000347"
     CDS             complement(6450..7718)
                     /locus_tag="ACG8KN_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9187435.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7970..8431
                     /gene="mscL"
                     /locus_tag="ACG8KN_000348"
     CDS             7970..8431
                     /gene="mscL"
                     /locus_tag="ACG8KN_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9187436.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8490..8936)
                     /locus_tag="ACG8KN_000349"
     CDS             complement(8490..8936)
                     /locus_tag="ACG8KN_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9187437.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9029..9274)
                     /locus_tag="ACG8KN_000350"
     CDS             complement(9029..9274)
                     /locus_tag="ACG8KN_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFP9187438.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9389..9967)
                     /locus_tag="ACG8KN_000351"
     CDS             complement(9389..9967)
                     /locus_tag="ACG8KN_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9187439.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10044..10574)
                     /locus_tag="ACG8KN_000352"
     CDS             complement(10044..10574)
                     /locus_tag="ACG8KN_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFP9187440.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10578..11438)
                     /gene="rlmB"
                     /locus_tag="ACG8KN_000353"
     CDS             complement(10578..11438)
                     /gene="rlmB"
                     /locus_tag="ACG8KN_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9187441.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11431..11826)
                     /locus_tag="ACG8KN_000354"
     CDS             complement(11431..11826)
                     /locus_tag="ACG8KN_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFP9187442.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11823..13235)
                     /gene="cysS"
                     /locus_tag="ACG8KN_000355"
     CDS             complement(11823..13235)
                     /gene="cysS"
                     /locus_tag="ACG8KN_000355"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9187443.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13232..13768)
                     /gene="epsC"
                     /locus_tag="ACG8KN_000356"
     CDS             complement(13232..13768)
                     /gene="epsC"
                     /locus_tag="ACG8KN_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFP9187444.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13854..14093)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14090..15550)
                     /gene="gltX"
                     /locus_tag="ACG8KN_000357"
     CDS             complement(14090..15550)
                     /gene="gltX"
                     /locus_tag="ACG8KN_000357"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9187445.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15720..16877)
                     /locus_tag="ACG8KN_000358"
     CDS             complement(15720..16877)
                     /locus_tag="ACG8KN_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFP9187446.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16911..18281)
                     /gene="radA"
                     /locus_tag="ACG8KN_000359"
     CDS             complement(16911..18281)
                     /gene="radA"
                     /locus_tag="ACG8KN_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9187447.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18344..18871)
                     /locus_tag="ACG8KN_000360"
     CDS             complement(18344..18871)
                     /locus_tag="ACG8KN_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9187448.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19101..19907
                     /gene="proB"
                     /locus_tag="ACG8KN_000361"
     CDS             19101..19907
                     /gene="proB"
                     /locus_tag="ACG8KN_000361"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9187449.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19904..21151
                     /locus_tag="ACG8KN_000362"
     CDS             19904..21151
                     /locus_tag="ACG8KN_000362"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9187450.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            21227..21571
                     /locus_tag="ACG8KN_000363"
     CDS             21227..21571
                     /locus_tag="ACG8KN_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187451.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(21688..22014)
                     /locus_tag="ACG8KN_000364"
     CDS             complement(21688..22014)
                     /locus_tag="ACG8KN_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187452.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(22387..23588)
                     /locus_tag="ACG8KN_000365"
                     /pseudo
     CDS             complement(22387..23588)
                     /locus_tag="ACG8KN_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            23705..24220
                     /locus_tag="ACG8KN_000366"
     CDS             23705..24220
                     /locus_tag="ACG8KN_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9187453.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(24419..24865)
                     /locus_tag="ACG8KN_000367"
     CDS             complement(24419..24865)
                     /locus_tag="ACG8KN_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9187454.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(24912..26390)
                     /gene="msr(C)"
                     /locus_tag="ACG8KN_000368"
     CDS             complement(24912..26390)
                     /gene="msr(C)"
                     /locus_tag="ACG8KN_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9187455.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(26744..27373)
                     /locus_tag="ACG8KN_000369"
     CDS             complement(26744..27373)
                     /locus_tag="ACG8KN_000369"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9187456.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(27357..27821)
                     /locus_tag="ACG8KN_000370"
     CDS             complement(27357..27821)
                     /locus_tag="ACG8KN_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9187457.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(28308..29576)
                     /locus_tag="ACG8KN_000371"
     CDS             complement(28308..29576)
                     /locus_tag="ACG8KN_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9187458.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(29807..30544)
                     /locus_tag="ACG8KN_000372"
     CDS             complement(29807..30544)
                     /locus_tag="ACG8KN_000372"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9187459.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(30611..31180)
                     /locus_tag="ACG8KN_000373"
     CDS             complement(30611..31180)
                     /locus_tag="ACG8KN_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP9187460.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            31308..32180
                     /locus_tag="ACG8KN_000374"
     CDS             31308..32180
                     /locus_tag="ACG8KN_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9187461.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            32522..33394
                     /locus_tag="ACG8KN_000375"
     CDS             32522..33394
                     /locus_tag="ACG8KN_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9187462.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            33391..35349
                     /gene="nagE"
                     /locus_tag="ACG8KN_000376"
     CDS             33391..35349
                     /gene="nagE"
                     /locus_tag="ACG8KN_000376"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFP9187463.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(35600..36451)
                     /locus_tag="ACG8KN_000377"
     CDS             complement(35600..36451)
                     /locus_tag="ACG8KN_000377"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9187464.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            36574..37041
                     /locus_tag="ACG8KN_000378"
     CDS             36574..37041
                     /locus_tag="ACG8KN_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFP9187465.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(37183..37665)
                     /locus_tag="ACG8KN_000379"
     CDS             complement(37183..37665)
                     /locus_tag="ACG8KN_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9187466.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(37765..38628)
                     /locus_tag="ACG8KN_000380"
     CDS             complement(37765..38628)
                     /locus_tag="ACG8KN_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9187467.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            39226..40074
                     /locus_tag="ACG8KN_000381"
     CDS             39226..40074
                     /locus_tag="ACG8KN_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP9187468.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(40245..40586)
                     /locus_tag="ACG8KN_000382"
     CDS             complement(40245..40586)
                     /locus_tag="ACG8KN_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP9187469.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            40672..41379
                     /locus_tag="ACG8KN_000383"
     CDS             40672..41379
                     /locus_tag="ACG8KN_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9187470.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            41366..41689
                     /locus_tag="ACG8KN_000384"
     CDS             41366..41689
                     /locus_tag="ACG8KN_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9187471.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(41778..42815)
                     /locus_tag="ACG8KN_000385"
     CDS             complement(41778..42815)
                     /locus_tag="ACG8KN_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9187472.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(43262..44245)
                     /locus_tag="ACG8KN_000386"
     CDS             complement(43262..44245)
                     /locus_tag="ACG8KN_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFP9187473.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(44661..45431)
                     /locus_tag="ACG8KN_000387"
     CDS             complement(44661..45431)
                     /locus_tag="ACG8KN_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9187474.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(45424..46359)
                     /locus_tag="ACG8KN_000388"
     CDS             complement(45424..46359)
                     /locus_tag="ACG8KN_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187475.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(46953..48602)
                     /locus_tag="ACG8KN_000389"
     CDS             complement(46953..48602)
                     /locus_tag="ACG8KN_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9187476.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(48797..49693)
                     /locus_tag="ACG8KN_000390"
     CDS             complement(48797..49693)
                     /locus_tag="ACG8KN_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9187477.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(49686..50366)
                     /locus_tag="ACG8KN_000391"
     CDS             complement(49686..50366)
                     /locus_tag="ACG8KN_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9187478.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(50367..51149)
                     /locus_tag="ACG8KN_000392"
     CDS             complement(50367..51149)
                     /locus_tag="ACG8KN_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9187479.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(51142..52059)
                     /locus_tag="ACG8KN_000393"
     CDS             complement(51142..52059)
                     /locus_tag="ACG8KN_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9187480.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(52062..52346)
                     /locus_tag="ACG8KN_000394"
     CDS             complement(52062..52346)
                     /locus_tag="ACG8KN_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187481.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT        15239 a        11330 c         9500 g        17083 t
ORIGIN      
        1 cgatttcttt attcaataaa aaaacggcag gtcaaggaga gtacctactc ttcagaccag
       61 ccgtttttgt ttatcataat ttttattctt ctactaaagg caattcttct tgtccttttt
      121 catcttctgg gacagcgact tcttcaccaa taccataagc tgctctaact agagcagaaa
      181 cttctgccat catttcagga tgattagcca tatagatttt agcgttttcc cgtccttgac
      241 caatccgatc ttctttataa gaataccaag caccgctttt atcaacaatg tctttttcta
      301 ctgccatgtc tagaagttct ccttcttgtg agatccctaa accatacatg acatctactt
      361 cagcgatttt gaaaggaggt gctactttgt tttttacgac tttgatcttt gtacggttac
      421 caacaatatc tgtaccttgt ttcaattgtt ctgcacgacg tacttccaaa cggatcgttg
      481 cgtagaattt caatgcgcgt cctccaggag tgatttctgg attcccaaac atcacgccta
      541 ctttttcacg gatttggttg atgaaaatag cgattgtttt tgttttgttg atcgaaccag
      601 agagtttacg caatgcttga gacatcaaac gtgcttgtaa cccgacatgt gagtcaccca
      661 tttcgccgtc gatttctgct cgtggaacta aagcagcaac tgaatcgact actacgatat
      721 ctacagcccc acttgatact aaagcatcag cgatctctag accttgttct cctgtgtcag
      781 gctgtgaaag aagtagttca tcgatattca cacctaattt ttgtgcatat tgcggatcta
      841 acgcatgctc agcatcaatg aaagcggccg ttccgccatt tttttgtact tctgcaatag
      901 cgtgtagtgc aactgttgtt ttacctgaac tttcaggtcc atatacttcg atgatacgtc
      961 cacgggggta accgccgaca cctaaagcga catctaacgc aagagagcca cttggaattg
     1021 tagagatttg ttggtcgatt ttttcgccca atttcataat agagccttta ccatagctct
     1081 tttcaatttt tttcagtgca gcatctaagg ctgctttacg atcatctgcc aatacgataa
     1141 tcctccttat atttacaaat cagagcaaca tttcattatc cttatcatat cttttttagt
     1201 gaaaaaaagc aagaaaaaac cgaacaaata ttcgcattta tcattttctt aaaagctgtt
     1261 ttctcaaaag atcaaatcct tgcataaccg cattatatct gatagcagca cgatctctgg
     1321 caaagtgata tttttccgaa accacgccgt ctttagatga aatagcgatc catactgttc
     1381 ctgcagattg accttctaac gtgtccggtc cagccactcc ggtgaaagct acactgtaat
     1441 ctgttcttgc tatctcacgt gcttgaatgg ccatctgctc cacacattct ttgctgactg
     1501 ttccatattc tgctaatagc tccggatcaa tctccagaaa accagctttt gtttggagcg
     1561 agtaggtaac gaatccgcct ggaaatactt cagatacacc agcaatgtcg cctaatgcgg
     1621 cttggaaggc gccggctgtc aaactttccg ctgctgttac agtctgcttg ttttctttca
     1681 gtagttccac tacgactttt gctagcgaat tatcatcgcc gtatccataa aaatattccc
     1741 ctactcgttc ttggattttt tcttctaatg ctaaaagagc ttgattccct gcctgaacat
     1801 catttgtttt cactgtcagt cgtaatgtca cttcatttgg tttggcatat ggtgcgatcg
     1861 ttggattgat ctgtgtctca atcagatctt ttagctcagt gaccagtcgc gattcaccaa
     1921 tcccataaaa gcgcaaaact ttagaaatta gtttttcttc agaaggaaaa cgttcctcta
     1981 gtaaaggacg tacttgttcc acaaacattg gtttcaactc attaggcgga ccgggaagaa
     2041 ggatatacgc atgtgtatct gtttgataaa aagtccctaa agcaagccct gtacgatttg
     2101 gtagaggaac cccgccttcg ataatctggg actgttgcag attgtttttg gtcatttttc
     2161 gatgggttgt ttcaaaatat gccaataatt ttttgtatcc ttccgtattt tgtatgagag
     2221 acttaccgag gtgagccgca gttgcttctt ttgtcaggtc atcttctgtc ggtcctaatc
     2281 cgccacataa gagtatgagt tcacttcttg tttcagctag agcaatcaac tcttctaaac
     2341 gctgctgatt atctcctaca actgtttgat aataaacttc gatacctaga tcagctagct
     2401 gttctgacaa gaaagtggca ttcgtattca cgacttgtcc tagtaaaagt tctgttccga
     2461 cagctattat ttctgctttc attacttccc gctcccttat atagtatacg ctgtattttt
     2521 atattttttt atttaaaaag agggcaagga gctgtgacaa aaatcttgtc acagctcctt
     2581 gttccaaata aacagtgttc tcctgccaaa acctcggtca taagtcacaa tatacataaa
     2641 acatgaaaag ctgttttttg aaattatccc ttgtgactca gagttagacg gcaggctcac
     2701 aacctctcca ctaaacgata cttacattga tcctttgaac acaccgctgt ttttgatgaa
     2761 gtaatccaca cctgaataga tagtaaagat cagacaagca taaagcatga tttgatctac
     2821 tggaataccg atcaggctga atgggatatt attgataaac agcaagataa tagcaaccat
     2881 ttgtgtagcc gtcttgactt ttcccggcca agctgcagcc atgacttctc catgttcgac
     2941 aagtagtaaa cgcaagcctg tgacggctaa ttcacgacag acaatgatcg ctactaccca
     3001 accaggggcc tttccttggt caaccaacat gataaaagca gtcataacta gcattttatc
     3061 tgccaacggg tccgcaaact tgccaaaatt agtgacaagg cctcgcgcac gggcaatttt
     3121 tccatctaac cagtctgtga tactagctaa agcaaaaata actgcaccta ctagctgtgt
     3181 gacagccagt gtggtgtctc ctacgttaaa ttgtccccaa tctaaaggta aacctacaac
     3241 caaaatgaaa atcgggatca tacaaattct aagtacagtt aatttatttg gtaaattcac
     3301 actatcgact cctttcttcc ctattctacg tgactatcag cgaagtttcc accgatatta
     3361 tttacttttc caaatgaatt tattcaaagg agtattttta ttgaaattga tctttttgaa
     3421 ttaacgaaga aggaccgcga cacagatttg tcacgatcct tcttttttat acctacaagt
     3481 aaacagatgc gtaaactaat ttatacgatc attctgcgta ttgaagagta aggttcacat
     3541 tttttggact ctgattgttt tcccctggat taactggtac ggattctcca ttcgctttga
     3601 tcgttgcatt gcttgctgca ccaatcgtga tggtcgcatt tgttgctgtt tccggtaaca
     3661 cggtactctg tgtttcattt gctgccagtg tcccttgata aacataggca ttgtctacta
     3721 gcacacctaa ccatacacga ttgacagcag taaattcaag ggttacaggc ttggtagcgt
     3781 ctgtgatatt tactgcgatg gcactgccag aatcttctcc tgtagctatt gccattttct
     3841 tttcttcact tgattttgtt gttgattcta ctgtagaaga ctctttggtc tgcgtgcttt
     3901 gctctgagga agaggactcc tcgactgatc cttctacttt aagcgaagaa gctgtgtctc
     3961 cgattattgg atcagcttga cgatcttgcc aagtcatata gcccacgaca acgacaatcg
     4021 ccaaagcaac caatcctaat aaaatgacag gaagacttgt ccaaaagcga cttggatgcg
     4081 attcttcctg atgcattgct tttctagaag tacttactgt ttcaggctgc ggacgttcag
     4141 ggaccgatcc tgtcaatttg cttttcccat caaaaacatc gactaaatgg tctccatctt
     4201 ctcctacagc ttcagcgtat tgccgaataa atgcccgcac gtaaaattta ccaggcaatt
     4261 ggtcaaaatc atcattgtcg attgcttcca aataacgttt ttgaattttt gtaatctgct
     4321 gtaattcatc taaagatatt tttttattca ggcgtgcttg ccgtaatttt tctccgatgg
     4381 tttcaaaagc cactcgttca tcttctccag tctgtttgat tctccgaagc atctgctgtt
     4441 gcaacgaaag acgttatgtt tgggtgctgt gacaaaagtc atgtcacagc tccttttttc
     4501 cgaataaaca ttgttctcct gccaactcct cggtcacaag tcacaatatc caataaatat
     4561 aagaagctgt tttttgaact tgttccttgt gactcggagt taaacggcag gctcacgacc
     4621 tctttcttaa acggtgttca atcagctgtt cctcggtatt agctcaaaaa ccggcaaaat
     4681 ataaggagat atttttccgt atgtttctct aatactcagg tcaaaacgct gatttcacaa
     4741 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagtcca accaagttaa
     4801 aaacattaag aagctgtttt tacttggctg ttcttattac ttgatgcagg gcagcttttt
     4861 cacaacctct tggtacacgg tgttccagaa gtagcttctt cggcaataag ccgaaatatc
     4921 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
     4981 tgtcacaacc tctttgatta cttcgtttga ttttatttta atataagttg attaaaagag
     5041 agcatgattt gttttatttt ctcactaatt tttccaacac aactaagaat accataaaag
     5101 ctgattttgg aggaggttcc tactacaagt ctaaaacgaa atttaagact tttcctcata
     5161 aaatactggt ttattttggt ctcatgtaaa actcgctgaa ggcctcttca cttataaatg
     5221 cttccccagc tgccagtacg tctttcattt ggatcgaatc gatgatttca ggaagatcaa
     5281 ataaagttcg atcgccaaat aaactttgcg taaattgatt ggcaatatat tcgatcgaat
     5341 ttaatgattg gaaatattgt ccaagcattt tcttcttcag caaatcgaga tttgtttctg
     5401 acacttccgg atcatcagcg aatccaagaa tgatttcttt gactttttct gctgctttct
     5461 ctggttcgtc cgtatctccg ctgaagtccg caaagtgaaa ttcacgatcc aagctgaatt
     5521 caaaaccaaa gctgtcatcg ataattcctt gattgtacat cgctagatag tttcgagaag
     5581 tattgcctaa aatcatttgg aacaacagat tgattgctgt tttatagcgt aacagttctg
     5641 ttccttcttg cggcaatgtg tctaatcctt taataccaag tacaaattta tctcgtgtaa
     5701 tggctgcttc caatgcactt tgtttgatga tttcttttgt gttttcaggg aaataacgta
     5761 caatttcttg ctttggaggg aaattttttg cttcttggtt ttcccgaata agtttcatca
     5821 atttctcagg ctccatctta ccaactacaa ataaaaccat attgcttggc tgataaaacg
     5881 tccgatagca cgtatacaag tcttgagctg tgattttgtc gatactttca accgtgcccg
     5941 caatatcgat atgcaatggg tgtgtaggat acaagttatt tagtatgcca aaaaacatcc
     6001 gccagttagg gtcgtcttca tacatttgga tttcctgacc gatgattcct ttttccttgt
     6061 ttacagtttc ttctgtgaag taaggtgctt gtacaaaatc gatcagagtc gtcagattct
     6121 tttctacctg atccgtcgta gaaaacagat agctggtttt cgtaaaactt gtgaaagcat
     6181 tggcggaagc tccttgtttc ccaaataatt gaaagacatc accatcttct ttttcaaaca
     6241 acttatgttc taaaaaatgg gcaattccat ctggaacttt caccttttct ttttcaccat
     6301 agggaataaa ttcgttatcg atcgaaccat aattcgtact gaacaaacca tatgtcttgt
     6361 gatagtcatt tttaggcaac aaatacacgg tcaaaccgtt tggcagtact tcatgataaa
     6421 gtgtttcatt gatttgctca tattctgttt tattcatttg catttcctcc tgctaaaaag
     6481 aagattgcct gcaattcgat ttgctcagct acttgttgta tctctttgat cgttacgctc
     6541 tccatacgtt tcaaccattc ttcatcagat aattttgttt ccggcagcca gctgtccaaa
     6601 aaagaagctt cgattgccgc ttgcggagag tctagagaaa gcagatattg gtttctcagc
     6661 attgcttttg tctgagacag ttccaactct gttatttcac catttcgcaa actttctaat
     6721 tgctcgtgaa tcagatgaag tacttgattt cggttctttt catcgatacc ggtttggaca
     6781 ctcatgaatc ctcggaacgt atccacgcta ctagaggcat aataagctag actttctttc
     6841 tctctgacat tcatgaaaag tttagagtga ggaaaaccgc caaataaacc gttaaagacc
     6901 attagcgcaa aacgttctgg ttcatcgtaa tacacatttg tctgataagc taggttcagc
     6961 tttgcttgaa cgattggctc acgtacttgt cgttccttca cgatattcac ttgaggttgg
     7021 gtataaaaca gatctggata aactgtttcc cgcgactcaa atggtaattg cccaactgct
     7081 tcagctgctt tgtccggatc aacatcgccg acaacaaaaa tgtcgatttg atcatttgcc
     7141 atcatttctt gatagtagga cgctagcgaa ctagcagtga tcttgtccaa atcttcgact
     7201 acgcccaacc ctgggatctt ttgttccggg ctgttttcaa aataaagttc ttgcaaggca
     7261 taggaagcaa aagtttgttt atcttccttc aaactttcca aatatagacg cagattgttc
     7321 ttttccaagt caaacgtcaa ctgatcaaac tgatgatttt taatatttgg ataaaataaa
     7381 acctctttta aaaaatctac cgcttgagaa aaaaggtttg gatcattgac atattcacca
     7441 ttcacaaaac tgatccctgc gtttagccaa tgtaaatttc ctttttttct aactgataat
     7501 ccaaagcttg cgccataaag ctctgcaagt tgcgcactta actttgtctg atccggataa
     7561 ttcaaactat ttgtttccaa catgctgctg agaagtgaac gtttcgtgat gacttcttgt
     7621 tgcagacgcg cagtaaaacg gataaaaatc cggatcgttt tgtattgttt tgttggtaag
     7681 acatgaagat tcacgccttc tgccaattga tacaccatat ttatatgctc cttttcacag
     7741 ttgattgccc gcttactctt ttaatgggtc aacctgcctt ttttacttat tcctctgctt
     7801 gaaatttata agttcaggca attcgccgtt ttttctaaat gatgttacac tttatagtat
     7861 agcatagatg ttttttatca aaagaaacag acggctaaaa gcgtatattg tttttttatt
     7921 ttttattttt cgatatacta aagacaaata aaagaaggga gggtcattca tgatcaaaga
     7981 attcaaagag ttcatcatgc gtggagatgt cttggatctt gctgtcggcg ttgttattgg
     8041 tagtgcgttt acaagcatcg taaaccaagt ggtgcaaggc ttgatcacac cactaatagg
     8101 ttggtttgtc gcaatgatca caggtacttc tgatttagaa ggagcacttt caattcttga
     8161 ctggtcgccg acaaaaggcg tcacatttgc gtttggtaac gttattagtg ctatcattac
     8221 atttctgatc actggttttg tcttattctt agttgtcaaa gcggcaaacc gtgcgaagaa
     8281 tttacgctcc aaagaagagc aagtggaagt ggaagaaact cctactgcag aagattatct
     8341 tagtgacata cgcgatcttc ttgccaaaca agtggcagaa gacaatacac atccagtaga
     8401 gcaaacaaat ctttccgata cttctaaata ataaagaatc taaaaaaaag aaagccgatt
     8461 ttcagtgtcg gctttctttt tttagattct taacttgcta cttgttcctt tttcatgtcg
     8521 atatgtggaa tatcatcttc caaatacaca tctgagattg ccttgaatcc aaacgactca
     8581 taaaaagctt gtaggtattc ttgtgcgccg atcacgattg gtttttctgg aaaatttttc
     8641 tggatcatgt taatcgtttc ttgaacaatc tttcttccta aaccatcttt acggttgtcc
     8701 tgatgaacaa ggacacgtcc aaaatggata ctctccgctt cttcatatac tcgcgtgtag
     8761 gcaaggatct tcccatcagt atcttgaaaa aacgtatgcc aagcttgtcc atcaatgtca
     8821 tcaacttctt gataaggaca ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg
     8881 atcgcgtaaa actctgctgt tgacaattct tcaaatttct ttacctgata ttccactaag
     8941 caaacgcccc ctgctttttt attcttagca taccgattcg cacacaaaaa agcaaacgaa
     9001 ccttacgaat ctcaggctcg tttgcttttt aaactgcttc agttaaaaat tcgatctcaa
     9061 ctgtccgagt aagtacatct gaatagctgt aagatactcg ttcaaaagaa ttttcttcag
     9121 gatccaaatc aacaacgaat actgaaggat aggtttctgt taaaacgccg cggcgttctg
     9181 tttgacgctt tcttcctgtc tgagcaacca acatgattgg gctgccgata cgaccttcta
     9241 aatctttctt gattgaagct aaagttgttg gcatacagtt cacctctact atcaactata
     9301 ccacatattt ttaaaatttt caagtttacc ataaaaaaaa aagaaatgca ataaaaataa
     9361 ataagaagct cattaagatt tttctaaatt attcctcatt ataaaatttc aagtgttttt
     9421 ttattttgag ttttactctc ccgtaagcgc tcattacttg cttgtgatcc attgctaaac
     9481 attcagccat gtctacaaaa tcaacgcctt gcatataata atgaaaaatt ttagcttcta
     9541 tttttgaaaa ctggatttcg caattttcga ttgcttcttt taataaaaga tgctgaatcg
     9601 ctaaaggttc ttgcaatgtg atctctgcta aaaagtctgg tccttctgtc tctatttttt
     9661 ggtcgattga gacttcatct ttttgatcac gacgcttcag cgcgttctgc ttcctcaaca
     9721 gagaatagat ttgattgctt ataatgcttt tataataact tccaaagcta acacctttgc
     9781 ttttttcgta agttctcaat gcctgatata aagcgatcct tgcttcttgt tcccaatcct
     9841 cagaaacata atttcttaaa taataggtgt ttttcaaacg tagtaaaagt ggaaaatatt
     9901 tttgatacag ggcagcaaat gcttcttcgt caccattacg tgcagcgcgc aataactcct
     9961 cagacataaa ataattcctc cataaattcg tttatggagg aatcttatca tcaatgattc
    10021 atcgaactga ttagaacgat atgttattta ttttttctca ttaaatcgtc caatttttgg
    10081 gacaattttt ttaactgctc ttcattccat ggagaattac gtcggaaatc aataaaatgc
    10141 atatctttat tttcttgggc tattgttttt tccgtttttt ttatcgtctt atataattca
    10201 taagcagaag ttcgtaatgc tccttgggaa aagatcatcc actgttctgc taaatcgctt
    10261 gtcgccactg tcacttgtgt caaacggtca ttcagctgac cagcgattct ctcgatataa
    10321 ctgtcagccg tttcgtcttc tttcgtaaaa acaacatcca actggtattt cttataattt
    10381 tgttggatgc ccggaactag ttgggcatcg aagacaacga tgacttccat tccttcatat
    10441 ttcgcataat ttgataaacg atgtaaaaga gcttctcttg catccgccat tttgtcctgc
    10501 tttttaagct ggacaagttc cggccacgaa ccaatcatgt tgtagccgtc tacaatcaac
    10561 agctgttttt tcatggtcta ctccccctga cgttgggaaa aagcctgata cataagcaaa
    10621 ccagcagcaa cactggcatt taagctctgg acgtgcccgg tcatcgggat cgtcagcaat
    10681 tcatccattt cttttttcaa tcctggactc attcctttgc cttcattacc aatgactaaa
    10741 gcaattgatc ctttggcatt ccatttttgg aagtttgtgc cggtcatatc tgtgccaaat
    10801 acccaaaatc ctgtttcttt gagctgttgc acagcttgta ccaaattcgt cacgcgtgca
    10861 acagggacat gttcgactgc cccagtggaa gtctttgtta cgataggtgt gattccaact
    10921 gctcggtgtt ttggtatgat cacaccatcg acgccacttg cgtctgctgt ccgtaaaatc
    10981 gacccaaaat tgtgtggatc ttcgatgcta tccaaaatca aaaagaaggg gtcttctttt
    11041 ttctcttttg ttcgttcaat cagttggtca agggataaat attcataagg tgtaatcgca
    11101 agaacgactc cttgatgcac gccgtgatcg ctcaaagttt ctaatttttg tttaggaacc
    11161 catttgactg gcacagcttg ttcacgtgcg atttctttca gttcttctac tttttctcct
    11221 cgtgaatctt cttgaagaaa gagtttattc cctcgccctt gttgtagcgc ttctactgcg
    11281 gcatggtgcc caaagacgaa attatcttct aatgcttctt cattttgatc agaaactgac
    11341 tgatttcgtc cagtttgctt tttatttgaa tatctatttt gttttttgtc ttcaaatcca
    11401 cgtttcgatt gatgtttgtt tgttctttct ttacccattt ttttcaccaa ctttctgaat
    11461 acaccagcgg atcaattctt ccatccgctc ggtttgtttt gtcaaatgca gatagcccat
    11521 gagcgcttcg aaccctgttg cgacccgata cgtcgtaaca tcagcatttt ttgcgctcgt
    11581 atggctttta gcatttcttc ctctgcgata ataaagttct tcttcttctg tcaaaagatg
    11641 ttcttccagc atttgctgaa tcaaaaaggc ttgtgcattc gcagaaacat aattcgttgc
    11701 cgcacgatgc agcttattgg gttttgtttc tccttgatca atcaggtggt cacgaacata
    11761 gatttcgtaa atcgcatctc caacataagc caaggctaaa ccgttcaatt gtgtgtaatc
    11821 tctcatgctt ctcttctcca tcgggttcct tgcggtgtat cttctaataa gatccctttt
    11881 tctttcaaca aatcacgaat ttcgtcgctt cggacaaaat cacgattgct tcttgcttga
    11941 tttcgttctt cgatcaattg ttcaatctct tcatctaata actcttctgc tgtaaataaa
    12001 ataccgaaga tcgaaagcca ttgcgagaat aaagcttctc cttgtgccaa gacagcggca
    12061 gaaactttcg gctggtctag ataagtgttc agccacttag caagctcata gaccactgtg
    12121 atcccatttg ctgcattaaa atcatcgtcc atttcttcga tgaatcgctg ttctaaattt
    12181 tctaacaaag cgattttatc cgcatcatct gccaaattct ctgaggcagt ttcttggcgg
    12241 aatttcaaat tgtcaaacgt attcttcagt cgttgtaaat tggtggctgc ttctttcatc
    12301 gtcgtttcac tgtagcggat cggacgacgg tattgtgtgg tcgccatgaa gaaacgcaag
    12361 acttgtggat caacgttttt gatcatttca tgtacagtga tgaaattccc tagagattta
    12421 ctcatttttt catcgtcttc gccaatcgtt acatagccat tgtgcatcca gtaattagcg
    12481 aattttttcc ctgttttcgc ttcactttgt gcgatttcat tttcatgatg aggaaattct
    12541 aaatcttgcc cgccaccatg gatatcgatc gtttctccta aatgttttgt tgccataacg
    12601 gaacattcaa tatgccaacc gggacgtcct ttgccccatg gtgaatccca agaaatctcg
    12661 ccaggttttg cttgtttcca taaagcgaaa tctaatgggt cttctttttg ctgctgttct
    12721 tcccctgtac gctgacttgc tccgacttcc agttcatcga tggattggtc acttaatttc
    12781 ccataatttt caaatttacg ggtacggtaa tacacatcac ctgaagattc ataagcatag
    12841 cctttttcaa ccaacacttg aataaatgct aaaatatctg gcatatgatc catgactctt
    12901 ggatgcagtg tagcaggctc gacattcaac gctttcgtat cttcttcaaa tgctcggatg
    12961 aaacgatcag caacttctgg tgctgtaata cctaattcgt ttgctgtacg gatgatttta
    13021 tcgtctacat ccgtgaaatt cgatacgaaa tcgactttat agcctcgata ttcaaaatag
    13081 cgtcgaattg tatcgaaagc aatggtgcta cgagcatttc cgatatggat ataattatac
    13141 acagtcggtc cgcagacata cataccgaca tgtccctttt gtatagggat gaattcttct
    13201 ttttttctgg tcaatgtatt gtagattttt atcattctga tctcctcact ttttctcctt
    13261 tgattcgaac gacttttgct ggtactccta ccgctgtagc gtcgtctgga acatctttca
    13321 acacaacggc acctgcaccg attttggcgt tttttccgat cgttaccggt cctaatatct
    13381 gcgcattcgc agagagcatg gctccttgtt taacagttgg gtgtctttta cccgtttctt
    13441 ttcctgtgcc gcctaatgtt acgccatgga ataaaatcac atcgtcctcg atttctgcgg
    13501 tttcgccaat gacgattccc attccatggt cgatgaacac gccagttcca atcgttgctc
    13561 ctggatggat ctcaacacca gttaggaaac gccagaattg agcattgatc ttcgccaata
    13621 aaaagagccg gtgctgatat aaaaaatgag agaaacgatg ccaaaacaag gcatgaaaac
    13681 cagggtatgt caaaacgatc tcagctgtcg aacgtgcagc cggatcgttt ctctttgctg
    13741 catcaatcgc tcgggtaaac caactcatga ctttccactc ctttcgtaag aattttatgt
    13801 aggaaatcat cttcttataa agaaaagaag cgccttttaa ataagtttcc ttatcaaaag
    13861 acgctttccg cgtggttcca cttttcttca taatcaatga ttatctctga cagctaacgc
    13921 ctgttatacg ttcttggcta ctttgtttcc tataaaacag tttttcctgt tttctgtgtt
    13981 tcgccaagac cactcccaga ggcatttcgg caaccattca ttgatacctt gcaccacccg
    14041 gtatctctct tgtaatgatc agctgcttac ttttttctgt tcatcgtgtt tagttcagta
    14101 cttgatttaa atgtgctaat gatttttcac gaccaagcaa ttcgatcgtt tcaggcaatt
    14161 ctggaccatg catttgtcct gatacagcta cacggattgg catgaaaaga ttttttcctt
    14221 ttacgccagt ttctttttga acagctttga tggcagcttt gacagttgga gcatctacta
    14281 cttccatttg ttctagctgt gctttgaatg cgtggagaac agtaggaact gtttcaccag
    14341 caagtacttc ttttgctgct tcattcaata caggatgttc attgaaaaat agcgtagaca
    14401 gatcaacgat ttctgcagca tagctcattt gaggttgata caggctaacg atcttcttca
    14461 cccattcgat tgtcttttca tctgggtttt ctgctacacg tccatcagcg attagataag
    14521 gcagacacat gtcagtaaat gcatctagat ccattgcttt gatgtaatgg ttgtttaccc
    14581 attgcagttt tttcgcatcg aaagctgcgg gtgatttact taggcgtttt ggatcgaaca
    14641 ttttgatcaa ttcttggtgt tcgaagattt cgtcttcgcc cacaggtgac cagcccaata
    14701 atgcaataaa gttgaacatt gcatctggta gatagcctaa ttcgcgatat tgttcgataa
    14761 attgcaagat tgattcgtca cgtttgctta gttttttacc tgtttcactg ttgatgatca
    14821 atgtcatatg tccgaaggtt ggtggtgtcc agccaaatgc ttcatagatc atcagttgtt
    14881 ttggtgtatt ggcaatatgg tcatctccac gtaaaacgtg agtgattttc atcaaatgat
    14941 catcgaccgc aacagcaaaa ttataagttg gcataccatc gcgtttttgt atgacaaagt
    15001 cgccaccgat attatcggat tcgaacgaaa tagcgccttt caccatatct gtaaatgcgt
    15061 attctgtatt gcgaggcaca cggaaacgta caacagattc tagaccttgt gcttcttttt
    15121 ctgcttgttc ttcaggtgtc aagttcgcac atgttcctgc gtaatgaggc atttctcctc
    15181 ttgcgcgttg tgcttcccgt tctgcttcta attcttcttc tgtgcaatag catttgtatg
    15241 ctcgattgct agcaagcagc tgatcaatca gcggttgata gatttcttta cgttcagatt
    15301 gacggtaagg gccgtattct ccaggatttt caggagattc atcccaattc attcccaacc
    15361 aagcaaggtt ttccaactgg cttttttcgc catcttcgat attacgtttt tggtcagtat
    15421 cctcaatacg aataataaag tctccatcat tgtggcgggc aaacaaataa ttgaataatg
    15481 cagttcttgc atttccaata tgcaggtgcc cagttggact tggtgcatag cgtacgcgta
    15541 ctttcgtcat tttcttcctc ttctttcaaa ctagactttg actaacagca aaaattccgt
    15601 cagttacggc ttctcaaata tcatttattt aaagccactc agcacaaatt ttaccttttc
    15661 taaggtaaat agtaaagcca actggttgaa tttccttacc agttggctga atttcttttt
    15721 tattttgatt gtttagtctt tttctctgtc tcttttttat tgtcgattcc tcgtcctgca
    15781 tgtgcaggtt tagcaaagat catgcgtcca gcagctgttt gaagggcact tgttacaacg
    15841 acttcgatac gttcattcat gtaatgctgt ccatcttcta cgacaaccat cgtgccgtca
    15901 tcaaggtagg ctactccttg ttggcgttct gtcccggctt ttactactag aacattcatc
    15961 gtttcaccag ggatgacgac tggttttacg gcatttgcca acgcattgat attaagaaca
    16021 ggtacatttt ggaattcaga gactttattc aagttatagt cattggttac aatgacacca
    16081 tctaaaagct tagccaattt gatcaatttg ctgtctactt cactgatatc ttcaaagtct
    16141 ccatcgtaca tttcgacaga tataccctct tctttttgca aggcatttag gatatctaat
    16201 ccgcgacgac cacgcacacg tttcaaacta tcccctgagt ccgcgatgta ttgtagttcg
    16261 taaagaacaa aattagggat catcaaggtt ccttctaaga atccagtctt tgtaatatca
    16321 taaatccgac cgtcaatgat cacgcttgta tccaagattt tatatttatg gaaattttct
    16381 cctgattttc gttctaacag ttgactctca acttgactag ctgtttcttg ctcgattttt
    16441 ttgcttcgtg aaccaaaaac tttcttccat tcttcgattc gtgtcgttcc cattctcaat
    16501 cccagatagc ctaagatgat catcagtaaa atcggcaata cactattgac gaatggaatt
    16561 tccatattgt aaagaggggt cgagatcaca acacctaaag tcaatccgat gatcgcaccg
    16621 atcgcaccga agagaagata agtcaagctg acctcactta gtttttgttc tacttgtttg
    16681 ataatagctg agatatgttt ggccaacagc aaagataata aaaagaaaat aagtgcgcct
    16741 aacaggctgt tggtaaatgt attattcagc catccgtttg actgttgacc gattgcctgc
    16801 caagctgagg gtaaaaatga aatacccaag cttgcgccga caatgatcat cagcaaagta
    16861 atgacacgtt tttgcatcgt ttttcctccg tttcgacaat catttattag ttagcggaac
    16921 acacgcttca acgtttcagc caaagttgct actccaacta tctcgattcc ttttggcgct
    16981 tcccagttac ctaagttgtt tttaggtagg tatacttttg tgaagccgag tttttggact
    17041 tctttgactc gttgttcaat atgactgaca cgtctgattt ctccagtcag tccaatttca
    17101 ccgatgaaac attccgttgg ctgtgttcct ttttctttat agcttgaagc aatactgaca
    17161 gcgatagcta aatcaatcgc tggttcattt aattttaccc cacctgcagc ttttaagtaa
    17221 gcatcttgat tctgaagaag caacccagct cttttttcca gcactgccat gatcagagat
    17281 acccgattga aatcaagacc tgttgttgtt cgtttggcat ttccgaacat agtaggtgtg
    17341 actaatgctt ggatctctac caaaattgga cgtgtccctt ccattgccac aacgatagca
    17401 gagccagttg caccgtctag gcgttcttct aagaaaatct gtgaaggatt agcgacttct
    17461 tccaatccat gctcatgcat ttcaaaaatc ccaatctcat tcgttgagcc aaatcggttt
    17521 tttactgctc gcaaaatccg aaaactatga tgttgttcac cttcaaaata taacaccgta
    17581 tccaccatat gttctaacat tcttgggccg gcgatcgagc cttctttggt aacatgaccg
    17641 acgataaaaa tggctatccc atttgtttta gcgattttga gtagttctgc agtggtttct
    17701 cttacttggc tgacacttcc tgccacactt gtgatgtctg gctgcgtcat cgtttggatg
    17761 gaatcaatga taacataatc aggggagata tgttcgatgg cacgactgat ttcgttcata
    17821 tctgtttcag cgtatagata aaattctgtg ttgatacttc cgaggcgttc cgcgcgtaat
    17881 tttatctgct ctgcgctttc ttcgccggat acatataaga cttttccgcc tatggctgct
    17941 aattgctgag aaacttgtaa cagcaaagtg gatttcccga tacctggatc gccaccaatc
    18001 aagaccatag accctggcac cacacctccg cctaacacac ggttcagttc ttctaatttt
    18061 gttttgatcc gaggttcttt tttagggacg acgtctgcga tttttgttgg ttttgctttt
    18121 tctcctgtca aacttgtccg agttctgcgg tccgttgtat cttgttcgat ttcttcgacc
    18181 atcgtattcc attttccgca atttgggcag cgtcccaaga acttaggcga cacatacccg
    18241 caattttggc aaacaaattg tacttttgat ttttttgcca ttgtaccgcc tccttttatc
    18301 ctaatcatga gacattaaat caagcttaag tatctttaac gttttattta ctggaagaac
    18361 caaatccgcc ttgacgttga gcagtcgttt gatcttgatc tgcttttaaa aacggcaaaa
    18421 agatcccttg gccgatacgc tcgccttttt tgatcactac atccttgaaa ccaaagttgg
    18481 tgaattggaa catgatatgg ccttcatttg cctcattatt gtaataatca ctgtcaatca
    18541 cacctactcc gtttgtcaac actaaaaacc gcttcaacgg attgcttgaa cggttagcaa
    18601 gctgcaaaaa ttcatcttct cccatgtaag ctttgattcc agtaggcacg agggttggtt
    18661 tcaattgttt ttcattttct gatacatctt ttttcaatcc atctgcgact gtttccaacc
    18721 cctttttcca gcctgctttc cagatacttg gaatcacgac ctcttctgct gcttcaaaat
    18781 catagccagc agcatgatgt gtagcgcgct gcggtagatt gatattttga tctttatatt
    18841 tagaaataac ttcaaatcct ctttttctca ccggttttcc tccctaaata aactactcct
    18901 attctaacat atccgaggct gtaaaagact ttctttacaa attcttcaag aaattctgct
    18961 atcttaaagt aaaccattaa ttcgttcttt ttccaaacga ctgcaagttg tacaaaaaaa
    19021 ctatttttta catgacttta ctcgtcaaaa caagtagaat agaacgaaat taatgagtgg
    19081 atatttgatt gaggtgtcac atgcgtaaag aaattaaaaa tgcaaaacga attattgtca
    19141 aagtaggaac aagtacattg atttatccca atggaaatat caatcttgcg gcaatcgatc
    19201 agttagcttt tgtcctttct gatcttagaa atcggggaaa ggaagtgatc ttagtctcat
    19261 caggagctgt aggcgttggt atgcataaat tacatttgtc agaacgtccg actttgattc
    19321 ctgaacagca agctgcggcg gctgttggtc aagccgaact gatgaatatc tataatcaaa
    19381 gattcagtgt atataatcaa cagacggcac agatcctcgt gacaagagac gtggtagatt
    19441 atcctgaaag tcgtaaaaat gtgaggaata cattggaaca gcttttgatt atggacacga
    19501 tccctatcgt gaacgaaaac gatactgtag cagtggatga attagatcat ctgacaaaat
    19561 ttggcgataa tgatcagctg tcagccatca tggcaaaact tgtcgcggca gaccttttga
    19621 tcgtcctttc tgatatcgac gggttttatt cagataatcc gactacaaat cccgaggctc
    19681 agatgtatcg ctatatcgat aaaatcgatg aaaagattct ggcacaagca ggtggtgctg
    19741 gtagcacatt tggaacaggc ggtatgcaga gtaaattaaa agccgcttct aggatatttg
    19801 aagcaaacag cgcaatgatt ttagcaaatg gaaaaaatcc ggcaattatt tttgatattc
    19861 ttgcaggaaa agaagtaggt actttattta aggaggactt gaaatgactg acttgatgca
    19921 attaggacaa caggcaaaag atagcgcgca acaactagca cttatgccta caaaccgcaa
    19981 aaatgagcta cttgttcaaa tggcacaaac catcaaaaag aatcaacaag cgatcattga
    20041 agcaaatcat aaagacttag aaaaagcagt cgaaaacaat atcagtgaaa cgatgcaaga
    20101 ccgcttgcag ctgacacctg agcgaatcaa tgccatggct acagagatcg aaaaaattgc
    20161 ttcgttagac gatcctattg gaaaagtaga tgagatgtgg acgaacaccg acggattgag
    20221 aatcggcaaa aagcgcgtgc cactaggcgt gaccggcatc atttacgaat ctcgtccaaa
    20281 tgtcacgaca gatgcagcta gtctagcatt caaatcaggg aatgccgtta tccttcgcgg
    20341 tggcaaagaa gcattcttct cgaatcaatt attagtccaa ttattgcagc aagtactggt
    20401 cagtgctggc gaatctcctt atgccatcca gtttgtcgat gatacttctc atgaaactgc
    20461 aggacagttg atgcgattga cagaatactt agatgtgctg attcctcgag gcggagcaaa
    20521 attaatccaa cgagtcaaag aacaggcaac ggtacctatc attgaaacag gtacaggaaa
    20581 taatcacgta tatatcgata aagaagcaca gttatctatg gcagtgaaca tcatcgtcaa
    20641 tgcaaaagct tcccgtccat ctgtctgtaa cgcggcagaa acattgctga tccattctga
    20701 gatcgcacct tttttcttgc cagctattga aaaagaactg gtagaacacg gggtctcttt
    20761 acgtgcggat gctcgtgcgt tagaatatct agagacggct gctttagcag aagacacaga
    20821 ttgggatact gaatatctag attatatcct tgctgtcaaa gtggtcgatt ctttggatga
    20881 agcaattgca catatcaatc ggcataatac taaacattca gaaacgatcg tgacagatag
    20941 ttatgcggct agtcaacgtt tcttaaatga agtggacgcg gcggctgtct atgtcaatgc
    21001 ctccactcgt tttacagacg gatcggtatt tggttttggt gcagagattg gtatctctac
    21061 tcaaaagttg catgcccgtg gtccaatggg gttaaatgaa ttaacttcca caaaatatat
    21121 catttatggc gatggacaaa tccgcgaata atgtttcgtg tgaaacatta ttcccgcctt
    21181 ttgccttttt tctgtaaagg ttctatccaa ctagaaaggt gtcaatatgt ttgaaatttt
    21241 aatgattttt tttatttacc ttatttctct gaatatcgct gcttttctgg gagtcggtat
    21301 tttatccctt ttctttcaat tcaaaaaacg aagcataggg agtcaacggg aaaaatggtc
    21361 gcagtatttt gacaaaatcg ggcaaaaagg gctggttaca cgcttgcaca ttagttacat
    21421 ggttgcatta tgcttattag cgacaatcaa ttactattct tttttcgatc attcaatcgc
    21481 ttatacgatt acgctactga ttgctgggat ttttcactta tcttataaat atcagctgaa
    21541 taaaaaccac ttgaatcgga ctttccgcta aaaaatatcc gtacgataag tagagtgcct
    21601 tgaagatcgg caaaaaactg ccggactttt tagaggctgt gacaaagatt ttgtcacagc
    21661 ctctttttcc atataaacgg cgttcgccta ccagctgcct ggtaaatctg tttcctcttc
    21721 gttaagaagc gaaaatccgt caaatgtttc gccatcataa taacgaagca gctcatccat
    21781 ggacaagttc atcgtttttt ctttttcttc ttccgctgat tcagccagta gctgttcccg
    21841 atacacctga tacataccag ctattttttg ctgcatcgct tttctagttt caccttccgc
    21901 attgattatt ggcaattctt tgatatagcc acgataaatc aaccgatcat ctgaaaattc
    21961 aatgggtaat tcaacgagtg tcaacccgac ttcctgaaaa tattcttcca gcatctatta
    22021 tccccccatt ctttttctta aatgaatttt agcttaccta ctgaaaattt tccagtttca
    22081 ttattttgag aaaaaaaggg aatgtgacaa aaatcgtgtc acatcccctt tttccgaata
    22141 aatggtgttc ttctgccaac tcctcaatca taagtcacaa tattcaacaa acatgagaaa
    22201 ctgttttttg aaattgttcc ttgtgattca gagttaaacg acagtctcac aacctcttgg
    22261 taaacggcgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatatgag
    22321 aagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct
    22381 gatttcttat tcttcttcat ttgtagcaaa ataattacgg tgataaatga tacaacatac
    22441 actgataaga acaatggcac ctgcaaaata aaacgcatag ctttcaccaa tatgttcacc
    22501 aatcacacct gcaataaatg gtgctaaagc tccaccgaaa aagcggacaa aactatagct
    22561 ggacgaagca acgtttcgtt gcagacctgg tatttccata gcaatcgtcg tcaacagtgt
    22621 attgataatt ccttggaaaa atcctgctaa cacaatgctg atcggtacaa ggactggcat
    22681 agaagttcca gctcctaata acagcaaaca aataatgaat aagacaaatg ctgctaaaat
    22741 tgtccgataa gtcgtaaact tccgctcaag agccggtgca acgaaaatcg aagcaatagc
    22801 cagaagcaca ccccagccaa aaaatacaaa accgacttct aattcagaat agccaatcaa
    22861 taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa ttatataaca atgcgattag
    22921 tccaactgaa cgaagtttat gattgcttaa cgctttgaca ccttcccaaa ggcctacttt
    22981 tctctttggt ttctcagttt caggtaaaag taccgctagg gctataaaac cgcaaaacat
    23041 caaaaatgct accccaaaaa atggataacg ccatgaacca ctgcccaaga ccccaccgac
    23101 tagagggcca acagccattc cgcagccaag tgctgcttca tacatcatga ttgctttttc
    23161 tgtgttacca actaaaatac tgacgatcgt tgctaaagca gtagagacaa ataaagcatt
    23221 cccgacaccc cagccggcac gtaacccaat cagcattccg atattcccag ataatccgcc
    23281 aagaccagcg aatacaacga tgataaacaa tcctgtcatc aatgtttttt caaaccgatc
    23341 cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat gtaactagta
    23401 aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt ttttaagatt
    23461 gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac aactagcgct
    23521 gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg attctccaat
    23581 atttccatcc ctttctctct attagcatca tacatgattt attttacatg taaatatgca
    23641 catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga gaggtaggat
    23701 ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac cacgttctgg
    23761 gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc agatctatac
    23821 aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag acgagcactc
    23881 tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg aatggatcaa
    23941 agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct atgctttttc
    24001 gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac agctgaacaa
    24061 aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga aacaagaatt
    24121 tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac tccaatggtg
    24181 tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca gaacctttct
    24241 agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt tggaaaagaa
    24301 aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt ctagaactgc
    24361 attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca gtgttccatc
    24421 attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg atgatcaact
    24481 gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca gagaataatt
    24541 gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc accattccta
    24601 aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga tttactggta
    24661 gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa atgaaagcca
    24721 ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct tgatgcagcc
    24781 ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt gtaaaaatta
    24841 ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc acgaaaatca
    24901 tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc ttttatttca
    24961 taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa caaaatcgtt
    25021 cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc aataaaatta
    25081 gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc aatcgtcaga
    25141 cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc ctgagcaaac
    25201 cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg tctcatgaca
    25261 aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata tacttgaaag
    25321 cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc taataaggtt
    25381 gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga aaactgaaaa
    25441 tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg tcccatgatg
    25501 ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg tttcatctgc
    25561 tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt cgcattcttt
    25621 tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc cggtctgatt
    25681 tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt tttcttttct
    25741 tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc tcgtagctga
    25801 gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga aaactctcgg
    25861 atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa atagcgatca
    25921 tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag tcgatcaata
    25981 ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc ataagaagaa
    26041 agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg ctctgaagtt
    26101 tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt tgcttcagcg
    26161 agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt cccatgatcg
    26221 ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc attttctcct
    26281 atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag ttctagctgg
    26341 ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat atgttttaca
    26401 ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa aatctgtttg
    26461 caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac taaatcacca
    26521 aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat actccatcca
    26581 tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa aagttcgaaa
    26641 cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt accacacacg
    26701 attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg aacgtagtga
    26761 tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt cctattgata
    26821 aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc aactgatctg
    26881 cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact aacatgaatt
    26941 gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct cttgaaaaac
    27001 tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt ggcatcttcc
    27061 ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc atatcgccaa
    27121 aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg atcaaaggac
    27181 gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt tgcatattga
    27241 cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg tgtgggattt
    27301 tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt gaaaaatcat
    27361 ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca aagttgggta
    27421 ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa agccggggat
    27481 agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt ctgggctttt
    27541 tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg cgaaacatag
    27601 ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat ctgcaacttt
    27661 tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat gatctcgtat
    27721 ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg atcggctaat
    27781 ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc ctttctaata
    27841 attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa agtcaaagaa
    27901 tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag aaattttttg
    27961 tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat tattgattct
    28021 tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc aaaaatttcc
    28081 gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta tgcttaaaca
    28141 gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac ggtgttcaaa
    28201 aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct gtttttgttt
    28261 ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta ataaccataa
    28321 aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc cttagcactc
    28381 aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata aattggcttg
    28441 gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt taaagagttg
    28501 atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta taaacggtag
    28561 acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca gcctacagtt
    28621 ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt atgacggtta
    28681 tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt cagattatag
    28741 gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata acgacctgtt
    28801 agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc ggaatagtag
    28861 taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg caatgattct
    28921 ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc attcacatat
    28981 tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc aaatagattg
    29041 taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat ggcttgagca
    29101 atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt tgaggcaata
    29161 ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc atagccagta
    29221 tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg cccatctgtc
    29281 aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc tgatgtactg
    29341 tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga aattgtattt
    29401 tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc tgtttctgaa
    29461 gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac tgttgtgttg
    29521 gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc tttcatcttt
    29581 tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa agtaaaagca
    29641 agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct ttttctacat
    29701 aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat ggtcgtttga
    29761 ataactattt aacaagtaat tctctttgga tattatccgt tatggattat acttcctgtg
    29821 tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct atcgcaaatg
    29881 ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct tcatgaagtt
    29941 caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca gctcctggag
    30001 agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct tccatgattg
    30061 cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca acatgtcccg
    30121 ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt ggaagtacgg
    30181 cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc cattcatcac
    30241 ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac aatacatcga
    30301 ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca aaatctctta
    30361 catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt gcttttaggc
    30421 gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat agcttcgttg
    30481 ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct gataaatcat
    30541 tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat ctcttatttc
    30601 atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg attcctgcaa
    30661 cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca atggcattct
    30721 cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca tttttcttga
    30781 tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga tggttcaccg
    30841 tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg ccacctaagt
    30901 caacgaacat cacttgacga ttgataaaga catttttccc tatactaata tgtttgccga
    30961 aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca ctaatgatat
    31021 tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc gctgctaaag
    31081 gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc gttactcgaa
    31141 tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct ccttttatcc
    31201 ttactttata acggacggct tatcatgtct aatacttata aaacatactt aactattcct
    31261 tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg gatattcgta
    31321 tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca gcaaaatctc
    31381 tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa gaacttggtg
    31441 tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt cgatatcttt
    31501 attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat ttaacaaatg
    31561 acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca atctctttca
    31621 tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt catttatata
    31681 gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat tttggactcg
    31741 taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc caagatcgtt
    31801 ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc catccgcagg
    31861 aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt caactagctg
    31921 aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac ttattgtaca
    31981 atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat gggatcatca
    32041 atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa gcttcactga
    32101 atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt ttagatgtcc
    32161 taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt ttaacaacga
    32221 ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt atttcttcct
    32281 tttatttctg ataattccag taaattacta taattataaa aaaagaattg acaaaacata
    32341 actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact agatcatact
    32401 aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg tctatttttg
    32461 tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa agggtgcgcg
    32521 catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg gtggacagga
    32581 aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt tgatttttga
    32641 tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt tgcaaaattt
    32701 acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg atcatgcgga
    32761 aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg accacattgc
    32821 tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt taagagaaat
    32881 cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt atctgacaaa
    32941 agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc atattcacag
    33001 cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga ttgtatctga
    33061 catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac cattttcact
    33121 gaattattca agactggtga atcatttacg actgcttcta caacgaaccc atgcgcaaca
    33181 atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc cagaaagcta
    33241 taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg cgatcacgaa
    33301 agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa tcgtacaaac
    33361 aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg aatgggacgt
    33421 tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg gatcggaaac
    33481 tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg cggtaacgca
    33541 gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat gtcagtggac
    33601 aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt aaatgtttta
    33661 aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga tccatctttt
    33721 gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc agctctttat
    33781 aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg caagcgtcta
    33841 gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact gctcgtatgg
    33901 cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt aggctggggt
    33961 ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt gcatcaagca
    34021 atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa atttttggct
    34081 tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc aatcatgatg
    34141 ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga aaacaaagca
    34201 aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg tgtgacagaa
    34261 ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca tgcaattttc
    34321 actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt tgcctttagt
    34381 gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca gccattgatg
    34441 ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa ctttgctatc
    34501 aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga tgacgatact
    34561 gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta cgctgcatta
    34621 ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg tttgcaagtc
    34681 aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc tggtatgaag
    34741 atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt cgttgcagat
    34801 gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa caccgtggtt
    34861 aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat cgcaaatggg
    34921 aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat gatgggtgac
    34981 ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg tacgatcaca
    35041 aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat cgaagtcttg
    35101 cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt atatgtagaa
    35161 gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc cgctattcaa
    35221 tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa agtccaaagc
    35281 ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc tgtttccaac
    35341 aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag gagctgagac
    35401 aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc caactccgcg
    35461 gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac tcttcttatt
    35521 acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact aatcggcttt
    35581 ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct gcttcaagag
    35641 aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct aactgatcct
    35701 ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg aatccggctt
    35761 cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac tctttacctg
    35821 tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg gataattcta
    35881 agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca tattcacgtt
    35941 tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca gtttttaaag
    36001 cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa aatgtatggg
    36061 aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct aatacactga
    36121 tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca gctgcttgtg
    36181 ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca gagacaaaaa
    36241 gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca taatcaccaa
    36301 tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta cgtactagta
    36361 catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa ccaccaaaac
    36421 ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct ttttatttgt
    36481 taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg tggtaagata
    36541 agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca atttagtgat
    36601 gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact ttctagtgag
    36661 atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat gagtcagctc
    36721 aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct tgccaaaagt
    36781 ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac acagcttatc
    36841 caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca agaagcttta
    36901 gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa gatcacactt
    36961 gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga aaacacaggc
    37021 ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga tcggaatcgt
    37081 ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct ttttcctctc
    37141 taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt tattgactac
    37201 cggtatccag atttctccca tatatttccc gttggtaact cccatttcga ctacagcatt
    37261 tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa aggccgtatt
    37321 cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgcttacaa caagatacgt
    37381 tccttttggg aatgggatga ttcgcgtagt gtttggaaca tcctgactcg tttgaacacc
    37441 gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat aatgattttc
    37501 agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct caaatgcttg
    37561 tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca caccaatacc
    37621 cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc ccaccctttc
    37681 tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta cgctcttttt
    37741 tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc aaatctcact
    37801 gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag cttcgactaa
    37861 cccataatct gaagaaggaa gccattctgc atagattttc ccccaagtat cttgtaaagt
    37921 ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta tagcaagcat
    37981 ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt gcatcaaact
    38041 gcccttttct tccaagcggt ctccatcaaa tccataagaa acgttgatta cttgatttgg
    38101 atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg tctgcgctaa
    38161 cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg ccacacctgc
    38221 cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc ctctgacatc
    38281 aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctactga tttctgaagg
    38341 cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt catatccata
    38401 tctaaacgcc gtttctgtca ctcttgatcc atctagaaga tcgcgattag ccaaagccaa
    38461 tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa agatgcgttt
    38521 taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa gtccttctgt
    38581 caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat ttatcttcct
    38641 ccttgattgc ctttttatca tattatcaaa gaaagtcaat tacttgatga atcaggaacc
    38701 aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa gcagcgtttc
    38761 gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa ataaattttg
    38821 gggaagacag attctaagta aacaaagcgg taaaaatatc ttttattccc ttagttaatc
    38881 cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag aacgctgctt
    38941 ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta tttatttcta
    39001 acaaaatcac ttcatttttg tcagataaat aaaaaacaaa aaaagttcca tctttaaaac
    39061 acgctattct tttacttttt ctcacattct ctaagaaaag aaaaggagaa tgaatttttg
    39121 acaattcgag caaaagtgat tatcaaatat gtaaaatcag ctactttttt cttaaacgtt
    39181 tctttatact aaatataaat aagtgtttca tgaaaggaga actagatgaa taattggctt
    39241 gagtattttc ctgaaaacgt tttggaacgc ggttacagct atcatctgca cggttttgtc
    39301 cgtcatttga actatacaag caagtacttg tccgcaacag tttctggcac ggaagattat
    39361 aaggtagtga tcacttggga tgaaaagacc aacatgactt gtgattgtct atatgccata
    39421 gaaggaaaaa aatgcaaaca catggctgct gtcttattcg cttacgaaga acgtccaata
    39481 aaaaaatcca actactcact ttccgaactc tcgtcccttg tttcctccgc tagttcctct
    39541 cttgtacggg agcttttaac agagattctt atagaacatc cgcactttat cgaacgcttc
    39601 aaagttaaga tgccatttca tgcaataaat tattcagata aattaacaac tatcatccat
    39661 aaatacgatc atattatcaa gaaaaacaaa aacaggaaaa cagcgaagtt catcatggag
    39721 atgcgaaagt tcatccaaga agcagttgaa tcgcttattc agcaaaatgc ctatcttccc
    39781 gcattcgaat tgatcaatga agtgattgct actctcgaaa cattctattg ggagcctgag
    39841 gatgaacgaa cccttcttct gatagaggac tgttattatt tatggaaaga attattagca
    39901 gaagcacctc atgcagaaaa aaggcaaatg ttctcttggt tcgtctgcca agtcgatcat
    39961 actgacgcct cttattcaaa acgctactct ataaaaatcc tgaaagaaga ctttcgggaa
    40021 aaagaatttt ctaaccaaaa gaaaaaaata gataaaaaac taaagaaacg gtaaagaaag
    40081 acgatttcaa tgaaaaatag caagtaaacg ataacaacca tcctaaatcc acaagaaata
    40141 cagcagacct tgctttaaaa acacgaatca tgcaaaggat gaagtaaaga agacttaacg
    40201 cgtacccaat attagtcatt tgactattag gtacgcgttt tgttttataa gtcatgacaa
    40261 atttccttcg ttgctggata gattttccgg cattgttgat agattttatg atacttttct
    40321 acattttgta agagaggttc cgctgcatct gtatattgga taaaattctg acaacaactt
    40381 tcaggtgtgt cgaaccaccc taaccctagt gcagcaatca ttgttgcgcc taatgcaggt
    40441 ccttcctcca tacttaaaca atagaccgtt gcatcgaaaa tatcagcttg gatttgtagc
    40501 catgcaagat ttttcgcgcc tcctcccacc gaaacaatac gtgtaaatct gcgacccgct
    40561 agttctttac gtttcaatcc caccattgac aaatgctcat taaaaattta gaatattcac
    40621 aagtttaacg acaattatga aaatttatgt cgtgctaaag gagttttgtt attggatgaa
    40681 aagttagata taaaaactgc gatcaaagac acgttaccta ccgttttcgg ttatatcggt
    40741 attggacttg catttggtat cgttgggaaa gctgccggat ttcatccact agtcgtcacg
    40801 ttgatgtccc tgctggtcta tgctggttct gcccaattta tcacagtcag catgcttgct
    40861 agtcacagcc cattgctttc catcgttttc tcgacctttc tagtcaattc ccgaatgatt
    40921 ctgatgagta tgacgattgc tccttatttc aagaaaaatc ggctgcttca aaatctgctg
    40981 attggcacat tgctgacaga tgaaagtttc gcactgggaa tgaataaatt gaactatact
    41041 ggacaaaagt taaactttcg ttggatgaat acagctaatt ggatttctta tttgacgtgg
    41101 gtgggttctt ctcttgttgg cgcactttta gggaatttta ttactgatcc gaaaaagttt
    41161 ggtttggatt tcgccatcgt cgctatgttt atcgggttac tttatcttca agtcatttct
    41221 gatagaaaca cctctaaacg attacagttg atcctgattg gtctcacttt gattctggta
    41281 tatgtcggac tgatttttat cccaagcaac ttagtgattg tagttgtaac actgattggg
    41341 tgcggattgg gggtgtggat caaacatgcc ttcttttgag tttacattgc tgactatttt
    41401 aggttgtact ttagcaactt ggctttctag ggtcctacca tttgtcttac tgaaaaaatt
    41461 cgatttaccg caaccgttgc ttgagtattt aagttttgtc ccgatcgtca ttatgtctgc
    41521 tttatggttc agcagtctat ttacccaaaa tatcggacat ctgccgcaga tcaatctcga
    41581 aaatgctttg gcttcagttc ctacactttt agctgctatt ctttcaaaaa gcttattggt
    41641 gatcgtcttg gcaggaattc tttccctttc gttgattcgc ttattataaa taaaaaaaca
    41701 agagcttatg gcaaattact gtcatgcgct cttgttttta tcaaagtcaa ataagaaaaa
    41761 aatcggcgta ttcttgctta tccgttgaac ggaccactaa caacgacttt tcctaacata
    41821 tgtcctgttt ctacgtcttt ggttgcttgt ttcaagttct tcgcattcat cccatctgaa
    41881 tagaccttgc tcaatgtact gcggataatc ccttcatttg ctaaagcagc aatgtgcgct
    41941 aaagcttctc cttgcgtttc gatttcatag ttatagtctg ttttagcgaa catatactcc
    42001 cagtcgaagc tgatagattg gtttttccaa acacctatat ctaacggctc tttgatccct
    42061 acgatcgttc ccacatgtcc aaatggtcgg acaaggtgct tgatttgatc aaagtagcgg
    42121 gtaatatcga ataatacagc gacatactct acttgatcga ttcctaattc acgaaaactt
    42181 ttacttaagt cgtgatgata atcgattgga tagtctacac ctgttttttt cagccattca
    42241 aaattcttcg gacttgcagt cgcgtagact tctaggccag cccaatgcgc tagttgattc
    42301 aaaatcgaac cgacgccccc ggatccgttg atcactaaga tttttttccc gcgattggca
    42361 ttttcttctg gagttagacc aaatttttca aataacaatt cataagcggt caatgaggtc
    42421 aacggcaagg ctgcagcttc ttcatctgaa agattttttg gtgcaagagc gacaatacgt
    42481 tcgtcaacta gttggtattc ttgattgctt cctgctcttg ttgttgtacc tgcgtaaaaa
    42541 acgcgatcgc cgacagaaaa tttttgcact tcgtcaccta cagcaacaac tttcccgact
    42601 ccatcaaaac ctaagatacg taaagcattt cttatcccgt tatcttggcg aagtttcgta
    42661 tcgacaggat taacagatac tgcgttgact tttaccagaa gatcccgtga actgggtaca
    42721 ggtatctgtt ctttttcatc aataaagctt tttggatcat cgatcggcaa cccttcataa
    42781 aaaccgacta ccttcattaa ttccttttct gacatacgaa ttcctcctta gcttcatcaa
    42841 gttggagcat ccatacagtg ttcatttcaa tatatatagt atgctatttg ttccaccttt
    42901 tttattcatc ataattattc tcttacttaa agtaaactat ttgcactttt ttggttaaaa
    42961 aagaggttgc gacaagactt ttgtcacaac ctcttataaa cggtgttctc ctaccaactc
    43021 ttcggtcaca agtcaaaata ttcatcaaac atgagaagct gttttttgaa attgctcctt
    43081 gtgactcaga gttaaacggc aggctcacaa cctctgatat aaacggtgtt caatcaactg
    43141 accctcggta ttagctcaaa aaacgacaaa atatgaagag ctatttttgt ttggctgttc
    43201 ttattacttg atacaggaca gctttttcac gacctctaat tcacggtgtt caagcaagaa
    43261 atcattgttc actgaaaagc gttggatgtt ctttttcaaa ggtctctaag atctctcgta
    43321 cttcatcagt agatttcgta ttcatcaact gaactcgcaa gtcatttgct cctggaaatc
    43381 ctttgatata gattttaaag aaacgatgta gtcctgtgat ggaacgcggg atgatttctg
    43441 aataatgatc ttgcagatct agttgcagac gaagtagtcc gatcagttct ttttctgtat
    43501 gttccctcgg gtctttttca aatgcataag gattcttgaa aatccctcgg ccaatcatga
    43561 tgccatcaac gccatatttc tcggccaatt cttctcctgt ctggcgatcg gggatatctc
    43621 cattgatcgt gattagtgtt tgtggagcga tttggtcacg taaagcaatg attttaggaa
    43681 tgacttccca atgtgcatct actttactca tctctttacg cgtacgcaaa tggacagaaa
    43741 gattggctat atcttgatga agtaggtgac tgatccattc ttccatctca gacatttccg
    43801 tatagccgat acgcgtcttg acgctgacag gcaaaccgcc agctttagct gcttcaatca
    43861 attgtgccgc tacttctgga cgaaggatca agccactgcc ttttccacgt tctgctacat
    43921 tagggactgg gcagcccatg ttgatatcga ttccttggaa gcccatttct gccatagcaa
    43981 tgctcatttc tctaaaaaat tctggcttgt ctccccaaat atgggcaacc atcggctgtt
    44041 catcttctgt aaatgttaat cttcctcgta cactgtcttt tccatctgga tgacagaaac
    44101 tgtctgagtt tgtaaactca gtaaagaaga catccggtgc tgctgcatgc tttacgacat
    44161 ggcgaaatac aacatctgtt acatcttcca ttggtgctaa aacaaaaaaa ggctttggca
    44221 gctctttcca aaaattagta gtcatttatt tgttactctc cattctatca agcagtgaag
    44281 tcgcaatctt tctcaaaaac attgtcatta tactaggaaa caaaaaaaga agcaaagtga
    44341 acagcttaaa agatgtttct gattgagcaa aaaaaagact gtgacaaaaa tcttatcaca
    44401 gtccattttc cggaataaat ggtgttcaaa agcttgcatt tgcggtaata agtccaacca
    44461 aacgaaaaat aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc
    44521 tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta ttcgctcaaa
    44581 aaacaacaaa atatgaggaa ctattttccc gtttgtttct ctaatactca ggtcaaaacg
    44641 ctgatttcac aacctctttt ctaacttctc aatcctcttc ctttattgat caaataccaa
    44701 gtaacggcgt acaaaacaac gatgaataac acaagtacga tcatcgatgt tgctactggc
    44761 acatcgatcg ttccaaggaa tccgtaacgg aaaccggaga tcatataaac gatcggattg
    44821 acttttgaaa cagcttgcca aaagggcggt aacatagaga tggcatagaa aacgccacct
    44881 agataagtta acggctgcaa gacaaatgta ggcacgatcg atacatcatc ataagactga
    44941 gcaaaaatcc cgttgagcag tcctgctaat gaaaacaaga ttgccgtcat caataatgta
    45001 atgatgacca tgaaccatga atacacatga agtggtacaa agaacaagga aatgatcgtt
    45061 acgagagagc cgacgagaat actgcgaccc acaccaccaa tcacaaagcc ccaaataatc
    45121 acatgcgtag gaacaggtgc taccaagatt tcttcgatat ttttctgaaa tttttgagag
    45181 aagaaggacg aagaaacatt ggcatacgaa ctagtgatag cagacatcat aatcaaacca
    45241 ggaacgatga attccatata tgaaaaacca cccatgtcac cgatccgccc accgatcatt
    45301 tttccgaaaa taacaaaata caaggatgtc gtgatcactg gcggaaccag tgtttggacc
    45361 catatacgca aatagcggtt cgtttccttg atagctaaac tttttaatgc agtaaaataa
    45421 agattaaaca tttttctcct ccgtatgatt attttcttct gtgattttca agaacagttc
    45481 ttctaaacga ttcgacttat tcctcataga aagaactttg attccttgtc ttgtaagctg
    45541 atcgaagatt ccgttcaccc cttgatttcg ttcaacttcg acagcgattg tctgctcatc
    45601 ttcaaatgta caagcatatc cttcaatcac aggtttggta tcatatggcg ctaaatcaaa
    45661 gataaatgtt tcaaattgca gttttgacag aagctctttc atacttgtat tctcgatcag
    45721 ttcacccgat tggatgattc cgatgttgcg acaaagcatt tcagcttctt ctagataatg
    45781 cgtcgtcaaa atgatcgttg taccattttc gttcaattct cgtagaaact cccacatctc
    45841 gcgtctcaac tcgatgtcaa ctcctgctgt tggttcatcc aagatcagca aacgtggttc
    45901 atgcatcaat gccctagcaa tcatcagtct tcgcttcatt cccccggaaa gcatacgtgc
    45961 acggacattt ctcttttccc aaagattgga ctgcttcaaa tatttttcgc tgcgtttcat
    46021 cgcttctttg cgagagacac cgtagtagcc tgcttgattc acgacgattt gctggaccgt
    46081 ttcaaaaggg ttaaaattga actcttgtgg aaccaatcca atctgttgct ttgctcgaac
    46141 tagatccttg tccagatcat aaccgaagac attcactttt ccagatgttt tattgactaa
    46201 agaagtaata atcccgatcg ttgtagactt cccagctcca tttggcccta atagcgcata
    46261 gaaatctcct tctttcaccg aaagatcgat tccccgcaat gcttcgaccc cacttccata
    46321 gatctttttc aaattattta tttcaagcgc atttgccatt tatttcctat ctccttatag
    46381 acatgattct taattgtatc atggaataca gcagatgaaa aaacaacttg gcttccacac
    46441 aaaaacacac ctgctaacaa agaggagatg tgttttctag ttcatgatca tcttcgatag
    46501 aataactatc taaacaactc tggaactttt aaaatcataa aagtaccgac agcaactact
    46561 acgatgacat atagaagatc cttataactg aggttcttgt cttttttatt catgttaagc
    46621 tttcctccgt ccttttatcg aaagcttttt tcttttattt taaccgaaat aaataaaatg
    46681 gaaatagtta ttttcaattt ctgttttcgc tgtttgtttt acggagaact ttgccatgga
    46741 acgtgcgtct cgtcgctgat gctaagtttt ttcgataaaa gctgctagaa ggaagacagc
    46801 gtggtttatt ttgtatcgtc ttttgctaaa tcttccaaga tcatctggac agcagactcg
    46861 ttcccattga attctgccac ttttttggca tcaatgtact tcttttgata atcgtctttt
    46921 tggacccacg catcatgctt cgtttgataa agttaatcaa acggatacca ctgacgctgt
    46981 tcatcgaaca acaaactttc tttttgggcc tgttcaacta tggatttagg ataattcaaa
    47041 actttcagca aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa tttgaaatca
    47101 aaactgattc cttttccttc tgttacttgt tcttcaaagt ggacattctc acaataattc
    47161 tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga tggatactcc
    47221 gcaagccacc gaacgactga agcggaagaa gcaatccgtt cgatcgtatt tgttcctttt
    47281 aaaatctcat ctacgaagca gtagcagcgt tctttggaag ccacttggtc cagcaaacgt
    47341 ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt caaataaatc atcttcgatt
    47401 gccatggaag tcaacacatg tccaggttgc agggtaaact gttctgcaat cgctgtttgg
    47461 atcgtctgtg ctagaataca gctgatggct atacttttga catacgtaga ttttcctgat
    47521 gcattggaac ccgtgactaa tgtattcttc tgccagttga ctggattaac taccgcttct
    47581 tttaacaatg gatgatagat atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg
    47641 caagtgatcg gcatataggt gcgataattc aaaacggctg ctgcaacttc caattctccc
    47701 agcaattccc acagcttgat ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa
    47761 tgataggaga taaatggcag catgaacatg atgttgacat attcaaaaat caattctgct
    47821 tcactcccgc tcttcattcg aaatgaaaaa cctaatttag ggatgtctct taatggttta
    47881 gtttgctgtt tcagctcttt ttgaagtggt gtcgtcattt ttgctaactg attggcggct
    47941 gaaatcgttg aaaccaaata actcatgcta ttcagctctg tttctagtgt ttgtttcttc
    48001 actagataat agaccgtatt gaatactaag ccaccgattg ctagatagat tcctagtact
    48061 tgccctaaaa gaagcaaacc tacacccacc agcggaagca agccaagaaa aataaataaa
    48121 cctacgcgtc caatcttttt actttttcca tccgctagat attgttttgc aaaattatga
    48181 tctttttttc ctaaacgagc gaattgatat tggattcttt ctctcagttg aggattttcc
    48241 tgataaaaag caattagttt ttctaactga taatcttctc caaaatcaaa gctacgtaaa
    48301 cgctgataca acgcttctga acctacacta gaataggtac tgttgatccc ttcaaatact
    48361 tcgaacatat ccaagtcata ccaagtaaga tcgtcgattt cactatccca tgaacggaat
    48421 tttttctccg tgagccacgc atccttcaaa ctttcttctt tatcaaatct cggttgataa
    48481 ggaatttttc cccaattact acgaactgtt tcctttactt tcaaacgtga aaaaatgtct
    48541 aaaacaacta ataaaataac aactgcaata ataattaaaa cgataaattc tctttggccc
    48601 atatccctct ccgttccttg atttgttagt tttagtatag cactgttgcg ctatttgaaa
    48661 agggggtaca aaaataactg tcttcgttac ctaattcgta tcaatcacac caaaataaaa
    48721 acaccttcca aaaaagaaga tgtctttaaa acgagataca attttttgtc attcacttca
    48781 ggtaaattag agccaattaa accctccgca acttgacgga gatggagaag atgcccccgt
    48841 ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac aggtaaagcc
    48901 cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa cgctggaaga
    48961 ctttttcaac agaggtttgc gctcctggtt tgataggatt ggccactgtt aatcgaatga
    49021 atgaatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc aataaattct
    49081 gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca gttgtattta
    49141 tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca taaaaagaga
    49201 aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt tctttttgct
    49261 tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat cttgcaacag
    49321 attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc tgttccagta
    49381 attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa aggccgacga
    49441 tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt tcattttcat
    49501 ccacccatgt attcagtgca tctactaggt ccattagatt cttttctcga aattcgactg
    49561 aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta gttaatctct
    49621 tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg agaagcaaca
    49681 gccacttaat caaatctaaa ccccactccc cacaccgtat gaatatacac agcatgtgcc
    49741 tttgctagtt tccctcgaag attggaaata tgcatattca ctgttttctc actatcaaaa
    49801 tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt tttcggatgt
    49861 tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc cacaccattc
    49921 acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat gcgttgcgac
    49981 agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg taacaacagt
    50041 tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa caaggaaact
    50101 ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga tgtctgtcgg
    50161 atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag aaggatcaaa
    50221 tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc atcaagaagt
    50281 ttgtatctct gttcaagaac ctcctccagt aaagaatgaa cagcttcatc gtcttcgata
    50341 atcaaaatag tttccagcag gaacacctac ttaatgtcag atttttggaa aataaacatt
    50401 cctagagctg atgataccac gattgttact agactgacca gaatcatttt cggtgcttgc
    50461 gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt aaaccactcc
    50521 gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaacgaaggc cgttcccatg
    50581 atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa tgtcaaagcc
    50641 actggaacag ataatgtcgc taatatcagc agaagcaata caggaaatac ttttagaaca
    50701 taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag tgtgtaagca
    50761 atcatggcta gccccgtaat caaaaccacg ccaagtgttg tgatcaaaag cgtaacgaga
    50821 agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt gcgaatcgta
    50881 ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc cagaagaatc
    50941 catgcagcca ttttttccac cattaaaatc cctgttgcac cgttgatgac tgtatccgtt
    51001 gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca agaataaaaa
    51061 agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt ccatactttt
    51121 attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt gtaagaaata
    51181 atcttccagt ttttgcccaa ctaggtggat accatccaca ggaacttttg cctctaccaa
    51241 tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa cacggctaga
    51301 ttggacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt gttccgtcgc
    51361 cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt tcgataattc
    51421 tttgatcaag tgtccttcat gcaaaatacc gtagtgagtg gcgatctgtg acaattcatc
    51481 tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac gttggatgat
    51541 ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat ccaaaatcaa
    51601 aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca tacctaaaga
    51661 gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca gcaggtcttc
    51721 gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat ctcttgcagt
    51781 taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc ttgctgcctg
    51841 caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca aacccataat
    51901 agttctcata aaggtagact tacccgctcc attctcccca atcaaaccat agatcattcc
    51961 acgtttgatt tcgatcgata ctttatctaa tgctttggca gcaccgtatt ttttggttat
    52021 accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc tttcttattg
    52081 ataagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata aagaaagtgt
    52141 aaattttcca attgaatttc aagaaaaatc agactgtctc gcagacgtgg ttgagttgtc
    52201 tgccaatcaa agagcgcacc aaacagtgct aatccaagtg gactggccac catggtaatc
    52261 gttgttatcg ttgacatcag acgtccttgt atatgcgttg gaacttctcg ctgaagccag
    52321 accatataaa aagcaaaaga aaacattaat ccttaaaaac ggacttatga aaaataatac
    52381 atgccgtttg ggatttacaa ttagctacca aataagaggt tatgacaaaa gacttttcac
    52441 aatcccttct tccgaataaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata
    52501 tttatcaaac atgagaagca atttttgtaa attccttctt atttcttgaa gctgaccact
    52561 tctgtcacaa cctcagaagc ggataaataa tattgtttga tgatacttta tctttttccc
    52621 gtttgtttat agatcgaatg atattataga ccaagaatcc tacctattaa catagctaaa
    52681 aacacaaaaa aggcactttc caaattgtgg aaagtgccag aaaagttagg attaaggtaa
    52741 ctgcgctcag cattgcttcg cccagtacga ttctcgtaac tcgtgaactc gaacgataat
    52801 cgcaattaac gttttgagaa ctgtgaagct ttacgggctt tcttaagacc tggttaaaca
    52861 aaactctatt ttattgcatt ttacaggaat gtatttcatg cgaaattaat aagtctttgt
    52921 ttatagagtt ttactagggt ataaaaagtt gtatgttatc attacctcaa aacattacct
    52981 cagcttggcg agttggttga ttgaagaata aatcatttat ctagatatct caataaaaaa
    53041 tgatttctaa gaaacttgta ttttcgtgtt attatttttt tgccctatca aaatttctaa
    53101 ttcttctagc gtaatatgat tatgacacat aatcgattca attacttcaa ta
//
LOCUS       DAZKCZ010000008        49929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000008 DAZKCZ010000000
VERSION     DAZKCZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.008"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..787
                     /locus_tag="ACG8KN_000395"
     CDS             107..787
                     /locus_tag="ACG8KN_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9187482.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            877..1911
                     /locus_tag="ACG8KN_000396"
     CDS             877..1911
                     /locus_tag="ACG8KN_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9187483.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1925..2260
                     /locus_tag="ACG8KN_000397"
     CDS             1925..2260
                     /locus_tag="ACG8KN_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187484.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2244..4724
                     /locus_tag="ACG8KN_000398"
     CDS             2244..4724
                     /locus_tag="ACG8KN_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187485.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4780..6339
                     /locus_tag="ACG8KN_000399"
     CDS             4780..6339
                     /locus_tag="ACG8KN_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9187486.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6336..7700
                     /locus_tag="ACG8KN_000400"
     CDS             6336..7700
                     /locus_tag="ACG8KN_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9187487.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7871..8941
                     /locus_tag="ACG8KN_000401"
     CDS             7871..8941
                     /locus_tag="ACG8KN_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9187488.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            8956..9624
                     /locus_tag="ACG8KN_000402"
     CDS             8956..9624
                     /locus_tag="ACG8KN_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187489.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9644..10222
                     /locus_tag="ACG8KN_000403"
     CDS             9644..10222
                     /locus_tag="ACG8KN_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9187490.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10625..11536
                     /locus_tag="ACG8KN_000404"
     CDS             10625..11536
                     /locus_tag="ACG8KN_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9187491.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11792..12709)
                     /gene="rnhC"
                     /locus_tag="ACG8KN_000405"
     CDS             complement(11792..12709)
                     /gene="rnhC"
                     /locus_tag="ACG8KN_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFP9187492.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            12911..13348
                     /gene="zapA"
                     /locus_tag="ACG8KN_000406"
     CDS             12911..13348
                     /gene="zapA"
                     /locus_tag="ACG8KN_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9187493.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13406..13954
                     /locus_tag="ACG8KN_000407"
     CDS             13406..13954
                     /locus_tag="ACG8KN_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFP9187494.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14069..16429
                     /locus_tag="ACG8KN_000408"
     CDS             14069..16429
                     /locus_tag="ACG8KN_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFP9187495.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16516..16830
                     /gene="trxA"
                     /locus_tag="ACG8KN_000409"
     CDS             16516..16830
                     /gene="trxA"
                     /locus_tag="ACG8KN_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFP9187496.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17012..18790
                     /gene="uvrC"
                     /locus_tag="ACG8KN_000410"
     CDS             17012..18790
                     /gene="uvrC"
                     /locus_tag="ACG8KN_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9187497.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            18935..19834
                     /locus_tag="ACG8KN_000411"
     CDS             18935..19834
                     /locus_tag="ACG8KN_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187498.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19827..21089
                     /locus_tag="ACG8KN_000412"
     CDS             19827..21089
                     /locus_tag="ACG8KN_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9187499.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21250..23148
                     /locus_tag="ACG8KN_000413"
     CDS             21250..23148
                     /locus_tag="ACG8KN_000413"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFP9187500.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(23447..24166)
                     /locus_tag="ACG8KN_000414"
     CDS             complement(23447..24166)
                     /locus_tag="ACG8KN_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFP9187501.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    24356..24403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24503..24835
                     /locus_tag="ACG8KN_000415"
     CDS             24503..24835
                     /locus_tag="ACG8KN_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187502.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            24822..26825
                     /locus_tag="ACG8KN_000416"
     CDS             24822..26825
                     /locus_tag="ACG8KN_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFP9187503.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            26844..27314
                     /locus_tag="ACG8KN_000417"
     CDS             26844..27314
                     /locus_tag="ACG8KN_000417"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFP9187504.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27335..27919
                     /locus_tag="ACG8KN_000418"
     CDS             27335..27919
                     /locus_tag="ACG8KN_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFP9187505.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            27934..28920
                     /locus_tag="ACG8KN_000419"
     CDS             27934..28920
                     /locus_tag="ACG8KN_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFP9187506.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            28910..29221
                     /locus_tag="ACG8KN_000420"
     CDS             28910..29221
                     /locus_tag="ACG8KN_000420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFP9187507.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29243..31024
                     /locus_tag="ACG8KN_000421"
     CDS             29243..31024
                     /locus_tag="ACG8KN_000421"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFP9187508.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31017..32393
                     /locus_tag="ACG8KN_000422"
     CDS             31017..32393
                     /locus_tag="ACG8KN_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFP9187509.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32400..33035
                     /locus_tag="ACG8KN_000423"
     CDS             32400..33035
                     /locus_tag="ACG8KN_000423"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFP9187510.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33065..34420
                     /locus_tag="ACG8KN_000424"
     CDS             33065..34420
                     /locus_tag="ACG8KN_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9187511.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34612..36006)
                     /locus_tag="ACG8KN_000425"
     CDS             complement(34612..36006)
                     /locus_tag="ACG8KN_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9187512.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36098..37069
                     /locus_tag="ACG8KN_000426"
     CDS             36098..37069
                     /locus_tag="ACG8KN_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFP9187513.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37066..37847
                     /locus_tag="ACG8KN_000427"
                     /pseudo
     CDS             37066..37847
                     /locus_tag="ACG8KN_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(38016..38387)
                     /locus_tag="ACG8KN_000428"
     CDS             complement(38016..38387)
                     /locus_tag="ACG8KN_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9187514.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            38521..38964
                     /locus_tag="ACG8KN_000429"
     CDS             38521..38964
                     /locus_tag="ACG8KN_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9187515.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39007..39816)
                     /gene="yidA"
                     /locus_tag="ACG8KN_000430"
     CDS             complement(39007..39816)
                     /gene="yidA"
                     /locus_tag="ACG8KN_000430"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9187516.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(39835..41202)
                     /locus_tag="ACG8KN_000431"
     CDS             complement(39835..41202)
                     /locus_tag="ACG8KN_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9187517.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41186..42016)
                     /locus_tag="ACG8KN_000432"
     CDS             complement(41186..42016)
                     /locus_tag="ACG8KN_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFP9187518.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42179..42616
                     /locus_tag="ACG8KN_000433"
     CDS             42179..42616
                     /locus_tag="ACG8KN_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFP9187519.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42692..43312
                     /gene="rpoE"
                     /locus_tag="ACG8KN_000434"
     CDS             42692..43312
                     /gene="rpoE"
                     /locus_tag="ACG8KN_000434"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFP9187520.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43501..44007
                     /locus_tag="ACG8KN_000435"
     CDS             43501..44007
                     /locus_tag="ACG8KN_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP9187521.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44024..44923
                     /locus_tag="ACG8KN_000436"
     CDS             44024..44923
                     /locus_tag="ACG8KN_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFP9187522.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45010..45895
                     /locus_tag="ACG8KN_000437"
                     /pseudo
     CDS             45010..45895
                     /locus_tag="ACG8KN_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(46087..46158)
                     /locus_tag="ACG8KN_000438"
     tRNA            complement(46087..46158)
                     /locus_tag="ACG8KN_000438"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46123..46125),aa:Arg,seq:ccg)
     gene            46218..47552
                     /gene="rpoN"
                     /locus_tag="ACG8KN_000439"
     CDS             46218..47552
                     /gene="rpoN"
                     /locus_tag="ACG8KN_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9187523.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47701..48792
                     /locus_tag="ACG8KN_000440"
     CDS             47701..48792
                     /locus_tag="ACG8KN_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFP9187524.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            48833..49834
                     /gene="gap"
                     /locus_tag="ACG8KN_000441"
     CDS             48833..49834
                     /gene="gap"
                     /locus_tag="ACG8KN_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9187525.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
BASE COUNT        16514 a         8669 c        10556 g        14190 t
ORIGIN      
        1 ttatacaaaa agggtcataa tttcaagtaa gcggatgaat tcatgaggat tttttgcata
       61 ctgtctctac tatacttagg tcactaagaa aaaggagtga tttattatga aaaagaacat
      121 gattgggaca ttattacttt caacacttct tctaggcggc ttggcaacac cagcttttgc
      181 tgagggtcaa gcgacttcaa aaggagatat tacctttact gaaccaacaa ataccgttga
      241 gcctcttaac cctactgatc cttcaaaacc agtagagcca gcagatccgg aaaatccagc
      301 aactggccag acaggttctt taacattgga tgtagttcct gaattgccat ttggtacaca
      361 cgagattgaa agcggaacaa aaacgtatca agtagacgct tcaaaaaacg atacacctta
      421 tctccaagta tcagatcgtc gaggtgtagg tgcagatggc caagcacaag ggtggaacgt
      481 gacagtttcg gtttcagatt ttgtaaacgg ttcacaagtt ttgcaaggcg ctgaattaga
      541 ttttggaaca tccactgtta aaagtacgtc agataacgaa tccactggac caactagtca
      601 aactgtaact gggttgagta aagcaagtgc agcgacacca atatttacag cagcaaaaga
      661 tcaaggtctg ggaacatggc tttctgttta tgatccagca aatatcacac tgaaagtgcc
      721 gaaagcagca gctggaacat tcacggctga tttgacttgg aacctcgtgg ctggcccagt
      781 agcatagagt atgaaataaa ttcaacaaac tgcggtgaaa atactatagg tgaaggattt
      841 tttaccgcag ttgttgttga aaggatgtgg tggacaatga atcaaagtaa acgactgttt
      901 gtttcgtttt tttctatttt aagtctattt ttcatttttc cgtcaatcag ccaagcaaag
      961 gattcagcag gagactttgg aatcaaaccg gtatttccag agaaccaaat agataaagca
     1021 atcggttatt ttgatttatt agtggcaccg gaacaaaatc agacactaga agtaattatc
     1081 agcaattctt cagatgaaga acgaacattt gaggtctctg tcaatccggc ggtaacgagt
     1141 gatggtggaa cgatcgatta tagtcaaaaa aacccaacgt tagatgagac attgccgttt
     1201 gatgtccgtg acgttttgct cattgcgaaa aaggaaatca atgtatcagc acatgcagaa
     1261 acaactatac ctatagaagt gaaaatccca gcaaaaagct ttaagggacg tgtattagcg
     1321 ggtattcatg tttcacctaa agaagaagct gaaacagaaa atgctacaga aggagcacaa
     1381 atcaaaaatc gtattgctta taatctcgca gtcgttttac aagaaagtca agagacgata
     1441 gaaccggatc tgaaactttt atcaggagat ttggacgagg tcaacgcaaa gccaactgtt
     1501 cagcttcgtt tccaaaatcc tcagcctagg attatcagca atttgatatt tacgagcaag
     1561 atcttttatg aaaatcagct ttatattgaa aatacgtcta atgaattttt agttgcacca
     1621 aattcgaatt ttcacttgaa tcttgattta gctggtgata aagcaaaagc cggcgattac
     1681 cgcgcagaaa tcatagcaaa atcaggcgac agtaatgaat ggcgatttac acaaaacttt
     1741 acgatcaaaa aagaaaaagc acaaaaagta aatgaaaatt ccgtttttgc agtacaagaa
     1801 caatctttcc catggcttta tgcaggattg gttgctgcag ttctagtgtt atggttgatt
     1861 ttgctgtttt tgtgggtcca caagaaaaag aaaaaaggag aaacaacatg atgaggtgat
     1921 ttcgttgaaa acatcgatct tattgggagg gattattgca agtttgctgt tcttcagctg
     1981 gcaagagcca gtccaagcag ataccaaaag ttcaggtacg attacatttt atgatctaag
     2041 tccagttccg catccaagtg gttcagtcaa tggcgcatca aaacagcaga cacagctacc
     2101 gatttataaa gaatctaatc aacatggcgt attcccggag acgaatgaag aaaagatccg
     2161 ttttctttat ttgatcattg gtatagaatt atggcttctt gcgtggttgt tgttatggag
     2221 aagaaaggaa aaggaggatg aacatgttgc tacaaaataa attttcattt tttcgctcct
     2281 tcttttttac ttcgcttttt gttttaagta tatttatctt ttcaccttcg gcagaagctg
     2341 ttggagcaac gcctcctgcc atttcttatg atccatacac gtatgaaaat atcgtacctc
     2401 caaatggtgg acgtagtgca gtcaatggcg ggaagcttgt ccaactaact ggggctaacc
     2461 aattgcctaa attgactgct acttatttga acgatggttc aaatgatcct tggaaaggtg
     2521 gcacggtccg ttttcattgg gaaagccatg tgaaactaac aaatgtactt gatttggtga
     2581 atatcactgc acaaggattt gcatttgtgg cgatcatcaa tttgccggaa catgtatcaa
     2641 gcagtgatgt attaaaagca attgactgga acaacgccta cttgtatatt gccggtgccc
     2701 ccatcaaggt tactagcggt agtttattat cagcaggaag ccataccttg cgttttggga
     2761 tggggtcgtg ggatacaaat actctattgg cagatattat tgctattatt acgcgaggcg
     2821 aattcaacgt ggacgatatc ccgcttcaat ttgatgttac agctaatgtc gcgaagatga
     2881 cagaaaacgg agataaatat gatacgagtc caaacaaaat tttgaccaag ggtaaattac
     2941 aaccttctat gtctaaaaag gccgtttttt ctgttgattt ttatgattcg aataatatta
     3001 tccaagatcg tatgttcaat tcgattttag ctccagctgc aggtagattt tatcctaaat
     3061 taactggcgg aaacttgaat ccaggagcat atgcattttc tgcaagtgca gagataaata
     3121 cgtggaatag ttacatctca acaagtgatt caacaggaaa atataataca tttgctgata
     3181 ccacagaata cgtggatgga aataaccaga accttccagg ctctaccaat agtcggacat
     3241 tgacgatggg gctgactgaa ggacgttttg atcagttgcc atttagtcgc tttgatcgcg
     3301 tagtcaacta ttttacagga aaaaacgtta cacaaggagc aatactgact catactccag
     3361 caagcacaag tggattgaat cagacagcca atatcgtgta cagtgggaaa gatgcgcaag
     3421 gcggattgct ttctcctgtt gctttaaagg tattggagca gtacaagcta gacggaacgg
     3481 ttttaccaac aaacttaaca gccgatagtc agtggggaaa gctcctttct tctgcatcgt
     3541 accaagtcac ggccaattgg tcagcaactg aactaaaaaa gggttcgatc cattacaggc
     3601 tttattcgaa aggaacccaa aaattagcga caggatggac agatcaactt ttccaaacga
     3661 tccaatcaaa taacgggggt gtaaatacag ctacttctac actacctgca ttggcttcag
     3721 ggcaatatta ttttgattat cgcttggtgg acgatgtact tactaacatg tatccgtctt
     3781 ttgcgtacaa atggcagtct gaacaagctg gaatcaccac tcttcctcaa ataacagtgg
     3841 ctaattttcc atctgtgagt gcagatagtc agctaaaaaa ccttagcaga caacctgaga
     3901 caggagatcc cctgtctgct ctatcaggag atacgattca agaaaatgtg acattcacgt
     3961 taacaaaatc tggggatgcg ataaccgaca aaaaaatcac gatctcactt cctgaaaata
     4021 ctacttatat caatggttcc ttaaaattaa atggtacaac tatcgcagat accggcattc
     4081 aacaaggagt ttctgtccca gcagatctat tgagcaaaat aggagatacc attcatctta
     4141 cttacaacta tcaattaaat actgtagata cttctgtaca atccgtcagc atactgacaa
     4201 aggcagctgt actttccagc aatattacat tagcagatgg tgctaagctt cctaacccag
     4261 ttgtccaaac atctgctaaa acaattcttg tccccaaaca agaattaaca ttagttaatg
     4321 taccagatga ttttacattt ggtaacgatc tccccaaacc gttgaagaca agttattatg
     4381 aagcaaaagg cgatttttct tttgatgtgc gggatactcg gcttccaagc acgtctcctt
     4441 ggcaactgac agggacattg acttctttat ttaagaatga tcaaggtcaa gaattaagcg
     4501 gaacaaaatt atacttcaat cattctggat caaaacaatt gattcagcaa ggtcaaaaca
     4561 cgttgattta tgagagtgat ggaacagcta aaggagaggt tctcgttgac tttcccgata
     4621 ccgatggttt actcttagag gtaaatagca gtacgaatgc tcaacctggc gcaacgtatc
     4681 aaggcatggt cacatgggaa ctgacagctg ggccaaccag ctaagtgatt agaatttgta
     4741 tggatctgtg ataactgtca cagatcctct ttttcaagga tgagaaggaa acttctgctg
     4801 ttaaaatcag aaatgcttgg aggagtggac ttggaacttt tcagtttgct tgaaaaaaat
     4861 gaacaacggc aaatcaaact tttccaactt ttattagagg aaaaaggaga gatttccgtt
     4921 aataacgtac ttacccgctt atcagttgat cgggctacct tgaaagaaga tctgttttat
     4981 ttacaagcta acttggaaaa tatcaaacaa ttggaaattg ttatccaaga agggaatatt
     5041 ttattaatca gacatggaaa tatagcagca gtcgatgttt attatcattg ctacttgcgc
     5101 tattctacaa aatatcagat acttctttat ctgttggaaa atggctcatt tgatcggcag
     5161 aaacttgtca tggaacttaa tatcagttcg gctactttgt ctcgcagagt gaaggaatta
     5221 aatgattctt taaatgagtt tcatctgaaa ataaaaagcg ggcggctgac cggtccagaa
     5281 agtcaaattt gttattttta tttccaatta ttttggtttg gacgtcctta tcttgaaaac
     5341 aaaaaagaat acagtgattt atcaggaaac acttttttag atctgctgca aaaagagttt
     5401 agttttacta ttacagaaga aggacgattg aaatttgaaa tatggctttc cattatgaag
     5461 aagcgaatgc gctgtacgaa aaaatccaaa ccagattata gcaatcttcc tgttggattg
     5521 tttcaaacga cacaattttt attggacctc catacatttt tgacccgtta cttctttcaa
     5581 tatgcctttc cttggaatca aaatgaaacg ttggtttttt atcttttcat gatcagtaac
     5641 tttacattag atacacataa ctcgcatgtc caggagtttt tgataagcga aaaatacaaa
     5701 gacgacgtag tagctcaatt gaatcgaacg tttaaacaat cgatagaaga atatttctct
     5761 ttttatgttt ttccagaaga gatttcgcaa aagattgcgt tgacagtgat gcaaaatcac
     5821 gctcattttg cctattttag aggatggatg aatatttttg gccaagaagg attaaaacag
     5881 cggttaactg aagtagaaga taaacaattc ctggttttat gtgaaagatt ggtcagacaa
     5941 acatttgcta ttgttgattt tactgaagta gacgaggaaa atgcccgctt tgaattattt
     6001 gctcgctatg catctatttt gcggacgctg atcaagcagt tagaattacg tctgcaaatt
     6061 gcttatgatc ttccttatga aaggctaatg gcggatatcg tgaaagaaat catccaggaa
     6121 caattgctga atacgatcaa atatgattta gtcgaatacg agaaagaaaa gcagtatgat
     6181 atcattttga ctagccaatt gaaagagtac ccgtcacaga agtccgctaa agtttttgta
     6241 ttcactagca acgaatataa ttatgatttc ccctatttga accaattttt aaaagaatgt
     6301 tatttagaaa aattgaattt aaggatgaat aaaacatgaa aatcgtgatc attggtgctt
     6361 cccatgcagg catcacagct gccctaaatc taagaaaact tcaaccgaaa gcagaagttt
     6421 tactgatcga tgaagatcat aaagatggac taggctatgt ttcaaatgga atcaaccttt
     6481 atttaaaagg aaagatacgt agtttagctg aagttgccca taacatgcgg actcttcaag
     6541 aaagtggggc aaaactgatc accgaatgga gagtaactga acttgatcca gacaaacatc
     6601 agctcatcct cgcatccaaa gaaggaaaaa acgagaccat aacctatgat aaattaatcg
     6661 tagcaacagg ttcctctcct gtcaccttgt ataaacaaat tgaagctgaa aatgtctaca
     6721 cctataaaaa tctagtgcaa tctaagcaag tacttgccgc tttaaaagaa gcaaaagaag
     6781 tagtgatctt cggtgctggt tatattggat tggaattagc agatgctttg agaaataaag
     6841 gatacattat tcatctagtt gattatatgc cgaatgtttt atcaagatat ttcgataagg
     6901 atatgatcaa cagttttcaa aatcagctgc aaactaagca aatcaatttt tatccaaatg
     6961 aattcttgat cgattggaaa aaatcagaag agaaagtagt atccgtacaa cttctatctc
     7021 aagcaataaa ggcagatatg gtcatttttt ctgcccaaac tcgtcctaat acgacattgt
     7081 taaaagaaaa agtggctttg tatgaggatg agactgtgat agtcaatgaa tatctgcaga
     7141 caagtgatcc ggacatctat gcgattggag atatcgtccc agtcagtttt gacaaaaata
     7201 aacggcatct ctttttacct ttagttactc gagctgtaca tatggcaaga gcagtagcgc
     7261 taactttgag tggtcagcca accgcttacg acctcagaca aaagataaca gcagcagtca
     7321 ttatagacta ctttttagga actgtagggc tgacagaaga tgaagcgcca ttcttggaac
     7381 aaaataccgg ttcgtgttca ggagaatttg atctatttcc acaatacgat gaagaaaaca
     7441 aaacagtaaa tgcaaagctg atctaccatc cggatacatt agaaataatt ggcggacagc
     7501 tgataagcca agaattctta ttatcagatt tgaacctgct tgcggatatc gtgaaacaca
     7561 aaactacgat tccacagcta gcagttgagg attttggatt tctgtctgaa cacacctctc
     7621 gttttcacta cctcaatgag ttagctttta aagtactagc taagagagca aatagcaata
     7681 gagtagataa aagactgtga gaaatatcac ggtcttttat tgtgtgggga tataataaat
     7741 attaatcaat cgagaataaa aaattcatat aatgaaatag cggaagaatt ttttgagcat
     7801 aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt atttcaggag
     7861 gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac gttatgcgtt
     7921 agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg ggttagctta
     7981 tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt cgattgtgct
     8041 ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga tagcagaaga
     8101 agtcgaaggt gggaaagtgg cgtatcttgc ccagacacct ggagtcgtga ctagtaaaga
     8161 cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa atcaattcat
     8221 catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag taggcgatat
     8281 tagtttgaaa gaagaatatg gtctagccgt aggagataca gttaagttat ctggtagtga
     8341 caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct caccagttct
     8401 ctatacaacc atcaatgctt accaagagat ccgcttcgaa aaagaagata caagtgaaaa
     8461 tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg aagatttaga
     8521 acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc aagtattgac
     8581 ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg ggattttcat
     8641 ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac aagggatcac
     8701 aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat ttgttggtat
     8761 tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg cggttccatt
     8821 ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgctggtag tagctgtgtt
     8881 aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg caattggata
     8941 agaaggagga agagcatgaa agcaatagaa tttaaagaag tagaaaaaag tttccaagat
     9001 ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa aggggaattt
     9061 gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt ggcaggcgga
     9121 ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag taatcaaaaa
     9181 gaaagtgaac gcgttaaact acgttttaaa gaaattggct ttattttgca agcttccaat
     9241 ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat caaacatgaa
     9301 catcgtcaaa aagaagcaaa agaattattt gagcagcttg gcgtggataa gttgatcaac
     9361 aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc tcgtgcattg
     9421 tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga ttcagaaaag
     9481 gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa agcaacgatc
     9541 atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat gatggaagat
     9601 ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc atcgatacaa
     9661 caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt ccaagggata
     9721 tctcggcact tcaacaagag agatcgcaca aaaagttggg atcacccaac ccaatctcta
     9781 tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc acttaaaaga
     9841 tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc aagtaacctt
     9901 gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc tgatgcttca
     9961 cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc tatggaagaa
    10021 aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc taagaaatgg
    10081 gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt acatcactcg
    10141 gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt tatatttgaa
    10201 tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaaatagtta ggtgaagaag
    10261 caagatatga cgaagatcgt tatatcttgt tttttttata taggtagttt ttcataaata
    10321 caaatttgtt attctctcat gattatcagg gtatattttt atttattatg tcggtgttcc
    10381 aggaagtgtt ttaatttaat aacgaagaaa ttaattgttt gtagaatgaa ggaaactatt
    10441 tattgggtag atgaatcacc acgaatagtc aaaaggagat ttcaggatat gccaaaaatg
    10501 aaaaaaatca ttttagcagt aatgttattt tctacggtta tatcttttac agcccctttt
    10561 ttgcgtactt aagctgattt taagttttga cccttagtct ggatctacga ggtgagagtc
    10621 aagaatgaaa aaaataattg gagcttttgt atttgcaatc attatcatta ttatctgttt
    10681 tcaaatagat gtttttggtc aaaaaaagat agttggacaa acggtcagca gtacgaacag
    10741 tacggttaca aagacaaagg aatcatttga tgatacagaa caagagacat taatcaaaat
    10801 cggaaaaaat gaccgtacac tatatacaga tgactcgcta actaccccac ttgcaactat
    10861 aaacggcgga gagctggcgg agtttctttc tgaaacaaaa aatgcttatc agatcaagac
    10921 aaatgatggc tatactggat atttagccct cgaagatgga accaaagtca caaagaatat
    10981 acaaacaaaa ccggaagagc tatctgatgc agtgattgta ttagatcctg ggcatggcgg
    11041 aaatgatact ggggcattaa gcaatgatga acaaacagaa gaaaaagata taacattaag
    11101 tacagccata aaagtaaaaa aggcgctaga ggatgctgga gcaacggttt atttgaccca
    11161 tactacagat gaattagtac agcttggaga tatatgcgac tacagtgaag aaaaaaaggc
    11221 agatgtcttt atcagtttac acgcagactc tacggaatat gcgaatgaag caactggaat
    11281 cacgacatat tattactatg gacaagagga aacattagca cagacaattg ctgactcctt
    11341 cactgatctg cctctggatt caagaggaat ctccactggg aactatcaag ttcttcgaga
    11401 aaatcttcaa ccttctattt taatcgagat gggttatatg aataatgata gtgatttaaa
    11461 ggaattagta acagacagtt atcagcagca gattgctgca agtttgacac aagcattgac
    11521 cacttatttt cagtaataaa taagcccagc caaacaaaaa tagctcctca tatttttcgt
    11581 ttggccgggc ttattatcgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg
    11641 agcctgccgt ttaactccgc gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg
    11701 atgactattg tgacttgtga ccgaggagtt ggcatgagaa caccgtttat tcggaacaag
    11761 ggactgtgac atgacttttg tcacagttcc attatttttt tagcagcttc aaagcttttt
    11821 ctgtattggc aaaatgaagt ttggcatatt tttcaagcat aggcatgccg cctttctcca
    11881 atagttttgc cgctacttgg tcagatttcg ttccagctcc tgatggaagg gtgattccag
    11941 cttcagctga tgctttgtgt aactcttcca aaaagctggc acgactgatg atagaagcag
    12001 cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt tcttgtaatt
    12061 gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac tgatcgatca
    12121 aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc gcttgattgt
    12181 gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg tatttttctg
    12241 gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca agctggatga
    12301 tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat tctgcaagct
    12361 gatcacgttt cacataagca gcacatactg ttagcggacc aaaatagctg ccatttccga
    12421 cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa gaggctgttt
    12481 ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct attgctgcat
    12541 tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc ccgtcttttt
    12601 ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga tagtatgctt
    12661 tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata aaattctcct
    12721 cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa gagattgcga
    12781 gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa atatttgtgg
    12841 agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca aataggagga
    12901 aatgattcgt atggcgcacg aaaaaacacg ctacaaagca gtgattgcca accaaacata
    12961 tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc tgatcaatga
    13021 acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag cagcaatctt
    13081 gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatct tggagctgga
    13141 agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg aaaatcgtgt
    13201 gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa atggtcaagc
    13261 agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga aacgaaaagg
    13321 acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa aaatttaaaa
    13381 aagttagaag agaaaggaac gtagcatgct gagtttgctg attttatttt tattgctaat
    13441 tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa atcatattcg cgattggata
    13501 tgtattgtca tttattgctg cccagcattt ttacaaacca ttagcaagtc atttggaatt
    13561 atacattcca tacccagctg tcaccccaac atcaaagtta gcattttttg atcaggtatt
    13621 tgcttttcac ctagatgaag ctttttatgc aggaactgct tttctgatta ttttgctaat
    13681 tggctggctg cttacccgat ttgtgggggt ttttgtccat ggattgacgt atgtgccgat
    13741 cttgagacag gtagactgga ttgcaggagg catcctgtca ctaatcatgg cttacgttac
    13801 tatatttttg attttgcagt tacttgcctt tgttccatta gatgtggtcc aaaatcaatt
    13861 cggcgagcat agtttcgctc gttttatcgt tgagagaacg ccatttctta ccaacaagat
    13921 gtatgagctt tgggtaacga atattttgaa ttaaggaaaa tagtgctgga aaatatttcg
    13981 ttttttgtac agagtagttt atttaagaag gctgcgtagc gtatgtccgg cacagctctt
    14041 ttttcatagt gaaagataag gtgataaaat gaataaccga atccttgaaa cattggaatt
    14101 tgaaaaagtc aaacagatgg tcagacaatt tgtagtgact gcacaaggaa aagaagaact
    14161 ggcagaattg gtaccagtga gtgagaaaca aacgatcaca aactggcttc aagaaactga
    14221 ggatggattg aaagtgcaac gtctgcgagg cgggatccct atacctaaac ttgaaaatat
    14281 ccgtccccat atgaaacgga tcgagatcgg agcagacttg aatggagtgg agcttgcaca
    14341 agtttccaga gtattgtcta cgacaagcga attgaatcgt ttcattgatg atctttctga
    14401 cagtgaaatc gaatttgcac gtttgtatat gtgggcagat cagttagtca cgatccctgt
    14461 tttgagccgc cgtttaaagg aagcaatcga tgaagacgga cgcgtgactg atgacgcttc
    14521 tcctgagtta aagagcataa gacagaacat tcggcgaagt gaacaagctg ttcgtgaaca
    14581 gttagatggg atcgtccgtg ggaaaaatgc aaagtattta agtgatgcga tcatcaccat
    14641 gcgaaatgac cgctacgtta tcccagtcaa acaagaatac cgcggtgtat ttggcggtgt
    14701 agtccacgat caaagtgctt caggacagac tttatttatt gaaccaaaac aagtggtgga
    14761 tttgaataat cgtttgcgtc aataccaaat cgctgaacga aatgagattc aacgtatttt
    14821 aagtgaactt tctgctgaac ttgttcccca tcgtcaagaa atcatccaca atgcttatgt
    14881 gattggtaaa atggatctga tgaatgcaaa agcacgattt ggtaaagaag taaaagccat
    14941 cgtgccggga atcagtgaag acaatcatgt cgtcttgaaa caagcaagac atccactgat
    15001 cgatcaggaa aaagtagttt ctaatgacat cacaatcgga aaagactatc aagccatcgt
    15061 cattacagga cctaacacag gtgggaaaac gatcacgttg aagactttag gtctgttaca
    15121 actgatgggg caagcaggat taccgattcc tgcaggtgaa gaaagtcaaa tcggtatttt
    15181 tgaggaagta tttgcggata taggagacga acagtcgatt gagcaaagtc tatcgacctt
    15241 ttcttctcat atgacgaaca ctgtagatat tttatcaaaa gtcaatgaaa aaagtctggt
    15301 attatttgac gagctcggtg caggaacaga tccgcaagaa ggtgctgctc tagcaatcgc
    15361 aatcttagat gatcttggta aaaagtctgc ttatgtgatg gcaacaacgc attatcctga
    15421 attgaaagtt tatggataca atcgggcaaa tacgattaat gccagcatgg agttcgatgt
    15481 ggatacacta agtccaacgt atcgtttgct gattggcgtt cctggacgaa gcaatgcgtt
    15541 tgaaatctct agccgattag gtcttgatac agaagttatt gatgaagcga agcagttgat
    15601 gaatgatgag agccaagatt taaacgaaat gatcacagat ttagagaatc gccggaaaat
    15661 ggcagagacc gaatatctgg aaatgcgtca ttttgtttct gaggcacaag aacttcacga
    15721 tgatttgaaa gaagcttaca gctatttctt tgaagaacgt gaaaaagaaa tggaaaaagc
    15781 aaagaaaaaa gcaaacgaag tcgtgtcaga ggcggaagaa aaagcagaaa aaatcattgc
    15841 tgatattcgt aaaatgcaac agcagattgg gcaaggaaat gtgaaagagc atcagttgat
    15901 cgatgcaaag acccaattag caaatctaca tcaagaagaa actttgaaga aaaataaagt
    15961 attgaagaag gcgaaagaac aaaaaacgtt gaaaccggga gacgaagtgt tggttacgac
    16021 ttacggccaa agagggacgc ttttacgtaa aaacgggaat cagtggcaag tagaaatcgg
    16081 tatattaaaa atgaatgtgt ccgaagacga gctgacgcct gttgctcctc aaaaagagcc
    16141 gacacaacga gttatccatg ctgtacgatc tgaatctagt agtcacgtgc cgaatcaatt
    16201 ggaccttcga ggaaaacgat acgaagaagc gctaagtgag gtagatcaat atttggattc
    16261 tgccattcta gctggttatc ctcaagtgac tattgtccac ggaaaaggaa caggcgcact
    16321 tcgcaaggga attactgatt atcttaaaaa ccatcggagc gtcaaaagct ttgagtttgc
    16381 accggcaaac caaggcggaa atggtgcgac aattgttaaa tttaagtaaa gcttactaaa
    16441 ggtaagcaga tggttcgcta tccaagtata gaaatgctat actaatcata gattttaaaa
    16501 atggaggtct ttgatatgtc acaagtaatt acagataaga catttaatga agaaacagat
    16561 aaaggacttg tattgattga tttttgggca acttggtgtg gtccatgtcg tatgcaagca
    16621 ccgattttgg atcagttgga acaagaatat gatgaagaag aattcagaat tgctaaaatg
    16681 gatgtggatg aaaatccaga aactcctcaa caattcggta tcatgagcat tccaacatta
    16741 atgttgaaaa aagatggtca agtagtagaa aaagctgttg gtgttcattc aaaagaacaa
    16801 ctacgtcaaa tgatcgatca atatttataa aaacttacag caaaaataag ctgagggtgg
    16861 gactgaatcc agttccgtcc tcggtttttt tgcggttgag ttaaggttat tcaaattaat
    16921 attttttgag tataattacg ttgtcaggct ggattgtttt tttgacagac atgctataat
    16981 aactttaatt gaatatgaag gggagtacca gatgaacgaa cgaatcaaaa acaaattagc
    17041 tttacttcct gatcagcccg gctgttactt gatgaaagat aaaaatggaa cgatcattta
    17101 tgtcggcaaa gccaaaatat taaaaaatcg tgtccgctca tattttactg gcagccataa
    17161 tacgaaaacg gaacggcttg ttagtgaaat cgaagacttt gaatacatcg ttacagaatc
    17221 gaatatcgaa gcattgctct tagagatcaa tctgataaag aaaaatgatc ctaaatacaa
    17281 cattatgtta aaggatgata aaacctatcc tttccttaag ataacgaatg aaaaatatcc
    17341 ccgtttggtg attactcgta aagtactaaa agacaaagca ctgtattttg gaccatatcc
    17401 cgatgtcggt gcggcgaatg agacaaagaa gatcctcgat cgattatttc ctctgagaaa
    17461 atgcaaacct agtcagacga aggagccttg tctttactat catttgggac aatgtctgtg
    17521 tccttattat ttcgacgtag atccgaaagt ctatacgaaa atcgttgatg aagtgaaaaa
    17581 gtttttaaac ggaggacacg aaaaaatcga gtcagagatc catcagaaaa tggaaaaagc
    17641 tgctgaaaac atggagtttg aaaaagctgc agaataccga gaccagatcc gtgccattga
    17701 gacaatcatg actcgccaaa aaatgacaaa tactgattta ctggatcgag atgtatttgg
    17761 ttatgcgatt gacaaaggat ggatgtgtgt ccaagtgttt ttcgtaagac aaggaaagct
    17821 aattgaacga gatgtttcaa tgtttccttt ctataacgaa gcagaagaag atttcttgac
    17881 ttacctcggt cagttttatc aggaaaatga gcatttcatt cctaaagaag tcttgattcc
    17941 tgatacgatc gacaagccca gtgtagaagc tttgttagct acgaaagtgc tgcaaccaaa
    18001 acgaggagaa aagaaaaaat tagttgaatt ggcgaataag aatgcctcag ttgctttgaa
    18061 tgaacggttt gatctgattg ccagaaaaca ggaacggacg atcggagcag ttgaacgttt
    18121 aggaaacgcg atgaatatcc cgaccccaat acgtatcgag tcttttgata actctaatat
    18181 catggggacg aatcctgtgg ctgcgatggt cgtattcatt gatggaaaac cggcaagaaa
    18241 agaatatcgg aaatataaaa taaaaacagt caacggaccg gatgattatg catcaatgag
    18301 agaagtcatc tatcgcagat attcacgagt gattcgcgaa gggttgcctt tacctgattt
    18361 gatcttgatc gatggaggaa aaggacaagt agatgtggca aaagatgtct tagaaaatca
    18421 attaggaatc gatattccta ttgctggcat ggcaaaaaac gataaacata aaacaaatga
    18481 gttgctattc ggtatagatt tggctgtagt accgcttgag cgaaattcac cagagttttt
    18541 cttactccag cgaatccaag atgaagtcca tcgattcgca atcactttcc atcggcagct
    18601 acgcagtaaa aacagttttg cttcccgatt agatgaaatt gaagggttag gaccaaaacg
    18661 aaaaaaaatg ctgcttaaag agttcaaatc gttgaaaaat atcacagcag ccacgacaga
    18721 agaattacaa gaaatcggac tgccgaaaaa tgtcgcacaa aacgtatttg ataaattaca
    18781 tcaggaatga ggaaagggca ctttacaaag aaagtaaagt gtcctatcat tttatgtaca
    18841 acaaaaagag cgtttacgtt ttgttggtaa ataataaatc gcttacgtct ggtgtctaag
    18901 gatatatgtg atttcttgaa gaaggagtga agaaatgtta gaagtcaagg atcttgttaa
    18961 aacttttggg aactacacag ctgtcgatca tgtctctttc caaatccccg atggaaaaat
    19021 catggggcta attggccaaa acggggctgg gaagacaacg acttttcgtt tgatcttgga
    19081 ttttttgaca caagaccaag gagaagtgtt atggaatggc catcatcttg gggagaaaga
    19141 ttatgatgtc atcggttatc ttccggagga acggggactt tatccgaaag taacgattca
    19201 agaccaactg ctttattttg cagaacttag agggaaatca agaaaagaga ttgaaccaaa
    19261 aatcgatttt tggatggaaa aatttcaggt aaaagggaaa aagacggata aagtaaaatc
    19321 tttgtctaaa ggaaatcagc aaaaagtcca gctgatcgcc acgttaatcc atgaacctaa
    19381 attgatcatt ttggatgagc cattcagcgg gttagatccg gtaaatgctg aattgctaaa
    19441 agatggaata ttggaattga aaaaacatgg ttcatgtgtc attttttcta gccacaatat
    19501 ggataatgta gaaaaaatct gcgatcattt gatcatgttg cgaaacggaa aaatggtttt
    19561 gaatggaaaa gtacatgaga ttcgtgaatc atttggccgt acgaagttat tcttggaatc
    19621 tggtttaagc caacaggaag tagaagaaat cgaaggcgtg aaaaaagtga tccttcatga
    19681 agacaatacg ttagagatta ccttagaaga tcctgaagct gggaaagaaa tctttactcg
    19741 tgcgactcag ttcggctaca ttcctatgtt taaccagcaa ccgccaactt tggaagagat
    19801 tttcaaattg aaagcaggtg aaatcgatgc gtaagttttg gattattacc aaagatgtct
    19861 atttaaaaaa tgttaagtcg atttctttct tgatcatgat attagtccct tttatcatgc
    19921 tgggtatcat ttatgtagca ggaaactttg ctcaacaaaa tagtgatgtc aataaaatcg
    19981 gcgtattggc tgataatgag caaatcagtc agcaaatgag ccaagtcaaa acagatgatt
    20041 ttgagttcaa agcagttgat tctgaagaaa aagcacaaaa agagctttcg gaagaaaaaa
    20101 tcgatgcgta tttagtagtc tcaactgaga atgataagat aaatgggatg ctttatagcg
    20161 aaaattcatt aggacaaagt acacaactac tgatacaaca acaactgact ggcttgcaat
    20221 cgatgcttcg agcaagtcaa ttaggcattt caccagaaga agtagctagc ttgagccaac
    20281 aagcaagttt gactcgacaa aaagtcaatt ttgattcaaa aggaaaaatg acagttggag
    20341 aagataactc gacagtccaa tatgtcgtaa gttatgtagc aacgattgtc ttgttcatta
    20401 ttattttgac gtatgcgcag atcattgcac aagagatcgc ttctgaaaaa ggcacgcgga
    20461 tcatggaagt gatcctgtcc agtacgactg cacaaaagca tttttacgga aaactaacag
    20521 gtgttttact tgttgcatta acgcaaatgg cattatacgg tattattttt ggtataggat
    20581 acagtcaatt caaagatatg gaaatggtca aaaatatatt agacggtatc tcattggata
    20641 acttgattgg accattcttg tggtattctc ttctattcat gatctttggc attttgattt
    20701 tttctgtttt agcagcttta tgtggctctt tggtcaacaa agcagaagat acagcaaaag
    20761 caattttgcc agtgacttat ctatctctag gcggatatat gcttggattg atactaggag
    20821 cttcagatcc aaacaatatc gttattcgga tcacttcgta tattccattc ttatcttcat
    20881 atattatgcc ggttcgctta gcaaatgaga cagtcggtat gggtggagca ttgatttcct
    20941 tgttggtact tgtagccgta acattcttat tgatgatctt gtctgcaaac atgtacaaat
    21001 cgaatgtact cgtgtatagc gaaggcggat tatggagttc attgaaacaa tcgatttcaa
    21061 ttatgagaaa tgaacgaaaa aaagcataaa atatagaaaa atatagaagg aagctggaca
    21121 tttattgttc ccagcttctt ttttgtatag acctatttct atttttttat tatattggat
    21181 atatattcat tgaaaataga gcataacaac tttataatgt tgagtatacc aatttttgga
    21241 ggcgttttaa tggaccgcta tttagattta ctttcagaga agttccctac aacggaagat
    21301 gtcatcacgg aaatcatcaa tctcgaagca attttgcagc ttccaaaagg aacagagtta
    21361 tttttgagcg atatacatgg cgaatttcct gcttttgatc atattctgcg tatcggttca
    21421 ggaaatctta aagaaaagat caaagacctt ttctctgaac aaatgacgga aacggagatc
    21481 agccaattca ctatttttac ggcttatcca gaatatgtgt tgacgactga ttggtacaaa
    21541 aaacaagata aatcccagct gatccatcgt ttgatcgatc ttttacggtt tacgacagtc
    21601 aaatacactc gatcaaaagt tcgaaaagga ttgccgaaag aatatagcta tatcattgag
    21661 gaactcttgt atatcgatga tcgtatcaac gggaaaaaag actatgtaaa aaaaatcatt
    21721 gaacaacttg tcttgatgaa ggaagaagaa aaatttttaa caaaacttgc gcagacgatc
    21781 caaaaatctg tcatcgatca tttgcatatc gtgggtgata tttttgatcg aggaactcaa
    21841 gcagccaaag taatggatcg aatcatggat ttttcctctg tagatatcca gtgggggaat
    21901 catgatattt tgtggattgg tgcgtattgc ggctctgagg catgtttatt gacgctttta
    21961 cggattgccg cacgctacaa ctatttattt gaattagaga aagaatatgg attaaattta
    22021 cgtccattat tttcttttgc ccatcaaacc tatcagaaga atccagtatt tacccctaaa
    22081 aacaggaaag atctatctga aagagagcaa gaagaattag aaaagatcca tcaagcactt
    22141 tctatcctcc aattcaaatc agaacatcag ttgttgaatc gacgacctga atttaaaatg
    22201 gacgatcgta aattgttgga aaaaatcaac tatgaggaat caacgatcgt tttagaaggt
    22261 cagctctatg agttgaaagg gacttgtttt caaacgatcc agccagatga tcctaaaaaa
    22321 ttcttggaag aggaacaaaa agtgatcgat agtttgatgt attcattcca gcactcgtta
    22381 agaatgcaaa aacacatgac gtttttgatc gaaaaagggg gaatgtatct cacgaacaat
    22441 cagcatgtac tgttccatgg ctgtatacct gtagatgaaa aggggcagct cttagcattt
    22501 gaattagacc aatcttatcg cggaaaagaa ctgctgggat tttttgaaaa acagataact
    22561 gaaagcgcaa aagatttgac tgcaaatgaa gatttggcga ccgatttgat ttggtatgcg
    22621 tggagcgggc cattttcgcc tttattcggc aagagtaaaa tggcaacttt tgagcgctat
    22681 tttctcacag agcctcatac gcacgtcgaa aaaagcaatc cctattatca cttgcgagat
    22741 gaagagtggt tcagtgaaaa aattttggca gaattcgatg caaaagaaga aggttcagca
    22801 atcatcaatg gacacacgcc agtaaaagta aaaaaaggag agtcaccgat caaagcaaac
    22861 ggaaaagcat ttgttattga cggaggtcta tccaaagctt atcaaaaaac aaccgggatt
    22921 gctggctact cattactatg caattcttac ggtttccaaa tcgtgacaca ttatccgttc
    22981 ttgcctattg aacaacaatt tgcaaaaaaa tctgatcaaa cgaatgtaaa aaaagtgatc
    23041 gagcagcagc tcccacgcaa acttaatcgg aatacgacaa aaggaatgga attacagcaa
    23101 cagattactc aactccaaag gctgttggat tatcgaaagg acgattgaga aaaatgctag
    23161 ccaacattgc tcaatagact aaaaagcgac tgcaacaaaa ggcgtgtcgc agtctccttc
    23221 ggaattagct caaaaaacgg taaaatatta gaagctattt ttctgtttat ttctctaata
    23281 ctcaggtcag ggcgctgatt tcacgacctc ttccttaaac ggtgttctcc tgccaactcc
    23341 tcggtcacaa gtcacaatat ccaacaaaca tgagaagctg ttttttgaaa ttgctccttg
    23401 tgactcagag ttgaacggca ggctcacgac ctaatcagaa aaacgattaa aattcattca
    23461 caaagaaatc gaataaacgc tgttcagatg aatctacacg atgagaaagt tccggatgcc
    23521 attggacacc aaggattttg tgcatattat ctttactttg gactgcttca atgagtccat
    23581 cgttggaaaa ggcgatggct tttaacaaag gagaaagatc tttgactgcc tgatgatgat
    23641 aggaattcac aaagtatgaa tcagggagaa ggtcacttaa taaactgtcc gaaacgattt
    23701 tgacttcgtg agttgcaaac tgcggggcag tgggctgttg ttcgtgtttt acggaccatt
    23761 ttggatataa tgaaagatct tgataaagtg ttccctccaa cacgacattc aatagttgaa
    23821 gtcctcggca gactgcaaaa atcggttttc tttgtttcaa tgcttccaag atcaatgcct
    23881 gttcgaattg gtctcgttga atatttgttt ccgtcagttt tggatgaggg tcttccatgt
    23941 aaagttgcgg actaacatcc tgaccgcctg caagaatgag tttgtcgatt ttctcaatta
    24001 gttgaggcgc tgaatctgga gacatgactg gcagaataag aggtaatcct tgagcatcta
    24061 aaactgcatc cacaaatccc tgcggtgtat agcttacttg atttccttgg aaagtatctg
    24121 tcgctcgaat cagttgattc ccggcaatgc cgatgattgg tttcattttt attcccccta
    24181 aagtagacta attgtaacaa ggatttaaaa aaatttaaac tatgtaagac ttgcccctat
    24241 acttatctgt tcttgctgtg ttactatctg taaagagaat tttcgtgtga aattcatgac
    24301 gatagttgaa aatattgctt aatttgttat aataaaaagt aagcagaaat aaaaaggttg
    24361 ggcgtaagct tcaaggatct agttcttcaa ggggtgagaa ggacatttta ttgaaggggc
    24421 ctgcctatac taagttatct ttagaggtac atactttcaa tgaaaaaaga aagcatgtac
    24481 ctctttttta aatcgaaagg aagtgaagca agtgttagaa gctttggaac aaatccgaaa
    24541 agcagaagaa gaaaatgaga agcgtgctcg aaccctacgg cagcagttga agacctatgc
    24601 agaagaaaaa gagcaagaat tggagataat ccaaacgaac agtcggcaac agctgaaaca
    24661 gttggtagaa gaaaaagaac aattcgaaaa gcaagaatta gaaaaagaaa aaatgaaatt
    24721 gaaagcagaa gcagacaagg tgcaagcaac tttgaaagac caatatcaaa agcatcagag
    24781 tcaagcaatc ggtgcaatca ttgaaagggt gaaagaaact tatggccgtc actaagatgg
    24841 aaaaagtgac cctcatttca gataaaagaa atcaggagag gattttacaa gcagtacaag
    24901 gaatccaaaa tgttgaaatc cgggatctgt atcaaaatag aacaaacaac cagtgggtag
    24961 aaaaatattt tccagatacc acgattattg ataaagaagc aaaattaagc gctttaggca
    25021 atcggctgaa agacatccaa gaagctatcc aattcataca acaccacggc gatagccgtc
    25081 aaaagaaact ccatttgaaa cgtcaagaac tttccttaca agacttggaa gcaaattatt
    25141 ccgaagaaaa tttcatgaat aaattagaag aggtccttga tttaaaagaa aagtgggaac
    25201 agctaactga aaaacgtgat cgtttagaag aagaggaaga atggcttctt cattggcaac
    25261 agctggatat tgctccaagt atgtatgcaa gtaagacgac catttttgag atggggactg
    25321 tgagtgcagc aaattttgaa agtttcaagg aagaactaag taagcttgat gacgtttata
    25381 tggaagaaat agattatacg ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat
    25441 cagttgctgc tatagaagaa attgctggga aatacgggtt caataaagaa acgtatcctt
    25501 atgaacaaaa tcctaaagaa aaattgcaag aaaccaaaca gcaattatcc attgttcacg
    25561 agcagcaaaa aaagataacg acagcgttag gtcagtgcag cggctatatc caagaatttg
    25621 aatgggtaga agaagtcaca cttgccgttg cagaacggga aaaagtaaaa gaacggttta
    25681 tccatgccac ttatctgatc gttttgcaag gttgggtaga tgcagaagaa aaacagaatt
    25741 tgatgcatgt gttaaacgaa actgtatcag aagaagatat atacgttagt ttcgaaacac
    25801 caaactcaga agagattcag caggaagtcc ctactaaatt gaaaaaccat ccgcttatcg
    25861 aaccttttga attgctgact gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa
    25921 cgccttggat gacaccattt tatcttgtct tcttcgggat gatggtcgca gatgttggtt
    25981 acggattgct gatgctcatt gggacaatac tagctcaaaa actatttgtt ttaccccgcg
    26041 ggatgaaacg tttcgtaaaa ttctttgaga ttctctcggt accatcgatc atctggggat
    26101 tgatctacag ttcatttttc ggacaggcat taccaaaaga actatttggt atccgcttgc
    26161 ctttcccaat cctatctacg acggatgacg tcaatacgat tttgatttta tccgtgattt
    26221 tcggtctgat acagatcttg gttggattat tcgtggcagc aaaggaaaat atcaggagga
    26281 aagattactt gagtgctgtc agtgatggtt ttgcctggca agggatcctg attgggatcg
    26341 ttattgcatt gattggtgtc atgttgataa aaaatacagc atttgtttat cttggcgcgg
    26401 ggctatcgat cattgctgct ttatgtatct taatcgtgcc gattatccaa tcgccgtcta
    26461 aagtcaaggg tgctgccaaa ggagcgtata atctttacgg attgactggt tatatcggtg
    26521 atttagtcag ttatacacgt ttgatggcac taggtatttc aggcggaagt attggcgcgg
    26581 cattcaatat gctagttgct ttcatgccgc cagccgctcg attcagtgta gggatccttt
    26641 tgatcatcgc tttacacgca ctgaatatgt ttttgacact gttaagtgcc tatgtccatg
    26701 gtgcacgatt gcagtatgtc gaattttttg gaaaatttta tacaggaggc ggacgtgcct
    26761 ttgacccgct gaaaacggcg gaaaaatacg tcaacatcaa tcataagaaa aaaaacaata
    26821 aataattgga ggatttttat acaatgatgg attacttaat tagtcaaaat ggtggaatgg
    26881 tatttgcagt attagcgatg gcaacagcga cgattttttc agggattggt tcagctaaag
    26941 gtgttggaat gacaggtgag gcagcagcag cgttaacaac aagtcaaccc gaaaaattcg
    27001 gacaggcttt gattctgcaa ttgcttccag gtacgcaagg gttgtatgga ttcgttatcg
    27061 cctttttgat ttttatcaac ttaggaaatg atatgtcagt tgtacaagga ttgaactttc
    27121 taggcgcttc tctgccgatt gcttttacag gtttgttttc aggtatcgcc caagggaaag
    27181 ttgcagcagc aggtatccaa attttagcga aaaaacctga acatgctaca aaaggaatca
    27241 tttttgctgc gatggttgaa acatatgcga ttttaggttt cgttatttcc ttcctattag
    27301 tattgaatgc ctaagcataa aggaggaaaa gcagatgaac gccattgaaa aaatcatctc
    27361 tcagatgaat gaagcagcag agcaagaaag agcggcgtta gagcaagaag agcgaatgaa
    27421 gatcgatcaa aactttgaac aaaaacgtac gcaaatcgaa acagaacatc aaaaacaaaa
    27481 agaaaaacaa atcgaattac ttgaaaaaaa ataccgtcag cttaggaatc gccaacaagt
    27541 agaagtacga caagaaaatt tgaatgcgaa gcaggaattt ttacgacgat tgtttgcaga
    27601 tgcaatagct gaaatggaga attgggatga atctgaacaa atccagttta tcaaaaattc
    27661 attgtattca ttacctttga caggcaaagt aacattcatt gctggagaaa aatcagcagc
    27721 ttatctttct cagacattgc tggatgaatg gaacaatgaa cttcccttta tgatggtttt
    27781 aagtgatgaa actgttgctg atcaagcggg ttttctgata aacgatcaag gagttcagta
    27841 taattttctt ttcagcagcc ttgtacaaga tatccaagga acgatgagtt ttgagattgc
    27901 caatcaatta ttcgaataag gagggatcag tgtatggaat accacgaact aaatcccttg
    27961 attcgcggaa gagagcttga acttttgtcc aatgagacat ttgaacggat gattcaaaca
    28021 aattctgtcg aagcacttgg tgaaatattg aaatccacga tttacagtcc atatatttat
    28081 gacggatatg aatatgactt cgaacagaat cttttaaagg agcagagccg tctatttcgc
    28141 tggttaaagg aatcagcacc ggagcctgaa atcgtttgga tctatacgat gcgctatact
    28201 ttccataatc tgaaggtttt gacaaaagcc gagatgacaa atcaaaatct ggaccatttg
    28261 tatatcgacg atggatttta ttcattagaa accttgaagg atgccattca tacgcaagtt
    28321 tcaaatgaac tccctgaaag cattatggac agtatacgag aagtacgtga ttattttgaa
    28381 gagtctacca ttcttcaggg aattgacgtg atttatgatc gacagttttt gacagagcag
    28441 cgacggcttg ccgataagct aggatatcaa gaattgctga cagaagtcat tgcttttatt
    28501 gacttgacca atatcacaac gatggctcga ggaatattgc agaaaagaac atatgggttc
    28561 atgacgacgg tactttccag ctcgggaagt atcgaaaaag aagtattttt gaactttgta
    28621 gataaagata tggaagatta cacgaatttt ctattagaga cagattatgc tgattttctg
    28681 cgccctgtca tccgtgaggg aaatcttgat ctcgtaaagc ttgaacagtt gaaagatgat
    28741 tatctaagtt cattgtatca agaagcacaa acccaggctt tcggaccact gccactacta
    28801 gcctttttga acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa
    28861 agaagccatt tcgaaccaga aacaatcaga aagcgggtga gacaagtcta tgacgcataa
    28921 gatcggtgta gttggcgaca aagattctgt tttgccattc aaattgtttg gctttgatgt
    28981 tcgttatggt acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga
    29041 gtatggcgtg atttatgtca cagagcaatg tgcagcattg agcccagaaa cgatcgaacg
    29101 ttacaaggaa caaatgatac ctgctatcgt attgattccc aatcatcaag gatcattagg
    29161 gatagggctg gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata
    29221 gtatgaaaag gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct ccggacctct
    29281 tgtaatggct gagaacatgt ctgatgccag tatccaagac atgtgtctag tgggagatct
    29341 aggagttatc ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc aagtatatga
    29401 agagacttct ggaattggtc caggcgaacc ggttcgctct actggtgaag ctttatctgt
    29461 tgaattagga ccagggatca tttcacaaat gtttgacgga atccaacggc cattagatac
    29521 ctttatggaa atcacacaaa gtaatttctt agggcgcgga gttcaattgc ccgcattgga
    29581 ccatgagaaa aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg
    29641 agatgttatc gggatcgtag aagaaacaaa agttataaaa cataaaatca tggtgccaaa
    29701 tgggataaaa ggtacgatca aaacaatcga aaaaggttca ttcactattg atgaaacagt
    29761 atgtgtcatt gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa aatggccagt
    29821 aagacgtagt cggccaatca aagaaaaatt aaacccggat gcacccatga tcactggaca
    29881 gcgagtgatt gatacattct tcccagtgac aaaaggagga gcagcagctg tacctggtcc
    29941 ttttggtgca gggaaaacag tcgttcagca tcaaattgca aaatgggccg atgtggacat
    30001 ggttgtctat gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt taaatgaatt
    30061 ccctgaattg attgacccta atacaggaga atcgttgatg gaacgtacgg tattgattgc
    30121 taatacatca aacatgccag tagcagctcg agaagcttct atctatacgg gaatcacaat
    30181 tgcggaatat ttccgtgaca tgggttatga cgttgctatc atggcagact ccacttcacg
    30241 atgggcagaa gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg
    30301 ttatccagct tatcttggtt cacggcttgc ggaatactat gaacgatctg gaagagtcat
    30361 tgcattagga tcagaaggtc gcgaaggaag tataaccgca atcagtgctg tatcgccgtc
    30421 tggaggggat atttcggaac cagtcacaca aaatacattg cgggtcgtaa aagtattctg
    30481 gggtcttgat tccagtttag cccaaaaaag acattttcct tcgatcgatt ggatacaaag
    30541 ctattctctt tattcaacag aagtagggaa gtatatggat catatccttc aagctgactg
    30601 ggcatccatg gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat
    30661 cgtgcgtttg gtagggattg attccctttc agataatgac cgtctgacat tagaagtggc
    30721 aaaatccatt cgtgaagatt atttgcagca aaatgctttt gatgatgtcg atacattcac
    30781 ttcaagagag aaacaattca aaatgttgaa gatgatcttg acctttggtg aagaaggacg
    30841 ccgtgcgttg tcgttaggtg cttatttaag tgagatcatg gacggtaccg tatcgattcg
    30901 tgaaaggatc ggacgaagca aatatatccc ggaaacagaa ttagaaaaac tagatcaaat
    30961 tattggtgaa atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca tgagcaatga
    31021 ttaaagaata tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg
    31081 ctggtgtcaa atatgaagaa ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg
    31141 gacaagtatt ggaagtccaa gaagataaag caatggtcca aatattcgaa ggaacaagtg
    31201 gtatcaattt acgtgactct tctgtacgat ttttaggcca tcctttagaa ctaggtgtat
    31261 cggaagacat gatcggtcga gtattcgatg gtttgggacg accgaaagat aacggaccag
    31321 agatcctgcc tgaaaagcat ttggatatta acggagaagt aatcaatcca gtagctcggg
    31381 attatccaga tgagtttatc caaacaggta tctctgcaat cgatcatttg aatacattag
    31441 ttcgtgggca aaaattacca gtattttctg gctctggtct tcctcataaa gaattagctg
    31501 ctcagatcgc gcgacaagca acggtactcg attcatcaga tgatttcgcc gtagtctttg
    31561 ctgctattgg aatcactttt gaagaagcag aatttttcat ggaagatttt cgtcaaacgg
    31621 gcgcaatcga ccgttctgtc atgtttatga atttagcaaa cgaccctgca atcgagcgta
    31681 tcgctactcc tcgtatggct ttgactgctg cagaatatct tgcttatgaa aagggaatgc
    31741 atgttttggt tatcatgaca gacatgacca actatgcaga ggcattgcga gagatttctg
    31801 cagcacggcg agaagttcca ggaagaagag gatatcctgg gtatctttac acgaacttag
    31861 ctactttatt cgaacgagca ggaagaatcc gcggactaaa aggatcggtt actcaaattc
    31921 cgattctaac aatgccagaa gacgataaaa ctcatccaat ccctgacttg actggttata
    31981 ttacagaagg acagatcatc ttgactagag agctttacaa gagtgggatt cagccaccaa
    32041 ttgatgtact cccgtctctt tcgcggttaa aagataaggg aactggaccc ggaaagacaa
    32101 gagaagacca tgcagctacg atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg
    32161 caaaagaatt ggccgtagtt ttaggtgaat ctgctttatc tgatgtagac aagatctatg
    32221 cgaagtttgc tgagcggttt gaaaaagaat atgtcaatca aggattctac acgaaccgtt
    32281 cgattactga aacacttgat ctagggtggg aactgctatc gatgttgcca agaaccgaat
    32341 taaaacgaat caaagacgac atgctggaca aatatctgcc agaagggaag tgagttctga
    32401 tggcacgatt gaatgtgaat ccgacccgca tggaattgac tcgattgaaa aaacagttga
    32461 caactgccac aagagggcat aaattattaa aagataagca agatgaactg atgcgtcaat
    32521 tcatcctgtt gattcgaaag aacaaccagc tgcgtcaaga aatggaagag tctatgacag
    32581 aagcaatgtc tgattttgta ttggcaaatg cttcgatcac ggagcctttc atcgaagaat
    32641 tatttgtttt gccggcggaa aacgttgatt tgtctatcgt agacaaaaat atcatgagtg
    32701 taaaagtacc tgtgatgaat tttgaatatg atgaacagct gacagacaca cctttggaat
    32761 acgggtattt gaattccagt gccgaactgg atcggtcaat cgatcggttt actaccctgc
    32821 ttccaaaact tttggaactg acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa
    32881 tcgaaaagac acgtcggcga gtcaatgcat tagaatatat gacgatccct caattagaag
    32941 aaacgatcta ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt
    33001 tgatcaaagt aaaaaatatg ggtaccactg actaagtggt atcctttttt aaaagaggaa
    33061 aacgatgatt aaacaaagca aacaaataaa gaaacgcttg ccggctgttc agcttctcgc
    33121 attaggattt tttctcctga ttttagttgg cggcggccta cttaccctac cattttttag
    33181 taatagtggg catgggacaa attttatcga tgcgcttttc actgcaactt ctgctgtctg
    33241 tgtgacggga ttgacaacat tgaatacagc agagcattgg aacagtgctg gtcaattttt
    33301 gatcatgctt ttgattgaga tcggcggtct tggttttatg atggttccga ttctatgctt
    33361 tgcattgata aagaagaaag tcagtctcag cacacgtatt atattaaaag aagcgctgaa
    33421 tctagaagaa gtatcaggtg tgatgaagct catgctttac atcctgaaat tggcctttgt
    33481 gatccaactg attggtacgg ttgctttatc ttttgtattc gtgcctgaat ttggctggtt
    33541 gaaaggtatc tggtacagtt tgttccatgc aatctctagt ttttgtaatg cagggtttga
    33601 tttgttagga gacagtttag ccaatcacca gcaaaatgtt tatttgatca tggtggtatc
    33661 tgctttgatc atttctggtg ggttaggctt catcgtatgg cgtgatttac tggaatatca
    33721 tcagaaaaag aagataacga ttcattcaaa aatcgcatta accgttacag gtaccctttt
    33781 attaggcgga ttcgttgtgt tctttttcac agagcgcaat ggggaaacgc tagtggaagg
    33841 gaatatgttt caacgattgg caaacacctt ttttatgagt gtgacaccta gaacggctgg
    33901 atattattca gttgattacc tccatatgag ccatgcaggt ctgattttaa cgatgttttt
    33961 gatgtacatc ggtggaacat ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg
    34021 tgtgttgctg atccagatgc gttcgatgct taaaggacgt acgagagcgg aagcatttgg
    34081 ccgtaccatc cgacaaggcg cagtgttgcg ggcacttact ttattttttg ttacgttgac
    34141 gttgtgtgta gtcgccatca tgattttatc ggtgactgaa acgataccta aaacttctgg
    34201 catcgaatat attgcttttg aggtcttctc tgcgtttggt acagtaggtc ttacgatggg
    34261 attgacgcca gacttgactg caatagggaa attgatcatt atagcgctta tgtacattgg
    34321 acgagttggt gtattgactg tggtcttctc tctgatgaca aaagcacaaa gaacacaatc
    34381 aaaatacaaa tatccagaag aaagtgtcat gattggataa tttcatcttc tgacagaaac
    34441 ctagtgattt agtaaaataa gaaaataaaa taatttttct tgcttcttgg atactttaag
    34501 tgcaaaatgt tcgtttattt tttgaggcac tatccgttgt tgaacctcgg gtagtgcctc
    34561 tttcttattg aaaggaatca ataagatttg attcctttat ttatcaatag gttataattc
    34621 atattttttt attttttctt ttacgatact tagtccagaa gctaaaattt caaggttatt
    34681 agaggatagg gaaattcgaa cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga
    34741 atgataaagt cggataccgt tagcaagaaa gtcattctca attatcttag gatcaatctc
    34801 aggatcaact ggaagaattc gatagtaagg gacacgattc gttggttttt ctactttgaa
    34861 aagagaatca aaaagctgat tggctctttt cgtaagactt aatttagttt ctaagatcct
    34921 atttgcttca tttgaatgga ggatttgagt gatgacttcc acatctaatc cagaagtttt
    34981 cacatttata ttaaaaattg cttgttctat cttgttttgg acatgtactg gaactacaag
    35041 ataagcaact cgcaatccag aacaaataaa ttttgtcatt ccagctaagt agattgtttg
    35101 gttaggtaat aatttttgga aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa
    35161 tgaatggata tcatcttcaa taacccatag accttctttt tcaatcactt cggctaattg
    35221 ctctcgtctt ttgtgagaga tttgaaaccc tataggatta ttacaagaag gcataagaaa
    35281 gacaccatga atcttctttt ttctacattc ctgaatcaat atatcaggtc gcattccttg
    35341 ctcgtcaaaa tcaattggaa cgatctctaa atgatataac tgagccaatt caatgaaatt
    35401 agcgtatgta tatcggtctg ttgctattct atcacctgga gagaaaagtg cagaaagtgt
    35461 gatagccaat ccattttgaa caccggaaac aatagataga gattctaagg acggaatgac
    35521 tccttgagtt tttaaccaat ttgctgcagt agtcaattga aaatttgtgc ctttgggatc
    35581 ttggtaatta agcaaaccaa caagatttgg gttattacta gttgattgaa taaacgggac
    35641 aacgaattta ttacattctt caaaagaact aaccagacca aaatcaatga tatgttcggt
    35701 tacttcattg gtagagatgg tgatggactc aaagacattg ggagaaataa atgtgccact
    35761 tccaatattc gtatacagca aacctttctc aattccatat ttataagcct tgccaacggt
    35821 agtaaaattt aaatctaaat aatcagctag ctctctttgg gagggtaact gagtgttttt
    35881 ctgtaattta ttctttaaaa tatcttgttc taattgttga atcaacgatc gataataagg
    35941 acgcttcaat ttttgtttgt ctggtcgcca agttaacggg taattgtcat aagaattaat
    36001 aggcattttc tacactcctt aaaattgtat accatacaat tttaccattg attgtattga
    36061 ttttagaagt taatcttttg tgaggaggga attgtctatg aaaattatta attttgagga
    36121 agtagtagct tcttttagtt ttgaagaagc gattcaagta atgaaaaaat gtttttttga
    36181 ttatgaaagt ggtaaaatca gccaaaatcc tcgattggtg gaaattttac ctgacggaaa
    36241 agacaaaaat atgtttgcga tgatgcctgc ttatttgggg gagaacagat attttggaac
    36301 aaaagtcatt accgcttttc cggataatca tcagtatcat ttaccttctc atttaggaga
    36361 gattttcttg tttgatgctc aaaacggact acccaaagca ttgataaacg caaatgcagt
    36421 cacctggatt cgaacagctg ctgtatctgc gctggcaaca gattacttag caaatccgca
    36481 tgcagaaaca cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat
    36541 ttctaccatt cgtcccatca ctacagtgtt tgtttacgat attttagaag aaagacgaaa
    36601 tacttttatc gctaaagcta aaaaacagta tcctaaaata acgtttataa atgcaatatc
    36661 agtagaagat gcagtacgtc cagcggaaat catttgtacg ttgacctcta gtaaagaggc
    36721 atttcttcaa aaagagtggg taagggaaga cgcacatatc aatgctgtgg gcacatttac
    36781 tcctgataca agagagataa gtacatcgtt gatggaaact tctcaaatct atgtagatga
    36841 ttactcagct accttaacag aaagcgggga cctgcttata gccatggcag aaggagattt
    36901 agaggaatct tgtattgttg gtacattagg cgaattagtt tctgagaaaa aaacatttta
    36961 taaaggagaa tcgaggccga cgattttcga cgcagttggt ctagctattg aagatctttg
    37021 ttgtgcagaa tatatttata acaaattaga gaaaggggct ttaaaatgaa cttagattat
    37081 tacgtagttg atgcatttgc agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg
    37141 ctagaagaat ggctgccaga aggtacgatg caaaaaattg ccattgaaaa caatttatct
    37201 gaaacagcat ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt tacgccagat
    37261 cgagaaatcg atctttgtgg tcacgcgaca ttagcaacag catttgtttt atttaactat
    37321 tacaaaatac ctgatgaaac gattaaattt tctactcaaa gcggtaatct atttgtgaca
    37381 agaattaaag attattatta catggatttt cctagtatta tgccgaaaaa agtgccgata
    37441 ttgactgaat atgaagaagc aattggggct aagattaaag aagcctactt agcacgtgat
    37501 ttatttttcg tattagaaga tgaaaaaacg gtggctaaat tgcagccaga ttttacagct
    37561 atcaaaaatt ttgaattagg aatagagtca tcgtgacagc agaaagtgtg caaaaagact
    37621 ttgtttctag aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag
    37681 cacattctaa tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt
    37741 atcaaatgtc atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa
    37801 taggaggaaa agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg
    37861 aattaaaaat aaattggtgg agagtggcag ccttatagct acaatggttg ttaaaaaaag
    37921 acgcaaactt tttcaattta atgaaaagtt tgcgtctttt tttaattatt ggtgtttgtt
    37981 ttttattcaa tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta
    38041 taaccatcag ggtcaatgac aaagtagtaa gaaggcgtag tgccaggtaa tccttttaag
    38101 tcagtaatct caagacctgc agctttatgt tgctcgtgta atttttctaa atcatcagct
    38161 gcaatagcga tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa
    38221 gtcaactcta attcatagtc atctccagga agtgtcaaat aaacaagtgt aaatttgttt
    38281 tctggaaaat cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt
    38341 gatgcttcaa gatctttcac ccgaacacat gtgtgagcca ttttcataaa ttcatctctc
    38401 cttttccttt tactcaatca taacaaaaag aagtaagaaa atagttgatt tgtgcttgtt
    38461 catgagaaat ttctcatgta aaatggatag aaaataatac tgttaaggtg gaaacaattc
    38521 atggataaac caatttttgg tgaaaaaaag caatctgcta attatcaaac ccgttatgca
    38581 gcatatattg tcatagaaag agacaagcgg attgcggtga tcgaggctcc aaatggtgct
    38641 tttttccttc ctggtgggga aatcgaagga gaagagacga aagaagaagc gattgtccgt
    38701 gagctgatag aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat
    38761 gagtattttt actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt
    38821 gctgattctt ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg
    38881 agccctcaag aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga
    38941 tggatcgaaa aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt
    39001 tatttattac agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga
    39061 agtgatgaca tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag
    39121 accagcatat tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc
    39181 ttgatcaata ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt
    39241 atttagtact tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt
    39301 ttgcggtaaa cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt
    39361 gacgatgttc atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac
    39421 taaacttgct tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt
    39481 gtcatcgatc gaatggagat gactgccgac acggcgtgcc atcacttcga tctctaggaa
    39541 atcttcatga gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag
    39601 tgccccgtta taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg
    39661 ttcctggaca cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac
    39721 agcagcggca atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt
    39781 accatcgatg tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt
    39841 tatttcaatc aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa
    39901 taaatcataa tgatcacgca aggagggatc aaccatttct tttggaaaga aaaatcgctc
    39961 atctccttgt tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat
    40021 caaagttccg tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg
    40081 ataaaaatca taaggcagat cgtagctaga attcacagca gtgtaataaa cagggttata
    40141 gccaaccttt tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga
    40201 aaaagtagca gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat
    40261 aggatcagca gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt
    40321 gaggtattct tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag
    40381 ttcaaaactg ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa
    40441 aatcacttcc ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg
    40501 gctaacgata taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc
    40561 ataaggacga atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt
    40621 gaaataagca tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat
    40681 ttggacaact tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa
    40741 accttcttct actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc
    40801 tacagtgatg gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc
    40861 atgaccgaca tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca
    40921 gcctccgttg cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct
    40981 tgaaatctca taaacgccta atgaatgggt aaaacggcta tgttctgctc cgtgaaaagt
    41041 gaaagaagaa gtgcctaatt gtttaatgcg tcgtaaacgt tggacttctt tcgaattgat
    41101 caaatctaag attactttat gctggacatg gatatagtta tggactggat cacgaaaaac
    41161 tttttccata ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt
    41221 tattacgttg ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt
    41281 ttagccagtc ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg
    41341 caaatgcttc tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg
    41401 ccattgtagc aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag
    41461 attgataaaa tcgcttcatg acttgcccgc gtttttcctg attaccgttg acacttaagt
    41521 acatcataac agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc
    41581 catggatgct taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta
    41641 tcggtgcctt tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta
    41701 tggttatttt ttttgtgtca ctttgaggga aaatcaaaga aatatttaaa attccttcat
    41761 ctgatacgat ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatctttca
    41821 gtgtttggat cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg
    41881 tgcagtcata ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta
    41941 atatatctgt taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc
    42001 gaatcgcagg gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat
    42061 actctaaaat caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt
    42121 tgacaaaaca aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat
    42181 ggaattatca caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg
    42241 ggaagtgcaa gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct
    42301 gtacatacgt tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa
    42361 attagttcca gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa
    42421 atttggctat aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat
    42481 tgctactttt acgaaagaac tgcatgttag tttgaaagac cgtcccacag cagggaaggt
    42541 tcgaatcgat tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt
    42601 ggattttaca gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat
    42661 aaggagtaga ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa
    42721 caaaagcgaa ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt
    42781 catggatttt tctgatttag ctaaccagat ccaaaactat cttgagaaat cagacagtga
    42841 aattcgtgat tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc
    42901 attaggggat aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga
    42961 agtaaaccat ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa
    43021 agtcaacgcc tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga
    43081 agatacagat ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga
    43141 tgaagaaatc gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga
    43201 tgatgaagaa gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga
    43261 tgatgatgaa gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt
    43321 ttcttacgag aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga
    43381 tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa
    43441 aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga
    43501 atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat
    43561 ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat
    43621 aaagaagagg ccttagatgt gatgcaagat agttttcata aagccttgaa ctattttgaa
    43681 aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca
    43741 ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc
    43801 ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata
    43861 gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa
    43921 attgcaacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg
    43981 aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga
    44041 aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga
    44101 ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa ggcggagaaa
    44161 gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct
    44221 tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat
    44281 tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc
    44341 gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata
    44401 ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac
    44461 ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt
    44521 gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac
    44581 aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag atgacactta
    44641 tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc
    44701 atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact
    44761 tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc
    44821 tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt
    44881 gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga
    44941 ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga
    45001 agagatatag tgaaaagaaa ttatttagtc atcagtatta ttgctttatc cgtgcttttg
    45061 tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct
    45121 tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca
    45181 actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcaa
    45241 tcatcacaat caacacaaga cagtacagaa gcaatccaag acccgactgt caaaagcgtc
    45301 gcaatcagct ttgatgatgg accaggagcg accacaacgc cgcagcttct gcgaatctta
    45361 aaagaaaaaa acgtgcatgt cacttttttc gtcttaggag aaaatacggc gcagcatcct
    45421 gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat
    45481 cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact
    45541 gagatcaaaa aaagtcactg gcaaaacccc cgcttttgtt cgtccgccct atggttctgt
    45601 cacgaatgta ggagcaacga tcatccagcg accgatcatc gaatggtctg ttgattcaga
    45661 agattggaag acaaggaatc ctgatttgat tttgcagaag atccaagcaa cagtatacga
    45721 tgggtcaatt attttattcc atgatatcta tcctgaaacg attcgtgccg ttcctcaagt
    45781 gatcgattat ctgaaagagc aaggctatcg catcacgaca gttagcgatt tattagggca
    45841 tccaacggct gtcgagaact attatggcag aaacgatcat cggccagttc agtaagtgag
    45901 acaagtatct ttaataaata acacatttta ataagtagtt gaacacataa ctaccttact
    45961 tgcaaaggct taacaatatc tatacagata caacaaaaaa ctgtccacca attctctttg
    46021 gcggacagtt ttcacttaaa aataaagaag caactattcg taaattatgc taaaatagca
    46081 gtagaacgcg tcctgaggga atcgaacccc cgtctcaaga accggaatct tacgtgatat
    46141 ccactacact aaggacgcaa gcactgggat aattgtaact aaacagataa aaaattacaa
    46201 ggggattgga caaaaaaatg aaatttgagc aaaatttttc acaaaaacag cagcagacac
    46261 agaaactagc catgacgcaa cagttgcaac agtccatcca agttttacaa tataacagtg
    46321 aggagctgtt ggcattcgtt gagaatcaag caatggaaaa tccattggtg gaagttgttg
    46381 agccggaatg gcagccagat tacataaaag cttcttcatc ttcttatgaa ggagaagaga
    46441 caaattacct aaatcagatt cctgatacaa aagggtcttt atttgattca ttgatcgaac
    46501 aagtccattt gaattaccga gatacttttt tgaggaaaat cgttctttat cttgtagaat
    46561 acatcgattt gaacggattt ttgacgatta gtttagaaga agccggaaaa gagattggag
    46621 ctacgcctat ccaaatgctc gatgcgttga ctctgattca gcaattagaa ccagctggag
    46681 taggtgcacg tagtttgcaa gaatgtctca tgcttcaaac agaacgagat gattatgcgc
    46741 cggaattagc ttatatcgtg ttggaagaat gttttgagga actggtggag cgaaaatgga
    46801 aagaaatcgc acaacgtttt tctgttgatc tacatgctgt tcagcagata ttcgactatt
    46861 tacaaacgtt gagtccaagc cctggccgga ttttcgaccg tagcagcgaa ttgtacatta
    46921 ttcctgatgt ccgggtatta gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa
    46981 aaaatcagcc aaatatacgc tttcaagaga gttacttcaa acaaatgtca caacaagcag
    47041 ataaagaaac tgaaaattat ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa
    47101 cgatcctcca acgtggggat acaattttgc gggttgctca agtcattgtt tcccggcaaa
    47161 aagctttctt tatcgacaag gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa
    47221 ctgagataga tgtgcatgaa tccacggtca gtcgtgcagt caacgggaaa tatttggaaa
    47281 cagatttcgg tgtatttgaa ttaaaaaaat ttttcactac acgaattcct acgaatagta
    47341 cagaacagac agaagacctt tcagcagata cagcgaagaa aaagctgcag gaattagtcg
    47401 atcaagaaga caaaaacaag ccgttgtcgg accagaaatt ggttgaattg ctgaaaaaag
    47461 atgagatagc gatctcaaga cgaacagtag caaaatatcg tgatttatta ggaatcccga
    47521 gttcatcaaa aagaaaacgt tatgataact aatcagtcaa aagactagaa aaagtatttc
    47581 actttttcta gtctttttag ttgaaaaacg agagcaaaac tgttagactt acagtcgtga
    47641 ggttgagatt tccctttatt gatgtgaaat tcgaatagtt aatttctttt tttatcgtat
    47701 atgggtcgtt ttaagtctac attggacgta aagtgtccaa ctaaggagat cgctatgctg
    47761 gatgaaataa aaatgattga atctgttgca ccagatatta tcgaagtgtt gcaagaacgc
    47821 tataaaattt tacgaaatat ctattggatg cagccgattg ggcgtcgaag tttatcggaa
    47881 agtatgggga tcacagaacg agtcttacga acagaaacag accttttgaa aaatctgaat
    47941 ttgatcgaca cctcaaaaag cggtatgacg ctgacaacta agggcgaaga agtttatcaa
    48001 agccttgaaa acttcatgga tcagctttta ggtacgcatc aaaccgaaca acagttggca
    48061 gaatattttg gtaaccaacg ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa
    48121 gtggctgatg cattcggcga agcgctgagc gaagccttag accgactact tccagaaggc
    48181 gagaatatca tcgctgtgat gggaggaact actatggctg tggtagccga acaattgtct
    48241 aatctggaaa ataagaaacg gcataacctg tttgtccctg caagaggcgg gattggtgaa
    48301 gcgataactg tacaggcaaa ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac
    48361 caccgcgcac tttatgttcc ggaacaatta agtctggcaa cttacaattc tttactcaat
    48421 gagccatcta tccaagaagt cttaaacttg attagtgaag cgaattgtgt tattcatagt
    48481 attgggcgtg cattacacat ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg
    48541 ctgaaacaag caaatgctgt tgcggaatct tttggctact ttttcaatga aaaaggtgaa
    48601 gtcgtttata aggtccctag aatcggcata gaattgcaag atttggaaaa agtcccgatc
    48661 atcatggcaa tcgcgggcgg aaaatcaaaa gctaaagcaa ttcgagctta tatgaaaaat
    48721 gcaccaaaac aaacgtggct catcactgat gaagctgctg caaatgagat tttaaaaggg
    48781 gtaacccttt aaaataaata tttttttgat tttcataagg aggaaatctt aaatgacagt
    48841 taaagtaggt attaatggtt ttggacgtat tggacgtcta gcattccgtc gtatccaaga
    48901 tgtagatgga atcgaagtag tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca
    48961 cttgttaaaa tacgacacaa ctcaaggacg tttcaacgga acagttgaag ttcatgaagg
    49021 ttcattcaac gtaaacggaa aagaagttaa agtattagct aaccgcaacc cagaagaatt
    49081 accatggggc gaactaggcg tagatatcgt tctagaatgt actggtttct tcacttctaa
    49141 atcagcagct gaaaaacatt taacagctgg tgctaaacgc gttgttatct ctgctcctgg
    49201 cggaaacgat gttccaacaa tcgtttataa cacaaaccac gaaacattaa ctggtaaaga
    49261 aacagttatc tcaggtgctt catgtactac taactgttta gctcctatgg ctaaaacatt
    49321 gaacgacaaa tttggtgttg ttgaaggatt aatgacaact atccacgctt acacaggtga
    49381 ccaaatgact ctagacggac ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc
    49441 tgcaaacatc gttcctaact caacaggtgc tgctaaagct atcggtttag taattcccga
    49501 attgaacggt aaattagacg gagctgctca acgtgttcct gtaccaacag gttcattaac
    49561 agaattagta acagttcttg aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa
    49621 agaagcttca aacgaatctt acggatacaa cacagacgaa atcgtttcct cagatatcgt
    49681 tggtatgact tacggttcat tatttgatgc tacacaaact aaagtaatga cagttggcga
    49741 caaacaatta gttaaaactg ttgcttggta cgacaacgaa atgtcataca ctgcacaatt
    49801 agtacgtact ttagaatact tcgctaactt ataagataga caaacgtaca agtgtaaatt
    49861 atagacatgt acatttaaaa gcggggaagc aatcgcgctt cttccgcttt ttttgataat
    49921 aagtttatt
//
LOCUS       DAZKCZ010000009        43612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000009 DAZKCZ010000000
VERSION     DAZKCZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.009"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(252..2633)
                     /locus_tag="ACG8KN_000442"
     CDS             complement(252..2633)
                     /locus_tag="ACG8KN_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9187526.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2623..3270)
                     /gene="recU"
                     /locus_tag="ACG8KN_000443"
     CDS             complement(2623..3270)
                     /gene="recU"
                     /locus_tag="ACG8KN_000443"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFP9187527.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3334..3873
                     /locus_tag="ACG8KN_000444"
     CDS             3334..3873
                     /locus_tag="ACG8KN_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFP9187528.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3963..4382
                     /gene="gpsB"
                     /locus_tag="ACG8KN_000445"
     CDS             3963..4382
                     /gene="gpsB"
                     /locus_tag="ACG8KN_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFP9187529.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4404..4771
                     /gene="rnpB"
                     /locus_tag="ACG8KN_000446"
     ncRNA           4404..4771
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8KN_000446"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4980..6146
                     /locus_tag="ACG8KN_000447"
     CDS             4980..6146
                     /locus_tag="ACG8KN_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFP9187530.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6192..7688
                     /locus_tag="ACG8KN_000448"
     CDS             6192..7688
                     /locus_tag="ACG8KN_000448"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFP9187531.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7769..8452
                     /locus_tag="ACG8KN_000449"
     CDS             7769..8452
                     /locus_tag="ACG8KN_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9187532.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9097..10287
                     /locus_tag="ACG8KN_000450"
     CDS             9097..10287
                     /locus_tag="ACG8KN_000450"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9187533.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10389..11342
                     /locus_tag="ACG8KN_000451"
     CDS             10389..11342
                     /locus_tag="ACG8KN_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9187534.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11411..12760
                     /locus_tag="ACG8KN_000452"
     CDS             11411..12760
                     /locus_tag="ACG8KN_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9187535.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12943..13266)
                     /locus_tag="ACG8KN_000453"
     CDS             complement(12943..13266)
                     /locus_tag="ACG8KN_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187536.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13395..14558
                     /locus_tag="ACG8KN_000454"
     CDS             13395..14558
                     /locus_tag="ACG8KN_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9187537.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14628..15977
                     /locus_tag="ACG8KN_000455"
     CDS             14628..15977
                     /locus_tag="ACG8KN_000455"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9187538.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16022..16453)
                     /locus_tag="ACG8KN_000456"
     CDS             complement(16022..16453)
                     /locus_tag="ACG8KN_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9187539.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16841..19483
                     /gene="alaS"
                     /locus_tag="ACG8KN_000457"
     CDS             16841..19483
                     /gene="alaS"
                     /locus_tag="ACG8KN_000457"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9187540.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19683..20279
                     /locus_tag="ACG8KN_000458"
     CDS             19683..20279
                     /locus_tag="ACG8KN_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9187541.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20401..21549
                     /locus_tag="ACG8KN_000459"
     CDS             20401..21549
                     /locus_tag="ACG8KN_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9187542.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21587..22000)
                     /locus_tag="ACG8KN_000460"
     CDS             complement(21587..22000)
                     /locus_tag="ACG8KN_000460"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFP9187543.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22079..22510
                     /locus_tag="ACG8KN_000461"
     CDS             22079..22510
                     /locus_tag="ACG8KN_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFP9187544.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22609..22842)
                     /locus_tag="ACG8KN_000462"
     CDS             complement(22609..22842)
                     /locus_tag="ACG8KN_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9187545.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23025..24695
                     /locus_tag="ACG8KN_000463"
     CDS             23025..24695
                     /locus_tag="ACG8KN_000463"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFP9187546.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24842..25228
                     /locus_tag="ACG8KN_000464"
     CDS             24842..25228
                     /locus_tag="ACG8KN_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187547.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25215..25913
                     /locus_tag="ACG8KN_000465"
     CDS             25215..25913
                     /locus_tag="ACG8KN_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFP9187548.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25918..26382
                     /gene="lspA"
                     /locus_tag="ACG8KN_000466"
     CDS             25918..26382
                     /gene="lspA"
                     /locus_tag="ACG8KN_000466"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9187549.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26379..27290
                     /locus_tag="ACG8KN_000467"
     CDS             26379..27290
                     /locus_tag="ACG8KN_000467"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9187550.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27525..28064
                     /gene="pyrR"
                     /locus_tag="ACG8KN_000468"
     CDS             27525..28064
                     /gene="pyrR"
                     /locus_tag="ACG8KN_000468"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFP9187551.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28078..29361
                     /locus_tag="ACG8KN_000469"
     CDS             28078..29361
                     /locus_tag="ACG8KN_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFP9187552.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29374..30300
                     /locus_tag="ACG8KN_000470"
     CDS             29374..30300
                     /locus_tag="ACG8KN_000470"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFP9187553.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30319..31599
                     /locus_tag="ACG8KN_000471"
     CDS             30319..31599
                     /locus_tag="ACG8KN_000471"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9187554.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31604..32680
                     /locus_tag="ACG8KN_000472"
     CDS             31604..32680
                     /locus_tag="ACG8KN_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFP9187555.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32685..35870
                     /gene="carB"
                     /locus_tag="ACG8KN_000473"
     CDS             32685..35870
                     /gene="carB"
                     /locus_tag="ACG8KN_000473"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9187556.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35924..36700
                     /locus_tag="ACG8KN_000474"
     CDS             35924..36700
                     /locus_tag="ACG8KN_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFP9187557.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36697..37629
                     /locus_tag="ACG8KN_000475"
     CDS             36697..37629
                     /locus_tag="ACG8KN_000475"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFP9187558.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37626..38342
                     /gene="pyrF"
                     /locus_tag="ACG8KN_000476"
     CDS             37626..38342
                     /gene="pyrF"
                     /locus_tag="ACG8KN_000476"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9187559.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38349..38978
                     /gene="pyrE"
                     /locus_tag="ACG8KN_000477"
     CDS             38349..38978
                     /gene="pyrE"
                     /locus_tag="ACG8KN_000477"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9187560.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            38966..39280
                     /locus_tag="ACG8KN_000478"
     CDS             38966..39280
                     /locus_tag="ACG8KN_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187561.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39318..39959
                     /locus_tag="ACG8KN_000479"
     CDS             39318..39959
                     /locus_tag="ACG8KN_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFP9187562.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40002..40694
                     /locus_tag="ACG8KN_000480"
     CDS             40002..40694
                     /locus_tag="ACG8KN_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFP9187563.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40691..41593
                     /locus_tag="ACG8KN_000481"
     CDS             40691..41593
                     /locus_tag="ACG8KN_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9187564.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41751..43457)
                     /gene="efbA"
                     /locus_tag="ACG8KN_000482"
     CDS             complement(41751..43457)
                     /gene="efbA"
                     /locus_tag="ACG8KN_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFP9187565.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13967 a         7691 c         9478 g        12476 t
ORIGIN      
        1 tggcactaaa taaaagacta gtgtgtattt agccgattta attataaaca agtaaatgga
       61 taaagtatat gtaatgataa aagaaaaatg gattttctaa tttttaagct aaatgttaga
      121 tcacacatgt atatgctgga tagtcttata aaaaatcatg taatacagct tggtacatga
      181 tttttatttg tagctgtttt ttacatagac acaaaaaaga aaccacgctt gtagacgtgg
      241 tttctttttt actatctgtt tccagaagct gaggtgctgc tggatggtgt atggtcattt
      301 gcagcatccg ctccgctagt tgctggctca ctggaagaag caggaggagc agatgaagac
      361 ggtgtcgagc tttcttcgga tgaagaagaa ggaaccgttg ttgaagacgc tggctcgctt
      421 gtttctgctg tcgtgctttc ttttgaggac tcagcagttg attcgctttg actagtagag
      481 ctagagcttt gagtcgttgt ctctgttgtt gagcttccag gagtctggac atagctggaa
      541 gatggaatag ttgttgatgg tgtaattgca ttcgatctag ctgtatattg atctttgtag
      601 tacagttcac cgcccacacg aattaatccg cttggcatct cccagtctgt atttgtcacg
      661 ttagctgaaa catactgcat aagctcgcga taaacatcag aggctacgtg tgacgattct
      721 gaagtgacag gtgtcatttt tttattgtaa cctgtccaca cggaaatcga ataatttggc
      781 gtatagcccg caaataggat atctggatac accccagaag aaataccaag ttttgcatac
      841 tcgtcatctg tgtaattgga tgttccggtc ttaccggctt gatataaccc agcgatttgc
      901 gcattcgtac cagttccttc agtgatcgta tcttttaaaa tgtctgtgat catataagct
      961 gtttctggag acatggctgt ttttccatct ggctcataag tttcttctgt tccgtcttgg
     1021 aagacgattt tattaacata ttgaggtttg taataagtcc ctccattggc gaaagccgca
     1081 taggctgctg ccatttttaa ggaagaagct ccgtattttg tcccatcttg ttcttccgta
     1141 ttactagaga tcgcattcga ttgatggatc gtactgtatt cgatcccaag attttttaag
     1201 aaagaagcca ctttatctga accgacttca ttgaataatt tgactgctgg aacattacgg
     1261 gataagtaaa gtgcttggcg taaagtaatg gtgcccatat attgattatc ccagtttcct
     1321 acaggagtcg aagtcccttc atagttatac ggtgcgtcag taatagtttt tccagtagaa
     1381 tatttcaaat attcaaaagc tggtccgtaa tctgtgacag gtttcatcgt agaaccaaaa
     1441 tcacgggaag tattcactgc taagttgttg cctaaagtta cgtcttctgc gatgtgacgt
     1501 ccgccaatct gggcttttac tttcccggta ttggtgtcga taagggtgga ggcaacttgc
     1561 atctcgtcgt ctgggtaaga gacatactga tccgtgttga cgatatcata tagtttcttt
     1621 tgggcgtcca gatccaaatt cgtataaatc tctaaaccgt ctgtgtagac attcttatct
     1681 gttttttctt gtacttcatt gatgacttct ttgacataat tatcgacgat cttagtattg
     1741 tcatcggatt gagttagttc ttgcagacca tccgttaccg gtacattaac agcttgatca
     1801 tattctgttt gagagatttt ttcgttttga agcatcgtat acaaaacagt atctcgtctt
     1861 ttcttcgcct gatctggata aacataagga tcataggcgg aaggggcttg aggcatccca
     1921 gcaagcaatg ctgtttgcgg taaagaaagt tctgataatt tcttaccaaa gtacatctca
     1981 gaagctgtct ccatcccgta caagccgttt gacatataca ctttatttac gtagtaggtc
     2041 aagatctctt gtttcgattt tttttgttcc aatcttacag ccatccacgc ttcttgggcc
     2101 ttgcgtttca gagtctgatc ctcggcactg gtggagaaga aggaaagttt gatcaattgc
     2161 tgtgtcaaag tacttccgcc ttgcagtccg ccagaggtaa agttagagag ggcagaaccg
     2221 atgatccgaa tagggtcaac cccgatgtgt ttataaaaac ggcgatcttc taccgacacg
     2281 atcgcatctt ctaatgtttt cggtaattca ttcgcagaaa ttttttcacg cttttctgcg
     2341 cctaaatctt cgaatagttc gccatcttgt gtatatagtt tggaagatac ggtagcgtct
     2401 aattttttat ccgtcaattc gggcgcatct tttgcgtaat accagaaaag tcctacacct
     2461 gctaaaaaga ggatacagaa aaatgagagt attcctaaaa ggatcttgat aaataatcct
     2521 tttttatgtg ttccagagga ctttccagaa cctttgccag aattcttctt cacactcttt
     2581 ttaggagtag gctgtttctg tcgtctagag cttctagttt gttcatttgc cattcttgct
     2641 ttctccttca atagataatg ttgataaata gaagtcaacc gcgtccaaat aaggaatccg
     2701 cggagaaata ccagttttga tctctattcc tagttcttcg atcaaagata aaggtagtga
     2761 tttgcgtcct ttttcctttt gggcgtccca gtaagcaatc aaatgaacac tattcaaata
     2821 aaaacagcgc ttcaaagacg aaaaccataa caagacaaag cagaccccat tttgttcgag
     2881 gcatgctttc atatgttcga tttgatgggc atggaagttt ttaaagggaa aagatgtttt
     2941 gttcttggtt tcttttgctt caaagtccaa gtaaaagcct cgatagacgc cattatagtc
     3001 tgtagttgag gcttgggtaa agtaggcttc cttgatcaca gcagcacttc tcttaggata
     3061 gtctactttc acgatttgga taggggtagg ctttttatgg ataaccgcca acccgcggga
     3121 aagataatat ttattacttt cattgattgc ttcttcaaag cgcatccccc gattgccgaa
     3181 ttcagtgatc tgatttcttt tttcttgaat tttttcgacg ggagaggcat tcttataata
     3241 aggttggcca tttggatagc gcaataccat ctttaatcac actcctaggc cacattatag
     3301 caaaaaagcg gatagaaaga aaaggaatct gagatggata agtcaaaagt aatctatgtg
     3361 tctgggtatc gaagtttcga actcggtgtt tttaaagaaa atgatcctaa aatccaagtt
     3421 gttaaaaatg ttttaaaaaa agaactccat cagttagcag aagaagggct ggaatgggta
     3481 cttgtcagtg ggaatttggg cgtcgaacta tgggctgtgc aaatagtagc ggaattgaaa
     3541 gaagaatatc cggaactatc tcttggtatt ttgtatcctt tttctgattt tggtaataac
     3601 tggaatgaag ggaaccaaga aaaaaaacaa ttagcagaac aattagcaga ttatgtcgaa
     3661 gctgttagcc atcaacccta ccaatcacct agtcagctaa agaatcacac tcgttttata
     3721 cttgagcata cagtgggctg tctgctgatc tatgatgaag aattccctgg aaaaacgaag
     3781 tatttttttg aagatgcaca gaattaccaa gcagatcatc cctacgaaat tcggctgatc
     3841 agtatggacg atcttcaaaa ttttagtgaa taatcgatat tgtgtttgat ttatcaactt
     3901 ttttcctata caatgaaaaa ggtagaaatg ttattacgta aatgaaaact agaggtgtaa
     3961 atatggcgaa tttggtttat agtcctaaag acattttaca acaagaattc aaaacaaaaa
     4021 tgcgcggcta tgatcccgtt gaagttgatg aattcttaga caatatcatc aaagattatg
     4081 aaacttacag caaagaactc cttgctttac aagaagaaaa cgatcgattg agtgcaaaag
     4141 tagctcaatt atctaaaact caaggagcag ctcaaacacg cgtgcaacaa acagaggcac
     4201 caaagagcgc agcagtgacc aattttgata tcttaaaacg cttgtcaaat cttgaacgtg
     4261 aagtatttgg taaaaagctt gatcaacaag caagtgcagt caaaccagca caacctaatc
     4321 caaataacta cacaaatgca gacacaagct tagatgataa tgaaaaaaca cgtcaatttt
     4381 aatttttgat taatgaattt gcggttttcg ggtaatcgcg gtctgacttg atcaggctga
     4441 ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt tcgtgcttag cgaaaccata
     4501 agctaaggta ctctttggag taacggcagg aaaacaagct aaggcttcgg ctatgcttaa
     4561 gtatccttga aagtgccaca gtgacgaagc ggctggggaa acccatccgg tggaacgcgg
     4621 taaacccctc gagcgagcaa cccaaacaat ggtaggggcg ctttttgtct ggaagaagaa
     4681 caggacaaaa aggcagtata actgcagata gatggttacc gctttgtttt ttctccctgg
     4741 aaaagcaaag tacagaacat ggcttataga aaaccgcaaa catcagggaa ttaggtcgtg
     4801 acaaaagtaa tgcgttataa gaaacaagaa aaacatttga aagctatttt tcaatacttc
     4861 agatgaatcg ttcttattac aaaacaaact tttgtatcac cggttattcg gaatttgagt
     4921 aaaagtgtgg ggtaggactg atgtcccacc tccttttttt ataaataagg agattgtgta
     4981 tggaaaaaaa caggaagttc aacctcgtcg caactgcggc aagcgggcta gaagcactgg
     5041 ttggtaaaga attaagagat cttggaatcg aatgccaagt tgagaacgga cgtgcacgtt
     5101 ttgaaggaac aatggaaacg atcgctacag ctaatttatg gttacgtaca gcagatcgag
     5161 taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga tgaattgttt gaaaacgtaa
     5221 aagcattgcc ttgggaagat tttcttccac tagatgcagc ttttcctgta gctggaaaat
     5281 cgatcaagtc gaaactatat agcacacctg attgccaagc gatcacaaaa aaagcaatcg
     5341 ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt accactgacg gaaactggcg
     5401 cacattttca gttggaagta gctttgctga aagaccatgt gatggtaact ctggatacga
     5461 caggaccaag tttattcaaa cgtggttatc gcttagaaaa aggtggagca ccattgaaag
     5521 aaaacatggc agcagcactt attatgttga caaattggcg aaaagaccgt ccattttatg
     5581 atcctgtatg tggttcaggt acgctttgta ttgaagcagc cttaataggc cataatatcg
     5641 caccaggatt caatcgtgag tttgcttgcg aatcttggga ttggttcagt caagaaatca
     5701 tggaaaatgt tcgatcagca gccgaagaaa aagcagatta tgatattgaa ttagatatta
     5761 ctggttcaga tatcaatgga cggatgattg aaattgccaa agccaatgca gaagaaatcg
     5821 gattaggctc ttcgattacc tttaagcaac aagcagtcaa agacttcaaa acggataaag
     5881 aatacggggt catcgtggct aatccgcctt acggagaacg tctaggagaa gaagaaactg
     5941 ttcggaaatt atataaagaa atgggcgatg tattccgtcc attaaaaaca tggagtaaat
     6001 atatcttgac tagcgactta gcgtttgagg aattttacgg acaaaaagca accaaaaaaa
     6061 gaaaattgta caatggtgca ttgcgtacgg atctattcca gtattgggga actcgtccgc
     6121 ctagaaaaaa agagaaagac gaataatctt actaattgat tcattcaatt ggatgtttgg
     6181 aggaataggg aatgaaagaa aaagagtttt tacaagaagt aaaagaaatc aatcttctac
     6241 aacaagcttt aggaatcctc gattgggata cacaaacagg tatgcctgaa aaagctagtg
     6301 cataccgaag tgaagtagac agctatcttt acagcattta tttttcaaaa aaaatcggtc
     6361 caaaaatcaa agaagccatt cgctattttt ctgaacatcc ggaagaattg tcggaagttg
     6421 gaactgccgt ttttgccaaa gtaaaagaag agtatgattt ggaacatcgc gtaccggaag
     6481 atcttatgag aaaatataca gctgccgttt cttcagcaca cggacaatgg caaaaagcta
     6541 gagaaacaca acaattttct gattttcaac acgcattgaa tcaaaatatt gaattaacaa
     6601 aacagttgat accttactgg agaaaagatg aaaagacgaa ttatgatgtt ttgctgaatc
     6661 aatacgaacc cgggatgact gtcgaaatcc ttgataaggt atttgatcag cttaaagaag
     6721 gaattctttc gatccgccgt acgctccaag aaaaagggac tgaacctgaa acagactttt
     6781 taaatcggag aatgacgaaa aaacaacaga agagatttgt agtgaaagta atcgaacaac
     6841 ttggttatga ttttagtcgt ggtcgtttag atgacactgt ccacccattt atgactggaa
     6901 ttaatccgaa tgatgcacga atcacgacac gatggaacga aacagatttc aaaatggctg
     6961 tttttggagt gatccacgag gctggtcatg gtatgtatga acaagacatc gatgataaat
     7021 atgcgtatac accggtttat gaaggtacat caatgggtat ccatgaatca caatcgttgt
     7081 ttaacgagat cattattgga agtagtcgca gcttctggaa aaagcaatat cctttctttc
     7141 aagaatgtgc agaaggaaca ttcgatgata ttccttttga caaattttat cgttcattga
     7201 accatacaaa atcaagtttg gttcgtatcg aggcagacag tttgacttac cctctccata
     7261 tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga aggggtagat gttgctgaac
     7321 tacctgaaat atggaatcaa aaatatgaag agtatctggg aatacgacca acaaatgatg
     7381 tggaaggcat cctacaagac gttcattggt caggtgcaag ctttggttat tttccctctt
     7441 atgcattagg gtacatgtat gcggcacaat tacttcattc tttgcagcaa gaaatagatg
     7501 tggaaaatgt tctagcgtca gacgattatt ctccaatcaa aaactggatg tcagagcata
     7561 tccatcaata tggtgcatct agaaaaccaa atcaattaat catggatgca acacatgaac
     7621 cgcttaatcc gcaatacctg atcgactata tgaaagacct gtattttaat gtatatcagg
     7681 taaaataaaa tacaatttac aaagatagct gaaattcatc taatctatag atgttttcag
     7741 ctatttactt aagaaagaag tggaacgaat ggataaatta caagtgatcg ctgattatag
     7801 tatggaccaa ttagcgcatg atcaaacagg acatgggagc gatcatacca aaagagttgt
     7861 caagttagct gaaaaaattt tggagacgga accgcaggca gaccgctttg tgacgcttgc
     7921 agcagcttac ctacatgata cgattgatga taaagtcgtt aaaaatgaga acgaggcaaa
     7981 gcaacagctt cgtgtattcc ttcgctcttt gccaatagta gaggaacaga tctcgatgat
     8041 tttttccatt atcgaaaata tgtccttttc taaaaatctc tctgaagcgg ccgaactatc
     8101 tctggaaggt aagatcgttc aagacgctga tcgtatcgaa gcattaggag caatcggcat
     8161 tttgcgtact gcttatttcg gcggtggaca tgggcacccg atttttgatc ctgaactgta
     8221 tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc acgacagtga tcaatcattt
     8281 ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact gattacggat atgaagaagc
     8341 caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa ttttttctgg aatggtctat
     8401 tgacgatgaa aattttactc cagacaattg gatcaaaaat aacaaaaaat gatggaggtt
     8461 gtgacagaag tggtcagctc caagatattt cggatcattt tccgtaaaag ttacttctgg
     8521 aacactgttt accaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt
     8581 tcaaaaaata gcttctcgtg tttgatgaat attgtaactt gtgattcgga attggcagga
     8641 gaacaccgtt tattcggaag aataggactg tgacaagact tttgtcacag tccttttttc
     8701 gaattcaaca agtaaaactt ttaagaaata ttcagagagt aaaattaaaa attgctgata
     8761 aacgaagatg gaaattttat gtatttttat tggtctatgc aataaataaa ttaaatattc
     8821 agaatacaat tgattttaac gaaagggctt ctatcaatgg ttgtgttgta tttatctgcc
     8881 caggtagagt aaaatagatc attatctaaa aggtaattgt tcatactata taagtcattt
     8941 gaaagtaaaa agatactgta aataaaaaat gtggttaaat aattctcata atcagtgtaa
     9001 gcgttatcaa aaatatagca tacttgcttt gtagatgaat gacacgatat atgtttggta
     9061 tgttctgcaa gacagatgaa tatggaggtg gagacaatgt cttccaaaga actaacaatg
     9121 tatttagaga aagaattgga tcaactgaaa gaaaaggggt tatacaatac gatcgacata
     9181 ctagaaagtg aaaatggtgc ttgtatcatc gtagacggaa aaaagatgat caatctagca
     9241 tccaacaatt atcttggatt tgccaatcgc gaagaattga aaaaagcatg tattgaagca
     9301 accgaaactt atggtgtagg agcaggagca gtccgaacga tcaatggttc gctgaaaatc
     9361 catcagcaat tagaagaaaa aatcgcagaa ttcaaaggga cagaagcagc aatcgccttt
     9421 caatctggat tcaactgcaa tatgggcgct atctctgcag taatgacaaa agaagatgct
     9481 atcttatcgg atgaattaaa ccatgcatct attattgacg gttgccgctt atccggagca
     9541 aaaatcatcc gaattaaaca tcaagatatg aaagaccttg agaaaaaagc aaaggaagcg
     9601 atagaaagta agaaatataa aaaaatcatg tatatcacag atggtgtttt ctccatggat
     9661 ggagacattg cacgattgcc ggaaattatt ccgattgttg aaaaatatgg actgatcacc
     9721 tacgtcgatg atgcgcacgg ctcaggcgta actggaaaag gtgcaggaac cgtcaagcat
     9781 tttggattaa gcgacaaaat cgatatgcaa atgggcacgt tatcgaaagc tataggtgtt
     9841 gttgggggtt atgtagctgg atcgaaaaca ctaatcgact ggttgaaagc tagaagtcgg
     9901 ccattcctat tttctacttc gctaactcca ggtgcagctg cagctgcatt ggcttcgatt
     9961 actttgatgc aagaacatcc ggaacttgtc gaaaaagttt gggaaaatgc taattatttt
    10021 aaagaagaat tgaaaaaagt aggctataac atcggtatgt ctgaaacacc tattactcca
    10081 gtcattttgg gagacgaaaa agtgactcag actttctcta agaagctgat cgaacatggg
    10141 atttatgcta aaccaatcgt ttatccaact gtgcctcttg ggacaggcag gatcagaaat
    10201 atgccgactg cggaacatac gagggaaatg cttgatgaag cagtagcagt ttatcaaaaa
    10261 ataggaaaag aaatggaaat tatttaacta aatttagttt tttcgtctat ttaaaattag
    10321 agaacatgtt cacgcatata ttgttaatca tctgaagata gtagtaaaaa agatcaggga
    10381 ggatcatcat gaaaaaagta ttggtgacag gatgtttagg acaaattggt tcagagttga
    10441 tcacacggct acgtcaagaa ctaggcagtg aaaatgtcat tgcgacagat atacgcatgc
    10501 ctgaagtcat tgagaaaaac gatatctttg agatcctaga tgtaatggat tacgaaaaaa
    10561 tgcgcagcat agcagaacaa tatcaagtgg acacattgat tcatttggct gctcttttgt
    10621 cagctgttgc agaagcaaaa ccacaattag cctggaattt gaacgtgact gggctggtca
    10681 atgcattgga agtcgctaga gaattccagc tgaaattttt tacgccaagt tcaatcggcg
    10741 cgtttggtcc aaatactcca aaagatcata ccccacaaga tactcttcag cgtcctacaa
    10801 ccatgtatgg ggtaacgaag gtagctggag aactactatg tgattattat tatgtgaaat
    10861 atggcgtaga tactcgtggt gtccgtttcc ctggattgat ttcttatgca accttgccag
    10921 gaggcggtac gacagactac gcagtcgata tttattattc agcaatcaaa gaaggaaagt
    10981 acactagttt cattgatcaa ggtacatcaa tggatatgat gtatatgcca gatgcgatcg
    11041 atgccattat aaaactcatg aatgcagatc ctgatcgatt ggttcatcga aatgccttca
    11101 atatatcggc gatgtctttt gacccggaag agatcaaagc atctattcaa aaaatcatac
    11161 cagattttga aatggattat gatgtggatc ctgtacgtca gtcaatagca gaatcttggc
    11221 caaattcact agatgtttct tgtgcgcaag aggaatgggg cttttcgccg aaatacgact
    11281 tagatgtgat gacaaaggat atgttggaaa aactgaaaga aaaattggta gagacggtat
    11341 aaaacaagaa ccacatccaa aaagtttttt atacttttgg gattggctat ggtatgaaag
    11401 gggacaaggg atggaagaat atcaagccac gccagtaaaa gaagtaaaaa tagaaaatga
    11461 attaaaacgt acgatgggat tttttactgc tttatcgact gttatgggca cagtgatcgg
    11521 tgcaggggta ttttttaaag ccgcaagcgt agcagaagtt acaggatccg ccagtttgca
    11581 tatgttttct tggtttctcg gtggtatgat ttctgtctgt gcaggactaa caggagctga
    11641 actggcagct gctattccag aaacaggagg gatgattaaa tatatcgaac ggatttacgg
    11701 aaatacagca gcttttttat tgggctgggc gcaagtcgtg atttattttc cagcaaatgt
    11761 agcagccttg tcgatcattt tcggtacaca gttcgtcaat ctatttggtc tttcccagtc
    11821 aatgattgtt cccgtagctg ttacagctgc tgtatcgatc ctgttgatca attttttagg
    11881 ttctaaagct ggaggggctt ttcagtcgat tacgcttgtc tgcaagctta ttcctttgtt
    11941 tgtgatcgtc attttcggtt tgttccgaca agaaggagta gacttccaac tgtttccgat
    12001 ccaagcagga gaaaatcttt catttttctc agccttggga gcgggcttgc tagcaacgat
    12061 gttcgcatac gacggttgga tacacgtagg aaatatttcg ggagaattga aaaagccggc
    12121 gaaagatttg ccaaaagcaa tttctttagg aatcatcgga atcatgatcg tttatttatt
    12181 agtaaatgcg gtgttcttaa gaaccgcatc tatcgacgga gtcgcaggaa acagcaatgc
    12241 ggcaagtgac gttgcaaaaa tgatttttgg cggcttcggt ggaagactgg tcacagttgg
    12301 tatcttgatt tctgtttatg gaaccatcaa tggctatacc cttactggaa tgcgtctccc
    12361 ttatgttatt gcaaaagaaa acaacctgcc ttttagcaag ctctttgcta aacttcatga
    12421 taaaactaaa gttcctgtag ccgcaggtat cttagagcta gtcatcgcta ttggcatgat
    12481 gatgatcggt ggttttgata cactaacgga tatgctgatc tttgtcattt ggatttttta
    12541 tacgatggtc tttgttggtg tcattcttct tcgtaaaaaa gaacctgatt tgtttcgacc
    12601 atacaaagtt ccaatgtatc cgtttattcc actagtagca atcattggag ggacttttat
    12661 cgtatcaagc acgcttatca ctcagacctt tttagcttcg atggggatag tattgacact
    12721 tgctggtatc ccgatttatc tctatttaaa acgtagatag ctaaaacatt cgaattttat
    12781 tagaactgca cttctctgcc aaacaaaata ttttatttaa atgaggagta cgtggaagaa
    12841 atgtcaaaag tactcgttaa ttttgatcac gtaaatgtcg ttttgtctac gatgaaatag
    12901 caaagtaaaa gacagaaatc gggctcaacc gatttctgtc ttttactttg atttgcgtaa
    12961 ctgaactatt cggcgtacgc cgcgataaat atttaataaa accaagcaac cagaaatgaa
    13021 aatgggaagc caaatcagat agacagggta gccaagcact ttagacaaag catgtggagt
    13081 cattttatcc atttcaagaa gtgcttgctg ccaagtctga agattgaaga ctagcatgaa
    13141 acccgaaata atcaggagaa taaatcctaa ataatgaatt gtagttcttt ttcttacata
    13201 agcgatcgta gcataaagga acagtaaaaa aataggtacg ccaaaaacaa agtacatatt
    13261 cagcatctcg acaccccctt tgtgtttatc ttactttact ttgaaagaaa tgagaaataa
    13321 tagcctcgtt acttgcttga aacagcaaag gactgctaag ataagagaga tacaaagaat
    13381 caaggaggag tcatatggca agttatatcg aaccgattcg ttttgcgctg attgtttttc
    13441 catttttagc cttagcgatc tcatctgttt tttttattta tgaatatcga aagtatggaa
    13501 catttctagt aacaagggca gttattttgt attctttcgt tttttattta ttgtgtgcgt
    13561 attttttggt gatcttacca cttccggcaa aggagacggt tgcccaaatg acagggccaa
    13621 agatggagct gcatttattt gcttcatggc aacatttcag agacaacacg gtattggtct
    13681 tgtctgaacc tagtacctac ctgccggcaa tgaagcagag tgtatttttg gaaccagctt
    13741 ttaatgtttt attactcata ccatttggca tttatttaag atattatttc cggctttctt
    13801 ttatgaaaac gatgattgct agtttctgcc tttctctatt ttttgaattg acgcagctaa
    13861 gtggacttta ttttatctac ccaagaccat accgcttatt tgatgtgaat gatttagtga
    13921 caaatacatt gggtggaatc attggttttg tcgttacgcc gttatttaca tttatgcttc
    13981 ctacaagaag acgaatggat gaactttctt atgaaaaagg aaagactgtc tcattgactc
    14041 gacgcttggt cgcttatttg atcgactgga catttttatc tcttattaat ttggtagcgg
    14101 cgattttctt taaaattgcg ttcgattcag gggcagttac ttctcatatc tggtggcttt
    14161 tattagaagt aggggtatac tttatgattg taccgtttat aaccaatgga tggacacttg
    14221 gcaaaaaaat tgtgcgtatc cgtttggtgg aagaagaccg cgaacgaatt tctttaaaag
    14281 cattgatgat ccgctacggg tatctgtact ttctttttta tggtttaagc tggttggggc
    14341 agttgcttca gcaaatcatg aattcgacaa cgaatacggt gttgcagatg agtgcagtat
    14401 tcatttctct cggtgtcttg ttactacaag gtatttttat tttaaatatc ttgctatcat
    14461 ttttgagaaa aaaacggatt ctgttttatg aaaaagctag tcacacatat actgttagca
    14521 cgattggcca aacaaaggat gatgattctc gaaattagct gagcaaaaac gctttgtctg
    14581 tgttataatg ccaaagtcgt gataatttaa agagaagagg tgcacgaatg aataattttg
    14641 atatattccg cttcaaaccg tttatcaatg aagcgttaac cgcaaagggc ttcaaacagc
    14701 ccacagaggt ccaggaacgt ttgattcctt taattgcaaa aggaaaaaat atcattgggc
    14761 aatctcaaac tggtacagga aaaacacata cgtttttgtt gcctttgttt gaaaaaatca
    14821 atgcagaaaa agaggaagta caagttgtta ttacagctcc aagtagggaa ctagctgcac
    14881 agatctatga agcagctaca caaatcgcta aattttctac accggagatc cgtgtctcga
    14941 attttgtcgg cggtacagac aaacagcgtc aattaaatcg tctgcatcac caacagccac
    15001 aggttgtagt aggcacgcct ggtagaatat tagacatgat gaatgaacaa gcgctgaaga
    15061 ttcacaccgc atttgcattt gttgtcgatg aagcggatat gacattggat atgggttttt
    15121 taaatgaagt agaccaaatt gcaagtcgat taccagaaaa gctgcagttt ttagtttttt
    15181 ctgccactat tccagaaaaa cttcgtccat tcttacgaaa atacatggaa aatccaatca
    15241 ttgaagaaat caaaccaaaa gcagttattt ctgaagacat tgaaaattgg ctgatttcaa
    15301 caaaaggtaa agatgagaat caaatgattt atcaactgct gacaattggt catccttatt
    15361 tagcaatcgt atttgccaat acaaaacagc atgtggatga aattgccgat tacttgaaaa
    15421 accaaggctt gaaagtagca aagattcatg gagacatcac cccaagagag agaaaacgag
    15481 tgatgcgaca agtacagaat ctggagtatc aatatgttgt agcgactgat ttagctgcta
    15541 gagggatcga tatcgaaggt gtctctcatg ttattaatgc agaaattcca gaagatctag
    15601 acttctttat ccatcgtgtc ggacgtactg gacgtaatgg cttaaaagga acggccatta
    15661 cgttatattc gccaaatgat gagcaagcca ttgatgcgat agaaaaattg ggcgtctcat
    15721 ttgaaccaaa agaaatcaaa aacggggaaa tcgtcaaaac atacgatcgt aatagacgtg
    15781 ccaaacgaga aaaatcaaaa gatgtattag atcccacgct gattggtctt gtgaagaaga
    15841 aaaagaaaaa gatcaagcct ggttataaga aaaaaatcga ttgggcaatt gcagaaaaaa
    15901 ataaaaaaga acgcaaaatc gaacgcagac agcaaagccg tactgctcgt aaaaataaga
    15961 aaaattcaca taaataaaaa acaagaggtt gtgacaaaag gcgtgtcaca atctcttgtt
    16021 tttaagctag actttttttc attccgatac aaagtacttc atcttcaata aaaggttctg
    16081 tgcaagtatg atacccttgt ttttcataaa aatctacagc cgtcatttca gctactacat
    16141 aactagtctg acaaccttct gaaaccgccc gtttttcagc agcttcaagc aactgaccgc
    16201 ctaacccttt tcctcggtat tcttttgcta cgcaaaatcg ttccggcttg aagcaagtag
    16261 gtgttttctt ttcataacgc aaagtagcta ctggtctttt ttgatcaaga aatagataat
    16321 aggtttgttg cgggatatct tttccgtcga attctagttc tgcaggaatc ccttgttcta
    16381 atacaaaaac ttgatatctc aaaaagcagc tagctgcgcg tatccattca tccgctccaa
    16441 ctattttttt catcatattc ctccttgtca tgaatagcag agatgtattg attcctttat
    16501 tcttatcgta ccaaaaatcg tggagtatga ccaaaaggaa atgaaaataa aaattgagat
    16561 attgacagtg atcaatagaa tgcgtatact aattaagtac gaaaaggata tgatttgttt
    16621 tttaagactc acagaaaatt ctttcttggc tgaaaaaaga atagtcacta atcaaattgc
    16681 tccctttttc tattttggta gaaataccga cgcagctcag cgttatcgag tttaagaggt
    16741 aatgattaca catcattgca atcaaggtgg taccgcgagt tttcgtcctt ggctgtgatg
    16801 gtgttttttt gtatttaatt tatagaaagc aggtaatgac atgaaacaat taagtagtgc
    16861 acaagttcga caaatgtttc ttgatttctt ccaatctaaa ggacatacga ttgaacctag
    16921 tgcgtctttg atccccgtga acgatccaac cttattgtgg atcaactctg gtgttgcaac
    16981 attgaaaaaa tattttgatg gttcagttgt gccagaaaat ccaagaatca caaatgccca
    17041 aaaatctatt cgaacaaatg atatcgaaaa tgtcggtaaa acagcacggc accatacgat
    17101 gtttgagatg ctgggtaatt tttcgattgg tgactatttc aaaaatgaag ccatccactg
    17161 ggcatgggaa tttttgactg atccaaaatg gatggcattc gatccagaaa aactttatgt
    17221 aacagtttat ccaaaagata cagaagcaaa acgcatctgg catgaagaag ttggcctgcc
    17281 actagatcac attattgatg tagaagacaa cttctgggat atcggtgcag gcccatgtgg
    17341 tccggatacg gaaattttct atgaccgtgg agaagcttat aacgatttag cagaagacga
    17401 tcctgaaaac tatccaggag gagaaaatga acgttattta gaaatctgga acttagtatt
    17461 ttcagaattc aaccataagc ctgataacac atttgaacca cttcctcata aaaacattga
    17521 tacggggatg ggattggaac gcatggtctc aatcgttcaa gatgcgtcaa cgaactttga
    17581 gacagacttg tttatgccta ttatccacgc cgtagaaaag ctaagcaaac aagtaaaata
    17641 tggtgaaaac ccagtaacag atatttctta caaagtaatc gcagatcata ttcgcgccct
    17701 ttcatttgct attggcgacg gtgcattgcc atcgaatgaa gggagagggt atgtcttacg
    17761 ccgtttgctt cgtcgtgctg tcatgcatgg gaaaaaatta ggtattgaac aagcattctt
    17821 atatcgttta gtccctgttg ttggggaaat catggtaagt tattatcctg aagtacttca
    17881 acaaaaagaa tttattgaaa aagttatccg tacagaagaa gaacgtttca atgaaacaat
    17941 caatgaaggt ttggaaatct taaatcaagt gatccaagaa gtaaaaaacg aaaacgggac
    18001 gactctagca ggaaaagaca tcttcaaact atacgacaca tatggcttcc cggtagaatt
    18061 aacggaagag gtggcagaag atgaaggact gaaagttgat catgctggtt ttgaaaaaga
    18121 aatggatgca caacgcgaac gtgctcgctc tgcccgcagt actgaaaaat caatgggggt
    18181 acaatctgct ttattgacag atatcaaagt agaaagtaaa ttcattggat acacagaaca
    18241 agaatcagat agtcaattat tggtcatcgt taaaggtgag tctcttttga atgaagtcgc
    18301 agaaggtgaa gctcaaatga tctttgatca aacacctttc tatgcagaaa tgggtgggca
    18361 agtagcggat caaggatgga tcatgaatga agtaggcgaa atcgtagcag ttgttacaga
    18421 tgttcaaaaa gcgcctaatg gtcaatttat gcacaccgta gaagtaaaag caccaatgag
    18481 tgaaggacaa acctatcatc tattgatcga tcaatcgatg cgcaatcgga tcatcaaaaa
    18541 ccatacagca acccatctac tgcacaaggc attgaaagaa gtacttggcg agcatgcaaa
    18601 tcaagctggt tctttagtcg ctcctggaca tttacgattc gactttaccc actttggtca
    18661 aatcacttca gatgaattag ctgaaatgga aagaatcgtc aacgaaaaaa tctgggaagc
    18721 tatcccagtt gaaacaattg aaacagatat cgatacagcc aaaaacatgg gtgcaatggc
    18781 tttgtttggt gaaaaatacg gacatgacgt tcgagtagta aatattggcg gctggtcaat
    18841 cgaactgtgc ggaggcacac atgtagcaaa tacagaaaac atcggaatct tcaaaatcgt
    18901 ttctgaatca ggaattggtg caggcgtgcg acgaatcgag gctgtaacaa gtaaagaagc
    18961 gtacgaacta cttgaagaag aagaaaaaca actaaaacaa gtagcaacaa ttgttaaatc
    19021 acctcaattg aaagaagttg tttcaaaagc ggaacaattg caacagcaag taagagagct
    19081 acaaaaggaa aatgaacaat tagctagcaa gttagcgaat caacaagctg gtgatgtatt
    19141 caaagatcca gaaacgatca atggaaaaac aatcattgca gcacaagtaa aagtgaaaga
    19201 tatgaatcag ctgcgtcaat tagctgacca atggaaacaa aaaaatattt cagatattct
    19261 tgttttagca aacgaacaag aaggaaaagt cagtctgcta gcggcaatga cgaaagaatc
    19321 aaatgaagct ggactaaaag ctggcgactt gattaaagca atcgcaccga aagtaggcgg
    19381 tggcggtggt ggacgtccag acatggcaca agctggcggg aaaaatcctg ctggtattcc
    19441 agatgcactt aaagctgcga aagaatgggt tgcgcaacaa tagggcgttt acgtttgaca
    19501 gaaaaacaaa agttcaaaca ttgaacgaaa catgattatg ggggcgaaga caaacaagtc
    19561 ttgccctcat acttttttca attggatggc gcatctaatc aaatggtcgg attcggatgg
    19621 tctttagttg acgaaagtga cagaaatttt tacgattagt ataaataaaa aggggtgaaa
    19681 aaatgaatag tagaaataag acctttcgac tagtcttacg tgcgatcttg ttagcaatca
    19741 tcatcgtaca agcgatggtt ccttggctgg ggtttattcc attaggattc attagtttaa
    19801 caattatcca tatcacagta attattgctg cagtagtatt aggcccaaaa gatggcatgg
    19861 tgattggctt gttttgggga atcgctacaa ttgttcgtgc ctatgccatg ccaacaacgc
    19921 catttgatac attagtgttc acgaatccga ttatctcggt agttcccaga gtacttgttg
    19981 gcttagtagc aggcttggtt ttccactgga tttatcaaaa aaataaatca ataacgatca
    20041 gctcagcctt tgcaggggta ctgggttcat tggtcaatac agtcttagtg ttaggattca
    20101 tgggattatt ttatacaggt gcaacggcag atgcctatgg tgtagactct gctcttttat
    20161 ttaaaacctt agcaggggtt gcagcaatca atgggatacc agaagcaata ggtgcgggaa
    20221 tcataacacc attactagca aaagcgttat ttgcagcaac accgttgaaa ccagaataat
    20281 gaccatcttt taatagtaat aggacgcacg atagatagaa aaaggaagag aggagaggaa
    20341 aacaatcgtc ctctccatct ttttacctga gcaacaagca aactagaagg ggtaaaaaac
    20401 ttggctaaga aacaagaaga aaaaaagaaa caaataccgc aacagcggac aaaaaaagga
    20461 gtactagcgg gtatcttagg attggttacg aatctgatct tgtttgtctc taagctaatg
    20521 atcgggcttt tttcaggaag tgtctccatt atggctgatg cagtgaacag tctatcagat
    20581 actgcttcat ccatattgac tttgatcgga tttaaaattg ctgcaaagcc agcagatcag
    20641 gaacatcctt atggacacga acgcttcgaa tatatcagtg ggttatttgt ttcgatcatc
    20701 attacttatg taggatttca atttttagag tcttctgctg aaaaaatcat ccatccagaa
    20761 aatgtctctt taacacccat cgtctttttt gtcctaatca tttccatctt tttgaaattt
    20821 gcacaaggca aaatgtatca ggttattgcg caatccattg attctgaaac attgagagct
    20881 acagcaacag atagttataa tgatgtcttt actacactgg ctgtattgct ttcagcagca
    20941 atcgaatggg taactggatg gcgtattgat ggatatatag gttttgcttt agcattatat
    21001 atcctttata gtggattgac gatgcttcga aattttgtat acgaactatt aggaagtcgc
    21061 ccaacgccag cagagattga agaaatggaa gagcatttaa atgcttgcga tgatatactt
    21121 ggctatcatg atttattggt gcataactat ggacccaata aacgctttgc atcagtccac
    21181 atcgaagtag atgaatcaat gaatttgaat gtggctcata agattattga tctgatagaa
    21241 aaagacttta aaaagtcttt ggatgtggaa cttgtctgtc acttggatcc aatgcctgtt
    21301 gataacgaaa aatactataa tatcttgcgg cagttaaagc aaattcttct tgaaacaggt
    21361 acagggctta atattcatga ttttagagta attcatcaag gaaaaacgct gcaatttgat
    21421 gtagtggtcc ctgagaaatt ccagtttgtt gatacagaac tagaaagaat cttacgtagt
    21481 caaatagaag aaaaaatcgg agattaccaa ttggaaatta cgtttgatca caattatctt
    21541 ttatattaaa tgagaaacta taaaaagact ccttaaagtt tttaatttaa agagtctttt
    21601 tttcttttaa ttgtttatgg agagcctgac gtgcaagatt atctgctcct ttatttttgt
    21661 tttcgggcaa ccattgcagg atcaataaag aaaattcgga tagaagttct tggattcttt
    21721 gaaggtaggg aaggaagagc tcgttcttcg ttgcgttttt gtcgaccgta gaaactagtg
    21781 ttttgctgtc tgaaaaacag aaaatcgttt cattctgtaa gtcgtttttc tttagaaatt
    21841 ctaaagtttt taaaaagact tcgaactctg cttgatggtt tgtccctata gaaagcggga
    21901 tagatagctg ttcttgttta ccttgataga caatcaagat tccacctcca ctaggaccag
    21961 ggttgccttt tgtagaggca tcaatgtata cttttaacat aaaaagagat tctccttttt
    22021 gttcaaacta atgttatggt aagattatat cattactgaa tcggtgagag gttagagaat
    22081 gaaaaaaaat attcgatggc agcctgaact ggcacatacg atcatctatt ggtcattgac
    22141 cttgatcatt ctgtttttta gtctgattct ttctttagaa aacacaggtc cttattggaa
    22201 aagcaatttt gttttaggta tttttcttgt ttttctattt ctaggtttgc ggcgttcttt
    22261 tattttaaaa tcaaactctg tgattatacg ttatgctgct ttttggaaag accgagaaat
    22321 atctgtcgga cagattcgat ctttgacgcc aactaatact ggggtgcaaa tgatgttgca
    22381 agatgagaaa gccccatctg ttttcatgat gcgaaaaaaa gatcaagtag tcctttttga
    22441 tcattttgaa aaaaacggac ccaaatcaga atcaattcct tatccttata cgaaaaaaag
    22501 aaaaagctaa ttttaaaaat cagaacctaa aaaaacgaat ttttacagga aaagaaaaga
    22561 gtgagacgta cggttcatcc cgtttagcgt ctcactctat ttgtttatct attcatgcat
    22621 actagtatcg gtagtctgaa cgttgccttt cttgacatgg gcagcttgtg ttcctcgatt
    22681 cccttcgatg atctcaaatt cgaccacttg attttcttcc aaagttttga atccctcttc
    22741 ttcaatcgca gtaaaatgta caaaaatttc ttctgtgtca tcataactga taaagccgta
    22801 tccttttttg ttgtcaaacc attttacaat tcctgtggtc aaagcactca cgacctttct
    22861 aaagtctgat catagccttc attataaaga gtatttataa gtacgtcaaa taaattaaaa
    22921 acctgtaatt ttatgttttt tttctgaata aaaaaaggta taattggcat atcttaacaa
    22981 agaagatatt ttgttgaata aagttcgtga aaaggggtaa tcaaatgaag aatgatctgc
    23041 aaatttcaca agaaacagag ctagaaccga ttgttgaagt ggctagtaaa ttagatctta
    23101 aggaagacga tttggaactg tatgggaaat ataaggcgaa aatcaatttt tctggaatca
    23161 atcgatcaaa ggataacgct gaggggcatt tgatcttagt tacttccatc aatccaacac
    23221 cagctggcga aggaaagtca acgatcactg tagggttagg agacgcgctc aataaaatca
    23281 ataaaaaatc tgttattgca ttgcgagaac cctcacttgg tcctgtgatg ggaatcaaag
    23341 gtggtgctgc aggcggagga tatgcacagg tattgcctat ggaagatatc aacttgcatt
    23401 ttactggtga catgcatgct atcacaacag ctaataatgc attatctgct cttttagata
    23461 atcatattca tcaaggaaat gaattgaaca tcgatgcccg ccgagtgatt tggaaacggg
    23521 tagtcgatct caatgatcgt gaactgcgta aagtaattgt tggcttgggt gggccaatcc
    23581 aaggtgttcc aagagaagat gggtttgata tcacagttgc cagcgaaatc atggctatct
    23641 tgtgtcttgc ggcagacctt gaagatttaa aggctcgtct tgcaaggatc gttgtcggct
    23701 atacatttga ccgtcagcct gtgactgctg gagacttgaa agcagaaggc gctctggcat
    23761 tactgctgaa agatgcaatc aagccgaatc ttgtacaaac aatttacgga acaccagctt
    23821 ttgtccacgg gggaccgttt gccaatatcg ctcatggctg caatagtatt ttagctacca
    23881 agacagcatt gagattagca gattacgttg tcacagaggc aggatttggt gcagatcttg
    23941 gcggagagaa atttttagat atcaaagtac ctaacttgaa aaaagcgccg gatgctgtgg
    24001 tgattgtcgc cactgtccgt gcattgaaaa tgcatggcgg aatgaaaaaa gatgaactta
    24061 aaaatgagaa tcttgatgct ttgaaagtag gttttgcaaa tttaaagcgc catatacgta
    24121 atatggaaca atatcagcta cctgtgatcg tcgcaatcaa tgaattcgta acagatacag
    24181 acaatgaact cacactgttg gagcacttgt gtgaaaatca ggggattctt gccaagagag
    24241 cgagtgtatg ggcagatgga gcagaaggcg gcgttgattt agcaaaagct gtagtacgat
    24301 tgattgatag aaaagaagct aattacaagc cattataccg cctagaagaa acgatacaag
    24361 aaaagacaga aacaatcgtc aagaaaattt acggaggaaa tggtgttgtt ttcagcaaaa
    24421 aagcgcaaaa acaaatagaa gagtttacga agaatggatg ggacaaactg ccgatttgta
    24481 tggcaaaaac acagtattca ttttctgatg atccttcact gctaggtgca ccggaagatt
    24541 tcaccatcac gatccgcgaa atcatcccaa aattaggggc aggattttta gtagccttga
    24601 ctggagatgt tatgacgatg cctggtttgc ctaaaaaacc tgctgcattg aatatggacg
    24661 tgacaaatga cggagaagtg ttgggattgt tttaagcttg attataatct accatcgaag
    24721 gagctgtatt ttgactacag cttctttttt tagacgatag ggaattaatt ttgtttacac
    24781 taggatcatt ctttttcttt attgtatttt gagctatact aaagcataat aggagggtat
    24841 gatgaaaacg acatttataa gaaaaaccgg tttttacggt atgggtagct cgattcagct
    24901 aagaatcaac aatgaaaaac gcagtgttct cagtcatgaa caaaagataa cgcttgatct
    24961 ggaagtgccc tttacgatgc aggtcacctt ttattggcta aaaagccccg tttatacagt
    25021 gaaagaagca aagcctgctt atgtgatcac aatgaattac ttgattcttc aaatctatcc
    25081 gtttttattt ctagcaactg ggttgggggc cgttttccta caaaatttat ttttcagttt
    25141 ttttgcagtt attgtgttga ttgcgttctt tttctatata aaaaaccggg catatttgat
    25201 caaggaggaa aattatggca aactctgatc tttttttaat cagtttcaat cgaatcgaaa
    25261 aatggatgag agacgagatg ggaaatgcac gaaatatggg atttaccgaa cttgtcagac
    25321 gcctagctca aagaaaacaa ttaatgatca gaaaatacga agatgatctt ttacagcttg
    25381 ctcagttaag gaatgcgatc gtccatgatc ggatcgcagt cgattttgtc attgcagagc
    25441 caaatgattg gacagtcaag cgaatccagc tgatcgaaca agaactgatt cagcctgaaa
    25501 ccgtgctgcc tcgatttgca aaacgtgtaa caggttttga acaagatctt ccgttacttg
    25561 aactgttaaa gatagtagca gaaaaaaggt attcacaatt tccgttatac aataaaggaa
    25621 aatttgtagc attgattact ttacgtaata tcgggttttg gctagcaaaa gaaagccaaa
    25681 aaggtccggt tgatttaaca aataaacgag cgttggattt gatcatccaa aacggaaagt
    25741 acacaaatta tcattttgtt ccagctaaaa cacatattta cgaggttgaa gcgatgtttc
    25801 gtgagcaagg gactttggaa gccgtactta tcactaaaga tgggaatccc gatggtaatc
    25861 tgctcgggat cgtacgacct cgtgatattt ataaacaaat agaaaaggat tgattttttg
    25921 ttaattattt attggatatt cagcgctttg ctagtaggag tggatcagtt ggtcaaatgg
    25981 tggatcactg ataatctagc gcttggagag acgaaaaatc ttataccagg tattttgtca
    26041 ctgaatcata ttcgaaacac tggagccgca tggagtatgc tagaagggaa gatgtggttc
    26101 tttactatag tgacactcat tgcagtcgtt gtgattctca cattgatgat taaaaatcgt
    26161 gaaaaaggta atcgctggtt catgattgga ttgagtttga ttctagctgg tgcgattggc
    26221 aattttatcg accgtattcg tcttggctat gtagtagata tgttccagac agattttatg
    26281 agctttccga tttttaacgt agcagatgtg acacttgtat gtggagtgat ttgtatgtta
    26341 atttatatta ttttagatga aaaggatcaa agaaaaaaat gacagaactt aaaacgacga
    26401 ttcaccatga atctggtagg atcgataaag tattgactaa tttattcacc gattacagtc
    26461 gttcgcaaat tcagctgtgg ctgaaaaatg gagcggtttc tgtcaatgga cagccagtca
    26521 aagcaaatta caaagtaaaa caaggagacg agattactgt cttgatcccc gaaccagaaa
    26581 cattagatgt ggtcccagaa aatatcccgt tgacgatcgt ttacgaagat gaagatgtag
    26641 cggtgataaa caaacctcaa ggaatggtcg ttcatccttc agcaggtcat acgcatggca
    26701 caatggtcaa tgcgctgatg taccatatca aggatctttc tacgatcaac ggcgttattc
    26761 gtccaggtat tgttcaccga atcgacaaag atacttccgg actgctaatg gttgccaaaa
    26821 atgaccaggc acacgaatca ttagctcagc aattaaaaga taagacctct ttgcgcaaat
    26881 atgttgcttt agtccatgga gagattcctc acgaaaaagg aaccatcaat gcgccaatcg
    26941 gccgttcgaa ggaaaaccgg aaaatgcagg cgataagaga agatgggaag tcggctgtca
    27001 cacattttac tgtattggaa cgtttcgaag gatttactct ggttgaatta aaactagaaa
    27061 ccggaagaac acaccagatc cgtgtgcata tgaagtatat cggttatccg ttagctggag
    27121 atccagtata cggaccgaag aaaactttga aaggcaatgg tcagtttctc catgcgcaaa
    27181 tgttaggttt tacgcacccg actacaaacg aaaagatgat ctttgaagca cctcttccag
    27241 aagtttttga aaaaacttta gaaaaattaa gaaaaaacat tgcattttaa tttagataac
    27301 ggtatagtaa ttaacaacga aagcataaaa cttaatagaa ttcctttaag tttagtcccg
    27361 tgaggctaag aagggtggga gcaaggatac gtatggtgcg ccataagtag attgctcatt
    27421 gacatagtat agctatcagt gtatcagtat acgctcagtc taaacattcc tcctgtcctc
    27481 ttagggcagg agttttttta tgcaaaaaag tagggggaat gaaaatgcaa gcaaaagaag
    27541 tagtagatca ggtaaccatg aagcgagcat tgactcgaat tacttatgag attatcgaga
    27601 gaaaccatag tatccaagat atcgtattgg taggtatcaa aacacgagga atctacattg
    27661 catcaagaat tgccgaacgc ctgaagcagc tagaagatat cgatattcct gtaggagaac
    27721 tggatatcac tttataccga gatgacaaaa aagaaaatcc agaagaaccg gaacttcatt
    27781 cttctgacat cccagtttcc ttagaaggaa aagaagttat tttgattgat gatgttttgt
    27841 atacaggacg aacgattcga gcagcaatgg acgcagtaat ggattttggc agaccaagaa
    27901 aaatttcatt agcagtcctg gtcgatcgtg ggcatcgaga attgccaatc cgtgcagatt
    27961 atgttgggaa gaacattcca acatctcggg cggaagagat tttagtagag atgcaagagc
    28021 ttgatggaca agatcgcatc atgattttga aagaggaaga ttagaggaga gagaaacatg
    28081 tcggagaaaa aaggatttca taatgatgac gtggtgttag atatccacga tcgcccaacc
    28141 ccaggacatt gggtaggatt aagtttacaa cacttgttca cgatgttcgg agcaaccgtg
    28201 ttagtcccca tcttagtagg tatcgatcca ggtattgcgc tagtcagttc aggactaggt
    28261 acattggtgt acttatttac tactaaagga aagatacctg cgtacttagg aagcagcttc
    28321 gctttcattg cagcgatgca gcttttgatg aaaacagatg gttatccagc gattgcacaa
    28381 ggagcagtca caactggatt agtctatttg attgttgcgc tgatcatcaa aaagatcggt
    28441 tcagcatggc tggataaaat cttgcctcca atcgtcgtcg gtccagtcat catggttatc
    28501 gggttgggac ttgcggcaaa tgcagcaaca aatgctatgt ataatcaggg cacgtatgat
    28561 ttcaagtata ttgctgtagc gctgatcaca ttaggattaa caattttctt caacatgtgg
    28621 ctaagaggtt ttctcggatt gattccgatt ttactaggta ttattagcgg atacatcgta
    28681 gccttgctgt ttggaatcgt ggatactaag ccaatcgtag acgctgcctg gtttgctgtt
    28741 cctaactttg agattccctt tgtacaatat gaaccaaagc tatatcttgg agcaatcaca
    28801 acaatggcac ccatcgcttt tgtgacgatg acagagcata tcggccatct tatggtattg
    28861 aataaactaa ctaaacgtaa tttctttgaa gaaccgggtc ttcataagac gttgacaggt
    28921 gatggattag cacaaatcgt ggcagccttt gtcggaggtc ctcctgtaac aagttacggt
    28981 gaaaacattg gtgttctggc tatcacacgt gtccatagcg tgtttgtgat cggtggagct
    29041 gcagtatttg cagtcatttt aggattcgta ggaaaattaa gcgctttgat tttaagtatc
    29101 cctggaccag tcatctcagg aatcagtttt atcttatttg gagtaattgc cgcaagtgga
    29161 atgaagattt taatcgagaa caaaatcgat tttgataaaa agaaaaatct gttgattgct
    29221 tcagttatct tagtagtagg aattggcgga ctcgtctttg aagtaggcac atttacattg
    29281 tcggcaatgg cattagcaac tgtacttggc atcattttaa atcttatctt accagaaact
    29341 tcacgaagcg aagaaaacta aggaggaaac attatgatca tcaaatcaga acgtatcagc
    29401 ttaaagcatt tattgaccgt tgaggcactt actgatcaag aagttatggg cttgatccgc
    29461 agagggcagg aattcaagca aggtgctaat tggactccgc aaaagcgcca gtactttgct
    29521 acaaacctct ttttcgaaaa tagtactcgc acacataaaa gcttcgatgt agcggagaaa
    29581 aaactagggc tagaagtgat tgaatttgaa gcaagcacaa gctctgtcca aaaaggggaa
    29641 acactatacg atacagtact gacgatgtca gcattaggcg tagacgtagc ggttatccgt
    29701 catggtgatg agaactatta tgacgaactg atacaaagta aaacaatcca gtgttcgatt
    29761 atcaatggag gagacggaag cggacagcat ccaacccaat gcttgcttga tttgatgacc
    29821 atttatgaag aatttggtca ttttgaaggt ctgaaagttg ccatcgtagg tgatatcact
    29881 cactctcgcg tagctaaatc caatatgcag atgttaaaac gtctaggcgc acaagtattc
    29941 ttctcaggtc ctagagaatg gtacgacgat gaattcgaag tgtacggaca ttatatgcca
    30001 ttggatgaac tactagatca agttgatgtc atgatgatgc ttcgggtgca acacgaacgc
    30061 catgatggaa aagaaagttt ctcaaaagaa ggctaccatc aggaacatgg gttaacagtt
    30121 gagcgtgcga agaagttgca aaaacatgca atcattatgc acccagcacc agttaaccgt
    30181 gatgtagaat tagctgattc attagtggaa ggattacagt cgcggatcgt tcagcaaatg
    30241 agcaatggag tattcgtcag aatggcaatc ttagaagcag tgctaaatgg aaaagcgtaa
    30301 ttataggagg atgaaaaaat gaaaacactc attaaaaacg gtcagatcaa tacacgaaaa
    30361 aatatgacga caccagcaga aatttggata gaagatgggc gaatcaaagc aatcggtaca
    30421 ggattctcag aagccgaatt tgatgaagtg tttgatgcaa agggacaatt gatcacacct
    30481 ggtttagtgg acgtccatgt tcatttgaga gagccaggat ttacatacaa ggagacgatc
    30541 gaagctggga ctcgctcagc tgctcgtggc ggtttcacta ctgtatgcgc gatgccgaat
    30601 ttaaatcctg tacctgatac ggcagaaaag ctgagacaag tatatgacat cattagaaaa
    30661 gatgcagttg tgaaggtatt gcagtatgcg ccaatcacag aaaatctgcg aagtgagaaa
    30721 ttagttgatc aagaagcact gattgaagaa ggtgcttttg ctttcacaaa tgacggtgta
    30781 ggtgtccaaa cagctgggac gatgtatctg gctatgaaag aagctgcaaa aaacaataaa
    30841 gccctagtag cccatacaga agatgaatct ttattatttg gcggcgttat gcacgctggc
    30901 aagaaggcag aagaacttgg tcttcccggg atcttaagcg taaccgagtc ttctcaaatt
    30961 gctagagatc tgttgctagc tgaagcaact ggtgttcatt accatgtttg ccatgtttct
    31021 actaaagaaa gtgtccgtgt aatcagagac gcaaaaaaag caggaatcca cgtaacagca
    31081 gaagtttcac cccatcatct tattttgatc gacgaagata ttcccgaaga tttcggcttt
    31141 tggaaaatga acccgccgct aagaggacgg gaggacagag aagctttgat cgaaggcttg
    31201 cttgacggta cgatcgattg catcgctaca gaccatgccc cccatggatt agaagaaaaa
    31261 agccaaagtt tcatgaaatc accatttggt atcgtaggaa gcgaaacagc gtttcaactg
    31321 atctatactc attttgtaga aactggtcga ttcacgttgg aacaagtgat caattggttg
    31381 gcagttaaac cggctgagat ttttggtttg aatgcaggaa cgctaacggt tggtgccccc
    31441 gctgatgtag cagtttttga tattacacaa acttgcacga tcgataaaga agacttttta
    31501 tcaaaagggg agaatacacc ttttatcggc tggaaagtaa aaggcgaaac acaaatgact
    31561 tttgtcaacg gaaaacttgt gtggcagaag ggagaataaa agtatggaac gtttgcttat
    31621 attagaagat ggaacagttt tcgaaggaaa ggcttttggt gcagatgcta gtgtcttcgg
    31681 agaaatcgtt tttacaacaa gtatgactgg ttatcaagaa actatcactg atcaaagttt
    31741 taacggtcaa atcattacat ttacctatcc tatggtgggt aattatgggg taaatagaga
    31801 tgattatgaa tcgatttctc caacatgcaa aggagtagta gtaaaagaac atgcacgtgt
    31861 agcttcaaac tggcgaaacc aaatgacgct agattcattt ttgaaacaaa aaggaattcc
    31921 tggaatatca ggtatcgata cgcgcgccct tactcgcaaa atccgtgaag tcggaacgat
    31981 gaaagctagt atcgttgatg ctggagattc cttcgaacat gcatttgatc agctgaaagc
    32041 aaccgttcta gcaaccaatc aagtgcagca agtctcaact aacaaacctt atccaagtcc
    32101 aggaacaggt cggaaagtca ttgtcattga cttcggcttg aaacatagta ttttacgaga
    32161 attgagtaaa agaaactgta acctcacagt acttccctat aacacagatg ccgagacgat
    32221 tttagcgcta tcaccagacg gagtcatgct gacgaatgga cctggtgatc caaaagatgt
    32281 gccagaagca atcgaaatga tcaaaaaaat ccaaggaaaa gtaccgattt ttggtatttg
    32341 tctcggtcac cagttatttg ctttagccaa cggtgcagat acctataaaa tgaaattcgg
    32401 acatcgagga ttaaatcacc cggtacgaga agtagcgacc ggaagaattg attttacttc
    32461 tcaaaatcat ggctatgcag tcgatgaagc cacgattgat ccagaaaaac tcatcgtaac
    32521 tcatgtagaa gtaaatgacg gaacagtaga aggagtcaaa cacagagatt atcccgcttt
    32581 cagtgtgcag tttcatccag atgctgcccc tggtccgcat gatgcactac atttatttga
    32641 tgaatttatg gaaatgatgg acgcaggaaa ggagcagtaa acagatgccg aaacgtacag
    32701 atatcaagaa aattatggtg attggatctg gtccgatcat cattggtcaa gccgcagaat
    32761 ttgactacgc aggaacacaa gcctgccttg ccttaaaaga agaaggatat gaagtggttt
    32821 tagtaaattc taatcctgcg acgatcatga cggataaaga agtggcagat caagtatata
    32881 tcgaaccgat cacacttgaa tttgtatcaa ggattcttcg aaaagagcgt ccagatgctc
    32941 tccttccaac tttaggcgga cagacaggcc tcaatatggc aatggagctg gcagcatcag
    33001 gtattttaga agaattgaac atagaacttc taggaacaaa gctttctgcg atcgatcaag
    33061 cagaagatcg tgatctattc aaacagttga tgaaagaatt gaatcagcca atccccgagt
    33121 ctgaaatcgt caatacagta gaagaagctg tagattttgc aaataaaatc ggctatcctg
    33181 tcatcgttcg tccagccttt actttaggcg gaactggtgg aggtatgtgt gaaaatgaag
    33241 aagaattacg tatcgtatca gaaaatggat taagtctttc ccccgctacc caatgtttga
    33301 ttgaacgaag tattgcaggt tttaaagaaa ttgaatatga agtgatgaga gattcggctg
    33361 ataatgcaat cgttgtctgc aatatggaaa attttgaccc ggtgggtatc catacaggtg
    33421 attctatcgt atttgcccct agtcaaacat tgtctgacca tgaatatcaa atgcttcgtg
    33481 atgcttctct ttcgattatt cgagcactta agatcgaggg aggctgtaat gtccaactcg
    33541 ctcttgatcc acatagtttt aattattatg tgattgaagt caatccgcgt gtttctcgtt
    33601 catctgcgtt agcaagtaag gcgactggct atccgatcgc taagctagct gctaaaatag
    33661 cgcttggatt aacattagac gaaatgaaaa atcctgttac aggaacgact tttgcagaat
    33721 ttgaacctgc attagattat gttgtagcta aaattcctcg ctggccgttt gataagtttg
    33781 aaaacggaga acggctttta ggcacgcaga tgaaagcaac tggtgaagtg atggctatcg
    33841 gtcggaatat cgaagagtct ttattgaaag ctgtgcgttc tttagaaatc ggtacacacc
    33901 atatcgaact tcctgagttg gcagacatca cggacgatga cctaatccaa aaagtggttc
    33961 gagcacaaga tgatcgcctg ttttatgtag ctgaagctat tagaagaggg tacaccatag
    34021 aagaattagc ggatttgaca aaaatcgatt tattcttttt agataaactg ttgcatatcg
    34081 tagaaatcga acagcagttg aaacagcagc cgttggatga caagatattg aaaacagcga
    34141 aacaaaacgg ttttacagac aaaaaagtgg cggagctgtg ggaaataaac gaagaagccg
    34201 ttcgccagat gagaaaagaa aatgatttga ttcctgtata taagatggtt gacacttgtg
    34261 cagcggaatt cgaatcaaaa acaccttatt tttatagttc ctatgaatat gaaaatgaaa
    34321 gttcacgatc agaaaagccc tcaattctag tactgggatc tggtcctatc cgtatcggac
    34381 aaggggttga atttgactat gcgactgtgc attctgtcaa agcaatccag caggcaggat
    34441 acgaagcaat catcatgaac agcaatcctg aaacagtttc gactgacttt tctatttcgg
    34501 ataaattata ttttgagcca ttgaccttcg aagatgtgat gaatgtgatc gagctagaac
    34561 agccgatagg agtaatcgtt caatttggtg gtcagacagc aatcaactta gccgaacctt
    34621 tgactgctgc tggtgtaaaa atcttaggga caacgatcga agacttggat cgtgcggaaa
    34681 atcgtgattt attcgagcaa gcgctgaaag agttggatat tcctcaacct ttaggggata
    34741 ctgctacaag taaagaagaa gccgtggtta tcgcttcgag aattggttat ccagttcttg
    34801 ttcgtccaag ctatgtatta ggcggacgtg cgatggaaat cgttgaaaac cagcgagatt
    34861 tagaagatta catggaacat gctgtgaaag catctccaga acatccagta ctagtggacc
    34921 gttacttgct tggtggtgaa tgcgaagtgg atgcgatttg tgacggtgaa acagtactca
    34981 tcccagggat catggagcat attgagcgtg ctggtgtcca ttcaggggat tcaatggctg
    35041 tgtatccacc tcaaacatta tctgaatcga tcaagaaaac gatcgaagat tacacgatcc
    35101 gcttagcatt aggtttgaat tgtgtcggga tgatgaatat ccagtttgtc atccatgaaa
    35161 atgaggttta tgtgatcgaa gtcaatcctc gagcaagtcg gactgttcca ttcttaagta
    35221 aggtgacagg aattccaatg gcacaaattg cgacgaaagc tattttggga gagaaactaa
    35281 agacacttgg ttatgaaaac ggactttatc cagaaacaaa agaagttcat gttaaagcac
    35341 ctgtcttttc ttttacgaaa ttgcagaaag ttgatactta cttagggcct gaaatgaaat
    35401 cgactggaga agtaatggga tctgacagac aattagacaa agctttgtac aaggcattcg
    35461 aagcttctgg attacacttg cctgaatatg gagccgtatt atttacgatt gcggacgaaa
    35521 caaaagaaga agcactagaa ttagctaaac gttttacaga aattggctat agtctgatag
    35581 gaacgaaaca tacagctgct tatttcgaaa aaaatggtct ggtcgttgaa acagtagcga
    35641 aaatttctga ggaagcagca gaaaaaaatg tagtcgactt aataagagat ggtaaaacac
    35701 aagtggttgt caatacgatt gataaagatc ggggaaatgc ttctaaagac ggtttcatca
    35761 tccgaagaga agcagttgaa catggtattc cattgtttac ttctttagac acagcagatg
    35821 cgatcgttcg agtgatggaa tcccgtgctt tttcaactaa ccctatttaa cggcgagcac
    35881 atgttaagac aaaaagacga aaaaccgttg caggagggga cagatgaaac aagaaatgat
    35941 ggaaatcgtt cagcaaaaac agctggcacc aaagattttt gaaatgacat tgaaaggtga
    36001 tctagtaaaa gaaatgcagc aaccaggaca gttcttgcat attctcgtgc cgcgtgcaga
    36061 cttgctatta cgccgaccga ttagcttgaa tcatatcgac cacgaaacaa atacttgccg
    36121 tatcatttat cgggtggaag gtgaggggac tagagtcttc tctactcttt ctgccggaga
    36181 ccatttagat gtcatgggtc ctttaggcaa tggttttgat ttgtccaatc ttcatacagg
    36241 agacgaagta tttataatcg gtggaggaat cggtgtccct cctctatatg agttatcaaa
    36301 acagttagtc gaaaaaggaa ttaagccaac gcatttttta ggttttgcca cacatgaagt
    36361 cgtttattat gaaaaagaat ttaatcgtct aggagaaaca agaatcgcta ctgacgatgg
    36421 aacatacggt atgcacggaa atgtcggtaa tttattgtta ggaacgaaga cggaacctac
    36481 agctgttttt gcatgtggtt caaacgggtt attgaagaca gtggaacagc tgttttcttc
    36541 gcatcaaaat gtccagttat cattagaatc ccgcatggca tgtgggatag gggcttgcta
    36601 tgcttgcgtt tgtcatacgc cggaagatga aacaggaaca aaaagtgtga aagtttgtga
    36661 cgaaggaccg atttttaaaa taggagaggt aattatatga atgagaatcc tttagcggtc
    36721 aaacttcctg gcctagaatt aaaaaatcct atcatgccgg caagcggctg tttcggtttt
    36781 ggcaaagaat acggaaaata ctatgatttg aatcagcttg gatcgatcat ggtcaaggca
    36841 acgactccaa atgcccgctt tggcaatccc acgccaagag tggcggaaac accatctggc
    36901 atgttgaatg caattggttt gcaaaatcct ggtctagacg ttgttatgca taaacttctt
    36961 ccagaattag aggagtacaa tgagttaccc atcatcgcaa atgttgctgg tgcttgtgaa
    37021 gaagactatg tagaagtttg tagcaaaatt ggcgaagcac caaatgtgaa agcaatcgag
    37081 ctgaacattt cttgcccaaa tgtcaaacat ggcggaattg cttttggaac agattctgat
    37141 gttgcttttc aattaacaca agcagtaaag aaagtttctt ctgttcctgt ctatgtcaaa
    37201 ttgtccccaa atgtaacaga catcgttcca atcgctcagg caatcgaagc tggaggggca
    37261 gatgggttta cgatgatcaa tactcttctt ggtatgcgga ttgaccttaa gacaagaaaa
    37321 ccgattttag ccaatcaaac aggtggactc tccggaccag caatcaagcc tgtcgctatt
    37381 cgtttgattc accaagtagc aagtatatcc agccttccaa tcattgggat gggcggcgtg
    37441 cagacagtgg atgatgttct tgagatgttt atggctggag caagtgctgt agctataggg
    37501 accgcgaatt tcaccgatcc gtatatctgt cctaaactaa ttaaagagct ccctgtgcga
    37561 atgagcgaac taggcatcga atcgctggaa agattaatca aagaagtgag agaggaacgt
    37621 gaacgatgaa tcaactagac caacgaccaa ttattgcatt agatttttca actaggcaag
    37681 aggtagaaga ctttcttcga tttttcccga aagaagaaaa attgtttgtg aaaatcggaa
    37741 tggaattgtt ctaccaagaa ggacctgaaa tcgtacgtta tctaaaagag gctggtcata
    37801 atgtattttt agatctgaaa cttcacgata ttccgaatac ggtcgaaaaa gcaatgcggg
    37861 gattggcaaa gcttggtgtt gatctcacct gtgttcatgc cgctggcggt attcgtatga
    37921 tggaggcggc aatgcgagga ttagaagagg ggactccaga aggtggaaaa cgtccgctac
    37981 ttctagctat cactcagctt acttctacaa gtgaagaaga aatgcatgcc gatcaactaa
    38041 tcgaagtttc tttagaaaaa agtgtgatcc attatgctag ctgtgccaaa aaagcaggat
    38101 tagatggtgt ggtttcttct gcttgggaag tagaagcgat caaagaaatg gcaggagacg
    38161 aatttgtttg cttaactccg ggtataagac cagaaggaac agtcgcagga gatcagacaa
    38221 gagtagtcac accttctcag gcaaaaaaga tcggctcaac ttttatcgtt ataggtcgac
    38281 caatcactca gtcaactaat ccttataaag catatcaaac gattcaaaca gaatggagct
    38341 aacctaaaat gaatgttgaa caaagtatcg caaaagactt attagaaatt gaagcagtat
    38401 ttttaaatcc aagtaatcct tttacatggg caagtggaat caaaagccca atttactgtg
    38461 ataaccgaat cacgatgagc tatccaaaag tccgaaaaga gatcgcaaaa ggattagcaa
    38521 gcaaaatcaa agaagcttac cctgaagtac aagtgattgc tggaactgca acagcaggaa
    38581 tccctcatgc cgcatgggta gcggaaatac tggatttgcc aatggtctat attcgaagta
    38641 aggcgaaaga tcatgggaaa ggtaaccaaa tcgaaggacg tatatttgaa ggtcagaaga
    38701 tggttgtgat cgaggatttg atttctacag gcggaagtgt cttggaagca gctgaagcag
    38761 caaaaagaga aggtgcggat atccttggtg ttgcggctat ctttacgtat gagttgccaa
    38821 aaggaaaagc aaatttcgaa aaagccgaaa tccctcttat gacgttgacc aattattctg
    38881 tattgattga agcagcttta gaagatcgtt atatcgatga gcaagaatta actctgctaa
    38941 aagaatggaa aaaagatcct gaaaattggc agatttaagc gcgcttgatg aagatagaga
    39001 aatccgattt gtgtctccag aatcatcaat tggaatagca aaaatccaaa aactgattgc
    39061 gagaagcatc ttttcatcag gtatgaaaag aaacttctat cgtgatcagt ttttgatttt
    39121 tttaaaattc atttttctaa gactggttag cagaaagaag agaataatct cttctctttt
    39181 ttctattata tatagatttt ctctattttt tggtaaggga gctgtttcaa aaatggtaaa
    39241 gagcggaaat gttgcgaata tcagttattt aatgatatga tattcggaaa aagaaatctc
    39301 aaagggaggt aacttttatg gggttgaatg aatttatttt aggattgaac aatttagaga
    39361 cgatcacaag ggcacctgga ttttttaaat atacagagca tacagttgca gcacattcgt
    39421 atcgtgtagc atctattgcg caagttctag gagacattga agaagcgaat ggacaaccga
    39481 tcgattggaa actgctctat gaaaaatcat tgaatcatga ctacacagag cgttttattg
    39541 gtgatatcaa aacgccggtg aaatacgcaa gcaaagagtt aagagggatg ctgcaaacag
    39601 ttgaagaaaa gatgacggac gaattcatca accaagagat tccagctgag ttccaagaag
    39661 tttatcgccg tcggttgttc gaaggaaaag atgaaaccgt agaaggagaa ctattagcca
    39721 tttcagataa ggttgacctt ctttatgaat cttttgatga aatcatcaaa aataacccgg
    39781 aaaaagttta taaggagatg ttcattgagg cagtgactac gattaaaagc tttgaacatc
    39841 ggccttctgt tcagtatttc tttaaagaga tatttccgga actattgaac caggaatttt
    39901 atggaaaaga ggcgttttta gaaaccgtct caacgattgt aaagaaagaa gaaaaataaa
    39961 aatgatatga gatgcagggg gaagtatagt tgaaaaagaa tatggatgtc gaatggggct
    40021 attctggaga caaaggtccg gaaaattggg cgagtttatg tgattggttt gcgagaggtg
    40081 cggaatttcc attgcagtcg cctattcatt tgaccaaggg ggaagaaacc aaaatagaag
    40141 gagacacgct ttcctttcat tatcaaaagc aacggtttac tgacaaggaa ttcaaaaaca
    40201 cgattcattt agtacctttt gaccaactca gttatgtaga gttcaagaaa gaacgctatt
    40261 atttgacaga tattcatttc cacttgcctt ctgaacatat catcaacgga aaacaggaag
    40321 atattgaatt tcattttgtt catatgaaca gcaaaaaaga gaatctagtt gtgggtgtca
    40381 tgtaccagct aatggtacag gaaggttggc tgtataatca agaaaatggg gaaagttgga
    40441 acttagaaaa acatgagcat tgggtaaatc cggatgtgtt ctttccggag caaaaaagcc
    40501 atttccatta tattggctca ttgacaactc cgcctaccag cgggccaatc caatggtttg
    40561 taatggatca tgtaggaaaa ctgaaccaag agtttcttct ccccttcgat ggtcagtatg
    40621 ctcgaccgaa caaccgacca atccagccct tgaatggaag agaaatttat tatttcgagg
    40681 agtttgagca atgaatattc agcagatgaa atacgtggtc gctgtagcaa acaatggtag
    40741 tttcagagaa gcggcaaaga aaatgtttgt ctcccagcca agtctttcag ctggtatcaa
    40801 agaattagaa accgagttag ggatcacgct gtttactcga acgaatcgag gggcatattt
    40861 aacagaagaa gggcaagact tcttaaatcg tgccgagaaa atattggtcc agcttgatac
    40921 attagaaact cactatctaa cgagtgaaaa gagagacaat ttttcgatcg ctgcccagca
    40981 ttatgatttc ttaggtcctt tgaccgcaca gctactcgag gaattcaaag acgagatcaa
    41041 acaatttcgg atcatggaga cgacaacagc gagagtgatc gaagaagtca aagagcagca
    41101 cagtgagatc ggcattattt atatgaatga gcataatcaa gctggtatca gaagatattt
    41161 acaacaagga gagctaagtg gccgcagcat tggaaaattc aaaacgcata tttttttaag
    41221 gcaaggacat ccattagctg aacacgcaga gatcaccaaa gaagaattgt tatcttatcc
    41281 tcaagttcgt tttacacagg atggaaataa tttcccgtat ttttatgaag accttatcga
    41341 gatccctgat caagaaacag tgatttatac gagtgatcga ggtactttga tgaatatcgt
    41401 tcttgaaact gatgcttacg cttcaggatc tggtattgtg attggaggga ttcgcgagca
    41461 cttgcggtta atccctttgg cagaaggcga actaaatgaa ttttatatca tttattcggc
    41521 gaaacgtccg ctcagtgaaa tcgctcaaag atttatcaaa gagcttactc ggttactgga
    41581 ccagcagagc taagaaaagc agatgagaac gctgtttatt gaacaccgtt gatcaaaaaa
    41641 gaggggttgt gacagaattt tgtcacaact cctctttttg tagcaaacaa tttataacta
    41701 attgctgttg tgggaaagta cgcaaatatg attctagaaa cactcattga ttacctgttt
    41761 tgtttcatag ctgtaatttt ttcttcttct ggtgtcacga taatcgtctt ttggttttca
    41821 taaataacat aaccaggctt ggcgccattt ggttttcgga tatgctttac ctgtacaaga
    41881 tcaaccggca cttgggcaga gtatcggtat tttgaaaaat aggcagctaa ctcggcagct
    41941 tctgtaattg tttcatctga gggcttgtca cttttaatga tgacgtgcga accgggtata
    42001 ttttttgcat gaagccaata gtcggttttt ttagctgtct tcattgtcag ctggtcattt
    42061 tgaagattgt ttttaccaac aagaatgagc gttccgtcag aagaaaagta ttgatcaggc
    42121 tgagatggtt tcttacgttt ttgttttttt aagttcttct ttttcaggta tccttctgaa
    42181 gtcaattctt ctttaattgc ttcgatatcc attgggccgg caatttccag ttgagataag
    42241 acagattcaa gatattggat ttcatctttg gcttcttgga tttgttcgcc aatcagctta
    42301 acagcgttct tcagcttttg gtatcgatgg aaatatttct gtgcattttg atttggtgtc
    42361 aatgccggat ctagtgctat tttgattggc cgatcttctt cataataatt aggtaaaaca
    42421 acttcttttg cgccgcgtgg tacttgtgtc agaaaagtag taagtagttc gccatttcta
    42481 cggtaattct cagcgttttc ggattctttg agtgtttgtt cacgtttttt caatttattt
    42541 ttatttcgtt ttagttcatt ttcgattttt cgaatcagtt ctccgccttg ttgttttgcc
    42601 cgatctttct ctgctttttc gtgataatag ctatctaata attgactgag tgttggataa
    42661 gctgttacag cagatgcctg ttcggacaat acctgataag caatcggtgt gaaaaaatct
    42721 ttattttccg tttcgtaaaa agtaggaata ggctgatggc tgatggctga gaaaaattcg
    42781 tcccatacaa ccatcttttc attaggacgt tccagtaatc ttttggtcag ctcttgagcg
    42841 atatcgaaac cgattccctg aaactgacgt tggattgctt ttggatctag ttcagtttgg
    42901 gaaagccgct gaaagatttt ttctttctcg gagctaaatg ggttcaagac ctcttgtttt
    42961 ggcggagcaa catattctac accaggaagc aaagaacgat aagtgttttg agagcttcca
    43021 atgtgtttga tcgcatccag tatttttcca gtttctcgat taactaagac gatcgtacta
    43081 tgacgtccca ttaattcaac gatcaatatg atattttgta gatcacccaa ctcgtttcgt
    43141 ttagcaaagt ggaaatggat cactcgatca ttttcgattt gttcgatgtc ttctaaaata
    43201 gcgccatcca aatattttcg aagcatcatc acgaagttag gcggattatc aggattctga
    43261 taatcgattt gggtaatctg tacacgagca taacttgggt gcgcagataa taataatcga
    43321 tggtttttgc cgcgtgaacg aatcaccagc acgatttcat tttcataagg ctgatggatt
    43381 ttcgagatcc ggccagatag taacgtttca cgcaattcgt tgaccatcgc gtgggtaaat
    43441 acaccatcaa aggacatggt tcattcctca tttcttttca taagtatatt cattataacg
    43501 aaaaaaagaa gaaaaaccaa tttggccgaa tagtaaagca aaagtctaga aaaatcttgg
    43561 actaattaca taaaaaacag tgtttctatc gttgaaatcc aaatttttag gt
//
LOCUS       DAZKCZ010000010        39597 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000010 DAZKCZ010000000
VERSION     DAZKCZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.010"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..607
                     /locus_tag="ACG8KN_000483"
     CDS             <1..607
                     /locus_tag="ACG8KN_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187566.1"
                     /translation="FLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLLDHSDITE
                     AEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFYSFN
                     HNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLALM
                     PLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(741..866)
                     /locus_tag="ACG8KN_000484"
     CDS             complement(741..866)
                     /locus_tag="ACG8KN_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFP9187567.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYSILWEELAA"
     gene            complement(1000..1896)
                     /locus_tag="ACG8KN_000485"
     CDS             complement(1000..1896)
                     /locus_tag="ACG8KN_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFP9187568.1"
                     /translation="MNYYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKK
                     EFSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLS
                     VDEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRATAGNEKYRFITLSSIEHF
                     PLTLANYFFVQKNQLPVTKAFKELAELIGDVNEAYFFDQIEVIVDRIRKNKYKNRRPS
                     IFHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEETAFLKRQIFSELS
                     KKFIPHAFSYIKEYRTVLVSKT"
     gene            complement(2078..3004)
                     /locus_tag="ACG8KN_000486"
     CDS             complement(2078..3004)
                     /locus_tag="ACG8KN_000486"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFP9187569.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3089..3850)
                     /gene="pflA"
                     /locus_tag="ACG8KN_000487"
     CDS             complement(3089..3850)
                     /gene="pflA"
                     /locus_tag="ACG8KN_000487"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFP9187570.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGNKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4094..6325)
                     /gene="pflB"
                     /locus_tag="ACG8KN_000488"
     CDS             complement(4094..6325)
                     /gene="pflB"
                     /locus_tag="ACG8KN_000488"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9187571.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6602..9052)
                     /gene="parC"
                     /locus_tag="ACG8KN_000489"
     CDS             complement(6602..9052)
                     /gene="parC"
                     /locus_tag="ACG8KN_000489"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9187572.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     VTIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9064..11109)
                     /gene="parE"
                     /locus_tag="ACG8KN_000490"
     CDS             complement(9064..11109)
                     /gene="parE"
                     /locus_tag="ACG8KN_000490"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9187573.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLTVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEVPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11275..11709)
                     /locus_tag="ACG8KN_000491"
     CDS             complement(11275..11709)
                     /locus_tag="ACG8KN_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9187574.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEVADDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11836..12474
                     /gene="plsY"
                     /locus_tag="ACG8KN_000492"
     CDS             11836..12474
                     /gene="plsY"
                     /locus_tag="ACG8KN_000492"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9187575.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12606..13481)
                     /locus_tag="ACG8KN_000493"
     CDS             complement(12606..13481)
                     /locus_tag="ACG8KN_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9187576.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNREIFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADAVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13563..14354)
                     /gene="codY"
                     /locus_tag="ACG8KN_000494"
     CDS             complement(13563..14354)
                     /gene="codY"
                     /locus_tag="ACG8KN_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFP9187577.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14372..15772)
                     /gene="hslU"
                     /locus_tag="ACG8KN_000495"
     CDS             complement(14372..15772)
                     /gene="hslU"
                     /locus_tag="ACG8KN_000495"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFP9187578.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALIKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15786..16334)
                     /gene="hslV"
                     /locus_tag="ACG8KN_000496"
     CDS             complement(15786..16334)
                     /gene="hslV"
                     /locus_tag="ACG8KN_000496"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFP9187579.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16371..17276)
                     /gene="xerC"
                     /locus_tag="ACG8KN_000497"
     CDS             complement(16371..17276)
                     /gene="xerC"
                     /locus_tag="ACG8KN_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9187580.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17474..18850)
                     /gene="trmFO"
                     /locus_tag="ACG8KN_000498"
     CDS             complement(17474..18850)
                     /gene="trmFO"
                     /locus_tag="ACG8KN_000498"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFP9187581.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLDSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18819..20897)
                     /gene="topA"
                     /locus_tag="ACG8KN_000499"
     CDS             complement(18819..20897)
                     /gene="topA"
                     /locus_tag="ACG8KN_000499"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9187582.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDIVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21019..21876)
                     /gene="dprA"
                     /locus_tag="ACG8KN_000500"
     CDS             complement(21019..21876)
                     /gene="dprA"
                     /locus_tag="ACG8KN_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP9187583.1"
                     /translation="MYQIEENLLKLAYAKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITTHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVVEAGYVIVSGCAKGIDTYGQQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHRFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21932..22699)
                     /locus_tag="ACG8KN_000501"
     CDS             complement(21932..22699)
                     /locus_tag="ACG8KN_000501"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9187584.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22692..23552)
                     /gene="ylqF"
                     /locus_tag="ACG8KN_000502"
     CDS             complement(22692..23552)
                     /gene="ylqF"
                     /locus_tag="ACG8KN_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFP9187585.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23813..24211
                     /locus_tag="ACG8KN_000503"
     CDS             23813..24211
                     /locus_tag="ACG8KN_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9187586.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWVMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24448..24990
                     /locus_tag="ACG8KN_000504"
     CDS             24448..24990
                     /locus_tag="ACG8KN_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9187587.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRVIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25073..25624)
                     /gene="lepB"
                     /locus_tag="ACG8KN_000505"
     CDS             complement(25073..25624)
                     /gene="lepB"
                     /locus_tag="ACG8KN_000505"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9187588.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25776..26003)
                     /locus_tag="ACG8KN_000506"
     CDS             complement(25776..26003)
                     /locus_tag="ACG8KN_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFP9187589.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26000..26518)
                     /gene="msrA"
                     /locus_tag="ACG8KN_000507"
     CDS             complement(26000..26518)
                     /gene="msrA"
                     /locus_tag="ACG8KN_000507"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9187590.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26538..27251)
                     /locus_tag="ACG8KN_000508"
     CDS             complement(26538..27251)
                     /locus_tag="ACG8KN_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFP9187591.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27254..28150)
                     /locus_tag="ACG8KN_000509"
     CDS             complement(27254..28150)
                     /locus_tag="ACG8KN_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9187592.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28308..29150)
                     /locus_tag="ACG8KN_000510"
     CDS             complement(28308..29150)
                     /locus_tag="ACG8KN_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9187593.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEEILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29282..29935
                     /locus_tag="ACG8KN_000511"
     CDS             29282..29935
                     /locus_tag="ACG8KN_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9187594.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(29999..30526)
                     /locus_tag="ACG8KN_000512"
     CDS             complement(29999..30526)
                     /locus_tag="ACG8KN_000512"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9187595.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEIDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30546..31493)
                     /locus_tag="ACG8KN_000513"
     CDS             complement(30546..31493)
                     /locus_tag="ACG8KN_000513"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9187596.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKEVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31505..33388)
                     /locus_tag="ACG8KN_000514"
     CDS             complement(31505..33388)
                     /locus_tag="ACG8KN_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9187597.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSGDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33556..35628)
                     /locus_tag="ACG8KN_000515"
     CDS             complement(33556..35628)
                     /locus_tag="ACG8KN_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9187598.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRHADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            complement(35641..35916)
                     /locus_tag="ACG8KN_000516"
     CDS             complement(35641..35916)
                     /locus_tag="ACG8KN_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187599.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36278..36733)
                     /locus_tag="ACG8KN_000517"
     CDS             complement(36278..36733)
                     /locus_tag="ACG8KN_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187600.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36887..38095)
                     /locus_tag="ACG8KN_000518"
     CDS             complement(36887..38095)
                     /locus_tag="ACG8KN_000518"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFP9187601.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPSLKEEGEALLRFAELPSVPLKEESQVWTLLISQLGLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38207..38539)
                     /locus_tag="ACG8KN_000519"
     CDS             complement(38207..38539)
                     /locus_tag="ACG8KN_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9187602.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38687..39562
                     /locus_tag="ACG8KN_000520"
     CDS             38687..39562
                     /locus_tag="ACG8KN_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9187603.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
BASE COUNT        10616 a         8321 c         6812 g        13848 t
ORIGIN      
        1 ttttttacat ttaggctctt tttttgcaac tttgacgatc ttattttttc tatgttattc
       61 ataccttaag acctcacatg cgccttctag tgcattaggc tggttgcttt tggatcattc
      121 tgatatcaca gaagccgaag aaaatccgtt ttttttgatt ttgtcttctc tttgcggctt
      181 actgttttct gtttatttcg gtgcctattt ttttcatcga catcctggta cgttaaacga
      241 agcttcaagc gtattgattg catttaaaac gttttctttt ttagcaattc ttttttattc
      301 gtttaatcac aatacgcttt tcttctttat catgaatggt ttagtcgtga ttttatccat
      361 ttttacttat tttatttttt cactcatttt tagccaattg aaatgtccac tcttctttcg
      421 attagttcct gtcacctgtt ttatttacag cttgttttcc ttttttgtcc ttgctttaat
      481 gccactgacc aacccttcga cgatccaagt cgcaggaaat ctgttggata tgctcttcat
      541 cgtcagtcta ggtgtattta tgtggcaaag aaaaaacaaa caaaataaaa agcattttga
      601 agcctgacta ttacgacttc ataaatgctt ttttagtttt aaacacttca atctaaaaga
      661 ataacccaag attggataaa ttattaatga ctcgccattc gtttcttagt gattctggat
      721 catcaaatgg acttcctaat ctaagcagct agttcttccc ataaaatgct atacttgact
      781 gaatcataat attgattatt taaaaatcta acttttctta tcactacttc ctttgtcatt
      841 ccacatttct caccattttt ttgcattcct ttgtttccag accatgtagt gaatccaata
      901 ggtggtagat aggcaaaata ctcaaataat tcttttaacc ataaaggaag agaaaggctc
      961 ctcctgcaag agtcagtata atttttgctt actgctctct taggttttac tcactaaaac
     1021 tgttctatat tctttgatat aagaaaatgc atgcggtata aatttcttgc tcagctcaga
     1081 aaagatttgt cgtttaagaa aggccgtttc ctccattgtc aatgaaggat ccaatgttgg
     1141 taattcaatc tcaaaattgt ttttttcgac aataggtaat tctaatgtga atgattcttc
     1201 ttcctttata gtattcgtca caagtagaga ctgatggaaa atactaggcc ggcgattttt
     1261 atatttattc ttacgtatcc tgtcaacgat aacttctatt tgatcaaaaa agtacgcttc
     1321 atttacgtct ccaataagtt cggctaattc tttgaatgcc ttcgttacag gaagctgatt
     1381 cttctgtaca aaaaagtaat tagccaaagt aagcgggaag tgttctatag aggaaagtgt
     1441 gataaaacgg tacttctcat ttcctgcagt tgccctcagc aattctgtct ctcggctgtt
     1501 tcttactcca taaaaatacg cttctttccg ttcatcttcg aaacaatacg cccaaataac
     1561 ttctcccatc gctaattttt gttcatcaac agagagtctc ttcaattgtt cagctatggt
     1621 ttcagcagcc gaagtggctt gttgcaagta atctttagga tcaaatattg gaaagttcaa
     1681 atgatatctt cgttcttccc tcttgattaa tcctgcatcg atcaatagat caagaaaatg
     1741 ggaaaattta ttttgcgaaa attctttttt caattctctt agaatcgttt cttgttctcg
     1801 atgttcatat agataatcgg tcaatgaaga aaaaagcgga tcatcggtca gtttttgata
     1861 aaagttttcc ttattttttg aataataata gttcatatat gttctctttt ctatccgttt
     1921 tattttactt aaaaggcttt tacgtaaaaa ggaggaagaa caagagtcat aattgactgt
     1981 tattctcctc catttcttcg gcctttagta tgttctatgt ttctttttat atcaatcgct
     2041 tatccattca ttttgaatgc ttcatactac cctttgatta gctaaataca gccgtcaatt
     2101 gaggtacgac ttgtttttta cgtgaaacga cacctttaag caaagcacgg ttgttggtca
     2161 aagttgtttg gaaagcttct tctacttttt cttttccttt accagcaaca agtaattctg
     2221 aatcactgtc taagatgttt gtgacaacta atacgaacag atcgtagcca tttgctttca
     2281 tttcatcttc cattgctgct tctagctctg cttgtcgatc catcacttca ttaagatcca
     2341 ctgtattgac ttgagcgata cgaacgttgg aactgcccat tgggaaactt tttgcatcca
     2401 aatcaagaag aaccgcagct gatttatcac ttagatttgt tcctgctttc aacatttcaa
     2461 gtccataagt gtttagatcg acttcagcga ttgctgcaag ttcttttgca gctgcttcat
     2521 cttcaggcgt acatgttggt gatttgaaca acaatgtatc tgaaatgata gctgagagca
     2581 tgattcctgc gatttcttta ggaattgtta cttggttttc tttatatagt tttaaaataa
     2641 tcgtacttgt acatccaact ggttctgcac gatagtacaa tggattagct gtttcaaagt
     2701 ttgcaatacg atggtggtct actacagcta aaatatttac ttcgctgata tctgaaatac
     2761 tttgttggaa ttcattatga tccacaagca tgacatctgc attttctgct ttagctgact
     2821 ctactactcg tggtgcagat agctgaaagt ggtctaatgc gtattgtgtt tcttcattag
     2881 gtgtacctag agcaactgct tctgtttctt tacctaattc attttgcaag tgtgaaaacg
     2941 caatggctgc cccgattgca tctgtatctg gattttggtg accaaaaact aaaatttttg
     3001 acatgtcatt cgctcctttg tgatttctcc tatttaataa tagcaaaaaa acggaaagaa
     3061 gcaatactta tcttctttcc gatgcgacct atcgcgttaa atattttgta tactcttctg
     3121 tatgaagcaa tcgtttagca ttttcgacac gttcttttgt aggtggttcg atcccttcta
     3181 atggatattt caaaccaagt gtttcccact tgtattttcc catcgtatga taaggaagaa
     3241 tctctactct gtctacattt gacaaggttt gaataaagtt gtctagtcgg atcaaatatt
     3301 catcataatc acttcgctca ggaactagta catggcgaat ccaaaccggt ttgttgattt
     3361 ttgacaaata ttgagccatt tctaaaatat tttcattgtc ccaatgcgtt agttttttat
     3421 gctcttcatt atcgatgtgt ttgatgtcaa ataacagtaa atctgtatac ttcatcaatt
     3481 cttgaaaacg tgaaaagaaa ggttcttcat acgtaaaagg ttttccgcat gtatccaatg
     3541 tagtatgcat cttccgctct tttgcttttt taaatagttc gatcaaaaaa tcgatctgca
     3601 atagaggttc tccaccacta acagtaatac cacctttatt tccccagtaa ggtctgaagc
     3661 gttctgcttt atctaaaagt tcatctgctg tgtattcttt ccctccgcca atattccaag
     3721 tatctggatt gtgacaaaat tggcaacgca tgcggcatcc ttgcataaac acgacaaagc
     3781 gcagtccagg tccgtctact gaaccgaaag tttcgataga atgaacatag ccgattgttt
     3841 tttcttccat ttaagttcac atcttttctt tgatttcatt ttaagtacaa gttagctgtc
     3901 gaagcgaagc tttctgaaca aaatttttcg caattgccgc ttcgttatgt gtaaaattta
     3961 cagggacgga acatacaagt cctgcccctg taaatcgttt ctttcaaata agcatactga
     4021 ttattcgatt ttgatgcgat ggctattcaa cgtcctttcg acgctaagtc gaaaaacttt
     4081 tttcacgaac tttttacatt ctgtcgtgtg aagttctcat caagacatct aattgttgtt
     4141 ctcttgtcaa gtcacggaat ttaactgcat aaccagatac ccggatcgtc aagttcggat
     4201 atttcattgg attttccatt gcatcacgca ataaatcatt actgaataca ttgacattca
     4261 aatgataagc tccttttgag aagtatccat ctaagacatt acgcaagttt tcgattcttg
     4321 tatcttgttc ttttccaaga cctgaaggat tgattgtttg ggtatttgaa attccatcca
     4381 atgcacagcc gtaattcatt ttagctgttg agttcaggct agctaataac ccactttgtt
     4441 ccgcattgta acttggattt gctcctggcg caagtggttt tccagcttca cggccatcag
     4501 gagtatttcc tgtattttta ccgtaaacaa catttgaagt aatcgtcaat aatgaagttg
     4561 ttggttttga gttgcggtaa gttttatgac gtttgatttg tcccatgaag tattccaaga
     4621 tccatgaaac taattcatct gcacggtcat catcatttcc gtatttaggg aagtcgccat
     4681 ccacttcaaa atcaactgct attccttttt cattacggat tgcttttact tttgcatatt
     4741 tgatcgctga caagctgtct gccgcatgag aaatcccagc aatacctgta gcaaatgtgc
     4801 gttgcaagtc agaatccatc aaagccagct gagctgcttc ataagaatat ttatcatgca
     4861 tataatgaat gatgttcaac gtattgacat atagttctgc taaccaatcc atcatattca
     4921 tgaagttatg tttcacttct tcaaagtcta gtatttctga tgtgattgga cgatattctg
     4981 gtccaacttg ggcgccagtc acttcatcga ctccgccatt gatggcatat aatagtgttt
     5041 tagccaagtt agcacgagca ccaaagaatt gcatgtcttt tccgacaact gttcctgata
     5101 cacagcaagc aatcgcacaa tcatcagaac cccattgttc acgaagctga ttatcatttt
     5161 cgaattgaat cgaagaactt tcgatagcga ttctgctagc atatgttctg aatccttctg
     5221 gaaggtgttc agaatacaaa acagtcaagt ttggttctgg tgaaggtccc atatttgtta
     5281 atgtatgaag cagacggaaa tcattctttg taactaatga acgaccatct aatcccatac
     5341 ctgcaatgga taaagtagcc cagataggga atcctgagaa taattggtta tattcaggtg
     5401 tacgcgcgaa tttgaccata cgtaatttca ttactaagtg gtcgatcaat tcttgtgctt
     5461 ctttttctgt gattactcca ttatccaaat cacgttggat atagatatca aggaatgctg
     5521 agatacgtcc gattgacatc gcagcaccat tttgtgattt gattgctgct aaatatccga
     5581 agtatagcca ttggacagct tcttttgcat ttgcagctgg tttagaaata tcatagccat
     5641 atttcgcagc catttctttc atttgcgcta atgcacgata ttgttcagtg atttcttcac
     5701 gtaaacgaat cacttcttca ctcattgtat gataaccagt catatcatga tcttttttct
     5761 tttgttccat caaatagtcg attccataca atgccacacg acgatagtca ccgatgatac
     5821 gtccgcgacc gtaagcgtct ggaagccctg taatgatttt gtttttacga gcagcacgca
     5881 tttcagcagt ataagcgtca aatacacctt gattatgtgt tttgcgccag tcagtgaaaa
     5941 tatgtgtcaa ttcatcgttt ggtgtatagc cattactttt tagcgcattg ttagccatat
     6001 tgattccgcc gaacggcatg aatgcttgtt tcaaaggttt gtctgtctgt agaccgacga
     6061 ttttttccaa atctttatcc aaatagcctg catcgtgtga agtaatagtt gatacgattt
     6121 ctgtgtccat atcatagaca ccgttacgct cgaattggat atcatttaac ttttgcaatt
     6181 catcccataa tttttctgtt gcttctgttg ggccttccaa aaagctgtca tcaccgcgat
     6241 attcagtgta gttattttga ataaagtctc tagtgtctac gccttctttc catttggtac
     6301 ctttaaatcc attccattgc tccattgtat ggacctccta aatttctgtt ttatgtaaca
     6361 gtttttacgt gattagtata acacaggacc ctatctttgc aagggttttc ttgtttttta
     6421 tttaacaatt tttatgtttt atttacatat atttaaatca acgttgacat accaacattt
     6481 aagcattgtg aacactttca tctttttttg caaacgtata aggatttatt aaaaatgaat
     6541 gaaaaagaga cgccgcctgt attaatacag atggcgtctc ttcattaaaa tactacaaca
     6601 gttacgactc gatgaaaatc tcaggagcat cgataacttc gatgacttct ccaccttttt
     6661 tctcatccat cgcaaatgat ccattcgatg ttcgatcacc tatagggaaa tcagttgggt
     6721 caatcgttaa ctgctgccct ttttgattca tcaaggttat ttcttttgat cttccttccg
     6781 acatatagat aatacggtgc ggattttttt tcagctcacg catgaccgtc actccccgct
     6841 tcgcacgtga agtttggctg atttcttgag caagcatgcg tttaacagct cctcgctgag
     6901 taacgattac gatcggcgta tcaccttctg ctaacacaag cagcccgttg ataatcctat
     6961 ctccatcttt caaattcata gacttcactc cagctgcctt tgctccaaca acgggaacat
     7021 ctgttaaagg atagcgcaaa cccattcctt gatgcgttac taagaaaaca tctaagcctg
     7081 gctgatcttc tttcagatac acagcaatca gttcatcttc ttccgccttt agcttcatac
     7141 agctgagcgg acggctctta taggtgcgcc atggttcgaa ctctgtcatt cgtgtttgct
     7201 tgatcaatcc attcttgctg atgaatacaa atgttttctc tgcttgaatt ttttgatatg
     7261 ggaaaacagc taaaatcgat tcgtctacag acagattgga gattgtttga gaaatgtgct
     7321 caccagcatc tttccatttc aaatcgggta gttcatggac agggcggtag atcacatttg
     7381 ctttatttgt gatcagaaga acatggtcca atgtactcaa ttcacctgag tatagcaagt
     7441 agtcgccttc tcgcatcccg atttcttctg gtttggaagc actataagaa cgaaggcttg
     7501 ttcgcttcac atagccttct cgcgtcacac tgacaattac gtcttcttgt gccactaaga
     7561 cttgcgtatc aattttgatt tcttcgattt cgtcttcgat tgtcgttaga cgagcggaag
     7621 aataattttt tttcacttct cgcaattctt ttttcataac agagaaaagc tctttgtcat
     7681 ttgaaaggat tttattcaac tctgtgattt gcgcaatcaa gctttcggat tccttttgaa
     7741 gctctgtgat atccgtgttc gtcaaacggt ataattgcaa tgtgacaatt gcttctgctt
     7801 gttcttctgt aaattgaaag acgtcgacta aattcttttt ggcgtctttt ttgtctttac
     7861 tttcacgaat cgttacgatt acttcatcca gaatcgataa agctttcatc aatccttcta
     7921 cgatatgctg tcttttttga gctttggcta aatcgaattg actacgtttt gttatgactt
     7981 gctttcgatg ctcgatataa cttcttaaaa tgtctttcag tccaacttga tgcggagtca
     8041 tatggtcgat agcgaccata ttgaaattgt aattgatctg tagctcagtg tttttgaaca
     8101 aataattcaa aattccttga gcattggcgt cttttttcaa ttcgacgaca atttgcagac
     8161 ctgttctatc actttcatct cgtacttcag ctattccatc gattttctta ttcaaacgga
     8221 tctcatccat ttttttcacc aaggtcgctt tatttacttc atatgggatt tctgtgatca
     8281 cgatttgctg tttattaccc ttcattgcct ctatctttgt tttcgaacga agaataactt
     8341 ttcctcgccc tgtttcataa gcttttttga tttcatcttt tccttgcaag atccctcctg
     8401 ttggaaagtc aggtccagga atatattcca tcaatttttc aaggctcgct tgtggatgat
     8461 cgatcatata gattgtgcca tcaatcactt cagctaagtt atgagtcggg atttctgttg
     8521 catatccagc agatatacct gtcgatccat tgacgagaag gtttgggtat cgagctggaa
     8581 ggactgttgg ttctttttct gtatcatcaa aattccacac gagatcgact gtttcttttt
     8641 cgatatctgc tagcatctct ccacttaact tggataatct ggcttcagta taacgcatcg
     8701 cagcaggtgg atcaccgtcc atactcccgt tattcccgtg catttcgatc agtacttccc
     8761 gcaacttcca gtcttggctc atgcggacca tcgcttcata aatactgatg tcaccatgag
     8821 gatgataatt ccccatgata tttccgacag attttgctga tttacggaat cctttgtcat
     8881 acgtattgcc atctttgttc atggcaaata atatccggcg ttgcactggc ttcaatccat
     8941 cacggatatc aggcagtgca cgttcttgga taatatattt ggaataacgt ccgaagcggt
     9001 cacccatcac ttcttctaaa gttaattctt gtacttcttg tcgattttcc atcaggtgac
     9061 ctcctattct acttcaaata actgattgtt ttcattttta tctgctcgtt cttcatctaa
     9121 taaatcatta gaaactgaag gagagatttc agtatcctct tttttatcta agatactgcc
     9181 atcttcttcg agcgtaaatt gcacgtgatt ttcgatccat tttcttcgag gttctacttt
     9241 gtcacccatc agtgtggtta ctcgacgttc agcttgcgca gcatcatcga tacggacgcg
     9301 gatcaacgta cgggaagtcg gatccatcgt tgtttcccac aattgttccg cattcatctc
     9361 tccaagtcct ttatatcgct gcagcatata tcctttgccg acttttttaa tcattgctgc
     9421 caattcgtca tctgtccacg catattcaat gacttgcttc tttccttgtc ctttggatac
     9481 tttatataaa ggcggtaacg cgatatatac tttcccagct tcaatcagcg gtttcatata
     9541 acgataaaag aaagtaagca gtaatacttg aatatgggcg ccgtctgtat ccgcatcggt
     9601 catgatgatt actttgtcgt aattacagtc ttcgatagaa aactcaggac cgactcctgc
     9661 accaatcgta tagatcatcg tattgatttc ttcatttttc aagatatctt gcattttagc
     9721 tttttcggta ttgatgactt ttcctcgtaa aggtagaatt gcttggaact ttctgtctct
     9781 tccttgttta gcagatccgc cagcagaatc cccttcgact aaatacaatt catttttctt
     9841 agggtttcgg gattgtgcgg gtgtcaattt tcctgaaaga agagattcgc cttttttacg
     9901 cttcttccca tttcggcttt cttctctggc tttacgtgct gcttctctcg cttctctggc
     9961 tttgattgct ttacggacca acatctggct catttcgcta ttttcctgca aataaaagcc
    10021 catttgttca ctgatgacgt tatctactac ggtacgagca actggcgtac caagtttttc
    10081 ttttgtttgc ccttcaaatt gcagaagatt ttcaggtaca cggatagaaa gtacagcagc
    10141 taacccttct ctgaaatcac taccttccag attcttgtcc cgttctttca gcaaaccaac
    10201 ttttctcgca tattcattat aagatttcgt cattgccgct ttcattccag cttcatgggt
    10261 tcccccatct tttgtccgaa cgttattgac aaaagataag acattttctg aatacccgtc
    10321 attgtattga taagcgacct ctacttcgat accttctttt tctcccgaaa aataaacaac
    10381 tggtgtcaat gtatcttttt cttcattcag ataatcaaca aattctttga ttccttcttc
    10441 gtaatgaaaa atttccttga ctggtacttc tccacgaaga tcagaaagag agattttaac
    10501 gccttttaga agaaaggcag attctcttaa ccgttcggct aaaatttcat atgaaaattt
    10561 cgttgtactg aatattgtat cgtctggtaa aaaatggaca gtcgtcccgt ttgctttttt
    10621 tgtttttcct atctttttca acgtaccgtc aggtttaccg ccgtttttaa atttctgctg
    10681 atactctacg ccatctctaa cgatcgttac agttagccat ttcgataagg catttaccac
    10741 actggctcct acaccgtgca gtccaccaga agttttgtac cctccttgac caaactttcc
    10801 gccagcatgc agcacagtaa aaatcacttc aactgttggt attccggaag catgcattcc
    10861 tacaggcatc ccacgtccgc tatctgcaac agttatacta ttgtcttcat ggatggtcac
    10921 atcaatctca ctgccataac ctgataatgc ttcgtcaact gcattatcga cgatttcata
    10981 gactaaatga tgcaagcctc ggctgtcagt ggatccaatg tacatccctg gtctttttct
    11041 aactgcctct aatccttcca atacttggat ggacgagtca ttgtattcgt tgtttacttt
    11101 ttttgccaag gcaaacgctc cttcaataat actttgcggc aaaaccgcac cacgattatc
    11161 atactagaaa aggtcgtgaa aaaaaagagg gtagacgaga aatcaatttt aattatctcc
    11221 taaccgcttt ttcgacattc ccctctatcg atcatctctt ctttgtggtc atttttattt
    11281 ttggttttct ttcaacttac caagttcaat cttaatacaa cgatccatca cgatatctgt
    11341 acgtcctgct tttcttagca tctcagctgc ttctttgttt tcaatcccta attgtgccca
    11401 gaacacttta gcatcagttt tcaaaaaatc atctgctact tcaggtaaaa attcactccg
    11461 tctaaataca tctacgatat cgattggatc atcgatttct gtcaaattcg catagacttt
    11521 ctcgcctaga atttcttcac cagccaataa tggatttact ggtatgattt tatatccatt
    11581 gtcttgcaat acctgtgcaa ccatgtaact tgttcgttct ggtttattgc tcaagcctac
    11641 gacggcaata ttttttgctt cttccaaata gttgaaaatc gtttcttgtg ttggattttt
    11701 tacagacatt tctgccaccc cttgcttttc tttttattcc atctattata cttgtatctt
    11761 tcattttttc aattgttcct gttacctatt catgctaaaa taaaggatag ttttcaactt
    11821 gaaagagggt aaaaaatgaa attgatctta ttacttattt tagcatatct tttaggttct
    11881 attccttccg gtgtctggat tggcaaattg tttttcaaaa aagatatccg acaacacggg
    11941 agcggtaata ctggaacgac caatacattt cgtgtattag gcaaaacagc cgggattgtt
    12001 gtcctgctta tggatatttt aaaaggtaca ttggctactt gtttgccgat gatcttccac
    12061 ctttctggga ttaaccctct ttggtttggg gtatgcgcca ttcttggcca tacatttccg
    12121 atttttgctg ggttcaaagg aggaaaagct gttgcgacaa gtgcaggaat gcttttagga
    12181 tttaatcctg tattttttgt ttattcttgt atcatcttta tcgttagttt attttgtaca
    12241 agtatggtga gtttgagcag tatgatcagt gcagtattga tcactttatc tacgatcgtc
    12301 cttccttatg tagctcctgt gattttggct aagcccaatt ggttactgac gatcattgcc
    12361 tttttagttt cttcctttat cttttatcgg cataaagaca atatcaagcg tatcagaaac
    12421 catactgaaa gtcgaatccc gtttggtttg aatagcagca agaaaaaatc ctgatacttt
    12481 ttcattccaa agagataaaa cgggagtgtg acaaatcgat gtcacactcc cttattactt
    12541 acaggatagc ttttttacac accttggtaa acggtgttcc ctgctaactt ctcggtcaca
    12601 acctcttact tcactgtgat ggaatatttt gtcttgaaga tttcatgggc acctagttga
    12661 ttgatcccta ctttttccat caagtttcca ctactgttca ccgcgtcagc gattccgcac
    12721 cacggttcga tacaaacgaa aggcgcttct tttggataag gagaccagat ccctacataa
    12781 ggcatatctt tatagcttaa agttacgcta tgggaagatt tttcacttcg aatggcatac
    12841 gcatttagac cccttgtttc aaaaaccaag gcatcttgct caaaaatttc acgattcaaa
    12901 gccagattcg tatttgtctg acctaacgtc ttttgatcca tatcgatgaa tgggcctttc
    12961 aatggtaggc gtaatcttga tttcaatgga gaaaatgcca gatagaaatc ttcaaatgac
    13021 aatccttctt caagaggtac attgaaagca ggatgtccac cgatagaaaa gaacatttct
    13081 ttttcaccgg tatttgccac ttgataatga accgtgatgc cgtctcctcc taattcatag
    13141 ctgatcacta attcaaaatc aaatggatac ttttctaatg tctccggcgt actccttaag
    13201 atgaaactag ccatttcttg tccatgttca atgacttcaa aatccatatc ccgagcaaat
    13261 ccgtgttgcc ccatttgata tgttttccct tgataaacat actgatcatc ttttaaacgt
    13321 ccaacaaaag ggaataaaac cggtgcatgt ctgccccaat acgctggatc accttgccag
    13381 atatactcta aaccatgttc ttttgatttc agactgatca attccgcacc gtgttcagcg
    13441 atttcggcga tcagctggtc attttgaata ataacagtca ttgccattcc tccaacttcg
    13501 acttactttt taaaatggga aggctggaac aaacatttct atctgcccca gtccccttac
    13561 ttctaataac tgtgttttgc taactcgtct ttaaaacggg aattcaatac ttttaaataa
    13621 gttcccttca tgcctaacga acgagattca atgattccag ctgattccaa ttttcgtaaa
    13681 gcattgacga tcacagaacg agtgatgccg atactatcgg caatattaga agcagtcaga
    13741 cgaccttcat ctccatccaa tgcttcaaag atagcttgta cagccttcaa ttcgctataa
    13801 gataatgtgt tgattgccat ttggacagcc gttgcactcc gtacatctgc ttcgatgctt
    13861 cgagacttct gataaaggat ctgcataccg acaaccgtag ctccatattc tgctaagact
    13921 agatcgtcat ctgcaaacga ttgttccata cgagataaga taatcgtacc caaacgttcg
    13981 ccagctccaa agatcggaac gacagtcgtc agaccaaatg gatatttctc tcttgactct
    14041 accggaaatg cagtaatatc actagtaatt gggatattag cttcagtttt catcaactgt
    14101 gccactgtat cagtataaga ttcaggaaat tgtttctcaa caaacattgt tttgattcgt
    14161 tcattgttca catcgtgacg ttcattataa cctagtaata ctccgtcact tcctatgata
    14221 taggtgttgc tgtcaagaat accacctagt gtgacagcca ttttatcgta aggcaaatct
    14281 gcttgcatgt caaaagtatt cttttgctgc aaaagtgtgt ttatcttgcg ggttttcgtt
    14341 agcaagtctg tcattgtcgt cctccgattt tttataaaat atagcgactt aaatcttcat
    14401 ttgccgcaat tgagccaagt ttgtcatcca catatgcttc tgtgatcgtt atttctccca
    14461 tttgcatatc tggtgcttca aataatagat cttctagtag tttttccaaa atggtatgca
    14521 gtctgcgtgc gccgatatta tctgtgtcac ggttgacatc gaaagcgatt tgagcgatcc
    14581 gttcaattgc ttcttttgta aacgtaactg acacattttc tgtaccaatc aatgctacat
    14641 attgttttat taaagcatta ttcggttcag tcagaatttt cacaaaatct tcagctgtca
    14701 aatcatctaa ttctacccgt attgggaaac gcccttggag ttctggaatc aaatcactcg
    14761 gtttagataa atggaacgct cctgaagcaa tgaataagac atgatctgtt tggatgatcc
    14821 cgtattttgt atttacttgt gaaccttcga cgattggcaa tatgtctctt tggacgcctt
    14881 ctctggatac ttctccagat tgctgtgatt tagaagtgat cttatcgatt tcatcgataa
    14941 aaataattcc actttcttcc gctaatttga ttgcctcact atggatatca gcatccttga
    15001 ctaattttgc tgattcttcc ttgatcaata actctaaagc ttctttgact gttaccgtcc
    15061 gttccacttt tttcttcggt gtcaaagcac ctagagtttc atttaggtcg atccccattt
    15121 gctccaatcc attgttcatc attggtgcag gtttcttttg gtcttcaact tcaatggtta
    15181 cttcacgact atccagtaaa cccttttgta gttgttccaa gactgtacgg cgattgattc
    15241 gaatatcttc tgtcacttct tcttgcggtt cagcggtatt ttgctgacta gcattaaaaa
    15301 gattcatcat ttgttcaaat tgattagtgg attgtttctg ttcttttttg ataccaggta
    15361 ccaagatttt taccaaacgt ttttctgcat tttttttagc acttgtgtat acacggctgt
    15421 actgctcttt ttctacgatt tggatcgcat tttctactaa atcccgaacc attgattcta
    15481 catctcttcc gacataaccg acttcagtaa attttgttgc ttcgactttg acaaatggtg
    15541 cattgacgat ttttgccaaa cgtcgtgcga tttctgtttt accgacccct gtaggtccga
    15601 tcatcagcat attttttggt gtaatatctt gttgcatttt ttcatctaac tgcaaacggc
    15661 gatagcggtt acgcaaagca acggcaacag atttttttgc tgcgtgctgt cctacgatat
    15721 attcatctaa ttcacttacg atttgtcttg gtgtttgttt taattcatac atatctactc
    15781 acctcttaca gttcttctac gataatatta tgattagtaa agatacagat atctgcggca
    15841 atatttaacg cattttctgc aatttctttc gcactcatgt cgttttgagc atagtttttc
    15901 attgctcggg ctgcagccaa cgcaaaattc cctcctgatc cgatagctaa gataccatca
    15961 tctggtgtga taacttctcc tgtacctgaa acaagaagca tttctttatc attcattacg
    16021 atcagcatcg cttcaagttt ttgcatagat tgctgtgtcc gccattcttg agctaattcg
    16081 acagctgcgc gttgtagatt cccgttatat tcgtttagtt tgccttcaaa tttttcttct
    16141 aaagtaaacg catctgcaac acttccggcg aaacctacaa caacttctcc attgtagata
    16201 cgtcgtactt tctttgcagt gcctttcatg acaacagatt cacccattgt tacttggcca
    16261 tctccagcca tagcaaattt cccgtctttt tcgacggcac agatagtggt tgaatgaaat
    16321 ggtgattcga ccatgtttgt ttcctcctaa atttgttctt ttccttattt ttacgctctt
    16381 ggatggaaag aacgatagtt tttttgtaaa ctttcttttg ttacatgggc atagatctgg
    16441 gtagtcgata aattggcatg tcctagcaat tcttggactg tccgcaaatc tgccccatta
    16501 tttaataaat gagtagcaaa tgtatggcgc agcatgtgag gatggatatc actatccaaa
    16561 ctgctttttt tgatcatttg attcaagaca tattcaattc cagtaggcgt gattggtttt
    16621 ccatgatgat tcacaaaaac gatatcatgc tcttcgtgat attttgtcat caatacctgg
    16681 cggccttccc tgaaatattc ttgcagagca tcttgagcaa aagaacctag aggagcatat
    16741 ctctctttgt tccctttacc atggatcaac atgacctctg aatcaaaatc aatgtctgat
    16801 accgacaaac tgctacactc acttaaacgg atgcctgacc cgtaaaggac ttccagtaaa
    16861 gcgcggttcc tcaaatccaa aggactatct cctgctacgc tgtcaaaaag tgcttgcatt
    16921 tcattttcat aaaaaaaacg tggcagtctc aaatttttct ttttgagatg aacgtaagaa
    16981 aatggatttt cttttatcac ttcttctttg agaagatact gataaaacga acgtaggctg
    17041 gctatcttgc gactgataga atttctgcta tagctaggac gatgatcagc taatgcactc
    17101 aaatagacac gaaccgtaaa atattccgcc ttcagcagat cagcgtctcc agattcctcc
    17161 aaaaaatgtt tgaaatggag aatgtcttct tcatatgctt ctttcgtttt ttctgaatat
    17221 cctctttcga caatcaaata acgcaaaaat ttttgttccc acgtggcttc tgtcattttc
    17281 tcaccttcct tttgtcccag agaataattt agcacagcgt ttataaaaag acaaataaat
    17341 tgtcagaatt taaccaatat ttctaagttt gttcacaatt ggcgcaattc ctcacatttt
    17401 gtttgagaaa ttcttaagaa aaacgcaaga aaaagagcga gactatccgt ctcaccctct
    17461 tcatggagtt tccttactca tttgggattg cagttttttc taattctgtt tttattgcct
    17521 cattagcttt taaagctcgt tgtgcaattg cttcatatct tgcttttttg tctcgaatgc
    17581 gctctggcag ttcaggaaac agaccatagt tggcattcat cggttggaaa tgttttcctt
    17641 ccgcatgagt aatgtagtat gccatacttc caatagctgt ttcttgtgga aactcgactg
    17701 gttccatttc ttttgctaag cgtgcggcgt tgattccagc aagtagtccg ctcgctgcac
    17761 tttcaacata gccttctaca ccagtcatct gacctgcaaa gaacagatct tcgcgtttcg
    17821 ttgattggta agtcggtttc agcaattctg gcgaattcat aaagctgttt cggtgcatca
    17881 caccatagcg aacgaattca gcattttcta acccaggaat catccggaaa acacgttttt
    17941 gctcgcccca tttcaagtga gtctgaaagc ccacgatgtt gtataaagaa gctgcagcat
    18001 tgtcttgacg cagctggata acagcataag gacgtttgcc agttttagga tcttccaatc
    18061 caactggttt cataggtccg aaaagcatcg ttttgaatcc tcgctgtgcc attacttcaa
    18121 ttggcataca tccttcaaaa tatttttctt tctcaaaaga tttaagttct gccacttcgg
    18181 cattgattag tgcctcatgg aaagctttga attcctcttc tgtcatcgga cagttcaagt
    18241 aagctgcttc tcctttgtca tagcgggact tcaaatatac tttatccata tcgatcgtag
    18301 ctttgtctac gattggtgca gctgcatcat agaaataaaa accatctgaa ccattgaatg
    18361 ctttgatgct ttctgctaat ggatgagaag tcaaaggtcc tgttgctact acggtgattc
    18421 cttccggtat ttcagtaatt tcttcgttta tcactgttac taatggatgc tctttgactt
    18481 ttcgggtaat tgtttcagaa aaagaatcgc ggtcaacagc taaggcacca ccagctggca
    18541 ctgctgtttc atctgccgaa gtgattacta ccgaatccaa tcggcgcatt tcttctttca
    18601 ataaccctac tgcattagcc aaactgtttc ctcttaatga attggaacat actagttcag
    18661 caaaattact tgtgtgatgc gcctcagtcg atttgactgg acgcatttca tataaacgga
    18721 cagggactcc tgcttgtgcg atttgccaag cagcttcact tccggcaagc cctgctccaa
    18781 caacatttac cattttcaat gaaagaacct cttttctatt atttttggat gtcttcttcg
    18841 taatctccat ttacacagac tacttgttta ccgcctttga ctttcttctc gaccagatat
    18901 ccgtcacatt ttggacaatt tctgcccact ggtttatccc aagaagtgaa ttcacattct
    18961 gggaaacggg aacaaccata gaatagtctg tttttcttag attttcgttc aatgacttgt
    19021 ccttcatgac atactgggca agtcacaccg atttctttta cgattggttt cgtattccga
    19081 caatcaggaa aattactgca agcataaaac ttcccatatt tccctaattt tattaccatc
    19141 ggatgtccac agacatcaca atcgaatcct gctggttcat ctttgatttg aattttttcg
    19201 attttttctt ccgcattcgt cagttctttt tcaaacggtt tatagaaacg atcaaccact
    19261 ttgacccacg cttccgtgcc ttcttcaatt ttatccaaat ctccttccat ttctgctgtg
    19321 aaatggatat cgacgatttg agggaagaat tcacagatca gtgaattgac aatttctcct
    19381 agttcagtcg gctcaaatct tttttgtgtc aattttacat agtagcgtct ttggatcgta
    19441 tcaagtgttg gtgcataagt ggaaggtcgc cctaccccgt tttcttctag tgcacgaatc
    19501 aacgtagcct cactaaaacg tgctggtggt tgagtaaaat gttgttttgg ttcaatatca
    19561 acagattgga ctatatcgcc ttcttcaagt tctggaagaa tattttcttt ctcttctttg
    19621 ccgtcatctc taccttccac atacacttgc atgaatcctt tgaatttgat ttttgaaccg
    19681 ttcgcaatga agatgacacc attttgttct aatgtcactt tcattgtatc taaaatagct
    19741 ggtgtcatct gactagccac aaaacgtgac cagatcaatg tataaagttt caattgatct
    19801 ttatctaaat attgtttgag ttcatctggt gtacgaagca cactcgttgg acgcacagct
    19861 tcatgggcat cttgtgcgcc ttgagcattt ttgatctttc ggtgatcatg tctagaaaat
    19921 tcttttccat acgtatcttc aataaatgct gcggcctctg cttttgctgt atcggcaatc
    19981 cgggtagaat cagtacgcat gtatgtaatc aagccgactg ttccttgttt acccaaagtg
    20041 attccttcat ataattgctg tgctaccatc atggtttttc ttgttcgaaa gtttaactta
    20101 cgggcagctt cttgttgcag gctacttgta gtgaaaggat tcgcagggtt tcttttgcgc
    20161 tctttctttt ctactttttt aacttcgaag tctgttccat caattctagc agtgacttct
    20221 tttacacttt gtgcatctgg taatttcttt ttcttaccgt cgattcccca gaaatttgct
    20281 ttgaatttct tgcgagcttt tttaaaatta ccgtcgatgc tccaatattc ttctggaaca
    20341 aattctcgga tctctttttc acggtcaata ataattttta aagcgacaga ttggacacgt
    20401 cctgcactta accctttctt gaccttacgc cagagaatag gactgatcga ataaccaacg
    20461 attctgtcta aggtacgacg agcttgctgt gcatctacta aatctacatc gatcgttctt
    20521 ggttccttaa aagctgcttt gactgcgtct tttgtgattt cattgaagac cacccgattt
    20581 ttttctttca gatccaaccc aagtaggtac gctaaatgcc aagcaatcgc ttctccttct
    20641 ctatccgggt cacttgccaa gtaaactttt tgagcttttt ttgctgcggc tttaaggctt
    20701 ttgatgacat cgccttttcc gcgtatagaa atataatggg gttcataatt gttttcaaaa
    20761 tcgataccca ttttactttt cggtaaatct cttatatggc caacactggc cattacttta
    20821 tagtttcgtc caagatattt ctcaattgtc ttggcttttg caggtgattc tacaatcact
    20881 aaatatttat acgccattac tgaatggact cctttccatt gtcgtgttcc tactattaaa
    20941 agaggattca tacaaatcgt agctaatcat atccattcag aaaacgtttg tcaagaaaag
    21001 caaatgaaaa gtatggtgtt attctttaaa ttctgccaag atatcttcac ccgaaaccgt
    21061 gcaaactgca ccgtcttgga tcaaatgatg acatccggta gaatgttctg ttaaaatatt
    21121 tcctggaaca gaaaaaactg ttcttccaag atccaatgct tgctgtgctg ttatcagaga
    21181 gccgcttttt ttcttggctt caaagacaca tgttccttta gacaaagctg cgataatccg
    21241 atttctcatt ggaaaacgat gctttttcgg gccggattga ggaggatatt cactcaaaag
    21301 aagatgatca tttgcaatca cctcttgcaa gtcacgattt tcagcaggat agacttgctc
    21361 tatccctgtt ccaatgactg cgattgtccg tccaccatga gagattgctg cttgttgacc
    21421 gtatgtatct atccctttcg cgcagccact gacgatcaca tatccagctt ctaccactgg
    21481 ttttagtaaa taataaactg tttttttggc taaagcagaa gtgtgtcttc caccaacaac
    21541 gcttaacatg ttacaagaaa gtaaagatag atcaccatta taaaacagta caattggcgg
    21601 ataggtcaag tgaagtagct gagggggata tttgggatca aagcaagtga taaacgactg
    21661 tttcgattgg attttgcccc agttagcgtg aatttcctgc caacttttgc gaaacagctc
    21721 ccgatgtgtg gttattttac ctatcttaat gatgtcttca aatccgattt ctttataagg
    21781 atactgcatc atccagttag caagtgccca ttttccttga ctactgattc cttttgcata
    21841 agcaagtttc aataaattct cttctatttg atacatcata aaaccgcctt tcgtctctct
    21901 ttctaataag atacgtaaaa gcggctgttt tttattgtaa atattttttt acaggtgcaa
    21961 aacttttacg atggattggc gtaattcctt gtcgttcaag gccttctaag tgttcctttg
    22021 ttccataacc tgcattatgc gagaaaccat atccaggata aagcacgtca tattctttca
    22081 tcaattggtc tcgatatact ttagcaatga tacttgcagc agcaatcgac acagatcgtg
    22141 catccccttt gattattttt tcctgctgga taggcaaatc caaggtcatc gcatcgatca
    22201 acaaatagct tggtacaatg gacaattttt ctactgcttg gatcatcgct aatttacttg
    22261 cttcataaat attcacatca tcgattgtct gctgatcaac gatcccgatc ccgattgcca
    22321 gggctttttc ttgaacttgt tgataaagaa gttctcgctt tttctctgat aattgtttgg
    22381 agtcattcag ccctaatatg atctcatttt tcggcaagat aactgcagct gccacgacag
    22441 gtccagctaa tggtcctctt cctacttcat caatgccggc aatccattgg tgaccttgtg
    22501 cgtacccttg cttttcaaag caaaacattt cttcatattt ttccttaatt aatgcttcct
    22561 ttttcaatcg tcgttcaaaa gaagccagtg cttgctgcac gcctttgcgt ggatcatttc
    22621 ttaatgattc tagtgtagga tcatctattc tctgtatgct tttcaatcgt tctttgatac
    22681 tttggatgga ttcagtcatc acttttttgc tccatttctt cgcaacgatc cagtgtgtat
    22741 cgtccaagtt ttccttggcg aatatcatgc acgactaatt cgctgccgcg atcatagtct
    22801 tctttatacc cacgttttgc tgtaatatcc atcagtaatt ccggtgccaa tttttcttct
    22861 tgctcgattg ataaaccata acgatcgctg attcttcctg aataatatcg ggcaaaaaag
    22921 tctaatccgt aaatcgctaa atcatctaaa tggacaagct ggtctttgat cgcgcctgtc
    22981 agcgctagtt ttttgccgat ttcttcatct tcaaatttgg gccataaaat ccctggtgtg
    23041 tctaacaatt ccaactcatt acctaaacga agccactgct gaccttttgt tacgccaggc
    23101 ttatttcctg tctgtgcaat tttcttgcct actagtcgat tcatcaacgt agatttccca
    23161 acatttggaa tacctaagac cattgcccga atagcgcgag gcttgatccc ccgagcagca
    23221 tctcttgccc gtttttcttt caatgcttct ttagccgcag gtactagacc tttaattcct
    23281 ttactttctt gtgccgtgat agctaatgca agatggcctt gtttacgaaa atattcttgc
    23341 cacagctttg tctgttgtgg atctgccaga tccgctttat tcaataaaac aaggcgaggt
    23401 ttttgctgaa tgatctgatc tagcattgga ttacgagaag ataaaggtaa tcgtgcgtct
    23461 accaattcaa atacgatgtc cacatatttc attttttcag aaacttctcg tcgggctttt
    23521 gccatatgtc ccggaaacca ttggatggtc attgttctct ccattccttt cataccggaa
    23581 tattccccgg ctttacttgt tttttttctt tttcaatata aattcacttt tttattataa
    23641 agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc acatcttcac
    23701 ttttttgatg atgtaaattt ttattttggt ttctatatat gaatctaaat ttttgacaac
    23761 gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac aaatgaatag
    23821 tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt tttatgaaaa
    23881 gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa tggtatggga
    23941 tgacactttt tgggtaatgc ttctctctca tgatttttat aaaaaattta taaaagataa
    24001 agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg ttgaaaacgc
    24061 ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt tccacgaagt
    24121 agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc tagacggaaa
    24181 tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg ttttttataa
    24241 caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt ttcattcgta
    24301 caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa ttgttagatg
    24361 ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg tatcctttta
    24421 ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga cgtttttaga
    24481 ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc ccttgatgtc
    24541 atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg atttttaagt
    24601 atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga aatgttgtcg
    24661 aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt tcttatcacg
    24721 gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat aaacctgcct
    24781 tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat caattcttgg
    24841 ctgatgtctg ttttgtttgg tgcagatttc aaagtgattt ttctaggtag agtcatcagg
    24901 aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa aactttagag
    24961 gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta aactgatttt
    25021 ggattaaaca ataaagcgtg cagtctcaac agattgcacg ctttattgtt tctcactgga
    25081 tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga atatcagttt
    25141 tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct ccaagtacaa
    25201 aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta aagtccgtcg
    25261 tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt tccttaacta
    25321 ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct ggaagaccga
    25381 ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata tcaaatcgtc
    25441 tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg ttttccattg
    25501 aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac gcaaggaata
    25561 cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt tgtttttttg
    25621 tcaatctcta tcaatccatc cctcgttttt tcattctttc tttattataa gaaaaatatc
    25681 aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc tattggacaa
    25741 aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt attttctaaa
    25801 tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac attcagttcc
    25861 agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat gtctttaccg
    25921 gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag tgtcattaaa
    25981 taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata aaggcagccc
    26041 ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct tgatggtctt
    26101 cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc tgttcgaaac
    26161 gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt tcttctgtca
    26221 aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg aaagcgtcag
    26281 ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc aatgttggat
    26341 catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact tgttcataag
    26401 tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata ccaggaagcg
    26461 tgtcgaaagg ctggatcata caccagaaac agcctcctgc aaatattgct cgttccatta
    26521 tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt cttgctttca
    26581 gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga ggtatctttt
    26641 ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc tgctttaata
    26701 tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag atcatctgta
    26761 ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat cacatctttg
    26821 attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac atttccgttt
    26881 tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag tacttcgaaa
    26941 gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc actatctttt
    27001 tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt tgaattgatc
    27061 gttaagacag gatctccttg tctttccgta agagctggta tttctgggag ttctggttcc
    27121 cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac cccaagaagg
    27181 ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga tacgtcagat
    27241 cttgttttca tgtttattga tcatcctcct tctctagcca ttggttttgt gtctcgatca
    27301 gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga tggaaatgat
    27361 cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg ctagttgttt
    27421 gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa agcaaatcat
    27481 tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt cctttgttcc
    27541 aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa aatggattgt
    27601 agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct tccaatagtt
    27661 ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc gttaacccga
    27721 ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt gtgataaaat
    27781 cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt aagatctggt
    27841 cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa ttataacggt
    27901 cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga tctcctactc
    27961 cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta tttggatctt
    28021 ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa gcagctaaac
    28081 ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaga tgtcgtttcg
    28141 ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa aaattttatc
    28201 aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat aaagaaaaag
    28261 agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta atctacataa
    28321 tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt cgtatgcagt
    28381 acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc ttgcgccaat
    28441 tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc tttcagttca
    28501 tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt agttacaggg
    28561 attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact agacagcaaa
    28621 ccagttgttc gactgattct tccacctttt accaagttat ctagtgtgga tattccgatg
    28681 tataattttg tattgtcttg tacttttttg acttcttcaa ggatttcctc tactgtttta
    28741 ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg gtcagtggta
    28801 tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct tgctgcttct
    28861 actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc gtcttctcct
    28921 agctcatcat acatcgagat aaattctcca atcggtggtt ggcttgtttt gggcagattt
    28981 tttgcttttg ccatcatttc cataaatctt tcgccgtcta gttggtcatc atcgggatat
    29041 actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc atcgcgtaca
    29101 tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat tattttctct
    29161 ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac aatttctatt
    29221 tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag gagccgtttt
    29281 catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt ttaatgcagt
    29341 cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt tagtaaaagg
    29401 tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat cgatgatgat
    29461 tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag caaaaaaggt
    29521 gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt atactccttt
    29581 ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa tctggttgtt
    29641 tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga gagtatcaaa
    29701 agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg ctgttcctct
    29761 ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc tttcttacac
    29821 gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg tttggcattt
    29881 gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta cttaataaaa
    29941 aaaaataaag ctatggatgg gacagctcta aataacagct agtctatcca tagctttttt
    30001 atatcgtcaa ttcgcctgtc ccgcttttta caggattctc attacgctga tacgtttcaa
    30061 attgatgcgg ataacgattt ttttcatcta tttctccttg actagtattg atcagtgacc
    30121 aatcagacca gtcaacaggt ggaaaataag catctccatc gaatacatga tggatgactg
    30181 tacggtataa tatcgtacaa aatggcagaa attcttgata aacagctgac cctccagcga
    30241 tgaaaaagat accgtctgtt tctttttcca gagcaagcac gtcttccaca ctatgcatga
    30301 catgcacgcc ttctgcttta taggaagaat ctctcgtcaa aacaatcgtc tgacgattcg
    30361 gcaacggacg gctacccatc ccttcgaaag tttttcgtcc catcactagt gtatttgctt
    30421 ctgtcatttg tttgaagaat ttcagatcat ttggcaaacg ccaaggaaga cggtcttctt
    30481 ttccaatcaa tccgttttca tcctgtgccc aaattgcagc taacattttc tcatcctttc
    30541 tgattctaaa cagcgatagg agctttgatc gccggatgtg ggtcatatcc ttctaattgg
    30601 atatcttcca tctcaaaatc aaatactgac tgcttttcct gattcaaaac taaagtcgga
    30661 aatgcgcgta tttctcggct caattgttct ttcatttgtt cgatatgatt gatataaaga
    30721 tgagcgtctc ctaatgtgtg aacaaattcg ccaaccgcta aacctgtttc atgtgcaatt
    30781 aggtgagtca gcaacgcata acttgcaatg ttgaacggaa cgcctagaaa cacatcccca
    30841 ctgcgttgat acaattggca gcttagccgt ccctcttgaa cataaaattg aaacatcgta
    30901 tgacaaggag gcaaagccat gctaggaaca tcttccggat tccatgcaga aacgatcaat
    30961 cttctagaat caggagtctt tttgattgct tcgatcactt ctttgatttg atcgataaag
    31021 ccaccgtctt ttgtttccca gtgacgccat tgatagccgt agatgtgtcc aagatcgccg
    31081 tatgtttcag caaaggaatc atcggttagg atcttctcgc aaaaaagctt gctctgcttt
    31141 ttatattctt cattgaattc aggatctgtc aaacatctat ggccaaaatc agtcatatct
    31201 ggtccattat agtctggact tttgatgtag cgttcaaatg cccattcatc ccaaatatgg
    31261 tttttatttt gtaatagata gcggatattc gtatctcctt ttaaaaacca aagcaattcg
    31321 cttttgatca atccaaaagg tactcgtttc gttgttaaca aaggaaaccc tttttgcaga
    31381 tcaaaccgca tttggtatcc gaataagctt aatgtgcctg tgcctgttct gtcttcttta
    31441 cgatgccctt cttccaacaa ttttcttcct aagtcaagat acgcttgttc catccttttt
    31501 cctcctaatc ttctacccat tcactcaagt attcccaccg tgccattttt tcttcaagtt
    31561 gtgcttctgt ttcagatacg tcgttttgca attcttgtaa tcgagtgaaa ttatctcctt
    31621 gatgattcat ctcttcttgg agcaatgaga ttttttcttc aagttctgca atctcatctt
    31681 caatcgtttc ccattctttt tgttccatgt aagataattt cttcttttca gcaggttcct
    31741 tgctctgtat ttttggtttc gctttttcag caggctgctg ggtctctttt tgcttcagta
    31801 aataactgga catgtttccg taataaagtt cttgttttcc gtttccttgg aatactaata
    31861 acttatctgc taccttgtcc aagaaataac ggtcatggga aacagtgatc acagcacctg
    31921 gaaaacttct aaaataatct tccaaaatcg tcaatgttgc tatgtccaaa tcattggtcg
    31981 gttcatccaa taataaaaca ttaggttgct ggatcagcag tttcaacaaa aataaccggc
    32041 gtttttctcc gcctgaaagt ttgcctatca gtgtcccatg cataaatctt ggaaataaaa
    32101 atcgttcaag taattctgct actccgattt gactgccatc tgctgttttt gcttcttcag
    32161 cagcttcttg taagtaagca atcatccgtt tatctggtgc catttcttca ttttcttgtg
    32221 tataatacgc aagatgaacc gtttcaccga tagacattgt accgctatcc aggggaattc
    32281 tgccagctag aatattaagc aatgtcgatt ttcccgcacc atttttacca gtaataccta
    32341 gtctttcccg agattgaatc agtaaatcta aatgttctag cagtgtttgg tcatttatac
    32401 ggtaactgcc atctttgatt tccagtactt ttttccctaa acgttgtgtc gctaggttca
    32461 gctctaaatc atcttcttgg tttacttggt acaagttctc ctttagatct tcgaaacggt
    32521 tgatccgtgc ttgctgcttg gtgccacgtg cctgtacgcc agccctcatc caagctaatt
    32581 cctgtttata aagccgcttg cgcttttctt cttgttctac ttctacacga tcacgttctg
    32641 ctttttctaa gacgtaagct tcataatttc ctttatactc gtataatttt ccaaatgaaa
    32701 gttcgaagat tcggtttgct actctatcta gaaaataacg atcgtgggta accatcaata
    32761 gtgcgccacg gtattgtttc aaatagtttt ccaaccactc gatcgcttca taatctaaat
    32821 ggtttgttgg ttcgtctagt aatagtaaat cgggttcatc aatcaacact tgcgccagac
    32881 taactcgttt tttctgacca cctgataaag tactgatttt tttatctaat tcactgattc
    32941 ctaatttttg caaaataatc tttgcgttgg tatctgtagt ccaagcatct tctttattca
    33001 tttgttcttc tgctttagca taacgttttt gcacatcagg gtcttctccg ttttccccta
    33061 gttcgatcaa tgcttcctca tatgctcgaa cagtttggat caaggggctg tttccttgaa
    33121 agactgcttg gagaacagtt aattcatcgg aaaattcagc tgcttgagaa agataaccaa
    33181 tacggtaatc agttggataa aacactgttt gccgatcacc gtcgccgcta tcgataccgg
    33241 caataatatt taagagactt gttttacccg tcccgttcgt cccaatcaac ccgatacggt
    33301 ctttttcgtg gaccaaaaaa gaaatctggt caaacaaatc tttttcacca taagtttttt
    33361 tgaggtcagt tacttttaat tctttcaaca tgttccctcc ttatcttgga tattgtatca
    33421 taaaaatagt gaacttcagg aaaatttttt tggtatttgt tctttttgtg ttctcaaaaa
    33481 aagaacagga ccgattcgat cctgttctga tatgttttgt tttttataaa acttgaaaat
    33541 ttttaccttt ttattttatt ggactcgttt ttttgacttt gctcgaaacg caaatcggaa
    33601 agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga tattcctgcg
    33661 atagctgttc gcagaaaacg tcgtgatttt ctcagcaact tcgtattcat caatgatttt
    33721 tctttcaaga taataacctc ttagcatttt ttttgtaaac agactatctt gctggatcat
    33781 ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc gttgctggat
    33841 aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac tttcaataat
    33901 caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat cactaataaa
    33961 tgtctgacga tcagcatgtc ttctttgcca aaacattttc ggatggagaa cgattcgcat
    34021 taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc ttgtgataga
    34081 atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag aaagcttgcc
    34141 cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg cttgtatttc
    34201 ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac gtatccgttc
    34261 ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac gatcaatttc
    34321 ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag cattcaaatc
    34381 cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc ctatgaccaa
    34441 agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt aaaacatcat
    34501 attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc cctttacacc
    34561 gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac tgccatcttt
    34621 gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga cagagattaa
    34681 tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata ctggcgagaa
    34741 cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg taaaagcaat
    34801 agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag agattttcct
    34861 aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag atacatcaaa
    34921 tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca gataccctgt
    34981 gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga accagacaag
    35041 caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc ccgcgtttac
    35101 tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag taacaccaga
    35161 tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag ggatattcac
    35221 tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg ctccaaaagc
    35281 aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac ctaccccgac
    35341 aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat ttttgagaat
    35401 cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa tcataactaa
    35461 gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta ataccactcc
    35521 aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag ctatgttcga
    35581 taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg tccatcccct
    35641 ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta aaattttggt
    35701 ttcgactact tcaacctgtg tttggttacc ctctaattca cactgtacac gttttgactc
    35761 caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata tatttctttt
    35821 acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat agcgatcatg
    35881 caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct atacacaaac
    35941 ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt catcgtttat
    36001 tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa tagtatcaga
    36061 tatcaagtaa aacttaagaa agataaaagt ttctgacaaa agaatgttta cttttccatt
    36121 cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa aatcttgtca
    36181 cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt aataagacac
    36241 atctctttca cagcttcatt tttgcgtaaa aatcgattta ttcgaaaaat tccccgtata
    36301 catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt ttttcgctga
    36361 tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga tatgggaagt
    36421 acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata tcttcgtatt
    36481 tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca tgtgtcgcaa
    36541 ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg atttcaacaa
    36601 attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa agatcgccga
    36661 tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt agcgctgtca
    36721 tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac tacctcagtt
    36781 tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga cgtatctgcc
    36841 cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa atcaattttt
    36901 ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt tctacgacgg
    36961 ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt ccagtttctt
    37021 ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt aaacgatcat
    37081 aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc aattcttcta
    37141 cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa gctccagttt
    37201 ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg ttagaacact
    37261 tccacgattt caaaaaggaa cgaatctcat tttcttttag acccagttgg ctgatcaata
    37321 atgtccatac ttggctctct tctttcaatg ggacggatgg taattcagca aaacgtaaaa
    37381 gtgcttcgcc ttcttctttc aaactgggac agcaaatgta gcactctgtt tcaataaatg
    37441 catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc acatttatac
    37501 gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag aaggtttctt
    37561 cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt cgtaaggcat
    37621 cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct tctaaatctt
    37681 gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac gcattgatcg
    37741 taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca tgatctggtc
    37801 tgcggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat tgttcatcat
    37861 tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt ggaaaaagct
    37921 gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt ggctggttca
    37981 gcaagatatc acgtacactt ccacctacaa aataagcttc atatcctgca ttctctaatt
    38041 ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat ttcatttttc
    38101 caacctttcc tgcgtgtttc ttgtactgag ataaagactc agttgcatgg tgactgtttg
    38161 acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac tcgtccttac
    38221 gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca aaaacttcag
    38281 ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca tcaccaagtt
    38341 cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca taagtgtggt
    38401 tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga gctaatggag
    38461 agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc atactgtata
    38521 aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac tttaacgcaa
    38581 atcctagata agagcagttg agaaaatcga atttccatgc tacatttaga aaggaatttc
    38641 taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg tcaaacgaaa
    38701 aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat ttggtcttgt
    38761 ttacttgaac atcgctaatc atttagcaga agggggcgtc tcagggatca ccttgatctt
    38821 aagagcttta ttcggaattg atccagctta tacgacttta ttaatcaaca tcccattgat
    38881 tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatacagttc taggtacact
    38941 ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca atttacagca
    39001 tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa gcgggatcgt
    39061 ctaccgcatg ggtggaacaa caggtggcag cgatattatt gctcggattt tagaacaaaa
    39121 ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct tacttgcttc
    39181 attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt atgttttcag
    39241 tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac ttgtcgtctc
    39301 caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag gtgtgacttt
    39361 tctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata tggtcgttag
    39421 ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa aagctttcct
    39481 ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga agccacagac
    39541 aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga aagattc
//
LOCUS       DAZKCZ010000011        38119 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000011 DAZKCZ010000000
VERSION     DAZKCZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.011"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..3322
                     /locus_tag="ACG8KN_000521"
     CDS             44..3322
                     /locus_tag="ACG8KN_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFP9187604.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3863..5770)
                     /locus_tag="ACG8KN_000522"
     CDS             complement(3863..5770)
                     /locus_tag="ACG8KN_000522"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFP9187605.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5900..6775
                     /locus_tag="ACG8KN_000523"
     CDS             5900..6775
                     /locus_tag="ACG8KN_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFP9187606.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6855..7829
                     /locus_tag="ACG8KN_000524"
     CDS             6855..7829
                     /locus_tag="ACG8KN_000524"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9187607.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8009..9154
                     /locus_tag="ACG8KN_000525"
     CDS             8009..9154
                     /locus_tag="ACG8KN_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9187608.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9291..9635
                     /locus_tag="ACG8KN_000526"
     CDS             9291..9635
                     /locus_tag="ACG8KN_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFP9187609.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9906..11192
                     /gene="efmA"
                     /locus_tag="ACG8KN_000527"
     CDS             9906..11192
                     /gene="efmA"
                     /locus_tag="ACG8KN_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFP9187610.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11330..11929)
                     /locus_tag="ACG8KN_000528"
     CDS             complement(11330..11929)
                     /locus_tag="ACG8KN_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9187611.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12333..13454
                     /gene="mnmA"
                     /locus_tag="ACG8KN_000529"
     CDS             12333..13454
                     /gene="mnmA"
                     /locus_tag="ACG8KN_000529"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9187612.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13700..15121
                     /gene="arcD"
                     /locus_tag="ACG8KN_000530"
     CDS             13700..15121
                     /gene="arcD"
                     /locus_tag="ACG8KN_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFP9187613.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15135..16253
                     /locus_tag="ACG8KN_000531"
     CDS             15135..16253
                     /locus_tag="ACG8KN_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFP9187614.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16313..17605)
                     /locus_tag="ACG8KN_000532"
     CDS             complement(16313..17605)
                     /locus_tag="ACG8KN_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9187615.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17755..18315
                     /locus_tag="ACG8KN_000533"
     CDS             17755..18315
                     /locus_tag="ACG8KN_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9187616.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18444..18821
                     /locus_tag="ACG8KN_000534"
     CDS             18444..18821
                     /locus_tag="ACG8KN_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFP9187617.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18754..20826
                     /locus_tag="ACG8KN_000535"
     CDS             18754..20826
                     /locus_tag="ACG8KN_000535"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9187618.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21037..21984)
                     /locus_tag="ACG8KN_000536"
     CDS             complement(21037..21984)
                     /locus_tag="ACG8KN_000536"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9187619.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22230..22694
                     /locus_tag="ACG8KN_000537"
     CDS             22230..22694
                     /locus_tag="ACG8KN_000537"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9187620.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22756..23799)
                     /locus_tag="ACG8KN_000538"
     CDS             complement(22756..23799)
                     /locus_tag="ACG8KN_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFP9187621.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24176..25141)
                     /locus_tag="ACG8KN_000539"
     CDS             complement(24176..25141)
                     /locus_tag="ACG8KN_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFP9187622.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFTLSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25375..26220
                     /locus_tag="ACG8KN_000540"
     CDS             25375..26220
                     /locus_tag="ACG8KN_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9187623.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26411..27598
                     /locus_tag="ACG8KN_000541"
     CDS             26411..27598
                     /locus_tag="ACG8KN_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9187624.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27604..27960
                     /locus_tag="ACG8KN_000542"
     CDS             27604..27960
                     /locus_tag="ACG8KN_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFP9187625.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27962..28300
                     /locus_tag="ACG8KN_000543"
     CDS             27962..28300
                     /locus_tag="ACG8KN_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFP9187626.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28376..28632
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28695..29729
                     /locus_tag="ACG8KN_000544"
     CDS             28695..29729
                     /locus_tag="ACG8KN_000544"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9187627.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29722..30408
                     /locus_tag="ACG8KN_000545"
     CDS             29722..30408
                     /locus_tag="ACG8KN_000545"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9187628.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30432..31280
                     /locus_tag="ACG8KN_000546"
     CDS             30432..31280
                     /locus_tag="ACG8KN_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9187629.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31477..32250
                     /gene="sufC"
                     /locus_tag="ACG8KN_000547"
     CDS             31477..32250
                     /gene="sufC"
                     /locus_tag="ACG8KN_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9187630.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32263..33549
                     /gene="sufD"
                     /locus_tag="ACG8KN_000548"
     CDS             32263..33549
                     /gene="sufD"
                     /locus_tag="ACG8KN_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9187631.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33546..34781
                     /locus_tag="ACG8KN_000549"
     CDS             33546..34781
                     /locus_tag="ACG8KN_000549"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9187632.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34768..35238
                     /gene="sufU"
                     /locus_tag="ACG8KN_000550"
     CDS             34768..35238
                     /gene="sufU"
                     /locus_tag="ACG8KN_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFP9187633.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35243..36634
                     /gene="sufB"
                     /locus_tag="ACG8KN_000551"
     CDS             35243..36634
                     /gene="sufB"
                     /locus_tag="ACG8KN_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9187634.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36730..37872)
                     /locus_tag="ACG8KN_000552"
     CDS             complement(36730..37872)
                     /locus_tag="ACG8KN_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9187635.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWIAHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNDLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGNVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(37911..>38119)
                     /locus_tag="ACG8KN_000553"
     CDS             complement(37911..>38119)
                     /locus_tag="ACG8KN_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187636.1"
                     /translation="NNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDNIN
                     KLEQTISSIGKKYTKKKMKGGDTI"
BASE COUNT        12366 a         7040 c         8094 g        10619 t
ORIGIN      
        1 actttaagta aacgctttct aatttgttac gggaggaata cacttgttaa aaaaacgtat
       61 aaaatacagt gcgctaatga tgaatctcct tatgctcagt accccggttt taatttctgt
      121 cgttgaagtt gctgctaacg aacaacagtc tgtgaacgaa gaaaatgcac acgtgaacat
      181 tgaagggatg tcaacaatgg aggaaatacc aagaactagt gaagatatgg atacggaaga
      241 aggaaatgat cctatgatga aagaatcaga aaatgaaaag acaaaaaagc aatctgaaga
      301 agcaaatgaa gagtggaaag gggttgtttt cggagaaagc acaagtgcta gtaggtatgc
      361 cattgaacca ttagaagacg gcgtgcggct ctcttccacg aataacggcg gaaaattcca
      421 gtcaagcgga tcagatggaa tgacctatta ttatcaagca atccccaaag acatcaactt
      481 taggatgcgg gcgacgatcg aaatagaatc ttggacctat acgaatgccc aagaaggatt
      541 tgctttaatg gtaagagacg cagtaccaaa aaatcatttg tttgggcagg cattttattc
      601 aaattcattt gccatactag gcagtcggat cgaatatgtc tgggactcgg atagacaaga
      661 agtggtcaac acgagcggca gcaaatatac gatgcgactt ggattgggga cacgggcaat
      721 cacaggaata tcatctgaag atccgcaagc tgcgccagct cctgggagtg tctcagtgga
      781 aacaacacct ttagagactt cagcagggtt cttggaaaaa gaaacaggtt cttataatat
      841 attcggaaac ggtcatggga atctgtttcc ggccacgaac tcgattacta aactggacgt
      901 agagatgaaa aaaacgaata ctggtttaga ggcatattat tacgatcctg aaacaggaga
      961 agaaatggct tctgacatca tgtatgactg ggaaaaatta tacgcttcag atgaatcggt
     1021 tatttatgca ggatttgctg cagcaaggaa tatgacgatc aaggtagaag gaatcgaagt
     1081 tgcttactca gatcctgcga cagatcctcc tgggcaagaa agacctactc gcttgattga
     1141 tccgatttac actgtgacct ctagtaatca ttcaggaaac caagatcata ctatgtcctt
     1201 tagagcaaat gctgacggat tattgacaat taaaaataaa gcaacaggag aggtgcttaa
     1261 gaacgcaaaa aacttggcta tcactgcgaa tcgggaattt gattatcaga caaaactgat
     1321 ggaaggaacg aacgaattta ctttcagttt tacgccagat aaaaactatc cgccagaaga
     1381 atacgtagag atgactagtt atgaaacaaa agagattctt catacagtgg actaccgtaa
     1441 acctaaatat tcgacagttt atgtaacacc agaagggtca gatgcaggaa acggaagcag
     1501 acaaaatcca ttatctctga cacaagcatt gagatacgca tatgctgggc agacaatcgt
     1561 catggcgcct ggaacttatt ccttcgaaag aggattgacg attccaaaag gcgttaacgg
     1621 gacaagagaa aacccgattc agttgattgc agagaaaaat ccagaaaaca agcgtcctgt
     1681 acttgatttt aaaggaactg gaaatgggat gacactgttc agtcactact ggggtttacg
     1741 agggtttgac attacaaata gtgcagctat gcaaaaagga ttacagattt ctggaaatca
     1801 taatttgatc gaagagattc atgcttatcg gaatggaaat acaggaatcc aaatcagcgg
     1861 tagctcaaat gatcctttcg aagcatggcc cgcgcataac ttagtcaaga actgtacatc
     1921 ttttgaaaat gccgatccag gatttgaaga tgccgatgga tttgcggcca aactgacaat
     1981 aggagaagga aatgtatttg acggttgtat cgcgtatcat aatgcagatg acggttggga
     2041 cctttttgct aaaaatgaaa caggttcgat cggaaaagta atcatcaaaa actcagttgc
     2101 ttatcgaaac ggctgggtac caggaattga gggagaaggt aacggtaatg gattcaaaat
     2161 gggcggttct tctttaacgg gccctcatga attgatcaat agtttgtctt tcgaaaatct
     2221 tgcgaaagga atcgattcca atagcggaac agatattatc gtcaatagca gtacaagttt
     2281 taataacggt tcatataatg tggctcttta tacctcttca gcaggaaaca cagactacca
     2341 tgcttctggt atcttatctt tccgaacaga gaatcttgag atgagagagc agatccgccc
     2401 attgaatcaa aacgaagatc aaatctacca ctcgttgaac tactattgga atgaacgaga
     2461 gaaacaatca gaaaatactc ttggacatac catatcaaaa gattggatcg agtctctaaa
     2521 tacatgttcg aaagaaggtc agcagcctgt tacgcgctat gaaaatggaa gtatccatgt
     2581 tcatggactg atgcagatta aaccggggaa tcgtcaaaca gaaaaggaaa gagtggatgg
     2641 tcttccatta tctgtaggtg ctatttttga cggagaaacc tcaccgtctt cggaatatcc
     2701 agagtatgca atcgtacaag aaccaaatca gacaaaacca aatgaacaaa ataatccgat
     2761 tattatacaa gaagcaaatg gtgtggcgga gaatgcagaa ggaaatatca ttgaactgaa
     2821 gccttttaca gtttttgtgt caactttcac accaaatcaa ggaacgcctg tctttgaaga
     2881 tatgcgttat ttcccagaaa agatccaaat cgttgaaaca cagtcaagtg ctcaattttc
     2941 taatcatacc gctatttttt cagcaccttc aaaaggagaa tatacgctag aagtcactta
     3001 tcgttcggaa atatttgatg gggaaaaatg gtctgctcat acagaatctg ttacccagca
     3061 aaaagcgatc actgtgcgag aaactgccga tgtgcccccg ggaaatgaca aaccagaaag
     3121 agaaaatcca ggaaatgaat caccaaatgg aaaaccaaat ggaaaacctt ctcatccgga
     3181 taggacagca aaagaaagtg aaaaaaatgg cactcaagga ctcccaaaaa cgggagaatt
     3241 taataacccg ttgctagcat tagctggagg aattttgtta atcggtgtag ttgtttatgt
     3301 catgaaacag cgaaaaaaat aagtggaatt tctcctttta tgatgtaaaa caattgactg
     3361 aggaaagtaa aaatagaagg aaatgagacc aatagatgtc tcacttcctt ctatcttttt
     3421 atagatgagg ttctccttgc tgcttttgat tggctgttct tattacttga tgcaggacag
     3481 ctttttcaca atctctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa
     3541 caaacggcaa aatatgagga actatttttc cgtttttttc tctaatactc aggtcaggac
     3601 gctgatttca caacctctcg ataaacggtg ttcaaaagcc agtttctcgg tagtaagtca
     3661 aatttatgca aaaatgtgga aagccatttt cacaaaattt ttcttactac ttgaaactaa
     3721 gcagcttttt cacaacctct aatacatggt attccagaag taacttcttc gaaaaataag
     3781 ccgaaatgtc acaaaaattt gagaagcgat tttcccaaat tccttcttat ttcttgaagc
     3841 tgaccacttc tggcacaacc tcttaccaga ttcgcactct tttttctggc gctagataca
     3901 tagcgtcttg ttccgtgata ccaaatgttt catagaattc gtcaaggttt tttggttgca
     3961 cgtttgctcg gagtttagca ggggcatgga catcgatttg cagtaaaagt tgttggtatt
     4021 ctttttttgc ttttgtacgc caaatcgttg cccaattgac aaagaattcc tcgatagaaa
     4081 gatctgattc cgtttttgct gcttccaatg cacagcttag accgcctgca tcagcaatat
     4141 tttccgaaac ggttaatttt ccgtttactt ttccgtcagc aaaaggcaga ccatcgaatt
     4201 cttcgatcat tgcttgtgct ttgttttgaa attgtgtaag atcttcttct gtccaccagt
     4261 tgttcaagtt gccatattcg tcaaataaag cgccgttatt atcaaatgca tgggaaatct
     4321 catgagcgat aacagctccg attccaccat aattagcaga gcttgattgt tctagactgt
     4381 aaaatggtgc ttgaaggatc gctgccggaa agacgatcac attccgaaat ggatgataat
     4441 aagcattgac cgtgtcagca ctcatttccc attctgaacg atccactggt ttgttccatt
     4501 tgctgaaacg atcttttaat gtgagtttgc tgaaaacaag agtatttgaa agcaaagtac
     4561 caccctgatc tgcaggtact gttttgaact ggtcgaataa tgctgggatc ttatcggggt
     4621 agcctacttg gatacctaag ttatttaatt ttatgacggc tttttctttt gttgcatccc
     4681 caagccagtc attttcttct agacgttttt tataaacggc gatcattttt ttcaccattt
     4741 gttctacgtc ttttttagca gcttcgccaa aatatttttt tccgtagtaa tcgccaacta
     4801 cttgatcgaa ttgtccagag gcaaggtaat acgctgcttt tttctgaggc attgcttcgt
     4861 ctgttccaga caatacgcga gaatagattc cgctgacttg gcggaattct tctgatagat
     4921 aaccactaag agaacgtact gttttaatga tcatccagct tttgaataac ggaaaatgag
     4981 cttctgtcag gatgtcattc aaatgatcga agtacactgg atcagttacg atcactttat
     5041 ctggagttgt cccaatcaaa cccgtgacaa gttttttcaa gtccagttta tctgtgtatt
     5101 taataaatgt atcaaaagat tgcggattat acattttgct gtaatctgcg ttttcttctg
     5161 cactttttac atgaggagca atcaatttat caaattgcac tgcttgttcc gtgattgctt
     5221 ctgcctgact tttttctttg ccggcaagct ctaataactg aacagccata tcaaagaaaa
     5281 cgcctattag ttgtgcaccg ttgggatggt ctgatgcata atacgtctta tctggaagga
     5341 ataaagaagg cggataagca aagaaggcgt tcgtttgcgc attcttcata tctgcatcta
     5401 catccagatt aaatggcaat ggtaatgtat ccaacagcca ttctggaaat tgcttattca
     5461 attcgtcgaa agactgcaat gattcaactt tttcgattat aggttgaagc ggttgagcat
     5521 gcaactcatt tcttcggtca taatcgtttg ccagttgata atatgcaagg aaatgtttca
     5581 ttttttctga agacactttt tctggatggg caagcagatc gtctgtttct cccatcaaaa
     5641 gttggtcgat cccgtcaact aaatcttgga aaccgcctgt agctggttta tctgcaggga
     5701 tttttgctgt tttttgccat tcttcattga ctgcttcata gaaatcttgt ttcaatcgtt
     5761 ctttgttcat tcatcgatcg ctcctttttt gtttcgtgtg cccattatct tacaaaatga
     5821 aggaattaac aaatagaaca taaaaaattg aaaattcgaa caatcaaact gatttgtttt
     5881 tttcaatcct tcacttataa tgaaaaaaag gaggcgatcc caactgaatc aagtagtgga
     5941 taaaccttta tatttcaaag tagataaaaa aattaaaaaa ttagccagca ctcagcagct
     6001 gcagtcaaga aaaagtaaat tgcttttttt agctctggtt tttgaagatc aaagttatgt
     6061 gattatcgat caatcaggac atccggtaga atattctcca gcaaaatata cttatcaaga
     6121 gggtctttct tgtaaaaaat ggaagctgat caatgaagag ccaatcgagt tatctagatg
     6181 gatcaacaga aaagaagata ttcccgtttt gatcgaagaa aaaaggtcag gaaaggaact
     6241 cgcaaattgc tgggtgggat tgccagaaga acgttttttg aggtataaga aatgggctac
     6301 cccctcaggc tatctttgtg gtacttacgc ggcagcagtt ctccttgcct attatcaaga
     6361 ctatcgtaag gggtggatgc ttccaaatga gattcgcaag aaaaatacgg ctgattcgtt
     6421 ggttttgacg aaggcattgc gtagccagat ccaaccacta ggacttccta cgattccttt
     6481 tcaggttagt acagggattt ctagtttttt aaagaagaat ggcaatcatg agcgtgcacg
     6541 agcaactctt ctaggttcat ggcagcgagc tacaaaacgt atacgtgaag gcaaacctgt
     6601 gatgattggt attttgaaag tacttggcag tacatatggc aatcattggg tgacagcgta
     6661 cgcttatttt gaaactgaaa caggagaacg ttattacaaa gtacacgaca actggggcga
     6721 ttatcataaa gtcattcctg ccagttggag taacggaaca gtttcacttc cttaagaatt
     6781 tgctgaaatt aacagaaaat cttggaaaaa tatgttaaac tatcgaagaa tagagaagtt
     6841 ttggggagga ctacatgagt aataagtatc aggatgacac gttgaagatt tttagtttga
     6901 atggcaaccg tccattagct gaaaaaatcg cgaaagtgtt cggaacagaa ttaggtaaaa
     6961 gcgtcgtgaa gcaatttagt gatggcgaga tttccatcaa tatcgaagaa agtattcgag
     7021 gggatcatgt gtatatcgtt caatcaacga atgcacctgt caatgattac tatatggaac
     7081 tactgatcat gatcgatgca atgaagcgag caagtgcgaa aacgatcaat gtcgtattgc
     7141 cttactatgg gtatgctagg caagaccgca cggcgaaacc tcatgaaccg atcactgcga
     7201 aattgatcgc taacttgatc gaagaagcag gggcaactcg agtactgact ttagatctgc
     7261 acacggttca agtacaagga ttctttgata ttcctgtaga taatttattt acgatgccgt
     7321 tgtttgctca ttattaccgt caattaggtc tgacgggaga tgacatcgta gttgtttcgc
     7381 caaaaaacag cggtgttcag cgtgcacgta gtctttcaga atatttgaac agcacattag
     7441 ccatcgtaga tcatgcagat gatgcttcaa ctgagggtgg ttatgtgatc ggtgatgttc
     7501 aaggaaagac atgtatcatg gtagatgata tcttgaacac aggagcaacg cttgcacgag
     7561 cagcaaatgt attaaaagaa aacggcgcaa aagaagtata tgcctgtgcg tcacatggat
     7621 tattgtccga tcatgcgaaa agtattttag acaatgcacc aatcaaagat atttgtatca
     7681 ctgattctgt tttcacagaa gaaaatcgtc atccagaaaa cttaacaatc gttacatgtt
     7741 cagaattaat gggtgaagcg gtcaaacgca tccatgaaaa tacaccgatg agtccgttat
     7801 tccgtctgga agaaaaagaa ttcgaataaa caaaaaaaag agggaaagaa gttgtgacag
     7861 aagtgagcag cttcaaggag ctgcgacaag acttttgtca cagctccttt tttctatgcc
     7921 catgaaacgt cctctagaca aaaatgagct cttctttggt ttgttcgcta ttttatgcta
     7981 gaatgtgcct agcaataatt tggaagggat gaatgaagtg aaaactattt atctggatca
     8041 tgcggcaact acccccatgc atccacaagt aattgaagcg atgacggcaa tcatgcaaga
     8101 cacgtttgga aatccttcaa gcatacatgg atttggtcgc cacgcgcatg aaaaattgga
     8161 aacagcaaga caaatcattg cagacagtct gcaagcaaaa cctcacgaga tcatctttaa
     8221 cagtggggga acagaagggg ataatacagc tatcttagaa acagcgttct cgcgtcaaaa
     8281 agaaggcaga catctaatca ctacagctat cgaacatcct gctgttctta ataccatgaa
     8341 atatttagag acaaaggggt ttgacgttac atatctgcct gtagatgaaa aagggaatct
     8401 gtcgatcgaa gaggtcaaaa aagcgttacg agaagatacg atcttggtct ctgtgatgta
     8461 cgggaacaat gaaatcggaa atttgatgcc aatcaaagaa atcggtgaac ttttagcgga
     8521 tcatccagct tatttccata cagatgcggt acaagcgtac ggaaaccaaa cgatccatcc
     8581 agaagaatta ggaatcgatt tattaagtat ctctgctcat aaaatcaatg ggccaaaagg
     8641 cgtcggtttc ttatataaac gagacggagt aaatatccct gcattgcttt taggcgggga
     8701 gcaggaagaa aagcggcgtg caggtacaga aaatttagca gggatctggg gtatggcaaa
     8761 agcagttgaa ctgctgtcac ctgaagaacg tgaagcacgt aacaacaaat ataaaggatt
     8821 ccagcaattg attcttgata cattagaagc aaacgaaatc ccttatcaag taaatggtga
     8881 tttaacgaat aaactccctc atgtgttgaa tcttcgctta attggcgaag caaatgattt
     8941 gctactgatg aagcttgatc ttaatggggt cgctatttct actggttcag cttgtacagc
     9001 aggaaatatc gagccttcac acgtattaga agcaatgtat ggagaagata caccgatact
     9061 aaaagaatcc atccgtatca gctttggttt gggaaatacc gaagacgaca tccagtattt
     9121 ccttgatcaa ttagttcgtg ctgtcaaaaa ataactagtg aagtcattct tatatgaaca
     9181 gatgtctgag agaacgcctt cattttttag ggaacttaac tgaaaaacct agtaaaaatc
     9241 aggataaact tttataataa agagaaacta attttatcga ggtgaaaatc atggcatttg
     9301 aaacaacagc aacggtactg ggcgcaccaa ttagctatcg tttgcatcct gatgcaaaaa
     9361 gatatacatt gcgcgataat ggatttacag aaacaaacgg aggcaattat caattgatcc
     9421 gaccattaga tgccacgccg caaagcaaag aaggattcaa attaaaaatc actgtatcaa
     9481 aagatattca aacattgaaa atgtcgatca ctacagcaaa tggattgaaa gcagtcaata
     9541 ttttcaaaga tccaaaacag aaaatgagcc aagataaatt ttattttctg atggatggga
     9601 tgattagccg tgggttattc gaaaaagtcg aatgatctgt ggatataaaa agacaactag
     9661 ttttgaaacg gcctattgtt agatgactaa ctttgtaagg acgacgcaaa ttagttgtct
     9721 tttttatttt taaatttaac acatgttgtt atttttaata ttttgctgat ttattgtttc
     9781 taagtaaaga aatttaaatt gaaacgattg ttatttaggg aaaaaggtat tttttgaaaa
     9841 aagaatcccg tataatgatt gttgtgcaaa cgctatacaa aatgaaaaag aaaagaggga
     9901 tgtttatgga aaatgaacag tcggtcgttt taacgaattg gaagagaaat tatttgttct
     9961 ttttatcggg acagtttttg tcaggaatca ctagtatggt cgtccaatat gcgatcatct
    10021 ggtatttgac aagagaaacc ggttcggcga caattttgag ttttgctact ttactaggaa
    10081 tgattcccat ggttttacta agcccatttg taggaccgct agtggatcgc tgggataaaa
    10141 aagctctttt gattgtcacg gatattattg tagcgatttt cgcgcttatt ttagcagtag
    10201 tcggaacgat ttcagaatcc ttcccgattt ggctagtttt tgtgtctttg tttatgcgtt
    10261 cggtcgcaca aacattccaa atgccaacga tccagtcgat tatgccgaca atcgttccat
    10321 cttctcacat aacaaggacg aatggacagt taggaatggt ccaatcagcg aatttcatca
    10381 ttgcgccagc tctaggtgcg gctttgtttt cagtcgttcc tgtcaattac cttattttat
    10441 tagatgtatt gggggcagtt tttggtgtcg gcttattgat ttttgtgaag atcccaaaag
    10501 tttctcctga aatattggaa gttccgctta ctatttttaa agatgcaaaa tttgggctgc
    10561 agcaattgat ggataataaa gggttatggt acataacgat caatggtgca tttgtgatgc
    10621 tgttgttcat gccagcaatc agtctgtatc cattgatgac gctagattac tttggtggtt
    10681 ccgttggaca ggcgggagca gtggaagtag tctacgcagt cggaatgctc ttaggcggtg
    10741 cactgatcag ttttatagga acatggaaag accgaatgaa acctattatt atagcctaca
    10801 tcattatggg cctgacgatc ggtgcaagcg gattggttcc aaacgatagt caagggttct
    10861 tgtactttct tatcttgaat gcaggagcag gttgtgcaac gccatatttc aatacactgc
    10921 tgatggcaat gatccagcag agttatgaat cgaatgtttt aggtcgtgtc ttagggaact
    10981 tcaactcttt gatgaacctg gcaggtccaa ttggattatt atttgcagga ccattagctg
    11041 atcgtttagg ggtagaaaaa atgttcttgt tttccggaat cgggatttta ttatgcggaa
    11101 tcgttttatt tttaacgtca gctgcccgaa attatgataa agaattacag aaaaaactgg
    11161 taaaagaaca ccatgagcaa aaagacgaat agtatggaat ggagttgtga cagaagtctt
    11221 gttacaactc catttctgat cctaatgttc ctgtggcaat aagcctactc aaatgaaaaa
    11281 tatgagtaat tatctttatt tgtttaatac ttgatatagg gcagtttttt cacgacctat
    11341 cgtacttcct ctttgcttca tagatttctt tgatttgttg ctcagataac ggagtgccaa
    11401 atggtagttt ttcctttaag agttctcttt ccggtgcgtc tacgcctaca ttgacatcgt
    11461 tctcgatagg tacgctgtaa tgatggatat gtccatgaag attgcggata gtgtcgttag
    11521 gcccgagcaa catcggataa tgagtcaagt aatattgttg atggtcgaat ttaacgatgg
    11581 cacctacatc ataaaaataa aatttgggac ttccgtcagt aagcctagga tcattttttt
    11641 ctaaataacg gaaaaaagcg cgactgtcat ggttcccttt gatcaagtgg attgttcctt
    11701 gcagttgagt gaccagttcc aaaatttctg catttgcttt ttcatattgc ggatgcatag
    11761 caaggtcgcc tagatgataa acatggtcgg tttcttttat tactgcattc cagctagtga
    11821 tcaactgttg gttcatttca tccacatttg caaagagccg cggagcaaag tcgttttttc
    11881 ctaataagcg ttcatggaaa aagtgcatat cactgataaa gtaattcatg tatcttttgc
    11941 ctcccttttt tcttagagta aacaaaatca cttatctgag caactcataa catctaaaaa
    12001 gaaaaggttg taaatgagac gtgcagcttt aagcgataag aagcaatttt gaaaaatagt
    12061 ttttcacaat ttataaaatt attccttatt gccgaaaggc tggccctttt tatgagtatg
    12121 ccaatttttt atatcgaatg ctcaaaagat gagtgatttt ttggttgact atcatgtttg
    12181 tgtactgaat aggtccagta gaacagtatt tttacgactt gataattaag ggatgaaatt
    12241 ttcagaaata tgacgtataa tgtaaaatac tgaaaagttg cgaccttcaa ctcgtagatg
    12301 tttcagaatc taattgaaat gatggtgata tcatgacaga caacagcaaa acacgcgttg
    12361 tcgtagggat gagcggcggt gtcgactcat ctgtaacagc acttttgtta aaggaacaag
    12421 gatacgacgt gatcggaata ttcatgaaaa attgggatga cacggacgaa aacggtgtat
    12481 gcacggccac ggaagattat aaggatgtag cgaaagtagc tgctcaaatc gggattcctt
    12541 attactctgt gaattttgaa aaagaatatt gggatcgtgt atttgaatat ttcctagccg
    12601 aataccgcgc aggacgtaca cctaacccag acgtgatgtg caacaaagaa atcaaattca
    12661 aagcattctt ggattatgca atggatcttg gcgccgatta tgtagctact ggacattatg
    12721 cgcaagtcac tcgcgatgaa aacggcgtgg tccacatgct tcgtggagta gacaataata
    12781 aagatcaaac ttacttcctt agccaattat cacaagaaca gctttcaaaa acgatgttcc
    12841 ctctaggcgg catggaaaaa tcagaagtac gggcaattgc tgaacgcgca ggccttgcga
    12901 ctgctaagaa aaaagattca acgggaatct gctttattgg tgaaaagaac ttcaaacaat
    12961 ttttgagcaa ctatctgcca gcaaaaaaag gaaatatggt cactcttgat ggtgaagtca
    13021 aaggacaaca tgctggcttg atgtattata cgattggtca acgtcaagga ctagggatcg
    13081 gtggcggagg agattcgcaa gaaccctggt ttgttgtagg aaaagatcta gcgacaaaca
    13141 cattatatgt ggggcaaggg ttccatcatc ctgcattgta tgccacaagc ttggatgcaa
    13201 gtgaaatcca ctttacgacg aatgaaccaa tgccaaaaga attcaagtgt acagcgaaat
    13261 tccgttatcg tcagcaagac gttcctgtga cagttcgctt attggatgat aatcgtgcag
    13321 aagtcgtatt cgacgaacct gtacgtgcaa tcactcctgg acaagcagtt gtattttatg
    13381 atggaatgga atgtctgggt ggtggtttga tcgaccacgc atatcaagaa acaaaagttt
    13441 tacaatacgt ttaagcgata ttaaatgaat caaaacagct atggctaaca aaagaaacac
    13501 cttttctttt ggatagcata gctgtttttt tgcaataaaa ttaattttta ttcattttat
    13561 acttgaaata cttgtgtaaa aagttcataa tatccaataa tatgaataaa gttgcttttt
    13621 gttaagcaag agcagttgca aaatagaagg gaatcttttg caagtgattt gttcaatttt
    13681 ataagtgagg gtgatggaaa tggaaaagaa atcgacgggt attagcaaat ggggactggt
    13741 tgctctggtt gtcagttcgt caatcggaag cggtgtgttt gggatcacaa gtgatctggc
    13801 tagttctgca gctccagggc cagcgatcct ttcatggata atcgttggga ttggtatttt
    13861 agctttggta ttatcgctga atcatttagg agaaaagcgt ccagatctag atggtggtat
    13921 tttcggttac gcggaagctt catttggtaa attaggtggc ttcatcagtg gttggggtta
    13981 ctggttgtct gcgtggctgg gaaatgttgc ttttgcaacg atgctgatga gcgccatggg
    14041 agaatttttc ccgatattca aaggtggaca aaatgttcct tctattttat ttgcaagtat
    14101 ttttatttgg ggtctgactt tattagtaaa caatggagtt gaaggagcga gcgtcatcaa
    14161 tacaatcgtg acgatctgta aattagtgcc attattccta tttttaatat ttgtatttat
    14221 cgcatttaaa gtgaatatat tcactgctga tttttggggt aacgtatctg ataatctagt
    14281 gaatggaaca gggcagcaag gaacgatctg gttgcagatt aaaggttgtt tgatggttat
    14341 gatgtgggtc tttgtaggga tcgaaggcgc aagtgtttta gccaaccgcg cgaagaaacg
    14401 ttcagaagcc cagcaagcat cgatcattgg tctgctttgt ttattgttta tctatatcct
    14461 tgcttcgatc ttgccatacg gcgtcttatc tcaagaagag ttagcaacta tttcacaacc
    14521 tgctatggca aatattttaa aaggaatcgt gggaaattgg ggagcagcgc tgatcaatat
    14581 cggcttgatt atttcgatca ttggttcttg gttgtcttgg acgatgctcc cagcagaaac
    14641 aacgatgttg atggctcgag acggtctgtt gccgaaaggt tggggaaaaa cgaataaaaa
    14701 aaatgcccca acttattcgt tagttgttac tgcaatcttg actaatctat tcttattgac
    14761 tttcttagtt actgattatg cctatcaatt tgcctattct ttatgtacag cggcaatttt
    14821 gatttgttat ctacttgtcg ggatctatca agtccagttt tctttaaaag aaggaatcaa
    14881 aaaacaagta gtgatcggtg ggattgctgt agtttttgaa ttgatgggca tcattttagc
    14941 aggattttct tatgtccttt tatgtagtat tgcctatctt cctggttttt gcttctactg
    15001 gatcgcttgt cgtgaaacca aacatcaaat tacgggaaaa gaaaaagtaa tgattgggct
    15061 gatttgtgtg gccgcactgc tttcaatcgg gttgcttgct gtaggctgga tcaagatata
    15121 agggggaaag aaacatgaaa atcgcaaatt tcggtgtaga agaatggcta aataaatggg
    15181 aaaagaaagc ggtatatgat attgcacaaa gctcgatcga agcattgaca ttagacgaac
    15241 tcgttggctt agatgggact agcgtaagta aatttttcga aaaacgcaaa aacgaaccat
    15301 tggactatgg ctggatcgaa ggatcagatg ttttcaaaca agaagtcgct tctctttatc
    15361 aaaccgtcga tccggaaaac atcttacaaa cgaatggagc gactggtgct aatcttttag
    15421 ctctatacgc gttagtggaa gcaggagacc atgttgtttc gatgcttcct acttatcagc
    15481 aattatatga catccccaaa tctttaggtg ccgctgttga ctttgttcat ttgaaggaag
    15541 aagaagattg gcagtttgat ttagaacagc tagaagcctt agtcaaacct gaaacaaaaa
    15601 tcatctgttt gaatagtgca aacaatccta caggcacatt gctagatcgg aaaactttag
    15661 aaaaaatcgc agaaattgct cgtacagtag atgcatatgt attgatagat gaagtatatg
    15721 cgcctttaac ggataaaggt gaattcttgt cgatcgtaga tgtctatgaa aaaggtatag
    15781 caaccaattc cctatccaaa acctactcca ttccaggtat tcgtattggc tggacagcta
    15841 ctggacctga gttggcagaa gtttttcgaa agtatcgaga ctacacgatg atttgcggcg
    15901 gagtcctgtc agatgatctt gctgtacatg cattgaagaa taaagaaaaa atattggcgc
    15961 gaaacaaaaa gatcattaca gagaaccttg tgattttaaa acaatgggta gccaatgaac
    16021 cgaaagttga acttgtcgct cccaattacg tctctacttc tttcattaaa ttagccatcg
    16081 aagaagatga ccagacattt tgtatcaatt tattagaaga aacaggagta ctattagtac
    16141 caggaagtgc ctttgattta ccgaagcatg cccgactagg ttactgttgt aaaaaagaaa
    16201 ccttggaaaa gggattattc ctcttatctg agttcttgaa aaaacaggcc taaacaaaaa
    16261 cactgcaagg acagtctgtg acaactgttc aagcagtgtt tttcatttac gcttattgtc
    16321 tatgagaagg agagtgccct gaagctatgg agactcctga agctttttct tctgcagttt
    16381 cttgctgatg gatctcttgt gtattgtgtc gtacaagcaa gaagttgacc aaaacaagaa
    16441 gagaagtcga aagaaagacg ccgcgataac caaatgcact ggaaacactg gagccgatca
    16501 taggccctac tacgttacca gttgcttgga aagattggtt ataactgaag atccgtccag
    16561 cagcttgatg aggcgaatat tttgtgatca atgcttggac agcagggagc agacaagcat
    16621 cagagatacc aatcaaaaaa cgtaaggcag ctaattgcca aacattcgtg acgaatgcca
    16681 ttgggatata aaccagaatc gcaaaaccca aaccaatcgc aagtattctt tcactcccaa
    16741 tcttgtcacc taagcgacca aatcttggag cggctatcaa agtggcaatc ccaggtatcg
    16801 aagcaatgat tccgctgatc aacgtaacat tcccatgtcc atgcagcaat tggcgaatgt
    16861 acagactgat gattggacta atggaattgt tggatgcttg gatgatcatc gttgtgacga
    16921 acataccgat gacgacgtgt ggatatttca agtctttgaa aatctgtttg gcagaaacca
    16981 tgttttcttt tttcactggg acaaattttt catggacgaa aaacagactc aacaagaaga
    17041 cgaaaaataa gatgatccct gtaatgaaaa aggtagggcg atagccaaac gctgaagcag
    17101 agatcccgcc aagaagcggt ccaagtaatg ttcctgtaac agaaccagtt gctaaggttc
    17161 caagaacttg accacttttt tcttttggcg tcccagttgc gacaagggca gtggcattac
    17221 tgatataacc agaaaaaatc ccttgaagca gacgcaatgc gactaattga tacacactag
    17281 tcacgcaacc catcaaagaa atgacgattg ccattcctaa tgaagcacgc aacagcatca
    17341 attttcttcc tttttggtcg gctaattttc cccaccaagg agagataagc gtagtcacca
    17401 agaaagtaga tgaaaaagtc aatccgctcc aaaagttcaa ttgtgacgta ctataattac
    17461 ctaacgtatc tatgtaaagt gacataaaag gcatgactaa actaaatccg ataccagcca
    17521 taaatgttcc aaaccataag acaaacaagt tttgttccca agtttctctt ttcctaaata
    17581 atttttcctt tattcttgca gccaattaga ctccttcttt ctgaaaattt tcatctacag
    17641 ctctttatta taaactacaa tatgtagttt atcaatgaaa gtctcttttt tctctttctt
    17701 tttcgtggta aactgaaaga gtgaaggatt tattttatga gaggaaacat ggaaatgaaa
    17761 aaaagaaatc tgtctcaaga gaaaatcatc gactgctttc gagaactagc agaagagatg
    17821 gatgttcagc aaataacctt tcagcattta gccaaacgct tggatatcaa ggctccctcg
    17881 ctgtataacc atttcaaaaa tattcgtgat gtcaaaacag cgctaacagc gaaacttctg
    17941 caggaactta atgaccagct gcgacgggct cttgtgggaa aaagtggtca ggaagcactg
    18001 cagatctatg cagaaactta tcgatccttt gctttttcaa atcaagcagt gtatgagtta
    18061 ctgatcagtg taccgcatac caatgaagct gttttattag atggaatcca tgaaacgaat
    18121 cagatcattt tgcagatatt tgaggctttt caattaacga aagaagaaaa agttcatcga
    18181 agcagggaac ttcgtagtat gatccacggt tatctttcct tacgtttttt aggatacttt
    18241 acaaaagaac caacaatcga tccagaagac agctattttt ggatgatcaa taactttatc
    18301 gctactttgc cttaaacaat cagattcatt tgtaacaatt ttaagatgtt cattaaatga
    18361 cccttttata agagaaaatt cttgcaactt tcttgaaaag tgactaagat gagtctagaa
    18421 aagctgaaag aaggagcacg agtatgtatc aagtaattac catgttcggg gacaatgagc
    18481 cttggtggtt ttttgaagac tgggaagaag atattgaaga agaagaaaca ttcgagtctt
    18541 tcgaagaagc acgacaagcg tacgaaaaac aatggaacga aatccatcaa aattatgaat
    18601 atatcaatgc aaaaagtaac tttctcagcg ccttttggaa tgacggagat gagcgctggt
    18661 gtgaagagtg tgacgatgat ttacagcaat acaagggact tgcgttgttg aaaaatcatc
    18721 agcccatcac agtagaaagc agaaaggaat tttatgaaac aactaattct agcggaaaaa
    18781 ccaagcgttg cgaaagacct aagcaaggtg cttggtgcta atcaaaagca caaaaattat
    18841 tatgaaggac ccaaggtgat cgttacttgg gcccttggtc atttattagg attgaaaatg
    18901 ccggaagatc tgaataagga atggcagagc tggcaaatgg acacgttgcc aatgatccct
    18961 aaaaaactag gaatcaaacc acttcctaaa acaggacatc aattaaaagc aatcaagcag
    19021 ctggctcagc gaaaagatgt ctcagaagca gtcattgcga cagatgccgg aagagaaggg
    19081 gaacttgttg ctcgctggat cttagagtat gtgcatttca ataaaccagt gaagcggtta
    19141 tggatctctt ctcaaacaga taaagcaatc aaaactggat tcaagcaact aagacctgcg
    19201 aaagaatatg atgcattgta tgaatctgct cttgcccggg caaaagcgga ctggctggta
    19261 ggattgaatg tgaccagagc cttaactgtg aagtacgcgg ataatttatc agcaggccgt
    19321 gtgcagacac cgacactagc attagtgaga gaccaagaaa agaaaatcga gcagttccgt
    19381 ccacaaacgt atttcacgat cacgttgcaa gctggaaaag aacaagccaa aatgcttcaa
    19441 aaaaatcaat ttgctttaaa aacacaagaa gaagcacaaa aacttgttca attgatcagt
    19501 acaagcaaag gaactgtcac agaagtccaa gaaaaattaa aaacggaatc tgcgccattg
    19561 ccttatgatt taacagagat ccaaagagaa gccaatcaac gctatggcta ttctgcaaag
    19621 aaaacattaa gtcttgtaca aagtttgtat gagacccata agatcgttac ttatccgcgt
    19681 acagacagca aatatctgac aaatgacatg aaaagtacga tgaaagaacg attgcaagca
    19741 gtcagcgact ttgctcctga ggtcaagcag tatttgaaaa acggagccat cgtccaacag
    19801 acaaaagtat tccaagacag taaagtgacc gatcaccatg cacttttgcc aactgaaaac
    19861 agagcacgtt atgaaaaatt aagtaatgaa gagcgaaaaa tctatcaaat gattgtctct
    19921 cgttttttag gactgtttgc tcaaccccat aaagtcagcc aaacaaaagt tacggttgaa
    19981 tttggtaaag aacgattcat ttttcggcaa aatcgtgtcg tccaagcagg atggaaaggc
    20041 gaaactgaaa ctgagacgga aacagtcaaa tgggaaaaag gaatgagaat caatcccgat
    20101 ttcgccatca aaaaagaact atctgcaccg cctaaaccat tgtcagaagc aagcttgctt
    20161 ggtcaaatgg aaaaatacag tctgggaacc ccagctacgc gtgcagagat tatcgagaaa
    20221 ctaatcaaat ctgaactgat ggaacgtacc cctcaaggac tgcaagtatc tcctaaaggt
    20281 aagcaattat tagtattggt aaatccctct ttagtaacac cagatttgac agaaagctgg
    20341 gaaaaagacc ttgaagctat tgctgcagga aagaaacaag caggaacctt cttgaatgga
    20401 atcgaaaaag aaacaaaacg cttggtgaat gagatcaaat caagcaaaca agaatatcaa
    20461 gatttctcga ttacccaaaa gaaatgccca gaatgtgggg ccaatctgcg cgagaaaaac
    20521 acacgtgacg gcaaaatcta tgtctgcacc aattcagatt gcaactaccg cagaagaaaa
    20581 gatccaaaag tctctaatca tcgatgcccg caatgtcacc ggaaaatgga aatcatcgaa
    20641 ggtaaaaacg gtgcttattt ccgttgtaaa tacgatggaa cgacagaaaa attgcttgat
    20701 aaaaaagagc agaagaagaa aatgaccaaa catgaagaac gacgcttgct gaaaaaatat
    20761 gcacaagcag aagaaccaga agaaagccca ttagcagcag cactaaaagc agcaatgaag
    20821 aagtaataag atatgaaaga ggttgtgata gaagtggtca gcttcaagaa ataagaagga
    20881 atttacgaaa attgcttctc aaatttttgt gaaatttcgg cttatttccg aagaagctac
    20941 ttctggaaca ccatttattc ggaaaaaagg actgtgacaa gacttttgtc acagtccttt
    21001 ttcatttaaa aaaatgttta cttcatttga cgtatattat tggtgaggct tcaattcatt
    21061 tggcttcact ttttctgttt ctcttatgat ataaacagga cgctgtttcg tttctaagaa
    21121 gattttccca atatatttcc cgatgattcc aagacataac aattggattc caccaatgaa
    21181 caggaagatc gagaccatgg aaggccatcc tgatgtagga tcaccaaaca acaatgcccg
    21241 caaaataatg aacaaaattg caataccaga accgacacaa gacaaagcgc cgataaatga
    21301 agcaatcgtt aacggtgcat ccgagaagtt cacgatacca tcaatcgagt agttgaataa
    21361 tttccagaag gaccaagatg tttcgccagc cacacgttcg cggttttcat aagaaatata
    21421 ttctgtttta aatccgaccc agctaaataa tccttttgaa aatcggttgt attctgttaa
    21481 ttctaggacg gcatccacca tttgacgagt catcaagcgg aaatcacgag cgccatcgac
    21541 catttctgta tcgctgattt tattgatcaa ctggtagaat tttttcgcaa agaaactgcg
    21601 tatcgcaggc tcgccttcac gagtcgaacg ccgggtgccg acacaatcga tatcagaagt
    21661 agtgatccgg ttgatcatct caggaagcag ttcgggcgga tcttgcagat caacatccat
    21721 cacggtcact aaatctcctg tagaagcttg caatccagcg tacagagccg cttctttccc
    21781 aaaatttctg gaaaatgaga caaagcggac ataaggataa gctgaggcta gcttcttgat
    21841 tgctgctaac gtaccgtctt ttgatccgtc gtcaataaac aaatattcga actcatgggc
    21901 acctttgaat ttttcaacct catcaaaaaa aagaggaaca gttttttctt cattaaaaca
    21961 tgggacaaca attgatactt tcatcgtttc tatcctttca atcgtatgtt tatatatggt
    22021 ttacattcaa aatttaataa accttaaata acacctttaa aatttgattt tacgtaaaag
    22081 aacaggtatt ctttttgtaa actaacgtat ttagtttacc atgtttcctc ggtttatcaa
    22141 acttttaaga acaattttta ttagttggca agtagttttg gatagagaaa aaggaggaag
    22201 ttccagtcta ttgactagga gcaaacaaaa tgaaaataca cacacctatc ggctttttat
    22261 ggttagatgc agatgaatcc ggattgactg ctgtatcttt tcagccaatt tcagaaagcg
    22321 aatcagcgaa tcagcgaatt ttaacgcgca cacaagaaca gtttgaagaa tattttgcgg
    22381 gaaaaagaaa agaatttgat gttcctttat ctataaagag aggtacgcct tttcagcaaa
    22441 atgtctggaa agctttgtcg accattcctt atggagaaat ccgaacgtat aaagaagtag
    22501 ccgttgctgt tgatagtccg aaagctgtgc gagcgattgg gcaggcaaat cgaaccaatc
    22561 cattgccaat cgttattcct tgtcatcgag tgatcgggca aaatggaaaa ctgacaggat
    22621 atatgggaaa cgcaatagat ggggttgaaa tcaaacgcca attgttgttg ttagaaggat
    22681 atctgcaaaa ttaaaaacag ttgtgattcg gacattgttc catgcgagaa tcacaactgt
    22741 tttttcatgt tcattctaaa ctttgatcag ccaaataaat agtaaagcca tcactgtttg
    22801 gagaagcccg acagctaatc cataagataa gaagcgtgta ccttctttca tgaattttgc
    22861 aaagtctaaa cgcagaccaa ttgccgctaa agcagtgatc tcaaaccagc tgctgataaa
    22921 atgtgccgat tggtcaataa ctacaggtaa agaaaacaag ctgttgacca aacaaaagat
    22981 cacaaaccct aatacatacc aaggaagcgg taattttcgt tttttctgcg tatcagttgg
    23041 cgtttcaaac agagctggtt gtttcttgtg catgaatttt tcaaacagat agacaacaac
    23101 aacgagcatc ataatacgta gaatcttaaa tagcatggcg aattcgacaa cagaattgtc
    23161 aagtaagctt gccccagcga ctacttgacc aacagattgg agtgtaccac ccaaaagtgc
    23221 acttttttca atgagatttc caccaaacaa ctgtaatcca agaaaaggga gagtcaacat
    23281 catcacagta cccagtagat tgactagtgt gatgatctgt cctttttctt cctctttcgc
    23341 atggatggaa ggggcgattg aagcaatagc agaagaacca caaacagcgt ttcctccagc
    23401 catcatcaaa gaagaagtat cgctgaattg cagtttttta ccaatcaagt aagcaccgat
    23461 gatagtgact gacatttgca gtaacacaaa aacaattcct tttacaccaa tttgagcaat
    23521 cgtttgagct gtgacagttg cacctagtaa aaaaacagag aattctaata gtttgctttc
    23581 agcaaatttt gtgccgattg ctagaacagg ctgtttaaaa aaagtatttc ctaataaaat
    23641 acccaataaa atagcaatag tcgctccacc taactgtggc agataaatag caagaagcct
    23701 gctgaaggca gcgatggcaa aagaaagcag taaaccgggt aagatttcgc ggaccgctga
    23761 atcctttaca gattttattg gatgtgacaa tgatttcatt tgatcggttc ctttcgtcaa
    23821 taaatactaa aagcatactc gaacatagta gcactaaacc agtattttga caaaagaaca
    23881 agaggtcgtg acaaaagtct tgtacgacct cttgtttcga ataaacggtg ttccagaagt
    23941 agcttctacg gaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc
    24001 cttcttattt cttgaagctg accacttctt ttacgacctc ttggtaaacg gtgttcaaaa
    24061 gcttgcatct gcggtaataa gcccaaccag tacaaaaaca tgaacagctg tttttgtttg
    24121 gccgttctta ttacttgatg cagaacagct ttttcacaac ctattgaatc gattattaag
    24181 cgattttttt agaaacaaat tttccatgtt tgctatgttc tttaattaag aaacgtacgt
    24241 aatgagcaag aacgtagaag acaactacac ttgcaaacag acattccgca acttttaaga
    24301 aatccaatac gctactatag atacctaaac tgttgaagta atgaacagta gtaaatatag
    24361 ctgttgcaga gataacaagc gggaatgtaa aggctgcata gcttggataa aaacgaagct
    24421 tgaacaaacg tggaagcata gacagcacaa cgaaatacaa tacttgagat aaagtgaaca
    24481 atgctacgac aagataaaaa ttaggttcag caaagttttt aagataacct gttaggcaaa
    24541 gagaaccagg tgctgcaacg atcgtaatca gcggacgtgt cggatgagcc atatttctta
    24601 cgaaaaagac acgtttgatg atgatcggca ataagaacgc atagcagatc aatgaaatcc
    24661 aaaagaaaat ttttccaata agtgggaaga agttcccggc agttacagtg atgaccccaa
    24721 atccaacgta aatgataaac cagcttggat aaattgcttc aattgaaaga tcagctttga
    24781 taacatggtg ataattgaaa taaaagacaa ggatcacttg gaagataaca gcgcacagcc
    24841 aaatgaattt gacaattggt gcaatacttt catatgaaac aaaataagta caaataacca
    24901 tgagtgacat cgtaaaagtt ggtgaaacgg aggcgatgat tggatcatgc aacgtttgtt
    24961 tcgtgtgttc gaataagata aacaatttaa caaaaatgaa tatcataaga atcatagaga
    25021 taccgccagt tagattgcct aaagcaacat ggtgatagtc tttaaacaga tttccaaggg
    25081 aagctaaccc aaggatcaac ccgcagatcg ggatcggaat aattttcaaa aagttcttca
    25141 tatataatat tctccttttt atttaataga ttttagtacc aagatgaacg aatacattgt
    25201 aaaatgaata ggaatgtatg attacgagca agcagacagc tgattgtctt catctaagtt
    25261 actagttgga tcagcaactg ttttgttgta catttctatt atagatggtt ttttgtatat
    25321 ttaaatcaaa tagtttttat agtaattatc ataaaaagtt atggaagtgg tgccatgttt
    25381 aagttattaa cgacttttcg agcagtatat gaaacaagaa atttttccag agcagcagaa
    25441 gtactgtttt tatctcaacc agcagtctcg aatcaaatca aacagttgga gcgggaattg
    25501 aatatcgatc tttttcaacg taacggaaga aaagagatgc ttccaactaa gcaagccgac
    25561 ttattatatg aacggacact ggttttgtta gaagaatggg aagaaactca gcaaaaattg
    25621 ctcaatgaac aaaatgaaga agaagtatgt cggatcgttg cttcccacac ctttgctgtc
    25681 tatcttttgc ctcagttgat gaaacatctg atcaaaaggt ttcctaaagt gcattttgag
    25741 atcgaaatca cgaattctca tgaatcatta gaacaagtag cgaaacatga atccgatttt
    25801 ggttttatcg aaaagccatt agagacttcc ggcgtacaaa gatactcttt gatggaagac
    25861 cagctagtca tcgctgggga cccaaaaagc aaattgtggc ttgtaagaga agcaaactct
    25921 ggtgtttttc attacacgca gcgttatttt gaagagatga atatccaaga taagaagatg
    25981 atcgtgaaaa gtaacgatgt aattgttgcg ttgctgaagg aaggaatcgg tcgctcgatt
    26041 ttgtctaagc gctctgttcc agaaaatgtg ccaatgactc cgttgaattt ccatcgatat
    26101 ttttatttca tccaacgaac caatctggtt tctgatcagc tgctagagat tgccgaagag
    26161 atccttcgat acaatgaaag cacagaaact tctcacagcg aggagaatct cagctggtag
    26221 ataattgcat gtatcaaaaa aagttctatg atacactaaa gataagcagt aggcttttgt
    26281 actgcatttg ctcaagcaac tgcacaaacg gaaagttaat ttttcccaaa tgtctatgga
    26341 aagactttcc attatctaac tggaccgcta taataatcag cagtatggtt tttaagaaaa
    26401 gggatgaaac atggaaaaaa aattaaaagc ttcgaacgat aatcgtattg attatggcgt
    26461 gattttacca gtctttctgt tatgtttgat tggtctgctc tcattatatg tagcacttag
    26521 ccatgatccg aacaatccag cagtcttgaa aggcgtaggg atgcaagttg tctggtattt
    26581 agttggagcg gcagcaatcg ttgtcatcat gcatttgaat tccaaatgga tctggaaact
    26641 gactccttac ctttatggtt tggggttagt ggtaatggga ctcttgctca aattctatga
    26701 tcagaattta gctgcttcaa cagggtctaa aaactggttt cgattcggtg cgtttacttt
    26761 ccagccagca gagttgatga agattgcgta catcttgatg atggcactga tcgtaactaa
    26821 acataatacg caagtaaaag aacggacgat gaaatcggac ttttggctga ttgggaagct
    26881 cttgatcgtg acaataccgg ttttagcatt gatcatggcc caagatgact ttggaacgat
    26941 gcttgtcttt cttgcgatct ttggcgggat tttcctgatg tctggaatct cttggcgcat
    27001 catcgcacct gtagtaatct tggcagtagt cgttggagca ggaacgatct tcttggttac
    27061 gactgaaggc ggaagagact tgctttataa agttggattc aaatcttatc agtttgcacg
    27121 tattgattca tggctggacc ctttccatga tacgtcagga atgagttatc agccggccca
    27181 aggacttttg gcaattggta ccggtggctt gtttggtaaa ggattcaacg taagcaatat
    27241 ctatgtgcct gttcgtgagt ccgatatgat ttttaccgtt atcggtgaaa acttcggctt
    27301 tatcggtggt gcattcgtca ttttcttgta ttttatttta atctatcgta tgattcgggt
    27361 gtgtttcgat acgaataatg aattttacgc ctatatcgca agcggtttga tcatgatgct
    27421 gctgttccat gtctttgaga atattggagc aaatatcgga ttacttcctt taacagggat
    27481 cccattgcct ttcattagtc agggaggatc ctcgatttta gggaacatga tcggtatcgg
    27541 gttgatatta tcgatgagat accaaaatga agcacctgca agatcttcaa ggaggtaaga
    27601 aaattgtaca ccttttactg gtacccgaaa tgttcaacat gcaaaaaagc caaagcgtgg
    27661 ttagaccagc atggcgttag ttatcaaacc gttgatatga tcaaagaagt accgaaggcc
    27721 tcaacgattg agacttggct agaaacgagt caagtaccaa tccgtcgcct gttcaacaca
    27781 agcgggatga aatatcggga acttggactg aaagatcaag taggcgagtt tacggcttca
    27841 aaagcgagcc aagtcttatc gacagatggt atgctgatca agcgaccact gatcttacga
    27901 gaggaccagc ttgttgcaat tggattcaat gaaaaaattt atgaaggaga actgatataa
    27961 aatggcaggc aagcatttga agaaaaagga caacctttgg atcttattta atggaaaaga
    28021 atactgtgtt ggtttgacaa atgaagcgca agaagaactt ggagacatca cgtttgccaa
    28081 tctgccaaaa gcaggacaat cgctaaaaaa agatgaacct ctgatcgaag ttgaagcgga
    28141 aaaagcggtc aatgaatttg ccagcccgct aacgggtaca gtttcttctg ttaatgataa
    28201 aatcggtgaa gatatcaata tcttgaatga ttcagatgaa atgaatgcgt ggatcttaag
    28261 tttcaaagat gttgatccaa aagaatttga cgaattataa aatcagaaaa tcacttgaaa
    28321 aaagttgaca ctgattcttc cagttgctat aatcataagc aatcctatat gaaacgagaa
    28381 agctttgaaa agaagagtac atgtcacgga cgtttgaaga gagccctgtt tgctgaaaat
    28441 gggtaacgaa aatgagatgg aagatggtct tagagcagag taagcgagcg taagcaaact
    28501 tactacggga gtgcccgtta cagcgcgaca gtatacggaa agaagctaag tccaagtact
    28561 ggaagaggct gttattgcga agtaacggta aatcaaggtg gtaacacgga agttcaagct
    28621 ttcgtccttt tgcttaaggc aagagggcga aggctttttt gtttgaaaaa ttataagaga
    28681 aagatgtgaa tggcatgtca ttaatccaac taacgaatgt caaaaagaca ttcaatggga
    28741 aaaacggaaa catccaagcc gtcaatgacg tttcactaaa cgttgaaaaa ggggatattt
    28801 atggaatcgt cggctattct ggcgcaggaa aaagtacatt agtccgtctc ctgaatggac
    28861 ttgagcttcc cacgagcggc gaagtgatcg taaacgatca agatattaca aaattaaaaa
    28921 acaaagaatt acgtacattc cgaaagaaaa tcggtatgat cttccagcat ttcaacctgt
    28981 tatggtcaag aactgtactt gaaaatatcc agctgccgct ggaattagct ggcgtaccaa
    29041 agagcaaacg taaagaacgg gcagaagaac tgttgcgttt agtcgggtta gaaggacgcg
    29101 gacaagcata tccttcacaa ctttccggcg gacagaaaca acgcgtcggt atcgcacgag
    29161 cattggcaaa tgatcctgag atcctgcttt gtgatgaggc tactagcgca ttggacccgc
    29221 aaacaacaga agaagtatta gacttattgt tagcgatcaa taagaaattg aatttgacca
    29281 tcgtcttgat cacacatgaa atgaatgtga tccgcaaaat ctgcaacaaa gtcgctgtga
    29341 tggagttggg gaaagtcgta gaagaaggag acgtattgac tgtcttcaga caaccgaaaa
    29401 aagaagtaac caaacgcttt gtacaacaag acatcgaacc agaagctgat tcgacagaac
    29461 tattagctga actcatcaaa gaaaatccaa gcggccgttt ggcaactttg acctttagcg
    29521 aaagaaatgc caacgagcca gtcatttccc aagcgattcg acgctataac atcgatgtca
    29581 acgttgtgta tggaaagatc aaacaagtga aagaaggttc ctttggttca ttgactgtct
    29641 tgatgactgg tgaaaccaaa gaattagatc aagcagaagt gttcatcaaa gaacaaggag
    29701 taggaattga ggtgatccat cgtggataaa agcttttcag aaacatatct agattttaaa
    29761 caaatcaacc cggaaacgat gaatcaggct atccttgata cactatatat gacattaatc
    29821 tcaatggtct tagtgacgat catcggatta gttttgggat tgatccttta ctctattgga
    29881 cgcaaaaaca aaccgtatgc tcgaatcatt tatggaatcg tatcgatcat cagtaatatt
    29941 ttccggtcaa cgccatttat gatcctgatg gttttgatca ttccatttac taaagcaatc
    30001 gtaggcacta tgctaggtgc gcaagcagcc attccagctc ttgttttatc cgcagcacca
    30061 ttttatgccc gtctagtaga gattgctttt cgtgaagtca gccctggtgt actggaagca
    30121 gcagatgcaa tgggagcaag ttactgggaa atcatctgga aggtcttgat acctgaaagt
    30181 attcccgcat tgatttctgg attaacagtt acaacgatct cgatgatcgg atttactgcg
    30241 atggccggcg cgatcggcgc aggtggactt ggaggtcttg cttggcagga aggctaccag
    30301 cgtggtaatc taacagtgac attcgttgcg actttaatta ttttactaat cgtatttatc
    30361 gttcaaggaa tcggtgattt cttaactaag aaaacagaca aaagataata ggatataggg
    30421 ggatgtagga tatgaaaaag aaaatttttg gatttgcagc agcgcttttg ctgactgtag
    30481 gattagcagc atgcggtaac aacagcgaca gcaaagattc agcaaataaa gcagacgaca
    30541 cgacattgaa agtcggcgct tcaccgacac cgcacgcaga gattttggaa catgtgaaac
    30601 cattattgaa agatgaagga atcgatcttg aaatcgtcaa attcgatgac tatgtattac
    30661 cgaaccaatc tttggaagaa ggcgacattg atgccaatta cttccagcat attccttatt
    30721 tgaacaaaga aatcaaagaa aaaggatatg actttgtcaa tgccggtgct gtccacatcg
    30781 aaccaatggg cttgtactct aaaaaagtca aagatgtttc cgaattaaaa gatggtgcaa
    30841 caatcattac ttcaaattct gaatcagact ggggacgcat catcacaatt ctacaagatg
    30901 ctggcttagt caaagtcaaa gatggagtag atctggaaac agcaacattc gatgatattg
    30961 aagaaaaccc taagaacttg aagttcaacc atacgatcga cccatcactt ttagcttcta
    31021 cgtaccaaaa tgatgaaggt gacctagttg caatcaacgc aaacttcgct tacggcgcag
    31081 ggttgaatcc attagaagat gccgtattgc ttgaaaagga caactctcct tacgtgaata
    31141 ttattgcgac tcgtaaaggt gatgaagaca gtgacaaaat caaaaaactg attgaagtgc
    31201 ttcaccgcaa agaagtccaa gaatggatca cagacaaatg gggcggctct gttaaaccag
    31261 taaaggctga tgcaaaataa ggtatggaac aaaaaagatg agaaataagt ctgtaatggc
    31321 ttatttctca tcttttttat acctattcta atttagaaat caaaattgtt taataataaa
    31381 taaaagatat ttgattttag taaggaaggt cattgcgctt cattctcaat tagtctaaaa
    31441 tgagtgagaa cgaaagagaa tttgggggta taatgcatgt ctgtcttaga aataaaaaat
    31501 ctacatgtgt cgatcgagga aaaagaaatc cttaaaggcg taaacctaac aatgaaaacc
    31561 ggagaaatcc atgccatcat gggacctaac ggcacaggaa aatctacctt atctgccgct
    31621 atcatgggca atccaaacta tgaagtaaca gaaggcgaaa ttttgtttga cgggaaaaat
    31681 gttttagact tggaagtaga tgaacgtgcg cgacttggct tatttttagc tatgcaatat
    31741 ccaagtgaga taccaggtat cacgaatgcg gaattcatgc gtgcagcgat caatgcgaaa
    31801 cgtgatgaaa acgacaaaat ttctgttatg gcatttttga aaaagttaga tgaaaaaatg
    31861 gctttactaa atatgccaga agaaatggct gaacgttatc tgaacgaagg tttttctggc
    31921 ggagaaaaga aacggaatga gatcctgcaa ttgttgatgt tagaacctac atttgcaatt
    31981 ttagacgaaa tcgattcagg tctggatatc gatgctttga aagtcgttgc taaaggagta
    32041 aatgaaatgc gcggagacaa tttcggtgca ttgatcatca cccactatca acgattattg
    32101 aactatatca cacctgatgt cgttcacatc atgatggaag gccgagtagt catgacaggg
    32161 aatgctgacc tagctaaacg tctggaagca gaaggctacg ctggtatcag taaagaatta
    32221 ggaatcgact ataaagaaga agaagcgtaa gggggagcaa gaatgaaaga aaaaaactgg
    32281 ctcgattatc ttgacgcggt aaatgatttt tctttaagta aaggcgaacc tgattggatg
    32341 agaaccttcc gtcaagacgc actagcgaaa gcagatgaac tgccattgcc gcatatcgat
    32401 cgggtgaaat tccatcgctg gtcgttattt gatgtgaaag aaacacaaac catcagtgaa
    32461 acaggcacga ttccggcatt tgatgcaatg aaagacaatc ctgtgcttgt ccaacaaggt
    32521 tcatggacga tttttgaaca actgcctgtg gaattagcag aaaaaggagt catttttacc
    32581 gacttgttca ctgcaatgat cgaatatcca gaactagtcc aagaatatta tatgaaaaaa
    32641 gcagtaaaca tgaacgaaga ccaattgact gcactccatg tagcttttat gaacagcggt
    32701 attttcctgt atgtgcctaa aaatgtcgtg atcgatgaac cactcgagtc cttattcatt
    32761 caagatggcg catccgatga gcacttcttc aagcatgtgt tgatcgtggc agatgaacat
    32821 agtgaatttt cttatctgga acgtttccag acaaccaaag aacaagtagc aaaatcatca
    32881 ggtaatatca tcgtggaagt catcgctaaa gctggttcaa aaatcaaata ttcagccgta
    32941 gatcaattag gagaaaatat tacttcttat atgaatcgcc gtgggcacat tttacgagat
    33001 gcttctgtcg actgggcaat tggtgtcatg aatgacggtc atgtgatcgc tgactttgac
    33061 tcagatttag caggagaagg tgcacacgca gaagtcaaaa tcgtggcaat cagctcgggc
    33121 agacaaatcc aaggaatcga tacacgagta accaataaag cccctcatac gatcgggcat
    33181 attttgcaac acggagtgat ccgagaaaaa ggaacattga cctttaatgg tatcggccac
    33241 atcttgaaag gggcaaaagg tgcagatgcc caacaagaaa gtcgtgtctt gatgctttct
    33301 gataaagcac gtggtgatgc gaatcctatt cttttgatcg atgaaaacga agtaactgct
    33361 ggacatgctg ccagtgtagg acgagttgat ccagaagaaa tgtactatct gatgagtaga
    33421 ggattgcata aagaagaagc agagcgctta gtgattcgag gattcctcgg ttctgtctta
    33481 acagcaatcc ctgtagaaca agttcggaaa gaattagtcg aagtaatcga agggaagttg
    33541 aatggatgat cgatgctaaa aagctacgca atgattttcc tatcttattt caagacgtca
    33601 atgatgaacc tttagtttat ctggataatg cagcgacgac ccaaaagcca aaacaagtac
    33661 tccaggcatt agcgtactat tacgaacacg ataacgccaa tgttcatcgc ggtgtccata
    33721 cgttagcaga acgcgcgacc catgattatg aacaagcaag agaaaaagta cgggcgttca
    33781 tccatgcaaa agaaacagca gaagtgctgt ttactcgtgg tacgacgaca agcttgaatt
    33841 gggtagccag aaccttcggc gaccgctata tcaaagcagg agacgagata gtgctctctt
    33901 acatggaaca tcattctaat gttatcccgt ggcagcagtt ggcaaaacga acaggtgctg
    33961 tcttgaaata tatcgaacta acagaagaag gcttcctgga tatggaggat gcagcgaaaa
    34021 agattacgga caaaaccaaa atcgtctcca ttgctcatgt atccaatgtc ttaggtgtct
    34081 tcaatcctgt tcaagaatta gctgaacttg tccatgaaaa cggcggggtc atcgtagtag
    34141 atggtgctca atctgccccg cacatggcca ttgatgtcca agcactagac tgtgatttct
    34201 ttgctttcag cggacacaaa atgtgcggac caacaggtat tggtgtttta tatggtaaac
    34261 gaaaatggtt agaagaaatg gaacctgttg aatttggtgg cgaaatgatc gattttgtcg
    34321 aattatacga cagtacatgg aaagaactac cttggaaatt cgaagcaggg acaccaaata
    34381 tcgccggcgc tatcgcatta ggccatgcaa tcgattattt gcaaaaactc ggtatggaca
    34441 atatccatcg atatgaagaa gaactagccg cttatgtgtt gccaaaatta caagcaattg
    34501 atggattgac tgtttatggt ccacaagacc cgaattttcg gacaggagtc gttgcattca
    34561 atttggatgg gttacatccc catgatgtcg cgacagcttt agacatggaa ggggtagccg
    34621 tcagagcagg tcatcattgt gcgcaaccgc tattgaagta ccttgaagta tcagcgactg
    34681 ctcgcgcgag cttttatttc tacaacacaa cgcaagacgc cgaccgttta gttgaagcaa
    34741 tcatggcaac aaaggagttt ttccaacatg ggactatcta aattagacaa tttataccgt
    34801 caagtgatct tggatcattc ttcccatccg catcatcgtg gaagcttgac tgactcgaca
    34861 agcaagatcg aattgaacaa ccccacatgt ggagatgtga tcgaattgca agtcgatgta
    34921 aaagacggaa tcatcaaaaa tatcgccttt gacggcagtg gctgctccat cagtacggca
    34981 agtgcctcga tgatgacaga cgccgtcatc ggaaaatcaa tagaagaagc agaacattta
    35041 actgtagact ttctacagct tgtccaagga aaagaagtca aagatcccga ccaattaggc
    35101 gatgctgcta tgcttggtgg tgtagctaaa tttcccgctc ggatcaaatg tgcgacactt
    35161 gcctggaaag cactaggaaa agcaatggaa aacgatggta ccgcagcttt ccatcattac
    35221 cacaaggaag aggagtgaga acatgggcgt accagaatta gaagaatata aattcgggtt
    35281 tcacgatgat gtcgaaccgg ttttcagtac aggagaaggc ttgacagaag aagtgatccg
    35341 tgagatgtca agaatcaaag gcgaaccaga atggatgctt gaattccgtc tgaaatctct
    35401 agaaacattt aacaaaatgc caatgcaaac atggggcccc gacctttcag acatcgattt
    35461 cgatgccatc aaatattacc aaaaaccaag tgacaaaccc gctcgtgact gggaagacgt
    35521 accagaaaaa atcaaagata catttgaaaa gattggtata ccagaagctg aacgagctta
    35581 tctagccggt gcttccgcac aatatgaatc agaagtcgtg taccataata tgaaagatga
    35641 attcgaaaag ttaggaatcg tctttactga cactgactct gctttgaaag aataccctga
    35701 attattcaaa gagtactttt caaaacttgt accgccaacc gacaacaaat tagcagcgct
    35761 gaattcagcc gtatggtcag gtgggacctt tatttacgtg ccaaaaggcg tcaaagtcga
    35821 tgttccatta caaacgtact tccggatcaa tgcggaaaac acggggcaat tcgaacgtac
    35881 gctgatcatt gttgacgaag gagcaagcat ccactatgtc gaaggttgta cggcacctac
    35941 ttattcaagt aacagcctgc atgccgccat cgtagaaatc tttacaagaa aagatgcgta
    36001 ttgccgttac acaacgatcc aaaactggtc agacaatgtg tacaacttag taaccaaacg
    36061 agcaaaagca tacgaaggcg caaccgttga atggatcgac ggaaacctag gtgcaaaaac
    36121 aacgatgaaa tacccaagtg tttacttaga tggaaaaggt gcacgaggca ccatgctctc
    36181 catcgccttt gcaggagcaa accaaatcca agatacgggt gcaaaaatga tccacaatgc
    36241 accaaataca tctagctcta tcgtctctaa atcaatttca aaagacggcg gagaagtaaa
    36301 ctatcgagga caagtaacct ttgccaaaaa cagtgcgggt tcgatctctc atatcgaatg
    36361 tgacacgatc atcatggacg aatggtcgaa atccgatacg atcccattca atgagatcca
    36421 caacagccaa gtctctttag aacacgaagc caaagtctca aaaatctcag aagaacaact
    36481 ctactatctg atgagccgag gcctgaccga atctgaagca actgaaatga tcgtcatggg
    36541 ctttgttgaa ccattcacca aagaacttcc aatggaatat gcagtcgaac tcaatcgatt
    36601 gatcagttat gagatggaag gcagtgtagg ttaaacctgt tataggagct agaacccttg
    36661 atatataagg attctagctt tttttgtttt gcctgtatac tagaaatgac cagcttttga
    36721 ccagctcttt tactgatgag ccatcatatc agcaaagtct tgacctgttt tcttaggtgc
    36781 attatttgta atatgtgcat agatgtctaa tgtcacttta ctattttcat gccctaatcg
    36841 ttgttgaact accttgacag attctcctgc ttctaataac aaactagcat gtgtatgtct
    36901 aaacccatga ggtgtaatac gaggcaagtt atattttttc aaaatatatc ttaaccaatc
    36961 attaacagtg tttggaatgt atagagtgtt tttaactgtt gtaaaaacat gttgctcttt
    37021 agagctagta ttgtatccaa gttttaaata ctcttctttt tgctgtaatt tccagttgtt
    37081 gaggatagaa agagtctcag tatctaaaga gataactcgt ctagaagctc ttgttttcgg
    37141 agattgaatt ataacgttac caaattcatc tacagcaaga gttttattga catgtagttc
    37201 tttattaaac gtatctatgt ctttccactg taaagcgagt acttcactct ttctcatacc
    37261 tgtgtatgcc aataatctga agaaggcgta ttgtttcatg ttaccaaaat ctttaaaagc
    37321 atccagtaaa tcattaagct cctcttttga gtaaaattta cgatttttct catactcttt
    37381 ttttcgtgga agaagtgttt tttccatagg attattgtca atatattgca ttgcaacccc
    37441 gtagcgtaaa atttgtgcag tctgttttct catatagtcg taactttcat atttactgtg
    37501 ccattcatct accacttttt gacaatatgg gacagagata ttcgataatt taagcttacc
    37561 aaatgctgga agtatttgat tatttgcgta tcttactgaa attgcgattg ttgaagcttt
    37621 aacactggta cgctgatgag ctatccattc attatacagg tcttcaaatg tcatatcagg
    37681 tgctttcttt ttaacaccgt ataatatttg atgtctgtat tcgtcataat ctttttgagc
    37741 ttcttgtttg ctactaaaac ctcttctcgt agtatttatt ttttcaccag ttcgtggatc
    37801 ggtggcaatg tgtcctttat acatccacgc cacttgaccg tttttctttt tgtactttga
    37861 tatttttgcc atgaaatcat ctcctcttag tttacaagga gtacttacca ctagatggta
    37921 tcacctccct tcattttttt cttagtgtat tttttcccaa ttgatgaaat cgtttgttct
    37981 agcttgttta tattatcagt aatttctctc acagcttctt cgggataaga catgtctttc
    38041 aattgaatag gtagccattc atctaccatt ctttctagta gttcttctat actgctagtg
    38101 aggaaaagaa tgttattgt
//
LOCUS       DAZKCZ010000012        38029 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000012 DAZKCZ010000000
VERSION     DAZKCZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.012"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(456..1766)
                     /locus_tag="ACG8KN_000554"
     CDS             complement(456..1766)
                     /locus_tag="ACG8KN_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFP9187637.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1778..3196)
                     /locus_tag="ACG8KN_000555"
     CDS             complement(1778..3196)
                     /locus_tag="ACG8KN_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9187638.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3436..5004)
                     /locus_tag="ACG8KN_000556"
     CDS             complement(3436..5004)
                     /locus_tag="ACG8KN_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFP9187639.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5008..5418)
                     /locus_tag="ACG8KN_000557"
     CDS             complement(5008..5418)
                     /locus_tag="ACG8KN_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP9187640.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5415..6338)
                     /locus_tag="ACG8KN_000558"
     CDS             complement(5415..6338)
                     /locus_tag="ACG8KN_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFP9187641.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6335..7213)
                     /locus_tag="ACG8KN_000559"
     CDS             complement(6335..7213)
                     /locus_tag="ACG8KN_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFP9187642.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7200..8537)
                     /locus_tag="ACG8KN_000560"
     CDS             complement(7200..8537)
                     /locus_tag="ACG8KN_000560"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFP9187643.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8540..9643)
                     /locus_tag="ACG8KN_000561"
     CDS             complement(8540..9643)
                     /locus_tag="ACG8KN_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9187644.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9717..11225)
                     /locus_tag="ACG8KN_000562"
     CDS             complement(9717..11225)
                     /locus_tag="ACG8KN_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9187645.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11191..11883)
                     /locus_tag="ACG8KN_000563"
     CDS             complement(11191..11883)
                     /locus_tag="ACG8KN_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP9187646.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11905..12879)
                     /locus_tag="ACG8KN_000564"
     CDS             complement(11905..12879)
                     /locus_tag="ACG8KN_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9187647.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13179..14051
                     /locus_tag="ACG8KN_000565"
     CDS             13179..14051
                     /locus_tag="ACG8KN_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9187648.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14509..16707)
                     /locus_tag="ACG8KN_000566"
     CDS             complement(14509..16707)
                     /locus_tag="ACG8KN_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9187649.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16939..17931
                     /locus_tag="ACG8KN_000567"
     CDS             16939..17931
                     /locus_tag="ACG8KN_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9187650.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17991..19412
                     /locus_tag="ACG8KN_000568"
     CDS             17991..19412
                     /locus_tag="ACG8KN_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9187651.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19405..20904
                     /locus_tag="ACG8KN_000569"
     CDS             19405..20904
                     /locus_tag="ACG8KN_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFP9187652.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20943..22337
                     /gene="uxaC"
                     /locus_tag="ACG8KN_000570"
     CDS             20943..22337
                     /gene="uxaC"
                     /locus_tag="ACG8KN_000570"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9187653.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22496..23179
                     /locus_tag="ACG8KN_000571"
     CDS             22496..23179
                     /locus_tag="ACG8KN_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFP9187654.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRPP
                     LANYLQKGV"
     gene            23181..24017
                     /locus_tag="ACG8KN_000572"
     CDS             23181..24017
                     /locus_tag="ACG8KN_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9187655.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24028..25782
                     /locus_tag="ACG8KN_000573"
     CDS             24028..25782
                     /locus_tag="ACG8KN_000573"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187656.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26058..26399)
                     /locus_tag="ACG8KN_000574"
     CDS             complement(26058..26399)
                     /locus_tag="ACG8KN_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9187657.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26467..28638)
                     /locus_tag="ACG8KN_000575"
     CDS             complement(26467..28638)
                     /locus_tag="ACG8KN_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9187658.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28849..29706
                     /locus_tag="ACG8KN_000576"
     CDS             28849..29706
                     /locus_tag="ACG8KN_000576"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9187659.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29777..30634)
                     /locus_tag="ACG8KN_000577"
     CDS             complement(29777..30634)
                     /locus_tag="ACG8KN_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9187660.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30619..33321)
                     /locus_tag="ACG8KN_000578"
     CDS             complement(30619..33321)
                     /locus_tag="ACG8KN_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9187661.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33334..34623)
                     /locus_tag="ACG8KN_000579"
     CDS             complement(33334..34623)
                     /locus_tag="ACG8KN_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFP9187662.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34773..35819
                     /locus_tag="ACG8KN_000580"
     CDS             34773..35819
                     /locus_tag="ACG8KN_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9187663.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     SYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35821..37974
                     /locus_tag="ACG8KN_000581"
     CDS             35821..37974
                     /locus_tag="ACG8KN_000581"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFP9187664.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11226 a         7827 c         6752 g        12224 t
ORIGIN      
        1 gtttattgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
       61 gttttcaaag gtctacttca tcgagagtgt ttcccgattc atttttggct gccgtagcaa
      121 cttttatatc atatcagaga gacaaacgat tgtcaactct tttttaaaag tttttttcga
      181 tttcgttttt cgaaatcttt cagcaactca tttatcataa cttatcgttt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc gcaagtggtt ctttttaacg
      301 acttagttat cttatcacgt ctaaaaacat gtgtcaactc ttttttggaa ttttcataaa
      361 atgtttcaat gaagcgcgac aacgttgttt aatatatcat taaaaaaaat aaaggtcaag
      421 ctaaaatatt atttaagctt gaccttatta taatttcaac gattgtcaaa agtattgttt
      481 gcagctgtgt tgccagtttc tatagataaa tcacagcttt tgaaacataa gttttgtact
      541 gcttcgattc gcccgaattc ttttgcagtt atttggcaat gagaaaagca gacatcttct
      601 ataggctttt caggaaatgc tactaaatca aaagctcggg aaggaagtga gtaacctgga
      661 gaaaggcatg cctgtacgtt tttgatgtgt atgtctttga ctttcgtcat ttgctttgat
      721 tcaggtattt cttcagccac tgcttgccaa ctttctggca tttgagcaag gtcattgcta
      781 ttttttctat tgtattcatt atgccagtcc attatccaag aaaaaggaaa ttgtacatca
      841 atcatattca gattttctac cctgatattt tcgatcactc cccctctttc ttttgaagat
      901 ttcattcgaa taccacagtc tgactgaaaa aaatcgatat cgtgaatata tacgtcacta
      961 actccagcac taacttcgct accaatagtt acaccgtatc ctgaacggat tttacatcta
     1021 gcaacctcga ttcttgatga ttttcgattg actcttgcac cattgccatc tctgccagac
     1081 ttaatagcaa tacaatcatc accacaggcc aaatcacatt catagacacg cacatttgtt
     1141 gaagaatcaa tatcaatccc atctgtactt ggtccatcat tgttgcggat gattactttt
     1201 tctactaata catcgttaga ataagtgatt tgcaagttcc aaaaacctga tttctgaaaa
     1261 atcaaatcac gaacaacaac atgttctgca tgatacagta aacaagcgcg tggacgttta
     1321 atagcataat ccgcaatcca acgaagtccc ttttgatcgt attcagctct tgtcccgctt
     1381 ttttgatctt ttccccaata cagctcccac caatggtctc cttgaccgtc gattattcct
     1441 tttccttcta taagaatatc tttcgccgag agaacattca aaatcgctgc tggccatttc
     1501 atttctacgc cggctactcg tgtatcgatt tctggaaatg cttcgatatc tgttctcccc
     1561 ttgaggactg caccttcttc aaagataaga tgactcttat cttttaaaaa aagggctccg
     1621 actaaataaa tgccttttgg aataaataca ggtaagtgtt ctttcgcacc ttgatcgatt
     1681 gctttttgca gtattattgt atcatcttgt tgagtgtcgt ttgttttaaa gttcaaagca
     1741 ttgataatcc ctttttttgt ccgcaatacg tttctcccta tctttcattg atttttttca
     1801 attcttcacc aataaataca gtttttgaac gattcagtgg gtaacctact agcaaaatga
     1861 ttgcggatac aaataagcag aatgctggta acaagtttgc catcgtataa atcctttctt
     1921 gtactaaaac tgattggatg gctccacctg ttgtagacga ttggtaacca atgagtgcca
     1981 gcataaatcc gcctaccgct cctgcacatg cttgtcctac ttttctcgca aaagaattta
     2041 ctccatatac tgttccatct tctcttaagc ctgtcttata ttgatgataa tcaatcacgt
     2101 cagtaatgaa tgcccagacc atcaaattga ataaaccaga tcctaaagta gcgataaata
     2161 aaagaatcag atatacccat gcatttgtta tatgaaggaa atacatcagc aagtacatac
     2221 atgaagaagc aagtaacgca aatacgcttg cttccttttt accaaataat tttgtcagct
     2281 ttgtagcaaa tggtgctaaa actactactg ttccgtaagt aaataaaaga gcaacagaca
     2341 ttgcttgttt attatggaag taatcattga ataaataagt catattcgtc ccagctaaaa
     2401 tctgattgat cacaatgaaa atgtcaacaa tgactaaaat aatcagtgct cgattagcta
     2461 acaaaccttt taccaactga gaagcagata ctttttcgtt tttgacaact cttactcttt
     2521 cagtagtcaa tttacaagtt aacatatatg cgacaaatgc tattgcagca catccgattg
     2581 aaatccaaaa gaaatgattc cctgaaacga cttgttgtcc actcttgtta tgggtataca
     2641 ttaaaatcgg aatgaagaat cctgtagttg atccgccaat cgcagaacca atgcttcgga
     2701 acgtggacaa cgaggtgcgg tcattaggat ttgagctaat tgctgctgcc attgctccat
     2761 acgggatatt gattgttgag agaaaaatcc cataaacaat gtatgaaaca aaaatataag
     2821 caatcttcac cgtcattgga agatgttgga cgaatggcaa aaacaaaatg acagtcacta
     2881 agcaaaatgg atacttcatt tttctgatcc aaggaataaa tcggccagtt tctgttaact
     2941 ctgagacatc acataatctt ccaaccatca catcagcaaa cgcatccaaa attctcgaaa
     3001 tcaagaataa cgtacctacg attgcccctg aaacgccaag aacattcgta taataaatca
     3061 tcaagaaact attgactaaa cctaaaataa aacagttacc tagatcaccg aacatgtagc
     3121 ctatcttatc ggaaatccca aattcctttt tttctttttt tattgtattt gctgtaacag
     3181 cattttgtga aagcatttga attcctccta cctcttctag taaacgtttt caaaattata
     3241 actctttttt tcttgttttt atattcacaa tgttggcttt ttattgcatt atcttgacag
     3301 ataaaaaaca aaaaataatc taaaaacatt gatgcataag attgataact ttttttacat
     3361 aacaatatat acaaacaaaa aaattcacta actagtgaaa ataagttagt gaacctcatt
     3421 tattaaagaa ttaaattaca gatgatcttt tccatcatac ttgaaaaact caaaatcagc
     3481 ataacttcct ctttttttat ttagatcatg cactgctatc ccgataaaat ttccagtaaa
     3541 ccctcctgag agtatacaca tgtttttttg cggacctact ttcttccatt cttcagttga
     3601 tacgtctttc aaatgaaact gtgctgtctc tttctttaca gtgactttca aaaaagtctt
     3661 cttttctgtg agtggaatcg tccaatcttc tatttgtgct actccgtttt ctgattctat
     3721 catacgtagg cattttccct tgtcctcatt ccaagtaaca taacaataaa tataattgga
     3781 ttcatttaag tacagtacca gtccagccat ctgattaaaa ttattaggca tgtattcaaa
     3841 tgctgtctcc gcatcaaaag taaaatccgt ttgccgaata gctaacaaat gatgttcgaa
     3901 ggtagattga agagattcac cggaaatcag acgtaaaaaa cctggtctac attttgtatc
     3961 acaccaatca gcatttggca aaatgcgtct ggcattccag ttcttcttta gctctccttc
     4021 aaatgtatcc ataaactgat gatctttttc ttgttgtacc tcttcatttg tctcaatcat
     4081 tatttcttcc atcggagttg tctggctatc tttaagtctt aaccaatcgt cggacgtcca
     4141 atacacttct tgaatagcgg tttctcttcc caaaatagcc gcaccgttta atggtctggt
     4201 acaaagatat gtcatgtacc atcttcctga cggggtttgt actaagctcc catgtcctgt
     4261 acattgcaat gtagaagcag gtttgtcact agctgtcagc ataggaaaac caggatcaag
     4321 ctcgtaagga ccagtaatat tctttgctcg gcaaactgta actgaatgtc ctcttcctgt
     4381 accgccttct gccgtcatca aataataata cccgttacga aaatacaaat gtggtgcctc
     4441 cgttttcgct aattctgtac ctgcaaatat tttatagatg ggaccatcaa gcttcttctt
     4501 ctctgcatca tattcttgta atacgatacc gctggattta ttcggtgtgt ccagacgata
     4561 atcccatagt tcattgacaa gccatttttt tcccgtttca agatcatgga acaaagaagg
     4621 atcaaagccg ttactattta aataaattgg ttcggaccaa ggacctgtga tctcagatgc
     4681 agtgatcaaa taattgtgga catctttaaa cggacggcct gcacttttga catctgtgta
     4741 aacaagataa aacaatccat cagcataact gatctgtggc gcccatatac ttccatttgt
     4801 aggatttcct tgcaaagata cttgagtggt caaaatatcc gttgtatgtg accagttgac
     4861 taaatttttc gactcatata ctcggattcc tggaagccat tcaaaagagg agacaataat
     4921 gtaataccgc tctttcactc ttaatatatt aggatctgga ttgaatcctc ttaaaatagg
     4981 atttttcact ttgatagtct gcatgattca ggctcctttt ctcattctta ctcgatataa
     5041 actgaatgcc ccagctgcga aataaaaaaa tgcaaaagga aacgcaataa actgagtgcc
     5101 aatcaacaat atgaatcctg ttataaaata caaaccagtg gcagttgtta agtattttcc
     5161 tttagcagtc aaaaacaatg ctactgctaa aaaagcaagt gaaataaaca acacaacggc
     5221 aaacagacca gtcatgtcct gtaataatcc aaatgtattt tctaaagaca aagttgaatg
     5281 atctacagca agcgactgat aacttttttt aaactctgac gcagacatat tacttaaagt
     5341 aactgctaac ccgccaaaga aaatcaaaga tagactgcca ccgaccattc ctagcaatag
     5401 ttcccatttt cttttcattt ctgaaccccc tgcttttcta aatgtatcca ttcaggtctt
     5461 ttgatcgatg tttcataatt gttattacgt tcagagaatt tgacagtctg ttcatttttc
     5521 ttgtcatccc aattatccca accagttggg tgaacatgcc cacctatctt acacgcttca
     5581 aataagacat ttgcatacgg acgccacggt cttcctaaat agacgtttct agtatcaggt
     5641 tctgcagtca aggtacagct ttcaaaacga taccctgttt tttgattttt cggcgtagat
     5701 gctgctgtga tgtatccagg atttttactg gctaagctat ggatatgaca ctgatgaaaa
     5761 acggcatctg ccccaccaaa tatgaaatcc accgttcctt caatccagca atattcatag
     5821 gtctgacgac aatactcagg attggcagaa gccggtgtac aaaaaggagt accatctttt
     5881 tgcaaatcag gcaacggacc cgtgcataag gtatcctgat aaccagatag tcgacagttt
     5941 tttaaagata cgcgatctcc tgaattgaat aaagcaacag cctgtcctac cttttctcct
     6001 ggacctgctg tattttcgat gtgaagattt tctaatacga cattcgatcc ttctaagaaa
     6061 aaagtagcac ttcggaaagt ccctaactgg ctaccgtcaa gatactcctg ttttgcaaaa
     6121 cgattaccgg agataactac tgttcctgct cccacaagtt taaaattgga taatctgctt
     6181 tctatatatt catcatatct tccttctttt actatcaaga cctttggatg gctatccggc
     6241 aggcctgcca aataatcgat tccttcttgg atagtttcaa aatccgtcgg ggtatctata
     6301 gcattctttc caattatcag tttgtctttc atcctcataa atgctccctt atgaattgtt
     6361 tccaaatatt tctcatttct tgtgtttcca tatgaccgcc tgttaaagaa aaagaagaaa
     6421 agttttctgg actacccatt gattgatact ttttgtccag cccttttctc agcttcagta
     6481 cgccttcgtc caagcacatc gtatcgtttt ttccaactaa gctaagacga gggcgcggag
     6541 cgattttttc ttggattgct aacgttgtat aagctgtcaa tagatttggc acatagtaat
     6601 aaaacccatg atgatctagg cggcgatgta gcaaaagtat ttcgatgtcc acctgtgccg
     6661 ctagatcaat acaaactttg acacgagaat ccaatgctgt cacccaccag cttaataagc
     6721 ctcccataga aagaccaatc gttgcgagcc gttgagtatc tgtgtcttcc cttgtctcca
     6781 aataatcgat caagctgatc acatcataaa tacgcattcc ccataacgtt tttcctgtta
     6841 ataaaaattg tttgaacaac tcgctttcag aaatacctcc gcgttcttca aatccccaag
     6901 catcaatcga ccaaacagca tatcccattc ctgtcagtac ttctgcaaaa gatggagaag
     6961 ctaagtaagg agcaccatgg atcaattcac ttttcccgac tgaaaaatct ccgccgtgag
     7021 aatgcaggta aaggaccggc ggaaacggtc cttctttttc tggataagca tagagggctg
     7081 gtactgtttc caattcattt aagtacaatt gatagtattc aagcataaaa ccgttttgtt
     7141 tttctcggta taatcgtttt ccactagggg tatgtctgct agatggagca cctaaaaaat
     7201 cattcaactg cataagcaaa gtttttacga acaaccagtt catccatatt ttctgattga
     7261 tttgattgga atttctcaat actgatttct ttagtatttt ctaatagaaa tgctggtccc
     7321 tcgtgatttt caatcgacat ctggttaaag gaaaccttac ccgcaaagcc aatataaaat
     7381 ccttgtctgc tcatttcctc tattcctgtc atcatagcag gaacaccggg aactgcatct
     7441 tgggccatcg agacatcgat attgtcaaat gtgatttctt gtatgaattg ttccgctaat
     7501 ccataaataa aacctgcaga agcatggaca ttccttgccg tgatattcga aaaatggatc
     7561 cttctgaatg ctggtgtccg ttcatctatc ggataagcct ttttttccca tacatacggt
     7621 tctttccctc gcggaccaca gaaataataa agattcagta taaatggaca catcacatta
     7681 tccatgacta tattggatac tcgaatatct tcaacgatcc cgcctcgacc tcgtctagat
     7741 ttcaatcgta ttcctcgatc agtttcttgg aagatacaat ttgaaatggt aatattacga
     7801 atactaccac tcatttcact tcctaatacg acaccgccat gtccatggac catcgtgcaa
     7861 tttgtgatcg tgatattttc gcaagcgatc ctttcgtacg tatcttcagt ccctgccttg
     7921 attgcaatac agtcatctcc cacgtcaata tggcaattac taatacgcac atttttgcat
     7981 gattctggat caattccgtc tgtatttggt gaatccgctg ggttcaagat cgtcaagtta
     8041 tcaaacgttg cattactgca aagaatcggg ttgatcgtcc aacttggtga ctggatcaat
     8101 ttaatatctt tcacagtaat tcgatgacag ttatggaaac tcatcaattt aggacgagga
     8161 taagcgagat tatctggttc atttctaaaa gtatgccacc atttttttcc atttccatct
     8221 aatgtaccga aaccagtgac agagatgttc tctacatttt gagcatagat acaggaagca
     8281 taaacttttc gatggactcc ttcccaacgt gaatgaacaa caggataatc ttttggatcg
     8341 tcagagaatt tcagaaccgc tcctgcgctt aaatgaagtt cgacattgct ttttaaaaat
     8401 aaagctcctg ttagaaattc acctgctgga acaactacgg tcccgcctcc gtcacttgct
     8461 gcagcatcaa tggcttgttg tattgcttca gtattcaatt catcaattga tgcgccaaat
     8521 gtcaaaatat catacatatt tataatgcct ccacttcaat caatgcttgc aagaatgcac
     8581 caacgccttt ttgatcatta caaataattg gttcactgat gtagtaagca tatgaaccat
     8641 ctcgctgatc tgctccgcct aaccctgcta cttgacagtt tttattcaag ttcagccagc
     8701 cttcttttgt ttccagaaca aattcatcaa gtaatccttg atgtgatttc ttcacttgtg
     8761 gaagccattc ttctttgttc agcagtccca aacgtattcc tttagccatt gcgtaaacaa
     8821 acatactact gccggaagct tccagataat ttcctttccg ttttgtttct gttgttactt
     8881 gataccatgt cccgctgttt tcttcttgat atggaagtaa tgcagccagt gtcttttcta
     8941 gaatttcttt cagcaaagaa caatctgttt cttcatgaag taattcatag gtatctgctg
     9001 cagccatcaa ataccagcca atcgatcggc tccaaaaatg atgggataga cctgtttttg
     9061 gatcagccca tggttggacc ttcttttcat cccatgcatg aatcagcaaa tttgtttttg
     9121 gatcgaatag atgtttataa ctgatttcaa actgtctcaa aacatcttgg taatcttttt
     9181 cttcactaaa attctggata aattcagcat aaaaaggaga acccatatat aagccatcca
     9241 accaaatttg ataaggataa atttctttat gccaaaaagc tccttcggat gtgcgtggat
     9301 ggtttgccaa ctgctgggaa aggagttctg ctgctttttt atatttttct tcgcctgttt
     9361 ctttgtacaa taaaaagaaa agctttcctg tatttacatg gtcgatattg tattcttcaa
     9421 cgctgtagcc acggatcgtt ccatcttctt gcacgaaata gtcgatattg tcttttatga
     9481 aatcaaaata aattttttct tgggttcgtt cccaaagtaa ttggaaccct ttaagtacga
     9541 cgccatagtc ataagaccat tttcctttat accacttttc ttcatataag cgaggtgttc
     9601 tttccatgat actttttgct aatttctttg accagttttc cattatttgc tctcctttaa
     9661 aaattaaaat aggatttggg acaaatacca agaagtattt gtcccaatcg tctatattac
     9721 tgtgccgctg ctttgtatgc ttcctcgtac tcttgtgtga ttcgatcgcc tccttgtgct
     9781 ttccagtctt ctacagcttt tttgaatcca tcttcgtcaa gctgtcccat gatgtattgc
     9841 accgtagcat caatgacgat tttttcaatt tctgatccta cttcattgtt cgtcggtgaa
     9901 tccaatggca cagaaggatc cattacagca aattcttcat tttctttgat caattgatta
     9961 gagaattctt tttcagggtc tgtatctttg aaagagaacg tcacttcact aggacgagag
    10021 cttgataacg gttgcacctc ttgttgccaa agatccatat cgataaattc gatagaacca
    10081 tcatcattca attcataatg cgttccttcg ataccgccag tcatcaattt atacacatct
    10141 tcttctaaaa gatcatcaac aaattgaagt aatttcttca attgtgcttc gtcttttact
    10201 tgagatttgg gaaatgctag caagccgcca ataccatttc cttctgacca aacatgataa
    10261 ccgccgcctt caccatctga gatcttgttt acaggcacta attccatatc atcttgaata
    10321 ccttgaccca tcgtcttcag attcttgata tccaccattc cagtgtagat tccagttctt
    10381 ccttgtgcaa attgttcttg ctgatcagtc ttttgggtaa ctgcaaagtc ttgtgccaaa
    10441 tagccattct caaatagatc tcttgaaaaa ttcaaagctt tcatatactc ttctgtatca
    10501 aattctggag tgaatttgcc attatcgtct acagcccagc catttggtgc tccaaaataa
    10561 gaagtgaaaa gtttaaagct ggtataacga atatcactag gcgcacgatc gccaaaacca
    10621 actgtgtcgt ccacaccatt tccatcagga tcgttttctg taaattgttt agctacttca
    10681 taaagctcat ccatggtagt tggaacatct agtcctagat tatccaacca gtcttttcga
    10741 atgactaagc cagcacgagc gtagtctttt tgtatcggta cgccgtacaa ggcaccgtcg
    10801 atactcgcag cttcgattct atcttcagaa atttgactta aatttttata gtcttttaaa
    10861 tattctgata catcccagaa tacgcctgat tttaacgcat tgcggacaga agagtttttc
    10921 aacatcgcaa tttgaagtgt aacgatatct cctagttcat cagaagccaa tgcagttgtt
    10981 aatctttctt cttttgaagc atcaggaatc cagttgaatg tcagttccgt atcggttttt
    11041 tcttcgatca agttcacaat cgtgtctgta ggcggtgaag catgatgcaa aatatttaac
    11101 caagtgattt tagcttttcc atcttctgtc tctgaagaac tgttacttga actgctgccg
    11161 catgcggcaa gtgctagaat tcctgcaaaa ctaagaccta attttacgag cgttgctttt
    11221 ttcataaatt ttctccttca ctgttttaat aatatttttg ttttcttctg ttttttctgt
    11281 atatgagttc ttccattcca aatggcaaaa tatagaattt gctgtccacg tgactgcgga
    11341 aaagaagaga ctgccaccga taaaggttaa aacacctggc attgtttgga tgatcatcag
    11401 ataaatccct accatactta atatcaacac taatgagtaa tgaagatgtg agaatccgaa
    11461 taaaaatgcc attttcattt tgaaaaaata tcctttccaa tcataatgag ccatgattgg
    11521 gaaaatatag agtgctgaga gcacatatat gaacgcagca atatacaaag cgatcctaag
    11581 ataccaattg tgtatgaaag aaagatccac aagtaaaaca atcccgatgg cgccatatat
    11641 ccagctcatc agcaaggttt cacgatagtt ttctttaaaa tatgtgaagt acgttttcgt
    11701 caaaggaaac gtttcttttg agcgcagcca ttttctaatc actgcaacca tcgcatacga
    11761 tgcaggaccg aaagtaaaaa ctcccaaacc tgcgatgact aaaccgcccc acagaaaatt
    11821 caaataaacc aactgtaaaa tacgatttag ccttccgttt attttatcta atgactgcag
    11881 catatctttc cctctctttc tatcttagta aacgccgtct tctcctagct ttttggcaat
    11941 cttattagct aaaattacca taaacaatcc tacgacccct ttaaacaatc ccattgctgt
    12001 actgaaactt aactgtccat tcttcaatcc agctgtataa atataggtgt cgaaaatttc
    12061 tgcaacgctt cgattcaatg aattcaataa caagtacata tgctcgaacc ctaagtctaa
    12121 tgtctgaccg atttttagaa tcagcaaggt aatgattact ggacgaattg ccggcaatgt
    12181 aacatgccat gttttgcgta agcgtcctgc tccatccatt tctgcagctt catacaactg
    12241 tgtatcgact gcagttatcg ctgaaaggta gatgatcgta gaccagccca actctttcca
    12301 gatcacttgt ccgatatata ctgtgcgtgt ccattcaggg gatgttagaa aactgatttt
    12361 ttcaaatccc attgttgcaa ggaattcatt caatacccct ccgtcaacat tcaaaaatac
    12421 ataagtaatc gaaacaatga ttacccaaga catgaagtga gggatgtaaa tgatcgtttg
    12481 aatcccgttt ttcaatcgtt tgttcgttac ttcatttaat agtaaagaca aaatgattgg
    12541 catcggaaaa aacacgaaga tatttaatcc gaacaagatc aacgtatttc ttaataatgt
    12601 aaagaatgtc ggttctgtaa acaaacgaat gaaatgtttg aaaccgaccc aaggacttcc
    12661 cattattcct aaaaatggtt gataatcttg aaaagcaata acgagtcccc ccataggaag
    12721 atacctaaaa atcaagaaat aaaggactcc cggcagaatc atcacatata aaaatttatt
    12781 cgttttgatt cctgcaatta ttttttttcg tcttctgagc ttttcttctt ttttcgatag
    12841 cgtcagttga aatggttctt gtttctttac actttccata agcccctcct tagttttgtg
    12901 aatgtttgct gcttatgact tcatgataga taactatctg aattacgtat ataatcattt
    12961 ttaccgtaag taaaaacgct tacatagcaa tgatttttgt gctatacaaa acgataaagt
    13021 gtattttttc acaaataaac tacgcatagc aaaccttcta tttttttcta tctgacactt
    13081 agcataggaa atgattatat acgtaatctt tcaccgatga taaattgaac atatagaaac
    13141 agaaacattt aatttatttt gaggaggagc ctatctatat gaaaaagaaa gtctctatag
    13201 gagaaagaag ttttacactt ttcaacaata cgtttctaat tttgctagct ttgatctgta
    13261 tcgttccttt tttaaatatc atcgctacat cttttgcttc tacacaagaa gtcgtcgcga
    13321 aaaagtttat cctttttcct actacctttt ctttagacgc gtatcgctac attctttcta
    13381 cgccgaccat ttttagagca cttgctgttt cgattggtgt tacaggtgtc ggaacgatcg
    13441 tcagtatgtg tgcaacttca ctgatggcct acggtctttc cagaaagtat ttgtttggtc
    13501 gaggtttcgt caactttctt gttgtatttt ctatgttgtt cagcgggggg atgattccaa
    13561 cgttcttagt tgttcgttct ctagggctgg tcaattctta ctgggccatg atcctgcctg
    13621 tggctgttaa tgcaatgaat atgatcatta tgagaaactt tttccaagcc ttgccggata
    13681 gtttggaaga atcagcaaaa atggatggtt gcacagattt tggtgtattt ttcaaaatta
    13741 tgctgccatt agcgttaccg tctattgcta ctatctcgct tttttatgct gttacttact
    13801 ggaacactta tatgactgct attttgtata tcaacgattc atctaaatgg ccgatacaga
    13861 ttcttttacg ccaaatcgtc attgtatcaa gtgggatgca agcagaaagt agtgccgttg
    13921 atgtgattcc accagcacaa acagttaaaa tggctgttat cgttattgca acagtgccta
    13981 tgctgattgt ttatcctttt gttcagaaat attttgtcaa aggagcactc gtaggttctg
    14041 tcaaagggtg attatgtact gaacgcaaaa tggtagttgt gtcagaagtg gtcagcttca
    14101 agaaataaga aggcatttac gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt
    14161 tttcgaagaa tctactcctg gaacaccgtg tatcagaggt tgtgaaagag acgttcagcc
    14221 ttgagcgatg ataaaaactc tcacaaacat tgcgacgagt aacccacagg agcaataaga
    14281 agcgattttg aaaaatagct tcccatattt cacaaaatta tgccttattg tcgatagcct
    14341 gtctcttgaa cgccatttat tcgtaaaaaa gaactgtgac aagacttttg tcacagttct
    14401 ttttgcgtgc ttagccttga aatttaacta tccataaata caaatactag ggttattcta
    14461 acttctttta ttgcttgaat cagtccacaa gaaaaacagc ctcttgattc acaaatattt
    14521 ttttcgatat tctcccggcg taatcgattc cttcttttta aagaaacgaa taaaattttg
    14581 cggatttcga tattgcaatc tttctgcaat ctcttttacg gagaggttag tctctttcaa
    14641 ccattttttt gctacctcta aacgatgatt ttgaacaaaa tcaccaaatt tttctcctgt
    14701 ttccttttta aatacattac ttaaatagtt agggttataa tgcaacctat cagcgatcac
    14761 atctaaagac agatcacgat catattctgt gtagacgata tgcataatat tctccgacaa
    14821 atttttaaat tcatgatcag tgattgcttg agagcattct gtgattggca gaactaattg
    14881 ttctaaaaca agtttttcta gttctttggg atgataattg ttcagtgctt ttacataaat
    14941 ttgtttcatg ttatcaaagt tttttgaatc tactcccaat aactgtccta attgaacgag
    15001 ttcattgatc aagcggaccg aaactacttc ccgactcaaa ggatttttat tttgtgcaaa
    15061 caatgcatca atcaattcat gtactaaatt cttcacttcc tgatttccag aacgaatggc
    15121 ttcaaacaat cgattttgtt cttctatcgg atatctaata agtgtttttt catgagcagc
    15181 aggaacgatt tcctgataaa aaataattga acttggacca gtatttacac gataatacag
    15241 ggcttctttt gcccgatcaa ccgattgctt gctttcattc agcgtttcga accggttact
    15301 gatgcctata ctgatagtta aattcaagta ttcttttaca cgtttttgaa tgagtcggca
    15361 atactccatg acttttttgc ttgcttctgc atcatcttta tccattcgat aaatagtggc
    15421 ttgcatttct tcattcaaaa cgatcggcag cattcgattt tcctttggca caatttccgt
    15481 gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc ccaccgtgac tatcttctaa
    15541 cagatcgatt tgaaccaaag ctgtataata gcagtcattt tgagaactat aaccaaattg
    15601 ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt ctaaataaac taagtacaaa
    15661 tagcgtttcc agctgtggct tttgggtaac taagtgtgca cttaacgctt ctttttctcc
    15721 tatgatccga ctgactgatt gagcgaccaa atctagttct ttcccactga atccttttga
    15781 tttaagattc agacgttcct gtatttgaga aatcggacgg gcaaatctgt ccgaaaataa
    15841 gtaggaaaga agaccaatga gtcctaccag tgttaaagac actgcaacta gcccgattct
    15901 cagattgcgg atcattgcgc caattgcact agagtcaact tctaatctgt aaatccaatt
    15961 attataatct gatttcagta ctatatacgt tttatctttt tctcttatca ctttcagctg
    16021 attgtaatct agatccacgt ccgttaatac agttggttgc tctttatttt ttactgtctg
    16081 agtagtaaaa aggttgccaa cttcgttttc aatagaaagt atgtcttctc cactttcacc
    16141 aacaacttgt tctattgtat gtttctctat cgtagcaatt cccaatgcat atttttcttt
    16201 ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg tttttctttt gccaaaataa
    16261 attatttttg ttatttatgt acttctgttg atacttttca atttcctctt cggtcagttg
    16321 actcaacgat ccattgatta ctccccattt accagccaaa ctgactaaat cataattatt
    16381 atcatccata acaatcgttt ctataaaatt tagttctttc ctgatttcag aataagtgga
    16441 gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag aaatttttgg tcgaaccata
    16501 cgtagaaaac gaatattcaa tcgttctaat tttccattca acgttagttt taacttgcct
    16561 gagcaaattc tttttgtctg ttaaataact cgattcgatc gattcatagg tattctggta
    16621 aatgaataaa ctaaatccta tgacaagtac ggctcctaat aaaaacatcg gtaaaaatat
    16681 tttataaaat actgtacttt tcttcatttt cttcctctcc tccttttaaa ataaaaggct
    16741 ttcatttttc tgtaaaattg atactcatct aattaaatga gcattccccg ctttcaagaa
    16801 tagcataatt aaaaataaaa atccaagtaa tttttaacgt gtgcaatctt ttcatatcaa
    16861 agatctattg acttatcgta gttatcgtaa tatgatgaat gcgttaacaa aaagtgaaaa
    16921 agtaaggaaa atgatggaat ggtaacgatc aaagatgtcg ctaaagcagc tggcgtttct
    16981 catacgacag tttctcgtgc tttgaataac agcagtttaa taaaaaatga aacaaaacaa
    17041 aaaatacgac aactagctaa ggaaatgaat tattcgccaa actatagtgc aaaaggttta
    17101 gtgaatcaaa aaaaatatct aatcggacta tttttctcaa gtttgcataa aggaacttca
    17161 tcaaatttcc tagcagattc cattgcaggc atccattcag tattagacac aaattacagt
    17221 ttatctgttg aaagtatcga tgttgttgat ttaacagaaa tcaacctgca acgctacgat
    17281 ggactgatcg ttatgagtca aagtgatgat gatcagcctt tcatcgacta tctcaaagaa
    17341 aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg atccagcggt tatcaacgtc
    17401 actgccgatg atgcggccgg tgttatgcag gcagtagatt acggtattgg atgcggacat
    17461 acgaagatcg cttatattgg cggaaagaaa aattttcgtt cgacaaaaga acgggaaaaa
    17521 ggggtattga aaagtctaaa aaaagcgagc atccctgtca atccagccta ttttttaaca
    17581 ggagattaca gcttagaaag cggcctccaa aacatggaat acctattatc actttctgac
    17641 ctgcctactc tggttttttg cggcaacgac gacatagcga ttggtgcatt gagagctgta
    17701 catttagctg gcctccgtgt tccccagaac atttctctga tcgggttcga tgattctcca
    17761 gtcgtctcct atctcaatcc gccgcttacc accgtacgta agcctttgaa agaaatgtgt
    17821 caagaaggaa caaagctttt actagatttg atcgaaggca ggacgatcga acaatctaaa
    17881 attgagctct ccaccttgtt gatccttcgt gagtcgatca attacttgta aactacaagt
    17941 aattttatat aaaaatgtta acgtgtgcat aacacagaaa ggaagtttat atgaaaaaac
    18001 tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa agttcttcag tttggtgaag
    18061 gcaattttat gcgtggattt attgattggc agattcagca gctgaataac caacaattgt
    18121 ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg tcttggaaat atgatgaacc
    18181 agcaagataa tttatatacg gtcattttac aaggactcag caatggcgaa atcgttgata
    18241 cttccgagat tatcaccgta gtagaacaaa tgatcaatcc ttatgatgaa tgggatgctt
    18301 atctatctct agcagaaaat gacgacttgg cctatatcgt ctccaataca acagaggcag
    18361 gaatccaatt cgtggagact gataatttaa cagatactcc cccacagagc ctcccgggca
    18421 aactgacagc ctttttatac cgacgtttca aattaaacaa agctggcttt accatcattc
    18481 cttgcgaact gattgacaga aacggcgaac gtctaaaaga aatcatttta cgctacgcag
    18541 aaaaatggga tttagaacct gcttttgttg agtggttaga aaaagaaaat gttttctgtt
    18601 gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga agatgcaggt gttttcaatc
    18661 aacggcataa ctatgaagat caattgatgg tcaaagcaga accattcatg ctttgggtga
    18721 tcgaagggcc taaagaattg gagaatcaac taccattcaa acaggctggc ttgaatgtca
    18781 tcatcacaga tgatatgact ccttatcgtg aacgtaaggt gcatttgctt aatggaccgc
    18841 atacagcgat ggtccctctt gctttgttgg cggaattgga aactgtagaa gaagtaatga
    18901 aagatccttt attctccgca tatgttgatc agttattcaa tctagaattg attcctatgc
    18961 tttctttacc taaagatgaa ttagccattt atgctgacca aatcaaagaa cgtttcttga
    19021 atccttttgc ccatcacaaa ttagaagcga tttcattaaa tagtgtttca aagttcagca
    19081 cccgtctgct tccagtgttc aagaagtata tcgaagaaca aaatcaggta ccaccgttaa
    19141 tcactgtttc actagctgcg ctacttctta tgtatcgagg cgatcaagta aagccacatg
    19201 atgatgaaaa gacgataagc gaatttactg atgcttggtc tgataatgga acagctattc
    19261 ctcgattatt gcaaaacgct gcgctttggg gagaagattt gtctcaaata ccgaatgtga
    19321 cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa aggtgtccgg tcagtattac
    19381 aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc aaccaacaat gaaattaaat
    19441 cgacgagaca gtgtggtcgt cgcattaacc cctattccaa aacaaacaca actgacgatt
    19501 gatcagcaaa cgatcaccac tcttgaagat atcccgcaag gtcataagat tgcattgggt
    19561 gatttgaaag ctggagacaa cgtgatcaaa tatggctatc caatcggtca tgtgacgaca
    19621 gacgtaactg ccggtcaatg gctgcacact cataatgtaa aaaccaatct ttctggtgaa
    19681 ttggattatc gatatgaaaa agatgtccat ccaagtcatt acctttttga gaaccgaaca
    19741 tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc gtaatgattt gtttttagta
    19801 cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg tcaaacagtt caaggaaaaa
    19861 catccagatc tgggacagtt tgaccatatt acgattttaa agcatcctta tggctgttcg
    19921 cagcttggaa aagatcatca aaatactcga gaaattttag cggacgcagt aaatcatccc
    19981 aatgccggag gagttttagt atttggatta ggttgtgaga acaatacagt tcctgaattc
    20041 aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat ttctagtggc tcaagatgtt
    20101 tacgatgaga tcgaacaagg tgtggctcta ttagaagagc ttttaacagc ggcggaaaat
    20161 gaccagcgca tatcagtccc gctctctaag ttgaatgtcg gtttgaaatg cggcggttct
    20221 gatggtctat cgggaataac tgctaatcct ttacttggtg cattttctga ctacttgatt
    20281 gctcaaggag gcagcacgat cttaacagaa gtgccagaaa tgtttggcgc agaacaagta
    20341 ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa tcgttgactt gatcaatgac
    20401 ttcaaacagt atttcttgtc ttacggggaa ccagtttacg aaaacccatc tcctggtaat
    20461 aaagcaggtg ggatcactac attagaagat aagtctttag gctgcacgca aaaatcagga
    20521 agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa tcagaaaacc agggcttagt
    20581 ttattgcaag ctcccgggaa tgatcttgta gctgcctctg ctcttgcttc atccgattgc
    20641 cagttggtct tgtttaccac aggaagaggg actccgtttg gctcatatgt cccaacggtc
    20701 aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc attggatgga ttttaatgca
    20761 ggcgtcttat tagaacaacc aatggaagta atcttagaat cgtttgttca aaaaatcatc
    20821 gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg acgttcgaga aattgccatt
    20881 tttaaaaacg gagtaacact ttgattttca taaaacagta taaaaagaaa gaaggaagat
    20941 ttatgttttt atctgatgat tttttattaa aagacgattg ggcgaaaaag ctttaccatt
    21001 cctacgcaaa aaacatgccc attattgatt atcactgtca cttagatcca aaagaaattt
    21061 acgaaaacaa caatttcagc aatctgacag aagcttggtt aggaggcgat cattacaaat
    21121 ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat cacaggaaat gcttctgatt
    21181 ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat tatcggaaat ccgctttaca
    21241 gttggtcaca tcttgaattg aagcgtttct tcggtattga cttgttgata aatgaagaaa
    21301 atgcagagaa aatttggaca caagcaaatg aaattttggc agctcctgat tttcgccgtc
    21361 gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac aacagatgat cccgcagacg
    21421 acttgcacta tcatcagttg cttgctcatg aagaaccaga tttgaaggtt ctaccttcat
    21481 tccgtccaga taaagcactc aatattgaaa aagaaggatt taatgcatgg gtcacactac
    21541 tggaagaagc agctgaccaa acgatcacca cctatcaaga actgatagaa gtcttagggc
    21601 agcggataga atacttccat caacatggat gtcgtgtttc cgaccacgct ttagacatct
    21661 tacgctataa agaagcggat gaaacagtat tagaagagat ttttcaaaaa gcacgagcta
    21721 acaaactatt atcttcagat gaaatcgatg cttatcgtac agaaacactg atacatttga
    21781 cccacttcta tcattctcat aattggacga tgcagctgca catccatgct tatcgtaact
    21841 gtaatacaca aatgttggaa aaattagggc ccgataccgg ctatgatgga atgaatgatc
    21901 tttctttgac tattcctctg caaaaattat tgaaccgtgc agaagaaacg gatcagttgc
    21961 caaagacgat cctttattca ttgaatccga atgactatcc tgcgatttta gctttgatgg
    22021 gctgtttcca aaaagaacag aatggaaaac tgcagttagg ttctggatgg tggtacaacg
    22081 atactcgagc tggtatgcgc gatcaattga cacaatttgc tgatggtagt gctttgggga
    22141 attttgtcgg catgctgaca gattcaagaa gtttcctgtc ctacactaga catgaatatt
    22201 tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat gcgaggagaa gcacctgaag
    22261 atgaacaact tttaggaagt ctagttcaag cgattagtta tacaaatgct aaaaattact
    22321 ttggcttttt tgcctaattg attaatagta acagtaacga atcaaaaaca ggatagtgaa
    22381 ataacggaat ccgtttatcc acgttttaga gaagaagacg gaccaaattt gctccgtctt
    22441 cttctcttgc tgttctgctt cattgcctat aaacctttat gaaagaagag ataacatgcc
    22501 tactatccat attgccggtg attctactgc ggcagaaaaa gatgaaaaca agcgtcccga
    22561 gacaggttgg ggagaaaaaa tctctgctta tttttcaccc gagatccaga tcaataacca
    22621 agcaaagaat ggacgcagca ccaaatcatt tatccaggaa ggaaggttag cccgtctagc
    22681 ttcacagttt cagccgaatg atttcttatt tgtacaattt ggacataacg accagaaaat
    22741 ccaagaagaa agaggtacgc ttcccttcgg tagctattta aaaaatctgc agatattttt
    22801 tgaatgcgca aaaaaagcga acgtccaaat ggtcttactg acatctgtga cgcgacgaga
    22861 ttacttagaa aacggaacat tgaatccaga tatacttgga gactatccaa aagcgatgcg
    22921 tgcatttgct gagaaacacc atattcctct tttagatgtg ttctcaagat cacaagaatt
    22981 gttccagacc ttctcaaaag aagagacaaa aaaattctac ctgcatctta tacctgatac
    23041 ttgtaaaaat tacccagaag gactaaaaga caatacccac tttagtccag aaggcgctga
    23101 taaagttgcc cgaataattg ttgaattgat aaaagaatcc cgtccgcctc ttgctaatta
    23161 tttacaaaaa ggagtttgag atgtttccat taaatttagg catccgtgcc cacgatttag
    23221 atgttcgaaa tcgtgaagaa atcgttcaaa aagtatcaca gcttcacttg tcccatatcc
    23281 aatttgcacc acaaaaagca tttcctgaac aacttaaatt aactgacatg acacttggga
    23341 gtgctgccta ttttggtgaa tacttccaaa aaaacaatat cgaactttcc attttaggat
    23401 gctatatcaa cctatccagc aaaaatcaag aagttcgtgc acaagcattg gcaactttca
    23461 aacagcaaat tgctctgtgc tctgcctatc aagccaagat gattgctacg gaaaccggta
    23521 gtgtctctgt cggttacaca aaagaaaatt ttactgaaga agcttatcag atcgccagaa
    23581 attctatcat tgaattagtc gctcatgcag aaaattttgg cgtaactgtt gctgttgaag
    23641 cagggatcaa tcatccaatc tataactatc agttagctaa acgtttaatc gatgaagtag
    23701 cttcaccaaa tttaaaaatc attttagatt gtgcgaattt gatgcataaa gaaaatcatg
    23761 ctgatcagga aaaagtgatc cgaggtgcct tggacaattt acatggttat attacagcgc
    23821 ttcatctcaa agactatatc atggaaaatg gaaagatccg catggtacct gttggcaagg
    23881 gctgtttaga tttccgcccg gtactgcatt atatcaaata tgagcgtcct ctaatgtacg
    23941 ccactcttga agctactcct gagatttatg ttcctgaagc aatagaacac ttgcagttta
    24001 tttaccaaga ttgttaagga gaagattatg gaggaacgaa tacagaaaag ttatcaaaaa
    24061 tatcatgcaa atgcttttca gacattgctg catctttatc agggaaatta ttggcgtttt
    24121 gccatgtcag gactcttttt tattatcaaa catcttccat cttggttaat gccgatcgct
    24181 attgccaatg taatcaatgc agcttcatcg aaagattctt ctcagttgca ggccatttat
    24241 ttcaacgctt ttttgatggc ttgtctgatt ggtcaaaata tattgacgaa ttattttcat
    24301 gtaaaatacc atagcacttc gatccgtcaa gtagaagctg gactaagagc gacccttatc
    24361 aagaaaatcc aagaactatc tattccttat caaaaagaat tgcaatctgg acgaatccag
    24421 tcaaagatca tccgtgatgt agaagctatc caaacgttgt cctctcaagt atttgtcagc
    24481 ggattaaata tcgctgtcaa tttattcgta gctttaggta tcacgcttta taaaagtcct
    24541 gttgttttca tgtttttctt attgactgtt cctctcgccg cattcgttac tctctttttc
    24601 cgtaaaagga tcagacagtc caactcagac ttcaggcaag aaatggaaga gacttctgca
    24661 aaagtgatcg aagtgatcga attagcaccg attgctcgag ctcatgggtt agaaaaacac
    24721 gaaacaactc gcttaaataa gcattttact caaatctatg aaaaaggatt ccacttagat
    24781 atcctgcaat ctgttttcgg atcagtcagc tgggctagtt ttcagatatt tcagctgatt
    24841 tgtttgattt tcactgggat attagcttta caagggcgca tccaacccgg cgatgtcgtg
    24901 atgtatcaga cgtatttcac gacgatcgtc aattccgttt ctggaatgat cacgctgatt
    24961 cctacgattt caaaaggcat ggaatctatc acttctattg gcgaactgtt gattgctggc
    25021 gatgtagaaa cttatcacgg taagaaaaaa atccaacatt tggatggaaa ttttcgtttt
    25081 gaggatgttt cttttcttta tccggactca ctcaaaccga ttatcgatca tttttctcta
    25141 gaggtgaaag ctggagaaac aattgctttt gttgggccat ctggctctgg aaaatctaca
    25201 ctccttaact tattgattgg atttatccag ccgacttcag gaaaaatttt tctagatgat
    25261 cagcctttgg atgaactgga tatgcgaagt gtgcgaaatt atttagccgt tgttcctcaa
    25321 accactttgt tgttctccgc ttcgatcaaa gagaacatca cttatggctt gaaaaacgta
    25381 tcaaaagaac gtttagatga agtcatcgaa gctgcgcaac tttcttcact gattgaccag
    25441 cttcctgatg gtctggatac cttagtaggc gaacatggca acaagctgtc tggtggccaa
    25501 aagcaacgta tctccattgc acgagcgttg atacgtcagc caaaaatcat tttgctagat
    25561 gaagcaacct cagctttaga taaccagtca gagaaaaaaa tccagcaagc cctgaattac
    25621 ctgacagaaa cacccactac ttttattgtg gctcatcggc tttcaacgat caaagaggca
    25681 gataaaatcg ttgtgatcga tgaaggaaaa atcgttgaaa taggtactta cgaaaaactg
    25741 ttagctcaaa aaggctattt ctaccgcttg aaaaatgaat gacaaaaaat aggagctatg
    25801 acaagacttt tgtcgtagct cctatttctg aataaacggt gttctcctgc caactcctcg
    25861 gtcacaagtc acaatagtca tcaaacatga gaagctgttt tttgaaattg ttccttgtaa
    25921 ctcagagtta aacggtaggc tcacgacctc ttggtaaatg gtgttcaaga gacagcctat
    25981 cggcaataag aagcaatttt gtcatcgctc aaggccgaac gtctctttca caccctctgt
    26041 tgtttcataa aatagtttta ttcgctgtaa aggtcgcgaa ctggtgtcat gatgatacgg
    26101 ttcaagtcat tgatgattgt actgaagcct tgctcttttt gcatcaagtc attgattact
    26161 tcttctgatt ggattttcac agccatttct tgtgctttca ttgcatcttc gtcgctgaac
    26221 tcttgtccac tcatttgttt ttcttgtaat tcgatttgca tcgcttggaa gtctttgaac
    26281 agtttgtgtg cttcttcgtt tgcttgtact ttctcgtacg cttctttcaa cgctttgaat
    26341 tctggtaatt cacggatctc acgttcgatt tgattcgcgc tatcgtagat gtttgacatg
    26401 ttatgccctc ttttctttta gtagttactc tctattgtaa catggggtta ttcgttttca
    26461 aaggggctac tctccttgat atttccgcca gaggtcttta gccgcttcac caattccctg
    26521 aacgccttct ttcaccatat cgccgaagtt tttcagccct tcttttgctt gttcaccata
    26581 ttgattcaag ttatcttgcc aatttccaga ctctccatta gaatttcctg tgtttccaac
    26641 ttgatctgca gctaccactt gtcctcctgt ggcataggca tctgctacac taaatcctgt
    26701 ttttgaagta tacggaagga tgccttctgc cgctgatttg aagactttcc cgacaacatt
    26761 tccacttgtc ccttccaaat agtgcagctt gctggattct tcgtaaccca tccatgtcga
    26821 aatcactacg tctggcgtgt aaccgatcat ccattggtca tttgcttttg tggcatcgaa
    26881 attagtttct gtggttcctg ttttccctgc tatcgtataa cctgcaggat tcgcagcagc
    26941 cgctgttcca tttgaaaatg taccaagaag catgctggtc atttcatcgg ctgtttcctt
    27001 tgatatgacc tgttttggtt ttttgtttgt gttatcgacg atcaccgctc cagtcgaatc
    27061 aacgattttc gtgataagat gcggcgtata catctgacca tcattggcaa atgcactgta
    27121 agcacccgcc atgatcaatg gagatactcc tttttccagt ccaccaaggg ctaagccata
    27181 atacttatca gattttgcta aaggcaggcc gaattcttct gctttatcat atcctttttg
    27241 taaaccgatc tcatgcaaaa gccatacagt aggcaaattc aaactttgtg caacagcttg
    27301 atacatcggt acttctcctt ggtatgtccc gtcaaaatta tgcgcatcgt aataactttg
    27361 cggttcgtct ttcaagatac tggaaggctt gtatccagct tccagtgctg gtgtatatac
    27421 gcttaatggt ttgatcgttg atcctggtga acgacgcatc tgtgtagcaa agctgaatcc
    27481 tctaaagaca tgatctcctc ttcgtcctac tagtgcttgt acaccacctg tattcggatc
    27541 taaagctact gaagcagatt ggaccatggc accatctgaa gcatttgctg ggaataatgc
    27601 actgttttga taagtagctt ctaattgctt ttgataatcc tgatctaaag ctgtatagat
    27661 tttatagcct ttattcatga catcttcttc atcaagacca tactcgttaa ctgcttcatc
    27721 gatcactgca tcaaaataat aaggataacg ataaccagaa tctgaattat cgtatgtgtc
    27781 attaagatac gcgctgatat ctgtctggct ttctgcttgt tcttgttctt ttgtgatttt
    27841 tccgttttct tccattacac ttagtactgt gtttttccga tcattcgcat tttttgggtg
    27901 atcgatcgga ttataaatac caggaccttt cagcattcct gccaaagtag cagcctcaga
    27961 cagcgtaaca ttcgttgcat ctactccaaa atactttctg gaagcgtctt ggacacccca
    28021 taccccgttg ccaaaatagg cattgttaag atacatggtc aagatttctt ctttgctgta
    28081 cttcttttcg atctcgattg ccaaaaataa ttccttcgct tttcgatcaa acgtttggtc
    28141 taatgtcaga tacgcatttt tggcaagctg ctgagtgatc gtacttcctc cgccccctga
    28201 tgttccgcca gacaagacca ttcggactgc tgcacgggca atccctttga tatcaaaccc
    28261 gtgatgctcg taaaaattcc tatcttctgt cgagattact gcatcttgga tatagggaga
    28321 aatcgcgttt aattctacat atgttccttt ttgggcgtac agtgtgccag cttcttcgtt
    28381 tttcttatca taaatgactg tcgattggct taatcctgat tttaacgttt cgacatttgc
    28441 ttgttttgct aaaataaaga ggtagatact tgtcactaag acacagatca aaccaatcag
    28501 caaaatcaat ttgttcacat gatatttttt ccagatgcgc tttcgccatc gatggaattg
    28561 tccgagatat ggtctgatcc aacgccaaaa actgatcaaa gcagctttta tcttttctaa
    28621 aatgctttga aaatccattt gcttcttctc ctgtctattt gattcaatcg tcttatttta
    28681 ccatagtgaa agagtgtttt cttcctgctt aagttgtagt tcaatatgtt tttttacgtt
    28741 agtgcaagta gagtaacata agaatgaaca gaaaaatgta agttttttct taaatatgtt
    28801 ttaaaaatgg tacaattagt ctgcgattgc actgaaagga gtacacttgt gaaaatcacg
    28861 atcactttac ctatgaacca tcctgaaaca acaattcgcg acttgcttga aaaggaatgg
    28921 ttagttccta gaaaggttcg acactttttg cgtatacgta aaaacgtttg gatcaatcaa
    28981 caacctgctt tgttccatca tgaagtaaag ggcaatgacc agatcacatt gttattcgaa
    29041 gaaacagatt ataattatca agaagttcaa ttaggagatg cgaagcacat ccgtgtttta
    29101 tatgaagatg agcatttgat tatagtggat aagcctgcag gaatgaagac tcatccaaat
    29161 gaaccgacag aaaacagtac cttactcaat catttagctg cttatctcga cactaaacag
    29221 caacgacctt acgttgtcca ccgcttggac aaagaaacaa gcggtgccgt tttatttgca
    29281 aaaaatccat ttgtcttgcc gatacttgga agaatgttgg aacaaaaaat gatctatcgg
    29341 agatatcaag ccatcgtatg gggagaaatt gccaaagata tgaccatcac caataaaatt
    29401 ggacgagatc gccatgatcg gagaaaaagg gtcgtagatg ataaaaatgg tcagagcgcc
    29461 ttgacacatg tcactgtcaa caaagtactt tctgaacaaa cacaagtcta ttgtgttttg
    29521 gaaaccggac gtacacacca gatcagagtt catttagctc ataagggaca tccgatcgtt
    29581 ggagaccctc tctaccaaaa tagaacagct gaacgactga tgctccatgc attagaactg
    29641 cacatgatcc atccatttac tcaagaaaaa atagtcggta aagctttacc tggattatgg
    29701 gaatagaaat aaaaaagaac agagtcttat cagcagataa tgataagact ctgttctttt
    29761 tgtgtaattt gtctgcttac ttttgatgtt cgtgttctgc taacagcaaa ctaccgataa
    29821 tcccactttc attcggcagt ccccaacgaa cgatatattc acttacttga ggtgtttcca
    29881 tataaccacc catcaattca acaaattgct gacggatttt ttgaaggaga tgatcctgat
    29941 tcattacccc accgcctaaa ataatctttt caggtgctaa cgtcaacgta tagttgatca
    30001 gtgcttgagc aatataataa gcctgaatag cccaaacagg atgatcttgc ggcagatctt
    30061 gccctttgat ccctgtgcgt gcctcaatag acggtcctgc tgctaatcct tctaaacaat
    30121 ctttatgata aggacaagta ccttcataat ggtcatctgg atggcgtttc acccaaatat
    30181 gtcccatttc aggatgtgcg atccctgaaa aaatttcttc attgatcacg acacctgcac
    30241 cgattcctgt tcccacggta aggtaaatac aactattttt tccttgtgct gctccttgct
    30301 tcaattcccc atatgcagca gcattgacat ccgttgtcca agcagcagga atattgtagc
    30361 gttccttgat tgtgccaaga aaatcgaatt gtgcccaccc ttttttggga gttgccaaaa
    30421 cgtatccgta attatctttt gttttgtcga taccaatcgg gccgaatgag ccaatcccga
    30481 ttcccttcaa ttcataacga tcaaaaaatt caaatacctg atccaatgtt tcttctggtg
    30541 ttgtggtagg gatacttaat ttttctaaaa tttctccacc atctgaaacc gcacacacaa
    30601 attttgttcc gcctgcttct attccgccat acattcgttt tcctcctcga atccgattgt
    30661 ccgaatctca taaggttgga ttattgggac aaccgcttgc ttcgttggtt cttcaagcag
    30721 attcaatatg acagtttttc cattgtcttt agttatttcc aatttttcta aatgactaga
    30781 catattgaaa cctcttacta ctactttatc actaaacttg ctgcgtttca aagctgtaat
    30841 tgcaaacgtt tcacttttta tcgttaaata ttgttgtttt gaaatcaatg ttccttcatg
    30901 atgtttgatt tgttgagtgg aaaaaggaat ctgtgctgcg tatgcatgca aataagtaga
    30961 gaatttttct tctggtccat gtagttcaat gctgtaattg aaccgatgct cacctagaca
    31021 ctgtgcttct ggtgttggaa aatatcccca atcgcctagt tctcccacac tgcgaagtag
    31081 cgtcactgcg atttgtccgt cttctgttac ttcatattca ttcaaaccaa agtttccgac
    31141 tgttacaccg tactcttcat catgaatatt gacaaatgct tgctgatgtt gcggattcgt
    31201 ggggttttcc caacttttag acacatggtt tgggcgcttc accacttcaa aaatactatc
    31261 tgcttcgtga tgttccacat gtaactttgt aggaaacaag acacgtaagc gatgatcttt
    31321 catctgattg tcgagagtcg tttcaaagtc gattttttta ctatcttttc tgattgtcat
    31381 gattgttttt atttgaagga ttcgtttttc tttggatcgt tctgcttttc gataacgaaa
    31441 ttccacaacc atctgctgtt ctttttctaa acgctcgtct gcagaaacag ggatttccat
    31501 ttcctgtaga atacttattt ttgctatttc cggtgtatct actaatactt ttacttcaga
    31561 gttttctttg ttactggata ggattggctg atcacagaaa ggttgtttaa agatatactc
    31621 atttcctata tcgccgatat cttcaaaaat atgcagatta ttcaactttc gtttgttttt
    31681 gcgatcttcc attgtaatgg taccattttt ttcaatcgtc aaatgtagag ggccgttttc
    31741 gatgattcgt ccactctgat gaatcattgt ttccttttct tctgctttcg cttccccttc
    31801 tacaagcgcg aatgtttccc aagaaaatgc tggcatgttt tctaatggta aagtcacttt
    31861 gatcattctt gccatatatg gcacacggaa acggtctttc ggcaaatcat aaccaaattg
    31921 gacagtttct tcagataaaa tagctggtat agtggcccca ctttttgttt ctacatgata
    31981 cttacgttta ggcaaatttt ctaactcttg atatagttgt tcaggtattc cttcagcgaa
    32041 tctttttctt tctaattcga tggttatttc tgcttcacca gttttatcca ttcctgcagt
    32101 atttacaatg acaaatggaa agctgtcttt tgggaatccg gtcgtatcga ttgctgcggt
    32161 cagctgttct aaggaatctt gtgcaagata ctttccaact tcatctgctt tttcaaaacg
    32221 aggtatcatt tcccgatgga cagaatcgac tgaacaaccg caaatactgt cgtgaggatg
    32281 attttgcatt aatgtcttcc aagcataatc taattgatca tggggatagt ttccactaac
    32341 ttcataagcc atcgtcgcaa gtggttctgt aatattttct agctgtcgct ggaccttggt
    32401 attccattgt tttaagtaaa cccgagcaga tgctgtattg gctaaagtat accagccatc
    32461 cgtttcttga cttgtcaatt ctccttctac tgagcctaag tcttctggca catctttttg
    32521 aactgcttcc agatagtcag taaaattaga atgaataaac tcgtaatctg gaaacagttc
    32581 atttgcgaga tggattgctt ttgatatatc tttttgtacc ggttgatgat caacaccatt
    32641 catcatcaat aagtggttcg tagaggcata ttgttctgca tccgctaatt tttgcttcca
    32701 aaaggcaaga gctgcttctt tttctactgg aatctcattc ccgttgctat accaattggc
    32761 aaatagcaga ccaaagattg ctgtttgatc aggtcctttc caccacatct ctgagtattg
    32821 ggaagaatag ttttctgctt ccaaaacttg attatcaaag ccaattggtt ttacaccacg
    32881 gccaaacgct gctgctgaaa taccagcttg tttcattaat tgaggcgtct gtcccatatt
    32941 tccaaatgta tcgggaaaat agcctaacat gacaggggtt ccccatttac ggctttcttc
    33001 catgccaatc agcatatttc tcacgttcga ttccgagctg ataagaaaat catcttgcaa
    33061 gatataaaat ggtccaattc tcaattttcc ttcattgatt gcttgttgga ttgcctgtct
    33121 tttttctggc cttacttgaa gatagtcatc taaaataatc gtttgcccat ccaaatgaaa
    33181 actgttgaag gacggatcaa cttcaaatag ctctagtaga tcgtctatca attcaatcag
    33241 tcgcatgtga tgctgttcgt aagccatgta ccattcacga tcccagtggc tatgagaaat
    33301 gatatatact ttctttttca tttacgatac ctcctacttt tctactctaa tatcaaagta
    33361 atccatgact aattcgcaaa acatcatatt tgcccaagaa aaccattctc tcgtataatt
    33421 ttcaggattg ttcacatcga atccttcgtg cataaggtgt gttcctgcgt cagttgctac
    33481 aagtaaattc aatatccgct ctttttcaga tttgtctgac gttgtcattc cttccatagc
    33541 taatgcaatc ggccaaacgt atttttctgg cgtatgggag gatccaattc cttttgcgta
    33601 ctttccttca tagaaatatg gattttcttt actcaacaat gttcttcttg tttgcagata
    33661 atcttcctct tgttcagaac aatatcccaa gtaaggtgcg gcgatcagat tgggcacatt
    33721 actgtcatcc atgactgtgg cgtttcccat tccatctact tcatatgcat aaatcgtttg
    33781 ccctttttga ttaaccgttc gtccgtgatt ctgaatcccc tcttggatct ctttcttcaa
    33841 tttctgtgtt tgctgtacaa tttctttatc atcgagtatc gtttgaaaaa tttcttccac
    33901 ataacctaac acgaccaccg caaacatatt ggatggtacc agatatccat aacggcaagt
    33961 atcatcgcta ggacggaaac cagaccatgt cattcctgta aaagctgttt ttgcacctcg
    34021 cccgtcattg atcagcgtat cttcttttct tgtcgtgtct cgtacaaatg tataaggaga
    34081 gtgttcatga tcttgctctg ttttgaacac gtccaagatt tttttgatac cttcaataaa
    34141 agattgattg aattgatcag tttttcctgt atttttgtaa agaagatagg ccaactgtat
    34201 tgggtagcat aaagaatcaa tttcatattt tctttcccag atccaatcgt tcatttttgt
    34261 atgatctgtt tggtggccag caccgttagc ttcttcgttg aaggcattcg catatggatc
    34321 gatattgata taaaaaaatt gctttttgac taacccgctg atcatgtcag ctaattcctc
    34381 atcttctttt gcaatcacta aataaggacg aacttgtgca gtagagtctc ttaaccacat
    34441 cgctggaatg tctcccgtca ataaaaaggt ggttccgtca ttttgctttt tgactgttgt
    34501 caacagagta ttggcaaatg ctgctgtaaa attctttgcc cagtccgcat ggatatcccc
    34561 acatttttgt gtgatatctt ccataaattt ctgcaccgat tttggtatat ctttataagc
    34621 cactaataaa accttctttc tatttgatat atctttaaag attatacttc atacagccaa
    34681 ttatgtctac gctttcaaaa aatatctttt tatttacttt ttcatccatt atggtattat
    34741 ttgaatatga tatatcatta agaggtgttt atatgaatca accattgtac cagacaatat
    34801 ttaatgattt gcaaacggct attttaactg gtgaattacc tgtagacagc caactaccaa
    34861 ctgaaaaaga actttcacgt acctataaag taagcagaat cacttccaaa cgtgcattga
    34921 ctgaattaga gcaattagga cttatttatc gcgttcgcgg gaaaggcagt ttcgttaaat
    34981 ccgcttctcc ggaacagatt cttgcttctc ctacaaaaac acgacggatt ctgtttcttc
    35041 ttccatatgt cgctgatttg tctgttggtg attttagcaa gggattgaac cctgtgatgc
    35101 aagataaaca gattgatgtc atgatgacga cgcttgactt tctacaaaat aaaacagcaa
    35161 aagagctaat caatgaattt gacggattga tttattatgc tcttgataca gaacaacatc
    35221 ttgacttact ttttgagctt tcattgaaag aattccctgt cgtcatatta gataaaaaaa
    35281 tctatgagtt accttttcct accgttcttt ccgataattt ccaaggcggt gcgcttgctg
    35341 cctcttattt gatcgaacag ggacattcaa aaatcgctta tttgtttggt acgcagactc
    35401 atccacaatc cgttcgacag cgttaccttg gttaccttga agcattggac aaagctggtt
    35461 tgactttcca cacttcactg gatgacaaaa gcgcgacatt agatgaattg ctttcttata
    35521 tcactaagta tgaagtaact gcttttgttt gtgaaaacga tgttgtcgct atccagacca
    35581 tgaatctctt gagacagcat agttatcata tccctgaaga tttttcaatt atcggatttg
    35641 atgatatcca agcagcagct cttgttgacc cgccactgac aaccatcgca caagattttg
    35701 aacagttagg taagcttgcg ggagatgctt tggtcacttg gttagagaca aaagatcgac
    35761 catccgatag taaatttcca gtttcattca tcaaaagaca atcaacaaag gagaaataaa
    35821 atgaatatcg gtcaaataga tactagacat gcaacagcga atcagccaaa tttctctaat
    35881 gggaactgcc agccttatac tggcgttcct tttgggatga attattttgc accgcaaact
    35941 acggatcaaa acggcagctg gtggtttcat cctgatgacc gcgtttttca aggctaccgt
    36001 cttacccatc aaccaagtcc ttggatgggt gatttcagtc atatgctgtt tacgccgatc
    36061 aatggaaaac tacaggagaa cacccttttc catgctcaaa gttcttaccg cccagaagaa
    36121 agtatatttt gcccgactca tctgtcgatc tgtcaattac gttatggcat ctgttctaca
    36181 ttgataccta gcatgtatgg cggaatctta tctattgatt acagcaaaaa tgacagcggt
    36241 ttacttttat cctttccggg acgccatcag ctctttgtta tagatcctta cacagtggaa
    36301 ggaaaaatca cacattttgc tggttcagaa gatcctgatt tttcttttta ttttatattg
    36361 agatttgaat atcctttaac aattgatgag ttagctgttt ccaacaaaaa tagcgactcg
    36421 atcccgttgt attttgaaca gacaaaaaaa caaactatcc gatttgggac atcttttatc
    36481 agctcggaac aagcagctta taacctggaa ctagaacaaa accaaagcaa agagacttac
    36541 cttgctcaca gtcaagaaca atggcagtcc tattttgatc gtatacagct cacacaccat
    36601 gatcaacaac aattgcggac gttttatcat aatttatatc gggtgttttt atttccacaa
    36661 acattttatg aaattgatcc tagtgggcaa aaaatccact atgatacgac tagtcgatct
    36721 ataaaacctg gtgttctcta tacaaataat gggttttggg atacgtataa aacggtttat
    36781 cctctttatt ctttgattgc tgtagaaaaa tacgaagaaa tgctggaagg ttttttgaac
    36841 agctatagag aaagtggcta tttgccaaaa tggctgtctc ctgatgaacg tggacttatg
    36901 cctgggacat tgatcgatgc ggtcattgca gatgctgctg taaaagatat ccgacgagac
    36961 ttgatgcctg aatttctaac ggcaatggaa aaaggtgcta cggttcaaag caaacatcca
    37021 aattatggac gccaaggaac agaagattac ttgacatatg gctatgttcc taatcactac
    37081 cacgaatcag tcaatcacac attagactat gcttatagcg atttttgtat tagccaagtt
    37141 gccaaggtac tagaagaaga ggaaatgacg aagaaatatc agcagcaatc attgaactat
    37201 caaaatatct ttgactatga gtcaggattt atgcgttcaa aagataaaga tggcaagttc
    37261 agagaaccat tttcagctaa tcgttgggga caagattatg cagaaggaag tgcttggcaa
    37321 agcagctttg ctgtttatca cgatttttca ggactgatcg gaaaatatgg tggcagtgag
    37381 agatataaag aaaaactcat tgagctgtgc aatcagcccc caacgtttga cccaggagga
    37441 tatggttttg agatccacga aatgagtgag atggccgcga tagaatttgg acaattagct
    37501 atttcaaacc agccaagttt tcactatcct tacttgttca gttatatagg acaacctgaa
    37561 atggcacagc cactgcttaa acagttgatg actcagacct tcgacgattc tccaatgggt
    37621 taccctggag atgaggacaa tggaagtatg gccgcttggt atatcttcaa tagtctggga
    37681 ttttatcctg tcacgcctgg aaccggtgaa tatgttatcg gtatgccatt aattgacaag
    37741 gctgttttgc ttttgtcaaa tggaaaggaa ttgacgattg aagcaacgcc taatcggccg
    37801 cagcaacaat ttatccataa aatcaaacgg atggacaaag aatatacgaa attatatttt
    37861 acccatcaag atttgctgaa aggcggcacg atctcttacc aattagggat catccctcac
    37921 cctaagaaat atcaagcaaa agatttgcct ttttccttat caaactataa ttaacaagag
    37981 gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caagcaaaa
//
LOCUS       DAZKCZ010000013        36569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000013 DAZKCZ010000000
VERSION     DAZKCZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.013"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8KN_000582"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8KN_000582"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9187665.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8KN_000583"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8KN_000583"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9187666.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8KN_000584"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8KN_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9187667.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8KN_000585"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8KN_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9187668.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8KN_000586"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8KN_000586"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9187669.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8KN_000587"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8KN_000587"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9187670.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8KN_000588"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8KN_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFP9187671.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8KN_000589"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8KN_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFP9187672.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8KN_000590"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8KN_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFP9187673.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8KN_000591"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8KN_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9187674.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8KN_000592"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8KN_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9187675.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8KN_000593"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8KN_000593"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9187676.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG8KN_000594"
     CDS             15402..16376
                     /locus_tag="ACG8KN_000594"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9187677.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG8KN_000595"
     CDS             16546..17946
                     /locus_tag="ACG8KN_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFP9187678.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG8KN_000596"
     CDS             complement(18024..18746)
                     /locus_tag="ACG8KN_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9187679.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG8KN_000597"
     CDS             complement(18825..19331)
                     /locus_tag="ACG8KN_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9187680.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG8KN_000598"
     CDS             19454..21253
                     /locus_tag="ACG8KN_000598"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFP9187681.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACG8KN_000599"
     CDS             complement(21321..22895)
                     /locus_tag="ACG8KN_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9187682.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8KN_000600"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8KN_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9187683.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8KN_000601"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8KN_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9187684.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACG8KN_000602"
     CDS             complement(24672..25310)
                     /locus_tag="ACG8KN_000602"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFP9187685.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8KN_000603"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8KN_000603"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9187686.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8KN_000604"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8KN_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFP9187687.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8KN_000605"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8KN_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFP9187688.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACG8KN_000606"
     CDS             complement(29104..29844)
                     /locus_tag="ACG8KN_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFP9187689.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8KN_000607"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8KN_000607"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9187690.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8KN_000608"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8KN_000608"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9187691.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8KN_000609"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8KN_000609"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9187692.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8KN_000610"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8KN_000610"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9187693.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8KN_000611"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8KN_000611"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9187694.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8KN_000612"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8KN_000612"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9187695.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6706 g        11988 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatac
//
LOCUS       DAZKCZ010000014        36569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000014 DAZKCZ010000000
VERSION     DAZKCZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.014"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            347..667
                     /locus_tag="ACG8KN_000613"
     CDS             347..667
                     /locus_tag="ACG8KN_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFP9187696.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            821..1726
                     /locus_tag="ACG8KN_000614"
     CDS             821..1726
                     /locus_tag="ACG8KN_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187697.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1723..2496
                     /locus_tag="ACG8KN_000615"
     CDS             1723..2496
                     /locus_tag="ACG8KN_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9187698.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2677..4101)
                     /locus_tag="ACG8KN_000616"
     CDS             complement(2677..4101)
                     /locus_tag="ACG8KN_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFP9187699.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4312..4758
                     /gene="nrdI"
                     /locus_tag="ACG8KN_000617"
     CDS             4312..4758
                     /gene="nrdI"
                     /locus_tag="ACG8KN_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9187700.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4823..6805)
                     /locus_tag="ACG8KN_000618"
     CDS             complement(4823..6805)
                     /locus_tag="ACG8KN_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP9187701.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6819..7715)
                     /gene="murQ"
                     /locus_tag="ACG8KN_000619"
     CDS             complement(6819..7715)
                     /gene="murQ"
                     /locus_tag="ACG8KN_000619"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9187702.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7718..8779)
                     /locus_tag="ACG8KN_000620"
     CDS             complement(7718..8779)
                     /locus_tag="ACG8KN_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9187703.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8990..9517)
                     /locus_tag="ACG8KN_000621"
     CDS             complement(8990..9517)
                     /locus_tag="ACG8KN_000621"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9187704.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9584..11086)
                     /gene="eat(A)"
                     /locus_tag="ACG8KN_000622"
     CDS             complement(9584..11086)
                     /gene="eat(A)"
                     /locus_tag="ACG8KN_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9187705.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11344..12465)
                     /locus_tag="ACG8KN_000623"
     CDS             complement(11344..12465)
                     /locus_tag="ACG8KN_000623"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9187706.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12591..13532
                     /locus_tag="ACG8KN_000624"
     CDS             12591..13532
                     /locus_tag="ACG8KN_000624"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9187707.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13682..14578)
                     /locus_tag="ACG8KN_000625"
     CDS             complement(13682..14578)
                     /locus_tag="ACG8KN_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9187708.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14847..16187
                     /locus_tag="ACG8KN_000626"
     CDS             14847..16187
                     /locus_tag="ACG8KN_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9187709.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16405..16848
                     /locus_tag="ACG8KN_000627"
     CDS             16405..16848
                     /locus_tag="ACG8KN_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP9187710.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17352..18770)
                     /gene="nhaC"
                     /locus_tag="ACG8KN_000628"
     CDS             complement(17352..18770)
                     /gene="nhaC"
                     /locus_tag="ACG8KN_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9187711.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19173..20396)
                     /locus_tag="ACG8KN_000629"
     CDS             complement(19173..20396)
                     /locus_tag="ACG8KN_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9187712.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFLYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20427..21266)
                     /locus_tag="ACG8KN_000630"
     CDS             complement(20427..21266)
                     /locus_tag="ACG8KN_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9187713.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21283..21963)
                     /locus_tag="ACG8KN_000631"
     CDS             complement(21283..21963)
                     /locus_tag="ACG8KN_000631"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9187714.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21965..23038)
                     /locus_tag="ACG8KN_000632"
     CDS             complement(21965..23038)
                     /locus_tag="ACG8KN_000632"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9187715.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23094..23332)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23375..23608)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23685..24356)
                     /locus_tag="ACG8KN_000633"
     CDS             complement(23685..24356)
                     /locus_tag="ACG8KN_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9187716.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24443..27091)
                     /gene="mgtA"
                     /locus_tag="ACG8KN_000634"
     CDS             complement(24443..27091)
                     /gene="mgtA"
                     /locus_tag="ACG8KN_000634"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9187717.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27433..27607)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27780..28217
                     /gene="ndk"
                     /locus_tag="ACG8KN_000635"
     CDS             27780..28217
                     /gene="ndk"
                     /locus_tag="ACG8KN_000635"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9187718.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28239..29105)
                     /locus_tag="ACG8KN_000636"
     CDS             complement(28239..29105)
                     /locus_tag="ACG8KN_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9187719.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29265..31358)
                     /locus_tag="ACG8KN_000637"
     CDS             complement(29265..31358)
                     /locus_tag="ACG8KN_000637"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9187720.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32107..32730)
                     /locus_tag="ACG8KN_000638"
     CDS             complement(32107..32730)
                     /locus_tag="ACG8KN_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187721.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32889..34286)
                     /locus_tag="ACG8KN_000639"
     CDS             complement(32889..34286)
                     /locus_tag="ACG8KN_000639"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9187722.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34318..35289)
                     /locus_tag="ACG8KN_000640"
     CDS             complement(34318..35289)
                     /locus_tag="ACG8KN_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9187723.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35663..36088
                     /locus_tag="ACG8KN_000641"
     CDS             35663..36088
                     /locus_tag="ACG8KN_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187724.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        10575 a         7614 c         6405 g        11975 t
ORIGIN      
        1 tatcttactt attgtaaggt aaagcttttt ttaatttatt gcccgataat atatagaaat
       61 aaggtctagt tactgttttt tttaaatgat taataaataa caacttcttt ctatgaaatt
      121 gcaaagcatt catcttcttt tccgctaacg atatatccgt cagtgcttgg tacactttac
      181 taaaaagtat gagattgccg atatctggat aggtgtgcta tactacagaa aaagatatac
      241 aatctgccac cgggtaggat aaagaggatg atcattccat taggtctttg aagggatgat
      301 tttcctcttt ttgtgtattt ttttatagtt aaggaggaat gtaagtatgg catggatgga
      361 attaatcata gcaggaattt tggaagtatt ctggtcaacg atgatgaaat ggagtgatgg
      421 tttttctaaa atcaattatt ctttttacac agtgattggc atgattgcta gtttttactt
      481 tttgtcaaag gcaatcaaaa gtttgccgat gagtctctct tatcctatct ggacagggat
      541 tggcgcagtt ggttcggtca taattggtgt ggtcttgttt catgataagc tgaatctttc
      601 cacttggttc tttgtgggga tgctactcat cagtatcatt ggcatcaaga ttactagcgg
      661 ccactagaca cttggaataa gtactgatca gaaaaaataa gcctttttat ttaatattca
      721 atttttttat gaaaaaataa gaaataagtc caatgaccta gcgacttctc cactcatcag
      781 gtgtatgctt gtactatcta tagaaaagga gtgaatattt ttgaaaagtc tttcggtcca
      841 agaattatcc aagaactttg gtcacaaaca agccctcagc gatgtcagtt ttagcttatc
      901 ccctggcaaa atcgttggat tagtaggtcc taatggcgct ggaaaaacaa cgatcatgaa
      961 agctatttta ggactgatcc attactcaga tggaacaatc tgtattggtg aaaatgagat
     1021 ttctccctct agtcataaag ggttggaaca agtaggagca ttgatcgaat acccgggtat
     1081 ctatccattc ttaacaggct atgatcatct gaagttattc tctgaaacca acgatgtatc
     1141 tgctatcgac actatcgtga atcagctcaa gatggaaaag tacattcaca aaaaagcgaa
     1201 atcctactcg ttgggaatga aacaaaaatt gggaattgct cttgccttac tgaaccatcc
     1261 gtctatcgtt attttagatg agccgatgaa cggtttggat ccacaagcaa caagagatgt
     1321 gcgaaaaatc attaccgact tggctactca gggcacctct ttcttgatct ccagtcactt
     1381 actcagcgag cttgaaaaaa tggtcgatga gctgatcctg atcaatcaag gaaagatcat
     1441 caaacaatgt acaatggctg aattgaacca aacagcgcaa gatttcatcg ttatctctac
     1501 aacaaatgat ctagctgctc gaacgatctt gacagaagct ggatatactt tggaagatcc
     1561 tgataaaatc aagcttttaa aagaaaacga agaattatta ggaaccgtga tccagctatt
     1621 gtctgcacaa aatatccatg tgacagacgt ccagcatctg acgaatgatt tggaatcttc
     1681 tctattggaa attctggatt cagtaaaggg ggaagaagaa aaatgacatt gcttgtaaaa
     1741 caagaattat ttaaattgat caagaaaaag tcaactgctg ttttatctgt tctattagtt
     1801 gttttgctca taggaacagc acttctagcc aaaaaataca cgacgatcat tgatccagtt
     1861 gaaatgactg ctcagctttt tagtgccaca tcttggattg tctttatcat gattgcagct
     1921 gcaagcacga tcatttcgat ggaagcacaa tatggtacat tgaaaaatct cctttatcga
     1981 aagtattctc gaggagaaat attagtaagc aaatggatca cattagttat ttattctgtt
     2041 tatttatacc ttttagctat tattgtcact gtgttgatga aactgattct tttcccttcg
     2101 atttccttca cggagaaggt ttctactggt caaacactga tccaaagctt gttcacttat
     2161 actttgggta gttatatcgg tttatggctg attttaagtt tagtgttgat gattgcttgc
     2221 tttatcaata gttccggcgc atccatctca gcaggaatcg tcttttattt tgcttcgtca
     2281 atcatatctg gtatccttat tgcactcatc caaaaatggg agtggatcaa atggaatccg
     2341 atcagtatgt tgaatctgca aaaccaaatt ggaaacgaag agatcatgaa gcaattgact
     2401 catctttcta ctaatcaaat gctattcggt aatttagctt acatcgtttt gttcttagca
     2461 ttaggatatc tcgtatttaa gcgaaaaaat gtatgatgac tcatcaaaaa agaggctgtg
     2521 aaagagacgt tcagccttat tgccgattgg ctgtctctta aacaccgttt attctgaaaa
     2581 aggagctgtg acaacatttg tgtcacagct cctttcctag tatgtttttg aataaacgac
     2641 aggctcacaa cctctcttta tacttctttt atacttctat acgtgaagca tcccagtggc
     2701 aaataaaacc attgcaatca ctccgaccat cgtcacacct ataagtaaaa tcttatctaa
     2761 gtggatatgt tgtacaggct cgttttgctc cttacgtgct ttgaggtaaa aataaatgcc
     2821 tggcaagaaa agaacaagaa caagcattac ttcattaaag gctacgacta gcatagccca
     2881 tacttgaaag gcacttgcta aaagccctac taaaaattgg acccattctt tccgctgata
     2941 agaaagtttc atttggtaca atcctaagaa aagccacgtg aagaaaatag ctgctgtacc
     3001 aagtgtgtaa gcaaagttat aagcactgtc agtgaataat aatgaaagta aaaagacatt
     3061 cgtacataac gtcgtcaata cgatagcata agttggtgcc ccatttttgt tgagttttcc
     3121 ccatgcttta ggcaaaagtc cgtcttcagc catgtttccc attgtttcaa tcggtaagat
     3181 cgtccatgag agccaaacac caactaaaga aatgatcaaa ccgccgttga tcacgactgc
     3241 tccccattta ccaaccatca tttcaaacac atagcccata gcaggctgcg ccttgatcgt
     3301 ttgcagctga ttcatcgtca ttgtcccata tggaagcatg gaaaccaaga cgtaaataat
     3361 aatcaagccg aatatcccta gaatcgttgc tttcccaaca tctgattgtt tttttgctcg
     3421 tgaacctacg acagaggctc cttcaatacc aacaaatacc caaagcatca ccatgatgca
     3481 actcaatact tgactggaaa acgggatcgg ctgtccattc gcattcaata taatgtcgct
     3541 agaaaagtta tcaatgaatt gttggatcga aaacgtgctg atagctacta caataaataa
     3601 aaataatggc actaatttac agaatgtgac gatagcattg acgaaactag cattttcgac
     3661 tccccgattc actagccatg catatcccca taaaaagact gtactcatca cgatcgatgc
     3721 cagattttgc ccaccttcaa atactgggaa gaaatatcct aatgcgctca tcaacaatgt
     3781 tgcaaaagct acgttgccta accagcatgt gagccagtaa ccccaaccgc tgataaaacc
     3841 agcgaaacgg ccaaaacctt cttctgcgta actaaagatc ccgcctttta aatctggacg
     3901 ttttttattg atattattga tcgacaagga gagcatcaac attgctgttc ctacgagtac
     3961 ccaactgaaa atagctgggc cgccagccga tccggagatg tccgtcataa taccaaatac
     4021 gccacctcca atcgctgaac caattactaa ggcagcaagt ggaattaaac caattccttt
     4081 tttctgttgc tctgtatcca tgtaagcatc ctccaaaata aaaccgcttt tatcaaaaag
     4141 tataacaaac cttttttatt tttacttaat taataaaaac tttaatagaa atataattct
     4201 accggctttt ttctcattat acaagcaaag cgcctagtcc ctttccaaat tacaccacca
     4261 aaaaacttaa aaaagatgct atactaaaag gaaagaattg gaggatgatc catgaacatt
     4321 ttatatatat cgatttcagg aaatacgcgt gcctttgcca aacatctagc agaatatgct
     4381 gaaaaaatgc atgcagaaga tcctgtcaat cctgaagtta ctttaaaaga aatccatgaa
     4441 aatagtgatt ttacgaaaga aacagaacca ttttttactt tcgttcctac ttatttagac
     4501 ggcggaaatg gtttagataa cggtgataca gaaatcttga ccgaaacgat gcgtgaatat
     4561 ctggaacatg aagacaatca taaactttgt cttggtgtag tcggtagcgg aaataaaaac
     4621 ttcaataatc agtattgtct aacagccaaa caatatgcac aaacatttgg ctttcctttc
     4681 ttggcagatt atgaattacg gggaacacca tctgatgtga cacgaattta tcagatttta
     4741 gcagaaaaca ataaataata aaaaagaaga agctgaaaaa tagatttcag tttcttcttt
     4801 cgtttctatt gtttctatca tattaagctt tgacatccat cacaggtgct tttgcttttg
     4861 ctttacctgt tttttccaag acaaagtttt ttacacgatc catattggtt accaagacga
     4921 tcatggatgt ttctttgcct gctgctttga tcgcttctaa gtcagcttct gctacaagcg
     4981 tatcaggtgt cacttgctgc ccttctgtta ctttcacatc aaatggcgta ccgcctaagt
     5041 caactgtgtt gattcccata tgaagcaaga tttctaaacc atttggcgtt gtgatcccaa
     5101 tagcgtgttt cgttgggaag acagagctga ctaccccagt aactggagaa tagatatgtc
     5161 cgttttctgg ttctacaaag tagccttcac ccatcatctt ttgtgcaaaa actggatcac
     5221 ttgctttatc taaagaaaca actgttccgt cagcaactga tgagaggata gtcacacttt
     5281 cagctgaagt ttgaggtact actttttcag gcacttcatt tactgtggct gtttcagtcg
     5341 ctaattcttc tttttctaaa cggtctttag gaataccgaa gaagaatgta gcaacgaagc
     5401 caccagcata agctgccaat aatccaagga tgtagcctaa ccatttaccg tcagagatca
     5461 atgggatcaa agcggctcct gaaggtccaa tagcgatagc cccgacattt ccgatcattc
     5521 cgatcactgc cccgccgata cctccaccga tacaagcagt aataaatggg cgtcctagag
     5581 gtaatgttac tccgtaaatc aatggttcgc cgattcctag gatcccgact ggtaaagctc
     5641 ctttgatcat ttctgttaat tctttatctt ttttgcagcg aatccataat gccaatgctg
     5701 cacccacttg tcctgcacca gccatcgcaa gaattggaag caatagcgta cgaccagttt
     5761 catcaatcat ttgcacatgg attggagtca aaatttgatg cagtccgaac ataaccatcg
     5821 gcaagaataa tgtgcctaga acgaagccgg caaatgctcc accaacgcca agtacccagt
     5881 tgatcccacc gaccatgcca tcagaaatga aacctgctaa aggcatgatg aagaaaattt
     5941 caatcagacc gatggcaatc aatgaaagag ttggtgtaac gatgatatct accgcatcag
     6001 gtactacttt atgcagtttt ttctcaacga ttgacaatag ccaaacagcg aagatgacac
     6061 ctaggatccc accttgtcca gctgcaagag cttcaccagt aaataagttt ttcagtggtg
     6121 cttccggatt catccctgtc agcatcacaa cggccccgat aactccacca agagttggtg
     6181 ttgcaccaaa aacttgtgca gcattgatac ctgtatagat aactaggtag ctaaacatcc
     6241 cgtttttcaa gatgttcatc acatcgatat attgttgcca tgttgctgca tcaagtgttc
     6301 cggctgtgac taagtttgct aaaactgcgg caatccctgc cacgatccca gtaccgacaa
     6361 atgcaggaat cattggcaca aagatattag aaatgtcttt taataccgat ttgattttag
     6421 atggtttttg tttcgatttt tgtgccgctt tcatttcttg tgcccgttcg tttacacggt
     6481 cttttccaga tgctgatgaa tttgctgtag ctgtacttac agaagaaagt ttttctccta
     6541 atttcacgcc tgccaaatca accatttgct gtgctacttt gttcactttc ccaggtccga
     6601 tgatgacttg aagctgttca tcttctacta caccaagcac tcctggaatc gcttttaatt
     6661 cagagacatt tacaagatca tcattgatga tcttcatacg cacacgagtc atgcagtgga
     6721 caatgctttc tacattgccc atgccgcctg cccctttata aatttgtttt gccagttctt
     6781 ccaaagtcag ttcgtttttt gccatggtct tttccccctc ataaggtccc ttttacaaat
     6841 ccgtttgctt cagctaattt tgcttgagct tcctctttat cactatttgt caaaatcatg
     6901 acgatagcca gcttaacatt ttgatctgct tcttcaaagt acttttcagc tgtttcgtaa
     6961 tcagcttctg tcgcttgcat gatgattcgt tttgagcgtt cgattagttt ttcatttgtt
     7021 ggtttgacat cgaccatcaa gttattgaat actttaccta taccaatcat actgatagta
     7081 gaaatcatat tcaagataag tttttgtgct gttcctgact tcaatcgagt agagcctgtc
     7141 aaaaattctg gacctgcatc gacttcgatt gggaaatccg cgtgtttact gatttcggct
     7201 tgtttattgc aagaaatcgt tcctgtgaca gcaccgatcg attttgcgta atctaatcca
     7261 ccgatcacat atggtgtacg accgctagct gcgataccaa taactatatc attttttgtc
     7321 aaattcagct tgattagatc ttcttgccct agctcataag aatcttcggc cccttcgaca
     7381 gctacagtca ttgcttcttc tccaccagca attagaccta tgaccatttc cggttctaca
     7441 ccgaatgtcg gtacgcattc agcagcatct aacacaccta gtcttccact tgttccggct
     7501 cccatataaa tcagacgtcc acctttttta aaggactcaa tcgtgttagc aatcactggt
     7561 tcgatacttg tcagttcttt ttcaaccgca gcggccactt tctgatcttc tttgttcatt
     7621 agttgaacgg cttgagaaat actcatctca tctaatccaa aagtttcttg gtttctgcgt
     7681 tccgttgtta ggttctctaa attgattttg ctcattttta tcttccttct tttcttataa
     7741 attgatagtt cataccagct ttgatttggt tgatcaatgg tagctcttct ttgataactt
     7801 tagctactac attcactttt tcatccgctg gcaaatcata tttggttaat tggatctcac
     7861 ccatatagcg caaatacttt tcgttatcta aagtaataga ccctttcata cgcgtagctg
     7921 tatttcttgc tgggataggc ggaatctctc gaaaacgtgc atctacgctt cgtatgacat
     7981 ctcttgcatc gtcttgccga ttcgtatgct tccctaatat atattcgtaa aattcttcat
     8041 ctacaggttc cgtatggagc aagactgtct gttctttttg gaaagaagca aattgctcct
     8101 gaacgttttc tgacagacct gcatctccga tgtaaacaat atccgtgtta cttgctaata
     8161 gatctagagc gcctgcaaga ggatggatcc ctcgatgttc ttccaaagtt ggcaatcctt
     8221 gatatagtgg gcctctcaac tctgtatctc ctggtacaaa accttggaca aggagacctt
     8281 gaattttgag ccattgattt tttttctgat accattcttt gtccagccct gtttcgggac
     8341 gtggataata attatgccaa gcttcaatct gtgagaaatc agcttctgct tctttcaatt
     8401 catcgatatc tttaggcgtg atcgtgctag cattcaaact gattttcaat tgatgtgacc
     8461 agtcggcaat ctgctgattg gaaatatgat aatccattct taaacctgtt acaccaattg
     8521 ctttcaaagg cgttagatct tctaatgaaa aacccgctcg ttccaatgcc tctcctgaaa
     8581 tatcgaccat caattctagt ttattctttt tcgcttcgct tcctagttcg atcaatcgct
     8641 tttgatatgc cgaaacatca tcttctggta tatgcatgga agtgaaaata ccgacaaagc
     8701 cattggatgc catttggcgg atatactgtt tcttctcttc tgacaaagaa tcgttcataa
     8761 atattgaaaa gccaaacata ttatccctct ttttctgtat ccgtcttcat ttctatgact
     8821 agagtatata tctaaagaaa caaaatttca atacttttat taaaataatt aaatttcatt
     8881 tctaaaggtt gagtttatat tatcaaaaga attgtcataa cctaaaaaag gcactgctaa
     8941 gcaaaagcag tgccttgaaa tattttattt aaataacaca aaaacataat tactcataag
     9001 aaaccaattc tgtattatct accatgaatg taaagaaccc ctcattaggc atcggatgaa
     9061 aataagcatt gatcactctt atcaaggcat tatggcaaac taacagatag acattttctt
     9121 cgtcttcgat gcattctttc aataaaggaa cgatccgtgc atacacatct aagactgact
     9181 ccccatttgg gaatcgtaca gcgaacgcta aacgtgcttt ttgaaagttc tcatctttgc
     9241 ttggcagtcc atcataatcc ccaaaatcca tttctaccaa tcgttcatca acggtcagcg
     9301 gcaagctgag acgatcagca actgcttgtg ctgtatctct tgctcgttga agaggggaat
     9361 ggatgatttt cgtgattgga acaggcaaat ctactacctt ttctgctagt ttctgtgcct
     9421 gcatctgtcc tttttcagtc agcggcacat ctgcccgtcc gcaaactaat ccttgtgcgt
     9481 tccaagttgt ttctccgtgt ctcgttacat aaagcatgtg attctctcct tcgttggcaa
     9541 agttcccctt aaccatacct tgttgttctt gagattacaa gggttagatt ctctcaagag
     9601 aaataatctc agtagatact ttatccagga aggtttgatc atgttccact agtaacatgg
     9661 caggtttcac gttcaagatc agttgttcta attgttcttg attgaagaca tccaaataat
     9721 tcaatggttc atcccatgta tatagttcag ctggctgtga taaagattta gccaattcca
     9781 cttttttccg ttggcccata ctcatctgct cgatcttgtt atgaaaaaca tctctttcca
     9841 tcccaagctt tcggaggttg ttcaaaaatg cttggtagtc tacttggttt ttctctgcaa
     9901 attccgccaa cgttcctcga ttgtcttcat aattttggct tgcataacta atgctcagat
     9961 gttttggctg gtatttttct cctataacct tgccgttgaa tgcccccaga agataatgga
    10021 taatggatga ctttcctgca ccgttaggac ctgaaatcgc cacccgctga tgaggctcga
    10081 ttgtaaaatg aattggctcg aataacagat tttcataccc taattgaaga tcttctacgc
    10141 ttaaaagtcg cttatggtga gacgcttggc tattcatcgt caaagaatcg atatattcga
    10201 tatcttttaa tagtttttct ttctcgctga tctgggtctc catccgattc acgattgctt
    10261 tggatcgttt catcgtccgt gcagcatcag cacccactgc tcctttattc actcgtctag
    10321 attcagtatc gatgaatccg acttgtttct ttgttttatc tccttctcgg gaacgagacc
    10381 attcggcttt ttcagctgct gttttcttta gcctactgac ttcttttttc aatttttcat
    10441 tttgagccat ttcaaattca tcacgaagtt ttttctgttc ttcatagata gagaaattcc
    10501 cttgataaag ttccagttga cttttttcga ttgctaaaac atggtccact acttcatcga
    10561 taaatccccg gtcatggctg accacgatga agccttgttt tttctttttc aaataagccg
    10621 ctacttgttt tctaccagag atatccaaat gattcgttgg ttcatcgatt aacgggaaat
    10681 gagtgtcatc cacaaataaa agtgccagta ggacttttgt cttctctccc cccgatagtg
    10741 tgctgaattc tctccataag atttcaggat ctgtttgcat caattggagc tctctttcga
    10801 tttcccatat ctcaaaatcc gtaatatcat ttaacacgta ataggttaaa cgttctttat
    10861 cttttgtctg ttgcgggaaa taggcaaatt cttgctgatg gattacttgc ccttgataag
    10921 gtagtttgtt ttgtagaata ttcagtaaag ttgtctttcc tcgaccgttt cgtccgataa
    10981 gtcctagttt ccattgtgtg tcaaaattta gatttgcttg ttcaaataat aatgtgcctt
    11041 ggctgtcgta gccgaatgtc agatttttta tttcgatttt agacattttc catcacctcg
    11101 taaaaaaaga ccagtcctat tttcagaact ggctaaagag gatgcaaggg ttggatacac
    11161 acaaaaagac ccatacatcg aaaaaaccaa gccaatcccg aaaatctgca cactgcttcc
    11221 ttgtctccac aaggtacagt ctgaatgcag ctttcatcaa tcgacttaat ttttcaatgc
    11281 acggatccct cactttgtcc tatatttgaa attacgatag cataaagaaa atgattttgt
    11341 caactacttc gggaggatgg tgaaaaatct gctaagtgtc tgtcctaaga tttttgtttt
    11401 ttctaatgtt tgcctgtgtt ccattgcttc ttctaaacct attggaccca aatgaatact
    11461 taagacaatt gggagaaaat cagaaagtcc ttctagctct tggtctctac ttccgcaaat
    11521 agcgattatt ggttttcgat atttttttgc aagcactgct acaccgtatg gaacttttcc
    11581 gtggatcgtt tgctgatcga tccgcccttc tcctgtaatg atgacatctg cttgttcgat
    11641 ctttttttcc aatccagtga tttggctgac taaatcgaaa ccagaaacca tcttcgctcc
    11701 caacaaaaaa agggctccac caataccgcc tgctgctcca cttcctggaa tatcatctaa
    11761 atccacctcc cagaaatctt ttatttgctg agcaatcttt cctgcttgtt tatctaagaa
    11821 agctagcgtc gtatctattc ctcctttttg tcttccaaat tgccaagcag ctccttgttg
    11881 acctgtatat ggattggtaa catctgcagc gataatcaat tttactttag aaacaagaac
    11941 gtttcttcga tcaaaatgaa gttttttagt agataataaa ggatttcctt ctgtgtaccc
    12001 ttctaaagaa gcacccaagg attggagtaa tcctagacca ccatcgctac acccgcttcc
    12061 tcctaaagtg acaataattt gttctacttc ttgaagcagt gcatctttaa taagttctcc
    12121 taaggcataa ctggaagcaa gtcggatagt ttgatccgtt ggtttatcaa gtagatctaa
    12181 ccccaatgct tgtgcagatt cgatgactgc ggtgtttttt ccttgccaag ttgtcaataa
    12241 atagctgatt tttcgctgac ggaataagag atccatccca tcaatggtac gccatgttcc
    12301 cctgagcgcg taatagattg cttctgcagt attttctccg ccatctgcaa tcggcacatt
    12361 gatttttctt cctttacgat cttcccagcc atttagaact gcctgattta gttcaaatga
    12421 agaagcactt cctttaaatg aatcaattgc cgccaaaata tccatctgta tccctcactt
    12481 tcttcttaag taagacactt tttttcattt ttttcaaaca aaacaatgct attttcttcc
    12541 atttttctat atactaaaag ggaactcatt gaataaagga gagaaaaaac atgaaaatcg
    12601 caatagctgg ggccggagca atgggcagcc gaatcggatt gatgctacat caaagcggaa
    12661 atgaggtcct cttgatcgat cgttggcctg ctcacattga agccattcgt acaaatgggt
    12721 tgattgctga ctttaacggt aaagaagtgg tcgcaaaact gccaatctat tcaccagaag
    12781 aaatcatcga aagcaacgaa cacgtggact tgatcgtagc attaacaaaa gccaatcaat
    12841 tagatgatat gttttgttcg atccaatcca ttattactga caatacttat gttttgtgtt
    12901 tactaaatgg attaggtcat gaagatgtac tggaaaaata tgtgccaaag aaaaatatct
    12961 tatttgggat cacgatgtgg acagctggtc tagctggtcc cggaaaagtc acattgttag
    13021 gtgatggaga aatcgaacta gagaacttgg aaccagaagg cgaagcattt acaaaaaaag
    13081 ttgtagaagt ctttcaagaa gcaaatttaa atccgatcta tagccataac gttcgttatt
    13141 ccatttggcg aaaagcttgt gtaaatggaa cattgaatgg tttatgcacg attttggatt
    13201 gtaatattgc tgaactaggc gcgcaaaaag cggcagagag catggttcga acaatcgtat
    13261 ctgaatttgc ttctatcgca gcaaaagaag gaatcatact agatcaagaa gaagtgtacc
    13321 aacatattgc ttctacttat gatcctgaca acatcgggtt gcattaccca tctatgtacc
    13381 aagacctgat caaaaaccat cgtttgactg aaatcgacta catcaatggt gccatttggc
    13441 gaaaaggaca aaaatatgat attgctacac catattgtgc ctttttgacc caattagtcc
    13501 atgcaaaaga aggaatcctt ggcgcagaat aaaaggctct ctgatgagaa tcagctgaat
    13561 cagcagctgg ttctcttttt ttaagcccaa taaaagatca aaaagaaaac acaactctca
    13621 tgatgccata agaattgtgc ttgtttgttt cagtacataa tctcttcaaa atgtaggttt
    13681 tttatttgtt ttcaacagag gagaggtcat attcgtattg ctcataaata gtggaatcgt
    13741 tagtgataaa tcgttttgcc caattttctg catcataaaa ttccaatagc tctgatagga
    13801 ttggcttaaa tggtgtatgc agaaattccg gtaattcatc taaatcacgt tctcgaagca
    13861 aaaaaatatt ttcaggacga taaaaatgat aatgagcaat cgcttgatta tccgtcacta
    13921 gtttcttttg aaaatacatg gattcaaatg tccgcaaagt cactccttgc tgaccctccc
    13981 gcaacagttc cagaatcccg cgcgattccc ctgttttttt caggtactct tcataagtaa
    14041 gatatccagg ttgttcattc cctcgatgtg gaagaatgaa atagttggtc gtaacatctc
    14101 tttttataaa ttcctgctta tagctttctg ctcgttcttt tctcccatta ttcgttgcac
    14161 caaaaaatag atccgtcttt ggttcgctag aagaaaaatt catatgtttc gaataaaatg
    14221 aactattatg tttcagccca agttcttttg cttccaaaaa atcaaaatga taaatctcat
    14281 cgattgccgg ttcatcttgt gcctgcttca gcatcgcctc gtgttctgct ttgaagtgat
    14341 tccaaaaaaa taacacaatt tttgcattgg taaatgggcg cacgcgtcga gccatctcca
    14401 tatcaaatcc ataatctaaa aaaacgatca gctgataatt tgccaactgt tgtctagctg
    14461 atttaatgaa ttccgactgt agtaaatctt ttccggacca aacatctaaa gagcggatgg
    14521 aaggcaaaaa ataaagtgat ttattttttg caactagcag tacgtcattt ttgttcatct
    14581 tttccgcctc actttctctg tcactttttc ctatgatagc ctgtttaaat agtaaaaagc
    14641 cagtaaaacg tttcctatat ttgtatgaaa atttaacatt tgtatgaaaa tatgatgaaa
    14701 taaatcattc ttctattaaa attcgcctaa aagttttctg aatagttgta attgtctgaa
    14761 aatttgttat cattagtcat tgttaaatat tgctatttat tcttaatata aattttgatt
    14821 gtgaatttaa aagaaagagg tcatttatgg aagataaaaa attgaaaaaa caacttagct
    14881 ccagacatat caccatgtta gctttaggtg gagcaatcgg tgcaggtcta tttaaaggaa
    14941 gtggcgaagc gattgggatc gccggccctt ctgtattgat tgcttttttg attggtgggc
    15001 tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt ggcgacacgc
    15061 atcacggatt atctggtttg atacgccctt atctaggtgc tcattctgct gattttactg
    15121 attgggtcta ctggtctatg tggattatca atatcatcgc tgaagctgtt gcagctgctt
    15181 cctttttgca gttgtggttc ccgcacattc cagcctgggt atttgttttt atgttggctg
    15241 ttttaactac gatcatcaat ctttattcgg tacggttatt cgcagaaaca gaatattggc
    15301 tggcttttgc taaaatcagt gtcattatct tactgatcat ttttgctact tatcttgtcg
    15361 gtcagcaaat gttgggcaca ggcgttttcc ctacccttca gcaattgact gaccatggtg
    15421 ggtttactcc tcatggtatg aagggaatca tcagttcttt gcttgtagtt atctattctt
    15481 acggcggttc tgaactgatt gctatcacgg taagcgaagc agatgatcct aaacgtgcga
    15541 ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc cttttacatc attcctttgt
    15601 ttttgctttt gattcttttt ccttggaata ctttagcagg cacgaatgtc agtccttttg
    15661 tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat tttgtgattg
    15721 tccttgcttt gttctcatct atcaattcgg gtgtgtatgc atcttcaaga attttgttct
    15781 tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac aagcatcaag
    15841 taccgcaacg tgctgtttta ttttgtacca gtgttcttta tcttggtgta gctctttctt
    15901 attttgtcgg tgacaaatta tttggctatc tggcaggttc cctttcgtat acagtattat
    15961 tgatctggat cattatcagt gcggcatcct ttgtactggc actaagaaaa ggaacattat
    16021 gggataaagg aatcagcttt tttgcattag ctgtcttagg attgattttc cttggtatcc
    16081 tgttcacgaa ctccattggg gtaactgtat tgactggcct gctttacctg ttcatttatt
    16141 tcagttatca aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat cgatagaaaa
    16201 ttttatttta tttatgtgga atagatggag ctattgaaga aaggagttgt gacaaaagtc
    16261 ttgttgcaac tcctttttcc gaataaacag tctcatgacc tcttggtaaa cggtgttcaa
    16321 aaaccctctg tgcaaaataa ttgattgttc tccatgctca tgacatgata gaagaaagga
    16381 atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt gatgatcggt
    16441 gtttcaacgg tagttatgtt tggtatgatg tacttgaatg tctatgcgct cgatcatatc
    16501 ttcttcagtc gtacacgagt attcatggcg ttgatgatgg gtgcagtcat ggcagtcatc
    16561 atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat gatcttagcc
    16621 gctagtcttc ttgtttttgc cggttccttg tttatggtac gaagccaaac tactgtcaac
    16681 gacacagctt ggatgaaggc catgattcct catcattcta tcgctatcct ggcaagtaaa
    16741 cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat tcaggcacaa
    16801 gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc attatccgtc
    16861 caaataaaaa aagaggatgt tccagaagtt atcagcttca agaaataaga agaagctact
    16921 tctggaacgc cgtgaataag gggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    16981 gccaaacaaa aatagcttct cctatttttc gtttggttag gcttattacc gcagatgcaa
    17041 gcttttgaac accgtttatt aagaggttgt aaaatcagcg ttctgacctg agtattagag
    17101 agacaaatgg aaaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca
    17161 gctgattgaa caccgtttat cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag
    17221 aaaaattttg tgaaatggct ttccacattt ttgtataaat ttggcttaat accgagaaac
    17281 tggcttttga ataccgttta ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc
    17341 tttttatgtt tttattgttc taattgcgct gtcttgctta ctgcttcttg cggtttcaca
    17401 aatttttttg agattcccca acctgtgaag gcactgacca tcgaaaacac aggagaaagc
    17461 aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa taccaaatgt acttgctacg
    17521 aaacttccgg caattcccca aggcactaag tagttgatta ctgttccacc atcttctagc
    17581 gttctaccta atacagttgg atcaaatcct gctttttcat aagtttcttt aaatgcatta
    17641 ccaggcagaa taacagatag gaattgttca ccaataaaaa tatttactcc gatacttgtc
    17701 atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct ttcttgatac tcgttccatc
    17761 acggttgaga tcaagccaaa ttcgatcaac aatccaccta atgacaaagt tagaataatc
    17821 aatgacacag taggcatcat actttcgatt cctccgcggc taagcagtga atcgatttgt
    17881 tggtttccag tatttgaaac aaaccctgat tcgatgaccc cagccaactt cgttaaggaa
    17941 gtttgtgggt tttgaataaa aatcattcct actgctacag cgatattcaa caaaatcgtt
    18001 aaaattgccg gcaccttttt ccatgcacac agtaacatta aaaataaagg aatcagtgac
    18061 cagattgaaa tagtaaagtt tgcttctaga acatcagtca cttcacgaat agatgaaaga
    18121 cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc
    18181 gggacagttg accacatcat atgcttgatg tgtttgaaca aatcggcatc aacaatagct
    18241 gctgctagat ttgttgattc ggataatgga gacatcttat ctccaaaaac ggcacctgaa
    18301 acaatagctc caacaaccag cgctggattt aagcctagtg ttgtcccaat accgaaaaat
    18361 gcaatcccaa tcgttgacat aacagtaaag gcactcccta cagcgctacc tacgattgca
    18421 cacacaacaa atacagaagg gacaaaccac tggacactga tcaatttgaa cccaatgacc
    18481 attagagttg gaatgatccc tgcttgtatc catacagcaa tcaatgaacc gactaaaata
    18541 aaaataaaga ttgggatgat tcctggtttg atgccattga tgattccttc gtgcagccaa
    18601 tctgtcgggt atctttttat cactgcatac aacatgacca ttccgatcgc tgctaagatc
    18661 gtgatattag gtgagacctt taatccgaca acgccaatcg caatgcctaa aacaataacc
    18721 attaatacaa gtgttgcttc taaaaaactt ggtgttcctt tttttcccat gataatctct
    18781 ccttttttag ttgttcttat gttctaaaaa agttaagata tcggattact ccgccgcaat
    18841 tcatccgcga ttcctccttt ataaaaataa aaagacctgt tttaaactaa ggacgaatga
    18901 ctccgcggta ccaccttaat taaaaacaga tctgtttttc actttcaaac ttttattcaa
    18961 atgtatggac tttccatgta attcgtcaat taggcttgat cggttcacag cgaccaccga
    19021 ctttctgaac aaaacaccta accgctactg cgtgaaaagt tgacttgtac gaagtttacc
    19081 ttccataaga tcatttgtca atgaaaaaga aaatattttt tcatttttct gaaaattatt
    19141 tttataactt tcacacacaa gtttattttt tactagaaag tagcgccctc ttgttttttt
    19201 cttgctacta tttcttgatg ttcttgcttg attctttcta atagctttgg ctctttcaac
    19261 agatcaagag ctgtattggc gagtagttct gctgcaatcg caatagaagc aaggcctttg
    19321 tcacttttag cagcagcttt gaattcttct gtgtgtccgg caatatatgt ctcagagatt
    19381 gctaccgtag gctggattgt tggtatgact tggctgacat ttcctacatc agaagaaccg
    19441 agacttgttc tttgttccgt ttctatttct tcatctggaa cacctgcttc ttttgcatgc
    19501 tggaagaata agtcatcaaa agaaggagta acgatgacat catcaactgc attttggaac
    19561 aatccaaact caaaagtcgt gcccgtactt atcgcagcac ctcgtacgat attttccact
    19621 ttttcatata ctttatctaa tgtttgccga tttcctgcac gtaaatagaa acgtccagca
    19681 gcgtaatctg gaatgacgtt ggcagctact cctccgtctg taatgatccc atggatattg
    19741 acatcttttg gcaattgcaa gcgtagagcg ttgattccat taaacacttg gatcaatgct
    19801 tccaaagcat ttactccttt ttcaggtgct gctgctgcat gggaagcgac accgtaaaat
    19861 ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt tcccataaag aaaagctgga
    19921 tggacgcata acgctacatc gacatcctca aaaaaacctt ctcgaacaaa gctgccttta
    19981 gctgatccgt tctcgccccc ttcttcccct ggggtgccat atacacggat ttctccacca
    20041 gtttcatcaa tgaattgctt cactatgctg gcggctaata cggagtaagt accaaacaaa
    20101 ttatgcccgc atgcatgacc aatacctggt aaagcatcga attctgccaa aaaggcaatt
    20161 gtgggaccgg gtttattgga aatataacgt gcatcaaagg ctgttcggtg tccagctact
    20221 tctttttcta cccaaaacct ttctttttct aattgtttaa tcaacgctgc tgaggaataa
    20281 aactcatagt tacttacttc aggatgttcg tggatatcta gagcaagtgc ttgatattct
    20341 ggtaaacgat attggattag atccttcaat tggtttgttg agattgttgt tgtcataaaa
    20401 aattcctcct atgctagtca tttttcttat tttctcccga atgtttccca tgcaggcacg
    20461 cttgatcctt ttgatgtttt ctcgattact ttgactgtgt cttctgtttg ataagcgtct
    20521 actatttttt gatacgtttt attgttttca tcttcttttc ttgcaacgat gatattcaca
    20581 taaggacgtg catggtcatc cactggttct aagaagatgg catcagaagc aggattaaat
    20641 ccagcatcta cagctacacc gctattgatc actgaaatgt ctacatctga caaagcacgt
    20701 gctgtttggg aagcatctaa ttcagaaatg tctagatttt ttctattctc ggtaatatca
    20761 gaaacagtgg gcgcttgtcc ttttgcctta tccactttga tcaatccagc agtttgcaat
    20821 aacagaagag ctctgccgcc atttgtaaca tcattaggga ttgctacttt tgctccgtct
    20881 ttgatatcag ctacatcctt caaagtgtta gaataaattc ccagcggtgc attgacagta
    20941 tttccgatcg atactaaatc agttccgtgt tctttattgt aattgtctaa aaagaattga
    21001 tgttggaaag aattcaaatc aatcgatcca tctgctaaag ccacatttgg ttgattgtaa
    21061 tctgtaaatt tcacgtattc caaattaatg ttctcctttt tcaaacggtc tttcacgttg
    21121 tcccatacat ctgtatcttc tccaataatc cctagtttta cgatagtttc ttttttgtca
    21181 gttgtatctg cttttccttg gctacaccct gccaaccaaa taattgctac aaatgtaatg
    21241 actacaaaac gaataatttt tttcattttt tctcctccac tttcaatgtg taattttttt
    21301 aatcagttga ttactgataa attgacttaa aaaaactaag attagaatca ataatgtagc
    21361 cacaatcgtt acatctgttt ggaatcgatt ataccctctc gatatagcaa gattccctaa
    21421 tccgcctgca cctactgctc cagccatggt agtcagtccg atcacgttga tgatagtcaa
    21481 cgccgagacc cgaatgatac caggcaaacc ttctaacaaa tacacacgta aaatgatacc
    21541 gattggactg gttcccatcg cttctgccgc ttcgatcacc ccaggatcaa cttctagcag
    21601 tgcgttctcg atttgacgag caaaaaacgg aacaactcca aaaatcaatg gcactaaagc
    21661 tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta gtcaatgcta aaagagaaag
    21721 caagataatg aaagggatgg aacgaatgat attgatcagc ttatcaagta tttcaaatag
    21781 cagacggtta gctagtaatc cattagggcg tgtcactact aacgtcacac ctaagatgat
    21841 tccaatcatc ccgccaatga atgctgtcca aaaagacata tatagtgttt gcttcgtcgc
    21901 ttcgacaaat tcatccggaa tcaaataaac attgggcaaa tatgttttaa agaaatcagt
    21961 cattttatct tcctccttcg atcactttga tacgactatc taaatcgttt ttttgtaaaa
    22021 tatttatttt tacaccagat ttgatcaaat aatccaatgc ttttttccgc tgtttggttt
    22081 ctccagctaa agtaacgatc aaactcccta acggcgtttc ttgtaataac tctacattgc
    22141 catataaaat attcgctgtt acttgatatt tgctgtacag atgagcgatt agaggttcgt
    22201 tcgtctgatt tccaatataa cttaattcca ccagccattt attggcgatc tgatcggcaa
    22261 aagcagaatg ggcaatgatc gtttctaatg cttggtcaag atgagtagct gttcgaataa
    22321 aatctttggt caattcttct tcaggattgc tgaagatctg gatgctgcta cctttttcaa
    22381 cgattcttcc atcttccata accgctacct tgttgcagat ttctttgaca acttgcatct
    22441 catgagtgat caggacgatt gttaaaccta actggcgatt caattttttt aacaatgcaa
    22501 ggatctgaag ggtcgttttc ggatcaagtg cactggttgc ttcatcacat aataatacct
    22561 taggttcatt cgccaaagct cgtgcgattg ctactcgttg tttttgtccc cccgacagtt
    22621 gtttaggata agcagatgct ttttcttcca aaccgacaag ttccagtagt tcattcactt
    22681 tttgacgacg ttcctgtttc gattttccag aatactttaa agaaaaatca acgttgtcaa
    22741 aaatagttcg gctttccatt agattgaaat gctgaaagat cataccgatg gcctttcttt
    22801 cttttcgcaa ttctttggga gagagctgac tcagattcgt ctgattgaca atgacttctc
    22861 ctgctgttgg tttctgcagc aaattcatca cacgaactaa cgtacttttc cctgcacctg
    22921 agtaaccgac gattccataa acatcgcctt tttcaatcgt taaatctaca gaatctactg
    22981 cctgtatctc tttttctttt tgtttgaaac atacagaaac atctcgcaat tcaatcatta
    23041 aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg
    23101 acgctttttt gcgtgttacc acctcatgtt caagctgtgt tcacacacaa ctcctcttca
    23161 agtacgatca tactctagca agatatcggt cgcagccgtc atcgccttac atctatgctc
    23221 gggatgaatt gctcgatggc cattttccaa tagtcgttcc ttgcttcttc tcagctaccg
    23281 aagctctctg tcaaaggttc ttctatcgta cttaccattt caatgcaaat aatattcaaa
    23341 aaaaaagtcc ttttactaac ataaactatg tcagtaaaag gacgacgtat cgcggtacca
    23401 ccttttttcg gaagtttcct tccctcaagc ccaacatctc taaaagagag attggcgttc
    23461 gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg acaaattatt ctccaaggcc
    23521 attttcagct tttcttactt gactcttctc accagccaga gcctctctgc aaagtttcca
    23581 aacttacttt ccttttcaac gaaatcgata ttttgttgtt gggattaatc tacatgagga
    23641 aaactgaaaa gtcaagtacg aaattcaaat tattttttct atatttattt ttctgttttt
    23701 ttatttaaca tttcaataac agttcgaaga tcttttcctt ccgcaagaag tttcaatttt
    23761 tccttttctt cgacggtata atttcggaca aaactggaat caggtatcaa aacaacgcct
    23821 aatcctgctc gtgaagcagc tgttgctcct actaaagaat cttccactgc aaaggctgaa
    23881 ctagcttcta atcctgttcg ttccaaagct gtcagataag gaatcggtga aggcttgttc
    23941 tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc
    24001 aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt
    24061 tcttttaagt ctgccaatat ttcttttgca tatggtttta agtggatggc tccgcttgtc
    24121 agctgttgat aaaaatattc ttcacgcttc gctcggactt cctgtacttt ttcagggcta
    24181 cctgctgctt tcactaaaat atcaaaactt tgtttccagt tttttcctcc ccaagcgtca
    24241 acgatttctt ttgggatatg gatatcaaat tgctgaaatc cccaaagcca gccatcacga
    24301 tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa atattgcacc tttcatctat
    24361 acattaatcc tttccaaaac aatacacata gtttagcacg actggtccaa aaagaacaga
    24421 agcaaagaac cagccttctc gtttataaga gttcttggaa tcgacggata tagattttct
    24481 tcacagctgt cacaagcgcc agataagcga tgactgtagc aaataaccat aaccagtaat
    24541 tgccaggaag tggcatcaag cctaattcag caccaaaact tgtaaaagga aggacagaac
    24601 cgacaataat tcctaatgta gtgataacaa acatggcaaa tgttgcgttg ctttggataa
    24661 atggtacttt cggtgtacgc agtgcatgga gcactaaggt ttgtgtccat agtgattcga
    24721 caaaccatcc tgcatggaat aatgcgatga aaatcactcg ctgtgaacta tccaatgtat
    24781 ggaaacttcc gcccactaca gcaggacaaa taacaaaata catcaacaag taagtagtaa
    24841 tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa ttccccaata ctagatgcat
    24901 tccatttttt tggctcgtcc aaatattctt tatccatccg atcccatggc aaggaaatgc
    24961 aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg
    25021 gcaggaacgt acttgcaatc agcacggaaa acatattgcc aaaattagaa cttgctgtag
    25081 ccttgatata tttcatgata ttcccaaaag tttctcgtcc agaaagaagg cctctttcca
    25141 aaatcgtcaa atctttttct aaaaggatca cgtctgcaga ttctttcgca atatccacag
    25201 ctgtatctac agaaataccg atatcagcca ctttcattgc aggagcgtca ttgataccat
    25261 ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt
    25321 gctgaggatt caattttacg aatacttcat ggtcttcagc tacttgtgct aattcatgat
    25381 cactcatttc cgagattttc tcaccggtaa ccaattcatt gatctctaga cctacttgac
    25441 ggcacactga acgtgtaact aattcgttgt ccccagtcag aacttttaca gctacaccat
    25501 gttctttcag agctttcaac gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa
    25561 gataaccaat caaaaccatg tctgactcat ctttgactga aaattctcca acgacgcttg
    25621 gattggtctt ttgtgcgaca ccaatcactc gtaagccatc ttcattaagt gactgtactt
    25681 ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa
    25741 taaatttaga aatgttcagc atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac
    25801 cagctggatc agcaaccaca acactcatac gacgacgttc aaaatcaaaa ggaatttcat
    25861 ccactttttg ataattgatc ttatctgtat ctaaagtttc tgttgcttca tcaatgatag
    25921 ccacatctaa caaatttttc agccctgttt gatagtagct atttagatac gcatggcgca
    25981 aaacacgatc atcttcattt ccttcacaat ccatatgata ttcaagaatg attttatctt
    26041 gtgtcaatgt ccccgtttta tccgtacata acacatcgat tgcaccaaag ttctggatgg
    26101 cattcaaatt tttgatcact gttccttttt tggccatcac acttgctcct ttgaccaaat
    26161 tcgtcgtaac gatcattgga agcatttcag gtgttagacc aactgctaca gatagtccaa
    26221 aaagaaaagc ttctagccaa tccccttttg taagcccatt gatgacgata actgctggag
    26281 ccatcaatga cataaatttg atcagtagca tggatgtttt atggatgcct aattcaaagc
    26341 ttgattgaat tggtttttca gatagcgttt ctgctacatg accaaatagt gtttcatttc
    26401 caacagaaac aatgattcct tccgcacttc cactgatgac attacttccc ataaagacaa
    26461 gattttcgta acttgtttcg ctttgctgat cgtcttcaca tactttcgca tgcttttcta
    26521 ctggatagct ttcccctgtc aatgctgctt gggaaatgaa taaatccttt gcggacaata
    26581 aacgcatatc cgctggtatc atatcccctg ctgaaagccg aacaagatct ccacagacga
    26641 tttcttccat aggaatctct gtgtactttc cattacgttt tacagctgcc gttactttca
    26701 caagtgattt cagtttttca gccgcttgat tggaacgtac ggactgaatc aacgtcattg
    26761 tcccgctgac gataaccata gtgaagataa tggctacacc gaaaagatct ttatctccag
    26821 gagctgctaa tacatattcc gtgatgaatg aaataattcc taatgcgatc aacactaatg
    26881 taaatggcgt gatgtatgct ttcaatattt ctacaatgaa tggtgttttt ttgccatatg
    26941 aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat ttgttcttct gtcaatccat
    27001 ttgctgatgc atggaatagt tcaaataatt cttgtttcgt ttttccagca aaagctgcat
    27061 agattttttg tgtatctttc tttttttcca tctcaatctc tcctttgtgt ccaaaagaca
    27121 gctgttctcg ctgcttttgg tctttagcta ttcatcggat cgatagagaa aaaactgaaa
    27181 ggggctgaga acagatagat aatagccctg gcctttcctc tagatccttg tttttcgaat
    27241 gactgtcatc catttgttct catctccttt actaagtaat tttaattcat gacttccaat
    27301 catttttcga atagttattt ttccaattag accacctcct ttttattttt caccgatggg
    27361 gatttgcggc aacaaaaaaa cctctcgcca aatactcagc gaaaggtttg aaaaagcata
    27421 ttaaaaaaag cttctcagat ctcatcgttg agttttgaca ctgtacaacg taaaaagcag
    27481 atacctgctt ttagctacct tatgaaaagc cttatctcga caattcctgt tctacccatt
    27541 ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta taggagcctc acctaacaaa
    27601 tcttattcgg ttttccttcg ttaatataac aactaaaaaa gaggatgtca aatcaatttt
    27661 ccatgttttt catacttttt ttaaaataca cacgttatat tcctgtctca cgcggtttta
    27721 tttgtctcat tttccacctc ccgctataat agaaatatag taggaaacga ggtgctgtta
    27781 tggaaagaac acttgttatt atcaagccag acggcgtgag acgccattta gtcggaagca
    27841 tcatccaacg ttttgaagca aagggactgg ccattgctga aatgaagttt gaaactatga
    27901 ctcctgaatt agcaaaagaa cattatcagc acttgactga gcgcagtttt tttgatgaac
    27961 tgatcgatta catgacttcc ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga
    28021 tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc cgatgctgtt ccaggtacga
    28081 tccgaggaga ctacgcgctg ccggggactg aaaacatcat tcacgcttct gattcccgtg
    28141 atgctgcggt aaaagagatc gcccgctttt tcccgccatt cgatataaac aaaccgtctg
    28201 aaaaagcggc gaagtgatac aaattctaaa ctgctggtct agttgctggg ttcagcaatg
    28261 gccagcagtt ttttctctaa ggacctccgt tgattctttc ctaacatata ttcgagcact
    28321 gtgaagaatt cttggtagtc tttcgtcttt tcttccccta aagcagcaga aatcaatctt
    28381 tgataataat ctgccatcaa tgtttcatag atcactgtac ttctttcttt atgtgctaag
    28441 ctccgtttca tatcttcatt cacactacgt gcctttttca accgaccatt ttctatataa
    28501 aacgaagtca ggttcattaa aaaagtaaag agatactgct catgattgtt caactgtgct
    28561 aatcgtcgca ttcttttttt gattttcaaa taattgttct caatgaaccg atcactgaat
    28621 atccaaggaa aatcagctag aatagatagt tcaaattgac catagtctgg tatctgcatc
    28681 aagtattctt caatgattcg gcattcataa cgtaaaaatt caaattctgt ctcagatact
    28741 tctgtttttt cgaccatgtg aagattcaaa accaaataat acagatcttc tgttttctta
    28801 aaatgcctac ggttattgct gatctcttta taaggatcaa agctgtcttc ttgtacccga
    28861 tctagcattc gtcttttttt ctttaacttt attggttcat ccagctggtt tcgcatccat
    28921 tcatattctg caagtgtcat gttcattcgc tctaaaaatg atctcaatgt tacaaatgac
    28981 atttttttca ctgaatttgt ctcaatcccg actagagaag tcttaaaaca gattccttcc
    29041 gctaattggg actgtgtcaa cttcttgttg actcgaagtt tcttgatcaa ttcaccatca
    29101 tacataatag ttctcctttt taatttaaat ttttccacat tcaataaata gatacgttaa
    29161 aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt cataaaaaat accaaccttc
    29221 aagcattaga atgttgtcta actactgaag gttggtttga ttgtttattg attatttttt
    29281 tgctgttcaa cgtctgttct tgttactgga tataagttgg ttgttgtttt attaccattt
    29341 tttgaaaaga cacttgtcga atgttcgcct ttttgctttt cgattgcttc attacgttcc
    29401 gcttcatttt tatagtcata ttttttcgga tcaatcgtct caaatccgtt tggcacataa
    29461 aaccgcaaga gattttcctg attcagctta tctgaaagac ttaacgcagt cttgactttt
    29521 tcctgatctt tggagatttc ctcatcgatt cctgctgctt tcgtatcgat gatttctcct
    29581 gtctgattat tgtaagcttt tcctcctagg acggtgtatt tgggtgtcac aaagttccca
    29641 tttcgaaaag caactacttg atcatgctga gaagataata agtctgtacc aaaatgaaga
    29701 taatctttat catcgattcc caatagatgt agtaatgtcg gcaaaacatc gatttctcca
    29761 ccgtattcat gattgactgt ccctttagta taaccaggca tatggatcat caatggcact
    29821 cgttgcatct gtgcgttgtc aaattcatcc catgtatctg gatctttacc aagagcgctt
    29881 gctaaatctt tattatctgt attcgagatc ccaaaatgat ccccgtaaat cacaaagatt
    29941 gaattttggt aaataccaga ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc
    30001 agataatgtg ccgttcgaac ataattatct actgtgctat tgccagtatt caaagatgga
    30061 aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt tggttacgga aagaaatttc
    30121 gtgtaaaacg gctgttgtag atgctcaaga tattgggcag attctctgaa taaatcttta
    30181 tctaatatcc cataacctaa cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa
    30241 ttgtcataac ctaagttttt atatacatga tctctattcc aaaaactagc tacattccca
    30301 tggaaaacag cgcttgtata gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag
    30361 acattgtctg acccaagttc tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt
    30421 tccaacatat tttcagcatc acttgtcttt ccctgtccta cttcatgaaa gaaattgtca
    30481 aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg
    30541 tctaccttca tattaatcaa aaactgctgg aaactttcta aatggagaac gatcacattt
    30601 ttccctttag cgatcccaaa tgtttcggga tttggctctg cataatgttt ctttgtataa
    30661 tctaaaactt catcaatccc attactgctt gcatgcgcgc gaacactgct agtcattcct
    30721 gttttgattc cgtcataaat agtatacgca tcgattccta gatatttgac aatatatgaa
    30781 cggtcaaatg ttcgctgcag caattgtgga cggttggctt cgctcagcgc tagatttcct
    30841 gaaaaaataa gccctgaaag acaggtaaca gcgatagcca tcggtttgcc gacagggttg
    30901 cttttgatag gtattttctt gaccttcaac caaaccagta gcccaatgaa cacagcgata
    30961 tccagccagt agatgacatc atggggcttc atcaaagaaa aggagcttcc tgacaatcct
    31021 tgcgacactt tcgaaaagcc taatacagat ttgactgtaa tgaaatctgt gaattcccga
    31081 taaaaaataa tattcaagta caacaaaagc gtatttaaaa tatcaatgat caaaagaata
    31141 gctaacgctg gctttggctt tttaatatac aaagaaaggc caaaaaacaa taaggtagtc
    31201 gcaattgggt tgatccacaa gataaagtac tggatcgtac cttccacacc taatgaaaaa
    31261 tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt
    31321 gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt atatcacttc tctctattca
    31381 cattttttct tattttgctc ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag
    31441 atttttttat tctttctaaa gattattccg tccacttttc acagcataaa accgctgcga
    31501 tgtaagcgat taaactaatg aagagataaa agtcttgtgg tgaccgcttc actccacggt
    31561 attcaaaagc cagtttctcg gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc
    31621 gcagctcctt atttcttgat gcaggacagc tttttcacaa cttcttcctt aaacggtgtt
    31681 ctcctgccaa ctcctcggcc acaagtcaca atatttataa aacatgagaa gctgtttttt
    31741 gaaattgttc cttgtggctc agagttaaac ggcaggctca agacctcttg atacacggtg
    31801 ttcaatcagc tgaccctcgg tattagatta tgtcaggacg ctaattttac aacctcttgg
    31861 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatataag
    31921 gagctatttt ttgaaattgc tccttgtggc tcagagttaa acggcaggct caagacctct
    31981 tgatacacgg tgttcaatca gctgaccttc ggtattagct caaaaaacgg caaaatatga
    32041 ggaactattt tgccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
    32101 tgtttttcat tgtccatagg attgatattg tccttgtgat gactcattcg taataattga
    32161 ggcttgattt tcttcacttc ccgttatcgt ttgtccactg ccagaatctg aaggacttgt
    32221 tcctgtcgac tcgttttttt gagaagagac agttgttttc ttgacttgtt catttgtctt
    32281 tttccattcc gctaaacgat cggcgaccac ttttttgtct actttttcag aataagcggt
    32341 gatcactttt ccatttttca aacgggctac tttgtcgcca tcatgagtca aaagcaaaac
    32401 ggtcaaatgc ttgtctttaa attgatattg tctgagagaa atcgcactaa gtacgtcatc
    32461 gatattaata aattgatttt cttcttttcc ttttggcaaa taaatgtcca attcgccttt
    32521 ttcgtaattg gcagttattt tatactccgc ccaccaataa gttgagaatt cttttttgaa
    32581 attatctact gattctttgc ttgctacgat tttttctgtt tctttctgag caagatctgc
    32641 tgtcattttt cctgaaaatg ctgcggataa aataagcagt aagaaaaaac cgaataacca
    32701 aaagagaaga aacggtcttt ttttcctcat gctttcctcc actcctattt aattttgact
    32761 attctattat atacaaaaaa tgaaatagaa agacaaaaaa gttgaaaaag atcgtaaata
    32821 attgaacttc tttttcacaa aatggtaaga atggagaatt tcacgcattt gctctcaact
    32881 attctttctt aagcctcaaa tccgttttta tctgatacgt ctttgaacca atgacccgat
    32941 tttttgattg ttctttttcc atcttgtgct aagtctactg aaataaagcc atatcgattc
    33001 ttatatgcat tggtccacga ccagttatcc atacatgtcc acatatgata gcctaaacaa
    33061 ttggcacctt cttgaatggc ttgatggaca tatttcagat gagattggac aaattcgatc
    33121 cgataatcat cgtggatcat gccatcttct ttgataaatc gtgcttcgtt ttctacaccc
    33181 ataccgtttt cagaaataaa gcagtcgata tttccataat gatctcgtac attgatcaaa
    33241 atatcataga ttccttgttc atagatctcc catccgcgat aaggattcat tttacgacca
    33301 ggcatttcat aattgtcgaa ataatcctca ggcattggac cgtttttagt atcgattggt
    33361 gtttcttttg cttttacccg gcgtggctgg taataattga tacctaaaag atctactgta
    33421 ttttctttga taaccgtcaa atcctctgca tggatcacag gcatgagatc aagttctttt
    33481 actatctcaa tcaattcact tggaaactcg ccttttacag ctggatcaag gaatgaacga
    33541 ttgaagaacg catctgctat ttgcgctgct ttaacatctt cagggtttga ttcgtctctt
    33601 ggatagcttg gtgtcagatt caagatgata ccaataacac caccttgctc catttcatgg
    33661 taagatttga tggctaacgc tgaagccaac gcttcatgat agccaacttg gatcgctttt
    33721 ttcatatctg actcttctgg ataatgccat cgatataaat aacctgcttc gactggaacg
    33781 attggttcat tatgagtaaa ccattttttt actttcgaac caaacaattc aaaacaactt
    33841 ttggcatatt tttcataggc ttcaaccgtt tctcggttca accatccgcc tttttcttgc
    33901 agatacatcg gcatatcgaa atgatacaga ttcacaaagg gttcgatacc gttttcaatc
    33961 aactcatcaa aataacgatg atagaaacga acggcttctt cgttgattgt tccagattca
    34021 tcaggtatca aacggctcca ttggatagat gttcgaaatg aattatgccc agtctgtttc
    34081 atcaactgta catcctcttg atacttcgta taaacttgcg aggttttttc aggtcctact
    34141 tgatcaaaga acttttgcgg ttcttttttg taccaataat caaaaatgtt ctctcctttt
    34201 ccatcgcctg aaatccttcc ttctgtttgt gggccacttg ctgcagaacc ccaccaaaaa
    34261 tttgctggaa attgatattt tttcatcaaa gcacctcttt ttctttttta gttcgtttta
    34321 gttgattcca tgtatgatat tttccatcac aatcactttt tcattttctt cctcttcttt
    34381 tttgattcgt tttaacagct gttctgctgc tcgttccccg attttatctg ttggtggcgt
    34441 gatcgtgatt ttcggaagga tactcagatc gtttttatct aaataatcat agtgactgaa
    34501 cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa cattcgatgg caccaatcga
    34561 catattgtaa ttgctgacaa aaatagcagt tgcccctttt tctaataatg cttttgcccc
    34621 gtaatagccg ctttttctag aatagtctcc tgtaaagacc tctaattgga tcgatggatc
    34681 attttggacc gcttctttgg ctcctgctaa acgttctctt gctacgtagt ctgtttgagt
    34741 ggcagcaata aatccgattt ttttatgtcc ttgttcacca tgatgagcga tcacttttgc
    34801 tacactttct cgatcattca ctaatattgt atcaatacct gctggtcctt ctggacaatt
    34861 caatgcgata actggaatac tcatttttgt gagcatttca aatccagacc actcttcttc
    34921 ggataagaag acaatcagcc catcgatcac atgcttcatc aggtattcaa ttttttgttc
    34981 aattttttct ggctgatcgg caaacccgct taataataag ctatacccat tttcttcagc
    35041 tactctttcg atccctgaga caacttccga cccaaaacgg ctggaaatcc cgtttatcaa
    35101 aagtccaata gagaagcttc gattattctt caacccttta gcaatgatgt tttctttata
    35161 gtccaacgct gaaatcgctt ctgctatttt tttcatgttc ttttccttca gttgctgacc
    35221 attcaagtaa cgggaaactg ttcccactga aacaccagcc atcttagcca catcacggat
    35281 cgatgccata cattctcact cctgccagtt acatttgatt tctctttctt gttgttcgat
    35341 tatgatgacc gcattctttg catagtgttg cttgtcatca atatccatat aactaaagtc
    35401 ttcagtagag caacttcctc tcactattaa ttatacttaa acaagtattt gaaccggttc
    35461 atttttaata tagcattttt ttaaaagatt gcaacacttt tttaaaacgc tttctgaaag
    35521 tggcggattt tttgcctttt taaaatttta ttctaaaatt tactctattt gtgggtgttt
    35581 tgtgcgtttt tctcttccat ccgtttgaag ttttacattc caatggtata atttctttat
    35641 cctatcaaat gggggaagta ttttgaagaa attaatcgta ctatttattt tgtctgcggg
    35701 acttttgaca ttgactggct gtgatttgtt tggcggctct gaccaatcct cggacgaaac
    35761 aaccgattct tctgtcttaa aagcggaaaa agcagatacc accaacagtg tatatacgct
    35821 aaaagtacgt aaagatatgg atttatccgc gggacagttg acatttacag ataaaaaaat
    35881 ggaatggaca cgcacgtatc agacacaagg aactgattct tctgaatcat ctgaagaaaa
    35941 aacagtgctg acagatatca agatcactac caaagatgac gaatatatta tttcaggaaa
    36001 agaaaataaa aaagaaacaa cagtcatatt tacaaaaatc ggtaattacc gtttgaagga
    36061 tgaggatggc aacatctaca gtctatagtg ccgtctgttg gatcttcttt tgtttctccc
    36121 cttcttttct gctatagtaa agaagcaaga tgattgtaaa ggagacgaaa tggatgatcg
    36181 atagtcctga ttattgtaca tgtaataaca acaaaaatat ccatacagaa tttaatgacc
    36241 aaggaagctt gagcatatgc gacaactgtc acaaactagt caaagattca tttaaaacat
    36301 ttgaatctta tcctgttcaa aattatgtaa aaagtagaag aagataaaca aatccaagca
    36361 aaactcacta tggctgattt ttatgaactt caaactcagc ttcttccttt gatcaagctt
    36421 catcagtaaa acaaacagtg gatcatttca gtttacaaat caaagagtaa agatttattt
    36481 atttcaaata tttgaatata aaataaccaa acgatagaaa ggcttattct acatcacgta
    36541 gtaatttttc tttcccgtcg tttggtttt
//
LOCUS       DAZKCZ010000015        36422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000015 DAZKCZ010000000
VERSION     DAZKCZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.015"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..632)
                     /locus_tag="ACG8KN_000642"
     CDS             complement(51..632)
                     /locus_tag="ACG8KN_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFP9187725.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(638..1897)
                     /locus_tag="ACG8KN_000643"
     CDS             complement(638..1897)
                     /locus_tag="ACG8KN_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9187726.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIAPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1899..2945)
                     /locus_tag="ACG8KN_000644"
     CDS             complement(1899..2945)
                     /locus_tag="ACG8KN_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187727.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQRPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3180..3455)
                     /locus_tag="ACG8KN_000645"
     CDS             complement(3180..3455)
                     /locus_tag="ACG8KN_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFP9187728.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACG8KN_000646"
     CDS             complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACG8KN_000646"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9187729.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACG8KN_000647"
     CDS             complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACG8KN_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9187730.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACG8KN_000648"
     CDS             complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACG8KN_000648"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9187731.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAIQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7283..7918)
                     /locus_tag="ACG8KN_000649"
     CDS             complement(7283..7918)
                     /locus_tag="ACG8KN_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFP9187732.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7984..9387)
                     /locus_tag="ACG8KN_000650"
     CDS             complement(7984..9387)
                     /locus_tag="ACG8KN_000650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9187733.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGFLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVIHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKHQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(9374..10405)
                     /locus_tag="ACG8KN_000651"
     CDS             complement(9374..10405)
                     /locus_tag="ACG8KN_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9187734.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYERILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10436..10657
                     /locus_tag="ACG8KN_000652"
     CDS             10436..10657
                     /locus_tag="ACG8KN_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFP9187735.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10822..11559)
                     /locus_tag="ACG8KN_000653"
     CDS             complement(10822..11559)
                     /locus_tag="ACG8KN_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9187736.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGNSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11713..13053)
                     /locus_tag="ACG8KN_000654"
     CDS             complement(11713..13053)
                     /locus_tag="ACG8KN_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9187737.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(13290..14813)
                     /locus_tag="ACG8KN_000655"
     CDS             complement(13290..14813)
                     /locus_tag="ACG8KN_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP9187738.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(15191..15802)
                     /locus_tag="ACG8KN_000656"
     CDS             complement(15191..15802)
                     /locus_tag="ACG8KN_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9187739.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(15925..16049)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(16131..16847)
                     /locus_tag="ACG8KN_000657"
     CDS             complement(16131..16847)
                     /locus_tag="ACG8KN_000657"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9187740.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(16853..17422)
                     /gene="scpB"
                     /locus_tag="ACG8KN_000658"
     CDS             complement(16853..17422)
                     /gene="scpB"
                     /locus_tag="ACG8KN_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFP9187741.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISFEEDVSPDLFFDGMNKGE"
     gene            complement(17406..18176)
                     /locus_tag="ACG8KN_000659"
     CDS             complement(17406..18176)
                     /locus_tag="ACG8KN_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFP9187742.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(18173..18544)
                     /locus_tag="ACG8KN_000660"
     CDS             complement(18173..18544)
                     /locus_tag="ACG8KN_000660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9187743.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGVYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(18563..19450)
                     /gene="xerD"
                     /locus_tag="ACG8KN_000661"
     CDS             complement(18563..19450)
                     /gene="xerD"
                     /locus_tag="ACG8KN_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9187744.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(19546..20019)
                     /locus_tag="ACG8KN_000662"
     CDS             complement(19546..20019)
                     /locus_tag="ACG8KN_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9187745.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(20124..20987)
                     /locus_tag="ACG8KN_000663"
     CDS             complement(20124..20987)
                     /locus_tag="ACG8KN_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFP9187746.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(21183..23324)
                     /locus_tag="ACG8KN_000664"
     CDS             complement(21183..23324)
                     /locus_tag="ACG8KN_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFP9187747.1"
                     /translation="MMKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDA
                     AMQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIE
                     KNEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHRED
                     TKEEHAKELLKMISEKIAQENKVEPEHEAEKDTEKDISGVNAAGKESLKTNDLKTNDL
                     KTDIPEKTETLSEKQSEDEKSNGLPEADKKEMLEKADKKATESDGINNEADAETQSAS
                     EDSTALEAKIEKEENQPELIEAETEPITIVPNTEKTPQESNPEKKDLDKTQKKKSKKV
                     PYLALAAVLLLGGGGWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFI
                     RADMIHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDH
                     LADDIKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVV
                     DSATRDQYKTAKEAVEKVKNTDLSAQLNEQLQQVDKMLTDKEKKEEEEKKKAEEAKKA
                     EKAKKAAEEQAKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARAS
                     DIADASNPAWTWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKS
                     ALMKGIGESALPFYIVTINCKTGWFGGNGSN"
     gene            complement(23541..24650)
                     /gene="rpoD"
                     /locus_tag="ACG8KN_000665"
     CDS             complement(23541..24650)
                     /gene="rpoD"
                     /locus_tag="ACG8KN_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9187748.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKQQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(24674..26515)
                     /gene="dnaG"
                     /locus_tag="ACG8KN_000666"
     CDS             complement(24674..26515)
                     /gene="dnaG"
                     /locus_tag="ACG8KN_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9187749.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(26697..29012)
                     /locus_tag="ACG8KN_000667"
     CDS             complement(26697..29012)
                     /locus_tag="ACG8KN_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9187750.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(29197..30057)
                     /locus_tag="ACG8KN_000668"
     CDS             complement(29197..30057)
                     /locus_tag="ACG8KN_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFP9187751.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(30116..30748)
                     /locus_tag="ACG8KN_000669"
     CDS             complement(30116..30748)
                     /locus_tag="ACG8KN_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFP9187752.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            30915..31676
                     /locus_tag="ACG8KN_000670"
     CDS             30915..31676
                     /locus_tag="ACG8KN_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9187753.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            31702..34293
                     /gene="mprF"
                     /locus_tag="ACG8KN_000671"
     CDS             31702..34293
                     /gene="mprF"
                     /locus_tag="ACG8KN_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9187754.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(34481..35668)
                     /gene="tuf"
                     /locus_tag="ACG8KN_000672"
     CDS             complement(34481..35668)
                     /gene="tuf"
                     /locus_tag="ACG8KN_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9187755.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            36036..36392
                     /locus_tag="ACG8KN_000673"
     CDS             36036..36392
                     /locus_tag="ACG8KN_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9187756.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        10119 a         7707 c         6251 g        12345 t
ORIGIN      
        1 attccacatc cagctgatct ttgtcttttt tagtactata ctatttttaa ctaattgtta
       61 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
      121 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
      181 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
      241 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
      301 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
      361 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
      421 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
      481 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
      541 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
      601 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
      661 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
      721 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
      781 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
      841 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
      901 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattgctta
      961 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     1021 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     1081 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     1141 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     1201 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     1261 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggtg
     1321 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     1381 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     1441 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     1501 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     1561 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     1621 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     1681 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     1741 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     1801 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     1861 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     1921 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg acgttggtat
     1981 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     2041 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     2101 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     2161 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     2221 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     2281 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     2341 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     2401 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacttg ccgctcgctg
     2461 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagtac tacacgattt
     2521 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     2581 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     2641 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     2701 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     2761 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     2821 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctgg
     2881 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     2941 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     3001 agataagagc aaagctcacc tcataacttg gaaataaata aaaattaaat aaaatacatg
     3061 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     3121 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     3181 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     3241 atttcgattt cttcaccagt ttgtgggttg cgacctttgc gagctgcgcg ttcgcgaact
     3301 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     3361 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     3421 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     3481 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     3541 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     3601 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     3661 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     3721 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     3781 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     3841 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     3901 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     3961 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     4021 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     4081 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     4141 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     4201 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     4261 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     4321 tatcgatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     4381 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     4441 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     4501 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     4561 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     4621 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     4681 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     4741 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     4801 ccataaaggg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     4861 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     4921 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     4981 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     5041 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     5101 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     5161 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     5221 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     5281 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     5341 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     5401 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     5461 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     5521 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     5581 gaccaaggtc caggctgtgt gtctttgatg gacaaggaaa cacgttcttg ttcaggattg
     5641 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     5701 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     5761 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     5821 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     5881 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     5941 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     6001 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatc
     6061 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     6121 cgttttgaaa gtaggtagct gccgttttct ttatctttgc cgatcgtact gataacgact
     6181 aagtctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     6241 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     6301 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     6361 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     6421 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     6481 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     6541 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
     6601 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
     6661 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
     6721 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
     6781 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
     6841 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggatagcaa
     6901 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
     6961 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
     7021 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
     7081 aggatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
     7141 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
     7201 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcaaaaaaa
     7261 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
     7321 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
     7381 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
     7441 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
     7501 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
     7561 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
     7621 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
     7681 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
     7741 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
     7801 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
     7861 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
     7921 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
     7981 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
     8041 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
     8101 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
     8161 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
     8221 ttcctgatgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
     8281 catccatttt ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag
     8341 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
     8401 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
     8461 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggatcacat aacggatatc
     8521 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
     8581 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
     8641 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
     8701 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
     8761 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
     8821 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
     8881 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
     8941 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
     9001 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
     9061 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
     9121 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
     9181 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaaaaccc
     9241 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
     9301 tccatcaagc aacgcctgaa tgatttcttc ttggccggga cgaaatgaat caaatccaaa
     9361 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
     9421 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
     9481 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
     9541 gctcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
     9601 ctctttttaa atgacggatt gccataatct ggtcaattgt ctgaccattc ataaacatct
     9661 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
     9721 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
     9781 gttgcagata aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg
     9841 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
     9901 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
     9961 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    10021 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    10081 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    10141 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    10201 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    10261 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    10321 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    10381 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    10441 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    10501 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    10561 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    10621 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    10681 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    10741 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    10801 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    10861 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    10921 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    10981 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    11041 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    11101 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    11161 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    11221 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    11281 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    11341 gagagcacca ggataaaaaa taagtaatgg agaatttcca tgattctttt tctctccttc
    11401 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    11461 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    11521 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    11581 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaaataa gagagaaatg
    11641 caaactttcc agcatcaaat aaaaaaagac ggaatatcca tttgaatatc cgcccttttt
    11701 gtatgtttat ttttatttag cacataaacg atacacagct tctgcataaa tttccattgc
    11761 tttatacata ctacttaatt cccatcgttc attcgcctgg tgcataaagt caggagtatc
    11821 agggaacatc gcaccgaatg ccacacactg gttcatcgta cgagcaaaag tagcgccgcc
    11881 tgatacaaac ggttcagtca tgtctccagt aatatcgcga tatgtcccca atagattttt
    11941 cactaactca ctgtctagtg gcacgtataa tgaatcaagg aagtcaaatt cttcataagt
    12001 caacccatat ttttctgctg ttttagttaa tttagctact aaatcatctt tcttgaatgt
    12061 aacagggatt ctcatatcta caccgatttt tgtttgttct ggtgtgattt ccaaacttgc
    12121 aaagttcatc gttagctcgc cggattgttc atcttctgtt ttgcccacga cagcttcacc
    12181 agttgcattt tcttgaacta attttcctaa gaaattgatt gggccaaaat cgaatacttc
    12241 tgaaagagca attgccagac gagaaatagc attcactcct tgtgctgcat ctttcgcgtg
    12301 gatactttta ccaagaacga cgatgccttc accttgttct tcgaaatcga attcgtgttt
    12361 tttcaacgct tcttttactt cagaaagctt ttctccagag taaggagctg catcaggaac
    12421 aacattcaac gctcctccag cttttacaga aaactcgttc gttcctggtc ctgtcaagta
    12481 agcttgcaat aagccttttt ctgcataaat taaaggaaat tcagcatctg gcgcaaatcc
    12541 ttgcgtaatt ccttcttctt tttcgttata tttttctaaa caacgccata aattttcttc
    12601 atctgtacca aagataaaac gaatacgtgt cttgaattcc acgccagcat caatcaaagc
    12661 tttgacggca tacattgccg caatagaagg acctttgtca tcttgggatc cgcgaccaac
    12721 taaccagccg tcttttatag ttggatcgaa tggttttgtt tcccaattat tttcgtcccc
    12781 tgctggaaca acgtccatat gacacaagat accgaataat tcatctccag agccaatctc
    12841 tgaataaccg taatagcctt ctggatcttt aaatgttcta aatcctagct tttcactgat
    12901 ttccaatact ttatccaaag cttcaatcac ttttttaccg aagggatgtc cttgtcctga
    12961 atctgcttca tctagtacgg aaggaattcg gatcaattcg ctcaatgatt ctaatgcttg
    13021 ttcatgatgt tgttctgtca caaatttttt catgatatta tcctcctaat atgttaaaaa
    13081 gggtcatgtc aaattcctcc gacacaacct cttcttctca aataatgacg ttttaagcct
    13141 gctccatcag caagcagaat gttcttaaca aatgatgaaa gaaaaaacac tcactgaatg
    13201 gagctattga cttgtccacg tgcttatcga tagccgttta tcggtttctt cattcttatg
    13261 atagaccttg tacctgtatt tgtctaggtt tagaacattg ctgctacacc taggatcaca
    13321 cagatggcta caaataatat cgcaattaat ttaccggtaa atttccacca cactgaaaca
    13381 tcgaaacgag caattgctac agcacccatt acgatagcag atgttggtgt aattaagttt
    13441 actaaaccag atgcagattg ataagcagtg attactaaat ccttaccgac gccggcaaat
    13501 tcacccatag gtcccatgat tcccattgtt gcagcagcta agccggaagt tgaaggaatc
    13561 aagaatgaca tagggatata gaaaataaat gtaataatga tgaatacact tgaagataat
    13621 gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg tgattaatcc attgttcata
    13681 accacttgga taccacgagc aacagctact acgattgcta caccgatcaa atcagaagca
    13741 ccagctaaaa attcagagac aaattcagac tctttcatgc cataagtgat tgctactacg
    13801 atggacatta ctaagaataa catagtaatt tctgggaagt accagtcacc taatggaagc
    13861 atgcttttac ctagaacagt acctagtacc ggaaggtttg ttaaccagtt tgtcaatgat
    13921 tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa tcaaactgat gatcatgata
    13981 ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt ttttcgtgat tacttcttga
    14041 cgaccgattg cttcaatatc gaaatgcttc atatcttctt tacgttggtt gtatactaat
    14101 gattttgttg gatctttttc gatctttgat gcataacgat acacatatac aatagaaata
    14161 gctaacaaaa taacgaacat caagaatcga agaccaatac cttcacccat cgaaatccca
    14221 acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg ttgaagctag acagcctact
    14281 tgagacccta ctaatacgat tgctactgct actactgtat caaagcctac agccatcata
    14341 actgggatca gtaaagcata gaatggaatc gtttcttctg ccattccgta agtagagccg
    14401 cctaatgcaa ataacagcat caatactgga ataagcattt tttcttttcc tttgaatttc
    14461 ttcacaactg aagcaatccc ggcatctagt gcccctgttt tgttgataat acctaaaaat
    14521 ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag caggtgtttc tttagttagc
    14581 tcccccgctg gtacacctaa catcccattg attggcgcca tgaaaatatc ccacagccct
    14641 tgaggatttt gttgtactcg ttcatatgat ccagcgacga tgttgcctgc atcgtctaca
    14701 ctatagtgcc cagctggtat aaaccaagta agtacagcga ttactgcaat aataataaat
    14761 aagaccgtat acgctgaagg catttgaaat tttttctttt ttgtttgttc catcttcttc
    14821 ttcctttctt ttccgttaga gacaacaatt ttggctctaa tgttatttta tcaaaaagag
    14881 tatcagaaca aaaataataa cacgcatttt gtaaaacttt tgttcaaaaa gttcgttttt
    14941 ccctattccc tcctcttttt tcaggttttt aaagctttct tctttttcat tgtagcgttt
    15001 cctttttttt ataaacaaac attttcgtct tttaaaagga gaagaatttc acatttgcaa
    15061 agaattgatt gatttttatg cataaatcaa aaaaatactc aaaacaaaca aataaaacgt
    15121 ttacattatt gcatgtcttg tatttttttc caaaaaaaaa gaccaagaaa tttcttgatc
    15181 tttttgataa ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta
    15241 accatggaag aagttgcgca tgcagcacta aaaagacagc gctcacgatg aatcctttga
    15301 tcaagttgaa aggaacgatc ccaatcagta catagtttgc taaagacatg cctagcatct
    15361 gtgttgcgtt cagtcctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga
    15421 tactcataaa aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc
    15481 ctttatgctg tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg
    15541 ataagaaact agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat
    15601 gtaatactga acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc
    15661 tgatcaatac cgggatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg
    15721 gaaaagcaaa atactgtaaa actaacgcga tcgcagcaaa catcgctact gcaaccattt
    15781 tctgtacacg ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt
    15841 tttgacgttt ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta
    15901 tttgatcaag cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc
    15961 ggaatttcac cgattcagct attttttgca aaataggtcg tggactataa ccaccggtcg
    16021 ggaatttcac cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat
    16081 acatatctat tcaaaaaacg caacatctaa cacttataaa aagtaagtgt tcattttgag
    16141 tggttcagca aactgctgac ttcttttttg ttcagttcac ggtaatctcc aggacgtagg
    16201 ccttgtaaag tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact
    16261 gcttgaagca tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata
    16321 ctgtggtttg tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt
    16381 ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt
    16441 acctttgcaa cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa
    16501 tctccatcat ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga
    16561 tagatacgtt cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct
    16621 gcaacagcag aaataactcc tttaggtttg tacaataaat aataaccgta ttcttcttga
    16681 taaattggga cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc
    16741 cttacaactt gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga
    16801 cgagaagtga tgccagcgtg ggcaattact ttctgcaatc tttccattac aattattctc
    16861 ctttattcat tccatcaaag aatagatctg gtgacacgtc ttcttcaaat gaaatttcca
    16921 tttcttgtgt atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc
    16981 cagttgttcc atataatatc ggccgacctg gcccttctac tctacctttt tcttcaatca
    17041 gtttatgggc agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga
    17101 tttcagctct tgtgattggc tgtttataag cgatgattgc taaggtttcg acagcggctt
    17161 gagataatgt gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt
    17221 tagtcgttaa tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt
    17281 gagtgtaaga ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta
    17341 acagtccgct taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt
    17401 caagttcact taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat
    17461 tcatagtttt ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc
    17521 ataaaagtag tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag
    17581 ccatgcgcat ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg
    17641 tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc
    17701 tgcatggcac ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca
    17761 tccgctaacc catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct
    17821 ttttctgtca gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc
    17881 aattcttctc gaggatcttc ttcccataat tcttcatcat ctgtgacgaa ttcttggaca
    17941 ggaagcaatg tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct
    18001 gcaacagcta gctctagtgt tttcattgta tggatataat tcatatattg ctccgtgatc
    18061 gtagcgatcg gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga
    18121 tcgagaggac cttcgaatac atctaattta atattgatct ccgtcaactc attcactctt
    18181 tcttcttttt ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga
    18241 tggagacatc cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac
    18301 cgcgataaga cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt
    18361 aaacaccaat aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat
    18421 aatcgtcttc agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt
    18481 tcttatggaa actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta
    18541 acatcttctc accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca
    18601 ttcttttctt cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca
    18661 attcctgtac tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc
    18721 gcagcgtatg tggtgttact tctttgtaaa tcccagcctc tcgtactagc tgcttcagat
    18781 ttttccagat tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt
    18841 cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga
    18901 tcgcataatc acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca
    18961 gtccaagaga aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc
    19021 ctgtagcata catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat
    19081 caggtgcttc aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg
    19141 ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta
    19201 aaaactgatg gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt
    19261 tttcttcatg caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg
    19321 aagcaatttt ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt
    19381 gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt
    19441 gctctttcat aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga
    19501 tagtcttttt ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt
    19561 tcttttttct ttttcatagc attcctgaca gattccatgg aacgttagcc gatgatcttt
    19621 tactaaaaaa tgataacgtt cttctactac ttgctccact tcaagtaaaa gatcctcttc
    19681 tacttcctcg atcgtgccgc agttcaagca tagtaaatga tgatggaaat gtttggcgcc
    19741 ttcttttctc aaatcataac gggctaatcc atcattaaaa cttactttat cgacaatttt
    19801 caattctgtc aagatttcaa gggttcgata aacagtagca agtccaattt ccggactctt
    19861 ttttttcact aaaaagaaga tttcttctgc agataaatgc tctttctcat tttccaaaag
    19921 cacaagcaga gtagcttctc tttgcggtgt cagcttaaac cccgcttcat gtagctgctg
    19981 ttttgttttt ttcatcgccg aaatagcatt catgaccatt tactcaaccc caacaattta
    20041 taataattac aaataaaaaa agatataaca ctatgttaat taaaattatt ataagctagt
    20101 taagaaaaac atgcaaggga ttctcaatta gattttttga ctagttttgt cacaccgttt
    20161 tcttgtgtaa tcaatccttg tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg
    20221 ctgataccaa aagctttcat gatttcttct ggatccgatt tatctgtaaa gttgatttgc
    20281 tgatctgcag aacgttctaa aatggttaaa atcatttgtg catcatctga tatagcttca
    20341 tatgctcttg gcttcaatga aagattcagt acaccgtctg gtcggacacc aatcactcga
    20401 ccttttattt gctcgcctag acgaggttca ttatatcgtt cagaaggatg gataaaccca
    20461 agatagaaat cttccgttaa aacgtacgta cctgttaatt tcaagcggta tgctgttcct
    20521 atcacatctt tgtttttcat ttcttctgtt ccatatttgc ttaaagattt gaatattttt
    20581 tcatctgcta aactgcccca aatacggtct tttgcatcaa ctctaaggga aatcatcaag
    20641 cgatcccctt ttttaggcca tagctctgtc attgtcggca attcatcaag agatacgaca
    20701 aagtctttat ctggaagacc gatatcgaca aacgcaccta aatcttttcg tgtagcagta
    20761 acgattccaa aagcgtaatg accgatacgg cttttaggaa tttgtgtagt gatccgattt
    20821 tcatgttttt gattttggta gccgaatcct tccactgctt caccgatttt gtgttcacct
    20881 tctgatttgc ttaagccaaa ggtttgtccg tttttttgga caaagtagtt tttttcattt
    20941 tcatcaataa taatacctgt aaatatagtt gctaataaat cgttcatttt tttctccctc
    21001 tatagtgaaa ggttttctta atacatttct ttgctatcat aacataatgt tatctacttg
    21061 aatagctgag ccttggtttg gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac
    21121 tccccttttt cgaataaacg gtgttaaacg gcaggctcac agcctctttc atttgcctaa
    21181 aattaattgc tcccatttcc tccgaaccat ccagttttac agttgatggt tacgatgtaa
    21241 aatggcaaag cactttctcc aatgcctttc atcaatgcag attttgttga agtggcatat
    21301 agattgtaat agccttcccc attttcgatt ctggcttttt ctaatctgta tcctccttca
    21361 acgatatatc cacgttggat acatgtagca atcactttgc tttggacacc tggtgcccac
    21421 gtccatgctg gattagatgc atcggcaata tcacttgctc tggcagattt gattggctga
    21481 ttcgctgagt ttgaagagga aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg
    21541 accgcagctt gttgagcggc ttgagctgca gctgctgctt gagcggcttg ttttgcttgt
    21601 tcttcagctg cttttttcgc tttttctgct tttttcgctt cttctgcttt tttcttttcc
    21661 tcttcttctt ttttctcttt atcagtgagc atcttatcta cttgttgcaa ttgttcattc
    21721 aactgagcag atagatctgt atttttcact ttttctactg cttcttttgc cgtcttgtat
    21781 tggtcgcgtg ttgctgaatc cactactttt ccgtctttaa agactatagc tgttttttct
    21841 ttagctgttt gcaattcagc atattgattt ttagcttctg tttgtgcttg ttgaatcaat
    21901 tttccaaaat ctgtgttatc cgtttcgatt gcttggatct ctttatctgc tttcaacttg
    21961 atgtcatctg ctagatgatc atcgacgatg gcaggtgcta tgaaccactc attcactttt
    22021 tggatatctc ggatcttaga ttggatatct tggtaagttg tttctaactc tgaatatcct
    22081 ttttcattct taagactgga taaatcattt tttagttttg ataaatcttg atgaatcata
    22141 tctgcacgaa taaattgatg gtgatcatct gtataaaagg cagcaagttt ctcatgaagt
    22201 tcttccattt gttgctgtct ttttgcttct gctgctactt cggcttctac tttttgatga
    22261 tggtcataat aagcccagcc tcccccacct agtagaagaa ctgctgctaa agcgagatac
    22321 ggcacttttt ttgatttttt cttttgtgtc ttatctaaat cttttttttc tgggtttgat
    22381 tcttgaggcg ttttttcagt atttggcacg attgttattg gttctgtttc tgcttcaatc
    22441 agctcaggct ggttctcttc tttttctatt ttagcttcaa gagcagtgct atcttcactt
    22501 gcagattgag tttcagcatc ggcttcatta tttattccat ccgactctgt tgccttcttg
    22561 tctgcttttt cgagcatttc ttttttatct gcttccggca acccattcga tttctcgtct
    22621 tctgattgtt tttcagacaa tgtttcagtt ttttccggga tatccgtttt tagatcattc
    22681 gtttttagat cattcgtttt taatgattct ttacctgcag cgtttactcc actaatgtct
    22741 ttttcagtgt ctttttcagc ttcatgttct ggctctactt tgttttcctg tgcaattttt
    22801 tcactgatca ttttgagcag ctctttcgcg tgctcttctt ttgtatcttc acgatgtgct
    22861 ttgatataag cggatagtat cgaattttct tcctcattat cgacgatttc tttcagttca
    22921 gctactcttc ttttttgttt tgattcaagc gtttcttctt gattctttcc tgattcttcc
    22981 gaagtctcat gaacagttgc tttcttctgt tcatttttct ctatattagt tacttgatcg
    23041 tgagcaggtg gttcttcgtc ttctggttct gctttttcga ataattccat tacagattct
    23101 agaggtaaat ctttcaattc cgaccattcg attggatcgt ttaattctgt atcaggaaag
    23161 actgcatctt cagttgattt atcctgcata gctgcatctg tatttgtttc ttcaattgat
    23221 tcttttattt gctgatcttc tgcagttatt tgatgaccgc agtctggaca gatagtattc
    23281 tgctccattt ttttcgatcc gcaatttgga cattttttca tcacttagcg atcggctcct
    23341 ttactaggtt tacgctacca tttaatctat catattctat acgtcacgcc aatagcttat
    23401 ttttaggatt tacttaaaaa taatgaatga atttccttcc tatagaaaaa aaagcttgag
    23461 actgcgtagt ctcaagcttt ttttctttta aaataaacca aacagtttta ctgatttctc
    23521 ttctggtcaa tagttcatgc ttattctaag aaatctttta attgttttga acgagaagga
    23581 tggcgcaatt ttcttaatgc tttagcctcg atctgacgaa tacgttcccg agtaacacca
    23641 aatactttcc ctacttcttc caatgttctc gtccggccat catctaaacc aaaacgtaag
    23701 cgaaggacat tttcctcccg gtcagtcaat gtatctagta catcttccaa ttgttctttt
    23761 agtaactcat aagctgcgtg ttctgctgga cttgttgctt cctgatcttc tatgaaatca
    23821 cctaagtgag agtcatcttc ttcaccgatt ggcgtttcaa gtgaaacagg ttcttgcgcg
    23881 attttcaaga tttctcggac tttttctgtt ggaagatcca tttccgcacc gatttcttct
    23941 ggcgtaggtt ctcttccaag gtcttgcagc aattgacgtt ggatacgtat caatttatta
    24001 attgtttcaa ccatatgaac tggaatacgg atcgtacgtg cttggtcagc gattgctcga
    24061 gtgatggctt gacgaatcca ccatgtagca taggttgaaa acttaaatcc tttgcggtag
    24121 tcaaattttt caactgcctt catcagtccc atgtttcctt cttggatcaa gtcaaggaac
    24181 tgcatgcctc gtcctacata acgtttagcg atacttacta ctaaacgaag gtttgcttct
    24241 gccagacgct gtttcgcttc ttggtcgcct tcttcgattt tcaatgctaa cgctacttct
    24301 tcttccgcag ttaaaaggga gacacggcca atttctttca gatacatacg aaccgggtca
    24361 ttgattttta caccggttgg cgcagataga tcttctgttt ttgcttgttc agcttttttc
    24421 tcagcactct tcaagctgtg aattgatgga tcaccatttt catccaccac actgatacct
    24481 gcgtcttcaa tcttttggat caatttgtcc atttcatctg cattcaaagt gaatggtgta
    24541 gcaagctgat tggataaatc atcatataca acttgttgtt tgggtttgtt ttctttgatg
    24601 aaagctgcta ctgctgcttc atacgttttt ttatctgttc catttgccat aaaagaaggc
    24661 cccccttgag tagctatttg gcttgtttta actgtttcgt taaattgatg atttcaatcg
    24721 tcaattcaag ctcaagctgt ttgtttccag ttcgtctggc ttcttgctgc atttgttttt
    24781 ttaatgttat ttcatcagct aagccagatt tgctgatcac atctagcaag tcttggattt
    24841 ctcgtggcga actttcttct gccaccggta aatattcgat ttccaccacc aaccgcctta
    24901 aattttcatc ttgcaaaaag tctaaaaact ctgcaaggtt gaattcataa tgttgtgaga
    24961 catatgcatc gaataaaaaa taaacttctg catatgcatc atgaataaac tgaaattctc
    25021 gttcttttaa aatattacgg acagaaactt ctttaaatgc acgatataat agaatctgct
    25081 ctgccttttc tacttgcgtc aaaggtctct ttttccgtgc agtttcttgt ctcgtaacct
    25141 gagtgtgttg actttcctga ggttcttgac gattttgctg atttgtcctt cgaagatcgc
    25201 gcagttgttt ttgtatcgtc tctctgccga tctgaaactc tgttgacagt tgagatagat
    25261 acatatcttg ttcgattggt gaaacaactc ggctaagttc aactagtaat tcttcgaggt
    25321 attcgatctt gtctttttca ttatctaaat ttttcccttg ttttaaataa cgtgatttaa
    25381 aagtaaagac tgtttcgcgc ccatgagcca ctaattcttg gaaagatgct tctccatact
    25441 tacggagata ttcatcagga tcaagctttt ctggaacgac tacgacagaa agtgctaagc
    25501 gactgttttg ccgaagcaat tcaatccctc gattggttgc ttcaaaacct gcgttgtctc
    25561 cgtcatagca aaagaccaat tctttcgtca gcttttccat ttgttggatc tgttgagctg
    25621 ttagacttgt ccccatagaa gctaatccag ttttgatacc agcctgccat gcagcaatca
    25681 catccataaa cccttcgaac aacaaaattt ctccgttttt ccggatatcg cttcttgctt
    25741 tatcaagatt aaataaaact tgtcttttat tgaacagttc tgtttctgga ctattcaagt
    25801 atttgggctg atctttttcc tgcccttttt caggtgcaag ccagcgtcct gaaaaaccga
    25861 tcgtctggcc ttgagaatta cgaagtggaa acatcactct ttgataaaaa cgatcagaca
    25921 tacggccatc atcatgcgtg acaaacaatc cagagcgttc taacaattca tttgaaattg
    25981 attcattctt gaatacttgc tgaagaaaag aacgctcatt aggtgcaaaa cctatgttga
    26041 attcttcaat taattctaaa gacaacccac gactttgcaa atattctaaa gctggctgtc
    26101 cagcttttgt atgcaatagc atgtgatgat agatttccgc tgctttttca tgagcagcaa
    26161 tcagtttccc cgtagcagat tcaggatttt ggacagctat agtacgctga cgccattgat
    26221 cttctaacgg tatctgttcg aagtctgcca cttttaccac tgcttctgga aaacttagac
    26281 cttctagttc ttgaagaaaa gtaaacacgt ttccgccctt accacaacca aagcaatgaa
    26341 aaatctgctt gtcttctgca acagaaaaag acggtgtacg ttcttcatga aaaggacaca
    26401 gccccaagta atttttacca gactttttca actggacata ttgtccaatc acctcaacga
    26461 tattcgtttg actgcgtact gtttcgataa cctcttgagg aatccgttga gccatttttc
    26521 caccccctgc tctattgata tcttttgggt ttaacagaag atatttcagg agaataaccg
    26581 gtaaaaaatc gcactaactg aaatcagtgc gattttaaag gacacaatta tacatgtttg
    26641 attgtatcat atttcattgc tttttcaagt ataacacatt tctatctcat taagcatcaa
    26701 tttgctactc ttttcagtaa ctgattttga atatcagcct tccagtacaa tactcgaaga
    26761 acaatattta cgagcatgaa tacaaagaag tatcctacgc tctctcccaa ccctaagttt
    26821 acttccaaaa ttccatggaa cagcattgca gctacatata tccctacgat cgtcgttact
    26881 gctaggaaga tgcgcaatgg gttgaaggga taacaagctt ttacaactgc catgcaactg
    26941 atcccaatca gcaagtaata catcaaagta gtagtttcag catttcccca tgcgaatgtt
    27001 tttccaagta gatacaccgc aatgatattc actactacaa gcaaggcatt tggtaaagca
    27061 ttaataagtg ctgtaggcaa ataacggtaa gtgatttttc ttttatcttg ttcaaaagat
    27121 aaaaagaagg aaggataccc ttcaatcgct aaatcaatca gcgtgatttg gatagggata
    27181 aacggaaatc caattgctgt aacggcacat ataatcgata aaataaatga ataaatcgtt
    27241 ttgataaaaa agatgcctga gactttcgtc acattgttga ccactcggcg tccttcaaac
    27301 aggacttctg gcaatgtagt aaaatcagag tccaacaata ccagattcgc aatctgacgt
    27361 gttgctccat caccttctgc catagcaatg ctacaatctg cttctcttaa cgccaaaaca
    27421 tcattcacac cgtcgcctgt cattgcaacc gttctgccgc tttctttcaa ttcatttacc
    27481 agcaatttct tttgctgagg actcactcgt ccaaaaaccg tatagcgatg agcagcttct
    27541 cgcacttcca tttcttcagt aatttgagaa agatcgacat acgcatcata attcggtaat
    27601 cctgcacgtc tagcgatatt ggagacagta aaaggattgt ctccagaaat cactttcaga
    27661 tccacacctt cctcatgaag ataagctagt gtttcttgag cgttttttcg gattggatca
    27721 tcgatttcaa acagagcaac cggctcaaca aatgctggtt tttcttcgtt caattgctga
    27781 tttggagcga tccctaacat aagtacgcga tagccattcg cttgggctgt ttttagactg
    27841 tccggaagcc gatcttcttc caccaaacgt tcaggcgctc ctacgtacac gactcccagt
    27901 tctggaaatt ccatcgcgcc ccatttgcga tcagaagaaa atgcgacttt gtttgtcaca
    27961 tcataccgat tagaaagctc aaaataatct ctcatagccc gcatagtgat gttgttatca
    28021 ctgctttctg ccagatacgt ccccataatt tctgggatgg ctttctcgta cagcggatgt
    28081 agtgcttcta cccgctggat actcattttt ccttcagtaa tggtccctgt tttatccaag
    28141 cataatgtat cgacatgggc cagcgtttca attgaataca tgtcttgaac aaggattcgt
    28201 ttttttgcca gtttggttac ccctgtcgtt aatgcaatac ttattagcaa taccaaaccc
    28261 tttggaagca tgcctaacaa agctgctgca gatgcaataa cagaaatttt gatagaagca
    28321 tcgcggatga acagtgcttc caaaaatagt atgatcccca aaggaataat cacgaaacta
    28381 gtgaatttgg atactttgcg aatcgaatct acaagttcag aacgaatagc tttgtgagtc
    28441 tttgcctcgc tagtcaagcg gacagcataa ttatctgcac ctactcggat aacttcagcc
    28501 atgacagaac cgcttgtcaa aaagcttcca gaaagcaatt cagctccagt ttctttgaca
    28561 atcaaatcag attcgcctgt caaaagagct tcatttgctt ctgcccgtcc agatataacc
    28621 tgtaaatcgg ctggtatctg ttctcctgct ccgattttta ccaaatctcc taataccagc
    28681 tctttagtag aaatcgattg ttctttaccg tcccggatca ctagtacttc tgattgggaa
    28741 acaatcgata atttccgtac aagatttctt gcatgtattt cttgataaat tccaataacg
    28801 atattgacga caataattgc taaatacgcc atgttggaat acgcacctac aaataacaga
    28861 caaatcccaa taccaaagtt cagaaaattg aataacgtca atatattatc gctgaatatt
    28921 tgttttgtac tttttgatgc atcttcctgg tagtcatttt gctggccaga agccattttc
    28981 tgaactacct cttgctgtgt aagtccattc attttctttt tctccatttt ccgccaactt
    29041 tcaaaaataa tcacgtgttt aagtctagcg tgttccaaga ttaaaaacaa cttgttttta
    29101 taaaatttat agaaaaacac acgaatgatt ttacaaaaaa gatgccaacc tttttgtttg
    29161 aatcacccgt gtgctgtttt tattatcaat ctatctttac ttatattgtt cctccaaacg
    29221 gctcatccac caacctagtg ctgtaccggc tataaaaaga acaggacaag ctagtacccc
    29281 agcaactccc aatcccgcgt aatcggtagg aatgatcata atcgttgaag caaaaactac
    29341 acctaaaatg aaatggaata gcttgctata tgcctttgag aaaatgtaat ccatgatttt
    29401 tgacaatcca agaacacaaa gaatcccacc aattccgatc ggcacaatca cttcaagtcg
    29461 tacttcttta aatccgtcag ccatcgcttt atacaatccc atatacacca aaaaattaga
    29521 agggctcaat cctgggacga tcatacccaa accaatcaat ccgccggcaa tcagccatgt
    29581 tccaaaattc tgatcgactt gcccgtcaaa taattgagcg ccgtataata aaaacaaata
    29641 agccagaaca aaagtaacaa ccatgatgat cacatcagaa gtatctcttc cttttttccc
    29701 tgcttctttc cataacgcag gaacagttcc agcgatacat ccgacaaaaa accataatat
    29761 cgttgtttca tacgcaccta ataaaaaact gactaaaaaa gaaagtagaa agatgcctgt
    29821 cactccgcct agaccaacag gcagaaaaaa caaaacattt tctttgaaat tttttgtaag
    29881 gtgggctaaa aaagaaatca gccgctcata catgccaaag acagctgcta atgcaccacc
    29941 gctgactcct ggcaagataa atcctgaacc gataaacatt cccttcacaa accgtaacat
    30001 ccagtctttg gttgtgtacc ccactttttg attttccatt ttttgccttt tttccactat
    30061 ttgcgcactc cttctttcat ttaattttat tcatgattga acatttcctg ctattttact
    30121 taaaaacttt ttcataggtc tctattattt tttcattcat tttgacaata tcaggataaa
    30181 acatgttacc tccattatga tagagatagc cgccattgta aagcaaggac tggatccgcc
    30241 aatatcggta ttgtttccct tcctcatttc ctaaaagtgg gctcagcaca tctttagaat
    30301 acgcttctgc taattctact gtgttttctc cgccatgatt ttttacatag gcaatataat
    30361 ctttcccaaa attatatgct tggacagctg tccataaatc gcatccttgt ttctttgctt
    30421 ctactatcat ttccgccaaa tatttgacac cttgatcaat actgtcagct ggattatcaa
    30481 tgatctcagc ttctccatgc agactttccg agctccgcat gagatctgtc ccttttcctt
    30541 ttgattcggt caatattatc gccttgatca aattctcgta gttttccata tgataatttt
    30601 ttgcagctgt tgttatttct atttgatagc tttccactcg ttttaccgaa cgataagttc
    30661 ttcctcctaa aaacaacacc gcaccaataa ccgtcagaat gatcatgtaa aaaagaatac
    30721 ggataataga taatcgtttt cgcttcattt catcacctag ttattttgac atcttttttt
    30781 atcttactat ttctttatct tcttggctat ttcttccttg atttctgtgt acgccttaag
    30841 aaaaaatgaa ttttcctgta ttttttgtca tcttacgaaa atgatcgttg tttttcagta
    30901 aaaacaattg cccaatgact gatataatcg ttatacttta cacagtaggt gattatgtga
    30961 aaaaagaaaa gaaatatgca aaaatgccag atgggagcga aatttactat gaaaagagcg
    31021 gtcaaggctt ccctctcttt ttactgcatg gcaatgacgg cagcggccgc tttttttcag
    31081 aacaggtccc tgtactcgaa cgctactata ccgtgtatct agtagatagc cgaggacatg
    31141 gtcgttcgac aaacgaagca tctatgttga attttcagtt aatggccgag gatctgaata
    31201 cgatcatgtt gctggaaaaa atcgatcagg cagattttct tggatttagt gatggagcaa
    31261 atctagcact ggtatttgcc agcagcttcc ctaaaaaagt tcatcgattg attttaaatt
    31321 ctggaaacac gcttgttaaa ggcgttcgtt tttctgccag agtcatcagt aatattcatt
    31381 atgcatgggt atggttactc tctttatttc gcccaagtct tcgcaaaaat cttttagtta
    31441 tcaaactttt gctgcatgac atcgggctta cagaaaatga tttaaaaaaa atcaactccc
    31501 ccactttgat catcgtggga aaaaaagatg taatcaagct aaaacattct ttgtatatag
    31561 ctaaaactat accaaaagct tcctttgtgc ttgtgaaaga acaaggacat gaattagcta
    31621 gaaaagatcc tgaacgcttc aatcgagaag ttcttcaatt tctttctgaa acataactat
    31681 taggaataga aggtgattta tttgttaaaa aataccatac aatggctgaa aaaccaccta
    31741 ggactgttca aaacaatctt tttgatttct gtcattgtca ttattgtcgg tgaattgatg
    31801 tccatcggta aaactctatc tatccagcaa ttaggagaaa cgtttgccac tatacctgta
    31861 tggaaaagcg gacttatgct gttgattggt cttgtctctg tgttacctat gattggttat
    31921 gatattattt tgaatcggat gcttgaacaa aagcagaatc ctcgttactt gtttgagaca
    31981 agctggctga tcaatacaat caacaatatt gccggtttcg gcgggtttgt tagtgtgggg
    32041 cttcgctctg aactatatgg acaaaagaaa gagagcagaa acgtgatcca agcattatcg
    32101 aaagttttct tgtttttgat ggctggactt tctatttaca gtctgctttc ttttttactt
    32161 gtagtcttca cgccagttgc tccttttttg aagcaatatt ggatatggtt gatcggcggc
    32221 ggtctatatt ttcctgtcgt ctttttattt actactttca aaaagaaagg attccttggt
    32281 ggtacctcac ttaaaactca aggacagcta ttgctagttt cattgttgga atggaccggt
    32341 gtcctgttta gtttcctttc tgttggttat ttaatggaaa tccggattga tctgtggcaa
    32401 acaattccgt tattcattgc tgcttcagtg ataggaatcg tgtctatgat tccaggagaa
    32461 attgggagtt tcgatgtaat gatgattatc gggttgtccg ctattggtgt gccaagagaa
    32521 atagtcgttg tgtggatctt gctctatcgc ctgttctact acatcattcc gtttttgatt
    32581 ggaatcgtct ttttcttcaa aaatatcggt tccacttttg atcagcgtta ttctggtatc
    32641 cctaaacaat tagcaacaga aattgcgcat aaaatcgtgg ttgtcttatt atacttttct
    32701 ggaatcatgc tagtactttc tgccactatc ccgcaagctt ttacagagtt tcgctggctt
    32761 catagtttga acccattgaa atttcatttt attatccaat ttcctagtat actattggga
    32821 tttcttttaa tcattatggg cagagggatc gcagcacgtg tgaaacgagc atatttgcct
    32881 acaatcttct tgattgttct agctttactt tatgttttat taagtgattt tagtttcaca
    32941 ccggtggttt tcttgtctat tctgcttctg atcattcttg cttctaaaaa cgaattattt
    33001 cgggaacaat tgatctattc atgggagtgg cgtacgatcg atggaatcat tattggtgca
    33061 ctgtccttgc tttacatcgt tattggggta tacaatctgc cggatttccc tcatcggcgt
    33121 caccatttta tttccttttt cttatttcct tctgaaaaga tttggttttc gggattgttg
    33181 gccatcatcg ctgttagttt catgatcgtt ctatttgttc atttccttca aggagaaaag
    33241 aaacagattg gcgaagcctt taatgaagaa aaggccctga agatccttac aacttatgga
    33301 ggaaactccg atagtcaatt gatcttctta aaagataagc ggatgtttgc ctatgaaaaa
    33361 gacggtgaac caactgttct tctccaattc gcttgcttca ataataaatg tattgtaatg
    33421 ggtgatcctt ctggtaagaa agaagatttt ccagaagcaa tcgaagcttt tatcgaagaa
    33481 acagatcgac tatgctattt accagttttc tacgaaacaa gcgaagagat tgttatgatt
    33541 ttgcatgaat tcggctacga tttcattaag atgggggaag aagcttatgt agacctgaat
    33601 agttttacaa cttcagggaa aaaaatgaag ggcactcgtg ctgtcttgaa tcggatcgaa
    33661 cgagaaggat ttacatttga tgtcttacaa ccaccatttt ctactgagca aatgagtact
    33721 ttcaaaaata tctctgataa ttggttgggt tcacgaaagg aaaaagggtt ttctttaggc
    33781 tttttctctg aagattatct acaaagagcg cctattgcag ttgtcaaaaa tacccaagaa
    33841 gaaattgtcg cttttgcaac gatcatgcct acttacacga acaataaagt aggaacgatt
    33901 gatctgatgc gatatgatcc taaaaaagca ccttctggca gtatggattt cttgttcttg
    33961 catttgtttg cctatatgaa agaagaacat atccaatggt tcaatttagg gatggcccct
    34021 ctttcaaatg tcggaacttc cagaaagagt tttttacagg aaaggattgc atacttggtt
    34081 tacgagtttg gttctcattt ttattctttc catggattaa gagaatataa aagcaagtac
    34141 gctacaaact gggtctcacg ttatactcta tattctagag atagttggat tgcatatgtt
    34201 atgattgctt tgctgattat tgataatgcg ccagttgagc agacacaagg tagaataagt
    34261 ggtttgaaaa aatggatacg aagaaagtat taaaaactgc ataccgatag atgaacactc
    34321 tcatgatgaa taaaagagac tgaagtaaaa cgaaagttca tgcttctaca atctcttctt
    34381 atacaattga agaacaaaga gcggtccaaa cagaaatcct gtttggaccg ctcttttatt
    34441 ttaaacattt ttcattacca aaatattcga gtcgcgtttc ttatgcttcg atctctgtaa
    34501 cgatacctga acccactgtc cgtccgcctt cacgaattga gaatgtcgtt ccgttttcaa
    34561 cagcgattgg atggatcaag tcaacatcaa tcgtcacgtt gtcaccaggc atgaccattt
    34621 ctgtatcttc tggcaatgtg attgttcctg taacatcagt tgtgcggaag tagaattgtg
    34681 gacgatagtt gttgaagaat ggagtatgac gtccaccttc ttcttttgtc aacacgtaca
    34741 cttctgcttt gaattttgta tgtggagtaa tggaacctgg tttagcaagc acttgtccac
    34801 gttcgatatc gtcacgttgg atcccccgta acaacacgcc tacgttatcc ccagcttccc
    34861 cgtaatctaa cgttttacgg aacatttcta cacctgttac aactgctttt tgtgtttcag
    34921 gtttgatccc tacgatctct acctcatcac cgacacgaac agcaccacga tcaatacgac
    34981 ctgaggcaac agttcctcga cctgtaattg agaagacgtc ttccactggc aatagtaatg
    35041 gtttatctgt atcacgttct ggtgttggga tatattcatc tactgtatcc atcaattcca
    35101 taatagcagc ttcagcatct ggatcgcctt gcaatgcttt taatgcggaa cctttgatca
    35161 caggagtgtc atcgcctggg aaaccatatt cgctcaataa ctcgcgaact tccatttcta
    35221 ccaaatcgat caattcttca tcatcgacta aatcaacttt gttcaagaaa acaatcaggt
    35281 attttacacc aacttggcgt gacaataaaa tgtgttcacg tgtttgaggc atcggaccgt
    35341 cagttgcaga aacaactaag attgcaccat ccatttgtgc tgcccctgta atcatgtttt
    35401 tcacatagtc cgcgtgtcct ggtgcatcaa tatgcgcgta gtggcgtttt tctgtttcat
    35461 attctacgtg tgcggtattg atcgtaatcc cacgttcacg ttcttctgga gctgcatcaa
    35521 tactagcata gtcttgagga tttgctaggc cttttttgcc taatactgta gtgatcgcag
    35581 cagttaaagt agttttccca tggtcaacgt gtccaatcgt accaatgtta acgtgtggtt
    35641 tacttctgtc ataatgttct tttgccataa tttacaaacc tcctaaataa tatagatcac
    35701 tgaaaaattt cagctctcac ctttttatta tagtcttttt ttgactaaat caaaaagaat
    35761 acgtccgact tactttttgt aagattcgaa ctaagcttat tatacgtcaa tattggtcaa
    35821 agtcaactaa gagcactcgc ttgtcttata cgcgaaaaaa gtacctttta taaataaaaa
    35881 aatgtgtttt ttgagaaaaa cttcaagata gtccttaact ttcttttgaa gcaataagaa
    35941 caaaatgagg aaaagtttaa aattcagctg ttgccaacaa ctttcaatag acgaaatctt
    36001 ctatttcgtt aataataagg aaagaggtga agtacatgaa aaaattaatt ggcgtacttg
    36061 tcgttttagg catatttctc ggcggaagtt ttgcagctta tcattatttt tacggcggcg
    36121 aagcttatta tacaaaaatc acgacgaatg gcgaaatgtc agctacccaa gcagataacg
    36181 gagagaaatt cacgacttat acctacaagc aagcggctta taataaagat ggtgaaaaaa
    36241 aacaggtgac gcttcgcgaa gaaagagaac atccattaaa gatgaatgcg tatcttaaac
    36301 tcaaagtcaa tcctcgtaaa ggtgtattaa gctgggaaga aataaaggct tctgaagtgc
    36361 ctaaaaatgc ggcagaaaaa atcgataatt agtaaatggt cttaaaattt tattagataa
    36421 aa
//
LOCUS       DAZKCZ010000016        35602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000016 DAZKCZ010000000
VERSION     DAZKCZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.016"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..395
                     /locus_tag="ACG8KN_000674"
     CDS             27..395
                     /locus_tag="ACG8KN_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFP9187757.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            565..996
                     /gene="mraZ"
                     /locus_tag="ACG8KN_000675"
     CDS             565..996
                     /gene="mraZ"
                     /locus_tag="ACG8KN_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9187758.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1017..1976
                     /gene="rsmH"
                     /locus_tag="ACG8KN_000676"
     CDS             1017..1976
                     /gene="rsmH"
                     /locus_tag="ACG8KN_000676"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9187759.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1981..2382
                     /gene="ftsL"
                     /locus_tag="ACG8KN_000677"
     CDS             1981..2382
                     /gene="ftsL"
                     /locus_tag="ACG8KN_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9187760.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2383..4575
                     /locus_tag="ACG8KN_000678"
     CDS             2383..4575
                     /locus_tag="ACG8KN_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9187761.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4601..5563
                     /gene="mraY"
                     /locus_tag="ACG8KN_000679"
     CDS             4601..5563
                     /gene="mraY"
                     /locus_tag="ACG8KN_000679"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9187762.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5577..6938
                     /gene="murD"
                     /locus_tag="ACG8KN_000680"
     CDS             5577..6938
                     /gene="murD"
                     /locus_tag="ACG8KN_000680"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9187763.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6964..8052
                     /gene="murG"
                     /locus_tag="ACG8KN_000681"
     CDS             6964..8052
                     /gene="murG"
                     /locus_tag="ACG8KN_000681"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9187764.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8067..9287
                     /locus_tag="ACG8KN_000682"
     CDS             8067..9287
                     /locus_tag="ACG8KN_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFP9187765.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9443..10768
                     /gene="ftsA"
                     /locus_tag="ACG8KN_000683"
     CDS             9443..10768
                     /gene="ftsA"
                     /locus_tag="ACG8KN_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9187766.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10791..12032
                     /gene="ftsZ"
                     /locus_tag="ACG8KN_000684"
     CDS             10791..12032
                     /gene="ftsZ"
                     /locus_tag="ACG8KN_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9187767.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12049..12726
                     /locus_tag="ACG8KN_000685"
     CDS             12049..12726
                     /locus_tag="ACG8KN_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFP9187768.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12740..13339
                     /locus_tag="ACG8KN_000686"
     CDS             12740..13339
                     /locus_tag="ACG8KN_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFP9187769.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13420..13641
                     /locus_tag="ACG8KN_000687"
     CDS             13420..13641
                     /locus_tag="ACG8KN_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFP9187770.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13690..14472
                     /locus_tag="ACG8KN_000688"
     CDS             13690..14472
                     /locus_tag="ACG8KN_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFP9187771.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14493..15200
                     /locus_tag="ACG8KN_000689"
     CDS             14493..15200
                     /locus_tag="ACG8KN_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFP9187772.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15462..18260
                     /gene="ileS"
                     /locus_tag="ACG8KN_000690"
     CDS             15462..18260
                     /gene="ileS"
                     /locus_tag="ACG8KN_000690"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9187773.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18309..19835)
                     /gene="zwf"
                     /locus_tag="ACG8KN_000691"
     CDS             complement(18309..19835)
                     /gene="zwf"
                     /locus_tag="ACG8KN_000691"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9187774.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19850..20497)
                     /locus_tag="ACG8KN_000692"
     CDS             complement(19850..20497)
                     /locus_tag="ACG8KN_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP9187775.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20681..21010)
                     /locus_tag="ACG8KN_000693"
     CDS             complement(20681..21010)
                     /locus_tag="ACG8KN_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFP9187776.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            21187..21915
                     /gene="sfsA"
                     /locus_tag="ACG8KN_000694"
     CDS             21187..21915
                     /gene="sfsA"
                     /locus_tag="ACG8KN_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9187777.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21931..22944
                     /locus_tag="ACG8KN_000695"
     CDS             21931..22944
                     /locus_tag="ACG8KN_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9187778.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22944..24221
                     /locus_tag="ACG8KN_000696"
     CDS             22944..24221
                     /locus_tag="ACG8KN_000696"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9187779.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            24284..26986
                     /locus_tag="ACG8KN_000697"
     CDS             24284..26986
                     /locus_tag="ACG8KN_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9187780.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            27138..27455
                     /locus_tag="ACG8KN_000698"
     CDS             27138..27455
                     /locus_tag="ACG8KN_000698"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9187781.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            27485..27805
                     /locus_tag="ACG8KN_000699"
     CDS             27485..27805
                     /locus_tag="ACG8KN_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9187782.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27913..29373
                     /locus_tag="ACG8KN_000700"
     CDS             27913..29373
                     /locus_tag="ACG8KN_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9187783.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            29441..29662
                     /locus_tag="ACG8KN_000701"
     CDS             29441..29662
                     /locus_tag="ACG8KN_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187784.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29693..29875
                     /locus_tag="ACG8KN_000702"
     CDS             29693..29875
                     /locus_tag="ACG8KN_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFP9187785.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29875..30288
                     /locus_tag="ACG8KN_000703"
     CDS             29875..30288
                     /locus_tag="ACG8KN_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFP9187786.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            30411..31592
                     /locus_tag="ACG8KN_000704"
     CDS             30411..31592
                     /locus_tag="ACG8KN_000704"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9187787.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(31663..31827)
                     /locus_tag="ACG8KN_000705"
     CDS             complement(31663..31827)
                     /locus_tag="ACG8KN_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187788.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            31981..32054
                     /locus_tag="ACG8KN_000706"
     tRNA            31981..32054
                     /locus_tag="ACG8KN_000706"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32015..32017,aa:Arg,seq:cct)
     gene            complement(32123..33262)
                     /locus_tag="ACG8KN_000707"
     CDS             complement(32123..33262)
                     /locus_tag="ACG8KN_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9187789.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGLEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            33434..33568
                     /locus_tag="ACG8KN_000708"
     CDS             33434..33568
                     /locus_tag="ACG8KN_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187790.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(33561..34196)
                     /locus_tag="ACG8KN_000709"
     CDS             complement(33561..34196)
                     /locus_tag="ACG8KN_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187791.1"
                     /translation="MDNRNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(34309..34944)
                     /locus_tag="ACG8KN_000710"
     CDS             complement(34309..34944)
                     /locus_tag="ACG8KN_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFP9187792.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQIEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(34978..35439)
                     /locus_tag="ACG8KN_000711"
     CDS             complement(34978..35439)
                     /locus_tag="ACG8KN_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFP9187793.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        11741 a         6091 c         7345 g        10425 t
ORIGIN      
        1 caacgataaa aaaggagttt acgttcatgg gcacattcac accacttatc ttgttttggt
       61 atatttaccc ggtaatcgtt ttgtttggtt gccaatttct tgtaaaatta ttttcgttaa
      121 atcgtcgttt taaaataaaa gcccctgatt tggcgattcc ttttttgtgg ggcggtatgc
      181 atgcattgtc tagaaataca gggacaattt ctatcttacc gtttttcttt atttctgttt
      241 tgctaatggg aatttgtatt gctgttttcc aagcatatta ttatgaagaa attatttatc
      301 cgcgttattt caagatgact tggcgtttgg tttttctgtt gacaatcgtc ttgtacgctg
      361 tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa atagaaaagc tgtaaaaagt
      421 cttttaaaag gctttttgca gcttgatttt taaaaaaatg tttatgaata aaaaaagttg
      481 ttcccggatt tcccagaaaa ggtggtagaa agtgggggat tgtggtagac taattttagt
      541 tagtggagga atggggggct ttcaatgttg atgggtgaat tccaacataa tattgatgca
      601 aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac ttggcgaaaa gtttgttctg
      661 acacgcggat tggatggctg tcttttcggc tatccgatga gcgaatggga aaatctggaa
      721 gctaaactga acgaaatgcc gttggctaaa aaagacgcac gtacttttgt gcgattcttc
      781 tattcggctg ccactgaatg cgaacttgat aaacaaggac gaatcaatat acccagcacg
      841 ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca tcggtgtatc taaccgaatt
      901 gaaatttggg atgagacacg atggcaagat ttttctgcag cagcagaaga gaactttgac
      961 gaaattgctg aaagtatgat tgatttcgga ctataagaat aggaagaggc tgactaatga
     1021 ctgaaacatt tcaacactat acagtcatgt tgaaagaaac ggtagatgga ttgaacatca
     1081 aaccggatgg cgtttatgta gactgtacac tcggcggtgc cggacatagt gaatatttat
     1141 taagccaact aagttctaaa gggcatctgt atgcgtttga ccaagatcaa aaagcaatcg
     1201 atcacgccaa gctgcgttta gcttcttatg tcgaacaagg tatggtcaca tttatcaaag
     1261 cgaacttcag agaattagaa gaacgcttga gtgagcatgt agataaggta gacggcattt
     1321 tgtatgatct aggggtttcc tctccccagc tagatgaagc agaacgtgga tttagttatc
     1381 atcaagatgc accgttggat atgcgcatgg atcagtcagc tcctttatcc gcttatcacg
     1441 taattaatga atattcctac catgaattag taaagatttt cttccgctat ggcgaggaga
     1501 aattttccaa acaaattgct cgtgaaatcg aacgagtacg aaaaacaaaa ccaatccaaa
     1561 caactggtga gcttgtcgaa atcattaaac aagtcatccc agctcccgca agaagaaaag
     1621 gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat tgcagtaaat gacgaactcg
     1681 gtgccgaaga ggcctcttta gaacaggcaa tccggttatt gaaagtcaat ggaagaatca
     1741 gcgtaatcac attccattca ttggaagaca gaattgtcaa aagtatgttt aaagaatata
     1801 gtacggtaca agatctacca ccgggacttc ctgtcatacc tgacgaattc caaccagaac
     1861 tgaaagtcat tactcgtaaa ccaatccttc cttcagagga agagctaaca gaaaacaatc
     1921 gatcacgcag tgcaaagtta cgtattgcag aaaaaataag aatgaaagag gagtagaatg
     1981 atggcagaat taaagaaaat gcaagattac gaatatgatt tacctgaatt ggtggatcag
     2041 ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa tcattcttcc acaatcaccg
     2101 gcaaaacgat taaagcagat ttctgctttg gaaaaaattg ctgtggcaag cgtggcgatt
     2161 gctattgttg ctttatgtgt tttaacagtt atgctgcgca caaatatcag cggagctgaa
     2221 aaagaaatca cgtcaataca aacagaaatc aatgataaaa agcaagaaaa aacgagttta
     2281 gaacaagaaa aaaacgaact ttctcgtact gaacggatta aacaaatcgc agaagaaaaa
     2341 ggattgtcta ttaatgacga caatttaagg aaagtgaagt aaatgagtct taaaaataaa
     2401 attcggcggt ttatggaaaa gaaaaatcta aatccgatga acaatcgcaa aaaagtaggt
     2461 atcattttat ttgctacgag tattggattg ttctttttat ttgccgtccg tttctcgtat
     2521 atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa acagttgtac
     2581 caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa cggtgtggcg
     2641 ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac atatacctct
     2701 ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga aatcctgcat
     2761 aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg cgcaaaaaaa
     2821 gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga aaccaagcaa
     2881 aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt cgaccatcag
     2941 gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc agttccagac
     3001 aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa tgatatcttg
     3061 agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc tctgccaggt
     3121 acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac tttagacagc
     3181 agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata tcagccagaa
     3241 gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat gggacaacga
     3301 cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat ctggcgtaat
     3361 ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac tactgcagct
     3421 gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa aatccaagta
     3481 gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac catgcgtcaa
     3541 gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt aggcggacga
     3601 tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg attagatgat
     3661 gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag tgcttatgga
     3721 caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat tgcgaataac
     3781 ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac tggagaagag
     3841 cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac agaaaaagtc
     3901 cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtattgctta tggtgtatat
     3961 agtgtacctg gatacaatgt atcggcaaaa accggaacag cccaaatcgc ttctgataca
     4021 gggggatatc aaacaggaga tacagcctac ctgtattcga ttgtggagat ggttccttct
     4081 gaagatccag actatgtctt gtatctaaca atgaaacacc ctaagacata tgatcgaatg
     4141 gcattggcta aaatcgcaaa tccattgatg aaacgggcaa tggattttaa agaaactgaa
     4201 gaagattcag atacagaaac aaaaacagaa aaagtatccg tagccgatta tcgtaatctt
     4261 gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac tgcaacctgt agtgatcggt
     4321 aatggtaaaa aagtacaaaa acaatctact gccaacggtg atcagttgat ctctggagaa
     4381 aaactgatcc tatatactgg tggtgataag ctgatgcctg acgtaacagg atggtcaaaa
     4441 gcagatatca tgaagctggg gaaaatcctc ggtattgaag ttagttttga tggagatggt
     4501 tattgtgtca aacaagaact tgctccatat gaaaaaatca ctgaagacaa acttagtttt
     4561 actttagaag aataagaaga gaaataggag agaaaagagc atggaatgga cacaagcatt
     4621 gattcccatt gtcagcagtt gtgcaatgac aattgccgca atgccgctat ttattggtta
     4681 ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag ggaccaaagt ggcacaatag
     4741 caaagccgga actcctacaa tgggcggctt agtgtttctg atcggctcta ttttgacagg
     4801 aatttgggtt ggagcttggc aaaaacagct aactcctact cttttcatct tgctttttgt
     4861 attagcgtta tacggagtaa tcggctttct tgatgatttt ataaaaatat tcaagaaaag
     4921 aaacatggga ctgaactcga agcagaaatt gattggacag attctagggg gaatcatctt
     4981 ttatcttgtt taccgttctg aaggttatcc aggtgtattg aatttcttcg gactagatct
     5041 acctttagga atcatttatg gtattttcgc tctcttctgg cttgttggat tttcaaatgc
     5101 agtaaatctg acagatggca tcgatggatt agttgctggc ttaggaagta tctcttttgc
     5161 agcttacggt ctaattgctt ggcatcagca tcagtatgat gtattaatca tttgcttaag
     5221 tgttttagga ggtttactgg gtttcttcgt ctacaacaga aaacctgcca agatttttat
     5281 gggagatgtc ggttcattag cgttgggcgg acttttagcg gcaatttcta taatgctaaa
     5341 ccaagaatgg acattgcttt tgattggttt gatctatgtt atggaaacag caagtgtgat
     5401 gcttcaagtt acttccttta agcttacagg aaaacgaatt tttaaaatgt ctccaattca
     5461 tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt gatacagttt tttggttgat
     5521 cagcattgtg acttcgttga tcacattatg gatcgtttgg taggagatga cgggaaatga
     5581 aaaaaatatc aacatatgaa aataagaaag tgcttgtctt aggactcgca aaaagcggag
     5641 taagtgcggc caaattatta catgaattgg gcgcacttgt caccgttaat gatggaaaac
     5701 cttttgacga aaatccggaa gcacaagagc tgctttcttt agggataaaa gtaataacag
     5761 gtagtcatcc aatcgaattg ttggatgaag aattttcatt gatggtcaaa aatccaggga
     5821 ttccctacag tcatccttta gtagcaaaag cccaagaaat gggtattccg gtaattacgg
     5881 aagttgagct agcttacgaa gtagcggaat gcccaattat tgggattaca ggaacgaacg
     5941 gaaaaacaac gacaacaacg atgacaggac tactactgaa tgcaggagca gatcaaggaa
     6001 ttgcacgttt agcgggtaat attgggtatc cagcaagtgg tgtagcgcaa gaagcaaaat
     6061 cagaagataa aattgtgatg gaattgtcta gcttccaact catgggaatc acagatttcc
     6121 gccctcatat agcggtgatt accaatatct atgaagcaca tattgattat catggtacaa
     6181 gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa catgacagaa aaagattatc
     6241 tgatccttaa ctggaatcag tcagaactac aagaattagc gcaaagaaca aaagcacgcg
     6301 tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt gtatgctgac gattactcaa
     6361 tatactataa aaaagaaaaa ataatggaaa tatctgaact tggcgtacca ggtaagcata
     6421 atgtagaaaa tgcactagct gctatttctg tagcaaaatt gtatggtata tcaaatgaag
     6481 ctatacgaga aacgttgcat tttttccatg gcgtaccaca tcgtacgcaa tatgtaggag
     6541 aaatccaagg cagaaaattt tataatgatt caaaagcaac caatatttta gcgactaaaa
     6601 tggctctaag tggatttgaa acgtctaagg tcgtgttatt ggctggcgga ttagacagag
     6661 gaaatacatt tgatgaatta attccttcat taaaaggaat caaagcaatg gtcgtatttg
     6721 gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc aggcatcgag acaatagtaa
     6781 cagccgattc tgtagaacaa gcagttcctt tagcattaga aaacagtaca gatggtgatg
     6841 tagttttatt atcaccagcg aatgccagct gggatcaata cccaaatttt gaaactagag
     6901 gaaacagatt catggaagct gtgaaccgat taaaatagaa aaagtagaag cgagcgataa
     6961 aaaatgaaaa ttttggttac aggcggagga acagggggac atatttatcc agctcttgcc
     7021 tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat tcatgtatgt gggagcaaaa
     7081 cgaggtctag aaaacaaaat cgtccctgat acaggaatgc ctttccatac cttggagatt
     7141 caaggattta aacgaaaaat ttccatgcat aacatcaaga cggtccgatt gtttcttaag
     7201 agtatccgtc aagcaaaaaa aatcctcagt gattttcaac cagatatcgt tatcggtact
     7261 ggaggatatg tttccggagc ggtagtgtac gctgcagcca aaatgggtat tccaacagtt
     7321 atccatgaac aaaacagtgt accaggtgtt accaataaat ttttaagccg atatgtggat
     7381 cggatcgctc tttcatttaa agatgcggct tccttttttc ctgaaaacaa agcagttctt
     7441 gtaggtaatc ctcgagcaca agaagtagca gatactaaaa aatcagatat tttgaaaaca
     7501 tttgatttgg atccagaaaa gaaaacagtc ttgatttttg gcggtagtca aggagcactt
     7561 aaaatcaatc aagcagtatc agttttttta agtgaatata ttccttcttc ttaccaagtt
     7621 ttgtatgctt caggagatag atattatgaa gagatcctaa ataaaataaa tcctgaaata
     7681 caaaatatta gtgtccagcc ttatatcaaa aatatggcag aggtcatggc ttccagtgat
     7741 ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat tcacagctct ggggttaccg
     7801 gcagtgttgg ttcccagccc atatgttacg aatgatcatc agactaaaaa tgcaatgagt
     7861 cttgttcacg caggagcagc aaaaatgatt gcagataatg aactaacagg tgaatcacta
     7921 agtcagacag tcaacgaaat catgggtgat gaagaattgc aaaagcaaat gtgccgagct
     7981 tctaaagaac aaggaatccc tgatgcttca aaacgtcttt atgacttagt aaaacaaata
     8041 atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa agaaccaaaa aaattgtctc
     8101 atccgtcttc tgatgaaaac aatttgactc cttggcaaaa ggctaaccga aaatatctgg
     8161 cagaacaaga gatgaaaaac caagagccaa aaaaagaaga cataacatta gatacacccc
     8221 tttcagattc tgaagaagaa aaaacggttc aagaggaaca gcaagaagaa agccagtccg
     8281 agaaaaaaat cgaatttttt gaagagatca agcaaaagaa aaacggtgga ccatataacg
     8341 gttctttttt gaaccggctg cccaatttaa aaagtcaacg gcacaaagta ctatttcgtc
     8401 ggttagcttt gatcatcact gtattgggta taccgctgat ttttttgatc tattatgttt
     8461 ctccttatag caaattacaa gcagttgcag tatcaggcaa taagacagtc aacagccaag
     8521 aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt ttggggacag tattttcatc
     8581 gatctactta tatcgatcgc cttaaaaaag cacagccaag aatcgaaaca gcaaatatac
     8641 atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga atataaagag attgctttga
     8701 tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg aacagtatta gatgaaaaag
     8761 tagcaaatcc tacaaaaaat ctccctatct tggaagagtt caaagactct gcaaaaatca
     8821 aagaattaac aaagcaatac aatcagttat catcagaatt acaaaaagca atttctgaaa
     8881 tcaagtacac gccaagagca agcaataaaa atctgatcca attgaatatg aatgacggca
     8941 atcaagttat tgtaaatatc aataatcttg cgaaccaaat gaaatattat tcgcaagtag
     9001 ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt tggtatcttc tcgcatcctt
     9061 acggtagcac tcaagaaacg gaagaatcgg gccaatcgga agaaacaact gaaagtgaag
     9121 aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga aacgcaaaaa gcacaaacac
     9181 ctggatcgtc tagcacaaac ggatcatctg attcaaatga ttcaagtgaa acaagtcaaa
     9241 tagaagataa tccaagtaca actccgtcaa gtttaaatga aaattgataa aacaacttta
     9301 aaaagtaaga atgtactttc taaaaaccag taatttatgg tagaattgga agcagtgaat
     9361 atatctatta ataagtatat tttaaaacgg acgctagttg ttcgcttgta tatggaatag
     9421 taagtaggag ggaatcccat tcatggcaaa aacaggaatg tatgtaggcc ttgatattgg
     9481 aacgacatct gttaaagttg tcgtggctga atatatcgac agccaaatga atattattgg
     9541 agtaggaaac gcaaaatcag aagggattaa ccgaggcatt atcgttgata tcgataaaac
     9601 ggtccaagcg atacaacgag cagtacgaca agcagaagaa aaagcaggta tccagatcaa
     9661 aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta gaaaattgcc agggaatgat
     9721 tgcagttaac ggagattcaa aggaaatcac cgatgaagat gtcagaaatg tggcttcagc
     9781 tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg tcaatattgc cacaagattt
     9841 tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga atgatcggcg taagattaga
     9901 aatgtacggt ctgttgttta caggacctaa aacgatcatc cataacatac gtaaatgcgt
     9961 ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact cctttagcgc tgactgaatc
    10021 aattctttct gacggagaaa aagattttgg tacgatcgtc attgatatgg gtggcggcca
    10081 aacaacaacg gctgttatgc atgataagca attgaagttt accaatcttg accaagaagg
    10141 tggcgaattt gtcactaaag atatctctat tgtattaaat acgtcattta acaatgcaga
    10201 agcgttgaaa ataaactacg gggatgcata cccagaacgg acatcaccag atgaagaatt
    10261 tccagtagat gtgattggtc agtcagaacc agtgaaagtc gatgaacgct acttgtcaga
    10321 aattatttca gctcgaatgg aacagatttt caacaaagca aaagatgcat tggaccaaat
    10381 cgaagcatta gagttgccag gtggcgtcgt attgacaggc ggagctgcta gtcttcctgg
    10441 tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta aaattgtatg ttccaaatca
    10501 catggggctg agaaacccgg tatttacaaa tgtaataagt atcgtggatt attcagcaaa
    10561 cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg ggtgaaacct cagcagctcg
    10621 tcaaatggta gttgaacaag aaacaacaaa cacatatgag agctatgaag cacctgaaga
    10681 aacaatctac gatgagccgg aaccaaaaga gtccggagaa aatgtaaaaa ataaaatcaa
    10741 gggtttcttt acaaatattt ttgattaatt catcctggga ggaataagat atggaattct
    10801 caattgataa taatatcaat gacggcgcag taatcaaagt catcggagtc ggtggtggcg
    10861 gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa aggtgttgaa ttcattacag
    10921 ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga aacagttatc caattaggcc
    10981 ccaaatatac tcgtggttta ggtgctggtt ctcagccaga agttggacaa aaagcggcag
    11041 aagaaagtga acaatcctta cgcgaagctt tagacggtgc agatatgatt ttcatcactg
    11101 caggaatggg tggaggaacc ggtacaggtg ctgcaccaat cgttgcaggt atcgcgaaag
    11161 aattgggcgc attgactgtt ggtgtcgtga cacgtccatt cacttttgaa ggaccgaaac
    11221 gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga aaatgtggat acattattaa
    11281 ttatctcaaa taaccgttta ttagaagttg tagataaaaa gacaccgatg cttgaagctt
    11341 tccgtgaagc agataatgtt ttacgacaag gtgtacaagg tatctctgat ttgatcacag
    11401 cgccaggtta cgttaacctt gactttgctg acgtgaaaac agtgatggaa aaccaaggta
    11461 cagcattgat ggggatcggt gtggctagtg gagaagatcg cgtagtagaa gcaacgaaaa
    11521 aagcaatctc ctctccattg cttgaaacat ctattgatgg agctgaacaa gtacttctta
    11581 acatcacagg cggtttggat atgacattgt tcgaagcaca agatgcttca gatatcgtag
    11641 caaatgcagc aactggcgat gtaaatatca ttctaggtac atctatcaat gaagaaatgg
    11701 gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga tgaatcaaaa aaggaacgca
    11761 agtctagccg accagctaga caagcacaaa tgcaatcacc tgctcaaaag actgtattag
    11821 atatggatca agctaaacca acttcttcag aagaagaaaa cagttttggc gattgggata
    11881 tcagacgtga acaaaatgtt cgtcctagag ttgatgattc caattttgac aatatcgaaa
    11941 aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc tcatggcgat gatgaattaa
    12001 atacgccacc gtttttccgt cgtaaaaaat aaaggagagg aagagcacat gattgctgac
    12061 aacttgcaga aaatcaagca agaaattcag gattcgtgtg ctcttgttca tcgtgatccg
    12121 aaagaagtta cattagtagc tgtaacaaaa tctgttgaat ccgctcaagc tgaagaattg
    12181 gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag ataaattatt agaaaagaaa
    12241 caagatttat ttgattttcc tgaaataaag tggcatttaa ttggtaattt acagcgcaga
    12301 aaggtaaaat tgattgtaaa tgaaattgat tactttcatg cgttggacag tttaagatta
    12361 gcagaagaaa tccagaagcg gttagagaaa gaattatcct gttttattga agtaaatgtt
    12421 tctggtgagg aatcaaaaca tggaatttca ccagatgagc ttttaccgtt tgtggaatct
    12481 ttagctgcct gcacaaaaat caaggttgtt gggttgatga ctatggcgcc taaagaagct
    12541 tctgatgagc aaatccataa agtgtttggt acgctgaaag aattgcgtga tcttgttcaa
    12601 gcaaaaggtt atgcccatgc accatgtagt gaattaagta tggggatgag tcaagacttt
    12661 caaattgcga ttgaagaagg tgcaacattt atccgtgtag gaacagcgct attcaaagac
    12721 gaataaagga ggaacaaaaa tgccgctttt aaacagagga gcaattgcta attttttcgg
    12781 attagctgat gaggaagagt acgaatacca agattatcct gaaacaacaa aaaaggctgt
    12841 acagccaact aaaaaaaata caacacagaa tcaatcaact cattctgtat ctgctgcgaa
    12901 tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta acgcatgaaa gtattcagcc
    12961 ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct acaaaaactg tagcgaaaga
    13021 aagaacagct tcaagaacct ctacacaacc taaagcaaat cttgctggaa aaatcactgt
    13081 aatggaacca agagcttatt ctgaagctat ggcgattgct aaacgaatca ttgctggaga
    13141 agcaacctta gtgaattttc atttagtaga agaaaaacaa gcgagaagaa tcgttgattt
    13201 tctaacagga acagtttatg cactagatgg agatatcaaa cgtgttggag atgagatttt
    13261 tctttgtaca ccgactggga tggagattga cagttcaacc gctcaatctt ttgcagatgc
    13321 gaatttgttt gatctgtaag aggaggaact acgatttata ctattgcact attacttcat
    13381 ttacttcaac gggcagccta tttctatagc attatcttga tggtatatgc attgctctct
    13441 tggtttccag gcgggtatca atcagggcta ggacgattat tagctaagat ctgcgaaccg
    13501 tatatcagtt tgttcgatcg tctgcctttg aggattggac ccttggattt cacaatttta
    13561 ggggcaatcc tgatcttgaa cttagccgta caaggtttag cacagatcgt tatatggata
    13621 actgtccatt tagtttattg attgaagcaa caaaatcttt attctcagaa tagataggag
    13681 gggaaaattt tggatgcaaa tgtataccag cactttcggg cagatgaacg tacatttatc
    13741 gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta tctgaccgaa
    13801 tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag cagtgatttg
    13861 cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt gattttcccc
    13921 gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat ccattatcct
    13981 agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg aacggggatc
    14041 aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt atttattgca
    14101 aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt tgctgtacgc
    14161 ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac acaagaaaga
    14221 ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa tatttccaga
    14281 cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac agaaaataca
    14341 agacctgatt ttgtacttga actattagat attatttcag tcagaggtta tggcagagtc
    14401 cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact gttcggtgta
    14461 ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta gatatccaaa
    14521 ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat gatttcttag
    14581 atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta gaaaaagcag
    14641 tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct ttgaaccaat
    14701 caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc aaggaatccg
    14761 aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac gctgaaaaac
    14821 gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg acagatgcaa
    14881 cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa acacgagtat
    14941 tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa agtccggaat
    15001 gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa gtgatcaaag
    15061 aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca gacacaggag
    15121 tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg atcgtgcctg
    15181 gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc gagccgatga
    15241 gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga cctgcttttc
    15301 cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag tttgcactag
    15361 atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg ctgaacatgc
    15421 ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg aaagaaacct
    15481 tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga gaaggcgagt
    15541 ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg aacgaaggaa
    15601 aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc catttaggac
    15661 attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg tctggctatc
    15721 gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa gtattggcta
    15781 ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata cttggaaaaa tgtcgtgaat
    15841 atgcactttc ccaagtagat aagcaacgag cagatttcaa acgcctagga gttgcaggag
    15901 attgggaaca tccttatgtt acattagatc cttcttatga agctgcacaa gtacgcgtat
    15961 ttggtaaaat ggctgaaaaa agctatattt acaaaggatt gaaaccaatt tactggtctc
    16021 cgtcaagtga gtcttcttta gcggaagctg aaattgaata taaagatgta aaatctccat
    16081 ctatttttgt agctttcaat gtcaaagacg gcaaagatat tcttgatgaa gatacagctt
    16141 ttgtcatttg gacgacaaca ccatggacat tgccagcaaa ccaaggtatt gctgtaaatc
    16201 caaaatttac gtatgtattg gtggaagccg atggccgtaa gtttgtcgta gcgaaagatt
    16261 tgctagaaac agtccaatca gaaatcggtt gggaaaatgt aacagttcta aaagaatttg
    16321 ctggacaaga aatggaatat atgactgcaa gtcatccatt ctatgaacgt gaatcacttg
    16381 tgattttagg cgatcatgtt actttagacg ctggtactgg attagtccat actgctccag
    16441 gacatgggga agatgactat atcgctggta gcaaatataa actatctgta gtgtcacctg
    16501 tagactcaaa aggattgttt acagaagaag cacctggatt tgaaggtatc ttttatgaca
    16561 aagcaaatcc tatgatcact gatctattaa aagaaaaagg agctttgctg aagctagatt
    16621 tctttactca tagttatcct cacgattggc ggacgaaaaa gcctgtgatc tatcgggcaa
    16681 ctccacaatg gtttgcatct atcgataaat tccgtcagaa tattttggat gaagtagaaa
    16741 aagtagagtg ggttatccca tggggtaaaa cacgtttgta taatatgatc cgtgaccgcg
    16801 gcgattgggt aatttcccgt cagcgtgcat ggggagtacc acttccgatt ttctatgctg
    16861 aaaatggaga agcaatcatc acaccagaaa caatcgaaca tgtggcaaaa ttatttgctg
    16921 aacatggatc aattatctgg tttgaaaaag aagcgaaaga acttcttcct gaaggattta
    16981 cccacccagg ttctcctaat ggcgaattta caaaagaaaa agatatcatg gatgtctggt
    17041 ttgattctgg ttcttctcat gaagctgttt tacgtcagcg accagaatta tcattcccag
    17101 cagatatgta tttagaagga tcagaccaat atcgtggctg gttcaactca agtatcacga
    17161 ctagtgtggc tatcaatggt gtagcaccat acaaatcagt tatttctcaa ggatttgctt
    17221 tagatggtga aggccgtaag atgagtaaat ctttaggaaa catcattgca ccagaaaaag
    17281 tgatcaaaca atttggtgca gatatccttc gcttatgggt atctagtgta gattatgaag
    17341 cagatgttcg tgtttctatg gatatcttaa gtcaagtggc agaagtatat cgtaaaatca
    17401 gaaatacgat gcgtttctta ttagcgaata cagaagattt cgatcctaaa aaagatactg
    17461 tttcgtacaa tgatttacgt tctgtagaca aatatatgac cgttcgattg aaccaagtaa
    17521 tcaaagaaat ccgagaagaa gggtacgaca aatataactt catgcatatt taccgtacag
    17581 ttatgaactt cttgacggtt gatctatcgt cattctacct ggattttgct aaagatgtag
    17641 tttatattga agcagaagat gattatcaac gtcgttgcat gcaaactgtt ttctatcaaa
    17701 cagctgtagc tttgacaaaa ttattgactc ctatcattcc gcatacagca gaagaaatct
    17761 ggtcattcct gaaagaagaa gaggagtacg ttcagttaag cgagtttcct ggttatgaag
    17821 agtttgcaaa ccaagaagaa ttgatggata cctggactgc tttcatggac ttccgtgata
    17881 atgtcttaaa agcattggaa gaagcaagaa acagcaaatt gattggtaaa tctttggaag
    17941 caaaactaac tgtttatcca aaacaacaag ttcgtgaaat gttaaaagct ttggatgcag
    18001 atattgcaca attattgatc gtttcaccag actactttga agttatggca gcagatgaac
    18061 aagtgccaga taacgctatg gtatttgatg atgttgctat tacagtggaa aaagcagacg
    18121 gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt aggagtagat gaaaaacttc
    18181 ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt ctatcctgaa gctgtagctg
    18241 aaggattcga agaaaagtaa aagggaaaag ggggtgtgac atgttctttg tcacaccccc
    18301 ttttgttatc aatcgtttaa ttttccacgt tcacgatacc agttatctgg ttgccagatc
    18361 cattcgaatc cgtctttttc aagtagttcg aaagctgctt taggtcccat tgatccagct
    18421 ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt ttcgaatgac atccacgata
    18481 tgccaagatt gagcgacttc ttcccaatga gagaagttcg ttccatcacc attcaacgca
    18541 tctaatagta atttttcgta agcttctggg ctattttcaa ccatttctgc actatttcta
    18601 aattccaatt tgattggttc cgtttcaaat ccttgcccta cttctttccc attcaaggat
    18661 aaagaaaatc cttcagttgg ctgaatatag atcgttagaa cattaggtgg aagtgtcgta
    18721 tcgtcacatg gttcatcgac agatgttttg aatacattaa caggaacttg tttgaatacg
    18781 atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg tacgtacata gaaaggaaca
    18841 cctgaccaac ggaagttatc gatagtgaat ttgcctgcga cgaatgtttc agtctctgat
    18901 tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt gtacaccatc caattcacct
    18961 tgagcatatt gcccgcgaac gaagttttct aacacttctt cttcgttata aatgcggata
    19021 gctttcaatg ctttgatctt ttctgtacgg atttcttttt cggaaaatgc tactggcggt
    19081 tccattgcaa gtaatgctac aacctgtaaa atatgatttt gcaccatgtc tttaagtgcg
    19141 ccactatgat cgtagtagcc cccgcgatct tctacaccga ggttttcgcc aaaagtaatt
    19201 tggatattgt cgatatagcg attgttccat tgagcctcaa aaatattatt ggcaaaacgg
    19261 atagctgaga tgttttggat catttctttt cctaaataat gatcaatcct gaaaatatct
    19321 tgttctggga atacttctct gatttgctgg ttcaattcaa aagcagactg gtaatcagaa
    19381 ccgaacggtt tttctatgat caaacgattg aagccatttt ttgtcataat attttgtgat
    19441 ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca tagccaaata atagacacgg
    19501 ttaccgttta attgatagtg tccatctaat cggtcagaca attcttttaa tgtctgataa
    19561 tgcgttgaat catttacatc atgggattga tagtaaaaat ggcttgcaaa ggtagttgct
    19621 tcttcatctg aaggattcaa tgcacggatt gtttctttga caacttcacg gtagtgctca
    19681 tcgctccagg gacggcgagc ggttccgatt acagcaaaat gctcgcctaa atttcctttt
    19741 ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg ctaggtcacc tgtaccgccg
    19801 aatatcgtaa acaatacatt tttttcatta gacatgtgga tacactcctt tatattttat
    19861 cgacaaaaat cgtgctggcc gccttaaagc tgacggggat tttctttcta tcattttgga
    19921 tagtgatcgg tccttcataa gctgcgatgt caacgatctc atattcttcg ttgatattca
    19981 ggtcaatagt gactaagtaa tccaacaatt ctttttcatc aagcactcgt gcgatacgca
    20041 cttttgtacc gacaggatag gactccaatg tttgtcggcg ttcttcatgg acaacttcct
    20101 cttttttagg aatcgatcct ccatgaggac aatgttcagg atgattcaaa tagttttcta
    20161 aatgatctgc aagatgttcc gaagtcacat gttccaatac ttcagcatca tcatggactt
    20221 cattccagct atagtctaaa tgttcaacta aaaaaacttc ccataagcga tgtttgcgaa
    20281 tcagactgct agctttttgc agtccgcttg cagtcaattg tacaccttga taaggggaat
    20341 gttctacaag ctcttctttc acaagtttag aaatcatttc actgacagat gcagctgaga
    20401 catctagact agatacgatt tgtttattgt tgactttcgt tgtatctcca cctagttcta
    20461 aaatgatttt caaataatct tctcgatttg gtgtcatatt cgatcatcct ttcatggtaa
    20521 aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt gactattttt ctaaataaaa
    20581 aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa gatgatagag aagactcatt
    20641 ctttcgttta taaaggatat atatatacat tgggagggat ctatttttca gataacaact
    20701 tatatgaagt gtctgcagat tcgtcataaa gagtttgttc gggataagaa aagataagat
    20761 aaaaaccata ctgatcacgt acgactaaac gatattcatc tatttctacc acagatgtgc
    20821 caagagactg accgttgatc aaaaacgtgt aatcagatgt aatctgtaat tgttgttttt
    20881 gctggctatc actaaagaac catgtacctt gatactttga gagtccttct gacttttccg
    20941 cttgtttttt ctttttcaag taaataaaac ttgagagaac ggtaatgagt gaaccaagaa
    21001 aatagatcat tttttttatc accaaatcac tcctttatga gaaaataatc gcatagacat
    21061 attctttctt ctttatttca ttgtaccgaa taaaagctaa aatttctcta agactttcga
    21121 ctggtttttt tactgttttt ctacaaatta tgataggata catagtagga agaaaggtga
    21181 cataatttga agtatcagca agtggaaata gcttttttta ttgaccgccc gaatcggttt
    21241 atagcacatt gtatgaaaaa agacggagaa ataattattg cgcatgtcaa aaatacaggt
    21301 agaggaaaag aggtcctgat accaggcgct gaagtggcta ttgtatttgc tcctggacct
    21361 aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga agggtgactg gtttaatatc
    21421 gatagtcaat tgcctaatcg gctggcaatt gatggtattt tagacggcac gatcctttta
    21481 ccaggattaa ctggtccatt tacggtatac aagcgtgaag taacttatct tcactccaaa
    21541 tttgatattt atttagagac ggtggtcgga caaaaagcat ttgttgaagt caaaggtatg
    21601 acacttgaaa ataaagcgat tggtgctttt cctgatgcgc ccacactcag aggattgaaa
    21661 catataggag aattaacgta tgccgcacaa gatggttatg ccgcttatgt cttgtttatt
    21721 gctcagttcg aacatcttca tctggcaacg atccatgaag aaatgcaacc agcactagct
    21781 gatatggttc gacatgcgca gcaaagcgga gttcaaatcc tcgcctataa ttgtcaagta
    21841 tcggcagatg aggtaactgt caagcaggcc attccatttg atgtagatat cccttttgaa
    21901 gatcctatac aatagaaaga aggtaaaata atgatcaact taggtattat cggaacaaat
    21961 tggattactc atcaatttgt ccaagcagct ttaagcacaa aaaaatatga attgacagcg
    22021 gtatattcta gacatttgga aaaagcacag gaatttggtt ctcattatga aggggatatc
    22081 gaattcgcaa cagatttgaa gacttttttc aatattactc atatggacac catttatatt
    22141 gcttctccga atagtcttca ctttagtcaa gctaagcaag caattttggc aggaaaaaat
    22201 gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa tggatgaaat catccatttg
    22261 gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa atatccatga atcaggtttt
    22321 aaaaagattg ccgattactt accattaaaa gacgagataa tcggtgcaaa tttcagctat
    22381 atgaaatatt cttcgcgcta tgaccaagta ttagatggag aagaaccgaa tatcttctcg
    22441 actcatttct caggtggtgc attgatggat ttaggtgttt accttgtata tgcagctgtt
    22501 gcttggtttg gaatgccaaa agaagtccat tatttcccaa gaaagatttc tactggtgta
    22561 gatggtcttg gatgggggat cttacgttat gagaattttg atgtatcgat ccagccaggt
    22621 aaaatcggtg attcttattt accgtcagag atttattttg actcaggaac gctagtgatg
    22681 aatggagtca atgcaattga acaagcacaa tttcatgatc gcgcgcataa agaaatcatt
    22741 gatttggcgg taacagctgc agaaaatcca atggcagatg aagcagagga ttttgccaat
    22801 gtcatggctc atccgaaaga tccagcttgg ggattgttgt atgaagagtg gatcgagcta
    22861 gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa atggcggcat caaattcgat
    22921 gcagataatg aggaagattt ttaatgaacg aatttgtaca agtatttccc gaaaattggc
    22981 agaaaaaata tgaggataaa gggtttatct caccttctct tattcaacga gcagtttttc
    23041 agccactaag taataaacaa agtatagtag cggtttctcc tacaggttca ggtaaaacac
    23101 tggcttatct ttggcctcta ctactaaacg tggaaccagg tgaagcaagt gcgttggtca
    23161 tttttgcttc ttctcaagaa ctagctattc aagtagcaga tgttgcacgt gaatggggaa
    23221 aagacaaaga gttgaaagtc caatctttgg tgggcggagc caacgtcaaa cgtcagattg
    23281 agggtttgaa ggaaaaacca gaaatcttga ttggtacacc aggaagaata ttggaattga
    23341 tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat ggtatttgat gaagcagacc
    23401 agctatttga cgctgggaac agccaaatca ttgatcaaat tcttcatcaa gctccaacag
    23461 agtatcagct agcttttttt tcagcgacag cagatcgttc ccttgaaagc attgaaaaaa
    23521 tcactgggaa aacgtttgag acaattgacg tgacagcaga ggacgattct cgaaaaggac
    23581 tgagacatta ctttttacgt gtaccgatac gaaagaaaga tgactacttg agacgtctca
    23641 cgcatataga agattttcat ggtctgattt tctttaatca actgactgaa ttaggcgtaa
    23701 tggaagaaaa attgctgtat agaggtgtac cggtaggaag tctggcctct gatcagaaca
    23761 agctactccg aaaagcagcg ctagaacaat tcaaaaagaa aatggtcaga gcacttttga
    23821 ccacagatgt ggcggctaga ggtttagata ttacaggtct tccttatgta gtgaatgcag
    23881 aagttccgtt aagtgaagaa gcttatttgc atcgttcggg aagaactggt cgaatgggta
    23941 atgaaggaat agtaattaca ctcgtacaag ataataatct aagagattta aaacggcttt
    24001 tacgtccatc aaatataaca cttgaagaga tttttttata cggaggtaag ctgcaaactg
    24061 agcagccaac caaagaagat gcagctcctg cagtaaataa aaagtatgcg aaaacttttt
    24121 ctggaaagga gcagaaccca gaatataaca aagaaaaaat aagaaaaaaa gttaaaaata
    24181 aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata agcgcctttt tatgctatga
    24241 ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt tatgtgaaag aacgtcaaga
    24301 agagatcttg cagctattga cagaaaacca acaaggattg acagcaagtg atgtagctga
    24361 gcgcttaact atcgatcgaa gcaatgcaag ccgatattta agtgaactat acaaggccca
    24421 tcatattgta aaaaccgctg gccgaccagt cgtttactct ttgccaacgg aaaagtcaaa
    24481 atctgatgaa gtgcatgtcg actcttctac gcaagtgact tttgaaacac tcgttggaga
    24541 aaatgattct ttaaaagtca gcatacagca ggcaaaagct gcgattcttt atccaccgcg
    24601 ggggttgcat acgattattt ttggagaaac tggtacaggg aagtcgatgt ttgctgaatg
    24661 tatgtatcat tttgcaatcg attcagagat gctttctgca gatgcgccat ttgtttcttt
    24721 caattgtgcg gattatgctc aaaatccgca actgctcttt ggacacatct ttgggatcaa
    24781 aaaaggggct tacacaggag cagctcaaga tagtcccggt ctaatagcca aagcagacgg
    24841 cggtatattg tttttagatg agatccaccg tttaccgccg gaagggcagg aaatgctgtt
    24901 tactttcatt gataaaggta tttaccgtcc gcttggagaa agtgcacaag tacatgaagc
    24961 atctgttcag atcattgggg cgacgactga atcttcagaa agtttcttat ctacgttcaa
    25021 tcgacggatt cctatggcaa tcaccttgcc gaatcttgct tcacgttcac tggatgagcg
    25081 ttatgaaatc atctctcttt ttatcaaaca agaagcgaat cgtttgaatc aacgtatcga
    25141 tatcgaaaaa gaagcgattc ttgccttcat gctttatgat gcggaaggaa atatcggtca
    25201 agtgaaacga gacttgaaac tagtctgtgc aaaatctttt ttacattacc gtactcatca
    25261 tgagaaaaag ctaatcattc gaaaagaaga actttccctt caagtacaaa aaggattact
    25321 gaaaatcaag gaaataccgg aacgtctcga ccgattcatg gacagcaaaa gtcagtattt
    25381 gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa gatccagaac gaaacatgca
    25441 ggtctataac gatattgagg aaaaagtcac ttctctttcc gaaaccggtg tggagaacat
    25501 tgatctagag accctcattt caaaagatgt ggatgcttat ttccagacat atgtcaaaga
    25561 attgactaaa agttcgattc ccaaagatct tttgccagat gaaatctggc ggttaaccaa
    25621 tcaattgtat gatgtggctg aaaaacggtt agaccgtatt tataatgaaa aagctcgttt
    25681 tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg agagaaggtc atatgatcgt
    25741 ccacccagat ttgaataacg tacggaaaaa ttttaagagg gaattccagg tagcaatcga
    25801 tctttcaacg atcattgaag aagaagaaca agttgagatt ccttttgatg aaatcggctt
    25861 tattagtatg tttctttcta tcaatctagg agaaagtgag cctgttaaag aaaacaaagt
    25921 agatgttctc atattgatgc acggcagaca gactgcttca agcatgttgg agatggcaca
    25981 agaactatta ggaacaaaag tagggaaagc ttttaatatg ccactggtta tggaagttca
    26041 aacaatgtat gaagaagtgt tacaatatgt caaaaactat caagagcatt tgacgaatgg
    26101 cctactcttg ttaacagata tgggatcatt aaacaccttc agtgatttga tttatgaaga
    26161 gactggcatt cggacgaaag ctatcacgat gacaagtacc atgattgtga tagaagcgat
    26221 acggatggca agtgttggaa gaagcttaga agatatttat caaaatatcc agctttcctt
    26281 tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct gaaactttac agcgtaaaaa
    26341 agccgtaatc gtgacatgct ttactggtga aggagtagct gccaaattat atcagcgtat
    26401 tatgccagtg attaatcaaa ataaggtaga actgatccag atgcagtttt tagaacgtga
    26461 atcattcaaa aaacacatcg atgccttatt ggaagaatat gagatcaaag caattgcggg
    26521 aacagttgat gttgattatc aaaatatccc atttttttca gcctatgacg tttttgatga
    26581 tgaaaaacta aatattttaa agcgtatagc tgatgatgag atcccgacag ctaaaattgt
    26641 cgattcactt gctggatcat taagcgatgt cttttctatt gatgaactga tccgttcatt
    26701 gcaaaagaca gttcagcaga tccagaccga tttgtcgatt atcgtggaac ctggagtaga
    26761 tactggaatc gttatccatt tagccttcat gttagacgga ttgatcaaac aagaaagcca
    26821 tcggcaattt gagaaactgt ctattttctc aaaaatttat cgtttggaaa tggatatcgt
    26881 tcgggcaaac ttgatggcac tagaacgaaa atatcaggta aaaattccag aagatgatat
    26941 cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa aaatgaacac actaatcata
    27001 ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa gagctaaaaa gcatgaaaac
    27061 gctatttcag ctcttttttt tattggcatg cttcttgcgt tatataaaag tgtaaaaaaa
    27121 taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt agtttgttca gcagggatga
    27181 gtacgagttt attggtcaca aaaatgcaaa aagcagcaga agaaaaaggg atggaagccg
    27241 atatttttgc ggtctctgcc tcagatgcag ataataattt agaatctaag aatgtcgatg
    27301 tattattatt aggtcctcaa gtacgtttca tgaaagctca atttgaacaa aaacttgcgc
    27361 ctaaaggtat tccattagat gtcatcaata tgcaagacta tggcatgatg aacggagaaa
    27421 aagttcttgc acaagcagaa aatctaatga aataaatcaa gtatgcagtt gggggtataa
    27481 caacgtggaa gatcaacaaa atctagaagc catcatgggg ttaatcatgt ttggcggtaa
    27541 tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa atcggtgatt ttgagttagc
    27601 ggataggaaa atcgctgatg cagaagaatc acttatacaa gcgcatcatg cacaaacaga
    27661 catgttgaca caagaagcac aaggaaatca tatgcaagtg acattattga ctgttcacag
    27721 tcaagatcac ttaatgacat ctattgcgtt tactgattta gcaaaagaaa tcatcgaact
    27781 ttatcgtcgc gtatacagcg aataaataca acttcttttt tgaacggaag aagaaaaatt
    27841 tttgaacgga ttttttattt tgttataagg agaactctct ttatataaaa aacttatagg
    27901 aggaagtaaa aaatgaacgg actatcaagt tggatggaga aatacatcct gccggttgcc
    27961 gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg atgcttttat cggcacactg
    28021 cccgcaacaa tggcgggatc agtcgctgta atgatcaatg caattattcg tgatttaccg
    28081 caacagttta acagtaccta tgctgacagt ttagcagcag acaaaggaat ctttgccgta
    28141 ttgaatgtca tcatcggaat caatggtttt gtctggaacg gtacactagc aatcgccgga
    28201 ttgatctttg ccttttcttg ggggtataat cttgcccgag cttatggagt aaatgatcta
    28261 gctgggggga tcgttggttt agctaccttg attcaaggta tttcctttgg tccagcatta
    28321 tcagcagcat tagatgtgaa tgttccttca aatgtcgcag atgcaatcaa tagttctgag
    28381 attggtgcaa cgattgcaga tggcacactg tcagttggat tatggggttg gctaaaacta
    28441 gatcatttga atggaaacgc ttattttaca gttatgatca tgggagctat ttcagtcatt
    28501 attttttgta aattgatgct tgccaacatt acgatcaaaa tgcctgattc agtaccacca
    28561 gcagtatcta aagcatttgc agccatttta ccagcgacga tcgctttata cgtcattgct
    28621 attatcaatt atatcgtagg tcgtgtagct gatgggcaac tgatcattga tttggtgcaa
    28681 aaatatattg cagaaccatt cctaggatta tctcaagggt tgggagcagt attgatcgtc
    28741 acagtatttg ttcaagtatt ctggttcttt ggtattcatg gatcaaatgt attggcacca
    28801 gtgctagagg gaatctgggg acaagcacaa ctaatcaata ttgatatttt ccaaaaaggt
    28861 tataacggaa aaactggtac accagccgtg ttagatgcaa tcgaagatgg aaaagcttat
    28921 atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg gcggatcagg cggaacgatc
    28981 gtcttactga tagctatctt gattttctct aaacgtgcag attatctaac agtagctaaa
    29041 ttatccattg ggcctggtat cttcaatatc aacgaaccag tgatgtttgg tctgccaatc
    29101 gtattgaatg caatcatgtt tatcccattc ttgattgctc cagtagtagc aacagcaatt
    29161 ggatggacag ctacttatct tggcctagtc gctccggtat cgcagcaagt cacatgggta
    29221 gtaccgccgt tcttattgtc attcttagca acaggagcgg attggcgggc accgatcgta
    29281 acattagtat gtatggtcgt gactttcttg atttgggcac catttgtcat ctctgcaaac
    29341 aaaatggacc ctgccgcagg cgaacaacaa taagatgaac gtgaggagcg ggacgcttta
    29401 agtttcgttc ctcataatga attttcgagg aggaactgaa atgattacaa ttgccagtgc
    29461 aatgcaacaa gatgaaatca aagaattgtt agaaggatac aatgaagaag gaatgacttt
    29521 tacatttaaa gaaaaacaag gaatcaagct gttctttgaa acaagtgcag aagatccgga
    29581 agttgcggct aaaaaagcaa aggaactaat caaagcacag ccttggggaa cagtccttta
    29641 ttttcaatca acagctgttt agaaaatggt ttaaaaagat aggatggttg agatgataaa
    29701 aaacggttta tttttatgta gtattggttc tttgatcgtg ttgtatagtt tgaatccaca
    29761 ttcgcaaagg tatgatttgt atagtatggt cacaggtctt tttctaacag gattaggaat
    29821 ctatttcttc atgaagggga aaaaaagaga agaagcaaag gagaaaaata actaatggaa
    29881 atcacgaaaa aattaaatat tccagcatcg tttttttacg ataaagttat ggactctgtc
    29941 cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta gaaaacagct gaaaaatttg
    30001 gagtacgtca aacaattctc gcaaaataac cgggctcgca taaaaatcga agaagtcatt
    30061 gaaaaccagt cttataagtt tcgtacatcg acaacgaaga atgaattcgt cgttcattat
    30121 caaatcaaac cattggacga ttcgacctgt gaggttcatt acacagaaaa aatggagtct
    30181 tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa caatcttgat gtattttaaa
    30241 cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa cgtattaaat caatgaaaca
    30301 tggtactatt tagttattga aaaatgaagt aagctagaag gaatttttca taggaagata
    30361 gatctttcct gcgaatcctc actagctttt ttgtaggaag gggcagcaaa atgacaaaca
    30421 aagtaagaga gctaggtgta tcttcgattg aggaaatgtt tgatttagcc acttatgcat
    30481 ttaatggggc agatacacct gaaaggcgag aacgttttcg tacacttgcg gagaattctt
    30541 ggaattacgg attttttgat gaggaggatc ggctaacaag tcaagtcatg gcgacccctt
    30601 ttccagtgaa tttttacgga caagagtacc taatggccgg cattggctat gttgcttctt
    30661 atccagaagc aaggggacaa ggcgggatca atcagatcat ggaaaagatc cttgaagatt
    30721 gccgtgatcg taaagtaagc ttatcttatc tggcgccatt ctcctatccc ttctatcggc
    30781 gctatggcta tgaacaatca tttgataaaa tcagttatca actaaacagt cgtgatgtgc
    30841 cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga ttttgaagat gcaaaagctg
    30901 caatcagaag aatttatgaa cagatgccgg aaaaccaacg agctggtctg aagcgcgaag
    30961 actggtggta tacgtataaa ttcaagcaaa aaggcaatta ccagtttgct ttgcattttg
    31021 atgataatga ggaagcaacg ggatatatcg tttatcagct tgcaacgtct tcctttatta
    31081 ttgcggagtg gggatttttg gatcatgatg ctttttgcag ccttgttcgc ttcgtaagtt
    31141 ctcacaatgg tgcttttgaa acatttgaat attcatgcgg ctacggcggt aatgatcaaa
    31201 attatctatt agaaaatcca tttgcttctg taatgatcac accttatatg atggcgagga
    31261 tcgtagatat ttctttgttt ttggaaaatt atccatttaa aaagagacaa gctgcatttg
    31321 ccctacaaat agaagaagat cattatgcga aatggaatga aggaatcgtg gaagtaacaa
    31381 tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac aaaactccca atcgttcgag
    31441 gaagtatcca acatatcacg cagctattat tagggtatcg taaagtggaa gagttggtat
    31501 tccaagaaag aatacaggtg acaaaagaat atgaagaaac gttaagctca atcttgccac
    31561 agcaacagcc aattcttaat gactattttt gataaaaaac gggtgatgac aatatttttg
    31621 tcaccaccct tttgtttttc atttggcatt tgaaggaaaa tgttaaagta aatcctcttt
    31681 tttcgtaaat ttgaattttt tgttttcttt tgttttaggg ttttcttttt tgtggtcttg
    31741 gattgcttct ttcgtaatct tttcccgatt ttttttagat gaatctaagc gttctgcgta
    31801 tactttttct tttggcttat cattcattgg aaaggacctc cctacattat ttcattctac
    31861 ctctagttta tcatctgtta aggactagtg gaaacgtttt gcctaactta taagctgatt
    31921 tttttagcag ataagcttgc aagtaaaaaa caaacaagct acaattgatt tattgaaatt
    31981 gttcctgtag ctcagtagga tagagcaatc gcctcctaag cgataggtcg ctggttcaat
    32041 tccagtcagg aacgtataaa taacccctgt atcctttgcg gtacaggggt ttgttttgta
    32101 tttgtgccaa aaatgtgcca aactagttat catttttata taaactatcg aacacagtat
    32161 tgattttttc tttatcctga tcttcaagct ctcgaacaat atgaagataa gtagacattg
    32221 tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt actccttgat
    32281 aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa agtaaattgc ttttcaagcc
    32341 ctaattcttt taattttttg cgcaaatatt tgctgacggc gtttgatgaa actaatccat
    32401 tttttaagtt gaaaaaaaca aaattatgag ggtttttgat ttcaaaattc tcgaataatt
    32461 ctttttgatt gattttaaac ttttttaaga gatcaatcgt atgtttatca atggaaattt
    32521 tcctatttga agtttcgttt tttgtttttc cccattcatt taatttataa ttccatgttt
    32581 tattaatagt gatgatttga tcttcgaaat cgatatcttc ccatgttaat cctagaagtt
    32641 cagcaaatcg cattccagtg gcaccagcaa ctaacacgag cataggagaa gagtactgcg
    32701 cagaaaggtt ttcttctacg agtttcatta aagatttaaa ttgatcataa tctaaatatt
    32761 tatcttcttt tggcttcaat gaatcttttc ctttgattac tgcttttcta gttggatcaa
    32821 acggaattaa tccttcttct acggcatctt tcaacgacgc tctaatatgg ttattaaact
    32881 taataacggt agattttaca tggtctttag catacttatt taaaaagcgt tgatatccca
    32941 ctctatctaa atcagaaatc aataccgctg gcatatattt ctttatgttc attaacgtat
    33001 cttcgtattt tctataagtg ataggagata ctgttccttc tttataaagt tgcatccaat
    33061 cctcaaagta atctgataat aatagatttt tgcgatcccc tttaaggcct ttgttcaagt
    33121 tatattctaa ttcgttggcg gcatcttttg cttctccttt tgtcttaaat cctgattttc
    33181 ttatcttcgt gtattttccg tcatcttttt tgtatgaaat ttcgtattgc caactatttc
    33241 cacgtttgac taatcttgcc ataattgaat cagctctctt tctctgatac aataggaact
    33301 ataaagaagc ccattgtttg ggttttatta ttttttgcac atcctttttg cttgccggcg
    33361 caaggatgtg ctatttgtcg ttttaatggt ataattggat gtgtattaga tcatccaatc
    33421 agaggtgttt attatgatta aagtgatttt taaaagaata actgaacttc cagagtttag
    33481 attatttgtt gattattggt atgtctggtt ttttattgtt gcattgctga taggtctaga
    33541 tgtcatatta gaaagaaaac ttaagtaaga gttgagttaa taaattgaat tcctaaatga
    33601 gatattttgt agcccatatt ttgtttgccg acatcaccta atactatgga gctaatcgtt
    33661 ttttctaaca gaccaaaatc agataatcta tttaagctac catctattag ctcttgtgaa
    33721 tagccagaca tgctgatatt tccagtgaaa ttttcgtttc cacgactcat taagcatgct
    33781 aacaattgaa tctcaatcga tgttaattga ttcagtacaa gaagataata ctcctcgtta
    33841 tcccatgaag cgttgttaat tcgaagtaaa caattcttat aaagattttt atacatatct
    33901 attttatttt gtgcctttga tttttctacc tcgtcattta tcccttcgaa tattccaatg
    33961 agttggtcct tgttctctaa gttgaaaaca tcttctggaa tttgatcgcg aatgttttct
    34021 aatgattcgt ttaaattttt gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta
    34081 gacccaaaat aaagtgactg cacaacgcca ccaatataag gaattgctcc aataccgcct
    34141 tcaactatta attcaagata ttcttttttg gttagttttt catttctatt atccatagtt
    34201 acctcctatt ttgtcatttc ctttacaagc attgtgactc ctgatggcac tcttttgtat
    34261 aggttttatt attgtgcaca atctaactct tagtggggcg tggttcattt attttttact
    34321 tactttacta ttaatcatat cccattgagc ataggatttt tctatgttcg atcctcttga
    34381 actaatttct cctgtaaaag ttactttatc gccctttgta aaggtatcag cattgtttaa
    34441 gtccttcgca aaaattacat atgaaacttt aggtgttgca gaaatatcat tccaactttt
    34501 atcatcgtac tctttgtctg ctataatggc tattctagta gtcattgatt cgataacagt
    34561 tccttcaaaa gtatatgttt taccactaat atatttagta aaagctgtat tttgctcagt
    34621 agctggagta atagaataat aactatctaa gaaagcaccc cagtcatgat tatttgtctc
    34681 taaaaattct tcaatttgag ttaggctttt attgctgctg ttagatgtag tagtttgagt
    34741 atcagatgat gagctttcct gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga
    34801 gaaacctacg gtagtaaaaa taatcccgat aataaggaga atcataggtg ttttcttatt
    34861 tgtttttttg ataaatctta caattaacat gattaaacct ataataaata aaacaatacc
    34921 agtaaaacca aatataccgt ccattaatag atcctctttc tcttttgata ttgaatatta
    34981 taaaccaagc aattcttttt tcttaaaatc gaattcttct tgattaataa tcccttcatc
    35041 taacaactct ttatactttt taatttcatc tgcacttgaa atttgtgttt ctttggaatt
    35101 attatctaat atagattgtt gcttctctat atattttttt aaattttcca tttcattcca
    35161 atattttttt gtaaacataa cggtgttttc atctttcgtg gccgctaata tacctccgcg
    35221 actttcgttt ccgcctagca aggtaaattg tatataacca ttactcatac ctggtttttt
    35281 taattgtacg gctgatatat ttttaaaggg gatagttttt tctcctttaa taccttgatt
    35341 aactaaattt ataaatcctt ttctaataat tgaaatagaa tcatcagtta ctttaatcaa
    35401 ggtttttcct ggagatttta ttaaaatctc tttttccatt ttttcttcct catttctgtg
    35461 atatgatttt ttttgtagaa gctcatatat gaggaaagag cccgtgttgc agcacgggct
    35521 tttttaatgt tttgctgaaa tcggtttttt taataattct tgatgtattc atacatattg
    35581 ccttgcgtaa gaatattttt tc
//
LOCUS       DAZKCZ010000017        35586 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000017 DAZKCZ010000000
VERSION     DAZKCZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.017"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..533)
                     /locus_tag="ACG8KN_000712"
     CDS             complement(207..533)
                     /locus_tag="ACG8KN_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187794.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(545..745)
                     /locus_tag="ACG8KN_000713"
     CDS             complement(545..745)
                     /locus_tag="ACG8KN_000713"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9187795.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            868..1470
                     /locus_tag="ACG8KN_000714"
     CDS             868..1470
                     /locus_tag="ACG8KN_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9187796.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1525..2691
                     /locus_tag="ACG8KN_000715"
     CDS             1525..2691
                     /locus_tag="ACG8KN_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9187797.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(2780..3172)
                     /gene="rpsI"
                     /locus_tag="ACG8KN_000716"
     CDS             complement(2780..3172)
                     /gene="rpsI"
                     /locus_tag="ACG8KN_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9187798.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3188..3616)
                     /gene="rplM"
                     /locus_tag="ACG8KN_000717"
     CDS             complement(3188..3616)
                     /gene="rplM"
                     /locus_tag="ACG8KN_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9187799.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(4150..4617)
                     /locus_tag="ACG8KN_000718"
     CDS             complement(4150..4617)
                     /locus_tag="ACG8KN_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFP9187800.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4737..6275)
                     /locus_tag="ACG8KN_000719"
     CDS             complement(4737..6275)
                     /locus_tag="ACG8KN_000719"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9187801.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6355..7041
                     /locus_tag="ACG8KN_000720"
     CDS             6355..7041
                     /locus_tag="ACG8KN_000720"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9187802.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7242..7769)
                     /gene="msrA"
                     /locus_tag="ACG8KN_000721"
     CDS             complement(7242..7769)
                     /gene="msrA"
                     /locus_tag="ACG8KN_000721"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9187803.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7853..8530)
                     /gene="pgmB"
                     /locus_tag="ACG8KN_000722"
     CDS             complement(7853..8530)
                     /gene="pgmB"
                     /locus_tag="ACG8KN_000722"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9187804.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8527..9657)
                     /locus_tag="ACG8KN_000723"
     CDS             complement(8527..9657)
                     /locus_tag="ACG8KN_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9187805.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9657..11840)
                     /locus_tag="ACG8KN_000724"
     CDS             complement(9657..11840)
                     /locus_tag="ACG8KN_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFP9187806.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11837..12862)
                     /locus_tag="ACG8KN_000725"
     CDS             complement(11837..12862)
                     /locus_tag="ACG8KN_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9187807.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12907..13734)
                     /locus_tag="ACG8KN_000726"
     CDS             complement(12907..13734)
                     /locus_tag="ACG8KN_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9187808.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13731..14798)
                     /locus_tag="ACG8KN_000727"
     CDS             complement(13731..14798)
                     /locus_tag="ACG8KN_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9187809.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14824..15660)
                     /locus_tag="ACG8KN_000728"
     CDS             complement(14824..15660)
                     /locus_tag="ACG8KN_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9187810.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15657..16526)
                     /locus_tag="ACG8KN_000729"
     CDS             complement(15657..16526)
                     /locus_tag="ACG8KN_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9187811.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16596..17879)
                     /locus_tag="ACG8KN_000730"
     CDS             complement(16596..17879)
                     /locus_tag="ACG8KN_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9187812.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17893..19578)
                     /locus_tag="ACG8KN_000731"
     CDS             complement(17893..19578)
                     /locus_tag="ACG8KN_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP9187813.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19853..20878
                     /locus_tag="ACG8KN_000732"
     CDS             19853..20878
                     /locus_tag="ACG8KN_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9187814.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21473..25126)
                     /gene="rpoC"
                     /locus_tag="ACG8KN_000733"
     CDS             complement(21473..25126)
                     /gene="rpoC"
                     /locus_tag="ACG8KN_000733"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9187815.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(25205..28822)
                     /gene="rpoB"
                     /locus_tag="ACG8KN_000734"
     CDS             complement(25205..28822)
                     /gene="rpoB"
                     /locus_tag="ACG8KN_000734"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9187816.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29550..30518
                     /locus_tag="ACG8KN_000735"
     CDS             29550..30518
                     /locus_tag="ACG8KN_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9187817.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30536..31441
                     /locus_tag="ACG8KN_000736"
     CDS             30536..31441
                     /locus_tag="ACG8KN_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9187818.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31438..32244
                     /locus_tag="ACG8KN_000737"
     CDS             31438..32244
                     /locus_tag="ACG8KN_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187819.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32401..33369
                     /locus_tag="ACG8KN_000738"
     CDS             32401..33369
                     /locus_tag="ACG8KN_000738"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFP9187820.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33686..34210)
                     /locus_tag="ACG8KN_000739"
     CDS             complement(33686..34210)
                     /locus_tag="ACG8KN_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFP9187821.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34497..>35586
                     /gene="scm"
                     /locus_tag="ACG8KN_000740"
     CDS             34497..>35586
                     /gene="scm"
                     /locus_tag="ACG8KN_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP9187822.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDT"
BASE COUNT        10180 a         7544 c         6588 g        11274 t
ORIGIN      
        1 agaattagaa tagccttgct tttcaactaa gctttcaact tctttttcat tcattgtcac
       61 acctgcttct gattttattt gctataatag attcaaataa agttttctca gaactttatt
      121 attgagtgtt cccccgatct gcaaactgac gggaacactc tttttaattt tttctacttc
      181 atcaaattta aaaaacgagg tgttttttaa atttgatgac tcaatatcca ttttttgcat
      241 tcttcttttg aaaacaactc cattttttct aataaaataa ctggcaatcc cattctttta
      301 aatttcaaaa aagttgtata tgatacattc aaatatttag ccatctcttt tccttttaga
      361 aatggtcttt ctaatccggt atcaattctt gcttgatcaa tagcctcttt tgtcatttca
      421 aaaacataat ttttcaattg attcgtttgt tgtttatcta acattactgt aattcctgca
      481 tccattattt ttctcctctc attttctatt tcttcaatca gccaactcgc caagttgtat
      541 ttatttagat tttcaaatca ttaagtaacc aatttaccaa cttttgataa gtagatgctc
      601 taatactttc tttgctccca ttttcgattt tgacatatgt tttccgattt atacctatca
      661 attttgctgc atcgttttta gtaagtataa gatcggctct atgtcttctt aagcttctaa
      721 tgaattgatc attaagagtt atcattcagc gcctcctttc aaattattca ctttgtggtt
      781 tttatcttaa taagcacatt ttaaaccaca aaacgagtaa tatcaattaa aatattactc
      841 aatttgtggt tttcatgcta taatagtttg gaatttacta tagggggcgt tactttgaat
      901 agaataaaat tttatattga ttccagtaaa aagaccatta aacaaattag tacagaaact
      961 ggtattaact atacaactct tacgaactat taccgtggtg agcgaaatcc tagaaatact
     1021 gaaacatgga atatccttgc tgattatttc catgttcctg taagttattt gatgggatta
     1081 gacgaaacaa attccataga acaacctaat cttgatagaa taaaaactgc tcgtttagaa
     1141 aaaaaggaca aaatagtcaa gaatattgcc agtcttgact cagattattt tactgaatta
     1201 tctaatctat acttttccat gacttcttta cttgaagtca aacttcaaga agatgactcc
     1261 gactttcttt ttttaaaaag tattctagag gactcctttg aatacacgat tctatttgcc
     1321 aaacaaaaga agtttattaa taaagcttta aacttaaata tcgatgaaag aaaagagtta
     1381 gaatctgagt tatttggcaa cttaacccaa gtacaggtta acaatctaaa aagagagata
     1441 acagctcgat taatatcaaa acttcaataa aactaattat tcaatcagcc aactcgccaa
     1501 gttatcagat tggagagatt agttatgtca atacaaaagt acacaacaaa aagcggtgca
     1561 cgatatagtg tccgcttgta tgttcgtaaa gatgaaaatg gaaagcaaga atactatgta
     1621 aaaaaagggt ttaaaactga aaaagaagcc aagttacacg aagcaagaaa aaaggtcgag
     1681 attgaagata ccggctatca taagccttct aatgacttgt ttataacagt gtatgaagaa
     1741 tggttgcctt cttataggaa tcgtgtacaa gaaacgacat atcaacgtac aaaagacctt
     1801 ttccgcttgc atatactacc aaaattcggg aataagtcca tttctaagat tactcctgtt
     1861 cactgtcaga atgcagtcaa tgaatgggct ttaaaattca agaatatcaa acagcttaag
     1921 tcttacagct ctcaaatatt tgagtatgcc atttttgcag agctattaca ccgaaatcct
     1981 atggcaaata caaatctacc aaagcgggaa aagaaagaaa atgaaaatta cttttcatta
     2041 gaagaactaa aagctttttg ggatattctc aataaagaag agcctttaaa acatatttta
     2101 atcttccagc ttttgattac tactggtata cgtaaaggag agctagctgc tcttcattgg
     2161 tctgacattt attttgaaga acagctaatg tatgttagaa agagctatgc aactattcga
     2221 aatgaagaga agaatgcaag aagaaaaact gtcaggattc aaaaaaatac aaaaaatata
     2281 tcttccgaac gaatattacc aattgatgca caaactattg aaatgttaaa aatctggaaa
     2341 aaagaacaag ctcgagagct tttgcagttt ggcataaata caaatacaaa aaatcaactt
     2401 atctttacct atgtaaatgc agacggtgac attaatcaac ctttgcatgc ggactattca
     2461 aataatatat tgaaacgttt agagaagaaa tataaattta agcacgttac tattcatgga
     2521 ttccggcaca ctcacgctac tcttctacta gaaggtggag caagtatcaa agaaacacaa
     2581 gatcgattgg gtcataaaaa tgctgaaact acccttaaca cttattctca tgttactgaa
     2641 aaagctcaac gaaatgcgtt agaaaaattc attgcttaca ctggtttata atattgtcat
     2701 tacctttctc attaccagaa cgaaaaaaag acaccttctc gcaatgagaa agtgtctgaa
     2761 atcgttggta taacaacgat taacgttttg aaaactgtga agctttacgt gctttcttaa
     2821 gacctggttt tttacgttcg accatacgtg cgtcacgagt aagtaagcca gcacgtttaa
     2881 gagctaaacg gaagtcagga tcaacttcta gcaatgcacg agcgatacca tgacggatag
     2941 ctcctgattg tccagcgtat cctccgccgt ttacgttaac aaatacgtca tatgcgcctt
     3001 ttgtttcagt aacagcgaaa ggttgattga ttacttcacg caagtcagca tgtggaatgt
     3061 attcttcaac atctttttta tttacagtaa ttttgccagt tcctggtact agacgtacgc
     3121 gagcaactgc gtttttacga cggcctgtgc cgatatattg aacttgtgcc aatgaaattc
     3181 cctcctatta gattaagttt gtgatgtcta aaacttctgg ttgttgtgct gcatgtggat
     3241 gttctgctcc accgtatacg ttcaattttg taaattgttt gcggcctaaa gtgtttttag
     3301 gaagcatacc ttttacagaa gtttcgatca aacgacgaga atttttagca cgcaattcgc
     3361 cagcagtgat tgatttcaat cctcctgggt agttgctgtg acggtaataa attttgtcag
     3421 tcgctttttt acctgttaat ttcactttgt ctgcattgat gacgatgacg aaatcgccag
     3481 tatccaaatg aggtgtgaaa gttggtttat ttttaccacg tagtacagat gcaacaactg
     3541 ttgaaagacg tcccaatgga acatcagttg cgtctactac ataccattta cgttctactt
     3601 cgcctggttt ggccatatat gttgtacgca cgttattttc ctccaattta agttctgatg
     3661 tttgacatac acttcgagtt tccggggctc tttgtggggc aaacaatacc atttaacata
     3721 ttacccgcta atctagtcaa tgtcaacgtt tttctgcttg attgaaaaat ttatatgaaa
     3781 ctttacttct tttctttctt ttatataaaa gggtcgtttc ctgtcaaaat gaaaaacaaa
     3841 aagaggctgt gacaaaagtc ttgtcacaac cccttatttt gaataaacgg tgttctcctg
     3901 ccaactcctc ggtcacaagt cacaatatcc ataaaacata agaagctgtt ttttaaaatt
     3961 gtttcttgtg actcggagct gaacggcagg cttacaacct cttggtaaac ggtgttcaat
     4021 cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgacgttt
     4081 gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctttttcgg tcaagttcaa
     4141 caacagtttt tacgcgtcga tctctggcgc tttgcccaaa tgagcttgga tcatccagat
     4201 acgtttttct gtggctgttt tgaattcgat aaagatatct tgtgttggat cgtcgccttc
     4261 ttctccagaa acttcgattc ctttttggta aagatctgct agataacgat agcctgcaac
     4321 aagtgtttcc aaacgttctg gaatagtacg atcccagcgg ccaatttcat cagggatttt
     4381 tgtgttatct gcaaattcac gcaatgtaga ataaggtgaa ccgtctaatg tgatcaagcg
     4441 ttcggatact tcatctaact gagcgttcaa atcatccatg aactcatcca tcattgggtg
     4501 caatgtaagg aaacctggtc ctctcatgta ccaatgtgtt tggtgaacaa caacagacat
     4561 ttggcttaga tctgcaacta attgatttaa tacttctttg gtttgttgga atttcatctt
     4621 tcttgcctcc taacattttc ttacacttaa aataatagta gactttcaat gaaaagtaca
     4681 acaaaatcat ttacctgaga acgattttca gttcgctttt ttatccgtaa agccaatcat
     4741 ttttcagaat cagctaattc ttctcgcagt tgagcaaatt gctgataagt ctgtgctaaa
     4801 tgcttttgtg tcgaaacaaa taatggatag attttctgat attttttatg atcagtttct
     4861 tgtactccaa cttgctcttt caattctatc aattcctgtg cttcactgat actttggatc
     4921 tttccgatgc ttctccagcc taaaatcaca gctcctaatg cggatgcttc tgtgacagta
     4981 gggaaaacaa gcgggtaacc cagtacatca gccatcatct gacgaaaaat cggagattga
     5041 gcaaacccgc ctgtagcacg gatctctgtg agctgatttt cctggcaaac tccttttaaa
     5101 atccgataaa ggttgagaca gatcccttct agaatcgcct tcatcatttc tttttctgta
     5161 tgttgccgct gtaaaccgat aaaactgcca aacgcatctg cgtcccaaag cggtgctcgc
     5221 tcaccaagta aatagggatg aaaaatcaga ccattggcac ccgctggcac aaaagcgata
     5281 gccgaaagca gtgcatcata tggattttct cctttttctt tagcagattg aacgacttcc
     5341 tgtaaaaatg tatgccctgc ccaatcaaag atgccagcac cattgctagt cgctcctccc
     5401 actacccaat gtttctgatc cagaacataa caaaacgtct ccatttctgg atggaactga
     5461 ggctgagtag taataaaacg aagtgctccg ctcgttccta cggtcaaagc ggcgatcgta
     5521 tcaccaaaag cacctaaccc tagattagaa agcggcccgt ctgcccctcc caaaacaaat
     5581 gatatatcat ttggcaaacc tagttctgct gctgtttttg atggtagacc tgttacttga
     5641 taggtggaag gaaagacttg cggcaattgc tctattcgaa tgttcaagta gtcaagcact
     5701 tcttctgccc attggaattc atggatatca aaatatccag ttcctgaagc tgttccgtag
     5761 tcgctgatca gttcgccaaa caatctataa aagatatagt ctttgatacc gacaaaataa
     5821 gcagtttttt tgaataattc aggttggctg ttttgcagcc acttgatttt gttcaatggt
     5881 gtcattggat gaatcggcgt cccagtctgc cgataaaatt ctttgccaag cacggtatat
     5941 ttcagttttt ctgcttcgtc cgctgctcgg ttatctgccc aagtgatcat tcgagataac
     6001 ggtgttttct cttcatccaa caatatcagg ccatgcatcg cagaggagaa ggagacaaat
     6061 tgaatgattg tgtctgaagg caactgttta attacttctt ttataacggt gcagacacct
     6121 tgaaagatca attcctgatc ttgttctgcc atcccttgtg tttcttggat caaagggtag
     6181 cgaaaataag cggatgctgc ttcttgggct tcatctgtga aagcaactgc tttggtttgt
     6241 gtggttccga tatctaatcc gattgaaacg ttcatttttt cacctcaaat tttatgtatg
     6301 tacaaaaatt tagtttaagc ttgcgtatct tggttttgaa aagggggaga tggcgtgtta
     6361 ctttatttta tcattggttg ctgtatcgga tcatttttat gtttaaccgc ccagcggatt
     6421 ccgttaggtc actctattat ttatcctaga tctcattgtg tcaaatgctg tcgttcgctt
     6481 tcttggtatg aattgattcc gattctctcg atcatcgtcc aacgatttcg ctgccgctat
     6541 tgcaggtgtc ggttgccctt ctactatttg ttggcagaag ccttatgtgg tggtcttttt
     6601 gcctggttct ttactttttc ttccgcaaga aatttttcga cgttcatatg gatgctttcg
     6661 gctttgctat tttcgttgat ggatttattt tacttcatgg tccattctca cacgctattc
     6721 tgttcttggg caattttatg gattttttgg ttgcaggccg gcgtcttcca atggcaaagt
     6781 gtgctgttaa cgtttatcgt aggcggtgtg tgccttcgct atggtcaatc ctatctcgga
     6841 gctggcgata ctctgttgat gctttcttgg agtggtggct tgtctctaga agagttgctt
     6901 cagatcttgt ttcttgctag tctgtttgga atatccgttt tttctgttta ctttatctgt
     6961 cataaaaaaa agttggaaaa gctgcctttc attccttttt tgagcagtgc gttgctgatt
     7021 gtccttcatc ttaggtattg aaaaaaggtt gtgacaaacg acttgtcaca acctctgata
     7081 tatagtgttc tcttaccaaa tccttgtgac ttggagctag gcgacaggtt cacaacctct
     7141 ccactaaatg gtgttcaatc agctgaccct cagtattagc tcaaaatgct ggttttacaa
     7201 cctctgatat acggtgttcc ataagtagct ttttcggctt cttatttctt gacgccccaa
     7261 tgtcgacgaa ggaaacctgc acgtccagaa ccttctcgat aaagattgta atggagcttg
     7321 tttttcttgt aataatcctg atggtaatct tctgcaggat aaaattcgct tcttggttcg
     7381 atttttgtca cgattggctt atcgaatcgc ccgctgtctt gcaatgcttg ttttgaacgc
     7441 tctgcaatat ttcgttcttc ttcatcatta tagtagatca ctggacgata agaatcaccg
     7501 cgatcagcga actgtcctaa tgcgtcagtc ggatcggttt gctgccaata gatttccacc
     7561 aactgatcat aagaaataac ttctggatca tattcgatct ctactgcttc tgtatgccct
     7621 gttgtgcctg ttgttacttg ttcataagtt ggatttggta catgcccgcc agtataaccg
     7681 gatgtcacag aaatgatccc tggctgctct tcaaatggtt tgaccataca ccaaaagcat
     7741 ccgcctgcga aaactgcttt ttttgtcata taaaatcccc cttttttctc atcatacctt
     7801 gatttccaaa aggaggataa cgttttgctt gtgacagaaa aaacggaaac tattatatac
     7861 gtgatacggc ttctgataaa tgccaaaacg aacgtacttg atcaaaaacg gttaaatcag
     7921 gaatcacata ttcagcagaa ggaagattta tgttttcacc tattcccaat acatgcatgt
     7981 taccagccaa agctgcttga caaccagctt ctgaatcttc aatgaccaag catgcatttg
     8041 gcggtacatt caattgctgt gctcctttga gaaaaacttc tggatcaggt tttgcttttg
     8101 atacttgcgt gccgtcaata aggacatcaa aataacttgt caaattcgtt ttttctaaaa
     8161 tgaggcgtgc atttttgctt gctgatccca atccgatctt gatgtttgtt tttttcaagt
     8221 actcaagaac agctattgca ccaggcaaaa cggatgtttc atccattttt tgaatcgctt
     8281 gaagatatag attgtttttt tgctcagcaa aggctgattt ctctttttct gagaatttct
     8341 gttccatttt tccaaatgac agcaatcgct ctagtgactc ggttctggat attcctttca
     8401 gttgctcgtt ttctttctca gtgaatggaa tggacagctg gttagctaaa accagccacg
     8461 cttcgtaatg ataacgagct gtgtctacaa gaacgccatc taaatcgaat agtgctgctt
     8521 tgattttcat cgattacctc cttgaaaatg aacgacttga agtaaagatg cgatgttttt
     8581 agctgtgcga tgaagattat cagtggcttg ctgtaaagct tcttgtaaag gcacagctgc
     8641 aggaaggatc ggaaaaacgg ccgaaatgcc aacatctgct gctttttcgc ttcctttacc
     8701 aacacttccg caaatagcta tcacaggaac gccctctggt actcttctag caatcccaac
     8761 tggcgctttt ccttttagac tttgtccatc tagacgtcct tcgcctacga ttacgaggtc
     8821 tgcttgctgt acccgtttat cgaactccag taaatccaat acgtagtcga tgccagagac
     8881 tattttccct gaagcaaatg ctaacagtcc tgcacccatt ccaccaccag cactactgcc
     8941 cgatgcctcg attacttgag gggcaaatct catataaaaa tcatgcatgg cttggtcagc
     9001 cactgctaat tcattttctt tcaagccttt ttgtggtccg aaaacaaaag aagcgccttc
     9061 ttttccgcat aaaagattag taacatcagt aatgacagtg attttggctt taatcagctc
     9121 aataggcacg ttcgttttat caactgtagc aattttacct agattttctc ccagtgcttc
     9181 tactttttga ttatcttgat cgtaaaaagc ataacctaga ccttctgcca tgccgatgcc
     9241 gccatcgttg gtactacttc cgccaacgcc gatgatgatg tgtcgaacac cttgttgcaa
     9301 caattgtcgt atcagttctc ctactcctcg cgtggataac ctaagtggct gttgttggtt
     9361 tagtgggaca tctttcaatc cgacgatttc tgccatttca aaaatcgcag tttgaccgtc
     9421 tgaagcgtac tggatttctt tgtttttgcc aaatgcatta gtaacgactg ctgtttgccg
     9481 cttcaacctt aaactgtctg ctaatgatga aaccgttcct tctcctccat cgcctatagg
     9541 tagcagatca tattcagcct gaacaaaaac ttcttgaaaa cctgattgaa tggcttgtgc
     9601 tgcctctaag gctgtcagac tttctttgaa agaatctggc gcaatcagaa tacgcattat
     9661 tttcccctcc gttccaagat cagttctttt tccagctggt attggtctcc attgacaaaa
     9721 ataggtaatg tttctttcgt ctcttttttt attactattt gtttttttgt caaaacaaat
     9781 agcaacctgt gatttttcca aataaaagga aaagaaattt tttcccatgc tgaaggaagt
     9841 cttggagaaa tagaaagagt tccattcgtt actgtaatac ctgaaaaacc aaatataact
     9901 gctagccata gtgctcctaa tgaagcagca tgaattccgt catctgatga atgcggatct
     9961 ggtcctaaat caatcaggca cgcttcttgg aataaatgat acgcccattc catttcttgg
    10021 atacggcaag cctctatggc gtgaatcgct ttactcaacg atgagtcgtg gattgttttg
    10081 ttttcatagt aatgcaaatt cttagaaacg attggttgag aaaaatactc aggtaataag
    10141 tataacagca tgacaagatc tgcttgcttg ctgacttgga gttcatttac ttcgctacgg
    10201 gcataatcaa gtaaaataga ctggctgcca ggacgttctt tgtaagtggt caaatcaata
    10261 actggcttac tgagatacgt gtcatcttgc ggaatcagct gttctttgtt aggcactggc
    10321 aaatataact tatctaaaaa attttgacat tctttcaaaa aaagcggatc gccagcttga
    10381 tatttaacag ctaaggccac attataatgc gccagatagt tcgtatatgc attattgtcg
    10441 atatattctg tgtattcatc tggaccaatg actctgtgta tctctaaatg atcatcgact
    10501 tttacagctc tgctcagcca aaaagctgct gtttcatgaa tgagcgtttt tccctttgtt
    10561 tctaaaaacg attgatcctc tgttgcttgg acatactctt gaacggcata agcaaggtct
    10621 gcactgatat gatgttctgc ggcagctgaa gcaacttctt gtcttgttcc ggttttgata
    10681 ttcaatgcag caaatttggg tgtctcttcc tgaccagtgt gtgcgctttc ccatgggaaa
    10741 agtgcacctt gatagtgata atctcttgct ttttgccgtg cgccttctaa tcgatcataa
    10801 cggtacagca gcaaattctt tgctacttca ggaaactgat ataaaaagaa aggcaggata
    10861 aaaatttccg tatcccagaa aacatgccct ttataacctt cgccagttag tcccttcgcc
    10921 cctacactaa cacttggatc attgcgggga gtcataatct gtaaatggta agcagcgaag
    10981 tccatcgcta actgatcgaa agaagaatcg ccttggatcg ttatccgatg ctgatgccaa
    11041 aaatctgtcc aataagacat cgactgctct aaaatttctg tataactaac gcttttctgg
    11101 gcttgggcaa tacttttctt aaacgggtct gtttgctgaa gagaggtaga gagcacgatg
    11161 atcttctcca gcgtaaagga cgtatttttt ctgatctttt ctgaaaagga agcggtgatc
    11221 cggcgatttt ttgcttggaa tacacctgct ttattgaatt tagctgcaat cccaatatca
    11281 attttacttt ctgttgtctg gtaggatgca ctgatcaaat cgtcaccaaa tactcgcagt
    11341 tctttttcaa ccaactgctg tgtaccaaaa ttggtttgct gtccatcgat acccgttttc
    11401 acatctactt ggcagtcttc ggtcaacggc ttgatgacca ctttcagggc tgcctgatgg
    11461 agattatttt gatcagcaac acgacaagta atgacttcca ctttttttcc ttgagcagtt
    11521 tcaatgagcg catagcgttt aagctctccc atttctaaat ctagtgcccg ctcgtatgca
    11581 agaactttca tcgtctcaag agaaaaatgt tcaccgtcga tttttatttc ccatctgatg
    11641 acatctggga gattcactaa ctcggctgat tctgctccta aaggtcggtt atatacacca
    11701 gctgcaaaca ttccccttac ttgattccga tagtcttcct cctggcttcc tctgatacca
    11761 aaaaatccat ttccaatggt catctgactc gcatacttat tgatatactc taaatcaaac
    11821 tgtttttctt ctactttcat aaggtaataa cttctcctgt ttctgccgaa cgatataaat
    11881 tttccaccag attctggatg acatatcctt gccagccatc tgcaatcatc tgagtctggt
    11941 caccgagcgc ccgattcaca aaatgagcca tacttagttc atgtgggtca ttaactggcg
    12001 tggtcttttg taaggaaacc agctttccat ctacatcttc gaatagttcg cctggataca
    12061 gattagcacc tgcctgatcg ccacaaaaag ccacattcag tttgtcttct ctgatattca
    12121 aagcaaacga agtattcagt tggatgatct tgccgttttc taattccagt gtgccaaaaa
    12181 ttgcatcttc tactgtgaat ttgcttggat cccatttccc gaatttccct gaattttttt
    12241 tcgtaccaat tttttggaag ctgtacgccc gaacgctttt tactttagga aatccagtaa
    12301 tatacatcgc tgtatcaagc atgtgaatgc cataatcgat caaaggcccg cctccttgaa
    12361 gttctttgtc gataaacgtt ccccagcctg gaacaccaga ccgacgaagc gcattcgctt
    12421 ctacgtaata aatatccccg agaacatcaa gatgattctt gatcattgtt gcttcgtcag
    12481 aaaaacgatg ctggaatcca taagccaaaa tctgcccgtt tttatcggct gtctctgcca
    12541 tctttcttgc ttgatcagct gtcatagctg gtggtttctc gcacatcact gaagcattag
    12601 cttcgagggc agccaataca tgttcgaagt gaaagcgatt gggtgtacag acactcacga
    12661 tatcgggaga aatctcattc agcatttgtg taactgatgt atacgcatga gaaaatcctg
    12721 tacgtcggca aaagtcttct gctcgctggc tgactggatc aaccacagcg gccaattcta
    12781 agacatctga tcttttttga taataagcag catgtacttt ttctgctact tgtccagcgc
    12841 ctatgattgc tactttcttc atttatttct ctctcctttg cttatttact tattgttatt
    12901 ctttctctat tctttctcta aatcatttaa atgacgcgct gtttccagac agtttttcaa
    12961 gtattgcaat gattcctgat aaattttcac ttcattttct cctcgcaaac gacattcata
    13021 taccatataa ccttcatact cattttgaag tagcgtttga aaatggcgag taaaatcaat
    13081 tgcgccagat cctggttggt agcgatgatt atctgctaaa tggatatgac ccacatactc
    13141 tttttgcgta gtcaatgctt ttgaaatatc atcttcttca atattcatat gataaaaatc
    13201 agcgatgatc tgcacatgtt tcaattgatt agagtcaatg taatgacgcg catccgctaa
    13261 caaattgatc atatggtctt ggtatcggtt caaaggctcc aaataaataa ccgtacctgt
    13321 ttcttcggct actttatcta aataacgaag tgattcgctg actgctttat agtctccttc
    13381 tttgcttcgg ggtgaaacca tcggcggtaa tcgataagtg aacattcccc aagctgctgg
    13441 gataacgatt cctttgcctc caacctcgct tagtgcacgc aaaatctcag caatttgacg
    13501 tacaccatta agacgccgtt cttcgataaa gtcaccgatc cacccatcgt agccgttaca
    13561 agctgtcact actgggatcc ctgtttcttt gattgcctgt ttgactgtgt cgatattttc
    13621 tattaacaac ttaccgtcga tttcgtattc ttcaaatcca cagtctttga taaatctaaa
    13681 tttttctaaa atgtctcttg ggaaaaatgc ttgatcttgc gtaccgaatt tcattgttct
    13741 cctcctcgga cttgaacgcc cattttgata ctttcttctg ggtgctggtc tacataacgc
    13801 atgtaagctt ctgcgctttc tgcaaatggc acaactggat caatgatttc ttcacaatcc
    13861 aagtaaccgt tcatcaataa ttcccaacat gtgtcctcga ttcgtttacg gttccatctt
    13921 gggtaatctg ggtttggttc actagatgct cgagcaaaga cgatttttgc attattgaaa
    13981 tgtgcttcgc gaccaagatt aaacccagct ggaaaaggtt tagcaaaggc aacataagaa
    14041 atgatcccgc catagccgat ccctattaaa gaagcttgta atgcggcagc actgccactc
    14101 gtttcaataa ctgaatctac tccacgttta tctgtcaatt cctttagacg aaaaccgaca
    14161 tctgctttca taggatctag cgtgtaatcg gcaccgtgtt tttctgctaa ttctctacgc
    14221 tgcgctatgg gatcgatccc aacgatggta gtagcacctg cttttttagc taattgaatc
    14281 gcaatttgcc cgatagcgcc taaaccgaag attgctacag catcacctgc tcgaacatgt
    14341 gcttcccgga taccacctaa agcgaattga gcaggatcgt aacatactgc atttttccat
    14401 tgatccaacc gatcaagttt acgtagttta taattatcaa caccgtttgt aatcagcgtt
    14461 tcacgaatgg gtccataagt gcagactaac tctccttttt gatactctgt caccaaactg
    14521 cccgtttctt cgatcgtacc gacaatcata tttcctaatt gaaattcgcc aaataccaca
    14581 ccgcgttggc ttccttcttc tcttggcaag aataggcgcc attcttctga aaattcttca
    14641 tcgataaatg gacttaatcc tctaaagtcg acaatttctg ttcctggttt tggagtagcg
    14701 aaaattactc ggatacgtac ttcgttcttt tcgatctcac ggtcttcgta atctttgaat
    14761 ccggctattc ttggttctaa tgcaactaat tttttcatct tttctttcct ctttcttttc
    14821 tttttatcct tttacgccgc cttcgactaa atcaccttta ataaatcgtt cagataacgc
    14881 atacataatg atgactggta aagcggtcac caatgaagcg gccatcattc gtccccaaat
    14941 gtagtctggt gtactgaata atgtgttcaa accgattggt aaagtaaatt tttcggaggt
    15001 cgacaggaaa atcgaagcaa ataaataatc attccacgaa accataaagc aataaacgaa
    15061 gacagaaatg attccagaaa tagccaatgg tataatgatc cgaataatga tctggatccg
    15121 gctaagacca tcgatcattg ctgcttcttc aatatcagtc ggaatcgtgt caaaataact
    15181 ttttagcatg aatatcgcag taggaagtgt ttgaacgacc atcgtgataa tcagtgctgt
    15241 attggtatca tacaaaccta gaccggaaat gattttaaat aatggaacaa cgatcaaaat
    15301 accggaaaac atataaacgg tgtaaaagct agcattgatt ttcgagcgag ctttgaagcg
    15361 tagttttgac aaagcgtatg ccccgctgat cccaatcact actgcagtta cggaggctat
    15421 caaggagatc actaaactat ttttgaaata catgatgaat ggaaaaatct cagcattgaa
    15481 aatatcctga taatgttcaa aagtccattc tttcggaaaa atagtttgtg tcgttgcaat
    15541 tgcttcttgt gaatccttga atgatgtcat cagcatgatc aaaaatggaa acaaattgac
    15601 agctaatagc aacaaaatcg tcgcgtaaaa taagattttc ctgctctttt tatttttcat
    15661 ttcaagtcca ccttctttcg tgagagcaag acgaccgctg caataatgat gaacaataga
    15721 atcgatatag ctgctgcttt tccaagatca ttgaacgcaa aggctgtctg atacaaataa
    15781 acgcctaaga tattgacttt attcgtcagt agatagacat ctgtaaacat atagaacatc
    15841 cagatagatc gtaaaacaat cacagttgct agtactggtt tgattgccgg cagtgtcacg
    15901 atcttgaact tctgccaagc atttgcaccg tccatttcag ctgcttcata aagcgttttg
    15961 tcgactgttt gcaaaatagc caaaaatgaa ataaacgcat acggatagta tctccagata
    16021 gcaaacaaaa cgaccataaa gaagctagca actggctggt cgaaccacag cggtgcttcg
    16081 ttgaaaagct gcaacctatc tactaacaga tcattgacga ttccataact attattgaac
    16141 atatatttcc aagcaaaaat caaagaaata gatggcgtaa cataacttaa taaaatcagt
    16201 gaacgcaccg ttttcctaaa tctgaacttg cggttaagca aaatagctac ggctaatcct
    16261 aaagctgtac tgcctgctac aactaagaat gtgtaagcaa ctgttaaacc taaagaacga
    16321 taaaattcac tatcacttaa aatttcaaga taattgctta atccgataaa aacggcgtct
    16381 gagttaggat tcagcggcat atcgaaaaaa ctgatctgaa tattggaaag cattggaatc
    16441 agcactaaaa cagctaataa aagcatgctt ggtgctaaaa gtcccatggc taattttaag
    16501 tcagaatgtt tccttggacg attcattgaa acctccccct gataatctgc caatagctat
    16561 ctactttttt cgaacaccat tggcagaact tcttgttatt tcgtattttt ttgtgcgtca
    16621 gctaaagctt gatcgacatc ttcgtttccg acagttactt ggttcactgc ttttccgatc
    16681 acacctgagc ttgtgatatc tcccattttc gtgaaattct tttccccgac tagtccaaag
    16741 acttgtactt catcaaaagc actagcaatc tcagatggca gctcgccaaa agacttgatg
    16801 acttcatttt ctttgtaacc atccagttcg actacttgtt tattcactgg ttgagcacca
    16861 ccaggagaca tcaagaccca cttttccatg tttttcggtt gagataagaa agctgtgaat
    16921 gcttcagcag cctctttttg tttctcgtct agaccagaag aaatcgtcaa tccagatact
    16981 gtgccataga cagcttgatc tttttgtgtt gggatagcaa aaccgatatt ttctgagttc
    17041 cccgcttcat aaacagaagg caaaatgtaa gttgaataga ttgccattgg tgctgttcca
    17101 ttcatgaaag catctttgat ttctgtcaca tcattagagc ctggcatcgt gtattgggat
    17161 aagttttgat aaaacgacaa tgcttctctc atcttttccg tatcgatcgt tacctcgcct
    17221 tcatcatcca aaacatttgc tccatttgac aaagcaaatt gagagaacgc ttgctcactc
    17281 attgttcctt cagcagtagg catagcaatt ccatattgtt tattctcttt atctgtgaaa
    17341 tgttgagcaa tttttaaaat atcatcccaa ttttctggtt ctgaaaaacc agcttctgat
    17401 aatttttctt tgtcatacca aataccttga acccagccgc taattggtgc agcaatatag
    17461 ctttttccat cttctgaacg aacaaggttt ttggctccgt cataataatt atcttctcca
    17521 gcttcctcga taacgttttg gactgccgtc tgatcaatca gttcatcttt atccatcact
    17581 ttagcaaaat cctgactgac ttccattact tctggtaatt taccggaacg agctaacgta
    17641 acgatttttg tattgaacgc tgattcttct accggcactt gcttgatttt gatgtctgga
    17701 ttttcttttt caaaatcagc aaccagttca ttgatcacac ttaatcgttc ctgttcgaca
    17761 gaagagtgca tgaattgaat cgttgtctgt tgtgattcgt tagagctttg actttcacca
    17821 caagcagtaa gaagaaatcc agtagctagc agacaggtag taacaataat ttttttcatt
    17881 tttttccacc tcttatttta ttattttcag ccacatatat tcatacggct ggaatatcaa
    17941 ctttcgttct tgactgcaat ggataatttg tccagaaaac aggtctaggt aatctccttt
    18001 tggtaaaaga taacctgctg attgattgga aaaattatgg ataactaaga ctttctcttc
    18061 cttcgcttcg cgaataaaac tgataaaaga atctgctgag tccagtaagt aaatcaaact
    18121 attcggatgg aaggcttttt ctttttttcg aatagatagc atctgggtca attcaccata
    18181 tactgctgtt cttaatggat ctgtctgcaa ctctttctca atatctgcta acaaaaattt
    18241 ttgccgattg atttttcgat tctctcctgt tttttctacg cctttgtaat catttcgact
    18301 tcccagcaca ctttgaatat aaattgccgg cacgcctggt aacgttacca aaaatgaatg
    18361 agcagctaaa aagcgtttgc ttctccattc cgtcgtatct gttggagaac ttaacgcatc
    18421 tagatacgta gcgtttatct catatggact ggtcgttcca tctgggtttt tcttgtagtt
    18481 tactcgggca ccctctttct tcagctgttc cactaattca aggatcgttt tttccggcag
    18541 aatacctcgt accggattca atccgatacc gtcatgggag gctaaaaaat taaaaaaagt
    18601 tgtttttttc gatgcgaaat cgatggtttt taaccattct ttcaacttac ttgtattacc
    18661 cgaacgaatc gcttgaaggg tcaaaggtgg tagcgaaaac tgatagacca tctgtgcttc
    18721 attctcgcca ttcccaaaat aactgatatt atctttgtgt ggcacatttg tctcagtgat
    18781 caataccgtt cctggagctg cttcatcagc gattgcacgg aataattgga tgattttatg
    18841 cgtttctggc aggtgtatac aagaagttcc tagttctttc cataaaaatc ccacagcgtc
    18901 caagcgaata aatgaagcgc ctttttctaa gtaaaacaaa agaatatcga tcattttcaa
    18961 taaaacttct ggatttttaa aattcaagtc tacttgatct tcgctaaaag tcgtccatac
    19021 gtgtttttct ttcccgtttt tgtcaatgaa cgttgtaagt aacgggctgg tccttggtct
    19081 agtcacgctg cttgtatctg tatccagtgg caaatcaata aaaaaatctt tgtacgtatc
    19141 attcaagttt aaatattcct gaaaccaagt actttcggca gacatatgat tgcagacaaa
    19201 gtcgaacatt aaccgtgttt cgcttctcat ttcttctata tcttcccatg tgcccaaacg
    19261 gtcgttgatt gcctgataat ttacaacgga gaaaccatca tcagatgtat atgggaaaaa
    19321 tggcaagaaa tgtacaactc taaatgtatc tgccaaatac ttttgataaa atttcttaaa
    19381 tgtacgcaaa gttggttgct gctcttcaaa aaattgatcc ccataagtaa tcaagaaaat
    19441 atcttcttcg tcccaatgca gtttgcctgc ctggcctttc ttctgctgat aaacactgac
    19501 tttttgttca atcatagagg cggttttctc agacaaatct gagtcgcgat aaatcgcttg
    19561 taaaagtttt gtatacatcg gattcccctt tcttttttgg gagcgttccc aacgatttga
    19621 tcatattatg gaaaacgctt actgtcaata gcattttttt aaataatttt gatatacaca
    19681 aaaatacaaa taagaaagct tatgtatcaa taattaatag cttcttaaaa aaatatggtc
    19741 tctcatgatc atgcaagcta ttcctaaatt ttcaaaatca tagacaaaaa agaatgtcta
    19801 ttgtactatg gaattattct aaaacgctcc caaaaaggag gtgcatataa aattgaaacc
    19861 tactatttat gacattgcga aaaaagctaa tgtctcaaaa tcaactgtct ccagagtatt
    19921 aaataaccag ccaaatatct ctgataaagc aaaagcaagt gtacttcaag caatcaaaga
    19981 attgaattat gaaccgagca aaattgcaag aggactgtca ggaaatggtt ttgacgcgat
    20041 tctagcatta tctcatcgta cttctcatac gacctatggc aatccttttt tttcagatgt
    20101 tattcaatcc atttctgccg tttctgaaca aaataacttc gacttgattc tacaaacggc
    20161 aaaaacacca gaagaagagc tagaaaaatg cgcgaaaaag atccaagaaa aaatgatccg
    20221 aggaattatt attttaagct ctacgatgga tgaaacactt ctttttgaat tggatcaata
    20281 caatattcct attgtggtaa tcggaaaagt cggaaacgac tacacgaaca tctactcagt
    20341 ggacacggat aatttccagg atagttacga tttggtggaa acactgatca agaatggaca
    20401 ccgtcatatc ggctgtctcc atgctccgac agaagtttat gtatccaatg atcgtctcaa
    20461 tggttaccgt caatgcttat ttgatcacca cctagatatc cgtaatgaat ggattataga
    20521 cggcggcttt cgcttagaag atagcatggc agcaatgaat gatttaatga agctacgaga
    20581 aagaccaact gcgatttttt ctaccgatgc attaaaatca ttgagtattt acaaagtggc
    20641 agaacaatct ggaaagcaga tacctgatga tctttcgatc gcaggattca gtgatccctt
    20701 attgtcctcc ctattttcgc cacagttgac tcggatagac attccgacaa atcagttagg
    20761 aatcaaagcc acagaacttt tattccaact aatccaccac caataccctg aagaaaaaag
    20821 aatcattctt tcaaccgaga ttactctcac agattcgatc caacaacaag aaatgtagaa
    20881 cagaattata aaagaacaga aacactgagg ataagtcagt tactctgttc ttttttcaat
    20941 cactcatccc tcgttacccc ctcccggtat tttagcgaat ttttcttatt ttccatctgt
    21001 tttagatagc tgtggataac tataatgttt gtgttaaact attttttcca cagggaagaa
    21061 aaaactattt ttaaccacgt tgtgtataag tttttatagt tcgttttttt aaacacattt
    21121 acccttattt caatccatgt tatttgggga tacttatctc taaaacataa atagaataac
    21181 agacatgtct actggttttg ttttattcga ggaattattc aataatagct agcaaaatat
    21241 taaaaataac cgctctatct tcccatcaat ttattttgca ttcaaaaaat gatctatctt
    21301 tacttttgtt gattgacttc tcttggaaga tgatcgttcc gataccttaa ttggatacga
    21361 accgatgttg ttaagatcct ttcccacttt tcttctagtt agacaaatta ttttagacaa
    21421 accaaaaaag aggttgtgac aataatttgt cacaacctct tttttaaaat atctattttg
    21481 tttgattgtt cattgcgtct tctgcagcca tttgcgcttc aatatctgaa atactgtaga
    21541 cattttcgct tgctacaccg acttcttttg gttccatgtc acgataacgt tgcataccag
    21601 taccagctgg gatgatctta cctataataa cattttcttt caaaccaaga agaggatctt
    21661 tcttaccgcg gatcgcagca tccgtcaaga cacgagttgt ttcttggaat gatgcagcag
    21721 ataagaaact atttgtttct aatgatgctt ttgtgatacc tagtaagaca ggtcgagaag
    21781 ttgcaggaac gcctcctgag actagcgtat tgtagttttg atctttaaag tctgcgatgt
    21841 ctaataatgt acctggtaag atatctgtat cacctggatc cattacacga actttacgca
    21901 gcatttgacg aaccattact tcgatatgct tgtcgccgat ttctacccct tgcatacggt
    21961 aaacacgttg tacttcacga agcaagtagt tttcaacaga taatacatcg cgaacttgca
    22021 acaattgttt tggatcgatt gatccttctg tcaatggtgc accacggtgg atcatatcgc
    22081 cttctgctac tttcatacgt gctgtataag gaacagtata cgtacgtgta tctgtttttc
    22141 ctttgattgt tacttctttg gtacgtgttg caggatcttc tgagatatcg atgacatccc
    22201 ctgttacttc tgtgatcact gcttgccctt tcggattacg tgcttcaaag atttcttgga
    22261 cacgaggaag accttgagtg atatcgtctc cggcaacccc acctgtatgg aatgtacgca
    22321 tcgtcaactg agtaccaggt tcaccgattg attgagcggc gatcgtacca actgcttctc
    22381 cgacttcaac gtcagagcca gttgcaaggt tgcgtccgta acaatgtttg cagacaccat
    22441 gttttgtatt acatgtgaag acagaacgga ttgtcactgt ttcgacacct gcatcaacga
    22501 tttgttttgc gatgtcttcg gaaatcaatt ggtttcctgc aatgatcaat tcatttgttt
    22561 caggatgacg tacttcttta cgggtgtaac gtcccactaa acgatcttct aatgattcga
    22621 tgatttcatt tccctcgcgg atggcttgga tttcaagacc tcggtcagta ccgcaatctt
    22681 cttcacggat gattacatct tgggccacgt caaccagacg acgagtcaag taacctgaat
    22741 cggcagtctt caaggctgta tcggtcatcc ctttacgagc cccgtgagtt gagataaaca
    22801 tttccaagac ggatagtcct tcacggaagt tcgaaatgat tggcaattcc atgattcgac
    22861 cattaggagc ggccatcaaa ccacgcattc cggcaagctg tgtaaagttg gaaatgttac
    22921 cacgagctcc agaatcagac atcatgaaga ttggattttt cgcttccaaa ccttccatca
    22981 gtttttgttg gatgctgtct tttgcagcat tccaaacagc aatgacacgt tcataacgtt
    23041 cgtcatctgt gatcagacca cgacggaatt gttttgtaat agtttcaact tgtttatggg
    23101 cattttcaat gatttcttgt ttttcattca gtacactgat gtcagcgatc cctactgtga
    23161 taccggcatg tgtcgaatgt ttgtacccta agtctttcat acggtcaagc atcttagaag
    23221 tttctgttac tttgaatcgt ttgaagactt ctgcgatgat gtttcctaag tttttcttct
    23281 tgaacggtaa tactaattct tgctctttga tatgtgcagg aatatcagta ccagcttcaa
    23341 caaagtattt gtccggtgtt tggacggtca agttgaagtc tgttggttca ttcagataag
    23401 ggaattcagg aggcatgatc tcgttgaaga tgatcttacc aactgttgtg atcatcgtac
    23461 gttctttttg ccattcagta aatggttttt cacctaatga gttagggttc acaccgatac
    23521 gtgaatgtaa atgaacatat ccgttgcgcc atgcgataac ggcttcgttt tgatctctga
    23581 agaccattcc ttcgccttcg cgtccttctt cttccattgt caagtagtag ttacctagaa
    23641 ccatatcttg agaaggagta acaactggtt taccatcttt agggttcaag atgttttgag
    23701 cagctaacat caacatacgt gcttctgctt gtgcttcttc attcaatgga acgtggacag
    23761 ccatttggtc tccatcgaaa tcggcattat aagcttcaca tacaagtgga tgcaaacgga
    23821 ttgcgcgtcc ttgtactaaa actggttcga atgcttggat accaagtcgg tgaagcgtag
    23881 gtgcccggtt cagcaatact gggtgttcct tgattacgtc ttctaaaaca tcccatactt
    23941 cgtcttcttg acgttcgatt ttacgtttcg catttttgat gtttgaagct agttcgcgtt
    24001 gaacaagctc acgcatcacg aatggtttga ataattcaat ggccatttct tttggaagtc
    24061 cacattggta catcttcaag aaaggtccaa caacgataac ggaacgacct gagtagtcga
    24121 cacgtttacc tagcaagttt tgacggaaac gaccttgttt ccctttcaac atatgagaaa
    24181 gagatttcaa tgggcggtta cctggtccag taactggacg tccgcgacga ccgttatcga
    24241 tcaatgcatc tactgcttct tgcagcatac gtttttcatt ttgaacgatg attccaggtg
    24301 cattcaagtc taacaaacgt ttcaaacggt tattacggtt gattacacga cggtaaagat
    24361 cattcaagtc acttgttgca aaacgtccac cttctaattg aaccattggg cgtaaatctg
    24421 gtggaataac agggataacg tccataacca tccagcttgg ttgatttcct gaagcgcgga
    24481 atgcttctaa aatatccaaa cggcggattg cacgtgtacg tttttgtcct tgtgcagatt
    24541 tcaattcttc tttcaattcg gctgcttctg catctagatc aacattatca agtaattgtt
    24601 taatcgcttc tgcacccata gcagcttgga attcttgtcc atattgttca cgtttttcgc
    24661 gatattcgcg ttctgtcaac aattgttttt tctctaaatt cgtatttcct ggttcgatga
    24721 ctacatatga agcaaaataa atgatttctt ccaaagcgcg tgggctcatg tctaatacaa
    24781 gacccatacg tgaaggaatc cctttaaagt accagatatg tgaaacagga gctgctaatt
    24841 caatatgtcc catgcgttca cggcgaactt tcgaacgagt aacttccact ccgcatcgat
    24901 cacaaacgat tcctttataa cggatacgtt tgtattttcc gcaggcacat tcccagtctt
    24961 ttgtaggacc aaaaatccgc tcacagaaca acccttcgcg ttcaggtttt aaggtacgat
    25021 agttaatcgt ctcaggtttt tttacttcac cgtaagacca gcttctgatt ttttcgggag
    25081 aagccaaacc tatttgcata ctttcgaatt tatttacatc gatcaaaagg ggttccctcc
    25141 atttcattta atggcaaacc gcaagacggc tgatcttcga ccgccttgca gctcatagac
    25201 cggattagtc taaattatct tctgcggttt caaagttttg gatgacatcg tctttttcat
    25261 cttccacttg ttcagcagct ttcttttcta attctttggc agtttgctgt tcggcaaatt
    25321 ttgtcaaagc atcaacagtg atcaaatcgt cgtcttcatc gtccatgtcg cgaagttcga
    25381 tctctgtttc ttcgatatcc aagacacgca tgtccagacc tagtgattgc aattctttca
    25441 ccaatacgcg gaatgattcc ggtacacctg gttttggaat tggttcaccc ttgacgattg
    25501 cttcgtatgt tttcacacgt ccgactacgt catctgattt gtatgtcaag atttcttgta
    25561 aagtataagc cgcaccgtaa gcttccagtg cccatacttc catttcacca aaacgttgtc
    25621 caccaaattg agctttacct cccaatggtt gttgtgtaac caatgagtaa ggtccgattg
    25681 aacgtgcatg caatttgtca tcgaccatgt gagcaagttt gatcatgtac atcacaccga
    25741 cagacacacg tccatcaaat ggttcacctg tacgtccgtc gtacagaaca gttttcgcat
    25801 cgctggccat accggcttca cgaactgttt cccatacgtc ttcatcactt gcaccatcaa
    25861 atactggcgt tgctacgtgg atacctaact gacgtgcagc catacccaag tgtaattcca
    25921 atacttgccc gatgttcata cgtgaaggca cccctaaagg attcagcatg atatcgattg
    25981 gtgttccatc aggtaggaat ggcatatctt cttccggcat aatacgggat acaacccctt
    26041 tatttccgtg acgaccggcc attttatccc cttcatggat tttacgtttt tgaacgatat
    26101 aaacacgaac taacatgttt acgcctggtg acaattcatc tccagcttca cgagtaaaga
    26161 ttttcacatc gtgaacgata ccgccgccgc catgtggtac acgtaatgat gtatcgcgga
    26221 cttcacgagc tttttcgcca aagatcgcat gcaatagacg ttcttctgca gataattctg
    26281 ttaccccttt tggcgtgact ttccctacaa gcaaatcgcc atcttggact tctgcaccaa
    26341 tacggatgat acccatttcg tctaaatctt ttaatgcgtc ttccccgaca ttagggattt
    26401 cgcgtgtgat ttcttcaggt cctaattttg tatcgcgggc ttctgattca tattcttcaa
    26461 tatggataga agtgtatacg tcatctttta ccaaacgacg gctcatgatg atcgcatctt
    26521 catagttgta cccttcccaa gtcatgaaac caactaaaac gttttggcct agagccattt
    26581 cgccttgttc catagatggt ccgtctgcca gagtatcccc tttttcgact ttctcaccca
    26641 aatgaacgat tggacgttgg ttgtagcttg ttccggagtt tgaacgacgg aattttgtta
    26701 catcgtattt atctaatgca ccgttgtcgc ggcgtacgcg gatttcagaa gcatcaacgt
    26761 attcaacgac accgtcatgt ttacataaca aagcagctcc tgagtcatgc gctgatttgt
    26821 attccatacc agtaccaacc catggtgctt gaggattgat caacggcact gcttgacgct
    26881 gcatgttggc acccatcaag gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg
    26941 ctgtcgcaac ggcaactact tgtttaggtg atacgtccat gtagtctact ttatcaatag
    27001 atacttctaa gttttcactt tgagcacgag ccataacaac gtcttgagca aatgttccat
    27061 cttcattcaa tgggctgttt gcttgggcta cgatgtaatg gtcttcgata tcagctgtca
    27121 agtagtcgat ttggtcagta acacgacctg tttcacggtc tacacgacga taaggtgttt
    27181 cgataaatcc gaatttattt actttcgcat aactggacaa actattgatc aacccgatgt
    27241 ttgggccttc aggagtttcg atcggacaca tacggccata gtgggagtag tgaacgtcac
    27301 gaacttcata accggcgcgg tcacgagtca atccgccagg tcctaaggct gaaagacgac
    27361 gtttgtgtgt caattcgcca agagggtttg tttgatccat gaactgtgac aactgagaag
    27421 aaccaaagaa ttctttgata cttgccacaa ctggacggat attgatcaat tgttgaggtg
    27481 tcaatgtttc agtatcttgg atcgacattc tttcacgaac cacacgttcc atacgtgcta
    27541 gcccgatacg gaattggttt tgtaaaagtt caccgactga acggatacga cgatttccta
    27601 agtggtcgat atcatcgaca ttgccgatac cttccatcaa gttcaagaaa tagctcattg
    27661 aagcaatgat gtcggctgga cgaaccgttt ttacagctgc ttctggatag ccgtttccga
    27721 tcacgtttac ttcacgttca gggtctcttg gtgaaaatac tttgatgact tggacagtca
    27781 ttggatctgt gaccacaccg tcttctgatg gatagtaagt cactgaattt aggccattat
    27841 caatgaaagg agctagtgtt tccatgactt ggtgagtcag aactgttcct ttttcaacga
    27901 tgatctcgcc tgtttctgga tcaactaatg tttcagctaa tgtcagattc aatagacgtg
    27961 ttttcaaatc taattttttg tttactttgt aacgaccaac gtttgctaag tcgtaacgtt
    28021 ttggatcgaa gaaacgagca ttcagcaagt tacgtgagct gtctgctgtt tttggttcgc
    28081 ctggacgcaa acgttcgtaa acgtctttca aaccttcttc tgttcttgag tcgcttgcgt
    28141 ttttatgcaa gtctttttcg actgtattgc gtaatgtttc gctgtcgccg aagatttcaa
    28201 agatcgtatc atcggaacca aatcctaacg cacgaactaa tacggttaaa ggaatcttac
    28261 gtgtacggtc gatacgtaca taagagatgt cttttgcatc agtttccatt tctagccaag
    28321 ctccacggtt agggataaca gtagaaccga atccttcttt tccgtttttg tcgacttttc
    28381 catggaaata gacacctggt gaacggacta actgagaaac gattactcgc tctgccccgt
    28441 tgatgatgaa tgttccttgt tctgtcatta atgggaaatc accgaagaat acttcttgtg
    28501 ctttgatttc gccagtctcg cggtttgtca aacgcaatgt tacatgtaat ggtgcagagt
    28561 agtttgcatc atgcgcacga gcttctgcta ctgtatactt tggttctttc aattcataat
    28621 ctacaaattc caaagataga ttgccattga agtcatcgat tggcaaaatg tcttcaaaca
    28681 tttctctaag tccctcatct aagaaccatt ggtaagagtc tgtttgaatt tcgatcaaat
    28741 ttggtaattc caacacttca ctgattcttg cgaaacttct acgttcgcga tgttttccgt
    28801 attttactac gtgtccagcc aagctcttca cccctctaaa atgtcattca aaaagactac
    28861 ccgatcaatt gttacaataa tatttcaaat attattttta ggtaacaaca gacatttttt
    28921 gcggctttta ggcaaaaaaa aaccaaaaat agaacattct cttgaaacct tacttcgtga
    28981 gcttctactt ttgtttcctt atattaaagc cgaaacggac aatatttcgt ttctgccttt
    29041 tcttcatcag atagtttttg caacttcata tactacactt attttctata aaagtcaaca
    29101 cttcctggcg tttcttctgc tttttcgcaa cattttctta atgttttcaa ttttatgttt
    29161 tcaattatct gacaattctt aattgacaga cctttttatc tcggttatac tttagaaaat
    29221 tcattttgtg gaggaacttc tatgcgaacc aacatcaacc aaatcgcacc aacacaactg
    29281 aataactttt actttagcta ctttagatag gtttgtattc atcatgatgg atacaaacgt
    29341 taaacgacag tttgtatcta tgatggctaa agaactttgt attgcagcaa aaagccacgt
    29401 agacaaaatg ctaagtggct gagcagtgaa aatacaaagt aaaagtatca agcattctac
    29461 tttttcaatt tattttccga cttttcaatc catttggaca ttttgtctga atggattttt
    29521 tgtttcccaa aattaaggag gaaaaagaaa tgaaaaaaaa tataatcatc ggattaggcg
    29581 ctgccctgct actagcttta ggaggttgcg cacaaaaaga caattccgca gaggcatcag
    29641 atggacaaaa aacaattggc gtattgcaac ttgtacaaca taactcgtta gatgctgcct
    29701 ataaaggatt caaagaagga ttagcagaag aagggtataa agaaggcgat aatctaacaa
    29761 tcgattatca aaatgcccaa aacaatcaag acaacctgaa aagtatgagt gagaaactcg
    29821 tcaaagaaga tcctgatttg ctacttggaa tcgccactcc agcagcacaa tctttattaa
    29881 atgaaacaac cactatcccg atgactgtca cagcggtcac agacttggaa caagcccatt
    29941 tagtcacatc caatgaaaaa cctggcggca atgtgactgg cacgacagat atggtgccaa
    30001 tcgaaaaaca aatcggctta ctgttaaata tcgtgccaga cgccaaaaca atcggcatca
    30061 tgtataacaa cggtgaagca aactctaaga tccaagctga tttggcagaa aaagaattga
    30121 aaaaagcggg cgtcaaagtc aaagtactga cagcaaattc cacaaatgat gtccaacaag
    30181 tgacgaaaag tctagctaaa gatgtagatg gaatctatat tcctactgat aacacgttcg
    30241 caagtgcggc aagtgttgtc ggtgaagtcg caaaagaaac aaaaacacct gtcgttgctg
    30301 gcgcagttga tcaagtcaaa gacggcggtc tagcaacagt aggaatcgac tacgaaagct
    30361 tgggcaaaca aacagggatc atggcagcaa aaatcttaga cggcaagaat ccaagtgaac
    30421 ttcctgtcga aacagcgaat gatttggaac tgtatgtcaa taaagacatg gccaaagcat
    30481 taggcatcaa tccagacagc atcaaagaag ctgaataaaa cacaggagga ttctcatgac
    30541 aaatttgctc tctcttttct tatcatctgc atcgcaaggg gtcttatggg ccctacttgc
    30601 gattggtgta tacgtcactt tccgtatctt ggacattgcc gacttgactg ctgaaggaag
    30661 ctttccttta ggtgcaggga tcgcggcagt aagtattaca aatggctatc atccattgac
    30721 agcttgtctt ctaggctttt tagggggtgc ggcagcaggg cttgtttccg gactgcttca
    30781 cacaaaattg aaaattcccg cccttcttgc agggatcatc accatgactg gtttatattc
    30841 tgtcacatcc cgtgtgatgg gtgcgccgaa catctcttta ttgggtcagc caacgatctt
    30901 tcattgggtc gagtcattcg gcataacaaa agacaacgct gtactgatcg tcggtctgct
    30961 ttttgctttg atcgttgtat gtttacttgt tttgttctta cgtacagaaa caggacttgc
    31021 tattcgtgca actggcgata atctggcaat gagcgaagca aacgggatca acacagataa
    31081 tatgaagatc atcggttata tgatttctaa tggatgcatt gctctttctg gctccttact
    31141 tgcgcaaaac aacggctttg ctgatttaaa ttctggtatt ggaacgatcg taattggact
    31201 tgcttctatt atcatcgcag aagttttgtt acgtaaccag ccactatttc tccgtctctt
    31261 gactgtcgtt ttaggtgcgg tcatttatcg ttttatttta gcgttagtct tcgaactgaa
    31321 tgttgaacca agtgattcga aacttgcttc tgctttcgtg ctagtgatct gtctttcttt
    31381 cccgcagatc cagcaaaaat tccatttacc tcaaaaaaag aaaggcggta gtgtcaaatg
    31441 aatgacgtct taacattgga aaatctccat catacctttg aaaaaggaac gatcaacgaa
    31501 aatcatgtcc tgaaaggcat taatctttcc ttagatcccg gcgacttcgt gacgatcatc
    31561 ggtggaaatg gggctggtaa atctactttg ctgaacagca tcgccggtac attttctgtc
    31621 gatcagggtc aaattttatt gaatggaaaa aatatcacga aaaaatctgt tgtcgaacgt
    31681 tcgaaatcca tcagtcgcgt attccaagat cctaaactag ggacagccgt tcgtttaacg
    31741 gtagaagaaa atcttgctct tgctatgaaa cgaggaaaaa aacgcggctt cttccgtggt
    31801 gtcaaaccac aagaccgaac atttttcaaa gaccagctgg ttcggctaaa tttggggttg
    31861 gaaaatcgtt tatcctcaga aatcggtttg ctgtctggag gacaacgtca agctattacg
    31921 ttattgatgg ccacattaca gcgtccagaa ctgatcttat tagatgaaca cactgcagcg
    31981 cttgaccctg ctacttccaa aacggtgatg gaattgacag aaaagctgat cactgagcag
    32041 aatctcactg cttttatggt cactcatgac atggaagacg cgatccgcta tgggagtcga
    32101 ctgatcatgc tgcatcaagg aaggatcgtc gtagatatcc agcaagagga aaaacagcag
    32161 ctcacagtgc cccaattatt agcgttgttc aaacaaaaca gcggcacggc attaaaagac
    32221 gatcaagtac ttttatcttc ataatcagaa agaggatggg acaaaaaatg aacgcccacc
    32281 ctcttctttt ttctctttcc ttctttcgtt tttgctttat gtatcaaccg ttgtttcttc
    32341 gaaaactatt ttgttctctc cttcttcctg atagaatgga tgggaaagga gcttgatgcc
    32401 atgacaacga aagacaaggt acttcaatta ttgaaaaaga caccagattt tctttccgga
    32461 gaaaagttgg cacagcaact ggaagtttcc cggacaagta tttggaaagc catcaaagaa
    32521 ttggaaaagg caggctatcg ttttgagcac gggcctacag ggtatcgtta cctgccctct
    32581 gacgtactag atgcaaaaga aatccagcaa gagctacggt cgtttattcc tgatttgaaa
    32641 gtacaaacga tgttgacttc tgaatctacg atgaaagatg ccaaactggc agcagctcaa
    32701 ggagattctg tttcgaagtt atttgttgct gatacacaag aacaagctcg tggacgtttt
    32761 ggacggcctt ttttcgctga acctggacga ggaatctaca tgagtctctt gctccatcca
    32821 gacaaacggt ttgatgaatt gcctcaatat acgatcatcg cagccagtgc ctgtgtgaaa
    32881 gccattgaat ctgtaaccgg tcgttctgct tcgatcaaat gggtcaatga tatctacatg
    32941 gatggaaaaa aagtctgtgg gattttatct gaagcagtca gtgatatgga aagcggcagg
    33001 atcagtcata tcatcattgg gatcgggctt aatttttctg ttcctcaaaa tcattttcca
    33061 gctgatctcc aagaaaaagc catctctgtc tttcccgacg gacaagctag tgtcacaagg
    33121 aatcagctaa tcagcaaaat ctggcaagaa ttgttcactt atctttctca cccagaaaaa
    33181 gcgttggact tctataaaga aaaatcattc gtattgggaa aaaccgtgtc gttcaatcaa
    33241 aaaggaacga cttatgtcgg tcttgcaaaa gaaatcggtg atcatggcga actgatcgtc
    33301 caaacaaatg aacagaagat gagtttgtct tctggcgaaa tcagtctcac atctatccgt
    33361 acgcaataat tatcagattt cacaaaagga cacctgatct catacagtgt ccttttttct
    33421 gaattctctt caaaagactc tccttattct atcagctgac agtttctcta tcctctagaa
    33481 aaaggtattc taaaaatatc tataaagcat ggtacacttt ctttaaatga atcaatgcaa
    33541 aaaacaagag agtcatcagt ttacacgatc aaaagagata gcaggccgct tcctttttca
    33601 atcttataga tagaccatgg gtaacgataa aaacaaaaaa gaacttgcga cttattttgc
    33661 cgtaagttct tttttagcta gtgccttaat ctcttaatcc caacaaacgt ctcgcataga
    33721 tcacctgacc agagtgcata gcagcatcat caataataga aacgagacgc attcctcgtg
    33781 tgactggtgg tgtccagttc tcgtcaatta cttcatctag ctcggaaggt tccagccttc
    33841 ttaagtaaga aatagcaaaa tcagcagctc ctcgcaagta tcctagcaga tcttctattt
    33901 gattagcttg gatctgcttt gcttgttcca gactatgctt ccaatcttgt tcgttatctg
    33961 ctatcgtcat cggaaaccgg ctttcccagt cttgactatg ccaaattgga gaagttcctg
    34021 ctaaatcagc aatctggaaa tctaattctc gagcggtatg ccaagccagc caagtcattg
    34081 atttgatctg cggggctcta tcttccactg gaaaacgatt ggcctcctca atagagacgc
    34141 cttcaaacaa acgttcaaac cgatcgtatg cccgatccac actctcgcat agcatatcta
    34201 aataatccat ttattttcct cctttttatt tatcctactt actttttcct aattctagga
    34261 tacctactta tatgttacta cacaccctta ttttttacac tttaacagtc accatcatca
    34321 atagtttgga gattgaaaga aaaagatagt gtcgaactat caattgaaca agaagcaaca
    34381 atatgaacgt ttcattaata ttctttaaat aaacaatgat ttttcaaacg aacagtttaa
    34441 gactaggaga aaaagagtat aataggaaaa tgtttgaaag gaaaggaagg ggaaaaatga
    34501 aaaaaagggc aagtttacta gtcctagttg ctttgttgtt caatatcttt tcgtatggtg
    34561 tggccgtttc agcggaaaca tacattccta aagaccctgg gaatatttat aatgatctaa
    34621 ctggtaacta tggcttgtta ggaattgcta gtcaattcca cgtatttgct aaaaataaga
    34681 caactgtgaa tgcacataca gacggaaata tagcaacaca tgaacttgat gcaaaaaata
    34741 attttggtac agagcttcat gaaggtttat tgaaccaaga aataaattat atacaaaaaa
    34801 ctgattcttt cgctaattct tcaggcatac ctacaggtag cacaagaatg aataaatttg
    34861 tcgttggcag taacgtggat gtagcacttc aagatggtca tcctacagtc aatggatcca
    34921 ggcttgatca cttaactcca gatgattttt ataaagatcg tgctgggcat gaatatattg
    34981 atttcgagca ggaatttgct aaattgaatg tagcagcaaa tgatcttgca acgatcactc
    35041 cagctaaaac atatactgct gcagattttc cagacatgaa caatcggaca atcgatttaa
    35101 ctgatttatc agataccggg tttttattgg taaatatcga tgcagaagtg ttaacgatga
    35161 atacgccact tcaaatcatt aatccaaatg atcaagtagt tgtatttaat gtcatcaaca
    35221 gttcttctgc attgaacgta cagtcaccaa tcaaatacaa tgatcgttca aatcatgaaa
    35281 cagaagattt ttcggatgcg aatatctctt ggaattttgg aaatgagatg actgatttga
    35341 caatcagtgc tccttttcaa ggaacaatcc ttgcgcctaa tgctactatc cgcattactc
    35401 aaaaccaaga cggaacgatt ataggaacaa atgtgatttt agatgcagct accaatcgtt
    35461 gggatcctaa cgaaatattt atcagcgaca atggtacaga tacgacagac acaagcgaca
    35521 gtacagatac atctaccaca agtgatagct cagattcatc aacttcaagc gacagtacag
    35581 atacta
//
LOCUS       DAZKCZ010000018        34477 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000018 DAZKCZ010000000
VERSION     DAZKCZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.018"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8KN_000741"
     CDS             212..604
                     /locus_tag="ACG8KN_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9187823.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8KN_000742"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8KN_000742"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFP9187824.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8KN_000743"
     CDS             2698..2943
                     /locus_tag="ACG8KN_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187825.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8KN_000744"
     CDS             3165..4775
                     /locus_tag="ACG8KN_000744"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFP9187826.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8KN_000745"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8KN_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187827.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8KN_000746"
     CDS             5067..5936
                     /locus_tag="ACG8KN_000746"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9187828.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8KN_000747"
     CDS             6101..7372
                     /locus_tag="ACG8KN_000747"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9187829.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8KN_000748"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8KN_000748"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9187830.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8KN_000749"
     CDS             8763..9026
                     /locus_tag="ACG8KN_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9187831.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8KN_000750"
     CDS             9471..9920
                     /locus_tag="ACG8KN_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9187832.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8KN_000751"
     CDS             9977..11395
                     /locus_tag="ACG8KN_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9187833.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8KN_000752"
     CDS             11395..12255
                     /locus_tag="ACG8KN_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9187834.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8KN_000753"
     CDS             12429..13202
                     /locus_tag="ACG8KN_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9187835.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8KN_000754"
     CDS             13349..13714
                     /locus_tag="ACG8KN_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFP9187836.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8KN_000755"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8KN_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9187837.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8KN_000756"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8KN_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187838.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8KN_000757"
     CDS             15560..16216
                     /locus_tag="ACG8KN_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9187839.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8KN_000758"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8KN_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFP9187840.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8KN_000759"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8KN_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFP9187841.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8KN_000760"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8KN_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9187842.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8KN_000761"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8KN_000761"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9187843.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8KN_000762"
     CDS             21888..22202
                     /locus_tag="ACG8KN_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9187844.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8KN_000763"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8KN_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9187845.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8KN_000764"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8KN_000764"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9187846.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8KN_000765"
     CDS             23546..23875
                     /locus_tag="ACG8KN_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFP9187847.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8KN_000766"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8KN_000766"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9187848.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8KN_000767"
     CDS             24863..25687
                     /locus_tag="ACG8KN_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFP9187849.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8KN_000768"
     CDS             25680..26027
                     /locus_tag="ACG8KN_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFP9187850.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8KN_000769"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8KN_000769"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9187851.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8KN_000770"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8KN_000770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9187852.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8KN_000771"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8KN_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFP9187853.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8KN_000772"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8KN_000772"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9187854.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8KN_000773"
     CDS             31684..32451
                     /locus_tag="ACG8KN_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187855.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..>34477
                     /locus_tag="ACG8KN_000774"
     CDS             32441..>34477
                     /locus_tag="ACG8KN_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9187856.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIE"
BASE COUNT        11076 a         6118 c         7242 g        10041 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggtccag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgattt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgag
//
LOCUS       DAZKCZ010000019        34174 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000019 DAZKCZ010000000
VERSION     DAZKCZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.019"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..1360)
                     /locus_tag="ACG8KN_000775"
     CDS             complement(65..1360)
                     /locus_tag="ACG8KN_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9187857.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            1674..1904
                     /locus_tag="ACG8KN_000776"
     CDS             1674..1904
                     /locus_tag="ACG8KN_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFP9187858.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            2001..3302
                     /gene="murA"
                     /locus_tag="ACG8KN_000777"
     CDS             2001..3302
                     /gene="murA"
                     /locus_tag="ACG8KN_000777"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9187859.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            3315..3491
                     /locus_tag="ACG8KN_000778"
     CDS             3315..3491
                     /locus_tag="ACG8KN_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9187860.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            3626..3922
                     /gene="yidD"
                     /locus_tag="ACG8KN_000779"
     CDS             3626..3922
                     /gene="yidD"
                     /locus_tag="ACG8KN_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9187861.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            4107..4574
                     /locus_tag="ACG8KN_000780"
     CDS             4107..4574
                     /locus_tag="ACG8KN_000780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9187862.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            4659..5543
                     /locus_tag="ACG8KN_000781"
     CDS             4659..5543
                     /locus_tag="ACG8KN_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9187863.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            5657..5893
                     /locus_tag="ACG8KN_000782"
     CDS             5657..5893
                     /locus_tag="ACG8KN_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFP9187864.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            5981..6061
                     /locus_tag="ACG8KN_000783"
     tRNA            5981..6061
                     /locus_tag="ACG8KN_000783"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6015..6017,aa:Tyr,seq:gta)
     gene            6068..6139
                     /locus_tag="ACG8KN_000784"
     tRNA            6068..6139
                     /locus_tag="ACG8KN_000784"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6100..6102,aa:Gln,seq:ttg)
     gene            6264..6434
                     /locus_tag="ACG8KN_000785"
     CDS             6264..6434
                     /locus_tag="ACG8KN_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187865.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            6654..7361
                     /locus_tag="ACG8KN_000786"
     CDS             6654..7361
                     /locus_tag="ACG8KN_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9187866.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(7420..8064)
                     /locus_tag="ACG8KN_000787"
     CDS             complement(7420..8064)
                     /locus_tag="ACG8KN_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFP9187867.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            8190..8477
                     /locus_tag="ACG8KN_000788"
     CDS             8190..8477
                     /locus_tag="ACG8KN_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187868.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            8585..8905
                     /locus_tag="ACG8KN_000789"
     CDS             8585..8905
                     /locus_tag="ACG8KN_000789"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9187869.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            8986..10299
                     /locus_tag="ACG8KN_000790"
     CDS             8986..10299
                     /locus_tag="ACG8KN_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9187870.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            10296..11015
                     /locus_tag="ACG8KN_000791"
     CDS             10296..11015
                     /locus_tag="ACG8KN_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFP9187871.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            11143..11694
                     /gene="raiA"
                     /locus_tag="ACG8KN_000792"
     CDS             11143..11694
                     /gene="raiA"
                     /locus_tag="ACG8KN_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9187872.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            11916..14450
                     /gene="secA"
                     /locus_tag="ACG8KN_000793"
     CDS             11916..14450
                     /gene="secA"
                     /locus_tag="ACG8KN_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9187873.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            14576..15677
                     /gene="prfB"
                     /locus_tag="ACG8KN_000794"
     CDS             join(14576..14647,14649..15677)
                     /gene="prfB"
                     /locus_tag="ACG8KN_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9187874.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            15782..16468
                     /gene="ftsE"
                     /locus_tag="ACG8KN_000795"
     CDS             15782..16468
                     /gene="ftsE"
                     /locus_tag="ACG8KN_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9187875.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            16461..17345
                     /gene="ftsX"
                     /locus_tag="ACG8KN_000796"
     CDS             16461..17345
                     /gene="ftsX"
                     /locus_tag="ACG8KN_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9187876.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            17497..18945
                     /gene="cls"
                     /locus_tag="ACG8KN_000797"
     CDS             17497..18945
                     /gene="cls"
                     /locus_tag="ACG8KN_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9187877.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            19282..20139
                     /locus_tag="ACG8KN_000798"
     CDS             19282..20139
                     /locus_tag="ACG8KN_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9187878.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            20254..21174
                     /gene="pstC"
                     /locus_tag="ACG8KN_000799"
     CDS             20254..21174
                     /gene="pstC"
                     /locus_tag="ACG8KN_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFP9187879.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            21174..22058
                     /gene="pstA"
                     /locus_tag="ACG8KN_000800"
     CDS             21174..22058
                     /gene="pstA"
                     /locus_tag="ACG8KN_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9187880.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            22073..22879
                     /gene="pstB"
                     /locus_tag="ACG8KN_000801"
     CDS             22073..22879
                     /gene="pstB"
                     /locus_tag="ACG8KN_000801"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9187881.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            22895..23653
                     /gene="pstB"
                     /locus_tag="ACG8KN_000802"
     CDS             22895..23653
                     /gene="pstB"
                     /locus_tag="ACG8KN_000802"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9187882.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            23666..24343
                     /gene="phoU"
                     /locus_tag="ACG8KN_000803"
     CDS             23666..24343
                     /gene="phoU"
                     /locus_tag="ACG8KN_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9187883.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            24919..25611
                     /locus_tag="ACG8KN_000804"
     CDS             24919..25611
                     /locus_tag="ACG8KN_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9187884.1"
                     /translation="MLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            25608..26375
                     /locus_tag="ACG8KN_000805"
     CDS             25608..26375
                     /locus_tag="ACG8KN_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9187885.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            26475..28043
                     /gene="liaX"
                     /locus_tag="ACG8KN_000806"
     CDS             26475..28043
                     /gene="liaX"
                     /locus_tag="ACG8KN_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFP9187886.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            28340..28660
                     /locus_tag="ACG8KN_000807"
     CDS             28340..28660
                     /locus_tag="ACG8KN_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFP9187887.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            28660..29010
                     /locus_tag="ACG8KN_000808"
     CDS             28660..29010
                     /locus_tag="ACG8KN_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9187888.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            29177..30115
                     /gene="hprK"
                     /locus_tag="ACG8KN_000809"
     CDS             29177..30115
                     /gene="hprK"
                     /locus_tag="ACG8KN_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFP9187889.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            30130..30960
                     /gene="lgt"
                     /locus_tag="ACG8KN_000810"
     CDS             30130..30960
                     /gene="lgt"
                     /locus_tag="ACG8KN_000810"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9187890.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            31004..32029
                     /locus_tag="ACG8KN_000811"
     CDS             31004..32029
                     /locus_tag="ACG8KN_000811"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9187891.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            32044..32982
                     /gene="galU"
                     /locus_tag="ACG8KN_000812"
     CDS             32044..32982
                     /gene="galU"
                     /locus_tag="ACG8KN_000812"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9187892.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            33107..33547
                     /locus_tag="ACG8KN_000813"
     CDS             33107..33547
                     /locus_tag="ACG8KN_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFP9187893.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            33550..34152
                     /locus_tag="ACG8KN_000814"
     CDS             33550..34152
                     /locus_tag="ACG8KN_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9187894.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIIIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT        11304 a         5819 c         7379 g         9672 t
ORIGIN      
        1 ggtaaagaga aatcttagaa taatttggac agaacagaaa gaagaaaacc tctttctgtt
       61 ctgtttattt ggttaaaaat gttttgccat gacgaatcat aattcgtagt ttgaaattgt
      121 aaaagttgcg aaagccgtac gcattgcgtt ttaatacttt gatgtggttg tttaagcatt
      181 ccaatggtcc gtttgaatag ctgtagtgaa gcgcattttg gatctgtggc agaaactttt
      241 taaacgttcg taaagtggtt gtataggttt ctggcagacc gctaatgtct ctagttaaac
      301 aatcctcaaa tagtgactga tctcttttct ttaccgcata aagaatgtct tggtataact
      361 gatagccttg gcgtaagttg ggacaagcag ctaacagtcg atcggcaatc tcactttcag
      421 tcaaataatc tttaaaactg gcacgccaaa cccgtttttc atagtttaac tttgcttctt
      481 ctttttgtag gagtttccaa tagcgcttca attttttccc taaccctcgt tgagagtggg
      541 aatttccttt aagtcgtacc gtggtttctt tcactcgatg attcaagaaa gttctgccaa
      601 tatgttggac gatgtggaaa cgatcgatga tcaactgagc attcgggaaa agctttttca
      661 ccaacgaaac ataaggcgca tacatatcaa taacaatcaa ttgaacggct tcacgagtcg
      721 ctaatgaaaa ccgagaaaaa tagcgttcta aataaggcaa gcgtcgattt tcgacaatat
      781 ccaataatgc atggctttgt ccgtccatca tgataaaact cattgcactg gcaacacttc
      841 gaaccgattt gaattcatca aagcataaga cctttggcaa agtctgtttg aaaggattca
      901 atgggcggta gaatcgcttc atgacacgat agaccgaaga agtggaaata tttttctgac
      961 gagcgatttc cgtcatggaa gtattctctt ttaagcgagc aatgacagac caacgaactt
     1021 tttgactaat gaaacagtgt ttttcagcaa cagaggtatc cgctacgaat gttctttggc
     1081 aagacttaca tttaaatcgt tgtttcttca gctccagata agtgcgatac tcagacacat
     1141 cattcagtaa caaccgaacg gttgtcgtcc cccaacgaat gatcctagag gggacgacac
     1201 aaagacaatg ggaacactct tctggcttgt acagaagagt tcctttgtaa atcaagcaaa
     1261 tttgcccttt gtatttttct ttagttaaac aattttcctc gaaatagata tttaggtcta
     1321 gaatatttag ggtatctttg ataagctgag tatgagacat cttgaatcct cctatgaatt
     1381 ggtagccaaa ttaattctaa aggatttcag ggtgtctttt ctatattttt ttatacaaaa
     1441 aagatgttgg tgaagttcac caacatcaaa aagtatagag cctttatttg gaataaggga
     1501 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
     1561 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
     1621 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
     1681 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
     1741 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
     1801 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
     1861 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
     1921 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
     1981 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
     2041 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
     2101 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
     2161 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
     2221 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
     2281 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
     2341 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
     2401 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
     2461 tgatcggtaa cacgatttat ttagatttcc ctagtgtagg tgctactcaa aatatcatga
     2521 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
     2581 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
     2641 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
     2701 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
     2761 ttgcagatgc tatctctgaa cataaccgcc cattgatttc taaattgatc gaaatgggtg
     2821 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
     2881 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
     2941 ctatccaatt agttgcagaa ggtacaagtg tagtgactga aacagtgttt gaaaatcgtt
     3001 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
     3061 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
     3121 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
     3181 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
     3241 acgttgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
     3301 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttaa
     3361 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
     3421 tcggtggcgg caatccattt aaagtatttc agccaagttt gtggattcat attcgagact
     3481 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
     3541 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
     3601 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
     3661 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
     3721 ttatatgata aaagcaattc aaacccatgg cgcgctaaaa gggacggtaa tggggactgc
     3781 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
     3841 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
     3901 tacgggtagc actgagcgat aaagaaacaa tttggaaaat agcttcttat attttgcaaa
     3961 attgtgagtt attgccgaag tgttggattt tgaacaccgt caaaaaaaag gttgtgaaaa
     4021 atacggctag cactgagcga taaagaaaaa gaggaaattt attgtcaaag taacaactcc
     4081 gcaactactt atatggggga caaaaaatga tcgaagaact aaacaagaaa gatttaccat
     4141 ggtccttgtt attagatgca gatcctgata aagaaaaagt tcaagtatat gtttccagag
     4201 ggagtggctg gatatggaaa gaaaaagaaa aaacaatagg tgtactgatc tatgtggcaa
     4261 gggaaaaaga atacgaaatc gtcaatgtag cagtaacccc aacgtatcaa ggaaagggga
     4321 ttggcggaaa attacttgag acagcttttc agaagctttc acaactcgct tccagtcaaa
     4381 ctcagatcat catacgaacc ggcagtacta gcagtgcggc gttacatctc tatcaaaaaa
     4441 tgggctatgt ggagattggt cgagtcaaag actattttat ccatcattac tctgagccca
     4501 tttttgaaaa tggagatcaa ttaagggatc aagtgatttt agcaattcac ttacctaaaa
     4561 atagctttaa ctaataaaat ttcaattcaa gaaaatgctt tcaaatgcta aatagataca
     4621 ttacaatagc tttagcaaac aaaaaggaga atatttttat gggaatatta gtttttgatt
     4681 ttggtggatc ggctgtcaaa tatggttgtt gggatggcaa agagattaaa ggtaagggac
     4741 aatttgcaac gcctgaatct tgggaagaaa tgaaagcgca attatttcaa gtttataaaa
     4801 aaatactcgt ttcttttgaa gtcgaaggaa ttggaatcag ctctcctgga gtagtgaata
     4861 atgagaaaca ggtgattgag ggaatcagtg cgattcctta tattcatgga tttaatattt
     4921 tccgagattt ggaaactctg tttcaacttc cagtaacgat tgaaaatgac gccaactgtg
     4981 ccggaatggc tgaattttat gaaggtgctg cccgcgatta tcaagatgtt gcttttgttg
     5041 tggcaggaac tgggattggc ggggcgttgt tcacgaaagg acaaatcaat aaaggggcac
     5101 atctttacgg tggagaattt gggttgatgt tcttaaatgg cgataagaca ttcagtaaac
     5161 ttggtacagc ggtacagatg gcttggcgtt attgtgaacg caaaggtctc gataaaaatg
     5221 cttataccgg aaaagatgtc tttgaattag cagaaactgg agatgcaata gcaaaagaag
     5281 aagttgagag tttttatacg tatcttacaa agggattatt cagtattcaa ttttcgtttg
     5341 atccagaagt gatcgtatta ggcggcgggg tttctgcaaa agaagggctc attgatgaga
     5401 tcaaaagccg aatgaagaag ctgacagaac aattcgatct gcatgatttc gaaccgcaga
     5461 ttttattatg tgaatacaga aacgatgcta atcttgtagg tgcagcagca aactatctcg
     5521 ctgtacgtca aggggagaat taagagcttt agagaaggtg cagcttgcat aaaagaagtg
     5581 aatgaaatga ataggagatt tttacctaag gaatgtttta tttgctgcag tatgataaaa
     5641 tatctgagag gtgaaaatga tgtcaaaaaa aaataaagat attgaagtgc gtatagaaga
     5701 agtgaaaaaa gatatcaaag gtaagaatta tgaagtcact cagttattta tagggaaaaa
     5761 aatgattggt gagatcttag cctatggacc aaaagaatat gaaattttct ttggtgaaga
     5821 agattttgga aaagaaaaaa gtcttgaaaa tgcgatagaa acagtgattc gacactggaa
     5881 tctgcacgaa taaaaaaata tcaaaaaaac tttcaataaa gggttgcaaa aatctggaaa
     5941 attaggtatt ataactaagt caggttgata caaacctttc ggaggagtag cgaagtggct
     6001 aaacgcgacg gactgtaaat ccgttcctta gggttcagtg gttcgaatcc actctcctcc
     6061 atttcattgg ggtatagcca agcggtaagg caagggactt tgactccctc atgcgttggt
     6121 tcgaatccag ctaccccagt ataccacaat cgtaagctct cgttagttcg agagctcttt
     6181 tttttgcttt gctaccttgt tccttgaaga ttctgctgat ttaagataga gttaagttaa
     6241 ctacaagaat tggagggatc cacatgaaat ggaaagaaaa agtagacgaa gcagctgaaa
     6301 agttatatga tttggtcaaa tcagaaaaat acaatattga agtgaatatt ccgaaaaaag
     6361 gcggaaaagc ggtccgggta aaatcaaaac gcccaacgaa tcatacaaaa aaatggatgg
     6421 ctaaaaaccg ctaataataa caaaagatcc ataagtcggc attttcatcg attttagggt
     6481 cttttgttat tttctaggtt ggacatatct aaaaaattgt ttctgacaat aggagtagga
     6541 agaacgatgt gtgtctaaat cctctgttga tggatagaaa cctttatctt tttcagttgt
     6601 gaatcaggta tataccagtt ataatagaag aaacgaaagg acgggtgtac gttatgtcga
     6661 atcaattacc tgtatatata cagattcatg accaattaaa aaatgaaatc gaacaaggta
     6721 tatggaaagt cggagaccgt cttccttcag aaagagaatt agcagtgaaa ttcaatgtca
     6781 gccgtatgac gttgagacaa gcaattcaaa acttggcaga tgaaggaatc ttagaaagaa
     6841 agatcggttc gggtacatat gttgcacgag aaaaagtaca agaaaaaatg tctggtgcaa
     6901 caagttttac agaaattatg gaatcacaaa atcgagtacc gtctagtcgg acaatctctt
     6961 atttcttgac ttcgccaagc tcaagtgaaa tggaaaaact aaacttgagt aaagatgaca
     7021 cgatcttgcg aatggaacgt attcgtttcg cagatgatgt ccctatttgt tttgaagttg
     7081 ccagtgtgcc acaaaagctg atcgatggct acagcaaagc agagattact gaatcgttgt
     7141 atcgcacgct agaagaaaaa ggcgggaaaa agatcggtcc agcgaatcaa acgattactg
     7201 ctgtgttggc ttctgagaaa attgctgact atttagaggt aaaacgagga gatgcagttc
     7261 ttcgtttgcg ccagatttct tattttgaag acggtacgcc gtttgagtat gtgcgtacgc
     7321 aatacgtagg aaatcgtttt gagttttatt tggaaaaata aaaaacaggt gatccctgat
     7381 tccgtattag aaataaaaga gaatcagaat cacctgtttt tatatatgat cagttaccag
     7441 ttgctcgtga taagaaacag gaccctcttc aaattggact tgtccgttca gcagctcaat
     7501 gatggattct ttaaagtggt cagcactttt ttcatctaca gcacagatga cgctaacttg
     7561 atcagtatat tggatttctt ttataaaaaa atgttggagt tcaagataat tctgtaattt
     7621 tcctagttgc ggatacgtga tcatcacaga gatctcctgt tgcaaggtac cttctacgat
     7681 tccaatgtca gacaatgctt gagagaccgc atgagtgtat gctcgtatta atccgcctgt
     7741 gccgagtttc gttcctccaa aatatcgagt gactactgca gctacattga tcagttgatt
     7801 ttttttgagg acttccaaca tcggtatgcc cgccgttcca cttggttctc catcatcgct
     7861 gcttcgctgg atttcatttt tttctccaat gatgaaggcg ctgcagttat ggttcgcttt
     7921 ccaatgttct ttttttattt cttgaatgaa actttttgct tcgtcctctg attctattcg
     7981 tctaagagaa cagataaagc gtgatttttt gatctcgatc tcactttgtc cattttcttt
     8041 tattgtacga tagcgctcga tcatcttgtc acctcttttt tcattttctt tattcagtat
     8101 aaataaagtt ctaggttgtt tcaacactgt aacggttgcg caattgctgt agtatgcgat
     8161 aataaagaca aaggcaggtg gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa
     8221 ttacgcatta tttgaagaac aatggcggct ctcaagtaac gttgaccttt acccaatttg
     8281 acgaactgct gtttcccagt aacggacttc ccaaaacagc aagggaaagt acagattggt
     8341 gggcaaatga ttataaacac cctgaaaaag gtgcctatgg ctggataaat gcaggctatg
     8401 aagtagttgt catcaatttg gacaaagaat atgtcgtgtt caataaatta gtgaaatcta
     8461 gttggctatt tgattaaata aaccgcgttt agaaaacgtt gacaaatttc tttcttttta
     8521 tataatagag taacagacgc gaagaaaacg tttgcgtagt ttgttttaaa aaggagagaa
     8581 aagtatggct aaaaaaacaa tcatgcttgt atgttcggct ggtatgagca caagtttact
     8641 agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc gactctgata ttttcgctgt
     8701 gtccgcttct gacgcagata acaatcttgc aaataaagac gtggatgtgt tgttattagg
     8761 cccacaagtt cgctttatga aagctgattt tgagaaacgt ctggaaccaa aaggcattcc
     8821 tttagatgtc atcaatatgg ctgattatgg tatgatgaat ggcgaaaaag tgttagatca
     8881 agcaatcacg ctcataggtg aataaataga acagtataac tatcgaacaa gcgatccaaa
     8941 aaatagatcg cttgtttttt cgtatatttt tttagggcga ataatatgaa tcatttttgg
     9001 ggtagaaggc ttctgacgag agatattaaa gcaatagact gtgaggaggt aaatttacca
     9061 aattatgaga agatcgcagc agtggaacgt ttgagcaact ggattctttg tcatcgctgt
     9121 ggaatgagaa cgtccgtcat tgatggacag cttgcagatt acggttattt ttgtacacat
     9181 tgtctatatt tagggcgatg cgacactcaa caagatttat atttgtttga tcagcctgaa
     9241 gcaaaacctc gagaagtcgt ctttttttgg acaggacagc ttactgaaaa acaaacggaa
     9301 atctcaaaga gaatactttg ccattcagaa aaaagacatc atcttatttg ggctgtaaca
     9361 ggtgcaggga aaacagagat gctttatcct attttagtgg aaactttaaa agctggagga
     9421 agagtagcga tttgtacacc gcgagtcgat gtttgtaatg aattgttttt acgctatcgt
     9481 tatgtttttc cagaagaaaa aatcacgttg ctccatggga atacagcaac ggcttatacc
     9541 tttacttctt ttgttatctg tacgattcat cagctatacc gtttttatcg gtcctttgat
     9601 ttaatagtga tcgatgagat cgatgcgttt ccttataacg gggatgcagg attagctcat
     9661 gctgtgcaaa cagcgattaa accagacgga cgattcatct acttaagtgc cacacctgat
     9721 aaaaagttac taaaggagat caagcagacg tttgacatcc acaaacttgc tttacgcttt
     9781 catcggcgtt tgcttcctga acccgtcttg gttttcatga ataattggcg acaaaaatgc
     9841 aagaacaaga agaagataaa atctttggtg cggatcattc atcaattaat taaagaaaac
     9901 catatccttt tattttgtcc aagtatcacg atgatgcatc gtttgaaagc cacacttcaa
     9961 gaggctcttc cgagatatga gatcacagat gtttcttcaa gagacaatca gcgggcggaa
    10021 aaagtccaga aaatgcgaga agcaaaatat gacatactgt taaccacgat gatccttgaa
    10081 cgtggagtca catttgaaag aatatccgtt atcgttttag gagcggatca tccggttttt
    10141 actaaatctt ctttggtaca aatcgcagga agagccgatc gtaaagggcc ttatacgaac
    10201 agtcaagtat attttttcta tgaagaaaaa actgcagcta ttcgaaaagc ttgtcaggaa
    10261 atcaaagaaa tgaatgaaga agggaagaag atactgtgag gtgcagttgc tgcagtaaag
    10321 atatcgtcca aaatttaaca ttcagcgaga ttattggtgt ttcgaaatac agattacctc
    10381 agacttgttc agtttgtcgc gcacaatttc atttattgga aacaagaggc tgttctggct
    10441 gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa gaaagtaaat tctatccccg
    10501 tttactgcag tgactgtcaa gaatggaaga agaaatatcc gacctatgat tttcagcacc
    10561 gtgccttttt tgtgtacgat gaaaatatga aggcctggct caatcaatac aagtttatgg
    10621 gagatatccg actagcaggc acctttgcat ataaatggac gcagataaaa aaagaatacc
    10681 cgacatacat ctattgtcca atacctttat ctgaaaaaag gcgaacagaa agaggattca
    10741 atcaagtaag cgagatgttg aaagcagcaa gaattccttt tcagatgcta ctcaaaagag
    10801 agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga aagattggag atgcctcaac
    10861 cttttgaact tgcagtcacc ttttctaaga tacaaaataa aaaaatattg attattgatg
    10921 atgtctatac aacaggacag actcttttcc acgcagcaga ttgtttgcag atcgcacagc
    10981 cgaaaatcat ccgaactatt tctttggcga gatgaattag atgtggaaat ttgatagcga
    11041 tttaatttgc aaataaccta gagttcgata taatagaatt aagaagaggg aaagagtggt
    11101 ccttctcctt cttgattgtg gtagacgaaa ggggtaattt ttatgtttag atataatgta
    11161 cgcggcgaaa atatcgaagt aactgaagcg attcgcgact acgttgagaa aaaagtaggc
    11221 aaactagaac gctatttcag tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt
    11281 tatacagaaa aaacagcaaa agtggaagta acgatcccat taccttatct tgtattacgt
    11341 gccgaagaaa cttctccaga tttatacgca agtatcgatt tggttgttga caaattggaa
    11401 cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat
    11461 actgcaaatg ctgctgcttt attcaatgaa gaggacgaaa acgaaaatga ttctgaatta
    11521 gatatcgtac gtacgaaacg cctttcattg aaaccaatgg atagtgaaga agcagtcttg
    11581 caaatgaaca tgctgggaca taacttcttt atttttgaag atgcagaaac aaatggtaca
    11641 agtatcgttt accgccgtaa agatggaaaa tacggactga ttgaaacaga ctgatcagcc
    11701 ttacttaaat aacaacgaaa acttgcagca gctactgaca gctgccgcaa gtttttttga
    11761 gtttcctcct cttttagatg aaatatgaat aaattatgaa gttcttactt taatacgctt
    11821 aagatatttc aattatctat aactaatgat aaaatgaaaa gtaaagtcta catagtctgg
    11881 ataatacaca gaaggaaaag gagattacac aacgcatggc caatttgatt cgttcactta
    11941 tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat tgcaaaaaaa gttgaagcat
    12001 acgctgacca aatggcagca ttaactgatg aacaattaca ggcaaaaact gctgaattca
    12061 agggcagata ccaaaaagga gaaacattag atcaattact tccggaagct tttgcagtcg
    12121 tccgagaagc agcaaaacgt gtattaggtt tatatccata ccacgtacaa ttgatgggtg
    12181 gaatcgtgct tcacgatgga aatatccctg aaatgcgtac tggtgaaggt aaaaccttga
    12241 ccgcaacgat gccagtatat ctaaatgctc tgactggcga aggggtacac gtagttaccg
    12301 tcaatgaata tttagcaacc cgtgactcga cggaaatggg cgaactttat aatttcttag
    12361 gattaacagt ggggttgaat atcaactcaa aatcttcgga tgaaaaacgc gaggcttata
    12421 attgtgatat cacttatagt acgaataatg aattaggatt tgactacttg agagacaata
    12481 tggtcgttta ccgccatcaa atggtacaac gtccgctgaa ttatgccatt gtcgatgaag
    12541 ttgactcaat cttgattgat gaagcgagaa cgccgctgat catttcaggg caagcagaaa
    12601 aatccacagc tttatataca agagcagaca acttcgtgaa acgtttgaaa gaagaagaag
    12661 attataaaat cgatgtacaa tcaaaaacaa tcagtttaac cgaagcaggg atcgaaaaag
    12721 cagaagaaac attcggtttg gataacttat acgacattga aaatactgca ttgacccatc
    12781 acttggatca agcattgaga gccaactata tcatgctttt agatatcgat tatgttgtcc
    12841 aagacggaaa agtactgatt gttgaccagt tcactggtcg gatcatggat ggacgccgtt
    12901 attcagatgg acttcaccaa gcaatcgaag ctaaagaagg cgtagagatc gaagacgaaa
    12961 cgaaaaccat ggcgacgatc actttccaga actatttccg tatgtacaag aaacttgcgg
    13021 gaatgactgg tacagctaaa acagaagaag aagaattcag agaaatttat aacatccaag
    13081 tataccagat tccgacaaat cgtccggtta tccgtgatga tcgtccagat ttgctttacc
    13141 caacattaca aagcaagttc cacgctgtcg tgcaagatat caaagatcgc taccgcaaag
    13201 gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc tgagttatta tcagatatgc
    13261 tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa aaatcacttt aaagaagcag
    13321 agatcatcat gaatgctggt caaaaaggtg ctgtgacgat tgctaccaac atggctggtc
    13381 ggggaacaga tatcaagtta ggacttgggg taagagaact tggtggtcta gcagttatcg
    13441 ggacagaacg acatgaatct cgtcgtatcg ataaccagct tcgaggacgt gcaggacgtc
    13501 aaggagaccc tggtatgtca cagttctact tgtcattgga agatgatttg atgaaacgat
    13561 tcggttcaga acgaatcaaa gctttcttag atcggatgaa gatcggtgag gaagatgctg
    13621 ttatccaaag caagatgctg acgaaacaag tagaatctgc acaaaaacgt gtagaaggaa
    13681 ataactatga cacacgtaaa aacgtcttac aatacgatga tgtgatgcgt gaacaacgtg
    13741 aagtaatcta taaacagcga caagaagtaa tcatggaaga aaaaagctta tccaagccac
    13801 taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga tgcgcataca caattagaga
    13861 aagaaaattg gaatttggaa agtatcatcg attttgccgg cagtactctt gtccatgaag
    13921 atagtattac tcttgcagat atcgaaggaa aaacagctca ggagatcaaa gagtatctaa
    13981 acgaacgagc acaagaagta tttaatacca aagcggctca actaaatggt ccagagcaac
    14041 tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga ctctaaatgg acagaccata
    14101 ttgacgcaat ggatcaatta cgtcagtcaa tcggcttacg tgcttatgga cagaacaatc
    14161 cattagtcga atatcaaaca gaaggctata agatgtttga agatatggta ggcgccatcg
    14221 agtatgaagt cactcgaatc tttatgaaag ctgagatccg tcaaaacgta cagcgtgaac
    14281 aggtttctca aggaacagct tctcactcag aagacggaga tgccaaagaa aatagtaata
    14341 ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg aagaaatgat ccttgtcctt
    14401 gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa tgcaaactaa tttctgacag
    14461 caggattggt gagacaaatt ctttttttaa gaattcgtct caccttcttt ctgttatttg
    14521 ctgattctgt tagactagat ataatgaaaa aagtatagtg aaagaaggaa aaaacatgga
    14581 aatatccgaa attcgaaatc tcctgacaga aatgcaggaa aaggtcacaa gtttcaggag
    14641 gtctctttga cttagatcag ctggaagaag acatcgcgga agcagaagca aaaatggctg
    14701 aacctggttt ttgggatgac agcgaagccg cacaaaaagt gatcagtgaa aataacgcta
    14761 ataaagaaac ctatgaccaa ttccattctc ttgccgaaga actagaagaa atcgaattga
    14821 tggtggagat gctgcaagaa gaacctgatg cagcaatgca agaagaagca gaaacacgta
    14881 tcaatgcatt agatgaaaaa atgaagacgt atgaattatc tatgctgtta gacggacctt
    14941 atgatcgaaa caatgcgatc gtcgaattgc atccaggggc tggcggaacg gagtcccaag
    15001 actggggcag catgctttta cgtatgtata cacgttgggc agagcagcat ggctatcaag
    15061 tagaaacact agattatcaa gcaggtgatg aagcagggat caagagtgtg accctgctga
    15121 ttaaaggaca taacgctttt ggttatctaa aatcagaaaa aggtgttcat cgccttgtac
    15181 ggatttcgcc ttttgactca gcgaaacgac gtcatacttc tttttgttcg gtagatgtga
    15241 tgccagagct tgatgaaacg atcgatatag aaatcaatcc agatgatttg aaaatcgata
    15301 cgtaccgagc aagtggtgca ggtggtcagc acatcaataa aacagaatct gctgtacgga
    15361 tcactcactt gcctacagga acagtagtag ctagtcaggc acaacgctca caattgaaaa
    15421 accgtgagca agcgatgggg atgttaaaag ctaagcttta tcaattagaa gtggaaaaga
    15481 aagaacaaga agcagctgca cttcgaggag aacaaaaaga aatcggctgg ggttcacaga
    15541 tccgttctta tgttttccat ccctattcta tggtaaaaga ccatcggaca aacgtagaga
    15601 caggaaatgt ccaagcagtt atggacggtg accttgattt atttattgac ggctatttaa
    15661 aaatgcatct acaataagaa cccattgtta aattgaaata aaattgtaag attttgcaag
    15721 catgttgaaa cattgccatg ctataatagc taaagactga aaaagatatg gagagaacgc
    15781 catgattgaa atgaaggatg taatgaagaa gtactcaaat ggtacgaccg ctatccgcaa
    15841 tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg gttggtccgt ctggagccgg
    15901 taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa gcttcaaaag gaactttgaa
    15961 cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa gttccttatc tacgcagaga
    16021 aatcggcgtg gtgttccaag actataaatt attaccaaga aaaacagtat atgaaaatgt
    16081 tgcgtacgct atgcaagtta tcggcagaaa acctcgagat atcaaaaaac gagtaatgga
    16141 agtgctggac cttgttggtt tgaaacacaa agttcgagtc tttcctagcg aattatccgg
    16201 tggggaacag caacgtgtct ctattgctcg ggcaatcgtg aatacgccta aagtacttat
    16261 cgcggatgaa ccgacaggaa atctggatcc agaaaactca tgggaaatca tgaaattatt
    16321 agatcggatc aatgcccaag gaactactgt cgtgatggct acacataaca gtacgatcgt
    16381 aaatacgatt cggcaccgag taattgctat cgaaaacggc cgcattattc gagatcaagc
    16441 ggaaggggaa tatggatacg atgattagaa cctttttccg ccatttatta gaaagtatca
    16501 agagtttgaa acgtaacggc tggatgacag ttgcttcagt cagtgcggta acgatcacgt
    16561 tgactttagt cggtgtattc atggcagtta tcatgaatgc aactaaatta gctcaggata
    16621 tagaaggaaa cgttgatgta tctgtattcg tagatatcgg tacaaaagac gaagagatga
    16681 aaaagctaga aaaagaattg aatgacttag atcatgttaa aaaagttgaa ttttcaagta
    16741 aagaacagga attgaaaaaa atccaaaacg aaatgggtga ttcctggaaa ttgtttgaag
    16801 gtgacaataa cccgttatat gatgtgtatg tagtaagtgc tactgatcct tcttatacaa
    16861 aacaagtagc agaagaagca gcagatttga agaatgtttc tcgttctgat tacggtggag
    16921 cttcctcaga tcggattttc caaatcgctg gtgcagttcg gacatgggga ttagttgcag
    16981 cggcattgct gctgttcgta gcgatgtttc tcatttcgaa cacgattcgg atcacgattt
    17041 tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg tgcaaaaaat ggatatattc
    17101 gctggccgtt tttccttgaa ggtggctgga tcggtctgat cggtgcgatt cttccgattt
    17161 tgatcatcac atttggttat gcatggtttt tccaattgat caatccatca ttattacgtt
    17221 cccattattc attgattcat ccacaaaata tcgtttggaa aatcaatctt ttaatggtcg
    17281 gcattggtgt gatcattggt tcacttggct cgatcatttc tatgagacgt ttcttgaaat
    17341 tctaatacca caatataaat aaaatgagaa agttggacag tttcgttcca gctttctttt
    17401 tttattggct aggagaatag aaggaagcgg cttttttccc tttactttaa aaaacaactt
    17461 gggtattctt aagttggtag aggaagaggt gaagccgtga aaattatcgt tgacaatttt
    17521 tttacgatcc ttttgattat gaatattttg ctttccttta tcatcgtttt tcgagaaaga
    17581 aaggaaactg ctcaaacatg ggcatggctg ctcgttttga tgttcattcc agttgtagga
    17641 tttattttat atattttttt aggaagaggg atttccaaag agaaaatctt tgatttgaag
    17701 atccaaggga aaatcggtaa gaacctggaa attgaagaag acaaacaggc attgatgcga
    17761 gggctttatc ctcatccgcc tactggacaa gtagatgtga aacaattgat ctatatgctc
    17821 acggtatttg aaagtacgct ctatacgact aaaaatgaaa tcactttatt tacagatggt
    17881 cgtgaaaaat tcgatgcgct gattgaagat atcaagcaag cagaagacca tatccacttc
    17941 cagtattata tttatcgcag tgatgcatta ggtgaagaag ttcgagatgc attgacagag
    18001 gcggcaagac gaggggtaaa agtcagagtc cttcttgatg catggggttc cacacaagtt
    18061 tcttccagct tttttcagaa tttgaaaaaa gcaggcggag aaatcgcatt tttctttcct
    18121 ctatttgttc cgtatattaa tcctcggatc aattatcgca atcatcgtaa aatcgtagtt
    18181 attgatggga aaatcggata tacaggtggg ttcaatgttg gaaatgaata tctaggattg
    18241 gtaaagaaat ttggttattg gcgagacaat catttgcgta tttacggaga agcggtgtat
    18301 atcttgcaaa accgtttttt gatggactgg aattctcagc atacaaaaga agtaggctat
    18361 tctcccaaat tttttccgtc cattcactct actggtgata tagctgttca aatcgtgaca
    18421 agcggaccag ataccgaaca tgaacaaatc aaaatgactt atttgaaaat gatttcgtta
    18481 gccaaacggg agatattgat ccaaacgcct tattacatac cggatggatc gattcatgaa
    18541 gcactgaagt tggctcttct ttcaggcgta aaagttcata tccaaatacc taacaaacca
    18601 gatcatcttc tcgtttactg ggcaacgtat tctttcgctg cagagctgat cgagtatgga
    18661 gcaaggatcg aaacatatga gaatggattt attcatgcaa agacgatgat tatcgatggt
    18721 ggcattgttt cagttggctc agcaaatatc gatgttcgtt catttcgctt ggactttgag
    18781 gtcaatacac tgatttatga tgaacgaatg gcttcacgtg taagaaaagc attttttgaa
    18841 gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg aaaaccgagg aataatcatc
    18901 aagatgaaag aaggattagc aagattgatt tcgccgctcc tttaaaacac aaaaaggaca
    18961 tgagaaccat caacagatgg agatctcgtg tccttttcta acggggccat ttctgagagt
    19021 acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta actttggggt atctcttgtt
    19081 ttttggggaa agcaagaaat aagctaatat atgctattaa aagagacaca tttcaaagcc
    19141 agtatagcat agaaactttt aatgagatag ttttttttac aattatgagg atttattttg
    19201 ttatttaatg attttgattc gttttcatca agtaatgaaa atgataaaat aaacatactt
    19261 taggattaaa ggagatttct aatgaaaaaa cttaaattat tcttaccaat aataggatta
    19321 gctattcttt tatcaggctg ttcaaagtgg attgatcgag gagaatccat tacggctgtt
    19381 ggctcttctg ctttacagcc tttagtagaa acagttgctg aaacttacca gacagaaaat
    19441 cctggaaaat tcatcaatgt acaagggggc ggaagcggaa ctggtttgtc tcaagtacag
    19501 tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg aagaaaaatc ggggatcgat
    19561 gcctcagcat tagtcgatca tcgagtagca gttgttggga tcactcctat tgtgaacaag
    19621 gatattggcg taaaagacat cagtatggaa aacctgaaaa aactatttac aggcgagatc
    19681 aaaaactgga gtgaagtcgg tggaaaagat cagccagtag ttatattgaa ccgtgcttca
    19741 ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg acggagaaac agctgttcag
    19801 gcacaagaac aagattcaag cggaatggtg cgccagatcg ttgccgacac acccggtgca
    19861 atcagctatg ttgctttttc ttatgtttcc gatgatgtag atacgttgaa tgttaacggt
    19921 gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga cgatttgggc atatgagcat
    19981 atgtacacaa gagatcatcc aaaaaaactg acaaaagaat ttttagacta tatcttatct
    20041 gatgaagtac aagataacat cgttggagaa ctaggttata tccctgtttc tcatatgaaa
    20101 gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat ctttacacaa aatttacaaa
    20161 cttagctaaa cttttacatg ttattaaaac caaattgatg ttcaatttgt acactgagtg
    20221 agtatgttaa acctgattaa gggggagtca tctgtggaag atgtgaaaaa agtattatta
    20281 acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat tcatcagttt tttatgtatc
    20341 gcgctgatcg tgttggttgt attatctatt ttttattttg tagctagtaa aggattggcc
    20401 acctttttta ctgataagat caatgtattt gattttctgt ttggtacaga ttggaatcca
    20461 agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat tgcctatgat cacaggttcg
    20521 tttattgtga cctttttatc tgctattgtt gccacaccat ttgccattgg tgcagcagta
    20581 ttcatgacgg agatctctcc taaaaacgga acgaagatct tacagccggt catcgagctg
    20641 cttgtaggta ttccttctgt cgtttatggt tttatcggtt tatcagttat cgtgcctttt
    20701 gtcagaagta tttttggcgg gacagggttt ggtatcttag ctggtacatt tgtgttgttc
    20761 gttatgatct tgccgactgt cacaacgatg actgttgatg cattaaaggc agtaccacgg
    20821 cactatcggg aagcttcatt agcgcttggt gccacaagat ggcaaacaat ctacaaagtc
    20881 gtgctacgag cagccgtgcc cgggatttta acagcagtcg tttttggtat ggctcgtgcg
    20941 tttggtgaag ccttagctat ccaaatggtc atcggtaatg cagcattgat gccaacagga
    21001 cttactactc ctgcttctac attgacgtcc atcttaacaa tgggaatcgg aaatacaatc
    21061 atgggaacag ttgaaaataa tgtgttatgg tcattagcgc tgatcttgct attgatgtca
    21121 ttattcttta atatcgtgat ccgtatgatt gggaagaaag gagagctgaa ataatgaacg
    21181 caaaacgctc agataaaatt gctacaggtg ttttatatgt agtttcgggg atcatcgtat
    21241 tgattttagc agctttacta ctttatatct tagttcgggg aataccgcac atttcatggt
    21301 ccttcttgac cgaaccttcc aaagcctacc aagaaggcgg tggaatcggg atccagctat
    21361 ttaactcgat ttatttgctt ttgatcacga tgatcatcag ttttcctatt tctttaggtg
    21421 cgggtatcta tctttcagaa tatgctggaa aaaactggct gacagatgtg attcgcacgt
    21481 cgattgaaat cttgagctca ttgccttctg tcgttgtcgg tttattcggt ttcttggttt
    21541 tcgttattca attccagtat ggtttctcga ttatttctgg tgcattggct ttgactgtat
    21601 tcaacttgcc tcttttgact agaacagttg aggagtcttt acaagcggtc cattacacac
    21661 aacgagaagc aggattggct ttaggtttgt cacgctggga aacagtgata aaagtggtgg
    21721 ttccagaagc gacaccaggg attttgactg gtgtgatcct aagttctgga agaatttttg
    21781 gtgaagcagc tgcattgatc tatacggctg gacaaagtgc acctgcgtta gattttacga
    21841 actggaatcc attaagtgtt tcaagcccga tcagcatttt ccgtcaagct gaaacattag
    21901 ctgtccatat ttggaagatc aatacagaag gcacgatgcc tgatggtgtt tctgtttcgg
    21961 ctggcgcttc tgccgtgttg atcattgctg ttttactgtt caacttcggc gctcgtgcga
    22021 ttggcaaacg attataccga aaaatgactt cagcctaagg agaagaagca aaatgaaaaa
    22081 atataactgg aatgaaacaa atgtcatcac catgccaaaa gagaaagaag tagcactata
    22141 tacggatgat ctacatgtct ggtatggcga caatgaagca atcaaaggag tggatctgga
    22201 atttgaaaaa aataagatca ctgcactgat tggtccttcc ggctgcggaa aatctacgta
    22261 tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat acgaaaatca ctgggaaaat
    22321 catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat gtttatgaga tgcgcaaacg
    22381 gattggcatg gtttttcaac gtcctaatcc gttcagcaag tcgatttatg acaatattac
    22441 atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa ttagatgaaa tcgttgaaac
    22501 aagcttgaaa caagctgcct tgtgggacca agtaaaagac agcttggaca aaagtgcctt
    22561 ggctttatct ggcgggcaac agcaacgttt atgtattgct cgtgcgatcg cgatgaaacc
    22621 tgatatcttg ctgctagacg agccggcaag tgctttggac cctatttcga caggaacggt
    22681 tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc atcatcgtga cgcataacat
    22741 gcaacaagcg gctcgtatca gcgactatac tgcctttttc tatctaggaa aagtaatgga
    22801 atatgacaaa actaacaaga tatttaccag accaaaaatt caggcaaccg aagattatgt
    22861 gtccggtcat tttggttaag acggaggcct caacatgaca aaagaaatta tttcatctaa
    22921 agatcttcat ctgtattatg gaaagaaaga agcactaaaa gggatcgatc taagtttcaa
    22981 tcaaggggaa atcactgcga tgatcggtcc ttctggttgc gggaaatcca cttatctgcg
    23041 ttctttgaac cggatgaatg acctgattcc gaatgtaacg atcaccggga gtgttttata
    23101 taaagacaaa gacatctatg gtccaaaaac agatgttgtt gaactaagaa aagaaatcgg
    23161 catggtattc cagcagccaa acccatttcc tttttctatc tatgaaaatg tcatctatgg
    23221 attgaaatta aaaggggaaa aagataaaaa agtccttgat caagtagttg aagaaagcct
    23281 gaaggcagcg tctgtttggg atgatgtgaa agacaaattg cataaaagtg ctttatcttt
    23341 atccggtggg cagcagcagc gtgtatgtat cgcacgtgtt ttggcagtta atccggaaat
    23401 catcttattg gatgaaccaa caagtgcgct tgatcctgtt tctactggga aaatcgaaag
    23461 tatgctgtta gaattaagag agcggtatac gatgatcatc gtgacccata atatgtctca
    23521 agcttcacgt atttcagata agacggcatt ctttttagat ggtcacttga tcgaatacaa
    23581 tgatacgaag aagatattca tgaatccaga aaaacaagag acagaagatt atatttcagg
    23641 tcgatttgga taaggaggaa acggaatgct acgtacacaa tttgaagaag aattgctgaa
    23701 tctccataat cagttctacg aaatggggat gatggtgagc agcgccgtgc acaagtcagt
    23761 tcgtgcatac atcaaacacg ataaaatctt agcccaagaa gttatcgaaa acgatataaa
    23821 tatcaataac atggaaacac gcttggagaa gaaaagtttt gaaatgatcg cattgcaaca
    23881 gccagttaca actgatttgc gtatgatcat tacggtcatg aaagctagct cggatttaga
    23941 acgtatggcc gatcatgcgg tgtctgttgc taaatccacg attcgcgtaa aagggcagac
    24001 gagaataccg gaaatcgaaa aagagatctc cgatatgtcc gattatgtga aaaaaatggt
    24061 ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa gacgcacgta cgatcgccaa
    24121 tatggatcaa agagtcaatg actatttcaa tcgaatctat aatgcaacaa tcaaaaatat
    24181 gcaggaaaat cctgagacag tcatcagtgg aacagactac ttgaatgttg ccagttatct
    24241 agaacggatc ggagactatg tgacaaacat ctgtgaatgg atcgtctact tggctacagg
    24301 gaaaatcact gagttaaata cgaaccacaa tgagattttc taaactaaag cggtgattct
    24361 gccgtttgtt taggaaaaga ggttgtgaaa tcagcggttg tgaaaaagct gttctgcatc
    24421 aagtaataag aacagtcaaa caaaaatagc tcctcatatt tttcatttgg ctggacttat
    24481 taccgcagat gcaagctttt gaacactgtt tattcgggaa agggtttgtg acaaaatatg
    24541 tcacaaaccc ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc
    24601 atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca agctgcttta
    24661 tatgaagtac ctgtcatcga gatcgaaaaa gtacgaataa aagaggatca tcaattgatt
    24721 acaggaagac tgatgaacac gaatcaaaaa ggagaatcga tttatttatc ggttccttat
    24781 caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct agtccaaatg
    24841 catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt cttcttcaca
    24901 agtatcttca ttggcgccat gttgcttatt ggcggaaaac atggtctatt tgctttagga
    24961 agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac taaaatagaa
    25021 gggatttctc tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata
    25081 ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt
    25141 gtttttttag cattttttat ttgtcttttg acaatgaatc tttggaaaga ccaaggactg
    25201 cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta
    25261 ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa
    25321 gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc gggaaggcaa
    25381 gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg
    25441 tcagttccat cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa
    25501 cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatcg
    25561 atccctatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta
    25621 ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt
    25681 atattctttt aggcttattc atcaatttag ggatcttttt cttgttgtta ttcggttatc
    25741 atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta
    25801 tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga
    25861 tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagca atccaaggtt
    25921 ttacaacgat agaactagaa gaattatctg gcttagataa gactttagcg attgatttta
    25981 gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgcgagtg
    26041 tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc
    26101 aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac
    26161 ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca
    26221 ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc
    26281 ttaccacatt gtccgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct
    26341 ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca
    26401 tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta
    26461 aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc
    26521 tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc
    26581 aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc
    26641 aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg
    26701 aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag
    26761 cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag
    26821 cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt
    26881 tgacagaaga agaattagca cagcgtcaga cgttagaagc agaaatcaaa gagttagaag
    26941 ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc
    27001 gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg
    27061 attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg
    27121 gatcacagtt aggaaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg
    27181 acaacatgga atggaaagat gtcagcttgc gtgtccctgg gattgcgaca acgaaatttg
    27241 agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg
    27301 gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac
    27361 tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg
    27421 ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt
    27481 tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat ggcaacatca
    27541 tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg
    27601 tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc
    27661 gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg
    27721 cgacagcaga aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag
    27781 aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac
    27841 cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc
    27901 tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc
    27961 gtcgtgtttc agatggtgaa atcgcgcaag tgcaactttc aacgacaaca ggaagtatct
    28021 atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa
    28081 gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagg
    28141 ctatctcttg aacaccgttt accaagagat tatgaacttg ccaattatcg ccgagtcaca
    28201 aggaataatt tcaaaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa
    28261 ttttgcaaaa agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat
    28321 agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatccaata aaaatgttgt
    28381 tttgacaggg actttagcag gaataggaga atacatcggg attgatccga ctgttatccg
    28441 tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaatcattt tatacatttt
    28501 attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc
    28561 atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga
    28621 agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt
    28681 ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg
    28741 agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc
    28801 aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg
    28861 tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt
    28921 agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta
    28981 attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt
    29041 ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt
    29101 tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac actctaatag
    29161 aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga aaaaattatc
    29221 tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa ctggggacat
    29281 ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata atcgtttaca
    29341 gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat
    29401 catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa gaggattacc
    29461 agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc
    29521 catctcaact tctcgcttat taggagaatt atccagttat ttagatggtc gccttgcccc
    29581 tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac tgattcaagg
    29641 tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg gtcatcgatt
    29701 gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga tcggagaacc
    29761 gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg atgtaatgaa
    29821 tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg tttatctgga
    29881 agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga ccgttgaaat
    29941 cgcggaagta ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat
    30001 catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg acgcaacgaa
    30061 aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg
    30121 agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg gattcctatc
    30181 tactggtatg cattgatcat cgtatcaggt atcattatcg ccatgtggct tagcagcaga
    30241 gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt gtggggattg
    30301 cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc ccagtatata
    30361 gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta tggcggactg
    30421 attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc tttgtggact
    30481 tttttagata tcgctgcacc aagtgtatta ttagcacaag caatcggtcg ttggggaaat
    30541 ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga aagtctgcat
    30601 ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca accaacattt
    30661 ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt acgaaagaaa
    30721 ccacaccttt tgaaaaaagg tgaagtcctt ttatgttatc taatgtggta tagtttcggg
    30781 cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt gatccgtatt
    30841 tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat ttggcgcaga
    30901 aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatctga
    30961 tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc
    31021 ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg aaaacggaca
    31081 tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca cccaacacac
    31141 gaatcaacgt tatttaccag atcattgtct acccgattcc atccaagcat tcaccaatat
    31201 ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga aagcaatacg
    31261 ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca tccatgcaag
    31321 taaaggatta gaacagggga cacataaaag gatctctgac atcttgttag aggaaatccc
    31381 agtagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag aagaagtagc
    31441 gaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag caaaatatgt
    31501 acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg tgatcggtgt
    31561 agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta ttcacggctt
    31621 agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg agatcagccg
    31681 tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg gagtaggcga
    31741 tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca acttgttagg
    31801 acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa
    31861 tactacccaa gcagcgatgg aactagccca aataatggaa atcgaaatgc ccatcactca
    31921 aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat
    31981 gttacgtgat gggaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg
    32041 tatatgaaag tcaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca
    32101 gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac aatccagttc
    32161 atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac aggaaaagct
    32221 aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa cctaagtgaa
    32281 aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc
    32341 atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag agcctttatt
    32401 ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga agtaccattg
    32461 acacagcaat taatcgatga ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa
    32521 gttcctcatg atgaaacttc aaaatacgga attatcaatc caggagaagt attggaagat
    32581 gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat
    32641 ttagctatta ttggacgtta cttattgact ccagaaattt ttgatgtatt gtccgagcaa
    32701 caaccaggag ccggaaatga aatccaacta actgacgcaa tcgatacatt gaataaaaca
    32761 caacgcgtat ttgcccgtaa attcacaggt aaacgttacg atgtcggtga caaatttggt
    32821 tttatgaaaa caagtattga atatggtcta acacatccag aagtaggtgc acctttacgt
    32881 gagtacatcc tgtctttagg taaaacatta gaagcagaag gaagcaagat cgatcataat
    32941 ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aacccttttt tctaacgagc
    33001 ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt aaaaaacacg
    33061 aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt caggaggaga
    33121 aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt
    33181 gttgttgcaa gtttctaaaa ctttagaaaa agtagatcag actgtagcag aagcaaatac
    33241 gacgatcgaa atcgtcacaa aagacgtaga tcttttgtct agacaagtag aaggcttact
    33301 ggtcaaaagt aatgaactgc tcttggatat caatggaaaa gtcgcaacga tcgatccatt
    33361 gttcacagca gttgctgatt taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa
    33421 catcgcgacg aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg ttgccggaaa
    33481 agtcggtggg acagcgatga aattttttaa aaacaaaaaa catactgaaa cgacaggagg
    33541 aaaataaaaa tggctaaaaa aggtggattt ttattaggtg cagtagtcgg aggcacagcc
    33601 gcagcaatcg ccgcgttatt gttagcacca aaatctggta aagaacttcg ggatgaatta
    33661 gctgcccaag cggatgattt gatggatatg gcttcagatt acacagatat ggcgaaagaa
    33721 aaatcgactg agttaacagg tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca
    33781 ggtgatctag caggtagtgt caaacaaaaa gtaagtgaat cagcagatga agcaggaagt
    33841 gaatctgacg atatcttgtt aaatcttaaa gaacaatcag ctgacttatc taatcgtttc
    33901 aaaaaaacgg cagaagatat gaaaaaacct gcagatgaat taggaagtat tgctaaagat
    33961 acttcagaag atatcataat tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc
    34021 aatgaaggaa tgtcagaagc aaaaccagtt gcagatgact caaaagaaat gatgaaaatg
    34081 gcaaaagaag atgcgaaaga agctaaaaaa gaaataaaag atacaatgac agattcatct
    34141 acaaatcact aaaatcacaa atttatagaa taaa
//
LOCUS       DAZKCZ010000020        33403 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000020 DAZKCZ010000000
VERSION     DAZKCZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.020"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            319..627
                     /gene="rpsJ"
                     /locus_tag="ACG8KN_000815"
     CDS             319..627
                     /gene="rpsJ"
                     /locus_tag="ACG8KN_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9187895.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            658..1287
                     /gene="rplC"
                     /locus_tag="ACG8KN_000816"
     CDS             658..1287
                     /gene="rplC"
                     /locus_tag="ACG8KN_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9187896.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1315..1938
                     /gene="rplD"
                     /locus_tag="ACG8KN_000817"
     CDS             1315..1938
                     /gene="rplD"
                     /locus_tag="ACG8KN_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9187897.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1938..2228
                     /gene="rplW"
                     /locus_tag="ACG8KN_000818"
     CDS             1938..2228
                     /gene="rplW"
                     /locus_tag="ACG8KN_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9187898.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2267..3100
                     /gene="rplB"
                     /locus_tag="ACG8KN_000819"
     CDS             2267..3100
                     /gene="rplB"
                     /locus_tag="ACG8KN_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9187899.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3143..3421
                     /gene="rpsS"
                     /locus_tag="ACG8KN_000820"
     CDS             3143..3421
                     /gene="rpsS"
                     /locus_tag="ACG8KN_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9187900.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3444..3791
                     /gene="rplV"
                     /locus_tag="ACG8KN_000821"
     CDS             3444..3791
                     /gene="rplV"
                     /locus_tag="ACG8KN_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9187901.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3805..4461
                     /gene="rpsC"
                     /locus_tag="ACG8KN_000822"
     CDS             3805..4461
                     /gene="rpsC"
                     /locus_tag="ACG8KN_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9187902.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4464..4898
                     /gene="rplP"
                     /locus_tag="ACG8KN_000823"
     CDS             4464..4898
                     /gene="rplP"
                     /locus_tag="ACG8KN_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9187903.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4888..5076
                     /gene="rpmC"
                     /locus_tag="ACG8KN_000824"
     CDS             4888..5076
                     /gene="rpmC"
                     /locus_tag="ACG8KN_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9187904.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5101..5367
                     /gene="rpsQ"
                     /locus_tag="ACG8KN_000825"
     CDS             5101..5367
                     /gene="rpsQ"
                     /locus_tag="ACG8KN_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9187905.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5425..5793
                     /gene="rplN"
                     /locus_tag="ACG8KN_000826"
     CDS             5425..5793
                     /gene="rplN"
                     /locus_tag="ACG8KN_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9187906.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5830..6138
                     /gene="rplX"
                     /locus_tag="ACG8KN_000827"
     CDS             5830..6138
                     /gene="rplX"
                     /locus_tag="ACG8KN_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9187907.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6166..6705
                     /gene="rplE"
                     /locus_tag="ACG8KN_000828"
     CDS             6166..6705
                     /gene="rplE"
                     /locus_tag="ACG8KN_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9187908.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6724..6909
                     /locus_tag="ACG8KN_000829"
     CDS             6724..6909
                     /locus_tag="ACG8KN_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFP9187909.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6945..7343
                     /gene="rpsH"
                     /locus_tag="ACG8KN_000830"
     CDS             6945..7343
                     /gene="rpsH"
                     /locus_tag="ACG8KN_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9187910.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7375..7911
                     /gene="rplF"
                     /locus_tag="ACG8KN_000831"
     CDS             7375..7911
                     /gene="rplF"
                     /locus_tag="ACG8KN_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9187911.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8072..8428
                     /gene="rplR"
                     /locus_tag="ACG8KN_000832"
     CDS             8072..8428
                     /gene="rplR"
                     /locus_tag="ACG8KN_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9187912.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8449..8949
                     /gene="rpsE"
                     /locus_tag="ACG8KN_000833"
     CDS             8449..8949
                     /gene="rpsE"
                     /locus_tag="ACG8KN_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9187913.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8964..9143
                     /gene="rpmD"
                     /locus_tag="ACG8KN_000834"
     CDS             8964..9143
                     /gene="rpmD"
                     /locus_tag="ACG8KN_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9187914.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9187..9627
                     /gene="rplO"
                     /locus_tag="ACG8KN_000835"
     CDS             9187..9627
                     /gene="rplO"
                     /locus_tag="ACG8KN_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9187915.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9627..10922
                     /gene="secY"
                     /locus_tag="ACG8KN_000836"
     CDS             9627..10922
                     /gene="secY"
                     /locus_tag="ACG8KN_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9187916.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10984..11631
                     /locus_tag="ACG8KN_000837"
     CDS             10984..11631
                     /locus_tag="ACG8KN_000837"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9187917.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11822..12040
                     /gene="infA"
                     /locus_tag="ACG8KN_000838"
     CDS             11822..12040
                     /gene="infA"
                     /locus_tag="ACG8KN_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9187918.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12073..12189
                     /gene="rpmJ"
                     /locus_tag="ACG8KN_000839"
     CDS             12073..12189
                     /gene="rpmJ"
                     /locus_tag="ACG8KN_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9187919.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12207..12572
                     /gene="rpsM"
                     /locus_tag="ACG8KN_000840"
     CDS             12207..12572
                     /gene="rpsM"
                     /locus_tag="ACG8KN_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9187920.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12600..12989
                     /gene="rpsK"
                     /locus_tag="ACG8KN_000841"
     CDS             12600..12989
                     /gene="rpsK"
                     /locus_tag="ACG8KN_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9187921.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13069..14007
                     /locus_tag="ACG8KN_000842"
     CDS             13069..14007
                     /locus_tag="ACG8KN_000842"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9187922.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14035..14415
                     /gene="rplQ"
                     /locus_tag="ACG8KN_000843"
     CDS             14035..14415
                     /gene="rplQ"
                     /locus_tag="ACG8KN_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9187923.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14687..15526
                     /locus_tag="ACG8KN_000844"
     CDS             14687..15526
                     /locus_tag="ACG8KN_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9187924.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15502..16371
                     /locus_tag="ACG8KN_000845"
     CDS             15502..16371
                     /locus_tag="ACG8KN_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9187925.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16361..17158
                     /locus_tag="ACG8KN_000846"
     CDS             16361..17158
                     /locus_tag="ACG8KN_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP9187926.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17361..18107
                     /gene="truA"
                     /locus_tag="ACG8KN_000847"
     CDS             17361..18107
                     /gene="truA"
                     /locus_tag="ACG8KN_000847"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9187927.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18440..21109)
                     /locus_tag="ACG8KN_000848"
     CDS             complement(18440..21109)
                     /locus_tag="ACG8KN_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFP9187928.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(21347..21535)
                     /gene="rpmB"
                     /locus_tag="ACG8KN_000849"
     CDS             complement(21347..21535)
                     /gene="rpmB"
                     /locus_tag="ACG8KN_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9187929.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21805..22167
                     /locus_tag="ACG8KN_000850"
     CDS             21805..22167
                     /locus_tag="ACG8KN_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9187930.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            22219..23901
                     /locus_tag="ACG8KN_000851"
     CDS             22219..23901
                     /locus_tag="ACG8KN_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFP9187931.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            24018..26054
                     /gene="recG"
                     /locus_tag="ACG8KN_000852"
     CDS             24018..26054
                     /gene="recG"
                     /locus_tag="ACG8KN_000852"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9187932.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            26103..27104
                     /gene="plsX"
                     /locus_tag="ACG8KN_000853"
     CDS             26103..27104
                     /gene="plsX"
                     /locus_tag="ACG8KN_000853"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9187933.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            27226..27468
                     /gene="acpP"
                     /locus_tag="ACG8KN_000854"
     CDS             27226..27468
                     /gene="acpP"
                     /locus_tag="ACG8KN_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9187934.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            27736..28740
                     /locus_tag="ACG8KN_000855"
     CDS             27736..28740
                     /locus_tag="ACG8KN_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187935.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            28741..29685
                     /locus_tag="ACG8KN_000856"
     CDS             28741..29685
                     /locus_tag="ACG8KN_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9187936.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            29685..30647
                     /gene="opp4B"
                     /locus_tag="ACG8KN_000857"
     CDS             29685..30647
                     /gene="opp4B"
                     /locus_tag="ACG8KN_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9187937.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            30674..31588
                     /locus_tag="ACG8KN_000858"
     CDS             30674..31588
                     /locus_tag="ACG8KN_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9187938.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            31614..33395
                     /locus_tag="ACG8KN_000859"
     CDS             31614..33395
                     /locus_tag="ACG8KN_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9187939.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
BASE COUNT        11074 a         6052 c         7276 g         9001 t
ORIGIN      
        1 tttattgcag gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa
       61 agagtcagta ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc
      121 cttgctttgt gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc
      181 acagacgtaa tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac
      241 gctttgccac gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata
      301 ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa
      361 caccgtattt tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct
      421 agcgtgtctg gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg
      481 actcataaat acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc
      541 gacattgtga acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct
      601 ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg
      661 accaaaggaa tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa
      721 ttaattccag taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg
      781 gaaacggatg gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca
      841 aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt
      901 gaattcaaaa atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt
      961 ttccaagcag gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg
     1021 gttatcaaac gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt
     1081 cgtcctgggt caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct
     1141 ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca
     1201 gaaagaaatg ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact
     1261 atcaaatcag ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg
     1321 aatgtagcat tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa
     1381 atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg
     1441 tcattaagac aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt
     1501 aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa
     1561 tggcgtggag gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct
     1621 aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac
     1681 ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt
     1741 cttgctaact tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt
     1801 gctgctttat cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt
     1861 gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt
     1921 gaggaggtgc ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa
     1981 aatcaatgct tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca
     2041 aaactttagt aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca
     2101 tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac
     2161 gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg
     2221 ctgaataagc actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa
     2281 gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac
     2341 aacatcaaca cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa
     2401 caacggccgc atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat
     2461 cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc
     2521 aaaccgttca gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt
     2581 agcacctaaa ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa
     2641 agtcggaaat gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga
     2701 aatgaaacct ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact
     2761 tggtaaagaa ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt
     2821 agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat
     2881 cggtaaagct ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat
     2941 gaaccctaat gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc
     3001 accagtttct ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa
     3061 atcagacaaa cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc
     3121 acattttgag aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga
     3181 gcatttaatg aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac
     3241 ttggtcacgc cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga
     3301 tggacgtaaa catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga
     3361 atttgcacct acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta
     3421 atttgagggg aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa
     3481 aaacagttcg cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa
     3541 gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg
     3601 aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa
     3661 acttggtagt aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc
     3721 gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat
     3781 cagaaaaata aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta
     3841 ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta
     3901 cacgaagatt taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca
     3961 acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca
     4021 ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa
     4081 ttaactggta aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca
     4141 aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct
     4201 caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta
     4261 tctggtcgtc taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt
     4321 cctcttcata ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac
     4381 ggaaaactag gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac
     4441 actgagaaag gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat
     4501 tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg
     4561 gtttgcaagc tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg
     4621 caatgactcg ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat
     4681 cttatactgc caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat
     4741 gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag
     4801 tagctcgtga agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa
     4861 aacgtgagga aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact
     4921 gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa
     4981 ttagcaacag gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca
     5041 cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt
     5101 atgactgaag gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg
     5161 gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc
     5221 atgaactatt ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac
     5281 atcgtgagaa ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa
     5341 gtagtcgaag aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca
     5401 aaatctgaaa ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat
     5461 aactcaggcg cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact
     5521 gctaacatcg gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc
     5581 aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca
     5641 gacggttcat atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc
     5701 cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg
     5761 aagatcgttt cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc
     5821 gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa
     5881 aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt
     5941 gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt
     6001 gagatggaag cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct
     6061 ggccgagttg gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt
     6121 gaagttttag ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa
     6181 gaaaaatatc ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt
     6241 atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca
     6301 aacgctaaaa acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca
     6361 atgattacaa aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt
     6421 ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct
     6481 gtttcactac cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt
     6541 ggaaactata ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta
     6601 gtagataaag tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa
     6661 tctcgtgagt tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac
     6721 tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa
     6781 gcttatactc gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt
     6841 tgccgtattt gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct
     6901 agctggtaaa aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc
     6961 caattgcaga ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag
     7021 aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg
     7081 ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat
     7141 ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc
     7201 gtgcttatgt caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca
     7261 tttcgacttc tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg
     7321 aagttatcgc ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc
     7381 cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac
     7441 aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa
     7501 atgaatatcg aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa
     7561 acaattcatg gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt
     7621 ttccaaaaag cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa
     7681 cttgtattga acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact
     7741 gttgaagtac cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc
     7801 gaattagctg caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt
     7861 cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga
     7921 aaaattgcat agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa
     7981 aatacatttt tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata
     8041 gtagtacaat aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac
     8101 acgtcaaaag agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg
     8161 cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg
     8221 tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga
     8281 agcagcacaa gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt
     8341 agtagtcttt gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc
     8401 tgctcgcgaa aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc
     8461 gatccaaaac atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt
     8521 gttaaaggtg gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga
     8581 cacgtagggt ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt
     8641 gaagatgcga agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa
     8701 gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg
     8761 gttgctgctg gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact
     8821 tctaaatctt taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg
     8881 aaacaattga aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta
     8941 atcggataag gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta
     9001 tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca
     9061 gttcagtggt taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc
     9121 tagtggacgt tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc
     9181 aaacctatga aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt
     9241 gttggtcgtg gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa
     9301 aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc
     9361 cgtcgtttac caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat
     9421 cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa
     9481 gctggaatcg tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac
     9541 aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg
     9601 gctggtggat caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa
     9661 aagacattag atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg
     9721 cacatattac tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat
     9781 ttttcaacat gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa
     9841 tgggggtttc accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg
     9901 tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag
     9961 cgactcgata tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat
    10021 tcaatcaata cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga
    10081 tcggtttgat tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag
    10141 aaaaaggtat cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc
    10201 ctgaaagtat cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat
    10261 ggaaatctgc tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg
    10321 taacattttt ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg
    10381 gcgcgcctac aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta
    10441 tctttgctag ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat
    10501 tcggttctga aaactggtac aaagtgatga tggagatctt taactataat acagcacctg
    10561 gtgctacgat ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag
    10621 taaaccctga gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac
    10681 gccctggtaa ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg
    10741 gtgcagtctt ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc
    10801 ttccacaatc aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag
    10861 aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt
    10921 aagaaagcgt gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa
    10981 acaatgaacc tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa
    11041 atcattgatg cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg
    11101 caaaacgaaa cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt
    11161 cctgatgaag taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa
    11221 ggcttcttat tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg
    11281 ctaaaagatt tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc
    11341 ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa
    11401 attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat
    11461 caaagagaag atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat
    11521 agtgaaccaa tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct
    11581 cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc
    11641 ccgtagccga cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata
    11701 tgtcagtccg acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga
    11761 actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg
    11821 cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc
    11881 aatgtttaaa gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat
    11941 tcgtatgcat tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata
    12001 cgatcttaac cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca
    12061 ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta
    12121 attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt
    12181 caaggataat tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg
    12241 ataaacgtgt agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa
    12301 agatcttagc tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac
    12361 aaacagatgc tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg
    12421 aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc
    12481 gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc
    12541 cagctcgcgc tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca
    12601 tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg
    12661 cacatatcca ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg
    12721 cattagcgtg gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt
    12781 ttgccgctca aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga
    12841 ctgttgaagt aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac
    12901 aagctacagg tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg
    12961 cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta
    13021 acgtcattga acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc
    13081 gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa
    13141 cctcttgaaa gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct
    13201 tctttgccag gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct
    13261 accatcaaag gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg
    13321 ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca
    13381 gtaactgctg gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac
    13441 atctgtagtg tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt
    13501 ggttatgttc aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc
    13561 gattcaatct acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga
    13621 caccgtgaag attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg
    13681 ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac
    13741 ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa
    13801 aaaatgttgg aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg
    13861 aaacgtgcag gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc
    13921 aaagtccgta acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta
    13981 ggtttaggtt tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt
    14041 tatcgtaaac taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact
    14101 gatttattaa tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca
    14161 actgcagaaa aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct
    14221 gcggctttcg tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt
    14281 gaatcagctt tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc
    14341 ggatacacac gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc
    14401 ttagaacttg tctaaaatca cactaataac atcactttat agatgattct tttagagtgt
    14461 tatgatgttg gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg
    14521 cttggggagt aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac
    14581 agatatgttt gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt
    14641 tttttgatag tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat
    14701 tgaattagaa aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga
    14761 tgtctccttt acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg
    14821 aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa
    14881 agtgggaaat caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat
    14941 gatttttcaa aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt
    15001 tggtcttgaa aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact
    15061 tgagaaagtt agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca
    15121 aaaacaacgt gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga
    15181 tgaagcaacc agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa
    15241 gatcaaagaa gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc
    15301 taatgccaat cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa
    15361 agaaattttt tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga
    15421 aaaactgaag agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga
    15481 agaaaggatg gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag
    15541 ccgaatacac catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac
    15601 agttatactg cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg
    15661 aatgcgcttg ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg
    15721 gatacggata ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt
    15781 ccagaagcac agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac
    15841 tttggtgtca gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg
    15901 ctggatgaaa gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga
    15961 gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg
    16021 gcaggacttg atccccaggg cagaaaagaa atgatggaga tgttttggcg tcttcataag
    16081 gaacagcaga tcactattgt attagttacg catttaatgg atgatgtagc caattttgca
    16141 gattatgtct atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc
    16201 tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt
    16261 gcagaagaat taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa
    16321 ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt
    16381 tggtcgttat atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt
    16441 agccagtttt tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt
    16501 gttggcattt ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat
    16561 ccgaggcatt cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt
    16621 tacaagtggc ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt
    16681 gatgaacggc ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact
    16741 cacgcttact acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt
    16801 gaaagccgta cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt
    16861 tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt
    16921 tgattttggt gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc
    16981 tttatttgtc agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg
    17041 ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac
    17101 gatggttatc attgcttttg tattattaac tgtgctactg atctttttaa gaggataaac
    17161 gttcagtggg ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcaagc
    17221 agtgtatgac aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt
    17281 atctgaaaag aaagaacgaa aaaattttat agaaaaatca ttattccaga taacctttta
    17341 ggaacaaagg agagaaaaac atggtacgtt acaaagcaat cattgcgtac gacggaacaa
    17401 attttaatgg attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa gtagaaaaaa
    17461 cattacaaaa aatggcaaat ggaaaagaaa tcacggtctt tggttcggga aggacagatg
    17521 ccggagtaca tgcgatcggg caagtaatcc attttgatta tccagaggag cgtccgttag
    17581 aacgaatgcg tttcgcgcta gatacccagt caccagagga tattgctgta agacaagtag
    17641 aaattgtttc agaagacttc cacgcaagat atttggtcaa agaaaagacg tatcaatttc
    17701 gagtggatat agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt tattttcctt
    17761 acccacttga tttggaaaaa attcagcggg cgcttcctga tttattaggg actcacgatt
    17821 tcacctcttt ttgtgcatca ggaagctcga tagaggataa aatacgtacg atctacgagg
    17881 ctaagatgga ggtaaatgaa acaaaagacg aattgttgtt tacatttagg ggaaatggct
    17941 tcttgtataa gatgatacgg atcttagtag gaacgctttt gaaaattggc aatggccgat
    18001 tgcctgaaga tagtatccca gatattattg cgaaaaaaga tcgtaatgca gcaggaccta
    18061 cagcacaccc tgaaggactt tatttgtatg aagtgaaata tgaataagag gttatgatag
    18121 aagtggtcag cttcaagaaa taagaaggta tttacgaaaa ttacttctca aaattttgca
    18181 aaatttcggc ttattttcga agaagctact tctggaacac cgtttaagaa agaggtcgtg
    18241 agcctgtcat ttaactctga gtcacaaaga gcaatttcaa aaaacagctt ctcatctttg
    18301 atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt gttacctatt
    18361 ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg cccttggtgc ggacagtctg
    18421 aacaggttcc atcgtttctt tattttctac gtcttcttct gtgtgtgttt cgtggttgtt
    18481 cttctattgc tggatcaatc gtgccataaa aggggcgatt gcggtcaagg taccagacat
    18541 aaagaataaa caatgcaata cataggacaa aaaggattgc acctatcgta aatccttcag
    18601 gcaagtaact gaaagtgaca gtatgtttac ctgctggaac ttttatcatc aagaatgcgt
    18661 ctttgaaatt gctgaccttg actggtttcc catcaatttt agctttccat cctttgtcat
    18721 aaggaatagt tgtcaccaac atggaatctt cagcggtatt tacagtccca gaagcttttc
    18781 tgccagtggc ttccatttcg acaccttttt ctttgatctg attcattgcg gcttgatagg
    18841 ctacaacatc tagaccaacg actttaggct ccatgaaact tatttctttc gtcccataaa
    18901 aacttgcagt aaatgtcact gttgtctctt gaggataata gccaagatca tagtattgac
    18961 cagaaatgtt gatttgcgtt tttcgcgatg taccattgac agtgatcgtc gcactagaac
    19021 tttctaattg cgcaaaattg gttgggaaca aacttaagta agcttgtgta tttgcaggca
    19081 catggacttc ccaagtgagc tcttttggct ggttatctgc tgtttctgag taagtgacgc
    19141 cagtcgttgt ctttgtgatc gtcgtattca actgattctt caaagtaggt gtatagaatg
    19201 taaaatactg ttcatttaga ccagacaatg tattgatcaa gtttgtctga ctggtcaaat
    19261 tatctgtcac tggctgctca acatcattaa cggacaacgg tgcagtaaat gctagtggca
    19321 atgcattgac attctgatac agtgtatagt cgccgctttg accagcttgg cggaagccat
    19381 atttactgaa cgtgctttcg tttttagaaa tgttatattt gattcctgta aacccatcca
    19441 tcagcaaagt attattgtta taacggatat tcaaatttgt ccctcttgaa cggaaaccta
    19501 atttatccag gtaagaggaa gaattacgat ttcttataga agaaaacaaa ctgataccac
    19561 tgtaaccata gttgatcgaa tcgtttgcag atactggatc cagattttcc aagcggtaaa
    19621 acgagtcgga atcttttttt gtttcattta caagagcttt caagtcagga taaggatcag
    19681 tatataaact gcgggaagca taattccaat catcaagaat gccatggatc atactgttcg
    19741 tattgaccag tgcttcagca gacatgatca ataataacaa tatactcaga tgattgaatg
    19801 aaagtttttt atgctgataa aatcccacag caaaagcata aatccctaag aaaaccactg
    19861 tcaatacaaa ggaagtcatc gtcacatagg tgtagcttcc ttcaggtttg atgccccatg
    19921 ccaccgcaaa gagagcagct acgaccaaaa tgattcctaa caacagccca ccatcgtctt
    19981 gttctatctt ttcccatcct tttcctgcaa gcaggagcac taagaaagag aacagatagg
    20041 cataacggaa taaaaacata ttcggtgcat gcataccatg ccagaacaaa ttcaatggaa
    20101 caagataaaa acttgcgata agtatggcga aaagactgcc gtacaaaaat ttatttttca
    20161 acggtacttt ccgactggca aaataaaaca gacagaggac taatggtagc agacctgcgt
    20221 aaacgaacgg aatcgatccg tattttgtcg tgtcataaac accaatcata tttttcatga
    20281 ttagatccag ataagaagtc gcttctgttt tcaacgtagt gatctgactc agtgcctcgc
    20341 catttgaccg aagatccagc actgctggca atacaatgat cattgaagcg ccgcctgcca
    20401 gtaaagaagt gataccataa ggaacaatcg tttttttata ttctgaccag tttgtcaata
    20461 gacgggcgat aaaatataag aaagaaaata cgccaatcat aaaacccata taaaaactgg
    20521 ataaaaacaa gagtaaataa ctgataaata aaagtttcgg ccgtttaaga tccataacac
    20581 gatgaatccc caaaatcact aatggaagat aaacaaatgc atccagccac atgatgatct
    20641 ccgaatgtgc agtgataaat gagatcaatg cgtaactgac gctaagtgca acatgccccc
    20701 agcgaggaat cttgaacgta cgtgcaagaa accaaaaagc caatccggca cagccgacct
    20761 taagcaaagt cagaacatac agtgcatctg gcatcatctg gtttggaaaa aagaacacca
    20821 gtggcgtgaa gatcccgcct agatagtaag aaattaaaga taaataattc aatcctagag
    20881 aagcattcca tgtataaaaa atactttgct ttccaagaag aacatttcgg aagctggcat
    20941 gaaaattaga aaactgtgaa aaggcatcac ttgccaaaat acttctgctg cttccaggat
    21001 agattccgat acttagataa acaagtgcta aaatcaaaaa cggaatcagg aaactagctg
    21061 ctgcgtagcg tccgttttct ctaaagaatg actttaaatt ttttttcata tttattttcc
    21121 accaaatttc tatgaagttg tattcagttt atcatatcct tgtttttctc tgcttaaaaa
    21181 gagtctagtt ttcacaaaaa aataaaattt agaataaata acttcataat ttgttcgtat
    21241 aacaaaaata aacgcaaaaa aagggtagga atcaaaagtc ccacccttaa aaatttttat
    21301 taaataaaaa accgttcact atctgaacgg tccaagtatc attcaattaa acgcgttcaa
    21361 cttttcctga tttcaaagca cgagttgaca cccaaacttt tttaggtttt ccgtcgacca
    21421 taacgcgaac tttttgtaag ttaggtttaa cagtacgttt tgttgagttc atagcgtgtg
    21481 aacggttgtt tccacttttt gtcttgcgac ctgtaaagta acaaactttt gccatggtaa
    21541 tttcctcctt tgccttgatt tggagcaata gaataattca gctcatacca gactaacata
    21601 tcatatagaa tgggaatttg caagtacatt ttcatagata caattgacaa ttgagaagaa
    21661 taattgtaaa ttaaacaagg ttatacctga aattttgcaa ggtttttgag tactttgggt
    21721 aactgccttt tatttcaagt tgcgctatga taaaatatga tagaagaaaa atgggagttt
    21781 tttacccgtt aaggaggctc attcatggct gtaaaaattc aaacgtcagc tggaaccatt
    21841 gaaattacca atgatgtaat tgcaacagtt gttggtggcg ccgcgacaga tgtgttcggt
    21901 gttgtaggaa tggccagcaa aaatcaaatt aaagataata ttactgagat tttgcgtaaa
    21961 gaaaattact caagaggagt agttgtccgc caagaagata atggtattgc tgtagatgtt
    22021 tatacgatta tcagctatgg tacgaaaatc tctgaagttt cccgcaacgt ccaagaaaaa
    22081 gtgaaataca atctagaggc attattaggc gtaactgcca attcagttaa tgtctttgta
    22141 caaggtgttc gtgtactgcc agactaggca ggagactggc tggtaactca gcagtcgatc
    22201 tgaaggagga ttatctaggt gagtgtaaca gaaataagtg caagccagtt ccaggaaatg
    22261 gtccaggcag gtgcgaatcg tctgcaagta aatgcagaat acgtcaattc attaaatgta
    22321 ttccctgtgc cagatggcga tacgggaaca aatatgaatc tatctatgac tagtggagct
    22381 aaggcagtag tagattcaac ctcagaaaaa gtaggagaat tagcagcaat gctttcaaaa
    22441 ggattgctga tgggtgcgcg cggaaattcc ggcgttattt tgtctcaatt gtttcgtgga
    22501 ttttctaagc aaattccaga tgttgtctca ttaaatgctt ctgatcttgc tgcagccttc
    22561 actcatggag ttgaaactgc gtataaagca gtaatgaaac cagtagaagg aacgattttg
    22621 acagtagcta gagaagcagc gaaagctgga gaaaagaaag caaaaagtac tgatgatgtc
    22681 gttgaagtaa tgacagctgt tgtaaaagga gcaaagcgtg ctttagctaa aactccagac
    22741 ttgcttccag tcctaaaaga agtcggtgtc gtagacagtg gagggcaagg actgctgttt
    22801 gtttatgagg gctttttaag tgcattgaat ggagaattcc aagcggatga aacatatgaa
    22861 ccttctccag cagaaatgga tgatatggtc aatgcagaac atcatcgcag tgtccaaggt
    22921 caaatggcga cagaagatat ccattttggt tactgtacag aaatcatggt aaaaatcgga
    22981 gaaggaccta cagtcgatag tacattcgat tacgaagaat tccgtaacta tttagatggt
    23041 ataggagatt ctttacttgt ggtcaatgat gacgagatca tcaaagtcca cgtgcataca
    23101 gaaaacccag gagaagtctt gaattatgga caaaaattcg gttcattagt caaaatcaaa
    23161 gtcgacaaca tgcgcttgca gcatgagacg attttggaac acgatgaaga agtgaacgag
    23221 tttgcgaaaa aagaaatacc acgtatcccg tatggtatcg tagcgatcgc tgctggtaaa
    23281 ggcgtccaag agctgtttga aagtttaggc gctaattatg ttatcagtgg tggacaaaca
    23341 atgaatccaa gtacggaaga cattgttaaa gcgattcaag aagtaaacgc agataaagtg
    23401 attgtcttgc caaacaataa gaacatcttc atggcagctg atcaagcagc agaagtagca
    23461 gatatcccag tagcagttgt tccttcaaga acgatttccc aaggattgac agctatgctt
    23521 tcattcaatg atcaagcttc tttagaagaa aacaaagaaa cgatgacaga aatgttgtct
    23581 agcgttgtga gcggtcaagt cactcacgcg atcagagata cctcaattga cggtgtagag
    23641 atcaaagaag gagacttttt gggcatgatc gatggaaaaa tcctgatctc taacccagat
    23701 atcacagcaa cagcgttaag tacactagaa caaatgatca atgaggacac agagatcgta
    23761 acgatcttgg caggtgatga aggaacagtt gaacaagcaa atgaatttgc tgagaaacta
    23821 acagaaaaac atcctgattt ggaaatcgag atccaccaag gagatcagcc tgtctatcct
    23881 tatctatttt ctgctgaata atttctgtgt cagaatatct ttctaacaca gtattcattc
    23941 ataaaaggcc ttcggttagt gatcaggtat gatcgctgac caagtccttt ttttgttaaa
    24001 ggagggagga aaaatttatg cgttctttag atgatccaat cactatgctg gcaggagtcg
    24061 gagaaaaaag agcagctagt cttgccagct tgggcatcca aacgattgaa gacttactga
    24121 cttattatcc ttttcgctac gaagatattc aggaaagaaa tctaaacgag atccaggatc
    24181 aagaaaaggt tactttgaaa ggaatcgtag tctcgcctcc tgtgatgaat cgatttggtt
    24241 ataaaaaaag ccgattgcaa tttcgtatga tgcaagaaca tgatgtgttc aacgtttctt
    24301 tttttaatca accttatcta aaagaaaaag tcattttatc ggaggaaatc gccgtatacg
    24361 gtaaatggga tgcaaagcgt aaagccttga acgggatgaa gattttaggt tctcaaacaa
    24421 tggatgactt ttcgccgata tatcacgtca acaaatcaat tcgtcaaacg acattagttg
    24481 atctaatcaa aaaagggttt caagaatacg gcacattgat tgaagaaaat ctgccaaaca
    24541 accttgtgga gaaatatcga ctgcttgacc gagcttctgc tgtaagaagt atgcattttc
    24601 caaaagacca cgaagaaaac catcaagcca aacgacgtgt cgtttttgaa gagttctttt
    24661 tatttcaaat gcgtcttcaa ggattgaaaa aagcagaaaa agcagaaacg aatggtttgg
    24721 agatccttta tgatgtccaa aaattgaaac aatttactca aaagctacct tttgaactga
    24781 caaatgcaca aaaacgtgta acgaatgaaa tctgtcggga tcttcgcagt ccgcagcata
    24841 tgcagcgact tttgcaggga gatgtaggta gcggaaaaac agtggttgcc gcgattgcgt
    24901 tatacgctac cgttacagca ggtttccaag gtgcgttgat ggtgcctaca gaaatcttgg
    24961 cacaacaaca tatggaaagc ttgcaacagc tgtttgatcc caatgaagta accatcgcat
    25021 tgattacggg gtcaacgaaa gcaaaagaac gacgtgagct ccttgaaaaa ctagagcaag
    25081 gggaaattaa cgtcgtgatc ggcacacatg cattgatcca agatggagtc gaatttcaga
    25141 atttaggtct agttattacg gatgagcagc accgttttgg cgtgaaccag cggaaagtac
    25201 ttcgtgaaaa aggctggcga ccagatgtct tatttatgac agcgactccg attcctagaa
    25261 cattagctat tacagcatat ggtgagatgg atgtatctat cattgatgaa cttccagctg
    25321 gacgtatccc gatcgaaaca cgctgggtaa gaacacctca attggacagt gtactggatt
    25381 ggacgaaaaa agaactggca agaggacatc aaatgtatgt catctgtccg ctgattgaag
    25441 aatcagaagc attagatgta aaaaatgcag tggaaatcta tgaaaaactt cgtgaactat
    25501 ttgcaccaaa atatgaagtt ggcttgttac atggtaaaat gaagaatcag gaaaaagaag
    25561 taatcatgga agccttcaaa gacaaccaga tgcagatatt agtttcgact actgtaattg
    25621 aagtaggggt aaacgtgccg aacgcgaccg tcatgctgat catggatgcg gatcgatttg
    25681 gattggcaca gctgcaccaa cttcgtggac gtgttggacg cggaagtgat gcttcctact
    25741 gtatcttagt tgctaatcct aaaaatgaac taggtgtgga acggatgaaa atcatgacag
    25801 agaccaccaa tggttttgta ctaagcgaaa aagatctgga attacgtggt cctggtgaag
    25861 tatttggctt ccgtcaatct ggtctgcctc aatttgcgat tgctgacttg gtgacggatg
    25921 gaaatgtttt ggaggtagcc agagaagagg ctgcagccct ttggcaaata aaggattggc
    25981 agatgcttcc tgagtatcag cctttagcgc aaagcctcca aatagaaaat caggataatc
    26041 gttatttcga ttagcagact gtgctatact ttatactgat gacggaaaaa ggagtggaaa
    26101 caatgaaaat tgccgtagat gcaatgggcg gagatcacgc acctcaagca attgtagaag
    26161 gcgtggcttt agcgcagcaa gattttccag aagtagaatt cttactttac ggaaaagaag
    26221 cagaaatcaa gaaatactta acaaatgaaa aaaacatcac gattatccat acagacgaaa
    26281 aaatcaatag tgatgatgag ccagtaaaag caattcgacg aaaaaagact gcatcaatgg
    26341 tccttgctgc acaagcagtg aaaaacggtg aagcagatgc tgtattttca gcagggaata
    26401 caggcgcgct actagcagca gggttgttca tcgtaggacg tatcaaaaat atcgaacgcc
    26461 ctggcttgat gtctaccttg ccagtagtag ggaaagaagg tgccggtttc gatatgctgg
    26521 atcttggcgc taacgcagaa aacaaaccag aacatttgtt gcaatacggt attttaggtt
    26581 ctttttatgc acgtaaagtc cgaggtatcg gacgtcctcg tgtcgcactc ttaaacaacg
    26641 gaacagaagc aacaaaaggt agtgaagtga cgaaaaaagc ctatgaactc cttcagaatg
    26701 aaacaagctt gaattttatt ggaaacgtag aagctcgtga gctactcaat ggtgtggctg
    26761 atgtagtggt cacagatgga tttacaggca acgctgtttt gaaatctatt gaaggaacag
    26821 caatgaatat gatgaatcta ttaaaatctg ctattctaaa tgaagggatc aaaggaaaaa
    26881 tgggcgctat gcttttaaaa gacggcttac gttctttgaa agcagaaatg gattattcca
    26941 aacatggcgg tgccgttttg tttggcttga aagcaccagt cattaaaact catggagcaa
    27001 caggccccga tgctgttcgc tatacgatcc gccaaatcca cacaatgctt gagacagatg
    27061 tagttggaca attagtcgaa caatttgaaa aaagcgaaga atagtatttg gatatctgaa
    27121 gcgatccgtt ccattgaaaa aaaggtggac attcctgaaa aatgccagta aaattagcca
    27181 tggtgtaggt aattgacaag tgtatgattc ttggaggtga aaaagttgac tcgcgaagaa
    27241 gtatttaaca aagtagcgaa aattatttca aatcattttg agatagatac cgataaagtg
    27301 acggatgaat tgaacatcaa agatgattta aaagctgatt caattagtat tatggaattc
    27361 gttttagagc ttgaagatga attcggtacg gaaatttcag atgaagacgc tgaacaaatt
    27421 gaaacagtcg gtggcgcagt agattatatc agcagccatc ttaaataatt tcaaataaaa
    27481 gcaaagaagg ataaaaagct gcatgcagct ttttatcctt ctttttttaa tctttttgac
    27541 acgtaaaagt catttttctg ataattctgg cgataaacag aaattatgtt gcaaagtaaa
    27601 gcggttttct atataattat taataattca ttacacaaag ataagaataa cctttgttat
    27661 aaaacgattt attctgtgta atgtcctttt ttattaagaa aaacttccat ttaggaagca
    27721 gagaggggta ttcgattgtc cgataaccaa ttattagaag ttcagaacct acatacagga
    27781 ttccgactca aagatgacta ttacgatgca gtagatgacg tttcatttac tttagacaaa
    27841 aacgagattt tagcaatcgt tggggaatct gggtgcggga aaagtacctt ggctactacg
    27901 attatgggat tacttgatcc taataacaca aaaattacag gtgagatttt atataacgat
    27961 ctgaatttaa tcgacctgaa cgaaacgctc tataataaaa tccgcggaaa tgatatcgga
    28021 atgattttcc aagatccgtt gtctgcatta aatcctctca tgagaatcga agaccagatc
    28081 aaagaaggac tttcctatca cacaaagatg aatgcagaac agcgtcaagc tcgtgcacta
    28141 gaattgctag aacaagtagg gattcctaat cctgcgagag ttggacgcca atatccccat
    28201 gaactatcag gcggtatgag acaacgtgtc atcatcgcaa tcgcaatcgc ctgcaaacct
    28261 ccaatcgtca ttgcagatga accgacgaca gccctagatg taaccatcca agcacagatt
    28321 cttgatttac tgaaagacct tcaggaagaa acaaaaacag ggatcatctt gatcactcat
    28381 gacttaggag tcgtagcaga aatggctgat cgagtggcag tgatgtatgg cggacagttc
    28441 gttgaagtag caggtgtaaa agaactgttt gatcatccta agcatcctta tacacgctct
    28501 ttactgaact ctataccaca agaaggaaat catgatgcag aactccatgt gatcgaaggt
    28561 gtcgttcctt ctctgaaaaa catgcctcgt aaaggttgtc ggtttgcacc acgtattcca
    28621 tggataccag caagcgatca tgaagaagag cctatccttc atgaagtcgc accgaaccac
    28681 ttggtacgtt gctcctgcta taagcatttc cactttagag acgaaaaagg agaggtgtag
    28741 atgacagaat taatcacaat caaagacctg aaagttcatt accctattcg cagtggattc
    28801 ttcaatcgag taacggatca tgtatatgct gtagatggtg tggattttat tatcgaaaaa
    28861 ggaaagacat atggtttagt gggagagtct ggctctggaa aatctactac gggaaaagcc
    28921 gttgtgggat tagaaaaggt aacagccgga caaatcatgt atgaaggaaa agatgtaacg
    28981 aagcgaagca accgcaaaaa aatgggttat aacaaggatg tgcagatgat cttccaagat
    29041 tcaatgtcca gtttgaatcc taaaaagcga gtgttggata tcattgctga acccatccga
    29101 aattttgaac ggttaagcga tcaagaagaa aagaaaaagg taaaaggact gctagatatc
    29161 gttgggatgc cggaagatgc gctatataaa tatcctcatg aattttcagg cggacaacgt
    29221 caacgtctag gagtagcccg tgctgttgct accaatccaa aactgatcgt agccgatgaa
    29281 cctgtatctg ctctggatct atctgttcaa gcgcaagtat tgaatttcat gaaacgaatc
    29341 cagcaagaat acggcctcag ctatttattc atttcacatg atttaggtgt agtaaaacac
    29401 atgtgcgaca atatcgcaat tatgtacaaa ggtcgatttg tagaaatcgg tacaagagaa
    29461 gacatttata atgacccgcg acatatctat acgaaacgtc tgctttcagc tattccgcaa
    29521 atcgatgtag aaaataggga aagccacaaa gaaaatcgac gaagagtaga acaagagtat
    29581 atccaaaacc aaaaagaata ctatgatgcg actggtcgag tctacgattt gcgtacgatc
    29641 acaactacac acaaagtggc gttgaaagat ggaggtgcaa gctaatgtgg aaaacgattc
    29701 tgcgacgtgt tttattgatg atcccgcaag ttattatttt aagtgttctg atcttcttat
    29761 tagcccaagc gatgccgggg gatccattta ccggtttgat caatccaaat caggatccgg
    29821 cagtgatcga acagatgcga caagcagctg gcttgaatga cccatggtat gtccagtact
    29881 tccgttggat cggaaatgct ttgcaaggcg attttggtca aagtttctta tacaaacgct
    29941 ctgttgctga cttgatcggc gaacggatca tcaacacttt atatctatcg atcttgacag
    30001 ttgtccttac ttatttgatt gctgtgcctc tggggatgct tgctggccgg tatcaaaatt
    30061 caatcttgga taagattgtc gtaatctaca acttctttag ttttgctgtt cctttattta
    30121 tcttcggctt gatcatgcta tttgtttttg gttatcgttt aggctggttc ccaacaagtg
    30181 gatcacaaac actaggtttg caaggtggat ttatcactca atggatagac cgtttgaaat
    30241 atattctgtt accatcaatt acccaagcat ttttggcgac tgccgtcaca atccaatatc
    30301 tgcgaagtga agtaatcgat tcgaaatcgc tcgattttgt cagaacagcc cgttccaaag
    30361 gggtaccgac gaataaagta ttcagtcgac atattttccg aaatgcagcg ttgccgatgg
    30421 cttctcaaat ggggtatgaa atcacttcgt tgattgctgg ttctgtagtt atcgaaaaga
    30481 tttttggtta tccgggagtc ggaaaactat ttattgattc catcggccaa cgggactata
    30541 ccgtgatcac atccttagta ttgattttag gaatcgcgac gctagtgggg acattacttt
    30601 cagatatcat catgagtatc gttgacccgc gtattcggat tcaataaaac caatcagaaa
    30661 ggagaatgga tcgatgagtg aaaaaaacaa tacagcagtt caagaaagta ttcctcctat
    30721 ggggatacga atgattgctc gagaatttaa aaaagataaa attgctattt tttcattagg
    30781 tttgctagtg gttttattat tagtgatctt tatcggtgcg atgctgaccg atcaagacaa
    30841 agtgatgtat gttagtatct tcgacaaata cgctgcacca ggaacagttt cgacacaagg
    30901 acaaaagttc attctgggag cagatgaagg cggacgtgat gtattaggac aattgatcat
    30961 aggtgctaga aactctgtta cgatcggatt tgcgatcacg atccttactt cgatcatcgg
    31021 ggttggatta gggatcatct ctggctacta cggagggatc attgacaaca tcttgatgcg
    31081 gatcgttgat tttatcatga ttctaccgat catgctgatc attatcgtct tcgtatcgat
    31141 tatttccaga tacaatgtct ggtcctttat attcatcatg agtgcatttt attgggtagc
    31201 taaagcccga ttattccgaa gcaagacatt atctgaagca agaagagatt atgtcagtgc
    31261 ctcaaaaaca atgggaacca atgattttaa aatcatgttc cgcgaaatca tgccgaatct
    31321 aagttcatta atcattacta atttgacgat gaactttgcg gcaaacatcg gtatcgagac
    31381 aactttgact ttcttaggtt tcggcttgcc ggcaaatgta cctagtttag gtacattgat
    31441 cggttatgca agtaacggag aagtcttagt gaatcggcaa tggatctggt tacccgcatc
    31501 aatccttatt ttagttttga tgttgagtat caattatgtt ggacaagcat tcaagcgttc
    31561 tgcagatgcg cgccaacggt taggctagta tagaagcgaa gggggaaata ggaatgaaga
    31621 aaacgatttt tggatttgca gcgctgatgt ctgctgtcgt tcttgcagca tgcgggggcg
    31681 gaggtacaga tacctctgga aacaactctg gaggcaacgg caataacaaa gaggaattaa
    31741 cttttccaat tgcaacaaaa aataatgatg atgcaatcga agacggcgaa ttagaagctg
    31801 cggtcgcaac ggacagtcag tttaaaggat tgttccagtg ggagttttac caagattcat
    31861 atgatgcagc ttttatggct ccttctcatg aaccgctatt tgcaaacgat gacgatttca
    31921 gaatcacaga cgacggagct gcaacgcttg atctagatga agatacgaaa aaagcgacaa
    31981 tcaaagtaaa agacaacgtc aaatggtcag atggtgaaga tgtaacggcg gacgatgtga
    32041 ttttccctta cgaaatcatt gggaataaag attacactgg tatccgttat gacgacactt
    32101 tccgtaacat cgtaggtatg gaagaatatc atgatggaag tgccgacaca atctccggaa
    32161 tcaaaaaaat agacgacaaa acagttgaaa tcgaatataa agaagtaaac ccaagcatgc
    32221 ttcaatcggg tggtggtatc tggtcatatg cagcaccaaa acatacgtta gaaggtgtag
    32281 caatcaaaga tatggaatca agcgatcaag tacgtaaaaa tccagtgact tttggccctt
    32341 attacatgag taatatcgtt gcaggagaat cagtagaata cttgccaaac gagcactact
    32401 ggaatggtac gcctaaattg aaaaagatca cgatgaaatc tgtaccgatg gcaagtgcaa
    32461 ctgaagcatt gaactctaaa ttatatgata tgattttcca aatgccaaca gatacttacg
    32521 atacctataa agaaattgct ggctatacaa acttaggccg tgaacaaact tcttatactt
    32581 atctaggatt caagttaggt aaatggaatg cagatacaaa gagtgtggaa tacaatccag
    32641 attcaaaaat ggctgataaa tcattacgtc aagcaatggg ctatgcatta gataataatg
    32701 cagtaggtga acgtttctat gcaggtttga gaagcaatgc gacttcattg attcctccag
    32761 tattccagtc ataccatgat gatgagatga aaggattcac acaagatcta gacaaagcga
    32821 aaaaactatt agatgatgct ggctacaaag atgtggatga cgatggttta agagaagaca
    32881 aagatggaaa taaactaaca atcaactttg cttcgatgtc cggtggggaa acagcgcaac
    32941 cattggcaga ttactatatc caacaatgga aagaaattgg tttggatgtc caatacacaa
    33001 caggtcgtct gatcgaattc aattctttct atgataaatt agaaaacgat gatcctgaaa
    33061 ttgatgttta ccaagctgct tggaatacag gaactgatcc aaatccaagt ggactatggg
    33121 gaccaaatgc tgcgttcaac tacactcgtt atgaatcaga agaaaataca aaattgatca
    33181 acgatatcag ttcaagcaaa gcatttgatg aagagtacca aaaagaagca tatcaaaaat
    33241 ggcaacaata tgcatttgaa gaagcatttg cgattccaac gttgttccgt aatgaagtat
    33301 tgccagtcaa cgaccgtgtc aaagactggg attggtctta tgatgtacca aacccatggg
    33361 caaatgtttc tgttacagca gattcacgtt cataatcact aaa
//
LOCUS       DAZKCZ010000021        33213 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000021 DAZKCZ010000000
VERSION     DAZKCZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.021"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1007
                     /locus_tag="ACG8KN_000860"
     CDS             <1..1007
                     /locus_tag="ACG8KN_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9187940.1"
                     /translation="LPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQV
                     RVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTPD
                     SIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDDT
                     LLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTSTE
                     EGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQLP
                     DELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSAL
                     "
     gene            1032..2216
                     /locus_tag="ACG8KN_000861"
     CDS             1032..2216
                     /locus_tag="ACG8KN_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFP9187941.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(2653..4236)
                     /gene="abc-f"
                     /locus_tag="ACG8KN_000862"
     CDS             complement(2653..4236)
                     /gene="abc-f"
                     /locus_tag="ACG8KN_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFP9187942.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(4341..4469)
                     /locus_tag="ACG8KN_000863"
     CDS             complement(4341..4469)
                     /locus_tag="ACG8KN_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187943.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(4814..4957)
                     /locus_tag="ACG8KN_000864"
     CDS             complement(4814..4957)
                     /locus_tag="ACG8KN_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9187944.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(5180..5659)
                     /locus_tag="ACG8KN_000865"
     CDS             complement(5180..5659)
                     /locus_tag="ACG8KN_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9187945.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(5732..6016)
                     /locus_tag="ACG8KN_000866"
     CDS             complement(5732..6016)
                     /locus_tag="ACG8KN_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187946.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(6094..6288)
                     /gene="ssrS"
                     /locus_tag="ACG8KN_000867"
     ncRNA           complement(6094..6288)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8KN_000867"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(6360..7646)
                     /locus_tag="ACG8KN_000868"
     CDS             complement(6360..7646)
                     /locus_tag="ACG8KN_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFP9187947.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7784..8455
                     /locus_tag="ACG8KN_000869"
     CDS             7784..8455
                     /locus_tag="ACG8KN_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFP9187948.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            8421..8909
                     /locus_tag="ACG8KN_000870"
     CDS             8421..8909
                     /locus_tag="ACG8KN_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFP9187949.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            8922..9869
                     /gene="prmA"
                     /locus_tag="ACG8KN_000871"
     CDS             8922..9869
                     /gene="prmA"
                     /locus_tag="ACG8KN_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9187950.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            9870..10634
                     /locus_tag="ACG8KN_000872"
     CDS             9870..10634
                     /locus_tag="ACG8KN_000872"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9187951.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            10867..13080
                     /locus_tag="ACG8KN_000873"
     CDS             10867..13080
                     /locus_tag="ACG8KN_000873"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9187952.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            13091..13537
                     /gene="dtd"
                     /locus_tag="ACG8KN_000874"
     CDS             13091..13537
                     /gene="dtd"
                     /locus_tag="ACG8KN_000874"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9187953.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            13708..14967
                     /locus_tag="ACG8KN_000875"
     CDS             13708..14967
                     /locus_tag="ACG8KN_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFP9187954.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(15056..16003)
                     /locus_tag="ACG8KN_000876"
     CDS             complement(15056..16003)
                     /locus_tag="ACG8KN_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9187955.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFHIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            16397..17689
                     /gene="hisS"
                     /locus_tag="ACG8KN_000877"
     CDS             16397..17689
                     /gene="hisS"
                     /locus_tag="ACG8KN_000877"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9187956.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            17704..19473
                     /gene="aspS"
                     /locus_tag="ACG8KN_000878"
     CDS             17704..19473
                     /gene="aspS"
                     /locus_tag="ACG8KN_000878"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9187957.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            19582..20457
                     /locus_tag="ACG8KN_000879"
     CDS             19582..20457
                     /locus_tag="ACG8KN_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9187958.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            20723..22606
                     /locus_tag="ACG8KN_000880"
     CDS             20723..22606
                     /locus_tag="ACG8KN_000880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9187959.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(22681..22965)
                     /locus_tag="ACG8KN_000881"
     CDS             complement(22681..22965)
                     /locus_tag="ACG8KN_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9187960.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(22955..23689)
                     /locus_tag="ACG8KN_000882"
     CDS             complement(22955..23689)
                     /locus_tag="ACG8KN_000882"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFP9187961.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            23818..24441
                     /locus_tag="ACG8KN_000883"
     CDS             23818..24441
                     /locus_tag="ACG8KN_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP9187962.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            24633..25754
                     /locus_tag="ACG8KN_000884"
     CDS             24633..25754
                     /locus_tag="ACG8KN_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9187963.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            25761..28889
                     /locus_tag="ACG8KN_000885"
     CDS             25761..28889
                     /locus_tag="ACG8KN_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9187964.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            28908..32102
                     /locus_tag="ACG8KN_000886"
     CDS             28908..32102
                     /locus_tag="ACG8KN_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFP9187965.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(32248..32955)
                     /locus_tag="ACG8KN_000887"
     CDS             complement(32248..32955)
                     /locus_tag="ACG8KN_000887"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9187966.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10850 a         5985 c         7067 g         9311 t
ORIGIN      
        1 tgttaccaga aaaaatggaa caagggttta atcaaaacct ggaagctatt cagcttttac
       61 agaatgcatt gggaacttcc tttttagaag cctatgtcga aaatgcagaa aatttaatag
      121 atgattatca agtacgtgtg gtggatggag tgccgacaaa agagacaacg caacggatca
      181 cagctcttta tgaagagttg aagaaacttt ctttcgagcc agaagaatgg cgcagactgt
      241 ctcaattact tttattgaag ggcagccaga cagaacattt gcaggcgaac catcaactca
      301 ctcctgatag tatcggtttt ttatttgttt ttcttattga acaactatac acaaataaaa
      361 aagcaccggt taagattctg gatattgcgg caggaatggg caacttgtta ttaacagttc
      421 ttttaaacct atcgaatgcg gggtaccaaa cagaaggaat tggtgtggat attgatgata
      481 cattgttggc tgtcgcagct tctacaagtg atttgacaca agcaaatgtt caatactttc
      541 atcaagacgg gttgcaggaa ttattaatag atcctgtcga ttttgcaatc agcgatttgc
      601 caatcggtta ttatccgaat gacgaaaaag caaaagagtt tttaacaagt accgaagaag
      661 gccatagtta tgcacatcat ttattgctag agcaatcaat gaaatatgtg aagccggatg
      721 gttttggttt gtttttgatg ccgagtggat ttttagaaac ggatcaaagt gaagaaatca
      781 aaaaatggtt taaagaagaa ggttatcttc aaggaatgat tcaattgcca gatgaattgt
      841 tccgtaataa acaatcgcaa aaaagtattt tgatcttaca gaaaaaagga ccacaagcac
      901 aacaagtaaa agaagtcttg cttgtgaaac ttgcttcatt aaaagaacca gagaaggtga
      961 cggaattttt caacgagttt aaaaactgga aatcatctgc tctgtaatca ttcaacgaga
     1021 aggagagtca tatgtctaaa acaattgcaa tcaatgctgg gagttcaagt ttgaaatggc
     1081 aattatatca aatgccagaa gaaaccgtag ttgcgaaagg gatcgtcgaa cgtatcggtt
     1141 tacaagattc tatttttacc atcaagtacg gcgataatca aaaattcgaa caaatcatgg
     1201 atatcgaaaa ccacgaagta gcagtaaaaa tgctgttaga tcagttgatt gaactgcaaa
     1261 ttttaagctc ttatgatgaa atcaccggag tagggcaccg tgttgttcac ggaggagaaa
     1321 cttatgggga ttcagtagtg atcgatgaga aagtaatgga ccgcattgct gaattagcag
     1381 aatttgctcc tttgcataat cctgcaaact taatggggat caaagcgttt aaaaagattt
     1441 tacctgacat tttgagtgtg gctgtattcg atacatcttt ccactcaaca atgccaaagc
     1501 aaaattattt atatagctta ccggtgtctt attatgaaga tttcaaagta agaaaatatg
     1561 gcttccatgg cacaagtcat cgttatgtgt cggaacgtgc ggcaaaatta ttgaacaaac
     1621 cgatcgaaga actaaaaatc atcacttgcc acttaggaaa tggtgtatcg atcacagcag
     1681 tcgatggagg taaatccatg gatacttcta tgggattcac accattagct ggggtgacaa
     1741 tgggtacacg ttcaggtgat atcgatccag ctgttctccc atatttaatg gaaaaattag
     1801 agattgatat tgatgaaatg atcaatgttt tgaacaagaa atctggtctg ctaggcctaa
     1861 caggattgtc tagtgatatg cgtgatcttg aaaagaacta tgaaaaagaa catattcgtt
     1921 tagcttatga cattttcgtg gaccgcatcc gaaaatacat cggcggttat gtgacaacga
     1981 tgaatggtgt tgatgcaatt gtctttactg ctggtatcgg cgagaatgac ggacatgtac
     2041 gtagtgaaat catcaaaggt atgtcttggt ttggttgtga aatcgatcca gcgttgaatg
     2101 aattgcgtgg tgaagaagta gatatctcta caaaagaatc aaaagtaaga gtattagtga
     2161 tcccaacaga tgaggaacta atgattgccc gtgatgtgga gcgtctgcgc ggctaagcca
     2221 aagcattcgt taggataaaa gatgaaacgc atgcaagagg gaattcttgc atgtgttttt
     2281 tttgcggaga agaccgcaaa atggtaattt aacttcaatt tcccttcttt tttacaggat
     2341 cttcttaggg aattttgctt ggaaaagtgg tcaaaaggga aaagactggc attttattta
     2401 ttgattttca ggaaaatccc aatattttat tttcattcat ctttataagg gacattacat
     2461 atgtaaaaag aaaaaacatt tttagatcag cctcgtgggc tgtcgacttt attcttcgct
     2521 ttatgtttag ctagaaaacc gatccgaaac attcattgat cgaataataa aaacttgatt
     2581 ttccacctgt actttgcctg aatcgtgaaa gcacaggtat gaaaatcaag ttttttgttg
     2641 cgaaaaaacg tcctagtcaa tcagttggcc gagcggctgg cggctgatct gctcctgatc
     2701 aataacgagt gcttcttgga agtgttgctg caaaaagtag cggtcatggc tgactgctag
     2761 gagcgttcct tgatagtctt gcagcatttc ttcaatttct tctcgagcat agctgtcgag
     2821 gtgattggtt ggttcatcca aaatcaggac gttgattttt tctaaaaaga gtttcagcaa
     2881 cgccaaacgg attttttcac caccggataa atctttgatc cgtttaccca catcgctttg
     2941 ataaaagcca cttttggcta attgccgtcg agcgacttct tcgtttccag tcaatgtgat
     3001 ggcgtaagcc agcaaacggc tatcgggatc ggcaaactgc agctgctgcg gcaaataacc
     3061 gatttgcaag ctactaccta atttgatggt gccaccgtaa ggcttttcct caccgagtaa
     3121 taatttgagt aaggttgatt ttcctgcacc attgtcgccg atgatggcta aatgatctcc
     3181 acgatagact gcaaaactgc tgtctttgaa tagtacttga tcagcgtact gctggtgcaa
     3241 gttttgaatg atgaaaactt ctttgccaga ttttccacca ttagacaagc tttgcagccg
     3301 atttttcggg ggaggtggcg gtttgacgag tgtcagtttc gccaggcggc gttccagttc
     3361 tttggctttt ttgaagaagg actcattgtc tccttcatgt gcccactgac gaaaacgacg
     3421 aatcatgact ttcaaccgct ggacttcttt ttgctgcagt tcgtaatttt ttgtcagctc
     3481 agccaagcga gcttctttca agactcgaaa acggctgtag tttccgggat actcaatcaa
     3541 tgcaccttct tccagttccc atgtttttgt ggtgacttga tcgagaaatt gccgatcatg
     3601 agaaacgacc agaaccgatt tttttgtatt ctgtaaatag ttttccaacc agcgaatact
     3661 gttaagatct aaatgattgg tcggttcatc gagtaaaagt aaatcggcat cggctgctaa
     3721 aattttggct aaagcgactt taactcgttc gcctccgcta agttctgtta accgtgcttc
     3781 ctgacggtgt ggaatcccta agccttgcaa gatcgctgca atccggtctt ctaatacgta
     3841 accgcctagt tcttcaaagc gatgctgcag ctcaccgtaa cgggccagac acttctctaa
     3901 gtttgtatct ggttctgtca tcaatgcttc gtagcgactc atttgctgtt tgaggtcctg
     3961 tatctcagcg gcagaatgga gcaagtagtg gttgacggtc gcttcggaaa ctgtaagttc
     4021 ttgttcgacc gtcccgatct ttaagccttt tttgcgactg ataacaccgc tgtcgacacc
     4081 ttctctgccg gtcaagattt gtaaaaaggt tgatttgcca gttccgttga taccgactaa
     4141 ggcaattttt tcaccagctt caatttgaca ggataaatcc tcaaataatg gattcccgcc
     4201 gattgttttt ttaatagaaa ttgcttgtat gatcatgatc tctctccttg agaccactca
     4261 aaaaggccat ggttatccat ggcctgtgtt atttaaactc agattatcat gagaaaaata
     4321 ccttgagatg gccattgatt ttacgtgaat tcaactgttt tggacaaact tgtcccgtca
     4381 acagcaactg taaaaccaat tgcatggcta aatgcgatat ggtatcgttg gtaattttca
     4441 ttacttgtta ttttagtcat gcgcatcata gtattttccc tccttcgtgt agttgtcttt
     4501 tccagtgtac ctgaaagtgt cagaaagttc aactagcgct gacatttact taatatttat
     4561 cgatgtgtaa ttctgtcaga gagctttttt tatctgactg agggtaatgt ctcgtggacc
     4621 acatcattgt attctgagct aaaccagcgt tcaggcgaaa tcatatggtg tttgataaaa
     4681 aactttgtct acatgacttc cttttgccat ttgcttaata ccgttgcatt aaacagctct
     4741 tctctttgga aatctcacca acatcgcgtt caggagtatc gttgtggttg caatggcaag
     4801 cggtagcctt tcatcaattc ttgaatgatt taccgaatgt agcacttgcc ttctcgcggt
     4861 acccgaaccc gaaataaaat tttagtttgg cgataaaatc ggtatccgcc atcgatactt
     4921 catatttttt cgtatcagat tcatcggaaa cttccacatt gacgaaccaa taagctttgg
     4981 catgcttagg tcgttttaaa ccacaccagg gcgactaatc gccctggtgt tcactttttt
     5041 ttattttact tgaactcatt gactgttttc cctttatttt aaacattata aagaccgatg
     5101 agtgcttgcc gcgtcttttg tttttatcac ggttttttgt gcaaaaaaac ctgccgatag
     5161 ttggcaggtc gtctacgtgt taagctttgg tgtggtcttt agctggcaat tggttttcta
     5221 aatcagttcg gacaacaagc acatcgcaag ctgcgtttct aataacatat tcagaaacag
     5281 aaccaataaa taaacgttct acagcattca atcctgtggc tcctaacata atcaaatcta
     5341 cttgattatc ttctgggatt tctctggcaa taatttgttt tggtgagcca tattctacga
     5401 cagaggttac attattcaag ccagctttct tcgcattgct ttcataatct gccaatgttt
     5461 gctttgccat ttcagtagct tgttctgcaa gcattccatc aaaggatgaa acggtttgga
     5521 aagaacgtgt gtcaatgaca tgtgctaata gaagttctcc gttatttcgg atagctacat
     5581 tgaccgcctt tttaaaagct aattcagctt catcagaacc atctacggca accatgattt
     5641 ttttgtattg ttgtaacata agcattcact cctcaagtca atcgtattgt actcttttat
     5701 tgtagaacac tttagggaaa aagagaatta tttactgctt tggttcagca attttttttg
     5761 caaatcggat actgaaggtg gcagaaacaa taattacgat cagtaaaaag accaaggcaa
     5821 taaagcgttg attgaaaaaa gctgttaaaa tacccattac gcctaaaaca gcatatgcag
     5881 ctccgtaaaa ttgtaggaat ccttgatttt tatcattttt gttgatcaat acaaaaaaga
     5941 tatcctgttt attaaataaa taatagctaa cgaataataa tactaaagtg gcgataatca
     6001 ttaaaatcct aatcatgaac aaatcctcca aaataagcaa caaatagaaa aagcaatttg
     6061 ccaagagcaa attgctttat tctttaaaag atggtaatct ccgttaatgc cgttgtttgt
     6121 cctgtagtat tgatcccgcg aattcaagca ggtgggtccc atggtcgtag cttcgaacta
     6181 ccaaatgata aggcatgtta ccagcagtga cacaagacgg gtcccaagat atcaataaaa
     6241 atgttcggtc aacacacaaa gaggacaatt cgaacatcac agatttccca cctctatagt
     6301 agcatatgaa tttaaaaaga gcaatgagag agctcgcaga aacagaaccg ttttcttgtc
     6361 tagttttttg gaggatgctt tttccactct tttatacggt aatactgttg acccagagcc
     6421 tgttcatatt tccctgtatc ttttggctga taatactgag cattcttcag tttatctggt
     6481 aaatattgct gattgaccca agcctgatca aaatgatgag gatactgata acccacgcca
     6541 cggttgagtg atttcgcacc tttgtaatgg ctatctctca aatgatcagg aacatctcca
     6601 gctttgccct ctcgaatatc agcaagtgct gcatctaatg ccatgtaggc agaattggat
     6661 tttggcgaca agcaaagatc gacgacgacg tctgctagtg ggatacgtgc ctcaggcaat
     6721 cccaattttt cagcagctaa tacagcgttc actgtacgag ccgcagcagc aggattacct
     6781 aaacctatat cttcataacc gataaccatc agacgccggc aaatacttgc cagatcacct
     6841 gcctcaacta aacgggcgag ataatgaagt gcagcatcca catcacttcc acgaatcgat
     6901 ttttgaaaag ccgaaataac gtcataatga gcatctccat ttttgtcatg ggtaagagct
     6961 tttctttgga cacattcttc aatgatcgac agtgtaagat gaatccttcc ttctttatct
     7021 gaaggagtcg atagtgtagc cagttccagc ccattcaatg cactccgcaa atcgccgttc
     7081 gttgcacgcg aaaggtgaag aagagcatct tcgtctaact ggattgcttg ttgtccaagg
     7141 ccgcgttctt tatcccttaa agcatgttcg actgccaact ggatgtcttg ttctgttaat
     7201 ggtttgactt caaagatctg tgtacggctg cggatcgctg gattgatggt aatataagga
     7261 ttctcagtag tcgctccgat caagatgatt cggccacttt ctaaatgagg aagcagaaaa
     7321 tcctgttttg ttttatctaa tcggtgtact tcatccaata agaggatcac tgtaccactc
     7381 atttttgctt cttccgctac aacttgtaga tccttttttg aatctgtagc ggcgtttagc
     7441 atgcgaaatg cataattagt agagccggcg atcgcacttg cgatactcgt ttttccagta
     7501 ccaggaggtc catatagaat catggaagag agcatgcgag catcgaccat gcggcgtatg
     7561 atttttcctt ccccgactag atgctgctga ccaacgactt catcgatcgt tcgaggtctc
     7621 atgcgataag ccaatggctg ttgcatagga ttttcctcct tcttgttttt acgtccctta
     7681 attatatcat atttagcaag atgcctagaa agaacagcct tctgttgttt tgttttcata
     7741 gcttatccaa ctaatattgt gtatgataaa gtaggtgaga aaaatgacgg ataaacaaaa
     7801 gatagatgaa tttttttgga ataaaacgac gccagaagtg gcacgggatt tattggggat
     7861 gtatttggag catgacacac cagaaggaag attagctgga tatattgtag acgcggaagc
     7921 atacctaggt ccagaagacg aggccgccca tagctatggt ttaagaagaa caccgcgtgt
     7981 gcgggcaatg tatgaaaaac caggtacgat ctacctttac acgatgcata ctcatcggat
     8041 tttgaacatc attactcagc cagaagggat tcctcaagga gtaatgatac gtgcaatcga
     8101 acccgccgca atgattgatc aaatgagtaa aaatcgtggc ggaaaaactg gtccagatat
     8161 cagcaacggt ccgggaaaat tagtggaagc attagcaatt cctcaagaac tttatggtca
     8221 gtctatagca gatagttcgt tacgcttagt ttttgagaaa aaaaagacac caaaaaagat
     8281 caatgctttg cctagaatcg gtatcccaaa caaaggtgta tggacagaaa agccgttgcg
     8341 ttttgttgtg tcgggaaatc cttatatctc tttacaaaga aaaaaccaaa tcgagaaaga
     8401 ttggggttgg aggaaagaaa atgaaaaaga aggatctatt aacatattta gatgaaataa
     8461 ttgaaaaaca agtgacagac tatgatgtag ctttagattg ggatacaaaa aatcatacga
     8521 tcgagatcgt tgttcgcttg tttgcagaaa ataaagcaca atttgagatt gatgatgcag
     8581 aagggatcgt ttcgcaagaa ccaattatcg aatttgaaga cggcgtactt ctttttaatc
     8641 ctcagaaatc tgtttttgat gaacgcaatt atctagcagt aatcccttat gaaggaaaaa
     8701 aagggttggc aaaatcagta gctgattctt tagtggaata tctaaatgaa gtattagctc
     8761 aagggcaaag cgatttgctt gattttttgg atgaagacga tgacgaagct gtattcgagc
     8821 ttcactggga taatcagcaa ctagcagaat tagtagaagt aaaacagcaa aatgaagcag
     8881 atacttattt accgtaccca agttattaag gagtgacaga tatgaaatgg aatgaagtaa
     8941 aagtagaaac tgaaagtgaa gcagtcgaag cagttgctaa tattttgatg gaggcagggg
     9001 cgagtggtgt cgcgattgaa gattctttag atgttgaaca ttttcagtcg gatcaatatg
     9061 gtgagatttt agataaaaat gattttacat cgctgaaaga aggcgcacta gtcatggctt
     9121 attttccgga aacgactttc ttaccggaaa tcttgccttt tatcaaagat cgtatcacac
     9181 aattacctga gtttggtcta gctattggaa aaaataaagt aacagttagt gaagtagagg
     9241 aaagcaactg ggcaacagcc tggaagaaat actatcatcc ggtgcgtatt acgcgaatgc
     9301 tgacggttgc gccaagctgg gaaaactacc agacagaaga tcctctagaa aaaattatca
     9361 cactagatcc tggtatggca tttggcacag gcactcatcc cacgacttcc ttgacattga
     9421 cagcactaga aacagtcttg cgtggtggtg aaacattgct agatgtggga actgggtcgg
     9481 gcgtgctaag tattgcagca aaatatcttg gtgcaaaaga tgtctatgcc tttgatttag
     9541 acgaagtagc cgttcgttcg gcgaaagaaa atatggatat gaatgaaata gccaaagatg
     9601 tccatgtatc agctaacgat cttttgacag gaatcgagat tgaaagtgat gtaatcgttg
     9661 cgaacatttt agcagatatc atcttattga tgatcccaga tgcttggcgt ttactgaagc
     9721 aaacaggcac gctgatcgtt tcaggaatca ttgaagagaa gaagcaaatg gttctggatg
     9781 ccatgttaga acaagggttt atcgtggatc aaattttcca gcaaaaagac tggtatgcaa
     9841 ttattttgaa aaaaccagag gtggaataga tgcagcggta ttttttaaaa gaatcatatg
     9901 aatcaaaaga ctattttgtt ttagaagggg aaaactatca ccacattgta cgggttatga
     9961 ggatggaacc cgaggatgaa gtttttttgg cattcaatga tcaaatagcc attgttgcag
    10021 aaatcacgga gattactgaa gaggctgttt atctgaaaga aattaaaaaa gaagaaaatc
    10081 aaaaggaact gccaagttcg attaccatag cgagtggcta tcctaaaggg gaaaagctgg
    10141 attggatcgt tcagaaatcc actgaattag gagcgcaagc ttttcttggt ttcccggcat
    10201 cagcctctgt cgttaaatgg gatgcaaaaa aacgtatgaa acgacaacaa cggcttgaaa
    10261 agattgctca agaggcagcg gaacaatccc atcggcagat cgtgccatca gtagagttat
    10321 tggaaacgaa acaagattta tttgagcggc tagatgatta tgacgtggtc ttggttgctt
    10381 atgaggaatc agcaaagcaa ggcgaaaaat caaatgcagc caaaattctt agtgaaacaa
    10441 aaccagatag tcgaatctta gctgtctttg gaccagaagg tggattatcc cgcgatgaag
    10501 tcgattattt tgtagaacac aaagggatac tttgtggatt aggaccaaga atccttcgta
    10561 cagaaacagc acctctttat ttattaagtg ctgctagcta tcaatgggaa ttaatgaatc
    10621 cttctttcag ctaacgttct tcattaataa aagagtatcg acagaaagaa ttgaaaaaat
    10681 agtcaacaat ttgtataata gaaacaatta tttatacaat ggatacgaaa aatcgtttga
    10741 aaaagctttt aaaaagcgaa catttccgta aaaaaacgta taactgcacg gattttggga
    10801 caattatcaa acagatattt gtcccgattc ccgttttacc gaaaaaagaa agggggatcg
    10861 attcctgtga caaaagaaga aattttaacg ggacccggtg tcataaagct cgttagtcaa
    10921 tacatgggtc ccgaacatgt agcatttgtt gagaaagcat gcgaatacgc gactgctgca
    10981 catgacggac agtttagaaa atcaggcgaa ccctacatta ttcatcctat ccaagtcgca
    11041 ggtatattag cagatttaaa aatggatccc catacagtgg ctacaggctt cttacatgat
    11101 gttgttgaag atacagaaat cactttagaa gatctgagag aagaatttgg cgatgacgtt
    11161 gctatgttag tggacggcgt aaccaaatta gggaaaataa aatataaatc ccacgaagag
    11221 cagctagcag aaaaccaccg aaagatgctg ctagcaatgg ctcaagattt acgagttatc
    11281 atggtcaaat tagctgatag actacataac atgcgtacgt tgaagcacct gcgagaagat
    11341 aagcaaagaa ggatcgctca ggaaacttta gaaatctatg cgcctcttgc acatcgtcta
    11401 gggatcagcc ggatcaaatg ggaattagaa gatacagcac ttcgttatct aaatccaaaa
    11461 cagtattacc gtatcgtcca cttgatgcag acgaagagag aagaacgtga aaaatacgta
    11521 agtggtactg ttgaagatat tcgaatagcg acagaagagc tggggatttt tgcagaaatc
    11581 tatggacggc caaaacacat ttattcaatc tatcgtaaaa tgaaagatca gaaaaagcag
    11641 ttcaacgaaa tttatgactt gctagctatt cgagtgatcg tagattcaat caaggattgt
    11701 tatgctgtat taggagcaat ccacacaaaa tggaaaccaa tgcccggcag atttaaggat
    11761 tatattgcca tgccaaaagc taatatgtac caatctttgc atactaccgt gatcggtccg
    11821 gcaggaaatc cggtagaaat tcaaatccga acacaggaaa tgcatgaaat cgctgaattc
    11881 ggggttgctg cacactgggc ctataaagaa ggaaaaaatg aaaaagtaga accagatggt
    11941 atgacgaaac aattaagctg gttccatgag atactcgaac ttcaagacga aagctatgat
    12001 gcttctgaat ttatggaagg cgtaaaagga gatatcttta gtgataaagt ctacgtcttc
    12061 acaccaaaag gagacgttac tgagttacca aaaggatccg gaccattgga ctttgcatac
    12121 agtatccata cagatatcgg taacaaaacc actggtgcaa aagtaaatgg caaaatggtg
    12181 cagcttgatt acaaattgaa aaacggagat atcattgaga tcatgacttc tccaaattca
    12241 tttggcccaa gtcgcgactg gttgaaatta gttgctacta gcaaagcaag aaataagatc
    12301 aaacgtttct tcaaagccca agatcgagaa gaaaatgtga tcaaaggcca cgaatccgtg
    12361 gtcaaatgta ttacagatct aggatttacg cctaaagata ttttgacgaa gaacaaactg
    12421 caagaagcac tcgatcgttt taattatcaa acagaagatg atctctatgc agctgtaggg
    12481 tatggagaag ttagcccctt gacgatggcc aatcgtctga ctgaaaaaga acgtaaagaa
    12541 caaaaaatcg agcagcaaaa gcaagaagca gaagaaatca tgaatcagcc gaaaaaagaa
    12601 cctgacaaaa tgaaagtacg tcatgaaggt ggcgttgtca ttcaaggggt agaaaactta
    12661 ctaattcgta tcagtcgctg ctgtaatccg attcctggtg atgatatcgt tggttatatc
    12721 actaaaggca gagggatatc cattcatcgt cgagattgtc cgaatgttca gcctgacaaa
    12781 ccaaatgtag cagaacgttt gattgaagtc gaatgggaag atacatcgaa tacacgaaaa
    12841 gagtatgatg cagatttgga aatttacggc tataatcgtt caggcttatt gaatgatgta
    12901 cttcaaacag tcaatgcgct aacgaaaaat ctcaacagcg ttgaagcacg gacgaataaa
    12961 gataaaatgg cgacgatcca tttgacggtt ggtatccaga atttatccca cctaaagagt
    13021 atcgtggata aaatcaaagc agtacctgat gtctacagtg tacgccggac gaatggatag
    13081 gaggacagat atgagagcag ttgttcaacg ggtaagtaaa gctgctgtgt cagtcgatca
    13141 gcaagaaatt ggaaaaattg gctacggcct acttgtttta ttaggcattc atgaaaccga
    13201 tacgcaaaaa gatgtggact atctggtaaa aaaaattgca caaatgcgta tttttgaaga
    13261 tgaacaagga aaaatgaata ttagtgtaga agatgtggga ggacaaattt taagtatctc
    13321 tcaattcaca ctgtttgcgg atacgaaaaa aggaaatcga ccaagctttg tggaagccgc
    13381 acgaccagaa acagctattc ctttatacga atcattcaat gaaggaatca gaagtcgcgg
    13441 catcacagtc gctaccggac aattcggagc agatatgtcg gtttctctcg tcaatgatgg
    13501 acctgtaacg atcattattg attctaaaaa caaataaagt aatctggttt tcactagtaa
    13561 aaactatgaa acacgtcgct taagttagac tgataaactc taacttaagt ggcttttttg
    13621 tctgcaaaaa gcgcttcttt tgtcagcagt cggaaaaaaa gttaagctta aattatacaa
    13681 gttatttttt ataggaggtt gctgaagatg aaagaagtac cagtagtaga tatcaaaatc
    13741 acagatttaa aaaaattgta tcaaaaagga tacaacatgt tggaggagtt acgccatgaa
    13801 gcggacgctc ccgtcgtcaa agcaaagatt ttcaataaag aagctatcac gatctacgga
    13861 tcatcagcag caaaagtatt ttatgatcca aggaatttca aacgaaaagg tgctatgcca
    13921 aaattagtat tgaagacact ttttggtcaa ggtggagtac agactttgga tggagctgct
    13981 catcaccatc ggaaaaatat ttttatggat ctaatgacac cggaaagaat ggaagattat
    14041 catcgtatct tagataagaa tctgacacaa gcacttgaag cacagcatgg tcaatttgaa
    14101 ctatttgatt taagtaagat ggttttcttt acatctattt gcgagtgggc aggtatcaat
    14161 ttatcagcga tttctaaaga tgaggtcgaa aaattagcag aatatcaaat ctctatgatc
    14221 agcggaacgt ttacttctcc gattgatcac ataaaaggtg tcgaaaatcg caaaaaatca
    14281 gaaaaatggg cgcaaggctt gattgaagaa gctcgacaaa atcctgtggc tggaaaagaa
    14341 aatgtggcat tatatgcttt tgccaatgcc acagatttag atggtcagct tttacctctg
    14401 gaagtggcag ctgttgaatt attgaacatc atccgtccaa cggttgctct aactgtgtgg
    14461 gcggcattga tgggacatgc acttttcagt agaccagatt tgtatcagca attaaaaaat
    14521 gacttttcga cgttacaaga tccgtttatt caagaaatga gacgctatta tcctttcttc
    14581 ccaatgctac ctgcgatttc gctgaaagaa gtagaagtag atggttatcg tataccagaa
    14641 ggcagttggg ttattttaga tttatatgga acagaccatg atgaacgaac agttgaagcg
    14701 ccagattcct ttatgatcaa acggtatgta gggaaagcaa aagatatttc ctataaagaa
    14761 gaatatgaga tgattgcaca aggcggggga aattttcgac aaatgcatcg ctgtgcagga
    14821 gaatggatca ctcttcatag tctacgtgtg ttttccgatc agctagtaaa taagtttgag
    14881 ttctctgtac ctgaacaaga ttggacgatt ccattcaatc agtttccgac ttatccgaat
    14941 agccgagcgc tactttacaa gaattagatt agcataaact tatcgaaaaa aaggaagatc
    15001 aaacagtgct gatcttcctt ttttcatatt actataaatt gataaggtca attgatcaaa
    15061 atccacgaaa tgcttcatca atggtttgat aatcttcatt ggataaatgc aaatctaaag
    15121 cttggacatt gtcagatact tgctctggac gcttagctcc aggtataact acagacactc
    15181 gtggattctt catgtaccaa gctaaaacaa gttgcgcagg agtggcttgg tatcttttag
    15241 caatggggcg aagtacatcg acagcggtta aaatttctct gaaacgttct ccttggaaat
    15301 ctggatttct ttttcgagga tctccttcac caaattgttt ttcctcacca agttcatatt
    15361 ttcctgtcag caaaccggaa gcaagaggaa aatagggaac aaaactgatt ttgttttttt
    15421 caagataagg gaataattct ttttcagcct gccgatggat caaactgtat ttatcttcta
    15481 ctacatcgac atatccatct gcattcgctt ctttcaattg ttctaacgtg aagtttgaca
    15541 caccgactgc tcggattttt cctgcttctt ttaattcgtg caatgtagca actgattcat
    15601 tttttggtgt gctttcatcc ggaaagtgga tatgaaaaat gtcgatgtaa tcagtttgca
    15661 gacgttttag tgcatcttcc accgattgtt tgagaaactc aggagaatta tcaaatgttc
    15721 gccctttatt tggtacatga gcagcttttg tagcgatgac gacacgtgag cgatcatatt
    15781 ctttttcctt cagtacctcg ccaatcagtt cttcggaacg gccgtttccg taagcataag
    15841 cggtatcgat caaattgatc ccgctattca aagctgtacg aactacttgt tttcctgtct
    15901 catcttccaa accgtcaaaa agattatgtc ctccaacggc atttgctcct aatccaagct
    15961 gttctgcgta gacttgtgaa tgtccgattc ttatttctgc catagctgat tcctcctttt
    16021 tgtctagtct aaaacaaaat accaaaaagc tgaactaaaa cgtttttgtg aggggaaata
    16081 gtcagatatc tcttgacttt gagactggtt tttagtaggt tataagtaat atcgaatgtc
    16141 aatgaaagag cgagtaagtt tttgaaactt tacaagagag gatcatcgag gctgaaaatg
    16201 atctaaagtt tcattagcct aaagacactc tggagatgga cgagtgaaaa gccgtcccgc
    16261 gtacgagcgt tattcgtaag aggaaagaga aatctttcaa aattaggtgg taccgtgtat
    16321 gaatttcatt cacccttgtt catgatgaac aagggtgatt tttttattta catagctaag
    16381 aaagggagag aaaactatga gtttccaaag accaaaagga acaaatgata ttttgccagg
    16441 cgaatcagaa aaatggcagt ttgtcgaaga aacagcacgt ttattattca gagattatca
    16501 gtataatgaa atgcgtacac cgatttttga acactatgaa gtgatatccc gaagtgtagg
    16561 ggatacaacg gatatcgttt caaaagagat gtatgatttt tatgacaaag gggaacgcca
    16621 tgtgacgctt cgaccagaag gtacagcacc aatcgttcgt tcttttgttg agcataaatt
    16681 gttcggccca gagtatgcga aaccgttcaa aactttttat atgggaccaa tgtttcgcta
    16741 cgaacgccct caaaaaggac ggttacgtca attccatcaa atcggagtag aagcatttgg
    16801 atcagaaaac cctgcaactg atgtggagag catggtgatg gcacttgatt tcttcaaaca
    16861 gttggggatc acgcaaatcc ggctagtcat caattcatta ggtgataaag aaacgcgtca
    16921 agcctatcgt caagcattga tcgactatct tttacctttt gaatcagagc tgagtgaaga
    16981 ctctaaacgc cgccttcatg aaaatccatt acgcgtattg gacagtaaag acaaacgaga
    17041 tcaaaaattt gtggcagaag ctccatctat cttagattat ctaagcgagc ctgctcaaaa
    17101 acattttgat accgttgttt ccatgctgga tgcattggct gttccttatg aaatcgacag
    17161 taatatggtt cgcggactag attattacac acatacgatt tttgaaatca tgagtgacgc
    17221 accaaaaatg ggggcacaag caacgatttg cgcaggcgga cgttatgaca atctagtgga
    17281 agaactaggt ggtccttcaa cacctggctt cggatttgca atgggtatcg agcgattact
    17341 cttgactatg gaggcagagg aagtagttat ccctgcattt aatgagttgg atgcttacgt
    17401 ggtcgcactc ggagacgaaa caaatatcga agcattgaaa gtggtccaag cgattcgaaa
    17461 ctttggtttt tcagctgacc gcgacttcat gaaccgtaaa gcaaaagcgc aatttaagac
    17521 agcagataaa gcgaatgcca aattagtatt ggtgatcggt gaagatgagc ttgctagtca
    17581 aacgattaat gtcaaagcaa tggcaagccg aaaagaaaaa agttttccat tagaagagat
    17641 atatgaacat tttgatgaag tctatgatga aatgacttta ttcgagtagg aggaaaaaaa
    17701 taaatggcac aaagaacgat ctattgcgga ttagtttccg aacaacaatt agaacaaaca
    17761 gttacattaa aaggatgggt acaaaaaaga cgagaccttg ggggagtaat ctttatcgac
    17821 ctacgtgatc gtgaaggaat cgttcaaatc gtctttaatc ctgaaatcaa caaagaagca
    17881 tgggaaatcg cggataaatg tcgcagtgaa tacgtgatcg aagtgactgg ggaagttaga
    17941 agacgcggag aaaatgcagt aaatcctaaa atggccactg gtgaattcga agtgattgca
    18001 agtgaaatcg tgattttgaa tcaagcaaaa accccgccat ttcaaattga agatgatcaa
    18061 gcaatcagtg acgaaatacg tttgaaatat cgttatttgg atctgcgtcg tccaaaaatg
    18121 actaaaaaca tcaaattaag aaatgcgaca acaaaagcga ttcgtcatta tttagatgat
    18181 aatgactttc tcgatatcga aacaccttac ctagggaaat ctacaccaga aggcgcaaga
    18241 gactacctag ttccttcacg tgtccatgct ggacatttct atgcgttgcc gcaatctcct
    18301 caattgttta aacaattgct gatgaatgct ggattcgacc gttattatca aatcgttcgt
    18361 tgtttccgcg atgaagattt acgtggagac cgtcagcctg aatttacgca agtagatatt
    18421 gaaacaacgt tcttaacagc agaagaaatc caaacttaca cagagggatt gattgcgaag
    18481 gtaatgaaag aagttcgcgg tatcgaagtc acattgccat ttcctcgtat gacctatgat
    18541 gaagcaatgg ctcgctacgg cagcgacaaa ccggatacac gttttgatat ggaattgatc
    18601 gatctaagcg atacagtcaa agaagtagaa ttcaaagtct tccaaatggc tcttgaaaac
    18661 ggcggtgtcg tgaaagcatt gaatgctaaa ggcgctgcag atcgctactc acgaaaagac
    18721 atggatcagt taggtcaata tgtaggacaa ttcggagcta aggggcttgc atggctgaaa
    18781 gtagaagaag acggattgaa aggtccaatt gctaaattca tgggagaagc aacagaagca
    18841 atcattaaag caacagatgc aaaaccaggt gacttgctga tgtttggcgc agataaaagt
    18901 gaaatcgtcg ctgctgcatt aggtgctatc cggacacgtt taggaaaaga attaggacta
    18961 attgatgaat cgaaattcaa tttcttatgg gtaactgatt ggccacaatt tgaattttca
    19021 gaagaagaag gtcgatatgt ctctgctcac catccattca cgatgccaaa agaagaagat
    19081 atcccgttat tggctgaaga ccctgcaaaa gtctatgcag aagcttatga tatcgtcttg
    19141 aacggttatg aactaggcgg aggttctatc cgtattcata ctcgtgagct tcaagaaaaa
    19201 gtactagaaa ctctaggctt tacaaaagaa caaatggaag aacaatttgg tttcttgtta
    19261 acagctttag attacggatt ccctccacat ggcggtattg cattaggatt ggaccgtttt
    19321 gcaatgttat tagccggaga agataatatt cgtgaagtca ttgcctttcc taaaaacgga
    19381 aaagcagcag atgtgatgag tgatgcacca agtatggttt cgcctttaca attgttcgaa
    19441 ttgaatattg atgtaacgaa tatagaagaa tagccgataa aaaggtaaaa aggatttgtc
    19501 gaatacgctt cttttgacaa ccttgattta attgaaaaac actttataat gaagccataa
    19561 catataaagc ggggcttttc catgaaaaaa acattcgaaa attgggctta tcatgattac
    19621 acgaccaagc tttcggtatc tatcgtttat gcgttgatcg catcagtcgc attgaatttc
    19681 ttttatcagc caggacatat ttattcaagt ggtattactg gtctagctca gattttgacg
    19741 actttgtcaa aatcactgat aggattcgaa gtaccggtat caacaacttt gtatttactg
    19801 aatatacctt tgtttgtatt ggcgtggtta aaaatcggga agaagtttac aattttcact
    19861 tttatcacag tcactttgac ctccctttgt atccatattt taccggaaac agtcctatct
    19921 accgatccaa tcatctgtgc gttgtttggg ggggctgtga tgggactagg tattgggcta
    19981 gcgttgagaa acggcctatc atcgggcggt ctagatatcg tgagtatcac gattcgtaaa
    20041 aaaacaggaa agtcgattgg ttctatttcg atctacttca atgcattgat cgtttttgtt
    20101 gccggaattc tatttggctg gcaatatatg ttttacagta cgttgtctat tttcgtcagt
    20161 ggaaaagtaa ctgatgtcgt ttatactaaa caaaagaaga tgcaggtaat gatcgtaacg
    20221 aaacatccag aaaaagtagt aaatgaaatc caaaactgta tgcgtagagg gatcacgatc
    20281 atcaatgaag cagaaggtgc ttataagcat gataagcaga cagtattgat gacgatcgtt
    20341 acacgatacg aactgcctct acttagagaa gcaatgaaag cttctgatcc acaagctttt
    20401 gtaagtattg cagaaaacgt ccagatactt ggtcgtttct atgaggatgg catttagatc
    20461 gagtttgaca aaaattcatg aaatactgct gtagacagac gttcgtttgt ctgcagtttt
    20521 tttctaccat agtagaatca gcaaagaaaa actgcttttt tatttgaaat tttcttaaag
    20581 ttgaaaaaaa gcatgtttat ttttttaaat ttatgggatg atagaaaaaa atagtagaga
    20641 aaggacacga ttctacttgc tattttcact taatacccgt atattacgaa tgaaaaaagt
    20701 ttatgaaaag ttggaatatt gaatggaaaa acaaaaaagg ttaaaaaaca gccgctacat
    20761 cactgggttt gatgggctcc gttcattagc tgtggtgggt gttatcttct atcaccttct
    20821 gccaactagt atgaaaggcg gctatttggg cgttccgatt tttttcgtcg tatctggtta
    20881 tttgatcacg gatctgctgc ggcaggaatg ggaacaaaat ggaaaaatca atatttggca
    20941 attttatgtc agaagaatga agagactcta tccggcactt gtttttatgc ttgtgacagc
    21001 ttcggcttat atcactttgt ttcagcgagg gctgttaaac aatcttcgag gagttgtggt
    21061 cagcagctta ctgtatgtca acaactggtg gcaaatcaat aacggtttat cttatttcga
    21121 tcgatttgct aatgagtcac cgtttacgca tatctggtct ttagcagtag aaggtcaaaa
    21181 ctatttggtt tggccgatct tgtttgtatt gttgatggta tttgttcgta aaagaaaatg
    21241 gatcttttat actgtgttag gtacatcctt cctctccgcc atcttgatga tgatcttgta
    21301 tacaccagga ggcgatccaa cccgtgttta ttacggtacc gacactcgtc tgttttctat
    21361 atggttggga agtgcgcttg cgtttgtttg gccaagtacg cgtttaaaga aaaacatccc
    21421 gaaacaagcg aaaaatattc tgaatatcgc tggaggaatc tcgcttttgg cactgattct
    21481 ttgcttcttc tttttagatg atcattacaa tttcatctat tacggaggaa tgctcctagt
    21541 gagtctgtta tgcgtagtgc tagttgcagt gacagcacac cccggagcaa gtttgaatcg
    21601 atggctgaca aatccagtat ttacttggat tggcaaacga agctatggca tttatctgta
    21661 tcagtttcca gttatgattt tttatgaagc aaaggtgact gatattgccg atcatgtttt
    21721 gttgcatact ttagtggaaa tcacattgat tctaatcatc agtgaacttt cttatcgata
    21781 tgtggagcgt ccgctagcac gttttgatta tggtcgtaca ttccaagtga tgaaaggatg
    21841 gttcagcaaa ccgatcatca gtaagagcaa accatgggtt gttccaggca cattacttgt
    21901 agcaattgca ctcgttggtt tcgttacggc accgaaaaac gctgtaacag ccgatcaaaa
    21961 acaactgcag gcaaaaatcg ctgaaagtaa aaaattagca gaagaaacca gaaacaattc
    22021 agaaaacacc actagttcgg ttgaccaagc gatccttgat aagtacgagt tgtccaaggc
    22081 acaagggaat aaggcacaaa aattagaact aacggcattt ggtgattcag ttatgttgga
    22141 tgctgcaacg gatctacaag aactttatcc aaaagtagtg gtagatggag atgtcggacg
    22201 acaattatat gccagtgtac cgtacattga aaaattaaag gaacaagatt tgttgaaaga
    22261 cactgttctg atcggactag gaacgaatgg ctcattctcg gaagctcagt ttgatagtgt
    22321 gatggatgca atcggtgatc gaaaagtata ctgggtcaat gtccgagtac cgacgaagcg
    22381 ctggcaaaat gacgtgaatg cgatgcttga acagatgacg aaaaaatacg acaatatgac
    22441 gctgattgat tggtacagct acagtaatga ccatagtgac tggttttatg atgatcaagt
    22501 ccatccaaat cctgaaggga tgacacatta cattcactta gtcagcgaag ccttgctagg
    22561 aggaagttca agtacgaaca gcagtgagac aaacgtgaca gaatgatagc tacaaaaaac
    22621 ttcaggcaaa aacatttgtt tttgcctgaa gtttttttat atttaccgtg tttttaacat
    22681 tcatatagat tcaggaagca aaacggttga gtgtgtctgt aaataacgtt ctttttgttt
    22741 tcgggtgagt tttttgaatg cagcttctgc ttttgttgct tcgcttcttg aagaaaatac
    22801 ttctgcatgg atcatcttaa caggtcttct tttttgaaga cgagtatatt tagcaccagt
    22861 accattgtta tgttcctcta atcgtctaga aagatcggtt gtatatccac cataaaatgt
    22921 ctgatcttta cagaataata cgtaaaaata atgttcattt gccatagagc atctcacgaa
    22981 tctctgcagt gtattctcca tccatattgt aagtgatcaa tggtggagcg actttgaatc
    23041 cgtctaattt gccttctttg attccttcaa ctaacaaaat atttgcctct tttccaggtt
    23101 ttgggtaaac aaattggatc cttttaggtg caattcgata gtcttccatg acctgaatga
    23161 tttctaagaa ccgatcagga cgatggacca tagtaaagcg tccatttgtt tttagtagct
    23221 tacttgaaat atcgatcact tcttctaacg tcgtatgaat ctcatgacgg gcgattgcca
    23281 ggtgtttatt agggttcgtt ttatttgttg gcagcccttt gaaataaggt ggattacaga
    23341 cgactaagtc acaagaatta tgtcgaacga cagataagct gtctttcaga tcgatggtgt
    23401 ggacagtcat ttgttcattt aaatggttaa gcgcgatact acgtcttgcc atgtctgcta
    23461 gacgaggttg aagttcgata gcgtcgatag ccgcctgggt tcgtttactt aaaaaaagtc
    23521 caactgcgcc atttcctgca caaaggtcaa caatcttccc ttttttaggt acacgggaaa
    23581 agtgagcaag cagcacagca tctaaagaaa aagaaaacac tttggaactt tggataatct
    23641 tgatatcttc tgcgtataat tggtcgattc gttcgtcttc gtgaagcatg gattccctcc
    23701 aaaatgaaaa aaatagtcgt ctgtattata ctatttttat taaaaagaac caaggtggct
    23761 tgtcaaacct ctatttttta tgcatactat gaccataaga agggaaagga agagactatg
    23821 ttttatcgat ttatgcgcgg ggtagttcga gtactattag ccttagttaa tggaaatgcc
    23881 cgctatgaga ataaagaagt tttaccgcaa gatgagaatt acatactagt tgctccccat
    23941 cgtacttggt gggacccatt atatctggcg gtagctgcag cacccaagga attcagcttt
    24001 atggcaaaag aagaactgtt caaaaatcca gttctgcgat ttatattaac tcgatccaat
    24061 gcttttccag ttaaaagaga aaatccagga ccaagcgtca ttaaaacacc tgtaaagatt
    24121 ttgaaatcga caaatttgag tttgatcatg tttcccagtg ggacaagaca tgctacagaa
    24181 cttaaaggcg gaatggcatt gatttccaga atgtctaaag cgaaaatcgt gccagctgta
    24241 tatcaaggtc cgttgacact tggggatctg ttcaagcgtc agagagtgac agtgcgtttc
    24301 ggcgaaccaa tcgacttatc agatatcaag aaaacggata aagaaggtat ggcggagatc
    24361 gaacgacgaa cacaagcagc ttttgacaaa ttggacaaag aagtgaaccc tgatttcaaa
    24421 tatgaagtca aaaaaaaata aaatgataag tttatgcgtg ggcggtcttt tgaatctact
    24481 gtcctgttca gttcagactt tttcaatgaa aacgagggag agacgtcaag tctgccctct
    24541 ttttgcgttc caatatgtaa aatagaagac aggtgtcatg taatggatca aagtgttata
    24601 ataattagaa ggacattttt tgaggtgatt gaatgcgttt tttacataca gccgattggc
    24661 atatcggaaa aaaactacaa ggttatgact tgattgaaga ccagcgtcat gtactatctt
    24721 ctatactaga tatcgcgaaa gaagaaaaag tagaggcaat cgtgattgcc ggagacttat
    24781 atgataggag tgtcccagct gttgatgccg tagaattata caatgaattg atggtcgatt
    24841 ggaatttaaa ggaaaagatc ccgatttttg ctatatcagg aaatcacgat agcagtacga
    24901 gactttcagc gggtacgcca tggttcagtt attcgaacta ttttttgcga acacagctag
    24961 cagaagcatt tgaaccagtc gagtttaaaa acgtacaatt tttctttctt ccttactttg
    25021 aaccaattgc agcgagatta tattttgaag atgacaatat ccgaacgatc aagcaagcgg
    25081 tagccaaggt gatagataaa atgcaggagc tatttgattc agaaaaaaaa caagtattga
    25141 ttagccattt ttttgtaatg ggaagtgtaa aatctgattc ggaaacgaaa atcgaagtag
    25201 gcgggttgga ttcgatccct ggtgaactac ttacaccgtt tgattatgta gcactaggac
    25261 atttgcataa tcaagctgct ttgagacagg ataatgctcg ctatagcggt tccccattga
    25321 aattctcttt atctgagatc aaccaaaaaa aaggcgtatg gatcgttgac ctgacaacag
    25381 gcctggagtt gaattttagg gagatcgaac ccctgcatga tattgaagaa ataacagcga
    25441 gtttcaaaga attgctggat cctgcctttt atcagtccgt caatcgggag aattacatct
    25501 atatctatct cactgataga acgatcatcc cgaatatgat gaaccagctt cggcaaatat
    25561 atcctcgtat tttggggatt gaacgggcaa acggtcgagc agaacaaaca aagcgcaaac
    25621 gaaaagtact agaaatgaaa gatcctcaaa aattagcaga agcatttttt agtgaaatga
    25681 cggaagagca gctaactgca aaccaagaaa aatggctcgg agaaacgttg caagaaatca
    25741 tggaaatgga atgatcaaga atgaaaccga aaaaattaca acttaaaaat tttggtcctt
    25801 ttatcaatga gaccgtagat ttttcacgtt tgacagaagc accgcttttt ttgatcagcg
    25861 ggaagacagg agctggcaaa acgacgattt ttgatgggat caccttcgca ctttttggtg
    25921 aaacttccgg aagacttcgc tctggaaaag agatgcgttc tttgtttgct acaccagacg
    25981 aagaaacaag cgtgactttt tcttttgaac atcagcagat gagctatgaa atcgaacgaa
    26041 aaccagaaca agttttagca aaacgaaaag gaaacggaac aaaaaaacag gcagccaaag
    26101 taacgttgac catttttgat gggaaaggaa aagaaataaa acaaatcaca aaacgtacag
    26161 aagcagatca gttgatcaga gaactgatga atctcgatgc gaaacagttt tctcaaatcg
    26221 ttttactacc ccaaggagaa ttccgtaatt ttttggtttc atcaagcagt gataaagaga
    26281 ctgttttacg tcatttattc ggtactcaat ttttccaaca gttcaatgag cgattaaaag
    26341 aaaaagcaaa acaacagcaa caaaaattgg atcatttaga gcaagagctg catcttctgc
    26401 aaaaaaggtt tgttttagtc aaagaagagg aaccagcagc agggtttgaa gccgttttag
    26461 cccaatggac aaaccatcag gcaatactcg aaaaacaaat cacggaagaa aaagaagctc
    26521 taacggaact tcaggaaaaa caaaagcaac tagaaaattc ctactatcgt tttgaaagct
    26581 tgaaaaaaag ttatgaagaa aaaacgatat taataaacaa gcaaaaggaa ttagctgctc
    26641 aaaaagacga aatagaggaa aagaaacgat ggattgggta ttatgaattt acgcaacgat
    26701 tagtcgaacc aatccagcgc ctacaagaaa taaaagttga aaaaatagaa ctgtcacaaa
    26761 aaagtagtga gcaaacaaag aaactagcga tgactacgca gaattatgaa gagtggcaaa
    26821 aaaatgaagc aaaccgcgta cgccgacaaa caaaaatcaa agaagctgct caaaaactgc
    26881 aaaacaaaca gatgatggtg ccaattgtcg aatccttacg tagtaaaaaa caagacgcag
    26941 aacaattaac aatttcattg gataaagaag aattgaatgc cgagaagctg tatcaaaatc
    27001 tcgctatcta tcaggaaaga actcgaattc ttcaagaaaa tttactggat caggaaaaac
    27061 tgcaagaaga tcgtcttgct tttggacagt taaaatatct agatgaaaag cagcaagaaa
    27121 tagaaaaaca aaaagcaaaa gacactgggt cgctcgcaca gataagagaa gaactcaaaa
    27181 aagcaactga agaaataaca aaaaatagtc agctattgaa agaacagaca gaacaagcag
    27241 aacagctgaa aagccaatgg gcaaagcagc aaattgctcg tcttcagctt tttttgcgtc
    27301 caggggagcc atgtcttgtt tgtggatcaa cggaacaccc catacaacaa ttaaaacacg
    27361 aacagccaaa taaggaagat atcttaaaag tagaacaaca actggcagaa gctgagtctg
    27421 ctgtagaaag aactcagcaa atcattgcca aaagtgaagc acatgaaaat aatcttcaac
    27481 aacaattatc agatttagaa caaaaaatca tggcaacgaa tatacaattg gctgaacaag
    27541 aagaacactt gcaacaggct ttgaaaaagg catttcctga gataacagaa caaaaaacaa
    27601 aaacaactct tgtagtaatc gaacaatctt tagatagacg aaaaagcaag atggccaatg
    27661 caagaataga gctggaagaa ataagacaac aagcagaaaa gctcgaacaa caattagctg
    27721 tctacaaaca gcagcagaca gaaaaaaata atcagctcca gcaactgcaa ggagaaatca
    27781 aaagtttaga acaacaattg caagatacgg atagtaactg tttagaagag gagatacgac
    27841 gacttgaaaa acagttagaa gaagactcga atcaactagc tgttgagcag aaagcaggag
    27901 tgcaattgtt gcaagatctt catacgcttc ggacacagct tgatcttcaa catgaacaaa
    27961 tagaaaaaat agatgaaaaa atagaaaagg aacaagaaag aatagctaaa aagttagaaa
    28021 gccagtcgta tttttcaaca attgaagaaa tctgtgcatt tgcacaacag caagcacaat
    28081 ataaagaaaa ccaacgtaca gttacttctt atcatgaaga gcaattagtg acagctgacc
    28141 ggctgcgtca attagaaaat atccagctaa ctgagacatt cccagataca gaagagatca
    28201 gccaacagtt agaagaaata agacgtatga tcgaaataaa gcaagaaaag atatacggtt
    28261 tacaagaaca aaaaattagt aatcaaaaaa tctacgatga atgtctttcg atttatcaca
    28321 caagccagac gaaattagat gagcttacgc aattacagca gctttcgcaa acattcaacg
    28381 gagaaaatcc taagaagact agtttggaac ggtatgtgct tcaggtttat cttcaagaag
    28441 tgttacaagt agcaaatgat catctccaac gtttaacgaa aaatcgttat caatttgagc
    28501 tagcggatac cattggcagt tatcgaggga aaaccggatt agaaatcaat atctatgatg
    28561 atcaggcagg tatgtctcga agtgcgcata ccctgtctgg cggtgagagt tttattgcag
    28621 cactttcttt agcattagct cttgccgatg tgatccagac acaagctggt ggtgtagcaa
    28681 tcgatgcatt gtttattgat gaaggatttg ggtcattaga tgaagaagct ttggaaatgg
    28741 caatggaagc tttagaaacg atcgaaagtg aaggaagaat gatcggcatc attagtcatg
    28801 taagagaatt gaaagaacga gtattacaac aaataagaat tgatacagaa ggtagcggac
    28861 aaagcaaaat acgctatcaa cttggataaa aagggagatt ggtaactatg aaatggagta
    28921 ttccagaaaa agtcattgca cgtggacgtc aatacttgca agaaggccga gtgttatcgg
    28981 ttgtccctga ccaagaagaa aacgtgtggc atgcagaagt cttgggcagt gaattgtata
    29041 tggttgattt ggatggtaca gcaaaagaga tagattactg ccaatgtccc tattgggagg
    29101 aacataaata ttgtaagcat acagttgctg tggaacttta tttacggtcg aaaaatctat
    29161 ctcgtatcat gaaaaaagac cagccaatgc tagaaccggt aaaagcaaca agtgagggag
    29221 aaatcattgc aaaaggtttt tctcgtctaa aaacacaaaa caatcctcaa gcggtgcaac
    29281 ctttgattat tgaataccaa gtagaaacga ttgagactaa tcagtatcac ccagagcttt
    29341 ctattttggc actttacttg cgtatcggtt cattggcatc accgaaaaaa acatatatcg
    29401 taaaaaacat ctatgatttt ttacaagctt acacagaaaa agaaacttac actgttaata
    29461 aacagtacca gtttcgtcta gataaacggg cgtttcaaaa agaagatcaa aaaatactca
    29521 cgcatcttgc aggaagtgct cagacgcatc aattattagg tacgaacggc ttacaagtca
    29581 aaggaaaact agataaaaga tatctgcttc tcccagtaga acaagcccgc ttcctgttag
    29641 agcagatgaa tcagactaca cgtttgcatc tgcagataaa tgaacaaaac gatcaaggaa
    29701 tacaattttc cgatcaaaac aaacctttat cattcaaagt ggaaaaattt ggagaagatt
    29761 atttgctgca agcattgaat gattttgatt ttttctttat gcattatcag tggggagtta
    29821 tccaagggac attttattct ctgacgaaat atcaacaaac ggtttatcaa acttggaaac
    29881 aattgattcg ccgattggag aaaccagaag tagtgttcaa gaaaaaagaa ttaccgtctt
    29941 tgtttaaaga gattatccct ctgctgtcag agatcggcga cgtagctatt gcccctgcag
    30001 tagaagagga agtagcagat atccccgtcg aatttatttt ctttttccga aaagcaaaag
    30061 gaaaaatcca agctcgagtc gatttcgtct atggcgaagt catctattcg actgacccga
    30121 agcatgaggt ctcgacagaa caggcttttc aggtgttacg tgacacagta gaagagaaac
    30181 gagtacttga tctattcaga atgtatcgct accagaaaaa tgaaactggt tatgaacgtc
    30241 cattgccaag cggggaagaa ctatatgcct ttttccgcac agaactatct gtcttccgtc
    30301 agtttggtga agtcagactt ggaagaaaat tgcgagagtt gtatttagat gcgcataaac
    30361 atcaaccgaa aatcgaagta accgagaccg gttcatggct cgatattcga ttcgatgtga
    30421 cagggataga ggagcaagaa atcgatcatg tcctgcaaag cttgctgaga aatgatgctt
    30481 tttatacatt agaaaatggt caggttcttt cttttgattc agaagaattt caacaaacaa
    30541 gccaagtgtt gcaacaatta cgggaatcga tacgtacaga agaaggaacg atccatgtac
    30601 ctaaaaatca aggattgatc attcaaaatc aattggaaaa aagcaatgct acttttagtg
    30661 aatcgttcca aacgatggta caagatctca ttcatccaga aagataccag gcacagcttc
    30721 ccaaaggatt gaatgcaacg atgagagatt atcaaaagca aggttttcga tggttgaaga
    30781 tgttaggtca ttatcagttt ggcggtattt tagccgatga aatgggactt gggaaaacac
    30841 tacagacaat tgccttttta ctttcggaaa aagaagaacg gaaaagcttc tctgctttga
    30901 tcgtagcgcc tgcgagctta atttacaact ggcaggcaga ggtgagaaaa ttcgcccctt
    30961 ctttaagtat ccaagtaatc aatgggaata aaaaagaacg tgaagaatta ctggctaaag
    31021 acacagatat ccgagtgact tcttatgcca gcttaagaca ggatctagct aattatcagt
    31081 cgcaaaaaat tgattatttg attcttgatg aggcacaaat ggtcaaaaat agcagtacga
    31141 aaactgcaca agcattgaga gaattagctg tacctcagcg gtttgcactg agtgggacac
    31201 caatcgagaa taatttagaa gaattgtggt cattgtttgc aacgattatg ccaggatttt
    31261 tcccgactaa aacaaaattc agggaacttg ccgcagaaga aattgcgcaa atgattcgac
    31321 cgtttatttt acggcgagat aaacaaactg tgttgaaaga cttgccggaa aaaacagaga
    31381 tgaatctata ttctgcactt acagaagaac aaaaaactgt ttatttagct tacttgcgtc
    31441 aaatgcgtga agaaatcaca tcaatggatt ctgaagcatt caagaaaaac cggatcggga
    31501 tacttgctgg tctgacaaga ctgcgacaaa tttgctgcga tcctcgtctg tttattgaag
    31561 attaccaagg cggttccggc aagctcgaac aagtaaaaga tctactggta gctgccaaag
    31621 agaacaaacg aagagttttg ctgttttctc aatttacagg gatgctgact atcttgcaag
    31681 aagagttagc cgagctaggg atctcgacat tttatttaag aggcagtacg aaaccacaag
    31741 atcggttaag tatggtagac gcatttaatg ctggcgaaaa agatgttttt ctgatttcct
    31801 taaaggctgg gggaactgga ttgaatctca cgggagcaga tacggtcatc ttgtatgacc
    31861 tttggtggaa tcctgccatt gaagaacaag ctgctggacg tgcacatcgg atcggacaaa
    31921 aaaatgtggt agaagtgtgg cgtatgatct ctgaaggaac gattgaggaa cgcatggatt
    31981 cactccagca agaaaaacgc gaactgttcc aaaaagtgat ccaaggtaat gaagaacagt
    32041 tgacgaaact gactgaagaa gacatccgca tgattttaag tgtaggagaa atcgaggagt
    32101 aagagactcg aaaaagcgtt cagtaatggg taagaaagcc tttctaaaga agcgattgtt
    32161 tatttgattt aaaaaatagc aaggaggctg ggattccatt ttatcccagc ttccttgcta
    32221 tcattttttt atttctattt ttaaaggtta tacaaagatt gtcttcattg tctctaacag
    32281 ctgatcaaaa gaagaaatct cgatatcgca aattggagtc tgttcaattt tgcgttctcg
    32341 atggttgaac caaagcgctt gccagttagc actttttgca cctagaacat cattgtcgta
    32401 attatcgcca acgtataagg tctcttctgg aagcatatga aattctttct cagctagctg
    32461 aaaaatctct ttttctggtt tttggaaacc tgttgcttga gaaatgagca tattgcggga
    32521 aggaatccaa ttgttcaatt gaagttgctt cagttttttt gtctgatggt cagtcggccc
    32581 atttgtgata attcctaatg ggacattctt ttccttcaag aaatcgaggg tttttttgac
    32641 ctcctcatgc aagcagatat tatcaagttc ttcctcgtaa attttttgaa ataagaggcc
    32701 atcttcttcc gtaatatgag ggtagtctaa atcttttagt gattggctga tccgatgtgc
    32761 tcgcatatat tctagtgtcc aatctcctgc cattactttt gggaagtttt catcactatg
    32821 atggcggaac cggatatata gtggatgcat atctgcatct gaaacaagcg gaacgactct
    32881 tttgaccgcg tttcgaaatg gctgctgctg atcatacatc gtatcatcaa catcaaacac
    32941 aactgattta atcaaaattt tacacgcctt tcggttttca ttgatttttt cacaaatcct
    33001 tgactattgt aacagaaaaa attgtctaaa aaagctgata aataaaggtt tttcttacta
    33061 tatttctatt tccttgacag aataaggttt ttatatttca gtttttttca ctaaaaatcg
    33121 tgaaacacgt caataagttt gatgaacaaa ttaatcatta tggtagaagt cgattgattt
    33181 attttaatgc aatctctgtg gaattttcga aag
//
LOCUS       DAZKCZ010000022        32573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000022 DAZKCZ010000000
VERSION     DAZKCZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.022"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..626)
                     /locus_tag="ACG8KN_000888"
     CDS             complement(<1..626)
                     /locus_tag="ACG8KN_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFP9187967.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="ACG8KN_000889"
     CDS             899..3370
                     /locus_tag="ACG8KN_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9187968.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="ACG8KN_000890"
     CDS             complement(3473..5209)
                     /locus_tag="ACG8KN_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9187969.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="ACG8KN_000891"
     CDS             complement(5206..5910)
                     /locus_tag="ACG8KN_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9187970.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="ACG8KN_000892"
     CDS             6041..6544
                     /locus_tag="ACG8KN_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFP9187971.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="ACG8KN_000893"
     CDS             6504..6842
                     /locus_tag="ACG8KN_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFP9187972.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="ACG8KN_000894"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="ACG8KN_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9187973.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="ACG8KN_000895"
     CDS             8393..8971
                     /locus_tag="ACG8KN_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9187974.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="ACG8KN_000896"
     CDS             8975..9496
                     /locus_tag="ACG8KN_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFP9187975.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="ACG8KN_000897"
     CDS             complement(9575..10129)
                     /locus_tag="ACG8KN_000897"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9187976.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="ACG8KN_000898"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="ACG8KN_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9187977.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="ACG8KN_000899"
     CDS             10669..10920
                     /locus_tag="ACG8KN_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFP9187978.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="ACG8KN_000900"
     CDS             11045..11836
                     /locus_tag="ACG8KN_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187979.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="ACG8KN_000901"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="ACG8KN_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9187980.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="ACG8KN_000902"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="ACG8KN_000902"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9187981.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="ACG8KN_000903"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="ACG8KN_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9187982.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13797..13982)
                     /locus_tag="ACG8KN_000904"
     CDS             complement(13797..13982)
                     /locus_tag="ACG8KN_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187983.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            14019..14312
                     /locus_tag="ACG8KN_000905"
     CDS             14019..14312
                     /locus_tag="ACG8KN_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFP9187984.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(14400..14546)
                     /locus_tag="ACG8KN_000906"
                     /pseudo
     CDS             complement(14400..14546)
                     /locus_tag="ACG8KN_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            15155..17482
                     /locus_tag="ACG8KN_000907"
     CDS             15155..17482
                     /locus_tag="ACG8KN_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFP9187985.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            17479..18393
                     /locus_tag="ACG8KN_000908"
                     /pseudo
     CDS             17479..18393
                     /locus_tag="ACG8KN_000908"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            18466..18981
                     /locus_tag="ACG8KN_000909"
     CDS             18466..18981
                     /locus_tag="ACG8KN_000909"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFP9187986.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            19109..20407
                     /locus_tag="ACG8KN_000910"
     CDS             19109..20407
                     /locus_tag="ACG8KN_000910"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9187987.1"
                     /translation="MHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYP
                     SKDTAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLS
                     LPFYNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQ
                     FVTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQ
                     KETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHF
                     IKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKL
                     ISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQ
                     PKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            20613..21785
                     /locus_tag="ACG8KN_000911"
     CDS             20613..21785
                     /locus_tag="ACG8KN_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9187988.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(21947..22621)
                     /locus_tag="ACG8KN_000912"
     CDS             complement(21947..22621)
                     /locus_tag="ACG8KN_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9187989.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
     gene            22992..23414
                     /locus_tag="ACG8KN_000913"
     CDS             22992..23414
                     /locus_tag="ACG8KN_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9187990.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(23580..24773)
                     /locus_tag="ACG8KN_000914"
     CDS             complement(23580..24773)
                     /locus_tag="ACG8KN_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9187991.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            24997..25374
                     /locus_tag="ACG8KN_000915"
     CDS             24997..25374
                     /locus_tag="ACG8KN_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9187992.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            25362..25700
                     /locus_tag="ACG8KN_000916"
     CDS             25362..25700
                     /locus_tag="ACG8KN_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9187993.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            25834..26277
                     /locus_tag="ACG8KN_000917"
     CDS             25834..26277
                     /locus_tag="ACG8KN_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFP9187994.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            26539..27354
                     /locus_tag="ACG8KN_000918"
     CDS             26539..27354
                     /locus_tag="ACG8KN_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9187995.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            27364..28026
                     /locus_tag="ACG8KN_000919"
     CDS             27364..28026
                     /locus_tag="ACG8KN_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9187996.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            28231..29895
                     /gene="alsS"
                     /locus_tag="ACG8KN_000920"
     CDS             28231..29895
                     /gene="alsS"
                     /locus_tag="ACG8KN_000920"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFP9187997.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            29908..30618
                     /gene="budA"
                     /locus_tag="ACG8KN_000921"
     CDS             29908..30618
                     /gene="budA"
                     /locus_tag="ACG8KN_000921"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFP9187998.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            30748..31245
                     /locus_tag="ACG8KN_000922"
     CDS             30748..31245
                     /locus_tag="ACG8KN_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFP9187999.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            31430..31690
                     /locus_tag="ACG8KN_000923"
     CDS             31430..31690
                     /locus_tag="ACG8KN_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188000.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(32052..32291)
                     /locus_tag="ACG8KN_000924"
     CDS             complement(32052..32291)
                     /locus_tag="ACG8KN_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188001.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT        10964 a         5762 c         6524 g         9323 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatc agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaacgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt
    13861 ggtaggggag aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc
    13921 actatgataa tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac
    13981 ataaatggag tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc
    14041 aagaaaagat gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa
    14101 gttcaacaaa aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag
    14161 tgacttatac agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt
    14221 atgtcgaaca aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc
    14281 gggcatatca gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa
    14341 gttcaagtat gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc
    14401 tataatacaa agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag
    14461 cgattcgcag ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa
    14521 tcaattggat gcgttacatg aataaagata attctctttt atttgtacga ataaatacca
    14581 aaacgtacct cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc
    14641 gttcttgaaa caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc
    14701 tgagcgttag aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga
    14761 atacaagatc agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg
    14821 acacatttct cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat
    14881 aatcccgtcc taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa
    14941 ttaatcaagt tttatctttt tttttcaata atcgtaaaaa aagagtattt tttatttaaa
    15001 aataaaatct ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaaa
    15061 agccccaaac tttctataca tcaataaaat aataaaaatt aaatttagaa taacgtgtaa
    15121 aggattattt gatgattttt tattgagagg agggatgaaa atgattaaga aaactcaaaa
    15181 taaaatccaa cgtatatgtc aaaaacattt tgtttctaga ctcaaagctg tatctaaaag
    15241 aaaatactta catcccatta tgatttcgat catcttgatt tttactttct tagggagctt
    15301 atcaagtatt tatgtaaacg tttatacaga aacgtcaaat tttgctaacc aaacgacaga
    15361 ggagacaaat agtccaccct attctgaaat acagtccata gaaagcacca atcagtctag
    15421 caacaacgag acaacctcaa ccacatcctc gactcaactg attgatccga cagaaaaaga
    15481 aatcacagca gcaaaagcag caggggagaa agcttattca gaaacaggta gaagccagac
    15541 atttagtact gttgcggcca ctgcgaattt caacggacat accataccag atggatatac
    15601 agtgatatat gccaaagatg gagtcattgt tgcggttaac ggatcgatga gtgcggataa
    15661 aactgcgtta caaggcgtaa ccaaagcaat cgcttttatt gataaccgtc cagatatcca
    15721 taatctgttt attggctcca cagtaaatgg tactactatc acattcccaa aaagtatcta
    15781 tatgggggtg gctaccgctg gaaatacgat cgattcagtt acacccaatg cggataaaag
    15841 ttattctttt acaccctcaa catggggagg gcttcctgcc ggaggaggta tgagtacttt
    15901 ttcaatcggt atacagttag caaatgatat tccattcacg gggtactcga tcaaaaatgg
    15961 gcagatcgtt gatggtaagg gaaatgtttc aaattttcca acctatcaag cagcaacggt
    16021 tcaggtcaat gcgaatcgga caggaacttt ttcttataca gtcaatggtg gagcagcaaa
    16081 aatcggaacg tacgggacta cattatctgg aatccatgtg ggtgacaaag tggttatcac
    16141 tccaacagca gtaacgggct attcttttgc acaaacaaac ggttctttca cagccctaga
    16201 aagtaacgtc gatacggtga cctatacagg caaacaaaca ccaatcacta ttaagtatca
    16261 agactatttt ggacagatga ttgctccttc taaaataatg aatcttactt acggaagtgc
    16321 tgcacaagac ctgacgacta atacgcctac tatcaacgga tataccttta cagctgtttc
    16381 agcgacagaa acaaagaacc aatcagcaat gagtgtttta gctagtttag atacaaatgg
    16441 aaatgtaatc gtaaaggacg caaatggaaa acagatttct gaagtcatcc tatattataa
    16501 gacaaatgca acagtatcaa tcaatgcaaa tggcagtaag tactatgatg gtctttctgt
    16561 tgtaccgatc gttaaatatg cctttaatga taaaacagaa tcaacatccc ttttaaacaa
    16621 tgatctcagt attgatcaaa tcacatggaa tccaactgat ttcaaagcga tgaatgaaaa
    16681 gggagttgaa gtaactgcgc ctactgaaat tggaacctat acgtcttggc aattgacaaa
    16741 aagcggacta gctaaactag ctgccagaac aaactattta tttactatcg tgcagacatc
    16801 tgatgtctat acgattaagc aaatcagcgg tgcggtagag ctcagtggca gtaagacata
    16861 tgatggaaaa gcaggtgtcc cgtcaatcca tgtcaagcta gcagagggtg tcactagttc
    16921 gcttactcca gtacaagtgg cacttagcag tatggattac acagcagatg cacaatcagc
    16981 aaaaaatatg gttaatgctg gcagctatac aattaaacta acgaatagcg gaatagacaa
    17041 agtcaaagca gcaaatccca agttgtcatt tactgatata gccaacacat caggaactta
    17101 taccatctat aaagctaacg cagtgattac tgtcgatgac gctgccttta attatgatgc
    17161 ccaaagtcac tcgatttcgg ttggaaatct ccacgttacc ggtgttgtct taggcgaatc
    17221 attggattat accctaacag ttaacagccg gacagatatt ggatcacaaa tagtaacgat
    17281 tagtttaggc gaaggaagtg taaacaataa ttatacgatt atcgcaacag ataccgctaa
    17341 gttgacgatc aatccgactc tcttgcttcc ttcgacaggt gggataggtc tactgccctt
    17401 tgtcttttta ggactgatct ttattttttc tggcttcttt tattttatcc atcgtaagaa
    17461 aaaagcaggt gaacagcgat gaaaaaaaca aaaaatgtac gtgtggatat atttgctgtt
    17521 ctctttttta tcctactggt cttttttata ggttctccga ctgtgtcagc tagagaatca
    17581 cctattacat ttactttgca taatcgaatg ttcgaaagcg ataagatgcc aagtatcagt
    17641 caaaacgacg gaataaatat cccggataac tttcaaggag taaaagggtt gaacggcgtt
    17701 actttcgcta tatatgatgt ctcggatgaa ttttacaagc tgcggtcaga aggaagtagt
    17761 gtggaagacg cacaaagaaa actagcacaa aaaagtgata gtgaaaaaat actggcagaa
    17821 aatgtgacaa aaacagtaga cgaggaagag ggcattgctt cttttagtgc atcggacaaa
    17881 gatgagcaag gtcgtgacgc tgtttaccga tttgcggaaa taaagacttc agattaagta
    17941 aaagaaaaat cagcaccatt tgtcgttgtg cttcctgtga cagattcatc taatcataaa
    18001 ttgacgacca tccacttgta tccaaagagc gaacaaaaaa tacctgctgc actcacatta
    18061 acaaaaacag tagagaataa gcagacagat tttgcagatg gggataaagt tccttatctc
    18121 atcaccgtaa caattcctga gaacatcaac gagataggaa cctatacgat caaagataca
    18181 gctgatcctc agttatggct agaaccagaa acgatcgatt ttttgattgg tggagacaag
    18241 attcatacat ttcacacaca aaaaacaaaa catggttttg tcttatcaga ttctggaaaa
    18301 gatcttagtt cgtttgctgg gaaaaaatta acgatccgtt accaaatgac attgaaagaa
    18361 aacagtgaaa aaacgacatt tgataacgat gtacgtttga cgactgacaa tcaaacagta
    18421 gaaacacatc ttgccatcca aacaggaggc aaacaatttg tcaaagtgga tcggcaaacg
    18481 aaacaaaaac tagcagatgc tcaatttcta gtcaaaaaaa atgaccaata cttagcgcaa
    18541 gaaaacggga taaatcattg ggtagccaaa gatgattcaa gagcaacgat ttttacttcc
    18601 agtaaagatg gaacattttc tgtccatgga ttgtcttttg gaagttacga actagtggag
    18661 attaaaccgc ctaaaggata tatattgagt caatcaacca ttctctttga agttgaaaaa
    18721 ggcagttatg atccgcttca tcctatacca ttggaaatca tcaacgaacc aaaagcaaat
    18781 ccgacgaata atggaaaaac gccagttcag atgcagacaa atggtccatc aaatgaaaaa
    18841 aatggaggaa gctttccaaa aacaagcgaa gagatgcttg gaggtttctc aattttaggg
    18901 ttgttgctag tcctttcaac agggacagct tggttttata aaaaacaaca aaaaggaaac
    18961 aataggaggg aaataaaatg aaaaacatca aaacaaaaat atggacgttt ttaggaactg
    19021 ctatcatgct gcttcctttc gttctcggcc taggaactgc agaggtttct aggccgtatc
    19081 acctacacca gaaaatgtta cggttaattt gcataaattg aaattcactt cagcaccaga
    19141 aaatcaaata aacaacggaa cggaattgac atttccgaat agcgaaccgt tgaacggcgt
    19201 tgaattcaac gtatatgaca tcactgctac gtactatcca agtaaagaca cagcagttcc
    19261 agcagacgct acacccttcg caagtgtaac gacttctggt gaaggattag ctaacctgac
    19321 tcttccaggt aaatctgatg ggaaagatgc tgtatacgtc tttgtagaaa caccaaagcc
    19381 cggtgtcgaa acgtcgccaa atatcgtgtt aagcttaccc ttttataacc cgaattcatc
    19441 agaccctgac aaacctttag acacgattca cctatatgct aaaaatgtca tcaaagaaag
    19501 caatatcaaa atccagaaag ctgggaactt ttcaggggta gataagttag caggggcgca
    19561 attccagatc aaaaattcag agaatcaatt cgtgaccggt tttgatgaaa acactggtgt
    19621 cgctctctat gatcctgctg agtcagcgtt gacattcaca actgatactg aaggatccat
    19681 cagtgtcaat ggattaatag acggcaatta tactttagtt gaaacaaaag caccagatgg
    19741 ttatcaatta accaatccac aaaaagaaac tacttttata gtaaaaaacg gaaaattgca
    19801 acaaacaacc ggtatcatcc atggcgcaga tgaagccaat cttgggaatc caaacggtgt
    19861 agtcaatcgg gaaaatacaa ctacggataa cacgattact gtagaaaatg tccaaaacta
    19921 cggtgatttc cacttcatca aacaagatgt gaacacgcaa gaaaaattag ccgatgcaga
    19981 attttctatt ctaagagcaa aagatccaac tgttaaatta atgaaaaaaa tagcaaatct
    20041 tgcctcagga gaatacccat atgcttggtc agatgaagtc acaggttctg actgggaaga
    20101 agtgaaattg atttctgatt cagaagggaa attttcaatc gaagggctga aacaaggggc
    20161 atactgcctt caagaaacaa aagcaccaga aggctacgca ttacctaaga atgaagctgc
    20221 gtacactgaa tttacagtag atgatgatcc aacaacttct gatgaaacaa cgatcgataa
    20281 ccaacctaaa ggaatcttgc cttcaactgg tggtatgggg atcatcgtat tcatcttggt
    20341 aggtactgct ttagttggag gagcagtgat ctacttcaaa aaacgccatc aagaaacaga
    20401 agcttaaact attggaaaca aaaatcatac gataaataaa cgatagtgat tcaaatgggg
    20461 agaaaggttg tgacaagtgg tcggtctctt ggtattcgct caacaaacgg caaaatagga
    20521 ggaactattt tgccgtttgt tgagcgaata ctcaggtcaa ggcgctgatt tcacaacctc
    20581 ttcatataca tattagaaaa taggtggaca tgatgaataa aagcagaata agaatactgg
    20641 acatcttcat ggcaatcatt ttagtcgttg ggatcgggat cttttcttat ccttttgtag
    20701 aagattcact gaatgatttt cttgcccagc aaatgatcat ccattatcaa aagcaagcat
    20761 cgaataagaa ttcagaggaa ataaaaaaac agcaggaaaa aatgacaaag aaaaaccaac
    20821 agttggcaga gcaaaacgta tcaccaggga taggcagttt caaccaagca gttgatgcaa
    20881 aagcattaaa agatttacct agtaatgcct tttttatgga acatatgctt ggagtcatcg
    20941 aaatcccaaa aatcaatgtc agcttaccta tattcgatca gacaacggaa atatttttgc
    21001 aaaaaggaac gtcattattg gaagaaagtt cttatccgac tggcgggaaa aacacccatg
    21061 ccgttctttc tgggcatcgt ggactcccag aagcaaaatt atttacagat ttgcctaagt
    21121 tgaaaaaagg agaccagttt ttcatccaga tcaatggaaa gacattggcg tatcaagtag
    21181 aaaaaatcca agtggtctta cctgatgaag tagattcttt gggtatccaa aaaggcagag
    21241 atctcgtgac tttgcttacc tgtactcctt atatggtcaa tacacatcga ctgcttgtga
    21301 cggggcatcg tattccttat caagcaaaag aggcaaaaaa agcgatccaa ggaattgatc
    21361 aatggaaaaa atggaaattt tttatttggt ttatcggtat actacttgga tcaatcggct
    21421 tagtttggct actcatcgct tatttagatt ctttggccat cgctaagcga aactatcctt
    21481 tatcttttta tgtaaagaac acaaacggac gaccaatcga aggaatggtc ttttctgtaa
    21541 aaacgctcaa cggcaaacac tatattacta gagaaaaagt tccatttgtg aaagcttctg
    21601 atgaatacgg actagtgaga ttttctgatt taaaaggcag gaactatcgc ttgcaacatg
    21661 aggagctcct gcttaaaatc catgtaaaac ataaacattc gaaacagttt tcaatgaaat
    21721 tgaaaaaagg aagatataaa ctgcgaaaag aaaaagaggt gtactatttg attgagaaag
    21781 aatgaatgaa gatgtccaaa aaagcagagg gaacagacta aatgaaaaaa gagagaagct
    21841 gacgaaatgg acgtgtaaac tgctattttt ggtttactat ctggaacgcc agcttctatt
    21901 tttttatgtt tcagaagaat tttattatta tagtataact tttgaattag tttccataac
    21961 atttggagca aggggttaaa ccacgagctt gagcttcgct taaagtggca ggggtatacg
    22021 taccatttcc acatttatgt gtatggtatt ttgaaccggt cggtgtgaca tagacagttt
    22081 gttcattatt ttgctcagga gctgcggctt gtgcctgctg agaggcctgt tcagctgctg
    22141 cttgctgtcg cgcctgttct gcttgttgct gtgcgattgc ttcttgttgc gcttgttctg
    22201 cttgttgtcg tgcaatttct tcttgctttt tccgttcttc ttctgcttct tttgctttga
    22261 ttgtggaatc aattgcagca agacggcttg tcaactcttg tttgttttca ggtagcgctt
    22321 ggatagctgc tgaagcagtt gcatagtttt cttttgtagg atttgcttca gcttgtgaca
    22381 aagcagattc cgcttctgtc actttttttt ctagtgcagc tttttccgct ttttgtttct
    22441 cttcttcttt tttcttttca atttgttctt gttctttcga tgaatctgcc gtactttcgg
    22501 agatcgtggt actgctgctg gaagcctgag ctgaacttgt gctttgagta gtagacgtag
    22561 tagcaccaca cccactcaac aaacttcctg cgaccaaaaa caatgcaact aattttttca
    22621 ctaaatttcc tccttaagta aaaaacaacc gttttcaata aatgtccttt tcaattgttt
    22681 acttacttgt tataataatt gtgttatgaa tagtgattga ggaaacttaa tctaatcccg
    22741 ttttgataca cgggattttt tgctatagtt ctgttattaa gtatgcaata ataaaatatg
    22801 tacgttattt tattattaga ttattgacat ttatataata aacgattaaa tgattcatat
    22861 caatagaata cttaggaaaa aaagagaaat aataacaggt ttatgcacta ttgacggtgt
    22921 gacaatatga tatttgtatc cgtttttttt cttgtatgta gtatagtaaa gtagatacag
    22981 gaggagaaaa tatgcagact tttttaacaa aagtttctca aaagaaaatt aaaatgattc
    23041 atttactgct tgaaacagaa cgttggtgtt caatagaaga actacaaaac gaattgaagg
    23101 tatcaagtaa gtctatcttg aattatctaa cagatttaga agaattgttc caaaaatatc
    23161 ctgataaggt cattttgaag aatgagaata atcggcgctt ttctatggaa aagcaggaga
    23221 acttcccaat ctacattatt tatcttcatt tctacagaaa atcatataat tatcatttga
    23281 ttgaattcat gtatcaacat cctgagaaaa atttggaaga ttatgcagac gctcaatata
    23341 cgagtgtatc cacagttttt cgttatgcta agttattagt aaagtacttt gaaatgacgg
    23401 acgtcaaaga atagtagccg ccagatagaa ggaaattagc agttgaaaaa gctgccctgc
    23461 atcaagtaat acgaccagcc aaacggaaac agtgtctcat gtttttgatt tggctggtct
    23521 tattaccgca gatgcaagct tttgccacac ctctttcttg tcatttacag actcaactat
    23581 caatgctgca agctttccct taatgctgta acaccagtaa gagttgtcaa gaccagcaga
    23641 ctgactaggt aaataccgct caaaaacaag attacggcag ttaaactata atgatccatc
    23701 aagaaaccga tgacgataga agaaaaacca ccaacagctc gtccaaggtt cataatgaaa
    23761 ttattcgccg tcgcgcggat ttctgttgga tacaaactac taatcactgc gccatatcca
    23821 ccatacatgc cattgataaa gtagccgact atcacagcgg ctgctaccaa actccactgg
    23881 ctatgggcta acacaagtag atagacagat aaggatgaac aaattaaaaa caaagaaaag
    23941 gctagtttag gccccaattt atccatgatt atgccaaaag tcatcatccc caaagacatg
    24001 cctaaaatag tactgaccat ccacaatgaa gacccagaaa ccgaaagccc taatcgattt
    24061 tgcataattg aaggcagcca attcattaac ccaaaatagc cggcaatttg gacagttacc
    24121 atgatactaa ggccacatgt ttgccaagca agatgttttg attcaaacag tctgctaagt
    24181 ttggttttct tttcggcaga ttgtacagat ttgaattcct ttggttcagt caagccgcga
    24241 cgaatgaaca agaccatcaa caccggcagt acacctacga catacaacat cttccaacca
    24301 aacgttggaa tgatcacagc agctaaaatc gcagccaaag ccgcaccgac ctgcgcacca
    24361 ataccagcaa ccgaagaagc tcgtccaatt tgtttttttg aaaaactttc ggaaatcaag
    24421 gacatacacg ccccgtattc tcctcccgct cctacacctg cgataaagcg aaagagataa
    24481 accaagtaga tgtttgaagc aaaatacatc aataaagaag caatcgaaaa cagaaggacc
    24541 gtttgagaaa atactttcac acgtccatat ttatcagcta tgatgccaaa aaaaatacct
    24601 ccaatgagca tccccaaatt agtgatcgtt gaaatgaaac ccgcttgcgt accactaata
    24661 tgaaaagaag taatcattga agataatgaa aaagctaaaa acataatatc catattctct
    24721 aaagcaattc ccgccgatgt cgataataat aactttcgct gatacgttgt catttttata
    24781 ccccttttcc taaaatttcc ctaaattgtt aaaagtatat ccaaaaaaga ataaaaaaac
    24841 aagacttttt tatttgaaat aatacacaaa caaccgatat agataatcca gcttagtttt
    24901 aatagaaatg agtattgact tggagtacac ttaaagttgt agaataaata aaatacataa
    24961 gtaaagtggc aagaaattaa gagaaagagt gatcagatga atatcaaaca agtcagtgaa
    25021 gaaaagggca tttcggccga tacattgcgc tattatgaac gaatcgggtt gattccacca
    25081 gttaatcgga caaaaggcgg aatccgtgat tatacagaag aagacctaaa atgggtcgac
    25141 tttacgatct gtatgcgaaa tgccggatta tcgatcgagt ctttgattga atatattcgc
    25201 ctatctagcg aaggagaagc aactgtattt gagcggcgta agcttttgat tgaagaaagt
    25261 gaacttttga cgaaaaaaat tgctgaaatg caagaatgtt tggaacgttt acaaggaaaa
    25321 atcaagaaat atgatcgtgt gttatctaga aataaaatgg aatgtatcaa gtaaaaaaat
    25381 caaggagcat cgctatggag ttacgcttac taaaatattt ttggacaatt gcagaagaag
    25441 gaacaatctc tcaagccgca gaagttctac atcttacaca accaacctta agccgacaac
    25501 tgaaggagtt agaagaggaa ctaggaacag aactgtttgt acgcgaaaac cgcaaaatga
    25561 tcttgacaga agcaggttat tttctaaaaa agccgagcag aagaaatttt agatttaacg
    25621 aatcaaacca ctcaagaatt cgagaacaga aaaaaacaat tattcagcgg ccgatttacg
    25681 atcggctgtg tggaagctga taattcgaat acattggcac tattactaga aaagttgatc
    25741 cgtgattatc ccagcgttca atttcatatc cacagcggaa caagtgatga tatcacggag
    25801 tgactagaaa aaagcctgtt agatttagcg attttgctag atccagtaga tacagagaaa
    25861 tacgaaacga ttttgttacc aagaaaggaa aaatggggat tgctcatttt agaaaattct
    25921 gttttagcaa aaaaagacgt gataacaaaa gaagactttg cgaatgtacc tcttttgatc
    25981 tcaaagcgtc cagaaataca gcgaatgttt gctgactggt taggcataaa aaaggagcag
    26041 ttgctgatca caggaacgta caatttgatt ttcaatgtat ttgcgctaat tgagaatcag
    26101 gtaggtgctg ctttagtcat agaaggtgct gtaagtcatc tttcaaagcg agtgacattc
    26161 gttccgcttg acccaatgat tgaaacgaac tgcatgctag tctggaaaaa gaatcggatt
    26221 ttatcacctg tagtcaatga attgattcac cgtttcaaag aagcttttga acagtaataa
    26281 gtatctctaa tcgaagaaat ttcaatacaa aagcgagacg tatgacagtc ttgcttttgt
    26341 attttttcat ttttaaatca aaaaaaataa agagatttac ataacttgtt aaataagcgc
    26401 ttacaaacat aagaagaacg gaaatgaatc agctataata accgttgttc tggattgtaa
    26461 tcattacaaa gaaatgaaga ttataacttt ggaggtgttc gaaatgaaag aaaaaaagca
    26521 aaaattcgtt caaaaaacat gccctttatt caatggttgg gtatgattct gctgattgta
    26581 ttagctttat tgacgatcat tcctttttta tggatggttt ctgcttcatt caaagcaaat
    26641 aatgaagtgt tcacgattcc gattcaatgg atccctaaaa cttggcattt agagaattat
    26701 ctggttattt ggtctcgtat tcctttagtg acatttttta agaatacagc ttttttaagc
    26761 gttgtgatca cgttgattca gttgtttact tctagttttg ctgcttacgg gttttctaaa
    26821 ctaaatttta aagggcgcga tattttattt ttagcatata tcggcacgat tgctgtccct
    26881 tggcagtctt atatgatccc gcagttcatt atgatgagaa aagtgggttt aacatatacg
    26941 ttgtggtcgt tggtgctatt acaagcgttc aatgcttttg gtgtgttctt gttgaagcaa
    27001 tactacagta gtatcccaga ttctttatgt gagagtgcgc ggatcgatgg cttatcagag
    27061 tggggaatct atcgcaagat cattttacct cttacaaaac cggcgattgc cagtttgacg
    27121 atcatcacat tcgttaatac atggaacgat tatatggggc cattcattta tttgtcttct
    27181 acggaaaata aaacaatcca gttagggttg aaaatgttta ttgggctatt tgatgcagag
    27241 tacgcattaa tcatggctgc ctcagttgtt tctgttcttc ctgttgcttt tatattttta
    27301 gcgatgcaaa aatattttgt agaagggatc gcttcatcag gaatgaagga ataggaggtg
    27361 attatgaaag agcagcgttt tgacaaaaat gtttatatgg aattctttta ttggttgtat
    27421 caatcattgg caagtagcct ttgtttttgg tgtgtcaata tcttgtatat agcagctgcg
    27481 ttccttttgt cgatagatgg aaggaatgtc ttttccttta ttttttcttt actttttatt
    27541 ggtccaggtt gcatcgtgat gataggtatg acagaagaat tagcaaaaga aaaaagtttt
    27601 tcaccagtac gtttgttcat aaagacgttt cgccaatttt ggctaaaggg atttttttat
    27661 tggctatttg cttggatagt tagtgtgata atgatttttg attgtttctt ttttatacgt
    27721 ttttcttatg gaaaatggtt gattccgttg tttgttttac ttgcttgttt atcagtttcc
    27781 tttagcatca actactggta ttttcaggtg agaaatccag cgtcgaaacc aaatcaagtt
    27841 ctacggatag cgttctacta tacgttgaaa aaatggtatg tgagtttact agatttttta
    27901 ctattgacaa gtttgtttct cttctttttc gtaaagcccc aatggtgtat tctactaggt
    27961 ccttctatcg tatttggttt gatttacttc aacaatcgta aattgatgag aactatggat
    28021 ctgtagactt tataaattga aactttcaat taagaatgac agtttttatg taaaaactgt
    28081 cattcttttt cattttttga aacatatggc taaacaaatg aaataaaacg tgctatactt
    28141 ttgaatgaaa caggtttaga aactgtatca attatttgtg attatttcta actaataatt
    28201 aaaaataaag cataaaaagg acgatgttca atggacgaaa aaatgaaaca aggttcgaaa
    28261 tctgtagtag aaagtttgat taatcatcat gttgattatg tattcggtat accaggtgca
    28321 aaaatcgatg gtgtatttaa tgaattagag gatcaaggtc ctgaattaat cgtgactcgt
    28381 cacgaacaaa atgctgcatt tatggcacaa gcaatcggac gtattacagg agagccagga
    28441 gtagttatcg caacaagtgg tcctggtgct agtaacttag ctacaggttt ggtaacagcg
    28501 acagcagaag gtgacccagt tttagcaatt gccggacaag taaaacgaag cgatctattg
    28561 aagttgaccc atcaaagcat ggataatgca gcactgttcc agccaatcac aaaatacagt
    28621 gcagaaatcc aagatccaga aacaatttca gaagtgattg ccaatgctta tcgtatggct
    28681 aagagttcaa aaaaaggcgc gagctttatc agtatcccgc aagatgtagt ggatgcacct
    28741 gtccaaggga atgtcatcaa gccattgagc gatcctaaat taggttctgc ttctgcagat
    28801 gatatccgtt acctagctga acgcattaga gaagcaaaac taccggtact attagtaggt
    28861 atgcgaggat caagtgaaaa agaaacgtta gccatcaggc aattagttgg aaagacagca
    28921 ttgcctgttg tagagacatt ccaagcagct ggagtgatct cacgtgaatt agaagcgcat
    28981 ttctttggtc gtgtgggttt atttagaaac caaccaggag atatgctttt gaaacgaagt
    29041 gatctagtta ttgctattgg ctatgatcca atcgaatatg aagcaagaaa ctggaatgcg
    29101 gaaaaagatg cgcggatcat tgtgatcgat gaagcgccag ctgaaatcga tccttttatg
    29161 cagcctgaaa gagaattgat cggtgatatc tcagccactc ttgatttatt gacaggttca
    29221 ttagagcctc aacaagtgtc agaagatgca aaagaatatt tagcatctct tcaagcagaa
    29281 ttaacggagc gggacattgt tcaatcaaaa ggagaagcag gtatcttgca tcctttagaa
    29341 gttattaaca cgcttcagtc aaaagtaaca gatgatatga ctgttaccgt agatgtaggt
    29401 agtcattata tttggatggc acgccacttt agaagttatg aacctcgtca tttgttattc
    29461 agtaatggta tgcaaacgct tggtgttgca ttgccatggg cgatttctgc tgcattggtg
    29521 cgtccaaata cacaaatcgt atcagtttcc ggcgatgggg gatttttatt ctctgcccag
    29581 gatttagaaa cggcggtacg taaaaaattg aatatcgttc atttgatttg gaatgatgga
    29641 cactacaata tggtagagtt ccaagaaaaa atgaaatatc agcgggcgtc aggtgtagac
    29701 tttggtccag ttgattttgt gaaatatgca gaagcttttg gcgctaaagg aatccgagct
    29761 acaagtgtag aggaattaga aaaagcttta gaagaaggat ttgcgacaga aggacccgta
    29821 attattgata ttccgattga ttaccgtgat aatgagaaat tgggagaaac aatcttacca
    29881 gatcaattct attaaggaag tgtcgacatg actgaaaaaa tcttatacca gcacgggaca
    29941 ttaggagctt tgatggctgg cttgatggat ggaacagaaa cgattgctca tatattagaa
    30001 aaaggaactc tgggattggg aacattgcat gggcttgatg gtgaagtcat ctttttagat
    30061 ggcgtagctt atcaaggaag atcagatggc tcagttgccc aattagatgg aagtgaactt
    30121 accccctatg cagcaattac agattttacg ccagatacta gtttttctgt ctcaaaaact
    30181 gcagatggag aacaattgaa aaaagacata ttagtaagag aagccgggga aaacctattt
    30241 ttggcagtga agataactgg attattcaaa aatatgcaca tccgtatcat gccgaagcaa
    30301 gaaaaacctt acagaagact agcaaaaatt tcagagtctc aaccagaatt ccaacaaagc
    30361 aatgtccaag gaacattagt cgggttcttt acgcctgaat tattccaagg agttgctgca
    30421 gcgggatttc acttgcattt tatcgatgat acacatacat ttggcggaca tgtaatggat
    30481 tttgaagtgg ctgaaggaaa agttgagatt agccgcatct cgtcccttgt acagcatttt
    30541 ccagttgatg atccgacttt tgttcacgaa acgattgatt atgctgattt agcagcagaa
    30601 atccaacaag cagaatagaa aaagacagcg agatgcgttc aaggtgatta acctgacgta
    30661 tctcgttttt ttgaggagga aacctagcgt ttacaaaaaa actgagctat aatcttctcc
    30721 atagatcaaa aaggagcggt ttttatcatg tggacagaaa aacagaaaaa agatgcatac
    30781 gaaattttat tattacagca acaaggatta ttagaagcag aagataactg gttagcaaat
    30841 ttagcaaatt catctgcatt gttgaatgag actttgcctg agactgtctt tgcaggatat
    30901 tatttattcg atggtaatga attgatttta ggaccgttcc aagggcgagt ttcttgcaca
    30961 cgtatcaaga tgggaaaagg agtttgtgga gaatcagctg aaaaacaaga aacattgatc
    31021 gttgatgatg tcaaaaccca tgcaaattac atttcctgcg attcagccgc acaatcagaa
    31081 atcgtagtgc caatggtaaa agaaggtaat ttaatcggtg ttttagatat cgattgcgga
    31141 gtaacgaaag ggtatgatga aatcgatcaa gcatttctag aaaaatatgt agatctttta
    31201 ttagcaaatt ttaatataaa tgctttttca tttattaata actaaaatcg tctattgaaa
    31261 caaaaaagaa gagaaatcaa gaataaaaag agccggaaag acagtgggca tttctgtttt
    31321 tcggtttttt tattttttta taaaatcaca agtgttattg attttcataa tcatatttta
    31381 atggtattat cattttaaat aatggaaaaa cattaaggat ggatgaaata tgaataacga
    31441 aactatcatt ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac aaaaagtggt
    31501 tggagaagta atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg aatatcatga
    31561 cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt
    31621 ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt
    31681 agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc
    31741 actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag
    31801 agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga
    31861 actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat
    31921 ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat
    31981 ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga
    32041 agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt
    32101 accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc
    32161 tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc
    32221 tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga
    32281 attcttttca ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta
    32341 ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata
    32401 catatatgga gagggtgata tcccagttca aaccagatac attggttacc atctgtatta
    32461 acgattatgc ctctaaacgc tttttttatg aaaaaattta taagtgaaaa cgtaggtatg
    32521 atattatcta gaggtaattt ttttgggtat ttaaatagtt gctaggagga tag
//
LOCUS       DAZKCZ010000023        32168 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000023 DAZKCZ010000000
VERSION     DAZKCZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.023"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..340)
                     /locus_tag="ACG8KN_000925"
     CDS             complement(<1..340)
                     /locus_tag="ACG8KN_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9188002.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEY"
     gene            complement(358..678)
                     /locus_tag="ACG8KN_000926"
     CDS             complement(358..678)
                     /locus_tag="ACG8KN_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9188003.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            977..1753
                     /locus_tag="ACG8KN_000927"
     CDS             977..1753
                     /locus_tag="ACG8KN_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP9188004.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1768..1971
                     /locus_tag="ACG8KN_000928"
     CDS             1768..1971
                     /locus_tag="ACG8KN_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9188005.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1987..2325
                     /locus_tag="ACG8KN_000929"
     CDS             1987..2325
                     /locus_tag="ACG8KN_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFP9188006.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            2312..2491
                     /locus_tag="ACG8KN_000930"
     CDS             2312..2491
                     /locus_tag="ACG8KN_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188007.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(2534..3004)
                     /locus_tag="ACG8KN_000931"
     CDS             complement(2534..3004)
                     /locus_tag="ACG8KN_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188008.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3091..3789
                     /locus_tag="ACG8KN_000932"
     CDS             3091..3789
                     /locus_tag="ACG8KN_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFP9188009.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            3967..4308
                     /locus_tag="ACG8KN_000933"
     CDS             3967..4308
                     /locus_tag="ACG8KN_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188010.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            4301..4972
                     /locus_tag="ACG8KN_000934"
     CDS             4301..4972
                     /locus_tag="ACG8KN_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFP9188011.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            4978..5664
                     /locus_tag="ACG8KN_000935"
     CDS             4978..5664
                     /locus_tag="ACG8KN_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFP9188012.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            5667..6416
                     /locus_tag="ACG8KN_000936"
     CDS             5667..6416
                     /locus_tag="ACG8KN_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9188013.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            6428..6697
                     /locus_tag="ACG8KN_000937"
     CDS             6428..6697
                     /locus_tag="ACG8KN_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188014.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            6702..6866
                     /locus_tag="ACG8KN_000938"
     CDS             6702..6866
                     /locus_tag="ACG8KN_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188015.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            6859..7161
                     /locus_tag="ACG8KN_000939"
     CDS             6859..7161
                     /locus_tag="ACG8KN_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188016.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            7158..7319
                     /locus_tag="ACG8KN_000940"
     CDS             7158..7319
                     /locus_tag="ACG8KN_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9188017.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            7316..7621
                     /locus_tag="ACG8KN_000941"
     CDS             7316..7621
                     /locus_tag="ACG8KN_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188018.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            7621..7977
                     /locus_tag="ACG8KN_000942"
     CDS             7621..7977
                     /locus_tag="ACG8KN_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFP9188019.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            7937..8182
                     /locus_tag="ACG8KN_000943"
     CDS             7937..8182
                     /locus_tag="ACG8KN_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188020.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            8179..8598
                     /locus_tag="ACG8KN_000944"
     CDS             8179..8598
                     /locus_tag="ACG8KN_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFP9188021.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            8595..9152
                     /locus_tag="ACG8KN_000945"
     CDS             8595..9152
                     /locus_tag="ACG8KN_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFP9188022.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            9149..9445
                     /locus_tag="ACG8KN_000946"
     CDS             9149..9445
                     /locus_tag="ACG8KN_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188023.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            9522..9935
                     /locus_tag="ACG8KN_000947"
     CDS             9522..9935
                     /locus_tag="ACG8KN_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9188024.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            10244..10396
                     /locus_tag="ACG8KN_000948"
     CDS             10244..10396
                     /locus_tag="ACG8KN_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188025.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(10393..10668)
                     /locus_tag="ACG8KN_000949"
     CDS             complement(10393..10668)
                     /locus_tag="ACG8KN_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188026.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            11164..11328
                     /locus_tag="ACG8KN_000950"
     CDS             11164..11328
                     /locus_tag="ACG8KN_000950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188027.1"
                     /translation="MPATLNAYDGYDYRKKIVKKLYGATYGNVVSVLSVHHQASVTVA
                     EVEDAAVNGE"
     gene            11524..11691
                     /locus_tag="ACG8KN_000951"
     CDS             11524..11691
                     /locus_tag="ACG8KN_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9188028.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            11717..12061
                     /locus_tag="ACG8KN_000952"
     CDS             11717..12061
                     /locus_tag="ACG8KN_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP9188029.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            12066..12347
                     /locus_tag="ACG8KN_000953"
     CDS             12066..12347
                     /locus_tag="ACG8KN_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188030.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            12450..12764
                     /locus_tag="ACG8KN_000954"
     CDS             12450..12764
                     /locus_tag="ACG8KN_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFP9188031.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            12742..14436
                     /locus_tag="ACG8KN_000955"
     CDS             12742..14436
                     /locus_tag="ACG8KN_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9188032.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            14456..15634
                     /locus_tag="ACG8KN_000956"
     CDS             14456..15634
                     /locus_tag="ACG8KN_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9188033.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            15597..16283
                     /locus_tag="ACG8KN_000957"
     CDS             15597..16283
                     /locus_tag="ACG8KN_000957"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP9188034.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            16283..17443
                     /locus_tag="ACG8KN_000958"
     CDS             16283..17443
                     /locus_tag="ACG8KN_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9188035.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            17453..18328
                     /locus_tag="ACG8KN_000959"
     CDS             17453..18328
                     /locus_tag="ACG8KN_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188036.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            18325..18636
                     /locus_tag="ACG8KN_000960"
     CDS             18325..18636
                     /locus_tag="ACG8KN_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188037.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            18626..18979
                     /locus_tag="ACG8KN_000961"
     CDS             18626..18979
                     /locus_tag="ACG8KN_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9188038.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            18969..19370
                     /locus_tag="ACG8KN_000962"
     CDS             18969..19370
                     /locus_tag="ACG8KN_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188039.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            19363..19767
                     /locus_tag="ACG8KN_000963"
     CDS             19363..19767
                     /locus_tag="ACG8KN_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188040.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            19779..20387
                     /locus_tag="ACG8KN_000964"
     CDS             19779..20387
                     /locus_tag="ACG8KN_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9188041.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            20407..20769
                     /locus_tag="ACG8KN_000965"
     CDS             20407..20769
                     /locus_tag="ACG8KN_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188042.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            20772..20954
                     /locus_tag="ACG8KN_000966"
     CDS             20772..20954
                     /locus_tag="ACG8KN_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188043.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            20971..24402
                     /locus_tag="ACG8KN_000967"
     CDS             20971..24402
                     /locus_tag="ACG8KN_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9188044.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            24453..25190
                     /locus_tag="ACG8KN_000968"
     CDS             24453..25190
                     /locus_tag="ACG8KN_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9188045.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            25200..27491
                     /locus_tag="ACG8KN_000969"
     CDS             25200..27491
                     /locus_tag="ACG8KN_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFP9188046.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            27515..29641
                     /locus_tag="ACG8KN_000970"
     CDS             27515..29641
                     /locus_tag="ACG8KN_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188047.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            29658..29807
                     /locus_tag="ACG8KN_000971"
     CDS             29658..29807
                     /locus_tag="ACG8KN_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188048.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            29804..30250
                     /locus_tag="ACG8KN_000972"
     CDS             29804..30250
                     /locus_tag="ACG8KN_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188049.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            30252..30389
                     /locus_tag="ACG8KN_000973"
     CDS             30252..30389
                     /locus_tag="ACG8KN_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP9188050.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            30427..30720
                     /locus_tag="ACG8KN_000974"
     CDS             30427..30720
                     /locus_tag="ACG8KN_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188051.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            30717..30941
                     /locus_tag="ACG8KN_000975"
     CDS             30717..30941
                     /locus_tag="ACG8KN_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9188052.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            30938..31963
                     /locus_tag="ACG8KN_000976"
     CDS             30938..31963
                     /locus_tag="ACG8KN_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFP9188053.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT        11492 a         4909 c         6695 g         9072 t
ORIGIN      
        1 aatactccat aaatttttcg agattaaact gagattcatc agtcagttcg ttctcaatat
       61 agatatttaa taaaattaga atagctattt tatctgcttc tgtttcaaat tttgagtgaa
      121 aagttgtaga agtatcgtac aaaactgaaa attcaaaatg agaggcgctg aaatgtgcaa
      181 gttcatgaga taaatgaaag gcttctgcag tttcaccgta tagattttca ttcaaaaaaa
      241 tgattctggg ttttggatag tagaaaccag gctctttcat ctccatataa actactttca
      301 aattgtattc gctcaacatt tctttcaatt tcaaatacat acaaaccatc actccaacta
      361 ttcattttcc tctaaagctt tagcaattgc aatcgcttta cgcattgtct ccttagatat
      421 ttcttttcca tcaaaagaaa aaacagtatc gtcttctgat aaatccacat gtttaggggt
      481 ttctctttct tctctaccta gaaggtagtc tacagagaca tcgaaatagt cagcaatccg
      541 acttaattca tctgaattag gagtattatt tttccacttt gctagataac cgttagaata
      601 tccaaaatta atttctaact gccgtataga tatctttttc ctcttcgcca agtcttttat
      661 tatttcatag gtattcatcg ataaaacaac ctttctaaat gtttacaaaa aaagtttaga
      721 aaaatacgca gaaataattt gactaattct gagtaatgag ctatactata tcttgtaaac
      781 agatttaaca actaaaaaga caacaaaaaa cactattgat atgtaaatgc agaccgccaa
      841 gaaagcttta aaatcaatgt ttttatgtct tatttaatta tgctcttagt atagagtatt
      901 actcagatac tgtcaattga atttagaaaa aagttgttaa atttgtttac gaatataaaa
      961 gaaaggaatg agaaatatga acacaccgca aatttttaat ttcgaacaaa acgaagttcg
     1021 gacaatttta gtaaatgatg aaccatattt tgtaggcaaa gacgttgcga gtgttttggg
     1081 ttattcaaac actaaagatg ctttgtcgcg gcatgtagat ttggaagata agatggggtc
     1141 gcgaatcacg acctcaggtc aatcaagaga gatgacaatc atcaacgaat ctggtttgta
     1201 cagtttaatc ttaaaatcaa aacttccctc tgccaaaaaa tttaaacgtt gggtaacaag
     1261 tgaagtgttg ccagcaatta gaaaacatgg aggttatcta actccagaaa aagtagaaga
     1321 agctttgctt aatccagata caatcattca attagcaact caactaaaag aagaaagaac
     1381 tggaagatta atcgcagaac aaaagattgc agagtacgaa cctaaaatct cctatttaga
     1441 tagcatatta tcttctacag attcagtaac aattagtcag attgcagcag attatgggat
     1501 gtctccacaa cagatgaata aattacttca taaactaggt attcagaaaa aagtcggtaa
     1561 ccaatggtta ttgtgcaaaa aacacatgaa ccaaggatac acaaaatctc atacaactga
     1621 gatcccgaaa gccgatggtg gcactaaaat tgtaatgaat accaaatgga cacagaaagg
     1681 gcgtctattt atctacgaat tactaaaaaa agaaggatat taccctcaaa tggatttaga
     1741 ggaaattggt tagaaaggag ttttagtatg actgacattg cagaaatcac tcaacgagat
     1801 agagaaaaaa tcaaagaata tgtcgaaagt tcaaagttct taacttacac catgcttgct
     1861 gaaagatttg gaattagcaa aagctactta tctttaattt taaacggtaa aaagacttct
     1921 gcagaagcaa acagaattat agattcgatt atcactatgt acgaattgta aagggaggaa
     1981 cagctaatgc aatatctaga agcaaaaatc ccaattccag aaggctatgt aattatctcc
     2041 caagtggatt atgaggagtt aaaaaaagct gatgatactg gtagatggat gacgttgcca
     2101 gaagtgctag aacggattaa cagaaaatat gattggttta cttctagagt tttaaagaac
     2161 ccaagatata gaaatattat cgatatcgag aaaaacaaaa atggatttgt ctattatcca
     2221 gttgaaggaa gagacacata tctattttta agaagtaaaa cacttgaatt tttagaaaca
     2281 aatttttcgg aaatcttaag gaggcaagcg gatgggaaaa tttaatagag cgctagtgtt
     2341 cagcgcaccg ctaatcatct acgctttagg actttgggga agcaggcaag cgttgatagg
     2401 aacgattgtt tacatggttt ggatttttat ggggcttgat gaagctgaag ctgagtacag
     2461 agcgaaaaag ccaaccgagg gggctgacta aaaatgaata gaaaagagaa actagaatgt
     2521 atattactat tactcagttt aattctttca ctaatttctc tattgggaag tttgtatttt
     2581 tgatatcaac aattttgtgg atttgggttc tatttagagt aaatatcatt tttgtttggt
     2641 gattaaggag tttattaaaa ttaattttcc ctacttcaaa tgcaaggtaa caagatatag
     2701 cacttttagg aggaatattt agcggtacag cttcagagta tattctatta tcatcgactg
     2761 atcctccttt agcaattaat ttcttaaatg gagtagctga ccatctgcgg tttaagttag
     2821 cggatgaaag ttctaaatta actaacatag ctggttctgt tgaatagtta gaaatgatta
     2881 cttttgtata aatcatatct gcagcgaagt aagaagcatt aagttcaact tctagttgag
     2941 ggcgcattcc ttttattttt atccctgtta aaaacgtact caatataaaa cttattattg
     3001 acatccattg aaaaattgtt aagtgaaaaa aattcaaaat aaacaccacc agtttttaac
     3061 taaattatac caaaaaggag agaagaaata atgcaagaat tagtaatttt gaaaaataaa
     3121 gaagctgtga ctacgagctt acaagtggca gaaagcttcg agaaaaaaca tcaacatgtt
     3181 ttaagagata ttgatgcact aaaaaaagat gtgtccaatt ttggacagat gtttgtagaa
     3241 ggtaatgaac cagattcata tggcagaaat cgacgagttt tcttcattag tagagatgga
     3301 tttttcttgc tggctatggg gtttacagga aagaaagcta tctacttcaa acaaaaatac
     3361 attgaagcat tcaacgaaat ggaagatgtt attcgcaaga atactgttcc tcaaacaatt
     3421 gaagatatga tgatctatca attagaagaa atgaaagatg ttaaaaaaga tgtttccatg
     3481 cttaaagata ctatgcgaat tagcggacaa caagagtttg aaattaagca aaaaggaaat
     3541 atgaaagtta tgggagttct agggggaaaa gaaagccgag cttatgaaga aatcagcaaa
     3601 aaagtattct caaaattttg gtctgaattt aaacgtacct tttcaatccc aagatatggc
     3661 gagttacctc gtaagagatt cgatgatgct gtttcattta ttgaaatgtg gttgccagaa
     3721 actgcgatcc gtatggaaat tgatcaactg aacagacaac agagactttt tggtgatgac
     3781 aatgaataga gctgaagcgc taagaatagg gacggcaatt gctaattgct ggtggaaata
     3841 ctataaacca atcatcctaa gccaacaaca tattgacaag caaaaagcat ggcaacaaat
     3901 aaaaaagtga ctccgccggc aagcaaagag tcacaaagaa aacacatcat aaggagattt
     3961 tagcatatgg aaaaagaact ttccactcta gatcaatatt tgattgatcc tgattggggc
     4021 aagccgaaaa ttgaggaaac aagtggtcga aaaatcagac gaaatctttt gacgaatgaa
     4081 gaactagctt gggatcaaga tgatttaggc aaccatgtaa ctatttggga tcatgtttat
     4141 cttatccatc tatcgaagca ttcgaataaa cctgaatata tttacgtcat cgaagatggc
     4201 ttgattgatg cgctagaaga gtacgacaga gataacttga ttgatatctc ttattacgga
     4261 ccaggtaaga aatacattgc tgaaatggag gcagaatttg atgagtgaaa tcaaagggac
     4321 aacgaacttt gaaaaacttt ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa
     4381 ttttgattat ttatcttggg ctcacgcgtg ggagattatg aaaaagaatg atccacaggc
     4441 aacggtaact attaatgagt acaaacacta cagggttgtt tctggaactc atcaagactt
     4501 tcttgttgag gaatataaac cttttcttat ggatgaaact gggacttatg tatctgtctc
     4561 agtaacggtt aaaggacaca cggaaaccga attatttcct gttttagatt atcgaaacca
     4621 accagttgtt aaaccaaatg caatgcaaat caataactca ttgaagcgat gctttgtgaa
     4681 agcattggct ctacacggac tgggattata tgtatttcaa ggggaagata ttccaacacc
     4741 acctagaatc gatacaaaga aattaaacat gctagagacg attctagaag ctttcaatga
     4801 gcagatgggt aaagatatga ccaaaacctt aatcgaatat gttaatgagc agacagataa
     4861 attagggctc ttagctgata acgttgaaac tattgaacag ttaagctatg agcaatgtgc
     4921 cttgatggag cgagcaatag cagctaagag aaaagaatta gataagaagt gatatgagtg
     4981 tttaaaccat taatcgattc atattcagcg gttctgaaaa agttcaaagg aaaagacata
     5041 agcgcaacca tcaatgagga agtgaacatt gatcgactaa agacgatgta tgacggctac
     5101 gatggtgatc gagtcattga aattcgtttt attgatccta gacgtttcac cgtacagcaa
     5161 cgaaacttca tctatgcgct gataggcgat atttttatcg atacaggcat gccaacggac
     5221 ttctggaagg aattcttcta ctttcgtttt gaaggtgtca cagggcgcaa aataagcctg
     5281 aaagacgaat cgaatacaac tgtgagtgat gccaatgtct tagcaaatat catcttagat
     5341 ttcatctttg aacatcatat tcctttcaaa gaaggctatg agattttacc tgcgaatcaa
     5401 gaatattact tctacaaatg catcacaaaa agagtctgct gcatctgtgg caaaacagga
     5461 gctgacatcg atcactttga caaagcgcta ggaagacgaa agcgcaaaga agttgatcat
     5521 gcagagtaca catttgcagc actctgcaga atccatcaca cagagaagca caaaataggt
     5581 gtgattaatt tcaaaaataa atatcaaatc aaagggatca aattaaacca gaaaacaatc
     5641 aaaaagttaa atattggagg gtaaaaatga cagaacatcg aagttattat gcgattatac
     5701 cagccaacgt aaggtacgac aaaagactta aaccaaatac taagttgtta tacggagaga
     5761 taacggcctt gtgtaatgaa agaggctttt gttgggcagg caatgagtac tttgcagatt
     5821 tatatggtgt gaataaagag accatatcgc gatgggtaag tgatttgatt aagtttggat
     5881 acttgaatcg ggaaatcatt tacaaagagg gtaccaatca aataatcaat aggtacctac
     5941 gaattaatca ataccctatt gacgaaaaac gcaatacccc tattgacgaa aaagtcaaag
     6001 ataataatac atctattaat aatacattta ataatacaaa agaatatata agagagttac
     6061 cgccttcgaa aaaatcgaag gctaagcccg tccgtcataa atacggagag tataaaaatg
     6121 ttcttttgtc agatgagcag atggagaaac tcaaaacaga attccctaat gattaccaag
     6181 agcgaatcga acgactgtca gagtattgtg aatcatctgg taagacttat aaaaactatt
     6241 tggcaactat tcgaagttgg gcaaggaaag aaaaaagtga acctaagaac gcaagcagtg
     6301 gatacaagcg cacaggaaga cgagagaagc ttcctgaatg ggcaatcgac caagaagcct
     6361 atctcaagaa aaaagcgcta gaacgagcta atagacaatc aaaagcacca ttctaagagg
     6421 tggaaaaatg aagatcgatt atctagaact aattaatgaa atagcgaatt ataaaaaggg
     6481 cgaggaatta gacgtcctga gagacgtata tgatcaactc gaagaagctg gaattgaagg
     6541 aattaagaat gatcgttcga gttggagtaa gctcagatac tatttcgcac tttatatcga
     6601 tacaacacaa ttaagaaatt tagcatatac caaattacta tttgttgatt gtgttaaagg
     6661 cttgcaaaaa catcttaatg aacttgagca ggtgtaatca gatggaccta aagacattta
     6721 cagcacagat tgaactaatg catcaagaag ctttaagaca aagtgtatcg tacgaagaca
     6781 agtggctcaa cacgttccat ggcggacgtg agagcgcact tgatcaagta ctcaaattac
     6841 tgaaaggaga atgtcgggat ggataagaaa gcggcaatgc agcgaattat cgaattgact
     6901 tattcagaag attggcaaaa tgacaaagaa gctgcttcag aagtgatgag gcttggaaga
     6961 gcgatgtggg cagacaagag caacaagcca agaccacgaa aaatcgcaat ttggcacggt
     7021 gacaaacttc tagtgatagg gacagctgaa cagttagcaa gtctcacagg cttgcacgag
     7081 aaaatcgtga ggaaaagagc aaggtgtggc tacacagacg ctaagaagag aacgtttaga
     7141 tacgtggagg aatcatcatg acaacagaag aagtgattca aatgcgtatt cgaagccttc
     7201 agcgtgagat tgacgatctg gaacgaacaa aggcagtgat ggtcaatgaa acggcgagaa
     7261 aggcaatcga tttgcacata gagaatttaa gaagggaaat ccatcgattg gaggaatgag
     7321 cgtggataag aaagcagcaa tgaaaagaat tgctgaatta accaagtcag aatcttggca
     7381 agaagacaaa gaaatagttg cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa
     7441 gcccaaacgg agaacgccga gaaaaattgc aatctggcat gatgaccgaa ttttagtaac
     7501 aggtactgct gaacagttat ctgaaattac tggattaagc aaaaacatta tctgggatag
     7561 agctaggagc ttatggattg attcaaaagg acgacagttt aggtatgtgg aggagagata
     7621 atggatctca ttacacaata cagtgacatc atcctcaaga aaatcatgat gaagattcag
     7681 aaagataaaa aatcaaaaga acgagctgaa ttagttaagt tagaaatggc tgaaacagga
     7741 gcaggagtgc gaagtagcag gcattggaaa gcagcagcaa acattgaatt ttattacaac
     7801 gaaattcaaa aagggttcga tcagatgcgt gagctggatc ggcaaacaaa ttggagcaag
     7861 aaacttcatc aagatcgttt caaatttgta gagaagtata aagaaatatt agaagagtat
     7921 ttgaggagga cagcaaatga taaaaaaact cgttcaattc agcatggatt tatatgatat
     7981 cgaatcagga gcaacactat ctgtggaatc ggaccatcta atcataaatt ttggtggaaa
     8041 gcgccagatt attttgtggg tagttgatga tgtactgttt ccagaaattg ttcatgattt
     8101 cgaagaatca aaagcggttg agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa
     8161 atacgaggag gacagcgaat gataccgaag tttagagcgt gggataaacg aaagaacgta
     8221 atgagagatg tagccgtctt gcattttact aaaaacggca aaacaaactt tattgaatat
     8281 tggataaatc ctaccgaatt gaaatcatat cacgtgcgaa acatcgacct catgcaatcc
     8341 acaggaatga aagataagaa tggtgtggat atatttgaag gggatatagt attagtcagc
     8401 gtgcgaaatg gcttcgatta cttagataat aaagtctgta ttgtcaaaaa ttcaatagat
     8461 tattccggat tagtttgtgc cactgttgat gaagacttag agtatcaaat ttttaacaca
     8521 gagctgtttg aagaatacac gtacgaagtc accggaaata tatacgagaa tagcgagtta
     8581 ttggaggaac agcgatgaat aaacaggaag caatcaaaaa gttagaaagt attaaagcga
     8641 taggaaatga tgcaatagct gcttgctata acgagagtat aaattcaggt attacgttaa
     8701 tgaaaaaaat agacgaaccg cagaaaccag ttattccaca actcgtggcc ggttggcttg
     8761 agaaatctac ggaccctttt acaaaagctg aaaaaatagc gtatttaatc aaatctaaag
     8821 atggtgattc atattatttc tgtgattggt ttgtacgaga tggcatagtg acgcaagagc
     8881 aaggagaaga attactcgct tgggcaacaa gacaatcata tgaaacacta ttgagcctat
     8941 acaacggcta cgaggttgag aaagagccgt tatgggcaat aaagaatgcc gatggaaact
     9001 atcttactaa atgtgcttta tggggaaaag atggagtaaa ttatagtttt gaatgcaatc
     9061 catctcatcg attgcttttc actgataaag caacagcgga tgctgcagca ttgttggtga
     9121 atggaacagt ggaagaggtg gtagaaagat gaaactaaaa gacggatttt acgctagcag
     9181 tcacggtatc ggcggtttaa tgctagatat gccgacaaag aaccctaaaa cacgcaagaa
     9241 atcaaaattc aaagtcggtg acatggtccg ctgtgaagca gaagagttcg tttatccgtt
     9301 cagaggatac gttaaaaaga tactgtcaaa ctcagcaatc attcgtattg aaaatacaat
     9361 gaagtgtgat cgttcgactg caaaacacaa gcattattta gctgtagcga gattagttga
     9421 catggaagta atcaagagca aataaaaaaa gccggatcgc tccgactgat tcaataaatc
     9481 caacacattt attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg
     9541 tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg
     9601 agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattataacg gatatgccga
     9661 aggcaccgaa gcatggcaat aaggcagaag acgcgattat tcagatgatg gatatagaaa
     9721 cggagagaga tgcgattcta gcggctttga tggctcttag tctgattagt cgtcagatac
     9781 tctactacag cttctgtgtg ccagatagct tctcaaacta cagaattagc cgtgaagtgg
     9841 gttattcaga aagaagtata caacggatga agtcggaagc tctaatagag tttgcagaag
     9901 catataaaca cggaaaaata attgcttata aataatttgg cggttttttg gcggaatgat
     9961 ggcggttttt agacatttac cagtgttatt atggtagtgt cgaaagatta ggaaacagga
    10021 tcgacaaaat aaacgaaggg aggcaatctc cctcatcgtt ttaaacttct tcttagatag
    10081 acagcaacta attatgaaga aaaggatgtg aattcaactc ctcctaaatt cttcatcggt
    10141 tgctgtttat tttgattaga cagcagcact cacaaaacta aacatgttct tgtcgtcttt
    10201 caactgctgc tgtctattaa tttatgtatt ggagggaaga aaaatgaaat tatcagtaga
    10261 aggcactcca gaagaaatac aagaattgct ccaagctatt ggtagtagca aggagcaaaa
    10321 ggtaagtgtt tctaattcag atattgacgg tatttgcaat tgtttaaaca gagaaaaaat
    10381 atctaatcct tactaagaga aaatatgttc gttaatgaat ttccaatttt cctctgtagc
    10441 tctaccttga taattttttt gcagttcaca gatgaataag gaatcgttat tatcaagtac
    10501 tgtatttaat ttttcacaca tttgatttgg attcaaatta gttttaacta accaaaaaga
    10561 tttttgtagc tttatatatg ccccaaattc ttttattatt tcaaatactt tatcatactt
    10621 ttgccctggg ttattaagat cataactaat catgtagggt ttatacatgt tgtcttgcac
    10681 atccttgttt tatatttcag cggaccactc gctgataaat aaaattatac gcttagtatt
    10741 tattttcaca atattaattt atcgtaataa cttttgagta aatagaacaa aaaacctgca
    10801 ctagtttccg ctagtacagg cagtgactat atcggttgat aatctagcat atattaaaaa
    10861 taattgcaag aaaagaaatt tatagtggtt tataccaaat tatcaaagca atgttatttt
    10921 gttgctgtct attgtttttt aattattcac acgataacta aaggtgggtg aagagaaatg
    10981 attccattaa taatttcaat ttttgcgctc tgtcttaatg tctatatgat tggatttaaa
    11041 aatgggcaaa ataaaaaata gtagcagcca agaataattt tatagtgtca ctgtggcgga
    11101 aagggtagac gctaagcatg tgtgctaggt caatgcttcg gcaaccatgc aaggttcgat
    11161 tccttgccag cgacattaaa tgcctatgac ggttacgact accgaaaaaa gatcgttaag
    11221 aagctatacg gtgctacgta cggcaatgta gtaagtgtgc tatctgtaca ccaccaagct
    11281 tcggtcactg tggcggaagt agaagacgca gcggtaaatg gcgagtagcc tcgtgagagc
    11341 ctggtaagtt ctcgtgagtg gtgcaatccc actccagcga ctttaagcaa ccgaggcatc
    11401 ggcggtttaa aaatataggg gtgcgcaatt tcgtacgcgt tttgtgcatc gtgcaagtta
    11461 ctattacata ttagatcact ctttgagtgg tctttttatt tttgcacaaa ggaggaaaca
    11521 acaatgtata gaccacaata cttagaacag aagtatgaag taatcactgt tcataaaggt
    11581 aatggcgaaa aagtatatga gtatagaaga ccaataaaga gcgatacata taaacgaaag
    11641 gaaaacaatg aagttattcc attgtatggc aaaagaatag ctaagcatta aataagattg
    11701 cgaaaggaga cggaacatga ccgaggaatt ctatagatgg ctattacagt tgacaagaga
    11761 agatcgtttg gttaagttct atcagtctcc taaatggcgc aggcttagag agaaagcgat
    11821 gaaacgagat cactatgaat gccaagagtg tagaagacta ggtaagtatc atagagtaga
    11881 gaacgttcat catataaagg aagtcaagga tagacctgac ttagctttag atttagataa
    11941 tcttatttgt ttatgtgttg aacatcataa tgaagttcat ggcagatatc ttacagcatt
    12001 agataaacaa gagaagaaga tagaaagctt tgctaacttc gatgcaagtg aaaggtggta
    12061 agtgcatgat catcaatgat aatggcagag agtatgatac agaaaagatt gaagagtatt
    12121 catcttatac tcagggatta attaaacgtt tgatatacgt tcgctatgta ggtattaggg
    12181 atctgttatc agataactgt tgtagtaaat acaaagtgaa tcaagtaaga gaagcgttga
    12241 ataaagataa taacgtcgaa agaataaaaa atgtttttgg atatagtatt gaagagatta
    12301 attattacat tgacttcgct gaagctttca ttccgatggt gagataaccc ccccttaaaa
    12361 taaatcgcaa attttttggg ggtgatgaaa cggagggggc tgtcaggaaa agagattttt
    12421 tcgaacttta tcatgaaagg agggctaaaa tgtttaaaaa cgaattgtct caaaatcgct
    12481 acagagaaaa attacgccgc tctttaataa gccaattgga aagtcagaaa acaaatattg
    12541 agccattctt agataatgtt gatcgttata tcagtttatg ggaaacggcg atatcactgg
    12601 aagaagatat atccgagaac ggcattagac tggagaatgg taaaaagaat gaatcagtag
    12661 cgttgcttgt ttctgtcaac aaacaaatgg gattgatgtt ggataaactt gccattactc
    12721 ctgaattggt aggtgaagca aatgaatcaa ttcctgagtt ataagcatat tgaaaattgg
    12781 ttcaaagcta tagaagaagg cactatcaag gtatgcaaag agcaattatt gctaaaaaat
    12841 tatctagaag aaagagtctt tactagagaa gatatttact tcgataagca gatggtagag
    12901 gattcaatca atataccagc acaatacttt ccattcgaat taattccgtg ggaaaaattt
    12961 ctacaatgtt ttatttatgg tgttcgatgg aaaaaagata aaacactagt gttcaataga
    13021 tatctttcat taatgggacg tggtaatggt aaaactggtt ttgcttcttg gaacaacttc
    13081 tttctactaa ccgctaaaca cggtattaaa aattatgata ttgatatcta tgccaataat
    13141 gaaagccaag caaagactag ttttgatgat gtatttaaag taattaaaga tcatcctgat
    13201 ttagataaaa aagtatttaa agctacgaag gaagttattc aaaatatcgc tacaaacagc
    13261 aaacttcgtt ataacacggc aaatgctaga acaaaagatg ggaagcgacc aggtgcaaac
    13321 cgctttgatg aaattcacga aaatgaagat tattcaatga taaatgtggc tacttctggt
    13381 ggtggtaaaa ttcgagatta tagagaattt tatgatacaa ctaatggtca tgttcgtggt
    13441 ggtccgcttg atgacattat agaagaatca aaaatgattc tttctggaga acttggaatt
    13501 gacaaggatg gagcagaatt ttctagtttg tttccattta tttgtcgctt ggataacgat
    13561 aatgaagttg atgatcccga catgtgggaa aaagcttgtc caactattaa ttacaatgca
    13621 gatttaaaac ggaaaatgtt tcaagaatac tctcaaatgc aacgtaatgc tggtttaaga
    13681 cttacgttca tgaccaaacg aatgaacaga cctatggaag atacacgatt tgctgttgct
    13741 tcatatgatg atgttctgca tacgaaagaa aaagaatttc ctgaaaaaat ggatgaagtg
    13801 ataggaacag tcgattttgc tgatagacga gattttgcca gcgttgggtt gctaggaaaa
    13861 tacgataaag atgtttattt tacacaacat acttttatcc acgaatcagc ccttcgatta
    13921 caaaacatca aacgagaggt tatagatatt tctatagatc aaggaaaatc acagatcgtt
    13981 catggaaaaa atatagaagc tgattatatt gtaggttggt ttcttgaaat gagtaataaa
    14041 tattatatta aaaaaatcgc tatggatatg taccgtgcaa aaatattgaa gcccgcttta
    14101 gaagaagcag gttttactgt ggaaattgtt cgaagcggat ctgttacaca tggtatgtta
    14161 aaagatctgg ttgatgacct ttttattaat caacgtttat tttttggtga cgatgcgatt
    14221 atgcgttggt attgcatgaa tgtatatgaa gagcatattt ctaatggaaa tatacgctat
    14281 gaaaaaatag aacctgaaac tagaaaaacg gatggctttt tttcattcct tcatggtttg
    14341 aattttttag atgatattta tgattctgct cctgtaacag tcacaaatag ctcagtagaa
    14401 aatacaggaa ctggatttac tcctctagta ttctaacttg aaaggaggtg agaaagtggg
    14461 gatttttcaa aaggcggtag gatacttcac aaaaaaagca acggttcctt tagaagaata
    14521 cttttgtaaa ttgcaagttg attttgtgta tcgaaaattt gcgattgaaa cttgtattga
    14581 tttgattgca aatgcgatga gtaaagcgga attcaagtca tatgaagatg gaaaaaataa
    14641 aaagaatgat ctttactata ggctgaatgt agctcctaat aagaaaaata atgcaacaga
    14701 atttagaaaa aaactgatca ggagattaat attctacaat gaagtattga tcgtttctcc
    14761 gtctaataat tctagcgaaa tatttattgc ggatagttgg gatgtcacag aatatgcatt
    14821 gaaagatgat gtgttttctc aagtgcaaat taacaacata gtccttgata gagaatttct
    14881 agaaagtgat gttatctata taaaatacgc agatcaacaa attaggcaac tagtcgatgc
    14941 gtattatcaa gcgtatggga aactcatttc tagtgccatg aatgtttaca agcgctctaa
    15001 cgctcgtaga tacgtactga aagggaattt attccgaccg caagacaata caacacaaga
    15061 tcaaatcaat aaaatgatga catcacaatt taaggctttt atggaagctg ataatgcagg
    15121 tgcggtattt caattacaaa atgagtacac attagaagat ttcagcggaa acttccaaag
    15181 caattcaaga gatataaaaa acttaataga cgacatcttt gagatgacag cagcagcgtt
    15241 tcacgttccg aaaaacctac taaagggaga catgagtggg ttatcggatc aagtggacgc
    15301 ttttttaatg ttcgaaatca taccgattgc tgaacttatt caggatgcgt ttaacgctag
    15361 tctctatgaa gtagaagaat acttgtcagg gaattttgta cgtgtggata caactatgat
    15421 caagattact agcttcaaag atttggttga cgctattgat gtaggcatta gaaatggagt
    15481 atttacaatc aacgaaggaa gagaacgcgt tggaaatgat cgctctgata aggcgatggc
    15541 agatgaaata tttataacta aaaacaatca acaagtatcg aaaggaggtg aggcgaatga
    15601 cgacaatgaa aacatttcta gcagtaaaga atgaaggcgc agtaccgcaa atttttattc
    15661 agggatttat tggttctagt tggttctttg aagggaatac tgacaaggga atcaaaaata
    15721 ttttggatag tctaggtgat caagaagaaa ttgaagtagt aattaattca aacggtggag
    15781 acgtatttca agggattgct attgggaact tacttaagtc aaataaagca aaagttaacg
    15841 ttgtgattaa cggcttagcc gctagtgctg cttcaattat cgcaatggct ggcgatacta
    15901 taaaaatcta caacaatgca caattgatga ttcaccgcgc ttccacatac ggagaaggaa
    15961 atgtcgatga cttccgtacg attgctgacc aactggaatc aattgataaa tcggtaaagg
    16021 cttcatataa aacacgattc aatggcacag atgaagcatt gcaagaactt cttgaaaaag
    16081 aatcgtttat ggatgcagaa acagctttga gttatggatt ggtcgatgaa attatcgatg
    16141 cagaaaatag ctcaggtact gaagctaaaa aagaacaaag cgttgaagaa attttgaatg
    16201 acgttaaaga aaaaagagca gaaaaaattg ctgcatttac agcagcatta aataaaacat
    16261 ttggacaagg agatgtaaaa taatgacagt taaaaattta aaaggtgtaa cagctgcaag
    16321 cgaccaattg atgaaagctt ttaaagatgg taacgaagaa tcttttagcg cagctatggt
    16381 aagcttatct aaggaaattc aggataaaat tttagaagaa gcaacagcaa aaaatcaaga
    16441 tcaattagta ttaatgaacc gtggtcagcg tgtattaact acacaagaaa caaaattcta
    16501 taacgaagta gtgaaaaacg aaggttttgc aggggtcgaa gaattagtgc cagctactgt
    16561 atttgaacgt gtatttgaag atttagaaca atctcatcca ctattgcaaa aaattacttt
    16621 tgttaacaca actggtgtaa cagaatggat tgtgtcacgt ggagtcaatc cagcatggtg
    16681 gggtaaactt tgcgaagctg ttaaaaaagt tttagataat ggctttgatg taattaacat
    16741 gaagcagttc aagctatcag gttatattcc tgtatgtaag gcaatgcttg atttaggtcc
    16801 agtatggtta gatcgttatg tccgtactgt tttagtagaa tcattgagaa ttgcattaga
    16861 acaagcaatt gttgatggta ctggtaaaga tatgccagtc ggaatgatgc gtgacatgag
    16921 caaacaaact agcggagaat atgctgaaaa aaaagcagaa cctattacag ctttagatgc
    16981 tgtaactatg ggcggtttga tggcgcgact atcaaaattc aatatcgaag gtgtgaatga
    17041 tccgatttat cgtaatgtga atccttctga tgtggtccta attgtgaatc caactgatta
    17101 ctggtctaaa gtattcccag ctaagactgt actaactgct aatggagaat acgtacaagt
    17161 attgccagta ccagtttcag atttgcagtc aacggctgtg ccagaaggaa aagcagttat
    17221 tggggtagcc tcagattact tcatgggtgt aggatctaca ctaaaaattg aagcttcaga
    17281 tgaataccat tttgttgaag acgaacgcat ttatctagct aaacaatatg caaacggtca
    17341 acctaaacgt aacgatagtt tcattgtatt agatattagc gctttgggaa ctactactac
    17401 aactacaaaa ccaacaacca caacaactac aacacaagcg taggtgatca gaatgaagta
    17461 tattctttgt cagccggcaa tcaatcggtt taaatgggag cttgaagttt gtttaactaa
    17521 tctgaagaaa ctaggaatca aagatatcgt attgcttttc agcagacacg atgatcagat
    17581 tcctattttt tttgagaagg aatatggtgt tgaagttcat gtgtacgatg atctgcggga
    17641 cgacaaagag tatattcctt cgattaaacc atatttatgg tggaaatatt tagaagaaga
    17701 tcattcgcgt gaggacgacc gatatttcta tatcgattcg gatgtcattt tcaataaaag
    17761 aattaatttg cgcaaattgc cttctaaaga tgatgtttgg tattgtagcg actgctgtag
    17821 ttatctaagt cttgattata ttagaagctg tgaaaacgga gaaaatattc taaaagatat
    17881 ggcaaacatt gtaaatgtta cagtagaatc tttggaaact ataaacacta attcaggagg
    17941 cgcacagtgg gttattaacc gtcctaaagc gaattattgg aaaaaggttt atctggattc
    18001 taatcggcta tatcgctacc ttagagggca aaaaacaaat atacaaatct ggacagccga
    18061 gatgtgggca cagctttgga acatgatgta tttcaatatt ggtcctaaag ttcacgagga
    18121 attagacttt tgttttgcta ctgatccaat agaaaaagtt aaagaagtaa aaatcttaca
    18181 caatgctgga gtaacaacaa acgatgaaga tttatttttc aaagggagat acgtgacttc
    18241 tacgcctttt gatgaagatt tatcatttgt aaacaagaaa aaatgctctt acgcatatgt
    18301 taaagcaatt aaggcggtgg ttagatgacg cctgaacaag tgactgaaga attgctaata
    18361 gctgtgaagg ataatattta cgttacctgg aacgaagaag atgagtcaat taaaaagatg
    18421 atagctaaaa atgctgttta tcttcaaagt aaagtgagta caacactttc tttttctcct
    18481 gaaagcttag aatacggatt gctaatcgaa agatgtagat acgactggaa tcgtgcttta
    18541 gatgagtttg aacaaaattt cgctagtgag ttattaggtt tcattcaaca ttatgcgcta
    18601 caagaatata ttgcaggtga tgtgaatggc gaataatcgt agactcgaag aaacattcaa
    18661 cgatggttgg ttaaagattt tgacgcaaac taccaaaaga aatgaactag gaaaaaagat
    18721 tggtgtagaa gatacagaaa tcacttcttt aaaatttaga aatctttcca tgagagatag
    18781 tgatataaca gctatggatg cgatgggatc gaaattaact aagaaagtaa agactccatt
    18841 tcatccaatc gccaagaaat ttaataaaga tcaatatttt atcgtaatcg atagtatgcg
    18901 ttacaacgtt atctatgccg attacgataa tttttatatc tatttttatc ttgaaagtgt
    18961 gggtgaatat ggtgattgat aattctaaag aaaaagaacg tttaaataag caaatttctg
    19021 ctatcaaaac ttccttagaa gagcattttg gcctcaaact ctttcaagac tccgtaggcg
    19081 aggatgagct acctgatgat tttaattact tcattctcga aacaggagaa atagaaatga
    19141 tcactgagcc aaaatatagc gtgggtcaaa atctatatct aactttctat tcagaaaata
    19201 gagaagattt aacaggagac tcactagata ttatttcatt gattcaaaat cgttcgattc
    19261 gttttcagag aatggatccc aatcatttaa aactagagaa ccaagatcgc tatatcgatc
    19321 aattggtatt tacgtttaga cgattattga agagtgattg tcatggctaa aaatagttgg
    19381 gagctaaaaa taaatggaca tgatgaactt cttgtgcgga tggaacgcta ttcaagcgag
    19441 agcgaacgac tgattaacga agcattgaaa tcaaaaggtt cggctattgc agtggatagg
    19501 attacagaaa aaattcctgt ttctgaagca gatttaagaa gagggcacca acacgcaaaa
    19561 aatagtcgtc cacttaagac tcaatacatt aatttgggtt tcatcattag acctacaaga
    19621 aaatttgagt atttaaaata tcctgatttg gggataggta cttctaaaag aaatcagccg
    19681 gacgaattta tgagaagagg attaggtctt gcacttgatc caattacaga acttctgatt
    19741 cgtcaattcg ataaattaaa taaatagggg gaacaacaat ggctaaaaca aaaactgtag
    19801 taacaacgtt cgataacgta agtatcaaac gaattgcttt taattttaag aacgcaggaa
    19861 atgcaatcgc aacgaattgt aatggacaat tagatggcga aacagaaatg caaacggtgg
    19921 ttaaaaaatg tggagcgaca gaagtaaaat caaaatctaa accaatcaat atgacggtaa
    19981 caattactgc acatgtaccg atggaagttt atcgacgttt caatgggttg aaacaagatg
    20041 gacgtattaa accaggcatt tactcttacg gtcctgattc cgtaggcgaa gatttctcac
    20101 ttgctgcaga gatcgtggat gacttcgaag aaaatagaaa gttagttggt atgttagcat
    20161 gcacttcgaa tacaggatta acattctcta ttgaaaatgg tgcggatgaa gtagctgcgt
    20221 tagaactaga aacaaaagtt atgcaagatg aatttggtaa attttatcat gaagcaattg
    20281 ttgcagaact tgaagaagac ttaacagatc aatggatgac gaatctatct gctgatgtga
    20341 ttaaaaaaac ttcagttgtg acaactacgg ccactcaatc acagtaaaaa aaacggaggt
    20401 agcgaaatga acgaagatta ctcaaaaatt gaactaaacg atggaacaat tttgaattta
    20461 gaacctaaac tgaatatcaa gaaattattg atgatcaata gagattttaa cacagacgag
    20521 tttgcaaaaa tgactgtggg aaaaggatcc atggatattt ctgttattca aggtgcaaag
    20581 gctgtgtata ttgcttaccg ccaagcgaac atgactgatt atatttcatt cgatgaattt
    20641 atcgataaat gggattttga tatggctact gccagctata tttatcaatt gatgatgttc
    20701 aaacaagcac gcgatgctta tcaaaaagaa tttgaaaaag caaataagga aaaaaagctt
    20761 caaaagtaaa aattccaaag ctcttagttg aaacgtgggt cgatgtctat tcgatgttga
    20821 ccgacgtttt ttctatgcct tcagatttgg ttttaagcga tatctgttta gatgacattt
    20881 tgcaaatggc ttacaacaag agtgcttatg aaggatggaa aaactatgca ataaaccaat
    20941 cccagaaaaa ctaaagaaag gaggtaaaaa atggctaaaa agagaacaga agcagaagta
    21001 actttcatag ctaacgatga cggattgaaa tctacgttaa aagaaatcag tgctgaatta
    21061 actaaaaata gagcagaatt aaaactagaa caagctcaat tacaacagac tggttctgaa
    21121 tcagacaagt taggaagtaa attatcttct ttagaaaagc agtatgaatt acaaagtcaa
    21181 aaagttgaag taactagcca acgtttagcc aatgcgaaaa aatattatgg agaaaattcc
    21241 accgaagttc agaaacttga aagagaactg attaatcaac aaacagcgca acaacgtttg
    21301 tcaaacgaaa ttgataaaac gagtaatgca ctagctcaag caaaaggcga aatacagacg
    21361 tacgagtcta caatgcaaca gttggatagc gaacaaaaaa atgttcaagc tagtgcttct
    21421 ctgattgaat ccgaatacaa aaaatggcaa gcaactgctg gtcaatcagc ttctgaagcc
    21481 gagaaattag cgaaagccca agaatatgtt tctcaacaat ctgaaaatgc ggagaaaacg
    21541 atagatatcc taagacgaca gttagaagct acacagtctg aatttggcgc tacatccaca
    21601 gaagcaatgc agatggaagc gaagcttaat gatgctgaac gtgaatttga agagttagga
    21661 caagctgcta aaaatgtaga tacaactaac ttggacgata tcggaagcaa aatagatatg
    21721 aataatttaa tggaagcttc tgacgtttta agcgacattg gcgataagct tacagaatta
    21781 gggaaacaag cagtggactc tgctaacagt gtaggtagtt cccagagtaa gatacaagct
    21841 aattttggtt tgtctaaaca agaggctgaa gaattaacga atgtagccag agacatttat
    21901 tataaaggtt ttggagaatc gttagatcag tccacagatg cattgatttt ggtaaagcgt
    21961 aatttaggag atttaaataa tcaagattta caaaatatca cggaacaagc tatggtccta
    22021 gaaaacacca tgggcgctga tatggatgaa acgttacgtg gtgtaaatgg cttaatggtc
    22081 aatttcggct tgagtgctca agatgcaatg gatttaatgg tttcgggtac tcaaaacggt
    22141 ttagataaaa cgcacgaatt aggcgacaat atggcagaat atagccaatt atggagtcaa
    22201 atgggatatt cagctgatga aacgttcgga atgcttcaaa atggtttaga tgcgggtgct
    22261 tataaccttg ataaagtcaa tgacttagtt aaggaaatgg gaatatcgtt aacagatggt
    22321 cgatttgagc aaaacatgga tatgtttagt gaaagtacta gaaaagcttt tgaagagtgg
    22381 aaaaatggcg gaggaacaca aaaagacgtt attaattcca tgattcaaga ttttagcaat
    22441 atggatggtc aatacgacca attaaataaa gcttcgacaa tttggtctgc gcttggcgaa
    22501 gataatgcga tgaaagttgt ccaatcttta actgatgtta accatacatt tgatgatgtt
    22561 agtggatctg cacaaaaaat gaatgaagat tctactactc cgttgcaaga gttgaacggg
    22621 aaaatagctg aattaaagga ttcattagct cctataggca acacaatcat agatgcactc
    22681 gaaccagtaa ttgattttct aggaaagatg gctgatgcgt ttaataatct tccacaacca
    22741 gtacaggatt tcattgttgc tataggtggt cttacagcag catttggcat actgttaccg
    22801 gcaatactag ccgtgtcata tttatttggt ccgatgatgc ttataattgg aggaattata
    22861 gctgttatag caggcgttat tgtggcaatt aagaactggg gtgcaattac tgactggttt
    22921 agtggtttat ggaaaaaatt tactgattgg ttgggtgata cttgggaaag tataaaagac
    22981 ggagcctcat cagtttggga tggggttaaa gaaacctggt ctggatttgt agattgggtt
    23041 caagatattt ggcaaggagt ttctgattgg tttggagagc tatggagcgg attagttgaa
    23101 ggagcttcca acatctggca aggagtccaa gaaacttggc aaacattcgt tgattgggtt
    23161 tcaaatattt ggaacggagt caaagaagta tggtcgatta tttgggcaga cattgtagga
    23221 attgttcaaa taccatggac attaataacg tcattgattc aagctggtat taatattatc
    23281 gtgggtattt ttgatgtagc tggacagtta ttgggcgcag cttggcaagc tgtttggaca
    23341 cctatttctg atttccttaa aaatacttgg gatactatga cacaatggat aagtatcgct
    23401 tggaatggga ttgtaactac attccatact atatttgatc cagtagtggc atggtggaat
    23461 ggtatatgga cagctattag tactacggct tcaaatattt ggaattcaat tagtgcaaca
    23521 gcttctagta tttggaacag tatcaagaat acaatcacta gcttggtaca agcagctgct
    23581 acagtaattc aaaatatttg gtcaactgta tctagttggt taggtggaat ttggaattca
    23641 atcagctcta cagcatcaaa tatctggaat agtgtgacta gtagtataag caatgctata
    23701 aacgcagcta aaagtgccat tcaaagtgtt tggaatagta tatcttcgtg gattagcgga
    23761 atttggaacg gtatcaaaaa tactgctttg aatctttgga atggaattac aagcactatt
    23821 agctctaaag taaacgatgg aaaaaatgca atttcaagcg gttggtccaa tctaacaggt
    23881 attgtttccg acatattcaa taatgttaaa agtacaattg ctaacatatg ggaaggcatc
    23941 aaaaagactg ttagcgctcc aattgattgg attagagaca aaatcagtgg catttttgat
    24001 aatttgaata tttctatacc acatattccg ttaccggaat ttatcatgga aggcagcttt
    24061 aacccgctaa aaggtcaaat cccccatttg cgtgtaaaat ggcatgctaa aggaggtatc
    24121 tttactaaac caactttact aggtggaatg aacggtgtcg gtgaagcagg acctgaagca
    24181 gttttacctt tgaaaagatc tgttttgcaa gaaattggtg atcgtatctt gagtagcaca
    24241 tcagtttcat ctagggcaca aacgattcaa cctgtgaata actacgaatt caatttcaca
    24301 attgatggta acgcagatga ggttactatg aagcaaacaa ctcaacaaat cattgatagc
    24361 attacaaaaa ttcaaaatga taatgcttcg gcatggcgtt aaacaggaga gtatttctcc
    24421 tgttttttta gtattaaaaa ggatgtgaaa aaatgactga ttgtatacat tctataatcg
    24481 atggatttcc tgattatttg cataaattgg ctttagctga aagaccaacc ataccttctc
    24541 caaaaagaca gagagttgaa acttctgttt taggtaggtt aggtggctta gtacaagatt
    24601 actcgtttga agatatgtcg tttacattgc actataacta tttagaggat gtggaagacc
    24661 atcaagcgtt caagcaatcg ttttatatca tgcgtcattg gttaaactat gcaaagaaat
    24721 tagaattctc tgacgatccc aacgtctatt acgttatcca gactatcgat attggggatg
    24781 cagaaaacga tattgttgaa tggggagagt tcgatgtaaa tattactgcg aaaccattcg
    24841 caagagttca agaagatgta cctataaccg tagataaacc acagtcattt aacttgctga
    24901 ataatagttt agaagaaagt tttccaaaga ttatcatcac tccttcagct acttcatgcc
    24961 agttcatctt aaatgattat gtgtttagtt tcgaaggctt agtagcagga actgatgtag
    25021 tcattgatag tgatttgatg ctttgctacg aagagcaatc ggacggagat attttagatc
    25081 ggtccaacaa aatgaagacc atgcaatatc cgacattgca agtggatatc aatcatttta
    25141 attgcactgg tttgagcaaa atacaaattt atcgtaacgg gttaaggtag gtgaaataga
    25201 tgatcgataa tttaataact atttacgata aaaatgacgc gaataattta gctgaacatt
    25261 tatatgatac gcaaggttta ggcgctttgt cagactggtt aacagctact gttagcaata
    25321 aactaaacgg agccgagata tttcagggta cttattcaat aaacggaact aatgcagatt
    25381 tgattgtaga cggacgtatt attcagtgtt atgtagatga aaatcgagca aaacagcgtc
    25441 tacggattta ttatgcaaaa acttctgtaa taggcaatac gatagaagta aaagctgaac
    25501 ctattttcaa tgatataaga aaatcggtgt tgaataaata tgacagcgga acagaaaaga
    25561 tcactgctac tcaggcatgg caaaacgcaa aagttttagc gaaaccagct atcccttcgc
    25621 agttttcttt ctcgtcatta gtagatacgc ttgctaatgt gaagatagaa aaggcgaatt
    25681 ttttagaatt ctttggtgga aaagagggat ctattctaga tcgatttcat ggtgagtttc
    25741 taaaagataa taacacatta cgtcatgaaa aaagtctagg tacggatcat aaaatcaaag
    25801 cgatttatac taaaaactta actggtcttg acttagagat tgatgctcaa agtgttttag
    25861 ttggagttta tccattcatt agcagctctt cagaaggaga agacgagatc actctaccag
    25921 aagaagttat tttcacggat tacgtggatg attatcctgc tggatatgtt tcttttgttg
    25981 attttaaaga caaagcgact gatgtagcca cattaaggga agctgctaaa gactggttga
    26041 aaacaaacat agataaacaa aaaccacaag tgagtggttc gattgaatta gtaccattga
    26101 ggcatcaaag aggctatgaa aaatttgttg atctagaaaa agtttcgatg ggtgacggag
    26161 tagatgtgta tcatccacag ttaaaagtga atatgtcagc gagaattgtg gaatatacgt
    26221 ttaatgttct aaccaattca tacgataaat tagttgtagg aaacgtcaaa acaaacttct
    26281 tagaaaacac agagaataat gtcagcaatt tgattaatga tgccattgat caattgaaaa
    26341 acggtggcga aatcagtgat ttactcaatg atattgtaga tcatcaaact gatatgatta
    26401 ctggtcaaaa tggtggttat gttttattag atcctaaaga agcgcctagt cgtattttga
    26461 ttatggacac accagataag aataccgcaa ggaatgtttt acaaatcaac aatgctggta
    26521 ttggtttttc taaaactggc attaatggaa catatgaaac ggcatggacg ttagatggcg
    26581 gattcaatgc ctcgtttatt acggctggtg aaatagtagg aattactatt agaggtacta
    26641 cattaattag tgatggtgct gattatagaa caagtattgc taatggcaaa atgacttggt
    26701 actcaaaaaa agttaacaaa gatattatgg agctagaagc acgtgattat gtaagtgctg
    26761 atgccggtat tgtatcatac accatgaaaa ctggtggtgg tttcatgatt agaaatccac
    26821 agggtaactt ggtttttagt acgtgggata atggtaataa cagaccgttt ctatcttttg
    26881 gtgcgcccaa tttcaggtat agcaatgcta gttatgtaac ttctggcgac ggtagttctt
    26941 taagcattaa tggtagtgcg ggtaactcat gggaatttaa ggtagctggt aggactatga
    27001 aatttactag tgatggtatg ctaacgttac caggttgttt ttttggttca tgggaagatg
    27061 ggaaacttgc taggtttgaa caatcaacgg tacaagtata taaagatttt actgttagag
    27121 gtactaaaaa ctcaactgta ccaacagaac attatggaca acgactattg aacgcttatg
    27181 aaactccaga atattatttc gctgattatg gggaagccgt tacaggtgac aatggtaaag
    27241 ttcgtgttga tattgacccc atgtttgctg aaacagtaaa tctaagtcgg tatatgacac
    27301 atgtgacacc tacagaacta gttttgtgtg ctgttactca tgaagatatt gaccatttca
    27361 tcattgaaac tagtaagcca aacgtattag ttagatggaa tttagtggca caccgtctag
    27421 ggtatgaaga tattagatta aaagaggata cagcatatga tagcacagtg cttgaccaaa
    27481 aacgttttta aaacgaagac aaggaggtat ataaatggct agcagtttat ataatttggc
    27541 tttagatttc agcaaagaat taaactacac caaagctatt atggctcgtc aaggtgataa
    27601 agggattacg gtgacggtta aaccgtttct aaatggcttg cagatggata cgagtggcgg
    27661 aacatttact ttaaaaggaa caacaccatc taaccgttac gtagataatg ttgcaactag
    27721 tgtaactagt gaagaagtca cgttttctct tgatggcaca tttatgagtg aagcaggata
    27781 ttataaacac tgctacgtag aatatagaaa agacaatcaa attttaacaa cgcaagatat
    27841 catttttttc tcactaggag tgtctgacat ttcgcaaggc caagccgatg aatatgtttc
    27901 gcaattagaa gagttgattc gaaagtacaa cgaaactttt gatgctttta tggctgaaat
    27961 caaaggtaga gtggatagct taaatcaaca gattactgat ttaactggtc aagctaaaac
    28021 gctacaagac aagttagatg ctctgaaaga agaaatttct aagttaggta acttacaagt
    28081 gatgtacagt aacagcatcg acttcgggga ctatgattat tcggagaacc cgaatttaat
    28141 gccatatatt acagaacctt gggttggacc tttgttaggt aatggacata cagttaaaga
    28201 tagtgttaaa agggttataa ctcatactaa aactagaaca gctaattccg gcgacatatt
    28261 atcgcttggc ttaggtattc cgtgtacagc ggaagctaat aataggtatc ttataaccac
    28321 tttacgacca tctacgacat acacattaag cgttactatg agtgtcggtt cagattggac
    28381 tggagagacc aatactatag gggttaggct tagatattta aatgaacaag gtggtataga
    28441 actgcccatt aatgcattaa taccagcaaa tgtagaacgc gataaaatgg tcactcatac
    28501 atttactggt ataacaaaag ataatgtaac tagcataaca aactgttatg ttgaaatatt
    28561 ctcacttaat agtgaatata aaggaacggt tagtgtcagc tatgacgtta agttaaaagc
    28621 acactaccct aacttactag atggccctta ctggttaggt aaagttcctt taggcgagaa
    28681 tattgctgac cctacagttg tctttccaca caaaactagt gaatatatgg tttatggtag
    28741 acgcaataca gaaaactata tcgctgacca aacatatacc atatcaatga aagcaactaa
    28801 actaacagtt caatcgtttg ccgtgtatat tgctgctggc cgcgttaaag taggagacat
    28861 gaagcctact gaggggttag ctaatacatg ggagttaact tttactgtaa ctaagcaaca
    28921 tatagatagt ggggtaacta attatctgga aatataccaa tacccgtcag ctacaaaagg
    28981 agctgttcaa attgagtggc tcaaactaga aaaaggcaat actcgaaccc cgaatattag
    29041 tgagtataaa tatcgtggta ctggtatgcg tgattcaaac aatccaaaag attatgtttg
    29101 ggatctagca ccagaatatg tcgaagataa tttggccaca gatattaaaa tttctgaaat
    29161 tactggtaaa gcaaacaatt ataccgatgg gaaagtatcg gagattaatt cgcagttgac
    29221 tgcttcaatt aatgaagtag acaccacagc taaggatgct caaacaaaag cgaatgctaa
    29281 tgcgactgct atagatgaat tagacaataa gatcgatgaa cgcattaatg atacagctac
    29341 taccacatta acagttacaa acgggaatac cggatcagca aagctttatc gtgaaggaaa
    29401 aacagtttct atatattttg tggctttaaa cggaaaaagc agtggtggaa atgattcaac
    29461 gatactaaca attccagaag gctatcggcc accaattagt tttgagcaac tggttggctc
    29521 gatagaccgt tctactttga acagtgctca gttatctatt ggtgcagatg gagccattaa
    29581 atggcgaaga aactcaagtt atggatcgga ttataccttt gcaattactt acacgattta
    29641 gaaaagcgtg aatcgatatg aaggcagcat atagaccaat tgaaccttac ggattcgagc
    29701 aaatcattgt gaatgatgaa gaacatttac cggaagaatg cacagaagtc gaaccaccga
    29761 ttccaaattg gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa acatttggaa
    29821 atatggacgt actggcggag agtacgcagg aaaagtattg gacgacatgc ttgtatccgt
    29881 tccttacaca gatcagccac cgcttgaagg aattcgtgct gatggcgaac cgttaacaat
    29941 cgctgatcaa atgtttgatc ctaaattgaa tcaatggatt attttagcga acgcactaga
    30001 tcacaacgat ttaaacaatc tcaaagcaat gtatgagtcg ttagaaaatg agaacggcga
    30061 tttaaaacag atcaatgcca aactcatgct aagcgatgta gcaattaaac aggaaaatac
    30121 tgcattgaaa gaaaaagcgg atagtttagc acaaatcaat tcaaaaatga tgcttgcttc
    30181 gttacaaaat agcaaagaca tttcagaaat taaagagcaa ctaaatccag cttcaaaggg
    30241 aggtgagtag tatgtttagt tttagcgatg tgaaaatgat gtatgattgg ggctgtttca
    30301 ctaacgaaca agtaatggtt ttcgttccgt tgtgcattac tgaagaaaaa gcagataaaa
    30361 tcattagcaa agaagagagc gcatcttaat tgatgtgctt tttattttga ttcaaggagt
    30421 tgtcacatga ttaatttagg ggaatgggga gcgatagcag gatcaataac cgctatcgtt
    30481 tctttgattt tattagtaat aaaaccaatt actgcatctt tctcgaagat tactgagact
    30541 ctttcaaaag taagtcacaa tttagatttg ctgactaaag atttagaatc gagcaaatca
    30601 gatcgattga tgattcatga agaactaaag aaacacgatg aaagattaga tacacatgca
    30661 gaaaaattgg tagaacacac gcaacaaatt aaaactttat ttagggagag aagaaaatga
    30721 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
    30781 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
    30841 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
    30901 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
    30961 tgcgatgagc ctgttaacgg ctcttttttt attacccatt aatacgtttg cttatactat
    31021 taatgacgag tataatttag cgccgaatca aggagactcc agattagcaa ttcctaacaa
    31081 aattattttg cacgaaactg gaatagatgc accagcaaga aacgtagccg ccaacatgaa
    31141 aaataattat aacggaagca attcttatac tacagatgtt attggtgacg gtgggattgt
    31201 ttaccgtgtg ggtgagcaag gatatgtttc gtggggagct ggtaacgcta atccttatgc
    31261 gcctgtacag attgaattac agcgcacata tgataaagca ttgtttgaaa aaaactatcg
    31321 agcttatatt gaatatacaa gagatagtgc aaaaaaatat ggaattccat tgactcttga
    31381 tcaaggaact tctttattta caaaaggaat catttctcat ttgtgggtga caaattatgt
    31441 ttgggggaac cacacagatc catatggtta cttatcgcaa atgggagtta gcaaagaaaa
    31501 gcttgcttat gatttagctc atggatttac cgatgaaaat ccaacaactt ctgaaaacaa
    31561 gcctgtcatt gatccaacac gagctggtgc agctaatcct acgctgacag atggaacaaa
    31621 tcacgcccac attgatcagt ttggggaaat cgaaaacgca aacttgcatg tggctggatg
    31681 gcacattgct aactataaat acgagtatat tttcattatg gactacaata ctgggaaaga
    31741 attagctcga gtaagagctg atggaattta tagaccagat gtaaaccaag cttataatac
    31801 ttctggaaat gttggttatc atgtatcttt caatatgcgt aattttccta gtaagaaagt
    31861 ctatgtaatg atgcgggcaa cgaatgatcc agagggaaac actaaaggcg gtgcgcaaga
    31921 tttccatgac aaacgttggt atttaaatat tcctaaacga taaaaatagc tcctcgttga
    31981 ggagcagtac ataactatat tgacaactat aaaaattatt cgataaaata gtgatgttat
    32041 cgcatatctt cactatcacc cataaatagt cacactccaa gctatgcgat aacaggtttg
    32101 ttgccacaca ttctactggt tgattgttta tggctttatg tggcaacaac cagtaccctt
    32161 agctcagt
//
LOCUS       DAZKCZ010000024        31784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000024 DAZKCZ010000000
VERSION     DAZKCZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.024"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..159
                     /locus_tag="ACG8KN_000977"
                     /pseudo
     CDS             112..159
                     /locus_tag="ACG8KN_000977"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            175..975
                     /locus_tag="ACG8KN_000978"
                     /pseudo
     CDS             175..975
                     /locus_tag="ACG8KN_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            1213..1449
                     /locus_tag="ACG8KN_000979"
     CDS             1213..1449
                     /locus_tag="ACG8KN_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188054.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1456..3408
                     /locus_tag="ACG8KN_000980"
     CDS             1456..3408
                     /locus_tag="ACG8KN_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9188055.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3480..3977)
                     /gene="dfrG"
                     /locus_tag="ACG8KN_000981"
     CDS             complement(3480..3977)
                     /gene="dfrG"
                     /locus_tag="ACG8KN_000981"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9188056.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            4252..5379
                     /locus_tag="ACG8KN_000982"
                     /pseudo
     CDS             4252..5379
                     /locus_tag="ACG8KN_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            5477..5665
                     /locus_tag="ACG8KN_000983"
     CDS             5477..5665
                     /locus_tag="ACG8KN_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP9188057.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(5723..6076)
                     /locus_tag="ACG8KN_000984"
     CDS             complement(5723..6076)
                     /locus_tag="ACG8KN_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9188058.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            6607..7029
                     /locus_tag="ACG8KN_000985"
     CDS             6607..7029
                     /locus_tag="ACG8KN_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9188059.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            7026..7256
                     /locus_tag="ACG8KN_000986"
     CDS             7026..7256
                     /locus_tag="ACG8KN_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9188060.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            7754..7954
                     /locus_tag="ACG8KN_000987"
     CDS             7754..7954
                     /locus_tag="ACG8KN_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFP9188061.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7981..9174
                     /locus_tag="ACG8KN_000988"
     CDS             7981..9174
                     /locus_tag="ACG8KN_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9188062.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(9242..10807)
                     /gene="guaA"
                     /locus_tag="ACG8KN_000989"
     CDS             complement(9242..10807)
                     /gene="guaA"
                     /locus_tag="ACG8KN_000989"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9188063.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10866..11789)
                     /gene="coaA"
                     /locus_tag="ACG8KN_000990"
     CDS             complement(10866..11789)
                     /gene="coaA"
                     /locus_tag="ACG8KN_000990"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9188064.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11985..12590
                     /locus_tag="ACG8KN_000991"
     CDS             11985..12590
                     /locus_tag="ACG8KN_000991"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9188065.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12898..13914
                     /locus_tag="ACG8KN_000992"
     CDS             12898..13914
                     /locus_tag="ACG8KN_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9188066.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            14055..15356
                     /locus_tag="ACG8KN_000993"
     CDS             14055..15356
                     /locus_tag="ACG8KN_000993"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFP9188067.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            15402..16064
                     /gene="deoC"
                     /locus_tag="ACG8KN_000994"
     CDS             15402..16064
                     /gene="deoC"
                     /locus_tag="ACG8KN_000994"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9188068.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            16085..16483
                     /locus_tag="ACG8KN_000995"
     CDS             16085..16483
                     /locus_tag="ACG8KN_000995"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9188069.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            16549..17631
                     /locus_tag="ACG8KN_000996"
     CDS             16549..17631
                     /locus_tag="ACG8KN_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9188070.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17843..19405
                     /locus_tag="ACG8KN_000997"
     CDS             17843..19405
                     /locus_tag="ACG8KN_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188071.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            19398..20531
                     /locus_tag="ACG8KN_000998"
     CDS             19398..20531
                     /locus_tag="ACG8KN_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188072.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            20531..21487
                     /locus_tag="ACG8KN_000999"
     CDS             20531..21487
                     /locus_tag="ACG8KN_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188073.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            21586..22752
                     /gene="deoB"
                     /locus_tag="ACG8KN_001000"
     CDS             21586..22752
                     /gene="deoB"
                     /locus_tag="ACG8KN_001000"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9188074.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22776..23594
                     /locus_tag="ACG8KN_001001"
     CDS             22776..23594
                     /locus_tag="ACG8KN_001001"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9188075.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            23611..24318
                     /gene="deoD"
                     /locus_tag="ACG8KN_001002"
     CDS             23611..24318
                     /gene="deoD"
                     /locus_tag="ACG8KN_001002"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9188076.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(24616..25302)
                     /gene="gpmA"
                     /locus_tag="ACG8KN_001003"
     CDS             complement(24616..25302)
                     /gene="gpmA"
                     /locus_tag="ACG8KN_001003"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9188077.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(25474..26055)
                     /locus_tag="ACG8KN_001004"
     CDS             complement(25474..26055)
                     /locus_tag="ACG8KN_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9188078.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(26188..26871)
                     /gene="rpiA"
                     /locus_tag="ACG8KN_001005"
     CDS             complement(26188..26871)
                     /gene="rpiA"
                     /locus_tag="ACG8KN_001005"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9188079.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            27190..27603
                     /gene="rpsL"
                     /locus_tag="ACG8KN_001006"
     CDS             27190..27603
                     /gene="rpsL"
                     /locus_tag="ACG8KN_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9188080.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            27687..28157
                     /gene="rpsG"
                     /locus_tag="ACG8KN_001007"
     CDS             27687..28157
                     /gene="rpsG"
                     /locus_tag="ACG8KN_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9188081.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            28251..30338
                     /gene="fusA"
                     /locus_tag="ACG8KN_001008"
     CDS             28251..30338
                     /gene="fusA"
                     /locus_tag="ACG8KN_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9188082.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            30510..31697
                     /gene="tuf"
                     /locus_tag="ACG8KN_001009"
     CDS             30510..31697
                     /gene="tuf"
                     /locus_tag="ACG8KN_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9188083.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT        10177 a         5743 c         6592 g         9272 t
ORIGIN      
        1 gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct aaagtatcca
       61 gagaatatct gtatgctttg tatacctatg gttatgcata aaaatcccag tgataaaagt
      121 atttatcact gggattttta tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa
      181 attattaata ttggagtttt agctcatgtt gatgcaggaa aaactacctt aacagaaagc
      241 ttattatata acagtggagc gattacagaa ttaggaagcg tggacaaagg tacaacgagg
      301 acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacagg aataacctct
      361 tttcagtggg aaaatacgaa ggtgaacatc atagacacgc caggacatat ggatttctta
      421 gcagaagtat atcgttcatt atcagtttta gatggggcaa ttctactgat ttctgcaaaa
      481 gatggcgtac aagcacaaac tcgtatatta tttcatgcac ttaggaaaat ggggattccc
      541 acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac ggtttatcag
      601 gatattaaag agaaactttc tgccgaaatt gtaatcaaac agaaggtaga actgtatcct
      661 aatatgtgtg tgacgaactt taccgaatct gaacaatggg atacggtaat agagggaaac
      721 gatgaccttt tagagaaata tatgtccggt aaatcattag aagcattgga actcgaacaa
      781 gaggaaagca taagatttca gaattgttct ctgttccctc tttatcatgg aagtgcaaaa
      841 agtaatatag ggattgataa ccttatagaa gttattacta ataaatttta ttcatcaaca
      901 catcgaggtc cgtctgaact ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga
      961 caacgtcttg catattaagt agttcagttt tggagtacaa aaagtgaaaa tgtaaaccta
     1021 aatgagtatc aaattgcaac tgaatttgat actcgttttt tattgttaaa tcaacgtttg
     1081 ttgttaattt acaccacaaa atgtaagtta aaatgtatat aaaaatgcga acgtgaaata
     1141 attattgtag gaatgcaaat tggtgtgtta aaatggttac aatttactaa gcaaaaaggg
     1201 gtgcattttc ctatgcaatt tcttgagcca aaaaataaga acgctaagtc tgtggattgg
     1261 gaaatatcag agcaagtaag ggtgatagtt aagcaatacg ctgaatatgc agagcgtact
     1321 gaaagcgaag cagtggatga attcttattg aacattttag acgataagaa gtttattgaa
     1381 tggattgcca acaagcgaag taataagcgg attgttgaaa aaatgggtat caaagataga
     1441 gtgggttgat agacaatgac caagcttaaa agattagcca cgaaggaaga tgaaattgta
     1501 gatgttaaaa ttccgatttc aaacgaagaa ttaaaagata gggctaaaca atatgattta
     1561 ttaactccaa aacgatttgc aacaaggtat aacaaaatgt tgttcttgcc aacgtctttt
     1621 aaatggaacg gttctgaata tcccattcaa tataattact gcatcaatcc gttttgttgt
     1681 aattttggga aagaacagca caaattcaaa gatgtaaaag ggaaaccaag tcgatataaa
     1741 atgactggta gttccaaaga taaaggtcat aaaggtatgt actgcaatga caatcctatt
     1801 ggcagaggtg tatcgcaaaa ctgtacagta acccctttat ctaattggtc tgttgttgaa
     1861 gaaattaaac gccttataga aatcaatagc atccaagatg ttgagccaga ttatcaattt
     1921 cataaagaag gctgctcgga agaagaatca accccattca atgagccgaa gcaattttac
     1981 aaaaggggga aaagtagggg caaatctcaa cgctatcaat gtaaggcgtg taagaagttc
     2041 actaatgttc taccaaaaag agaagaaacc actacttatc accaacaaaa gaacaccatt
     2101 cttccaatgt tcgctaagat ggttgttgga agggtttcag tcagtcgtac ttgtgatatt
     2161 ttaggaattg gagttggaac ctactatcat aagctggagt ggctgtatag gcgttgtttg
     2221 gagtttttag aacgatatga gacacaaccc cttcaaacaa agaaatttaa cgagatgtgg
     2281 cttaatacgg acaagatgca ctactaccta aataacgtcc gtaagaaagg acaaggttca
     2341 aagaaatata cggggtttga agaccttaat atgcaaacgt atatagttgt atctgctgag
     2401 gtcttatcaa gatatgtgtt ccgttctgat gttgcctatg attggaatat ttcaatggat
     2461 gaattaaatg aagatacaag aaaattcaaa gaagaccatc tcaatacttt tagtcgtaaa
     2521 aatgataggt tagattggtc ttattatcca caagaaccat ccgctaacga tagcgaaaat
     2581 aggaatgctt atcttcacga attagggaaa attactaata ggagtagatt tgtcgatggt
     2641 ttaaatgttg atgctcctta tactacaact gctcactatt ggttaattaa acaaatggtt
     2701 aatgccgacg aatggcgaat gattagtgat gatgacttct ctattagaaa cgccttttat
     2761 agagtattca cgaaagaatt aaggttgagc gatgcacatc attttatttg ccaagttaac
     2821 aagacgaaaa gcagaaaaca atgcttaaaa gaattcggac aagcaaaagc agaactgctc
     2881 gattggggag atatcagagg attcaaaaca aagtttctac ggactttggc atctcattat
     2941 ctaactgaat tactaacaag tcatcaattc cacgaagagg ctattagtaa agatggagaa
     3001 agatatcgca aatatgccga taaccctatc aaacatccat tagccactaa ggataaagga
     3061 ttttattcag ttgattgtag aacggattta tctgctctgg aaccaaacga aatcgcaaag
     3121 atgctattaa atgttaatga ccattcgacc aatagcttta ttcaacagat aagacgttat
     3181 atatcatctc ttgaaagacc attaacaaca gctcgtggtg ataagaagag ctatatttac
     3241 gctaacttca atccgaagta tgctcaattt gcgattacaa ttctaagaac ttactacaac
     3301 ttttgtcgcc cttttaaatc agcggataaa aaggttttga cacctgctca aagattaggg
     3361 attacagata aacaatttga ttggaaagat ataatttatt tcaaatgaca aaaagagctt
     3421 ggagtaatcc caagctcttg tgctttatct ttactgttct tcttcaatta acgcacccgt
     3481 taactcaata agttttttct ttcatataca tgaaaatagt agttatacgg atttttatca
     3541 ttctttatcc ctttttgggc aaatacctca ttccattcct cataattcac ttctggaaaa
     3601 aaagtatctc cttcgaattc atgatgtatt tttgtgatgt acattttctc aacataaggg
     3661 aaaaacaaat tataaatctg ttctcctccg aaaataaaaa tttcttcttc gtttttacat
     3721 aactcaaaaa catcttctat tgaatgaaca atttcacaac cattaaaggt aaacccctta
     3781 tctctcgtca gaataatatt tcttctgtca ggtaaggctc ttccgattga ttcaaggttc
     3841 ttcctaccta atattatcgg atgtcccttt gtagtatttt taacatattc ccagtccttg
     3901 ggaatcctcc aaggaatgtc attctctttg ccaatcactc tattcttatc catcgcagca
     3961 atcaaagaaa ctttcattta taacacagcc tttttaacta atttaccatc taatttctta
     4021 tttctatcaa aaaacctttt ctcattatcc tgcccgttag ttgaataagc aattttttct
     4081 ataagttata ttatcataat accacaaaat cacaaatttt cattcaatta tcattttcaa
     4141 ttgcatttta taaatcattt tgtatgtaat ttggtatgta agttaaaatg aaaaaagtag
     4201 tccattttga agtacatttt gtcattttgt actccaaaac tgaactactt acttgcatat
     4261 atacgccttt atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa
     4321 aaaataaaag ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga
     4381 gcttattctg gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt
     4441 ggagatacaa aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa
     4501 acaactgttg aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa
     4561 atctcagata gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata
     4621 ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat
     4681 catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat
     4741 gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta
     4801 tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga
     4861 tacttaaatc aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa
     4921 ggattatatg gttggaatgt gacggattgt aaaatctgtt ttaagtacgg tttatactat
     4981 agccctgtta gtactccagc agattttcgg atgcttactc ctattgtact ggagcaagcc
     5041 tttagaaaag ctggaacaga attgttagag ccatatctta gttttaaagt ttatgcacca
     5101 caggaatatc tttcacgggc atataacgat gctcccaaat attgtgcaaa tatcgtaaat
     5161 actcaactga aaaataatga ggtcattatt attggagaaa ttcctgctcg atgtattcaa
     5221 gattatcgca atgatttaac tttttttaca aatgggctta gtgtttgttt agcagagcta
     5281 aaaggatatc aggttaccac tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg
     5341 atagataaag taagatatat gttcaataaa ataacttagt gcgttttatg ttgttatata
     5401 aatatggttt cttattaaat aagatgaaat attctttaat atagatttga attaaagtgg
     5461 aaaggaggag attgttatta taaactacaa gtggatattg tgtcctattt gtggaaataa
     5521 aacaagacta cgaatacgag tggatactat acttaaaaat ttccctttat actgccccaa
     5581 atgtaagaac gaaactttaa ttaatgttca aaaaatgaat ataataacaa tcaaagagcc
     5641 agacgccaag acgcagagcc gataatttga gaaatgaaac tctcatctta tcggctcttt
     5701 ttgtttatct gaattttact gactagcctt caatatttct tgcttcattg ataccttttg
     5761 ctagtgcttc cattaaggat agttctttgt ctgtaaagct atccatgtat ttttctacct
     5821 gtaaccttcg agtgctttta atcaaattat ctgtaggtaa gaaaaattca tcaatagata
     5881 catggagtaa tgatacaaga tcatacagca cttgtgtact tgggtgttgt cctttgtttt
     5941 ctatatttgt taaataacgt gggtcaattt caatcattgt tcccacttgt tcacgagtga
     6001 gaccccgttt cattcgagcc tctttaatcg ctaaaccaac ggctctaaaa tcgtatttat
     6061 cttctttttt acgcatacta aatcacctct atacatttta ctgttcctat ataaccataa
     6121 acaggtatag aaaaacatgt tatatagttt atgagttcct atagaacact gtggaattta
     6181 ttaatgcgat gaatccaatt tcaaatagaa aggtcgctga ttttatttac ctatacaact
     6241 ttataaaatt tagtcatact cctagaaagt agataaaatt ggcgacttaa tggagggaaa
     6301 agtcgccatg agaaagataa tctgtcatct agttctctta attaagttct tatcaaaaaa
     6361 acttatccac ctgtactgga tagatatgac catcaatgtg gttttgcaga ttacatagat
     6421 agaaaaatca aagcatatga acagagaaaa caatctgttt atgtgctttt ttggtcactt
     6481 aataaatatc ttttaggatt tctcttcatt tatgcaacaa atcccccgtt cgtgtcgggg
     6541 tttagtatga agggagataa agaagcacat gccactccct ttcacgacag aaagggggtg
     6601 aaaagcatga aaccatcttc ttttcaagaa accatagaaa atcagtttga ctatatctgc
     6661 aaacaagtta ttgaagatga acgaaaagat tacttcaaac atctagacag actatcagca
     6721 aaagaagtct cgttttctga tatagataac taccttgtca actctttttc tactgttgat
     6781 acgtacgtca ctgatttcca actatttaaa ttatttggat taagaatcaa tgttgaaagt
     6841 gatttattaa gtgaagccct gcgtcatctt tcagagaaaa aacggaacat tattttactt
     6901 cattacttta tggatatgag tgatgtggag attgcggagt tactaaattt gaatcgttcg
     6961 actgtttatc gtcatcgaat aagtggacta gccatgatta aagaattcat gaaggagtgt
     7021 aaagaatgaa aacagcttat ccaagggttc ccttccccct cattgtaaag gcgacagatg
     7081 gcgacgtgga agcaattaat cagattgtta aacattatcg agggtatacc tcaaaacgtt
     7141 cattacgtcg catgacagac gaatatggaa attctcatat ggttattgat gaaaccctac
     7201 gtggcagaat ggaaacgaaa ctcatctcta agattttatc gttcgaaatt aaatagtccc
     7261 attttttcgt ggaagcgtgt cgaatcgtat ccacgcttcc cgatttggga ggtttgttat
     7321 tccatcaaag catattgtgt gttcagtagg ttttgatagg ctaacaagcc aaacgttctt
     7381 tgacaaccaa ataaaggcaa tcgaatacgt ttcggtaaga aaagagctga tgaactaaca
     7441 cgccatgacc tacaaaatta gtgagcgatt catgttagta attcgaaagt aatctttagc
     7501 aggattgctg ccatgacttt cttatcgtga taatgatact cccgtatagc catagtccga
     7561 gcgttagaag cgtcacaggc aatgggtacg gctacatgag aaccatgcag aagtgaaatt
     7621 cctgtgagct ttgctagagc tgttcgattg ctaagatatt cttctcttgt cagtgtatta
     7681 ttataaaact atttgatata attataggtg aatattggag ttttcctagc ctataattgg
     7741 gaaaggagta ataatgataa cggtcacaaa aaaggattta gttgagctag gatatggaac
     7801 ttctttttca gcagacatca taaaaaaagc aaaagaattg atgattacaa aagggcatat
     7861 ctactatcaa tctcgaaagc tagatagagt ccctcgtgaa gctgttgaag aaattctagg
     7921 aattcggttt tcaaatgaaa acaggtctga gtagtttttg cacagtaagg agtaaaaact
     7981 atggttaaag acccaataaa aaaagcaaag aatggaacct attattttcg tgcaaatcta
     8041 ggttatgatt ctaaagggaa aaaaattcaa aaatatcgta gtggattcac tactaaaaaa
     8101 gaagcaagag aagaatattc gaaactgcta ttgatgaaac cagaagaatt aagtgagaat
     8161 aaggataaaa tgacgtttaa acattatatc tttgaaattt tccttccatg gtataagaca
     8221 caggtaaagc tcagaacata tgaaaatcgc ttaccaacta ttaagaagca tttctcttat
     8281 tttgataaaa tggctgtttc cgatattgag cctattgatg tgcaaaattg gcaactaaaa
     8341 ttatcaaaga agtgtaagtc ttcttatgta agagctgttc aaggactatt ttcagttgct
     8401 atggatcgag caattgtcct tgggataacc accacaaatc catctaagat tattggcaat
     8461 gtgaaaaagc aaaagtctaa aattgaattt tggacaaaag aagaatttga aaaagtaatc
     8521 tctctaattt acaaagaaga ttactatcaa cactttttat ttatctctct gtggttttta
     8581 tttatgactg gaatgcgaat tggtgaagct actgccattc aatgggaaga tattgatttt
     8641 gactcaggag tactaaccat caacaaaaca ctctattaca agaatcaatc gaattataga
     8701 tttgttgaac caaaaacaaa ggcgagcgtt cgtcatattg tattagataa atgcacatta
     8761 acttatttat ccgaatggaa ggaagtacaa caaaatctaa ttcagacaga ctttgtaatg
     8821 agctataacg gtattcctac ccaaaaacat acactatcat atgctattga acgctatgct
     8881 aagctggctg gtatccatcg aatcagaatc catgggttac ggcattctca tgcttccttg
     8941 ttaatcagta tgggagaaaa tccacttatc attaaagatc gtctaggaca cgaagatatt
     9001 gagacgactt taggaacgta tggacatctt tatcctaaca gtaatttcga ggtagctaac
     9061 aaattaaatg gagccatttc ctttaaagaa gcaaaacaaa attatgatac ttcaccaaag
     9121 aatcaattta ctgtagacta cttgcgaaac aaagaaatga aaaaagtgca ataaaagtgc
     9181 agtaaaaaga taagaagagc cagaaaccct tggtataaga ggatttctag ctctcctaca
     9241 actattccca ctccactgtt gctggtggtt tagaagtgat gtcatacacg atacggttga
     9301 cgtgggctac ttcgtttacg atacgtacag agattttttg caagacttcc catgggatac
     9361 gggcaaagtc agcggtcatt ccgtcgattg aagttacagc acggataccg actgtgtagt
     9421 cgtaagtacg tccgtcaccc ataacaccga cagagcggat ccctggaaga acagtgaaat
     9481 attgccagat atcacggtca agtccagcag ctgcgatttc ttcacgcaag atagcatcag
     9541 attcgcgtac gatttctagc ttgtcttcag tgatttcgcc aagaacacga ataccaagac
     9601 caggaccagg gaatggctga cgccaaacga ttgaatcagg catgcctaac tgagtcccta
     9661 attcacggac ttcgtctttg aataatgtgt ttaatggttc gatcaattca aattgcatat
     9721 cttctggcag tccacctaca ttatggtgag atttgatcgt ctgtgctgtt tctgtaccag
     9781 attcgataac gtcagtatac aacgttcctt gtgctaggaa agagacgcct tcttcaccag
     9841 caagttttgt tgcttcgtca tcaaaaacat atacgaattc attcccaatg attttacgtt
     9901 tttgttctgg gtcagataca cctttcaact tgtctaagaa gcgatctctt gcatcgactt
     9961 tgatgatgtt caagccgaat tttccgccta gactttccat tacttgttcc gcttcgcctt
    10021 tgcgtagcag accgtggtca acaaagatag atgtcaattg gtcaccgatc gctttttgta
    10081 aaagaacccc aacgacacta gaatcaacgc cacctgaaag accaagcaag acttttttgt
    10141 caccaacttg ttcacggatt ttttctactt gcatatcgat aaagttatcc attgtccagt
    10201 taccttcaca gtgacaaaca tcaaaggcaa agtggcgcag caattcgatt ccgtattcgg
    10261 aatgacgtac ttctgcatgg aattggatac cataaaaatt acgtgttgtg tcttggatag
    10321 aagcaatcgg acaatctttg cttgttccga ctgtttcaaa gccttcagga actttagtta
    10381 ccaagtcacc atggctcatc catactgttt gttgttttgg tgtttcttta aacaagacag
    10441 cttgatcgct tgttacttct aacaaggctt tcccatattc gcggttttta gcaggctcta
    10501 cggaaccgcc aagtttgtac gtgatcaatt gcataccata gcaaatccct aatacaggaa
    10561 tacctaaatt gaagatctca ggatcgatcc caaatgcatt ctcatcatat acactgtttg
    10621 gtccgccaga aagaatgatt ccctttggat tcatcgcttt tacttcttct gcagtaatgc
    10681 ggtggctcaa taattcggaa aagactccaa gatcacgaat acgtcttgtg atcaattggt
    10741 tatactgact accgtagtct aatacgataa tcttttcaac gttcgtcata tcggcaacgt
    10801 ttgtcaaatt tattacccct atccctttaa attttatctc aaaatagtca aactatttta
    10861 aaatgctaat attttcgcat gaaaatcgaa tcgatgatat gattttcagt tttatggaga
    10921 attatatcgg ctcttcctcg tgtaggcaag atatattcct gtaaattttt caaattcaca
    10981 tttttccata catcttttgc cattttaaag gcttcttcac gatctccgat agcaaaacga
    11041 taataataat tctctggatt ttggaaagcc gtatccagta acgcaccaaa acgatccaaa
    11101 taccattttt cgatcagcac gggatcggca tcgacataga cagaaaaatc aaaaaagtcg
    11161 ctgacgtaga tttgctgatt cgcaggtaat tgaagcgtat tgatcccctc aacgatcaag
    11221 atatcaggct gctggatcaa ttcgtacttg tctggaatga tatcatacac atcatgtgag
    11281 taaacaggta cttttatctc ttcctgacca gattttacac ggttcaagaa atcgatcagc
    11341 atctccatat cataactttc aggaaatcct ttacggtcca tgattccacg ctcttttaat
    11401 atacgatttg gataaagaaa tccgtcagtc gtgatcagct gaacgtttct tcgtttgaag
    11461 gtacgagaca aaatcgtctg cagcagtctg gctgttgtac tttttccaac ggctacactt
    11521 ccggcgatcc cgataataaa aggcggtact tctacgtatt tatgaagaaa taaaccttta
    11581 ctcaagaaca aagattcaaa ttctttcata taaagataaa tcagatgagc aagaggtaca
    11641 tagatatctc gaacatcctg catggaaatg cggtcattca aacttttgat attatctagt
    11701 tcatcttgtg tcaaaggtgc ttgcccatta cgataaaatt ctcgccattc ttctcttgcc
    11761 acacggtagt agttcattgg atcattcata ttagcctcct gcgttctccc atcaatggaa
    11821 ctattttaac acaaaataaa cgcaaacccc accacttaag agagattgcc gatctttttg
    11881 tataatcttt ataatagaaa gaaacggagt cttattcttg acttgcgcac ggatacataa
    11941 aaggatagac tgttgtcatg aaagaaatga gaaggatgga agacatgaca aatcattact
    12001 attctgaacg cccagatacg gcgcacgatt ttgaacaatg ggcttttgag ttaaaaggca
    12061 gaacctttca atttgtaaca gattctggtg tattttcacg tgaaacagtg gattatggtt
    12121 cacgtgtcct gattgatgct ttcgattggg aagagttacc tgatgaagga aagatcttag
    12181 atgtgggatg cgggtatgga ccgattgggt tagctttggc atttgcgaca cagcggtttg
    12241 ttgaaatggt cgacattaat gaacgagcgg taagtctagc gcaaggaaac gcaaagcgta
    12301 accaaatcga acatgttgat atccatcaat cgaatatcta tgaagcagtt catgaagaaa
    12361 cctatgcagc tatcgtaagc aatccgccga tccgtgctgg aaaaaaagtt gtacatgaaa
    12421 ttttgacagg cgcatatcct cggttgaaga agggcggtac actgacaatt gtgatccaga
    12481 agaaacaagg agcgccgagt gcgcaaaaga aaatggaaga aacatttggt aatgcagaaa
    12541 tcgtcacaaa agataaagga tattatatca tcaaaagtgt gaaagaataa ctagctgaaa
    12601 aatagtggag cgacgaaaga gtggaactgg gcgtgcatgt acgggtacgt atcagttctt
    12661 tttttgttga gaagttttta aaaacaaaga actatttttt cttcaaaaag aaatagtagg
    12721 aacagagtcg ttgtttttta cgataaaata aataagatac agaacttgta tagaaggctg
    12781 ccaagtcggt agataaagga ggtttttatc aaaaaacata ctttcctttt ttctctattc
    12841 ttttcttggg ggttttggta cactgatagg gaatttagat agaagggagc caataggatg
    12901 aagctgacca tcagagatat cgcagaaatg gcaggcgtat cagtaacaac tgtctcacag
    12961 attttgaata acaaaggaag cagatttagt gataagacaa gaaaaaaagt gttgtcgatc
    13021 gtgaatgaac atcattataa gcccgattat ttcgcttcaa atgtgatcaa tcgacattca
    13081 aagaccatcg gaatgatcgt ccctgatgtc actgatttct tcttctccaa actgatcgaa
    13141 ggtgtagaga gctatttgaa tcctttgggc tatgtgatca tgttatgcaa ttctcgtcat
    13201 agccaagaaa atgagatcaa gtatttacaa gagctttccc accgctcagt ggacggaatc
    13261 ttgctggcca caccaaatat tttacctgag aaatatgcat tagacagcgg tttttatcaa
    13321 aaaatgccaa tcattttaat cgatcgcgga ttgaatcgtc gggataacgg acggcttatc
    13381 gtaaaggaat atgagggcgc ctatcaagct gtttcgctgt ttattgaaaa tggccatacg
    13441 aagatcggca tgctgaagga aaccactgga tactatcaat tagaagaacg tttcaacgga
    13501 tatcgtcatg cgctgaaaga tcatggcctg ccttttaatg ggaaatttgt tgaacatgga
    13561 gagttgactg tccaaggtgg atatactgca tcaaaaaaat tactcaaaca taaagatatc
    13621 acggctattt tttgcgggaa tgatgccatg gcgatcggat gctatcaggc gatcgatgaa
    13681 ttgggcaaaa aaatcccaga agatatctca gtcataggat tcgatggatt aaagctgtca
    13741 gaatacatga ttccgcaatt gacgacagtg cagcagccaa gttttgatat tggattttat
    13801 gctgcacgtt tcctgatcga tacgatcgaa tttcctcaaa gaaaagttcc gaacaaagtg
    13861 ttcgaaacaa aattaattat tcgtgagagc gtaaaagtat tgacaaaggg ctaactcggc
    13921 ggtatattgc ttatgtaagc atttacaaag gctagttagt gcttttttta tttttagtaa
    13981 accagtttac taaagtggtt tacctttcct aaaaataatg tataataccg aatgaagaca
    14041 aggaggatca ggcaatgcga atggtggatc tgattgaaaa aaagagggat ggaaacgagc
    14101 tgtcgaaaga ggaaatcgag tacatcgtca cgaactatac gaatggaaag attccagatt
    14161 atcaagtcag tgcattattg atggcaattt tttatcaaga catgacaaat gaagaaatca
    14221 cgaacttaac attggcgatt gctaattctg gtgatgtcat cgatttgagt tctcttgaag
    14281 gaataaaggt agataagcat tcgacaggtg gcgttggtga cacgaccaca ttgattttag
    14341 cccccctagt agctagtgtc ggagtgacag tggctaaaat gtctggacgc ggtctaggtt
    14401 acacaggtgg tacactagac aagctagaag caattcccgg atttcagatc gagctttcgg
    14461 atgaagaatt tgtacgcatt gttaatgaaa gtaaagtagc ggtaattggt caatcaggaa
    14521 acttggcacc ggcggacaaa aaattgtatg ccttgcgtga cgtcacagcg accgttgatt
    14581 ctcttccgct gattgccagt tcgattatga gtaaaaaaat cgcggcagga gcagatgcaa
    14641 tcgttttgga tgtgaccact ggagacggtg ctttcatgaa gaacatcgaa gatgcaagac
    14701 gtctggcaaa gaccatgacg agcattggga aactggctaa ccgagaaaca gtggcggtta
    14761 tctcagatat gtcagaacct cttggcgaag ctatcggaaa tagtctagaa gttgtcgagg
    14821 caatcgaaac attacaagga aatggtccag aagatctagt agaaatgtgc tacgccttag
    14881 gaagccaaat ggttgttctt gctggcaaag ctaagacaac cgatgaagca agaacgttgc
    14941 ttcaagaagc tctggaatca ggtaaagcgt tagcaaaatt caaagaaatg atccaaaatc
    15001 aagggggaga cccagcgatc gtggaacatc cagaacgaat tttgactgca cgctacacaa
    15061 tggagcttcc tgctaaacaa agcggagttg tgtcaaaaat cgtggcaaat gaattaggaa
    15121 ttgctgcaat gatgctaggt gctgggcgta aaaccaaaga agaagatatt gatcacgctg
    15181 ttggattgaa actgcataaa aaaattggtg acactgtgac aaaaggtgag tcactattga
    15241 cgatctatag taacgacgaa gaaatcactt ctgtcattga attattatat aaaaatattg
    15301 aaattggtga atcggcaatg aagccaacgt tgattcatga catcatcaca gaataaagga
    15361 aaaaagcgga ctttaccgtt ttaaagaaaa ggggaaaaga aatggaacta aaccgaatga
    15421 tcgaccatac tattttgaaa gcagacgcat ctaaagaagc tgtgatgcaa attatcgaag
    15481 aagcaaaaaa ataccatttt tattctgtct gcatcaatcc agcatgggtt gcgctagcga
    15541 aagaacaatt acaaggtgag ccagtagccg tatgtacagt aatcgggttt cctctaggag
    15601 caaacacacc cgagacaaaa gcatttgaaa caacagatgc catcaataac ggtgcagatg
    15661 aagtcgatat ggtcatcaat atcggtgctt tgaaatcaaa ggatgatcaa caagtccaaa
    15721 aagatatcga agcagttgtt gaagcagcaa aagatcgtgc gcttgtcaaa gttattattg
    15781 aaacatcgtt attagataga gaagaaatca tccgagcttg cgaaatagca aaagctgcag
    15841 gcgctgattt tgtcaaaaca tcaactggtt tctcaacagg cggagcaaag gtagaggatg
    15901 tccgtttgat gcgtgaaact gtaggaccag aaatgggtgt caaagcttcc ggcggtatcc
    15961 ataacgaaga agaagcaatg gcaatgatcg aagctggtgc tacaagaatc ggaacaagcg
    16021 caggagtagc aatcgtatct ggttcaactg gtgaaggcta ttaatttttt caaaggagaa
    16081 gctcatggca gaactcaaac aagaatggat caacacagct attgaagcgt tagataaagc
    16141 ctatgttcct tattcccatt ttccggtagg cgcttgtctc gtaacggaat caggcaagat
    16201 ttatcaaggc atcaacattg agaatgcatc atttggatta acgaattgtg ccgagcgcac
    16261 agcttttttc aaagcagtat ctgaaggaga aaaaacattt acgcatctag tcgtagcggg
    16321 gcacaccccc gatcctatct ctccttgtgg tgcatgtcga caagtaatgg cagaattctg
    16381 ttcccctgat atgccggtta cgctagtagg agacaatggt gtgacgaagg caacgaccgt
    16441 tcgtgcactg ctgccttatg catttactga aaaagatcta taactcatgt tcattcagtc
    16501 agctgactgt tgaaaaataa aacaattatt ttaggaggct ttacagaaat gaaaaaagca
    16561 aaattatttg gtttaggtgc tgtcgcacta gcagcagggt tatttttagg tgcatgcggc
    16621 aacaacggct ctactgattc atccggtggc aaaagctcat cagacacaac aactgcagca
    16681 ttgatcacag acacaggtgg tgtagacgac cgttcattca accagtcagc ttgggaagga
    16741 ctagaaaaat gggggaaaga tcaaggactt tcacgaggaa atgatggctt ccaatatttc
    16801 caatcttcaa atgaatcaga ttacatccca aatattgacc aagcattgaa tgctggattc
    16861 aaaacgattt ttggtattgg ttataaacta aaaccagcga tccaagaaca agcaacaaac
    16921 aatacaggta cgaactttgt tattatcgat gatgtcatcg atggattaga taacgttgta
    16981 tctgcaactt tcaaagacaa cgaagcttct tatcttgcag gtgttgcagc agcttatacg
    17041 actgaaacaa acgtagtcgg ctttatcggc ggtgtaaaag gtgaagtaat cgatcgtttt
    17101 gatgctggat tcaaagccgg tgttgatgca ggtgcgaaag aattaggaaa agaaatcaaa
    17161 gtattgaatc aatatgctgg tgacttctct gctccagata aaggacgttc aattgctcaa
    17221 ggtatgtatg cacaaaacgc agatatcatc ttccatgctt caggcggtac aggtaacggt
    17281 gtattccaag aagcaaaatc attgaacgaa tcaggcgaca aaaaagtttg ggttatcggt
    17341 gttgaccgcg accaatcaga cgaaggcgaa tacacattaa acggcgaaaa gaaaaacttc
    17401 acattgacat ctacactgaa agcagttgga acagtagtag aagatttggc acaaaaatca
    17461 gcggacggta aattccctgg cggcgaacac actgtttatg gattgaaaga agacggtgtt
    17521 ggtctaacag aaggacaact ttctgacgaa gcgaaaaaag cagttgatga agcaaaagaa
    17581 aaaatcattt caggtgacgt aaaagttcca gaaactcctg aagaaaacta aaaccatttc
    17641 tatagccttg ttagacacat gtttgacaag gctatggttt gattagaaca agaattgttc
    17701 atttatccaa gctgccttgt gacagtttgg ataagggaac aactccctga agtaatgttt
    17761 gtttcaaaag aagaacgctc actaagcgtg aatcttagtc aatcgttgcc ctagatagaa
    17821 atcgttagga tgtgaaaaaa aggtggttca agaaaatatt gtcatagaaa tgcagaatat
    17881 taccaaagaa tttggaacat tcaaggcaaa tgacaacatc aatttacaag taaaggcagg
    17941 agaaattcat gcgttgcttg gagaaaacgg tgctggcaaa tctacattga tgaacgtgct
    18001 ttccggatta ttagagccga catcagggaa aattttgatg cgtgggaaag aagtaaagat
    18061 cacaagtccg accaaagcca accaattagg gattgggatg gtccatcagc actttatgct
    18121 tgttgatgcc tttactgtaa cagaaaacat cgtgttggga agcgagccta gtcgcgcagg
    18181 gatgcttgac cataaaaaag cacgaaaaga gatccaaaaa gtttctgaac agtatggatt
    18241 gtcagtcaac ccagatgctt acgttcgtga tatttcagtt ggaatggaac aacgggtaga
    18301 aattttaaaa acactttacc gaggagcaga tgtactgatt tttgatgagc cgacagctgt
    18361 attgacccct caagaaattg atgaattaat cgtgatcatg aaggaattag tcaaagaagg
    18421 caagtcgatc attttgatta cgcataagtt agatgaaatc aaagcagtag ctgaccgctg
    18481 tacagttatc cgccgtggaa aaggaatcgg tacagtcaac gttaaagacg taacctcaca
    18541 acaattagcc gatatgatgg ttggaagaac ggtttcattc aaaacgatga aaaaagaagc
    18601 gaagcctcaa gaagtcgttt tgtctattga aaatctagtg gtgaaagaaa atcgtggatt
    18661 agaagccgtg aaaaacttga acttagaggt tcgtgctggc gaagtacttg gtatcgctgg
    18721 aatcgatgga aacgggcagt cggagttgat ccaagctttg actggtttgc gaaaggcaga
    18781 aagcggacat atcaagctaa aaggggaaga catcactaat aaaaaacccc gaaaaatcac
    18841 tgaacatggt gtaggacacg tgccagaaga ccgtcataaa tacgggttag tcctagatat
    18901 gacattatct gaaaatatcg cactacaaac ctatcatcaa aaaccttaca gtaaaaacgg
    18961 catgctgaat tattcagtaa tgaatgaaca tgccagagaa ttgatcgaag aatatgatgt
    19021 tcgaacaacg aatgaacttg ttcccgcaaa agctttatca ggcggaaatc agcaaaaagc
    19081 aattatcgct cgtgaagtcg accgagatcc tgatctgttg attgttgcaa atccaactcg
    19141 tggactggat gtaggtgcga tcgaatttat tcataaacgt ctgatcgaac aaagggacaa
    19201 atacaaagca gtgttactga tcagtttcga attagaagaa attttaaatg tttcggatcg
    19261 tattgctgtt atccatgaag gagaaattgt cggtatcgtt gatccgaaag aaacatctga
    19321 aaatgaatta ggtctattga tggcgggcta taccttagaa gaagcacgaa aagagttaga
    19381 aaagggggct ggcgcacatg aatgatcgca gcgaaaaatt ccgtaattta ttagtgcctg
    19441 tactttcagt tgttttagga ttgatcttag gcgcgatatt gatggctgct tttggttttc
    19501 agcccatcca aggttattct tctatgctag atgcagccct tggttcgcaa agagggatag
    19561 gtgaaacgtt aaggcaagca acgccgttga tttttacagc attaggtttt tctgtagcta
    19621 actcagctgg gtttttcaat attgggcttt ctggacaagc actttgcggg tggatcgtca
    19681 gtgtctgggt ggcacttgcg tttccagatc tgccaaaagt tgttttgctt ccgttatgcg
    19741 tgatcttagg tgccttagct ggtgcgatgg cagcggctgt tccaggttta cttcgagcat
    19801 tttttggaac aagtgaagtg atcgtaacaa tcatgatgaa ctatatctta ttatacgtaa
    19861 gcacatttgc tttacatgaa gtgatgcctg aaagctatcg ctcaagtttg gactcttcca
    19921 atatgatcag tcaaaatgcc tcactgcggt taccatggtt aacacaagct tttggcggct
    19981 ctcgcgtaaa ctcaggatta tttttagcaa tcatcgcgct tattttcgtt tggctgctga
    20041 tgaagaaaac gactttagga tttgaaatcc gttctgttgg attgaatcct tttgcttctg
    20101 agtatgcagg aatgagtagt aaacgtacga tcgttttgtc gatggtcatt tctggcgctt
    20161 tggcagggct tggcggtgtg gttgaaggat taggaacgta tcaaaacttc tttgttcaaa
    20221 cgacttcgct atctattgga tttgacggaa tggctgtttc cttgcttggt tctggatcag
    20281 caatcggtat tcttttatca gcgatcttgt tcagtatttt gaagatcggt ggacttggca
    20341 tgcagacagg agcaggtgtt ccgtttgaga tcgtcaatgt atcgattgct ttgattatct
    20401 tctttgtcgg gatcaacttc ctgattcgtt tcgtcatggc taaagtcttc caagggaaaa
    20461 aacaagaaga gatcgttgcg acaatagaat cgaaaccaac gaatcaaaac catgaagggg
    20521 gcgaactata atgtcgacta tcgaattaat tgccttgatc cttacttcta cactggttta
    20581 ttcaacaccg ctaattctaa cgtcactagg cggaactttt tctgaacgaa gcgggatcgt
    20641 aaacgtaggg ctagagggaa tcatggtcat gggtgctttt agttccatcg ttttcaactt
    20701 gacgatgagt ccatcacttg gttctatgac accatggatc gctattttag tgggtggcgt
    20761 cgtaggtatc ctattttctc tgattcacgc agtagcaaca gtcagtttgc gtgctgacca
    20821 tattatcagc gggacagtaa ttaatctgat ggcacctgct ttaggtgttt tcttgatcaa
    20881 agcatggtat ggtaaaggtc aaactgataa catcacgcag aatctggggt atttttcttt
    20941 cccaggatta gcagatattc cggtcattgg accaattttc tttaaaaata cattcttacc
    21001 tgcttattgt gctattttga ttgctgtcct agcttgggtg atcatcttca agacgaaatt
    21061 cggcttgcgc ctccgttcag ttggtgaaaa tcctcaagct gccgacacat taggtatcaa
    21121 tgtatatgct atgcgttatt caggcgtact gatttctggc ttgttaggcg gtatgggcgg
    21181 tgctgttttt gctcagtcga tttccggtaa cttttcagct ggtacgatcg ttggacaagg
    21241 gttcatctcc atggctgcta tgatctttgg taagtggaat cctttagggg caatgggtgc
    21301 ctcattgttc tttggttttg ctcaaagctt gagtatcatt ggcgcacaat taccagttat
    21361 ttcttcgatt ccgccagtat tgctgcaaat tgctccatat ttgctgacaa tcattgtgct
    21421 ggttgttttc ttagggaaag cttctggtcc aaaagcaaac gggaagaact atatcaaatc
    21481 aaaataatct tttagcgaat aaaaagggtg ggttacggga agtgtttttc gtctcgccct
    21541 ttttgtttta aaggcaataa aaataaaaag aaagaaggaa taatcatgtt taaacgcgta
    21601 catctgatcg tcatggattc agtaggaatt ggagaagcac cagatgccga taagttcggt
    21661 gatgttggtt ctgatacatt aggacacatt gcgaaagaag caggattgaa cgttccacat
    21721 ttagagcagc ttggtttagg aacgatccgt cctcttgaag gtgtggaatc agtgaaagat
    21781 catcaaggct atgcgacgaa attagaagaa gtttctgtag ggaaagatac gatgacaggc
    21841 cactgggaaa tcatgggatt gaatatcaaa actccatttc gtgtgtttcc taatggattc
    21901 cctgatgatc tgatcaaaca aatcgaagaa ttttctggcc gtaaagtcgt atgtaacaaa
    21961 ccttatagcg gaacagctgt tattgacgat tacggtgaac accaaatgaa aacaggcgat
    22021 ttaattgtat atacttctgc cgatcctgtc cttcagatcg cggcacatga agaaatcatt
    22081 cctttagaag aattatatcg gatttgccaa ttcgttcgtg atataacaaa agatgatccg
    22141 tatatgattg gccgaatcat tgcacgtccg tacgtgggtg aaccaggaga cttcacacga
    22201 acaagcaatc gccatgatta tgcgttgaat ccattcggac acacagtcct tgattctttg
    22261 aaagaagcag gaaaggacgt gattgctgtt ggaaagatca acgatatttt caacggacaa
    22321 ggaatcactg aatctgtccg cacgaaaagc aatatggatg gcgttgacca gctgctgaaa
    22381 gttatgaaaa acgatttttc aggaatcagc tttaccaatt tagtagattt tgatgcatta
    22441 tacggtcatc gtcgggatgt agtcggctat gcgcatgcga ttgaagaatt tgacttacga
    22501 attccagaat tgcttgaagc gatggaagaa gacgacttgc taatgattac agccgatcac
    22561 ggaaacgatc cgacgtttac tggtaccgat catacgcgag aatatgtacc gttattggtt
    22621 tacagtaaaa aaatgaaaga acaaggggca ttgcctcaag gatattattc agatatttca
    22681 gcaacgattg ctgagaactt tagtgtttct tcgactgaaa atggcgaaag cttcttgaaa
    22741 ttattaaaat aatcttttta aataggagaa agcaaatgac caagttaagc gagatgttac
    22801 aagaaacgac ccagtttatc cttgataaag gagtaaaaga aattgatttt ggtttgattc
    22861 taggttcagg attaggtgaa ttagcagatg aaatcgaaga tgctgtaatg atttcttatg
    22921 atgagatccc gttctttcct gtttcgacag tagttgggca tgcaggaaag ctagtttatg
    22981 ggtctctggc aggcaagaaa gtgttagcca tgcaagggag attccactac tatgaaggtc
    23041 attccatgca aacagtgacc tatccagtac gtgtgatggc cggactaggg gcccattcag
    23101 tcattgtgac taatgcatgt ggtggtgtca acgaatcatt tgtaccagga gatttgatgc
    23161 tgattaccga tcatatcaat tttactggtg aaaatccttt gatcggtccg aatgaagatg
    23221 caatgggccc tagatttcca gatatgagtc aagcttatac gcctgactat caagcaacag
    23281 caaaagcagc agcagaaaaa ctaggtttgg atcttaaaga aggcgtatat atgggctatt
    23341 ctggaccaac ttatgaaacg cctgcggaga tccgaatgtc tcggacaata ggagccgatg
    23401 cagtagggat gtcgactgtt ccagaagtca tcgtagcagc tcacagcgga ttaaaagtgt
    23461 taggaatctc ttgtattacc aacttagcag caggtatgca agcgaatctg agccatgaag
    23521 aagttgtaga aacaactcag agagtcaaac aatcttttaa agcattggtt aaagaaacat
    23581 tgacattatt gtaataggag gaacaataaa atgagtgtgc atattgaagc gaaagaaggc
    23641 gagatcgccg ataagatttt acttccggga gatcctttgc gagctaaata tattgcggag
    23701 acttttttgg aagatccagt ttgttacaat caggtacgcg gtatgttagg ctttaccggc
    23761 aaatataaag gagaacgtat ttctgttcaa ggaacaggaa tgggaatgcc atcagccacc
    23821 atctatgccc atgaattgat ccagtcgtat ggtgtaaaga aattgattcg agtaggtaca
    23881 tgcggtgcat tgtcaaaaga cgtccatgtt cgtgatttag tactagcaca aggtgcagcg
    23941 accagttctt cgatgatcga aaaaaatttc caagcctttc attttcctcc aatcagtgat
    24001 ttcaatctat tactgaaagc atatgagata gctaaagaaa agggctatac ggttcatgta
    24061 ggaaatgttc tttcggaaga ttctttctac aaagatgatt tgacagagac gttccaatta
    24121 gctgaacttg gcgttttagg agtggaaatg gaagcagcgg ctctttatta cttaggcgcg
    24181 aaataccatg tgcagacatt aagtttgatg accgttagcg accatttgat caccggtgaa
    24241 gaaacaacag ctgcagaacg tcaatctacg ttcaatgaaa tgatcgaagt aggattggaa
    24301 acagcgatag cagaataaga aggaaatgaa aaagctgccc tgcattaagt aataagaaca
    24361 tccaagcaaa aatagctcct catgtttttg acttggctgg acttattatt gcaaatgtaa
    24421 gcttttgaac accgtttacc aagaggttgt gaatctgccg tttaactctg agtcacaagg
    24481 aacaatttca aaaaacagct tttcatgttt gatgaatatt gtgacttgtg accgagaagt
    24541 tggcaggaga aaaccgttta tacgaaaaaa gggactgtga caaatcgtgt cacagtccct
    24601 tttttatagt gttttttata ggtagtattt tttagcaact gttaagtcat catttaattc
    24661 ataaacaagt ggttgacctg ttggaatttc aagatccatg atgtcttcgt cagagatacc
    24721 ttcgatatgt ttagctaggg cgcgtaatga gttaccatgt gcagctacta aaacagtttt
    24781 gttgtctagt aatgctggag caatttcatc ttgccagaat ggcaaagcgc gttctaatgt
    24841 cactttcaag ttttctccac caggaatatc gcgtttgtct aacattgcgt aacgacggtc
    24901 atttgctgca gaaccttcgt cagtttcttc cattaatgga ggaagagtat cgtaagaacg
    24961 acgccagatg tgcacttgtt cgtcgccgta tttttcagct gtttcttttt tgttcaaacc
    25021 ttgtaattta ccatagtgac gttcgtttaa gcgccatgat ttgatttgtg gtacccaaag
    25081 ttgatctgaa tattctaaga tcaagttaca tgttttgatt gcacgagtta aaacagaagt
    25141 gtaagcaaca tcaaattcga ttccagcttc tttgattttg cgtccgcctt cttttgcttc
    25201 ttcgattcct tctggtgcta gatttacatc agcccaacca gtaaattggt tcaatgcatt
    25261 ccattcacta agaccgtgac gagaaaaaac taattttggc atgtttagct ctccttttaa
    25321 gataggttta tatttctgaa attttcagtt ctgttttatt gtacaatttt ttggaagaga
    25381 tttccatcga aaaattagat aagattctat gagaaaacaa gtagagaata taaaaaaacg
    25441 aacagtgccc acgcaactgt tcgtttttag gtattagatg cataattgct cgatttcttt
    25501 ttcaggtaaa ttgatatatt tataacatgt tccaactgaa acaccgcatt ttgaagaaat
    25561 aaattggatc gtttcttttt tttcataata gagacgtcgg atttttttga tcgtttttgc
    25621 atcgattttt ggtcttccgc cgatttttcc ttttttacga gcttcatcta atccaactaa
    25681 tgtacgttct ttgatcaatg cgcactccat gtttgctagg ttttccatta attggaaata
    25741 gatttttccc atttcgcgcg tatcgattcc ttcatcaaga ctaatcaagt gcagcccgcg
    25801 atctttgaat aattgcgtga gttctgttaa ttgccgcgta gatttgccta atcgatgaag
    25861 ttggcaaatg actagtgcat cgccttgttg catgtcagcg atcactgact ccaaatgaac
    25921 agcagtagaa tcattttgtt ccacttcata tgtctcatgg aaaatttctt gacaaccaaa
    25981 gtcagataat tgatcaacct gagttgttaa atcaacgtca tttatggttg tgcgtgcata
    26041 accaataatt ttcatggtga ttcctttcta gcagtgattt tctaactgga tataacagta
    26101 aaaatgtagc ataaagtacg tcaaaaagat agttttttcg caaattttcc gctgtctttt
    26161 tttattggat gatcaagcag tttttcttta tttacgacgg attgcgtcaa gaatctcaac
    26221 gcctttttct gagccgacaa ctactttaga aaccatgttt aggaaaagcc catgttctac
    26281 taccccaaca agttcatcta aataggtagc taagctttct ggctgttcga tgacttctag
    26341 atgcagatca atgatgtaat gaccaccatc tgtggttaag acttctccgt tttcgtctgt
    26401 acgcaagacg ggttggaatc ttttttcttc aaaaatatga agtagctgtt gggagccata
    26461 agggatcact tcaactggga gtgggaactt cccaagttta tcgactaatt tagaagaatc
    26521 aacgatccaa atcgtttctt ttgagtacgt agcgactatt ttctcgaaaa gcaaagcagc
    26581 gccgccgcct ttgatccctt gaaaatcagc actgatttca tcagcaccgt ctattgtcaa
    26641 atcgactacg gggacttcat caatactctt taacggtatt cctaattttt ccgcttgttc
    26701 ttttgtcgca tttgaagtag taactcctgt gatacgtaaa ccttcttcac gcatacggcg
    26761 accaatctct tctaccatat aataggcggt tgaaccggtc ccgagaccta cgatcatccc
    26821 gtcctttacg aatttcgcgg cttcaatgcc agctaattgt tttaaattca tctttgcgtc
    26881 cacctttttt tagtttgagc atcgtgcttt cctcactatt ttagcgtgat ttacggtaaa
    26941 aagataccat tcttttttat tccaataaag ctttttcaaa aaaaattgca tctcttgttg
    27001 acatgttttt tcattcgtga tattctaaca aaggtgcttt atctacaggt ttctgtgaag
    27061 aaacgtgctg actgtttatc aaagcatatt tgataaggat gcagacattg catttatttt
    27121 ttatcacaaa aaaagaacca cctggatgtg tggaactaga cgcaaatcgt gggaaggagg
    27181 aaacaaggaa tgcctacaat taaccaatta gtacgtaaac ctcgtaaatc aaaggtggaa
    27241 aaatctaatt cacctgcttt gaacaaagga tataacagct ttaagaaaac tcaaactaac
    27301 gtaaactcac ctcaaaaacg tggggtagca actcgtgtgg gtacaatgac acctaaaaaa
    27361 ccgaactcag ctttacgtaa atatgcacgt gttcgtttgt caaacttaat tgaagttact
    27421 gcttatatcc cagggattgg tcataactta caagaacaca gtgttgtatt attacgcggt
    27481 ggtcgtgtaa aagacttacc aggggtacgt taccatatcg ttcgcggtgc tttagatact
    27541 gctggtgtta acgaccgtaa acaaagccgt tctaaatacg gaactaaaaa acctaaagct
    27601 taattttaat tattaaacaa atgaaacttg cttctactat atagaagtaa ttactatgaa
    27661 caatatttcg gaaggaggag ttacggatgc cacgtaaagg tcctgttgca aaacgtgatg
    27721 ttttaccaga tcctatttat aactcaaaat tagtaactcg cttgatcaac cgtgtaatgg
    27781 ttgacggaaa acgcgggatt gcagctaaca ttatctataa tgcctttgac ttgatcaaag
    27841 aatctacagg taacgatcca ttagaagtgt ttgaacaagc aatgaaaaac gttcaacctg
    27901 ttcttgaagt taaagcacgt cgtgttgggg gttcaaacta ccaagtacca gttgaagttc
    27961 gccctgaacg ccgcgttact ctaggtcttc gttgggtagt aaactacgct cgcttgcgcg
    28021 gtgaacacac aatggaagaa cgcttagcaa aagaaatcat ggatgctgca aacaacacag
    28081 gtgcttctgt taaaaaacgt gaagacactc ataaaatggc tgatgccaac cgtgcgtttg
    28141 ctcactatcg ctggtaagat tctctctgtt tggtcggctt ttataagtcg acctcagata
    28201 gtgtccttat ttttagcaac tagatagtaa agagaggaga aacaaggaga atgaccagag
    28261 aattttcttt acaaaacact cgtaatatcg gtatcatggc ccacatcgat gctggtaaaa
    28321 cgactgctac tgaacgtatc ttgtactata ctggtaaaat ccataaaatt ggtgaaactc
    28381 atgaaggagc ttcacaaatg gactggatgg aacaagaaca agaacgtggt attacgatca
    28441 cttctgctgc tactacagca caatggaagg gtcaccgaat caacatcatc gacacaccag
    28501 gacacgtaga cttcacagtt gaagttgaac gttcattgcg tgtacttgat ggtgctgtaa
    28561 ctgttcttga tgcacaatca ggtgttgaac ctcaaactga aacagtttgg cgtcaagcaa
    28621 caacttatgg tgtacctcgt atcgtatttg caaacaaaat ggacaagatc ggtgctgact
    28681 tcttatattc agtatcaact ttacatgacc gtttgcaagc aaacgctcac ccaatccaat
    28741 taccaattgg tgcggaagac gacttcacag gaattatcga ccttgtaaca atgaaggctg
    28801 aaatgtatac aaacgatctt ggaacagaga tcgaagaaac agaaattcct gaagaatacc
    28861 gcgaattagc tgaagaatgg cgcgaaaaat tggttgaagc tgtagctgaa actgatgaag
    28921 aacttacttt gaaatactta gaaggtgaag aaatcactga agctgagtta aaagaaggta
    28981 tccgccgcgc aacagttaac gtagaattct acccagtact atgtggttca gcatttaaga
    29041 acaaaggggt tcaattacta ttggacgctg ttcttgatta cttgccatca ccgttagata
    29101 tcccagctat caaagggatt gaccctaaaa ctgatgaaga agtagaacgt cctgcagacg
    29161 acagcgctcc tttctcagct ttagcattta aagttatgac tgacccattc gtaggtcgtt
    29221 taactttctt ccgtgtgtac tctggtgttt tacaatcagg ttcatacgta caaaacgcaa
    29281 caaaaggcaa acgtgaacgt gttggtcgta tcctacagat gcatgccaac tctcgttcag
    29341 aaatctctga agtttacgct ggagatatcg ctgctgctgt tggtttgaaa gacacaacta
    29401 ctggggacac actatgtgat gaaaagagtc tagttattct tgaatccatg gaattcccag
    29461 aaccagttat ccaagtcgct attgaaccta aatcaaaagc tgaccaagat aaaatgggtg
    29521 tggctttaca aaaactatct gaagaagatc ctactttccg tgctgaaaca aacgtggaaa
    29581 caggcgaaac aatcatcgct ggtatgggtg aacttcactt agatatcatc gttgaccgta
    29641 tgcgtcgtga attcaaagtt gaagctaacg taggtgcgcc tcaagtatct taccgtgaaa
    29701 ctttccgtgc tggcactcaa gccgaaggta agtttgtacg tcagtctggt ggtaaaggtc
    29761 aatacggtca cgtatggatt gaatttacac caaatgaaga aggtgctggt ttcgaatttg
    29821 aaaatgcgat cgtcggtggg gttgttcctc gtgaatacat tccagctgtt gaaactggtt
    29881 tgaaagatgc tatggaaaat ggtgtcttag ctggctatcc attagtagat atcaaagcta
    29941 aactttacga tggttcatac catgatgtcg attcaaatga aactgccttc cgtgttgctg
    30001 cttctatggc attacgtgca gcagctaaga aagctaatcc agtaatcctt gaaccaatca
    30061 tggctgtaga agttgttatc cctgaagatt atttaggtga tgttatgggt cacgtaactg
    30121 ctcgtcgtgg acgtgttgaa ggtatggaag ctcgcgctgg cggccaacaa gttgttcgtg
    30181 cgatggttcc acttgctgaa atgtttggtt atgctacaac tcttcgttct gctactcaag
    30241 gtcgtggtac atttacaatg acatttgacc actacgaaga tgtaccaaaa tcagtacaag
    30301 aagaaatcat taagaaaaac ggcggaaaag ctgaataatt tcttaaaacc ggatagtcct
    30361 gcaaagagat tatccaaaga gactatattt ttaatatagt ttcgtgtaaa ataattatcg
    30421 atgatatggg catgtggtgt accttgtgta catcatatac ctatcagaca aataaaaaat
    30481 atattgtatt tttaggagga cattttaaaa tggcaaaaga aaaatttgac cgttctaaac
    30541 cacacgttaa cattggtact atcggacacg ttgaccatgg taaaactaca ttaacagctg
    30601 caatcacaac tgtactatct aagaaaaacg gcggccaagc tatggcttac gatcaaatcg
    30661 atggtgctcc agaagaacgc gaacgcggaa tcacaatctc aacagctcac gttgagtatg
    30721 aaacagatac acgccactac gctcacgttg actgcccagg acacgcggat tacgttaaaa
    30781 acatgatcac tggtgctgct caaatggacg gagctatctt ggtagtttct gctgctgacg
    30841 gcccaatgcc tcaaactcgt gaacacatcc tattgtctcg tcaagttggt gttccttaca
    30901 tcgttgtatt cttgaacaaa gtagacatgg ttgatgacga agaattacta gaattagttg
    30961 aaatggaagt tcgtgaccta ttaacagaat acgaattccc tggtgacgat gttcctgtag
    31021 ttgctggatc agctttgaaa gctctagaag gcgacgcttc atacgaagaa aaaattcttg
    31081 aattaatggc tgcagttgac gaatacatcc caactccaga acgtgacaac gacaaaccat
    31141 tcatgatgcc agttgaagac gtgttctcaa ttactggacg tggtactgtt gctacaggtc
    31201 gtgttgaacg tggacaagtt cgcgttggtg acgaagttga agttgttggt attgctgaag
    31261 aaacttcaaa aacaacagtt actggtgttg aaatgttccg taaattgtta gactacgctg
    31321 aagctggaga caacattggt gctttactac gtggtgttgc acgtgaagac atccaacgtg
    31381 gacaagtttt agctaaacca ggtacaatca cacctcatac aaaattctct gcagaagtat
    31441 acgtgttgac aaaagaagaa ggtggacgtc atactccatt cttcactaac taccgtccac
    31501 aattctactt ccgtacaact gacgtaacag gtgttgttga attaccagaa ggaactgaaa
    31561 tggttatgcc tggcgacaac gtaacaatgg aagttgaatt aatccaccca atcgctatcg
    31621 aaaacggtac taaattctca atccgtgaag gcggacgtac agttggtgcc ggtgtcgtta
    31681 cagaaatcaa agcttaattc acttaagttt taaagtaaag caacatttat tcggacgtaa
    31741 tcgctcggaa cgaaggtctt aggatctttg ttccgagctt tttt
//
LOCUS       DAZKCZ010000025        29686 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000025 DAZKCZ010000000
VERSION     DAZKCZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.025"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..1223)
                     /locus_tag="ACG8KN_001010"
     CDS             complement(120..1223)
                     /locus_tag="ACG8KN_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFP9188084.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1852..2322)
                     /locus_tag="ACG8KN_001011"
     CDS             complement(1852..2322)
                     /locus_tag="ACG8KN_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFP9188085.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            2652..4292
                     /locus_tag="ACG8KN_001012"
     CDS             2652..4292
                     /locus_tag="ACG8KN_001012"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFP9188086.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            4446..5768
                     /locus_tag="ACG8KN_001013"
     CDS             4446..5768
                     /locus_tag="ACG8KN_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFP9188087.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5836..6270)
                     /locus_tag="ACG8KN_001014"
     CDS             complement(5836..6270)
                     /locus_tag="ACG8KN_001014"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9188088.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(6335..7171)
                     /locus_tag="ACG8KN_001015"
     CDS             complement(6335..7171)
                     /locus_tag="ACG8KN_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9188089.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(7913..8539)
                     /locus_tag="ACG8KN_001016"
     CDS             complement(7913..8539)
                     /locus_tag="ACG8KN_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188090.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8834..9169)
                     /locus_tag="ACG8KN_001017"
     CDS             complement(8834..9169)
                     /locus_tag="ACG8KN_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9188091.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(9156..9440)
                     /locus_tag="ACG8KN_001018"
     CDS             complement(9156..9440)
                     /locus_tag="ACG8KN_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9188092.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(9496..11019)
                     /locus_tag="ACG8KN_001019"
     CDS             complement(9496..11019)
                     /locus_tag="ACG8KN_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9188093.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(11012..12187)
                     /locus_tag="ACG8KN_001020"
     CDS             complement(11012..12187)
                     /locus_tag="ACG8KN_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9188094.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(12191..12376)
                     /locus_tag="ACG8KN_001021"
     CDS             complement(12191..12376)
                     /locus_tag="ACG8KN_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188095.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(12342..14036)
                     /locus_tag="ACG8KN_001022"
     CDS             complement(12342..14036)
                     /locus_tag="ACG8KN_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9188096.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(14033..14506)
                     /locus_tag="ACG8KN_001023"
     CDS             complement(14033..14506)
                     /locus_tag="ACG8KN_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFP9188097.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14575..14730)
                     /locus_tag="ACG8KN_001024"
     CDS             complement(14575..14730)
                     /locus_tag="ACG8KN_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFP9188098.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14864..15244)
                     /locus_tag="ACG8KN_001025"
     CDS             complement(14864..15244)
                     /locus_tag="ACG8KN_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9188099.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(15248..15463)
                     /locus_tag="ACG8KN_001026"
     CDS             complement(15248..15463)
                     /locus_tag="ACG8KN_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188100.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(15466..15873)
                     /locus_tag="ACG8KN_001027"
     CDS             complement(15466..15873)
                     /locus_tag="ACG8KN_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFP9188101.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(16139..17614)
                     /locus_tag="ACG8KN_001028"
     CDS             complement(16139..17614)
                     /locus_tag="ACG8KN_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFP9188102.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17604..18452)
                     /locus_tag="ACG8KN_001029"
     CDS             complement(17604..18452)
                     /locus_tag="ACG8KN_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFP9188103.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(18489..18851)
                     /locus_tag="ACG8KN_001030"
     CDS             complement(18489..18851)
                     /locus_tag="ACG8KN_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188104.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(18852..19010)
                     /locus_tag="ACG8KN_001031"
     CDS             complement(18852..19010)
                     /locus_tag="ACG8KN_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188105.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(19151..19775)
                     /locus_tag="ACG8KN_001032"
                     /pseudo
     CDS             complement(19151..19775)
                     /locus_tag="ACG8KN_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19772..20083)
                     /locus_tag="ACG8KN_001033"
     CDS             complement(19772..20083)
                     /locus_tag="ACG8KN_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188106.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(20127..20420)
                     /locus_tag="ACG8KN_001034"
     CDS             complement(20127..20420)
                     /locus_tag="ACG8KN_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188107.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20614..21261
                     /locus_tag="ACG8KN_001035"
     CDS             20614..21261
                     /locus_tag="ACG8KN_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9188108.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            21322..22467
                     /locus_tag="ACG8KN_001036"
     CDS             21322..22467
                     /locus_tag="ACG8KN_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9188109.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22548..22853)
                     /locus_tag="ACG8KN_001037"
     CDS             complement(22548..22853)
                     /locus_tag="ACG8KN_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFP9188110.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(22878..23309)
                     /gene="ruvX"
                     /locus_tag="ACG8KN_001038"
     CDS             complement(22878..23309)
                     /gene="ruvX"
                     /locus_tag="ACG8KN_001038"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9188111.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(23306..23572)
                     /locus_tag="ACG8KN_001039"
     CDS             complement(23306..23572)
                     /locus_tag="ACG8KN_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFP9188112.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(23827..24552)
                     /locus_tag="ACG8KN_001040"
     CDS             complement(23827..24552)
                     /locus_tag="ACG8KN_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFP9188113.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(24913..24983)
                     /locus_tag="ACG8KN_001041"
     tRNA            complement(24913..24983)
                     /locus_tag="ACG8KN_001041"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24949..24951),aa:Gly,seq:ccc)
     gene            25100..25852
                     /locus_tag="ACG8KN_001042"
     CDS             25100..25852
                     /locus_tag="ACG8KN_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9188114.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            25868..26347
                     /locus_tag="ACG8KN_001043"
     CDS             25868..26347
                     /locus_tag="ACG8KN_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9188115.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(26598..28178)
                     /locus_tag="ACG8KN_001044"
     CDS             complement(26598..28178)
                     /locus_tag="ACG8KN_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFP9188116.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(28171..28914)
                     /locus_tag="ACG8KN_001045"
     CDS             complement(28171..28914)
                     /locus_tag="ACG8KN_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188117.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(29090..>29686)
                     /locus_tag="ACG8KN_001046"
     CDS             complement(29090..>29686)
                     /locus_tag="ACG8KN_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9188118.1"
                     /translation="FLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVINR
                     KITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHYDE
                     ILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSI
                     SRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         8712 a         6019 c         4956 g         9999 t
ORIGIN      
        1 aaacaatatt tcaattttat tttttcgttt caaattattc tattagtgac atccttgaac
       61 tataaaaata ggagtacggc atcatctgcc gcactcctat ttttttcgtc atttatttct
      121 taaataaaac gattctcacg caattttatt tttttgatat cttctactgt ttgtgttcca
      181 gctaattgca tgaccatttc caattctgtt ttaaagaaat caaacacttg atggactcct
      241 gtacttcctc ctaaagacaa tccataaata acgggtctgc caatagcaac aagatcggca
      301 cctgatgcga ttgctttgaa aacatgttgt ccacgtcgta caccgctgtc aaacacgatt
      361 ggtacacgtt tatctactgc ttctgccaca tattgcaacg aatcaaacgc agcaggcccc
      421 ccatctaatt gacggccacc atggttagat acccaaattc caccagcacc agattctaat
      481 gatcgataga catcttcttc tgattgaaca cctttcacgt agactggcaa atcagaatga
      541 gcagcgataa attctacatc ttttggacta agtttttgtt tggaagattt atatacagca
      601 tccatcgttt ggcctactcc tgattgataa gcctgaacga tcggcattgg cagcgggaag
      661 gtaaacccgt ttctgcggtc tgtttcacgg tttccgccaa ctgttgcatc cgctgtcaat
      721 acgattgctt ttgctccatt acgcttggcc acttcaagaa tatccaaatt gattccatta
      781 tctttgctca tataaaattg aaaccattgc ggtgctttct ccccgcctgc ttctcttatc
      841 tcttctaaag tacaagaagc gtaagaactt gctgtataga tcgtcccaaa atctgctaca
      901 ccttttgcag aagctttctc cgccttcaca tgagctaatc catgagcagc aactggtgcc
      961 atgatgattg gtgcagtcag catttcttcg tcaaaatgtg tagttgtatc gggtaattct
     1021 acatctctta atacatgagg aataatcaaa cgatgattga atgcccgttc attttcttga
     1081 taagtaaata agtcgccagc accactgctg atgtaaccat aaccaccagc tggaatcact
     1141 tgtgaagccg cgatttctaa atcttccata ttgataaaat ctactattcc ttcatttgta
     1201 cctgcttgat atgttttttc catctcaatg ccttctctct ataaaatttg aatgatgaac
     1261 aatatgatag cgtttacact ttttatatta ctcttcgaca ttgatacagg caaataatct
     1321 gtttacattt cagccattcc agatgtcact actctggaag aaatgattgt aataaaaata
     1381 gataaaaaac cgatgggact ttgacaaaag tagtgtcaaa gtcccttttt ttgaataaac
     1441 ggtgttcaaa agccattttc tcggtattaa gtcaaattta tgcaaaaatg tggaaagcca
     1501 ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac ctctcgataa
     1561 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aatggcaaaa tatgaggaac
     1621 tattttgcca tttgtctctc taatactcag gtcagaacgt tgacttcaca gtctcttcct
     1681 taaacggtgt tcaaaaactt gcatctgcgg taataagccc aaccaaacga aaaataggag
     1741 gagctatttt tgtttggctg ttcttattac ttgatgcagg gcaggttttt cacgacctct
     1801 gattcacggt gtttcttttt catcaacatt tttacgaaat actgtggaag actattgaaa
     1861 taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc cattcttcat ttaattttgg
     1921 attaccgttt tcttccttga actcataagg tcctaattca aaaatcccag acagtatttg
     1981 attcacgatt tcatatagtt cagtgtgatc agacacatga ttcttgaaga tcatctcatt
     2041 atcaccacgt ttgaacctca cattcgggct ctcatgatga acctctatgc atttttctcc
     2101 tttttttaac tgacggactt ctgaaaacat ctcataaata tcttctccag taaaaccttc
     2161 agcaagcgcc agactgtcca caagaccgcc tactgtcagt tcagagatta ttgcttgatt
     2221 ggaatctaat tttaatgtat agcttgtcgc ttctactacg ccacatgcat tgcaaccgcc
     2281 ttctgatagt tcgctttttc taatcaattg aacttctttc attttctctt cctcctctta
     2341 attttctact tatgtatttt tatcacctga cataggtgac tcctatcaac gctatcatgt
     2401 gcttttttcc tcagaaatcc tatttatcca ttccatctat cccttattct tctacttttc
     2461 tttctctagc atataagaga tacgcatagt attcaagcaa ggattgtcga tataaaaaga
     2521 acaaatactg ctattttgat ttcttttggc tatattacat ataaataaaa tttatcatac
     2581 ctgctctttt tgactatatc atcatctgac ttctatactt cgtttgtatt aaaacttagg
     2641 aggaatcatt catgcttaca gggttaaact tattaaataa tccttttcta aataaaggaa
     2701 cagcttttac aaaagaagaa cgggcaaaat atggaatcac tggtatgctt ccaagtactg
     2761 tccaaacact ggaacaacaa tctgttcaag cttatggtca atacttaagc aaacaaacag
     2821 atttggaaaa acgtatcttc ttaatgaatc tattcaatac gaatcggaca ttgttttata
     2881 aattgatggg cgaacattta gttgaattca tgcctgtcgt ttatgatcct gtcgttgcgg
     2941 attcaatcga acaatacaac gaaattttct tgaaaccgca agatgctgca tttctttcaa
     3001 tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc ggcagatgga cgagacattc
     3061 gtttgattgt tgtgactgat gcagaaggaa tcttaggtat gggcgactgg ggtgtgaacg
     3121 gtgttgacat cgctatcggc aaattgatgg tctatactgc tgctgctggt attaatccag
     3181 cacaagtttt gcctgtatcg atcgacgctg gtactaataa tgaagaatta ttgaataatc
     3241 ccttatactt aggcaatcgt catgctcgtg tagaaggcga aacttactat gaattcatcg
     3301 atcaattcgt tcaatcagct accgaattat tcccagaatt gttattgcat tgggaagatt
     3361 ttggacgagg aaatgctgca gcgatcttag aaaaatacga agatcaaatc actactttca
     3421 acgacgatat ccaaggtaca ggaattgttg tcctagcagg tgtattaggc ggattgaata
     3481 tatccggtga acaattgaaa gatcaaataa ttttgacatt tggtgctggt actgctggtg
     3541 tgggtatcgc aaacatcctt ttagatgaga tgatccgtca aggtgtacct gaagaagaag
     3601 cacgccaaca tttctatcaa gtagataaac aaggtttatt gtttgaagat acagaaggat
     3661 tgactcctgg tcaaatccca ttcgctcgca aacgctcaga tttcactaat gctgatgaat
     3721 tgacaaactt agaagcagtt gtaaaagcga tccatccgac aatcatgatt ggtacttcta
     3781 cccaacctgg agcattcact gaagaaatcg tgaaagaaat ggctgctcat acaccacgtc
     3841 cgatcatcat gcctttgtct aatccaacaa aattggcaga agcaaaagca aaagatttaa
     3901 tagaatggac ggacggaaaa gcattagttg gaacaggtat ccctgctgct gatgttgaat
     3961 ataatggtgt aacttaccaa atcggtcaag caaataatgc tttgatgtac ccaggtttag
     4021 gacttggttt gattgcttca acagctacac gagtaaatgc tgaaatcatt tctcaagcta
     4081 gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg agcagctatc ttgcctccag
     4141 tagcaaaaat cactgaattt tctcaaacga ttgctgaaac tgtcgcaaaa tcagttgtcg
     4201 cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga agcagttgaa tctgctaaat
     4261 gggttcctga atataaatct ttagaagatt aagatcgtta tagtaaaaga acatacagaa
     4321 actttgtatg acgtctaaaa agaaaaaagc gactgcggca aaacagcggc cagccaactc
     4381 ctgctctttt gtcgcttttc tttggctttt ttcatatgat acagaataag aaaggatctg
     4441 taaaaatggc acaaaaaaca acaactgcaa ctgcagttga ggaaaagaaa aaagaaggtt
     4501 tttggtccac aaaaatcacc ggcgtaagtt tgccacttta tttgatcatg gtccttgtcc
     4561 tgatcattgc aatggcttta gggaaattac cgacgaatat gattggacca atcgcagttt
     4621 tggttatttt cggtaattta tttcatttta tcgggacaaa aatccctatc gtcaaaagtt
     4681 atttgggtgg tggttccgtt tttgcgatct tcgcttctgc tgcgattgct acatttggga
     4741 tcttacctga atatgtagtg aaatccacag aaaactttgt cagcaatatg ggcttttggg
     4801 acttttatat tgctgccttg attactggta gcatccttgg tatgaaccgt aatctattga
     4861 tgaaagcttc tgttcgtttt attcccgttg cacttatttc aatggtcgtc tccttttttg
     4921 ccgtaggact cgtgggcatg cttatcggaa atggttttgc aaattccgtt ttatacatct
     4981 cattacctgc gatggctggt ggtgttggag ccggagccgt tcctttatca acgatttatg
     5041 caaatgtttt aggtacggat gcttcttcta tcatttcccg cctgattcct gcgacagcaa
     5101 tgacaaatgt cttagctatc atcggtgcag cattagttgc tcgtgctgga caatcaatgc
     5161 caaaattcaa cggcaatggt aaattaatgg aaaaaggcga tcttggcgat ggaaaacctc
     5221 gaactgtgaa accttccatt ccacagttag gtgttggttt gatggtatca ttgactttct
     5281 tcatcttagg acaaattggt aactattttg ttcctaaagt ccatgcttac gcatttatga
     5341 ttattatcgt tgtcatctgt aaaatcgcca acctattacc agagtattat gaagatgcag
     5401 ctatcatgtt caataacttg attgtcaaaa acttgacagc agctgttctt gccggtatcg
     5461 gtattgcgtt gctgaatctg aatgtattag cttctgcttt gacatggcaa tttgttgtct
     5521 tatgtctgac aagtgttatc gcgatttcag ttgtttcagg atttgtcggt cgtttgtttg
     5581 gtttatatcc aattgaatca actattactg ctggcttatg caacaactcg atgggcggaa
     5641 caggaaatgt agccgtctta tctgcagcta atcggatgga acttattgct tttgctcaaa
     5701 tgggcaatcg acttggtggt gccatcgttt tgatcatctc tggtttcttg atgcaattac
     5761 tttcttagtc aaaaaaacaa aaatacctga tcatcaagta tcggcttggt gatcgggtat
     5821 ttttttgata aagatttaca ggattttcaa aaatcttttt tgcattttat cggaatgata
     5881 accgatgtgt tcataaaatt ggtgtgcttc tactctgtat tctgcagaat taagccttat
     5941 tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca gtcatcaaag cctttccgat
     6001 gcctcttttt tcagcatatt tagacacagc aagtgccata atattgaaca ttggatcgct
     6061 aaaaatcgat tcataggact ccgcttgtac atagccagct gcttttttag aagtaggatc
     6121 ttcataaacc aagaaaaaat ttttctctgg ctccttcagc agtttcttca tttgtctcac
     6181 cgtaacggtt aatggtatat cataccctaa ttgatccgca ttgatttcac gtagtgcttc
     6241 tgcatcttct aaacgcattt cccgtaacat ccaaatcacc agccaatttc tgatttttta
     6301 aaatgaattt ttttatcata gtatactctt tttttcattt agaacaatat gtttcagtga
     6361 ttttgacttg cgttagtcta gattctccta atgtttgtaa gatgacaaag gcattgtaac
     6421 aattgaccaa aagaaccacg aggacaatta cttgaaaaat aaaggtcact ttttttggag
     6481 acaaaaccct gctaaatctt gctccccaca aacctccaat aacagcagcg gggataacat
     6541 aaaacaacat agataaatca aagcgcataa agctacttgt taaagcaatc gttaccaatt
     6601 ttgccagttg agaaaagaaa atcgtagata gagaatatag tgttgcttct tttattggca
     6661 atgcaaacat cagcatcaac aaagaaacat tgactggtcc accaccaata cctaaaaaac
     6721 tagctaaaaa gcccagaacc cccccacata taaggtacca agtccatgac gtcaaatgaa
     6781 attttggctg atgatgcttg gtgtagaaga acgcaaaaac caaggtcaat accgtcaaaa
     6841 aaatttggat cagctgcaca tgcttctcgt tttcaaataa ctgtaaaaaa acttcgaata
     6901 caatgttgcc gactactcct ccaacaacag ctccactaga tacccaaccg acaatctgcc
     6961 aattcagtga tttcccgctt gatacttgtc gcacagtcga aacaatggac atcgtgaata
     7021 ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg ggcgccgata agatcaagaa
     7081 tcggtttgat caacacgcct ccacccatcc cggaaatagc gccaactgtg ttcgccagaa
     7141 cgatcaccac aaaataaatc agaccaatca tcttgtattt cctttctctt gtttatatct
     7201 tttagcgatt gttactcttt tcttttatta tacagtaaaa gaaacaaacg aggcttcgaa
     7261 agcttttcta ctttttaagg aaatttagtg ctaaacgaaa agaggttgtg aaaaagctgt
     7321 cctgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcataattt tcgtttggtt
     7381 gaacttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt cgtgagcctg
     7441 ccgttcagct ccgagtcaca aggaacaatt tcaaaaaaca gcttctcatg tttgttgaat
     7501 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttaaggaaga gactgtgaag
     7561 tcaacgtttt gacctgatta ttagagaaac aaatggcaaa atagttcttc atatttcgcc
     7621 gtttttcgag cgaatgccga ggggcagctg attgaacatc gtttatttgg aaaaagggat
     7681 tgtgacaaga cgtttgtcac aatccctttt tttatatctt attaattcat tcttttgttc
     7741 tattcttctt cttccgccat gaatgagaaa cccatatcaa agaactataa aggttatcaa
     7801 aacagcgttt atgactaaat gaattagagt tatctatcac tatttttcaa gcgatttaaa
     7861 ccaaatggca tacaatatgg catacaaaaa cttcaataag ccgtgggggt ttttaaattt
     7921 cttcttgttc tttaaaatca atgagtggag ctacataatt tgaatccact ttttcaaacg
     7981 tcactttttg catcgacgaa tgttttcttt cccaagataa cgtaaataga tttgactctg
     8041 gacttataaa gtctacttca ccttctagta ttactttctt atgcaaatat tcctctaata
     8101 aatatagact tgctaaagca tacaacacat tttccaaatt tgcttcctta taatttgaag
     8161 aacgaccatg ttttactctg ttatatattt tccaccaatc tggtgtatct tcaggccacc
     8221 catctccaaa aggttttagt gaagtcaatg ttaaagaatt cataacccta acaccattat
     8281 ccaaacacat taattcgcta gatttaataa cagctttaaa atcagttatg cttttattgc
     8341 tactgctatc aaatccgcaa agttctttga aaataatctc acactccgaa ccaatcgcta
     8401 gtagtagttt ttgatattca atagagaatg ttttgaaatt attctcatcg agcatcacaa
     8461 aatctgtggt attaataaac tcattttcta aatgtagata atacttccaa taaaaatttg
     8521 taaactctgc tttgtccatt aaatcacctc ttttgaatta taacaaaaaa aacacgtcca
     8581 caacagacgt gttctttgat ttctacacaa tcagcttgcc aaaaccatcc aatcagtcct
     8641 agcatgcctg tattaaaatt gaacgttcat catctatcat ctttcttagt ccagcatgtt
     8701 tgcaaccaca cctacaattt tcagtccaac aatagatttc gactagctaa gtcttgcaaa
     8761 attcaggcag ctaacccttc atttactacc ttaattatac taaataccca caacgtttac
     8821 aagtgtaatc attctaagaa atctgtttag ctatgatcgt tgtataatct ttaaacgccg
     8881 ttccttcatt gattgaaata attttataac tcttgttttt ccaagctact ttcatatcgt
     8941 ttgttagttc ataatcttga tcgtatcgaa taataaacgt caacgttcct tcaagaatgg
     9001 tacctacact agcaagcacg tcttttagca tttgtgaacg cacattagcc caacattcaa
     9061 atacagtttc ttcactatcc accatctgcc catcttcatc ttttacccgt tttgtctttt
     9121 taaacttgac tctctcagtc aattcattta cattcttaat gattggcata ataagcacct
     9181 ctcatttgtt gtactaatgc ttgtaccgtt actggtatcc gttcgctgct ggcttcttga
     9241 cggttcaaat accaatgttg agtaagtaat gacaccgcca aatcaaattg ctgataatta
     9301 tccatatctt tatccgttgc gctactatca atagcacttt ttatatattc gcctgctgta
     9361 tcaatcaatt gttgaataaa gtcatcatca atagtgtgat cgatccgcat actttttttt
     9421 atgctatcca aaattaccat tttatctctc ctaatgaaaa aaaggcagtg ggataaaccg
     9481 ccgcccaata tttttctata cttttggtgt tacaactggc gtaaactcaa tatacactga
     9541 agcattttga tcaattttct tataatcgtt gcgaacgatc actgcaagtc cttgtgagta
     9601 gtaatcgaat ttttcccatt gagtcgttac ttgatttcta cgagcaacaa agatagattg
     9661 tgcaatatcc cccataatca tagggaatgt tccagcttta ggattggcaa acaatgtatc
     9721 tgcaatcaat actactggca taccgaataa tgatttacca ctaggtgctg tcacatcggg
     9781 ttgtaaaatg taacgtccat ctgaatcttt caatgtatct aggtggtaat atccgctttg
     9841 gtttagaatt accattttat ttaatgctgg gtctaatgcc acattgtata gttgtttcaa
     9901 atcgtccaac gtagcagccg ttttcttagt gaatgttttt aataaatcca tgatatgctt
     9961 attgtcagtg ttttctacca atttcgctaa ctgatctttg acctcttgta caatattcac
    10021 tgctgaatct tccacaacct catttgataa ggcaatctta ccagcacgag tttctacttt
    10081 ataatcaact gaagtaaaca tttcagcgtc aatatcacca atttcagcaa gttcagcttt
    10141 tgttgctaac actgcttgtt ggttagttgc tactggatat ttaccttgtc catttgatac
    10201 tgtttttact gttgcatatt gagctaagtt ataatttgaa cgtttcaaat caaagacttc
    10261 accgattact tcttcgggaa caactgccgc tgcatttaca gtagtgactc catcacgcac
    10321 ttcgccttgt gaacggatat attcttcata cccacgcaat tctgtttttt cgttatcgat
    10381 taaagttttt tccatgttat tactctcctt ttgtgcttta gaagtggatt ctaagctatt
    10441 gtttgtttgt tttgcttggt tgttactcat aaacgattcg taagaacgct tatttacttg
    10501 gacatttgat gaatcataag cgggaacggt cacgacacta atttcattaa gtgctttgat
    10561 tttatctatt gatcgttcaa ttgtgccatc ttcttttttg tcgaatgaat ctaatccaag
    10621 cacgaaccca aaggacatag aatcaacaac cccttttgag atattctcat acacatcatt
    10681 tgaataggtc gtgtcggtta gctctgcttc aaaatgtaat cctgtatcat cgacattcaa
    10741 tttcaacgta cctgctttga cactagctag tggctttgaa taatcgtgtc catataataa
    10801 gaacacattt gataaatcca cctcttttaa tgcttcgggt gtaatcactt ctacaaagcc
    10861 gcctaaatct tttgacggtt gcccgaattt caacgcatag cctgaaatag ttttgccgtc
    10921 tgttggttgt ttttcatttt ctttctctac tttgggagaa tcggctgtta gctcagcttc
    10981 ttctgtcagc cgtttttcct tttcttcttc attattcaac tggtgtaact ccttcctgtt
    11041 cttttaattg atatttctcc aacgtatcta aatacgtaaa gttcaaactt gctagtaaac
    11101 gatcgccacc atccatgggg ggtagcccca ttttcgaccg cccctcatta atcgtgagaa
    11161 gtgacccttt gacctgatcc aatacatttt ttacttttgt ttcaggatca gtctctagta
    11221 actgatcgga attaaagcgg atattggttt caagtttcgt atctaattca ctcacaaaca
    11281 cattaaagta atgaatcagc gtactttgga tatactggag attactttga acggtgctag
    11341 aatgttcatt ttcaacgcct aaacgctcaa tcggtacacc aaacgccttg gcaatttgtt
    11401 ttgtgttcca atcattggaa ttgataagct tcaaaacatc ggtgtttatt tctaatgttt
    11461 tgtagtccat tgtttcatca agaatgatcg ttctaagagc attatctcca ctacttgaac
    11521 cgttggcttc ttcaaacttt tcacgaattg cgttctttgc tgatccgtct aaatctgact
    11581 tatgaacctt caaaatacct gacccactga caccacgagt aaagaaattg tgcaatgtgc
    11641 ggttgccagc ttgttgaatt ttcagttcat cacgtaacgc ataaagtggt ggtaatcctg
    11701 ttaaaccatc ttgggtgaaa tatttgaagt gcaaaatatc gctagacttc acacgtcttt
    11761 ttttatcacc aatctgataa gacaacgtac cattatctaa ttgagtaacc gttacacttg
    11821 agttaggtaa taagtgaatt tcttctacct tttcaccgtt gcgtttaatc tctgcaaagc
    11881 tattaccgtt tagcaacata ttgaccgcca aagcaaactt taaatgccaa ccatccattt
    11941 ctgaattagg tttctcgttt agtaacttca ccagttcatt atcagcttgt ggcatattgt
    12001 ttttgaccaa ttgaatcgga cttgaagcaa tatcactagc aataattcga acggctgcaa
    12061 acacatcact atttcttaat gcacgaacgc ttgtaaagct ggtggtgtaa tcatccgatt
    12121 ggattgatac cacatgttgt aggaaaggat ctcccgttgt attttgtctc gctttaaaaa
    12181 atgccatctt ttaacctccc ttctgttctg tatttagcat gaccgctacc ccaataagca
    12241 ctatgcccag cgctaacaag ccatagtaga catttgttag taaggtgata gcaaccaaca
    12301 cacacaccaa tccaagaagt aaaagaacgg tatgaatgta attagaaaga aaattcttca
    12361 cttgtataaa attcattgtc tgctttcgct ccttccgttg tggtgaagta atccattgca
    12421 aacacatagg cattgattaa tgcagctatt ggatcaattt tgttactgtt ctttgattta
    12481 ttaatctgaa tgccgttgtt atccgttttt aaaatagcgt tgttcactgc atgggtgaga
    12541 atcgtatttt tattgtgaat aacgttgcct tcataaactt gttctctaaa agttcgggtc
    12601 ggaacgttta gagtaatcgt tccttgtctt acttctaaca ttgggtagtt ggctttttca
    12661 gctttactga taagcgaatt agcgttatac gggtcataac aaatccccat acattccaaa
    12721 tcattttctt gaatcaaatc ttgaataaac tgaaatactt ggtcatagtc cacgattcca
    12781 ctttctaatt gagtaatgga acactcacca gctttttcta gttctcgata aggcaaaccg
    12841 tcgcgctttt ctttgtcttg aagcccatat ttggtggcta caaagctatg tgaatcacaa
    12901 taaagttcgc cgttatctag cggtacgatc catgaaacgc tagttaaatc atctgtttta
    12961 gataaatcca ctccgatata aacgggcttg cctgtaatat cttgtggttc tacttcggtt
    13021 gcctgccagt cttcagcaat catatagctg tcctcactcg cttgtctcca taaattgaag
    13081 tttttcacca atacagaatt aaggttattt tgtttcaaag caaggttcac atcatcttga
    13141 atggttggaa tcattacttt tttgatttct tcactttcaa aaatcggatt ggctttgatc
    13201 catttctctt gatcgtgaat ttcttcggga tcgtccaatt cccatatcgc tataaagtac
    13261 ctgtcggctt gttcttcgcc ttttagcaca cgatctaaca ataagtattc ctcgtacata
    13321 ggaacgttta aatcaagccc agcggtacta ataacacaca ataagccgtt cttttgttgg
    13381 gtcattcctg acttaatgac gttgtacgtt ttacgagttt ttgcttcgtg ccactcgtca
    13441 agaattgcgg ttgttggtgc aaacccatct agcgtgctgg tttcactagc cagtgccata
    13501 gcgaacgaat tagagggcaa gtgctgaatc ttagagttca taattttcag ttgtggtctt
    13561 aaaaacttac tagacttgac cacgtttcta agtgaattag acagcatatc atagcccaat
    13621 ttagcttgtt tcaaagcgtt acttacaaac aatacttgcc ttgcttctgc tggttcttgt
    13681 tccataatca acgcattggc agccatgcca cttgcgatat acgttttccc attcttacgg
    13741 ctcatactgg taaacgctcg attgaatcgc ctgtaattcc cttctttagt acgccagcca
    13801 taaagactgc ctagaatgaa tttttgaaat cctaacatct tgattgcttt accgtccgta
    13861 gatggtagca actcaatgaa tttaatcgct ttggtggctt gttcttcatc gaacacataa
    13921 gggaagctat ctgacttaga acgctccaaa tcgcttatat gacgctcaca cgcttggata
    13981 attttcttgg gtgcaatcag cttacctgat aggactttct caatgtaatc attcatgaat
    14041 tcatcaactc tttgaacggg tcttcgggtt catcattttt aaccttgttc aaagctagtt
    14101 ttgatcgtga ttctaaggtc atacctagtg aggtggcttg ttttttcaaa tcactcatag
    14161 cttgtgattg aacccgaacc gctggattgg ctttctttac tccgttttcg catgtgataa
    14221 acgtacctga ttttcttatc tcggcttcgg ctgttttcat tcgagcaaag gctaaacaat
    14281 aggctaccaa tgaaccataa tctgcttcac tcaatggata atcttttttt aatgttggta
    14341 cgatacgatt ccattcactt ctagcgctcg ctgccatcca gtcagggggc gtgatattta
    14401 ttaatggttg ctgcttgaac aattcttctc tagcgtccac acggattgct ttttcttcac
    14461 ttgatatatt gccttttgta tcgtgtaata ttttagggtg tcccatgttg tcctcctttc
    14521 agacaaaaga aaaaggaacg taagcaatta agctcacatt ccttcgtttt tcgatcagaa
    14581 tttttcttta tctaatttat ctatataaat gatttttccg ttctgcattt gaatggtcac
    14641 ttgaccaaaa tgaggtaact caatggtgcg ctgtttccct gttttatcag taataacaat
    14701 tgtatttttc aataaatctc ctactttcat tatatacttt ttcacttgtg attacaagtg
    14761 taatcgttat ttgtaattac ccttatgcct tttcacaacg gttatttgta ccaaaatgag
    14821 taggcatcga ttgtcagtaa gtttcacact atgccccctt aactcaaagg gtactctttt
    14881 ctcgtctttg tctttcattg gctgtcttgg aattatggtg tgcatggcat aaagaacgaa
    14941 aattagtttc atctaaccgt ctattccaat catcttttag ttcaattttg tgatctacaa
    15001 catcagcttt ccttatcaag ccttcttcta ggcaatcctc acatactgga tgatttaacc
    15061 gatacaagta tgaagccttt cgccatgttc tactttgata gaattgaaaa tatttacctt
    15121 cgctttgctt tctttcttca cgcttaattg ccgtttgctt tgctttatgc ttcccacaat
    15181 acttctgcct ataatcaatc aatgttttac atccagcatg attacattgt tttttaactg
    15241 ccatagatta ccaaccaaag cgatctcgtt cgtcagaatc atcttgttcc cattttgatt
    15301 tgtattcaat ataaggcgaa actatcttca agcacgttcc gtctttaaaa ttaactttag
    15361 tgattttatc ttcctcaaaa ctaataaagt ctacatcttc ttgagtgttt gaatactcaa
    15421 tgtgtttctc accatcgaag tatctaatac tttccatatg cataattatt ctacctccaa
    15481 taaattagtt atcaattcat catctgttag tagcaaagcc aagaacttat catggatgtt
    15541 taaaaacaaa ttatcctcat tcgatgtaag ctctcgttgt ctcgctcgat catataggtt
    15601 aagcagacaa ttaaattcat ccattagctc acctgtatca tcatagttgc cactatctac
    15661 caatgatccc actactagcg attgacacca cgctactgac tcctgcagcc aatcaggttc
    15721 ttcttccatt atctttaata cttctggatc aaatgtggaa taatcttctg ttaccaatcc
    15781 cacactaaat gccagcgtgt tcattagaaa ccgatccatt tcatctgtat attgaaagtt
    15841 cggtgctttg tttgttgaaa taatattgag catttacttt cctccttgat tcgtaacacg
    15901 tggtaacacc tacgtaacag gtacccgtta ccactcaatc ccaacgggga caagggttct
    15961 agcgttttgg taacaggtaa cgggtaaaat ccagtaacat tattccccag catattagct
    16021 aactatttca aaagttttaa ggaaataagg tgttaccccg ttaccaaatt gctgtatccc
    16081 ttggggcagt aagggtaaac tagtaacggg ctaggtgtta ctgacccgtt actacccgtt
    16141 actttttgta ctttccagct cgatttttat tttcataggc atttgactta ttgtaaaatc
    16201 ctcgtcgttt atttcttttt ggcagttgct tccgttccca accaggaata ctgctaataa
    16261 tcaaggcaat ctttttagtt tctgcatctg atcgaccatt caataaatcc tttgcttgtt
    16321 tatcaaaaac agcttccaaa atatcagcgg tgagtacgct atcaactaaa taaaactctt
    16381 gttgcatagt agctccatta tagaaatgct gtaaattttt ctcattacat ttactgtcag
    16441 gatagttctg ataataagaa cgtctcgccc acgctggagc ttcttcccat ttatacggga
    16501 tttccatttc caaatattta gtgattgctt ctttaattgg attctccatc ttggcatcct
    16561 cttgatagtc ttttgcaatt tctaagactt ctttatcttc atcgggacta aaatagattc
    16621 gttgtccttt atcaaaaagc acctttgctt cagctaacac ttgaagaaaa tattcatcct
    16681 ttgttttaaa cggattcatc tttggcttat tcttacaagg caaaggataa aaacggcggt
    16741 ttcctgtatc atctttcaaa tactgcccat cattcgacgt tccaataaat acacattgcc
    16801 ttgcccaagc tatgacattc cgaccataag gaggtctaat tttatcgatc ttggcactga
    16861 tgaagttttt tatctgttca attttagttt tgttcattga agataattct ccaagttcaa
    16921 taattacatt gccttgaagc tgcatataat catctttctt ttcacctagc gaatctaatg
    16981 aatcggtgaa aaaatcatcc gtgtaaagta atgctggaac ggtactctta ccaatccctt
    17041 gcggtccatc caaaactgga acgatctcaa actttattcc tggtttatag atacgtgcga
    17101 ctgatccgac tatccaacgt tttgttactt ctcgtgtgta aaggttgtcg tctaccccta
    17161 aaaggtcaat aaacagtgtt tccagtctag gctgttgatc ccatttaact ttttcgatac
    17221 gttcttttac tgggttgaaa acattctttc tagccacatt tactactgct ttgtgtattt
    17281 tgttgtcatc aaaaactaca ttgtagtttt cttcaatata tgccgttaag gcatcctcaa
    17341 aactgtcttc ccattctcca gcggtacgat tgatccaagg agaatctttt aataggtgaa
    17401 tgtatccgct aaattcgtta taagcaattg ttccagctaa attgctatca ttggttaaaa
    17461 tcaattctgc attcacaaca ctgttacttt taatctgatc gtctttattg catcttaatc
    17521 gtgacatcca ggttttcaat tcaatgatat tgtcttcacg ctcgctggct tcgggaaagt
    17581 ctaaaatact atctaattta tgcttatgag ccatgagctt tccccctcaa atctttttta
    17641 atcatcgatc gaataatttg attcacttct ttatctttta gcggtggttc attgtacgca
    17701 tttgctgtat aaatcaattc tgctaaagtt tgattatcta caccgtgcca tagtaaccaa
    17761 ccgcataact tagttaaatg atcgtttcta ctaccttcgc cagcgccttg aacaatcata
    17821 tctaattttt ttgctgtccc tgtttttgct acgggtcggt attgagtctt tatttcttgc
    17881 ggttttttta gcagtaggtt cactagccaa tcgggagctg gtgcaatatt atttagagat
    17941 acgttttcaa taggttcata tgctctccca acacttcttg aaataatcat agttggcgct
    18001 attggaactc ctttagtgat aatatcaatc ccgcttaaac ttttaccttt atcatcacgc
    18061 ccgaagatat cagcaaatgc acctgtttct tgttggataa ttacaccatc aggaatttta
    18121 aagaagaaat ggattccttt acctggtgtg atttccgtat aagtagaagg aaatggatca
    18181 tacattgaag caagtttttt ataattctca acgccgtttc gtgtttcaga atgttgatct
    18241 aggtcaacta ccaccatttg atgatcttca agcattaacc caatatttct agttgcatct
    18301 tccgtccacc attcattaat ttgattagaa tcgttagttg catcattcca accactagag
    18361 ccttttattg gaattttagt tccaggagat aagggataaa ttgaccatcc cgacttttgt
    18421 aattccgttg ctgcttgata attatttgcc aataaacctc tcctcctttt tcaaaatact
    18481 ggtataaatc attcagacac ctccatgagc ttcaactgct caagttcgta tttcaaccca
    18541 tcccgaacaa attcagcacc ttttaattga ttctgatact ttacccatgt attaaatgtt
    18601 ttcacccttg cttcttgcat atcagtaatg tttaaccatg cggttaaaat ctgatttttt
    18661 agctcgttga tttctcgaca aattattttg tgttctgcat gttttttgag tacatacaaa
    18721 actgttagtt tttcattgaa tgaatttcca aagtaggttt tcccgatatt attttctagt
    18781 tcagcgatcc cttgattaat ttcctctaat tcaaggctat cgagttggtt taatagtttt
    18841 gttttgtaca tctacttatg ccctccagtt cttaattgat agtcgtactc gtcataagca
    18901 gtgaagagat ataacgctaa tcctgcaaag atagctattt ttgcaattaa tggtaataag
    18961 ccgaaaattc cgccgattaa aatgcttcct ataattaagt ttcgtttcat aatggttccc
    19021 tccgattttt taatatggta taatcggaac aaagaatatc gggttttatc ccaattaagt
    19081 tgcctttatc ctgtccgcca agactgttat ggataaggct ttttctttgt tctcgatcat
    19141 gttaatgctc ctaagttgtc tgcaattcag cagaacgatt ataaattttc tcactcactg
    19201 aaaactgcaa cccatatttt tctttaacag tcattaattc tactgtctca tctaaaatag
    19261 gttcacgatc aaccaacatt tcaggagtca tatctttctt ttttaccatt ttggaatgac
    19321 cataaatagt tgatacagct ttattggcta tcacattcgc tttaatgtag tctactggtt
    19381 ttggttcagt aatagcgtgg cttaatttag tcattgcttc tttttggtgt tctttgtcta
    19441 gcatgcggaa tacttgaaag ccttcaagtc ctgtggactg gcgtagttct ttgatgatgt
    19501 cgaaaatcca gtcttgaaat gcctcggctt cttttttacg gcttttgaat gccatgcggt
    19561 aaatattttt tttcgttgat gatgtttaca tcttgtgttc tgcccaatga atcgatgacc
    19621 ttactagtag taaccccatc tttaggcaaa gagtgaatgg ctctagtaac ctgtttaaga
    19681 cctaacgctt ccgcaacatc tttcgccact gcccaccatt catcattaat atcgacaaat
    19741 cttataatgt gtccgttcca aatttcagtt ttcattcaac gtcctccagt tcaagcccta
    19801 gcaagctcgc taacaactca acttgctgat acaaaatttc ttgatactct tcaatagaaa
    19861 gtcgttcttt aaactctgct tgtactaaat cgttcaagcc acgcaatact ccgcttaccg
    19921 ttggttcttg tggtagcaat ttttgatagt ccatttgttc gtacaaacat tcaaacgttt
    19981 cttctagcgc tttgccttcc aagtttgcta attctgccgc tgtgtcttct gtaagaattg
    20041 ttgataatac tttttcgatt gctttatttt gtgtcgattc cattattatt tcctcctatt
    20101 aatccctttg cccccacggc ttgcgtttat ttttggtatt cttccataaa gcgatcacat
    20161 tcatcttgtg aaattcttac gactccatca actataatca actttaaacc gttaggaaca
    20221 tatttctgca tcatagtatt gtatgaaacg ttcatatact tagcagcttg tgccttgttc
    20281 atatattttg gaaactggta ctttttttct aataatgaaa gtaacgttcc ttgagtttgc
    20341 tctacttgtt gaacaactcg atcgtcaata attttaaaag ctggatcatc aagcgtgagt
    20401 ttgtcaattt ttaattccat atttatcccc cctcgttagc atacatttct atacaaagat
    20461 tttaaaaaaa tgcaagaggc tcttgaatac atttctatac ctcttataaa ctcaatatac
    20521 catcatacct tttcatttgc aagaatttta acatactttt ttgaactttt atgtttcaca
    20581 catatttaaa tcgtgatata gtagagaaaa tatatgaatg gtggtgaaaa tgtggctaga
    20641 aaaaaatacc ccgaagcagt ggagacagga caaagaatta gactggctag aattgaaaag
    20701 ggactctcaa tggaacaact tggaggtaaa ttatccccac cggccagtaa aggtgccgtt
    20761 agtaattggg aaaatggcta taatcttccc aataacacca ggcttaaaca actttcaaag
    20821 gtattagatg tatccactac ctatctatta aacggatctt atacattaag agatatgcat
    20881 atgatgcccg aaattaagcg aaatgtgctt gaaaagcaat tgaaaaaaac tattgaaaaa
    20941 aataactcta taattgattc taatctttct aatgctatca ataacatcga agaagataac
    21001 ctgactgtct cacagaagat ttatttaaca aacgcaataa aatatcttaa aacaggatct
    21061 cgaattgaat ttcctgacgg gagtacccca attgcttatc tcgcagctct tctaaatacg
    21121 cttttacgat atgactcatt aattaatgat tcagaaaaaa atacagatga gaaaaaagag
    21181 tatcaagaat tttatgaaaa tgatcttcta aaagagttca cagctcttgt tactgagcta
    21241 aataaacact attcaaaata ataataaact gcctcctcca accgccccca cggcttgcta
    21301 cactatggag ggaaaactat tatggcaaca ttcaagcaat atgaaactaa aaaaggtaaa
    21361 ttttggctct atgaagccta tcttggaatg aacaaaatga ctggcaaacc tgataaggtt
    21421 cgtagacgag gttacaaaac aaaaaaagaa gcccagcttg ccttgtcacg cttgcaagtg
    21481 gactacgata aaaacggtat taaaaaagcg aacaacgaaa cgtttaaaga agtttatgac
    21541 ttgtggctag aaacatataa aaccactgtc cgagaagcca ccttcatgaa aactgaaatc
    21601 aagtttagaa agtggattct tccaaaatat ggaaatcttc gtgtgaatga agtcacggta
    21661 aaaaaagccc aacaaattgt aaatagctgg gctgaaacta ctgaccaata caaggtactt
    21721 cattcaactt ctaagcgtat ttttagctac gctatcaatc ttgggattat tgagactaat
    21781 ccactagaca acgtattaat gcctaaaaga accaccacta agcataaaga aaaggtcaaa
    21841 gtttacagta aagaacaact gatcaccttg tttgattacc taaacagcaa acctgaaact
    21901 tatcgaaacg actttgataa aacgttacta cgctttctct tttatagcgg ggttaggatc
    21961 agtgaagccc ttgcattgaa ttggtcagac attgattttc aagaaaatac ggtcaccatc
    22021 aataaaacac taagccaatc caaaaatggc tataaaatca gtgatcctaa aactgattgt
    22081 agtgctgggg ttcttccact agatgaaaga acaatttcta tattaaaaaa atggcagatt
    22141 aatcagcgga aatatatgct gacacttggt gtaactgatc cgactatgat tttttgtggt
    22201 atctataaag aactaatcac acaccacgct atttatgcaa gattgattac tatttgtgaa
    22261 aaagctatgg ttccatttct cggtgtgcat gtagcaagac acactcatgc ttcaatgctc
    22321 ttggacgctg gagctactat ggtagaagtt caaactagat tacggcatac aaaaattagt
    22381 cagacgatcg attgttacgg tcacttggca aaagaaacga aagaaaagac tgttgaaaaa
    22441 ttagtacaac acttaaatat tgactagatg gcatacaata tggcatacag taaaaaataa
    22501 aaaaaggaga aaccctatca tatcaaggtt tctcctagct ttatagtcta ttcttcttct
    22561 tccgccatga atgtattgaa tacttcttca atcatgtccc attctgcgtc tgtttcgatt
    22621 tgttctaact cgccttctgt tccatcttcg ttttcgatgt atgcataagc ttgtaattct
    22681 acatcttcat cttctgaaac accagcagga tataaaagaa catagttacg tccaaattct
    22741 tcttgtccgt caactgttag taggatttca tataatgttt catttccttg gtcatccacc
    22801 aatgtgatgt gttcgtgtcc ttcatggtcg tgggaatggt tatgttctgt cataagatta
    22861 cctcttttct tttattatta aaataattgc ttacctgagc cgtctaaata attttgtaag
    22921 atcatcacag ctgctaattt atcgatgact ttttttcgtt tggctcttga agtatttgcc
    22981 tgctctacta gcatacgttc tgcctgaaca gtggttaatc gttcatcttg ataatcaact
    23041 ggcaaggaga atagttcttt gatcttttct ccgtatgaca tggaagcttc tgcccttggt
    23101 ccgatggaat tgttcatatt tttaggtaat ccaactacaa accggtctac ttcatattgt
    23161 gctactaatt ccttcaaccg atcaaagcca aactcttctt ctgcttcatt gatccgaatg
    23221 atctctactc cttgagctgt ccagccaaag gggtcactga tagcaacacc tactgtctta
    23281 gaaccgacat ccaagcccat tactctcata aattcactcc atgatttgag aggtagtcct
    23341 tgacaattac ttccatgatc tcatcgcgtt cgtgacggcg aatcaaattt cgagcatcac
    23401 gataacgagg aatgtaggcc gggtctccag aaagcaagta gccgacaatc tgattgattg
    23461 gattgtagcc tttttcttcc aaagcccgat aaacaatcgc taatgtttcg ctcacttctt
    23521 ttttgcgact atcgtcaaaa ccaaaacgta cggtttcatc tgtaaaaccc atgattctac
    23581 acctctttcc cgcaaggtaa ttccagctta ccttctacaa ccttatttta ctcgaagaaa
    23641 gggaaaacta caaacaaata ttccaaactt ccgaataaat taagaaacac tatttgtatg
    23701 tttttttcct tttttctgta gctttatcaa aagtactctt ttcataaagt atacgctata
    23761 tttctttgaa tgtatgtttt tacacaattt tttcataata ttcataaaaa attcgaatag
    23821 ctgctactaa ttttttgttt ttgttgcttg tcctgtctca taattgatcg tgtagccatc
    23881 ttgaacattg tagataaaga cattgaattc aatttttttg tcttcaatgc tttctgcttc
    23941 caattgcact cccctggcta ccagttcatt cccctcaaaa tgaggagtga cacggtagcg
    24001 tacatgatga tttgtttctt tgatatatgc cgcaatatca ttttcgtgag caagcatgta
    24061 tggagcattc aacgaacgtg tccctgtcat caagttttta atgttgttat tttgaccagt
    24121 cagctggaaa ccaattaaat ggctgcgatt atataaccat tgcccgtcac ttaatttctt
    24181 ttgtttccaa cctgtcgggt caatatacaa aggctctctc ttttcagtag ggaacaaatc
    24241 ttttccaagc atagcattgg cttgtcctac tctgttcaat ttatcaatat ttgaatagct
    24301 ttcccatgtt cctttggaaa ggctaagatc ctcttttgta aattccggct gattcccatt
    24361 gatttctatt tcttgatgtt ttccatcata ttctggcaca tccgctactt gagaagcttg
    24421 tggtgtggaa tcaaaaatag ttgtaatctg ctgaaagata tctgaatctt tttgtgtggt
    24481 ggaaacaccg aatgcccctg caatcacagc agcgacgatc cccacgatcg ccttggttgt
    24541 ttttttgctc atttctttac tcctttacta acagctccct aaatcttaac atatgttccc
    24601 ctataaataa atactagaaa gaaatagaaa cagcaaaaaa taaaacctgt tttaagatgg
    24661 cttaaaaata caaaaaagag acggattcct cgtctctaat cagtcaccac tctatctgta
    24721 aggtatttat tattattcaa aaattttctt catactcaag acaatacact gatttcacaa
    24781 ccgcttggta aacggtgcgc aaaagcttgc atctgcggta ataagtccat ccaagttaaa
    24841 aacataagaa gttgtttttg cttggctgtt cttattactt gttgcagagc agctttttca
    24901 caacctattt taagcgggtg acgggaatcg aacccgcgac atcagcttgg gaagctggga
    24961 ttttaccact aaactacacc cgcaatacaa aaacagtata cgcctctttt attccttcct
    25021 caagtttttt tattctttac gcttctggta tgttttgtag taaaataaaa ctaatcaatc
    25081 aattattgga gaagatgcta tgacgggaaa agaattagat ctgttgctgg acacttgttt
    25141 actcgctggg aaaatcatga tggaaagcaa tgcggagatg tatcgggtag aagatacgat
    25201 gagtcgaatt gctttagctt ctggaaattt ccgtttggtc agctatgtca ctcaaaccgg
    25261 tctctttatt ggtcttgatc gtacctcgac catccgaatg gaacaaatta ccaaccgttc
    25321 gattaattta gaaaaagtag taaaagtaaa tgatctttca cgtaaatatg ttgcttcaga
    25381 gctaacatta gaagaacttt actgcgaact gcaagcaatc gaccgagacc gttctttctt
    25441 tcctgtctgg ttgcaaatcg ttagcgcggc agtgatcagt ggaacaatta tggtgttatt
    25501 tggcggaaag atgaccgatt tacctgtgac tttagtcatt ggaggattgg gatacgcagc
    25561 ttatctatat agtttgaaat tttttagaat caaattttta tcggaatttt tatcttcttt
    25621 attgattggc atagccgcta tctacagcgt acgttttggc ttaggaagca atcaggatct
    25681 aatcattatc ggatgtgtga tgcctttagt tcctggagtt caaattacga acgctatccg
    25741 tgatttgcta gctggacatt atgtctcagg agtttctcgc ggaacagaag cgatgatgac
    25801 tgctacgatg atcggttttg cgattgcttt tattttccag cttttttatt aggaaaggaa
    25861 cacaagtatg gggacgatat ttattcagtt ttcttttagt tttttagcga ctgcggcatt
    25921 tgccgttatt acaaatgtac ctaggcgttc attgatttgt tgtggtcttt ctggtgcttt
    25981 tggctggatg atctactgga tctgtgttca aataggcgga tctgctgctt ttggttcatt
    26041 gcttggttct ttaggagtag cattcatcag cgatcttttc tctaaacgtt tgaaaatgcc
    26101 cgtaacgatt ttcaatattc ccgggatggt tcctctagtc cctggtggtt tagcttatca
    26161 agcggttcga aatctcgtta ctgaatccta tcgagaagcg gctcattatg cggtccaagc
    26221 aattatgatt gctggagcaa tcgctttagg tcttgtatta tcagaagtct ttaaccataa
    26281 catccgtaat ttcaaagtaa agcgtgacgc aatcatgatg aaacgaaaaa aacggctttt
    26341 taaataaacg aagtgtaaca aagaaaaggt tgtgaaagag acgttcagcc ttgagcgatg
    26401 acaaaatatc tcacaaacat tgcgacaagt aaccgcagga gcaataagaa gtaattatga
    26461 gtattaaaaa aagttttgta aaaatagctt ctcacatttt tacataaaat tgacttaata
    26521 ctgagaaact gacttttgaa caccctctat ctgtaaaaaa ggggctgcga catgattttt
    26581 gtcacggccc ctcttcttta ttcaaatttt ttccaaaacg ttcgttgata ataaacgatc
    26641 catagagcgg aaagtattag aactaaaccg atcacaggaa tattcactaa ccaaaatgga
    26701 taaaacaaag ccaacattcc gtataagacg ataagaataa tactgatgat caatgaaccc
    26761 atcatagttg cgactaaacc caaacttcct gaacctctgt taaataattg gttaatattg
    26821 gtccagttca gcagcagcaa ccgataatct ctagcgaaat aacgtagtga caataagtag
    26881 cagccgaata aagaaccaaa tagcaagcta gggataaaaa acaaaggcag ctgaaaagac
    26941 agtccgctta ataatgcgat cccaccagtt aaaaaactct gcaatatcca tccgaatctg
    27001 aacttttctt ttaaatattg gttcatagag attggcaaag aacggataaa cagaaaattt
    27061 tcttgatcca aagaaataag attgctgata aaagaagtct gattaactgt taagaaagcc
    27121 aatgcgatcc ctaccaggaa aacaacaccg attaacttat tatctaaatc agttaatttt
    27181 atttcgcctc ctagggcaaa agcaagaata aaaatgattg gagttaacaa ggaagaagaa
    27241 agaacttgca taatcaaatt aggttctttt actaattgag cattgtaact aaataaaagc
    27301 tgatgcacat tttggttctt tttatgggtt ctttttattt tgaactcact gtttgaatca
    27361 gtcagttgct ctgtaagctt tggtactatc agctgtttta cagcaaggaa aaatcccaga
    27421 gccgtgatag ctagtattcc ccaacccaat aaaccttgtt gagaaagtgg ctgactggca
    27481 gcatcataga atggcaataa tatcgaaatc ggatgccgat ctgttaactc attttcaata
    27541 gtccccgttt gatgattcat ccacatgatt ccagcaaatg cgatcagcat cgagctgcca
    27601 agcaagatac tagaaactgt ttttctatgt tttttaaaca ctgctgttcg agccaaacca
    27661 aaaacaatca aactagccag acaaaaaaga ataatcaaaa atagagaaaa caaaaggatt
    27721 gagataatga tagtaagcaa aatccctaga tcagctcgcc atccggttaa aataaacatg
    27781 actagaagcg gaaaaacaaa aggaatgatg gtcaaagcaa ctaccaaaat ctttgctgaa
    27841 aaaacctgtt cttgtccaaa aggcagaggc aaaaatactt gcaggtcatt tccttcaaaa
    27901 aagatattat aaatgactga tatactttga gttaaaccta acataccaaa aagtgccacg
    27961 taataagtaa agaaacctgg caatttaaca aagttcatag caaacatagc aaatccgtaa
    28021 atcagtaaaa aaatcagccc tgaaaaaaga ttttgagaca aaagatagat agttaatcgc
    28081 tttccctttt ttcctttctc tcttgcttta ttcgtgagct gtggattaac atataaaaga
    28141 ttaactcgaa ttaattctaa taattgtcgc ttatacatac ggtccatctc ctgtcgcttg
    28201 ccttccagcc attttcaaat aaatcttttc tagcgtttca tcaggtgagc cattcaataa
    28261 ttcactaact gccccctgat aaatcaattc acctttcttc aaaatagcca gtttatcgca
    28321 taactgttgt gcggtatcca atacatgtgt agaaaaaatg actgtttttc cttttgcggc
    28381 atgagccttc atcatttctt tcaaatcaaa tgctgcttga ggatcaagtc cttgcaaagg
    28441 ttcatccaat acccaaatat ctggatctgg cagcaacgtg ccgatcaaaa tcgttttttg
    28501 acgcattcca tgtgaaaaac ttgcgattgt ttcattttga tgactataca tatcaaaaag
    28561 cgctgtcaat tctgctaaac gcttttcttt tttttgtgtg tctaattcat aggcagctga
    28621 aatcaagtcc caatattctc cagccgtcaa ttgtaaaaaa atatctgggg aatcaggcac
    28681 ataaccgatt ttttgtttga tactcaatcg attttccgtc aatttcattc catctactaa
    28741 aatcgttccc gatgatggtt caataatact aactaaactt ttaatcgtag ttgattttcc
    28801 ggcaccatta tgtcctaaaa atccaaaaat ttccccttgt cttatcgaga gagacaagtc
    28861 ttttaatgct tgttttttcc cgtaactttt acttacattg atgaattcaa tcatttctgt
    28921 actccttttg tttgttatta ctttttattg tacccttcgt tattggtgga gtaaatgtca
    28981 aaaagagtgc ataacaaaaa aggatagaaa cgactaggct gctacttggg ttgttctctt
    29041 ctacaggaaa acactcttct aacaggtaag atatgagtct ttgttttctt taaaatgatt
    29101 cattttctat cttttcttgc ataaaattct ttagtttatc aatacttctc tttcccatct
    29161 ctagttgttc tccatttgca aatgttacta tttcgtttac tctgctaata gatgtaatgt
    29221 tttcagtatt aatgatataa gcttttaaat ctttacataa atactcatat tcgagtgatt
    29281 ttttatattt tttaattgtg ccgtttacga gaatctcttc atctttcaat actaggccta
    29341 ttttattttt ataacctggc atgctcatta gataaaggat ttcatcgtaa tggatatagt
    29401 gatcgctatt accataacgg acagttgtcc accttttttt cgtagcatgt tgaacaggat
    29461 agttttcttt ttctttcaat aagtaacttt ggatgctttt tttatttttt tcataagtga
    29521 attcttttat caaataacca ctaggcgtaa tctttcgatt aataacagtt attgctttat
    29581 tcggatgaga cgttagataa acaattttac acttttcatt cttttctcta agcatttcgc
    29641 ctagttcgat accgctataa taggcgttca aatcgatgtc taggaa
//
LOCUS       DAZKCZ010000026        29110 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000026 DAZKCZ010000000
VERSION     DAZKCZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.026"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1030)
                     /locus_tag="ACG8KN_001047"
     CDS             complement(<1..1030)
                     /locus_tag="ACG8KN_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9188119.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="ACG8KN_001048"
     CDS             complement(1112..1801)
                     /locus_tag="ACG8KN_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9188120.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ACG8KN_001049"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ACG8KN_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9188121.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="ACG8KN_001050"
     CDS             complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="ACG8KN_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9188122.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ACG8KN_001051"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ACG8KN_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9188123.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ACG8KN_001052"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ACG8KN_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9188124.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="ACG8KN_001053"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="ACG8KN_001053"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFP9188125.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="ACG8KN_001054"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="ACG8KN_001054"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFP9188126.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7543)
                     /locus_tag="ACG8KN_001055"
                     /pseudo
     CDS             complement(6443..7543)
                     /locus_tag="ACG8KN_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7635..8522)
                     /locus_tag="ACG8KN_001056"
     CDS             complement(7635..8522)
                     /locus_tag="ACG8KN_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9188127.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ACG8KN_001057"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ACG8KN_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9188128.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ACG8KN_001058"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ACG8KN_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9188129.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ACG8KN_001059"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ACG8KN_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9188130.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /gene="cbpA"
                     /locus_tag="ACG8KN_001060"
     CDS             9753..10397
                     /gene="cbpA"
                     /locus_tag="ACG8KN_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFP9188131.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="ACG8KN_001061"
     CDS             complement(10453..10956)
                     /locus_tag="ACG8KN_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9188132.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="ACG8KN_001062"
     CDS             11553..12518
                     /locus_tag="ACG8KN_001062"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9188133.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="ACG8KN_001063"
     CDS             complement(12617..13789)
                     /locus_tag="ACG8KN_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188134.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="ACG8KN_001064"
     CDS             complement(13776..14921)
                     /locus_tag="ACG8KN_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188135.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="ACG8KN_001065"
     CDS             complement(14935..15867)
                     /locus_tag="ACG8KN_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188136.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="ACG8KN_001066"
     CDS             15984..17063
                     /locus_tag="ACG8KN_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9188137.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="ACG8KN_001067"
     CDS             17060..17689
                     /locus_tag="ACG8KN_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9188138.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="ACG8KN_001068"
     CDS             complement(17799..18026)
                     /locus_tag="ACG8KN_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9188139.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="ACG8KN_001069"
     CDS             complement(18129..20717)
                     /locus_tag="ACG8KN_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFP9188140.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="ACG8KN_001070"
     CDS             complement(20737..21396)
                     /locus_tag="ACG8KN_001070"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9188141.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ACG8KN_001071"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ACG8KN_001071"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9188142.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="ACG8KN_001072"
     CDS             complement(22169..23035)
                     /locus_tag="ACG8KN_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9188143.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="ACG8KN_001073"
     CDS             23144..23785
                     /locus_tag="ACG8KN_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9188144.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ACG8KN_001074"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ACG8KN_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFP9188145.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="ACG8KN_001075"
     CDS             complement(25226..26125)
                     /locus_tag="ACG8KN_001075"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9188146.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="ACG8KN_001076"
     CDS             complement(26127..26924)
                     /locus_tag="ACG8KN_001076"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFP9188147.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="ACG8KN_001077"
     CDS             complement(26902..27606)
                     /locus_tag="ACG8KN_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFP9188148.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="ACG8KN_001078"
     CDS             27737..28312
                     /locus_tag="ACG8KN_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFP9188149.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="ACG8KN_001079"
     CDS             28430..29107
                     /locus_tag="ACG8KN_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFP9188150.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6169 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaaa
//
LOCUS       DAZKCZ010000027        28108 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000027 DAZKCZ010000000
VERSION     DAZKCZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.027"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..763
                     /locus_tag="ACG8KN_001080"
     CDS             92..763
                     /locus_tag="ACG8KN_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188151.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            822..1262
                     /locus_tag="ACG8KN_001081"
     CDS             822..1262
                     /locus_tag="ACG8KN_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188152.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1274..1942
                     /locus_tag="ACG8KN_001082"
     CDS             1274..1942
                     /locus_tag="ACG8KN_001082"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9188153.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            2006..2629
                     /locus_tag="ACG8KN_001083"
     CDS             2006..2629
                     /locus_tag="ACG8KN_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFP9188154.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2685..3071
                     /locus_tag="ACG8KN_001084"
     CDS             2685..3071
                     /locus_tag="ACG8KN_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFP9188155.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3284..4675
                     /locus_tag="ACG8KN_001085"
     CDS             3284..4675
                     /locus_tag="ACG8KN_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFP9188156.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5286..5654
                     /locus_tag="ACG8KN_001086"
     CDS             5286..5654
                     /locus_tag="ACG8KN_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9188157.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5834..6775
                     /locus_tag="ACG8KN_001087"
     CDS             5834..6775
                     /locus_tag="ACG8KN_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9188158.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6844..7575
                     /locus_tag="ACG8KN_001088"
     CDS             6844..7575
                     /locus_tag="ACG8KN_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9188159.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7719..9899
                     /locus_tag="ACG8KN_001089"
     CDS             7719..9899
                     /locus_tag="ACG8KN_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9188160.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9919..10401
                     /locus_tag="ACG8KN_001090"
     CDS             9919..10401
                     /locus_tag="ACG8KN_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFP9188161.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10417..11178
                     /locus_tag="ACG8KN_001091"
     CDS             10417..11178
                     /locus_tag="ACG8KN_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9188162.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKVFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11309..12643
                     /gene="murC"
                     /locus_tag="ACG8KN_001092"
     CDS             11309..12643
                     /gene="murC"
                     /locus_tag="ACG8KN_001092"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9188163.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12950..13435
                     /locus_tag="ACG8KN_001093"
     CDS             12950..13435
                     /locus_tag="ACG8KN_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFP9188164.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13428..14111
                     /locus_tag="ACG8KN_001094"
     CDS             13428..14111
                     /locus_tag="ACG8KN_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9188165.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14237..16882
                     /gene="polA"
                     /locus_tag="ACG8KN_001095"
     CDS             14237..16882
                     /gene="polA"
                     /locus_tag="ACG8KN_001095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9188166.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16927..17763
                     /gene="mutM"
                     /locus_tag="ACG8KN_001096"
     CDS             16927..17763
                     /gene="mutM"
                     /locus_tag="ACG8KN_001096"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFP9188167.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17727..18356
                     /gene="coaE"
                     /locus_tag="ACG8KN_001097"
     CDS             17727..18356
                     /gene="coaE"
                     /locus_tag="ACG8KN_001097"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9188168.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18408..18740
                     /locus_tag="ACG8KN_001098"
     CDS             18408..18740
                     /locus_tag="ACG8KN_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9188169.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            19052..19543
                     /gene="nrdR"
                     /locus_tag="ACG8KN_001099"
     CDS             19052..19543
                     /gene="nrdR"
                     /locus_tag="ACG8KN_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9188170.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19566..20939
                     /locus_tag="ACG8KN_001100"
     CDS             19566..20939
                     /locus_tag="ACG8KN_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9188171.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20939..21865
                     /gene="dnaI"
                     /locus_tag="ACG8KN_001101"
     CDS             20939..21865
                     /gene="dnaI"
                     /locus_tag="ACG8KN_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFP9188172.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21994..22434
                     /locus_tag="ACG8KN_001102"
     CDS             21994..22434
                     /locus_tag="ACG8KN_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9188173.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22578..23279
                     /locus_tag="ACG8KN_001103"
     CDS             22578..23279
                     /locus_tag="ACG8KN_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFP9188174.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23276..24397
                     /locus_tag="ACG8KN_001104"
     CDS             23276..24397
                     /locus_tag="ACG8KN_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFP9188175.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24412..25644
                     /gene="pepT"
                     /locus_tag="ACG8KN_001105"
     CDS             24412..25644
                     /gene="pepT"
                     /locus_tag="ACG8KN_001105"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9188176.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25776..26684
                     /locus_tag="ACG8KN_001106"
     CDS             25776..26684
                     /locus_tag="ACG8KN_001106"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9188177.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26719..26991
                     /locus_tag="ACG8KN_001107"
     CDS             26719..26991
                     /locus_tag="ACG8KN_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFP9188178.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            27002..28048
                     /locus_tag="ACG8KN_001108"
     CDS             27002..28048
                     /locus_tag="ACG8KN_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFP9188179.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9456 a         4851 c         6116 g         7685 t
ORIGIN      
        1 aatagtaaat ttttatgaaa ttctactcta agcatcagac tttcttttca aaatatgcta
       61 ttttaaaaat gatgaaaaaa gggggagaaa aatgaatttt ttagttgttt taaaagatac
      121 tctgattgag cgatttacat ttctatatgt tcaattgcag gaaaataaaa cattgctgca
      181 tttttcacca gagttccatc tagaggggct aacattagga gaaatctatc aagcagaagg
      241 gctgtcggct gttttgcact tttttaaggt agaattggaa ttaccagtaa aagattatat
      301 cgagctttct cttgaatctt gggatcgagc agtggttgaa ctgtttccag aaggtttaat
      361 gatcgataca aacgatggga agatccatct gactgcagca gaactgcaaa aacaaatgcg
      421 ttatcaaata gatgacgaaa attctttttc tatttttaga cgtcagcaaa agattctaaa
      481 aagcttttta aaagtgatca gtaaaaaacg gaatctgatc aaaacgacac agctgttgaa
      541 aaaatatcca ggtctgcttc acacgtctat tcaatttcct caattgatta cgatgatcca
      601 tcgttttata cgtatgcaac agctaccatc taagaagctg tttcttccat tgacggacac
      661 atttcgagta gtcaacagag aagataaaaa gaaagtgatt gtgattgact ttaccagaaa
      721 tcggaatgtc cttcaccaag ttgcaatgga aaaggccaat taaggctttt tctttttgga
      781 tttttttgtc ataataaata ttagatgatg gaggagataa tatgatgcaa acatggttag
      841 aaaggctaat acgatttcag cagaactatc acaaacttat gcagggaaga tatgcgaaat
      901 ttgatcagtt gaatcgcggt ctattgatcg ctgcgcttgt ctttagtttg ctaagtcgtt
      961 ggttgcccta tagaatcgga caattgttgt tcatcgtctt tttcggatgg atgatatttc
     1021 gtttcctatc aaaaaaaatc taccccagac taaatgagaa tcaacgttat ttgaaatatc
     1081 ttgatagagt caaagaaaaa tatcatcaga taaaaaacaa aaagcaagtc aaaatggatc
     1141 gaaaaagtta tacttttttt gagtgcccga attgtcatca aaagcagcgt gcacctaaag
     1201 gaaaaggacg aatacgtgtg acatgcaaaa catgtggtat aaaattcgaa acgaatgttt
     1261 aagggagaaa agaatgaaat tagtattatt acgtcatggc gaaagcgaag caaattttga
     1321 aaattactgg acaggatggc tagatgtcgc cttaacagaa aaaggaaaag aacaagcagc
     1381 agttgccggt caaaaaatgc ttgaagccgg acttcaattc gatgccgttt atacctctgt
     1441 tttgaaacgt gcgattctta ccggacaagt cgcactagaa gaaatgggtc agttatggct
     1501 tcctataata aaaacatggc gtctaaatga acggcattat ggggatcttg tgggtaaaaa
     1561 caaagataag atgaaagaaa tatacggaaa agatcaagta aaaacatggc gcagaggata
     1621 tacggaagtt ccgccgttaa ccgcagaaaa tagatttgac cgcagatatg atcacttgga
     1681 tccgcatttg atcccatacg gagaaagtct ggaaatgaca gtgaatcgga tcattccctt
     1741 atggcaagat catctagcac caaaactaaa agatcggcaa gatttactag ttgtgggtca
     1801 tggtaatagc atccgagcac tgacaaaata tttagaagat gttcctgaag atcaaatgga
     1861 tacgatcgat atcccaaatg cacaaccaat ccagtataca ttagcagatg atttgacaat
     1921 tctggacaaa aaaatacttt aaagaacacc tttatctaaa agtgagagat agaaaaaaga
     1981 agcaaaggaa tggaacaaaa ggaacatgaa aaattatata tgggactttg atggtacatt
     2041 gtttgatacg tatcccgcaa tggtagatgg tgcatggcaa gcactaaaag attttggtat
     2101 cagtatggac aaaaaagaaa tctacttcaa aatgaaaaag tattctacta gttatttgat
     2161 caatgaatca aacttgaatg ccagagagtt taatgaatta tttcaccgat atgaaaaaga
     2221 aagtacggaa gtttcccgtc catttccaga aacgaaacaa gtactggaaa tgctaaaaga
     2281 caacggcggt cgtcatttca tcctgaccca ccgtttgaca gaatcaacat ggggcctgtt
     2341 aaaagaacat cgattggcac atctgattga ggaagtagta gggatcgatc aggactttcc
     2401 tcgtaaacct gatccagcat ctttaaatta tttgatcgat acttttcatt tagaaagaac
     2461 agacacgatg atgatcggcg atcgtcgttt agatatcgaa gcagggaaaa atgcaggagt
     2521 cgctacttgt ctttacgaca tcgatcattt tctgggtgag atcccggcag attatgtggt
     2581 aggaaatctg aatgaaatcc ttaccttgca gagcatcatt aaaaagtagt atcccctttc
     2641 aaaggagata tgttataatt ttcataaagg agtgataaaa ttatatggaa atcagaagac
     2701 aaaaagaagt tgtagaagca tttcattatg atatgagatt gccgaaccaa gaagtagaaa
     2761 cagatttgaa agtaggcttc tcgcctttaa aagaaacgat cgaaaactat ccgaaagaac
     2821 atagcatcgt aggagcacgc ttagagttcc gtttagtgtt tgatacatat gttttatctg
     2881 gaagtgtgag tcagatcaat catatcctca atcggaaaat tgaaggtcaa aatgatgtca
     2941 cacaagaaga agtggacgag ctagtagcac cgttattcga tatcgtacgc cgaatgtctt
     3001 atgaggtgac agagatcgcc ttagatcagc ctggggtaaa attgaatttt caatcagaaa
     3061 aacaagaata gacagtaaaa acatacctgc gatagattaa agctatcaca ggtatgtttt
     3121 tttattacta attgtaagca aaaagaatgt catttcgata aaaatcgtct aaaatataaa
     3181 agtcactgaa aaaaattttt tcaggaggat ggttgaagaa tcattctcgg gaacctttac
     3241 ttccttcgat ggacttgtca ttaataatat gaggaggaga aaaatgacaa cactcatcac
     3301 agttttagtg gttctagttt ttgcggcatt attatatgtg ttctatcgta tgcaaaagcg
     3361 ccatacaaaa ttttcaacac gcgtctttac agcattagga gttggtatcg tctttggcgg
     3421 tctgcttcaa ttgatctttg gtgtagacga caaagtcgtt actcaatcaa tggaatggat
     3481 cgggattgta ggtcaaggat atgtatcatt cttgcaaatg ctagttatgc ctttagtgtt
     3541 tatttcgatc attggtgcat tcacaaaaat gaaagtttct gaaaagctag ggaaaattag
     3601 tgcgactgta ttgacttctt tacttggaac aacagctatt gcagcattga tcggaattgg
     3661 ttcaacaatg ctgttcggac tccaaggagc taaattcaca caaggagctg cagaaacagc
     3721 acgaatctct gaactggcaa cacgtcagga aacagttgaa aacttatcga ttccacagca
     3781 gattttatcc tttatcccta aaaacgtttt tgctgattta gcaggtagtc gtgcaacaag
     3841 tacgattgga gtcgttattt tcgcggcatt tgtcggaatc gcttatctag gcgtgaaaag
     3901 aaaagcgcca aaagaagcag cattcttttc agatttagtc gacagtctgt ataaaatcat
     3961 tatgcggatc gtgacgcttg ttttgcgttt gacaccttat ggagtatttg ctttgatgac
     4021 aaacgttttg gcaacaagtg attttgcagc tatcgtcaat ttaggtaaat ttgtcgttgc
     4081 ttcttacgta gcattgcttg ctgttttgat tgtccactca ctgattttga ttggtgcaaa
     4141 agtcaatcca atcaactatt tcaaaaaagc ctttccggta ttgagctttg cgtttacttc
     4201 acgttcaagt gctggagctt tgccattgaa tatcgagact cagacaaaag ctttaggagt
     4261 agatgatgca acagctaatt tctcagcaag ttttggtttg tctattggac aaaacggctg
     4321 tgctggcgtt tatccagcta tgttggcaac gatcgtcgca ccagcagctg gagtgaatgt
     4381 ctttagtttg gaatttattt tgatgcttgt ggcaattgta acaatcagtt cttttggtgt
     4441 agctggagtc ggtgggggcg caacatttgc ttcactgatc gttttagggg caatgaactt
     4501 gccagtagca atcgttgggt tagttatctc tgttgaacca ttaatcgata tggcacgtac
     4561 agcagtaaac gtcagcggta gcatggtagc tggtatcgtg acaagtgcac gcatcaaaga
     4621 tttagatcgc gatgtcatca atgatgattc taaagtgatc gatgtgaatg catagtcaaa
     4681 aagaggttgt gatagaagtg gtcagcttta agaaataaga agaagtttcc taaaaatagt
     4741 ttctcaaatt tttgtgaaat ttcggcttat ttccgagaag ctacttctga aacaccgtga
     4801 ataagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
     4861 ttcctcatat tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt
     4921 ttaagaaaga ggtcgtgagc ctgccgtttc actctgagtc acaaagagca atttcaaaaa
     4981 atagcttctc atgtttgttg aatattgtgt cttgtgaccg aggagttgtc aggagaacac
     5041 cgtttaccag aggttgtgac ttgtttttag tcacgacctc tttttttatt tattccatat
     5101 aaatacacat tatttattcc gaaaagtgaa agcgcaaata acaaaaacaa ccaacttatt
     5161 tattattaaa gaaaagcgta tttaagtagg acgattctgt tgagagcttt cgtagatttt
     5221 cccattttcc tatggtaaac tgacaaagtt gagaatgtta gaacagatcg tttaggagga
     5281 aacagatgtt tacgaatgaa ataaaaatca tgctttatgt ggacgatgta gaaaaaaatg
     5341 caacgttttg gagagcagtt ggctttgcgg aaaaagatcg tcaagaaatg gatgggacgc
     5401 ttataattga agtaggagtt gaggacagtc aaacctcatt tctgttatat gatcgagcat
     5461 ttgttgagtc ccattcacct ggcacagcta ctagtgcccc atctttgatg ttttacagta
     5521 aagatatctt tggactatat aaaaaaatgc agacgcaatc agtcacatta ggtgatatgg
     5581 tacagcttgg agaagaatat gtctttaact ttgctgatcc cgatggcaac tactttgcgg
     5641 ttacaggtaa ataatagaaa taaagacttt gtttttctgt gcgaaacagg aaagcgaagt
     5701 ctttttaatt caaataataa tattatgtaa acaaaaaact ttccatgcgt aattatacac
     5761 aagcaaataa atcgtcaaat gcgaacgcct tctttatact gtttaggaac gaaaagaagg
     5821 aaggaagaaa tacatggatt atcaagtact gctttattac aattatacaa aaatcgaaga
     5881 accagaggaa tttgccaaaa atcatttagc cttttgcaaa tctttggatc ttaaaggtcg
     5941 tattctagta gctgaagaag gaataaatgg tactgtgtct ggaaccgttg aaaacacgac
     6001 tgcctatatg aaagcaatga aagctgatcc tcgatttgct gatacgatgt tcaagatcga
     6061 tccaagcgaa ggacatgcct ttaagaagat gtttgtaaga taccgtccag agcttgtaag
     6121 tttaaagcta gaagaggaca ttgatccaaa ccaagtgaca ggtgcctatc tttcaccgca
     6181 agaatttaaa gaagctattt tagatgaaca gacagtggtg attgatgcac ggaatgatta
     6241 tgaatatgac ttgggacatt ttcgaggggc tgtacgccca gatatacgca gttttcgaga
     6301 gcttccagac tggatccgcg agcacaaaga agaatttatg gaaaaacggg tagtgaccta
     6361 ctgtactggt ggcattcgtt gcgagaagtt ttctggttgg cttgtccgtg aaggattcaa
     6421 agatgtcgga cagctgcatg gcggtattgc tacttatggt aaggatcctg aagtccaagg
     6481 agaattatgg gacggcaaga tgtatgtgtt tgatgaacgg atcgctgtcg atatcaacca
     6541 agtagataaa caaatcgtcg gtcgcgactg gtttgacgga acaccatgtg aacgatatat
     6601 caattgtagc aatcctgagt gcaatcgtca gattcttgct tccattgaaa atgaagaaaa
     6661 acatttagga gcatgtagca tagagtgtgc gaaacatccg cgcaatcgct atgttcaaga
     6721 ccatggaatc tctccagaag aagcagaact gcggtttaca gctttaatta aatagaaaac
     6781 aatagttttg attgtcgacc atttccaata tgcgctatac tagcgttgag gatggtgatc
     6841 cacatgaaat cattgattta tgctattggg gatgtccatg gcaaacatga gctttttgaa
     6901 aaacttattc actattatga tccaaagatc catcagcttg ttttattagg ggatctcaat
     6961 gatcgaggtc ctcgtacaaa agaatgtttt ttattaggaa agcaattagt cgaagaaaca
     7021 ggagccattt acctccgggg aaaccatgaa gaatattttt tgcaatttct gcaatcaccg
     7081 gaggactggt atgcgcctta tatgagaaat ggtggaaaag aaacgtttga aagcctgatt
     7141 cacccagggg catcggagga atactcccca actgaaatct cgatgatgat ccaatcgaga
     7201 tattcagaac tgattaaatt tttagtccag cgccccttgt attatgaatg ggaaaattat
     7261 ctttttgtcc atgccggtgt ggatttgaca aagaaagact ggcgtaagac aagtctaaga
     7321 gactttattt ggataaggga accatttcat gaagggaaaa acaatacagg taagacaatt
     7381 gtatttggac atacgattac accaatgctc catggagata tgcagacaac agacctctgg
     7441 attcaggatc ataaaatcgg aatggacggt ggggctgtat tcggagggtc attgcatggg
     7501 gttgtttttg acaaggaagg tattcttcaa gatattgaat atcagaacaa cgaaggacct
     7561 tggcatggaa tctgactaat ggaaagaatg aaggagctgt gactttgttg cggctctttt
     7621 tatttttata gagaatattg tttctttcag tagtttaacc caggataaaa agtatgatag
     7681 aataaagaag caatttttag cagaaaagag gcattttaat gacggaaaat ttgaaccaga
     7741 cgatcattca gcttgtacaa aaagaacttt cagactatcg tcctaagcag ttgactactg
     7801 tacttaattt gttaaatgaa ggaaacactg ttccttttat cgcgcgttac cgaaaagaaa
     7861 tgactggaag tcttgatgaa gtccagattc gtgaaatcga agagcggtat gcctatcttg
     7921 aaaacttaga aaaacgtaaa actgaagtga ttcggctaat cgatgaacaa gggaaactga
     7981 ctcccgagtt agaagcagaa atcacccaag ccgtgaaaat gcaacaagtg gaagatttgt
     8041 atcgtccata caaacaaaaa cgacggacaa aagcaacgat tgctaaagaa aaaggacttg
     8101 aaccactcgc gaagtggctg atgcagctta cagatggaga ggtgcaatct gaagcagaaa
     8161 aatatattga caaagaaaaa gaagtctctt ctgccgaaga agcgttgcac ggtgctcatg
     8221 agattattgc tgaacaagtc agcgataatg caaaatttcg gacttggatt cggtcttata
     8281 cgtataacaa aggtatgtat gtcagcaatg tcaaggatga gcaagcagat gaaaaaggcg
     8341 tatatgaaat gtattatgac tttgcagaac ccgtccataa aatggtttct catcgaatat
     8401 tagctactaa tcgtggagaa aaagaagata ttctgaaagt attctttcaa gtagatgaag
     8461 cagcaatttt agcttacttg gatcgccaaa tagtcaaaaa tcctgcctct ccttcttctt
     8521 cattcgtcag agaagcctat caggatagtt ataaacgctt tattcagcca gcaatcgagc
     8581 gagaactgcg caatgaattg actgaaaagg cagatgagca agcaatcgct attttcggcg
     8641 aaaacctccg taatcttttg ttgcaaccgc cactaaaagg taaagttgtc ctgggatttg
     8701 atccggctta tcgtactgga tgtaaactag cagtcgtaga tgcgacaggg aaagttttag
     8761 caatcgaagt tatttatcct cataaacctg cagcgcaagc aaaaagagaa gctgctggac
     8821 cagcttttat ccaattaatt aataaatatc aagtagatat ggtagctatc ggaaatggta
     8881 cagccagtcg ggaatctgaa ctttttgtgg ctgagcagtt gaaatccgcc gaccataaaa
     8941 cctattacgc tatcgtcaac gaagcaggcg cttctgttta ctccgctagc gaaattgcac
     9001 gaaaagaatt tccgcatcta caagttgaag aaagaagtgc tgtttctatt gcccgtcgcc
     9061 tgcaagatcc tttagctgaa ttagtcaaaa tcgatcctaa agctgtcgga gtaggccagt
     9121 accaacatga cgtttctcaa aaacgtttgg ctgaacaatt ggattttgtt gtggaaacag
     9181 cggttaacca agtaggtgtg gatgtgaata cagcaagccc gcagctcttg cagcatatat
     9241 caggattgaa taaaacaact gcccaaaaca ttgtcattta tcgtgaagaa aacggagaat
     9301 tcaccgcacg aactcaattg aaaaaagtgc cacgcttagg accaaaagca tatgaacaag
     9361 caatcggatt cttgcgagtc cctggtggga aaaacatttt ggacaacaca ggtatccatc
     9421 ccgaaagtta ttctattgca aaagaaatat taacttcagt ccaactttca gaaaaagagc
     9481 ttggtacaga agaagcagta gaaaaattga cacgattatc cgtagagaaa ctggctgaaa
     9541 gcctatcagt cggagaagaa acgttagccg atattttagc tgggctgaca caacctggac
     9601 gagatatgcg tgatgagatg ccagcgccct tattacgtac agatgtattg agtatggaag
     9661 acttgaaacc agggatggaa ttgactggaa cggtccgtaa tgtcattgac ttcggcgcat
     9721 ttgttgatat cggtgtgaag caagacggac ttgtccatat atcgaaactc agcaaaaaat
     9781 tcgtcaagca tcctacagat gtcgtgtcag taggagatat cgtaacagtt tggatagaac
     9841 aagtcgatac gaaaaaaggc agaatcagtt tgacgatgct ttccccttat gaagagtaac
     9901 aatgaggagt aacaaaaatt gacacaagaa gaactgcagc agttagtaga agagatatca
     9961 ttaaaaagct tccagcttcc tttcaagcac caagcaatgt tcaatccacg cttacgtaca
    10021 actggcgggc gctatcattt aaacgatcat catttagatt ttaatgcgaa gctatttgaa
    10081 gagtcagacg aagagacgat tgcaggaatc atcaaacatg aactttgcca ttatcatctt
    10141 catctagctg gaagagggta tcagcataaa gatatggatt tcaagtatct tttgaaaaaa
    10201 acaggagggt tgcgctatgc accagccttg acgaagaaac agataaagat ggagtattat
    10261 cgatgccaag attgttctgc cacgatctgc cgcaagagaa gaatcgatgt acgcaaatat
    10321 cgttgtggaa gatgtcatgg agaaatcaaa tggattgaga caaaaagaga agactttaat
    10381 gaagcacaaa aaatcagatg aaaggataga aggcctttgg aaaaaagtga gcagaggaat
    10441 ctaaaagact ggaatcagct attaaatttt ttagccgcac ctgaagaggg aaattttgag
    10501 gaactgacgg cacctacact tattttagct ggaaactgtc ttcctatctt agctgataaa
    10561 gcagcacaaa tgtatttaaa tggccaagta gatcaattgt ttttggtcgg aggggtgggg
    10621 catgcgacta gaatccttta tgagaatttt gaaaagcaag gatttcattt tgaagatggc
    10681 atgagtgaaa gtgaaatctg tcgtcagtat ctaaaagaag tctatgattt gcccgataag
    10741 gtttttctga tagaatcgaa gtctacaaat tctggagaga acgccacttt ttcactggag
    10801 attcttcatt ccttaggagc tgtaccagaa aaaatcttat tgatgaatga tccgacactg
    10861 caacgcagga ctcgagcaac ttttgagaaa gtatggcaaa atgaacaaac tattttcgcg
    10921 aatgctgtgc catttgtgcc ggaaatcctt cacttttctg aagagatcat ctttacagca
    10981 aaagaactga atcatcaatg gcctaaggaa tatttccatg cgctagtatt aggagaaatg
    11041 gaacgtctcc atgacgatga gaacggctat ggtccgaaag gaaaagattt tattcctcac
    11101 attgatatat cagaggaagt ttggtcttct tacaccagaa ttaaagagtc aataaaaacg
    11161 gatttttctc gtacttgacc attttagcaa gcgaatcctc ctttccaaat aaatttggtt
    11221 gaaattttca atcgtttata agataatgag gagcgtgttg aatgtaatag ctaaaagaat
    11281 ggacgctaga taagagaaga ggattcaaat ggaaaatcaa aataaattgt atcattttgt
    11341 tggtatcaaa ggttcaggaa tgagctcctt ggctcttgtt ttacatgaac aaggtctaaa
    11401 tgtccaagga tcagacattg aaaaatattt ctttacacaa agagatttag aaaaagcaaa
    11461 tatcacgatt ctgccattta acgcagataa tgtaaaacca gggatgacga tcattgcagg
    11521 aaatgcattt cctgattcac atgaagaaat ccagcgagca aaagaattag gtttggaagt
    11581 gattcgctat cacgacttta ttggtcattt tatccaaaat tatacgagta tcgctgtaac
    11641 aggctctcac ggaaaaacaa gtacgactgg actgctctct catgtattga gtggtgtccg
    11701 tccaacaagt tatctaattg gagatggaac aggacatggt gatccgcaag cggaattctt
    11761 ttcatttgag gcctgtgaat atcgccgtca tttccttgcc tattcaccag actatgcaat
    11821 catgaccaat atcgattttg atcatccgga ttattataca agtatcgacg atgtatttac
    11881 agctttccaa acaatggctg gacaagtgaa aaaagctatt tttgcgtatg gtgacgatgc
    11941 ttatttacgc aaattaaaag caaatgtgcc gatttattat tatggtgtca cagaaaacga
    12001 tgatatccaa gcacgaaaca tcgaacgcac aacttctggt tctgcttttg atgtctatca
    12061 tggggatgaa tttgttgggc acttcactgt accagcattc ggtaaacaca atatcttaaa
    12121 tgctttaggt gttattgcag tagcttattt cgaaaaactg gatctgaaag aagttgcaga
    12181 agaaatgctg actttcccag gagtaaaacg tcgattcagt gaaaagatcg tagcagacat
    12241 gaccgtagtc gatgattatg cgcatcaccc tgcagagatc aaagcaacga ttgatggcgc
    12301 acgccaaaaa tatccagata aagaaataat tgcagttttc caaccccata cgtttacgcg
    12361 tacgattgca ttgatggatg aatttgcaga agcacttgat ttagctgata aagtttactt
    12421 atgcgatatt tttggttcgg ctcgggaaga acaaggaaac gttaagattg aagaccttgg
    12481 agcgaaaatc aaaaaaggtg gagaagtcat caaagaaaat aatgtatctc cattgttaga
    12541 ttatcatgat gcagtagtaa tcttcatggg tgctggagat gttcaaaaat ttgaacaagc
    12601 gtatgaaaaa cttttaagca gcacgacgaa aaatgtactg taatacaatt actgcagtgt
    12661 ccttataaaa atagaaagag ggggagtggc aaaaatcttg ccaagccccc ttttttgcaa
    12721 cttatgataa acggtgttca aaagcttgca tctgcggtaa taagcccaat caaatcaaaa
    12781 acatgagaag ctgttttttc ttggctgttc ttattatttg atgcaggaca gctttttcac
    12841 gacctctgta aactatagga atctgttaaa tcaagggagc agaacttgcc ttttacgcta
    12901 cttttggtac aataatgtat gattaaaaat aaactggagg gtttctatct tgaacatagg
    12961 taagccgaga aaactaggaa aaacaataaa tgaaatcgag gaaggcgact cgttatcctt
    13021 aacagaatct attgaagata accagctttt gctgtatttg ggattgacga atgatgcaaa
    13081 tcctttgtat atccaacatg actttgcaca aaaaacagaa tatcaacagc caatcgttcc
    13141 atcagtgatg ctgatgggga tcatcacaag tgcaatttca aagcatcttc ctggaccagg
    13201 atcacatgtt gtcaattttt ctgtcaattt tattgaaccg gttttccatt atgaaacact
    13261 gacttttcag tttgaggtca taaaagtaga taagatgaag gatgtggtga cgatttctgt
    13321 agaagcggag aatacgaacg aagatcgtgt gttggatgct gtcgttatgg tccaaccgcc
    13381 acaagttcct gaattggaaa tagcaatcga aagtgaaggt gaattgaatg aataatccaa
    13441 gtacatcaat gtcaggtatc aatcgttttt atgcaaaagt ttatggattt ttaggtatgg
    13501 gacttgccct tagtgcagtc acttcttact tggctctttg ggtatttcct ttgcaattag
    13561 caacttttat caataatttt ccactaggct ttgtgggtct gtggatcgtt gaactgattt
    13621 tagtaatggt tctaggcgta aaagcacaaa agaatccgag cttagcaatc ggtggattta
    13681 ttgcctactc tcttttgaat ggcataacat tagcggttac tcttgcatac tacgatattg
    13741 gaacagtaac gcaagctttc ttgacagcta ctttgatgtt catggggatg tctgttgtcg
    13801 gagtaacaac aaaaagagat cttagcgggg ttggaagagc aggacaggca gctttaatcg
    13861 gagtgatcat tgcactagtc ttgaacatct tcattttgag aagtagcgta gttgagctgt
    13921 tcatctccct actgttagtc gtcatctttg cagggattac agcttatgat aaccaaagca
    13981 tccgtaatat ctatgtccag acaaacggaa cagcacatag tggaattgct gtattcatgg
    14041 cattgaagct gtatttggac tttatcaact tgttcttggc gtttctgaga atctttggac
    14101 ggaacaacta aaaaagaaaa aaattcatca gttggggcta taccgaatta tttggtatgg
    14161 tcccgctttt tcttttcgca ttccgcagga ttctttgtta gaatgaataa gaactttaca
    14221 gtaaggagtt aagaagatga caaaaaataa attattactc gttgatggga acagtgtggc
    14281 ttttcgcgca ttctttgcgc ttcacaattc tcttgaacgt ttcaaaaata gaaacggtct
    14341 tcatacaaat gcaatctatg cattcaatac gatgtttgaa aatgttatgc aaaaagagca
    14401 accgactcat gttctggttg cgttcgatgc aggaaaaacg acttttcgga cagaaatgta
    14461 tgcggagtac aaaggtggaa gatccaaaac acctggcgaa ttcaaagaac aaatgccata
    14521 tatacgtgat ttgttgacag gactaggggt acagtattac gaattgccca attatgaagc
    14581 agatgatatt atcggtactt tggcagaaaa agtagacaaa gatcaatttg atgtcgtcgt
    14641 gctttcaggt gatcgggatt tgactcaatt agctagtaaa gaagtaaaag tggatatcac
    14701 tgtaaaaggt gtcagcgata tcgaaagcta tacaccagaa catgtagcgg aaaaatatga
    14761 tgggttgacg ccaaaacaga ttattgacat gaagggacta gccggagatg cctctgacaa
    14821 tatccccggt gtgactaaaa tcggtgaaaa aacagctatc aagctattga aacaatatgg
    14881 ctcagtcgaa ggaatttatg aacatatcga tgaaatgaag caaagcaaaa tgaaagaaaa
    14941 tctgataaat gacaaagagc aggctttctt atcgaaaaaa ttggcgacca tcaatacttc
    15001 agcgcctgtg gatgtctcta tcgactcatt gaagtatgaa gggaaaaatc tcgataaact
    15061 cgttccgttt tataaagaaa tggatttcaa tcagttcctt tctaaattga atatcgtaga
    15121 agaagaagta aaaatggatg atattttgtt tgaagtcgtt catgaattca aagaggaaat
    15181 gtttactacg gatatggcgt tgtacgtgga aatgatgggc gacaattatc atacagagga
    15241 aatcgtcgga gtagcgtggg ggacagaaaa gaaaatttat gtcacgaacg atctatctgt
    15301 attcgaaaac cgagcgttcc attcttggat cactgatccg actcggctga aaaaagtata
    15361 tgatgcgaaa cgaacttatg ttgctttaaa ccgctatact ggaaaaacga aaggaatcga
    15421 ttttgatgtc ttgctaggtg cataccttct cgatacgaat gaaaaaagca cagatatcga
    15481 aggaatagct gctcattacg gatacaacga tatccaatca gatgaagctg tatacggcaa
    15541 aggggcgaaa aaaggtttac cagaagaaga ggaactcttt tttgctcatt tagcgcgaaa
    15601 agtagctgca atcaatgcgt tgacaccaaa attgagccaa gaactggcag aaaaaaacca
    15661 agaagatctt ttccggaaaa tggaactgcc attgtctatc attttggcag agatggaaat
    15721 aagcgggatc aaagtagatg cgactcgctt acaagaaatg aagggcgagt tctctgctcg
    15781 cttaagagag atcgaacaaa aaatctacga agaagccggc gaagaattca atttgaattc
    15841 accaaagcaa ttaggagtga tcttatttga aaaaatggga ttacccgtta tcaaaaaaac
    15901 aaaaactgga tattccacag ccgtagatgt actggaacag cttcgtgaac aagcaccaat
    15961 cgtagaggat atcctcacct atcgtcaaat tgcaaaaatt caatctacct atgtcgaggg
    16021 actgctgaaa gtgatccaat cagatggaaa agtgcatacc cgttatgtcc agacattgac
    16081 ccaaacagga cgattaagct ctgttgatcc taatttgcag aacatcccga ttcgattaga
    16141 tgaagggaga aagatacgtc aggcatttgt tcctagagaa aaagactggc tgatttattc
    16201 ttctgactat tcacaaatcg agttacgcgt acttgcgcat atctcaaatg atgaacattt
    16261 gaaagctgcc ttcctagaag gtcaagatat ccatgccagc acagcgatgc gtgtatttgg
    16321 aatcgaaaag gcagaggatg tcacaccaaa tatgcgtagg caggcaaaag cagtgaattt
    16381 tggtatcgtg tatggcatct ctgattacgg actttcacaa aatctaggaa tcagccgaaa
    16441 agcagcacag caatacatcg atacgtactt tgaaaaatac ccaggtgtca aagaatatat
    16501 ggaaagaatc gtacgcgaag caaaagacca aggctacgtc gaaacgctgt atcaccgccg
    16561 tcgatatctg ccagacatca attcaagaaa ttacaatatc cgttcttttg cggaacgaac
    16621 agctatcaat acaccaatcc aaggaagtgc tgctgatatt ttgaaaatcg cgatgatcga
    16681 attggataaa cgattaaaag aaacaggtct tcaagctacg atgctgttac aagttcatga
    16741 tgaacttgtc ttcgaggtac cggaaaaaga acttgaatca ttagataaat tggtgaagga
    16801 agttatggaa caagctgttt ctcttcatgt accactgatc actgacagca gttggggaaa
    16861 aacgtggtac gaagcaaaat aaacattgtt taagacagag caatataatg aaggtgaaaa
    16921 gaaaaaatgc cagaattacc agaagtagaa accgttcgta aaggattgga acggttagta
    16981 gtaggaaaaa cgattcaaaa agttcaggtc ttgtggccca agatcatcga gcaaccagag
    17041 acacccatct ttgaggcttc tcttgttggc gaaaccatcc aatccattgg acgtagaggg
    17101 aagtttttaa tttttcatct cgatcattgt gaactgatct cacacttgcg aatggaagga
    17161 aaatatcaat ttacgaaaga aaatacacca atcgataaac atacgcatgt acttttcttt
    17221 tttgaggatg gaagccaact tcgctacaac gatgtacgaa aatttggtcg tatgacaatc
    17281 gtcgaaaaag gagcaagtgc aacttaccga ggaatcatga aattgggacc tgagcctttg
    17341 ccagatagct tcttattagc agatttcgca aatggactga aaaagtccca caaagcaatc
    17401 aagccattgc tgctagacca acgtctagtg actggtctag gtaatattta tgtagatgaa
    17461 gcattatggg aagcgaagat ccatccagaa caaccggcga acacactgcg ccccaaagag
    17521 gtagaacaat tacgtctatc gatcattgat gttttggatc gtgcaataga agccggaggt
    17581 accacgattc gcagttattt gaatgcatta ggagaatcag gcggatttca agtagcactg
    17641 catgtctatc agcaaactgg caaaccatgt atacgttgtg gaacgccgat cgtgaaaaca
    17701 aaagtggcac aaagggggac gcactattgt ccgaattgtc aaaggctaaa aatcgcaaag
    17761 taggatttgt tttaggtttg acgggtggaa tagctaccgg taaaagtacg gcagccaaag
    17821 tctttcagtc tcatggattt cctttgatcg atggcgatgt gattgccaga gaaactgttg
    17881 agcctggaac gcttgctctg aggaaaattg tatcagtgtt tggccaagag gtattgcagc
    17941 ctgatgggca actagatcga gggaaacttg ggatgattgt ttttccatca aaggaaaaac
    18001 ggcaaaaact tgatcagcta ttagatccgt ttatccgaaa agcgataaaa gatcaaatcg
    18061 cctctctaag ttccaatcat cctttagtaa ttgtagatat cccattgctc tacgaaggtc
    18121 attatgacca ctatatggat gcagtagctg tcgtttatac tacgccagaa actcagcttc
    18181 agcgtttgat gaaacgtaat caactgacgc tagaacaagc gcagcagaga attagtagtc
    18241 aacttccaat cgaagaaaaa aagcagcgtg cggatatttt gttcgacaac aatggaacaa
    18301 aagaaatact tgtgggacaa atcgaaaatt ggctttttgc taatcgcttt ctttgagtat
    18361 ctgtgtcaat tttgctatcc tgagtatata gaaagtgagg gatagcgatg ggatcattaa
    18421 aaaatcaaaa ggataaagtc gtaggtaacg caaaagaaaa aatcggcgag atgctcaata
    18481 aagaaaatct aatcgatgaa ggtcgtacgc aatcagaaac agcagaagaa aaagaaaagc
    18541 agtaccaagc aagaatcgat gaaatagaag accagcaggc agaagaagaa gcgaagcggc
    18601 aagatttgac tcaagaacga aaaaatccga cacctgatcg tagtaaaaca ccgctcccag
    18661 aaagtgacca cattcccgat gagcaaaaaa tcgaggaaca gcgaatgaaa cactatacag
    18721 gaatcgaaga gaagttataa aatagtaaaa aatcgagatg tgacttgaag cagatgagaa
    18781 ggtgttcatc tgcttcaagt tttttgtgct agatgactaa atcagctttt gtgtaagtta
    18841 tttctcagta atcaaggtga tcagtattgg aaaattgaaa tttagtaagg acaggggaca
    18901 caattacgca tgaaagtaga aaaaattgtt cttatatccg tgttttttgg gtaggacagt
    18961 cgcgtttgtg atttgtttcg tgttataata caactagttg taaagaaaga cacatccttt
    19021 atataaaatg cgatgcaaga ggtgagacaa aatgagatgt cccagatgtc aacataataa
    19081 ttcacgagta atcgacagcc gtcaagcaga tgatggacgt gcgatccgtc gccgtcgcga
    19141 atgtgagaat tgttcgttcc gattcactac atttgaacga attgaagctg ctccgttatt
    19201 agtaatcaag aaaaacgggg aacgcgaaga gttcaatcgc gataaaattt taagaggact
    19261 catccgttca gcagaaaaaa gacctgtagc tatggaacaa atggaacaaa tcgtcaacca
    19321 tgtcgaaaac cgcgtacgca gtctcggaga aaacgaagta tcgacgaatc taattggtga
    19381 atatgtgatg gaagatttag ttgatttaga cgaaatcgct tatattcgtt ttgcaagtgt
    19441 ttatcgccag ttcaaagata tgtctgtttt cttaaaagag ttgcaagata tcgtggataa
    19501 agcaaaagaa aataataaag agaataataa agaaaacaat taagaaacaa aaaggaggca
    19561 tttccttgga aaatgcttgg aaagagcttc aaccaaaaaa tatttaccaa ggaagaaaag
    19621 cggctccgct gagagaagac agcaagaacg ccttgctgta tctttatcag ccactaatcg
    19681 gcggagaagc cttgagtatc tatttcaccc tgttatcaga aatctctcaa gaatctggca
    19741 aaggtcctga agggatgcat gcagatctgc ttagtagtct aggctgcggt atcccgcaat
    19801 tttatgatgc aagaaaaaag ctagaaggaa tcggcttact ggatgtttat tttaaagaag
    19861 atagtgatct gggacctcgt tttatttatg agctgttaga accactcatt cctaaagcat
    19921 ttttcaaaga ctctgttctg gcttttcttc ttctggaaaa agttggtgaa cgacgattcc
    19981 agaagcttac ccaattcttt gaacctaaaa ggcatacagc agagggatat caaaaaatca
    20041 caaaaaaatt tttagatgtt tatcgcttca atgaagcagt gtacgctgct aatcaagaaa
    20101 acatcgaatc gctgaataaa tcttttgaag aatctgaatc agtagcatta cttgaagcag
    20161 attcgacatt cgattggcag tttttgaatg attggctggc aaaaaaacat attgcaagtc
    20221 ttgatgatcc aatcatcaaa caagtcgaca tgtatcatca gctttacggt ttcgatgaat
    20281 tgacacttgg cgaactgatc agtcaatctt atgactttag tgaacaaaaa gtatctgaaa
    20341 aagaactgca aaaagccatt ttggcaatgc agagacgtcc ggtgaagcaa acaaaagaaa
    20401 cagttgcggt ttctgaaaac agtaatgaac cctcgtcaaa cgaacatttg acaaacgcag
    20461 cgcttcagct gataaaagaa gcacagagca ttccaccgat gaaatatctg gaagcaatca
    20521 agtcagaaaa aggcggatat gtttctaaac aagaaaactg gttactgcaa gatcttgttt
    20581 ctcaatcagg tctttccagt agcgtgatca atgttttgct gaattatgta ctagtgatca
    20641 agaataatgc tagcttaaat gccagttatg taaatacagt ggcaaatgaa tgggcacaaa
    20701 agaaaatcgc cacagcagaa gaagcgatcc agcacattcg gcaaataagt aatcaagcaa
    20761 aacaatcaaa acaaaagaag caaacaaatt atcaaactgg caagcgaaat gttcgacggg
    20821 aaaagcttcc tgattgggtc aaccagccaa aagatgaaaa acagatcagc agcgagaaaa
    20881 aagcagagat tgaccgccgt tttaaagaat acctggcaaa aaaggagggt gatatctagt
    20941 ggaagacgta ggcaaagaat tgaagaggat catcagcaaa cgcaattatc aagaaaagta
    21001 tgctgaaatg atcgctgaag tcttaaatga tgcggatgtt caagcttttc taagcgcaca
    21061 ccaagaagaa ttgacacaag cggatattga aaaaagttac gctaaattgt atgaattcgt
    21121 gcaagaaaag cgtaaatatc aaaaaaacga tccaacgatg atcgctcccg gctatgagcc
    21181 gaagctgacg ttgaattttc attatgtgga tgtcacttat gtaccaacgg aagcattgtt
    21241 agcacggcaa agacaagaag aaatcagaag ccgagtaaaa gcaatggata tgccaaaaga
    21301 tattcaatcg gcttcttttg aaaactttga acgtacagat ggacgaggat atgcgagctt
    21361 agaagcccta gattttgtag agaaatatac tgctgatcca aaagggtatc ataaaggact
    21421 gtatctagta ggaagttttg gtattggaaa aacatattta ttaggagcaa cagcgagaga
    21481 attagcgatt gctggattca ccacgacgct agtccatttt ccgacatttg ccgttgagat
    21541 gaaacaggca atcggaaaag atcaagtggc agaaaagtta gatgcagtga aacgttcacc
    21601 tattttgatg atcgatgaca ttggtgcaga tgcgatgagt agttggatac gagacgaagt
    21661 attcggtgtt atccttcaat atcggatgca ggaacagtta ccaaccttct tctcatcgaa
    21721 ttttacaatg gatgaattgg aacagcattt gtccgtcact caacgtggag atgaagagcc
    21781 gctaaaagcg aaacggataa tggaacgcat acgttatttg acaaaagaaa tcgaaatgac
    21841 tggtcgcaac cgacgcaatg gttgaacagt tgacaagtcc aactgacatt tgacctagta
    21901 gtaaaagcag gtcagcgaat cattatatgg acagaaatga ccggagacaa tcaccgcaac
    21961 aagtaatgca gaaacttaaa cggaggattt tttatgaggg ctattttttc tagtatatct
    22021 gtagttggtc tattttttct tttagcaggc tgcggtagcg cagatagcaa cacaggttct
    22081 gttgattcga ttgatgcggg tcaaacagta aaaacaagca gtacgaaaac ggagagacaa
    22141 agcatatttg tcggaacgat tgaaagtatc actcaagtag aagcagaaga agaaacgctt
    22201 cagttgaaac tcgtcgatgt catggccaaa gaagatccag cgaatatggg aacgtcattc
    22261 agcaatgacg gagtgatctt aaatgctact gctgaaaaaa taagcgtgga cataaatgaa
    22321 ttgaaaatcg gtgacaaagt ccaatttaca ttagttgaga tgccgatcat gacgatgtct
    22381 atcccaccgc aagttcctgg agattcgatt attgatgttt cattgaacaa ataaccaata
    22441 acattttttc aaaaataaga aaatgttgtt ttatagaacg aaaaggtata ttttttaagg
    22501 agatatggga atttttttat tcctagtctc cttattttat ttggaaaatg ctataattat
    22561 ctagaaggag tgaagctatg aaccatttag atttatctca gcgcttagcc gcagtgggtg
    22621 actttgtccc tgaatccgct cgtttagcag acattggatc agatcacgct tacttacccg
    22681 ttgcattgat attaagagga aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc
    22741 cttatgaatc agcacgtaag caagtcaaaa aagatggatt agaagaccgt atcaccgtac
    22801 gccttgcaaa tggtctagat gctatccagc cacaagatgg tattacagct gtcacgattg
    22861 cgggtatggg aggttcactg atccgagata tcctagaagc tggcaagttg aatcaacgct
    22921 taagcggaca agaacgtttg atccttcagc caaacatcgg agagcgtaca ttacgatctt
    22981 ggctgcaaga aaatcagtat aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa
    23041 tatatgaaat cattgtagcc gaaaaagaac aaaaaccagt tacttacaat gaaatggagc
    23101 tgatgttcgg gccatttctg cttgaagaga aaagtgagat cttcaaatca aaatggacaa
    23161 gagagcttca acaaagaaaa gctatccgtg aacagctcaa acaagcagct aatcaagagg
    23221 cacgtcaaaa acaaatcgag caggaaatcc aaatgattga ggaggtttta aagccatgac
    23281 aatcagcgga cgcactttca tccaaaagtt tgaaagctat tgtcctcagt ggctagctga
    23341 agaaggagac ccagtcggac tgcatatcgg aacattggat aagccaatcc aacgagtaat
    23401 gatgacatta gacgttcgtc cagaagtggt cgaagaagca atcaagaaaa aaatcgattt
    23461 gctgattgct aaacatcctc caatctttcg tcctgtaaaa cggctggtta ctgatcagcc
    23521 acaagaaaaa atgtatgcag atttgttgaa acatgatatt gccgtctatg cagcacacac
    23581 gaacatggat attatttggg atggactgaa tgactggttt tgtgaacttc tcggtattga
    23641 agtggagagt tatttagtaa agactcatga gattcattat aaaaaactgg ccgtttatgt
    23701 acctgtggat catgcacaga aaatgcgtga agtattagct gcagctggtg ctggtacaca
    23761 aggtgattat accggtacaa gttttacctc gattggacac ggtcgattta ctccggaagc
    23821 tggtgcacaa ccagcgattg ggaaagtagg gaagacagaa caagttcaag aggcaaaagt
    23881 tgaggttatt ttgccggaaa ctatcgaaaa acaagtgatt caagccatgc ggtcagctca
    23941 tccttatgag gaacctgctt atgatctttt cgctatagat gaaccagtcg aaatgtttgg
    24001 gttaggacga gtaggtgaat tgccgcaaga aatttctata gaggcatttg tagaacaagt
    24061 aaaagaagcg ttccaactag atggactgag aatagtccag ccaaaaaatg caaaatcctc
    24121 tgtgaagcgc attgcgattt gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc
    24181 ccaaagagca gatgtctata tcacgggaga tatctattat catacggctc atgatatgca
    24241 aagcgctggg ttgattgcaa ttgatcccgg tcattatatt gaatcgttat gcaagcaacg
    24301 atttatcgag aaatttgaat cttggaaaca agaagaaaat tgggatatta actttttcgt
    24361 ttctgaaaca aatacaaacc catttcaatt taattaaaag gagagaatac tatgtacgaa
    24421 aatcttttgc cacgtttttt acgatatgtc aaaacagaaa cacgctccga tgcaacaagc
    24481 aaaacgactc catcgactca gacacaagtt gcttttgcgc aagaattgaa aaaagaatta
    24541 gaagagttag gcttagctga tgttttctat aatgaagata acggattcgt tatgggaaca
    24601 ttaccaagca atgttgatca tgaagtgcgt tccattggat ttatttccca catggataca
    24661 gcagatttta atgcagtagg agtcaatcca caaatcattg aaaactatga tggcgagtct
    24721 acgatcgtac tagatgcgga agaacgctat acattgaata caaaagattt tccaaatttg
    24781 aaaaattatg aaggccacac attgatcaca acagatggca gtacattact gggagcagat
    24841 gacaaatcag ggattgcgga aatcatgact gcaatggaga tcttgatcaa taatccttct
    24901 atccctcacg gtaccatcaa agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg
    24961 gataaattcg atgttgagca attcgatgta gattttgctt atacagtcga tggtggtcct
    25021 ttaggtgagt tgcagtatga aacgtttagt gctgctcaag caaatattac gatccaaggg
    25081 aaaaatgtac atccagggac ggcaaaagat acaatgatca atgctctaca gttagcaatc
    25141 gattttcata atcagcttcc agccgatgaa gttccagaaa aaacggaagg atacgaaggt
    25201 ttcttccatt tgatggcatt ggatgggaat cctgaagaag cttctatgag ttatatcatt
    25261 cgcgatcatg atcgtgaaaa attcgaagca cgcaaagcac aaatcacaca tatccaagaa
    25321 gagttgaatc agcgatttga tcaagaacga atcaaagtag atatgtatga tcaatattac
    25381 aacatgaaag aaatcattga aaaagatatg agcatcgtgg aattagcgaa acaagcaatg
    25441 atcgagcttg gtatctctcc agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa
    25501 atctcttaca tgggtattcc gacgccaaat ctatttgccg gaggagaaaa tatgcacgga
    25561 cgattcgaat ttgtttcttt acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt
    25621 gcagaattga acacaaagag ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc
    25681 ccaatgatgg aatgtcatct gtctaaggct aaagggcagg acaatggaat tggttgtccc
    25741 gccctctttt ttgaagagta aataggaggt aaagaatgga aaagctgcta gttttttata
    25801 acaaaagttc agggaaagat gagggtgaga aattagcacg ctggtttcaa gattatgtaa
    25861 aagaaaagca gccagaatta tatgtagaac tagctgaaac tggcccatcg attgatgatg
    25921 cggaattagt caagaaagca gaagccatag aagctgatac tttagtaatt ataggtggag
    25981 acggcacgat tcatcatatc gtccaagcgt tccaaaaaac attatctcaa taccatgtcg
    26041 gattacttcc aggtggaact gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa
    26101 aagaagaagc tgctgacgtg atcctggaag gcaatgtacg gaaaatagac tatggtcaag
    26161 tcaatgatga agtcattatc tctacactga caatcggaat cttagcagat acagcagttt
    26221 ggatcagtca agaagaaaaa cagcgttacg gctcgtggat ctttatcaag aagtttttta
    26281 agttattgct gaagaagaaa aaatatcatt tggatttaga aacagacgag gagcattgga
    26341 aaggaaaatc ccaactgttg acagtcacga tgtctaactc cgtgggcggt tttacaaatt
    26401 ttgacgactc agcgacccta gatgatggaa aatttcatgt aacgatcgta ccttccttag
    26461 atattttccg atttattttt tatttgccta gaatcatgag aggcaaattc tatacgatcc
    26521 ccggcattac ttatttcaca gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg
    26581 gcactcgaac ggatggagac acgacagatg atcttccagt tgaattatct gttgtatcaa
    26641 aaggattaac agtttattca ccaaagaaag aagaaaaagc ttagaaatag tagttaacag
    26701 aaagttggaa gaagagtaat ggatgtattg ttacacaatc ctttgtggtt ttaccttttc
    26761 attgtgaatt tttacttatt ttgtctgatg gggtatgata aacatcaggc tagaactagc
    26821 ggctggcgtg ttcctgagaa taatttgata tttgtcagcc tgttaggcgg tggtttggga
    26881 ggattgatct ctcgaaaagt tttccatcat aaaactcgga aaaagaagtt tactatagga
    26941 ttcgctgcag gtgtacttgt tgatttgatt ctcatttatt ttttccatta aggagcagga
    27001 gatgtcgtct aaaggaacga aaactaaaat cattctgaat ctgcttttgc tgattgtgat
    27061 ttttgccatt atttattatt taatcaatca atcatttgca gatatctttg ccgaattgct
    27121 gtctaccagt gttgttgttt tggcagcggc tgttatatta gggacagttt accaaatcgc
    27181 tgaagggcgc tcaatcaaag aaattgctga acctttcgcg ccgaatttta caacgaaaga
    27241 tgggttcttg acctctagct atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac
    27301 gctgatctcg gagatttatt tttataacaa aaaaggaatg aagctatccc agggagcagg
    27361 ggtgacagca ctccatatga ttatgtacaa actggcagtg atcacttatg cattagctgg
    27421 cttggtcatc caattctctc ttttttattc taatggtcca tcaatgatct ggtttatcat
    27481 cgctggtatc cttttgacga tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt
    27541 gaatgttcaa atattttttg tcagcttatc gaataagtta tttaaaagtg agcgtttgcg
    27601 gaaatgggtg gatacatgca atcagcagat ctactcttta agggaaacgg tccaaaccat
    27661 tatccaagac aggactgcgc tattgcggat ttatctatgg aatatgttaa aacttgtatt
    27721 ttggtatgta cttccttatt tgattctgat tgaaaaccat cctaaaatag attttctact
    27781 gaccatttcg tttatcagct ttgcagtagt gctttcaggg gttattccta cgcctgctgg
    27841 tattggttcc tttgaatttg tctatttgtt attattcaga ccagttgttg gaacagttga
    27901 cgcagtttct tcgcttttac tgtatcgttt cagttccttt atcttgccat ttctctatgg
    27961 atttgtgtat gtgattgctg aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa
    28021 aaaagagaat aaagaaaatc aagggtaggt cttttataag atctaccctt tttatcaaat
    28081 caaagaaaaa cgttatggat aagataat
//
LOCUS       DAZKCZ010000028        27680 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000028 DAZKCZ010000000
VERSION     DAZKCZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.028"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            250..2016
                     /locus_tag="ACG8KN_001109"
     CDS             250..2016
                     /locus_tag="ACG8KN_001109"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFP9188180.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2118..3377
                     /locus_tag="ACG8KN_001110"
     CDS             2118..3377
                     /locus_tag="ACG8KN_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9188181.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3524..4825
                     /locus_tag="ACG8KN_001111"
     CDS             3524..4825
                     /locus_tag="ACG8KN_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9188182.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4828..5679
                     /locus_tag="ACG8KN_001112"
     CDS             4828..5679
                     /locus_tag="ACG8KN_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9188183.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5692..6507
                     /locus_tag="ACG8KN_001113"
     CDS             5692..6507
                     /locus_tag="ACG8KN_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFP9188184.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6658..7824
                     /locus_tag="ACG8KN_001114"
     CDS             6658..7824
                     /locus_tag="ACG8KN_001114"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9188185.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8054..8701
                     /locus_tag="ACG8KN_001115"
     CDS             8054..8701
                     /locus_tag="ACG8KN_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9188186.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8785..9729
                     /locus_tag="ACG8KN_001116"
     CDS             8785..9729
                     /locus_tag="ACG8KN_001116"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFP9188187.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9864..11315
                     /locus_tag="ACG8KN_001117"
     CDS             9864..11315
                     /locus_tag="ACG8KN_001117"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFP9188188.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11336..12171
                     /locus_tag="ACG8KN_001118"
                     /pseudo
     CDS             11336..12171
                     /locus_tag="ACG8KN_001118"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            12171..12830
                     /locus_tag="ACG8KN_001119"
     CDS             12171..12830
                     /locus_tag="ACG8KN_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9188189.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12956..13645
                     /locus_tag="ACG8KN_001120"
     CDS             12956..13645
                     /locus_tag="ACG8KN_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188190.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13651..14607
                     /locus_tag="ACG8KN_001121"
     CDS             13651..14607
                     /locus_tag="ACG8KN_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFP9188191.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14651..15163
                     /locus_tag="ACG8KN_001122"
     CDS             14651..15163
                     /locus_tag="ACG8KN_001122"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9188192.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15419..15967)
                     /locus_tag="ACG8KN_001123"
     CDS             complement(15419..15967)
                     /locus_tag="ACG8KN_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9188193.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16056..17237)
                     /locus_tag="ACG8KN_001124"
     CDS             complement(16056..17237)
                     /locus_tag="ACG8KN_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFP9188194.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17473..18981)
                     /locus_tag="ACG8KN_001125"
     CDS             complement(17473..18981)
                     /locus_tag="ACG8KN_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFP9188195.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19140..19331
                     /locus_tag="ACG8KN_001126"
     CDS             19140..19331
                     /locus_tag="ACG8KN_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188196.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19534..20451
                     /locus_tag="ACG8KN_001127"
     CDS             19534..20451
                     /locus_tag="ACG8KN_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFP9188197.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20444..21517
                     /locus_tag="ACG8KN_001128"
     CDS             20444..21517
                     /locus_tag="ACG8KN_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188198.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21665..22183
                     /locus_tag="ACG8KN_001129"
     CDS             21665..22183
                     /locus_tag="ACG8KN_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188199.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22164..22556
                     /locus_tag="ACG8KN_001130"
     CDS             22164..22556
                     /locus_tag="ACG8KN_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFP9188200.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22632..23567
                     /locus_tag="ACG8KN_001131"
     CDS             22632..23567
                     /locus_tag="ACG8KN_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9188201.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23700..24479
                     /locus_tag="ACG8KN_001132"
     CDS             23700..24479
                     /locus_tag="ACG8KN_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9188202.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24472..24789
                     /locus_tag="ACG8KN_001133"
     CDS             24472..24789
                     /locus_tag="ACG8KN_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9188203.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25033..26643
                     /locus_tag="ACG8KN_001134"
     CDS             25033..26643
                     /locus_tag="ACG8KN_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9188204.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26800..27648
                     /locus_tag="ACG8KN_001135"
     CDS             26800..27648
                     /locus_tag="ACG8KN_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9188205.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8966 a         4891 c         5554 g         8269 t
ORIGIN      
        1 aaaaactgtt aaaaaacttt tactttagaa aattggagaa attgatcgct taagataaat
       61 tcaggacact aaatagtaca ggaagtagat taaaactttt aaaagtttta agtctatcta
      121 tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac tgttaccgat aacatttttg
      181 aaagcgttgt cttttgtttc tctatcactt atagtgatga cgtcatatta aaacacaagg
      241 aggaaatcaa tggatacagc tgcaatttat catcgaccgg aaagtgaata tgcatttcta
      301 tatacgaata caagatttcg tattcggctt cgtactagga aaaatgatat caaaaaagta
      361 tatgtgcttt gtggagatcc atataccata acaactgaaa aatggtatca aaaacaacga
      421 ccaatgaaaa aatgtctaag taccaatgtt catgattatt gggaaattga agtgacgagc
      481 gaaacaaggc gcttacagta tgcgttccat gttgttgggg aagataacac ggactgtttt
      541 tacggtgatc aaggaatttt tccttatcga gaaaatgtga ttactgaacc aaatttttat
      601 tttcgaatac cttattttca tcaaatcgat cgattcacta ttccagaatg ggtaaaagaa
      661 acagtatggt atcaaatttt tcccgaaaga tttgcaaacg gggacaaagc aaatgacccc
      721 gaacacactt taccttgggg aagtaaagat ccagaccgag aagacttctt cgggggagat
      781 ttgcaaggtg tgagggatca tttggattac ttgacggacc ttggcgtcaa tggcatctat
      841 ttttgtccca tatttaaagc cacatccaat cataaatacg atacgattga ttattatgag
      901 gtggatcccg cttttggcga taaaaaactt ttaaaaaact tgattgatga agcacataaa
      961 agagggatac gtatcatgtt cgatgctgta ttcaatcata tgggtgtcca ttcaccacag
     1021 tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata aagactggtt ccacatccgt
     1081 tttttcccag tagattctta tcagatgact gacttaccag aaactgctga aaatatccca
     1141 tatgatactt ttgcttttac tccattcatg ccgaaactaa atacagcaaa tccagaagtg
     1201 caaaaatatt tgctggatat cgcaacttat tggataaaag aattcgatat tgatggatgg
     1261 cgtcttgatg ttgctaatga agtcgatcat catttttgga aaaaattcag agaagcagtt
     1321 acagaaatca aaccagacat ctatatcctt ggagagattt ggcattcctc tcaagcttgg
     1381 ctgcaagggg atgaattcca tgcagtgatg aattatgcat tcacagattc gattaaagat
     1441 tattttgcaa aaaagaaaat cacagcttca caaatggtca gtggaatgaa tcatcagcaa
     1501 atgctttaca gagatcaggt gaacgaagga acattcaact tattagattc tcatgatact
     1561 gccaggatct tgaccctttg ccaaggtaac aaagaattga tgaagtctgt gatggccttt
     1621 atgtttcttc aaaaagggtc tccttgtatc tattacggga cagaaatagg gatgactggt
     1681 gaagatgacc cggattgccg caaatgtatg atatgggaga aggaggaaca agatctagag
     1741 ctattcggtt ttataaaaga acttgtttct ctaaggaaac agttgtctaa gattatttct
     1801 gaaggttcta ctcagtggct aatagtatat gatcgtgaag ataagctcta tttcacacgg
     1861 gaactagagg gacagatcat ctatgtttac tttaatcagt caaaagaacc ttgggtagta
     1921 gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt tgttagaaga cgggaaagca
     1981 gagatacgac aatacggatt tttgatcatg tgttgaaaac tgttaccggt aacaacaaga
     2041 aagcgtttgc aaatgaagat ttgtgcccat tataataagg gtgtaacagc acgttgaata
     2101 cacaggagga aatgaagatg aaaaaaagaa gtgtgaaaca gttgggttta gggttaatct
     2161 ctttaggaat cttaggcgga ttagcggctt gtggcaatgg taactcttca gcagaagggg
     2221 aagccgatga aggaaaaacg ttaacagttt ccgttgattc tggatataaa gattatataa
     2281 acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa aatcaagcta gtagaaaagg
     2341 acatgttcga tcagttggaa tcattagcgt tagatgggcc tgctggaaag ggacctgacg
     2401 ttatgatggc agcatatgac cggatcggtg cgttaggaca gcaaggacat ctggcggaag
     2461 taaaactggg aaatgaagca gcttacgatg aaacagacaa agcccaagtg acttatgatg
     2521 gaaaaatcta cggagaacct gcagtaatcg agacccttgt tttgtactat aacaaagatt
     2581 tagtcgatac agcaccatca acgtttaaag atttggagaa tctatccaaa gacagtcgtt
     2641 ttgcttttga gtcagaagca ggaaaaaata caggattttt agctaaatgg actgactttt
     2701 attattccta tggtttaatt gcaggttacg gtggctatgt atttggtgat gatggcacaa
     2761 atccttcaga tatcggtttg aataatgcag gtgctgtaga aggaatctct tatgcaacag
     2821 attggttcaa aaatgtttgg ccaaagggaa tgcaagacat aaaaagtgca ggagacttcg
     2881 ccagccagca attcatgtcc aataaaactg cagcaatcat tgacggtcca tggcaagcgc
     2941 aaacatacaa agaaaacaat atcaattatg gtgtagccaa aattcctact ctaaataatg
     3001 gacagcctta tcaaccgttt ggcggaggaa aaggatgggt agtcagcaat tatgctaaaa
     3061 acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa tcaggaaaat caagaaaaat
     3121 tctttgaaat ggtaaatgaa atccctgcaa accaacaagc tagggaaaca gcaaaggcta
     3181 aaaacgatga attaacaaca gctgtgattg aacaatatga aacagctcaa gcgatgccaa
     3241 acatcccaga aatgggcgaa gtttggacag gtgcagaaaa cttgatgttc gatgcagcat
     3301 caggttcaaa aacaccaaaa gaatccgcag atgaagcagt aaaaacaatc agtgaagcca
     3361 tagaacaaaa atactgatta aaatgataac cgtccatttt tataaaaaat ggaacaggtg
     3421 cttcagataa aagtggcaag agcgagatgg aaaattatct tgctcttgtt gccattatct
     3481 taaaagcagt ttgcggttta gcaagtttag agggggcatt cagatgcagc aagataaaac
     3541 aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt attccaggga tggggcagtt
     3601 tgtaaatggc caagctttta aaggtatcgt ttttttcgcc atattcgtcc tattcattgg
     3661 tcagatgctt ctgggcggat ttcatgcatt ggtaggattg gttactttgg gaagtatacc
     3721 aatggaagat cattcattat ttattttgat tgaaggcact ttgcaattca tcattacaat
     3781 cattttcttg attttttacg gtttgaatct tttagacgct tatcgagtag cgaagatgcg
     3841 tcaagagggc aaaaaaatca atcggacgat gaaggaagtc atacataata taggtgatga
     3901 aggttttcct tatctattga cacttcccgc ttatcttctc atgattttta cgatcatttt
     3961 cccagtccta gttacattgt tcactgcatt tactaattat gacttcaatc atattccacc
     4021 agccagttta attgattggg taggggctaa aaactttttt agtatcttct ttttaagctc
     4081 atatcgtaat actttttttg cagtcttttc gtggaccgtg atttggactt tatgtgcaac
     4141 aactttgcaa atcacccttg gtattctcac agcggttgtt gctcaccaga aatttattaa
     4201 attcaaacgt attttcggta tcattttctt attaccatgg gcggtcccag cttttattac
     4261 tatcatgagt ttttcgaata tattcaacga tagcgtaggt gccatcaata ctcaagtgat
     4321 tcctttcata aaccagcttc cttttgtaga tatcagtgca atctcttgga aaacggaccc
     4381 gaactggacg aaagtagcaa ttatcttagt gcagggctgg ttaggatttc cctatatcta
     4441 tgtgatggtt acaggaatct tacaatcgat ttcagaagac ttatatgaag cagcaaaaat
     4501 cgacggagca aatgcatggc agcgcttttc aaacatcacg cttcctgctg tcttcctagt
     4561 ggcagcgcca acatttatta cacagtatac ggggaatttc aataatttct ccatgatcta
     4621 tctgtttaac aatggtggtc ccggaagcgt aggcggagga gctggttcta ctgatattct
     4681 tatttcctgg atctataaac ttacgactgg aacttctccg cagtattcta tggcttcagc
     4741 aatcacgttg attatctccg tcatcgttat ctgtgtgtca ttgctggtat ttaaaaagac
     4801 aaatgcgttc aatatggggg attaacaatg aaaaaatttc attcgagtgc tcgattccaa
     4861 aaacgacttt ctcacttttt tacttacttt tttcttttaa tactgtcagt cattatcatc
     4921 tatccgcttt tgatcacagc aagtactgcc ttcaagtcag gaaatattgc agcttttagt
     4981 ttagatcttt caagtgactg gactttagga aactttactc ggctattcag tgaaacactt
     5041 tacgggactt ggtataaaaa tacattgatc atttctgtcc taacaatgat cgtacaagtg
     5101 actgtagtaa ctttggcggg ctatacttat agtcgctatc gttttatcgg ccgcaaatca
     5161 agtttgatgt tctttttagt gattcagatg gtaccgacta tggcagcatt gactgccttt
     5221 tatgtcatgg ctttcttatt agaagcgctc gataagtact ggttcttgac aatgatctat
     5281 ataggcggtg gaattccaat gaatacttgg ctgatgaaag gatactttga cactgtacca
     5341 atagatttag atgaatctgc taaacttgat ggggcagggc atttcaggat ttttttccag
     5401 attgtattac cgcttgtcaa accgatgctc gccgttcaag cattatgggc ttttatggga
     5461 ccttttgggg atttcatgtt agctaagttc ctgcttcgaa cacctgaaaa tcagactgtt
     5521 gctgtcggct tacaaacatt tatttctgat gtgacaaacc aaagagtctc tctttttgct
     5581 gctggggcaa ttttgattgc actaccgatt tgtctattat tcttcttttt acaaaagaac
     5641 tttgttaccg gcctgacatc tggcggaaca aaaggatagg agcagagtat catgcaaaca
     5701 tttccgttaa actatttttc cgcattacgt acgccaactc aagtgtttgc gggacgcaaa
     5761 ctattatcat ggcctaaatt tttcttgata tttgtatttt tagtttcctt gatggtcatg
     5821 cctgtgactc ttttttatgc caatcagatc caagcgattc cattagaaca atttctatcc
     5881 gttcattcgc tgatcgatga acaaggaacc caaaaatttt ctgaacttga attatctgaa
     5941 actgggttac aagcatcgca acaaacgata gctgtgaccc ctgaaatttt ggtaggtgtg
     6001 tctttatctg agaaacaaca atccgatcat ggaactttta ttgattttga gaaggaacaa
     6061 tgggtgatcc aacaaaagga taaatctggt atacgccgct atacgatgaa ttatagccca
     6121 tctttccaac cagattctgt acgtacgccc gaagatttcc aacgattttt agaaagagaa
     6181 ttttatgcca gtaatcgtcc aacaatcatt ttatcttata gtttaagtct tggtcttgtc
     6241 ctatttgtca tgacgagtct cattttattc ggggcttcct ttttcttgtg gatgacaaga
     6301 aaaagccaac tttcatccat tcatacattc aaagagagtg caaatttgat gctgaatgtt
     6361 atgggaatag gttccatggc agcagcagtt gtagggttta tccactttga ctttatcttg
     6421 atgctaagtg tccaaacaac gataactgtc ttgctgcttt tatgggtttt tgtcaaaaca
     6481 agattcaaag ataagcgtgt cgaatgacaa gtttaagcga tttatgcgac aatgaatgtg
     6541 aaaaaaatga aaaataaagt agtttttgaa aaatttctga aaaaattaaa cttcttagtt
     6601 gattcttttc ttttttagta ttaacgtaat agggtagttt ttttaaggag gaaaatcatg
     6661 ttagagaaaa ctttatacaa tcaattattt tcacgttctt tctcgctccc tgtcgaagta
     6721 aattattggg acggtacaac gaaacaatac ggagatacgg acactcctcc ccaaatcaaa
     6781 atcaatatcc atgaagaaat cccaatgaaa gagattacaa gtaacgcttc tttggcacta
     6841 ggagaagcct atatggatca tcgaatcgag atcgagggaa gtatccaagc attgatcaac
     6901 gatgcttatc agcaagcaaa cagcttttta cgaggaaaag attatctaaa atggctgcca
     6961 aagaaagaaa aacatacgaa acaaaaaaac aaagaagata tccatgcgca ttatgactta
     7021 ggtaacgact tttataaact ttggctagat ccaactttga cttattcttg tgcttatttc
     7081 aatacacctg aagatacgtt ggaacaagca caaatcaata aagtccatca tatcttagac
     7141 aaactcttta tcaaagaagg cgaaacgttg cttgatatcg gttgtggctg gggaacattg
     7201 atgttcactg ctaccaaaga gtataatgtc aaggcaactg ggatcacact aagtgaagaa
     7261 caatacgatt tgattaccaa aagaataaaa gaagaacatt tagaagataa atgccgtgta
     7321 ttattaatgg attaccgcga actaaaaggc gaaacgtttg atcatatcac aagtgtaggt
     7381 atgttcgaac atgtaggttc tgaaaatctt gaaggttatt tcaaagtggt aaaagattta
     7441 ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc gacaacaagg cggtgcgaca
     7501 aatgcttgga tcaataaata tattttccct ggcggttatg tccctggtgt tactgaatta
     7561 gttgataata ttacaaaaaa cgatttgcaa ctgattgatt tggaaagctt acgtcgtgac
     7621 taccaattga cattagaaca ttggacaaag aattttcatc aagtccaaga tcaagtcacg
     7681 aaagaaatgg gtgattcctt ctatcgaatg tgggatctat atttacaagc ttgtgcagct
     7741 tcatttgaat caagtaatat cgatgtgatt caatatttgc ttgttcaacc tggaaacaat
     7801 gacatcccaa tgcatcgtgc ataattgagg caaaagtact gtttaaagac tatttaaaaa
     7861 gaagctaagt tttatgatgc tccagaagtg aacttttttg tctaacttct aggattgtct
     7921 ctaaagctta gtttcttttt ctttatattt tcataataaa agtttggttc tttaattttt
     7981 aaacatgaca gaaaattttt tcttcgttat acttgtaata gaatactatt ttataacaaa
     8041 ggggatcgaa tcaatgaaaa aaactgttat ttatactagt ttatttgcag ctttgttttt
     8101 agctgcttgt caatcaaata ccaatcaagg cacatctggg aatacgtctt ccagtagcga
     8161 acagcgctct tcacaggtat cggactcctc aacaaaagca agcacatctt cgtctagcca
     8221 gacaaaaagt tctagtgaag caagtactca gtcagatatc aaagtgtctg cagctcgtgc
     8281 gatcgaattg ttccaagata aatacccaga tactgcaatt actagtttgg aattagatag
     8341 tgattgggga tcttacttct acaagataga aggtgtcgat gatcaaaaag aatacgaagc
     8401 aagaattgat gctcaaaatg aaaaaatgga gacagagaat ccagaaactc tagatcgaga
     8461 tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat gttgataaat tgatcacgat
     8521 tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc ggtacggcaa cagattggga
     8581 tttggataaa gatttaggta ctacttattg ggaagtcaaa gtaaaagacg gcaatcaaag
     8641 tacatcagtc aagatcaata gccaaacagg cgaagtactg tcaactgaac aagatgatta
     8701 agaatttctt cggaaaacaa gagttgattt ttcttttagt catgatacac tagacaaaat
     8761 aacttgtttg aaggagcgat ttacttgagt ttagaaacga cctttgccaa tcatatcttt
     8821 gccaatcctt tgatgaatgc atctggtgtc cactgcatga ctacccagga attagatgaa
     8881 ctggctcatt cagaagcagg agcatttatc actaagagct gtacgatcaa tgaacgaaaa
     8941 ggaaatcccg aaccacgcta ttttgatgtt cctttaggga gtatcaattc tatgggactt
     9001 ccaaacttag gtttttctta ttatctagag tatgctcttg cgtatgaaaa agtgcaggaa
     9061 aaccaaaatc aaccgttatt tttttccatt gccgggatga gtgtccagga aaacttggaa
     9121 atgcttgaga aaatagaaaa aagtgggttt aatgggatca ctgaattaaa tttatcttgt
     9181 cccaatgtgc ctggaaaacc acaactagct tacgattttg aggccactta tgaaacactg
     9241 aaagaagtat tcagcatctt ttctaagccg ctaggaatca aattgcctcc ttattttgat
     9301 tttgctcatt ttgatcagat ggctgatatc ttgaatcagt ttccattgac ctatgtcaat
     9361 gctatcaaca gtgtgggcaa tggtttgtat atcgatacag aacaagaagc agtagtcatc
     9421 aaaccaaaag aagggttcgg cggcattggc ggagagtata tcaagccgac tgctttggcg
     9481 aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc aaatcattgg tacaggtggg
     9541 attcggacag gacaagatgc atttgaacat ctgctatgtg gcgcaagcat gttacagatt
     9601 ggtacagagc ttcataaaga agggccggaa attttttcta gaatcataaa agaattaacg
     9661 cagattatgt cagaaaaagg atatacgtca attgacgaat ttaaaggcaa attacgcacc
     9721 atttcatagc aaagagacga aggtcatgat tgatcttcgt ctttttttat aaaaaacact
     9781 cgtctgttca ttcctgtgca agcgtactca ttgtatgtta gacgaacttg tgggaaaata
     9841 acagaggcaa aaaggaggat aatatgaatg atgcaacaga attagcaaaa gagcttaaag
     9901 aaaaagaatt tagttttcaa gaactagtta cggatataca gaaaaaaata aagaagcaaa
     9961 atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga gagtaattcc cacgagctat
    10021 tgattcccca gtcagaaaaa gggccattgg ctggtatccc atttccttta aaaatgctcg
    10081 gtcaagaaaa aaagggatgg ctagctactt caggatctcg tttatttgaa acacaccgag
    10141 cttctcatac atctaattat gtgcggcaag cagaagcgat aggtctagtt ccttttggac
    10201 aaaccaatgc gccagagttt ggtttcaaaa atattacaga cccagtcatc tatggcccag
    10261 caagaaaccc ttggaacctt gaatgtacac cgggcggatc aagtggagga gctgctgctg
    10321 cagtttcatc aggaatcgtt cctttagctg gtgctagtga tggcggtggt tctattagaa
    10381 tacccgcttc gttttgcgga ttgattgggc tgaaaccttc gcgagggacg atgccagttg
    10441 gtccatatgc ttggagagga tggcaaggag ctgctattga tttcggcttg acagtttcta
    10501 tgagagacac ggaggcttta ttttatggga tgagaagtat ccattccggt gcgccatatc
    10561 aagtatcgcc agttgagtgg caaacacatc caagaaaaaa acggttgaag atagcttttt
    10621 gtgttgattc accgatccac tcacagatta gtgaagaggc agtacttgct gtaaaaaaag
    10681 ctgtagattt ccttggaaaa gaagggcatg atttgacaga gattccttac ccactagatg
    10741 gtcgtaaact gatcacgtct tattatcaga tgaatggcgc agaaacagca gcaatgatcc
    10801 gctcgattga aacaggcatc cagcgagctg tcagaaaaga agaacttgaa cccatttctt
    10861 ggactttgtt ccaatatggt cagaagctct ctgccgctac ttacatcgat tctctgcatg
    10921 tatgggatga agcagcaatt gtcatggaaa acctacatca agaatacgat ctattccttt
    10981 caccaacgac tgcatttacc gcccctaaaa tcagcgaaga tttgcaaagt gatgcgataa
    11041 gagcacagat ggaacacatc agtgattgtt ctgaagtaga aggtttagac gttgtttctt
    11101 ccatgtttga ggagagcttg aagatcacac cttatacaca gttggctaat ctgactggac
    11161 aaccagcaat cagcttgcct actcatttga caaaagaaaa catgcctcta ggaatccaat
    11221 ttatggctgc cagaggaaga gaagatttgc tatttcaaat cggctatcta tttgaagcaa
    11281 atcatcagtt ccatttacca aatccatgga aatagcagaa acaaggagaa gaagaatgaa
    11341 gaaaaaaaga ttgtttgctc taataactag tgcctttttt attttaagcg cttgcggtac
    11401 agctgccgct aatcaggaag ataacagctg gagtaatgta gaaaaaaaga aagaattagt
    11461 agtagcgact tctggcacac tgtttcccgc atcttattat aatgatcaaa atgaattagt
    11521 tggttatgat gtagatttgg caaaagaggt ggctaaacgg ttaaatgtca aaatctcctt
    11581 taaagaatac aacgtggatg gacaaatcac ttctgtttca aaaagagaat cagattttgc
    11641 tgcgaacgat tttggtatca caaaagaaag agaaaaaaat tctccctttc agagactttc
    11701 aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt
    11761 gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg
    11821 gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc
    11881 gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc
    11941 gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac
    12001 gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta
    12061 ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact
    12121 gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc
    12181 ctctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat
    12241 acacattagg gattggcttc gtgtcgtttc ttttaggaaa tttggtaggg ttgcttttga
    12301 cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt
    12361 tctttagagg cattcccgca ctagtcctgc tgtttctgtt atatttcggg ttaccttatc
    12421 aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg
    12481 gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag
    12541 cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa
    12601 tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag
    12661 caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg
    12721 attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg
    12781 aaaaagtgca acagtattta gaaaaacgtt ctatccatca gtcaatctga aaaatagaaa
    12841 tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat
    12901 tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa
    12961 gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg
    13021 cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg
    13081 cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc
    13141 agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc
    13201 aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta
    13261 tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa
    13321 agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc
    13381 tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaaa
    13441 agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga
    13501 acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga
    13561 agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg
    13621 gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa
    13681 tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa
    13741 ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa
    13801 aaaaagcacc atccaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc
    13861 gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc
    13921 ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat
    13981 ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa
    14041 tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc
    14101 cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt
    14161 tttagtgatg actatctttt tttccacgca gacggatata ttggattttc tgatttcacg
    14221 atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta
    14281 acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac
    14341 tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc
    14401 atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac
    14461 cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg
    14521 gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt
    14581 caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag
    14641 aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat
    14701 cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca
    14761 gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat
    14821 agtggaagat caaattgctg gaacagctgc attagtctca ggcgtggatc ccgtttacac
    14881 aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc
    14941 tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac
    15001 gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc
    15061 gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt
    15121 tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag
    15181 caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta
    15241 acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt
    15301 gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt
    15361 cgtgacatag cttttgtcac gatctctcgg cttatcctac gagtattatt cgatacattt
    15421 atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca
    15481 cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa
    15541 ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca
    15601 atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata
    15661 aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt
    15721 taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact
    15781 catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat
    15841 ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa
    15901 aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat
    15961 aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg aaaatatttc
    16021 caaagctttt aaaagtaact ttgctttttt gtattttata ccagcaagcc agtcacaata
    16081 gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca
    16141 agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc gatagaagag
    16201 aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc agtaaaatgg
    16261 aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag tttagtcgcc
    16321 atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa ggcaagaggg
    16381 gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg gaagatagca
    16441 ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt gatcgccaca
    16501 tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc tttgttttct
    16561 tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat tgaagccaga
    16621 atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga atcgatcagt
    16681 gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac actcatggtg
    16741 aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa aacttccgat
    16801 tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat cccgttgatt
    16861 ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat atagcgtaga
    16921 ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca
    16981 cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga
    17041 agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt tgtacgtaat
    17101 aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt caatttgatt
    17161 gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa accaataata
    17221 cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt tacctgtttt
    17281 gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg aaacgcattc
    17341 attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt
    17401 aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc aacatttata
    17461 agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat
    17521 atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg
    17581 ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt
    17641 tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga
    17701 atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg
    17761 aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc
    17821 atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat
    17881 cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc
    17941 agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat
    18001 tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata
    18061 acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa
    18121 tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat
    18181 tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc
    18241 tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac
    18301 tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa
    18361 agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt
    18421 caagcaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt
    18481 agttgtttca atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt
    18541 gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt
    18601 attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat
    18661 taataagcca taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat
    18721 cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat
    18781 ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg
    18841 aagtacacgt ttcgcatgct tgtcgtcaac taacttccca ccaaattcta aatataattt
    18901 gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga
    18961 atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt
    19021 tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa
    19081 accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca
    19141 tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg
    19201 gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg
    19261 aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc
    19321 ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa
    19381 gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat
    19441 tgcccgctct tttttataac aattttttac agaaaatggt actattgaag tattaataag
    19501 tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat
    19561 tatgagccag acgaactaga ggagacgaac gcggatcagg atacaggaca agaatcgtct
    19621 tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta
    19681 caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat
    19741 ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg
    19801 ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa
    19861 aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg
    19921 gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg
    19981 gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca
    20041 aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat
    20101 cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa
    20161 gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt
    20221 ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca
    20281 agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat
    20341 ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc
    20401 aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga
    20461 agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt
    20521 ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac
    20581 aaaaatacat gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca
    20641 tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga
    20701 tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga
    20761 tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga
    20821 agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact
    20881 gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac
    20941 aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt
    21001 attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc
    21061 agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc
    21121 agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt
    21181 attagcccat gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa
    21241 gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat
    21301 ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta
    21361 tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac
    21421 agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta
    21481 cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat
    21541 gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa
    21601 gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt
    21661 aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg
    21721 taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat
    21781 agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag
    21841 cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt
    21901 tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa
    21961 gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac
    22021 gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt
    22081 gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt
    22141 ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg
    22201 tcagtatact cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa
    22261 aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact
    22321 tttcggaaaa agaagtggtc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa
    22381 attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg
    22441 agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca
    22501 aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt
    22561 cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga
    22621 aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag
    22681 gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg
    22741 ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat
    22801 tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag
    22861 caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg
    22921 gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca
    22981 ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa
    23041 tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa
    23101 gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg
    23161 tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga
    23221 tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat
    23281 tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag
    23341 cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa
    23401 cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact
    23461 cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata
    23521 tatacattga aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat
    23581 gaattgaagc aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta
    23641 gcgaatttat gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa
    23701 tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta
    23761 taagctatat accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc
    23821 ctctcttatc aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg
    23881 catctatgct tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag
    23941 aattacgcta tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga
    24001 cgtttttggc tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa
    24061 agttttcaac tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa
    24121 tgactgcatg ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag
    24181 atgctgacct tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga
    24241 tgaaaagcgc actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca
    24301 tggtattatt ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg
    24361 gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta
    24421 aagatgtcaa tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa
    24481 ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc
    24541 ccgatgcttt attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat
    24601 gtaccggtgg cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat
    24661 ctggatattg cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac
    24721 aaaacacgtt cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct
    24781 atgctttaaa ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg
    24841 atctatccga actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa
    24901 aaaagattag tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat
    24961 ttgtccttct atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt
    25021 gagggaattt aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat
    25081 cttctatgta aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac
    25141 aatcaagatg gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg
    25201 ttcctgatgc ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc
    25261 acaaaagaag gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg
    25321 tttgtcatcg gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga
    25381 atcagtccga ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct
    25441 ttgattgcta tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg
    25501 cggccttatg gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct
    25561 ggtacttatt acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt
    25621 acagtcgctt ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc
    25681 agaaaagact tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa
    25741 aaccgatctg cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca
    25801 gtcattacta tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc
    25861 cgtccagaga tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca
    25921 aataaattga cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca
    25981 atattaagca cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt
    26041 ttttcgattg cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga
    26101 ccactataca cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct
    26161 ttgctagctt ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg
    26221 aatcaccatc gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc
    26281 caagtaatcg gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca
    26341 ttcggtattg tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct
    26401 ttagcaaaga tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca
    26461 gtatgttttg ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat
    26521 gcaatgattt attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa
    26581 ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa
    26641 taggttaact aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt
    26701 gtcgtttttc tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata
    26761 aggtctggaa aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca
    26821 gatagttcgg catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca
    26881 atccctttga ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa
    26941 tattatggac ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg
    27001 gaagttttgg gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc
    27061 catctttctt cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc
    27121 agcggagtaa cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg
    27181 gtagaagctg ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat
    27241 atcgatcgaa tccgagataa tacctatgct tatctgattg tagatgattt gaacaattta
    27301 gtacgaggag gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa
    27361 ccgatcctga cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa
    27421 aaagcatttg ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca
    27481 gtcaaacttt acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa
    27541 ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt
    27601 acacatttag gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc
    27661 aaaaaacata ccaaaaatat
//
LOCUS       DAZKCZ010000029        25952 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000029 DAZKCZ010000000
VERSION     DAZKCZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.029"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..700)
                     /locus_tag="ACG8KN_001136"
     CDS             complement(<1..700)
                     /locus_tag="ACG8KN_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFP9188206.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKE"
     gene            921..2135
                     /locus_tag="ACG8KN_001137"
     CDS             921..2135
                     /locus_tag="ACG8KN_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9188207.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2157..4172
                     /locus_tag="ACG8KN_001138"
     CDS             2157..4172
                     /locus_tag="ACG8KN_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HFP9188208.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4255..5628)
                     /locus_tag="ACG8KN_001139"
     CDS             complement(4255..5628)
                     /locus_tag="ACG8KN_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9188209.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(5654..5938)
                     /locus_tag="ACG8KN_001140"
     CDS             complement(5654..5938)
                     /locus_tag="ACG8KN_001140"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9188210.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(5940..7955)
                     /locus_tag="ACG8KN_001141"
     CDS             complement(5940..7955)
                     /locus_tag="ACG8KN_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9188211.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8134..8439)
                     /locus_tag="ACG8KN_001142"
     CDS             complement(8134..8439)
                     /locus_tag="ACG8KN_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFP9188212.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(8528..9559)
                     /locus_tag="ACG8KN_001143"
     CDS             complement(8528..9559)
                     /locus_tag="ACG8KN_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9188213.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            9766..10641
                     /locus_tag="ACG8KN_001144"
     CDS             9766..10641
                     /locus_tag="ACG8KN_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9188214.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(10793..11635)
                     /locus_tag="ACG8KN_001145"
     CDS             complement(10793..11635)
                     /locus_tag="ACG8KN_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9188215.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(11727..13604)
                     /locus_tag="ACG8KN_001146"
     CDS             complement(11727..13604)
                     /locus_tag="ACG8KN_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9188216.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(13601..15022)
                     /locus_tag="ACG8KN_001147"
     CDS             complement(13601..15022)
                     /locus_tag="ACG8KN_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9188217.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQAATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQTEIAKNGA
                     SSGTFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKAPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGISTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15025..18414)
                     /locus_tag="ACG8KN_001148"
     CDS             complement(15025..18414)
                     /locus_tag="ACG8KN_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9188218.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDCVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNSWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKNLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            18671..20062
                     /locus_tag="ACG8KN_001149"
     CDS             18671..20062
                     /locus_tag="ACG8KN_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9188219.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(20135..20782)
                     /locus_tag="ACG8KN_001150"
     CDS             complement(20135..20782)
                     /locus_tag="ACG8KN_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9188220.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(20790..21476)
                     /locus_tag="ACG8KN_001151"
     CDS             complement(20790..21476)
                     /locus_tag="ACG8KN_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188221.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(21482..22555)
                     /locus_tag="ACG8KN_001152"
     CDS             complement(21482..22555)
                     /locus_tag="ACG8KN_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188222.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            22777..23451
                     /locus_tag="ACG8KN_001153"
     CDS             22777..23451
                     /locus_tag="ACG8KN_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9188223.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQPPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            23456..24796
                     /locus_tag="ACG8KN_001154"
     CDS             23456..24796
                     /locus_tag="ACG8KN_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9188224.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMATIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(24945..25931)
                     /locus_tag="ACG8KN_001155"
     CDS             complement(24945..25931)
                     /locus_tag="ACG8KN_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFP9188225.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDENLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7527 a         4911 c         4773 g         8741 t
ORIGIN      
        1 tttcttttaa cgtacaagaa gtaaaatgct tatcgatata atctaatact ctaagcatct
       61 cattttctgc gcatccatct gctgatgaaa agctttgttg ttctaataaa tgagtcaatt
      121 cgatcagtaa tagtgatgta tagagattta acgaacggat cttatgactg tatgtatcaa
      181 aataaatcaa cagcatattt tcaatcaaat gcgtcaaacg acttgttttt ccgccgcgaa
      241 aatgaatgaa ttgtgtatgt tccccttttt cttttgatgc atttatcaaa aaagaagaaa
      301 gtagattatt tgtgcttgcc aaatgattca atatctctgt agataaggtt tcttctttta
      361 ataagatgtt gattaaaata tcctcttgcc ctgtgtattc gattctttga gtagcttctt
      421 tatcaatgat caccacgtca ctttcttcca tctctatcct ttctccatta ataatttgta
      481 aacatgtacc gctatacata taattgattt caacaaaatc atgtgtatgc tcgggaactt
      541 ccgcatatcg acggtgttta ctgatatgaa tatttccctg ttgaaataga tcccctcggt
      601 ggatttttgg cggtgtctga agatggtaag ctaagtttaa atcattgata ttcattcctt
      661 gttcacgctg cagagtttcg attgattctt cgttacgcaa aaaaaatgtc taattcagct
      721 agttccaatt gactccctcc tttttgtaag cggtttcttt cgcttattag ttcacgctgt
      781 actgctatta tatgcaaagc tatttttggt ttcataaaga ctttctatgg ttgggacatg
      841 aacttcctgt gattccgcta ttgagagaat aaccacttca tactatattt ttgataagcg
      901 cttacaaaaa tgaaaggaac atgcttatga ataaaaagat gatgaaattt tctctgctgt
      961 cagtctctct tattttgact tctgctggtg cagtcacgtc tgcaattccc agtatgtcag
     1021 ctacttatgc cacgcgttct ttatctgctg ttgaattatt aacgactgct ccctctttta
     1081 tggtcactat ttttgttttg attagtagtt ttattgctag gaaaatcgga caaaaacaaa
     1141 ctgtgatcat cggattagtg attgcatcga tttatggtat cctccccctc ttctcacaaa
     1201 gttacagtat tgtcttagct tcaagagctg gactaggtat tggctttgga ttgatcaatt
     1261 ctttggcagt cagcatgatt gctgcctttt atcacggaga tgaacgagcc tctatgattg
     1321 gttttcaaag tgcatttcaa ggatttggtg cagctttaat gacatttgtt gcaggacagc
     1381 tagtacagtt cggttggcaa tatacctttt tagtctacgc catcagctta cctattcttg
     1441 tcttgtttgt tttgtttgtt cctacacctg aaacaactga acttcaatca catggcacag
     1501 ccccaacaag acaagtaatc aaaacgaatc ctaaaatgat tagttatggt atttttctct
     1561 tgctggtcat cgtggtatat aatgctgtat cgattaaatt agccacagtc ctgacgactt
     1621 attctctagg aaacgaaact aatgcagcga atatcttaac aatcatgcaa ttggcaagta
     1681 tggctgccgg agtttgtttt ggctggcttc agacaaaagc acatcgctat atgcttacac
     1741 tttctttact actgatggga atcggctttg tattgattgc cactgtgata aacctgtatg
     1801 tgattgggtt tggtgcgatc ctaacaggaa tttctttttc gttattcatt ccttacttat
     1861 tcaaccaagt aagtatccaa gcgcccaagt acgcggagag tttcaatact tcaatcttat
     1921 tggttggcgc taatttaggt actttcattg caccatatgg tttgctcttt ctctcttttg
     1981 cttctcttgg gagtaaaaca cttgctgttt tcgtcaatgg cagtatcttg ctgttgatta
     2041 gcgcgcttat tagccttcta gtagtacaca ggacgaatag aaagacagtg ataagagtag
     2101 atgaagaaat aaaagaacaa ttgacaacaa aataaatttg atggaggtcg tgtaaaatga
     2161 caaatgaaaa acataaagat agatggcttt ggtatcctgg agactttgag atccgccatg
     2221 gactgctaca aaatttccaa cgggaagaac gaggttttga ctggccagcg tactggtaca
     2281 tggatgattg ccatcggaac gtcaagttta agagatatta ctttcttgat caaccttcca
     2341 tgtttaaagt gacgatccaa ggagttgggt atgtagaaat taatggacaa aaacatccat
     2401 gtggaaaatg gctgacttgt ccagctggta aagcaaaaat tcgtatcttt gtaggacata
     2461 catcagggct accagcgatg tttatcgagg gagacgaagt aaaatcagat attggctgga
     2521 cagcaagtaa ttttattgaa gaatatccag ctggctggag tccgctttat gtcgatatcg
     2581 caaaagatcc aaatcaaatc tattatcaaa aagaatgcgt ccatccagtc actgaaacgg
     2641 agattggcgg aggaatctta ttcgattttg ggcgagcagt gaatggctgt gtagaactaa
     2701 tatctctgga tgaccatatc gaatctatca ctctatgtta tggagagtcc gatgtagaag
     2761 ctatggatat cgattattgc tactataagc aaataggaat gacaagaaaa atgaaagctc
     2821 gtaaacgcgc ttttcgctat ttgtttatac cagaggtttc tactggaatg gttcaacttc
     2881 aagtttttca tgaattttta tctctttcga aacaagctgc tttttcaagc gattcttctt
     2941 tactgaatca gatctggtca gtatcagaag aaaccttcaa gttatgtagt gatttgtttt
     3001 ttatcgacgg tatcaaacga gatcgctgga tttggtctgg agatgcgtat caatcattca
     3061 tgatcaatca atatcttttt tttgacgagg aaatcaataa acgaacgata ttagcattaa
     3121 gaggacaaaa tgaaatcaag cagcatatga atactatcgt tgactattcg atcttgtggt
     3181 tgattggtat cgaaaaccac tatatgatga cgaaagacta tgaattttta cgcatcgtct
     3241 accctaagat gcaatcatta atgagatact tgatggagca aacgaatgaa ttagggttta
     3301 tttatgggag agcaaaagat tggatattta ttgattggtc tgaaatggat aaagaaggaa
     3361 cgcttgctgc agaacaagtc ttattactga aagcctatca ttccatgatc acttgcggag
     3421 atatcttagc tgaaacatat ggagagaacg aaaattatca tactcatctc tatcaagaaa
     3481 aatatcaact attgaaacaa aacctatttg actacttttg ggatgaagaa cttggcgctt
     3541 ttatcgattg ctatgaatct gggagaagaa atgtgacgcg tcatgcgaat attttcgctg
     3601 ttttattcga ttttgctaac aaagaccaac agcaatcgat cttaaagaat gtcttattga
     3661 atgacactat cacacagatt actactccat atttcaaatt ctatgaacaa gatgctttat
     3721 gcaaactcgg acagacgaat atcgtctacc aaacgattct tgattattgg ggagggatgt
     3781 tatctcatga tgcggtaact ttctgggaag aatacgaccc tactcaaaca ggaaatgaaa
     3841 agtacagtat gtatggagat ccttatggaa aaagcttatg ccatgcatgg ggagcaagcc
     3901 caatctatct tttaggacgc tattttatcg gtttgtatcc aacaaagcca ggttatgaag
     3961 aatttatgat acaacctaat ctagattcat ttgaaacact gaattgtacg ctacctatca
     4021 aagatggaaa gatttcgctt gaaaaaacaa aagataagat cgtgattact tctgatagaa
     4081 caggcggaac agtgaaaatg aatggtaaaa gttatcctct tctagcaaaa cagccacttg
     4141 tcttaacaag ctattcaaaa ataaatgcat aaaacaaaaa actgcttgcg acatatcaga
     4201 agactttaaa tcttcttctg atattgtcca agcaattttt attttgaagt ttgcttaatt
     4261 ctgttcttta gaaacaatgt ttgttctttt tccggcaaaa agaaagctag ataatacgac
     4321 aagtaaaaag aaagcgatga ttccaactgt caaggctatt tgaccgtatt ctcctagttt
     4381 cccaagtaca agtcctggta ctagataatc agtatcggca aatgtagaag aagcgatttt
     4441 aagatctcct aatactggaa ggataaataa tggcagccaa ctaatcaata agctattgaa
     4501 agcagaacct aataccgccc ctcttctacc gccagtcgag ttgccgaaga caccagaagc
     4561 agctccgcag aagaaatgtc ctactacccc aggaatgata actgtagttt gcaagcctat
     4621 catagcaaac atggatagtg ttcctacaaa gaagctaaca aaaaagccaa ttaatacagc
     4681 attaggtgca tatggaaaaa cgataggaac atctaatgct ggtttagaat cgggtacgag
     4741 ttttttagca atcccttgga atgctggaac gatttctgcc aacaccattc gaaccccttg
     4801 aaggacgata acaaacccag ctgtgaatgt tccagcttgg atcagtgcaa agattacagc
     4861 gttgtctcca ttgcttagtt ctctttcaac aaatccagga ccagctaaca aagcaagtgc
     4921 gacatatacg atcgacatca agagcatgat cgatacagta gaatctcgca aaaagctgaa
     4981 acttttagga atctttatgt cctctgtaga acggtctttg tttccaaaaa actttccgat
     5041 ccatccacta gaagcataac cgatatttcc ggtatgccct aacgcaactt gattgtttcc
     5101 agtgatgttc ttcataaacg gttgacaaag agcaggcgta atagttaata gtgttccttc
     5161 gaagaccccg cctaataaga tcatccacaa atttgcagta aaccccgcgc ttactaaaat
     5221 gactgctgtt agacaagaaa cataaagcat tgcttgacct gtaagaaaaa tgaatttata
     5281 tcttgtgaat ctagcaagta aaatattcac gatcatccct actagcatga tcaaagcagt
     5341 aggtgtaccg tattgttgca aagcaatcgc cactactgct tcattgctag gaactactcc
     5401 ttgagtatgc agagctgttt ggaacatcga agcaaaaggg gttagtgaat tactgattaa
     5461 accagcaccg ccgatcagta ctaggaatcc gacaaatgtt ttgatagttc cttggatcac
     5521 ttctgaaaat ttcttcttct gaagaacgag accaagcagt gcaatcaaag aaacgaggat
     5581 ggagggctga ctgacgattg acacaaacaa attcaacaat ttatccattg atagttcctc
     5641 ccttgaatca tttttattct tttgacaaga tcttcgttac tttttctttt acttcttcgc
     5701 tgtcaatgat cgattgcaaa ggtacgagct tttcatcagg tatagaagtt agcgtatctt
     5761 tcaaagaatg ctcaatgaag aaataatcag ccatatcgct agatacactt ccaacatccg
     5821 tatgacttac ttcaaactcg tccacatctg ctccaagatc tgcaagtgct tgtttgatgt
     5881 tcatctctac cataaaactt gttcctaatc cactttgaca tactgcaaaa attttcatat
     5941 cattcgtctc cttttcttat gatcgctaat atttcttctt ttgttgatgc gtgtaataaa
     6001 tcttgaagat tttgtttatt agaaagtagc tttgttaaat ttgccaaagc ccgaagatgt
     6061 gcctcattat cgatagcagc taaacagatg aacaaactaa tgggatgggc tggattatca
     6121 agcagatcca caggttgttg aagtttcagc aaagacatcc ctacttcgtt tacgccatct
     6181 tctggacgag catgaggtaa tgcgatgtga tcgccaatat ggataaacgc accatactgc
     6241 tccacgcgct cgatcatagc tttgatataa ctttcttcta ttttattttc tgataatagc
     6301 ggctgtgcgg caaatgtgat tgcttcttgc cagttttttg gttgatccga taattggatc
     6361 atttcttctg ttaataattc agatagcata ggacgttgat cctccttctt ttctaaaagt
     6421 ttgttcattt tacgatttaa tacttgatag agcttttctt cgtctacgcc ttcttttaat
     6481 tcgatataag gcagtagcgc cgacaacaat tcttgggcag aaggcattga aaatcctggt
     6541 atcagccaat ctctttgtac ttgattgatc agctgatttt tttccaagtc agtcatgatt
     6601 ggatgcagaa catagagaga ctttttctta tcagtaagtg tgagaggtac agtagaaaaa
     6661 ataatatcgt aagaagtttc gtcaacctcg tgtaagcggt cagctgaatt agtctctgta
     6721 aaaaagatca tcgggaataa ttgctgaagt tcacttttta agatcaatga agagctaatg
     6781 ccacttggac agacgatcac ggctcttatt tttctatttc tttcttctgc gtccgctttt
     6841 ctgatctctc cgccaaatag tatagtgaaa aaaccaatct cttcttcagg gatgctcttc
     6901 cctattcgtc tttctaacgg aagtaatgct tttcttgtca tttcgtagat ggaagcatat
     6961 tgatgcttaa tttgatcgat catcacatta ggtaaataaa aagaatagga aatacgaaaa
     7021 taggcaggga ccaagtgata aaagacattc atcaataatt cacgaaacga agcaaattct
     7081 acggccatca ggaattccat gcgatggatg acttctgaag aacattccag caaaaaatca
     7141 agtgcaggct cctggacatc tccttcaacg atcgatgcta agcaaagaga gaaaaatata
     7201 atctccgaat tttgatgttc ggaatagcca aggccagtca atacttgtcg actaatgtca
     7261 gtgactgcaa gagaagccaa cagttcagca tcttttacca gttccatgga tgtttttcca
     7321 attcgaatgg ttgtcaaact caaataataa gctaattcat ccattcgatt tggtgcgaat
     7381 cgaagatctg atagtgaaat cgttttatct agcaaatctc taactttggc gtaataatca
     7441 atggaaaggg ttgcggttaa atacgtgagc ccaaatcttc cagcaagtga ctgcatcaaa
     7501 cgagcagtgc gattttttgc ttctcttctt aatattgctt ctgaccctag cagcctaaaa
     7561 ccagttttcc tagtatacga cagctgacaa tcttccactt ctaattcatt tcttatcttt
     7621 ttgatatctg acaggactgt ccctttagaa acctgaagaa aatcttgaaa atgaaaaaca
     7681 gaaacagacg ttgcagtcga ataaaccatc aaataaagaa gatctcttcg ctcttgttcg
     7741 gtatacacag cttcaaatcg agtaatatcc aaaaattttt ctgcccagtc gattgctcgg
     7801 aatttcttta tacggatacg ggtatcatcc acatattcgg caaaaggagt taattgtttg
     7861 atcgcctcgc ttgtatttac ttcgatgatc tctgctgctt tttctagact gatttctctt
     7921 ggtgttttca ggagataact aattttgtag ttcacagcgc tgttcacctc ctacttatat
     7981 tctagtatgg tagcgctttt aaatcgaggg gtgtttggta caacttacag ttggattttt
     8041 tttacgactg taccgaacga tttctatcga atggatacag aaaaaaagca ggtgaccaaa
     8101 tgtctcctgc ttttatgaat tgatttcgtc cgatcaatga tgatcatgtt tattttttga
     8161 ggtcagttct tcaaattttt ctttgacatc ttcaaccgct ttttcgatat ctttttcaaa
     8221 gtgattgggc tcattaggct ttgtttcctc ttctacagaa tttgaaccag ataaagctgt
     8281 ttctttattt tctgtttcac cagagtttgt gccagctaat gattcttctt tgtcttctgt
     8341 ttcaccagaa ttagttcctg ctaaagccac ttcttcttct gttttagggg ccattgaacc
     8401 tcttaatcgt ttgtgttttt caccgtcata atttgtcatg ttggttcctc ctagtagttc
     8461 tatacttttt catcgtacaa taagacagtt tatgcgtcaa aaaacaaacc cgattcttac
     8521 aaatatgtca tatttttcct tctgattctt gaatgctttt gattccttct tccaaaattt
     8581 tcatttgtaa gattgtttct tcatctttga tgactttttc tgcgccatta actaatgtct
     8641 cgtaaatccc ttcgtagaca cggctgtaat caccaacctc ggaaattact ttttcttcat
     8701 gataactccc ttgttcatct atataggtca atacaccata atgttcgggc aaatcgatac
     8761 caaagtcttc atgatcaggc atgtaaaaca ttttcagatg ttcttcttgt cgatcttttg
     8821 tttgtttcac gaacatccct ttttttccgt aaacgacgaa acttggacgt tctttgatgc
     8881 ggaaataact ggattttatc gaaattttca tatttccata gaaaaaatcc agatcgaagt
     8941 aatcattcat tcgtcctttc cctaaaagtt gacgtacatc atagctgata tcatctggtt
     9001 cgccgaaata agaaagaact tgatctactg tatgacaagc atgtccatat aaatagctgt
     9061 tcgcagtaga aaattcatgc gtattttctg gtacttccgg tctgaaataa tcataatgca
     9121 tttctacttc taaaatatcc cctaattttc cgctttcgat tactttttgg actgtcaaga
     9181 agtcagaatc gaaccgtctg ttttggtaac attggataaa tagacctttt tcttttgcta
     9241 atgcaaataa ctcttctgct tcctttgacg tttctgcaaa aggtttctct actagtacat
     9301 tcttaccatg ttcgagtgcc atttttccat attcataatg aaattgacta ggtgtagtga
     9361 tgacaactaa ttggatctca ggatcatccc aaagatcaga cagctgatca gtatattcga
     9421 tgtcttcgaa tgttttccac actggttcag atggggaaaa aatttttttg atattgattt
     9481 tgttcgtaag ttttcttgaa aaaggcaaat gatagcgatt tgtactcttt ccattgccaa
     9541 gataagcgat tgtaagcatg agtggtgcct ccttttattg atgttttcag tatatcctag
     9601 aaaagcgcag aaagtattta taatagctat tctggtaaaa aagttcagaa aaacacttgt
     9661 tatcattcgg tttggtgaaa ttaatgttca aagtaaggat aaaaatcaaa aagatagtag
     9721 cggaaccaag tgtttatcag taagatagaa tttggaggtc agatcatgct tattgaagaa
     9781 aaactaacaa aacaagagtt gtttaccacg acagagaaac ggattgtcga ttacatccgt
     9841 aggaatatcg aagctgcagt ctacatgacg attgaagaat tagcaaaagc tacgtatact
     9901 tcccattcag ctatcattcg tttatgcaaa aaaatgggat tcagcggatt taaagaattc
     9961 cgtttcgagc tggggcggga agttcatcaa ttgattgctc agttcaatca aacgattgat
    10021 cctaattttc ctttttcaag tgaagatagc actcagcaaa ttgcaaagaa aatggcggat
    10081 ttatcgatcc agtcaataaa aaaagcacaa ttgcagattg aacatgaaga tttagatcaa
    10141 atcggtaaag tattgaacaa ggcacagcga atctttcttt ttgcaaaagg tgattctcaa
    10201 atcacggcaa ggaaattcca gaataaaatg gtcaagttga ataagttttt gatcatggct
    10261 gaagaataca gtgattcttc ttggaatgca gccaatttga ctagcgagga ttgcgccatt
    10321 tttattagct acagcggtcg catccatcat tatgaacgaa taatgaccta tttgaagcat
    10381 gtagaggccc ctaccttgct gattaccgga aatcagcatt cagaaatggc caaacaggct
    10441 gatatgtgtc ttgttatatc gcaggaggaa tatgattttg ctaaagtagc gactttctcc
    10501 tctcaaattg ccttcgatta cgtattaaat acgttattct cagtgatata ttctcaaaat
    10561 tttgaagaga atatccgcaa cttgaaaaac aaacaagaat tgatgcaaaa aggccttctt
    10621 aaagaatatc ggaaaactta agaaaagatc ctcatttagt aataagaaag atagtgtttg
    10681 ataggctctc tttgatatca tcataaaaac aggctgaagt tttctttttc aaacctcagc
    10741 ctgtttgttt acggaacgat tcgaatagat atattataga aatgaggaat agctaacttc
    10801 tgtttcttat tttgaacgat tgacgaaaaa gatatataag aaggaggaaa atcagtatac
    10861 atgcagctaa caatccatac aggaaaaatc gtttgttctc ggatactttc tgtacttctt
    10921 tttgaacttc ttttgcatga taaggaaccc ggtgccctct gacaagtaag cgatgactgt
    10981 tgaccatata aggtgtacaa gttaggagtg tcacgaaatc ctgccctttt tcgattctca
    11041 gatcttttgt atcagtcggt tctaccgtct tgatctgatc gacttgataa gcgagtgtcc
    11101 gtccattgat ttcgatatag aaagaatcac ctaaagtcag tttcggtaag tctgtgaaca
    11161 actttgcttg aggcagtcct cgatggctgg aaataacggc atgtgtattt tctccccccg
    11221 taggataaga cgttccttcc aataatgagg cacctttttc tagcagcaca gtattcgttt
    11281 catcaaaaat tggcaaatgg acattgatct cagggatcgt caaaataccg atcgtatgtt
    11341 tctcaaaata ggttctatcc tttttcgcag gttctttttt cttagtaaag ggatctgcac
    11401 ctggatttcc accttctttt gctaattgct ggtttttctt tgtcatgttt tcttggattt
    11461 tagccatgac ttcttcattt tctttgaccg cttgttgctg ataatgagag atgatctgct
    11521 gatccagata attattcagc gcgtcgctaa caaatggata agctagtatc cctattcctg
    11581 agagaagcaa gaaaagcatc agcagatcta gaagtttcct tttctttggt ttcattcgtg
    11641 ttctccttgt gatgactgtt tgataataaa agaaaaagga gcatttgcgt aatccgctgc
    11701 tcctttttcc aattcttgca aaaagactaa atctgactgt gcttgcgtct agcaaagtac
    11761 agtccagcaa tcagtagaag gactactcct gcaccgatat acacatagat tcccttaccg
    11821 cctgttgaag gaagtgtacc tttgtgttta tttggtattt tttcaggaga aatcaactgt
    11881 cctgctgtta cataagattg ttcatcgata gtaaatgcta cacggtttgt taatggaaca
    11941 tatttttctg gcgctttcgt ttcttccaga tagtacgtgc catattttag acctgtcaca
    12001 tcgattaaac cgttacttgt ggttgtaaaa accgttgctg attctttcgc cgatacccag
    12061 ctgacggctt ttgtggatgg gtcgatcgat aaatatttcg ctgtgtcact atcttgatca
    12121 cgaacgacga attctgctcc agcaagtgtt tgatctgatg taacgtcacc atctacttta
    12181 acaaatcgtt tgcccccagt aacgacatcg actgacggtg gtgtttgatc atttgtatgc
    12241 ccgttatcga catttgcttg gttagaaaat cctttggttg gatctgcttt ttcgttcaaa
    12301 tgcatatagt aaacaaattt cagcgtacca ccaggagtga gtgaaggaat ataattcgag
    12361 tcaactgaaa ccgtgaatcc gtttgtttgt tcagtgacag aataatttgt tggatcgatt
    12421 tctttatccc catcatacaa gacatacgca tacgttcctg aagtttcatg atcaaatgtc
    12481 aaagcggcgt catgagtatc aatgagtttg aatgtcgtgt acttgttttg tgttccttct
    12541 ttatcagcga tacctaaagg aatattgaca gagatctcat attggatttt ttctccgatt
    12601 gcgacatctt ttccattcaa ttgaggtgtt gttttatcaa ctttggacgt atcatttttg
    12661 attgtctttt ctacaggagt ttgactattt gccaagatct caaacggcgt gtttgtttga
    12721 ttatcaatca tttccgcatt atcaggagct tttacttctt ctaaattata ttttccaacc
    12781 tctaaatgat tgacgatcaa ctgacctttt tcaatcgttg cctcggcaaa gtcattgttg
    12841 ccgatgtcat aagaatgacc agtaatgaaa tgtttggctt tggtttgatc cgttgtccaa
    12901 gtgtacaatc catctgtgac actttggatg tattttttga cgcttggtct tccttcttct
    12961 ttagaaataa taaattctgc tccatttagt gcttcgtttt cggcagtacc gatttttgta
    13021 actttcaacg ttccatcatt accgactgta tttttagggt agagatggat agtatctagt
    13081 tcttctgtcc cgtatttata agagccatct gcttgtttga tcatctcata tacagggaaa
    13141 gctaaaacca tgtttgcggc agctgacact ccgtcttttg gttcttcttt gatcgtgtag
    13201 actgcatctt tcccattttg ttttttaggt aaagataaag tgacatttcc atcagcatct
    13261 gtcgttcctg aagcaactgg tgtaccagga gtcaaagact ggactgcttg ttttgctgca
    13321 tccacggacg ctcctttatc tcgttgcgca taaaattctt gagtgacgtt ataaactgaa
    13381 aatgaaatat cggctaatcc ttggtattga tcgaattcgc tcatttcttt cccgctgttt
    13441 tggattaaag gatcgggtaa atcagtcatt ttctttttat gaaggatgac ttgagctgct
    13501 gtctcctctg caaacacttt ttttgcgccg aagctgtttg tgagtaatgg taaaataagg
    13561 aaaaggactc ctaacataac gtttattttt ttatgatttt tcattttttc actcctgttc
    13621 tttttttgat aaacaaccag attgcaaagc ttgcgaatac cataccaaca acgcctaaaa
    13681 gtgtattttt cgtttcgttt gtttttggaa gaaatggacg gctattttct tttttattga
    13741 ctactttcaa tatactgtcc gcctctttat cctcagaaag aaaagttgaa atggtaaacg
    13801 gaatttctgt ttcacttaaa atataaccag aaggtgcttc gatctctttt aatcgataac
    13861 ttcctgtttt caacccggaa attgaaaaag cgccattttt atcggaaatc aggtgaagcg
    13921 catctgattc gttctttgtc cacttgtatc cgttggctgt ttgctggagg taatttcctg
    13981 ctttgttttt aataaggaat actgcgccag ctaatcgttt atctggttta tttgtctcga
    14041 ttttgacaaa ttgcttactc cctgtatata cagagacttt ctttgtactg ataccaaaac
    14101 caaaatccag tgttccgttg ttgttgattt ccttgttcgc ctgtgctgta ctgcttagct
    14161 gcatctgata agaaatggtc agtttttttc cagcatattt ttccaacgaa gggatcgaaa
    14221 ataaaacatc aaatccgtga ggttttttgt gtatgacaaa gccttctgtc agcttttctc
    14281 cattcgatga aatcgttaaa ctattaggta aaaacgttaa tgcttcatcc gcactatctg
    14341 acaattcgaa cttttgatag tcaaggatat ttacaggaat tgtcgtatgc aaggaataag
    14401 tgattttttc accaatcgta aaatcatttc taggctcttc taataccttc tcaaaaggtg
    14461 gatcagggta gtcgttttct tcatttttag gatacaaatg gatagttgaa agtttttgat
    14521 tgttttgtcc atgtacagga agaacaacta ccatattctc tgccttttct ttgacgactt
    14581 ctggtgcttt ggattcaatg aaaagatacg ctttatctct tttttcttga tctttagcgg
    14641 ccagagaaaa agaagcgata ccatcttcgt tgttaagagt tgtagttgtt gcttctgcgg
    14701 taaacgtacc ggaagacgca ccgttttttg cgatctctgt ttgtgcttct tctaccgttt
    14761 tgcccttttc ccgtagatgg taaaaagaat ctgtgacatc ataaacttgg aatgtgacac
    14821 catttaatcc tcgatacgtt tgtaataacg ctttttcttg tccgtcattt ggatgatttt
    14881 tcggcagttg cccgtttggg aaaagcaatt tgtgtaaagt aatctgaacc atttctgttt
    14941 ctgtttctgt tgctgcctga gtaaaagctg gtttaaatag taacaagaag agacacatac
    15001 taagccaacc aagttttttc atatttagct gccccctctt tcttttttca aaaaaagata
    15061 ccaaatagaa aacgcacaac caatcatccc tactagatag attcctaaac ggccaattcc
    15121 tcccgtttca ggtaatggta cttttgcctg attcgtgatg tctaaagaaa tctggttgtt
    15181 tttggctcct ggtgacagaa cagattcatg atcttgtcca tccacttgaa ttgacccatc
    15241 ttcgtgtata actatagtaa ctggctcttt tagaccttgg tatccttctg gtgtaaaagt
    15301 ttcggtcagc gtataagttc caggttttaa attttcgaat agaaaggtat ctgtttcttg
    15361 tccatctttt ggcgattcta agtccatttc tggtccctgc agtctgaatt ttgctccttt
    15421 taatgtctgt ccgttgtctt cttttttgtg taccgttaga tcaaacgctt ttagtcgatt
    15481 ttgatgagtg agcgtccata cattatttgt catctcggta ttttttcccg tgtaaatgat
    15541 ttttccatat ttatcgattt ggaattcata atttcccggc gaagtatcat atccggcagg
    15601 accagctgtt tcaacgactc ggtatttacc aggtgtgagt gaatcaaaac tggcaagacc
    15661 tttttcattc gttgtttggc tgtcaattgg ctgataagta ccatttgctt cttttttctg
    15721 tagggaaaat gctgctcctt taagatttat ctctttgtcc gtcccttgca tcgcatactt
    15781 acgtactgct acaggaactt caacaaaagg attttcaata gtcaactgga tcgtatcatc
    15841 ggaagtagtg actgtttttc catcaatggt taccgtacca tcagaagcga tcttgatttc
    15901 ccaagtagtc tttttgctta atccatgccc ttctggagct tttgtttctg tcagcgtata
    15961 ggtcatttct ttttgcaatt tgacattttc tggaagagaa taggttccgt cgccatgatc
    16021 tgttagtaaa gtatcaatag aatctcctgt taatttgaag acagcaccga tgagattctt
    16081 ttcacctgta gaggaatttt tcgttatttt caagtttaac ggtgtgaatt gtttagtatt
    16141 tttccatgtc attgcatcta tttgttgaga atcgtatcct ggtacaggta attctttgat
    16201 tgtttggtaa ctgaatgctt gaccttgatt attgtattga ggtagtgata atatctcttg
    16261 ataactttct ccgctatttg cagataattt gtcaatgtct gcacgttccc acgaatttgt
    16321 agtatcttta gctggtttaa tgattcgaat atatccgttt ttccaagctg cagcatttgt
    16381 cgtatgttcc cgttgaatct caaaagtaac ttgatctgga cgatcagcta gattgttgtc
    16441 aaattcttcc cataactttt tgatgtgaag actgactccg ggagctttag cagatggtat
    16501 accgaattca gctaattcat tggtatcaat gtttggctgg aaagttgtcc tgccgttcat
    16561 ttgataccag aaatttggat ggaagtcctc attttctgtt tggattctca cttggtaatg
    16621 gatttggatt tcttggtctt ttcctaaata gatctgattg ctcttgatgg tatttccttc
    16681 tatggaaata gatggagtga ccgttgtagg acttgtcccc acactcttga ctgaaagtgt
    16741 accaggctga taaatgaatg gttctgcgat tggatcattt atttgtccat tgcttacagt
    16801 tgctgagatc tgtacagctt ttttggctag gtattcagag atatctgttg catgatcagc
    16861 tgattcatag taaagatcgc ctttttcatc tgatgaaacc atttgtcgca tacgagactc
    16921 tacttctgct tttgagagac cagctgccgg atcgctttga agttggatgc caagaccatg
    16981 tatttcgatt cctcgttctt tgagtgccat cgcttctccg atggttgcga taaacgtact
    17041 gtcgattctt ctggatagat tgttttggtc aggtgcatca tagattcttg agattagaga
    17101 agtatttccc ggccgatctt gcgtattaga aaactgagtt ccgtaataat tgctgcttga
    17161 ttgtgcgtgt acccgctgta ctttatagga aaatgttggt acacaatccg tcagcaaaac
    17221 gatcactttt ttatgtccat ttggaacgga tagcatgctt cctgcatctc ttagtgcttt
    17281 ttgtgtaaaa gtaccaccat ttgtccgtga aggtgtaatg gattttactt gatttttcac
    17341 tgaatcaaat gaacccatct gtactgcacc gttactgtag ctatatcctt cgcttgagta
    17401 gccgacatat cccatattga ttttgtctgt gataccgcta tctgctaaag tatcgacaaa
    17461 acgatcgaca ccaatcttta cttcaccgat ccgattattg tcgttcatac ttcctgacca
    17521 gtctacgacc aatacgagat caagaggcgt gatatctttt tgtacatttc cacgagcatt
    17581 caaataaaca tcaaacaatc cgggtgtact tgtttccttt gcatactttc gtatcgcata
    17641 gtctgcttga ttaccggttc ctccatattc gatatacgaa tgggtccgat cattaggatc
    17701 tccattccaa gaatttatac catcccattg atttgttccg tttttattgc cttgatgatc
    17761 aagaacattt gtatttcctg taggttgcca actggcttta ggataagtgc cctgatcatc
    17821 tgttgtatat tctgtaggaa tcgtttgatt agatggcaca ctcacagctg gcaaggataa
    17881 tgtaggcaat gtaaatgggc tgacagaatt ttcgttttct tttgatgatt cttcttctat
    17941 ttcgtctggc tttggtactg acacatctgt atctttctta ttttctgtct catcaataat
    18001 gtcatattct ttattatcga cgatcagctt gtttgcctgg ctgtggtaaa gtgctggatc
    18061 gattggaaaa ctcagatcga tagtttgttt agtgtttgat gagatttcca acgtaatttt
    18121 cccgctattt acctgataag catagccatc tccttctttt ctttccacat cacttaagta
    18181 attttcgact agaccaattt ctttttctat cttttcagca ctggtgttat tcgcatttag
    18241 cttgatgttc agtgcttttt ttccagactg atccgaaatg acttcagctg aaattgtttc
    18301 taaaactgtt ggatcatctg tagtgtctgc tattgcagat aatggcacaa agtttgataa
    18361 actcaatatt atcagcatga aaataacttt cagtttcttc cctgtggtcg tcatttttcc
    18421 cctccttttt ctttttttct gttttgacgt gaatagtgaa aattattaaa ataaacaggc
    18481 gttacataat tttatttatt ttaatatgat aattattata cgaagggcgc taaagggaat
    18541 cacaccaatt ttcataccaa ttggtttcat ttatgaagtt gattccttcg attaatgagc
    18601 gtttcattga aagaaaaggg ttgagggaga aaaacatatg atcaggttgt tggaatgaaa
    18661 ctatagaaag atggaaaaaa tcattcgaat tctgtccatt tctgacaagt ggatgactaa
    18721 aaaagaactt gccaaacgaa ttggtagttc agaatctaca tttgtaaggt atatcgaaga
    18781 gatcaaaatc cgctggggaa cggatgtaac gattcaatct tcacaaaaat tggggtatcg
    18841 tcttgaacat ttgaatgtac cagcttattt aaatgttctg atggaaatgt cgcaaagttc
    18901 tacaaacagt cgtttattaa atgagattat tgtcaatccg ggtaaaacga ttgagtatta
    18961 ttgtgaaaaa atttccatca gccggtcttc ttttacacgt aagttgaaac aatgcaatca
    19021 aattttagaa agctatttat taaaagtgat cgttgaccaa ggttttcagt taactagcct
    19081 taaaagtgag ctccagttgc gtatctttgt cacctttttc tttttaagtt actacggaag
    19141 acgcacgttg ccatttccaa tcgaaaaaaa gaagatcaaa gaaattatgg tcaagaataa
    19201 ttgtcagatg aatttgtttc aggaagaaag cccatatgag caatctttct ttattatgta
    19261 ttttgttgtg catctcatga gagaatccca aggaactcca tttgaatgga aagctggaga
    19321 ctcttatgta cagttgataa atatcgataa aaaagacttc ttaatcttga aaaatctctt
    19381 aggaaaattg aagtacagca gctatataaa agtagtgaac tattttgtca atacgatttt
    19441 ttcagaattt tcggagaaag aaaaaaatga aatcagtgag cagatacgaa aaaatatgtc
    19501 ctactgttcg ttttcatccg ctgattgcaa agaaaaaaac gaagaatatg ttgttcaatt
    19561 attaacgcaa atttattttt tctcccgtat tttcccatac gatacgactc atttgacgat
    19621 ccgtgcaact tactttgcac agcagatgca acaccgccag atacgtctat atgctggatt
    19681 tcgtgcagaa cttcaatttt attccactct actgaatcag aatctgattt attatttatc
    19741 tagctatatc ttttggatgc ttatcggtaa tcctgaaatt caccattata cttctgataa
    19801 aaaaatactg attatcagcg acttaagtct tcgacatagt caatatattg aagaatatat
    19861 cagtgatatt cttgctgtgc ataaaattca ctctgagaca actgctatta ctgaagatca
    19921 gctgtcgaaa tacaatcttc tcgagtatga tctgatcgtt acgaatcaac ccattttgaa
    19981 tgcccatgtt cctcatatat tgattgatga tagtgtgtca ttcgcaaatg aagaagaact
    20041 catccgctta ttcgagttat gaataatcaa aaaacagcta tgtataaacc accctgataa
    20101 aaatttcagg acaaatacac atagctgttt ctttttattg tattccttca gcaatccaac
    20161 gagatttata gcgaaagtct tttaacaact cttcttttgt ccaattattt gcaatgagat
    20221 ccgtcaaagt gttcattacg agggtcatgg aaaagtatag gaaactaact gcttcttccg
    20281 acgattgaat ggagaaatga ttgattctta ataattccag ccatttttcg ttcattggag
    20341 aatcagccga tagttctcta cgtttggttg tctgctgatc atttcgctgg gttaacaagt
    20401 ataacctttg ccaatagata ctttcgtgat ctaagtcgca gcaccactct aaatattttt
    20461 cgatccccaa aaagaaatgt tgtttgtttt cgctaatgaa ttttaagata tcttgatgga
    20521 ctatgtgaga actgcttttg atcgtataca aataagcatc ttccaaatct tgaaaatatt
    20581 tataaaaagc accgcgcgac atccccatct tgtcaacgat ttctgagact ttgacttggc
    20641 tgatgtgctg tgagcaaaat atctccagta ataggtcatc gattctctgt ttctttgcgt
    20701 tcgataaatt gaaaaaagtt tcttttggca tccacatctc tcctttatct ctgattatac
    20761 gtggatagcc cttcattgtc atgaaggatt cattccttct ttttctcttc ttctgtcaag
    20821 tacccatctt ccattcgata aacgcgatca ctccattgaa tcatacggac atcatgagta
    20881 accataatag tcgctttttg tttctcatga gcttctttaa ctagtaattt gacgacttca
    20941 tatgcatgat ctgtatccaa actagctgtt ggttcgtctg ctaagatcaa gcttggctca
    21001 ttatataaag cgcgagcaat tgccactctc tgtctttctc caccagaaag gtctttagga
    21061 taactatttt taagtgactg gatatctaaa gattgcagca aggaatcgat ttttgttgaa
    21121 tcttcttttt ttgaagtaac ttggtcaact agttcaaatt gttcctttac cgttaaaaaa
    21181 ggaatcaaat tagagctttg gagaataaat cctatttctt taaagcgtaa atctgctcgt
    21241 tttttttctg gaagctcagt tagtgagtga ccgttgatca ctatttctcc ggaagtcggt
    21301 gtttgcaatc ctcctgaaat ggtaaggaaa gtacttttac ctgaaccaga aggaccaatg
    21361 atgctgacga attctccttc ctttacataa aaatcaattg ctttcaaagc tgagaccttt
    21421 gtatgaccag aaccaaatgt tttggtaact tttttcatcg ttaatatttt tttcattttt
    21481 attaacctcc tattgctgta attgggtcta ctttggttat cgtacgtatc gaaaacaatc
    21541 cgcctagcat tgcgacaatg atcaaaatcc cactatatag caaccattgt gaccaaaaaa
    21601 cagcaaaagg catcgcgctt ggtaaaatca gactagtcaa ataagcaaac aaaagagcaa
    21661 gcagaacgcc taccacccca acaataaagg actgtgctac taaagattta gcgatgaaga
    21721 aatttttgat cccttgtgct ttcattactc caaaaatagc agtcttctga agtgtcatca
    21781 cgtacatgaa aataccgacc acggcagcaa ccactaagaa caaaaagtat atcatcgcat
    21841 ttaaggtcat attttgggca gaatagccag gaatactttc aatgaatgtc ccaatagtta
    21901 ataactgtag acccttcgat gctttttctt tcgaaatttt agctgaatcc tttattacga
    21961 tcccattgat cggctgttct tcttcagaag caaacggctg ttgtccatac ttcaaagcag
    22021 tccacgtatc tagggacgca tagatgacgg gagaaactgt gtaataagtt tcaggaaaga
    22081 taccgacaat cgtcaaagga tcgtcgtaac tgccaatttt gatcgtatct ccaattttat
    22141 agcctgaatc agccagattt tgagagatga tgatttcatt ctttgtttta aattcatgcc
    22201 ctttcgtgag ttttggtaat aaaaatgctt ctttcgttgt gccgaaaact gtaacatttt
    22261 ctttgttttt tcctttgatt gctccgctgt acaatccgat tggtgctttc tctcctcctt
    22321 caatgtattt cagatctcct cttgttaatt gtgaagctgc aaatacttga tttgcatcct
    22381 ccgacaaaac aatctcttgt gcgtcccaat catcgattgc ttttttaaac tcttctgaaa
    22441 gtccattcgc tagtcctgac aacatgaata cgacatacgc aatcaagaac ataataccga
    22501 cgatcaagcc atatcgtaat ttagaatagc gaatttcttt gatagctaga aacatttgtt
    22561 tttcctccct atgatagatt gatagtgaca aaatgtcact tgttgaatag tgtaactcta
    22621 aagtgacaat ttgtcacttg tgttgttttt ttcttatcaa taaattgtct aagacatctt
    22681 tcgataaaac cttaacatga gtcataaaaa atttatttaa gccttattta tgctttccat
    22741 gataaactaa agtgaccaaa tcaatgaggt gggaaaatga ttaaactatt agtagtagaa
    22801 gacgataaag tattatccaa taatataaaa gaaatactcg ctgcaatcgg cgagataaca
    22861 caagtttatg atggtagtga aggactgttc gagttggaaa gtggagttta tgatcttgca
    22921 atcttggatt tgatgatgcc ggaaatgaat ggatatgaag tgttagcaaa aatacggaaa
    22981 gaaaaaatac agcctccagt attgattttg actgcaaaag atagtttgga agataagatt
    23041 gaaggatttg aacgaggtgc agatgattat ctaacaaaac ctttctatcg agaagaactg
    23101 atcatgcgcg tgaaagcatt attgaaacgc tcgctaggtc tctttgatga aaatgttctg
    23161 aagtataagg atatcagttg tgatttaaaa agcaatgaag tcttgtataa agaacaagtt
    23221 ttgcccatac aaggaaaaga gtttgaattg ctcgtttatt ttttacaaaa taagggaatc
    23281 attttgacaa aagagcagat atttgaccgg atctggggat ttgattctga tacgacgatt
    23341 acagttattg aagtttatat gagcaactta cgaaaacatc tgcgaagtac tggattggat
    23401 aaagaaatca aaaccttacg taatgtcggt tatatactgc aaagggtgtg atgcaatgaa
    23461 tggagatatt acacgaaagc aacgtagtcg cttttttgcg acaaatgtag cagcatttgc
    23521 ttttatcttt tttcttttgg gattcattac tttacagata ttaaaccaat ctgcatacag
    23581 agaaacagat ctgtcattag agaaaataaa ttttgattct cgaacagtac aattagagat
    23641 acaaagatat cagcaaggcg atcccttttt aaacccgact caaacggaac aaaacagaac
    23701 aaacatggaa cagccattaa tggaaccatc aaatgaccgt tttaacactc aagtgatctt
    23761 gtggtctaaa aatggtgaga tattgaataa agcagctatt ggtgggcgtt tcaaccaaat
    23821 caaagaatta aaaatggata cgaaaaatct gaataccatc caaaatattg aattaaatga
    23881 agacagtggc tcatctgccc tctctttccg ttcgattacg aaaaaagcgg agacgaatca
    23941 agatggcgtt gcctacatcc aaattttaga aaatacaaat caaatcaaga attcattagc
    24001 gacttttcgc acgattatga ttttgtgtat gattgttttt tggattctct ctattggtat
    24061 cagttattac ttgtctagtc taagcatgcg ccctattatt ctttcttgga gaagacaaaa
    24121 agaatttgtt gagaatgctt ctcatgaatt gcgcacaccg ttaacgatta ttcaaaatag
    24181 tttggaacat ctttttacca agcctaatca tactattatt gaagaatccg aaagcatcgc
    24241 tcaagcattg tcagagacga ggcgcctcac tggattgact tcggatttgc tgacaatcgc
    24301 tcggaatgat tctgatcagc agctgctttc aaagcaaatg atcaatacac aagaatatat
    24361 cgaacaactt gttaaaccat ttcaagagat ggccaccata gatggaaaag agtttattct
    24421 ccataacgat gcaacattag aagtaccaat tgatgaaaaa aaaatccatc aagttttggt
    24481 tattttgctg gataatgcat taaaatatac gaatccagaa gataagatca ttttggattc
    24541 atctgttaac cataaagaat ggctgattca cgtaaagaat actggccctt ctatcagtga
    24601 agaagatcaa gcaaggattt ttgatcgatt ttatcgggaa gatcaatctc gggcgaaaga
    24661 gactggcgga tacgggcttg gcttggcaat tgcaaaacag gtaatcgaac agcatcatgg
    24721 acagatttct gtatccaatt atcttccaca aggagtcgat tttaaagtcc aattgccgtt
    24781 gaagcctaaa aaataatgct tatccctaaa aaagggctgt gacaaaaatc ttgtcacagc
    24841 acttttttgt tgcgaataaa cggtgttcca gaagtagctt cttcggaaat aagccgaaat
    24901 ttctttaagc tgaccacttc tgtcacaacc tctttagctt acgcttacga ttttttattg
    24961 cggtcttcaa tgtgtacatc accgcttgtg tcaatatcca attcttctcg gctaacatct
    25021 tctgtcactt tttcaacatc ttcatgttct tctttatgaa tcccaacttc ttcacgaata
    25081 actgtatgtt tgcttacatc tacttgctct tctttgattg ggatagtaat cgtgtcatct
    25141 tccaagtttt cgtctgttcc ctgtgaagat tgatctgtta ctggtttgcg ttcgatgacg
    25201 atttcttcac gtttgaccgg tacttctacc gtttcagtat cgttgaccac atgtttgtgg
    25261 atgtcgactt ctccagttgt cacatcatgt gtatttacat ctaatttttc ttcttttagt
    25321 tgaattgtat catcatctag atttggtttg cgatcgatgt cttcagtctt gcttgtaccc
    25381 attgtatcag tctcgccagg tacgtccatc ggatcagttc ctggtgtcat attcaatgga
    25441 tccgttgtat tttgaaagcc tgtcgtgcca cctacgtcca ttggatctgt tccagtgcca
    25501 gtttctaatc cagtccccat tgtgcttgct gggctaacag tatcttgtgt atccattgta
    25561 tcttctgttg tttctggctg acgatagcct tttactgtga tgacaaattt tccatcagaa
    25621 atatctcctc tatattgata taaaggatca ttttctggtg tagccgtctc agaatcatag
    25681 gtatcaaaag aaaaagtatc ttttattttt tcccataaag aacggtcttc ttcaccatcc
    25741 atccgatttt catctgtttc aacatctata ccagacaatc cgtcaaatcc tagtctagat
    25801 gctgcttcat cagtagtata aataacgata tcatctcgtt catacccttc gttacgcaac
    25861 cgttcaatta ctgataatgc ttcttccctc gtactatagc ttccgatgac cgttaaattt
    25921 ttctcttcca tgtaaattcc tcctagcaaa ta
//
LOCUS       DAZKCZ010000030        24395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000030 DAZKCZ010000000
VERSION     DAZKCZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.030"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..748)
                     /locus_tag="ACG8KN_001156"
     CDS             complement(14..748)
                     /locus_tag="ACG8KN_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9188226.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1075..1485
                     /locus_tag="ACG8KN_001157"
     CDS             1075..1485
                     /locus_tag="ACG8KN_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9188227.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1478..2179
                     /locus_tag="ACG8KN_001158"
     CDS             1478..2179
                     /locus_tag="ACG8KN_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFP9188228.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2179..3765
                     /locus_tag="ACG8KN_001159"
     CDS             2179..3765
                     /locus_tag="ACG8KN_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9188229.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3784..4005
                     /locus_tag="ACG8KN_001160"
     CDS             3784..4005
                     /locus_tag="ACG8KN_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFP9188230.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4002..4763
                     /locus_tag="ACG8KN_001161"
     CDS             4002..4763
                     /locus_tag="ACG8KN_001161"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFP9188231.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5143..5652
                     /locus_tag="ACG8KN_001162"
     CDS             5143..5652
                     /locus_tag="ACG8KN_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9188232.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5727..6266)
                     /locus_tag="ACG8KN_001163"
     CDS             complement(5727..6266)
                     /locus_tag="ACG8KN_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFP9188233.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6406..7017)
                     /gene="rpsD"
                     /locus_tag="ACG8KN_001164"
     CDS             complement(6406..7017)
                     /gene="rpsD"
                     /locus_tag="ACG8KN_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9188234.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7279..8130
                     /locus_tag="ACG8KN_001165"
     CDS             7279..8130
                     /locus_tag="ACG8KN_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9188235.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8158..8793
                     /locus_tag="ACG8KN_001166"
     CDS             8158..8793
                     /locus_tag="ACG8KN_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFP9188236.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8813..9586
                     /locus_tag="ACG8KN_001167"
     CDS             8813..9586
                     /locus_tag="ACG8KN_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9188237.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            9994..10791
                     /locus_tag="ACG8KN_001168"
     CDS             9994..10791
                     /locus_tag="ACG8KN_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFP9188238.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10769..11182
                     /locus_tag="ACG8KN_001169"
     CDS             10769..11182
                     /locus_tag="ACG8KN_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9188239.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11166..13811
                     /gene="mutS"
                     /locus_tag="ACG8KN_001170"
     CDS             11166..13811
                     /gene="mutS"
                     /locus_tag="ACG8KN_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9188240.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13829..15937
                     /gene="mutL"
                     /locus_tag="ACG8KN_001171"
     CDS             13829..15937
                     /gene="mutL"
                     /locus_tag="ACG8KN_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9188241.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15959..16519
                     /locus_tag="ACG8KN_001172"
     CDS             15959..16519
                     /locus_tag="ACG8KN_001172"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFP9188242.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16604..18592)
                     /gene="treP"
                     /locus_tag="ACG8KN_001173"
     CDS             complement(16604..18592)
                     /gene="treP"
                     /locus_tag="ACG8KN_001173"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFP9188243.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18815..19528
                     /gene="treR"
                     /locus_tag="ACG8KN_001174"
     CDS             18815..19528
                     /gene="treR"
                     /locus_tag="ACG8KN_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFP9188244.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19605..20051
                     /gene="msrB"
                     /locus_tag="ACG8KN_001175"
     CDS             19605..20051
                     /gene="msrB"
                     /locus_tag="ACG8KN_001175"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9188245.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20221..20823
                     /gene="ruvA"
                     /locus_tag="ACG8KN_001176"
     CDS             20221..20823
                     /gene="ruvA"
                     /locus_tag="ACG8KN_001176"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9188246.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20836..21837
                     /gene="ruvB"
                     /locus_tag="ACG8KN_001177"
     CDS             20836..21837
                     /gene="ruvB"
                     /locus_tag="ACG8KN_001177"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9188247.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21866..22444
                     /locus_tag="ACG8KN_001178"
     CDS             21866..22444
                     /locus_tag="ACG8KN_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9188248.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22496..22978
                     /locus_tag="ACG8KN_001179"
     CDS             22496..22978
                     /locus_tag="ACG8KN_001179"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFP9188249.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23095..23469
                     /locus_tag="ACG8KN_001180"
     CDS             23095..23469
                     /locus_tag="ACG8KN_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188250.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23899..>24395
                     /locus_tag="ACG8KN_001181"
     CDS             23899..>24395
                     /locus_tag="ACG8KN_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFP9188251.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT         7997 a         4384 c         5208 g         6806 t
ORIGIN      
        1 atttagcttt tgattaagcc ataaataata atttatataa gaatactgct acgacaccag
       61 cacagatagg tgctacaact ggtacccatg catatcccca ttgagaagaa cctttatgtt
      121 tcaatggaag aagtgcgtgt acaagacgtg gagcaaagtc gcgggctggg tttagtccag
      181 gacctgtagg accaccaagt gaagcaacta atgtccatac aaggaaacca agtgctaagt
      241 gagctgtacc aagattatct tggaagaatg gtgcttttgt aatacctagg gcaccaaaga
      301 ataaaacgaa tgaaccaaag aattcattga cgaaaccatt gaatttagaa ccaacgctgt
      361 tgattgttga gaatgtacct aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt
      421 tgtaacaagc tactacgatc aattgtccaa tcgctgcacc aatcaattgt gcaatgatat
      481 aaggaacaac ttcagcccaa gggaatagac cattagatgc taaagcaatt gtaaatgctg
      541 ggttgatgtg gttaccgctg attccaccaa aaatcattgc tggcatcata accccgaatc
      601 catacccgac agcaatcagc atccagtcac tgccatatcc ttttgtacct tttaaatcta
      661 cgtttgctac agcaccgtta cctagaacga ttagtaatgc tgtaccaatc atttccgccg
      721 caagacgggt cattagtgag taatccataa atacctccaa aagttataaa aatgcaaaaa
      781 aaaaagaaac atcaacacga aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc
      841 agtcttaaca aatgtgaata aaaaaatgaa ttccattact tataagaaac cagaccaaca
      901 gtaaaactcc aactcttttt aaaagttctc cacaaatcac caagcttaac tataaatata
      961 ccaaaaaaaa aacgtttttt ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac
     1021 tttcagaaaa ggtttgttag taaaatgaaa gctatacgta aggagtgttg tttgatgaaa
     1081 ttttttcaag agtttttcat tattattttc ttttctgtat taggagaagg catacggatt
     1141 tttgctcatt tacctattcc tggaagtatc ttaggtattc tttttttatt tttagcattt
     1201 gaaatgcatc ttgttgatcc tgaaaaaatc ggagcaactg gtgatttttt attgaataat
     1261 ctagccattt tatttgttcc tgctggtgta ggtttattgg aatattttga tgatattgct
     1321 tcgatatggc ctgttttact gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca
     1381 gcgggaaaaa cggcagaagg tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca
     1441 gaaagggtat ctgtaaacga aatggaggag aatcaaattg actgagtttt ttgtgaatcc
     1501 attatttggc ttaagtttga ctttaggagt gtacgtatta tctgacaaat ttttgcggaa
     1561 atataaactg cctgttttga atcctctcgt attatcgatt gttgttatta tcttattttt
     1621 gcatttttct gggatcagct ataaagacta taatatcggg ggcaattttt tgactatgct
     1681 gattactcct gcgacagtgg cacttgccat ccctctttat aaaaacttcc gtttactgaa
     1741 agagaatttt tatcctgtaa tggctgcgat tttagcagga atagtcgcaa attgcttggt
     1801 tagtgtaggg ttcggttact tcttctctct tcataaagag atggtgatct cattactacc
     1861 taaatctgta acgacggcta tttctgtcga tttatctcat acgatgggcg gggtgaatgc
     1921 cgtgacactt gctattgttg tctctacagg tatttttggt tcgctgatcg ccactcatat
     1981 tttccgccta ttcaagatcg aaagtccagt tgctcgcggt gtggcgttag gaagtacttc
     2041 tcatgctatt ggtacagcaa aagcaatcga gattggtgaa atagaaggaa tcatttccgg
     2101 acttgcgata tgcgtcaacg gaatattgac cgttttattg ctaccgttat tctttcagcc
     2161 ttttgctggc ttgttttgat gcgaaaacca ctattttatg ctacaataac caaaacgttc
     2221 gagcataaaa aaggagggaa agacgtgtat ttgtcttccg agtatcttaa acaatttgat
     2281 aaagatctat attataagat tctcctccta gaaagttttg aggatcaagc ttggcatacc
     2341 gctgcacaat tagcgcaagt tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac
     2401 gagctgtcta aaaactatca gcagtttagc ggtaagacac accctttgtt tacaaaaaat
     2461 caccgttctg gttataactt ttatgatacg ctcgattcaa ttgaacatga gcgttttttg
     2521 atctatctcg tgcaaagtac gctgaaattc cagttgctgc acgacatttt ttttgaagaa
     2581 ttccatacaa tgtaccaatt tgcgcaaaag cattacatta gtgaatctac tgctcatcga
     2641 aaaatcaatg aatggaaaca gcaactgcaa acttacggaa ttcggctgca acgaggtaca
     2701 tatatcgctc aaggcgaaga agaaatcatt cgactttact tgcatatgac attctggcag
     2761 ttgtttcgag ggaaaatctg gccatttgaa acgatttctc aaatggacgt aaagaatatg
     2821 gcagaacata tcatggcttt ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg
     2881 gaatatatgc taggtgcctt ttttttaaga aaatcacaga agcattatgt tgtattgaat
     2941 gagaaaaaaa gacggctgat ttctgacaat ctgttgttcc agcgattttg ccaggtcatg
     3001 gagccggtat ttcctaatta ttttcaagta gaagacgagt taggcgcatt atttcttgta
     3061 ctgatgactc gtgaagaata ttatagtgat cctaagatac ggaagaaaat ttttgatttt
     3121 catcaacaag caaaaacacc gccctttaca gccctttcag aagcaaaagc cgctctttct
     3181 ctttatcagg aagagcaggg gctgccagca gaaaatctaa ccttcgaggc agaaaactat
     3241 ttattcagca gtcatttttt cgcctatctt tttcctaatg cgaaagaaac gattgatggc
     3301 aatagtagtg attttattaa tcatttagtt attgaaaaca aggaattgaa acaatggctc
     3361 gtacattttt ttgaaagtag acacaaacat cccaatcatc tggcatttaa aaatcatgcg
     3421 tttttgatgg ggcggtatct gactgtgttt aaaacattag gagcctttac tccacagctt
     3481 ccaaagatca cgatcctttt aatgacagat ttcccattat ttgaagagca actgttagag
     3541 gagggattac ggacattttt ccgtaatgag tatcagctga tttttctgcc aacagattat
     3601 cgggggagag aagttgatct tctgatttcg acaagtaaag tccaccgcaa accatgggcg
     3661 gatcttgact attttattgt gactgaagaa ctaaaattaa ttgattacat ccaactatct
     3721 caaaaatttg aaatgattca aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa
     3781 aagatgacat ttgaagaaat tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa
     3841 ggatggagtg gatttgaact ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt
     3901 ccggtgacgc cgtacttatt gatcaaaaca agtgacgaag gtttttccag cttcgctcct
     3961 acagtttgtg atattttagc agatgattgg gcgatcgtag aatgaaaaac cagtatccag
     4021 atctagctaa acaggttgta tttatcacag gagctgcttc tggaattggt gctgcgcaag
     4081 cccgagcgtt tcttgaacaa gatgcttttg tatttggatt agatcaaaag ttcggtaaaa
     4141 tggaagaaat ttctttgcga tttcctgaaa cttttgccta cgcactaggt gatgttcgac
     4201 aaatgaaaga actccaacaa gcggtatcga aatgccaaaa tcattttggt gaagttacga
     4261 ttttactgaa tacagcgggg atattggatg cctacaaacc cttgatggaa actgacgaat
     4321 cgctgtggga tctgatttat gagacgaatg tgaaaagcat gtatcagctg acaaaaatca
     4381 tccttcctga tatgatccgc caaaaaagcg gaacgatcat caatatggct tcgattgctg
     4441 gcttgattgc tggtggaggt gggatcgctt atacaagcgc gaaacatgcg attgtcggat
     4501 ttaccaaaca actcgctctg gattacgcag gacagggtat ccgagtaaaa ggaattgctc
     4561 caggagccat ccaaacacca atgaatgcgg ctgattttga agggaaaggt gagatggcag
     4621 agtgggtggc aaatgaaaca ccagtcaagc gatgggcaca gccggaagaa gtagctgagc
     4681 tgacgttatt tcttgcctct cctcaagcaa gctacataca aggaacgatc atcccaatcg
     4741 acggcggatg gttgttaaaa taaccttgtg tttttttcaa gcttctgtat gataaaacta
     4801 aagagagaaa caacggttga tgataaaaaa agtcgatgac tgttcccgac attcgatgcc
     4861 gaacagccag ttcaaaaaat tctttttaat aatcgttaat attcgaaact aagcggcttt
     4921 tcacgacctc ttgatataaa cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc
     4981 aaatgaaaaa tataaggagc tatttttatt tgaccattct tattacttgt tgcagggcag
     5041 ctttttcact accttctgcg acacttcagt tggaattttc ttcccatcct ttggtcgcgg
     5101 tcattaccaa gataaaatta tcttgcaaag gagaaataga acatggaaaa aaataatacc
     5161 cgttcagcca aacgctggac aacgacagat gtgactcgga tggcgctagt cactggttta
     5221 tatgtggcag tgacgctggt tttgtctgtc tttagctttg gagtgattca gattcgtctc
     5281 tcggagatgt tcaactatct gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg
     5341 ggagtagctt tggctaatat tgcttctcct cttggactga tagatgttgc agtaggaagc
     5401 gtcagcacgc tgcttgtatt gcttatcaac tataaaatca ctagtcgcat caaaaacatg
     5461 aaaatcaaaa tggccgtgac agcatgcgta tttgcttttt ccatgtttac agtagctggt
     5521 cagctaacga ttttatatca actacctttt ttcttgaatt ggtgggtgat tgctttagga
     5581 gaattggcat caatgattgt cggtgggata attatttatt ggatcggtaa aaaagtggat
     5641 ttgtcaaaat aaagatctgt ttaatttaaa atagggcagc atggaagaga ctgaatgttg
     5701 agtcttcttc catgctgctt ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt
     5761 ttcatcaaga taccgatata agctgctcca gttgccttga ttgcttccgg cagtaaaaac
     5821 ggcagtagtc cactggcaaa tgcagttgta aaagtaagat caccacttat tttcagccag
     5881 atcgttccgc agatcaatgc agcaagagag cccattagat tggcaaagat tgcatataag
     5941 taatgagcat ccgttctttc gatcatccaa ccagttatcc acgtttgtaa gagaaatcca
     6001 atcaaatatc cgccagttgg cccaaataaa acagctatac ctccagtcat ccctgaaaat
     6061 acaggaagac caatcaagcc aagtaaaaga taaactccaa cagatatcaa tcctagtttt
     6121 cttccgagaa aagttgctgt taatccaata gcaaatgttt gccctgtcaa aggaaccaaa
     6181 ccaaaaggaa tggtcatctg agaaaggaca gaaataatcg cggcaaattc cgcagcaagt
     6241 atcaattctt tcaatgttcg gttcataacg ccctcttttc gttaacttaa tatttttatt
     6301 tggttaacga aattgtaaaa caaagtgatt actttgtaaa tagaaaatat aaaaaaagac
     6361 cagctatcca atagatagcg gtctttttca agaaagcttt tgaaattata agctttggtt
     6421 gtagtattca acgacaagag cttcgtcgat ttctggatat agttcgtcac gttctggtaa
     6481 acgagtcaat gaaccttcca atttttcagc gtcaaagctt acgaatgctg ggcgtccaac
     6541 agttgcttct acagcttctt ttactgttac gatgttttgt gatttttcac gaactgagat
     6601 cacttgaccc acttctacgt ggtatgaagg gatatcaacg cgtttgccat caactaaaat
     6661 gtgaccatgg ttaactaatt gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta
     6721 aacaacattg tctaaacgtt gttctagtaa gatcatgaag ttaacaccat gtttaccttc
     6781 tttgattttg ctagctttta cgaacaagtt acggaattga cgttcgttca ttccgtacat
     6841 gtgacgaagt ttttgttttt cagttaattg catgccgtat tctgattgtt tgccacggct
     6901 attaggtcca tgttgacctg gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga
     6961 caatgagatg cctaagcgac gagaaatttt ccatgatggt cctgtataac gagacattaa
     7021 aatttcctcc aatgaatata agtttttgga gtaaaataat ccgttgaaaa attcataatc
     7081 atatagtttg ttcttcaatc ttcacctttg cagccgcggt tacacaatcg aacccgagac
     7141 ggggcaacaa actagggatg agttattatt tttctgctgc attattttac acaaattaca
     7201 gtatatgata gatcatccac gaatgtcaag ataattagta cataaaaacg ctttacaaac
     7261 ctaggctgtc tttataaaat gagagggtat gtgcaaatgg aaaaaagcga aggaagttta
     7321 aggagaaaat caatgaatcc ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa
     7381 aagaaggcga atctttttga aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc
     7441 tgtttgtttt tgactttagg aacggctgtg gcatttgcga tcgcgatgaa gggggaagat
     7501 atcgcgcatg gtctaggaaa gattttatac gcatttatgt tcagctgggg tctggtcatg
     7561 attctataca tgaacgccga attaggaaca tcgaatatgc tttatatgac cgtgggtgtt
     7621 tttcggcaga gacttgcttt aagtaaggca gccaagattc tatttgcctg tatttttttc
     7681 aatatgattg gcggtatatt atgtggattt ctaatttcta tgactacgcc atttcaagat
     7741 ttaacgttgg acaactttat gttagaatca atagcagtaa aactagcaaa accttcagca
     7801 acgattttgg tggaagggat gtttgcgaat atcgttgtca ataccgctgt actgatcagc
     7861 atgcggatga aagaggatgt cggaaaagtg atcacagtcg tattcatcat tttcattttt
     7921 gcttttttag gatatgaaca cgtgatagcg aattttcctg cctttacgct tacttacttt
     7981 gcttctcacg gacagatgga tggaatgaca gtcagcaatg tgttacataa tttgtttttt
     8041 gctttggcag gaaattatat tggtggcggt ttagtaatgg gactgggata tgcttggctg
     8101 aatcagtcaa aatcatccta tgtggactaa aaagtcgttc attaaagggg aagtgccatg
     8161 gaagttttag tttttccaaa aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa
     8221 aatctttttt cctttgctgg agtacctaag ccggaacgcg ctcttttaca acaaccgtta
     8281 ctggatgaga gcaaaaattt atctatttct gaagttttag cttttgttca ttattattat
     8341 gaacaaatcg aaagagagta tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa
     8401 cggcttacat ttgaagactt agtcgtattg ttgccactag ttcagaaaaa agaatggtgg
     8461 gacagcatcg actcttggag aaaagtatac agcacatggt gtaaagccta cccagagcag
     8521 cgtgaagaag tctttcaatt gtttgaaaaa aaggaagatt tttggctacg tcgaatagca
     8581 atcacgttgc aattaggtta taaacagcaa acagacacag gactattgat ccgagcaata
     8641 gacgaagatc gtcaaacaaa ggaattcttt atccaaaaag caattggttg ggcattacgc
     8701 gattatagta agaccgatgc cgattgggta aaaagacagc tgacaacaga tttgagtaag
     8761 ttagctgttc gagaggcaag taagtattta taagaaagcg agacgacaag agatgagacg
     8821 caaattattt gcttttgata ttgatggaac gttgttagat tcaaacaaac aagctttaga
     8881 tagcacaaga gaagcattgg aaaaattacg caaagccggt catctggtca cgattgctac
     8941 agggcggagc cgatttcatg cgcaagagat cattcgcgat cttgatttta cgaactacat
     9001 tttatgtaat ggagcggccg gttttttaga ccatgaacaa gtatataaga atttactaga
     9061 tcgcgaccag ttggatgcgt tcgttcaaga agcgcatcat gagcagatcg atacagcttt
     9121 tgtcagctta gacaatatca agcgttcttc ttccaatgat gtaacggtga tggaagaagc
     9181 gatgcattct ttcggtgctg tcttgcctga attagatcag ttttttgcag aggaacaaga
     9241 aatataccaa gcgttagcat ttttcgatca aacatatgat gggaagttct cttcatatga
     9301 taaaattcga tttgtccgct ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc
     9361 taaagcagcg acaattctgc acttagcaga acaagtaggc atccctcatg aagacatcat
     9421 cagctttggc gatggacaaa acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc
     9481 tatggggaat gctgtgccag aagtacaagc agaagctaag atggtcactg atacaaacga
     9541 tcaggatgga atttggaagg cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa
     9601 aagctgttct gcatcaagta ataagcccag ccaaacaaaa atagctcctc ctatttttcg
     9661 tttttctggg cttattaccg cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt
     9721 gacagaagtg gtcagtttca agaaataaga agggatttac gaaaattgct tctcaaattt
     9781 ttgtaaaatt acggcttatt tctgaagaag tagatgaaac aacgtttatt cgggaaatgg
     9841 gactgtgaca agatgtttgt catagtccca tttttttatt ttatttttca aaactaagaa
     9901 ataccaggaa agagttcctt ttgtttgacc ttttctttct tattgaaaac aactataata
     9961 tagagtgatg ttaaaagaga agaggaattt agtgtgcggg tattgtttat tggtgatgta
    10021 gtaggttcaa tgggacgaga gatgattact gaatatcttc ctagattgaa gaagaagtac
    10081 cgtccgcaag taacgattgt aaatggtgag aatgccgctt ctggacgtgg aatcacagaa
    10141 aaaatctata aaaaattttt gcaagacggc gtagatgtcg tgacgatggg gaatcatacg
    10201 tgggacaacc gcggaatctt tgagtttgta aatgaagcta aaaagatggt tcgtccggca
    10261 aattttccgg aagggacgcc tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa
    10321 ctggcagtga tcaacatgca ggctcgttct tttatggtgg acttggatga tccttttcgt
    10381 aaaatgaatg agctggtaga agaagccaga aaacgtacgc cgatcatttt tgtagatttt
    10441 catggagaaa caacaagtga aaaacaagca atgggctggt ttttagatgg gaaagtttca
    10501 gcagtggtcg gtacacatac acacgttcag acaaatgatg cgcgaatttt gccaagagga
    10561 acagcgtatc tgacagacgt tgggatgaca ggtccctatg atggtatttt gggtatgcgc
    10621 agagaacctg taatcgaaaa gtttctgact gcattaccaa aacgttttga agttgtggaa
    10681 caaggccgat cgattttaag cggctgtatc ttggaattgg atgatacgac aggtcatgca
    10741 aaagaaatcc agctgattca gatcaacgat gatcgaccgt tcatggagta gaaagagagg
    10801 cagttttttg cagcaggagc cggaaattca aaaagaagta aggaaactga caaaactttt
    10861 aagagagaat gaaacgatca ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca
    10921 gtatttagct gagttgagag aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc
    10981 ccactatgac aaaccagcag cagaaaaaga agcaatcaaa caagcggatc aattcatgca
    11041 agaatttgat cagcatccgc ttgttgtagc ttatagaaaa cagctgttgg aagcagatga
    11101 tctgcttcat catttaacaa ctatgataca agaagaaatc aatggacaaa tagaggagga
    11161 aaaacatgcc tcaaaaaact aaaaatactc ctatgatgga acaatatcta agcatcaagg
    11221 cccaatacca agatgctttt ttgttctatc gtttaggaga cttttatgaa ctattttacg
    11281 aagatgcgat caaagcgtca cagcttttgg aattaacgtt gacgagcaga aatcgaaatg
    11341 cagaagaacc tattcctatg tgcggtgtac ctcaccatgc agcacaagga tatatcgatg
    11401 ccttgattga aaaaggctat aaagtagcga tttgcgagca ggtagaagat ccaaagacga
    11461 ctaaagggat ggtcaagcgc gaagtgatcc agctagtcac tcctggtaca gtcatggaca
    11521 gcaaaggatt gtcggcaaaa gacaacaatt ttttaacagc tgtagttagt caaggaaatg
    11581 aatttggctt tgcttatgcc gatttaagta cgggagaatt gaagacggct cggttgcatg
    11641 atgaagaagc tgttttaaat gaagcctctg cattgcagac aaaagaactc gtattaggca
    11701 gtgagatcac tgataccctt cgtgaacagc taagcgggcg tttagggcta gtcttttctc
    11761 aacaaaatga gatggaagaa aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc
    11821 cgctggaaaa agaagcaacc ggcaaactat tgacttattt atcggtgaca caaaagcgga
    11881 gtttagctca tatccaaaaa gcagtagaat accaaccaga tcattttttg aaaatggatt
    11941 attattcaaa attcaaccta gaattaagcc aatctattcg gactgggaaa aagcacggca
    12001 ctcttctatg gttgttagat gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat
    12061 ggctagaccg tccgcttatc caggcaaaac aaattaaagc aagacaagaa atggtcgctt
    12121 ctttgctaga cagctatttt gaacgtatcg atttgcagtc agcattaacg aaagtctatg
    12181 atctggaacg cctagcaggt cgagtagctt tcggtaatgt caatgggcga gacttgatcc
    12241 agctgaaaac ttctttagaa caagtgccaa tgatcagagg gttgattgaa ggaatcgatc
    12301 aaggtcaatg gcaaagtctg ctttcagaaa tgcagccgat ggaccatctc gtccaattga
    12361 tcgaccaagc tatccaagat gatccgccgt tacaaataac ggaaggaaac atcatcaaag
    12421 atggattcaa tgagcagctc gatatgtacc gttctgctat gagaaacgga aagaaatggt
    12481 tggcagaact tgaagcaaaa gagcgtcaag aaacaggaat caagaactta aaaatcggat
    12541 ttaaccgcgt tttcggttat tatatagaaa ttacaaaagc aaatcttgga aatgcagaac
    12601 tggagaagta tgaacgaaaa caaactttag caaatgcaga gcgtttcatc acaccagaac
    12661 taaaagaatt ggagacacaa atcctcgaag cagaagaaaa atctgttgat ttagaatatc
    12721 agttgttttt agctgtgaga gaagaagtca aaaaggctat ccagccgcta caagtacttg
    12781 caaaagcaat cagtgcagta gatgtgctac aaagttttgc aacgatcagc gaacgctatc
    12841 agtatgttcg gccagaactt gtcagtgaca agcatcagct tctgatcaaa gacggacgac
    12901 atcctgtagt agaaaaagta ttaggacatc aggaatatat ccctaacagc gtagaaatgg
    12961 cagaagacga aatgatcttg ctgattacag gaccgaatat gtctggtaaa agtacgtata
    13021 tgcgacagtt ggcattaact gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac
    13081 aagcagtttt acctattttt gatcggattt ttacccgaat tggtgcaagt gacgacttaa
    13141 ttgctggaca aagtactttt atggtagaga tgatggaagc caatcaagcc ctgcgttatg
    13201 ctacgccaaa cagtttgatt ttgtttgacg aactagggcg aggaacagca acctatgacg
    13261 gaatggcgtt ggcccaagca atcatcgaat atatccatcg tcatgtacaa gcaaaaacac
    13321 tcttttctac tcattatcat gaactgacgg tacttgagaa agaacttccg cagcttaaaa
    13381 atgtccatgt tggagccgtt gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg
    13441 atggacctgc ggataaaagc tacggcatac atgtggcaaa gatcgcagga ttgccttcca
    13501 atctactaga acgagcagca gatatcctgc atacgctaga aaatggcgat aacggacacc
    13561 atactgttcc agctgaagta gaagagaaaa ctaaaacagc caaacaggag caaataaaac
    13621 cacctgcaga aaaagtaaat caagaccata tacaagaccg tgtaaaagaa gaaaaccaac
    13681 agctttcttt attcagtgaa ttatccgaaa atgaaacaga agtcattgaa tcactgaagc
    13741 aaataaattt actagaaatg acgccaatgg atgcactaaa cacattatat gaattacaaa
    13801 aacaactgta ggaagaaggc ggatatcgat ggccaaaatc caagaattat ctgaacgttt
    13861 agctaatcag atcgctgcgg gagaagtagt ggagcgacca gcttccgttg tgaaagaatt
    13921 agtggaaaat gcaattgacg ccggcagtac tcagattgat atcttagttg aagaagcggg
    13981 actgcggacg atccaagtga ttgataacgg agaaggcatt ttggcagacg acgtagagaa
    14041 tgccttcaag cggcatgcga caagtaagat ccataatcgc gacgatcttt ttcgcatacg
    14101 aacgctgggc tttcgagggg aagctttgcc aagtattgcc tctgtttctg aaatgactct
    14161 ggaaacggcg aatcaggaag aaaaacaagg cacctatctg tcgctgaaag gcggagaagt
    14221 tattgagcat cgaccagcac cactgcgtca aggaacgaag attaccgtca gcaatctgtt
    14281 tttcaatacg ccagcccgct tgaaatacgt gaaaacactg cacacagaat tagcgaacat
    14341 cggggacatc gtcaatcgac tggcattgag tcatccatcg attgcttttc gtttagtaca
    14401 tgatggaaac aaaatgctgg caactgctgg aaatggcgat ctgaaacaaa caattgcggg
    14461 aatctacgga ttatctaccg caaaaaaaat gctgaaaatc gaaggaaaag atctagattt
    14521 tgagttattt ggctatgttt cattgcctga ggtgactcgt gccagtagga attacttatc
    14581 gacgatcatc aatggacgtt ttatcaagaa cttttccttg aataaagcaa tcgttgcagg
    14641 ttatgggtca aaattaatgg tcggtcgttt ccctattgct gtattggaaa tcaaaatgga
    14701 cccgctttta gtagatgtaa atgttcatcc gacgaagcag gaagtccggc tgtcgaagga
    14761 aaaagaacta acagagctaa ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct
    14821 gattccaagt gcagcggata atctacggtt taagaaaaaa gtacctgatc agcctaaaac
    14881 agaacaaatc gaaatcgatt tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag
    14941 tagtttgaat tttgatcgag agacaggtac ctttttcgta gaagaactaa agaacacatt
    15001 tgttgacaga attgttgata agtctgtgga aaatgttgaa aactattctg gtttttctga
    15061 agaaaacacc acttatccag aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt
    15121 tgataacttt gtagaaaaaa cagtggaaaa atctgaaaag aaagaatttc ccattgtgga
    15181 taacatggag aaaacaaatg aagaacaagc agataaatcc gtagaagagg atgaaaagat
    15241 ttacgaggaa gaattgacac tccatcctga atttgatttc actcaaggaa acgacaaaac
    15301 attagaacaa gccatcaaga aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt
    15361 ggaatatttt ggacagatgc acggaacgta tctttttgcc caaagtagcg atgggttgta
    15421 catcattgat cagcatgctg cccaagagcg cattaagtat gaatatttcc gggaaaaaat
    15481 tggggaagta tctgatgatc tgcaagaatt gcttgttcca tttgtgttag actatccaaa
    15541 cagtgatgct ttgaaattaa aagagcaaaa acacttgcta gaagatgcag ggatttattt
    15601 agaagaattt ggtcaaaaca gctttattgt acgggcccat cctacttggt atcccaatgg
    15661 agaagaagaa tcgattattc gggagatgat cgatatgctg ctgacaactg gaagtgtgag
    15721 tgtaaaaaaa ttccgtgaag caacagcaat catgatgagt tgcaaacgat cgattaaagc
    15781 caaccattat ttgaatgaag cacaagcacg tgtgctgctg aaggatctgg cacgatgcga
    15841 aaatcctttc aattgtcccc atggccgtcc agtattgatt cactttacca attcggatat
    15901 ggaaaggatg ttcaaacgaa tccaagaccc gcattaagtc aaagagaaga gggaaaacat
    15961 gaagattgta ttagcatccc aatcaccaag aagaaaagag ttattggctc atatcgtttc
    16021 agaattcgag attgaacctg ctgatatcga tgaaacgcca tttccagacg aacagcccgt
    16081 cgattatgtt cgtagaatgg ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa
    16141 agaagaccat ttagtgattg ccagtgacac gactgttgtt ctcaatcaag agatcatggg
    16201 taaaccagaa gatctggctg aggctgaatt catgctgaaa aaactaagcg gggaaacaca
    16261 tactgtatat acagctgttg ttttaaaaac aaaggaacga gaagaacgga tactagcaga
    16321 agcacatgtc actttttatc ctttgacaga cgaagagatc aaacgctact tggagacggg
    16381 ggattatgcg gataaggcag gggcatacgg catccaaaat gctgcgagtg tattcgtcaa
    16441 agaaatttct ggagattatt acagtatcgt aggatttccg attggtgctg tcaatcaggc
    16501 actgaaaaca ttcatttaaa caaaaaagcg gactatctga ttttcgattg aaaatccatg
    16561 atagcccgtt ttttttattg aaaagattac ttatctactc tgactaagat tttacttcag
    16621 ctttaccgac tgcttcgccc gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa
    16681 ctttttcatt attggtcaaa gcaacaataa tgtcagtttt cttgccagct tgttctattt
    16741 ctggcaaatc catttcagca atcagggtct ctggtgtgac aaggtatcct tctttcacga
    16801 atacattgaa aggaacaccc tttagttcaa cagtatctaa tcccatgtga acaagtactt
    16861 ccagcccctc attagaaaga ataccaatgg catgttttgt ttcaaagaca ctcgtcactt
    16921 taccgtttac tggtgcatag actttgccgt ttgacggttc gacagcataa ccgtcaccca
    16981 tcattttttg tgagaaaact gggtcattta cttcagttat ttccttgatg gtccctgcag
    17041 cgactgcgaa taatgtttct tttgttgcaa ctgccgtacc agttgctgaa acaggttcga
    17101 aagactgtgg cgttgctgtt gcgccatttg ctgtttgaag ttgtacatca gcagcatttt
    17161 caggaacagc aggatcaatc ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc
    17221 caaatggaat gatgacagca atgatcattg cgatgatgaa aggtacccat gtttctgctt
    17281 ggatagctag tattcctggc aaaccgccta ctccgatcgc gttagctcga acacccatca
    17341 agttagcaaa catgccagct aatccagagc cgaccattgc agcgacgaaa ggatagacgt
    17401 atttcaaatt gattccgaac atagcaggtt cagttacgcc taagtaacaa gaaatcattg
    17461 ctggaatcga gatttgttct tcttttttat tcccacgatg taagaagatg atggctaaga
    17521 ctgcagagcc ttgggcgata tttgataaag caatcatcgg ccataaattc gttgatccga
    17581 aatctgcaat cagctgcata tcgatggcat tggtcatatg gtggagacca gtaatcacta
    17641 aaggagcata caggaatccg aatacggcac taaacaacca gctgatagaa gacgtcaatc
    17701 cagtatttac gatatttgag atccagcttc cgactgtcca gccgatagga cctaaaataa
    17761 cgtgtgcagc taaaacagtt ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg
    17821 actgagggat atatttgcgg aagaagattt ctagatacgc taacataaac cctgccagca
    17881 tggctggaat aacttgcgct tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc
    17941 aaaatggaat atctgctgcg gctgtggaag ctacactata agcatttaat aattgcggag
    18001 aaactaacgt aattcctaaa acgattccta atatttgtgt agtacccatc ttttttgcaa
    18061 tactccaagt gatacctact ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa
    18121 aggcattgac accattccaa aacttcgagt gttcaacgat tgttccacca agactttcaa
    18181 actggatacc ttcaagtaca tttctgaatc caaggatcaa tccaccgaca atgattgcag
    18241 gaatcagtgg tgtaaagatt tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt
    18301 tttgttttgc agctgctttt ccttgttctt tggaaacccc ttcaactcca gatactgcaa
    18361 cgaagtcgtt ataaaaagta gaaacatcgt ttcctatgat gacttggaac tgtcctgcat
    18421 tcgtgaacat cccttttacg ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg
    18481 gatcattcag gacaaaacgc atacgagtcg cacaatgact aactgcagca atattttctt
    18541 ttccgccgat atcatgtaac agcttctcgg catctgcttg atattttgcc atatcaataa
    18601 acccctttct cttaacttgt ctatgcaagt tgtttcttca tttactatac tttttttcgg
    18661 agagaattgc aagcgtttta ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct
    18721 taaaataagc ttttcttaaa aataggaaca ttgaaataca aaaagagctg tagtatagtg
    18781 aagaaatgat ggaagaacga gtgagggaaa aaacatgaat aagttcaatg aaattttttt
    18841 agatttggaa cagaaaattt tagcgaaaga atatcctccg cattcgcttt tacccagtga
    18901 gaaccaattg atcaaaattt atggtgtttc acgagaaaca attagaaaag cgctgaatct
    18961 tttgactacg gaaggttata ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg
    19021 gaatcgcttc gactttccta tttctggatt gactagctat aaagagctac aggagaccca
    19081 acgtattcca agtgaaacga tcatccatac gttagaagaa acagaagtga caaaagcatt
    19141 gaacgagatc actggctggg agatcggtgc gcctgtttgg catttgatac gcgaaagaaa
    19201 aatcaatggc gaagtagtta tattagacac cgattatcta ttaaaggaaa tcgttcctat
    19261 tttaactcca gcacaagcac aaggatcgat ctatgaatat tttgaaaatg agctttcttt
    19321 gacgattgct tacgcgcaaa aggagatcac agtagaagaa gtaacggaaa tcatgaaaac
    19381 agcgatggat cttcacgatg acacttatgc agtagtagtc agaagcctcg ttcatctgga
    19441 agatacccgc tgtttcgagt attctgagtc ggttcaccga ttagataaat tccgtttcgt
    19501 tgagtttgcc cgacgccgaa aagtgtaaat agttcaaaag aaagcaaatt gttggcatct
    19561 gtccactttg gcgctatgat aggatcacaa agggggcatt tttaatggat aaaccaataa
    19621 gaccagaaaa agaagaactg aaagaaaagt tatcacccat ggcttatgca gtcacacaag
    19681 aaaatgcaac agaacgacca tttagcggga aatatgatga tttttacgaa aaggggatct
    19741 atgtagatat cgtcagcggt gaaccgttat tcagctctgc agaaaaatat gatgcaggtt
    19801 gtggctggcc atcattcagc aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt
    19861 tttctcatgg catgcatcga atagaagttc gaagcaaaga agcagattct catctcggac
    19921 atgtgtttac cgatggacca gtagatcaag gtggtctacg ttactgcatc aattctgcct
    19981 cactgaaatt cattccatat gaaaagatgg atgaattagg ttatggggaa ttcaaatcac
    20041 ttgtagaata atggaaatac acaaagagcg gtacaatacg gggatttact cgatcgtccg
    20101 ctcttttttt attcaagcga ttgctatttg aaagaaacgc tccctagact ccttttttca
    20161 caaggtgaaa tgtgctacaa taaagggaac gtatgtgtgc aaaagaaaga ggactagaat
    20221 atgtatgaat atctgacagg tgtggttacc tttatcaacc cctattacat agtagtagaa
    20281 accaatggta tcggctacca gatcgcacta ggaaatcctt accgctacag tagcaaacta
    20341 aatcaaaaaa taaaaattta tgtccatcag gtaatacgtg aagatgcgca tcttctgtat
    20401 ggatttgatt cgctagaaga aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc
    20461 ggtccgaaaa gtgcccttgc aatcatggca agcgaagacc actccggttt gattcaagca
    20521 gtagaatcca gtgatgtcac ttatttgacg aaatttccag gagtagggaa aaagacagca
    20581 caacaaatga tcttggatct aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt
    20641 agtctatttg atgaagcaaa cacaaaagac gctacagctc tatctgaagc aatggaagct
    20701 ctctcggctc ttggttacag tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa
    20761 gtagataatc agacaacgga tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa
    20821 taaggaggag accaaatgac agatgaatac ttgttatcac cagaatctgg cgaaaatgaa
    20881 ctgagtttgg aaaaatctct tcgaccacag ctgctttctc aatatatcgg acagagaaaa
    20941 gtcaaacaag aacttcagat ttacatcgaa gctgccagaa atcgggaaga acctttagat
    21001 catgttctgt tgtacgggcc tcctggatta ggaaaaacga cgatggccat ggtcattgct
    21061 aatgagatga ccgtaaatat tcgtacgacg agtggccctg ctatcgaacg tccgggtgac
    21121 ttagtagcaa tcttgaatga actggaagca ggggatgtcc tgtttatcga tgagatccat
    21181 cgattaccta gagtggtaga agagatgctt tattcagcta tggaagattt ctatgtagat
    21241 atcatggtag gacaagggcc aaccgcacat ccggttcatt tccctcttcc tccctttact
    21301 ttgattggcg cgacgacacg ggcaggtatg ctttctgcac ctttacgaga tcgatttggc
    21361 atcatttctc atatggaata ttatgacgaa caggatctgc aggagatcgt tttacgttct
    21421 tcggatattt ttcaaacaga gatcattgaa gaaggggcat tggaaattgc tcgccgatca
    21481 cgaggaacgc ctagaatcgc taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc
    21541 caaggagacg ggaaaatcga tcgtcctata gcggatcagg cacttacttt gctacaggtc
    21601 gatcaggctg gattggatta tgttgaccag aaattgttgc gaacaatgat cgagttatat
    21661 ggcggcggac cagtagggtt aagtacgctt tcagtaaaca taggagaaga gcgtgagacg
    21721 gtagaagata tgtatgaacc gtatctgatc caaaaaggat ttttaaagcg tacacctaga
    21781 gggcgaattg ctactgctta tgcctatgaa cattttggtt acgactatca tatatgattt
    21841 ttgcttttag attggaggaa aatgaatgag tgatacaaca cgtacgaaac aattgatcaa
    21901 aaacgtattg attgatttgt gccaaacgca atcttttcga aaaatcagcg tccaaaaaat
    21961 cgcaaacgaa gcaaaaatca atcgacaaac attttattat cattttacag ataaatacga
    22021 tttgcttcgt tgggtctatt gtgaggatgc attgcattat ttagatccac aatgtctttc
    22081 acttgataat tgggaagagc aagcattgaa aatgctgaaa gcaattgctg agaagaaaga
    22141 attttatgca aatactgtgc tttctgaccc agaaatttta caaaaagagt tttctcaact
    22201 gatccaaaaa tcatttatca agatttttga acaaatggat gaagaaagac aactttctgc
    22261 aagagacaaa ctattttatg ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa
    22321 ttggatcacg aatgaatatg aagaaacacc gatggaaatc gctatgcagc ttttccgttt
    22381 agccaaggat acggaattat attcttatcg tctttatgca aaagaagagt tggaagaaca
    22441 gtaatagaaa aattttttgt tggaaaagca ataatcgcta aaaggagatg gggaaatgac
    22501 aaaaatttta tttgtttgtt tgggaaatat ttgccgttca ccgatggcag aaggcttgct
    22561 gagaaaacga atagcattag aaggaaggga agcagaattt tttgtggatt ctgctgctac
    22621 aagcacgtat gaagttggaa agacaccgca ccctggaacg aaaaaaatat taaaccaaga
    22681 aaaagtagat atgacgaata tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt
    22741 tgattggatc attggcatgg atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg
    22801 ctcagcgcag gggaaaatcc atttgttttt aagtagcatt cctggaaaag aaaaagaaaa
    22861 tgttcccgac ccatactata ccaataactt tgatgagaca tatcacttga tcacagaagg
    22921 cctgaatcat tggtggaata tttggattga tgaaaataaa ataacaaata atttgtaaag
    22981 ttaacgcttt ctttttaagg cgatgttaga gcatttcctt gtttttttgt aaaatgttag
    23041 gtaaaataaa attaattaag taacactttc ttagctaaag gaggagtgca ttcgatgaac
    23101 gaagagacag tatcgccaat gaagaagctt acgaagttag ttactcatac ttcgtatcaa
    23161 ctttttgatt cttttgtaaa tgacactgaa gaacatgcta agaatgtacg attgcaagga
    23221 aagtttattc ttgagctgca aaaagctgct actgaacata gtctagttat cttacaagta
    23281 agagcaaatc gtttgagtag caagtatgaa acgatctcag gctgggtggc gacaaaaacg
    23341 gtcgaaaaag accgagtgat gatccgccta caaaacgaag agcagcagat ccggatggtt
    23401 cctgtagaac aaattgaaaa aataacaatc ctttcaccag caggggaaca ggaacggtta
    23461 tcaaaataat ttataccaga tagaaaagca cgtatgagct tgtaccatca atcattacta
    23521 atcaaaaata accgaatgct ttaagctgat atcagtaaaa cggaacgttt taggatgagt
    23581 atcatagcat gacagcattc ggttgattag ttgaatcgat tggatggtac aagttttttt
    23641 gataaaaaaa ggtcctgcta gccggggagc tagcaggata aggagttaaa aatgaaaaag
    23701 tgttgttagg gttgtttgtt ggtatgctta tataatagtc attagttgtg aatattttgt
    23761 gacgaaagtt ttcgaaagtt gttaagtttt tgagagtata aaatattttg tgtaaatgaa
    23821 aaaatccata caaaaaagga agtcccttct gtagaataaa gttaacgaca accaattcac
    23881 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
    23941 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
    24001 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
    24061 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
    24121 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
    24181 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
    24241 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
    24301 tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat
    24361 gataagtatg tcgctatttt tatggacgct actta
//
LOCUS       DAZKCZ010000031        24271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000031 DAZKCZ010000000
VERSION     DAZKCZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.031"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..572
                     /locus_tag="ACG8KN_001182"
     CDS             <1..572
                     /locus_tag="ACG8KN_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFP9188252.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="ACG8KN_001183"
     CDS             576..1217
                     /locus_tag="ACG8KN_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188253.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="ACG8KN_001184"
     CDS             1214..2131
                     /locus_tag="ACG8KN_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9188254.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="ACG8KN_001185"
     CDS             2128..2799
                     /locus_tag="ACG8KN_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188255.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="ACG8KN_001186"
     CDS             complement(3340..3828)
                     /locus_tag="ACG8KN_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFP9188256.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="ACG8KN_001187"
     CDS             complement(4029..4295)
                     /locus_tag="ACG8KN_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9188257.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="ACG8KN_001188"
     CDS             complement(4295..4558)
                     /locus_tag="ACG8KN_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9188258.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5079..5219
                     /locus_tag="ACG8KN_001189"
                     /pseudo
     CDS             5079..5219
                     /locus_tag="ACG8KN_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..7184)
                     /locus_tag="ACG8KN_001190"
     CDS             complement(5361..7184)
                     /locus_tag="ACG8KN_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9188259.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="ACG8KN_001191"
     CDS             complement(7782..10487)
                     /locus_tag="ACG8KN_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFP9188260.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="ACG8KN_001192"
     CDS             complement(10793..11740)
                     /locus_tag="ACG8KN_001192"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFP9188261.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="ACG8KN_001193"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="ACG8KN_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9188262.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="ACG8KN_001194"
     CDS             complement(12375..12737)
                     /locus_tag="ACG8KN_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9188263.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACG8KN_001195"
     CDS             complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACG8KN_001195"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9188264.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACG8KN_001196"
     CDS             complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACG8KN_001196"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9188265.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="ACG8KN_001197"
     CDS             complement(14365..15876)
                     /locus_tag="ACG8KN_001197"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9188266.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACG8KN_001198"
     CDS             complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACG8KN_001198"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9188267.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="ACG8KN_001199"
     CDS             complement(17703..18779)
                     /locus_tag="ACG8KN_001199"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9188268.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="ACG8KN_001200"
     CDS             18975..20720
                     /locus_tag="ACG8KN_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFP9188269.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="ACG8KN_001201"
     CDS             complement(21000..21437)
                     /locus_tag="ACG8KN_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9188270.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="ACG8KN_001202"
     CDS             complement(21421..22794)
                     /locus_tag="ACG8KN_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188271.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="ACG8KN_001203"
     CDS             complement(23116..23604)
                     /locus_tag="ACG8KN_001203"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9188272.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="ACG8KN_001204"
     CDS             complement(23626..24228)
                     /locus_tag="ACG8KN_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP9188273.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDFKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6960 a         5163 c         4488 g         7660 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaaaatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DAZKCZ010000032        23705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000032 DAZKCZ010000000
VERSION     DAZKCZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.032"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..120)
                     /locus_tag="ACG8KN_001205"
     CDS             complement(1..120)
                     /locus_tag="ACG8KN_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9188274.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            299..1960
                     /locus_tag="ACG8KN_001206"
     CDS             299..1960
                     /locus_tag="ACG8KN_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9188275.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="ACG8KN_001207"
     CDS             complement(2030..2377)
                     /locus_tag="ACG8KN_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFP9188276.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /gene="opp3b"
                     /locus_tag="ACG8KN_001208"
     CDS             2461..3402
                     /gene="opp3b"
                     /locus_tag="ACG8KN_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9188277.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="ACG8KN_001209"
     CDS             3403..4446
                     /locus_tag="ACG8KN_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188278.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="ACG8KN_001210"
     CDS             4452..5522
                     /locus_tag="ACG8KN_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188279.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="ACG8KN_001211"
     CDS             5512..6465
                     /locus_tag="ACG8KN_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188280.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="ACG8KN_001212"
     CDS             6477..7487
                     /locus_tag="ACG8KN_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9188281.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="ACG8KN_001213"
     CDS             complement(7772..8593)
                     /locus_tag="ACG8KN_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9188282.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="ACG8KN_001214"
     CDS             complement(8690..8968)
                     /locus_tag="ACG8KN_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188283.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="ACG8KN_001215"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="ACG8KN_001215"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9188284.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="ACG8KN_001216"
     CDS             complement(11724..12311)
                     /locus_tag="ACG8KN_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188285.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="ACG8KN_001217"
     CDS             12486..13808
                     /locus_tag="ACG8KN_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9188286.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="ACG8KN_001218"
     CDS             13963..14739
                     /locus_tag="ACG8KN_001218"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9188287.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="ACG8KN_001219"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="ACG8KN_001219"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFP9188288.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="ACG8KN_001220"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="ACG8KN_001220"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9188289.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="ACG8KN_001221"
     CDS             16629..17258
                     /locus_tag="ACG8KN_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188290.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /locus_tag="ACG8KN_001222"
     CDS             complement(17382..18491)
                     /locus_tag="ACG8KN_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188291.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="ACG8KN_001223"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="ACG8KN_001223"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9188292.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19309..19407
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19445..19981
                     /locus_tag="ACG8KN_001224"
     CDS             19445..19981
                     /locus_tag="ACG8KN_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9188293.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="ACG8KN_001225"
     CDS             complement(20021..21784)
                     /locus_tag="ACG8KN_001225"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188294.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="ACG8KN_001226"
     CDS             complement(21787..23502)
                     /locus_tag="ACG8KN_001226"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188295.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7648 a         4330 c         4808 g         6919 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacg
//
LOCUS       DAZKCZ010000033        23685 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000033 DAZKCZ010000000
VERSION     DAZKCZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.033"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1063
                     /locus_tag="ACG8KN_001227"
     CDS             107..1063
                     /locus_tag="ACG8KN_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9188296.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1136..1252
                     /locus_tag="ACG8KN_001228"
     CDS             1136..1252
                     /locus_tag="ACG8KN_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFP9188297.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1307..2989)
                     /gene="recN"
                     /locus_tag="ACG8KN_001229"
     CDS             complement(1307..2989)
                     /gene="recN"
                     /locus_tag="ACG8KN_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9188298.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3007..3456)
                     /locus_tag="ACG8KN_001230"
     CDS             complement(3007..3456)
                     /locus_tag="ACG8KN_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9188299.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3563..4381)
                     /locus_tag="ACG8KN_001231"
     CDS             complement(3563..4381)
                     /locus_tag="ACG8KN_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFP9188300.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4390..5280)
                     /locus_tag="ACG8KN_001232"
     CDS             complement(4390..5280)
                     /locus_tag="ACG8KN_001232"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9188301.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5282..5506)
                     /locus_tag="ACG8KN_001233"
     CDS             complement(5282..5506)
                     /locus_tag="ACG8KN_001233"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9188302.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5511..6848)
                     /gene="xseA"
                     /locus_tag="ACG8KN_001234"
     CDS             complement(5511..6848)
                     /gene="xseA"
                     /locus_tag="ACG8KN_001234"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9188303.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6849..7703)
                     /locus_tag="ACG8KN_001235"
     CDS             complement(6849..7703)
                     /locus_tag="ACG8KN_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFP9188304.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7800..8249)
                     /gene="nusB"
                     /locus_tag="ACG8KN_001236"
     CDS             complement(7800..8249)
                     /gene="nusB"
                     /locus_tag="ACG8KN_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9188305.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8242..8667)
                     /locus_tag="ACG8KN_001237"
     CDS             complement(8242..8667)
                     /locus_tag="ACG8KN_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9188306.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8913..9977)
                     /locus_tag="ACG8KN_001238"
     CDS             complement(8913..9977)
                     /locus_tag="ACG8KN_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFP9188307.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10149..10394)
                     /locus_tag="ACG8KN_001239"
     CDS             complement(10149..10394)
                     /locus_tag="ACG8KN_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9188308.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10533..10907)
                     /locus_tag="ACG8KN_001240"
     CDS             complement(10533..10907)
                     /locus_tag="ACG8KN_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFP9188309.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10990..11904)
                     /locus_tag="ACG8KN_001241"
     CDS             complement(10990..11904)
                     /locus_tag="ACG8KN_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9188310.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11925..12728)
                     /locus_tag="ACG8KN_001242"
     CDS             complement(11925..12728)
                     /locus_tag="ACG8KN_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9188311.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(12761..13750)
                     /locus_tag="ACG8KN_001243"
     CDS             complement(12761..13750)
                     /locus_tag="ACG8KN_001243"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9188312.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(13853..14344)
                     /locus_tag="ACG8KN_001244"
     CDS             complement(13853..14344)
                     /locus_tag="ACG8KN_001244"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9188313.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14535..17342)
                     /locus_tag="ACG8KN_001245"
     CDS             complement(14535..17342)
                     /locus_tag="ACG8KN_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9188314.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(17776..18069)
                     /gene="rpmA"
                     /locus_tag="ACG8KN_001246"
     CDS             complement(17776..18069)
                     /gene="rpmA"
                     /locus_tag="ACG8KN_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9188315.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18082..18426)
                     /locus_tag="ACG8KN_001247"
     CDS             complement(18082..18426)
                     /locus_tag="ACG8KN_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFP9188316.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18453..18761)
                     /gene="rplU"
                     /locus_tag="ACG8KN_001248"
     CDS             complement(18453..18761)
                     /gene="rplU"
                     /locus_tag="ACG8KN_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9188317.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(18781..18857)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            19043..19684
                     /locus_tag="ACG8KN_001249"
     CDS             19043..19684
                     /locus_tag="ACG8KN_001249"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9188318.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(19746..21377)
                     /locus_tag="ACG8KN_001250"
     CDS             complement(19746..21377)
                     /locus_tag="ACG8KN_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFP9188319.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(21500..21859)
                     /locus_tag="ACG8KN_001251"
     CDS             complement(21500..21859)
                     /locus_tag="ACG8KN_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFP9188320.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(22018..22091)
                     /locus_tag="ACG8KN_001252"
     tRNA            complement(22018..22091)
                     /locus_tag="ACG8KN_001252"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22055..22057),aa:Arg,seq:tct)
     gene            complement(22400..23083)
                     /locus_tag="ACG8KN_001253"
     CDS             complement(22400..23083)
                     /locus_tag="ACG8KN_001253"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFP9188321.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(23211..23684)
                     /locus_tag="ACG8KN_001254"
     CDS             complement(23211..23684)
                     /locus_tag="ACG8KN_001254"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9188322.1"
                     /translation="MIFLILILLVVLVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPK
                     NTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIG
                     SPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
BASE COUNT         6877 a         5202 c         3929 g         7677 t
ORIGIN      
        1 ttttatttaa atttatgcag ttatattcta tatcttacaa acacattcca tgtattccaa
       61 ttccctatcc gttataataa aagaaacagg caacaggagg aaaatcatga tcaaatattt
      121 tacattagat aatgagaaac tgcgtaatac cggaaaggaa actgaccaaa cgatctggta
      181 ttgtgtagaa cgtcctaatc aagaagagat tcatcagctg acacatcaat ttgaaattcc
      241 gtcagattac atcacttcca tattggacga tgcagaaaat tcccgtacag aagaattcaa
      301 tcaatcaaag ttgacaaagc cagcgttatt attgttacaa tatccttttg ctaaaacaag
      361 tcctagcggt tattttcaag tggatacata cccgctctct attattttga cgcccaaaaa
      421 gaagcttatc acaattacga atcatgaacc actgttttta aagaaagtct ttagccagac
      481 ctttccaaaa agtgatattt cgcctaatct caatatcttc tttcaattca tttggcaggt
      541 cacactctct tacaacagtt atttagctac gcttcaatct caatgcgata ctttggaagc
      601 caaacttcaa gtatccactg aaaacaaaca actttatcaa atcatggata tccaaaaaag
      661 cctcgtttat ttcagagaag cgacttctgc taatttagat gcattgaaag aactagcgca
      721 aaataaactt ttccaagaaa atcatgcttt gaaaaatcat ttacgtgatg tattggtgga
      781 aacagagcag gcaatgacta cttctcgcat tcaattaaaa ctagtagaac aaatgaatca
      841 gactttttca gcaatcgtat cgaataatct gaataatatt atgaaaattt taacttcttt
      901 aacgattatt ttaactatac cgacaatcgt cggcagtatc tatggaatga acattaagtt
      961 acctattgct gaaagggatg atgcctttat atggctagtt ctattaacat tatttatttg
     1021 tatcgtaact acctattatt taaaaaaagg gaagttttta taaaaaagcc ctcttgtgga
     1081 aatggaaaaa agattgtaca cttgaatcaa ctaattaatg acgaggttga aaacaatgcg
     1141 tgaaaaaaaa caaacaagta cttttgctaa agtaacaaaa tttgtcattt ggacaatgct
     1201 gattttaacg attggatctg ttgtattgac tgctgtaatg agtgtcatgt aaaaataaaa
     1261 tatagctatc agctgctgaa aaggacctga accactgcga ggtggctcag gtcctttttg
     1321 cgagtttcaa tagttctttt gcatgttcga gtgtgagttt tgtaatctca cttccagcca
     1381 gcatacgtgc aatttcttct tctctttctt ttgttttcaa ctctgaaaca gaagtttgcg
     1441 tacgtccatc aatcactgct ttcacaatat aatattgata gtcggcgact gcagcaactt
     1501 gaggcagatg agtgatgcac aaaacttgag agtatttcga tatttgagaa attttatctg
     1561 cgatggcctg tgcaactctt ccgctcacac ctgtatccac ttcatcaaaa ataatacttg
     1621 tcacaccttg ctcagaagaa aaaatagttt tcaaagcaag caacatccga gacagttcgc
     1681 caccagaagc gactttcacc aaaggcttca gtggctctcc tggattggtc gtaatgtaaa
     1741 attcgatttg gtcaaatcca tcatgattca attgtctatt ttcttctttt aagaaacgta
     1801 cctcaaactc agtgttttcc atatacagac ttttcaattc atgcagaatg gatttttcca
     1861 attcttttgc gattttttta cgcgcttcat gaagatctgc ggcttgttgc tgaagtagaa
     1921 gctctttttg ctctagttcc tctttcattt tgtccaattg accttctgaa aaatcagaag
     1981 aagctagctc ttcggtgatt tcgtcatagt aagccaatat tgactcaatg gattcgccgt
     2041 atttacgttt caactgtcgg attaattcta aacgttgcgt aacttcttct aaacgactct
     2101 catctaactc caataaatca atctgacgac tcatatcgcc gattgcatct tgcagcaaat
     2161 agtaagcact ttggatattt tcataaattg cttcatactc cacatctaga tgggcgatcc
     2221 cttgcagttc tgaaacggcc aagccaacac catctacact gttttgttca ttattcgtca
     2281 gtgctccgta acctgcagct aatccatcag caattttttg ataattgcta agtttttctc
     2341 tttcttcttt taatttctct tcttctccga cttgcaaatc agcagcagct atttcttctt
     2401 gctggaaatg gagcatatct attctctgaa cataggattt ttcattttgc tgcacttttc
     2461 gtaccctttt ttccagctca cgataagaga tatattcttg ctgataactc tttttcttct
     2521 gttggaaagc tacatctcca aaacgatcca ataaagcaag atgtgattca ggctggagca
     2581 gttcctgatg ttcgttttgt ccttggatat ctactaaata actgccgatt cttctcagat
     2641 tagctaatgt gataatatga ccattcactc gacaaacatt ttttccagtt aaggacatat
     2701 cccgtcgaac aatcagattg tcctcatctg tttcgatccc taattcaacc atcagttcag
     2761 agaatccttc ttgttttggt agttcaaaca acccttcaag cacacatttt tctgctcctt
     2821 gacgtatata atcgcttgaa cctcttcctc ctgctaacag acctaaagca tcgataataa
     2881 tggatttacc cgcaccagtc tctccggtca gcgcggtcat tccttcgtga aaagaaagcc
     2941 gcaattccgg aataatcgca aagttagtaa tactgatttc ttgtagcatc acattcgctc
     3001 ctcaacttaa aggtaatgca ggaagtcttt cttcaataaa actgcctggt cttcagttcg
     3061 tgtaatcatc aaaacattat cgtcatcatt gataatcgaa aataattctt tcttgtaacg
     3121 tttatcaatc aaatttgctg ctgccgaagc atttcccggc aatgttttca aaataacaaa
     3181 tttttccatt tggtcaactg ccacgaaagc gtcttttaat agtttttcca atttagtatt
     3241 tacatcttcg tttgtttcag ctggtatgct ataacggtag ccgccatctg aagccggtac
     3301 cttgatcagt ttcagttcct ttatatctct ggaaatcgta gcttgagtaa cagatattcc
     3361 tttgcttttc aggatttcaa caaattcttc ctgctttcgg atatcttgct cactcaacaa
     3421 tcgagtaatc aaacgatgcc gatctttttt tctcattacg tcttcgctcc ttagcatatt
     3481 cttcttaatc aatgatagct tatttccttg gtataataaa gaagaatccg catacttctc
     3541 acttgatttt cacagttaca atttatttat ggtcaaactg ttcgtgtgca tttgaaacca
     3601 cttctgccat attaatagct gaattgattg ttccgctttc gggtactttc ctcaagtgag
     3661 ccaagaactc gatatttcct tctccccccg tgatcggaga ataacttaat tccagcacat
     3721 catatccacc ttgtgccgca aatgacaaaa tgtcttctaa aactttttga tgggtctgag
     3781 ggtcacgaac aattccgttt ttccctacag cttctctgcc tgcttcgaat tggggtttga
     3841 ttaaagcaat cacttctccc tcttctttta aaatagcatg taatggaggc agcatcagtt
     3901 ttagtgaaat aaaagaaaca tcgataacag caacttctgg aattcctaag gtaaagtctt
     3961 cgggttttgc ataacgaaaa ttcactcttt ccattacgac gactctagga tcttgtctta
     4021 ttttccaagc tagctgattg tagccaacgt ctaaagcata actcattttt gcgccatttt
     4081 gcaaagctgc atcagtaaat ccgccagtgg atgcaccaat atctaacaat gtttttcctt
     4141 caacagatag atcaaatgct ttgagtgctt tttctaattt taatccccct cgagaaacat
     4201 aaggaagttt tttacctttg actcttaatt cactactcac tggaattttt tctcccggtt
     4261 tatcaaaccg ctcgtttttt tcgttgtaaa tcaaaccagc catcactgaa cgtttggctt
     4321 gttctcttgt ttcaaataat ccttgtttga ccgctaatac atctacgcgt tctttttcca
     4381 ttctttccct catttctcca catcaaattg cttgatcatc tggcgcatca gcccgccatc
     4441 aaaagaaaaa acgtcctctt ctaatttgtc tatgatcttg ttggcggata acagctcaat
     4501 ctccaacgcc tcacgtgtct tttctaggcc taataaacgc gggtaagtat ttttttccat
     4561 acgttcatct ttaccagctg ttttccctaa tgttttcgtt gtaccaatca catcgagtag
     4621 atcatctctt atctgaaaag ctaaacctaa atgttcggct aatcgttgaa gtagcaataa
     4681 gatttcttca ggttgcttcg ctaatatgcc accagccagc aaggcataac gaatcaaatg
     4741 ccctgtcttt ctttcatgaa taaatgctaa ttcttctaaa gaaagctttt tcgattcgcc
     4801 ctcgatatct gcagcttgcc cagcaaccat accttgtgat cccgcacaaa cagctaattg
     4861 ctgaagcagc agaagcttag gagaattacc taagtgtgcc atactgatta gctggaatgc
     4921 tcctgtaagc aaaccatctc cggccaatat agctaatgct tcgccgaata ctttatgatt
     4981 ggtaggtttt cctctacgaa gatcatcatt gtccatcgca ggcaaatcat cgtggatcaa
     5041 ggaatacgta tgcaccattt ctaaagcagc tgctacttga tagacgggaa catcaatagg
     5101 ctcatcaaag gaagcaacgg ccgttaaaag taataaggga cgtattcttt tcccgcctgc
     5161 tttcactgaa taactcatcg catcatagag tcctctatcc gttgtatgct catctaaaaa
     5221 agacaagatt tctttttcta cagcaggtag atgaatagct gaaaagtcag ttaatctcat
     5281 ctcagctttc ctcgtttccg tcaaaatcaa cttcttcatt tgattctgtc atcattttcg
     5341 tcaaggtctc ttctgcttta gctaatgtat cttggcaatg tttgcttaat tccattcctc
     5401 gtttaaacga atctagagct gattctaatg gaacgtctcc ttgttctaac tgcatgacaa
     5461 ttttttctaa ttcttgcagt gactcttcaa aagttggatt ttccatattt ttattctcct
     5521 ttgtctatct cttgtacttg tcctttaacg attccatctt gataatggat cgtcagcaaa
     5581 tccccttgat gaatctcggt gacacttttt actacttgat catttaacgt cgtatagctg
     5641 tagcctcttc ccatgatttt caatggactc aacaaatcaa gagattgaac agcttgagca
     5701 aaacgctgct gctgttgttt catatattgt tggattttct cttctaagcg tttttctaga
     5761 taagcagttt cttggcgagc tgctttaact cgataaagcg gagccacttg ttccaattgg
     5821 tggcttaatg cgatcgtttg tttttctttc gcatgataaa tttgctgtgt agcttgatat
     5881 aatcgctgtt tcaactggtc taatttgatg gtctgacctt catacaatct ttcaggctgc
     5941 cgaaatacat aggaacgtcg agcacgctcg aaccgttctt gtttgcgctg gatctggcgt
     6001 acaaacgcct gctgcaatct agcttgccgt tcttgaatgc gcatcaactc ttctgacaaa
     6061 acaggaacag ctaattctgc agctgctgtt ggagtggcag cccgaacatc tgctactaaa
     6121 tcagcgatcg tggtatctgt ttcatgtcct acagatgaaa tcaccggcgt attagcttga
     6181 taaatcgccc gagcaacccg ctcttcatta aacggccata aatcctcaat cgaaccaccc
     6241 ccacgaccga tgatcattgt atcgaaattg cccatctctt ctactctctg gatattcctg
     6301 acaacattgt ccgctgcttg gtttccttgt acaactgttg ggaacaaaac caattggaca
     6361 attggatatc ttcttttcac cgtcgtgatg atatcgcgga taaccgctcc gcttggactg
     6421 gtaaggaccg cgattcgctt tgggaaacgt ggcagcattt ttttaggccc ttgaaaaagg
     6481 ccttccttca tcagcttttc ttttcgttct tccaattctt gatataaagc acctattcca
     6541 tcaggctcca tgtgctctac atagatttga taagaacctc ccgcttcata taatgaaata
     6601 cgcccaacga caaggacttt cattccttct tttggctgga atcgtaattt ttggaatgct
     6661 cctttgaaca taatagcaga tatttttgca tggtcatctt ttaagctgaa atattgatgg
     6721 gcattggcac gtaaacggaa attcgatatt tcgcctgtta aatatacgcg ttctaaataa
     6781 ggatcggcat caaattttct tttcaaatac ttcgttaatg tagtgacagt taaatattct
     6841 tgtgccatct attcacctgc tccaaaagct tcggcagatt ttacggtttg atacatcagc
     6901 atcgtgatag tcattggccc tacacccttt ggcacaggag tgatccagcc tgcaataggc
     6961 tccacttcat caaatttcac atcaccgatc agcttaccgt tagcatcacg attcatccct
     7021 acatctatca ctacagcacc tggttttacg aattcttttg tgacaaaatg tccacgtcct
     7081 attgctacga ctaaaatatc tgcttcttgt gccagttcag ccagattttc ggttttagaa
     7141 tgagcaacag taacagttgc atctgccatc aagagtagct gagccatcgg tttaccgacg
     7201 atattgcttc gtccgataac tactgctcgt ttacccgccg gatcgataga ataagcttca
     7261 aacattttca tgatgccgta tggagtacaa ggaatcttgt ccggtttacc cgcaaggagt
     7321 ctgcctaaat tcatcggatg aaaaccgtct acgtcttttt cagggcggat agctaacagg
     7381 attttttctt catctatatg ctttggcaat ggtaattgga ccaatatacc atgaaaacta
     7441 ggatcttgat tgtatttttc aatttctgca agtagttcgt cttcagaaat cgtctcagga
     7501 taacgctcta ccaatgaatg gattccgatt tcttttgcta cacgttcttt atttcttaca
     7561 tagatttggc tggctggatt ttcccctacc aataaaacga ctaatccggg atggatacca
     7621 ttatctttca atttttggat tttttcagca atctctgcct gcattttttc agctaattct
     7681 tttccgttaa tcaattctgc catctaacca ctcccagata ttttttgtgt tttcattcta
     7741 ccatagaatt ttcccgattt ataacaggtt tatgatgatt catagaaaaa agctaggact
     7801 tacgccccag cttctaattc tttcaataca ttggataaaa caccgttgac gaatttacgt
     7861 gaacggtcat cactaaatgt ttttgccaac tcgattgctt catttaaagc aacagccgct
     7921 ggtacatctg tcacataagt catttcaaaa atcgcgatac gcaagatcac caaatccatt
     7981 tttgagatcc gattgatgtg ccaattgttt tttaaatgtt tttcaatcac tttatccaat
     8041 tcatcttttt ttgcacagac accgccaact agcgtatcaa gataaacagg aacaaactct
     8101 gattcatctt cgttgatcat gtcgcggtgg tctaattcga ttgcgttgaa aatcgcatct
     8161 tcttttgtta ggtctgcatt gaagtctaat ggaaataaag cctgcaatgc catttcccga
     8221 atctcgtgtc ttgataattc tttattcatt ctcttcctct tcgtcttcaa aaagttcttc
     8281 gattgttggt tgttctaatt tttcaggaac aacagctact acatggatat tgacttcaga
     8341 taaatcgata tccgtcatga aaaggacttg ctggctgacc cgttcctgca tttccattgc
     8401 tactttagga actgaaacac catattctag ataacagtaa agatctactt tcaagccatc
     8461 ttcctcgttt gctaagtaaa caccttttcc gtgagctgca cgtcctaata attcagtgac
     8521 attgctggca aaagttccgc gcattccgta tacgccttct actttagaag ctgcaatccc
     8581 gataattact tcgatgactt caggagcgat cacgatctct cctaaatctt ggtttgcgtt
     8641 taaaatcaaa tttttttcat ctgccattta tttattcctc cctttgccta agcacgagat
     8701 acatccgaat tctctatgat gcggcatagt gatcacgtgg taagaaaact gccgccgatc
     8761 agctgcaaac tgctatgaat tggagaaaac acccaacttt attatctctt acagcttaaa
     8821 agttcttcta tagtgtatca ttaaatcgag tgattttcta taacaaagtc tgtgaacact
     8881 gatttttacg cttacttctt ctattgatga aactataaga tgatcaattc ttttggtgaa
     8941 cgagtcaaca cacgatttcc atcttctgtg atcaacaagt catcctcaat acgtactcca
     9001 ccgattcctg gtaagtagat ccctggttca tcagtgatca catttcctgg tacaaattgc
     9061 ttttctgcac gagaagaaac gtttggtcct tcgtggatct ctaaaccgat cccatgtcct
     9121 gtagaatgtc caaatgcttc cccgtaacca tgttttgaaa tataatctct agcaactgca
     9181 tctaattgga caccagtcat tcctggtttt gcagcatcaa taactgctaa ttgagcatct
     9241 aagactattt gataaatttc ttttaatttt tccccaggat caccaatagc gaatgtacgt
     9301 gtcatatcgg aaacgtaccc ttcgtaatag caaccaaagt ccaatgtgat caagtcacct
     9361 tgttcgatga tttttttgct tgctacacca tgcggcatag ccgaacgcaa tccgctagca
     9421 acgatcgtat caaaagaaac acctgaagcg ccaagagaac gcatataaaa gtctagttga
     9481 tttgcgactt caatctcagt catgcccggt tggatcattt tcaggatgtg atcatatgcc
     9541 atatccgcaa tatgacatgc tttttcgata atagcgattt cttcttcatc tttcacttct
     9601 ctgagttctt cgatcatctg tgaaacaggt actaattccg catcgatgat ttcttctaaa
     9661 accgaatatt ctaaaaatga gactgttgtt tcctcaaaag ctaatgagtg cagttcttct
     9721 ttgcgtacta agtttgctac ctcatcaaaa ataggtccga catttttaat aatttcaaac
     9781 ccttgagcct gcgctgccgc ctgctcggta taacgaaaat cagtaataaa aaatgctttt
     9841 tccaaagtaa taacagctaa tcctgtcgta cctgtaaagt tcgttaagta acgaagattg
     9901 tacggactgg taaccaaaaa tgaatccaaa ttttcttctt gcattttttt gcgcaatttt
     9961 tctacacgta gcatcataat gaaactccct ctttctttgc acgctttttt ccattatagc
    10021 aaacttttct tcgtacacga cagttaaata aaaatttccg gaaaacttct ttttatttta
    10081 aataaataaa gccaaaaagg agtcacagtc tcgctgcaac tccttttagt ttactatttt
    10141 gtaaaaactt atgatcgttt tccaaagaaa aatgagacaa ctgctaccaa aatcactgcg
    10201 cctaaaatag atggaataag tgccatacct gcagcttttg gtccccattc gccgaaaatc
    10261 gcttgtccaa tacttgaacc gactagtcca gcaatgatat tcgttacaca gcccatcgaa
    10321 tttcctttac tggtgatcgc acctgcaatt gctccgatca cggctccaac aatcaatgtc
    10381 cataaccaac tcataatata acctccttct ttgctaatca attttagcat atgggattta
    10441 aaggggacaa ataattggtt ttgctatttc atattttctt ctgcaaagta aaaaaataaa
    10501 gaaaacctcc tcttagaagt tttctttatt ttttacttgc gtttaaacat actacgaata
    10561 ccacgtttca ctacatcaaa gaaactcaat gattgtacca ttctatcttc atctacatat
    10621 tctctgatcg cacgaagcac ttttttaggt tctttggaag aaaatgtgta tgtaccattt
    10681 tttttcgttt gaatggcata tcgaggaatc cattttccct ttagcatgac tgatgctagt
    10741 acataatcca cttcttccca tggaatttgg atatatttgc gtctatctcg cgcatggtaa
    10801 aactcaaaac cattatcacc tatcatgatt tgcccgtaat ctgtcagacc cgtaaacgaa
    10861 gtagcatcaa ttaccaagtc tacttttgta ttgattgatt gaaccatttt ctccctccag
    10921 atatttgttt atgaacagta tactatataa aaaaagctcg ggacaacgcc cgagctatat
    10981 ttttatggat tataaaagtc cgatgacgtg tccaacaaca ccgactacga ataaaccaag
    11041 gatgataacg attggagaaa cctttttctt aagcaaccac atacataaca atgttaatcc
    11101 taatgcagct aaaccaggaa tcaattggtc taagttgtct tgtaatgttg ttaccttttc
    11161 aggagaaagg gctaagccat tattaacttg ttcaaatgca ctcttgattc cttcaccacc
    11221 tgaaggcaat ttatcccatt cgatgtaagc acctttatct aattttacag ttgatacaac
    11281 tggtaagaat ttgattgata cccatctttg tacaagggca gctaatacga acatccctaa
    11341 aattgaagca ccttttgtta cgtcttgtaa caatccgcca gaaaggtcat cagtaatttt
    11401 agaaccagct ttgtagccaa attcttgtgt ataccacatg aatgcccaac gaataatgtt
    11461 ccaacctaag aagaagatga ttggtccaag gatatttcct cccatagcta atgaagcacc
    11521 tagtgcacct agcattggac gtactgtaaa ccagaacact gggtcaccaa caccagctaa
    11581 aggacccatc atacctactt taaccccttg aatcgctaca tcatcaacag gcgcgccatt
    11641 cgcacgttct tcttccagag ccaatgtaac ccctaaaatt ggagaagcaa tgtatgggtg
    11701 tgtattaaag aattccaagt gacgttttaa tgctgcagaa cgttcttctt ttgttttgta
    11761 taattttttg attgctggaa tcatagaaaa tgcccagcca ccgttttgca tacgttcgta
    11821 gttccatgaa ccttgaatga aggtagagcg ccacgcaaca gctaaacgat ctttttttga
    11881 taatttgatt ttttcttctg ccattttatt ttcctccttc aagattaata gtcattcaaa
    11941 atgtcgccta aagggtcacc agagttgctt ccgcctccat tatttgatga accacccatt
    12001 ttagaaaggt tcaagtaaat aagagctaat gcaacaccta aagcacctag agcaatcaat
    12061 gttaattgag aaattgcagc tacaacgaaa ccaataacga agaatggcca tacttctttt
    12121 gtagccatca tgttgattac caaagcgtaa cctacagcta cgaccattcc cccaccgata
    12181 gccataccat ctgttaacca tgctggcatt gattcaagga atgattgcac agtttgtgct
    12241 gggataaata gcaacgctgc tgctgggacc gcgatacgaa ccccttgtaa acatacagca
    12301 atgatgtgcc acatttcaac cttacggatg tttccttctt cagcagccgc atccatcaaa
    12361 tgaacgattg gaacagcgat tgtacgtacg atcattgtta agaacaaccc tgcaacagcc
    12421 aaaggaacgg caatcgcgat cgcagaagga acacctttaa caccttgtcc acctaaaact
    12481 aaaataattg ctgatgctac tgatgctaat gcagcatctg gtgctacagc ggcaccgata
    12541 tttgcccaac ctaaagcgat catctgaagc gtaccgccaa gtacgatacc agcttcgagg
    12601 ttaccggtaa ctaaaccgat caatgtacat gccactagtg gttgatgaaa ttgaaattca
    12661 tcaagaatac cttccatacc agctagaaag gcaactaata cgactaaaat tattgaaata
    12721 atagacatga taagcctcct ataaatttta atattattta ttacgctttt gctaattcag
    12781 cttttgcttt tttcaaaata tcatccatgt tatcgcgtga atcattaggt actttacgta
    12841 catcgaattt cacacctttt tgttcaagct tctcaaacgc ttcaacgtct tcttgtccca
    12901 tagacaatac tttacttaca acgactttac cgaccgaatg cgccattgac ccaacattta
    12961 attctttgat gtctacgcca ccttcaacag ctgtcaatac gtcttcagga ttttcaaata
    13021 aaagcaatgc tttggtattt ccaaaacgag ggtcttttgc tacttcgatc attttgctaa
    13081 taggaataac atttgctttt acacctggag gagcagcttg ttcgattagt ctcttacgca
    13141 gatcatcctt tgatactgca tctgaaacaa cgataatacg gtttggttgc actgcttttg
    13201 tccaagctgt cgcaacttgt ccatgtaaca gacgtgagtc aacacgtgct aatacatatt
    13261 tgatttttcc gtctccgacc acagttcctt ctggaagtgc tcctttaggt tgtgcgtctt
    13321 caactgctgc tttcggtgct tctgccggtt ctaattcttc cggttttact tttacaccgt
    13381 cttttgctgt ttcaatgatg tgtgctgcaa tttcatgagc agattccatt gaaaagcgtg
    13441 atgcgtaggc ttcaatcaac attggtaaat tcaaaccgct gacgattgcc catttatctt
    13501 tgtgctcttc gaataatgtg tttgattgat tgaacggtgt tccgccccat aaatcaacta
    13561 agaataacac ttcgtcttga ttatcaaaag aagcaaccgc ttcttccaat ttggctctca
    13621 agtcatctgg accttcactt ggtttcaaaa tcacggcttt gacgttttct tgttcgccaa
    13681 agatcattga accggattgt aagattcctt cagcaaattg tccatgactt gctaggataa
    13741 tccctaccat atttatacct cctatttact tttgttgtaa caaattcaaa aatattaata
    13801 attgctaacc attacaacag aattatttga ccttttccaa caaccattct tgctacacct
    13861 ttttgatttt cgctagtagt tccatcaaaa ggattttcct gtcagcaggt acttttcgaa
    13921 tctctaactc cacttcttga ctgttcaagt aacgaaagga atcaatatct tgttgattta
    13981 ctgaaacaaa attggtaatc attgatttgc cttcagtaaa actcataccg ccgatattca
    14041 acgacttcaa ctgtacaccg cagcggacaa ccgcttcaac atcttgggga ttggtgaata
    14101 aaagcatcac ttttgctcgt gataataatc cactatgata gatttgaatc attttctgca
    14161 cggttattac atttgctgtg atccccggcg gtgcagcttc ttttagtaga aactttctca
    14221 atttatcttg agcgactaca tcgctgatca caatgatacg ttcaacacct gtttgcttcg
    14281 accaaactgt tgcgacttgc ccgtggatta aacgatcatc tatacgtgcc aatcgaatat
    14341 ccatctgcaa aactccttct tcctcactac ttcacagaaa cccctatcat ttctgtgtga
    14401 acaaccacct cctagcacac actactataa gcaaaaagcg tgccaacttt tttacgatac
    14461 actaattgaa tttaaaggat tcttaaccaa tacacaaaaa cgatacacta taaaagtgta
    14521 tcgtttttgt gtattcatgg cacagtgtat tcttttttat ctaactgatt atggactaga
    14581 cgtagcagat aatacatttc ttctatcggc acttttactt ttatctgatc ttccaacaga
    14641 acaactgagg ctgccaattg gctataaaga ggatcattcg ttgtatttgt caattcgttt
    14701 tcttccacag tcaatggttc attccgaata atacgttcga tcattcctgc tgtgtgcatc
    14761 actaggttga ttttgaatcc atattcttca tcgccgattc ccgtcgtctg agagatttgt
    14821 gtagaaaatt gccacattgg atcgattaac ttactaccat taataaagat gaagttatca
    14881 gagataaact caacacacgt atttttagct gactcttcat ctagggagat gaattcagtt
    14941 tcctccaatt ctgattccat cagcaactga tccaaaatga cttcgatatt ttgatcaata
    15001 aaacgttcta aaggaataaa tggggcttgg atattcaaat cagtaatccc agtagtagct
    15061 atgatctgat attctttttg gataactggc agttcttctt tcacttccac aatcgacaaa
    15121 gctagaactt ctaattctgt ttcttgacgc ttgtttaccg ctcgttcgat caattctttg
    15181 attctttgtg ctgtaccttc gccagatgcg cagatagcca aaacagcttt tttcttatga
    15241 ataggttttg cttcctgaac agattctaca gaagatattt ctgcgtaacc tcgaaaattt
    15301 ttcaatgatt catgcaacat atctagatct gtccctaata ctgagacctt tcgtacagtt
    15361 tccaaaacta gagaagttgt taccatatca acagtacgta ctggaattcc agtgtctctt
    15421 tgaatttgcg aattaaacga cgcaagggac cccatatcta ctaagagtat gaccccgctt
    15481 ccttcatcta cttcttgtac attgcgttca attcggtcca atgctgtttt cggatccata
    15541 tccaaaggca tgtctactgc tcgtacgttc tctgcatcta acaactgttg gacaacttgg
    15601 accatactgc ttgctgtgct gtttccgtga gctgcaacga ctacaccgat tctgccgctt
    15661 gcttgatttg cccgtaacga gacgagtaga acggtcaagt aatagtcttc actttctgga
    15721 atctccactt gaaaatacgt ttcgatataa ctacgcactt ctttcgcaac agcatattca
    15781 tctggaaaat caaaagccat ctcacggatg ttatcattcg tatgccgttc ttctcctaag
    15841 ttgatttttt tcaaaaaaga actgatatgc aagctcatag cataaataaa gttttgctga
    15901 aactcatatg gtaaagatct tttgaccatt acataaatct gattggtcac ttctatgatt
    15961 ttcggatcta caaattctgc taatttgctg tctgcattaa aagaaaaacc atggtcttta
    16021 tagaaagact tcagatggat gttgatatct gtagaaatga aatgattgat tgcttcttga
    16081 tccaaaccat ccgacttcag taaagctgct ttatctccga tgatatcata tagattataa
    16141 ggtaactcat aggagtccga ttggattttc atgatcgtat catttggtgc tacagttatc
    16201 tttggctcca atattttaga aagctctgcc attgcactgc ggttgcttgc taattgaatc
    16261 aatccgctac gaatcccttc tgtcaaatca tcgattgtga tcgagatttc gggtgattgc
    16321 atatgattta gaaatcctcg tgcacatact aactggatat ttgacttcaa ttgtcctata
    16381 tttccatagg tcacactgcc gatcaatgct tttgctacat cttctgtcaa agaaatcttt
    16441 cgctgcgtgc ggtttgcttc gtgggcaatc atgattttta ctaaatcgac tttctcattt
    16501 gcaggacgtt tttcaaaagc cggaagtttg atattgattg ggatacgccg aacaaaagtt
    16561 tctaatagag aagaacctgg gtcttctgtg gtcgcaccta cgatccgtac atttgcttca
    16621 tgggatttcg ttgtttcccc aagacgactg taagttccat gatccataaa gtaaaagatc
    16681 atttcttgac cttcaggcgg caaccggtga atctcatcca aaaacagcat accgccatcc
    16741 gcttgatcga taatccccat tttttcttca tctgcgcctg taaaagcacc ttttgcgtac
    16801 ccaaataaat gactcatcaa aagctcaggg ttattggcat aatcagcaca attgaaaaca
    16861 atcagttccg tttcctcatc tattacatga tgcgcttttg caaaatgata catcgcgtgt
    16921 gcaaaataag tctttcctga tccggtaggt cctgtaatca aacaatttaa tcctcttggc
    16981 ggatacaaaa tcgcagcttt tgcctgttcc actgaatttt tcatgctgcc gttagctcca
    17041 atgatttttg caaagatatc cttagtatct atttggagtg tatttttttc ttcagagacc
    17101 ggcgtttctt tgtaactgac tacatgtttt gtaactggct ttacgtgcgt aacgagtgtt
    17161 tgatatatgt aacgtactgg tcgtccgtct gtctttatca gttttccttc gcgcactagc
    17221 tgattcagat ctttactgct atttgttcgc tgaatcccta acacttcagc aatttcctgt
    17281 gttgttactc cttgatcata ttctgccttt gttaaatgag cggttttttc tttgacatac
    17341 atataaattc ggtcaatgcg ttttgccaat ggtatcgctc cttttctata gactattgta
    17401 cttaggcttc aaaacataat actatgtatt tatttctgtt tcgtcaacta tttacctgat
    17461 tttgttttgt tgataacacg cggatagata gaaatgttca atttaaaaga acgaaaaatc
    17521 ccgatacgat ttttataatg gaaacaagaa aaagaacact gtttaccata taattatttc
    17581 actgatagca aacaatatag cttccatcaa ttgaaaacga tagttatcaa aacatcctga
    17641 ttccaaattg gcaaaaaaat attaagtgta aaaagacaaa gtaaacttcg tagcctccac
    17701 tacaactttt atttttataa acaaaaaagg ctgtgacaaa agtcttgtca caacctcttt
    17761 aatcataaga ttcaattaag cttcttgagc tactggataa acagacactt gttttttgtt
    17821 acggccttta cgttcgaaac gtactacgcc atcaactttt gcaaataaag tgtcgtctcc
    17881 accgataccc acgtttacac ctggatagat ttttgtaccg cgttgacggt aaagaattga
    17941 tccacctgta actgtttgtc catcagcacg tttagcacct agacgttttg attctgagtc
    18001 acggccgtta gatgtagaac cgccaccttt tttgtgggca aataattgta gattcataga
    18061 taacaacatg gtctgcacct cctatttatc attaattgtt ttaatttgga tatattctga
    18121 gttttcagct tctatcgatt gtaatcctaa taaaagattt tctaatagaa tctcagcaat
    18181 attgttttgt tcttgtgtca ttcctgaagt gacttcggta taaagataac cgccttcttc
    18241 ttcattcatt tcaacaatcg gctcaaaacc ggctaatgag gcaatcccgt tgacagtgct
    18301 gatcgctaac gcagaaacac ccgcacaaac gatatcactt ccataaggac cagcatcagc
    18361 atgcccggtc aaagtgaatg aaacgatctg acctgcgtca tttcgcttaa atgttccttt
    18421 gatcatatat aggccttctt tctcaaacgc tattaagcgt tgatcgcgtc gataaccact
    18481 tttgtatatg gttgacgatg accttgttta cggtgtgaat gttttttagg tttgtatttg
    18541 aaagtaacga ctttcttttg tttgccgtgt ttttcaacag taccttcaac agttgcacct
    18601 gcaacagttg gagcgcctac ttttgtagat tctccgccta ctaagataac ttcgtcaaaa
    18661 acaacttttt ctcctgcttc aacgtcaagt ttttcaacat agattgcttg tcccacttca
    18721 acttttactt gtttaccacc agttttaata attgcgtaca tgctataaag cacctccttc
    18781 tgatatactt agactcgcca tccgaagtga cagcatatgc tgtgcttatt acttgttctg
    18841 agcggttgta gctgtggagt ccacaattac aacagtacta gcttaccaga acagcaaccg
    18901 ttagtcaact aaatttaaaa tcaaaaagta aaacaaaaat tcaagcaata cttctgcttt
    18961 tctatcctca cagttctacc aaataaaata gtcgcattgt gtttctttta ttcttataat
    19021 aaaagaaaaa ggaggagcat aaatggaata cccaaaagaa ctgatacaag aagcttcttt
    19081 aagaatgaac ggccgtcctg tagaaggatt tatcgctggg caaggacctc ttcatcctaa
    19141 actgatgctc gtgggagaag ccccagggaa aacagagatt gatacgcatg ttccttttag
    19201 cggtcaagct ggaaaagaat tgatgcaggc actttcttca actggcttaa cacgagaaga
    19261 ggtgtatatc accagtgctg taaggagccg cccgtatcgc gtgactcatc ggatcaataa
    19321 acggacacaa cagtcagaaa ctgtttatcc aaatcggaca cctactcgtt ccgaagtatt
    19381 tgcgcatgca ccaatattgg attacgaact gatgaaggtc cagccaaaat taattgcgac
    19441 acttggaaac attggtcttc aacgtttact tggcaaagaa tatacggtta ccaaaaatca
    19501 tggtcaactc tatacagggc ctgtcttaca attaacagaa caaaaggatg cctacgaatg
    19561 gtcagaaaaa aactatcgaa ttcttccact ttttcatcca gcagctattt tctacaatcg
    19621 ctcccttgta cctgttatcc atcaagactg gcaagtacta gcttcacttt tacagcaata
    19681 ttaaaaagaa cagacaagac cataaaatct tcagtttaca gtcttgtctg ttctttttca
    19741 ttgttttaat ggctaattgc agtatatttc gcatgtaaag ctgtcaacgc atgagcaact
    19801 gtttgatgtt gatgcgtgaa tgtatgttcc cacaagtgag aaagttgttt ttctacttct
    19861 ggctgcaagg aagtatagat aatcgtttgt cctctttgct ctgctttttc ttgaaattca
    19921 tcaaaaactg cacgtgccgt caaatcaata cttggtacgc ctcgcatgga gaaaatgaca
    19981 ccatcgtgat gatttagttc ttccaattta tttttgagtt tttctgaaga cataaaaaac
    20041 agtggaccac taatatacac tactgtccag ttattcagct tttccgtttc tggcagcccc
    20101 attctttgcc aatcgatcgt ttctaatgaa atcgtgatag cagcgctttt gacaacaaaa
    20161 aatacacagc cgccgatcac cccgatcacg atagcaatac ttaaatcaaa gataactgta
    20221 caaatcatcg taaggataaa caatagaact gctgaccaat atttattagt aaatagctcc
    20281 ttgatcgttt cccattcatt catacgccaa gccgtcacaa tcaaaacacc tgcaagtgct
    20341 ggcattggaa tctgtgacat cactggcgca aaaacaagca tggagagcag taagacaact
    20401 gcatgtatca taccagctaa tctagtttgt gcaccagatt tgatggccac gcttgtacgg
    20461 gcgatggccg cagtagcagg aatcccgcca aagaaaggaa gcaacagatt tccaattcct
    20521 tgcgccatta attcttgatt gctatctaag ggcttatttg ccattcggcc agccgaagca
    20581 ccacatagca agctttcaat cattcctaat aaagcaatgc taatagctgg aactgccact
    20641 tgttgcaagg cagaaaagct aaaatctccc atattcaaac gattactact gatcaatgtt
    20701 tgtggaatct tacccacagt tgctactggt aaatggaaca atagcataac tgctgttgcc
    20761 aaaatgatag ccagcagcga actaggcatt cgtttcgccc atttttttgg atagataaac
    20821 atccctaaaa tgactagccc tcccatgatc aaagaggctg tgtttatcgg aaatcctatc
    20881 gtttgataac tacttagttt ttcaataacg tttgtacctt ccgagtgaac accaaataaa
    20941 ttatcgatct gacctaatgc aataatgata gcaatacctg aagtaaaccc cgtaattacg
    21001 ggtgctggga taaaagaagt cagtgttcct aatcggaaaa gtcctgctgc aagtaaaaat
    21061 agacccgcta aaaaagtagc aagcaataca ccttgcattc cctttgttgc cgcaatagac
    21121 atcaagatag ctgccatcgc acctgtcgga cctgatattt gataaaaacc gcctgataaa
    21181 ccgccaataa cgataccggc aatgatagcc gtaatcaaac ctgcggccgc atcagctccg
    21241 cttgataccc caaaagccaa ggctaaaggc aatgctacag cagctactgt gatcccagcc
    21301 aaaagatctt tctgaaatct tgaaagatca taacctgaaa attctttttt tagtgaggaa
    21361 acataatttt tgaacaccta tctaactcct ttttcatgaa attttctaaa ataccattca
    21421 ttagaatatc atatttcatg agataaagta atatttttca caaagtttcc tgttagataa
    21481 attcttattt tttctatatt tataagaaaa tagaaagcaa tgccaacaga gctagcccac
    21541 cttgtttgag taaaatccaa cgatcgctag tcaaactgcc atataatgca acaaaaaaaa
    21601 tagatattaa tagcatactt gtgatttctt tagatgcgga agaaagataa gctccataaa
    21661 gtagtcccac tcctaatagt ccattgtaca ctccttggtt cttaaacaat gtttgaacag
    21721 attttctgct taactcttct ttactcattt taaaaaccct acttgtcgtg tcagaatcag
    21781 ttctcaccgt ttcgatatac atgatataaa aaaattcaat tgcaactaga gtcactaaaa
    21841 ttctagatag tatcgtcata tcttcttcct cttttctgat gagttttatt tttttgctcc
    21901 ttataattgt actcgataag gatgtttttt tattttattt gcctttgaaa acaaacaata
    21961 ttggcacaaa aaacaaagaa aaaccgttag aaacctcagt ttctaacggt tttttagcgt
    22021 cacaggaggg attcgaaccc ccgaccgtcc gcttagaagg cggatgctct atccagctga
    22081 gctactatga caagaatcag cggaacatca ataattatag ataattaaat tgcttctgtc
    22141 aatacgaaat agttatttaa ttagcaaata atgaattatt ttggcccaac tactaaatca
    22201 atcttttaaa acgagacatt ctccttcagc tgttctaagg ttatcaattg aaaacaggag
    22261 ctacattgac ttcatgctac tcctgtttat ttagatttcc tgtatccttt aattgactta
    22321 caaatataga aacttttaca tcaagttcga attgagccct cgattcaatc gattatctac
    22381 aggctgccat aatcatgtat cacaactcga ttgtttctga ttcctttgtc attaagatca
    22441 caccatcacc taatggcaag attgtcgatg tcaaatcggg atgatccatc acaacacgaa
    22501 gaaattcatt taatttacga tggatagctc gtttgccacg gggaatctct tcatctggga
    22561 gcaagatcgt tcctccttgg aagatgtcat ctaccatcaa aacgccacct tttcttaaca
    22621 aacgtaagca ttcaggtaaa aattcaatat attttgattt tgcactatcc ataaaaataa
    22681 agtcatacgg accagacaac tccggcaaga tatctgctgc ttgaccttca agcaaagtca
    22741 ctttatctgt cagtcctaga cgttcatagt tttttttagc tttctcgatc atgacatcga
    22801 aacgatcaat tgtcgtaaca tgagcgtttt ctccgattgt cgtggccatg acacttgatg
    22861 aaaaaccgat agctgctccg atctccaaga ttcgttcagg ctgaagctgt ccaagtaaaa
    22921 atttcaaaaa tacaactgtt tcatggggaa taactggtac ttctgctaca tgagcttctt
    22981 cttccagttt gcctagctca cccggtaatt ttttttgctg ggtacgcata aaatccaata
    23041 gttcttcttt gactacagga cgatgcatca tttcattgcg catactactc ctcctcttca
    23101 cacattatac gaacaactga ctaaatctac aagctgcatg agtgttttct cgtgtttcat
    23161 gttcagaaga gtcgttggat ctctggaata catttatgtt tttaacattc ttacaaaaat
    23221 ttctgtgttc gtctccgctt ctttttcaac tctacaaagg tattccatgc tgtgattcct
    23281 gttaggcaaa aagcaatcgt aaagaataac ccctgaatcc atgcaaatgc cgtttttgaa
    23341 aggactagtc caatatatgg aatctgaaag atgggactac cgataatatt tctctttttt
    23401 actactccag gctcaatata agtctttgta tcagcttttg tataaaaatc ttcgtggttg
    23461 ttttcaacgg ctattacgcg acgcgtcgag attttttttc ctgaattttc ataatatgta
    23521 ataatatctc cgactaaaat ctcatttgta tttttcggtc gtacatatat cagatttcct
    23581 tgtgcataat atggactcat ttctttgtcc tcaataatat aaggagtcaa accaaaaatt
    23641 cggggaacca agacgacgag aagaataaga attaaaaata taatc
//
LOCUS       DAZKCZ010000034        23506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000034 DAZKCZ010000000
VERSION     DAZKCZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.034"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..1547
                     /locus_tag="ACG8KN_001255"
     CDS             120..1547
                     /locus_tag="ACG8KN_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9188323.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQVSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRLTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERTDRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1639..2004)
                     /locus_tag="ACG8KN_001256"
     CDS             complement(1639..2004)
                     /locus_tag="ACG8KN_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9188324.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQNDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2001..2366)
                     /locus_tag="ACG8KN_001257"
     CDS             complement(2001..2366)
                     /locus_tag="ACG8KN_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9188325.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            2804..2932
                     /locus_tag="ACG8KN_001258"
     CDS             2804..2932
                     /locus_tag="ACG8KN_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFP9188326.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2989..4683
                     /locus_tag="ACG8KN_001259"
     CDS             2989..4683
                     /locus_tag="ACG8KN_001259"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFP9188327.1"
                     /translation="MRKSKSIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKSNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4783..5079
                     /locus_tag="ACG8KN_001260"
     CDS             4783..5079
                     /locus_tag="ACG8KN_001260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188328.1"
                     /translation="MQAFEHRLPRGCEKADLHQVIRTPKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKADLHQVIRTPKLKQLLMFLTWLDLLPQMQAFEHRLPRSYEKGV"
     gene            5457..6590
                     /locus_tag="ACG8KN_001261"
     CDS             5457..6590
                     /locus_tag="ACG8KN_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP9188329.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNVLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6621..6911
                     /locus_tag="ACG8KN_001262"
     CDS             6621..6911
                     /locus_tag="ACG8KN_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFP9188330.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKQ"
     gene            complement(6971..7430)
                     /locus_tag="ACG8KN_001263"
                     /pseudo
     CDS             complement(6971..7430)
                     /locus_tag="ACG8KN_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(7560..8813)
                     /locus_tag="ACG8KN_001264"
     CDS             complement(7560..8813)
                     /locus_tag="ACG8KN_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9188331.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8842..9159)
                     /locus_tag="ACG8KN_001265"
     CDS             complement(8842..9159)
                     /locus_tag="ACG8KN_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9188332.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9185..9505)
                     /locus_tag="ACG8KN_001266"
     CDS             complement(9185..9505)
                     /locus_tag="ACG8KN_001266"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9188333.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9970..11385)
                     /locus_tag="ACG8KN_001267"
     CDS             complement(9970..11385)
                     /locus_tag="ACG8KN_001267"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9188334.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREHEERYMDEKGIVQDDYRIEF
                     IGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11401..12717)
                     /locus_tag="ACG8KN_001268"
     CDS             complement(11401..12717)
                     /locus_tag="ACG8KN_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9188335.1"
                     /translation="MRGSGMSFMDKLAKVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISYLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12867..13565)
                     /locus_tag="ACG8KN_001269"
     CDS             complement(12867..13565)
                     /locus_tag="ACG8KN_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9188336.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13854..14847)
                     /locus_tag="ACG8KN_001270"
                     /pseudo
     CDS             complement(13854..14847)
                     /locus_tag="ACG8KN_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(14928..15761)
                     /gene="msrA"
                     /locus_tag="ACG8KN_001271"
     CDS             complement(14928..15761)
                     /gene="msrA"
                     /locus_tag="ACG8KN_001271"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9188337.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15995..16738)
                     /gene="truA"
                     /locus_tag="ACG8KN_001272"
     CDS             complement(15995..16738)
                     /gene="truA"
                     /locus_tag="ACG8KN_001272"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9188338.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(16929..17564)
                     /locus_tag="ACG8KN_001273"
     CDS             complement(16929..17564)
                     /locus_tag="ACG8KN_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP9188339.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(17711..18931)
                     /gene="sstT"
                     /locus_tag="ACG8KN_001274"
     CDS             complement(17711..18931)
                     /gene="sstT"
                     /locus_tag="ACG8KN_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9188340.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            complement(19273..19950)
                     /locus_tag="ACG8KN_001275"
     CDS             complement(19273..19950)
                     /locus_tag="ACG8KN_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFP9188341.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKNVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(20201..20743)
                     /locus_tag="ACG8KN_001276"
     CDS             complement(20201..20743)
                     /locus_tag="ACG8KN_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9188342.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(20748..20975)
                     /locus_tag="ACG8KN_001277"
     CDS             complement(20748..20975)
                     /locus_tag="ACG8KN_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFP9188343.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(21054..22934)
                     /locus_tag="ACG8KN_001278"
     CDS             complement(21054..22934)
                     /locus_tag="ACG8KN_001278"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9188344.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTARVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            23235..>23506
                     /locus_tag="ACG8KN_001279"
     CDS             23235..>23506
                     /locus_tag="ACG8KN_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9188345.1"
                     /translation="MDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLKLLIHT
                     ACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITV"
BASE COUNT         7307 a         4827 c         3960 g         7412 t
ORIGIN      
        1 aaaaaccttt tttaacggca tttatatgct ttttcagaaa ttaactgatg atttcaaaaa
       61 gaaaacatgt cataatattt cccatattgt gcaaaaaaat acaaggcgga ggctctacta
      121 tgattgaaga atatattgag aaaaatattt taagacagct gtttttatgt gggcaattct
      181 atgtaaataa agaagtcaat ctagaaaagc tctccaatct cctgcatgtt tgcaaaacca
      241 ctttgctcaa tgatatcaat aacataaaaa aagaatttga agaacagatc gcttatacac
      301 atcgggaaaa ggaccgttac actttatatt tttcagaaca tatacctcga tgcaagatca
      361 tgcagcaagt gtctcaaaat tcgttgtttt taaaaacttg tctgctttat ttggaagaag
      421 atgaacctga ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc aaagcttatt
      481 cattaaaaaa acaagtatta gcttatttta atgactgcgg gatcgaaatc gaccgctact
      541 cccctcgact cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg tatcgtttag
      601 gcggttttaa ttcttgggag ctacctgaat cgttttttga acgtaccgat cgtttcattg
      661 aatccgtaac ggaaaatagt ggcagatttt atgataaaga aaataaagaa atcttatcga
      721 ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata gattcaaaat
      781 ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt cgaatcagca tgggaaaaca
      841 ctgatttcca aacctattat aaaaaagaag aattcgcctt tattttgaca ctattcaatc
      901 tttgtaatta tggtttccat tcttatcagt tgattgcgga agactttcaa cagctacacc
      961 aagtgtttat tgacaatact cccgaaataa aagaactagt tgctaccttt gaatctcact
     1021 tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc gctgattcat ctgatgcgtt
     1081 cggcttggga taactatcaa ttattcatgc cagaaaaatt ttatctttta aatgaggaac
     1141 agacaaattt atataaagaa gtccagacaa ttttttccag ctggtccagc cagttacctt
     1201 atgatctacg gttgaatcct aattgcatgc gggcttttgt gatcgaattg agcggaattc
     1261 tccgtctgac aaaagaacat ctgactatct atatcgtcac aaattcggat gttcattatc
     1321 tgatttatcg ggaagccttg gaagctgtaa ctactttcga ttttcaagtt gcaccaacaa
     1381 tttattcaag tatatctgat ataaagaagt atgcgcagca gtcctcaaac cgggtattat
     1441 gtgaacgtac attatataca ccagatgcgg tccaatatga aaatattatt cctatttcga
     1501 tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa caaataaggg gctgtgacaa
     1561 aaatcatgtc acagcctctt atttgttgaa gatgcccatt tctgtaacaa tctcttgata
     1621 cacgtcaggc tcacaacctc acttattttg gacatctacg ggtccgatct ctttatttaa
     1681 aaaataagaa atgatggttc ggataactac caacgccgct agttggaaaa tatcctgcaa
     1741 ggtaggtttg ataatcgatt cgatgatccc agctgaaaca agaatttcca aacttagaag
     1801 tatatagcct cccaacatct ttttaatagc attgttctcg ctgactcggt cgttttgcac
     1861 ttttgtatgg acaaataaat atttcaaaaa atccttgatt gatagacaaa caccccaaac
     1921 caataaacag atagcaaaga tatcaagtcc cagtacaagt ttttcaaaat atggaagaag
     1981 aaactgcatc aactctgtcg tcatcctacc tctcctcttc tttgtttttc tttgtcttct
     2041 atttcctgat tcaagaaata agaaatgact gttcgaatga taataataag tgcaagcttc
     2101 aaaatatctg tccaagtcgg cttgataact gactcgatga tatcagcaac gactagtact
     2161 tccaagctta acaaaatata acttgctagc agttttcttg tatctgcatt acgagtatta
     2221 cgttctgcat agtttttctt gacatgagac caatcaaaaa gtttgaatag aacaagaacg
     2281 atcccgacaa ttaagatacc gatagaacaa atatttaaaa tagcaatgat ccattctaga
     2341 aattcaatta aatttgagcg aaacatatac gctacctact ttcttttaat ttgttcctat
     2401 tcttttattg taaaagaaag ttaccttgtt cgtctattta aaagttgttc taatgataac
     2461 ggttcgctta aatttttctt tttcactctc ccatttttct cccctcatcc ataaatcgtg
     2521 gaagtatcca aaacaacgta gcaagttaaa ataaggctta gtccgtcatc agcttttcaa
     2581 agaagcactg tctcggtgct tttttgtacc caaacttcta aaaatagaac catacacctc
     2641 ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag acacttcttc
     2701 ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta ataaaaacga
     2761 actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat
     2821 caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag gaaaaaagac
     2881 aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct agcatcctat
     2941 ttcgttgaat cactacggac atacttgaaa gacattggag gaatcacaat gagaaaaagt
     3001 aaatcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta tttgattcaa
     3061 gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca cggttcaaat
     3121 gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca tccattagaa
     3181 aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac ctatgtagat
     3241 ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac tgctgaagaa
     3301 gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt gatggaaggt
     3361 ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg tattcttctg
     3421 actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat cactatcgaa
     3481 gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat gtgggaaaca
     3541 acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat gcatcaaatg
     3601 atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa
     3661 tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg caaaatcatt
     3721 ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga tgaaatcact
     3781 gtgactggtt tgaaaatgac caatacactg actcaagaag aagaacacgt tgtggtcgat
     3841 gaagatacag cagttatttt cactaatggg tccattacag attcagctac tttaggagat
     3901 tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct ctggaaaaaa
     3961 gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga tcgagatgca
     4021 agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc
     4081 cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac cacaccgatc
     4141 acgccgttag gccaaaaaga tgtcaatatg tctatcgtcg tgcatcatca accgcatttt
     4201 acgtctcaga aatcaaatga aacagttatt tggggttatt tcctttatcc aagacgacgt
     4261 ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct agaagaactg
     4321 atcggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa
     4381 attttcgaca gcgtgataaa taatatccct gtctatatgc cttatgcttc cgcgttgttc
     4441 aataatcgtg ccaaaagcga tcggccaaaa gtcattccta aacattcgac caatctggcc
     4501 tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca aagtgctgta
     4561 cgttctggag aaatcgctgc tttccatttt gcgggtatcc ctatgtcccg tcttgtcaaa
     4621 acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa
     4681 taaccaagag gttgtgaaaa agctgacctg catcaagtaa taagaacaac caggctaaaa
     4741 cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc ttttgaacac
     4801 cgtttaccaa gaggttgtga aaaagctgac ctgcatcaag taataagaac acccaagcta
     4861 aaacagcttc ttatgttttt gacttggttg gacttattac cgcagatgca agcttttgaa
     4921 caccgtttac caagaggtta tgaaaaagct gacctgcatc aagtaataag aacacccaag
     4981 ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat gcaagctttt
     5041 gaacaccgtt taccaagaag ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt
     5101 ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag aaactgatcc
     5161 cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac cattttttca
     5221 acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc aatgattacc
     5281 attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga ctatagaatg
     5341 tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt taaaatgaaa
     5401 ttatctgtag tactaataat gcagacaatt ttatgaattt gaaaggagca tctccaatga
     5461 aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa
     5521 tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc ggctccgatc
     5581 tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta ggacacgaac
     5641 caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg
     5701 tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat cacatggaaa
     5761 gccaatgcga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga tttggcaagt
     5821 tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat gcagactcta
     5881 cttcatttgt tgtaccagat aatgttcttc ctgatgaaaa agttttattt ctatcagata
     5941 ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg
     6001 tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt atggctggag
     6061 cagagcgcgt gatcgcagta gatccagtac agcaccgtct agataaagcg gctaattaca
     6121 atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa ttgtatgaac
     6181 tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga ttagagcctg
     6241 tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct ccattacaga
     6301 tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt tatatgactc
     6361 cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc aagcatggac
     6421 aggcaccaat tatccatcta atgccaaaaa tttacgaaat gatcgcagaa ggactattcg
     6481 atcccacaca aatcattact cattcgctgc cactatcaga agcagcacaa gcatatgaaa
     6541 tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga attcagtaaa
     6601 aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag
     6661 gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc caaaatcgtt
     6721 acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt tttggaagcc
     6781 ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg gctggtgcta
     6841 ttttcttgac aattcgtcct attctcagaa tcgtaaggaa catttttatt tacgacgaaa
     6901 aaaaacaatg aagagaacaa gcaaaaaaaa gaggcatgag atttgaaaaa atctcatgcc
     6961 tcttttttaa ccctatcgta tcactttgat acagacgagc ggtcgtccca ctttcttcct
     7021 tgaccattgt caaaaatgtc cactcgtatc gttcataaaa cgtggtatga tctgtaatta
     7081 aataaagttt ttcataccca agttgatgca ttttttctaa tttccgctaa tatccgcttg
     7141 gctatcccct gcttacgctt ttccggttca acaaacaggg cacaaagatt tggtgtcaga
     7201 tcgggacggt tatggaagtc gttttcaatg acgcccgctc ctccgacgat ggtttctttt
     7261 tcatctaaga ccacatacca ttgaggaata ctttccttat tagcgattcc agtacgaata
     7321 ctttcctcat acacaacagc aggaactccc cattttgatg aaaaccactt cgcactttgt
     7381 tttactaatt ctgggtgttc tcttaatttg atccagttat atgtaaccaa tgtttaaacc
     7441 tctgactaaa ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat tagcaatgac
     7501 aacaatagtg atgtaaaaaa aaacggaaaa caatcacttg ttctccgttt tttgattaat
     7561 caagcatcta aagcttcttt tcttctttct tggatcgtag ctaccttgac gaacggtaga
     7621 tagatcaaaa tagaaagaac caaattgaca gcggctagta caccacctgc aatacttttt
     7681 gttgccaata caccgccaat aattggtgga gttacccatg gaggcataac ggtagctgct
     7741 ggcactaaac ctgtagaagt cgcaaagtaa gcaacactaa ctaagaccat tggtgtcaaa
     7801 ataaatggaa tgaacatgat cgggttcaac acgataggta acccaaacat cgttggttca
     7861 ttaatattga agacacctgg cgcaatcgac aagtttgtta caaccatata tggtttgttt
     7921 ttacgtccta ctaaataaat agctaacagt aaacctaaag ttgcacctgt tcctcctaag
     7981 ttgacaaagc tgtcaaagaa aggtttgttc acgataaacg gtgcgctatg tccagctgcg
     8041 attgcattaa cgtttgcttc aatggctggc gcattgattg tttgcatcaa aggatcgacc
     8101 atattcgctc cgtgtaaacc aaagaaccat aagaatggtg tgataaatgc aagcagtaag
     8161 gcagatggat aagagtttgc aagtcccata aatggttctt ggaccactgt atagaaagaa
     8221 cctacgatat catcaacacc gaatgcagca aaaattgcag caactaaagc aaacgcagag
     8281 atagtgatca ttgctggtag taatgaagca aatgcttttg caactgccgg tggcacacca
     8341 tcaggcattt tgatgatcaa tttttcattt ccgcttaaac gtacaaagat ttctgttgaa
     8401 ataatcccaa caataatagc aataaacaaa ccgtttgagc tcatacctaa tgctccgcct
     8461 aaagcgaaga aagagcctaa agaaatcaca cctgcggaaa caccgtcttt tcggtagccg
     8521 ttagctaaat gataagcaag caagaaagca atcaagacag aaagaatggc aaatgtaccg
     8581 ttccaagcag cagccccgaa tcctttccat gattcaccac caaaaattga gttcatcaat
     8641 tcttggaacc cgggaatcgg taagttattg atcattactg ctaatgctcc taagatcatt
     8701 aacggcatac tcaccataaa tgagtcccga atcgcaacta aatgccgttg tgcaccaatt
     8761 tttgatgcta caggtataaa atgtttctcc ataaatgcaa caaatttatc cataaatctt
     8821 ccctcttttc tttactgtag attacgcgtt caatcgacga tacagatcaa tgatctcttt
     8881 tgctaaatct gtaaaagcta tggatgtcat cagatgatct tgactgtgga ctgtcaaaag
     8941 cgtgacttca acgtgatttc cttgtgcttc ttgtgtcagc atctctgttt gagagtgatg
     9001 tgcttggact aaagattcct ctgcatcttt gattttttca tctgctaaag tgaaatcacc
     9061 ttttttcgca gcagcaatcg cttccattgc atcgcttttt gcatttccgc caaacatgat
     9121 cagacccatg atggcttcta gatttttttg atcttccatt tattgtatcc tccgataatt
     9181 tttattattt gtttattagt ttttctgctt ggtcgagtac tttttcacca ttcatcatgc
     9241 catagtcagc catattgatt atatctaaag gaatgccttt aggtgctagc ttttcagcaa
     9301 attgattttg cataaagcgt acttgcggtc ctaaaagtaa aacgtctaca tttttagatt
     9361 ctagttgatt atccgcatct gaagcagata cagcaaagat gtctgcttcc attccttttg
     9421 cttctgctgc cttttgcatt tttgtgacta ggagacttgt actcattcca gctgaacata
     9481 caagcatgat tgttttcttt tccattttaa acacgttcct ttctatactt acacttgtat
     9541 ataagcaagt agcgtgccaa ctttaaaagc gctttaaaaa gttgatatca tcacatttaa
     9601 ggtctatacc aagttgcata cacactaaaa aagaaagtgc acactaaaca cacaaattaa
     9661 tttttattac cttttttctt ttaacgtgca caaaaaaagt tgtgacacaa aacttgtcac
     9721 aacttttttt cgaataaacg gtattcaaaa gcttgcatct gcggtaataa gtccaaccaa
     9781 gttaaaaaca taagatgttg tttttacttt gctattctta ttacttgatg caggacagct
     9841 ttttcacaac ctctcaataa acggtgttca atcagctgac cctggatttt agctcaaaac
     9901 gctggattca caacctcttt tatttcaaat aaccagtaaa tacaatgaac atctacaaaa
     9961 aacttctatt tatttataga tttcgtcagg caaatccaat tctctcttat caatcaaagt
    10021 ctgataccac gaagcagatt ttttcaatgt tctgcttcgt tcatcgttca attcgattcg
    10081 aactaatcca tatcgattct taaatgcatt cattggtgac acacagtcag tgaatgccca
    10141 taacatatag ccttcgcaat ttgcgccttc ttctgttgct ttgatcaaat aacgcaaatg
    10201 ctgtccgata aattcgatac ggtaatcatc ttgcacgatt cctttctcat ccatataccg
    10261 ttcttcatgt tctcttccca taccgttttc tgttatcagc caagggatat tatgataatt
    10321 ttcttttaga tacatggcta tatcatacat gattttcgga tagatttccc atcccctgga
    10381 attattcatt ttcttcccag gtaaatcaaa gacttcataa tatcgttctg gatggaaagg
    10441 tgtctcttca ttccattgat aacgaggagc gcgaacgcgt ttaggataat attgattgat
    10501 tccaaggaaa tctactgtat gatgctgaat cgtttctaaa tcctctgttg caggatcgaa
    10561 ataaatcgat tgtttttgac acaactccag gaactcttct gtgtagtacc cttttaccat
    10621 cggatcaaaa aatactcgat tataaaacaa atcatacata taagcagctt tttggtcttc
    10681 tggagaatta gatcgagcat agaccatttc aggatttaaa atacagccaa tccttccacc
    10741 ataatttccg ctgcggtaaa tctctactgc tttagccgtt gctaaaactt tatgataatt
    10801 ccacatcatc cacttttttg tgttttgttc atgcggccag cgaacagcat ccaaatagca
    10861 acgcgtttgg ggaacgattg gttcgttgaa tgtaaaccag ttttttaccc tatcaccgta
    10921 acgatcaaag gcaatttgag catacttagc ataaaaatca actacttttc ttgaagacca
    10981 gccatcatat ttttccaaaa gaaaagctgg gacttcataa tgttccaaac aaatcatcgg
    11041 ttctacgtct gcttcgatca aagagtcgat catttcactt atatgctgag catattcttc
    11101 atctactata cagttctcat aatcagtaaa aaaacgagcc caattgatag aagtccgata
    11161 atgtgtcaga tggatggatt tcatcagatc cacatcctgc tggtattgtt ccataaaatt
    11221 agttgctacg gctgggccat acccattatg ccaaacaaaa tgttcatttt tataccagct
    11281 atctataaac gaatcctgtc cttctttttt tcctttccaa ccttccgttt gccaagcaga
    11341 agatgcagca ccaagaataa aattttttgg aatagtaatt ttcatgattt cctcctgttt
    11401 ttagatccca tcattttcca cacgatttgc tacttttaca aatggcagat agatcaaaat
    11461 agaaacaata atacaaacaa gttgcgtaat aactgcaccg atatttcctg ctgttgccag
    11521 ataagcattg acgatcggcg gcattggcca agggaccatt acaactgctt tcgccgcaaa
    11581 ccctaaagcg gttgcaaaat aaccaatcgt tcctgtgata agaggcgtta agataaacgg
    11641 aatagctaaa attggattca gcatgatcgg cataccaaaa ataactggtt cattaatgtt
    11701 gaagatccca ggagcaaggg ataacttggt gatttgtttc atatcctccc gttttccaac
    11761 taacaatact gcaatcagta aaccgatcgt gactccagac ccacccatac tcatatacat
    11821 atcccaaaat ggcatagtaa taatgttcgg tacttctttt ccagcttcaa aagcttccgt
    11881 atttacagca attgccgcta aaagcaacgg ttcccgtatc ggcttcacca tttgatttcc
    11941 atggatacca atcacccaaa agacttgcgc aataaacatc aatagcaaaa tacccggtaa
    12001 cccttgaacg acaccttcta atgggcgttg tacgacattg taaatgatat cataaaggta
    12061 cattcctgta agttctttga tgataaatcc cgcagccgcg atacccgata cagtcagaat
    12121 cgtcgggaat aaagcagaaa aactagcaga aacattcgtt ggcacggtat caggcattct
    12181 cagctgtagt ttcttctgtt tcctcaacca gcaataaagt tcaatagaaa ggatggagac
    12241 caacattccc aaaaataacc ctttcgtgtc tgtatattgt ttagccaata cgttttctac
    12301 tataacagtc tgattttcat tcactaattc ataaacagtt ggattaacag ataaataaga
    12361 catcactgcg atgataccag ggaatacacc atccacttta ttcagcttgg ctaattcgat
    12421 ccctattaga aaaacagcat agatggtcaa gaaacttagt gtgacatagc tgatggcaga
    12481 tgcgataggc tttaattctt ctaaaaaacg caagtaactt atctgtgcta agccattttc
    12541 cgagtcaaag accattgctg aaaacaatgt gccaaaagca cccgtaataa ttaccggaat
    12601 caatgcagca aatgcgtttt ttatcaccat gatatacctt aaattattga ttctcgttgc
    12661 cacgttccct aaaactttgg ctaatttatc cataaaactc attccagatc ccctcattcc
    12721 tgcttcttct ccttgacgtc atctcttttt gtctattagt cagcgcttac attttggtga
    12781 acagaaacta tttactcctc ttttttacca aatataaaac aaaaaatcat ctgatgtatt
    12841 tatcataaaa tacatcagat gaactgtcaa ttagtttctt tttctcctat tattttctgt
    12901 aattctctcc gatttattgc catatggaca gtcattgcgt caaacgcagc aagtacgttc
    12961 cgcctttcga tggcctgaac gatctctgta tgaatatcga tcgtctcgat tttcagatgc
    13021 tggatatcat aatttttatt aaacaaagca atcgattcat taatgattgg gataatatgc
    13081 agcaaggcta tgtttccgct agcttctgca atcatcgtat gaaaatcgat gtctaattga
    13141 acatgttctt gtgtgccttc tcgaaaagaa gtttcaatct tttgcgttaa ttttttcatt
    13201 tggacgattt gattcttcgt tgcatattcc gctgccaaac ctgccatctt aggctccaat
    13261 aaatatctca gttcaaataa gtctcgtatc atccgatcct gatcttcaat caaagaaaaa
    13321 ccgagtgggt cattggcaat ccctcgctta tcactgacaa atgtccccgc tccttgccga
    13381 acttcaagga tattcctgga agctagagcc cgcacagctt ctcgcaccgt gcttcgcccc
    13441 acctctaaat aatcggacaa ttcatattca ttcggcagtt tatctcctac ttgcaactgc
    13501 atttccttta aatatctaag gatcttgtca gtagttactt ctactagggt tttattttct
    13561 tccaaaccac atacctcctt ttcatctgat gtattttttc gtaattatta taacacgttt
    13621 gaatcatctg atgatttttt gtataaaaaa tcgattaatc cgttcataga catacaaacg
    13681 aagaagatat ttaccataaa aaagactagg ataaaattca ctcctagcct tcgatcattt
    13741 ttattttgat cgacaaaaaa tatgatgttc aataacagac gatcttcatt cgattcttga
    13801 tgtcatcttt tgtttttttt aatatatgga taaaaagttt gattacatcg tttctattct
    13861 agaatttggt cgattctcaa ttcttcttct ggtgagaaat ctaaattatc catcattcga
    13921 acactttctt gtaactgact taaccgactt gctccgatca gtacacttgt aacagatttt
    13981 tgtcgtaaat tccatgcaag ggctatttgc gctaatgatt gtcctcgtga tacggcgatt
    14041 gtttgcagtg ctttgatttt ttccagagtt gatccaactt gttcttctga aaggaatggt
    14101 atttccttac gatgtgctct agaatcttca ggaataccat gtagataacg atttgtcagc
    14161 aacccttgag caagcggact gaacgtgatt gcccctagcc cttcttcttc taatactgga
    14221 gatagatcgt cttctagtag tgcacgagaa aacatattgt agcgcatctg atgaataatg
    14281 aacggtgctt cctttctttt taaaatttca gtcatttttt tgtatcttct ccattatagt
    14341 tggatatacc aatatataat gcttttcctt gtcttacaag ttgcatcaac gcttctgctg
    14401 tttcttccaa aggtgtatca gggtctgggc gatgatgata gaagatgtca acataatcta
    14461 gccccattct ttggaaactt tggtcacaac ttgatatgat ggatttacga gagccccatt
    14521 ccccatatgg cccttcccac atatgataac ctgctttgct agaaataatc agttcatcac
    14581 ggtaggcttg aaaatcttct ctaaaaatac gcccaaagtt tttctctgca gctccagcag
    14641 ggcctccata attattcgca aggtcgaaat gggtaattcc catgtcgaat gcgcctctta
    14701 aaatctctct ttgattatgg ataggatcat aatcaccaaa attttgccat aatccgagag
    14761 gagagataaa atcggtaatt tcaaacctga atgaccggat ttacgatatt ccattttttc
    14821 ataacgtgca ctatctgcaa tatacaattt ttctcctcct catcaaatct ttttaaaacg
    14881 ctttcttgtt ttctattttt agtttactac attttaaaga aaaggagtta atcaaaatga
    14941 ttttttcgta tatttttttg ccatttgttt tttactcgtt gatagatcag aaattgatta
    15001 cgtgcttgct ggattttttt atagcgtttc ggttgttttt tgtaaaattg ctgatgatag
    15061 ttttctgctg gccaaaacgt accggctggt tcgatttttg tgacgattgg ttggtggaaa
    15121 gttcctgaac tgtctagttt ctgttttgat tgttccgcta attctttttg tcgttcgtct
    15181 tgataaaaaa tcactggacg atattgcttc cctctatctt gaaattgtcc aaatgtatct
    15241 gtgggatcag taatttgcca atagattgtc agcaactcgc tataacttat ttctcttgtg
    15301 tcataaatga tctccacagc ttccacatga cctgtgtaac caccacttac ttgatcataa
    15361 ttcggttttt caacatgtcc gcccgtgtat cctgacaaaa cagataggat cccttttttc
    15421 gattcaaacg gctcgaccat acaccagaaa catccccctg caaatactgc acgttcttga
    15481 tagtcagcat cttgcatggc atgcttggaa aagtcatatt tttgctcctt atcaaatcct
    15541 tccgtaatca ataaataaaa gtccattaca tcaggcgtca gattcattcg gatagctaat
    15601 ggacgtagtg ttacttctag ttttgataac tgttctttaa cggatatatt ttctcttgtg
    15661 tcattctttg tctgaatcaa tacttttcgt tcccaatctc gtgtattcgg attcaaaatc
    15721 agattataaa gtgtttgaat gaatatttgt tcctcatcca tagattctcc tcctttccga
    15781 agaagacaag ctagtgactt tagctgtaat ttatcataaa gtgaggaaat aatcgattga
    15841 atcgtattta ttcaaagaag ataagcagct tcagatgata taagatgatc tttatcttaa
    15901 aaagcatact ctatggtttg gtttaactaa aaaacgacct ctgaaaaaag ggatgttctc
    15961 caattctcag aagtcgagta aagatgattc ttttttatga gcgataataa acttcatcca
    16021 aaaacagacc ttgtgcaggg acggtttctc ctgcatgttg ccgcactttg ctttcaaata
    16081 tttcatcgat tgtttctatc ggcatggcac ctgtgccgat ttctagtaat gtccccatga
    16141 taatccgcac cattttatat aagaatccgt ctccgacaaa agtaaaatga agcatttctc
    16201 cctttcgttc gatagataat tcatcaatcg tacgcactgt ggattttttt gattttttca
    16261 atgcagaaaa accaatgaaa tcatgtgtac ccactaattt ttcgcaagct gcattcagct
    16321 tgtccatatc caactcttga gggaaataaa agctgtggtt tcgctcaaat gctgaaggaa
    16381 tcacgttgtt ccaaacataa tagctgtatt ttttgcccac cgcattatat cttgaatgga
    16441 agcgctcagg catttcttcg attcttttga tgacaatatc ccgcggaagg taacgattca
    16501 taaagtcgat catttctgcc agtccaattt ctgtgtttgt tttaaaattt gctacttgtc
    16561 ctcgcgcatg ggtgccagca tctgttcttc ctgatccaat aatttctatc tcttcatttg
    16621 tcatctgatg caatactttt tcgatttttc cttgaatggt tttatcgtca tctccaagtc
    16681 tttgccatcc tgaatatctt tttccgtcat attcgattgt caacttgatg tttctcatat
    16741 tttagtggct cctctgtctt ccatttggtc ttctctctac ctttttaaga ttgtagcaca
    16801 aaatatagct ccttgcttct ctaagacaga agctatacaa gaaaactgtg gaaatatata
    16861 tttcagaaaa acaaaaaaca gccaccggat gaagtatccg ctgactgttc aatttgacgg
    16921 ctcaagtttt actgtttgag atgcaagata ctcaaccagg caatgggcac ataattaaag
    16981 aaaaaactga ttctttggca aaaacctatt ttattcagta atggccaaga atttatagaa
    17041 ggaattcttg ccagtccata aatcgccgca aaaagcaaac tgatcacaga cagtccaagg
    17101 tataagtggc ttacgtccat ttgcccattt tttcgataga tcaagaaaag aagaactgga
    17161 aacaataaaa aaccactgta acctaaacct gatccgatat tatggaccca tgtcgagaca
    17221 ttccacgaag attcatgggt attgatactg aacaaacctg taaagataca atcacctata
    17281 ccatagaacg cgatggctgc tgtaactagg gtagctaaca cacgagatgt acttacaaat
    17341 gcagcataca atgcaggcaa tgacaggatg aaaaagatcc ctgaaaccac agaccaaata
    17401 agaaacgctt ttcttacagg actggaaacc tctccaaata cactgatcac gctcttcatt
    17461 tgatctaatt caggataata caatcctaat acgtacggag tcaaaaaatc actcagcacg
    17521 cctaataata aaaagaaaaa accaaattta cgtaaaaaat ccatgatacg cctccaataa
    17581 caattaccta tgcttttaat tgttattata aacgacttta tcttgattta acagaaacct
    17641 ctatttacac gaacatctat ataactcatt aaaaactcga atcattttaa aaatactaaa
    17701 tatctaacgt tcaacgttca cttaatccat ctaaacaatc tttcaatttg attttttccc
    17761 cacttctttt acgcgcgcct agctctccta ttgcagtaaa caacagatca cttgacgaat
    17821 ttaaggcagt ttccattgaa tcctggataa caccgataat aaatccaata ccaacgattt
    17881 gcatcgcgac atcattagag ataccaaaca aactacatgc taatggaatc aataacagtg
    17941 aaccgcccgc aacacctgaa gctccacaag cagctaaagc agacaaaata cataaaagga
    18001 ctttcaaaac aaatggtgct tcaatcccta aagaatgtac agcagtcaac gtcatcaagg
    18061 aaatggtaat cgcggcaccg cccatattga tcgtagcacc taacgggatc gatatggaat
    18121 aagactcttt tgtcaatttc aatctttcag ccaacatcat gtttatcggg atatttgcgg
    18181 cagaacttcg ggtaaagaaa gcgggaagtg cgctttcttt caaacaaaag aaaatcaatg
    18241 gatagggatt ttctttaata aacaaaaacg tgaggaatgg gtacacaact aatgcgacaa
    18301 acagcatcgt accgatcaaa acaagtaaaa gctgcagata ctctgccaat ccagcgattc
    18361 cagtcgtagc aattgattga taaaccaatc ctaaaatccc tatcggagca aatgcaataa
    18421 tcacttgtac gacttgtgac aacgctatag atatctgatc caccaactct tttgtcgatt
    18481 ctgaacgtgc tcgaaaagct agtcccaaca atacagccca gaataaaacg ccaaggtaat
    18541 tcgcatcaat aagagattga acaggatttt gtgtgacatt cgtcaacata gtagataaaa
    18601 cactttccaa actttgcggt gctttttcag atactgtttc ttgtaaagta ataggaacag
    18661 taaaaattga acttacaatg actgctacaa gtgcagaaag caatgttccc actaaatata
    18721 agatcaaaat aggcttcacg aatgtcttat tcccaatttt atgcttggca attgacgcca
    18781 tcgtcaagaa aaaaaccaga ataggtgcga ttgccttcaa gcaactgaca aacaacgtcc
    18841 ccaatattga aataaatgtc cacgtgggga aaaagatacc aaacaatatt ccgactaaaa
    18901 taccaacaac aatacgttga actaacgaca tatccaaaaa cttctctaac aaatgaccac
    18961 cctttcacaa ttttaacaat cattgtatca ttttatctaa agaaactaaa ggtgcaattt
    19021 tctaaaaatt ctaataatac aattggcaat tcatgctctt gcaacattca attttagtat
    19081 tggagggttt attcaatctc taaagaagtc gagagaaaac tatttatcgt tattattata
    19141 gttatccaag taaaacgtaa ctaagatgaa ggacgaaact agaaatttca tccttcatct
    19201 taattcttag acatagcaat caattaatat tttccactga gtctcttctt tctctcactc
    19261 tcactggttt acttacccat aaacgatttg attaaagcca tttttcaatt cattattcgc
    19321 tgtctcgtta aagtaaagat aattatcggg attcccctga ttttgttgtg tcaccaatac
    19381 ttttggctga ttttgctgaa acccgaatag atagacgttc tttttcagat atggttctgt
    19441 ctctgcatcg gaatagacgg ctgtgatttg atagtctgct tgacctgtcc cactttctga
    19501 ccattctgca gaaacaggca ctccaccaat agccatcttc cactccccgt tcaaaataac
    19561 ttgagggact ttaagaccat ataagtccac actcatttga ttcgtatagc ttttgtattg
    19621 ttgtcccatt gtttggcccc atgtagccat aaatgcctct aattgtgcgg atttgtctgc
    19681 attccacagt gtttgttcga cttgttcgct gctttctgaa gatgaagcct ctacagaaga
    19741 acttgaagta gatgtttgag ttgtttcagt cgttttggaa gactctgacg aagtagaggt
    19801 atctgctgtt tccgtacttg ctgcagttgg cttgctctct gtgctagttg ttgtagtagt
    19861 tgcaattgtt gctttcgctg tttcttttgt tgattctttt ttcgattcat tcccgttggt
    19921 acaaccagct aacaaaaaag ctagaccaat tgttactaaa atcctttttt taaattaaaa
    19981 aactcctttc gattactccc acttctttat tctatcaaat aagcaatgtg attttattga
    20041 ttttttgtat tttctcatcc atcttttaaa ccttttaaaa actaagaagc acccgctgat
    20101 gttgtctgta atacatcagc gggtgcttct tttattttaa tcttcgtgat tttccaattt
    20161 acctcacgct ttatatttgt atttgaagaa acatagactt ttagtcatca tcgtcttcct
    20221 catccaaatc ttgtcggaca gtttttcctt ggagtctttg tacttggtag atgatttctt
    20281 tattgaaact taaaagttca acaagcaatt gtttcaagcc atatttttct tctttttcag
    20341 ctaatttgat tgcccgtgtg ataaagacat tttggaaatc aaaatctttt gcaaagtttt
    20401 ccaacattgt tgaggctggt aaatatttgt tttgaccatg ttcgtaaatt tttccataac
    20461 gcatgacttc ttctaaagta gacggaacga tttcattttc aatcactaac tcatgagaga
    20521 ttttattgaa ccatctcgct tcttgatcag catattctga aaaaagggta cttaattctt
    20581 gcgaagatgt acccaaaaca tacaattgtg cttggatcaa tttcatttct tctaatttaa
    20641 aatttgccaa tacatgcccc accatggcgc cagcagttgg tttatgatga tcgatatctg
    20701 cttgttttgt ttcttgagca aatttttctt ccactatatt cactgattta cgcctctttt
    20761 acttttgttg attggacttc ataacctaat ttttccacaa ctgcagctaa ttgttcaggg
    20821 tggacttcat ctggatcaaa gtttgctttg attttgctcg cattaaacaa aactttcgta
    20881 ttgcttaccc caggctgttg agaaaccgca tgttctattt tttgcataca gcttggacaa
    20941 gctagtgtat ctaattgcat aatcattttt ttcatcgtag ttcttccttc ctttttcttg
    21001 atagtcttat tctagattac agcttccata aaaaaattga ttcttatcaa gttttacgat
    21061 ttttcttctt tgctttcgtg tccaaaatac aataaacgca tcgcgttaaa gataacgatc
    21121 aagatactag cttcgtgaac gaacatccca ctagccatgt aaataaaccc ggcaaataag
    21181 ccgatgagta aaaataagac gacaccaatg gcaatcacta cattttcacg ggtattcaaa
    21241 acagttttct tagccaaacg gtaagcatga accaacgatc caaaactaga ttgcatcaag
    21301 acaacgtcac tcgtttcgat agcgacatcc gttcctgatc ccatagcaat cccgatatca
    21361 gccgcagcca aagagggaga atcattgatt ccatctccaa tgaaagcaac gtgcaatcct
    21421 tgttcttggt attttttcac aaattgaacc ttttcatctg gcaataattc agcatggact
    21481 tcatcaatgc ctaacatatc agctacataa tcagcagtca tctgattatc acctgtcagc
    21541 atcactaaat gttttgctcc tgcttgtttc aacatagcta attgttcagc gacttctggt
    21601 cgaatcacat ctgacacacc aatgatttga gtgatttttt tgtcgattgc tacaataatg
    21661 actgagctgc ctctttgttg taattgatgt aaatcttgta cttgctttgg tgtgagagaa
    21721 agtttgtgag cggcaatcaa tttggtgttg cccgcacaaa ctgtatgacc atcaatctca
    21781 gctgtgattc cttgtccctt gatcgtctga ttatctttga ctgctaattg ttgaaaatca
    21841 atgtctttct cctgagcata atggatcacg gcgcgcccca gaggatgatc ggataacgtt
    21901 tctaatcgag ccactaaagc taagctgtcc ccttcatcct ctgtatagtt cttgatagtg
    21961 gatacagcag tagttccttt ggtcaatgta ccggttttat caaagacaaa agtatcgatt
    22021 ttggaaaaag tatccattgc ttcaccgcct ttgactaaga tgccccgttt cgctccatta
    22081 ccgattcctg ctacattcga aacaggcgca ccaatgacta aagcgccagg acaaccaaga
    22141 accaaaacag tgatggctaa acgaaaatca cgactgatca gaccaacaag caacgcaata
    22201 agtaaaacgg ttggagtata atactgagaa aattgatcaa tgaatttttc cgcatgtgat
    22261 ttggtatctt gtgcttcttc gactaattct ataattttgg caaaggtcgt atcatctccg
    22321 accttagttg cttcgatcgt cacgtagcca tctgctaaaa tcgttccaga aaatacgtta
    22381 tctccactga ttttgacgac ttgtcgtgat tcacccgtca ctgcggcttc atccgcatac
    22441 cctttcccgt caatgactcg tccgtccaca ggaatagctc caccagtttt tactaagacg
    22501 atatcgcctt cttccacgtc atcgatttcc acttcttcgg tcgttccatc ttcttggaca
    22561 actctggcag tcgatggggc catctcggtc aaagccttga ttgattcccg cgttttggtc
    22621 aatgtttttt gttccagata actaccaaac aaaaacaaga atgtaacgat agctgactcc
    22681 ttgtattctc cgatcataaa ggcaccaagc acagcaatag taacgagtaa atcaattgag
    22741 ataactttga cttttactgc ctgatacgca tggattataa taggtatgac cgcaataata
    22801 gaagctatgg ctagagcaat gttatagaac aagacaaatc cagtaaattt cccgataaaa
    22861 ccaaaaataa tcaacacagc tgataaggcg gtgatatgat ttcgatgctc ttggatccac
    22921 ttttgtaatt tcatctttct ccctcttttc cttttcattc ttacgagact aagtatagat
    22981 acttatccat cataaaaaat tgaccactat caagtttatt caaaaaggcg gttttctttt
    23041 ggtatactga attcctacag tcattaaacg atcatataga aaaaggaagc gccgaaacgc
    23101 ttccttaggt agttctattt ttttattcaa agataagatg ctttgtcatc ttacgactga
    23161 agaacaacaa tcaaaaaagg gcagcagcca ctcattcaca agaaatatcg ttttttgata
    23221 tgggaattga agcagtggac aaatatctct acttattagc tgggaataag atccaaaaaa
    23281 gcttaatgga ttttatccaa gaattggaat gtactttcca caaaaaattc actcattcca
    23341 ttcttttaaa acttttgatt catacagcgt gtctaattga gcgtacattg ataaacggtc
    23401 atgaacttaa aattatatca gaagatgaca caagaccttc tcatgaaaca atttttcatg
    23461 taaaaaaggc cttcaaaaat attgaaacag aatttgggat cactgt
//
LOCUS       DAZKCZ010000035        23255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000035 DAZKCZ010000000
VERSION     DAZKCZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.035"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8KN_001280"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8KN_001280"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9188346.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG8KN_001281"
     CDS             complement(1172..1867)
                     /locus_tag="ACG8KN_001281"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFP9188347.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACG8KN_001282"
     CDS             complement(1880..2596)
                     /locus_tag="ACG8KN_001282"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFP9188348.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="ACG8KN_001283"
     CDS             2926..3531
                     /locus_tag="ACG8KN_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFP9188349.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="ACG8KN_001284"
     CDS             3856..4179
                     /locus_tag="ACG8KN_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFP9188350.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="ACG8KN_001285"
     CDS             4361..5731
                     /locus_tag="ACG8KN_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFP9188351.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="ACG8KN_001286"
     CDS             complement(5861..7162)
                     /locus_tag="ACG8KN_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9188352.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9383)
                     /locus_tag="ACG8KN_001287"
     CDS             complement(7392..9383)
                     /locus_tag="ACG8KN_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFP9188353.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="ACG8KN_001288"
     CDS             complement(9527..9793)
                     /locus_tag="ACG8KN_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9188354.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="ACG8KN_001289"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="ACG8KN_001289"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9188355.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="ACG8KN_001290"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="ACG8KN_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9188356.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /gene="def"
                     /locus_tag="ACG8KN_001291"
     CDS             13137..13700
                     /gene="def"
                     /locus_tag="ACG8KN_001291"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9188357.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="ACG8KN_001292"
     CDS             complement(13953..14474)
                     /locus_tag="ACG8KN_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFP9188358.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="ACG8KN_001293"
     CDS             complement(14477..15319)
                     /locus_tag="ACG8KN_001293"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFP9188359.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="ACG8KN_001294"
     CDS             complement(15312..16037)
                     /locus_tag="ACG8KN_001294"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFP9188360.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="ACG8KN_001295"
     CDS             16213..16509
                     /locus_tag="ACG8KN_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188361.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16765..16939
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17077..18669
                     /locus_tag="ACG8KN_001296"
     CDS             17077..18669
                     /locus_tag="ACG8KN_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9188362.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="ACG8KN_001297"
     CDS             18682..19422
                     /locus_tag="ACG8KN_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9188363.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="ACG8KN_001298"
     CDS             19590..20939
                     /locus_tag="ACG8KN_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9188364.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="ACG8KN_001299"
     CDS             complement(21067..21657)
                     /locus_tag="ACG8KN_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9188365.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="ACG8KN_001300"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="ACG8KN_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9188366.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6638 a         4698 c         4438 g         7481 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgag
//
LOCUS       DAZKCZ010000036        22300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000036 DAZKCZ010000000
VERSION     DAZKCZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.036"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="ACG8KN_001301"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="ACG8KN_001301"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9188367.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2479)
                     /gene="purK"
                     /locus_tag="ACG8KN_001302"
     CDS             complement(1355..2479)
                     /gene="purK"
                     /locus_tag="ACG8KN_001302"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9188368.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="ACG8KN_001303"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="ACG8KN_001303"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9188369.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="ACG8KN_001304"
     CDS             complement(3376..4683)
                     /locus_tag="ACG8KN_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFP9188370.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="ACG8KN_001305"
     CDS             complement(4686..5267)
                     /locus_tag="ACG8KN_001305"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9188371.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="ACG8KN_001306"
     CDS             complement(5839..7131)
                     /locus_tag="ACG8KN_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9188372.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="ACG8KN_001307"
     CDS             complement(7128..7751)
                     /locus_tag="ACG8KN_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188373.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="ACG8KN_001308"
     CDS             complement(7754..8590)
                     /locus_tag="ACG8KN_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9188374.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="ACG8KN_001309"
     CDS             complement(8607..9494)
                     /locus_tag="ACG8KN_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9188375.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="ACG8KN_001310"
     CDS             complement(9510..10793)
                     /locus_tag="ACG8KN_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9188376.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="ACG8KN_001311"
     CDS             complement(11476..12159)
                     /locus_tag="ACG8KN_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9188377.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="ACG8KN_001312"
     CDS             complement(12177..12794)
                     /locus_tag="ACG8KN_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9188378.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="ACG8KN_001313"
     CDS             complement(12804..13628)
                     /locus_tag="ACG8KN_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFP9188379.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="ACG8KN_001314"
     CDS             complement(13621..14181)
                     /locus_tag="ACG8KN_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188380.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="ACG8KN_001315"
     CDS             14355..15311
                     /locus_tag="ACG8KN_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9188381.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMIHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="ACG8KN_001316"
     CDS             complement(15616..16572)
                     /locus_tag="ACG8KN_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFP9188382.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCERLLTFIRESQEDAREHQENYWLRQPLYYETCFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILARIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16677..17279
                     /locus_tag="ACG8KN_001317"
     CDS             16677..17279
                     /locus_tag="ACG8KN_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9188383.1"
                     /translation="MQFGRFRLGMRTIKSAIAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17367..18866)
                     /locus_tag="ACG8KN_001318"
     CDS             complement(17367..18866)
                     /locus_tag="ACG8KN_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9188384.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMNEE"
     gene            complement(18882..19406)
                     /locus_tag="ACG8KN_001319"
     CDS             complement(18882..19406)
                     /locus_tag="ACG8KN_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9188385.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19581..21230
                     /locus_tag="ACG8KN_001320"
     CDS             19581..21230
                     /locus_tag="ACG8KN_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9188386.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIR
                     YSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYNQEIIGAVCV
                     GLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQLSE
                     KTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGKKISDNLYDVLFKE
                     TIEHQTTVRTQSMYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGTKQ
                     YINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVPAI
                     AGFLLGKINEAKEQSVEMLLETDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQKHE
                     RNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLIHS
                     IVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21199..21915
                     /locus_tag="ACG8KN_001321"
     CDS             21199..21915
                     /locus_tag="ACG8KN_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9188387.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     LKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22017..>22300)
                     /locus_tag="ACG8KN_001322"
     CDS             complement(22017..>22300)
                     /locus_tag="ACG8KN_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HFP9188388.1"
                     /translation="LKSNHLAGFATDVFANETEVFFRSFKPWETIPDPAIQQLVEMYP
                     RVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPKARQLR"
BASE COUNT         6567 a         4806 c         3904 g         7023 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gcactgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatcagtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatga tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagt
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agatgcaagc
    15421 ttttgaacac cgtttaagga agaggtcgtg agcctgccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaactcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgctaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gcaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 acgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actacgatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc cccttttttt attttaatta tacttgaata atgaaaaact
    16621 agctatcaga caaaagttta ggtatgatag taaaaagacc aaaaaggaga atctctatgc
    16681 aatttggccg ttttcgacta ggtatgcgta cgatcaaaag tgccatagct gtttttttat
    16741 gtatcttgtt ttttcatttg actcaacgag gaatcccgat gattgctgca ttatctgctg
    16801 ttttttcttt gcgtcaggac ttgactacaa ccgtttcttt tggaaagtct aggatcatcg
    16861 gcaatacatt aggtggtaca ttagctatca tttatttttt tgtcaaaagc tattttcaca
    16921 atgatttttt ggtagaattg atcttgcttc ctattctagt tattattgtg atcgtcgtgt
    16981 cagatggtat taacaataat tccggtatca tttccgcaat cgctacgttg ttgctgattg
    17041 cgttgagcat ccctcaaggc gaatccgctt tgtatgctat ccaacgtgta ctagatactt
    17101 ttatagggac attcattgca atcggtatca atttctttct tcgtcctcct gaaacagaaa
    17161 agaaggaaga actatcagaa gatttggtcg aactccaaaa gaaagaacga ttgctaagaa
    17221 caaatttaca agagatagaa gctaagatcc aaaaacaaaa gaaaaatagc gaggactaat
    17281 tttcttttgc aataaaataa aaaaatggag agaagaactt taaaaagtct tcctccattt
    17341 ttgattgaac ggtgaagtgt actattttat tcttcattca tcgagttttg ttctgcctct
    17401 tttggcgctt catacatatt tacttggcta gtagtcccac gtgtcgacaa attctctacg
    17461 atttccttca agtcaagtcc tgttgtcgct tttagtgttt cttgagtaga agaaagcaaa
    17521 tttgtggcat aatttgtcac tcggttcgct ccactggttt ctcctgaact gcttcctgta
    17581 tcgacaacag agattttttc gatatttcct agcggctgtg ctgcttcgcg catcaattgc
    17641 ggtagcatat cgataaccat actcaagaca gctgcttcgc catattcctt gaaggcattg
    17701 gcgatctttt gttttgcttc ggcttcggct tttccttttg ctagtgctgc ttcggcttct
    17761 gcttgtccag ctaaacgtgt cttattggct tccgcttctg ccagtgcttc cacacggaat
    17821 cgttctgctt cggcttcagt cacttctttg actttttgag cttgtgcttc ttgttcttta
    17881 gcgtaccgat cggcatctgc ttttttcttc acttccgaat catattgctt ttcacgacgt
    17941 gtgatttctt tttcttccag ttcgatttgt ttttgacgtt ctacgatttt aatctgcatt
    18001 tcctgttcga ttacttgttg ttgtgctcgg gcactttcta aattgtaggc ttggtctgct
    18061 tttgctttag caacatcttg ttcttgtttg taggttgcta atttcagttc tttttctttt
    18121 aatgattctg cgatctctgt ttgtcgctgt aattcagctg cttgtgattc tttttctgct
    18181 tgagcttttt tgatacgcgt ttcttttaga gcttccgctt ctgctatatc tgcatctctt
    18241 ttgacttggg cgattcttgg tttccctaaa gagtcaagat aaccgttttt gtctctgacc
    18301 tctttgatag tgaaagaaac gatgatcaac cccatttttg caaggtctac agaagcgact
    18361 tcttgtacgc tttgactgaa cttatcgcga ttttgataga tttcttctac agtcatagaa
    18421 ccaagaatcg agcgcaagtg tccttctaat acttctcttg cttcattttc cagttcttct
    18481 cttgtcttgc ctaaaaactg ttctgctgct gtagcaatct cttctacaga tgaaccgatt
    18541 ttgatgatcg aagtaccatc acacatcacc ggtacgcctt gttccgtata cacttcaggg
    18601 gtggagacgt ccaatttact cgataataga ctgatccgat tcgaacgttg aaaaactggc
    18661 aagacgaatg cgccacctcc acgcacaatt ttgattttgt tattgctttc atcaatgtgc
    18721 acatttttgc ttcctaaata acttccactg atgatcaacg cttcatccgg ttttgctgtc
    18781 tggtacttcg ctacaaagac gatcagcaac atcagtaaaa tcaaagctgc gattgcgatc
    18841 gcgataaaaa caactggtat ttccatttaa cacacgctcc tttaagcttt tatcatgctt
    18901 tcataaggca tgacataaca gattctttcc ttgatctcaa tgatcaatac tttcgttccc
    18961 cgctcagctg tctgttcttg ggggctgtaa aaacaagctg gacgactaat ggaaccggta
    19021 acacttttca actgaatttc tcccatacct cgtacaggga tcggtgtgat cacaacagct
    19081 acttgtcctt ccagagtttt ttcagaagac gacatcgttg attcggcatg tttcatcggc
    19141 atcaatatat agaaattaag caaaaatacc aacactatcg atatgcctcc actagcaata
    19201 aataaaaata aagatgattg atctgttaaa cgctcaccta gaaaaccaaa caagcttgtg
    19261 aaagttaacc aagggataat aagcatcgga tcaagcgggc catcaaaatc gaatacatct
    19321 ccaaaaataa tcaataggac tgccaatcct gcacaaatca gcaatgtata ccaataaatc
    19381 gtctcaagtg cgattccccc tatcatatac acgcctcctt ccatttctac gtctatcata
    19441 ccaatgtttg aaaattctgt atagcttctt ttaccgactc agtggtgttt tttataggaa
    19501 ggttgtgttt atcgttgaca atttgttaaa tttttgtttt catgttaaaa ttttcacaat
    19561 aaattctttc gaggtagcct atgtttcctt tttttaagaa aaaaccatta aagctccaga
    19621 acacgatcat ccttgtcgtt tgtttctcca cattcgtttc tttgattgtt tctgccatat
    19681 tgatcaggaa ttttattctt tcgatgaacc gatctagtgt gtcagataaa atctctagca
    19741 ttgctaaaat cgttgccaat gataaactca tccaaaaaaa gctgcagact ggtgacccaa
    19801 cacatgcgat tcaaggaata acaaatgaaa tcatgattgc atcgaattta gattttatcg
    19861 tggtcatgga ccctgaaggc attcgatatt ctcacgcaga cccaagttac atcggtcttc
    19921 ctttctcaag tcatgaagat gtgaaaaaag tgattggagg acaagaattt ttctctgaaa
    19981 aagaaggaat gttagggttc ggcttccgct atttcgttcc tatttattac aatcaagaga
    20041 ttattggtgc agtttgtgtt ggcttaacga acaacacgat tgacaatcaa gtcaaagatg
    20101 gacaaaaaat gattgttttt tcattgatta tcggacttag cgtaggcatc ataggcgccg
    20161 tttttcttgc ccaaaaaatg aaaaaagtac tgcttggact tgaaccacaa gaaatagcag
    20221 aacagttaag tgaaaaaacg attatcgaaa atgaagtaga tgaaggaatc ttagccatct
    20281 cttctgataa aactatcctc ttgatcaata cacctgcaaa aaacattttc taccaaacag
    20341 agcagctatt ggatttcgaa gtaggaaaaa aaatcagtga taatctatat gacgttttgt
    20401 ttaaagagac gattgagcat caaacgaccg ttcggactca atccatgtat ttaaatggga
    20461 tagaaactat ttctagcgtt acacctcttt ttagcaatga ccgtttttta ggagcagtcg
    20521 caactttacg aaatcgatca gaagtaatga aattaatgga ggagcttggg gggacgaaac
    20581 aatatataaa ctccatgaga gctcaaaccc atgaattcat gaataaactt catgtcattt
    20641 ctggtttgat tgatttgaag aagtatgaag aagtcaccca ttttatccag cagctaaatc
    20701 aaaattataa aaaagagatt ggccaagtct caagtctgat caaagttcct gcaattgccg
    20761 gttttttatt aggcaaaata aacgaagcca aagaacaatc tgttgaaatg ctgttagaaa
    20821 ccgattcttt tatccctaaa atggagatgg atgactccat ccattcactt ttgcaagtgt
    20881 tagggaactt acttgataat gcaaaagaag ccgtgctaca aaaacatgaa agaaacggac
    20941 aggtccggat tcatttaaac tatgacacag aagctgaagt attcatcgtg accgtctgcg
    21001 atgacggtgt cgggatatca gaagaaatct cagaaaaaat atttacgcta ggtttttcga
    21061 cgaagggaat taatcgtggg tttgggttga atctcatcca ttcaatcgtc aaacgccacg
    21121 gtggattttt agaattttcc aaacaaacaa atggcggtac aatcgtctat ctagaattac
    21181 caaaaaagga agatcaaaat gatgacaaat gtcctgataa tcgaagatga tccaatggtt
    21241 gcagaattga atcgtacttt tgtggaaagc atccctaata tgaacgtaat cgaaagtttc
    21301 agaaatgtag aagaaggaaa aaaatatttc agacaaaaaa acgcagatct gctgctgctt
    21361 gatgtataca tgcctggcga aagtggtatc cattttttat cttggcttag acagcaagga
    21421 aatgatatcc cagtgatttt gatcactgct gcagatgata tcgaaaccat aaagaatgtg
    21481 atccgctacg gtgccttaga ttatttgatc aaaccgttca ctttgcaacg ttttcagctg
    21541 gcgatcgaaa agtatcttgc tttctctcag acaatccaaa cgactgaaaa agccgatcag
    21601 aaattgatcg atcaagtatt caaccacacc actcccattc acgaaatacc atcattcatc
    21661 gatttgccta aaggtctgtt aaaactgact ttaaaaaaaa taagtgcatt tattctaaaa
    21721 gaagatacta ttttttcaac aaaagaactg gctcaaaaaa gctcactgtc acgtatatca
    21781 gtcaagaaat atttgaattt tttggtagaa caagatatat tgaacgaaag aatcaaatat
    21841 ttggaaatcg gccggccgct aacctgttat gaattgacaa agaatgggcg gaaaaagctc
    21901 acagattact gttgagtcat cgaattatcc catacaaaat agtccaagta tacagaagta
    21961 acaactgttt acttggacta tttttaaaca ttttattgac gaatatccat gttttcttat
    22021 cttaattgac gggctttagg aagagatacc tcatttttcg tttttccagt ttctattatt
    22081 tcacgaaagt tctcgaaact tgtcgaaatc atatttgata atgcctcatc tgtatttgaa
    22141 cctacatgcg gggttacaag aactctaggg tacatttcta ctaattgctg gatagcaggg
    22201 tctggaatcg tctcccatgg cttgaaggat cggaaaaaaa cctctgtttc atttgcaaaa
    22261 acatcggttg caaaaccagc taaatgattg cttttcaaag
//
LOCUS       DAZKCZ010000037        21594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000037 DAZKCZ010000000
VERSION     DAZKCZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.037"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..404
                     /locus_tag="ACG8KN_001323"
     CDS             177..404
                     /locus_tag="ACG8KN_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9188389.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            420..1586
                     /gene="hemW"
                     /locus_tag="ACG8KN_001324"
     CDS             420..1586
                     /gene="hemW"
                     /locus_tag="ACG8KN_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP9188390.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1821..2864
                     /gene="hrcA"
                     /locus_tag="ACG8KN_001325"
     CDS             1821..2864
                     /gene="hrcA"
                     /locus_tag="ACG8KN_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFP9188391.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2958..3521
                     /gene="grpE"
                     /locus_tag="ACG8KN_001326"
     CDS             2958..3521
                     /gene="grpE"
                     /locus_tag="ACG8KN_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9188392.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3572..5401
                     /gene="dnaK"
                     /locus_tag="ACG8KN_001327"
     CDS             3572..5401
                     /gene="dnaK"
                     /locus_tag="ACG8KN_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9188393.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5552..6718
                     /gene="dnaJ"
                     /locus_tag="ACG8KN_001328"
     CDS             5552..6718
                     /gene="dnaJ"
                     /locus_tag="ACG8KN_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9188394.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7086..7823)
                     /locus_tag="ACG8KN_001329"
     CDS             complement(7086..7823)
                     /locus_tag="ACG8KN_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9188395.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7825..9327)
                     /locus_tag="ACG8KN_001330"
     CDS             complement(7825..9327)
                     /locus_tag="ACG8KN_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFP9188396.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIEKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9586..11580
                     /gene="uvrB"
                     /locus_tag="ACG8KN_001331"
     CDS             9586..11580
                     /gene="uvrB"
                     /locus_tag="ACG8KN_001331"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9188397.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKFLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11590..14406
                     /gene="uvrA"
                     /locus_tag="ACG8KN_001332"
     CDS             11590..14406
                     /gene="uvrA"
                     /locus_tag="ACG8KN_001332"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9188398.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14669..15934
                     /locus_tag="ACG8KN_001333"
     CDS             14669..15934
                     /locus_tag="ACG8KN_001333"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFP9188399.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15938..16669
                     /locus_tag="ACG8KN_001334"
     CDS             15938..16669
                     /locus_tag="ACG8KN_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFP9188400.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            16804..17688
                     /gene="rapZ"
                     /locus_tag="ACG8KN_001335"
     CDS             16804..17688
                     /gene="rapZ"
                     /locus_tag="ACG8KN_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9188401.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            17685..18683
                     /gene="yvcK"
                     /locus_tag="ACG8KN_001336"
     CDS             17685..18683
                     /gene="yvcK"
                     /locus_tag="ACG8KN_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9188402.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            18717..19652
                     /gene="whiA"
                     /locus_tag="ACG8KN_001337"
     CDS             18717..19652
                     /gene="whiA"
                     /locus_tag="ACG8KN_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFP9188403.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20195..20845
                     /locus_tag="ACG8KN_001338"
     CDS             20195..20845
                     /locus_tag="ACG8KN_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188404.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            21032..>21594
                     /locus_tag="ACG8KN_001339"
     CDS             21032..>21594
                     /locus_tag="ACG8KN_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFP9188405.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         7102 a         3658 c         4712 g         6122 t
ORIGIN      
        1 ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg taaaaaaatc
       61 atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg
      121 cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga
      181 aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta
      241 ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt
      301 tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac
      361 gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa
      421 tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt
      481 gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg cagccagtag
      541 atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt catcctacag
      601 cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct gctaaacaac
      661 ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta
      721 ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg aaaaactatg
      781 gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg aaaaaaattg
      841 gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa gcagaaaatt
      901 ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc
      961 gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga
     1021 tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg
     1081 atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac
     1141 gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca
     1201 tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt tatcttggag
     1261 atattcgtta ccgcaacaaa ggaccgatcc aacactattt acgcccatta cgagcaaatg
     1321 aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt
     1381 tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa
     1441 aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg
     1501 ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg aacaatgtgt
     1561 ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc gaaacttgaa
     1621 aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa ctggatttac
     1681 atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct
     1741 tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga atatatgagt
     1801 gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca
     1861 tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg tctgagggag
     1921 tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc
     1981 tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg
     2041 tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt
     2101 ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat
     2161 ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac cgccgactga
     2221 ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata
     2281 tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag
     2341 aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc
     2401 ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg acagaaggga
     2461 tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt tatgtcagtg
     2521 gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt
     2581 tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata
     2641 ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc
     2701 aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta
     2761 gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta
     2821 aacttgctga ttattaccgc tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct
     2881 atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat
     2941 gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg
     3001 ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg
     3061 aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagatagat
     3121 acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc
     3181 aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc
     3241 tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg
     3301 tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc
     3361 ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga
     3421 ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac aaattacatg
     3481 atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga tataaaactt
     3541 acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg
     3601 tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc
     3661 agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg
     3721 ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca
     3781 tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat
     3841 ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt
     3901 cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa
     3961 agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc
     4021 tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct
     4081 tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt
     4141 gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta
     4201 catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt
     4261 acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac
     4321 acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac
     4381 attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc
     4441 agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg aagttatttt
     4501 agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa
     4561 agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg
     4621 tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt
     4681 aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc
     4741 aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca
     4801 tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt
     4861 gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga
     4921 taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat
     4981 cacgatcaaa tcttcttcag gtctaacaga tgaagaaatc gaacgtatgg tgaaagatgc
     5041 agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt
     5101 agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga
     5161 agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg ttgaagcaaa
     5221 caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac
     5281 tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag aagcagctca
     5341 aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata
     5401 atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa
     5461 aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg
     5521 aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt
     5581 cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc
     5641 gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt
     5701 atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg accaatacgg
     5761 gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt
     5821 cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg
     5881 aggaggcggt cgatcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac
     5941 ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg
     6001 ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac aaccaactac
     6061 ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc cacttggacg
     6121 agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa tcaaagaacc
     6181 ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt
     6241 accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga
     6301 aaacggcgga ccatttggtg atttgtatgt ggtctttcgt gtagaagaaa gtgatatctt
     6361 tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt
     6421 gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg
     6481 aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg
     6541 tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca
     6601 acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca
     6661 agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt
     6721 tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta aaaacgctaa
     6781 gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctggtttt tttgtagttg
     6841 tcatgtttgt accaacaaaa aagccagctt tagaataaga taatcatcat agtttctgaa
     6901 ggtggtatcg actatgtact tgatatcgtc aatgaaccga tggtactttc ttatgcgatg
     6961 gaacaggtag agcgattgct tgtttgtgca ggagaacaag cgatgcggct ggtacagtta
     7021 agtgaaaaaa caagaaacta aaaatgcccg gtcgtcatga agcaaccggg catttttggt
     7081 ttttattaaa tattcaatac tttattcaag aaatcttttg tccgttcatt ttgcggattt
     7141 tcaaagattt gttgaggcgt tccgtcttct aaaaagtttc cgtcgtcaat gaacatgaca
     7201 cgattggcca cttctttagc gaagcccatt tcgtgcgtaa cgataaccat cgtcatacct
     7261 tgttctgcta actttttcat gacgttcaag acatcaccga ccatttcggg gtcgagtgca
     7321 gatgtaggct catcaaaaag catgatgtca gggttcatcg ctagagcacg agcaatagcc
     7381 acacgctgtt tttgtccacc agaaagcatt tcaggataca tatctttttt atcttctaga
     7441 ccaactgtct gcagtaattt gattgctttt ttctctgctt cttctctaga ttctttcttc
     7501 aggtccatcg gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa taggttaaag
     7561 tgctggaata ccattccgat atgttgtctc accaagttga tatctgtgct tttatccatc
     7621 aagtgggcgc cgtcaataat aatttcaccg tcagaaggtt cttctaaacg attcaagcaa
     7681 cgcaaaaatg tactttttcc tgaacctgaa gggccaatga tacagacgac atctccttcg
     7741 ttgatggata cattgatgtc gtttaatact gtgttatcgc cgtatttttt gactaaatgc
     7801 tcaactaaga ttttttctgc catcttactt caccttcttt tctaaaactt ttgctaattt
     7861 tgtcaatgct gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa catacgtact
     7921 ttgtgttgta cgtgcaacaa tgatcttacc agtttgcagc aattcgacta caccgattgc
     7981 ggaaatgatc gttgtgtctt ttaatgagat aacgaactga ttgacgaatg atgggatcat
     8041 gatgcgtatt gcttgtggca aaatgatttt ttgcattgtc cgattataag aaagacctaa
     8101 acttctggaa gcttccattt gtccgacagg aacggcgttg atcccgcctc gaacaatctc
     8161 tgcgatataa gcacttgcgt taagagtcaa ggtgataaca ccagcaagga agtcaggaat
     8221 cgtaaatcca acgataccag gcaacccaaa gaaaataaag aacgcaagaa ccatcattgg
     8281 aattcctcgg atgatatcga cataaatact agcaattgtg cgaagagcgc gtattggtgc
     8341 tacactgaat aatccaaaaa tcacaccgat tactatagcc aaagcaaagg agatcaaagt
     8401 taggagaatt gttctccaaa gtcctttcaa caatgcttga tagttgtttt ggataagtcc
     8461 aagaaatgtt gattcatcag cagtggaaga ctcactagaa gtttctgaac catccgctac
     8521 atattctttg ataatcttat cgtattcgcc tgttcttttt aattctttca atccttcatt
     8581 gaacatatta agaagctcag gattttgtcc ttttttaacg gcaaatgcat attttccgcc
     8641 agattctttt tcgatcggtg tttccaattc ttgtccttgt ttgatcgcat aaccgattac
     8701 cggataatca tccatcattg catcgattgc gccaactttt aacccgtcgt acaattgatc
     8761 agcagcatca aaatatttga tagaatagcc atattcagat tcatggtcgc ttaaaaattc
     8821 agcactttcg gtaccaactt ttgctccgac tgtttttcct ttcaaatctt cataagactt
     8881 gatcgaagta tctccttttt tgacagctaa ttggatcccg ctatcaaagt aagaatcaga
     8941 aaaatcatag cttttctttc gttcatctgt tacagtcatt ccggcaatca ttccgtctgc
     9001 ttgtcctgat tccaaagctt gcaaagcagc agagaaacca cggtagtcca ttcggatgtt
     9061 aaatccttgc agtttggcga tgttttccat caattttaca tcgatgcctt cgtactcgcc
     9121 ctttgcgttt tgaaattcaa atggagcaaa agcagagtca ctggcaatta cgtatacgtc
     9181 ttttttgggc tctatttttt gcattgtctc gcttgtgctt gactctgccg ttgtagtgtt
     9241 gtcagcttgt gcaacatgac caaacgcaag tgtcacaagt cctgtcatta agaaaacaaa
     9301 tgagtgaaat aaatgctttc ttttcataat caacctccta aatttccaat attttattat
     9361 tataggcgaa aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga aaaaaatgcg
     9421 agtttctaac attttatgtt ataaaacgta acatgtataa taagataaaa aagtggcgaa
     9481 agtatgttcg gtgtggtata attttttagt aaggaatgcc tttgctttcc ttttcttttt
     9541 attggtcgta aaatgaaaag gaatgaatga aaaggaggac gaagaatgat tgagagagat
     9601 acctcccgac actttgagct tgtctcgaaa taccaaccgg ctggtgatca gcctgaagcg
     9661 attaaccaat tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt attaggtgcg
     9721 actggaacag ggaaaacata tacgatttcc aatcttattg agaaagtgaa taaaccaaca
     9781 ctgattattg ctcataataa aacattggct ggtcagctgt acggggaatt caaggaattt
     9841 ttccctaaca atgctgttga atattttgtt tcttattatg attattatca gcctgaagct
     9901 tatgtgcctt caagcgatac gtatatcgaa aaagattcaa gtgtaaatga tgagatcgac
     9961 aaattacgac attcggctac tagttcatta ttagaaagaa atgatgtgat cgtcattgca
    10021 tccgtttcat gtatttttgg attaggttcg ccttttgagt accaaaaaca agtagtttcg
    10081 attcgacagg gagcagaact agatcgcaat cagttgattc gtgacttagt cagtatccaa
    10141 ttcgaaagaa atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg tgatgtcgtg
    10201 gagatttttc cggcttcaag agatgaacgt gccttacgtg tggagttttt tggagatgag
    10261 gtcgaacgta tccgagaagt caatgcactg acaggtgaag ttttaggaga aacggaacac
    10321 gtggcaatct tcccggcgac tcactttgtg acgaatgatg aacatatgga gcatgccgta
    10381 gcgaatatca aagcggaatt agaacagcgt ttaactgtct tgcgaaatga aaacaaactt
    10441 ctggaagctc aacgtttaga acagcgaacc aattacgata ttgaaatgat gttagaaatg
    10501 ggctacacgt cgggaattga aaattattct cggcatatgg acggacgtaa agaaggtgaa
    10561 ccgccttata cgctattgga tttctttcct gaagattttt tgatcgtggc ggacgaatcg
    10621 cacgtaacca tgcctcaaat cagaggaatg tataatggtg accgagcgag aaaacagatg
    10681 ctagtagatt acggtttccg tttgccatct gctttagaca atcggccact tcggttagaa
    10741 gagtttgaaa aacatgtcaa tcagatcatt tatgtctctg cgacgccagg accatatgaa
    10801 catgaacaaa cagataccgt tatccaacaa atcatccgtc cgacagggct gcttgaccca
    10861 gtgatcgaag ttcgtccaat catgggacaa atcgatgatc tagtaggcga aataaatgaa
    10921 cgagtcgaaa aagaccagcg ggtgtttgta acgacattga cgaaaaaaat ggcagaagac
    10981 ctcacagatt atttcaaaga attagggatc aaagtcaaat ttttgcatag tgatatcaaa
    11041 acgctggaac gaacagaaat cattcgagat cttcgattag gtgaatttga tgtgttagtt
    11101 ggaatcaatt tgcttcgtga aggattagac gtacctgaag tttcccttgt tgctatcttg
    11161 gatgcggata aagaaggctt tttacgtagt gaacgttcct tagtccaaac gattggacga
    11221 gctgctcgga atgaagaagg taaagtaatc atgtacgcag acaaagtaac agattctatg
    11281 cgtctagcga tggatgaaac gtcaagacgt cgtacgattc agcagaagta caatgaagaa
    11341 catgggatcg ttccaaaaac gatcattaaa gaaatccgag atttgatttc tattacgaaa
    11401 gaatcggaag atgatacaaa agaagcggta caagtctcgt acgaagaaat gacgaaggaa
    11461 gaaaaagaca cgttattgat gaaattagaa aaagaaatga aagatgcggc aaaagctttg
    11521 gattttgaaa cagccgcgaa tgtgcgagat atgattttag aattaaaagc ttcaaagtag
    11581 gaggacaaca tggctaacga taaaattacg attcatggtg cacgtgccca taacttgaaa
    11641 aatatagatg taacgattcc acgagataaa ttcgtggtcg ttacaggttt gtccggttct
    11701 ggaaaaagtt ccctagcatt tgatacgctg tacgctgaag ggcagcggag atacgtagag
    11761 agcctttctg cttatgctcg tcagttttta ggacaaatgg ataagccgga cgtagatagc
    11821 atcgacggat tgagtccggc gatttctatt gatcagaaaa caacaagtaa aaatcctcgg
    11881 tcgacagtag gtactgtgac ggaaatcaat gactatttac gcttgttgtt tgcacgtgta
    11941 ggacatccga tttgtcccaa cgatcatatc gagattacca gtcaatcagt agatcaaatg
    12001 gcagatcaag taatggcttt gcctgagcgc acaaagatcc aagtgttggc accgatcgtg
    12061 gtaaagaaaa aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga gggatacgtc
    12121 cgcgttcgag tcgatggtga gatctatgat ttaagtgaag tgcctgagct ggagaaaaac
    12181 aaaaaacatg atatcgcgat cgtcatcgac cggatcattg tgaaagaagg gattcgttct
    12241 cgcctatttg attctttaga ggcggccctg cgattagcag acggctatgc tctggttgac
    12301 gtgatcggtg aagaagaaat gttgtttagc gaacactatg cctgtcctta ttgcggattt
    12361 actgttggcg aactagaacc aagactgttc tcattcaatg cgccttttgg agcttgtcca
    12421 gactgtgatg gtttaggtgt caaattagaa gtcgatcaag atttagtgat tccagataga
    12481 acgaagacac ttagtcaagg tgctatcgtt ccatggaatc caatcagctc tcagtattat
    12541 cctcaaatgt tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac accattcgaa
    12601 gaattaccgg aagagcatca ggagatcatc ttgaatggga cagatgatct tttccatttc
    12661 cattatgaaa atgattttgg tggcgttcgc gatgtagaaa cgactttcga aggggtattg
    12721 aagaatatcg agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga gcaaatgcgt
    12781 ctttatatga cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa tccccaagct
    12841 ctatctgttc aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc tattcaaaaa
    12901 gcagtagatt tcttctcaaa cttaacgtta tctgaacaag agaaagtaat tgccaaaccg
    12961 attttaaaag aagtaaatga cagactttca ttcttggaaa atgtgggact agactatcta
    13021 actttgagtc gcgcttccgg aacattatct gggggagaag cgcagcgaat cagattggct
    13081 acacaaattg gatcgaatct ttctggagtt ttgtatattt tagatgaacc atcgatcgga
    13141 ttgcatcaac gcgacaacga tcgactgatt ggctcattaa agaaaatgcg tgatcttggc
    13201 aacaccctcg ttgtagtcga acatgatgaa gatacgatgt gggcagcaga ttatttgatt
    13261 gacgtcggac ctggggccgg agaacaaggc ggacaaatcg tggctgcagg cactcctgaa
    13321 gaagtagcta atgatgaaaa ttctttgaca gggaaatatc tttcagggaa aaaatcaatc
    13381 cctgtaccaa aagaacgccg taaaggtaat gggaaagcaa tcaagatcac tggtgcttca
    13441 gaaaacaatc tgaaaaatat tagtgttgaa tttccattag gagaatttgt agcagtcaca
    13501 ggtgtgtctg gttctggaaa gagtacattg gtcaatagta tcttaaagaa atcgttagcg
    13561 caaaaattaa ataagaattc tgctaagcca ggtaaattca agacaatttc cggctacgaa
    13621 agtatcgaaa agatcatcga tatcgatcaa agcccaatcg gccggacgcc gagaagtaat
    13681 ccagcgactt atacaagtgt atttgatgat atccgtgggt tatttgctca aacgaacgag
    13741 gcaaaaatgc ggggttataa gaaagggcgt tttagtttca acgtaaaagg cggtcgttgt
    13801 gaagcttgtc gcggggatgg aattattaag atcgaaatgc actttttgcc tgatgtctat
    13861 gttccttgtg aagtatgtca tggtaaacga tataattctg aaacattaga agtgcattac
    13921 aaaggaaaaa gcattgctga tattttggaa atgacagtag aggatgctgt agaattcttc
    13981 aagcacattc caaagattca tcgcaaactg caaacgattg ttgatgttgg cttaggttat
    14041 gtgacgatgg ggcaaccagc aacgacattg tccggtggtg aggcacaacg gatgaaactt
    14101 gccagtgaat tgcacaaaat ctctaatgga aagaatttct atatactaga tgaaccaacg
    14161 acaggacttc atagcgatga catcgcccgc ttgttgcatg tattacaaag attagtagat
    14221 gctggtaaca cagttttagt gattgaacac aatctagatg taatcaaaac agcagattat
    14281 atcattgatt taggaccaga aggtggagaa ggtggaggaa cgatccttgc gactggaaca
    14341 ccagaagaaa tcattaacgt aaaagaaagt tatacaggtc actatttgaa aaaaataatg
    14401 gtataaagag gatagatagc tgagatagtt aaaaaaacat aaaaaaatga atttttgctt
    14461 gttttattaa tatcagtgtt ctagaatcat tacatggtag ttctttctac actgattggt
    14521 atagataaaa acttcctata agttcaatcc gttcaaaaaa gaattgagca atcggaggag
    14581 tttcctttga ttgctttttt ctttatggta tgatattcct tgtgtagttg gaaaagaaaa
    14641 acaagagatt atgagtaagt gagagataat gatgaacagt attgaaaatg aagaatttat
    14701 ccctatatta ttaggaagcg acatgaacgt ttatggtatg gcacgttctt ttaatgaagc
    14761 atacggcaaa atctgccaag catatgcttc ggatcagtta gcccctaccc gctacagtaa
    14821 aattgtcaat gtagaagtta ttccaggatt tgataaagac cctgtattta tcgagacgat
    14881 gcttcgtttg gcaaaagaaa gatattctga taagagtaaa aaatatcttt tgattgcttg
    14941 tggtgatgga tatgcagaat tgatctccca acataaacaa gagctgtcag aatattttat
    15001 ctgtccatat attgattatt ctttatttga aaggctgatc aataaagtaa gcttttatga
    15061 agtttgtgaa gaatatgatc ttccgtatcc taaaacattg atcgtcagag aagaaatgtt
    15121 agtaaatgga catttggaac aggaattgcc ttttgaattc ccagttgctt tgaaacctgc
    15181 aaacagtgtg gagtatcttt ctgtccaatt tgaaggaaga aaaaaagctt ttattttaga
    15241 gactagagaa gagttcgatt taattttagg ccggatatac gaagccggtt ataaaagtga
    15301 aatgattgta caagatttta tcccgggtga tgatagtaat atgcgtgtgc taaatgcgta
    15361 tgtagatgaa gatcatcaag ttcggatgat gtgccttggt catccactat tagaagatcc
    15421 tacaccagca tcgattggta attacgttgt gatcatgcca gattataacg aaaaaatcta
    15481 tcaaaccatt aaggcatttt tggaaaaaat cgaatataca ggctttgcca atttcgatat
    15541 gaagtatgat cctagagatg gagaatataa attatttgaa atcaatttga gacaaggtcg
    15601 tagcagtttc tttgtgacat tgaacggctt gaatctggct cgttttgtca cagaagatcg
    15661 agtgttcaac aaaccctttg ttgaaacgac ctatggaacg aatcaatcag ataaagcaag
    15721 attatggatg ggtgtgccca aaaaaatctt tttagaatat gctcgggaaa atgaagacaa
    15781 aaaactagct gagcaaatga tcaaagaaaa tcgttacggt actactgtat tttatgaaaa
    15841 agaccgatca atcaagcgct ggttgttgat gaaatatatg ttccataact atattccaag
    15901 attcaaaaag tattttcatg tgaaagaagg ctagttcatg gagaattttt tcagtatttt
    15961 aggcggaatg ggcacgatgg cgacagaaag ttttgttcgc ttgatcaacc accgaacgaa
    16021 agctacaaaa gatcaagaat atttgaatta tgtcttattc aatcatgcaa cagttcctga
    16081 tcgtacagct tatattttag atcgatcaga agaaaatcca atgccgtttc tgctggatga
    16141 tattgagaaa caaaatttat tgcgaccaaa cttcattgtg ttgacttgta acaccgcaca
    16201 ctactttttt gaagagttac aagcggcaac ggatattcct atcttgcata tgccaagaga
    16261 agcagcaaat gaacttgtcc gtcagcatac gacagggaga gtagcgattc taggaacaga
    16321 aggaagtatg aaagctggaa tctatgaacg cgaagtcaaa aatcttggtt tcgaaacagt
    16381 gattcctgat actgcgcttc aagaaaaaat caattacctg atctatcatg agatcaagga
    16441 atcggattat ttgaatcaag agctctatta cgaaatctta gaggaagcgg tagagcgcct
    16501 aaactgtgaa aaagtcatcc taggttgcac agaattatct ttgatgcatg aatttgctga
    16561 agataatcat tacccagtga tcgatgctca atctatttta gcggatcgaa cgatcgaacg
    16621 tgcattagct gaaagaagcg aggcattgga tacagcatcg gaaaagtaaa aaaattggat
    16681 ttggggcaaa tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga aaaagaaaac
    16741 tggtaatgat acgcccggga tgatataatt ttattatgca caatgtgaga ggggttcgta
    16801 aatatgacag ataatttgca attagtcatc ataacaggta tgagtggtgc agggaaaaca
    16861 gtagctatcc aaagttttga agatatgggc tatttttgta tcgataatat gccgcctcga
    16921 ttgattccaa aattctggga attgatcaaa gaatcaggta aagtcacaaa aatggcttta
    16981 ggagtggatt tgcgttctcg ttccttcttt gaagaaatcc aaaacatgct gatcgaaatc
    17041 gaaaatacga acttcatcga cacttctatc ttatttttag atgcttcaga cgaagaacta
    17101 gtggctcgct acaaagaaac aagacgagca catccaatgg cgatggacgg tttgatcact
    17161 gaagggatac gtaaagaacg ggcgatacta gacgacttga aagcccaagc ttccgtgatc
    17221 attgatacga caacattgac accacgacaa ttacgtgaaa aaatcaatga gtcattcaaa
    17281 tcaaagggag accaaggatt tcggatcgaa tttgtatcat ttggttttaa atacgggttg
    17341 ccgatcgatt cggatattgt gatggatgtg cgttttttgc ccaaccctca ttatatcccg
    17401 gaactaagac cattgaccgg aaaagatcag ccagtctatg attatgtgat gtcctttgat
    17461 gaaacgcaat cattctacca taaatttttg gatcttctgg aaactgtatt gcctggatac
    17521 attaaagaag gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg tcaacaccgt
    17581 tctgtggcat tgactgaacg tttggcaaat gctttaggga aaaactataa agtcaatgtg
    17641 acacatcggg acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa atgaaaacct
    17701 accggataag aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg ccagttatcc
    17761 tgaaaagttt acgaaatcaa ggagcagata tcacagcagt agtgactgtt gcagatgatg
    17821 gtggaagcag cggtgcaatc agagaatcga tcgctatggc tcctcctggt gacttgagaa
    17881 atgtcttagt ggcgttatcg gatatgccac aattctatga agatattttc caataccgtt
    17941 tcaaaaagga agatcagttt ttagccaatc atacgatcgg taatctgatc attgccgcag
    18001 tttccgagat gcggggaagc acttatgagg cgattcaatt attagcaaaa atgatgcatg
    18061 tagatggaca tgtttatcct tcttcagaaa ctcctttgac cttgcatgct gtttttaaag
    18121 acggaacgac tgcagtagga gaatctaaaa tcgcgatcaa ccgtaaaacc attgaccgtg
    18181 tgtatgtctg caacacaaat gataaaagtg aagccaaagc ggctcgaaaa gtcgtttcag
    18241 caattatgga cgccgacatg gtcgttttgg gacccggaag tttattcacc agtatccttc
    18301 caaatttagt gataccagaa atcggcgaag caatgctcaa gacaactgct gaaaccgtct
    18361 atatctgtaa tatcatgacc caaaaagggg aaacggaaca tttcacagat gccgatcata
    18421 tacgtgtttt acacaagcat ttgaatcagc cttttatcga tacagtattg gtcaatacgg
    18481 aaaaagtgcc tgaagattat atggacccag agatctatga tgagtatcta gtacaagtcg
    18541 cacacgactt caacggattg agaaatgaag gatgtcgagt gatttccacc gactttttag
    18601 aattgaaaga tggtggtgtc ttccacgatg gagacaaagt agtagaagaa ttgttccgtt
    18661 tagttttcgg ttcaaaatat taagcaattt attgttggag aaaggaggag aataggatgt
    18721 catttgctgc agatgtgaag aaagaactaa ctggtttgga agttcatcga gaacatgcga
    18781 aagcggaatt agctgctttg ctgcgaatga acggctcttt aggtattgtg aaccagcagt
    18841 ttattttgaa cgtccaaaca gaaaatgctg caattgccag acgtatctat tctcttttga
    18901 aagatcatta tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa ttaaaaaaga
    18961 acaatgtcta tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg gatcttgata
    19021 tcatggatgg tatgatgttc aatggacacg tttcggacga gattatggga aattcgcaaa
    19081 aaatgcgttc ttacttacgt ggtgctttta tggcctcagg ttcagtcaat aatccggaaa
    19141 cgagtcgtta tcatttggaa atattttcta tttatgaaca gcataaccaa gacatttgcg
    19201 atatgttaaa ttattacggt ctacatgctc gaacgcttga acggcgcaat ggctatattt
    19261 cgtatttaaa aggagcagaa aaaattgctg atttcttaac attgattggt gcaaccaact
    19321 caatgctccg atttgaagat gttcgaatcg tacgggatat gagaaattcg gtcaatcgtc
    19381 tagtcaattg tgagacagca aatctgaaca agacgattga tgcggcttct aaacaaatcg
    19441 aaaacatcga attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa aaacttcagg
    19501 aaatcgcaga acttcggctt caacatccag aggtcagctt gaaagaatta ggggaaatga
    19561 ttccctcagg tgcaatatca aaatcgggaa ttaatcaccg aattcgcaaa ataaatgaat
    19621 ttgcggaaaa actgcgtgaa gaagcagttt aatccaaaga aaaaactgat acatgaagca
    19681 ggagatgcga atgtatcagt tttttttaaa agctatactt gcttttcttc cgtcaaacta
    19741 taattccaac tttactggag taacataatg ttgaaaggct tctattgttt tttaatacaa
    19801 tcgtataaaa agcggggaga caactgaatg aagattttag ttttatacat aaacttttgc
    19861 ttaatataga agtcctgata tagataggat caagtattta tctataactg gagattaatt
    19921 aaaaactgga aggaactgct cgatcaacct tgagaactgt ttttcttact tctggtcaag
    19981 attctcgtgg ttggtggata aggatggttg ataagttcat tggattagct agtcgaaaac
    20041 aaccaggtgt aattaatgta gaagttttaa caaggggatg gagtgattta ttcagtgata
    20101 atcataagtt cataaatagt aattgggatg tatggttggt atacgttata cctgaacttg
    20161 aactatcaag atttggttta gaatttgaga atcgatgttt ataaagggag ggcaaaaaat
    20221 gtgctttttt acaactttta ctatcattat gattgcagta ctttttattt ttctagatat
    20281 agcaaagcga aatacagcat ttttattata tcgggtttta cttcgagcag gtctcataac
    20341 atttatatcg atcgttggat tttttctatt cacagttatt gtatttattt ggagaactac
    20401 agcaccacca ttgccagaga ttacatatgg agaatttccg tttagtcttg aatatgaaat
    20461 agatgaggaa agaattttga ttgaagatac ggtgattgca gaatttatag gaagttttag
    20521 agggaatgtg acaactcaac ctagacgaag ttggagtata aaacttttaa ataatagtgg
    20581 agagttgaaa gcatttcatt atgaattctt aataatagaa agtgaagatg taacaatcac
    20641 tttaagtagt ggctctgctg gatattttat gaatgatggt ggaagcaagc cacagctcac
    20701 agtccgtgat cgtattgcag gttttatgca tttttctcac tatgacttag aagaagctcg
    20761 taaaatactt gcagaatatg ggatcacatt aatcaattgg gaatatgcgc caccgataag
    20821 aaattttttc aatgattatt attagtctta taaaaaacag aatgtaaaat ttatgtgcgc
    20881 gagtatttag ttttccattt tttgtttgat agatatatag cattgttgaa gcatgggata
    20941 gtggtgccaa ttttatcaat gaggttggtc aggcagttaa cataattggt gatgctataa
    21001 atccatttaa ttggaattgg aggaattagg tgtggaaaca gtgagattat ggatattcat
    21061 gaaaaatttt cgttcggctt tgagatatgt tattctggaa tcaaaatcag gttattatct
    21121 gattgacaca gatcactata ctttttgggg aggctacctt tttccgattt ttaattggat
    21181 aatcccccat aaggttatga aaattaactt gcctgcagaa gaaataaata gattaatact
    21241 caacaaagat ttacaaagga aaagagagaa agaacattct aatgttgctt ggataggtcc
    21301 agcatttgca gtagtgactt cttcaattgt cggtcctatg ctagagagta tttatagtga
    21361 tttttctatt ggaatacgtt atttatttgt tactttaagt atagctgttc ttttgtgcat
    21421 aaagggttat gttagcaaga agaataaaga tattttaaca ctaatcggtg taaacaattt
    21481 atctagtact aaaattatac tgtttcctga atctataaaa gaaggatcaa ggttgttgat
    21541 aatttttata atgcttatac tgttttctgt tttatttggg gtgatgttta taga
//
LOCUS       DAZKCZ010000038        21001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000038 DAZKCZ010000000
VERSION     DAZKCZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.038"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            682..1371
                     /locus_tag="ACG8KN_001340"
     CDS             682..1371
                     /locus_tag="ACG8KN_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9188406.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1358..2566
                     /locus_tag="ACG8KN_001341"
     CDS             1358..2566
                     /locus_tag="ACG8KN_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9188407.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2860..4131
                     /gene="serS"
                     /locus_tag="ACG8KN_001342"
     CDS             2860..4131
                     /gene="serS"
                     /locus_tag="ACG8KN_001342"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9188408.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4680..6164)
                     /gene="guaB"
                     /locus_tag="ACG8KN_001343"
     CDS             complement(4680..6164)
                     /gene="guaB"
                     /locus_tag="ACG8KN_001343"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9188409.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6669..7364)
                     /locus_tag="ACG8KN_001344"
     CDS             complement(6669..7364)
                     /locus_tag="ACG8KN_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFP9188410.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7454..8554)
                     /gene="ychF"
                     /locus_tag="ACG8KN_001345"
     CDS             complement(7454..8554)
                     /gene="ychF"
                     /locus_tag="ACG8KN_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9188411.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8563..8754)
                     /locus_tag="ACG8KN_001346"
     CDS             complement(8563..8754)
                     /locus_tag="ACG8KN_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFP9188412.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8775..9665)
                     /locus_tag="ACG8KN_001347"
     CDS             complement(8775..9665)
                     /locus_tag="ACG8KN_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9188413.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9652..10419)
                     /locus_tag="ACG8KN_001348"
     CDS             complement(9652..10419)
                     /locus_tag="ACG8KN_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9188414.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10431..11144)
                     /gene="rsmG"
                     /locus_tag="ACG8KN_001349"
     CDS             complement(10431..11144)
                     /gene="rsmG"
                     /locus_tag="ACG8KN_001349"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9188415.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11376..13277)
                     /gene="mnmG"
                     /locus_tag="ACG8KN_001350"
     CDS             complement(11376..13277)
                     /gene="mnmG"
                     /locus_tag="ACG8KN_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9188416.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13294..14691)
                     /gene="mnmE"
                     /locus_tag="ACG8KN_001351"
     CDS             complement(13294..14691)
                     /gene="mnmE"
                     /locus_tag="ACG8KN_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9188417.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14990..15373
                     /locus_tag="ACG8KN_001352"
     CDS             14990..15373
                     /locus_tag="ACG8KN_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188418.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15541..16299)
                     /gene="jag"
                     /locus_tag="ACG8KN_001353"
     CDS             complement(15541..16299)
                     /gene="jag"
                     /locus_tag="ACG8KN_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFP9188419.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16317..17135)
                     /locus_tag="ACG8KN_001354"
     CDS             complement(16317..17135)
                     /locus_tag="ACG8KN_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFP9188420.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17132..17488)
                     /gene="rnpA"
                     /locus_tag="ACG8KN_001355"
     CDS             complement(17132..17488)
                     /gene="rnpA"
                     /locus_tag="ACG8KN_001355"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9188421.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17606..17740)
                     /gene="rpmH"
                     /locus_tag="ACG8KN_001356"
     CDS             complement(17606..17740)
                     /gene="rpmH"
                     /locus_tag="ACG8KN_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9188422.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18263..19597
                     /gene="dnaA"
                     /locus_tag="ACG8KN_001357"
     CDS             18263..19597
                     /gene="dnaA"
                     /locus_tag="ACG8KN_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9188423.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19795..20925
                     /gene="dnaN"
                     /locus_tag="ACG8KN_001358"
     CDS             19795..20925
                     /gene="dnaN"
                     /locus_tag="ACG8KN_001358"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9188424.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         6006 a         4273 c         3847 g         6875 t
ORIGIN      
        1 ttaaaaatgt ttgttgtctt gaattgaatc aagacgccct acacatttgg tttgtcttac
       61 tttgttcagt tttcaaaggt ctacttcatc aggagtgttt cccgattcgt ttttggctgc
      121 cgtagcaact tttatatcat atcagagaga caagtaattg tcaactcttt tttaaaagtt
      181 ttttcgattt cgtttttcga aatcttccag caactcattt atcataactt atcgtttgtt
      241 atttgtcaag aacttttttg aagttttttt cgatagaatc aagcttcgca agtggttctt
      301 ttttaacgac tcagttatct tatcatgtca tttccaacat gtcaacaact tattttttat
      361 tatttaataa gttattggtg ttcttttgaa caaataataa tatatcaaca tcacatgtga
      421 aagtcaataa gaaaatgctt atttatcaac atttctagct tacatgatca ttggttagtt
      481 ctggtccttt ttcgttcgtc tttataactc cgagagacgt aaaatccttt tgttttcagt
      541 ttaatgttca tgttcttgtc ttattcacac caactagatg tctcaccttc taaaattttc
      601 cttaacaaag tgctgctttt atctatttct gctttttttt gctagaataa tagtaacaat
      661 atgactctgg aggatttatt tatgaaaatt ttgattgctg acgatgataa agaaatcgtt
      721 gaattattaa gtatctatgt tcacaatgag gggtatgaag cagtaaaggc ttatgatggg
      781 aaagaagcat tatctaaaat cagaacaatc cctgatattg aattacttat tttagatatc
      841 atgatgcctg agatggatgg gatgcaagta gtgaaagagc tacgaaaaga atcccaaata
      901 ccaatcatta tgctgacagc aaaaactact gatatggata agatcaaagg actggtcgct
      961 ggtgcagatg attatgtgac caagccattt aatccgttag aagtaatggc tagagtaaag
     1021 tctttattac ggcgtacaaa aatgcagatt gccccagatc agcctgatgt attagaagta
     1081 aatgcattaa tcatcaataa agactctcat gaagttaaaa caatcgatgg gaaagaaatc
     1141 cagttaacag ctttggaatt cggtatcctc tacttgcttg ctagtcatcc taatcgagta
     1201 tttagcgcgg atgaaatttt tgagcgtgtt tggaaacaag aaagtattgt ctctgctaaa
     1261 actgtcatgg ttcacgttag tcatttaaga gataagattg aagaagcaac tggtggtgaa
     1321 aaagtcattc aaaccgtttg gggagtgggc tataaaattg acgcaagata gtaaacgagc
     1381 aactgaaaaa cgccgtcgga tcaatttgac ttcaaaagaa atcagcgaat tgttagcgga
     1441 aggaatcatc acgattatcc ttttgcttct attaaatgtt tctattctgg ttgttatcag
     1501 ttcggtgatc aacagttcac cttctttgac gaatgcgatt tgggattcaa agaatatttt
     1561 tgcagaacga ttgaacactg atttgttctg gaacggccgg aattttatta ttcccttttt
     1621 ctttctatta gatattggtg ttttgtattg gcgcttgatt cgaagatatc gccaaatgca
     1681 attgcggcat atcatcagcg aacttcacta catcgcaaac ggaaattatg atcatcgtat
     1741 cccttttgaa ctgagtggtg acttaagccg agtggtaaca agtatcaatg gattggtgga
     1801 tagtaccgta gcagcgattg aagacgaacg taaaatcgaa aaatcaaaag atgaattgat
     1861 cacgaatgtc agtcatgata tccgtacccc actcacgtcc atcattggat acctaggact
     1921 gatagaagat ggccagtatc actcagaaga agatctttta aagtatacgc atactgctta
     1981 tatcaaagca aagcagatga aatctttagt ggacgactta tttgaataca ctaaggtacg
     2041 ccagcctgct gtcccggtga atttttctgc ctttgacatg atccagttga tcgaacagtt
     2101 agcagctgat tttgagttgg aagcttctaa aaaaaatatc caaatacttg ttcaatcaaa
     2161 agtagactcc cttatcatgg atggcgatac cgaaaaactt gtacgtgtct tcaataatct
     2221 tttgacgaat gcgttgaaat acggaaaagg agcaacgaag atcgtaatcg aagtagaacg
     2281 tatcggttca gaagttgttg caacagtcaa aaataacgga gcaatgatcc cacaacaggc
     2341 aatcgataat ctattcgacc gtttttatcg tgtagaagaa tctcgttcac aagcaactgg
     2401 aggaactgga cttggtttag caatcgctca aagcattgtg gctttgcacg gcggatacat
     2461 ttatgcaaaa tcagataagc aatggacttc tttcatcatg caccttccaa tcaagaaaaa
     2521 tgaaaaatta cctattgata cacaggaagt tattgacgaa agctgaaata ttcgctattc
     2581 ttattacaat tattagaata aatgaaaaac acagaaccca gtacacttta ttctttttca
     2641 gagagctgat ggcaggtgta aatcagcaaa ggataatttg gaagttccag ttcgtttacg
     2701 tgccctttcg aaataagaaa ggtcggttca acagccgtta tgtgttcaac aagtgcagct
     2761 gacgctgaac ttgggtggta ccgcgaattt cagactgatt cgtcccaaga ataatgggat
     2821 ggatcagtct ttttttattt tcaaaaagga ggaaatcata tgttagatgt aaaaatgatg
     2881 cgacaaaata ttgaagaagt acaagaaaaa ttgatgactc ggggcgtaaa aaaagaaaca
     2941 ttagctgaat tcatggaact agatgaaaat cgtcgtaagc tactagtcaa aagtgaagaa
     3001 ttaaaaaaat atcgaaatga tgtttctgct gagatcgccc aattgaagcg tgcaaaagaa
     3061 gatgcttctg ctaaaatcgc tgaaatgaaa gaagtcggtg gcaacatcaa agcattggat
     3121 actgaaattg ctgaagtgga tgaaaaattg aaacaaattg caactacttt gccaaacctt
     3181 ccccatgatt ctgtgccagt tggaacagat gaagaggaca atgtagaaat ccgtcgttgg
     3241 agcgaaccac gtacatttgc ttttgaacca aaaccacact gggaaattgc agaaaatctt
     3301 gatattttag attttgaacg tggcgcaaaa gtagccggta gccgttttgt ctattacaaa
     3361 ggattaggtg ctcgattaga acgtgctgtc tacaacttca tgcttgatca gcatatttac
     3421 gaacacggct atactgaaat gatcactcct tatatcgtca acagtaaagc gatgtttggt
     3481 acgggacaat ttccaaaatt caaagaagat gttttccaat tggagaatac tgatctaaca
     3541 ttaatcccaa cagctgaagt accattaact aactattata atgatgaaat tttagatggt
     3601 gcagatttac ctatttattt cactgcacta agccctgctt ttcgttctga agccggaagt
     3661 gctggtcggg atacacgagg actgattcgt cttcaccaat tcaataaagt agaaatggtc
     3721 aaattttcgg atgtagagca ttcttacgaa gaattggaaa aaatgacagc tgatgcagaa
     3781 gatatcttga aaaaattgca tttgccttat cgtgtgatgg ccttatcaac tggagacatg
     3841 ggcttttctg cagctaaaac ttatgactta gaagtatgga tacctgcaca agatacttat
     3901 cgtgaaatca gttcttgttc taactgtgaa gatttccaag cacgccgcgc aatgatccgt
     3961 tatcgcgata atgatggaaa aatccactat actcatactc tgaatggttc aggacttgca
     4021 gttggccgta cagtagctgc cattttggaa aactaccaaa atgaagatgg ttctgtcaca
     4081 gttccagaag cgctcgttcc ttatatgggg aatctgaaag ttattaaata aagaggctgt
     4141 gaaaaagccg cttagtttcg agtattaaga aaaattttgt gaaaatggct ttccacattt
     4201 ttgcataaat tggacttaat accgagaaac tggcttttga acaccattta tcgagaggtt
     4261 gtgaaatcag cgaattaacc tgagtattag agaaaaaaat ggaaaaatag tccctcatat
     4321 tttaccgttt gttgaactaa taccgagggt cagctgattg aacaccgttt agtggagagg
     4381 tcgtaagcct accccccagc tcctagttac aaggacaaat tcaaaaaaca gctcttcata
     4441 ttttatgaat gttgtacttg tgattcggag gtggcaaggg aacactgttt attcaaaaag
     4501 aggctgggac agaagcgttt aactccaaga aataagaaga aattcacgaa aattgctttt
     4561 caaatttttg tgaatttctt cttatttccg aaggagttgc ttctacttcc gccgtttata
     4621 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct ttaatgtttt
     4681 taagattcaa ctgaatagtt tggtgcttct tttgtgattt gtacatcatg agggtgtgat
     4741 tcttttaatc cactgccgct catttggata aattgcgctt catcacgtaa ttgctgtaga
     4801 ttggctgccc caacataacc cattcctgaa cgcaaaccgc cgatcatttg gaaaataata
     4861 tctgcaacac ttcctttata agcaacccga ccttcgattc cttctggtac taatttattt
     4921 gcttcattga cactaccttg gaagtaacgg tcttttgaac ctttttccat cgcgcctaat
     4981 gagcccatac ctcggtatgt tttgaatcga cgtccttgga agatttcgaa ttcacctggt
     5041 gattcatctg tccctgccaa catacttcct agcatcactg catgtccgcc ggcagctaat
     5101 gctttgacga tgtcaccaga atatttgatc cctccgtctg caataatcgc tttgccatat
     5161 tgacgagcaa ctgatgctgc atcataaata gctgtgagct gaggtacacc tacaccggct
     5221 accacacgtg tcgtacaaat cgatcctggt ccgataccaa cttttacgac atctacacct
     5281 gcatcatata aagcttttgt cgcttcagcc gtcgcaacgt ttcctgcaat caatgttgca
     5341 tctgcaaaag ttgaacggat ttcttgaatt tttcggatca cacctgcact atgtccatga
     5401 gctgtatcga tgatgattgc atctgcccct gcttctaata aagcattcgc acgctcaaat
     5461 gtatcgcttg ttacaccaac ggctgctgct acaagcaatc gaccatgttc atcttttgct
     5521 gcatttggaa attcgatcac tttctcgatg tctttgatcg tgatcaatcc gcttaaacga
     5581 ccttcgttat ctacgattgg taatttttcg attttatgtt tttgcaaaat cttttctgca
     5641 tcttttagag aggtacctac tggggcagtc actaagttgt cttttgtcat cacatcagca
     5701 atcggcatgt gataatctgt tacaaaacgc atgtcgcggt ttgtaattat ccctaccaat
     5761 tttctgtttt caagtgtttc aacgattggc acaccactga tacgatattt gctcatcaat
     5821 tcttctgcgt ctgctacaag atgctgtggc gttaagaaga acggatcaat gattacgcca
     5881 ctttcagaac gtttgacttt tcttacttca tccgcttgct ggctgatcgt catgttttta
     5941 tggatcacac ctagtccgcc ttgtcttgcc atagcaatgg ccatcttgct gtcggtaaca
     6001 gtatccatac ttgcactgat gattggaata ttcaacttaa tatttttagc taattgtacg
     6061 ctcatatcca cttcgtttgg taacacgtga ctttctgctg gtatcaataa gacatcatca
     6121 aaagtaaatc cttttttcgt gaactttgtt tcccagttgg acatttttag taccactcct
     6181 ctaatttagt ttatctatta aaataattga ttttttcatt taagtcaatg atctacttgg
     6241 aatcaagtta gctttctcat agaataccaa tataaagcgt ttacatctgt gtatattaaa
     6301 agagattgta aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt
     6361 ctcatgtttt tgaattggtt gggtttatta ccgcagatgc aaacttttga acaccgttta
     6421 gtggagaggc tgtgaaaaag ctgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
     6481 gctttccaca tttttgcata aattggactt aataccgaga aactggcttt tgaacaccgt
     6541 ttatcgtaaa aagggactgt gacaagactt ttgtcacagt cccttactat tatttaacta
     6601 tggataaaca aaatgaggtt ctttactacc cctagatatc tagcaaaata gctgatcttt
     6661 ttgctagttt atcttcttgg gacggataaa cttccaataa tcccgtattt tttcttcaag
     6721 tagtaacgga ttgctaaagc agttgccccg ataatgataa ctactactgg atctaatact
     6781 ggattgataa ctggtggcaa tagtgttgtc gcagaaaaca gtacgaccca aagaagtgtc
     6841 gttccaccta ggataagcaa tgtcttaccc attcctggtt tcttgcttcg atctgctccg
     6901 ggatattcat attgatagat gtatttgtac atcatataaa agacccagcc accgattgca
     6961 gaacctagaa cgagtgtaga aaagccataa gctggcgttg ttccacgtga aaacaatccg
     7021 gtaatcgcaa tcatgatact taatagacca agcaacagca acgtattatc taaccacatc
     7081 aataaagggg tggatgaatt ttttacttct gctggcttat tgatgattgc ttccgttctt
     7141 tcagagactg tcccaaacaa ttgacgggct gttcttccgc ctttttgttc tgcaactaaa
     7201 gtaggcagaa tgtcatgaag ggcttttact ttttcttctt cagacaaatt cgctgcttct
     7261 aaagattttt tcaaatcaaa aatgtactgt tgatttcttt tggttaattt tgtttctaaa
     7321 ctattgtttt cttttacgat tgctcgtaat tgttctggtt ccattatcta atagtcctcc
     7381 ttagccgttc cttttctcaa cggtcgtcac aaaagacatt ctaccatatt ctacagctag
     7441 tttgatagcc cttttacaca ttaaagcgga ataacattac gtcgccgtct tgtacgacat
     7501 aatcttttcc ttctaatcga acacgtccag cttcttttgc tgcatgcatg tttccgtaat
     7561 gatccaagtc ttcaaaagag acagtttctg cacggataaa tccgcgttcg aaatctgaat
     7621 ggatgattcc tgctgcttgt ggtgctttga ttcctttttt gaatgtccaa gcacgtactt
     7681 cttgttcccc agcagtaaag taagtagcta agcctaacaa atcataagca gcacggatca
     7741 actgatccaa acctgattct tcgattccta aagcttctaa aaattccgct ttgtcttctt
     7801 catctaattc tgcgatttct tcttctgcac gagcactgac cacgatcact tctgcatttt
     7861 cttctgcagc aaagttgcgc acttcttgta cataatgatt cgcgtctgca tctgctacgt
     7921 catcttctga gacattggct acataaagaa ttggttttgt cgtcaataaa aatagtgatt
     7981 taacgatttt ttgttcttct tcagtaaatt cgatcgttcg ggcagatttt ccttcttcca
     8041 gtacaggttt gattttatcc agtacagcta gttctgccac tgcatcttta tctttggttt
     8101 tagcaacttt tgctacacga gtatagcgtt tgttgataga ttctaagtct gctaacacta
     8161 gttccaagtt gatcgtatca atatcttcta gtggatcaac acgtccttct acgtgagtaa
     8221 tattatcatc atcaaaacag cgaacaacgt gacaaatagc gtctacttgg cggatatggc
     8281 ttaagaattg atttcccaaa ccttctcctt tacttgcgcc ttttacgatt ccagcaatat
     8341 ctgtaaattc aaaagtcgtc ggaacagttt ttttcggatg tacaagttct gttaagcgtt
     8401 gcaaacgcca gtcaggtact tctaccatcc ctacgttggg gtcgatcgtc gcaaagggat
     8461 agtttgctgc ttctgctcct gcttttgtga tcgcattaaa caaagttgat tttcctacgt
     8521 tgggcaaccc aacaattcca gcagtcaatg ccataaattt attcactctt tctctgcatt
     8581 tttttcaatt acttttttta atttcttttc aaaatctcga cgggtcatca tgacgatatg
     8641 tccgcaattc atacatctga ttttgatatc tgcgcccatt cgaatgactt cccagcgatt
     8701 cgtctggcaa gcgtgaggtt ttttcatttc aacgatatcg tgcaagtcgt acataaaaat
     8761 agcctccttt ttgtttattc ttcatcaaat tgaatattca agatatcaag aattcgagtc
     8821 aaatcttttt gagaaagata ttcaatctcg atcttacctt tcccattttt ttcttggata
     8881 gctacacttg taccaaattt atccatcaaa cgatcttcgc tttctcggat ataatatggt
     8941 ttttcctttg ctagccttgg gatttttttc ttttcttttc catcattttg atttaactca
     9001 gaaacaagct gttccagttg gcggacagtt aaattttcag ttactgcacg attggctaat
     9061 ttcaagatca atgttttatt ttttaaacct aacagcgtac gggcttgtcc catagataaa
     9121 cgctgatctt gtaccatttc cttgacaaga tccggtaaag aaagcaagcg caaataattg
     9181 gcgatataag gtctgctctt tcctaaacga ctagctactt ctgcttgtgt tagtttcagg
     9241 tttttcatca gcatctcata tgcttctgct tcttctaacg gattcagatc ttctcgctgc
     9301 aagttttcca gcactgctac ttgcatcatc gcttcttcgt caaagtctct aacgatagca
     9361 gggatcgttt ctttttcagc tagtttggac gctcgaaaac gtcgctcccc tgcaataatc
     9421 tcatatcctt ttactgcgga acggcgtacg atgatgggtt gaaagacacc tgattgttca
     9481 atagaattag ctagttcctg caaagatact tcttcaaatg ttttacgggg ctgatacgga
     9541 tttggtcgca gctcattcaa tggtagctgg acaacggtat catcttttac atccaccgtc
     9601 tctaagtttt ctaaatcttg aaacagtgca tcgattcctc ggcctaatcc tttacttttc
     9661 ttcacgactt accacttcct ttgccaatgc ttggtagact tcagctcctc gagaacgtgg
     9721 atcgtagtcg atgatcggtt ttccatgact aggtgcttct gataaacgaa tatttcttgg
     9781 aatgatcgtt tcgtaaactt tttcgcggaa gtatttacgt acttcttcta cgacttcatt
     9841 tcccagattt gttcgtgcat catacattgt cagtaagact ccctcgatct ctaattctgg
     9901 attaaagtgt ttttggacta aacgaaccgt attcagtaat tggcttaatc cttccaatgc
     9961 gtaatactca cactgaacag gtatcaaaat cgaatcactt gctgtaaatg aattgattgt
    10021 cagatggcca agagaaggcg ggcaatcgat caaaatataa tcatactgac tgcttacttc
    10081 tgctaaagat ccttttaatc ttgattctct tgccatcatt gacgtcaatt cgatctctgc
    10141 tcctgctaat tggagagtcg caggcacaat actcaaattt tcgtgttcag tgatcaaagt
    10201 tgcctcatca attggcaatt cattgactag aacatcatag atgtctcttg tcacatcagg
    10261 ttttctgatc ccgacaccac tggtagcatt tccttgtgca tccatatcca ctagcaatac
    10321 ttttttgcca aggctagcaa gacaagcacc tagattcacc gtcgtcgttg ttttgccgac
    10381 gccacctttt tggttcgcaa cagatattat ttgtgccatc tcttctcctc ctatttgatt
    10441 ggctgcttat taggcagtcc gggctttctt gggtatttct ttggggttgg tttctttttt
    10501 tgaataacga tgatatgccg ttgatcagag acattcggca aggtaaccgc ctcttctttg
    10561 aggaatttac cacctaataa agcaatcgct tgttttgctt ctgtcaattc ctcttcactt
    10621 tttgctgctt tcaatgcata aaaatagcct tcttgtttag ccaagggcaa acacaattcc
    10681 gctaacacat tcaaacgagc aaccgcacga gcagtgacaa ggtcaaatga tgcacgaaag
    10741 tgaggatttt gcccaaatgt ttctgcccgg tcatgatata aggaagcatc gattcctaat
    10801 aatcgggtta actcagataa gaaggttatt cgtttattca gagaatcaac gatcgttatc
    10861 tgcagttctg gaaaggcaat ttttaaaggt atgctaggaa accctgcccc cgaaccaaca
    10921 tcgcataaag ataaaggttg cgtaaagtct acttcaaatg ctaatgtaag tgaatcgtaa
    10981 aaatgcttca gatatacgtc tttttcatcc gtaatagctg taaggttcat cttttcgttc
    11041 cattcgacta atgtccgata ataaatagcg aattgattca tttgatgatc ggtaagctct
    11101 atcccttttt ctgccaagga ttggcgaaat tcttctggta tcataaatat tcctttcatt
    11161 gtgaaacagc aattgtttca cagattctct ccctacttta acgcaaaatc tctagacatg
    11221 caatccttcc atatggattc ttaaaaattt gaaaagtaag aaaagccgat agactttttc
    11281 tttctagtat gactcataaa atatacacag aagagagaac tagctttgag caatttctgc
    11341 aaaaactagt tctctcttct atttattatt acgtattacg ctggttcact gactttcgca
    11401 atcttccctt gttcgatata gaccattagg atacttaggt ctgcagggtt tacgccactg
    11461 attcggctag cttgggcaat tgtttctggt tggattttct gcaatttttg ttttgcttct
    11521 gttgctaatc cgttgattgc tgcgtagtcg atgttttctg gaatcttttt cgcttccatg
    11581 cgttttaatt tttctacttt ttccatggct tttttgatgt agccttcata tttgatttgg
    11641 atttctactt gttcgatttc ttttcgtgtc aattcttcat tttctggaat aaaccgtaga
    11701 agttctgcat aagtaatctc aggacgtctt aaaaagtcgc ttgctagtac accatctttt
    11761 aatggtgccg cattaatcgt tgctaaaaag tcttggactt cttctgttgg tttgattcgt
    11821 acattcatca aacgtttgat ttcttgttca actgcttctt ttttcttcaa ataattcttg
    11881 tattgatctt cttttactaa gcctacacgg tgacccattt ctgttaatcg caaatctgca
    11941 ttatcatgac gtaaaagcag acggtattca gcacgtgaag tcagcaaacg ataaggttcg
    12001 ttcgttcctt ttgttaccaa atcatcgatc atgacaccga tgtaaccgtc acttcgtttg
    12061 atcactaaag gttcttttcc ctgaactttc aaagctgcat tgataccggc aatcagccct
    12121 tgtccagccg cttcttcata accgcttgta ccatttgttt gtccagctgt ataaagattt
    12181 tcaacgactt tagtttctaa tgtcggacgg agctggtgtg gctctactac gtcatattcg
    12241 atagcatatc ctgtacgcat catttctact ttttctaatc cagcaataga atgaagcatg
    12301 ttgacttgaa catcctctgg taaagaagta gataaacctt gaacatagac ttcttccgta
    12361 tttaagcctt ctggttccaa aaataattga tgacgtggtt tgtctgcaaa acgcacgatc
    12421 ttatcttcga tggatggaca atagcgagcg cctacccctt caacgatccc tgtaaacatt
    12481 ggggcacgat gtaagttgtc tcgaataatt tggtgagtcg tctcacctgt ataggtcagc
    12541 cagcaagatt cctgatctag tttataggca ctatctggtg tgctgaagct aaaatgattc
    12601 ggctctttgt caccaggctg ctcttctgta acagaatagt cgatcgtact tgattttact
    12661 cgaggcggtg ttcctgtttt gaaccgttcg atagcaaagc caagttcttt tagatgattc
    12721 gctagtccaa tagaaggttg agagttattc ggacctgatg aatatttcaa ttcaccgata
    12781 atgatttctc cgcgaagtgc tgttccggct gtaataacaa ctgctttggc ccggtagctc
    12841 gctcctgtgg acgtcactac tccatgacaa acaccatctt cgacgatcag ttcttcgacg
    12901 attccttgtc ttagcgtcaa atttggttct ttttcgatcg tatgtttcat ttctgttgaa
    12961 taagcatgtt tgtctgcttg tgctcgcaat gctcggacag ctgggccttt tcctgtgttc
    13021 aacatacgca tttggatata ggttttatcg atattcttac ccatttctcc gcctaacgca
    13081 tcgatttctc gaacgacaac gcctttagct ggtccgccta cagaaggatt acatggcatg
    13141 aatgctacca tgtccaaatt tattgtcagt aataatgttt ttgatcccat acgtgcagca
    13201 gcaagtgcag cttctgaacc ggcgtgtcct gcgccaacaa cgatcacgtc atacgcatct
    13261 gcttcataat gttccaatcg tttgttcctc cttttatttt cctaaacaaa attgactgaa
    13321 taattgagtg attagctcat cttgtacact gtcgccgaca acttctccta gatagtccca
    13381 gcaacgcgtc atatctattt gaacgagatc cacaggcatt cctgaatcaa tacctgcaat
    13441 gacttcttca agtgagaggg aagctttttc taacaacgcg atatggcggg tattagaaac
    13501 ataagtagcg tctctttctc ctgtttctcc gctgaagaat aaatcagaaa tagctgattc
    13561 caaacgatct agtccgtcgt ttttagctac ggaaattgaa aaaactggtt cattttcgat
    13621 caatttcttc aatttttctt gttccagctg tgctggcaaa tctgttttat ttagaagaat
    13681 gatccgtttc aatccacttg tcgcttccaa taattgttca tcttctgctg tcagtggttc
    13741 gctttggttt agaacaagta agatcaagtc tgcttctgct aatgcttttc ggcttctttc
    13801 gaccccgatc cgttccacca catcttctgt ttcccgaatt ccagctgtat cgattagctt
    13861 gagcggtacc ccccgaacat tgacatactc ttcgattaca tctcgagtcg ttcctgcaat
    13921 atccgtaacg attgcttttt cttctcttaa caaatggttt aacaaacttg atttgccgac
    13981 atttggccgg ccgatgattg ccgtacttaa cccttcccgc agaaccttcc cttgcttgga
    14041 tgtagcaagt agcgcttgga ttctttgttg gatcatctgc gctttttcaa gcaataactt
    14101 cgtagtcaat tcttcgacat catcatattc tggataatca atatttactt ctacttgtgc
    14161 taatgtctct aaaatctctt gtctcaatga gcggatcaaa gcagacagat tgccgtctaa
    14221 ctgattaaga gctagtccca ttgctttgtc tgtttttgcc cgaatcaaat ccatcacagc
    14281 ttctgcctgt gaaaggtcaa cacgaccatt caagaatgct cgttttgtga attcgcctgg
    14341 ctcagctaat cttgcacctt ctcttaacaa tagctgcaaa atttgattga ctacaacgat
    14401 cccaccgtga caattgattt ctactacatc ttcccgcgta aatgtctttg gtgctctcat
    14461 cacggagacc atgacttcat cgacaagctg attgctcttg ggatcaacga tatgtccata
    14521 atggatcgta tgacttggta cttctgataa tcgtttgttt ccagattgat aaactttatc
    14581 tgctagttgc acagcttgat cgccacttaa acgaacgata ctgattgcac cttctccagg
    14641 tggggtagaa attgccgcaa tcgtatcaaa ttctaaagta atttcagcca ttttttcact
    14701 tcctttttca ttttgcgcac aaaaaaagtg cccactccac cctttatgca caataaacgg
    14761 tgaacttcgc actttgactg cttgttcttg attaaattct ggtcatagat tatcacactt
    14821 gctataagga atcaagtgtt taaaacaatt agatgaagtt gagcctgttc tgcttccatt
    14881 accttatatt taattgtcct tggatgtctc tttgcgttct ttggtttatt cgaagagaag
    14941 atactgtata aacaaggctt tatctttctt aaaaaaaacg gtacactaaa tggtagcgct
    15001 atctaaacta tacacttttg aaaggagaaa atatgtgagc ccgttcagat ctgctgcatt
    15061 ggaagaagaa agtgaccctc ggatcaacga tgtcgtttta tctacaggga atatcaacca
    15121 gaaatatatc gtccgcgata tcgtttttgt tgtggaatgc ttagaagctg atttgtttga
    15181 cccatctttt cccaaagatg accttttctt aacaacgaag aggaaattaa agacgaaggt
    15241 acttgaatat ggtgcgaatg cagtaatcaa ctgtcatttt gagtatcagc aaaaaaaacg
    15301 cgacggaaag tattttctgg aactttttgc ttatggaaca gtgatccaat ttatccaaac
    15361 aacgattggt taaatgaaac agacaaaaaa agcggatcaa aactgaagac agtttcgatc
    15421 cgcttttttc tatcaaagtt agtgactact tcatcgtata ctctgataag aatgatttct
    15481 agtaaaatat acttggtttg aaaaactttg tcaacgtgat ttattcaaaa agtaatggaa
    15541 tcaataatat tttttagcag gttctactac taaataacga taaggttcct caccttccga
    15601 atgtgttttt acatgttcat ctttgcttaa tgcagcatgg atttgtttac gttcaaatgc
    15661 aggcattggt tctaagaaaa caggctgccc tgtccgtttt actttttcag ccgtgcgatc
    15721 agctaaacgc tgaatgatct cttggcgctt ttcacggtaa ttgcctacat tgactactac
    15781 agacagctta tttcttgcta aacgatggat gaagacttgt gccagatatt gcaatgcatt
    15841 cagtgttttt ccatgtttcc caatcagtat gccttgcttt tgtgtttcta aatggaaaac
    15901 aaccatatta tttgattctc tttccatttt gacaagagct ggagcatcaa gctgttttga
    15961 aatttcagta agataaactg ccaattccgt taaagctgct tcatcaccta gctcacttac
    16021 ttcttctgtt ttttcttcaa gaatcgtttc gttgactacc tcgatctcag ctgttggttc
    16081 ttcttccaaa atttcttcta cagtctgttc tactgtttca gaaacttttt cgctgatact
    16141 tggctggatc gaaagccgtg cattctttcg acccattcct aggaaacctt tttttccttc
    16201 ttccagtatt tcgatttcga cttggtcttt ggccaaaccc aaatcagcca gacctagttg
    16261 gacagcttcg tctattgttg cagcttcata aatcggcatt agcattacct cccctattat
    16321 ttccgttttt tcttaggatt cattgctttc ttaagagcac gttctctttc gcggattctc
    16381 cgtgcttctt cttcacgctc ttttcgaatt ttgaaagggt tgttgatcag cagcgtttgt
    16441 acaacttgga aggcattgga aactacccag tataatgaaa gaccgctggc taaattgaca
    16501 cccatcacta aaatcattac aggcatcacg aaattcatga tcttcattga tgcattcgat
    16561 tcaatttgac tcatgctaga taaatacgta cttgcaaaag taaagattgc tgctaaaatc
    16621 ggcaaaataa atgttggatc cgcgttccct aagttcaacc ataagaaatg cccttgtttc
    16681 aactctggta cacgtgaaat agattgccat aacgccatca agattggcat ttgtactaat
    16741 aaaggaagac aaccagcata tggattgacg ccattttctg catataaacg ctgctgctct
    16801 tctcttaact tactttgtgt ttccggatct cttgaactat attgttgctg cagtgctttt
    16861 aatttcggtt gtaattcttg cgttttacgc atgctctttg tttggaaatg catcaacggc
    16921 aataggatca cgcggatgat cagtgtaaat aaaatgatcc ctatccctgc attaccaaat
    16981 gacaacgcct tgatcgcctc agcaaagtaa taaacaatat aacgatccca aattcctgta
    17041 ctctgtgcgc tgacttcccc agttccgcaa ccggataaga cgatgaccaa actaacgata
    17101 ccggccatca aaagtaaacg tttgtatttc ttcacttact cttttcctct ctgtcgatta
    17161 tttttgcaag ttttaagacg tggactaaat tggagcgaac ctcatcataa gtcaacccct
    17221 tcacacttgg tctagcaatc acaataaaat ccaactccgg atcaatttct tcttttatag
    17281 aataaactgc tgctcggatt tttcgtttga cttcattgcg gtttaccgcg ttgccgacct
    17341 tttttcctac agataaacca actcgaaaat gaggctgtcc cggtttttcc aaacggtaaa
    17401 cgacgaattt gcggttagca aaagaatctc ccgtatggaa cacggcttga aactctcgtt
    17461 ctttttttac tcgatacgct tttctcatta gttttctttc cttttctatc tctcacttac
    17521 gctcataatc ataccataat tacgattgtc ctttcatcat ttccctaaaa aagagtataa
    17581 aaaaaaccac tgaaacccca gtggcctaag ctgaaagaac ttttcttcct ttgcgacgac
    17641 ggctagctaa cacacgacgt ccatttttag tactcatacg tttacggaat ccgtgaactt
    17701 tttgacgttt acgtttattt ggttggtatg ttcttttcat atattccacc tccaaaatgt
    17761 tcttatctat tttataaaca cagacatact ttgatagtat aacgacaaag tagtgatttt
    17821 gtcaatataa aatagaagat cgattgttaa aacttatcca caagattatt tttttctatg
    17881 ggaatcctat gtggaaaaag ttatgcacag ggattatcca cagataataa aaggctggtt
    17941 ttacacagaa aacacacgtg taaaaaaagt aatccacaat caggcgattt cctgtgtatt
    18001 ccgtctccta actattggta tatctgtatt ttttatccac agacgcaaga ggaaatctat
    18061 tggtttttac gagttttcca cctgtgcatt agtggtgtgg aaaacttttt ttgacaactg
    18121 tgtttttctc ttttttcttt ttgtcgattg tgtggataat tgtaattaaa aaattttttt
    18181 cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat tagtactagt tattcacttt
    18241 ttaatgaagg aggaggcctt atatggtatc cctcgatgct ttatggaatg aattaaaagc
    18301 aacataccaa aaggatttat cgcctgctag ctataataca tggatcgaaa cagcgaaccc
    18361 tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct agcaagattc ataaagaata
    18421 ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt tatatgttat caggaaatga
    18481 gattatccct cgctttatta ctggcgaaga agcagagcaa gaagaagtta tagaagaaaa
    18541 aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc atgctgaacc ctaaatatac
    18601 ctttgatacg tttgtcattg gtaaaggaaa ccagatggct catgcagcag cgctcgttgt
    18661 agcagaagat ccaggttcta tttataaccc gctcttcttt tatggaggcg tgggacttgg
    18721 taagactcac ttgatgcatg caattggtca tcaaatgctg caaagtcagc ctaatgccaa
    18781 agtaaaatat gtaagcagtg aaacttttgc taatgatttc atcaattcga tccaaaacaa
    18841 aacagcagaa gagttccgtc aagaatatcg aaatgttgat ttactgctag tggatgacat
    18901 tcaatttttt gcagaaaaag aagctacgca agaagagttt ttccatactt tcaatgcctt
    18961 gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg ttgccaaacg agattccgaa
    19021 actgcaagaa cgccttgttt ctcgctttgc atgggggctg tctgttgata ttacaccgcc
    19081 ggatcttgaa acaagaacag cgattttacg taaaaaagcg gcggctgaac gtctagagat
    19141 cccagatgac acactaagct acatcgcagg acaaattgat tctaacattc gtgaattaga
    19201 aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat agtgaagata tttcgactag
    19261 tctagctgca gacgcactga aaacgttaaa atcaggaaaa ggacatcctc aattgtcaat
    19321 cctgcagatc caagaagaag tggcaaagta ttatcacatc caattgaaag atctcaaagg
    19381 aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc gctatgtatt tggcacgtga
    19441 gctcacagat aattctttac cgaaaatcgg agctgaattc ggtggcaagg atcatacaac
    19501 ggttatccat gctcatgaaa aaatccagca gctgatggat tctagtgtca gtatgcaaaa
    19561 tgaagtatca gaaatcaaaa actttttact aaattaattt gtcgattcta tcaactgaaa
    19621 atagcatgtg gataaaagaa aaaaacgttc aaaagttata cacacttttt ccacagctgt
    19681 tttttcgttt ctctcttacg ttttttgagt tttccacaga atcaacatgc cctattactt
    19741 ttattattct ttttaactaa taataaataa acaaacattc aaaggagata catcatgaaa
    19801 gttactttaa accgagctag ctttatgcag gaattgcaaa ctgttcaacg agctatttca
    19861 agcaaaacca cgatccctat tttgacaggt gtaaaaatca cactgacaca agaaggtttg
    19921 actttgacgg ggagcaacgc tgatatatca attgaaactt ttttgtctgt tgaaaacgaa
    19981 aaagcaaata tgcaaatcga atctactggt tccattgttt tacaagcacg tttctttagc
    20041 gaaatcattc ggagacttcc tgaagaaaca tttactttag aagttttaga aaataaacaa
    20101 gtagcgatca cttctggaaa agcgaatttt atcgtaaatg gattagatgc agataactat
    20161 cctcatcttc ctgttgtcga aagccataac cagatgaaat tacctgtaca cgtattgact
    20221 aaactaatca acgaaacagt ttttgctgtc tcacaacatg agagtcgtcc aatcttgaca
    20281 ggtgtgcatt tcattttatc tgataattct ttattagctg tagctactga ttctcaccgt
    20341 ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc attttgatat tgttattcct
    20401 ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg aagaagaaat cgtcgaaatc
    20461 agcattatgg aaaaccaagt gttgttcaaa acagaaacga tgtatttcta ttcccgtttg
    20521 ctagaaggaa actatccaga taccaatcgt ttgattcctt caagttttaa tacagaggtt
    20581 gaattttctg ttcctagctt tttagcggcg atcgaacggg cttctttact ttctcatgaa
    20641 ggccgtaaca acatcgttcg tttatctatt cgtccagatg cggttgtttt atacggaaat
    20701 tcgccagaaa tcgggaaagt agaagaaagc ttgagttata cagcaagcag tggcgatcca
    20761 ttagatattt ctttcaatcc ggattatatg aaagcagcgt tgcgtgcatt tggagatatg
    20821 agcatcaaag tgaaatttat ctctgctatt cgtccattta cattggaacc aacggaagat
    20881 ggcgttcaat ttattcagct gattactcct gtacgtacaa attaacaatg attttcaatg
    20941 ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa ttttaaacag aaggccctaa
    21001 a
//
LOCUS       DAZKCZ010000039        20445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000039 DAZKCZ010000000
VERSION     DAZKCZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.039"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            342..1028
                     /gene="rnc"
                     /locus_tag="ACG8KN_001359"
     CDS             342..1028
                     /gene="rnc"
                     /locus_tag="ACG8KN_001359"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9188425.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            1048..4629
                     /gene="smc"
                     /locus_tag="ACG8KN_001360"
     CDS             1048..4629
                     /gene="smc"
                     /locus_tag="ACG8KN_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFP9188426.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            4638..5447
                     /locus_tag="ACG8KN_001361"
     CDS             4638..5447
                     /locus_tag="ACG8KN_001361"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9188427.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            5458..6456
                     /gene="ftsY"
                     /locus_tag="ACG8KN_001362"
     CDS             5458..6456
                     /gene="ftsY"
                     /locus_tag="ACG8KN_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9188428.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            6643..8910
                     /gene="gshAB"
                     /locus_tag="ACG8KN_001363"
     CDS             6643..8910
                     /gene="gshAB"
                     /locus_tag="ACG8KN_001363"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFP9188429.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(8999..9451)
                     /locus_tag="ACG8KN_001364"
     CDS             complement(8999..9451)
                     /locus_tag="ACG8KN_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFP9188430.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            9601..10551
                     /locus_tag="ACG8KN_001365"
     CDS             9601..10551
                     /locus_tag="ACG8KN_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9188431.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            10544..11503
                     /locus_tag="ACG8KN_001366"
     CDS             10544..11503
                     /locus_tag="ACG8KN_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9188432.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            11500..12255
                     /locus_tag="ACG8KN_001367"
     CDS             11500..12255
                     /locus_tag="ACG8KN_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188433.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            12278..13231
                     /locus_tag="ACG8KN_001368"
     CDS             12278..13231
                     /locus_tag="ACG8KN_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9188434.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(13875..14171)
                     /locus_tag="ACG8KN_001369"
     CDS             complement(13875..14171)
                     /locus_tag="ACG8KN_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFP9188435.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            14526..14882
                     /locus_tag="ACG8KN_001370"
     CDS             14526..14882
                     /locus_tag="ACG8KN_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9188436.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            14892..15791
                     /locus_tag="ACG8KN_001371"
     CDS             14892..15791
                     /locus_tag="ACG8KN_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9188437.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            16311..17039
                     /locus_tag="ACG8KN_001372"
     CDS             16311..17039
                     /locus_tag="ACG8KN_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9188438.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            17184..19211
                     /locus_tag="ACG8KN_001373"
     CDS             17184..19211
                     /locus_tag="ACG8KN_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFP9188439.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            19319..20416
                     /locus_tag="ACG8KN_001374"
     CDS             19319..20416
                     /locus_tag="ACG8KN_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9188440.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         6888 a         3495 c         4300 g         5762 t
ORIGIN      
        1 aaaatgaccg aaaaagggcg atgtcctttt tcggtttttt gatatttttc tgtttatttc
       61 tctaatattc aggtcaaggc gctgatttca tgacttctcc aataaacggt gttcaaagtc
      121 cagtttctcg gtattaagtc aaatttatgc aaaaatatgg aaagccattt tcacaaaatt
      181 ttttcttcat actcgaaact aaacgaattt ttcacaactt ctcgtcaatc actaggatta
      241 ttaaagcttt acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat
      301 aaggaagtat cgaaaaaata tccataaaag gaatgtggaa aatggacaac cagctgacaa
      361 aagaactaaa agaaaactat aacatcgttt ttcacgatct aaatttgctt gaacaagctt
      421 ttacccattc atcctatgtg aatgagcatc gcaatctgca attatcggat aatgagcgac
      481 ttgaattttt aggagatgcc gttttagaac tgatggtttc tcagtattta ttccgtttat
      541 atcctcattt gccagaagga aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca
      601 gcttgtcgaa atttgccaaa gaatgtcact ttgatcaata tgttttgttg ggcaagggag
      661 aagagaattc aggaggacgt acgcgaccag cattgctttg cgacctattc gaagcttttt
      721 taggcgcgtt atatcttgac cagggattcg acacgaccca tgcattcatc gaaaaagtga
      781 tttttcctaa agtcaaagca ggtgcttttt cacatgagat ggatcataaa acaaaactac
      841 aagaagtctt gcaaaaatca ggtgatgtat caatcgaata ccgattgatc aatgaagaag
      901 gtccagctca tgaacgagta ttttggatcg aagtttatgt agatgatcaa ttgattggca
      961 ctggacaagg gaaatcgaaa aaattagcag aacaagctgc ggcggaaaat gcattggcag
     1021 cactgtaaaa gtaagtgagg gaaagctttg tatttgaaac gaattgaaat agccggtttt
     1081 aaatcatttg cagacaagac agtcattgat tttgaaaata gcgtcacagc agtggttgga
     1141 ccaaatggca gcggaaaaag caatattaca gaggctatcc gctgggtatt aggagaacaa
     1201 tctgctaaaa gtctgcgagg cggaaaaatg ccggatatca tctttgccgg ttccgatacc
     1261 cgtaagcagc tgaatattgc agaagtcaca gtgattttag acaatagtga tcattatctg
     1321 ccattggagt acaatgagat cagtgtaacc cgccgttatc gacggactgg ggaaagcgag
     1381 tttttcatca ataaacaaag ctgcagatta aaagatatcc aagaattgtt cttagattct
     1441 ggattaggaa aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc gattttcagt
     1501 agtaaaccgg aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt gaaatacaag
     1561 cagcggaaaa agaaagcaga acagaagctt tttgaaacag aagacaacct cagccgcgtc
     1621 caagacatca tccacgaatt ggaagaacag ttaactcctt tggccgccca gagtgaggca
     1681 gcaaaggagt ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt
     1741 gctgaaatca agacagcaaa aaaagattgg gacaacaaac aagcacagct tgcgaaattc
     1801 aatctagaat taggcaaact atctgagtcg atccaagaac aagaatccat tttggcgaag
     1861 cagcgtaaag aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca agtattatta
     1921 gatctttcgg aaaaactgaa gcagacagaa ggacaaaaag atgttttgca ggagcggaca
     1981 aagcatacac aaaaaagctc gcaagaatat caaacttcat tagcagaagc acaaaaaaag
     2041 gttaaacatt ttgaaaagct tcaagaaagc ttgatgaaag cagcagcaga aaaagaaaca
     2101 gagatccaaa aagctgaagc aaatctcata aaaacacagc aagaattaga aaaataccaa
     2161 aaatcaacga aagaattatt ggcagaatta agagatcaat atgttgattt gatgcaagaa
     2221 caagcagctg taggaaacga actgaagtat ctagaacgtc aatatataca agaaacagca
     2281 aaaagcaagc agacgttagc caagcaatcg gaagtagaag catcagtaga ccgattgatc
     2341 ttacaaaaag aagaattgac acaaaagcag gcgcagctaa aatcatcttt aactgaaaca
     2401 aaagagaagt tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa
     2461 gaacagccta aaatgtatca attaatgaat caagtccagc agctacgtgc tcgtaagaaa
     2521 agccttcaag aaatccaaga aaactatttt ggtttttacc aaggggttcg attggtcttg
     2581 cagcataaac agcagctttc gggtattgtc ggagcagtgg ctgaactgat cgatgttcct
     2641 gcagatttca cgttagctat cgaaacagct ttaggtgggg cagcccagca tgtcatcgtt
     2701 gaaaatgaaa aagatgctcg gcaagccatc acgtatttga aacagcaaag aggaggaaga
     2761 gcaacattcc ttccactgac gacaatcaaa ccaagacaat taccggctca tatattgaca
     2821 caagcagcag ctgtagaagg gtttataggg attgccagtg aacaagtcag ttatcccgat
     2881 caaatccaaa ctgtggttca aaacctgcta ggaacgatcc ttttagccaa agacttaact
     2941 agtgccaatg caatcgcgca aacgatccgc tatcaatatc gagtggtttc cttagaaggt
     3001 gatgtgatga atgccggagg atccatgaca ggtggagcga ataaacgagg aaaccaaggc
     3061 agtttgtttg tacaaaatca agaattgaaa cagttgacta gtgaatttga agaagcagat
     3121 aaacaactcc aagcacaaga aaaaaaagtt caggagctgc aacaagaaac agctcgttta
     3181 gccgaagaac aagaagtttt gcgtacacga ggcgaacaat tacgttttga agaacaagaa
     3241 gcgacaaatc agctacaaaa catcataaat gaacttgaac gttttgaaaa agaaaagcaa
     3301 atctcaactt ttgagacacg tgaactgcag cagtttatcg aagattacga aaagcaaacg
     3361 aacgaattga aagacaaaca aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa
     3421 atcaaatcgt taagtcaaga aagcgaccag atggaagcca gaagagcaca agtacaaagt
     3481 caaaaagcac aagaacaagc agatcttgct gtcttgaaag agcagtttaa tcatctgcag
     3541 atccagctcc gcggtgcccg agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata
     3601 gaaaagcagt tggcgacatt gacagcagac ttttctgatc atgaagtcac agaagaaagt
     3661 ttggaaaaac aaattaacga attatctgct cagcgagaaa cattaaaagc cgagttggca
     3721 aaagccaaag agcagcgaga tagaaggcaa aaggagatcg ataagcttga agcagtcttg
     3781 gcagagcgta atcgtgagca aaaagcacga ctgtcggaac aatccaaatt agaagtccaa
     3841 aaagaccgag ctgaaatgat tttagataat catttgacct atctgcagtc agaatatcag
     3901 ctgacatttg agaaagcttc ccaagactat caagaaacaa ccgatatcga agacagtaga
     3961 acaaaggttt catccttaaa agaacagatt gaaaaactgg gaccagtaaa cttaaattca
     4021 atcgaacaat acgaacaagt cagtgaaaga catacattct tagcaacaca gcgagatgat
     4081 ctgttagcag cgaaaaacca attgtttgaa acgatggatg agatggatga tgaagtgcgg
     4141 acaagattca aagaagtatt tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat
     4201 atgtttggcg gcggtaaagc tgaattagta ctgacggaac caactgattt gctgaaaaca
     4261 ggtattgaga ttgaagtaca acctcctggc aaaaaactgc aaagtctaag tttgctttct
     4321 ggaggagaac gagcacttac agccatcgcg ttgctctttt ccattatccg tgtctgtcct
     4381 gttccgtttt gtatcttaga tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt
     4441 tttggtcgtt acttgagcga tttccaagat gacacacaat ttatcgtagt cacacaccgc
     4501 aaaggaacga tggtcgcagc agatgtactg tacggagtga cgatgcagga atccggtgta
     4561 tcaaaaatcg tttctgtacg gatggaagat atcaatgaag aggcgaaaat caacgaaacg
     4621 aaagtgtagg aacgaaaatg atcaaattag tagcaatcga tttggacggt acattattaa
     4681 atgaacaaaa gctgatttca gatgagaata aacaagcatt agcacaagca aaacagcaag
     4741 gggtcaagat cgttctatgt acaggacgcc ccttagctgc gatggcgcat tatctgcaag
     4801 aattaggact ggtagatgaa ggtgactaca gcatcacttt caacggcggt ttggtacaaa
     4861 aaaatgatac tggtgaaatc atggaaaaga aagtgatgga agtatcagat atccaacgct
     4921 tatacaaact ggctcagcaa ttggatctgc cactagatgt tttgtctgac aatgtggttt
     4981 tacagctgcc tagtgcccca aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc
     5041 aattccaacc agcggatata agcgatatta cagatgaatt tgtgcttaat aaggcagtaa
     5101 tcgcttatga ccaagaagaa ttagatccta aaatcaaaga aataccagca gaattccatg
     5161 aaatgtatga gatcatcaaa acaagaagta tgcttttgga atttatgcca aaaggtgtta
     5221 caaaagctta tggtatttct ttattggcaa aagatcttgg tttgaaacaa gaagaaatca
     5281 tggcgatcgg tgatgaggaa aatgatttac cgatgatcca atatgctgga ctaggcgtag
     5341 cgatggagaa tgctgtgcct ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg
     5401 aagaaaatgg cgtagccaaa gtgatccaaa aatttgtatt agagtagggg gagtcaaatg
     5461 ggattttttg ataaaatcaa gcgtgctttt actggcgaag agaaaacaga agaagttgaa
     5521 gtacaggaga agtacgacaa gggtttagaa aagacgcgta aaacatttgg tgaacggatg
     5581 aatgagttgt ttgccaattt tcgaacagtc gatgaagact tttttgaaga attagaagaa
     5641 acacttatcg gtgcagatgt aggttttgaa acagcgatac gaattaccga agcattacgt
     5701 caagaagtga aactcagaaa tgcgaaaaaa tcggcagaag tccaaaatgc gatcatcgag
     5761 aaacttgtgg atctctatga agcggaagga atcaatgaag taaatgaact aaatatccaa
     5821 gaagacggac taacagttat tttgtttgtc ggtgttaatg gtgtagggaa aacaacaagt
     5881 attggtaaac ttgcccatca gttcaaaaac gaaggtaaaa aagtattgat ggcagcagca
     5941 gataccttcc gcgctggagc aatcgaccag ttagttgtct ggggcgaacg cgctggtgtt
     6001 gaagttgttc gtggcaatgc aggaggcgat ccagcagctg tcgtattcga tgctttggaa
     6061 agagcaaaga cagagcaggc agatgtttta ctagttgata ctgcaggccg actgcaaaat
     6121 aaagtgaact tgatgaacga attagaaaaa ataaaacgag taatccagcg ggaattacca
     6181 gaagcacccc aagaaacact tcttgtatta gatgcaacca ctggtcaaaa cgcgatggtt
     6241 caagcaaaac aattcaaaga aacaacagat gtgaccggtc ttgttttgac aaaattagat
     6301 ggtacagcaa agggcggtat cgtacttgct atccgtaatg aattgcattt accagtgaaa
     6361 ttggtcggtc ttggtgaagg aatcgatgac ttggagccgt ttgatccaaa tgattttgtc
     6421 gttggattgt tcaaaggatt actaaaagaa gaataaccca tgtctaaaaa gcaggagctg
     6481 tgacaaaagt tgcagctcct tgtttgaatg attgttttta acatagctgt gtttttgatg
     6541 ccttcatttt tatacttatt tgtaagcggt tctcaaacaa tttcgttctt tttcttagac
     6601 tttttgatta taattagatt catatgaatt ggaggtcact taatgatgaa ttttaagcaa
     6661 ttattattgc atgtcaatgc gcgccctttt atcgaccaag ctcgatttgg tatagaacgg
     6721 gaaggacagc gggttgatct tgcaggaaat ctagcaaaaa ccgatcatcc agcaatcttt
     6781 ggcgatcgat cctatcaccc ctatatccaa acagatttta gcgaaacaca aacagagatg
     6841 atcacccctg ttaccgattc tattcccgaa ttatttcagt atctcgctgc tgtttatgat
     6901 gtgactgctc gttctatacc gaaagaagag atgatctggc cattaagtat gccacctgcc
     6961 ttaccggaaa aagacgaaga gatcattatt gcaaaattaa aaaatttcga agatgtcttg
     7021 tatcgacgtt atttagcaaa agaatacggg aagcgaaaac agatggtaag cggtattcat
     7081 ttcaattttg aatttggcga tgagttgcta agaacattgt tcagccatca ggaagaattt
     7141 caagattttt ccgaattcaa aacagaactt tatttgaaaa cagcgagaaa ctttatgcgc
     7201 tatcgctgga tgatcactta tttattcggt gcttcaccaa tgagtgagaa gaactacttt
     7261 ttagatgaat cacatccgca agaacctgtt cgcagtattc gcaacagtgc gctgggctat
     7321 acgaatcatc caaatgtgaa agtttcctat gcttctatga aacagtattt agcagatatc
     7381 gagcgaatga tcgaagaagg gaaactttcg gaggagaaag aattttatac accgcttcgt
     7441 ttccgaggcg gaaagaaagt cgcagattta gcaacaacag gtgttcgtta tattgaattg
     7501 cggaatatcg atttgaatcc ttatgcaaga ttgggtatca atccagagca agttcggttc
     7561 ttacaattat tcctgatgta catgttatgg acagaagaaa aagaagactg tgaccaatgg
     7621 gtagcagaag gtacgactcg aaataacaaa gtagcactag aacaaccatc agatcaaaca
     7681 gaatttcatc aagaaggtag agaaattctt gaaggaatga agcaaatgct ggttgaattg
     7741 gattggttgg attctcttta tttagtagag gaagcattga ctcaaatgga tcatcctgaa
     7801 caaacgttag cagcgaaact ctatcaagaa gcacagctat cgagtcagca agaagttgct
     7861 gtggcattgg gacatcaata ttataaagaa agccatgaac gtccctatca gttggccggt
     7921 tttcgcgaaa tggaactttc tactcagata tttatgttcg atgccatcca aaaaggtgtc
     7981 caagtcaaag tattagatga gtcggatcaa tttttgcgtc tgcaattcca agaccatgta
     8041 gaatatgtga aaaatgcaaa catgacaagt aaggacagct atatcgtccc acttatcatg
     8101 gaaaacaaaa cagtaacgaa aaaagtctta aaagaagctg ggttccgggt accaggaggc
     8161 gcagaatttt catccatgga agaagcggta aaagcttatc cgagatttgc cgagcaggca
     8221 tttgtgatca aacctaaatc aaccaattac gggttaggta tcacgatttt caaggaagga
     8281 gctagtctgg aagattatca agcaggttta gccattgctt ttcgtgaaga cagttcggtt
     8341 ttagtagaag aatttatgcc gggaacggaa tatcgtttct ttgtgatcga cggagaagtc
     8401 caagccatca tgttgcgagt ccctgctaat gttattggtg acagtatccg aacagtgaaa
     8461 gaacttgttg aagaaaaaaa tagtgaccct ttgcgtggaa cgaatcatcg tgcaccttta
     8521 gaattgatcc aattaggtga attagaacaa ttgatgttga aagaacaagg gttgacgatc
     8581 gaatcagttc cccaagccaa tcaaattgtt tatctaaggg agaactcaaa tatcagtaca
     8641 ggtggtgatt cgatcgatat gactgatgaa ttttctgaag cttacaaaaa aatcgccgtt
     8701 tctgcggtag aagcactagg agctaagatt agtggtatcg atttgattat accagataaa
     8761 gaaatcgatc ctacaactga taaaaaagca tatgggatca tcgaagcaaa tttcaatcca
     8821 gccatgcata tgcatgttta tccttttgca ggaaaaggca gaagattgac gatgaatgta
     8881 ttgaaattgt tatatcctga agtattttaa aagaaaaagc ttttgacaga ttgtcctatt
     8941 tgatgtcaat ctgatcaaaa gctttttttg catgtaaaaa tttatgtgtt ttttcgtttt
     9001 aaaataaacg atcccagaag ctttttttct tttttggttt ttctttttca gctggcgaag
     9061 agttttcttc tgggtatttt ttttcgatat caatcacagg ttcatttact gcttcttttg
     9121 ctgttaagag caatccgaat tcctcaggat ttgcagtgac gtaatcgttg actacagtga
     9181 aacggctatt gtttttcgtt gctagctgga tatattggtt ttggacagaa aggggtagag
     9241 ccccattcaa gagtatggaa gcatctggat gttttttgag ttcttttaaa aagttttctt
     9301 tgttttttgg ttggatcatt tcagcaacag tcatactaag atagcagcgt tccctgaatg
     9361 tccctaaata tttatgctgt tcttcagggt tcactaaagg tgtaccataa agtcccttat
     9421 ccaaatggct ttttacatca tcttgtgcca taaaaaatac cctccttctt ctctttgtct
     9481 ttcttacgat tatagcatat tcccaataat acctaaaaca tatgaaaaag cggttgcttt
     9541 tgttcaacga ttcacttaaa ataagaatga aaatcattct caattagaaa ggtggattgg
     9601 atgcgctacg gacgactagt agcattatta gttattttaa gtagtatttc attgttcatc
     9661 ggagtatcga atgtacaatt gtcagaactg ctccagttag atgggcaaag cttacaaatc
     9721 ttgctagtga ctcgtttgcc acgaacgatt tgtttgatcc ttgtgggagc gaccagtagt
     9781 atttgtgggt tgatcatgca acatttgacc caaaataaat ttgtttcacc aacgactgcg
     9841 ggcacgatgg atagtgcgcg tttaggtatt ttagtggcta tgattttcct acctggtgca
     9901 tccttgattg tccgatcctt gacagcattt tgctttgctt ttgcaggaac attgcttttt
     9961 ttatccctta cgcgattgtt cctccagaaa aatcaattga tgcttccgtt gattggagtc
    10021 atgttaggga atatcattgg ttcagttgct actttttttg cttatcaatt ccagttggta
    10081 caaaatatga cttcatggct tcaagggaat tttgcgactg tgatacgagg aaattatgag
    10141 ttattgtatg ctgttgttcc attgttgctc ttggtttggc tatttgctta ccgatttacg
    10201 atcattggtc tgggcgaatc gtttgcagta aatctgggaa tagattacca gaagatgcag
    10261 tttttaggca ttggcttagt atcattggct agtgcagtca tgttgatcat ggtaggaagc
    10321 attcctttta tcggagtgat cattccgaat cttgtctcta gaatatatgg agatcaagtg
    10381 cacaaaacaa ttggcgtaac tgctttagca ggtagtttgt tcttagtatt ttgcgatctt
    10441 gtcgctcgcg tgttgatttt tccttatgaa ataccagtca gtgtgatcgt tggaatcatc
    10501 ggtggggcta tcttccttta tcttctgata aggggacgac gatatgctta aaaaaaagcc
    10561 atctattttt attggtatgc ttgttcttgt tttatttgtt acaggaatgt acttgacgat
    10621 ccatacatat ggaaactggc attttgcatg tgcattgagg agtaagaaaa tttttgcatt
    10681 tatccttgtc tcgctagctg tcagcagtgc cacaatcagc tttcagacaa ttacgcataa
    10741 ccagtttttg actccttcag tattgggact tgagtcgtta tatgtccttg tccaaacggt
    10801 ccttttcttc tttatcggag gagtgacgat gcttcagcag acaggtacag tttttttctt
    10861 attgaacgtt gggctgatgg tcgggctgag tctattgtta tttttctttc ttttgaaaaa
    10921 aatgagagcg aatctttttt tattactgat gtcagggatg attttaggta cgctttttgg
    10981 tagtgtcagt acctttttac aagtcatcat ggatccgaat gaatatgatt tgcttcaagg
    11041 cagattattt gcaagctttg gcaatatcca gagtcagtat cttttgcctg cttctttgat
    11101 cattatcggc tgctggtacc tgttatttcg atatcgacat gtgctagacg ttttccatct
    11161 cggaaatgaa caagcagtaa acctcgggat agacacagat cgttttgaat ttttgatttt
    11221 atgttgtgtc agtcttttga cagggacatc aacggctctt gtgggtccag ttactttttt
    11281 agggtttatt gtcgcaaata tcagttatcg tgtcttttct acttatcgtc atggtcagct
    11341 atttatcgga agctttctgg tagctatgct gtttctgaca ggaggacagc tcgcagtaga
    11401 gcagattttc ggctggaata caacagtgag tgtcgtcgtt gaatttgtcg gaggaattta
    11461 ttttgtcaca aaattgattc gcgaaaggaa gagtatccta tgattgattt acaaaaggtc
    11521 aataaactgt accatggaaa atcagtggtt aaagatgtac agttaacgat tcctgaaaag
    11581 aaattgactg cttttatcgg tccaaatggt gctggaaagt caacggtgtt gtcgatgatc
    11641 agtcgtttga taccaaaaga tacaggtgaa atctatttag accataacga agtaaaggct
    11701 tggaaatcca atgaattagc caaaaaatta tcgatcctac gacaatcgaa tgaaataaat
    11761 gtccaaatca ccgtgaaaga attagtagaa tttggacgat ttccttatac gaaaggaaaa
    11821 ttgacgacaa aagacgagga aatcgttcag gaatcactgg aacaccttgg gttagaagaa
    11881 atagcggatc aatcgattga cactctttca ggcggtcaac tccaacgtgc gtatattgca
    11941 atggtgctag cgcaagacac ggattatatt ttactggatg aacctttgaa caatctagat
    12001 atgaattttg cggttcaaat catgcagatt ttaaaaaatt tagtacaaga aatcgggaaa
    12061 acaattgtta ttgttcttca tgatatcaac tttgctgcaa gttatgcaga cgaaattgtt
    12121 gcaatgaaag atggacagtt atttacgcaa ggaacgaccc aagaaatgat aaaaaaagat
    12181 gtattggatg aattatacga aatggacata cggatttgtg agattgaagg ccgcagattc
    12241 tgtctttatt tctaaaacta acattaggag agaagagatg aaaaaattag tagtaggtat
    12301 cttgattcta gcatcatttg gtttagcagc atgtggaaat agcaacaaca ccagtcaggc
    12361 agacacaaaa aaaagcagca cacaaactga aactacattg acgataacag atagtaatgg
    12421 agaccaaatc gaggtgccaa ataatccaga aaaagtcgtc gtattcgaca atggttcttt
    12481 agatacgatg gatgctcttg gtgttggaga taaggtagtt ggagcagcta caagcagcct
    12541 tcctgagtat ctttcatcct ataaaaaagt agaatcagca ggtggaataa aagaaccaga
    12601 cttagaaaaa attaatcagc ttcagccaga tctaatcatc atttctggcc gacaaagaga
    12661 ttttcaaaaa gatctaagtc aaattgctcc gaccattttc ctttctttag atgcgaaaaa
    12721 tccatgggaa tctttccagc aaaatgtcac tgctttaggg gagatctttg gtaagcaaga
    12781 agaagctaaa actcaattag aggaattatc tagtgcaatc gatcagacga aaaagaaagc
    12841 agaagctact gacaaaaaag cactagtcac acttgtgaat gaagggcaat tatcagccta
    12901 cggatctggt tctagattcg gtttcatcca tgatttattt ggctttgaac aagcagatga
    12961 tcaaatcgaa gcatccactc acggtcaaag tgtttcttat gaatatgtgc tggaaaaaaa
    13021 tccggatatc ttatttgtag tagacagaac aaaagctatt ggtggagacg acagcaaaga
    13081 tgatatttct gccaatgaac tggtagcaca aacgaatgct ggtaaaaatc agcagatcat
    13141 ctcccttgaa ccagatgtct ggtatttaag cgggggcggt ttagaatcaa tgaaactgat
    13201 gatcgaagat gtgaatcaag catttaaata gcataaataa cagaggatta aactctcata
    13261 ctatgtaaca ataatgtttc ttacattctt aaatagcaag agcctatcct caaagaagtt
    13321 atcaaaaacc atccgtaaaa tcgtattgat gattttacgg atggttttta tttatttgag
    13381 aaatcttttt tcaacgtgaa ttcattatct acatcatacg ggttgattct agaatgggga
    13441 gtggcaaaaa atagtgtgat ccattttttc agcatcaaga tcgcctcctt tgatttgaat
    13501 caaaggtaca aagcgaaact tagataaaac ttaaatttag atatttttat tttgtaccga
    13561 tctcttcttt aaacgatgtt ccatctgctg cttctcggca ataagtccac tagactagaa
    13621 aatatgagaa gctattttct gtccagcgtt cttattactc aaagcaaatc gcagttgtca
    13681 caatctcttc taaacgatgt tccatctgct gcttctcggc aataagccca ctagactaga
    13741 aaatatgaga agctattttc tgtccagcgt tcttattact caaagcaaat cgcagttgtc
    13801 acaatctctt ctaaacaatg ttcaatcagc tgaccttcgg tattagctca aagcactgat
    13861 ttcacaacct ctatttaggc atgtgtctca gcaagcggtt gatagttgat gatctgcatt
    13921 tttcgataaa aatcggcggg aagcttactc caccgtttta ataattgata gaaataactc
    13981 tcttctgctt tgattttata ctcgcttaat atcaatccaa aactattcgt tttttttgaa
    14041 gctaattttt tataaatatc caatgctttg gttttatctt cattgacaaa gagtgttttt
    14101 acttcaatat aagaaccctc gatattttgt gtttcttcga taaaatagat gttttttata
    14161 tattccttca tagtgattcc tccaaagaag tcttttattt gataagttta ctctaccgaa
    14221 gagttgttaa taatccgtga acaaaaagta attctttttc caatagttgt taaaggtttt
    14281 ttatttttta tagtattttt tgttatttaa gaaaggaata aggttaaaca aataacaggt
    14341 tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag aaaagggaaa gttgtctctt
    14401 ttgtgataag agaaatcata ataaacgaat gacttacttt ccaataaatt gagaacagtc
    14461 agaaccagac aaattagttg catctgatga ataaagtgat tattctatga catgaggtga
    14521 tacagatgaa attagaagtg acaaaagaag cacaagaagt attgaagaat aaattagaag
    14581 cagacgatca acttttatta gatatcgaaa acggtgacgg tccatttgca gaaagccaag
    14641 tcagttgcca attggatacg gcatttcgat tgatcattgt caaaaaagat acacaaacag
    14701 atctaagtct ttattctgta gtggctgata caccagttgg accaatcaaa atcaaagaca
    14761 gtgcaatact ttacatggat gatccatcca cacttgcttt agaaccaact tataatagtt
    14821 tacaattaaa aggaccaagc ggcttgagaa aaggcaactt gcaagttgtt cgaaaagact
    14881 agagggataa aatgggaaaa ttggcgatca tcagcgacct acatgtggac atcaataaat
    14941 tcggcgaatc agagctgatg cagctttggc aagttttgca aaagcacaag atcactcggc
    15001 ttcatctagc cggagatacg gctaataaag tagaccgttg cctagctgtt gtcgaatttt
    15061 tcgtcaataa aggattatct acgacatttc atttcggcaa tcacgagttg gcagatgtgc
    15121 ccaatgaaca gatgatggac cattttccag acccacattt tttaaatgaa cggtatattg
    15181 ctttaaatga gcagaccgtt cttttaggga tgaatggctg gtacgactat cagttttccg
    15241 aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt gtattggtat gatcggttca
    15301 tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat gcttgcagct gcaaaaaatt
    15361 tgctagacca gctggaagca aaaaaacttg aggtgatttt atccactcat ttcgttccta
    15421 aacgggcatt tatcatgtat caatctgctc cttatgagcg ttggaacaaa ttaaatgcat
    15481 ttttaggatc agaatcattc ggagaattac ttgaccgtta ttcaaatatc aaacaagtag
    15541 tttttggcca tacgcatcgt cgctttgaag ataaatccat cggtcacacg atttacagct
    15601 gcagaccttt tggttattat tatgaatggc agctgacccg agattttgtc ttgaaagaac
    15661 agttgctaga cagctatgat cctatgaaat tacgtgtcct cttgcgtgcc catcacccag
    15721 cttttcaggc ctatcaaaca caacatttgc aagaggaatt tgaacaagcg atgacaatcg
    15781 ttccgtattg agacagcatt agtaatgtgt aaaagaaaat accttgtaaa acataaggat
    15841 aagtaagagc agtagatcca tttatcgtgg atatgctgct ttttttgcgg aaaataatca
    15901 gcgaatgttt atttgatcgg atgatttgta actcgcagag tactagatgc aagcgtgtat
    15961 tctttttttg aacgataatc acaaaaaaaa atataatata cattttttat ttaattataa
    16021 ttaatattga ttgaagtcta ggcagtcaag tcacattttg atagatagga gggggactta
    16081 cgaattattt taaaggctat gacagctgta tttctagatt gtgtttgcat caagttgcaa
    16141 taaaaactag aaatcaaatc gggctgataa tcatttataa ctaatcgata cataggacca
    16201 aatataggtc tatgttcttt tatttcataa gtatcgtaaa acagaatttt gctatggatt
    16261 aatccaatcg actgatatgt ttagtaaata aagaaagagg aatgtcaatg atgaaaaaag
    16321 aaatcgtatt aacagcagca atgatgttat tcagcatggt agcaagtacg acttttgttt
    16381 ctgcaactga agtataccca aaggaatata acacagaagg aacgattaca tttgaagcag
    16441 gagatgaggg tgtaactcct cctgttgatc cagagaatcc tgatcccaat aaacctgtcg
    16501 atccatcaga tcctccaagc cctggaacag gaggggcatt atcgattgat tatggatcaa
    16561 aattcaaatt tgggactcaa aaaatttcta ctgcggataa aacgtattat gcagctgctg
    16621 atgtgatgaa cgatgggtct agaaaaccga catatgtcca agtgacagat aggagaagta
    16681 ccttatctgg gtggaaatta agtgtctctc aaccagaaca gtttaaaact gccagtgggg
    16741 atgaactagt tggagcacag ctgaaattta cgaaagggca ggctgtttct ttggttgatc
    16801 caacatatac gccgcaaacg gtgaattctg aattaacgtt gacaccggga ggaaacaata
    16861 cgttagcaat taatgctaaa tcaggaacag gtgtggggac ttgggtctat cgatttggtg
    16921 caaatgaaaa tgaaaatcaa gatgcggtac agctttcggt acccggtaaa tctgtcaagt
    16981 tagcgcaaca atattccact aaattagttt ggacattaga agatacacct aacaactagt
    17041 ttactgaaaa gaactgttaa aaataaatac gtagtcatat tgtcttgcga ggtaatcgag
    17101 caaaaattgt caaaggctag gctaaatttg tttggatacg aatagttcat ctaggaattt
    17161 aacgatcagg aggaaaagat atattgaaaa agaggaggat cgtgctcatt ttaagcagtc
    17221 tcttagtgac taatatcttt ccgatcatag ggtactcttt ggaattgaat cagttggagc
    17281 atggaaatag aagagacgaa gaaagcaaac tcacttcgac attcgacagt ttaatagaat
    17341 ccaaccagac acaagaaagc agtgaggatt taagtatttc gagtagtgga gagaccactg
    17401 acaacgtaga agctgagggt tcggacaaac aaggactaga agatgcacca atttctgcgg
    17461 ttgagctgcc tgacgatccc aatattattt ctatcgataa aatttttcaa acacctatcg
    17521 gtactagcac gtccatattg gaaaatggga agttactgca gttgaaccca gcagaaaggt
    17581 ctcaaagagg agctatttgg tcaaataaga agattagttt actctcggat tttacattta
    17641 aatcttattt atacttagga aatcaacgag atagagcggg agatggcatg acctttacat
    17701 tgactggcga tcctagaatg gctagtaatc ccagtcaagt tattggatcg ccaggtatgg
    17761 gaattggctc atattctact cgttctggcc aaccttacat tgcgaatgcg ctctcgattg
    17821 agtttgatac gtataaaaat agtggttctt ctgatcgaat ggatcgagag ataactgctg
    17881 ataatagagg gcatggacat gtcgcttttg tcacgcctaa accaaataat aataattatt
    17941 ctggagagca tactggcgtg acggtagctc ctacatttct ttctaatggc acatggcgct
    18001 tgttaataat taaatgggac gcggagcaaa gaagtcttac ttatgatttg gaaggtgtcg
    18061 gtcactcttc tcggattata cctgatctca ttaggcagtt tggttctaca gaagtaaatt
    18121 gggggtttac ttcttcaaca ggtacttttt ttcaagaaaa tgctgtcgct gtcacgcgac
    18181 ttccatcgaa tgtcacatca acagctgatg taaaagtcaa cgatagtgat tatggggcca
    18241 tcgctgaagc tggaggaaac gataagataa ctatcagaaa tacattgaag gtcgaggggg
    18301 attttcttga atataagaaa atgcagacta gtatcgacct cccacaagaa ttaactattc
    18361 ccgagcatat cttgatcaat ggaaatcaaa ttgatgccaa agatatcttg ataaccggaa
    18421 atcgagtcac gctaaatctg acagattatt ttattgctgg agatacgtta ataatcgaat
    18481 tggagacaac tttgcaggaa gtaattcctg aaaaagtact cactatgcat tttgaatacc
    18541 ttgaaaacgg gaatttgatt gagcaatcga atgaaataaa cctaaagatc gcaaagctga
    18601 aggagataga tatccatgtt tattataaag ataaagacag tcagattgat attgctgaaa
    18661 tgaaaacaat cactgggaaa ataggagaga agtataaaga aactcctatt gatattgaag
    18721 ggtatgtttt tgactcggat tcagcgaata cagaaggcat cttctccgaa aatacagagg
    18781 atatctattt ttattacaga aaaggcaaac tttatctctc aactgctcct caatatgtca
    18841 attttggaaa acataaaata tccagtagac cattaagtga attcggacag ttttcaggtg
    18901 ggttgaaaat tatagatgaa cgggcttttg gaagttggaa attacaattg aaacaagaga
    18961 aagttcttac aaatggagat tttgagcttc cagaagctct ttcctttgtt acctctgaaa
    19021 atacaacggt tatagggaca tccgctgtta cgattttcga aagtgatcaa aagggtgaaa
    19081 gcgatttgtc ctctctttta gattctacag ctcatcgtgg aatcagaata gatattcctg
    19141 tagaaaacca aagaatcgga acctttgagg ggaaactatc ttggatactt gcagatgtcc
    19201 caggaaatta acaggaagag aagaatataa caaaattatt gagtttcaag aagggcacag
    19261 gtaccatagg ctcttgtgtc ccacagagac aagattttgt tatagtcttc tcttacatat
    19321 gattaaagga gcgattttct tgagaccaaa acaacaatta tttataacac tcctagttat
    19381 tttaggggca ctttttaaca atatatcagt gtcagctagt gcatttaatt tttcagtgac
    19441 gccgataact tcagagaatc aaatcgacaa gagaaaaact tacttcgatc ttcaacttga
    19501 tccggatcag gaagtagaag taaaagcgga attgagaaat gatacagaaa aagaagtgaa
    19561 aatcgatatt tctgtgaata gcgcaaccac caattcaaat gtgatggttg aatacggaaa
    19621 aaacgagatt gaaaaagaca tgagcctgat ctttgatctg gtagattatg tgagctatcc
    19681 acagatagtg acgctaaaac ctaaaagtgt ccaaaccgtt gcttttcatg tcaggatgcc
    19741 gaatgagcga tttgatggtg tattggctgg tgggatcaca ttccaagaaa tccagacgga
    19801 aaaggaccaa acagaaacta aggatcaaag cttatcgatt gagaatgaat acacctatat
    19861 tattgctttg ttgatgcaac agacactgaa cgaagttgct ccgaacctta ctcttcatga
    19921 agttaaacca gatcaaatca atgcaagaaa tgtcatctta gcaaacgttc aaaatgatca
    19981 aaaaacctat atcaatcaag tagttattga aacaaaaatt acaaaaaaag gacattctga
    20041 agttttatat caagaggaaa aagaaggatt gcagattgcg ccgaatacaa atttttcttt
    20101 tccaactgct ttaaatggtc aaccgctaac cccaggtgaa tatcacctta caatgactgt
    20161 tttaggaaat gaaaatgaaa atggaaaatt ttcgagaaaa aaaggaaata ctacgatcaa
    20221 ctataccaat caatgggtat ttgaaaaaga ttttacaata gacggaaaag tagccaagga
    20281 attgaacacg acggatgtta cgattaaaaa agacaataat tggatttata tcttagtagc
    20341 ttcacttttc ttattgttta ttttattatg catacttttg tttgttttga gaaaaaaaga
    20401 agagaaagaa aagtaaggtt aagataacaa atagtaaatt tttat
//
LOCUS       DAZKCZ010000040        19996 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000040 DAZKCZ010000000
VERSION     DAZKCZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.040"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG8KN_001375"
     CDS             4..816
                     /locus_tag="ACG8KN_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFP9188441.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG8KN_001376"
     CDS             813..1613
                     /locus_tag="ACG8KN_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9188442.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8KN_001377"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8KN_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFP9188443.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG8KN_001378"
     CDS             3110..4819
                     /locus_tag="ACG8KN_001378"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9188444.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="ACG8KN_001379"
     CDS             5031..9383
                     /locus_tag="ACG8KN_001379"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFP9188445.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="ACG8KN_001380"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="ACG8KN_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9188446.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="ACG8KN_001381"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="ACG8KN_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9188447.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="ACG8KN_001382"
     CDS             11221..11514
                     /locus_tag="ACG8KN_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFP9188448.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="ACG8KN_001383"
     CDS             11511..11822
                     /locus_tag="ACG8KN_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFP9188449.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="ACG8KN_001384"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="ACG8KN_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9188450.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="ACG8KN_001385"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="ACG8KN_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9188451.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..15127)
                     /locus_tag="ACG8KN_001386"
                     /pseudo
     CDS             complement(14623..15127)
                     /locus_tag="ACG8KN_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15292..17655
                     /locus_tag="ACG8KN_001387"
     CDS             15292..17655
                     /locus_tag="ACG8KN_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFP9188452.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="ACG8KN_001388"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="ACG8KN_001388"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9188453.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="ACG8KN_001389"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="ACG8KN_001389"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFP9188454.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6862 a         3513 c         4389 g         5232 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg caactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcattaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgatc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg acagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaaa cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgcct atttgtattg gaaaagccac
    18181 aaaagttatc gagtatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaagaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aagatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtgaaac gattcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggcaaagt tgtcagtatc tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtact
    19021 gggatttttt gacggcgtgc atattggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catcgattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgaggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacagttc atttgcctaa
    19621 agaaggtgtt tatgtatcgg aaataaaaat tggggaaact tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagaat tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcagta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaaaa aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aatttcaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcc
//
LOCUS       DAZKCZ010000041        19616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000041 DAZKCZ010000000
VERSION     DAZKCZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.041"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..3661
                     /locus_tag="ACG8KN_001390"
     CDS             134..3661
                     /locus_tag="ACG8KN_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFP9188455.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3658..7380
                     /gene="addA"
                     /locus_tag="ACG8KN_001391"
     CDS             3658..7380
                     /gene="addA"
                     /locus_tag="ACG8KN_001391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFP9188456.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7449..7790
                     /locus_tag="ACG8KN_001392"
     CDS             7449..7790
                     /locus_tag="ACG8KN_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9188457.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8099..9142
                     /gene="pheS"
                     /locus_tag="ACG8KN_001393"
     CDS             8099..9142
                     /gene="pheS"
                     /locus_tag="ACG8KN_001393"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9188458.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9147..11567
                     /gene="pheT"
                     /locus_tag="ACG8KN_001394"
     CDS             9147..11567
                     /gene="pheT"
                     /locus_tag="ACG8KN_001394"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9188459.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11737..12747)
                     /gene="trpS"
                     /locus_tag="ACG8KN_001395"
     CDS             complement(11737..12747)
                     /gene="trpS"
                     /locus_tag="ACG8KN_001395"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9188460.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13001..13738
                     /locus_tag="ACG8KN_001396"
     CDS             13001..13738
                     /locus_tag="ACG8KN_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9188461.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13752..14567
                     /locus_tag="ACG8KN_001397"
     CDS             13752..14567
                     /locus_tag="ACG8KN_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9188462.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14580..15227
                     /locus_tag="ACG8KN_001398"
     CDS             14580..15227
                     /locus_tag="ACG8KN_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9188463.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15243..15899
                     /locus_tag="ACG8KN_001399"
     CDS             15243..15899
                     /locus_tag="ACG8KN_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9188464.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16061..16882
                     /gene="racE"
                     /locus_tag="ACG8KN_001400"
     CDS             16061..16882
                     /gene="racE"
                     /locus_tag="ACG8KN_001400"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9188465.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16885..18240
                     /gene="rph"
                     /locus_tag="ACG8KN_001401"
     CDS             16885..18240
                     /gene="rph"
                     /locus_tag="ACG8KN_001401"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9188466.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18241..18759
                     /locus_tag="ACG8KN_001402"
     CDS             18241..18759
                     /locus_tag="ACG8KN_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9188467.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18866..19366
                     /gene="cbpB"
                     /locus_tag="ACG8KN_001403"
     CDS             18866..19366
                     /gene="cbpB"
                     /locus_tag="ACG8KN_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFP9188468.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6580 a         3370 c         4208 g         5458 t
ORIGIN      
        1 ttttttgcgt atacttaaaa aagtagaaat atcagaacct taaaaggata taatttgaag
       61 aatatagaac aaatgttcag tattttttga tataataaag gagcaccgta tcatgtgttg
      121 aaggaggaaa agcatgtctt tacaatttat tcttggaaac gggaaaaaag atcaccgtca
      181 agtcttgatc gacgaagcat ccgcttggct cgaccaggat gcaagtaacc aagtattttt
      241 ccttgttccc aactacagca aattcgaaca ggaacaagaa attttagcgg agatgcgaag
      301 aagaagcgga aaaaaaactt tcagcacaac aaacgttcaa gtatttagtt tttatcgtct
      361 ggcttggtat ttgctgcaac agacaactct tcttacagga aatgaattat cagaatcagg
      421 atctgcaatg atcttgcgac agattttaga aaaaaatgag gaagaattaa caatctttcg
      481 cggagaaatc aataaaagag ggtttattcg tcaattacaa gaactctaca gtgaattgcg
      541 tacaggaaat attcaaccag aagacctgat gcttttattt ggtgattcac ctaatgcaga
      601 agaccagcaa cttaaaatga aagacttgaa gctgattttt tcagcttatg acagcgaatt
      661 gactcaacgt gcactaacaa atgaagatgc actatctata ctagcaaatt atatgcagac
      721 acaagatttc aacaacgtca aatttattgt cagtggtttt tctcgaatca atggtcaaga
      781 gtatcaatta ctgcaggcaa tgatggaaaa ggggcaattg gttgtcgatt tattgctgga
      841 tcgtccttat acagcggatc ttccagaagt acttacgctc tttcatgaga ctggaaagct
      901 ttatttccgt ctttttcaaa gtgcaagaga acggaaagtc cctatattag tggaccgatt
      961 tgcaccagag cagactcgtc cgacagagct tcaactgctg caaacaatgt gggaagagac
     1021 atctagccaa aagaaagcgg cagtacacaa aataggggga caagagcatc tccatttgtg
     1081 ggaagctgaa aatccaactg aagaagtccg acaattagct gttgaaatca gacgactagt
     1141 cagtgaagaa aactaccgtt atcgtgatat ccaagtgctg acaaaaaata tgtctttata
     1201 tggaaatatc cttcggccga tctttcaaga aatggcaatt cctttttata tcgatgaaga
     1261 acaaatgatg gaaaatcatc cgctgatcga atggatcaac agtctgttcg ctcttgaccg
     1321 ctattcttat cggctaagtg atattatgcg ttttttcaaa actgaattat ttctgtcgac
     1381 ggaagcagaa accgaacttg atgaatggaa acaagagcga gatgaattca ggaaaaaact
     1441 ggacatcact gaaaatgtag cacttgctta caattatcaa ggaacttatt ggactcgtga
     1501 aaaagactgg cagtttgtgg cgtatgattt tgaagcagaa cagattgaag acgtacagta
     1561 tttggaagaa cagtcaaata cgattagacg attatttcag aaagctgtac cagtattttt
     1621 gaaaaaaata aagaaaagca aaacaggaca agaggccgca aatgtttttt atcaattttt
     1681 gatccaaagt ggcgtacagc aacaattatt gttttggcgt aatcaggaag tagacagagg
     1741 aaacttagaa gcggcaagaa accatgagca gacatgggga gcgctgattg atctgttgga
     1801 tgaatatgta gtaatttatg gtgaatcggc ttttgactgg tcactgttcc aagaaatcat
     1861 tacaagtggt ctagaaaatc taagttatgg taaaatccct accgcaatcg accaagtgcg
     1921 aatcaatcgt ttggaattag ttcgaccaaa tcaagctgcc gtcacttttg cattagggtt
     1981 gaatgatcaa gtgtttccag accgaaaaga gacaaaagga ttgttgtcca gtgaagaacg
     2041 agaagtatta aatcaggcct tgccagatgg acagttttta ttcgacccat caaaggaaag
     2101 tatctctttt gagcctttcc aagcctatct tgtttttctt tccggaacag atcgattata
     2161 tcttagttat gcacaaagct atgacacaga tggttcgctg aagatgtctc cttatttgcg
     2221 tagaatcaca gaatatttat ctgttcccgt cgagagaaaa gagcatttga cgatcgaaag
     2281 cgaccctaat tgttatgtag ggacttacag aagcggaatc aatacagtca ataggattta
     2341 tcgattagct ttagatgaaa aaaaacctgt accaaagcac tggcaaaagc tcaaagaatt
     2401 agtactgaat tcgtcatggc gaaattttgc tttacagatt tttgaaagtc aggaacattt
     2461 aaatgttcct gtttcgatgc cagaaaaaat ggcggaagag ctttatggaa aagagattta
     2521 tacttcaatt tcacgtatgg agaattttta taattgtgaa tacaaatact tcatgcaatt
     2581 tggtttgaaa ttgaaagaac gaactgttta tggtctaact ccagcagcaa ctggtgattt
     2641 ttatcatgaa tcattggatc gtttcttcaa gctactgttt tcaaaccaat tatccttagt
     2701 gaatatgtcc gatgcgcagc gtagagactt tactgaaaaa gtgctgcagg aagtattcgg
     2761 agaacttcgg tttgagatat tggatagttc tgcaagaatg aactatatcc gctaccagtt
     2821 aggacagacg attcaaaaag tgacatgggc tttgcaaaaa cagagcaaaa atacaggcat
     2881 gcatcctctt cagacagaga ttctatttgg tcagattgct ggagcaaaag gtatcacagg
     2941 tctggaatta ccgttgagta atggtggaaa actccatgtt cgagggaaaa tcgatcgaat
     3001 cgatgtagct tctgaacaag aagatacttg gctaagcgtc gttgattaca aatctagcgg
     3061 acgttcattc gatgtcacag aagcttatta tggcatggct atgcaattat tgacgtatct
     3121 ggacgtagca ttgatggatg cggtacgttt gactggaaag gcggctgtta aaccagcagg
     3181 atcatattat ttacatgttc ataatcctgt attgacggag acagatgatg tagagaagaa
     3241 tacattgaaa aaatttagtt atgatggcgt ttttgtcgat gatcccgctc ttttagaagt
     3301 actagatcat tcactggaag caaaagaaca atcactgatc tatccagtga aaaaaaatgc
     3361 gaaagatgaa taccaaaaag gtagtttcag taaagacaaa tttttcacgg aagaagaact
     3421 gcaactattc atggcgcata atcgggaaaa tatgcggtta gctggagatg cgattacttc
     3481 cggagaaatc aaactaaatc cagcttataa agataaacag cggattgcct gcgaattttg
     3541 tccattcaga agcatctgta cgtttgatgt gatgctgaaa gaaaataatt accaccgtct
     3601 ggagaaaata gataaggaag aagcactgcg tagaatattg aaaaggagtg aagaagagtg
     3661 aatcaaattc ctttaaaacc gcaaaatgaa cgattcacag atgatcagtg gcaagcgatt
     3721 tttgatcaag gagataatct attagtctct gcgtctgccg gatcaggtaa aactacagtg
     3781 cttgttcgaa gagtgattga aaaactaaaa atgggttttg atatcgatga attattgatt
     3841 gtgacattta ctgaagctgc cgcaagagaa atgaaagaaa gaattcaaga ggcattacaa
     3901 gaatccgtca atagcgaaag tgaccctgta cgtagacagc attttactaa gcagttagtt
     3961 ttattgccca cagcgaatat ctcaacctta catgcttttt gcctgacagt tattcgacgt
     4021 tattattact taattgatat tgatccagtc tttcgcatgt taacagatga aacagaaacg
     4081 atcttgatga aggaggatgt gtgggacgag ctgagagaag cactttatgc agaaaatgat
     4141 gaacgatttt tccaattaac aatgaatttt tctaatgatc gttcagatga cggcttgacg
     4201 aatctggtgt tttctcttta tgagtttgcc cgtgccaacc ctgaccccca aaaatggcta
     4261 gaacagttaa gcgataatta tcgtttgcct gaggggctag cgaaatctcg attatatcaa
     4321 aaacaaatcc gtcctttagt tttggcagat atataccaat gtgtacagtt atatgaacag
     4381 atgactcagt tagcacaagg tgaaggatta gaaaaaatgc atgagcaagt agctggagaa
     4441 caacagcaga tcaagaatat ctatgaagcg ttctcgcaag atcggttaga agaagcttat
     4501 gctggtttgg aacaactaac tttttcaact tttaagagca gtcggaaagc agaattgaaa
     4561 gaaatttcca atgaagtcaa gggcatgagg gataaagcaa aaaaactgat tcagcagata
     4621 agtaaaagtt attttcctgt ttccccaagc caaatggaag aattaacgga caaagcacta
     4681 ccgttagtcg aagaaatgac aaaagtcacg caaagcttca tggacggttt cagtatgcgt
     4741 aaacgggaaa aaggtgtgct ggatttcaat gatctggaac atttagcgtt gcagattctc
     4801 acagaaaaaa cgaaagatgt atggctgcca agtgaagctt caaaacatta tcgaaaaaaa
     4861 ttcaaagaag taatggtaga tgaatatcaa gacgtgaacc aattacaaga agccatttta
     4921 tactggctaa gagaacccga tgatacaaaa ggaaatatgt tcatggttgg ggatgtcaaa
     4981 caatccatct attctttccg tttagcagat cctagtttat ttatcgggaa atacgagaat
     5041 ttctcaaaga aagaaggcgg acgtcgaatc gttttggctg agaacttccg ctctagaaaa
     5101 gaagtccttt cttttacgaa tcttattttt gaacaactaa tggatccagc agtgggtcaa
     5161 atcaattatg acgaagcagc caaactgatc caaggatttt cagactttcc agagaatgaa
     5221 cagtttgagc cggaaatcat gatctatgaa aaagaacagg aagaatcaga gattgagatt
     5281 ccgacggatg atatactgga agacaaaaca gaaggcgaac tttttatgac aggactaaaa
     5341 attcgtcaat taattgattc ctcctttatg atttatgata aaaaatcaaa aaaaagtcgt
     5401 ccgatagagt acaaagacat tgttctcttg acacctacaa agaagaacaa cttaacgatt
     5461 ttagagattt tcaaaacatt ggatattcca ttagagatga atgatgctca aaactatttt
     5521 caagcgacag aaattcgcac gatgatctcg ctgctacaat taatcgataa tccctatcaa
     5581 gatattccgc ttgcggctgt attgcgctca ccgatcgtgg gtctgattga accagaatta
     5641 gccagcattc gattagctaa ccgtgcacat acttactatg atgctgtgct tgcttatcaa
     5701 gcatcaaacg aagatgaatt agcagctaag ctggaacatt ttggcaagca actagagcat
     5761 tggcgagaac ttgctcgccg ttcttcgatc actgacttgt tatgggatat ctattatgag
     5821 acgggatatc tagagtatgt ggtcggtctg ccagcagggg cacagagaca agccaatctt
     5881 tatgcattgg ttgatcgtgc caaagcttac gagcaaagtt ctttcagagg attataccag
     5941 ttcgtgcgtt tcattgaaaa aatgcaagaa aaagataaag acttagcaga accagtcatt
     6001 tcaatagaag ataatgctgt tcgcgttatg acgatccatg caagtaaagg cttggaattc
     6061 ccagttgtat ttttgttaga tatgacaaag gagttcaact tacaagactt gcgcaatcgg
     6121 tatgcttttg aagaaaaact tggagcagga attcgctata tggatcctga aacacgagtg
     6181 ttatatgata cgttaccgtt tcaagccatc aaattggcaa agcaaaacaa attgttgtcg
     6241 gaagaaatgc gtaaattgta tgtaggattg actcgggcag aacaaaagct tttcattgtc
     6301 ggttcctata aaaataaaga gcaaatgatc caaacgtggt ctgaagcagc tgatcatgag
     6361 gagttagttt ttgatcctgc gctacgtttg aagggcagaa gcagcttgat gaattggatt
     6421 ggttacggat tgatccgtca tcctgaaatg cagaaatact tagaggaaga aatttcgact
     6481 tctcttcttc aacattcaaa tgcacagttt tctatttcat ggatgaatca gcaatcgatc
     6541 attgaacaaa gacagttgct tgctgaaaaa gaactggtta acttagatca gcagatgaaa
     6601 gaagatgaaa ctttattagc agattcgtta caaaaacgtc tggcatatga atatccttat
     6661 caagcttctt cacaaaccac aagttatcaa tctgtatctg aaatcaaacg attattcgaa
     6721 gatccagatg atacacaaga atcacgtttg actctggaaa gcagtcagaa caaagcagca
     6781 tcccgtcaat ttcgttatac gcaagaacaa ctagcagaac caaaattttt gcaaaaagat
     6841 cggcaagtgt cagcagctgc tgttggaact gctacgcatg ctttgttaca attactgcca
     6901 ttagagatgc caactacaga gagtatccat caaaaattac aggaattggt aaggaaacgg
     6961 ttagtagacg aaaaagtcgc gaaaaaagta gatgtttctt ctattatatg gttctttcaa
     7021 acagaacttg gtcaacagct gattgccaac aaagaaaacg taaaacgaga acaacctttt
     7081 tccatgctgc ttcctgctga cgaagtattt caagactatc caaatcaaga agatgaacta
     7141 ttaatccacg gaatcgtcga tggataccta gaagaaaaag atcatctcaa tatctatgat
     7201 tttaaaacag attttatttt acatccggat gatccagcag aaatagatgc aatcgtccaa
     7261 aaatatcaag gacagctaag actttaccaa caagcaatgt cagaagcttt gaataagcca
     7321 gtagaaaatg ttttcctaat tttgcttcga gtgaaacaaa ttataaacat taacaaataa
     7381 ttataattga gcaaacttga cttatgtttt gtaaggatta tgctatactc tgggaagagg
     7441 tgagtatgat ggagctaaca cagacagaat tcgttttatg ccctaaattt gaaaaaacat
     7501 tctctatttt aggaaaaaaa tggaacggat tgattatcga tgtactactc gaaaacggtc
     7561 cgcaacgctt tgttgagctt gcggcaaaaa tcccgatggt cagtgatcga gtattagtcg
     7621 aacgcttgaa ggaattagaa aaagaaggaa tagtcactcg aaactttgcc tgtagcgaaa
     7681 agagccgttc agaatatctc ttgactgaaa aaggagcaga cttgcaaaaa gcgatgcgtc
     7741 aaatacaagt ttggagcgaa aaatggatca cagaggaaga atgcggttga ttttgtaaaa
     7801 aaaatcttgt aaactatagt ccatacgaat aagtaaagaa cgatgacgaa aaagagtagt
     7861 ttggctaaac cagttcaggg agatctgtcg cagactgaaa gcagatcctg tgtttgaaaa
     7921 atgaagttca ctttccgagt tcgtctgata aaatcagacc ggcaattgtc gttaacgaag
     7981 taagagtgcg gtgtaagtca tttatccgca aattaggatg gtaacgcgaa gacctcgttc
     8041 cttaacagga atgaggtctt tttgttttgt atttggaatt taaaggagga acaagcacat
     8101 gtctctacaa gcacaattag agagcttgaa agaagcaacg ctcgtcaaaa tcacagcggc
     8161 acaagattta aatgaattaa atcaaatacg tgttgaaacg ttaggaaaaa aaggaccgat
     8221 cacagaagta ttacgtggga tgaaagatct aagtccagaa gagcgaccaa aagtcggaag
     8281 tttcgcaaat gagatccgag atcttttgac agaagagatc gagcgaaaaa aacaagtatt
     8341 agaagaagaa gcgttaaatg cagctttggc aaaagaaaca atagacgtga cactacctgg
     8401 tagaaaagta aaacatggta cacgtcatat cgtgacacaa atcatggaag aaattgaaga
     8461 tatttttgtg ggtatgggct atcagatcgt agaaggatat gaagtagaat ctgatcatta
     8521 caatttcgaa cggatgaatc tgccaaaaga tcatccagca agggacatgc aagatacttt
     8581 ctatatttca gacgagatat tgattcggac acatacttca ccagtccaag cacggacaat
     8641 ggaaaaacat gatttctcta agggtgctct acgaatgatc tcgccaggga aagtcttccg
     8701 cagagataca gatgatgcga cccacagtca tcagttccat caaattgaag ggctagttgt
     8761 tgataaaaac atcacaatgg gcgaccttaa aggaacatta gaagtcgtaa tgaaaaaaat
     8821 gtttggggaa gatcgtgaaa ttcgtttgcg tccaagttat ttcccattta cggaaccatc
     8881 agtagaagta gatgtcagct gtttcaaatg tggtggtgcc ggttgtaacg tatgtaaata
     8941 cactggatgg atcgaaattt taggagctgg catggtacac ccgaatgtgt tgaagatgtc
     9001 aggaatcgat ccagaagaat attcaggctt tgctttcggc cttggaccag accgcgtagc
     9061 tatgttgcgg tatggagtga atgatatccg taatttctat caaaatgact tacgtttctt
     9121 aagtcagttc aagggggagt aagaaaatgt tagtttcata taaatggctc aatcgttatg
     9181 tagatttatc gaatgtaaca ccaaaagaat tagcggataa gatgtctgtg acaggaatcg
     9241 aagtagaggg agtaacggtt ccagaagaag gcttgaaaaa aatcgttgtc ggtgaagtca
     9301 aggaatgcgt accacatcca gattctgatc acctttctat ttgtcaagtc gatattggcg
     9361 aagacgaact gtctcaaatc gtttgtggag cgcctaatgt gaaggttgga atcaaagtga
     9421 ttgttgcact tcctaactca cgtatcgctg gtaatgtaaa aatcaaaaaa ggaaaaatgc
     9481 gtggccaagt atcaaatggc atgatttgct cattacaaga aattggctat tcagatagtg
     9541 ttataccgaa agaatacgct gaaggtatct attatatgcc acaagaagct gtaaatggcg
     9601 atccagtttt tccttatttg gatatggatg attcgattat tgaattatct atcacaccga
     9661 atcgtgcgga tgcattgtca atgcgtggcg tagcttatga agttggggca atttatcgtc
     9721 agactcctac attcgatgac gaaaagctgg tagaatcctc acgtcctgct agcgataaaa
     9781 ttcaagtgga agttgaagat aaagaagcag ttcctgctta tcaaatcaga atcattgaag
     9841 gagtaagaat ccagccaagt cctcaatggc tgcaaaatct tttgatgaac gaaggcattc
     9901 gtccaatcaa caatgtagta gatgtgacga actatatttt gcttttattc ggacaaccac
     9961 tacatgcatt tgactacgac aaattgaaat caaaagagat ctttgtccgc cgtgcaaaag
    10021 aaaatgaaac attagtaaca ctagatggag aatctcggga attgacaact gacaatctcg
    10081 tcattactaa cggcaaagaa ccagttgctt tagctggtgt catgggaggg ttagactcag
    10141 agatcacgaa tgaaacaaca acagttgcac tagaatctgc tttatttgat ccaatttcga
    10201 ttcgtcgtac agcaaaacaa tttaatttgc gtagtgaatc ctctgcacgt tttgaaaaag
    10261 gaatcaacca tgcgactgtt ggagaagcca gtgaggttgc tgcagcaatg atcgtttctc
    10321 ttgcgggtgg ggaagttcta caaggcgcgg tcaaaggctc agagatcaaa gcagaagacg
    10381 tatcagtgaa gatttcactt gaacgcatca atcaatattt aggaacatct cttgtcgtta
    10441 aagaagtaaa tgagattttt gaagcacttg gatttagcta tgaagaaaag gagtatgttt
    10501 attccgtagt agtaccacca agacgctggg atatccaaat cgaagcagat attatcgaag
    10561 aagttgctcg tatttatgga tacgaccagt tgccatctac acttccaagt ggagaaacgg
    10621 ttgctggaag cttgtcaaaa gaacaagaaa ctactcgtaa aattcgtaca atctttgaag
    10681 gaagcggatt gagcgaagca atcagttatg cattgacaac agaagaaaaa tcaagacagt
    10741 ttgttataaa tgaaagtaat attacacgtt tggactggcc aatgagcgaa gaacgttctg
    10801 tattacgaat gaatttgatc agcggactat tagatgatat cagttataac actgcccgaa
    10861 aaaatacaga gattgcttta tatgaaattg gtcgggtgtt cttccaagaa aatgatccac
    10921 gtacacatct gccgcaagaa gtaaaacatg cagcaatcgc tatttctggt atttgggaag
    10981 aaaaagattg gcagacgaaa gctcaaaatg tagacttttt caccatcaag ggtcttttag
    11041 aaaacttatt tgaacaacta ggcatcactg aagatgtcca ttatcaaaca gtgacagaaa
    11101 tgaaggaaat gcatcctgga agaactgctg cgatttacct tggcgaaaaa tttattggtt
    11161 ttgtcggtca agtgcatcca acgattacaa aaagttatga tattccagaa acatatgttg
    11221 ctgagtttga tttatctgct atagtggaag ctgctcaaaa agggatcgtt ttcgaagcag
    11281 tgacaaaatt cccagcagtc agccgggaca tcgctttgct tgtaaaagaa actgtgacaa
    11341 atcaggaatt aacagaagtt atccgttctg ctgccggaag atttttaaca gatatccaat
    11401 tatttgatgt ctatcaagga gaaaacattg aaaaaggaca taaatccatg gcgtatagcc
    11461 tgacattcgt caacccagaa gcaacattga cagatgaaga aatcaataaa gcaatggaaa
    11521 aagtcgaaaa agcgttggta gaaaacctag aggcaagtat tcgctaagaa ggaaaatata
    11581 gtaagtaacg atcaagtagc ttttgaagac catacatttg aaaaaaagat gtgacacaac
    11641 ttcgtcacat ctcttttttt gattttaaaa aaggaatagc acagagacga gataaaaaaa
    11701 caggccttta cagcctgctt ttttagagta aatgaattaa tggaagtaat tgattcccat
    11761 tgctgatttt acttcagaaa gtgtttgtgc agctacttta gcagcttctt cactaccttt
    11821 acgtaagatt tccataacag ctgaaggatc tttttccaat tcttctcggc gcgcacggat
    11881 cggtgcaaat tcagcttcca atacatcaat gaggtatcgt ttgatcttta catcaccaag
    11941 tccgccgcgg cggtaatgag ctttcatttc ttcaattgct tctttatctg ttccgaatac
    12001 atccaagtag gtgaagacca tattcccttc tacttgtcca ggatcttgta catggatgtg
    12061 gtttggatca gtatacatac tcatgacttt tttctgtaag acatcggcag gatcggagat
    12121 ataaataccg tttcctaacg atttactcat tttgccattt ccatcgattc caggtaatct
    12181 acccattcct tttggtggga atacgccttt tggttccacc aagacttcac catatgtatg
    12241 attgaagctt tgtacgattt cctgtgtctg ctctagcatt ggtttttgat cctcaccaac
    12301 aggtacaaga ttcgctttga atgcagtgat atctgctgct tgagaaacag gatagataaa
    12361 gaatcctgta ggcacgcttt caccgaattt tttttgttcg atttctgttt tgacagtcgg
    12421 attgcgccga acgcgtccta cgctgactaa gttcaaataa tacatggtta attcagctaa
    12481 ttccggaatt tgtgattgga taaataaagt agattttgca ggatctaatc ccacggctaa
    12541 atagtctaaa gcaacttgca agacatttga tgaaaccttc tcaggatttt tagcattatc
    12601 tgtcagtgct tgcatgtcag caatcatgac aaacagttgg ttgtcttcat ctgcctgcat
    12661 agccactcgg gtttttagag aaccgacgta atgccctaaa tgcagcttcc cagttggtcg
    12721 atctcccgtt aaaataatat ttttcaaaaa agacacctct ttacgatttg taagtctgag
    12781 cacttaatat cttaccgttt ttcctgaaaa gaaacaacag ttcctaagga aaaaacaagg
    12841 aaagaggata aaataactaa tagattagaa atggaagaaa tatttatggg agaataaacg
    12901 ttctaagaaa ctttacaaat tactaaacta tcagatataa tgtattttat tatacatctt
    12961 taaaagaaat cttactaaat aataatggga gtgtaattct atggcaatga ttaaatttga
    13021 tcacgtggat aaatattatg gcaagttcca cgcattaaaa aatatcaatc tcgaattcga
    13081 gaaaggagaa gttgtcgttg tcattggtcc ttctggttca ggaaagagca cgatgcttcg
    13141 ctgtatcaat ggattagaga cgatctcttc aggtaagctg ctgattaatg atactgactt
    13201 gcatgataaa aagacaaaac tgacagaagt ccgaaaaaac atcggcatgg ttttccaaca
    13261 tttcaatttg tacccaaata aaactgttct ggaaaacatc aaattagcac caatcaaagt
    13321 gttgaagcaa gaccaggcaa ctgcagtcaa aaacgcggag aaatttttga aaactgtcaa
    13381 tatgttagat aagaaagatt cttatccttc catgttatcc gggggacaac agcaacgggt
    13441 ggcaatcgcc agaggtttag cgatgaatcc tgagatgctg ctgtttgatg aacctacttc
    13501 tgctttggat ccggaaatga ttggtgatgt attagacgtt atgaaaaaat tggctcatga
    13561 cggtatgtca atgattgtcg tgacacatga aatgggcttt gccaaagaag tggcggatcg
    13621 tgttatcttc atggctgatg gagaagtatt ggaagacagc agaaatgtga gagacttttt
    13681 cgaaaatcca aatgaagtcc gagcacagca atttatcagt aaagttatca atcattgata
    13741 ggaggaaaaa gatgcgcagg ttaaaaaaaa tacgcctgat tttcctttta ggcattgctt
    13801 ttcttgcact agccggatgt aaaggaaatg ggttggcaaa tgaagatatt ttagagcgaa
    13861 gcaaatctac caatgaaatc atctggggtg tcaagtatga tactcgatta tttggaatga
    13921 tggatattga gagcagaact gtacaaggat ttgatgtaga tattgcaaaa gcaatcacta
    13981 aaaagatcct tggtgacaat ggaaaaacag aatttgttga agtcacttct aaaactcgga
    14041 ttccattatt gaaaaacgga aatatcgatg caattattgc aacgatgacg atcacagatg
    14101 aacgaaaaaa acaggttgac ttttcagatg tctatttcga tgctggccaa gctttgttgg
    14161 taaagaaagg aagccaaatc aaaagtgtag atgatttgaa tgcttctaca acggttctag
    14221 cagtcaaagg ttctacatcg gctgcgaata tccgacagca tgcaccagat gcaaaaattt
    14281 tagaattaga aaactatgca gaagcattta ctgctttaca atctggtcag ggagacgcga
    14341 tgactacgga taatgcgatt ttgttaggga ttgcagatga aaatccagag tatgagttag
    14401 ttggtggaac gtttactaat gagccttacg ggattgcgat caacaaaggg caagaaaact
    14461 tcttgaaagc tgttaaccaa gcgctagagg aaatgcatgc agatggcaca tacgataaaa
    14521 tctatcaaaa atggtttcca aatgaaactg aaggaaaagt agagtaaggg ggagaggcaa
    14581 tgagcgaatt gatacaaact tatggaccaa tctttgtcga cgggttcaag atcacattgt
    14641 tttcgagtgt cttagcactt gtgtttagtt tgattatcgg aacgttaatg gcaatcttcc
    14701 aattatcaaa gaataaagta gtgagcgcct tagcaaaggc atatgtagaa ttttttagaa
    14761 acattccatt attaatcatc gtgatgtttt tttatgttgt cgtaccgatt tattggttta
    14821 atctagatgg attccaagca ggtacgattg gattaacgat ctatacatct gcttttattg
    14881 ctgaaacagt acgttcaggg attcaaaccg taccaaaagg tcaaacagaa gcaggactat
    14941 cttctggttt tacctattca gaaatcatgc gttatattgt tttaccgcaa gcattcaaaa
    15001 tcgttatccc gccattaggc aaccaattta tcaacctcgt aaaaaactca tctattttag
    15061 caatcgtagc tggtttagat ttgatgtatc aaggagactt gatcgctagt gaaacattca
    15121 ataccttcga tacgtatatc atcgttgggt tattttactt agttatcacg ctaccgttat
    15181 cttacttaat ggtttatctt gagaaaaaat gggcggttcg ttcatagaag gaggggaaat
    15241 acatggattt tagcggcgct ttttcatgga tgaatatccg ttttttattg gaaggattaa
    15301 aggttactgt tgaagtttca ttgttttcga tcatttttag tttcttgatc ggtggtctga
    15361 caggtgtgat tcgttttgcg aatatccctt tcttttccaa aatccttggt gtagtgattg
    15421 atattatacg gaatcttcca ttattattga tcattttctt cacttacttt gccttacctc
    15481 aaatcggcat tcagatgaac atcttctggt ccgctgtcgc agcattaacg atttttgagt
    15541 cagctatgtt gtcggaaatt ttccgagctg gactcaatgc tgttccaaaa ggacagatgg
    15601 aagcaggatt atcaacggga ctgacttatg tagaatcgat gcgaacgatc gtgttaccgc
    15661 aagcatttaa atcaatgatc ccggcaattg tgagccaatt gatttcactg atcaaagata
    15721 cttcattagc agtaatcatt tctttacctg aactttctca tcaggcacgt attatttatg
    15781 gacagaacac caattatgtg cttccgatgt tcatcatcat gacacttatg tacttcgtgg
    15841 tatgttacgc gctttctctg ctttccggtt acttggaaaa gagacggtac agttattaat
    15901 agatgattct tttagcggtt gtggcaaact aaagtcacaa ccgcttttcc tctttccatt
    15961 cgaaaaaaag agatttcgat tgttttaagc aagtaagagc acttgaaaat tcacgctaaa
    16021 aagtacacaa tagggaggat agaatttatt gaaaaaaagg atgatacgat tgacagataa
    16081 tcgccctatc ggatttattg attcaggtgt cggcggcttg actgtagtaa aagaagccct
    16141 gaaacaatta ccgaatgaaa atattttatt tgtaggagac acagcacgct gcccatatgg
    16201 ccctagaccc gcggaacagg taatccagta tacttgggaa atgacggatt atctggtgga
    16261 gcaaggaatc aagatgctgg tgatcgcctg caataccgca actgcggtgg ctttagaaga
    16321 aatcaaagct gctctttcta ttccagtcat cggtgtgatc cttcccggta ctagagcggc
    16381 agtaaaaaaa acacaaaata aacaagttgg cattatcggt acgattggta cggtaaaaag
    16441 tcaagcttat gaaaaagcac tgaaagagaa agtaccagaa ttgactgtga caagtcttgc
    16501 ttgtcccaaa tttgtttcag ttgtcgaaag taatgaatac cattcatcgg tggcgaaaaa
    16561 aattgtggca gaaacattag ctcctttaac cactaaaaaa atcgatacat tgattttggg
    16621 atgcacccat tatccattat tacgccccat cattcaaaat gtaatgggag aaaatgttca
    16681 actgatcgat tctggagcag aaacagtagg tgaagtatct atgctgttag attatttcaa
    16741 tttgagcaat tcaccgcaaa atggtcggac attatgccag ttttatacaa ctggctctgc
    16801 caaacttttc gaggaaatag ctgaagactg gcttggaatc ggacacttaa atgtagaaca
    16861 tatcgaattg ggaggaaaat aaggatgcgt catgacggga gaaaagccca agagcttcgc
    16921 aaactggagc ttcaaacaaa tgttttcaaa tatccagaag gatcggtagt gatccgtttt
    16981 ggtgatacaa cagttatttg ttctgcaaca atagaaaatt ctgttccccc ttttttgaga
    17041 gaaacaggaa caggatgggt gacagcagaa tatagtatgc tgcctcgagc aacgagtacg
    17101 agaaatcgta gagaaagcag caaagggaaa ctttctggta gaacaatgga aatccagcgt
    17161 cttattggtc gttccttgcg tgcagtagta gacttggaaa aattaggaga acgtagtatc
    17221 atcgtagact gcgatgtgat ccaagcggat ggcggtacta gaacagcaag tatcactggg
    17281 gcatttgtgg cattgaaatt agccattgaa aaacttttaa gagaaaaaga attgtcagaa
    17341 gatccaataa aagaacatct tgctgcagtt agcgtaggaa tcttaccaga tgggacttgc
    17401 gtcacagatt tggattacca agaagattcc gcagcacttg tagacatgaa tcttgtaatg
    17461 acagaatctg gaaaattcgt ggagattcaa ggaactggag aagaagcaac gtttgatggt
    17521 gaacagttga atgaaatgct cttttttgga aaaaacgcga ttgaagattt gatcaaagag
    17581 caaaaacatg ctttgctaac agagtttgct caaaatgatg agcggataga agaaacaaaa
    17641 acaatcatca ttgcgacaag aaatccagga aaagcagaag agtttcgaaa tatgttcaaa
    17701 gaagcgggat atcatgtaaa aacattactt gactatcctg aattgccaga tgtggaagaa
    17761 actggcagca catttgaaga aaatgcccga ttaaaagcag aaacgattgc acaattgctt
    17821 gatcagccag tcttagcaga tgattcaggt ttgaaagtgg atgctttagg cggtatgcca
    17881 ggaatctatt ctgcacgttt tgcaggggaa caaaaaagtg atgcggggaa taacgccaaa
    17941 ttattatatg aattgacaga tgtgccggat gaaaaaagaa cagcacaatt ccattgtacg
    18001 ttagtattcg cggcacccaa aaaagacagc cttgtagtag aagcggaatg gcccggtcga
    18061 gtagcccgta taccatctgg cgaaaatggc tttgggtatg atcctttatt tatcccagaa
    18121 ggtaagaaac aaacagcagc agaattatcg agcgaagaaa aaaacaagat cagtcatcgg
    18181 gctcaagcaa tgaaaaaatt gagtgccgag tggaaacaat ggctggaagg agagagatag
    18241 atgcggtatt tagtagtaag tgataatcat ggagacagag atatcctcgt tgacttagta
    18301 gatcgttatc gttctgaagt ggatatcttt ttccattgtg gtgattcgga attagaagct
    18361 tctgacccat tatgggatac attccaagtg gttcaaggta attgtgatta tggaccagga
    18421 ttcgaacaga aaaaagtgat ccaaactggt caggatacag tattcatgac tcacggtcat
    18481 ttatcgaatg tccgatttgg tttgacacag ttagcaatcg aagctcgagc tgcaactgcc
    18541 gatatggtgt tttttggtca tacccatcaa ataggctgtg agatggatca gaaaatcctg
    18601 tttttgaatc ctggtagcat ttctcagcct agaggcccga tccaaatccc atcatatgct
    18661 cttatcgaaa gtttagatga ccaaatttct gttcaatact ataaccgtgc ccatcaggca
    18721 gttgaagagt tggccttttc tttttcgaga gagcgctgaa taaaaaaact ctaattttta
    18781 ataaaaataa gctttatttt atgaattttg catccggttt cggtaaaata gtagtgttga
    18841 atgaaataga aatggaggcc atcagatgat tggacctata gtaagagaaa tgctgctgga
    18901 aaatcaggaa acgtttttga ttcctgcaga aaatgtggca aatgttatgt atcatcatcc
    18961 attgtcccat ggtttacttg tgttatcgaa ggtcggttat tccaaaattc ctgtattagg
    19021 aaaagatgat cactttgtag gactagtaag tttatcagat gtggtcaata aaatgattga
    19081 cttgcaaaca atcagcatgg acccgttaga aggactaacg gttgctgatg tgacagaaac
    19141 agatgttccg acaattgatg aaaattggga actagaagaa gtactccatt tgttagtcga
    19201 ttcgcctttt ttaccggttg tcacagaaga tcggattttc aaaggaatca ttacaagaaa
    19261 agagctgctt aaagccgtaa acttcatggt gcatgaatta gaacgtcgta atatattgtc
    19321 tccgaaaatc gaagaattaa aagaacaaat cgatatcgtg agctaaagaa cgagaaacta
    19381 aaagataatc aaaaaataag tagaaaaaac agcgtaccag ttttgacttt atcttgaaaa
    19441 agaggctggg acaaaagcgt ttaactccaa gaaataagaa ggaattcacg aaaattgctt
    19501 ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt ccgccgttta
    19561 tacgtgttta gagcgtggga caaaagtgtt ctttactttt gtcccacgct cttttt
//
LOCUS       DAZKCZ010000042        19537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000042 DAZKCZ010000000
VERSION     DAZKCZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.042"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..301)
                     /locus_tag="ACG8KN_001404"
     CDS             complement(137..301)
                     /locus_tag="ACG8KN_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188469.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            596..1924
                     /locus_tag="ACG8KN_001405"
     CDS             596..1924
                     /locus_tag="ACG8KN_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFP9188470.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2126..2959
                     /locus_tag="ACG8KN_001406"
     CDS             2126..2959
                     /locus_tag="ACG8KN_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9188471.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2975..3712
                     /locus_tag="ACG8KN_001407"
     CDS             2975..3712
                     /locus_tag="ACG8KN_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFP9188472.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3867..4835)
                     /locus_tag="ACG8KN_001408"
     CDS             complement(3867..4835)
                     /locus_tag="ACG8KN_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9188473.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5067..5900
                     /locus_tag="ACG8KN_001409"
     CDS             5067..5900
                     /locus_tag="ACG8KN_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9188474.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5934..6773
                     /locus_tag="ACG8KN_001410"
     CDS             5934..6773
                     /locus_tag="ACG8KN_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9188475.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6799..7359
                     /locus_tag="ACG8KN_001411"
     CDS             6799..7359
                     /locus_tag="ACG8KN_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9188476.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7558..9279
                     /locus_tag="ACG8KN_001412"
     CDS             7558..9279
                     /locus_tag="ACG8KN_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFP9188477.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9444..10586
                     /locus_tag="ACG8KN_001413"
     CDS             9444..10586
                     /locus_tag="ACG8KN_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9188478.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10602..11813
                     /gene="thiI"
                     /locus_tag="ACG8KN_001414"
     CDS             10602..11813
                     /gene="thiI"
                     /locus_tag="ACG8KN_001414"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9188479.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12386..13033
                     /locus_tag="ACG8KN_001415"
     CDS             12386..13033
                     /locus_tag="ACG8KN_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9188480.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13162..13378
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13426..16071
                     /locus_tag="ACG8KN_001416"
     CDS             13426..16071
                     /locus_tag="ACG8KN_001416"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9188481.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16381..17694
                     /locus_tag="ACG8KN_001417"
     CDS             16381..17694
                     /locus_tag="ACG8KN_001417"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFP9188482.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17684..18337
                     /locus_tag="ACG8KN_001418"
     CDS             17684..18337
                     /locus_tag="ACG8KN_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9188483.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18392..19084
                     /gene="radC"
                     /locus_tag="ACG8KN_001419"
     CDS             18392..19084
                     /gene="radC"
                     /locus_tag="ACG8KN_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9188484.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19234..19434
                     /locus_tag="ACG8KN_001420"
     CDS             19234..19434
                     /locus_tag="ACG8KN_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9188485.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         6584 a         3294 c         4139 g         5520 t
ORIGIN      
        1 aaataaagct tatgcaaaga aaaagaaatc caatcacttg ggtttctttt tcttttatta
       61 ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac gaaagagtag
      121 agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa taaatcagca
      181 ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc ccaaagttat
      241 ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc aggtttttca
      301 ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt attttgaaag
      361 ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc attttcgtta
      421 tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac atttttcttc
      481 atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga gattgtattt
      541 ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg attggatgaa
      601 agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa atatcaaaca
      661 gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg aaaatgtttc
      721 agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga aagtggctaa
      781 tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc gtcataagat
      841 gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt ttttctggga
      901 taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa aagaggcaat
      961 cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg gccaatggga
     1021 tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga tgccggaaag
     1081 tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac tgcgtaaaga
     1141 tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta ttgaagaaaa
     1201 gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag gaacacctcc
     1261 aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg accgaggact
     1321 gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt atgttagcgt
     1381 gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag agatgctagg
     1441 aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa ctttcaaaaa
     1501 attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg atgtaggtca
     1561 atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg atgatctatt
     1621 tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa gtctgatgac
     1681 ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa aatggaaagt
     1741 cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga gtgatgcctg
     1801 gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga aagagcaatt
     1861 aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg gtgctttagc
     1921 atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg cttaagccag
     1981 ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta taattctaaa
     2041 taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac gaagtagaat
     2101 aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg atctgaaatt
     2161 aaatggtaaa gtgattccag ttgttggtgg agatgttaat cagacctatc gtataaaaac
     2221 cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat tttttgaagc
     2281 agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata cgtatatgct
     2341 aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg gaaaagggga
     2401 tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc cacaatttgg
     2461 ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg aagaagattg
     2521 gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag aagaaacgaa
     2581 tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg tattgaaatc
     2641 ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga gtggtaatgt
     2701 gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt atgggaatcg
     2761 ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat ttttagatgc
     2821 ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga tttaccagtt
     2881 atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc aagtagatca
     2941 attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata ccaaaaagca
     3001 tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt cgatgttagg
     3061 agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca gtgaattcgt
     3121 caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta atcgtctccc
     3181 tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata agtttttctg
     3241 ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa tagaatctgt
     3301 tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag agatcattgc
     3361 tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg aaaagattga
     3421 ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta atcagcatgt
     3481 taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact ttttgactac
     3541 gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg ggaatgagat
     3601 cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac atgaaatccg
     3661 aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat aaaaaagagg
     3721 ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat tgtttctcaa
     3781 atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag cctctctttt
     3841 taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa ttcttcaccc
     3901 gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact attaatactg
     3961 ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata atcataaaca
     4021 ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt ttggacaaga
     4081 agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa agcttcgatt
     4141 accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg catcccagca
     4201 tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg ctttgtttct
     4261 aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg acccaatgta
     4321 ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg tgcagaatgg
     4381 atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca acttgcgaca
     4441 cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact tgaacgcatt
     4501 gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa aaaagcagtt
     4561 tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga tgcataagcg
     4621 gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg tgagggaaga
     4681 ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc tgcatctaat
     4741 aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt catggcgatc
     4801 gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt acgaataatt
     4861 ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat cagtgaagaa
     4921 taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa aagactcaac
     4981 cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa agcagccaag
     5041 gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg tttaaagaac
     5101 atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg tttctaccct
     5161 tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt gagaattgga
     5221 taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga atcagcggtc
     5281 aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat tttttagatg
     5341 ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa ttcccctcat
     5401 cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag tggtttgagg
     5461 gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa tttcttgaac
     5521 gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat gagccacagt
     5581 tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt gctctttttc
     5641 gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg cgtaaaaaaa
     5701 gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa gtcaaagtag
     5761 ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt ggtgatgcgg
     5821 ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga ctcaatccaa
     5881 cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag tctatgatca
     5941 aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa gcgttggaat
     6001 caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt gtagcaacgg
     6061 ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat atgtatgtcg
     6121 tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac ccttttgaac
     6181 aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa atcgtttttg
     6241 gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg atttttatca
     6301 agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa aaaatcctcc
     6361 aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata ttaaaagaac
     6421 caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta gaacatcgct
     6481 ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc ccaaaaggtg
     6541 gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa atggaagaaa
     6601 ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta ggaattggag
     6661 tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg actcaatcaa
     6721 atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat tagtcaatga
     6781 aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga aacattccac
     6841 aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa tgaagctcat
     6901 acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac ttcacctaat
     6961 aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa agcagaagaa
     7021 aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt agatgcactg
     7081 acagatatcc tttactttac ttatggttct ttttcattac taggaatagt gcctgatcga
     7141 atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg aaaacctcat
     7201 tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga atatgctcca
     7261 gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc taagaagaaa
     7321 tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca ctatttttgt
     7381 tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg catgaaaata
     7441 gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt ggatttatgc
     7501 caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga accataaatg
     7561 ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat tgtcgtcttt
     7621 ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt agaaaaacgc
     7681 aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa aaaaatgcat
     7741 ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa tgacatatct
     7801 acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct caatgaaagt
     7861 tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat ggaccaaatg
     7921 gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac ggaagaacgc
     7981 aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga aaaagtcaga
     8041 gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat caaagtgatc
     8101 gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt tgaagcccgc
     8161 gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat gaaaaaaatt
     8221 ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga aatcagtgat
     8281 acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct tgctgaagat
     8341 atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa aacagaagtt
     8401 gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt atatgatatc
     8461 ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac gattgcagag
     8521 tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga tcacacatct
     8581 caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca aaccgaagtg
     8641 gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca cgaaattcct
     8701 tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga tatcgaaaag
     8761 caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa agaagcgcaa
     8821 gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt agaaaaacaa
     8881 cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac tgaccgtatt
     8941 gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt caatcgttta
     9001 gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga tttagtagat
     9061 gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca tacacacgaa
     9121 gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata tcactatcaa
     9181 gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc tttcaaacgc
     9241 atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt tagtaaacca
     9301 gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt gaaaattgaa
     9361 acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata atagaaaaga
     9421 atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca accccaatct
     9481 ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg aatccatcaa
     9541 gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga aagcagatcg
     9601 ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga acagaaggag
     9661 ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat catatgatca
     9721 tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa gaattaggaa
     9781 tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac gaattgaaag
     9841 aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat gaagtaggat
     9901 cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa attcatttcc
     9961 atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg acagaaagag
    10021 ttgattttgc aacattttca gctcataaat tccatggccc acggggaact ggtttcattt
    10081 attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa gaaagaaatc
    10141 agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct ttacgtttat
    10201 cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta gctctaatca
    10261 atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac tatgcaccgc
    10321 atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat gccttagaag
    10381 aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat atggcaagca
    10441 gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt cgaatcagct
    10501 tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc aagcagcttt
    10561 ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac agtgaaatta
    10621 tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt atcatgcaac
    10681 tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc catgctgatc
    10741 gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt ccaaaactag
    10801 caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa gatgtcgaag
    10861 ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa gaaacattta
    10921 aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa ttgaatcaaa
    10981 cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg cgtcaaccag
    11041 atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat gaaacgatca
    11101 agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg ctttctgggg
    11161 ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa attgaagctg
    11221 ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca aaagatctaa
    11281 cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca tttaccgaga
    11341 ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt actcgtcgaa
    11401 tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc attttgaatg
    11461 gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct atcaatgaag
    11521 taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa atcattgaag
    11581 tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat tgctgtacga
    11641 tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat caatatgaag
    11701 agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag atggaagaga
    11761 tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta taagaggtta
    11821 tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag ctccatgtat
    11881 tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta tcaagaagtt
    11941 atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag agatcaagct
    12001 acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt tgtgacagaa
    12061 gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa tttttgtgaa
    12121 atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga ggttgcgaaa
    12181 aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttacg
    12241 tttggttgag cttattaccg cagatgcaag cttttgaaca ccgttttatt tgtctcacac
    12301 ccttttcctt cctacttgta accttgctcc cgtgttagaa acatgttaca ctaaatatgt
    12361 gaaattatta acaaggagcg tcgttatgcg agaaaaaaca gatacactgc caaaagcaac
    12421 tgcgaaaaga ttacctttat atttacgcta cttgaagatg ctggatgact caggtataag
    12481 tagaatcaaa tcaaatgagt tcagcgaaat cacccaaatt ccttctgcaa cgatccgcag
    12541 agacttctcg caattaggcg agctaggcag aagcggatat ggctatgatg tcccttttct
    12601 tatcgatgta ttcaataata tccttaatac taaagaagaa aaacgaatcg cattagtagg
    12661 atatggaaat cttggcaaag cattgaaaca caacaatttt agaagaaatg aaaatctaaa
    12721 tatcgtgtgc gtctttgata atgatccagc actgatcaat cgagtaattg atggagaaat
    12781 gatctatccg attgatcgtt tcgcggagat agccaaagca aaaaatgtga cagtagcaat
    12841 ctctacggta ccaagtaaat attcacaatc tgctattgat gaaattgtca aaggaaatgt
    12901 aaccgctata cttaattttg cgcctgacag agtaactgtc ccagcttatg tcaatgttca
    12961 atacattgat ttgacaaccg agttgcagac gcttatttac tttgatgaga attactcaga
    13021 agttttttct taggaaaatt tgagtaggct tattgatctt gcgagaactc gtcgtaattc
    13081 tttcaaaaat tttttagtac taaatgaagt ggttttttca cgaccacttg ttgactaaaa
    13141 aaaaaaacca ttgtagaatg aagcataacg ctgaacaaga ggagtaactg gcacattcat
    13201 tcttagagag agaactatct gctgaaaggt tctcaatgac aaccagtgaa ggtagcttgg
    13261 gagtttctgt cttgaatgga gtagagatag acgagtcgtg atcgttaact cacttgagag
    13321 gcaataatga ttgattattg caatttaggt ggtaccgcgt aaatcatacg tcctagaata
    13381 cgaaagtatt ttaggacgtt ttttagttta aaggaggaaa acagcatgac ggaagaaaat
    13441 aacctatcaa caaaatataa tccgcaagaa gttgaagctg gacgttatca gcattggtta
    13501 gaacaagatt tgtttaaacc gagcggagat aaaaaagcaa aaccttattc aatcgtaatc
    13561 ccgcccccaa atgttactgg aaaacttcat ttagggcatg cttgggatac gaccttgcaa
    13621 gatatgctga ttcgtcaaaa gagaatgcaa ggatatgata ctttatggct gcctggtatg
    13681 gaccatgcag gaatcgcgac acaagcaaaa gtagaagcga agcttagaga gcaagggatt
    13741 tctcgttatg atctaggtcg tgaaaagttc atcgatcaag tgtgggaatg gaaagaagag
    13801 tatgcgagcc acatccgcga acagtgggct aaaatgggct tatctttaga ttacagtagg
    13861 gaacgtttta cattagatga tggattatct gaagcagtac gtaaagtttt tgttacctta
    13921 tacgaaaaag gtttgattta tcgtggtgaa tatatcatca actgggatcc tcaagcacga
    13981 acagctctat ctgatatcga agttattcat aaagatatcg aaggcgcctt ttatcatatg
    14041 agctatgaac tagcagatgg atcaggcgta gtagaaattg cgacaacccg tccagaaacg
    14101 atgttgggag atacggcgat tgctgttcat ccagaagacg agcgttatca agcgctcatc
    14161 ggcaaaaaag tgattctacc tttagtcaat aaacaaatcc caatcattgc agatgaatat
    14221 gtagatatgg aattcgggac aggtgtagta aaaatcactc cagcacatga tccaaacgac
    14281 tttgaggtag gtaatcgtca tgatcttcca cgcgtaaatg ttatggatga tgatggtacg
    14341 atgaatgaac tagcaggaaa atatgctgga atggaacgtt ttgctgctcg taaagcaatc
    14401 gtgcaagatc tgaaagaaat cggtcgattg attaaaatcg atacgatgat ccacagtgtg
    14461 ggtcattccg agcggacagg tgtcgttgtt gaacctcggc tgtctactca atggtttgta
    14521 aaaatggcgc cgttagcgaa aaaagccatt gataaccaag aaacagatca agcagtggaa
    14581 ttcttccctc caagattcaa tcaaacgttt ttacgttgga tggaaaatgt ccatgattgg
    14641 gtaatctcac gacaattatg gtggggacat caaattcctg catggtatca caaagaaaca
    14701 ggtgaaatgt atgtagggat ggaagcacca gcggatagtg aaaactgggt acaagatcct
    14761 gatgttttag acacttggtt tagttcagct ttgtggcctt tctcaacaat gggctggcca
    14821 gatacggaaa gcgaagatta taagcgatac ttccctacta gcactttagt aaccggttat
    14881 gacatcattt tcttctgggt aagcagaatg attttccaaa gcttggaatt tactggcgaa
    14941 cgtccatttg agaatgtctt gattcatgga ttgattcgtg acgaacaagg gcggaagatg
    15001 agtaaatcat taggtaatgg aatcgatccg atggaagtaa tcgaaaagta tggagcagat
    15061 gctctccgct ggttcttatc gaatggttct gcaccaggac aagatgtccg cttcagctat
    15121 gaaaaaatgg atgcttcttg gaactttatc aacaaaatct ggaatgcttc acgtttcgtt
    15181 atcatgaata tcgaaggaat gaccgtagaa gagatcgatt tatctggaga aaaatcagtg
    15241 gctgaccggt ggatccttac tcgtttgaat gaaacgatcg aacgagtaac tgagctattt
    15301 gatcgttttg aatttggtga agcaggtcga caactgtata actttatctg ggatgatttt
    15361 tgtgactggt acatcgaaat gagtaaagaa gtactttatg gagatgatgc tgtttcaaaa
    15421 caaaccaccc gtagcatctt agtccataca cttgatcaga tcctacgttt attgcatcca
    15481 atcatgccat tcgttacaga agaaatatgg gaacatattc ctcatcaggg aaattctcta
    15541 gtagtggcag aatatcctgt tgtacgtcca gagtttgatg atgaaacagc ttctaaaggg
    15601 atggaagtat tgaaagaatt gattcgagcc gttcgaaata ttcgttcaga agtgaataca
    15661 ccattgtcta aaccaattac gttgctaatt aaaacaaacg atgctaaaat cgatcaattt
    15721 ttagttgaaa atacaagcta tatcgaacgc ttctgtaatc ccgaagagtt gacgatatca
    15781 agtgagatca ctgcgccaga tctagcgatg tcagctgttc tgacaggtgc agaaatcttc
    15841 ttgccgttag caggattgat caatattgaa gaagaaatca aacgattaga aaaagaactg
    15901 gaaaagtgga caagcgaagt aaaacgtgtc caaggaaaac tttctaatga acgttttgtt
    15961 tctaacgcgc cggaagaagt agtagaggca gagcgtgcaa aagaaaaaga ttatttagag
    16021 aagcaaacgg ctgtcaaaga acgaatcgaa agtttgcgta ctattcaata agaatagaaa
    16081 aagagagtgt gaaatcagct agctattact aaaagctagc tgattgaaca tcgttacagg
    16141 aaagagtttg agcctcactc ttagcttcga ttcataagga gatgtgacat aaattttgtc
    16201 gcatctcttt tttagtctac tcttattatg taggagaagg agggaggtgg atcatgtatc
    16261 aagagtcaat aaagtagttg ataaaatcgt tcctctctct gtttcttttc tctttgtcat
    16321 acttttattt ttcttatgtg ttctatttgt tataataaaa gagaaatggg ggaattgttg
    16381 atgacgattg aagaagcaat agaatggatc catagtcgat taccatttgg ttcacgtcct
    16441 ggccttgacc gagtcgaagc tttattagaa gtggtagggc atccagagag aaaagtaaaa
    16501 acgatccata tcgctggaac aaacggaaaa ggttctactg tagcttattt gagaagccta
    16561 ttagaagagg ctgggctgac agtaggtaca tttacttcac cttatatcga aacgttcaat
    16621 gaacggatcg caatcaatgg caaaagcatt ccagacaatg atttgatcca gcttgtaaaa
    16681 aaatatcagc cactgatcaa acaacttgat caatttgaag cagtagctgg tattactgaa
    16741 ttcgagacat taacagcaat ggcatttgat tattttttga tgaagaaagt ggacgtagcg
    16801 attatcgaag ttggcttagg cggcttattg gattctacca atgtagctgt accaatgctg
    16861 acgggaataa caacgattgg acttgatcat accgaaatac ttggagatac aattgaagaa
    16921 atcgctgcac aaaaagctgg aatcatcaaa caaggcgttc cagtagtaac aggaaatatt
    16981 gccccagaag cttttactgt catccaacaa acagctaaag caaaaaatgc accgctttat
    17041 gtatacgggg aaaattataa aataaactat ctgcatcctg atgagatgtg gggagaagta
    17101 tttgaatttt ttaatgaaat aggaaagatc cctcaattaa ccactccgct tttagggcaa
    17161 catcaagctg aaaatgcagg agtagccatc gaattgtatt ttgtttactg tcaattggaa
    17221 aagctgcctt tccgagaaaa agatgttttg aagggattga agaaatctcg atggccagga
    17281 agaatggagc gtttgagtaa cgagccatta gttgttttag atggtgccca taatacgcat
    17341 gcaatgaacc ggcttgttga gaatttgaaa aaagaattca aaggatatca aatcaacatt
    17401 ttgttttccg ccttacgaac taaaaatgtt agagaaatgc tagaacgatt gcagcaagta
    17461 ccaaatgtcc atatttattt gacgaccttt gaacatccaa aagccatcga attaagtgaa
    17521 gtatcagatt tagcaagtga gagagtatca atcgtttcat tgtggcaatt cggattagca
    17581 gatatattgg aaaagatgac aaatgaagac gtattagttg tgaccggttc actttatttc
    17641 atttcagaag ttcgccattt attaatacaa ttaggaggag atcatgaagt ataatgcagt
    17701 gatttttgac atggatggtt tgctgttcga cacagagtta gtgtattacg aagccacaca
    17761 agtagtagca gacaagatgg gttttcctta tgataaagac ctgtatttaa aattcttagg
    17821 tgtttcagat gaagaagtat gggcgaatta tcatatgatt ttctcggaat ttggcaaaac
    17881 aagtgtacaa agattcatag atgattcata ccaggaaacg cttgaacgtt tttcgcaagg
    17941 agcggtacaa atgaagcccg gtgtgttgga actcctctct tttttagaag aacatcagat
    18001 tcccaaagtt gtcgcttcca gtaaccaacg agcagtcatt gaacttttat tgaagaagaa
    18061 caatctgttt gaacgatttg agacgattgt ttctgcagaa aatgttcaac gagcaaaacc
    18121 agacccagaa attttcttgt tagctaatca gaagctaggt acagcgaaga aagaaacatt
    18181 gattttagaa gattcacaaa atggtatttt agcagcttct gcggctgaga tcccggtagt
    18241 catggtacca gatctgcttc caccatccca agaattgatc aaaaaaacag ctggggttgt
    18301 ttcttcttta catgaaatcc ctgcattgtt aaaataaaaa tccatctctt ttcgtatctt
    18361 attttagata agatagaaag gagatgtttt tttgggaaaa cgattgatca aagaagtacc
    18421 tactagctct ttaccaaggg aacgtatgga aatttatggc gcggaagctt tatcggatca
    18481 ggagcttttg gcaatcttac tgcgtaccgg tcaacaccct tatagtgtga tgtcgattgc
    18541 tggaaatctg ttaaaaacgt ttggaggact ggcctcttta cgacaggcta ctttgcatga
    18601 gttagaagag attcgaggga taggacgcgt gaaagcaatc gaaatcaaag ccttgatcga
    18661 attaggtcga cgaattcaga cggatcatga aagagcggct ccaccggttc gtaccagcta
    18721 cgaattggcc cagcaattga ttctggaaat gaaagatcac aaacaagaac atttggtttg
    18781 tatttatttg gacacgaaga atcaagtgat cttaaaaaaa acagtgttca tcggttcgct
    18841 gaatcagtca gtcgctcatc ctagagaaat tttccattat gctgtacgtt attgtgcagc
    18901 aaggatcgtt ttagcgcata atcatccttc tggtaatgtt attccatcgc agcaggatat
    18961 gaattttacc aaaaggatac aaaagtgtgg agaaatgatg gggatcactg tactggacca
    19021 cttgatcatc ggacgcaaac gatattttag tttgagagaa gaaggaatga tggaagagaa
    19081 gtaagttgct ttcttgcaaa aaaaatgaag caagtgtaca attatattgt aattttgttt
    19141 gggaatagaa gttgaaacat ctgttgcacc tctatcgtac acgagcaatg ttacaattta
    19201 aattagtgct tttggcacga ggaggatatt attatggaaa acggaacagt aaaatggttc
    19261 aacgctgaaa aaggatttgg atttatctca cgtgaagatg gaagcgacgt attcgttcac
    19321 ttctcagcta tccaaggtga cggattcaaa acattagaag aaggtcaagc agtgactttc
    19381 gacgttgaag aatcagaccg cggcccacaa gctgcaaacg ttgtaaaagc ttaattttat
    19441 ctgtaaatca aattgatggt agacatcaat ttttacgaga gaacaaagcg agtaacaaaa
    19501 atacctgcca caagagacaa tttctcttgt ggcaggt
//
LOCUS       DAZKCZ010000043        19403 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000043 DAZKCZ010000000
VERSION     DAZKCZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.043"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1195
                     /gene="pgk"
                     /locus_tag="ACG8KN_001421"
     CDS             5..1195
                     /gene="pgk"
                     /locus_tag="ACG8KN_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9188486.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            1299..2054
                     /gene="tpiA"
                     /locus_tag="ACG8KN_001422"
     CDS             1299..2054
                     /gene="tpiA"
                     /locus_tag="ACG8KN_001422"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9188487.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            2170..3468
                     /gene="eno"
                     /locus_tag="ACG8KN_001423"
     CDS             2170..3468
                     /gene="eno"
                     /locus_tag="ACG8KN_001423"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9188488.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            3867..5006
                     /locus_tag="ACG8KN_001424"
     CDS             3867..5006
                     /locus_tag="ACG8KN_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFP9188489.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(5073..5648)
                     /locus_tag="ACG8KN_001425"
     CDS             complement(5073..5648)
                     /locus_tag="ACG8KN_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFP9188490.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(5653..6627)
                     /locus_tag="ACG8KN_001426"
     CDS             complement(5653..6627)
                     /locus_tag="ACG8KN_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFP9188491.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(6651..6695)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            6910..7878
                     /locus_tag="ACG8KN_001427"
     CDS             6910..7878
                     /locus_tag="ACG8KN_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9188492.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            7975..8268
                     /locus_tag="ACG8KN_001428"
     CDS             7975..8268
                     /locus_tag="ACG8KN_001428"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9188493.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            8386..8622
                     /gene="secG"
                     /locus_tag="ACG8KN_001429"
     CDS             8386..8622
                     /gene="secG"
                     /locus_tag="ACG8KN_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9188494.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            8725..9480
                     /locus_tag="ACG8KN_001430"
     CDS             8725..9480
                     /locus_tag="ACG8KN_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9188495.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            9483..11840
                     /gene="rnr"
                     /locus_tag="ACG8KN_001431"
     CDS             9483..11840
                     /gene="rnr"
                     /locus_tag="ACG8KN_001431"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9188496.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            11862..12326
                     /gene="smpB"
                     /locus_tag="ACG8KN_001432"
     CDS             11862..12326
                     /gene="smpB"
                     /locus_tag="ACG8KN_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9188497.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            12628..13347
                     /gene="atpB"
                     /locus_tag="ACG8KN_001433"
     CDS             12628..13347
                     /gene="atpB"
                     /locus_tag="ACG8KN_001433"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9188498.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            13400..13615
                     /gene="atpE"
                     /locus_tag="ACG8KN_001434"
     CDS             13400..13615
                     /gene="atpE"
                     /locus_tag="ACG8KN_001434"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFP9188499.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            13706..14230
                     /gene="atpF"
                     /locus_tag="ACG8KN_001435"
     CDS             13706..14230
                     /gene="atpF"
                     /locus_tag="ACG8KN_001435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9188500.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            14217..14759
                     /locus_tag="ACG8KN_001436"
     CDS             14217..14759
                     /locus_tag="ACG8KN_001436"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9188501.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            14782..16338
                     /gene="atpA"
                     /locus_tag="ACG8KN_001437"
     CDS             14782..16338
                     /gene="atpA"
                     /locus_tag="ACG8KN_001437"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9188502.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            16352..17254
                     /locus_tag="ACG8KN_001438"
     CDS             16352..17254
                     /locus_tag="ACG8KN_001438"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9188503.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            17277..18683
                     /gene="atpD"
                     /locus_tag="ACG8KN_001439"
     CDS             17277..18683
                     /gene="atpD"
                     /locus_tag="ACG8KN_001439"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9188504.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            18697..19119
                     /locus_tag="ACG8KN_001440"
     CDS             18697..19119
                     /locus_tag="ACG8KN_001440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9188505.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         6505 a         3517 c         4153 g         5228 t
ORIGIN      
        1 taaaatggct aagaaaacag tgaaagatat cgatttaaaa gacaaaaaag ttcttgtccg
       61 tgttgacttc aacgttcctt tgaaagacgg cgtgatcact gacgacactc gtatcaaagc
      121 agcattgcca acaatcaact acgttctaga acaaggcgga aaagcaatcc ttttctctca
      181 ccttggacgt gtaaaaacag aagaagataa agaaggaaaa tctttagctc cagttgcaaa
      241 acgtctaggc gaattattag gtaaagaagt aacattcgta cctgaaactc gcggagaaca
      301 attagaagaa gcaatccgca acatgaatga cggcgacgtt gtcgtattcg aaaatacacg
      361 ttttgaagat attgacggta aaaaagaaag caaaaatgat acagaactag gtaaatactg
      421 ggcttcatta ggcgatgtat ttgttaacga tgctttcggt acagctcacc gtgcacatgc
      481 ttctaacgta ggtatcgctt caactggtat cccaacagtt gctggattct tgatggaaaa
      541 agaaatcaag ttcatcggtg aagcagtaga agaaccaaaa cgcccaatga ttgcaatcct
      601 tggtggcgca aaagtttctg ataaaatcgg cgtaatcgaa aacttgattc ctaaagcaga
      661 taaaatctta atcggtggcg gaatgactta cacattctac gaagcaaaag gaatcaagat
      721 cggtaactct ctagtagaag ctgataaagt agaactagca aaagaattaa tcgaaaaagc
      781 aggcgacaaa cttgtcttgc caatcgacaa tgtctgtgca ccagaatttt caaatgatgt
      841 tgaaacacaa gtaatcgaag gagacattcc tgatggtttg atggctttag acatcggccc
      901 agcatcagta aaattatttg ctgatacatt acaaggtgca aaaacagttg tttggaacgg
      961 acctatgggt gtatttgaaa tgagtaactt tgctaaaggt acaatcggtg tttgtgaagc
     1021 aatcgctaac ttagaaggcg caacaacaat catcggtggc ggagactcag ctgcagcagc
     1081 agaacaatta ggattcgctg acaaattcac acacatctca acaggtggtg gcgcaagctt
     1141 ggaattgctt gaaggtaaag aattaccagg actagcagca atcaacgata aataaacgac
     1201 caagtttgct caattctgac gagtaatcga tcaaaaggtg aagggagttt tcatgactcc
     1261 ctagccattt ttcaataaat taaggagtgt gttcctcaat gcgtaaacct attatcgcag
     1321 gtaactggaa aatgaacaaa actgcatcag aagcaaaaga atttgctgaa gcagtaaaaa
     1381 acaaaatccc ttcaaatgac aaagttgatt cagtcatcgg atctcctgca ttattcttac
     1441 aagaattaat gacatcagct gaaggtacag aactaaaaat ctcagcacaa aactgctact
     1501 gggaaaactc aggtgcgttc actggtgaaa cttcaccagc agctcttgct gatctaggtg
     1561 ttcattatgt gatcatcgga cattcagaac gtcgtgaata tttccatgaa acagacgaag
     1621 acatcaataa aaaagcaaaa gctatctttg caaatggtat gacaccaatc ctatgctgcg
     1681 gcgaatcttt agaaacctat gaagcaggca aaactgctga atggatcgaa ggacaaatta
     1741 cagctggttt agctgatctt tcagctgaac aagtgtcaaa catggttatt gcttatgaac
     1801 caatctgggc aatcggtact ggtaaatcag ctgacgcaca aatcgctgac gaaatttgtg
     1861 gagttgtacg tcaaacagtt gaaaaacttt atggaaaaga agtatcagaa gcagttcgta
     1921 tccaatacgg cggttctgta aaaccagaaa atattgctga atacatggca aaagaaaatg
     1981 ttgacggtgc tttagtcggt ggcgcaagct tgcaagcaga ttcattcttg tcattgttag
     2041 acgctgtaaa ataattgttg aaaatttgtg aaaaatgcaa gaaatgtttg cactaatgag
     2101 caaaattaac taaaatcgta tatgagggaa atcccttagg aaaaaacaaa ctcaaaggag
     2161 agacaaaaca tgtcaattat tactgatgtt tacgctcgcg agatcttaga ctcacgtggt
     2221 aacccaacaa tcgaagtaga agtatacaca gaaagcggag cttttggccg cggaatggtt
     2281 ccttcaggtg cttcaactgg tgaatacgaa gcagttgaat tacgtgacgg agacaaagct
     2341 cgttatggcg gtaaaggtgt aactaaagct gtcgacaacg taaacaacat cattgctgaa
     2401 gcaatcattg gctacgatgt tcgtgaccaa atggctatcg acaaagctat gatcgcttta
     2461 gacggaactc ctaacaaagg taaattaggt gcgaacgcta ttttaggtgt ttctatcgct
     2521 gttgcacgtg cagctgctga ctacctagaa gtacctttat accactacct aggcggattc
     2581 aacacaaaag tattgccaac accaatgatg aacatcatca acggcggatc acatgctgat
     2641 aactcaatcg acttccaaga attcatgatc atgcctgttg gcgctccaac attcaaagaa
     2701 gctctacgta tgggtgctga agtattccac gcattagctt caatcttgaa agctcgcgga
     2761 ttagcaactt ctgttggtga cgaaggtgga tttgctccaa accttggttc aaacgaagaa
     2821 ggttttgaag taatcatcga agcaatcgaa aaagctggct atgtacctgg taaagacgtt
     2881 gttcttgcta tggatgctgc ttcttcagaa ttctacgaca aagaaaaagg tgtttacgta
     2941 ctagctgact caggcgaagg cgaaaaaaca actgacgaaa tgatcaaatt ctacgaagaa
     3001 ttagtttcta aatatccaat catctcaatc gaagatggat tagacgaaaa cgactgggac
     3061 ggattcaaga aattaactga cgtattaggc gacaaagttc aattagttgg tgacgacttg
     3121 ttcgtaacaa acactcaaaa attatctgaa ggtatcgaaa aaggaattgc taactcaatc
     3181 cttatcaaag tgaaccaaat cggtacatta acagaaactt tcgaagctat cgaaatggct
     3241 aaagaagctg gctacacagc agttgtatct caccgttctg gtgaaacaga agattcaaca
     3301 atctctgata tcgctgttgc aacaaacgct ggccaaatca aaactggttc tctatcacgt
     3361 actgaccgta ttgctaaata caaccaatta ctacgtatcg aagaccaact tggtgaagtt
     3421 gcagaataca aaggtttgaa atctttctac aacttaaaaa acaaataatt cttttctaat
     3481 gaagatacat gatcgactat tgaaagatag tcgagtatga tcacttagtt aagtaatcga
     3541 actctctact tgctgaaata tcagcaggta gagagttttt ttgcaactag cttctgtata
     3601 tgatgtttac aaagaatggt acaagaaaag atcatccttg aaaaaggaaa gctaacaata
     3661 actagcatca tataagagct tttaaggata gaacagtaga ataggaatct atgagcaagt
     3721 cctctcgtat aaataaaata ggagggaagt agcagcatat ttttcataaa gtggctctct
     3781 ttcttcattt ttactttggt tgctcctatt tgaaaattcg agcacactca aatacaataa
     3841 aaagaaatgg caagggagag gattagatga aaaaagaaga aaaaatcgcg attttacaag
     3901 agatcatccg aattaaatca gtgaatggaa acgaaggtga agtagcagcc tatttgaata
     3961 agcttttggc aaaacatgac ataacaggag agattgtttc ttacagagat ggtagagata
     4021 atttgattgc tcgttaccaa aagggccaat caggaaaagt gttaggccta tctgggcata
     4081 tggatgtcgt ggcggctggt gatgaatcgt cttggaccta tgcacccttt gcagcagaaa
     4141 tccatgggaa ccgtttgtac ggacgtggtg ctacagatat gaagagtggc ttggcagcaa
     4201 tggtgattgc catgatcgaa ctgaaagagt caggaaaacc atttaacgga acagtcaaat
     4261 tattagccac tgtcggagaa gaagtgggtg aactaggtgg ggaacagctc acgaaagcag
     4321 gctatgtaga tgacttagat gccttgatca tcggagagcc aaccaattac agtttgatgt
     4381 atacgcacat gggatcaatc aactatacag tcacctcgca cggaaaagaa gcgcatagct
     4441 ctatgccaga ccaaggttat aatgcaatca atcacctaaa tgaattcatt acgaaagcaa
     4501 atgctgaaat gaatcacctc gctgaaacaa tcgagaatcc tgtcctagga aaaacaatcc
     4561 ataatgttac attgatcagt ggcgggaatc aagtcaacag catcccaagc catgcgcagc
     4621 tccaagggaa cattcgatcg attccagaat acccaaatga taaaatcatt gcattgctgc
     4681 aatcaatcgt caatgaattg aatcaagaga cagactatca tttggaattg atgatcgatt
     4741 acaacaaaat acctgtcaaa gctgatccag attctccttt gattcattgc attcagcaac
     4801 aattcagcca gccattacct ttagttggag cggcagcaac aacggatgca gccgaattta
     4861 cgaaagctaa ccattccttc gactttgtcg tctttgggcc tggtgtcgtt actttgcctc
     4921 atcaagtaga tgagtatgtt gaaatcgata attatttgga tatgatcgaa aaatatcaag
     4981 gaattatttt atcctactta gcgtaagatt tttttaataa atgaaaaaac agagccagat
     5041 tagcattatt ttgctaatcc agctctgttt attcagccta tttttctttt tgaatatatc
     5101 aagcgttgtg gcaacatagt gatgactaat gaaacaacga cgatagacaa caaacgatca
     5161 aggtagtctg tacctgcctg taccaagatc acacttgttg tctgacttaa acctaaccca
     5221 tgtaatagct gaacgatcat actggatcct gaggaggtga tcccgccaaa taactgaacg
     5281 gtaatgatcg aagaaacgat cgttccaggt accgacaaaa tcaaagcgag taataacagt
     5341 ttcaagctgt ttttatcttt gccgagatat cttggcaaaa tcaatccagc aagcaagcct
     5401 gtgagaattt ggattggcga ataatacaga gaaaaaatat ctgtagtcac tccacttaaa
     5461 ataccgctgg acaatcctgt cagcattcca ggtacaggcc caagcaatgc accagcaaaa
     5521 atcgttccga tagaatcgag atagattggc aaacgaagcc ataaagcaat cgttcctcca
     5581 atataattga tcccaataca caaagccatc actgtaagta cgcgagttgc cattttgttt
     5641 ttattcattt tgttagccca tctttaacct ttccaaatct ttcataatcg tttcttcttg
     5701 tgcatttaaa agaacggtta aaaactttcg gaaaaaaaga cgagtatcta cttttgtcat
     5761 aattttactg ttagcaggtt tctgccaaaa ttcataacga tcaaccaatg tttgtccacg
     5821 acttattcct tgagtttcca cagcagtgaa gcttttgaaa ccttcacaga tggcctcctc
     5881 gataaaataa gcaacagcta acggatcatt gatgacacat cctaaaattc tttcttgttt
     5941 ccagtggaag tcaaaataaa aacgagtgat cgcttttaga tactctccct cttcaggatt
     6001 catttgacag cagtattcga ggatagtcgg tgtcaagacg atttctcttg tcacatctaa
     6061 cccaaccatt tctatcgttt gtttgagatt ctcgaataca taagaagctg cttcaggatc
     6121 acaccagtaa ttgtattcag caactggcga acagttacca tggcttttgt aagttccacc
     6181 catcgagaca aaacgttcca tatgtttccc aatatttggg ttgactttca aagcagaagc
     6241 gatattggtc aaaggaccca aagcaatgac tgatgtattt gtcttttctt tgaaataatc
     6301 agccaaaaaa tcaacggcat ggagaggttc tgcctgtttt ctcaagatca tcgggaaatt
     6361 tgtttcccct aaaccatcca taccatgagt gtcttgtgca ctgatgaaag gttgcttcaa
     6421 aggtttttcc attccttgat aaacaggaat gtctaaacga tcacagcgtt ccaggcattt
     6481 cagcacattc tgcgtaccta tatctgcagg gacattccca caaactacgg taatagcaac
     6541 tacttcgata tcaggggatt tgacagctaa tgataaagct aaagtgtcgt cgatccctgg
     6601 atcgcaatca ataattactt ttcgcattat ttccaatctc ctttctttag agatacttag
     6661 ttttttattc tgggaagttt ccgaaccagt ccaagacgag cgtcttgatt ataatgcacg
     6721 aaattagtat actggctttt attgcgaatg taaatgctct cttacaagta aatctattga
     6781 ggagaacttt aagtaaatga tacaatgaaa aagaaatgga atttacgtgt gccgaaaata
     6841 aagaatccta tggatcggta cattggataa tagatagatt atagtttggc agaaagcggc
     6901 gggtgtacaa tggttatttt ttatttcatt cttattctga taattatagg gatcagttac
     6961 cattttcttt cttattttta tgcaaagaaa ttagtaagag cgggagtaca gagaggatat
     7021 gactggtatg aagacactgg tcatcgtttg atggaccctg ctgcagtcac gtcagtagaa
     7081 aaaaaacgac aaaaattaga agaagagtgt gaaagctttt gggagcaggg aacacccaaa
     7141 acaatcaaaa gttatgacgg gttgaaacta gctggtcaga tgttcatcca tccaaataag
     7201 cagaataaat gggtcatttg cgtacatgat taccgaagca cagggaaaag agacatgtct
     7261 catataggta aaagatatgc agagaaagga ttcaacgttc ttgtacctga tttacgtgcg
     7321 cacggagaaa gtgaaggaga aatcatcgga atgggatggc tagatcggct agatttaatt
     7381 gcctggatcc aactcatctt agatgaacag ccgaatgcgt ccattatttt acacggcgga
     7441 tcaatgggag cttctacgat catgatggca agtggtgaaa aacttccatc agcggtcaaa
     7501 ggatttattt tagacagcgg atatgtttct gtttatgcag aattccgata tatgttaagt
     7561 aaaatcactg tctttcctaa aaaaatggtc atgagatatg cgaatcatta tgcccagaaa
     7621 tacgccggtt actccttaaa gcaagcatca gctacgagac agcttggcag caatcatctg
     7681 cctttattag tcatccatgg tgaaagagat cattttgtcc ctacagaagc agcgtacacg
     7741 attcaaaatg ctacggcagg ggataaagca ctgctgcttg ttccggaagc agaacacttg
     7801 gaagcatcga tgaaagatcc gaaaacctat tggacagtga ttttttcatt tattgaacaa
     7861 agaataacga tgaattaatt ttctcttctt ttttgtaagt aattcgagga taaataattg
     7921 tttatagatc aggagactgc taaaataagg taggaataaa aaaatatcag ttatttgagg
     7981 agtgcggcat atggaaatca aagaagaaaa aatcgtcttg ctttattcaa tgatgaacaa
     8041 caagagatcg gagaaatgac atggtcagat gcgggcccag acatcatgat cattgaccat
     8101 acatttgtag atccagcata tcgtggtcaa aagttagctg aaaagctagt gtatactgga
     8161 gtagaattag caagacgaga cgggaaaaag atcattcctt tatgccctta tgcaaaaaaa
     8221 gagtttgaaa agaaaccaga atatcaagac gtcttacgtc agtcataaga ataacagctg
     8281 aaagttttga actttcagct gttattccat ttatatagag ttgtcacgga cttttatcta
     8341 tgataaagtt aaagagacga tatcttttgg gaaggaggct gcgatatgta taatttaatt
     8401 ttgggaatcg tgatcgtttt atcgattgtt atggtcattg cgattatgat gcagccaagc
     8461 aagcaaaata gtgcagcgag tgcatttacc ggcggtgcgg atcaactatt cggcaaacaa
     8521 aaagctcgcg gctttgaagc tgtcatgcag cgatcaactg cagttatggg agctatctgg
     8581 atgatcttat tatttgtcct agcatattta tcttcgaagt agttgaataa gcctctcgca
     8641 aaacggcggg cggctttttt tattgcaaag agaacgctta agaagaagtt atgaaagaaa
     8701 ctatggtaaa atgaggcgaa agaggtgaga cgagtgagac aattaccaaa acccctttat
     8761 gcaaaacatg gaaagcgagc ggttttgctg cttcacgctt actcaggaag tccaaacgat
     8821 gttcgaatgc tcgcacgatt tttggaaaaa gcagattata cagtttatgc gccgcttttt
     8881 aaaggacacg gaacaatgga gccatatgat atcttgcaag aaaaagcaga aagctggtgg
     8941 gcagacacta aaaaagccat ccatttttta cagtctgagc agttttctga tattgccgta
     9001 ttaggattat caatgggcgg catctttgct gtacgagcac tagaagaaga atcagtgatt
     9061 ggcggcggtt ttttctgttc gcctttgtct cctgtaaaaa caaacgtgcc tgaaaacttt
     9121 gaaaaatacg tacgtcaagt gctgaagaca gcaggtaaat cagagaaaga aatcaatgaa
     9181 aaagcagttg cttaccgctc gttagctgaa caacaattaa tggatataca agatcaggca
     9241 gcaatcgtag aaagtaggtt gtcagatatc caacagccga tatttttagc tcaagctggg
     9301 aaagatgaaa tgatcgaccc taatggcgta tttgaaactg cacgcaagtt gagccgtcag
     9361 cgagtgacac ttcaatggta tcctgaaagt ggtcatgtga ttacagttgg tacagcaaga
     9421 cgcgaattag aaaaagacgt cttggaattt ttagaaaaat taccttggaa tgaggaataa
     9481 atatgacaaa agaaacgata aaagagaaaa ttctcttttt catggaaagc agtcgcaaac
     9541 gcagtttttc tatggaagaa atcgctgaag gtttagggtt ccaaaaaagc gatgatttta
     9601 aattactagt acaaacgatt gctcaaatgg aaagagaaca gagtatagtt ttcaataaaa
     9661 aaggaaaagt gaaacttcct ttaaaaccaa tcttgatcga aggaacattt cgagcaaatg
     9721 aacgaggatt tggatttgta acgattgatc ccgaagaaga tgacatctat atcccaaaag
     9781 aagcgactgg ttttgcaatg gatggagaca ccgtagcaat cgatatcatg aagcgagcaa
     9841 atacagatat ggatcgcgga gcagagggga aagtagtcga gattcgagac cgggcgacta
     9901 ctcagttagc aggggaattc gttgcttatt cagaagaaga aatacaagaa acagatcttt
     9961 acggcgttgt tatcccgaaa gatagaaagc tcaaccaatt taaagtatat gcagcagcag
    10021 agggagttcg tccagttgac ggaagtattg tgctgatcga actgacccat taccctgaaa
    10081 aagggtatgc gactagttta gaagggataa tcaagcaagt gattggtcat aagaatgacc
    10141 ctggcatgga tatcctctca attgtcgtgg ctaatggtat ccctacgaaa tttcctgatg
    10201 atgtattgtc tgaagcagat gaagttccag atcaaatcag cgaaaaggat ataactggac
    10261 gtagagattt aagaaatcag ctcattgtta cgattgatgg agaagatgca aaagatctag
    10321 atgatgcagt tacggttaaa aaattagata acggtaatta ttttcttgga gtacacattg
    10381 cagatgtttc ttactatgtg acacaaggca gtcaattgga cagagaagca tatgaaagag
    10441 ggacaagtgt atatttgact gatcgagtaa ttccgatgat cccgcaacgt ttatcgaatg
    10501 gtatctgttc actgaatccc catgttccaa ggctgaccat gagttgcgaa atggagattg
    10561 atccaaatgg acaagtagtc tctcatgata ttttcccaag tgtgatccaa acaacagaac
    10621 ggatgaccta cacagctgtc aatcaaatct tagaagatca agacgaagaa acgatgcaac
    10681 gctatgctca gctcgtacct atgttccaag aaatgggtga attgcaccga atccttgaaa
    10741 cgatgcgaat caaacgtggc gcgatttctt ttgaggatcg cgaagcgaaa atccttgttg
    10801 atgctgaagg acacccaaat gacatccagc taagaagcag aggaattggt gaacgcttga
    10861 tcgaatcttt catgcttgcg gccaatgaaa cggtagctga acattataat aaattaaatc
    10921 tgccgtttat ttaccgaata catgaacagc caaaagaaga aaaaatgcag cgcttctttg
    10981 attttgcttc agctttgggc attctggtcc gaggaacgaa aggaacgatc acaccaaaag
    11041 atttacagca tgtgatcgaa ggagtagaag acaaaccaga agctgcagtt atcaatacta
    11101 tgttgctaag aagcatgcaa caagcacgct attcagaaga caattttggg cattatggct
    11161 tagcggcaga ttactacaca cactttacat ctcctattcg acgctatccc gatctaatcg
    11221 tccatcgtct gatccgttct tacagtgaag ataaaagtga aagcaatcaa agttcgtggg
    11281 ctgcggaact tcctgaaatt gcagatcata gttctaagat ggaacgtcgt gcagtagaaa
    11341 cagaacgtga agtagattcc atgaagaaag cagaattcat gatggacaaa gtgggagaag
    11401 aattcgatgg gatcatcagc tctgttgtga aattcggcct gtttatcgaa ctgccgaata
    11461 cagtagaggg attgatccat atcaatgaat tgaaacagga ctacttccat tttgttgaaa
    11521 atcatttagc tcttgtaggt gaaagaactg gtctgacttt taaaatcgga cagaaagtgc
    11581 gagtaaaagt agtcaaagct gatccagatg aacgagcagt agattttgaa cttgtttccg
    11641 cagaagaggt tacagcgttg gaacggccaa aaacacgaaa tggaaaatca ggaaaacgcg
    11701 aaggaaaaca agcaggaaaa cgcccagaaa gaaatggcaa gaagaacaaa cgatatagcg
    11761 atagtaagca aacgttcggt aagaaaaaag ggaagaagcc attttacaaa ggtgttaaaa
    11821 aaaagaaaaa gaaaaaataa aatgagttgg aggaaacagc tatgccaaaa ggcgagggaa
    11881 aattaattgc acaaaacaag aaagctcgcc atgattattc gatcatcgac acgatggaag
    11941 cagggatggt cttgcaagga accgagatca agtcgatacg aaacagccgg atcaatctaa
    12001 aagatggatt tattcgcgtc cgcaacggag aagctttctt gcataatgtt catatcagtc
    12061 cttatgaaca aggaaatatt tttaatcatg atccgttgcg cacgagaaag ttattattac
    12121 acaaaaaaca aatcatccgg cttgaaaatg aattgaaaaa tactggaatc actgttgttc
    12181 ctttaaaagt ctatattcgt aacggctatg ccaaggtatt gattggtctg gcgaaaggga
    12241 aaaaatctta tgataaacgg gaagatttga aacgaaaaga tatcgatcga caaattgatc
    12301 gaacattaaa aaatttctct agataatttc attatccttt ttcgcatatt cggtgctttt
    12361 gcttttactt aatgataaaa tttgttagca tttaatctga taaaacatga ttatcatagg
    12421 ttttttctta gatcaggagc accgtgtata aagaaaaatt cgcttcgttt ttgactgaaa
    12481 taaaaaagtg tctaattttc atgaaaatgt tttgaatttt ctgtttcatg gtggtatgat
    12541 accgtgtggt tagaaaccga ttggaaaaaa ccagatacgc acatgaattg attttatgtg
    12601 aatttcgggg aaaagaggtg aaatggattg gatgaacgct cgctaacgtt ccacattgga
    12661 ccaatttggt ttgatggtac agtttgtata atggtgttac tgacctgttt gattgttttc
    12721 ttcttagctt acttttttac aagaaatctt aagttgaaac caacaggcaa acaaaatgcg
    12781 atagaatggg tcattgattt tagtcgagga atcgtaacag ataatctgcc tagaaaagaa
    12841 ttgagcaatt tccatttgtt ggcatttaca ttgtttttat ttgtttttgt tgcaaacaac
    12901 atcggtttag taacaaaaat cgttttacct aacgaaacaa cactttggaa aagcccgaca
    12961 gcagacccgt ttgttacttt aactttggca tttataatga tcttacttac tcacttattt
    13021 ggggtgaaaa agttaggctt caaaggatat ttcgtaaatt cgttcttgaa accatatagt
    13081 tttatgtttc cgatgaaact gattgaagaa tttacaaact tattaacact tgctttacgt
    13141 ctttatggga atatttatgc gggggaagtt ttgctaacct tgattgcaaa aatggtaata
    13201 agcttaggct ggttctcatt gccgttagca attccattag agatggtctg gatcgcattc
    13261 tccttgttta tcggaagcat ccaagcattt gtcttcgtta ctttatctat ggtttatatg
    13321 agccataaga ttgaagtgga agaataatat tcgttttaaa aacaaactac tatttatcta
    13381 atttaggagg aaaacaatta tgaattatat cgcagcagca atcgcaatca tgggagcagc
    13441 aatcggagcc ggttatggta acggtcaagt tatctctaaa acaatcgaat caatggctcg
    13501 ccaacctgaa atgtctggtc aattccgtac aacaatgttt atcggggtag ctctagtcga
    13561 agctgttcct atcttagggg tagttgtcgc cttgattcta gtttttggtg tttaattgaa
    13621 tcagtattaa ggcagaaaca atttggtttg ttactgcttt tttacatgaa aactagaagt
    13681 acttcaaaga aaggaaggct cgcctatgct gaatcaattg gcaattgcag aagttggcaa
    13741 tgcgatgtta ggaaatgtca tcgttgtcag cggctcattt ttgatactac tggctctctt
    13801 gaagcatttt gcttggggac caatcagcga tattttgaaa aaacgtgaag acaagatcgc
    13861 caatgatttg gattctgcag aacaatctcg catcaactca gcgaaaatgg aacaagaacg
    13921 cgaacaacaa ttgttagcct ctcgttctga tgcagctgat atcatcaaaa atgcgaaaga
    13981 aagtggagaa ttaagccgcc aaaatatttt gaaggatgct caagaagaag cagctcgtct
    14041 aaaaagtaaa gcccaagctg atatcactgt agaacgtgat tcagcgctga actctgtaaa
    14101 agacgatgtt gcagaactat ctcttcaaat cgcggaaaaa atcttgaaca aagaattatc
    14161 tccagaaatg cacgaatcat taatcaatca atatatcgaa ggtctaggtt cttcaaatga
    14221 aactagataa atatacagta ggaagacgtt acgggaaagc tttatttgaa ctggcgatcg
    14281 attcaaatca agcagaagaa atctatcaag atctcgtaag cttacgtcaa atctatgaac
    14341 aagtacctgg attaggtaat atgttaagtg atgttcgtct agaacctcat gaaaaacgag
    14401 aaatcatgga caagcttgtt agtggctatg atggaatcgt taaaaacttt ttagaagttg
    14461 tttatgcgta taaccgaatg gatgatcttt tgttcatgat cgaagaatat gaacgtcgat
    14521 atgatgaaca tcaaggtttg ctgttgggaa gtgtcacaac agcagttcca ctttcagacg
    14581 aacaacgaat acaattagaa aagaatgttg caaaaacaat gaactatcaa tcagtcgaat
    14641 taaaacaaat cgttgattct tctattattg gtggcgcgat tgttgaagct aaccatcgag
    14701 taatcgacgg aagcattcgc acgcaattag aaaatatgcg taatcaatta aatagatagt
    14761 acaaagaggt aggtgaatcg aatggccatc aaagcagaag aaattagtgc cttgattaaa
    14821 gaacaaattg aaaattatca aaacgtgctt tcagtcgaag aaatcggtac cgtaacttat
    14881 gtaggtgacg ggatcgctcg tgcacatggt ttagaaaacg cgatgagtgg tgagttgctt
    14941 gaattttcaa atggctcata cggaatggcg caaaacttag aatcaaatga tgtaggtatc
    15001 attatccttg gcgatttcga gtccattcgt gaaggagata aagtaaaacg aacaggaaag
    15061 atcatggaag ttccagttgg agaggccttg atcggacggg tagttaatcc gctaggtcag
    15121 ccaatcgacg gactaggtga aatcgttact gacaaagctc gtccagtaga agcaatggca
    15181 ccaggcgtta tgcaacgtaa atctgttaac gaaccaatgc aaacaggtct aaaagcaatc
    15241 gacgctcttg taccaatcgg acgtggccaa cgtgaattag ttatcggtga ccgtaaaaca
    15301 gggaaaactt ctattgcgat cgatacgatc atcaaccaaa aaggtcaaga tatgatctgt
    15361 atctatgtag caatcggaca aaaagattct acagttcgta cacaagttga aacattgaaa
    15421 aaatatggcg caatggatta cacaatcgtt gtgaatgccg gtgcgtctca accagcgccg
    15481 ttgctttata tcgcaccata tgctggtact gcaatgggtg aagagttcat gtacaacggt
    15541 aaacatgtct tgatcatttt tgatgatctt tcaaaacaag ctgttgctta tcgtgaactt
    15601 tccttgctac ttcgccgtcc accaggtcgt gaagcttatc caggggatgt tttctactta
    15661 cactcacgct tattggaacg tgcagcaaaa ttaagtgatg atttaggtgg cggttcaatg
    15721 actgccttac catttgttga aacacaagct ggagatatct ctgcttatat cccaacaaac
    15781 gttatttcga tcactgatgg acaaatcttc ttagaaagcg acttgttcta cgcaggtact
    15841 cgtccagcgg ttgatgccgg actatctgta tctcgtgtag ggggttctgc gcaaatcaaa
    15901 gcaatgaaaa aagttgctgg gactttacgt ctagacttag caagttaccg tgaattagaa
    15961 gcatttacac aattcggttc tgacttggat gcagcaacac aagcaaaact aaatcgtgga
    16021 cgccgtacag tagaaatctt gaaacaaaaa ttgcatgcac cgctagctgt tgaaaaacaa
    16081 gtagtgattt tgtatgcttt gacacatgga ttcttagata gtatccctgt agacagtatt
    16141 ttagacttcg aacatgaatt attcgaatat ctggatacca atcattcaga tatctttgaa
    16201 acaatccgta caacaaaaga tcttccagaa gaagaagcgt tgaacagtgc gattcaggaa
    16261 tataaagaca tgttcttagc tacaaaagga aataattcct ctacagaaga taaattgaaa
    16321 tccattcaaa atgcatagtg aggtgagatg aatgggtgct tcattaaatg aaatcaagac
    16381 acgtatcgct tcaacgaaaa aaacgagcca aatcacgcgg gcaatgcaga tggtgtctgc
    16441 ttctaagttg acaaaatctg aagcttcttc acagaagttt caggtttatg caacaaaagt
    16501 aagagaaatc gttacgcatt tgactgctac tcagttaaat gatattgcct cagaaaatcc
    16561 tagaggggat atcaattaca acagcatgct gctcagccgt cctgttaaga aaactggtta
    16621 tatcgtcatc actgcagatg gcggtttagt cggtggatat aacagctcga tattgaaaca
    16681 aacgatgtct attttagaag aagaccacca atcacccgat gattacgtga tgatcgcaat
    16741 tggcggaact ggtgcagact tctttaaagc acgcggtatc aatctagcat atgagctgcg
    16801 caatctatct gatcagccaa gctttgatga agtccgcaaa atcgtaaata tggcaacaac
    16861 tatgtaccaa aacgaagtat tcgatgaatt atacgtatgt tataaccatc atatcaattc
    16921 gctaactagt cagtttcgtg tagaaaaaat gctgccaatc tcagacttag atccagaaga
    16981 agcaactact ttcgaacaag aatatatttt tgaaccttca aaagaagaaa tcttgtctca
    17041 gttattgcct cagtatgctg aaagtttgat ctacggtgcg atcgttgacg ccaaaacagc
    17101 agaacatgct gctggtatga cagcaatgaa aacagcaact gataatgctg ctaatatcat
    17161 tgatgagctg acggtttctt ataaccgtgc aagacaaggg gctattaccc aagaaattac
    17221 cgaaattgtt gccggagctt cggcactaga atagtattga gattggagga aaaaagatga
    17281 gttcaggcaa gattgttcaa gtaatcggtc ccgttgtcga cgtggaattt tctttagacc
    17341 aatccttacc agatatcaac aacgcattag ttgtttataa aaatgacgaa aataaatcaa
    17401 aagttgttct tgaagcagcg ttagaattag gggacggagt gatccggacc attgcgatgg
    17461 aatcaacaga tggtttacaa agaggaatgg aagtcattga tacaggcaaa gcaatctctg
    17521 ttcctgtagg taaagaaaca ttaggtcgcg tattcaacgt actaggagat acgatcgatt
    17581 tagaaacacc tttcccagaa gatgcggaaa gaagcgaaat tcataaaaaa gcaccagcct
    17641 ttgacgaatt aagtacaagt acagaaattt tggaaacagg gatcaaagtt atcgatttgc
    17701 ttgccccata tttaaaaggt gggaaagtcg gactattcgg tggtgccggt gttggtaaaa
    17761 ccgtactgat ccaagaactg atccataata tcgcccaaga acatggtggt atttctgtat
    17821 ttactggtgt aggtgaacgt actcgtgaag gtaatgactt gtattatgaa atgaaagatt
    17881 caggagttat cgaaaaaaca gccatggtgt tcggacaaat gaacgaacca ccaggtgcac
    17941 gtatgcgtgt tgctttgact ggtttgacga ttgcggaata tttccgtgat gtagaaggtc
    18001 aagatgtact gttgtttatc gacaacattt tccgtttcac tcaagctgga tctgaagtat
    18061 cagccttgtt aggacggatg ccttctgcgg ttggttatca accaacattg gcaacagaaa
    18121 tgggtcaatt gcaagaacgt atcacatcta cgaaaaaagg ttcgatcaca tcgatccaag
    18181 caatctatgt gccagccgat gactacactg acccggcgcc ggcaacagca ttcgcccatt
    18241 tggatgcgac aaccaacttg gaacgtaaat taacagaaca agggatttat ccagcggtag
    18301 atccattggc ttcttcttca agtgctttag cacctgaaat cgtgggagaa gaacattata
    18361 aagtagcaac agaagtccaa cacgtattgc aacgatatcg tgaattacaa gatatcattg
    18421 ctatcttagg tatggatgaa ttatccgatc aagaaaaagt gttagtttct cgtgcccgca
    18481 gagtccaatt cttcttgtca caaaacttta acgtggctga acaatttact ggtcttccag
    18541 gttcatatgt ccctgtagaa gaaacagtga aaggattccg cgaaatcttg gaaggcaaat
    18601 acgatgatct tcctgaagaa gctttccgaa gtgttggccg aatcgaagac gtcgtagaaa
    18661 aagcgaaaac actaggctac tagaaagggg tgccccatgg atcaatattt aacagtaaat
    18721 gtggtaactc ctgccggttt ggtctatgat caccatgcag ctatcgtcgt tgcacgtacg
    18781 acagctggtg agcttgggat tttgcctaaa catgcaccaa tcatcgttcc attgacgatt
    18841 gatgaagttc gtgtaaaacg aacagattca gatacccacg tcgattggat cgctgttaat
    18901 ggcggaatca tggaagttcg tgataatgtt gtttcaatcg ttgctgatag tgcggaacgt
    18961 gaacgtgata tcgatgtatc tcgtgcggaa cgtgcaaaac aacgcgcaga acgccaaatc
    19021 gaagaagcaa aagcaaaaca agatacgaac gaattgaaac gggcaactgt tgcgttgcac
    19081 cgagcaatca atcgtatcaa agtatctaag cactcataaa atgagatgcg gatgtttatc
    19141 cgtgtttaag aacagtaaaa agaggttatg acagaagtgg tcagcttcaa gacataagaa
    19201 ggaatttacg aaaattgctt ctcaaatttt tgtgaaatct cagcttattt ttcgaagaag
    19261 ctacttctgg aacaccgtat atcagaggtt gtaaaatcag cgttttgtcc tgagtattag
    19321 agaaacaaat ggaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    19381 agatgattga acaccgttta ttt
//
LOCUS       DAZKCZ010000044        19363 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000044 DAZKCZ010000000
VERSION     DAZKCZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.044"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..634
                     /locus_tag="ACG8KN_001441"
     CDS             197..634
                     /locus_tag="ACG8KN_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188506.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            729..1613
                     /locus_tag="ACG8KN_001442"
     CDS             729..1613
                     /locus_tag="ACG8KN_001442"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9188507.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1837..5004
                     /locus_tag="ACG8KN_001443"
     CDS             1837..5004
                     /locus_tag="ACG8KN_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9188508.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            5072..5569
                     /locus_tag="ACG8KN_001444"
     CDS             5072..5569
                     /locus_tag="ACG8KN_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFP9188509.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5588..6052
                     /locus_tag="ACG8KN_001445"
                     /pseudo
     CDS             5588..6052
                     /locus_tag="ACG8KN_001445"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            6049..6807
                     /locus_tag="ACG8KN_001446"
     CDS             6049..6807
                     /locus_tag="ACG8KN_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9188510.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6893..7633)
                     /locus_tag="ACG8KN_001447"
     CDS             complement(6893..7633)
                     /locus_tag="ACG8KN_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9188511.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7694..8947
                     /locus_tag="ACG8KN_001448"
     CDS             7694..8947
                     /locus_tag="ACG8KN_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9188512.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9269..9496
                     /locus_tag="ACG8KN_001449"
     CDS             9269..9496
                     /locus_tag="ACG8KN_001449"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9188513.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9619..9945
                     /locus_tag="ACG8KN_001450"
     CDS             9619..9945
                     /locus_tag="ACG8KN_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9188514.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            10013..10726
                     /locus_tag="ACG8KN_001451"
     CDS             10013..10726
                     /locus_tag="ACG8KN_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP9188515.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            11036..11887
                     /gene="licT"
                     /locus_tag="ACG8KN_001452"
     CDS             11036..11887
                     /gene="licT"
                     /locus_tag="ACG8KN_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFP9188516.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            12008..13873
                     /locus_tag="ACG8KN_001453"
     CDS             12008..13873
                     /locus_tag="ACG8KN_001453"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9188517.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13887..15330
                     /locus_tag="ACG8KN_001454"
                     /pseudo
     CDS             13887..15330
                     /locus_tag="ACG8KN_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15438..16139
                     /locus_tag="ACG8KN_001455"
     CDS             15438..16139
                     /locus_tag="ACG8KN_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9188518.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16265..16777
                     /locus_tag="ACG8KN_001456"
                     /pseudo
     CDS             16265..16777
                     /locus_tag="ACG8KN_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(17175..17384)
                     /locus_tag="ACG8KN_001457"
     CDS             complement(17175..17384)
                     /locus_tag="ACG8KN_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFP9188519.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            17625..18200
                     /locus_tag="ACG8KN_001458"
     CDS             17625..18200
                     /locus_tag="ACG8KN_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFP9188520.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            18200..18640
                     /locus_tag="ACG8KN_001459"
     CDS             18200..18640
                     /locus_tag="ACG8KN_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9188521.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            18656..19357
                     /locus_tag="ACG8KN_001460"
     CDS             18656..19357
                     /locus_tag="ACG8KN_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9188522.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         6490 a         3220 c         4137 g         5516 t
ORIGIN      
        1 gaactattta aatcaataaa actgttcatc taaatctttt tattagaact aaatcaacag
       61 tagtttaatt actacttgat ttagttcttt ttattttaaa aatttttttt acttagaatc
      121 cagcgaccct agatattttt ctgtataatc ttaaataaag aaaacaatat caaaaaatgc
      181 acgtagaagt agataaatgg agtggttttg caaatttctt ttatttctag aagttattca
      241 cgtatacgga tttgtttttc tcacaaataa aaaggcagga agtacaatgg atcatttgta
      301 tataaaatat attcatcaat tttccgtttt ggtgttaggc acactaatta tagcggggat
      361 tgtcgcattt attattcaag gccgtgacat attaacagta catcaagagt ttttcagtta
      421 tgcatttggt attcctctga tcgctataag gtctgataac aaaaagtgga agaaacgcta
      481 ttacattctc ttgctatttt tgtgttttta tctgccacct aagattttta ctttattagg
      541 tatacaatgc ctatcatttt cttttatttc ctatatcatt gttttaattt tcctcagttt
      601 catagtatgg atgttttatc ttgtcaaaaa ataaatgaat gtttcatgaa cagtacttca
      661 tcacagccta atttttttct ttatgctatc gttatattaa agaaatcaat cagaagggaa
      721 agaaagtgat gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt gaacaagcaa
      781 aggaatttga gcaattagcg atagcaaaat tggaatcggt gtttgatgaa gtggtcgtct
      841 tgcatacaaa aaaagcaggg gatgcaaaaa actttactcg cgaagcggct acggaagggt
      901 atcatagtgt gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc agtggaatcg
      961 ctgaacaaga acatcggcca aatttcggat ttttcccatt gggtacggtg aatgacttgg
     1021 ctcgagcatt agggataccg ctagaacctg aggaagcgat caatcatttt tcgattgaat
     1081 cagtcaagcc attggatatt ggaaaaataa atgacgacta ttttatgaat gtcgtcgcaa
     1141 ttggctctat ccctgaatca atcaatgatg tagatgcgga gaagaagacg aaatttggaa
     1201 aactagctta tttcatgtca ggaatcaaac agttagcttc cacacaatcc tactcgtttc
     1261 acgtagaagt tgatggaaag aaggaagaaa ttgagagcag taccttgctg ataggactaa
     1321 ctaactcagt cggcggtttt gaaacattac ttccaaatgc aaaagtggat gatggtaagc
     1381 tccatttggt ttatttaaaa gattcttctt tgatcgatac gcttaaaaca ttgccagacc
     1441 tattaaaagg agtagatgaa tctacgaaaa atctagccta ccttacttgt gagcaattag
     1501 ttgtgtcatt agctgatgag gaagaattga cgacgaatgt tgatggagat gaaggggata
     1561 aattacctgt gacaatcaaa gtattgccct cacatttaca ggtttattgt taaaatggag
     1621 gttgtgaccg aggagttggc gggagaacac cgcttgttcg ggataaggga ctgtgacaag
     1681 acttttgtca cagccttttt tttgtacaaa aaagaaattt acacctaata gaaaaatttt
     1741 tccataaaaa tacatatttt aaatatgaaa gtacaattgg aaatagatta aaacgataaa
     1801 ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatgtgt aaaaaagctg
     1861 attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt tgccgatgta
     1921 gagaaaacaa acgaatcaac gcaatcagag aatcaaagtt ttgacaagac tactttttca
     1981 gaagaatcga ttacttctga aactacagaa tctcaaagtg aggcttctac taataaaagg
     2041 caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc tgatagcagt
     2101 gaagcggcac tggcaagagc tgtttcagcg gatgggtaca gcgaagctgc cacaacagaa
     2161 gaactggcgc agcttctcgc agacgaatca gtaactaaga tccgactgat tcagcctttg
     2221 actttagaca gggaattaga gatcaaacga gatatcgtta ttgattttgg tggattcgct
     2281 cacaatttcg gcacacacca tatttatatc aatgagatac ctcagtcgat cgagttccag
     2341 aattttaaag gaacagctgc acatccaggt ggattgacac caaatatcga tggaaatgcc
     2401 attatcattg cttatatgac cgatttttgg ggaagcagat atcattttac gggagaaata
     2461 aaatttaccg ggacacttga tttgtatgat gggtccaaat taggactgat ttacgctcct
     2521 cgtgcaactg tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc agtgaaggaa
     2581 ggactgaatg ccgctccggg taaagcctat ttttgtagaa gctatcaatt gaatgtgatc
     2641 aatggtagtc aactttatgg accgtatcta gggaaattct acggttttct agattcagga
     2701 gatgagactg gtagtggaaa aacagcgcca ggaatccata tcttaagcgg ttcaaaggtc
     2761 tctcttgatt atgatcgggc agatggcgtt agcgatgaag gagaggcagt cgatacgcta
     2821 cctggtaatg tcatgtttga agtcagtggt tcgggaagcg aattttcggt aaatacaaag
     2881 atcaatatta ccaatgacaa caatcgcgga atcatccaaa tgagaggttc aggatcgaaa
     2941 gtcactgtat cgaatggtgg gaaaatcacc atcaatacag caactacggg cggatttcgc
     3001 ttgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg
     3061 gctggagatg acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa aggcctttct
     3121 ttgatcgtag aaggagccgg atcagttatc gacatcgata agcagagtgg gcgttccagc
     3181 gcagttcgtt ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg ctcattgagg
     3241 gtaagaaata caggtgatgg caaatcttac gtgaacagac tcaatcaagg aaatcaagcg
     3301 atccaatttt atgacgcttc tggttttcta gaaagtccag gttcagctga ttttacagtc
     3361 actggagaaa aatcaaatat cgatatacgc gcagattccg gagcaactgt agatacaact
     3421 ggagatattg atttaaattt tacggcaggt gaaaaaactt atttagtcat ggtcgggaaa
     3481 acaggggaag ataagaccgg catcatcagt ggggatgtgt tgaatgtgga cttagaatct
     3541 cctacctatt ttgatttcca aaacaaacgg attggaagcg agaccaatgg cataggcggc
     3601 tgggtattcc aaggaaatgg agattcttct ttaaagatcg cccattcaga aatcgcttta
     3661 tggcgaaaaa caggcgcaac attcgataac tttgaaaaag acccggatta tttttctgat
     3721 atgacagact tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag ccctagtgca
     3781 ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa tcgaatcagt
     3841 gccaataatc agctcccgat gattgaatcc ttaagagtgc caacgaatgc agataaaaaa
     3901 atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca
     3961 ggcgaagtag aagtgacttt acgtattact tacgcagata cgacgaaatc atctgtagaa
     4021 attactgctc tcacgcaagg acaaacagat gaaaatgaag gggcaggata taacccatgg
     4081 ggagaaggcg aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga gagattaaaa
     4141 acggaggatc aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca agaagtgaca
     4201 gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc taatttaagt
     4261 accacattga tcaatgcagc ttctcgcacg atcacaggta caggtgaagc aggagccttg
     4321 gtttcattga aaaaaaatca agctgttttg tcagaaacaa ctattgatga tacaggggaa
     4381 tttgttcttt ctatcccaga aaatcaactt tttgcaggag atgtattaga aattgtatta
     4441 aacgaccaag caggagaagc tactgctaag ggtgtgcaga acaagccaag taccaacgat
     4501 gaaattggga atcacaatcc aagtgatacg ccagttgttt atcatgatgc cccgcctttt
     4561 gcacctgcta cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc ttccaaactc
     4621 gattttggtc agatcaaagc aactggtttg acacaagaag catttggcca aatggatata
     4681 caggaaaaat tatggattta tgatcagcga gaaacgaaaa atccgtggat cttaacgata
     4741 aaattatctc agcctttcac aaatgatgca ggattttcta tgcaggatgt cctatatttc
     4801 aagaaaggag aggagtatca attagtgaat gaagaagaac tggaaattgt cagaggacaa
     4861 aattcaacga atgtgcaagc agattattca gaatatttaa ataacggtcg ggcgtttaaa
     4921 cttgtcttaa ccaaagaaaa tcagatagct ggtaattact cagcagagat aacatggaca
     4981 ttagcagatg cgccgacaga ataaagatca aggaggatga ttgatgaatc gaatatggaa
     5041 taaaagtcat cgaaacaact gctctttact tatgatcctt ctccttggat tcatcctttg
     5101 gcagacacct gtccatgcct ccgaattcaa ttttgcggta gattttgaat taccggctaa
     5161 tcagattgat aagaacaaaa catatataaa tgtaaggatg gagcctggga aaacacagga
     5221 aatcccctac acggtcttta accgaacaga taaatcagta acagttgaga cattgatcca
     5281 ctccgctacg acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa gtaaatttga
     5341 tgaaacatta agttatgata tgaaaaactt agcaaaaaca gtgggagaaa taactgttcc
     5401 tcctcaaagt gcggtcactg agacactttc actgaaagcg ccagaaaaag agctggctgg
     5461 tgtggtcgca ggagggatca cgtttcaaga aaaaaaagag aacgtttcta acaaaagtac
     5521 agttggtgtc gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa atagcgaaaa
     5581 agagtcttcc ccagatttga aattaaaaaa agcttatgcg gatcaaagcg atggaagaaa
     5641 tgtgattaag gcgaatcttc aaaatccaaa tgcttcttat atgttcggct tgactacatc
     5701 ggttgaaatc tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa aagaaaaaat
     5761 agatgtagca cccaatacaa attttgattt acttgtccca ttagaaggaa aaaaattagc
     5821 tccgggacat tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct ggaatctcca
     5881 taccgatttt gaaataactg atgatgcagc ggaccaattg aatgaaaagg atattgattt
     5941 aaaaaatcag ccagaaacat ttgactggcg atggctgata cttgttggag tcattcttat
     6001 tggcttattt gctggattat ttattttaaa agaaagaagg aaaaagaaat gaaaaaaaca
     6061 attgcattaa gtaccgttct agctggaagt ctattattat gtacacaaag tgtttttgct
     6121 gagaataaga gctcagccac tacagaagga acgatttctg tgaccaaacc agatgctgca
     6181 aatcctgaag acgttgttcc agttcctcct gttgatccaa atgatcctga tactcctttt
     6241 acacctgacg aaccaaatca gggaacatcc ggattactga cgattgacca agctccagat
     6301 cttgattttg gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc attacaaagc
     6361 ggaacggaca gtactggagc acaaacacaa gtacagaatt tcgtacaagt aacggataaa
     6421 agcggaaatt atgccggttg gaaattatca gtcaaacgaa cagaatttat tggtacaaca
     6481 tctgatacga ataaactaac tggttcaaag gtgaaattca aaaacgccgt gattcgaaat
     6541 agcgcaacta atagcagtgg aatgccatct accgtttctg gaactggcgc tgaaggaatc
     6601 gaaatcccag cagatcaggc agtagatcta gtaactgcag gagcaaatga agggattggc
     6661 acatggattt attcattagg agagaatgtg gaacaaggga gacaatcagt ggaattattt
     6721 gttcctgtat ccaattatgc tgtagacaac tacgtttcaa cattcacatg gtcgttaaca
     6781 gatgctccta cagaagagga agcgtaaaat catatcaacg atttcaggga gtggagccga
     6841 ttcaaggccc actccctgaa atctgctatg cttacgtctc agctcttatt catcaactcg
     6901 aagcagtttt ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc tttcgaccaa
     6961 cgagtaagga gtcaccgttg aaaaattcta acacataatg agcagaatca attttctgca
     7021 attgcgataa gttgatacag taagatttta aaagtaaaaa gtaagcatac ttttctaact
     7081 ctgtcttcaa ttgttttaaa gaagtattca ctaatttatc ttctgtagtt gttttgacca
     7141 taacttggtt tttcaaacct ttgatacttt caatatataa aatttcccga ggatacagga
     7201 cgagacttcc acctttttga tcaataaaga ttttatcgac atttacgatc tgctgttctt
     7261 tccaattgat ctgttctagc acttgacgaa tttgttcaga taaattcctg tcttttctga
     7321 tatatccaat cggaaggatt ttccgattga tgatctctac tgcaaaattc gtataagcgg
     7381 tgaaaaaaac aatattacta tgtgcatttt ttttacgtat ttgttcggcg acatcaatac
     7441 ctgtgagtga ttgcttcaag tcgatatcga taaagaaaac atcgtgatta tcaatagtgc
     7501 ccaactggat caactgattg atttgagaga tagaagtgag ctgcacgata ttctcgatat
     7561 gatgattgac caattctctg atgatccatt cgctgtgaaa ctggttatct tctatgataa
     7621 aatacttttt catatgcagg ctcctattaa aaaaatatgt gtacattttg aaacaaaaca
     7681 atggaggaat aagatggtcg caacagtgac actttatttg acctttactt tgattttaga
     7741 tttaggtatc tatttattct tttcgaaaca actgctagta aaaaacagct ttggttgtat
     7801 agctttgatg gtggtgacac ttttacttcc tatccctaaa gtgagctttc cttacctagt
     7861 tggcttatta ttgatgtttc ttttttattt tactacgaaa aacctgttta tcagctgttt
     7921 gatcagtaat cttttttttg ctatcactgg actcagcttt tttctggttt ttgatctgat
     7981 ggattattta ggaaagagac atcttggaac agcaagtttt tttgcactac ttatcgcatt
     8041 tattttctat ctattagtaa aaagtataga caaacagacg agatttctca acttgctttt
     8101 tacttatcca actgaacact attttggagg tatttgtatt ttattaagct ggggcgtgat
     8161 ttacttggta ttgggaagtg ttcacttccg ctcatctaat tatctgcttc tatctctttt
     8221 gtcgatagtc atcagttttt tttctttgtt tttttccatt atgttgatca gtttccaagt
     8281 aaaagaaaga caacatcagg aatcgcttca actgtatcaa ataaatgaag aatattacca
     8341 tcatctagag gaattcaaac atgattataa aagtctactg ttttcattaa aaattactct
     8401 tcaggaaaat aaaaaagaaa cgttggaatt tttagaaata ttagaagatc attctgcagc
     8461 tatttttgaa aaaccgcaaa tacaacagct aacaaatatt gtatccccag ctgtcagggg
     8521 tgtcttttta aaatttctag aaaaagcaga atcggagaaa atcccgatcc aagtagttat
     8581 tcctgtcgaa gtgaaaacaa tcccgattga tctggttgtt tttattcgct gtttatctat
     8641 ttttatttct aacgcattcg atcatcgtgt agccgatcag agtccgatta cgatcaatct
     8701 gctggaagaa aaagagggat cacgttttag tatagcgaac aaggcaaatc caacgaattt
     8761 gacgctatcg gagatggtca aaagaggaaa aacctctaaa aaaaatggcg ggttaggtct
     8821 atacagtgcg aaaaaaatgt tggatgttta cgaaaatgca gaactaaaaa tcgaattttc
     8881 aaaacagaat cactattttt ttgtagaaat gtatctggca tctagaaaga gcaaatcagc
     8941 ctactgactt actgtcaaag gataagtttg aggaaagtct acacggagat agagacaaca
     9001 acacagcata ttcaaaagaa acagaagcgt gatgaccgtt gtcacccttc tgtttctttt
     9061 gctatgtagt taaacaaagt agttgacact tccgctactt agtattagta cgaaagtata
     9121 cttcatgata ctaagtagat aagaagtgaa aagcggaacc cacgatcgtc aaaatgctaa
     9181 ctgctttgct tacaccagat aaagggaaga gcaatatcgc ttattttagg cgtggagaag
     9241 caagcaaatc cctacgatca caaaaaaaat gctgacccag cagttaagag agttagaaca
     9301 agatgaaagt atccatcgaa aagcctataa ccaagtcccg cctaaagtag agtactcgct
     9361 caccgaagaa ggaaaatcat taagagagat tctagtagcg ttgtctgttt gggaagaaga
     9421 acgtatcgaa aaacaaaaag cctcaaggga agaagacacg ctactgcata cacatggtgg
     9481 ctacctaaat tattgatttt ctatgacatt tattttaaat aaaagaaaac aggagttttt
     9541 ttaaaagcat catctatttt ttatgtaaaa aaatggtatg attaatcaag taagaagtga
     9601 ttggaagaag gcaagcaaat gaatagtatg gaaaaagtca accagtttag agatgaacgt
     9661 aattggcgac aatttcataa cgaaaaagat ttggctatct ctatttcttt ggaagcatct
     9721 gaattgttag aactttttca atggaaaacg ccggaagaag taaaagaaac acaattagaa
     9781 cgaatcaaag aagaactggc agatgtattg atctattcat acatgatggc agacaacctt
     9841 cacctggatc tagatgagat cattgaagaa aaattgatca aaaataattt gaaatatcct
     9901 gtagaaaaaa gcgcaggtgt acgaaaaaaa tacacagagt tgtagaaatg tgtcattcgg
     9961 ctgttggaaa gagcaaaaga aaaacagtaa aatgagtctg gagggagaga aaatgaaaaa
    10021 aaaaggaaaa gcaaaaagat ggctagtcaa tatcctcttg ttcctcttgt tattagtagg
    10081 actagctttg attttcaatg aacagatcaa ggattatttt gtcagagaga caggagataa
    10141 atatgctata gcgaatgtaa caaaagaaga tctgaaaaag aataacgata aagatgtcag
    10201 ttttgacttt gatgcggtag aaccaatgac gacagaaggg gtcatgaggt cgcaaatgag
    10261 aggaacagat ctaccagtga ttgctagtat cgcagtccct tcagtttcaa tcaatttacc
    10321 ggttttcaaa ggcttggata atacgtcact actttacgga gcaggaacat tatctccaga
    10381 tcaagaaatg ggaaaaggaa attatgcctt agcaagccat cgtgcaacga accctgaatt
    10441 gctgtttaca ccgctagaaa atttagagat gggtgccaag atctatttga cagatttaga
    10501 aaatgtgtat acatacaaaa cattttttaa agaaaaagtt gctcctacag atacccaatt
    10561 actgaatgaa gtcgagggaa aagaaattgt cacactaatt acttgtggcg acatggacgc
    10621 cgtcacgcga ctagttgtac aaggagaact ggaaagtgtt acatcgataa aagatgcgac
    10681 agatgatatg agatctgctt tcaatctaga gacaaaaaca ttttaggata ctgggacata
    10741 agtcgttaca gattaaaaac agccaaagga tatgaatcga taaggaaaaa ccaatttgct
    10801 gaaaaaaaga gaaacgcaaa gaggtgcatc tctgatttgg ttgttttcta gatagacttg
    10861 tgttccagtt tttttatttt ttatatgaaa aggcttgcat aaataaaaat tcgatggtat
    10921 gatgattttg aataagaaat ggattgttac tgtacggcag gcaaaaccag aattcagcta
    10981 agaaacagta ggcgaattct aggttttttt atttttcttg agagggcgaa gcaaaatgaa
    11041 aattgataaa attttgaata acaatgtcgt catttcgaaa aatggattcg gggaagaagt
    11101 cgtttgtatg ggaaaaggac tagcttttca aaaaaagatt ggggacgaga tttcgccaga
    11161 agcggtccaa aaagaatttg ttttaagaga ttcttttgct aaaaaccaat tccagcagct
    11221 gatggctgat attccggcag aggagatcga atgggtcaaa caagtagtcg aactggctga
    11281 agataaacta aaagttgaat tttcgcctaa tatttatctg acattgaccg atcatcttca
    11341 ttatgcgatc acacgagcaa aagaaaatat ccaactgcct aatccattat tatttgaaac
    11401 aaaaaaattt tatccgaaag aatatcaagc agcgaaagag gctcttttgt tcgtgaaaca
    11461 aaagacgggt gtggaacttt ctgaggatga agccggtttt attgcctttc attttgtgaa
    11521 tagccaacaa ggaaacgaag acatgcaagt gacgatgacc gctaccacaa tggtaagaga
    11581 tatactgagt atcatcagta aattcttcgg catcgtgttc gatgaagaat ctttgaatta
    11641 tcagcgtatc attactcatc tgcagttttt tgcccagcgt tatctcaaag gggaaggatc
    11701 tgacgagcaa gatgaatttc tctacgcatt agttcaagga aagtatccaa aagcgtttca
    11761 ttgtgtcgaa cggatcaatg agtatcttcg acaaacgcaa aaacaggaaa tggggatcgc
    11821 agagcagatt tatttaacga ttcatattca aagagtagta agtgagaaaa aaagcatcca
    11881 atcataagga ttgttactgt atggcaggca aaacctggat tgaaaaaata ggttcacgaa
    11941 tgtttgtgac tgtttttttc aatctgggtt tttatttttt aaataaaaaa gaaagaagga
    12001 aaaaacaatg gataatcaag cagttggtcg tcgtgtatgg gaagcggtag gaggacaaaa
    12061 gaatgtcaaa agtttagtac attgtgctac gcggttgcga ttcagattaa aagatgaatc
    12121 tttagcagat acgcaaaagt taaaagaaga cccggacgtg atccaagtcg tacaaagtgg
    12181 cggtcagtat caagtagtga ttggcagtaa tgtagcagat gtttatcaat cgatcgtgga
    12241 tgaggaaggt cttgcagttt cggaggatgg aaaagaagag tccaaaaata ttttgaatcg
    12301 tctgatcgat attatctcta gtatatttac accttttcta ggggcgatgg cagccgccgg
    12361 ggtattgaaa ggattcttat cattggcaac ggtgatgggc tggctgacag ctgatagcgg
    12421 tacttatcaa atattgtttg ctgcagcaga tggtgtcttt accttcttac ctgtcatgct
    12481 tgcttttaca gcagctaaaa agttcaaagc caaccagttt ttagcagtag caattgctat
    12541 ggcacttgtg tatccagcta tcacagcagt tgctggtgcg ggagaagcta tcagtttctt
    12601 tgggatccca gtgattcttt caccatctgg ttatacttct tctgtcatac caatcattct
    12661 agccgtatgg gttcaaagta aattagagcc attcgtaaaa aaagtgattc cgcaatttct
    12721 gcaaatgatt ttagttccgc ttgtcgtttt agtagtgatg gttccattaa catttcttgc
    12781 tttaggacca atcgggacgg ttgccggtaa tgctttaggc ggtctcttca actcgattta
    12841 tggatttagt ccaatcgtag caggtctaat catgggaagt ttatggcaag tattcgtgat
    12901 gttcggcatg cattggggat tcgtaccgat catgtttttg aatatcgaac aatatgggtt
    12961 tgatgtattg atgccaatgc ttcttccagc catcttggca caaggtgggg ctgctttagc
    13021 tgtagcattg cgaacaaaag atacgaaact tcgagcatta gggatttctt ctacagtgac
    13081 ttcgttattt ggaatcactg aaccaactgt gtatggtgtg acattacctt tgaagaaacc
    13141 attcattgcc gcttgtattt ctggaggtat tggtggagct attatcggat tttccggagt
    13201 aaaagctttc tccagcagtt tggtcagctt attgacgatt ccgactttca ttaatacggt
    13261 ggatggtgta gagtcaaatg tgacagtagc agtaatagct acaggaattg cttttgttct
    13321 tgcattcgta ggtacattga ttttaggatt tgacgaacaa acacaagaca atcaattaga
    13381 aaacaaacat gcgaatgcag gagaaccgat tacttcagca cggcatacct taaaaagtcc
    13441 tttaacgggt aaagtactgc cattgagtga agtaccagac caagtttttt cttctggcgt
    13501 gatgggaaaa ggtatcgcta ttgatccaga agtaggagaa ctagttgcac ctgcagacgg
    13561 agagatcacg actatttttc caactggaca tgctgtaggg atcacaacga ctgacggagc
    13621 ggagattttg attcatatcg ggatggatac agtagaatta aatgggaatg gctttgagat
    13681 cttagtcaaa caaggggatc tcgtcaaagc aggagattta ttgattcgtt ttgacatcga
    13741 agcaattaga gcagctggct atagcgtaat cactccagtt gtcattacga atactgatgc
    13801 atttgccgat atacttgaat tagaccaaaa agagattatt gcaaacgaag atgttctggc
    13861 aattgtaaaa taaggaggaa atcaggatgc aatcacgttt ttcagaaaaa tttttatggg
    13921 gtggagcaac tgctgccaaa tcaattagaa ggggcttatg atcgagacgg aaaaggacta
    13981 tccgttgcag atgctatgcc tggaggtaag cagcgctttg cgattatcgg cagtgaagaa
    14041 tttgactgga cgattgatca agaaaaatat atttacccga atcatcgagg aatcgatcat
    14101 tacgatcgtt tcaaagaaga tcttgccttg tttgcggaaa tgggattcaa atgttatcgt
    14161 ttttcgattg cctggacacg aatttttcca aatggagacg aaggtactcc taacgaagcg
    14221 ggattagaat tttatgatca gctgattgat gagtgcttaa aatatgatat tgaacctgtg
    14281 atcacgattt ctcattacga aatgcctctt catttggcaa aagaatacgg tggctggaaa
    14341 aatcgaaaac tgatcgactt ctatgaacga tttgctcaga cagtattaga gagatacagc
    14401 agtaaagtca aatattggat gactttcaat gaaatcaatt cggcctttca ctttccagct
    14461 ttaagtcaag gattagtaaa aagcaatgga gcaggagagt accaaaatat tttccaagca
    14521 tggcataatc aattcgttgc aagtagcaaa gccgtaaaaa tcggccacga attgcgttcg
    14581 gatatccaaa tcggctgcat gattatctat gcaacaactt acagcatcga tgccaatcca
    14641 gtcaatcagg cagcaacaat gatccaaaac caagaattca attttttctg cacggatgtc
    14701 caagtacgtg gagaataccc tgcatacaca gcgcgtaccc ataaaaaata tggagtcgat
    14761 ccggaaaaat tagagcaaac ggaaaaagac ttccagttgc taaaagaata tccagtggac
    14821 tacatcggat ttagttatta tatgtctacg gcaatcaatg agactgatcc acaagcagca
    14881 acttcagaag ggaacttgct gggaggagta aaaaatccgt ttcttgaggc aagtgaatgg
    14941 ggctggcaga tcgatccaga agggctgaga atcgcattga atgaattata caatcgttac
    15001 cagaaaccat tattcattgt tgaaaacggt cttggagcca ttgatcatat ggaagaggat
    15061 ggcacgatta ttgatgatta tcggatcgat tatttacgtc gtcatatcga agcgatggct
    15121 aatgcagtag cagacggtgt agatttgatg ggctatacgc cttggggatg tatcgatctt
    15181 gtcagtgcat caacaggaga aatgagtaaa cgttacggct ttatctatgt ggatctagac
    15241 gatgaaggaa atgggacact gaatcgatca aagaaaaaat cttttgattg gtataaagaa
    15301 gtaattgcct caaatggcgt aaacttgtaa ttaaagcagc tgaatgaaaa agaacacact
    15361 tctcacatgt gatttgtgtt ctttttttat tttttatgtg ctattctttt agtaatagta
    15421 aagcaaaggg tgacgttatg aaaaggacac gtgtactata cttggaagtc gcagataaaa
    15481 ttaaagaaga tattttctca ggaaaatatc cagtcggttc gatgttgccg acagaaagtg
    15541 aattagaaga actgtttcaa gtcagcaaaa tcactgtccg aaaggcaatc gaactgctgg
    15601 catcagatga atatgtcgca aagaaaagtg gtcgtgggac gacggttcta agcaatcgtc
    15661 cttacaatcg attatccaaa gcggcaagct ttacgcaaat cttggagaac tcccgatatg
    15721 aagtccggaa agaaacgctg gatttgcaat tggtccatat aaagaaggaa gatcctttgt
    15781 tttccttttt cggtgagcaa gctgtttgtt ttaagcgctt atattatttg aacaatcagc
    15841 cttatattta ttttgttcat tatcttccac aagaaatgat gcaattcagc agagaagaat
    15901 ttggcaaaga atctttgtat cgtttgctga cgaaaaaagg atatactatt gactcatttt
    15961 ctgatgattt ctcggcagta tttttaacag aagaagaaca aggaattctg aaaacagacg
    16021 cacagatcgg catcaaacgg atacggaaat cgtctacgca aattggggaa atgattgaat
    16081 attcggaggc ggtctaccat acggagctgc atccttacca tattgattat gaaacataaa
    16141 taaaaaaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa
    16201 atagcttctc atatttttcg tttggttagg cttattaccg cagatacacg gctgtttctg
    16261 gattaccatt tattctggat aagggggtgt ggcacgattt tcagaaggaa tctatgaacc
    16321 aatcactgta ttgattttag cttcgatggt aaccccattc ggcttgacga tcgcttattt
    16381 cttagggaaa atcattcgta aaaatatact caatcgtcaa gaaatcgata cattgaagac
    16441 cgcttttcct atggggatct gtcagatcac agagggatgt ttccctatcg tattgaacga
    16501 cttggcacgc aatgtcatag caacgggagt cggtgggaca gtaggtggcg gattaagcat
    16561 gttttggggc gcagatagcc gtattccggc atcgggaatg tttgcagtcg ctacgatgac
    16621 cagaccatgg gcattcattg gtgcattgtt agctggttct tttgtcacag gaataaccat
    16681 tttattattg aaaaaaccag tagatccgaa tgcagaaatc atccaagaag aaaaagagga
    16741 agaagacata tcttgggatg atttgacaat tagttaggag aggcagaatg gattacaaga
    16801 aaaaagtaca atagatgtac cttgagacag gaatggtatg aaagaatcag agttagaaag
    16861 aatcgaatat acagattttg gtttaggagc gatcgaaact gtccagagca tcggcaccct
    16921 ccttgaaatg acgatgaagg aaaaatgaga acctttcgtt gtcgaaaggg tctggtatac
    16981 cttatgtcga aggaaaggaa acaaaaaaac tttcctgtac tattccaaaa aggaaagaaa
    17041 gttattatac agtaaaaaaa gaaattattc ttcatgctag agatcagtac acgttcgagc
    17101 ccaatatcaa acactagttc caggcaggcg caacaggtgc ggttatcact gaattttctt
    17161 ccagcagtga cgattcaagt gatattttta caaatccagc tatttatcgt taacaaaaag
    17221 atttttagga gaagctgatg aacaaaaatg atcaaatttt gatcgtttag ttcgtcggct
    17281 tttttattta gagaaacatc atttatcagt gaaacataac acgagaacaa aatgattttt
    17341 tcgttaataa aagaaacaag tatttgttgt ttaaaataat acacgttatt ggcatatagg
    17401 acgaaatccc agtatcttct ataagtaagt catttcatat cgatgaaaac acgttattta
    17461 tttttgtttt atctataaaa tggctgaaaa atgatttttt tctaaatact atatatagtg
    17521 gcgtctatcc tatataccac taaaaaacta caaaatctag tcttttattt taaaaaaaca
    17581 cgtgtagaat gaagatagac ttttgagtta gggagtgttt ttttatgaaa cttactgttt
    17641 tttgcggttc ccgttttggt aataaagaaa gttacaaatt tattgcgcaa acgttaggaa
    17701 aatacatggc acaagaagaa atcgaactgg tttacggtgg ttcatactcc gggattatgg
    17761 gagtgattag taaaacagta ctggaaaata acggaaaagt cacaggtatc tatccgaatg
    17821 ggctttttga aactgaattg cctggaaaag atgttactac atttattcct acagaaacga
    17881 tcgatgaaag aaaggtatta ttatttgaaa agggagatgc tgtcctcgtt ttccctggag
    17941 gtctaggtac attggaggaa ttctcacaac ttctttcttg gatagcaatc ggtctgacgc
    18001 ccgacaagcc aatcggaata ttgaatattg gcggttatta cagcggcttg caacaattat
    18061 tagaaacttt tgcaaaagag gattttatgg ataaaaaatg gttggatcaa gtaatctttt
    18121 ccaacaatcc attggaactg gtcaatgaat tacgagcagt atctattaaa aatcagttat
    18181 tattgaagga ggcaaaataa tgagtcaaat atatttagca ggaccatttt tttcagagga
    18241 gcaaatcgat cgagtaagtc gtatcgaaaa agcgttggag gaaaacaaaa cagtcacaag
    18301 tttctactct ccacgtcatc accaagaaag caattatgaa ctattcagcg ctggatgggc
    18361 gcaggaagta tacgaaaaag atatggaaga actgacaaat gcagaatttg ttgttgctat
    18421 ccttgacttt gagcatcaaa caatcgatcc aggaacggct tacgaattag gtgtggcaac
    18481 tatgttgaaa aaaccgatga tcattgtaca agaagagacg gtacctacga acttgatgat
    18541 tacacaaagt ctccatacgt atttaaaatc agaccaagct gtacgtgaat atgattttga
    18601 aacattgcct gttgaaactt acgtaggcga gtacttatag aagaggaagg gttgtatgtc
    18661 agtgattttg atttcaggaa cgattggtgc aggtaagagc agtttgacgg atatgcttgc
    18721 aaaagagatt gattctaaac cattttatga aaatgtcgaa gataacgaag tattgccgct
    18781 cttttattca aatcctgagc agtacgcttt tttattgcaa atcttctttt tgaacaagcg
    18841 atttttagca atgaaaaatg ctttagtgaa cgatgacaat gtgctagacc gatccatcta
    18901 tgaagatagc ctgttgttcc atctaaacgc tgatttaggt cgggtaacgg atatcgaagt
    18961 acagcagtac gatagcttat tagatacgat gctaaacgag ttagatgatg ttgctccaaa
    19021 aaaacggcct gatttgatgg tccatatcaa agtttctttg gacacgatgc tagaacgtat
    19081 caaaaaacgt gggcgtgact atgaacagct agaaagcgat gagacgttat atacctatta
    19141 tgaaacgttg aacacccgct ataatcaatg gtatgaagat tttgatgtat gtcctaaaat
    19201 acaagtggat ggggataaat tcgattttgt agaaaatcca gaagatgcaa aaaaggtgat
    19261 ccgtcagatc caagaaaaat tagctgaatt ggaaggaaag cctgctggaa aatattttac
    19321 ggataaccag catgcaaaag gtcaagaatt aggctaaaaa taa
//
LOCUS       DAZKCZ010000045        18424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000045 DAZKCZ010000000
VERSION     DAZKCZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.045"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..514
                     /locus_tag="ACG8KN_001461"
     CDS             170..514
                     /locus_tag="ACG8KN_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9188523.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="ACG8KN_001462"
     CDS             515..934
                     /locus_tag="ACG8KN_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP9188524.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="ACG8KN_001463"
     CDS             complement(1026..1376)
                     /locus_tag="ACG8KN_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188525.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="ACG8KN_001464"
     CDS             complement(1342..2673)
                     /locus_tag="ACG8KN_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFP9188526.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="ACG8KN_001465"
     CDS             2929..3495
                     /locus_tag="ACG8KN_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9188527.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="ACG8KN_001466"
     tRNA            3640..3727
                     /locus_tag="ACG8KN_001466"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /gene="bsh"
                     /locus_tag="ACG8KN_001467"
     CDS             4061..5035
                     /gene="bsh"
                     /locus_tag="ACG8KN_001467"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP9188528.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="ACG8KN_001468"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="ACG8KN_001468"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFP9188529.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="ACG8KN_001469"
     CDS             complement(7450..7896)
                     /locus_tag="ACG8KN_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFP9188530.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="ACG8KN_001470"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="ACG8KN_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9188531.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="ACG8KN_001471"
     CDS             8361..8879
                     /locus_tag="ACG8KN_001471"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9188532.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="ACG8KN_001472"
     CDS             complement(8956..9912)
                     /locus_tag="ACG8KN_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9188533.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="ACG8KN_001473"
     CDS             10078..10884
                     /locus_tag="ACG8KN_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188534.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="ACG8KN_001474"
     CDS             10881..11870
                     /locus_tag="ACG8KN_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9188535.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="ACG8KN_001475"
     CDS             11867..12871
                     /locus_tag="ACG8KN_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9188536.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="ACG8KN_001476"
     CDS             13003..13545
                     /locus_tag="ACG8KN_001476"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9188537.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="ACG8KN_001477"
     CDS             13565..14263
                     /locus_tag="ACG8KN_001477"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP9188538.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="ACG8KN_001478"
     CDS             14284..14496
                     /locus_tag="ACG8KN_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFP9188539.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="ACG8KN_001479"
     CDS             14496..15458
                     /locus_tag="ACG8KN_001479"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFP9188540.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="ACG8KN_001480"
     CDS             15476..15871
                     /locus_tag="ACG8KN_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9188541.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="ACG8KN_001481"
     CDS             16034..16399
                     /locus_tag="ACG8KN_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9188542.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="ACG8KN_001482"
     CDS             16396..18207
                     /locus_tag="ACG8KN_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFP9188543.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..>18424)
                     /locus_tag="ACG8KN_001483"
     CDS             complement(18269..>18424)
                     /locus_tag="ACG8KN_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFP9188544.1"
                     /translation="VSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKA
                     MRKRVSR"
BASE COUNT         5919 a         3123 c         3808 g         5574 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atac
//
LOCUS       DAZKCZ010000046        18225 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000046 DAZKCZ010000000
VERSION     DAZKCZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.046"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..1146)
                     /locus_tag="ACG8KN_001484"
     CDS             complement(40..1146)
                     /locus_tag="ACG8KN_001484"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFP9188545.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRTRYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            1356..2357
                     /gene="ccpA"
                     /locus_tag="ACG8KN_001485"
     CDS             1356..2357
                     /gene="ccpA"
                     /locus_tag="ACG8KN_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFP9188546.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLADRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEGFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2694..5039)
                     /locus_tag="ACG8KN_001486"
     CDS             complement(2694..5039)
                     /locus_tag="ACG8KN_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFP9188547.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5318..6220
                     /locus_tag="ACG8KN_001487"
     CDS             5318..6220
                     /locus_tag="ACG8KN_001487"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9188548.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            6433..8208
                     /locus_tag="ACG8KN_001488"
     CDS             6433..8208
                     /locus_tag="ACG8KN_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFP9188549.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8274..9860)
                     /locus_tag="ACG8KN_001489"
     CDS             complement(8274..9860)
                     /locus_tag="ACG8KN_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9188550.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQEMVEEIE"
     gene            10111..10335
                     /locus_tag="ACG8KN_001490"
     CDS             10111..10335
                     /locus_tag="ACG8KN_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFP9188551.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10476..12227
                     /locus_tag="ACG8KN_001491"
     CDS             10476..12227
                     /locus_tag="ACG8KN_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFP9188552.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12227..14011
                     /locus_tag="ACG8KN_001492"
     CDS             12227..14011
                     /locus_tag="ACG8KN_001492"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188553.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14337..15188
                     /gene="licT"
                     /locus_tag="ACG8KN_001493"
     CDS             14337..15188
                     /gene="licT"
                     /locus_tag="ACG8KN_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFP9188554.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVVQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15285..17165
                     /locus_tag="ACG8KN_001494"
     CDS             15285..17165
                     /locus_tag="ACG8KN_001494"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9188555.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEVEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAISGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(17205..18107)
                     /locus_tag="ACG8KN_001495"
     CDS             complement(17205..18107)
                     /locus_tag="ACG8KN_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9188556.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENKVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT         5610 a         3325 c         3805 g         5485 t
ORIGIN      
        1 aaaaaggtgg gagcggacta gctctcacct tttttactct tatttttcta aaacaagtaa
       61 ttcttttggt gtagtcgtga atggcacaca gccatcgctt gtcacatata cacagtcttc
      121 gatacgtacc cctacttttt caggaatata gattcctggt tcaagagaga agcacatccc
      181 ttcttcgatt actaagtcat ttccttctac aagggaaggg aattcgtgga cggttgttcc
      241 gatcccgtga cctaaacggt gattgaaata ttcgccgtag ccatagctgt tgatcacgtt
      301 tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga cggacggctt cttgtgcggc
      361 aagctgtgct tcaagagtga tgttgtagat tttttcttgg aagtcggttg gtttttgata
      421 agcaattgta cgagtagcat cactgcaata gccattccaa acaacgccaa gatcgaataa
      481 cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta ccatgagggc ttgcagcatt
      541 tgctcctgca aggactaatg tgtcgaaaga catttgtgaa acaccttgtt ttttcaattg
      601 gtattcaatt tctgcaatga tcgcttgttc agtcacgcct tctttcactg ctttaaagcc
      661 aatctcaaat gcaacatcgg cccatttacc agcttcgatc agtttgtctt tttcctctag
      721 agttttgatc agttgtagac gttggatgat ggaagttaga tcagtgtaag aatccgttcc
      781 cgtcaagata tcagagatag catcgaagcg ctcgacagtc aaggcgttct tttcaatccc
      841 tacattgcca ggctgaccat agcgagtacg aagttctttt ccgatgatct cccaagggtt
      901 ttcgctgtct aaatagccgc ggacatcata cgcccaacta ctgttttgag catcttcaac
      961 ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga gggataaaaa gagctagtac
     1021 acgctcatga ggatcacttt cgtatccgga aaagtaagcg atgtggccag ggtcgctgat
     1081 ataagccaat tcgacttggt gttgtgccat ccattgacgt agttcattga tttttacgtt
     1141 attcatttgg caacctactt tctaaattaa ttttgtattc aataattgta ccactgattt
     1201 attaaaagac caacgttcta aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata
     1261 aaacggttgc aattcttgag attatttgat attctatttt agaaatgtaa gacgtatttt
     1321 cagaaaacat ataaaatatg aatgggagaa cacaaatgga aaagcaaaca atcactatct
     1381 atgatgtagc gagagaggca aatgtttcga tggctactgt ttcacgtgtc gtaaatggca
     1441 atccaaacgt taaaccagca acaagaaaaa aagtcttgga agtcatcgat cgcttggatt
     1501 atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa aacaacaaca gttggggtaa
     1561 tcatccctga tgtcagcaat atgttctttg cttctttagc acgtggtatc gatgatgtgg
     1621 caacaatgta caaatacaat attatcttag caaattcaga tggcaacgac caaaaagaag
     1681 tcaatgtatt gaataactta ctggctaagc aagtggacgg cgtgatcttc atgggtcacc
     1741 atatcacaga cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt gtattagctg
     1801 gctctattga tccagatgaa caagtaggta gcgtaaacat tgattatgta tccgcaacaa
     1861 aagatgcagt aaataaattg gcaaaaagcg gcaataaaaa aatcgctttt gtcagtggtg
     1921 cgttgatcga tcctatcaat ggacaaaatc gtctgaaagg ctataaagaa gcattaagcg
     1981 aaaataacct accatatagc gaaggattga tctttgaagc acaatacaac ttcaaagatg
     2041 gtctatctct agccgatcgg atccataata gcggtgcaac agctgtctat gtttcagatg
     2101 atgaattagc catcggtatt ttggatggtt tattagatcg tggcgtgaaa gtaccggaag
     2161 gattcgaaat catcacaagt aacaattcac tactgacaga agtagctcgt cctcgtttaa
     2221 caagtatcac acaaccatta tacgatttag gggcggtatc tatgcgccta ttgacgaaaa
     2281 tgatgaataa agaagaagtc gaagaaaaaa cgattatctt gccttatgga atcgaagaaa
     2341 aaggatctac aaaataataa aaggacaacc attcagaggt tgtgagcctg ccgtttaact
     2401 ctgcgttaca aggagcaatt tcaaaaaaca gcatttcatg tttgatgaat attgtgactc
     2461 gtgattcgga gttggcagga gaacaccgtt tattcgggaa aaggggttat gacaaaattt
     2521 tgttataatc ctttttttgt gtcttaaaaa agagagaaag tggttgaaat tacatttatc
     2581 gtatataaaa caatccgtgt atagatgctg cattttgttc accaccatca aaaaaataag
     2641 ggactgtgac aatactattg tcacaatctc ttattactgt gtattctatt aaatcaatca
     2701 tctttctcat ctgattcttt ggttgcttga ttcgctcttg cataagctga agcggtgttc
     2761 cagtaatctg tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc
     2821 ggtccatttt tcgcccatag cgattcaact gtttttccag gtgtttggat ggatttttta
     2881 ttgacagtaa cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca
     2941 tcagatgata attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct
     3001 tgattgatca gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca
     3061 gtattgtcat cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc
     3121 catgaatctc ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga
     3181 tttccgttta agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca
     3241 ctgcgcatca tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg
     3301 ctttcgtgtt tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct
     3361 tcttcataga cgccgccgtt tgctaaagcc tgaaatccat tggtctgttg aagcgtcgtg
     3421 acatctactg tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc
     3481 atctttgcca agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct
     3541 tgatataagt gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg
     3601 aaggtgttcg atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct
     3661 tgccaagttg ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt
     3721 gcaggtccat aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag
     3781 gcatggttat tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca
     3841 gttctgttat ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac
     3901 atgtatccat aattttctac gccattttgc aatgcagcat agacatcttt atcgattgtc
     3961 gaatggattg tatatccttt attttggatt gtttgctgtg ccttttgata ataagcgtca
     4021 ctgaccgatg aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt
     4081 cttgttgctt cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt
     4141 tgttgtggta gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc
     4201 gtaatttggc cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca
     4261 gaaagatctt cttttagcgc acctgtgttc gtgtatggac tatacgtgat cgggctttgg
     4321 ggtaatccag cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag
     4381 attcctcttg cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat
     4441 ggtgagacat tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt
     4501 gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc
     4561 tgtttgacta attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca
     4621 gatactaacg ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca
     4681 tcctctgtgc tgataattgc agttttcaat aaaggtgaaa ttttatcaga tgaaatagtc
     4741 gtgcgcatca gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt
     4801 gacgtttctg taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg
     4861 tcttcgacga gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc
     4921 aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga
     4981 acaaaccatt tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa
     5041 agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa
     5101 cagacaaggc tgtttttggc aggaatttct tttttaaagg agttaaaacg tgagaaaatg
     5161 atctttttta taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag
     5221 aacagggcaa agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat
     5281 tgttagtaac aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag
     5341 catgagcgga aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc
     5401 gacttttatg atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat
     5461 gaatattgat gccggaaaag cgtacatggc agaaaacggt ctaacaaata tcgtcgttca
     5521 tgccccttat atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt
     5581 ccaatttctg agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact
     5641 tcacccaggt gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg
     5701 cttgaatgaa gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg
     5761 taaaggtacg gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa
     5821 attgaatgaa aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa
     5881 tattaaagaa gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg
     5941 gcttaaagta ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccgaca
     6001 cgccaatatt ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc
     6061 aatgtttgac aatatttcga aaatcttgga aactccctat gtcggcaaag ataaaaagaa
     6121 agcttacgca ccatatggaa aagaaatcgc catgttcaaa gcacaaatat tcgatccaga
     6181 agtgtttcct gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag
     6241 caaaaaagga ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtgcttt
     6301 ttgcagtctt tttcatcatg catcttctcc cccctctttt taaatttaga caaacagggt
     6361 atactggtag aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg
     6421 aaggacgaaa agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat
     6481 acatacttta ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt
     6541 aaagcaatct atggaacaga catgtcggca acctatgatg accagctgct gattcgctat
     6601 gctcaaaata ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa
     6661 ggaatctcct acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt
     6721 ttgttcggca tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatc
     6781 gtgaagaaca agtatcttct tttaagtttc tatttttatt ttttgttttc tccgattagt
     6841 tttacacatg aatattccac tcgaacgtat cgtaatgccc tcgtgattcc ggctgttgag
     6901 atcattatcg cttgtttact agctatttat taccgcaaat acggttctag cagaaaattg
     6961 ctgccatggt ttttagggct ttctgtcgct tttcctttct tttggttcat tcgcgaagat
     7021 tctatctggc ttcttccttt tactgtagta gctttgggta tcagtttcgt ccagatcttg
     7081 ctagggcaag acttttcttt caaagaagta aagaagttaa aaaaacgact ctttcttctc
     7141 tcgaaacggg aatggctgaa actttcattg tttttacttc ctatagttgg cttttccatc
     7201 gtctatcagt ggatcgaata taaaaatgag acaacatacg gtattgcaac catcaatgac
     7261 cgttccagtg gagaatttgg acgtttgatg aaacaactga tccgaataga tgatgaaacg
     7321 aacttgaata aagaaaacag tgagatatgg gtctcacatg ctgcactaaa taaagcatta
     7381 gccgcatctc ctacattcca tactgtttct acccgtattg acgaattata tacgactcac
     7441 gcgtggactc aaggtggaaa agtaaaagaa ttacccggag atatcatctt ttgggcattg
     7501 cgagatgtgg taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt
     7561 tggaaaaaag tcaaccaaga attagaagaa gcctatcaca agggaaccat caaaaagaaa
     7621 aaagagttct atctgatggg atcaggagat ggaaagacga tgagtgatct accaatcgta
     7681 ttcgatttcc ttcaatcagg atacgcttat gcatggcgat ataaagattt ccatcaagga
     7741 ggaaattata gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt attaaacctt
     7801 ccgctaatgg agaattggat cgatagtaat gaaccgagaa cacctaattt ccaattaaca
     7861 aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc aagttctggc tattcccgtg
     7921 ctgattgctt caatagcagg gatattactt atgatactag gaaccgtatt tgcaaaagct
     7981 gatcgtgcct attatagttc ctctttgatt gtggtcaccg gattaatact gacacaattg
     8041 gtttttcttt tcggtgtctc gtggttttgt tcttatgcgc cagaaaaaaa ggattatttt
     8101 ttaagtgtct acactggcgc aggcgtgccg ttaacgcaat tcatcgtagt aacaagcatg
     8161 ctagttttca gtaaattaaa tctgaaaaaa gctttatctt ctaaataaaa agacccttgg
     8221 tagctagatt tttaacctaa ctatcaaggg tctttgtgtg gaaaaatcag tttttattcg
     8281 atttcttcta ccatttcctg caaacgctgt tcatattttt tgtttccttt atataaatca
     8341 ccaatcgtcc atgccaacag gaggaggaca agaacaatga ttacataagc gatgtgatca
     8401 gccaatgcta gttgagcgga agtggcttgc tcgccgaaga aactttcgat ctgtcctggt
     8461 aatcctcgta agttcaagta agttagacta atcacagaga accagccaag aagttttacg
     8521 atgaatgaat ttttaaatcg gctacccatc tccgcttggc tgttggtcat catcagtaaa
     8581 gggatcatcg aaaacggcaa cgcaaaagcc aagaacacct gggattcatt cattaagtta
     8641 ttcaaagcgg tatgttcttc gatcgttcct tttgtacttg taaacaaaac acaaatcaat
     8701 acaggaatca cagaaagcaa tcgtgtgacc agtcttctta gccaaatcgg gatacgcata
     8761 tggatgaatc cttccatgat gacttgaccg gtcaacgtac cagtaatagt agagttctgg
     8821 ccagaagcaa gaagtgctac agcaaataaa gtggacaaga taccagattt tgctacagca
     8881 atcaagatac cattgctcat agctgatgga tcagataaag cttcatacag accaaaaaag
     8941 gaaggatctt gaacagaacc ggttttgaag acagcgacac ccataatcaa tagcagcgca
     9001 ttgactagaa aagccatcga caattggata ttagagtccc aagtagaaaa acgtacagct
     9061 tgtgctacat cttcttcgtc ttggtgatcg atcttccgag tttgtgaaac tgctgaatgt
     9121 agatataagt tatgtggcat gactgttgcg ccaataatcc ctaatgctcc agttagcgga
     9181 gtttcgccac caatgacagg tgagtcggag aatgtatggc tgttagggat aaagccagca
     9241 aataaactgt gccaatctgg gttagacaag ccaacttgat agacaaaaac gaataaaatg
     9301 acgaaaatca agcagacaac cagtgcttcg atcttacgaa acccgacttt tgtcagtagt
     9361 aagagaacga aaacatctaa tacagtaata aagacggcaa tcaccaatgg aatatcaaaa
     9421 agtaagtaga gggcgattgc tgcaccaatg acttctgcaa tatctgttgc cataatggct
     9481 aattcagtca agatccataa aatgacaccc aatgtcttac ttgtgcgtgc tcggattgct
     9541 tgggccaaat ccatttgggt cacgattcct aattttgctg ccatatactg taaaagcata
     9601 gcaatcagac tagacatcaa aataactgac atcaataaat attggaaatt ttgtcctccg
     9661 gtgatcgaag tagaccagtt acccgggtcc atatacccaa ctgctactaa cgcaccaggt
     9721 ccagagtaag caagaagtgt tttaaaaaaa cttttcccct ttggtacatc cactgttcca
     9781 tttacttcag ctaaagataa accatttgta tgttcaataa gtcgatgctt atgaaattgt
     9841 ttgttggatt gattattcaa gaaaaaacca cctttcctat ttatctacga ttacattcta
     9901 cacctcaaaa agaaaaaatt aaagaaaaag attcggtatg cctaattttt tttcgtggcg
     9961 tgtcttgcta gatgacgaaa gaaattttgt tctcctctaa aaaaaccact attttttgca
    10021 tgaaactaaa gaatacgcct ttcttttttc aataaacttt agtaaaataa gtcattgaga
    10081 gcaattcgga aatagaaagg agagatttat atgttatcaa caggtattgt cgtacttatt
    10141 gctattattg ctttgctaat cggcgcagta ggtggctttt tcctagcgcg taaatatatg
    10201 aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca tgttgcgatc aatgatgatg
    10261 tcaatggggc aaaaaccatc tgagaagaaa attcgccaga tgatgcagca aatgaagaac
    10321 caagggaaaa aataggttct gacccaacga ctttttacgg gctgattttt tcagtccgtt
    10381 ttttatttcg taatttgtta gactattcag attattcagc aaaaagacaa gcacagacaa
    10441 tcctaggctc ttgataaaga caaaggagga gtaaaatgtc gatttttaga aagttaggct
    10501 ggttttttcg tcaggaaaag aaacattatt taattggggt tttctcgcta ttcgcagtgg
    10561 ccttggtaca gttagttcct ccaaaagtga ttgggatcat cattgatgag attgccgata
    10621 aaaatttatc agtaaagatc attttcgggt ggatcttcct attgattatc gctgcttttt
    10681 tacaatatct ttttcgttat atctggcgga cgaatatttg gggaagtgca gctcggttgg
    10741 agaaagaact tcggcagcag ttattcaaac attttacaga aatggacaat ctcttttacc
    10801 aaaaatatcg gactggggat ttgatggctc atgcaacgaa tgatttgaat gcgatccaga
    10861 atgtagcagg agctggtatc ttgacctttg cagattcagt gattacgggc ggaacaacga
    10921 tcattgcgat gattttgttt attgattggc gcttaacact gattgcgctg attcctctgc
    10981 ctttgctggc cgtcacttca cgagtgctgg ggtcgaaact ccatgatgct tttcgtgatt
    11041 cgcaagaagc tttttctagt atcaatgata agacacaaga aagtatcact gggatcaaag
    11101 tcattaaaac atttggacaa gaaaaagaag atctagcgga ttttgaagaa aagattgaag
    11161 atgcaattat taaaaacaaa cgagtcaatt ttttagactc cttatttgat ccattcatta
    11221 ccttgattat cggtctgtct tatgttgcaa caattatttt aggtggaaag tatgtcttgg
    11281 aaggcacgat ctcgatcggg cagatggtct catttattag ttatatcggt atgcttgttt
    11341 ggccgatgtt tgcgattggt cgattgttca acgtattgga aagaggaaat gcttcttatg
    11401 atcgcgtcaa tgaacttttg catgaaaaaa cgcatatcgt ggaacaactg ggtgcagtgc
    11461 agaccccggc aaaaggcgag ctgaatgtag ctatttcctc ttttaattat ccgaaagaag
    11521 aatcaccagc attagaacaa atcaagtttg caatccaaga aggagaaaca ttaggtattg
    11581 ttgggaaaac tggtgcggga aaaacgacga tcatcaaatt actgatgcgt gaatacgatg
    11641 attatcaagg gaaaatccaa tttggcggac gttcgatcaa agaatattca ttgaatgctt
    11701 tattaggatc aatcggctat gtgcctcagg atcacttctt gttttcgatg accattcgag
    11761 ataatatccg tttttctaat ccaaaacttt cagaagaaga agtagagcat gcggcttctt
    11821 tagcatttat caacaatgaa atcaaggaat taccaaaagg ttatgatacc ttagttggag
    11881 aacgtggagt ctctttatct ggcggacaga aacaaaggat ttcgattgct cgagcattga
    11941 tcaaaaatcc tgaattatta atattagatg atgcactttc tgcagtagat gccaaaacag
    12001 aagaagctat cttgcataat ttaaaagaaa taagaaaaga gaaaacaact atcatttcag
    12061 ctcaccgttt aagtagcgtg atgcatgcaa aagaaatcat tgttgtcgat caagggaaaa
    12121 tcattgaacg aggaacccat catgagctgc ttgcactagg cggctggtat gaacaaatgt
    12181 gggagaaaca gcaattagaa gcgaagatag aaggaggcga ggcctgatgg atgaaaatta
    12241 tcaatcggaa tggacaaaat cgattccact taaagaacag aaaaagatca tcaaacgact
    12301 gatgaagttc gccaaacctt ttagaagaac gttcatcgtt gctatcttat ttgcgtttgc
    12361 tctgtctgtg atcaatgtcc tattgccaag gatcatccaa acttttatgg atgatcatct
    12421 ggctaaacag tcggctacga cacaagttat tttgtttttt gctggtatgt acttatttgg
    12481 agtcatagta aaaagtatca tttggttttt ccaatggtat ttgtattcca tggcttcatt
    12541 gaagacctat cagtatatta gggtaaaatt atttgaaaag ctgcatacac taggtatgcg
    12601 ttattttgat cagacaccgg caggatcaac cgtttctcga gtgacgaatg acacagaaac
    12661 gctatttgaa ttttggtacg tctttttgat ggtattgact gggatctttg cagtcgtttc
    12721 ttcattcatt gccatgtttc aaatcaatgg agaaattgcc ttgtggactt tggtgttctt
    12781 gccaatttta ggaatagtca tctggtatta ccagaaattt agttctcgta tttaccggaa
    12841 tatgcgggaa aaattaagtc agctgaatac gaaattgaat gaatcgattt ccggcatgcg
    12901 tattatccag cagtttcgcc aagaagctcg tttagctaaa gaatttgaac aagtgaacaa
    12961 tgagtattta gacacgcgtt atgcgatgat caaaacaaat tcgattctgt taagtccgat
    13021 catcaatttt ctatatgcat tggcgattgc gttggtgttg actttgtttg ggatcgatgc
    13081 gttgcattct cccgtagaag ttggactgat ctatgcattt acgacctatg tccagggatt
    13141 ttttaatcca atgtctcaaa tgatggactt tttgagcatc ttcacagatg ggatggttgc
    13201 tggtagtcgt atcttgaaga ttatggatac agaagaatat gcaccacaac aaaaccctgg
    13261 agcagatcaa atgattactt caggtaagat tgagtttcgc aatgtaagct tttcttatga
    13321 tggagagcac aatgtattaa acaatatctc ttttgttgct aatccgggag agaccgttgc
    13381 attagtggga cacaccggaa gtgggaaaag ctcgatcatc aatgtgttga tgcggtttta
    13441 cgaattttat gaaggtcaga tattgattga tgatcatgac attcgagaat atccaatgga
    13501 agagttacgt aagaaaatgg ggctggtctt gcaagatgct ttcttgtttt atggggatat
    13561 cgcaggaaat attcggatga tgaatccagc tatcacagac gaacagatac gccatgcggc
    13621 tgagtttgtt caagcaaaca aattcatcga tgaattacca aaaggatatc actcaaaagt
    13681 gatcgaacat ggtgccagct attctagtgg acaacgacag ctgatcactt ttgcgcgaac
    13741 aatcgtgact gatcctaaaa ttctagtatt ggatgaagca acagcaaata tcgatactga
    13801 aacagaagga ttgatccaag aaggattggc gaaaatgcga caaggacgga caacgattgc
    13861 gattgcccat cggctgtcaa cgattcgaga tgcagaattg attttagttt tagacaaagg
    13921 tcatatagta gaacgcggga atcatgaaac acttctagcg caaaatggtt tgtatgcaga
    13981 tatgtacaaa ctgcaaagca gtggtatata aaagatgttg tgacagtagc attttgacct
    14041 aagcactaga gtgaaaataa aacatagctt cccatatttt tcatctgtga gagttagtgt
    14101 cgtagttacc agatttaaaa aagacgacgt cagctagtca gaagtgccta atagccccta
    14161 agaaagcgtt gacaaaatcg tttgctttcg ataatataaa gaaaacgata caacttggat
    14221 agtgattgcc tgatgcaagc taaacctaat gagtctccca tggtttcatg gacggctttt
    14281 tgggtttttc tttttgtttt ggatacagcc ctatatatca tggaaaggag caaaacatgc
    14341 tgattgagaa agtactgaat aacaacgtcg taataacaag ggatgaaaga gatcgggaaa
    14401 tgattgtgat gggaaaagga ttggcattca aaaagaaggt gggtgacccg attcttgatt
    14461 catgcatcga caaaaaattt cagttgacaa atgacggact ttcacaacag ttccaagaat
    14521 tggcacaagc gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa
    14581 aagaggctct gcaagtccat ttaaatgatt ctgtgtatat ttcattgacg gatcacttgt
    14641 atacagctat cgaacgtcaa aaaatgggga tctttgtgcc aaatgtattg ctgtatgata
    14701 tcaaacaact gtttcctaaa gagtatgcca ttgggaaaaa aacagtgaag aaaatctcgg
    14761 ataaatacca catcaagtta tcagaagacg aagccggatt tatcgcattg catttagtca
    14821 atgcacaagg cattcaaagc cagcaagaca atacctacct gtttaccaca acaatacagg
    14881 atattttaaa tatcgtccgc tattactata agattacatt tgatgaagat tcagtccact
    14941 tttttcgatt cgtcacacac ctgcgttttt ttctgacacg tgttctagat ggagcgaaat
    15001 cagaagatgg agaagtttct gaggaattgt tccaagttgt tcaaaaaaaa tataaggata
    15061 cgtttggttg cgttgagaaa atagcagact ttttaaccgg ggctttcgac taccagatgt
    15121 ccacggatga aaaaatgtat ttaacgatcc atactgcgcg tttagtgcat gagaatcgtt
    15181 gtaaataaat aaaaaaggag gacaaattaa caagaacgta tgaaaataag ggtagtgaca
    15241 ttcagttggc taaaccttcc actaattaat tggagggaat agaaatggga aaataccagg
    15301 aattagcaga aaacatcgta aagaatatcg gaggaaaaga aaatatccgt agcttgacac
    15361 actgtgtgac gcgacttcgt ttcaaactaa aggaggaaaa taaagcaaag gatgatgttt
    15421 tgaaaaagat ggaagggata gtcaccatca tgaaaagcgg cgggcaatat caagttgtaa
    15481 tcgggaatca tgtgccagat gtctatgcag aagtactgac agtagccggg atttccgatg
    15541 aagtagaaga agaggtttct tcaagtagta atctgttcaa tcgattaatc gacatattaa
    15601 gtggctgttt ccagcctttt ttaggtgctt tagctgcagc gggaatggtc aaaggattaa
    15661 atgctttgtt cttgtttctc aaattttata ctgaaggatc gggtacgtac atcatgctga
    15721 atgggatagg ggatgcaatt ttttatttca tgccagtgat cttagggtac acagcagcga
    15781 aaaaatttca tctgaatccg atggtggggc tcgtgattgg tgcagcactt tgttatccga
    15841 ctgtacaagg agcagcacta caaagtggtt tccctgatgg cagtcctgcg ccttatagct
    15901 tgctgggtct tccggcttat gaaacatttt tgggtatccc atgggtgggc agcagttata
    15961 ccagtagcgt cgtacctgtt atcttcatta tcgcatttgc cggacaagtc caaaaaattg
    16021 ccaagaaatg gattcctgca gtcgtccaga cctttttggt tcctttttct gtgttattaa
    16081 tcgcacttcc tgctggcttt ttgattatcg gtccgattgt cagtatactg acagatctat
    16141 taagcacggg attccaagca ttgatggact tttctccagc tgtgtatggt gcagttctag
    16201 ggtttctttg gcaagtactg gtgattttcg gacttcattg gagtattatc cctcttgcga
    16261 ttatgcagct agcgcaaaat gggtggagtc agatgttagt aggcgtttat ggcgcgagtt
    16321 ttgcacagac agcagtggta ttggcgatgt atttcaagtt caaggatcaa aaaatgaagg
    16381 cgctttgcat gccagctgtg atttcaggaa tcttcggcgt aacagaaccg gctatttatg
    16441 gaatcacctt accgaagaag aagccattta tttactcgat gattggtggt gccatttcag
    16501 gaatctattt gatggcatct ggaacaatca gctacacaat gggcggatta gggatttttg
    16561 gggtggtaaa ctacatcacg gaaacagacg ccagtggtat gtttcaagcg tttatcggca
    16621 ttgctattgc ttcggtaatc gggttttcgc tgactttttt cttttggaag gatgaagcag
    16681 gtgaaacaga tgctatacag aaaaaatcag gtaaagaaat tgtaaaaaca ccgatacaag
    16741 gacaaatcgc tccactaaat gcagcgaagg atgcagcttt tgcacaagga acactaggtc
    16801 gaggaatctt gatctaccct gaaaaaggag aagtacgtgc cccttttgac ggaacagtca
    16861 tgaccctgtt cccgacgaaa catgcaattg gaatggtttc tgaaacaggg ctggaattgc
    16921 tgattcatgt cggattggat actgtgcagt tggaagggaa atattttgaa agcttagtgc
    16981 agcaaggaga aagagtgaaa caaggcgatc tgttgctacg cttcgacaaa aagaaaatcg
    17041 aaaaggaagg atacagttta gaaacacctg tgatcgtcac caattacacg gattatttgg
    17101 atattttgga aaaccaaggg gagacggtcg ggccagacga tgcacttatc accgcactga
    17161 cctgaacggt taggtgaaga atggaatagg cttaactatt ctttttattc cacttcttta
    17221 ttttcaaaat tgaaaggctc tttcatgatg gctgagctga tagggcgttt atcgaatttt
    17281 tgaccgacag aaattagatg gactaaattc tgttgataat tttctgcaaa aacacaacta
    17341 tataacacgt ctagaagata acaaattgat gtattgatcg taaaattacc aatttttgaa
    17401 tacagtcgct cgcgtgtcgt aagtcgcaaa gcggctgtac tcattgcaga tagcgttgaa
    17461 tcaccgatac tggtcaaagc taaaattgga acatgatttt gtttcaatat ttctgcggta
    17521 cgaagaatca ctttattttc acccgtataa gaaatcagga tcccgcacat atcgctcgtg
    17581 caattgtacg cttcatagaa tgattcacca aaagtggtga caattgtgac atccattttg
    17641 atgcgcttca ttttcagcat gaaatcttga gaaatcaaag tattcgagtt actggcgaag
    17701 atcacgattt tttttgcctg gagcagtaaa tacttggctt tttgcagttg gtcatgatga
    17761 atgagagaaa gtgtatcttg aatcgtcatc tgttctaaag aagccatttt atttgcgatc
    17821 gtcataatac cgtctgtggt ggaaaaagga agattcgcat ctacgtcttg aaaattccct
    17881 tcaagatagg ctgtttcagc taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc
    17941 attttttttg ctacacggat gagtgtagac gggtggacat agcatcgctc agcgatctct
    18001 tggatggtca tttcgccaag tgctggagaa tcgcttaaga gaaactggat cacttcattt
    18061 tctgaaggag aaaaggagat ttctttgatt ttttcacgta ataacatgta gttaccttct
    18121 tcttattttt ttacgtaaat tataactttt ttgaaaatgt tttgtaaacg gatttatact
    18181 atataactta aaatagactt tttttgacaa gatatttaca aaaat
//
LOCUS       DAZKCZ010000047        17914 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000047 DAZKCZ010000000
VERSION     DAZKCZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.047"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..489)
                     /gene="comGG"
                     /locus_tag="ACG8KN_001496"
     CDS             complement(130..489)
                     /gene="comGG"
                     /locus_tag="ACG8KN_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFP9188557.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(461..886)
                     /gene="comGF"
                     /locus_tag="ACG8KN_001497"
     CDS             complement(461..886)
                     /gene="comGF"
                     /locus_tag="ACG8KN_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFP9188558.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(849..1196)
                     /locus_tag="ACG8KN_001498"
     CDS             complement(849..1196)
                     /locus_tag="ACG8KN_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188559.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(1168..1638)
                     /gene="comGD"
                     /locus_tag="ACG8KN_001499"
     CDS             complement(1168..1638)
                     /gene="comGD"
                     /locus_tag="ACG8KN_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFP9188560.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(1613..1891)
                     /gene="comGC"
                     /locus_tag="ACG8KN_001500"
     CDS             complement(1613..1891)
                     /gene="comGC"
                     /locus_tag="ACG8KN_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFP9188561.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(1888..2937)
                     /gene="comGB"
                     /locus_tag="ACG8KN_001501"
     CDS             complement(1888..2937)
                     /gene="comGB"
                     /locus_tag="ACG8KN_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFP9188562.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(2894..3823)
                     /gene="comGA"
                     /locus_tag="ACG8KN_001502"
     CDS             complement(2894..3823)
                     /gene="comGA"
                     /locus_tag="ACG8KN_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFP9188563.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            3955..5709
                     /gene="ade"
                     /locus_tag="ACG8KN_001503"
     CDS             3955..5709
                     /gene="ade"
                     /locus_tag="ACG8KN_001503"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9188564.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(5807..6469)
                     /gene="trmB"
                     /locus_tag="ACG8KN_001504"
     CDS             complement(5807..6469)
                     /gene="trmB"
                     /locus_tag="ACG8KN_001504"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9188565.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(6539..7321)
                     /locus_tag="ACG8KN_001505"
     CDS             complement(6539..7321)
                     /locus_tag="ACG8KN_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFP9188566.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(7525..8736)
                     /locus_tag="ACG8KN_001506"
     CDS             complement(7525..8736)
                     /locus_tag="ACG8KN_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188567.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(8729..9466)
                     /locus_tag="ACG8KN_001507"
     CDS             complement(8729..9466)
                     /locus_tag="ACG8KN_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188568.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9604..10038
                     /locus_tag="ACG8KN_001508"
     CDS             9604..10038
                     /locus_tag="ACG8KN_001508"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFP9188569.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            10031..10375
                     /locus_tag="ACG8KN_001509"
     CDS             10031..10375
                     /locus_tag="ACG8KN_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9188570.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            10555..11565
                     /locus_tag="ACG8KN_001510"
     CDS             10555..11565
                     /locus_tag="ACG8KN_001510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9188571.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(11634..12578)
                     /locus_tag="ACG8KN_001511"
     CDS             complement(11634..12578)
                     /locus_tag="ACG8KN_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9188572.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(12562..15282)
                     /locus_tag="ACG8KN_001512"
     CDS             complement(12562..15282)
                     /locus_tag="ACG8KN_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9188573.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(15279..16499)
                     /locus_tag="ACG8KN_001513"
     CDS             complement(15279..16499)
                     /locus_tag="ACG8KN_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9188574.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(16780..17625)
                     /locus_tag="ACG8KN_001514"
     CDS             complement(16780..17625)
                     /locus_tag="ACG8KN_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9188575.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         5530 a         3556 c         2955 g         5873 t
ORIGIN      
        1 tgtttaattc tatcgaacaa ttgacaaaat ttcaatcgtt ttcactgttt tttatttgtg
       61 aaacggcaaa cattttttta ttaaaaacag tgttataaca gaaaagaggg cgttttcacg
      121 aacattccat cattctgtac tagtatctgt atcatttttt acttcttctg tgaaccgaaa
      181 gataaacggt gagacttcca cctctaagaa tagggactgt tgtacccact gatatgttac
      241 agatccagtt gtgtagtaaa aaactccttc cgaactttcg gcaagcgtag actgttccga
      301 taaagccatc tgcttcatta tttttgctat ataataattt ttgattcgta tattaagatc
      361 cattgacaaa cggtagcttt ctaatgctgc catcaataaa aacgtacata aaagaaataa
      421 aagcaagaca gaaaataaaa tagagccctt ataacatttt ctactgatcg ttttttgtcc
      481 atatggcata tccatcttct ccattttcaa agtgtacata aaaagaaatt gtgtgttctt
      541 tttccaataa ttgaagctca gtgatacttg ttaacatagg ttgatgtccg ccggaagttc
      601 ttcttttacg tatcattgcc tgatatcttt cgatgatata ctgtttatcc tcgatcgtgt
      661 agtgcagttg ctgatttttc acagagacat tgtgactgct cttgagttct tcttctaatt
      721 gtgctaaaaa tattagccac tctttttgat ctttacgctg caaaaattca cttacttgga
      781 tggtttgctg aatcaaaaga ttgaatgtga gcaatagact gctcaatatc atcagtccaa
      841 ttagacattc aatcagcgta aatgctggaa ttacttgttt tttcaatact gatcgcttct
      901 ttctccatat tgacaaacac tttttcgatt actccattat tatcgacaat ttggatatcg
      961 tatgggatca tttcttccct tcgctctggt cttttgtgat acatccgctg cgtcttgata
     1021 tcttcataaa tcattcgaat aaatagcagt tcctccccac ttttcttttc tgcctgaagc
     1081 aaaaacagct ggccaaagct cactaagtag acacagacag aagcgatcat aagcgctgta
     1141 agggactcca acaaaacaaa tcctgtttta ttcaattctt tttgtatagc gaccactccc
     1201 tatctggaat tgatacgtaa tcgtccgctc tttttcttcc caataaaatt gataagcttt
     1261 tagtgaactt tcattcccgg ttctagcagc aaaagtaata gaagcatggc gtttcaaggt
     1321 aatctcatca ggaatatcta atacttccca atttatttta tctggattcg gaacatgaaa
     1381 aatgatttga ttctcttcac tattccaata aaagcctgtt tggatctgat tcacaatagc
     1441 gattttttgc gtagaataga ttcgtttttc aaattggccg aaaaattgat agactgccaa
     1501 ttcattcttc catgcagaaa atgcgagaac tggaaaagaa aggatcgttg taagtaataa
     1561 aatcagtaag agtgattcaa acaacgtata tcctgatgct aaaaccttac cttcaaggcc
     1621 gctgatagat ttctgcatgt tctcgtttga tatagccttc attcaacaat tcctccacag
     1681 taggaactct tccattattt tgaagcgcat agagctcttt ttgtgtgtct accaactgga
     1741 tgatcgcttc acgactttct tggtctacat gattcctata acgcgacaaa ttagggacga
     1801 acaacaacac taatacactg atgatcagta agacgattag catttcgatc aatgtaaaac
     1861 cgctataata agatattttt ctctttttca tggcaaaacc tcctctatgc ttccgtaaac
     1921 aggtaataaa acagcggcat aaactaataa aatcaaagcc gctacgccca aaaaaatgat
     1981 tggctgaata aaatgcatcc agtgatgaag acgtttgaaa agctgttttc ttgtccattc
     2041 gctgtaaaaa atcagttctt tacctaaact tccctttgcc tctccctgta aaacaatttt
     2101 actgaattct tctgatagaa aacgataatt ctggaactgc tcagccaatg acatacctgc
     2161 ctccaatcct tgaatcaact tagaggcaag atgctgaatc agactttctt gtttcgtttc
     2221 ctttaagcag actatgattt gccttaattc gaaaccttca tgaaaaagct ttccaatctc
     2281 tagtgacaaa taagccgatt gatacaagga atatatcggt ccaatcaata attgtctgct
     2341 tatccattca ctctgatcaa caggagacaa cttcatcaga cgatacctta ctagtaaagc
     2401 caatagagta accgctagga tgaatagtag aacataccaa tgaaagactt gtatcagctg
     2461 aataccccaa tgctcttgca tgaccattcc tgaaccaata agctgaggca aaacgaacaa
     2521 ccgcatacta aacaagatac ctagcaaaaa ggtgagcaat agcatcggat atgcaagcag
     2581 ctttttcaaa tcattgcgaa atccttcaac aagccgaaac tgtttggcaa tttccaacaa
     2641 agttgtcggc agattcccat gacgttcagc taactcgatc tgtaccagtt gctctgctcc
     2701 atatcctatt tgcgaaagta cctcactaaa cgcttgccct tcctttagct gatgttggat
     2761 atttgccaaa ataacaggtg gaaacgaacg gatcgtgagt aataaataaa gggattcttg
     2821 cagactaaaa ccagataaaa gcagttctcc catggtttgt gcaaactggt tttgctggct
     2881 tcttgttaat cctttagaat agttctgact gccaaatatt ttcctgagct tcttcataac
     2941 tttcctccca tgattttttt actgattctt cctccataaa ttgataactc cataaaacag
     3001 aaggatggtg attggcatct tccaaaagtt cttgataaat gacaccagct aaacattcag
     3061 atagctcttc ttttttcttt gtcagttcat ggattctgac caatgtacct tcaagattgc
     3121 gtgcatgtac ggttgcaaaa acccggtgcc ctgttagtgc tgctcttacc gcagcatgtg
     3181 cagttgattc atctcttatc tcgccaatga ttagcaagtc tggacgatgc cgtaatgcga
     3241 gtctcaaaag tgtatcataa gtctgtgcta ttttcggatt gatctgtagc tgcagaaaaa
     3301 acggttcttc tatctctaca ggatcttcga tagtaatcac ttgcaatttt tcttccaaag
     3361 ccaaccgata cattagagaa gtcttgcctg atccgactgg tccgctgaaa aggtataatc
     3421 cacgtctgac ggttttctgt aaaatagttg caaaatctcg agaactaaag caacgatggg
     3481 gtgtttcaaa agtaccgtat aatagccgaa tcactaaact ctctcgttga aggtaatctc
     3541 ctacagaaga caaccgcaga cgaacttcct gtttttctaa agaataagta aatgcaccta
     3601 actgagcttt tcttttttct ccgatatcca tctgtcccaa atacttaaat cttgagatca
     3661 gttgctgtcc atctgccgaa gagactttct tccagaatgt tcgttttttt ccatagcgaa
     3721 aatagatgta aaatacttct gaagctggca atatatatac gtcttgggca tgtttggcaa
     3781 aactttcact tatcatttga tccgataatc gttttatttc cattattctc ccctccacaa
     3841 aaaacatacg tctttttgat ctttttgttt caaataaaat gaatttacaa aaaaatattg
     3901 gcaaaaaccg aatttatgtt atacttataa aaatttatct tgggaggcac tccattggaa
     3961 acaacattaa ggaaatatat cgaagtagca agtaaaaaaa agcctgctga cctagtgatc
     4021 aagcatgcta agatcgtcaa cgtctttacg aaagaaatca tggaaaaaga tgtagcaatc
     4081 tgcgaaggaa tgatcgtagg aatcggtgaa tacgaaggaa aaagcgtaca taatgctaat
     4141 gggcaatatc tagtgccagg attcatcgat ggccatgtgc atatcgaaag ctcactttta
     4201 tcaccaagcg aattttcaaa agtatccttg cttcatggag ttactactgt agtgacagat
     4261 ccccatgaaa taggaaacat tgctgggagt acaggtcttg agtttatgat agaagatgca
     4321 agacaaacac tcatgaatat cttttttatg ctaccttctt gtgtacctgt aacccctttt
     4381 gagaccaatg gtgcaaattt aaatgcaaaa aaattagccc cttttttaca aaaacccgaa
     4441 gttttaggct tagctgaagt catgaactat caagcagtag caaataatga aacagacatc
     4501 ctagaaaaga tccaattgat gcaccagcat aagaaaaaaa ttgacgggca tgcagctggt
     4561 atcggcatgg aagagttaaa tgtttatcct gcagcaggta ttcggacgga tcatgaagct
     4621 acaactgcaa aagaagctaa agaacgtttg gatcttggta tgtatttaat ggttcgagaa
     4681 gggaccgttg caaaagatct ggcatcgcta ctaccggcga tcacacctga aaattcaaga
     4741 agatgcttct ttgtcaccga tgataaactg atcaatgatt tagtaaaaga aggatcaatc
     4801 gatcatatcg tccgtcaagc tattgcactt ggaatcgatc cattacaagc cattcagatg
     4861 gcaacattga atgcagcaga atgttttgat ttgaaaacac taggtgcgat tgcccctggg
     4921 tatcaagctg atttcttctt aacagacgat cttaccaagc tgccaattca tgaagttttc
     4981 tataagggaa cattagttgt tcaatctggg caactccaag accatttatt tgctgatcat
     5041 tcaaattcat atacgtgttg tctgccaaaa ctaaacgctc aaccattaaa tccacattcg
     5101 ttagagctgc ttctttcttc ttcacaagcc catgtgatcg aaattatccc gaatagtcta
     5161 gtgactaaag atttggtaga aaaagtggac cgacaaggcg aacgattcat tccttcaatc
     5221 gaaaaagacc aattaaagat tgcggtgatc gagcgtcacc atcaaacggg aaacattgga
     5281 ttaggcatag tgaaaggatt tcagctgaaa caaggagcga tagcgacaac gattgcccat
     5341 gactcgcata atctagttgt cgttgggaca aacgacgagg atatgctgta tgctgctaat
     5401 cttttgattc aaaaaggtgg cgggatgacc gttgtcaaca aacagcagga aatagcttgt
     5461 gtttctcttc ctataggcgg aatcatgaca caggaacctt tttcgatcgt ctatgaacag
     5521 ttgaatgaat taataactaa agcacatcaa ctaggagcag ctaaaacctt tgatcctttt
     5581 ttgacacttt ctttcttaac attatctgtc atacccgaat tgaagatcac cgacaaagga
     5641 ttattttcat tttctcgctt ccaattgatt ccaccttgtg gaaaaaccga tgaaataaat
     5701 acattttaaa tcaaataaat cccagaaaat acattacatc tgtaatgttc attttctggg
     5761 atttatttat ggatattaat gtttttatga aagcagaaag ttagtttcac agacttttct
     5821 ttttggtctt atcttgaaat tgagcttcta ctcgataaat acgttgacct tttgaagaaa
     5881 atttttcttc atactccgtc ataatattgc cttcaaattg actttgatgt aaatctaacc
     5941 atacctgttt gaggatcatg ccatattgag aaaaacttgc cagagaatac tcaaacaatc
     6001 cttgattatc tgttttgaaa tggatttctc catttggacg aagaatctgt tcatctacag
     6061 caagaaaagt cttatatgtc aatcgccgtt tttcatgacg tttttttggc catggatcgg
     6121 aaaaattcag atagatttga tctacttcgc tgtcagcgaa atactctgtc aacgctgatc
     6181 catccacatg tagaagtttc aggttaggaa gcggttgttc gatcaacttg tctagtgcaa
     6241 tcgaaacaac actgacctgc atttctactc caatataatt gatttcagga tgggcttttg
     6301 ccattccagt gatgaactgg cctttcccca taccaatctc aatgtggatt ggatgatcgt
     6361 tttcaaacaa ttcattccat tttcccttcc acaatgttgg atcactaatt acaaaattcg
     6421 ggtgagccgc taacatttcc gctgctcccg gacgatttcg gacacgcatt atttatctcc
     6481 attcctttcc tctaattaaa aaggtctggc caaatctccg cagagtttgg gccaaacctc
     6541 agccactgaa aattcgtttt aattgtaaaa tttcttcgtt catccgtcga tgatcttcac
     6601 gtttgcaata tctggtgatt tcttggagca aattcatggc cgcataccaa tgaaattttt
     6661 caagcatttc gtcagtcggg cgtatgccat aactgatcag ccactgattc cagctagaca
     6721 gtggcacata atttcctaaa atcgttgcaa tatccaccgc agggtctgca aacatgattg
     6781 aatcccaatc gactagatat aaataatttt tacatactaa ccaattacga tggtttacat
     6841 ctccgtgtac tactgataag ttttccttat ctattgacgg gatctgctca cgaagaaaac
     6901 gatggacaat ttgcagaaat tgatttttct ggagttcttc cggtaattct ttttcatatt
     6961 tttgcagcat cttctccggc gtcatgacac gaccaccgat acgggtcagc atggttttaa
     7021 gagaatttga atgatgtaaa tggtacaaaa catcaatcac gtcgtttctt tgaccaatct
     7081 cattaggagt cagcaagcga ccttccagcc actcttgtgc tgtcaaagta tcgccattcc
     7141 ccgttcgttt tgtccaaaca agttttggtg taattccttc ccttgaaaga gcagccaaca
     7201 tcggagtcgt gtttcgttta ataaagactt tgtcctcatc acgcatgcct atgtatgttt
     7261 ttcctgtgtc gcctttgatg ggacgtaacc gccactcttg gtctaactga aactccatca
     7321 tgcattctcc cgtcctctta ctctttgttt tgcagtactt attattctag tcgcctgaac
     7381 actacacgtc aagcacatat acgttctttt tcatttaatt gccattcaaa aaaccagctt
     7441 ttctcaaaaa aaatagaacg aacagcattc ttactgtcaa cgctctattt ttcattctca
     7501 tttttaccct acacagcttt gttttcagtc tgacatcttc ttcaaacgag ttggtacata
     7561 tagtttaaca aagagcatag taacaatcaa ataagccgct agcggaaccc atcgctgctg
     7621 cccatctaat acgactgcag caatcaaacc aaaaataacc gacgcgataa ttaataccca
     7681 tgtgagtaga tactgaatcg cctgcttctt ctgattctta tggattggat aaagctgaac
     7741 gagaaccata tagcggaact ggttcgctaa aggtaacagt tggaaaccga tcaaatataa
     7801 aaataagaaa ccgataatca gactaaaata aaagtcgact actccggcaa tcaataaccc
     7861 acctataatt actaatcgga aataaagacc actaaaatca gaacctcttg ccaatcttct
     7921 tgcaaacaag taaagataag tgttttttga ttctctttta atatgagtca atataggatc
     7981 caaatagcgt cttcttttga tctttgccgt aatttcagga acatctgtga ataaattgat
     8041 aaattgatag atctgatgca acctatgttc ttctacatgc accattttct cccaatccaa
     8101 tcctttttct tgttgcttcc acaaaaacca gtaatataga cttgcctgta ctaatgcacc
     8161 tgctattcct gcccatatta gccaataaaa tgatagaaac aaaaagatca gagaagaaaa
     8221 aaaccagcct aatttccaat aacgaataac ttgttgatct agtcgaaaca attctgctct
     8281 cttgatttgc agatgactga atttcaaact ccataatatg atcagataga cgaaaaacat
     8341 cgaaaaggat tgctccgtac taaccaccac taatggcatg gccaatccgc atgccaatat
     8401 caaaaagatc aacggaaaaa tgcaagaata gcgaaaagct cgttccaaat aggtttgcat
     8461 ttctttttct ttaggaagta aaaaaacaat atctgctgcc tctgcaaaag tagccatctt
     8521 acccacgaat aaacagagca tccaaaatac agagattatg agccctccaa ttgcaaacgg
     8581 tggctgcagg gttttcaacc aattagaata atataatccc acaccgccaa tcaagaaaag
     8641 acaaactaaa acaaaatgat cgttcaatac ataacgcata tatttcatca ttttcttttg
     8701 atgtcttgct aaacgttttt gaaaaaaact actcatgtgc ctgctccttt gttaaagcaa
     8761 tatagatttc gtccaaagaa gaatccggca gctgaaattc ttttctcaac atctcaagag
     8821 aaccgtctgc tcgtatctct ccgttatgta agaccacaaa acgatcacaa tatttttctg
     8881 cagtagctaa aatatgcgta gacatcaaaa taccggctcc ttcttttttc atttctacca
     8941 tcaattcaag caatgcattg atagctagtg gatccaagcc taaaaatggt tcgtcgatga
     9001 tatataaaga aggctcgatc aaaaaggcgc ataagatcat cactttctgt ttcattccct
     9061 ttgaaaaatt agctgggaac cagtcaagac gattagataa ccgaaaagtc tccaacaact
     9121 tttctgctct tttcattgct atatccatag gtatatcgta ggccatcgca gtgacttcga
     9181 tatgttctct caatgtcaat tcttcatata aagaaggagt ctctggaata tacccaattt
     9241 gtttgcgata ttcttccgta tttttcttta aggatagatg attgatttct atcgttcctt
     9301 ttgaagcttc cagcagaccg ataatgtgct tgatggtcgt acttttccct gctccgttca
     9361 gtccaatcaa ccctactatc tctccaggct gtacatcgaa actgatatct tttaacacag
     9421 ggagatgtcc gtacccccct gtgacatgat cgataattaa actcattctt tttcctccgc
     9481 catttatttt tatttattat accacgaaga aaatgacggt attttgtttt tttccttaca
     9541 atagctttaa cttatggtaa agttaagctg aagtatttat tctttataga aaggatgttt
     9601 tatatggaaa actgtatttt ttgcaaaatc atcaatcaag aaatcccaag ctataaaata
     9661 tatgaagacg acaaggtcta tgctttttta gacatctctc aagcaacgaa aggacacaca
     9721 ttagttgtgc ctaaacgtca tgtagcagat atttttgaat atgatcccga acttgcagga
     9781 gaggtttttt cgcgtgtgcc taagattgct caagcattgg aaaaagcttt tccagaaatg
     9841 aaaggtctta atatcttgaa taacaaccgt gaactagcct atcaatctgt ttttcattca
     9901 catatccatt tagtccctcg ctacagcaaa gaggatgatt tttctattca ctttgtcaat
     9961 catcaagata gttatggatc ggaagaacta aaagctattc aagaaacaat cgtaaagcag
    10021 gtgtcctgcg atgattaaaa actttttcaa aggactgtta tttggagctg ctgctggcac
    10081 tgtcggaggt ctgttattgg cgcccaggag cgggaatgaa acaagaaaga aactgatcga
    10141 agaattggaa gaagcaacaa ctctgacaaa tgatttaaat aacagcctga atcattttaa
    10201 aagcgcattg atcgaaacaa aagaaacagc tgaatctgtg attcctgctt tccaagaagc
    10261 aatcaaaaaa gatatcgaag attttaaatt ccaagccgaa ccacgtatca cacaaatcaa
    10321 tgaacaagta gaaaaactaa aagctgatct gccagagtat cctgaacaaa tataataatg
    10381 attattttct caacagattt aactatataa gtgagaaaag tttgcgtatt tcctaggaat
    10441 gtgatgaaat tttgaatttt gtttgaaact accacgacaa cgtttttaaa ggaaatggga
    10501 tatggtatag ttaatatata aaaataataa ctaattacag gagtgcaaat tttaatgaaa
    10561 aaaaaatcaa ttattttggc agctactagt gcgttggctg ttttaactct agcagcttgt
    10621 tcaggcgata ctaataaaga tatcgcaacg atgaaaggcg gaacgatcac tgtatctgat
    10681 ttctatgatg aagcaaaact tgaatcatcg aaccaatcct tagtacaacg gatgatcatt
    10741 tataaagtat tcaataacaa atatggcgat aaagtaaccg ataagcaagt ggatgcagaa
    10801 tacgacaaac aagcgaaatc attaggtgat acttttgaat cacaattaga agctgctgga
    10861 tatacgaaag atacttataa agaatatatt cgtaacaatt tagcttttga agctggcttg
    10921 aaagctcacg tagatattac agatgacgat ttgaaaactg cttggaaatc tttccatcca
    10981 gaagtagaag cacaaatcat caaattatca agtgaagatg aagcaaaaga tgtgaaaaaa
    11041 tcagcggatg atggagatga tttctctaaa ttagccaaag acaaatcaac tgatacagag
    11101 acaaaagaag atggcggtaa agttaaattc gactcaacga caacaactat tcctgcagaa
    11161 gtaaaagaag cagctttcaa attgaaagac ggtgagatct ccgatgttat tactacaaca
    11221 aatcctactt cttatgcgac agaatactac gttgtcaaaa tggtgaaaaa ccaaaataaa
    11281 ggcaatgaca tggataaata caaagatcaa ctaaaagata tcgctactga aactaaacta
    11341 agtgacaatg cattcacaac taaagtaatc ggtgaagaat taaaagatgc aaatgtaaaa
    11401 atcaaagatg atgcctttga aaatgtgttg agtgcattca caacaacttc atcaagtacg
    11461 aaagactcaa gtgaaactac agcttcaaca aagagttctg acacaaaatc aactgattca
    11521 acaaaagaat cttcaactga agaaacaact gattcatcaa aataaaagaa aacaaaaaaa
    11581 gcgaagactt atgtctcgct ttttttgttt tgaatgaaaa aaataaattt ttgctataaa
    11641 tcttttggta catagaaact tcggttgtcc ataccgaaaa tccgatcggt gtattttcct
    11701 ggttctgttt gacgaatcga agtcaacatc atctgcatcg aagcatcgat attgtcgatt
    11761 tgatgtaata tttctgcttc cattattctt ggacgtacag gtgagccata ttctaataag
    11821 ccatggtgtg ctaaaaccat atgacgtaat acgagaacat cttcgtcagc ttcgctgatt
    11881 ttcaacgcaa gacaggcttt agttatttct tcgtcaatca ataccagatg accgatcaaa
    11941 tttcctgcaa gcgtatattc tgtcgacata ggaccggaaa gttcaatcac ctttcctaaa
    12001 tcatgcaaaa tgataccagc atacaacaat gctcgattca gctcaggata ttcatcggcg
    12061 attgatttcc ctaagtgaag cattgtcaaa gtatgataag ctaatccact ggcaaatgca
    12121 tgatggtttc tttttgctgc cggaaactca aaaaattgtt tttgatattt attaagtaga
    12181 tagcgaacaa tacgattcca atgcgcattg gtgatctcaa acaacaattg attgatttct
    12241 tcttccattt cttctttctt caaaggggct ctttccatgt ataaactagg ctctcctggc
    12301 tcatctaccc ttgttaagcg catatgaaga atctttactt gagggtttcc ttgataaacc
    12361 tctcttttac cgtttagaaa aacgactgtg ccggctgtaa atttattgat ttcctcctcc
    12421 gaagcatccc agtatttacc atcgatagta cctgaagtat cttggaaagt aaaagcaata
    12481 aatttttttc cgtttttagc gattcttaca tctgcacttt tgatcaggac aaattgttca
    12541 aactgctcat cgattactaa atcacgaatt tttttcatta tttgctcctt ctaattgttt
    12601 tacgatttgg ttcatctcat ggtaatagct taccatttct ttatcagaag aaaaacaaat
    12661 tatttgatac ttttctccaa atttagctaa aagttcagct aattgatact ttcgctgatg
    12721 atcataatga agccagccat catcaatgat gatcgggcat atcgaacggt tagcttctaa
    12781 tgagaggtaa gcaaatctca tcgccataat cagctgatct ttcgtacctg tagataactc
    12841 atagatggga aattctgtat atccgtctgt taaagtcagt gtatcatttt tcagtaatat
    12901 cttctgataa tttccttttg ttaaaatacc taaataatca cctgctgctc ttaacaactg
    12961 gggtaattgt ctttcactca tctctgttga aagatcacct aagaatgtcc cggcgatttc
    13021 aagtgctccc cattcctcca gtaattcctg taattttgct ttttgccggc tagcttgttg
    13081 ataaagttcg tccaatgtgc cgtctctttg caactcttct acttgaagtt ggagtcgttg
    13141 ccgattttca cttgcttctt tttcttcttg ctggagttgg gattgctttt ctttcagttg
    13201 gcgcagtctt acagataatt tctcttttgt cagctcttca ggaaatagtg gagtaagcat
    13261 ttccgataat tcattgtatc ttttcatctg ctgttcggct tgtttggctt tttgtaaaaa
    13321 gacaggtact tccgaaggat aagataagcc tgcttgagtc aattgttcgc tgtattcttg
    13381 tatccatact ttttgtttct ttttcaattg attgatttct tgcttcaata aagtattttg
    13441 ctgatagctt ctggcaaatt tcatctgttc catctcttgt ataaaacggg aaagcaatac
    13501 cattttttct gaaattgtct tatcttgtat cggtaaccat tctttaacaa actcaaattt
    13561 ttggtcgatt tgtcgtagtt tttcttgtat ttcctgttct cttttatgtt tccgctcaaa
    13621 tacgccatat aatttctgat aatctatgac ttcctgtcca tattctttca tgagtttgag
    13681 cgtatccatc tttcctaagt gccgatgatc cgcaaaacga tatacttctt gctgaagaat
    13741 ctgtttttct tctccagttc tcttttgttt ttcttctgct tcagctaatt gagctaaggc
    13801 tagatcaagt tgtcctagtt tttcttgcca cattcctttt atctcagaga aatcattttt
    13861 agttttaatt tttggttgcc ttttgtagaa gaaatagcca atcatgcctg caccaattag
    13921 taaaaggatc actttcaagg gcattggtac aaatatacct gcaatgatta ttgctccgcc
    13981 agccatcagc atatacggtt tagatgaaga gactggtgct atctctgact gattttgtaa
    14041 taatgctgga taggtctctt ccaagtcatt cacttctgct tcaatcatct cttgctgttc
    14101 tttagctatt tggagacgca cagaaatctg gctttctaga tcagcaatcc ttctttcttc
    14161 agccagaaga tgcttccatt cttcttcata taaaaattct tccggtaacg gatttttctg
    14221 ccaatgccac ctttgttcca aaatcttcaa ttctgaattt gtttctctag tctcaatttg
    14281 taatctttgc cattcttcgt ttaattgttc cgctgtaaat tgaagttgct ggagatcttt
    14341 tagttcctgc tcctgctcta aataaaaata atatctggct gactgctgat ccatcccgct
    14401 atgcttttcc agtgttgctt ctaattcttc caaacgctca tttgtttgct gataccgttt
    14461 ggagaaagac tccagatcta aaagaagttg ctcatcagga agacctgtag tgctgttttt
    14521 tttgagagat tgccattctt catataaaga aaaattaagt tcttgctgtc tggctaatga
    14581 aaattgtttg ttcgcctctt ttaattgtgc caataactgc tgctgttgcg actcgtattc
    14641 ctgttcttgt tggatcaact tgccaaaata agcttcctgt tcttgttttt gatgaatttt
    14701 ttcttgcagc tctttccatt catttaattt ttgattgatt agtaattttt ggcctttttt
    14761 cttgaataat tgttgtgctt tctgataata ttcttgccgc ttttggaata cttgagaact
    14821 gcccgtgatg cctaaagaaa tcagcgcatc atgtagctct ttttctctca aatgatctag
    14881 ttccatcaac tgctcttgct ggaaagtaaa tacctgctgg aataactctt tcgtcaaagg
    14941 tgcgatcagt ttttctagta actcatcact tcccacttga tcccccagcc acaccttcga
    15001 ttttccgcga ttttgctgct tgtagcgctc gacagcaaat tcaccatata caggatgttt
    15061 aagccatatt ttgccgccat aggctgagcc gtcttttggt gtataatcct tttttttacg
    15121 gccttttgaa gggaaaccaa ataatattgc ttgaataaat tgataaatcg ttgattttcc
    15181 tgcttcgttt cccccataga tcagctgatt gcctgaataa aagtcaaaac ttttttgacg
    15241 ccattttcca aaagcagcaa tctcgatcct taaaatcttc attcctgatc ctcctcaaat
    15301 aaaaaatctt gattaagcag ctgcttggct ttttcaagtg tttcttcttg atattccggc
    15361 agttcgctca atattctagc cgcttcttga tgactatata cttcatttaa aatttgttgg
    15421 aaatcttgag gtgtctgata gtattgaaat aactgttcca tcaatttagc agaagcagca
    15481 agtggggtct tcgtataaaa agtattctct atgagagaaa tacgccaaat aaagaaatca
    15541 tttatcctat ctgagaattc ctctaacaaa tagtctaata gctctcctga ttgtatccgt
    15601 tccaaaacat ctgtattgag atggtcataa tcttttaatt ttatctcaat taaactaaat
    15661 ccatcctcta cttttgaaag ctgctgacga atgtgaacaa tgacctcttt tgtcgttctt
    15721 gctttcctca atgatatttc ctttgtcttc caatagacct ctgctacttt aatcggatgt
    15781 atttggcaac ttcctttact taattcaact agtagaatag atgtagaagc ttgttccttt
    15841 ttagtatgac cttgaggcgc acctggataa acaattgttt gcttttcatt taagaccgtt
    15901 ggcacatgga tatgccctaa agcccaataa tcgtatccct tttcttgcat ctttgagggt
    15961 tggaaaggag catagtttcc tttttgagag acgccaggtt ccccgtggta caacccaata
    16021 tggtaatctg tgagttctct agaaggaaat tccatcactt tgtctttttg aatccacggt
    16081 tgacgataac taaatccact gagagagaca gtttctccac tttttatcgt tatttttttt
    16141 gtctctaccg tttcggatgt aaataaatgg acatttttag gaaaggagaa ccaataccgc
    16201 tctttttgat agaaatcatg atttccgaat attataaata caggtatatg gttcttttct
    16261 aaccgtttca tctgttctga aaaatgtttc tgtattttta atgaaggacg attttgatga
    16321 aaattatctc ctgcaagtaa aacaaaatca acgttattta ttattgcctg ttcaatgata
    16381 tttgataaaa cggtcagatt cgtcttcaat agtttttcct gtacagtttt atcaagagtt
    16441 gtcagtcctt caaatgaccg atccatatgt aaatctgccg catggataaa tttcaccatt
    16501 aaaaatttcc tccttttttt cttttatcat accattttca atgttttttt tcatcaataa
    16561 ccgaaaattt gttcgtatta tgcaaagaaa ttacgataaa tggccgactt caaaaaataa
    16621 gtttattcga aaaaaagatt gtgacatgat ttttgtcaca atcctaaata tctatgttat
    16681 attcgcttcg tttgacactg aagaagtact atttgtactg gaatttatgc tccatttttc
    16741 cgtatttaga attctatgtt ctatactacc ttaattagtt tatatggtaa gaattctttc
    16801 aaatgcttga gatactccgt ctttatcatt tgaaacagtg acataatcag cttttttcat
    16861 gatttcttga tgactatttc ccattgcaaa actaatatct gcactttcaa acatagataa
    16921 gtcatttaaa ctatctccga tcgcgattct caaaggtttc tttaaattgt agattgattc
    16981 caaacgatca aacaccacac ctttagatac attttgaggt acgatttcaa tatttacagg
    17041 tgccgattca gtaacagaca catttagcat ctttttcaaa tggttaaata tattagcttt
    17101 caattgacga ttaggaataa aaataaaaaa tttgtaaatg ctgatagcat ccttttctat
    17161 aatttcctca atattattat aagggattga acaaatagat ttttcatact caagctcata
    17221 gtttagctct ttgatagaaa attcactccc tacctcgtga aaaatttttt tcattttatt
    17281 tttataattc tgagattcat aacttccgag atttgtatat attttggttg gaaatttcga
    17341 taggacagaa tcaatttttt ttacatcatt tttatcgaag gaagactcaa atatcttttt
    17401 ttctccatca aatccaacag aaccatttaa ccctactgct ggtatcctta aaccagattt
    17461 tttgagaata ttattaatgt cttctacttc tcttccagaa caaatgaatg gaaaacagtc
    17521 ttccggtaaa tgtttcattg ttcttgcatt tttttgacta atgctctggt cagaattcaa
    17581 aagagtaccg tctaaatcta taccaattag tttcatttca tccatgtctc ctatacgttg
    17641 attataaatt cactgcttat tgtaagtcta aatttctcac aaaacatata ctaattatac
    17701 ctaatttaag taagtacaaa catcatttta agaataggaa aggacattta tatactattc
    17761 tctgtatttt tgatttataa ataaatatga attgaatcca aatcagctat attactattt
    17821 tataataacc aactataaaa aacgaagtta ggtgaaaaac taacttcgat attcataaaa
    17881 gactctatta gttcaactta gcaaaagatt taaa
//
LOCUS       DAZKCZ010000048        17794 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000048 DAZKCZ010000000
VERSION     DAZKCZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.048"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..404)
                     /locus_tag="ACG8KN_001515"
     CDS             complement(12..404)
                     /locus_tag="ACG8KN_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9188576.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            complement(491..988)
                     /locus_tag="ACG8KN_001516"
     CDS             complement(491..988)
                     /locus_tag="ACG8KN_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9188577.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(1105..1329)
                     /locus_tag="ACG8KN_001517"
     CDS             complement(1105..1329)
                     /locus_tag="ACG8KN_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188578.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(1433..3343)
                     /gene="ltrA"
                     /locus_tag="ACG8KN_001518"
     CDS             complement(1433..3343)
                     /gene="ltrA"
                     /locus_tag="ACG8KN_001518"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9188579.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(4127..5332)
                     /gene="mobT"
                     /locus_tag="ACG8KN_001519"
     CDS             complement(4127..5332)
                     /gene="mobT"
                     /locus_tag="ACG8KN_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9188580.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(5355..5508)
                     /locus_tag="ACG8KN_001520"
                     /pseudo
     CDS             complement(5355..5508)
                     /locus_tag="ACG8KN_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(5511..6896)
                     /locus_tag="ACG8KN_001521"
     CDS             complement(5511..6896)
                     /locus_tag="ACG8KN_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9188581.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(6925..7311)
                     /locus_tag="ACG8KN_001522"
     CDS             complement(6925..7311)
                     /locus_tag="ACG8KN_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9188582.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(7327..7641)
                     /locus_tag="ACG8KN_001523"
     CDS             complement(7327..7641)
                     /locus_tag="ACG8KN_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9188583.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(7994..8473)
                     /gene="rlmH"
                     /locus_tag="ACG8KN_001524"
     CDS             complement(7994..8473)
                     /gene="rlmH"
                     /locus_tag="ACG8KN_001524"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9188584.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            8901..10190
                     /locus_tag="ACG8KN_001525"
     CDS             8901..10190
                     /locus_tag="ACG8KN_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFP9188585.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(10229..10834)
                     /gene="ytpR"
                     /locus_tag="ACG8KN_001526"
     CDS             complement(10229..10834)
                     /gene="ytpR"
                     /locus_tag="ACG8KN_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFP9188586.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(11030..11494)
                     /locus_tag="ACG8KN_001527"
     CDS             complement(11030..11494)
                     /locus_tag="ACG8KN_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9188587.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(11516..11836)
                     /locus_tag="ACG8KN_001528"
     CDS             complement(11516..11836)
                     /locus_tag="ACG8KN_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9188588.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(11979..13058)
                     /gene="pepA"
                     /locus_tag="ACG8KN_001529"
     CDS             complement(11979..13058)
                     /gene="pepA"
                     /locus_tag="ACG8KN_001529"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFP9188589.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            13258..13608
                     /locus_tag="ACG8KN_001530"
     CDS             13258..13608
                     /locus_tag="ACG8KN_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9188590.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            13793..14746
                     /locus_tag="ACG8KN_001531"
     CDS             13793..14746
                     /locus_tag="ACG8KN_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9188591.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            14759..16546
                     /locus_tag="ACG8KN_001532"
     CDS             14759..16546
                     /locus_tag="ACG8KN_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFP9188592.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            16533..17735
                     /locus_tag="ACG8KN_001533"
     CDS             16533..17735
                     /locus_tag="ACG8KN_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188593.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         5043 a         3531 c         3183 g         6037 t
ORIGIN      
        1 tttcaatata tttaattgga tatgccataa aattcactcc taactgctgt aatggcttct
       61 tgtggctggt ttctgccaag cgttactttt tttcctgcat aggtcagctt tcgtcgcagt
      121 gcataagcaa tgacagactt caaaaatcca taaggctttt taccatcaaa ggtttttgta
      181 gacataaacc atgtgaaagc cacaggaatc ccaaagtatt tgagaaatgc tccctctatc
      241 atagaaagag ggggcaagtt gccaagtatc ataactgcaa agagtgacac gacaaaccat
      301 gtcatttgcg taaaggttat gggaaacgga agtctaaagt cattgatgga gtacaacact
      361 ttctcaacag accagatact ggtatagctt cgtattttct tcatgtaatc aatcctttca
      421 taaaaatggg gatagtcttt tgactacccc gtaaacaaga aaatctgcca gtaagaatgt
      481 accgacagat ttaatagacg atttcaaaaa tcccgtgatt ggttgagata aacgttcccg
      541 aaaggtctaa atcccgacca taggattcat aatcaatata gttttgaaga ctggctggca
      601 cttcgcctaa agcacccgtt tcttcaatgt agtagcgtgc cacgtcagac atatcatcac
      661 aatcggaatg aatgataata tcttcttgat gttcgcttag ttcttcaata cttgaaaaat
      721 gagtgagcag agcagatagc tccgattgta gttcttctgg caactccgat accatttccc
      781 ataggcgatt gagttcgcta atagaagtgt attcgtcaac cgtaaagggt aactcgtaat
      841 catgaatggc gtattcctca tattcatcat tcaagccgat tttctctttg acttcctcaa
      901 agtcaatggg aaaggtaaac cacgcaccga ccaattcgcc ctcattgtat ttgcctaaat
      961 tcgcaatata gacttgcata tcgtccatat attcacgtcc tttctttgta gagattcaaa
     1021 aatccctacc gcacttcgtt tggtgtacca ttcctttgcg gaacataaga aaaccactta
     1081 tattccacaa aagaacggtt ttatttaagc accaataatg cgattgaata gctctagtaa
     1141 aatgtctttt actccagcag cgttgaagac taagccaacc gcaataatcg caataattaa
     1201 aaagccaatc agtttgctaa actcacgctt gaagccaaga tacaagccaa tcacaacgat
     1261 tgctaaaagc accagtgatt gagcgtttga tagaaaccag ttatatatgg ataggtaagt
     1321 tcctgatatt ataaatatct caaacttttc cccccattca cacggagcgt gcgactttca
     1381 ccgcactccg cgttccatcg tgtttatatt gaaaagtatt tatgtagtag acctatatca
     1441 agtttaattc ttgattgcca gctaatactc tatatttatt tagtcttatg agctggactt
     1501 tatttagctt caatagagct atatattttt gtattgtttc ttctgtttta gcgtgtatta
     1561 gacggtgtac cgcctctttt accaaaataa gatttttata atcgtcattt ccaccagctc
     1621 cagttggtac aatatgatga caatgtattt cattagcttg ttgaaattct tccagagtta
     1681 tagcacattt tccatgttga gcagaaaata gcgaaattcg attgtctgcg tattctattg
     1741 tgcgattttc caaagataat ttcattaact ggtacatcag taatttatca acacttagat
     1801 tggtgtggat aaattttctt ccctctagag tgtagcaatt cgctaaatac acctttgcca
     1861 ttggttttct gtgttggata tatccaacag ggaaaatagg ttcgccctcg ccagcaacat
     1921 accgaagcat tttagattta ccatatcttg ttgcctcctt tgtcgtaagt tgtctgcctg
     1981 tttttctcaa tctgcccctt ttgtttgtac ctaatctgtt agtgaagata gccattacag
     2041 ctctattcag tttcatacaa tccagattta cattcgttgc aatctgataa taattttgca
     2101 tacctaatac cattgaatta taaagtcttg tttcttctaa ttccgttttc ccttttgagg
     2161 gagaagcaat tttctttgcc tgtgatacca gcttgtcttt ttggtttttg aattgtttat
     2221 cacttatatg tgatttaaca actaacttct ttcttctcgg tatcatttta atcttgaaac
     2281 caagaaagtc ggagtatctt tttctaacat ttacaattct tgttttttct tgtgatactt
     2341 ccaattttaa tcgttgttca atccattgag tgattgctat tttggctttt tcagcacttt
     2401 ccttgtagcg gcagaaaact ctaaaatcat ctgcataacg aaccatatac atttccttta
     2461 actttgtttt cttcatttct ttgtaagcgt tgcttttaca tgggctaccg cttttattga
     2521 tatgaaccac atagtttttt gtaacaggat tttcctgcca ttggctttct acccaatggt
     2581 ctaactcatt aagaacaatg tttgctagta gtggggatat tattccccct tgcggagttc
     2641 ctttctctgg atatgttatt gtaccgttag tcattttgat tggagctttt aatatccttt
     2701 ttaaaatgaa tatcaagtgc ttgtcccgta ttcccatagc ccatatctgt tttatcagct
     2761 tggaatggtt cacgttgtca aagaaaccct taatatcaaa ttcaatcaca taatgtagct
     2821 ggctgatttg taggcggtta taggttgctt ttattgcatt ttcaactgac ctattaggtc
     2881 taaaaccata gctgttttca ctaaatttag cttcgcagat tggttctaat acttgtttta
     2941 tacattgttg tataagcctg tcccacatac aaggaatacc tagaggtctg gttttaccat
     3001 ttggttttgg tatttctttt cgcctcactg gcttaggtcg atacccatgt ggactgcctg
     3061 caacaatgta tcttactttt ttgacaactg tttctgctgg caatttgcca atatctttaa
     3121 ttattgtacc gtctgttcct gctgttttac tgcctgcatt tgctttgata tttcgatatg
     3181 ccagtaatat attttctctc gataggataa tatccataag atgagtgaaa atatctccat
     3241 tcttactttt ggcatataaa tcatcaaagg tgttttgcat ttcgtaatat tcagcgtgtc
     3301 ttaaatcttc tacacacaat gtttcatttt tatttttagg cataaggctt cactccttta
     3361 cttgaaattg agtgagcttt tttatcttac tcgtaatcct tattgaaaca acataatact
     3421 tttctaatat atacacgact tgagggcatt cctccattct cattacaaga atttcttagg
     3481 ttcgccctct gcttttcagc attagttcaa ctgcttattg ttcttcgtca gtttattctc
     3541 ttgatgagaa cccaatacct ttcctcgttc cgataatctt atctatacat atatatcctt
     3601 aggtcactag ctataagcct gctggcttga taccgcctgt aacgttatat ggcatttcat
     3661 aacctatatt cttactttgc cacaccagct acgatttctc gtacctttgc atttctacaa
     3721 agttcaaatt tagacccgta cattcgctgt ttcgtcagtt ccttatagaa cattctaacc
     3781 atagatattt tatagacccc cgacctgttg gatacgctac ccacctctgg atagttttcg
     3841 atagaagata tatgttcatc aactattacg gtatctttca gccgtcttta ccgagcttca
     3901 aacattctga gtttcctcat acaatgcttg tcggaggctc agggcaggcg tttcagagcg
     3961 ttacttcatc attccacctg tcagattatc agttctccta ataaccaaac tttttcgttc
     4021 ttttattagt gccttagctt ttcactaagg aacgagtcgc acaaaggttt tgtccaaaat
     4081 tcataaaaat gttctcctct ctatattcaa tgaatttgta tttgagttat ttttttgttg
     4141 ttatcacgtc ctgttctttt actgactgtt gcttcaaaat ctgcttgtgt cggtctgtca
     4201 gtttcgcatg gtcgagaatg tcttttacaa cctgcgtctg gttgatttca tcaagtttaa
     4261 tcgcaacctt taaggtcggg gcaacttgat gagatagcca gttcagcgtc ctttggaagg
     4321 agtaaggctc tggttttgtg gttagtttta atcgttcacg attgttccca ataaaccaag
     4381 cccattcttc attcagtttc caatcagaac gaggtttgga atcgtcttta tctacaaaac
     4441 ggatataccg attgataatt ttaaaggcgg tatgctctgg attgtcatag acgagtaaat
     4501 cacggactgc ataataggca cgctcatttt tcaatcgaat ctcaaaacgg ttttttactt
     4561 ctgcgtcttc aatgggaata tcatttttct tgtactgctc gtagtccttt tcatagatac
     4621 agaaataaac ttcactttgt aatgaaccga tatagagggt gtttcccata cattcctttt
     4681 cctctttgcg taccagttcg ccactgcgat agcttttaaa actgcggaag acggagatac
     4741 attcttcctg ttggcacttt tcagtgagta cagggatatt taaaatccct gtcttatcgt
     4801 taatggcaag gtcaaggcgt ttcatcacac cgccagccac caaaacgtcc ataaagaact
     4861 cataccagct tctttgttgt gccagaagat agctttcaaa ttgtctgcac ccacgacctt
     4921 tcaattccac cagaactcct ttgtccagtt catgggagca aaggacgaat atgtcgccta
     4981 aagcataatg ctctgaataa gaatagaaac catagtcctc atgaagaaaa taggacagtt
     5041 tcagttgtaa gatgttttcg accacctgct gtacgtctgt tgtcggaaag cgaattctta
     5101 cataatcaaa cagcatttca aggggagcgt cgggattgaa gcgttccaga gcttcccaaa
     5161 gggactgctg taaatcctct gatggcttga cttttcctgt ttcaatatcg cttagatact
     5221 gccttgtaat accagtcgca acagctaaac ggttttgaga tagtccataa gccaagcgtt
     5281 tttcttttaa atgctgtaac caagtttgtt cattcagtaa aaatccctcc aatcaaaaag
     5341 gcgtatgtca acttttaaag cccatttgac atacgctgaa attttgtaaa tcccttgtaa
     5401 ccaaaggatt ttctaatgtt tttttgactg tttcctgtcg atttgtaccc ccctgttaga
     5461 taccgagggg ttaagtgctg gcgtggctat tgccacacca gccagcaaga tcagtccaca
     5521 cctgcgactt ccgcttcgca cgtcgcctgc gtggactgtc tgctgttgga taacttttta
     5581 atttcctcca agaaatcata tccttttggt acaaggggag tataaaactc tgatatgaca
     5641 cttgttccta catcaacata gccacgacct ttgattcgct ttaagaagaa atccttttgt
     5701 acgtcactgc caaacatcat gccatagccc atttcagaca tacgacctaa agccactctg
     5761 aaattaaact gatcacggat tccgtcgcct aaatattttg cgtctggacg ttgacaagcc
     5821 agtattagaa agaagccagc ttgacgacct aacatgacaa tctgtttcag cttattcata
     5881 actgcggtgt tttcttttgt tcccagcatt tccatgaaag cgacgtattc atcaaagatt
     5941 aagaagtgtg ccgggagacc taagtaagca taatttttgc cagtcttata gttcttcatc
     6001 tgcttcattt cctcactacg tttcatcatt tcttcataga atgtttcaat gcaagaaagc
     6061 aagtcttctt ttctatagta gacatttgcc atcacagaac ctaagtccgc aaggtcagca
     6121 tttttcgggt caagaatata cagttttgaa tctgtatgaa gcaaggcttc aatcagtgtc
     6181 agtataaagt aagttttacc gccacctgta ccaccagcaa tcaacatatg agggagctta
     6241 tcatattccc accatacgtt tttcattaag cgaagtttac catctttagc ttctacttca
     6301 tcaatagaaa tacgactggc tatggtgtca tagagcaaag tatattccac ataggaatcc
     6361 tttaactctt tatccgtcag ctcacagtac aagccactct ctaatttctt ttccaagtgt
     6421 aagagttggt cttgatattt tcccagcgtg atttccaccc gtatctgtat caagccattt
     6481 ttaagtcgat aatacatttt agggaagtag gttatctttt cctttgtacg accagcacta
     6541 tctttaaaga aaccctctgt tttgacctgt tcagattcat accacttgtt ttcaagtatc
     6601 atctttgcca gtttttgacg gtggtaaagt tgtttaaccg tatcatagcg aacccgtttg
     6661 aatacaaacg ctaccagcaa gcagataaga attgcgacac tgaaactgat aattaaatag
     6721 ggaatgtcaa tcttatctgc ttgtgatagg ttaaaatcct gccagttgat ctgctggatt
     6781 gtcttcacat gaaacagtcc gacaaccagc aggaaaacag gcaggagtga cgctatcgta
     6841 aaatgaaaga ctaaatcttt accagatggg cgaatccttt taccacgctg tttcatgcga
     6901 aaaagtctcc tttctaccta gcgactattt gtcttgtgtc ggttctttct ttgcttgtgg
     6961 ttgagctttg aatgaactag aatcctttgt cagcacaata tcgtctgcct tgatatacca
     7021 gtcaacatct gctccttgat aggtggcagt agcaacggtg tccgcaatgg gattgataag
     7081 ttccacccgt gcgttataat caaactcttt caaaggcacg ctggcaggaa tacttacttg
     7141 aatcatgcgt ccttgtcctt tggattttaa gtcataggta cgttccttga tttcatctga
     7201 aaccgacccg tcttcatttt ggattctcac ttcacgacgt agagcagaga atttcaattc
     7261 tccaaaagtc gtgtctttat ctaatacaat gccatttgct aatctcatca tttttcctct
     7321 ctttctttat tcttttatca tgtcgtcagc atgtaaaagg taatttgtaa aaccacgagt
     7381 gccgattttg tagccctctg cggtaatacg tggattgact aacttcacac gttcctcaaa
     7441 gccgaaatgt ttttcgccag cttcagcagg aagcaccacc acaatatcat ctgctctttg
     7501 aacatcagaa tagagattat agcttcttga taagacagtt agccgtccgt tgattcttcg
     7561 ctgaacgact ttatcctcgc cagcaaattc taaattgccg aatgtttttt ccatgttggg
     7621 aatcacaaat ttaagttcca tatttttacc tatcctttct tttttattgg ctgaatgaat
     7681 gtttgatggt cttaaagagt ggggaacgac cttttgattc ttgatttttt gttttcataa
     7741 gttcacttcc tttcaaaatc gggtaaaaaa atagacacct cattttttga agtgtctacc
     7801 tattaaatat tcaaatttta ttggaagtat ctttatatct tcacttttca aggataaatc
     7861 gtcgtatcaa agctcattca taagtagtaa attagtagta aattgagtgg ttttgacctt
     7921 gataaagtgt gataagtcca gtttttatgc ggataactag atttttatgc tatttttctc
     7981 ttaaaaaata tcattatttg tggtatggag cccccgcgtt gatccggaag gcacggtaga
     8041 tttgctcgac gaggatcagg cgcatcaatt gatgtggata cgtcattttg ccaaaagaga
     8101 tctgagcatt gcttcttttc atgacggctt cgctgagtcc taaagaaccg ccgataacaa
     8161 aaacaaggtg gctttttcct tggataccta attggtcgat ttgtttagca aaagattcgc
     8221 ttgtgggatt tttcccttca attgctaagc catagacgta ttcttgttct ttgattttcg
     8281 ctaagatccg ttctccttct ttttctttta cttgtctcat ttgcgcttcg cttaaattct
     8341 ccggggcttt ttcgtcagga acttcgatca attcgatttt cgcataggca ttcagccgtt
     8401 tgacatactc attgatacct tggatcaagt atttttcttt taattttcct actgaaataa
     8461 ttttgatatt catgttttct ctcctttgat cttcacgctc actttgttta ttataatcaa
     8521 ttttttcgtc tatatgttta ttttgactat ttttgttaaa aagatttccc caacagttat
     8581 gcacaacatc cacaggttta tccacagaaa acagcgttgt tttaccttat ttcatcacat
     8641 tcattaggca aacatacgtt ttataagaat ttatccactt tatacacagg ctgtggatat
     8701 gttttatccc ttatttttcc aatcctatcc gcgtagtttt acacaatatc tgtggaaaaa
     8761 gaaaatcaaa tattttcatc tgaaaacaat gatttttcct aaagtttttc ataaaaagtt
     8821 caaagtctca aagtagacta aagtcaacaa ataagactat actaacagta gataaatgaa
     8881 tgtaattgga ggaggaacac atggatagaa aaaatgtgac accaaagatg aaaaaaaata
     8941 aaaataatag cctctggcgt aagttgggtc tggggcttgt tggagggatc gttggaggac
     9001 tactgacagc tggcattttt tatgcagcga tgggcactgg caacaatagt tctactgcta
     9061 caagcggcaa tcaaaattct gccggtgaaa cagttgtgga aaatgtcaaa gtcaatgtag
     9121 attctgatat caccaaagcg gtagataaag ttcaagacgc tgtcgtttct gttatcaact
     9181 tacaaagtca aaaccaatca agcggttttg gcggattatt cgggcagcaa gaagaaagta
     9241 gcagctcaga tgatagtaat ttggaagctt atagtgaagg aagcggcgta atctacaaaa
     9301 aagacgggaa tactgcttat gtagtcacaa acaatcacgt agtagatgga cagcaaggtt
     9361 tggaagtcat gatgaaagac ggtacgaagg taaaagctga attagttgga acagatgcct
     9421 attcagattt agcggtgttg aagatcaatt cagacaaagt cgaaacggtt gcctcttttg
     9481 gggactcaag tgctttgaag gttggtgaac ctgcgattgc gattggttcc ccattaggat
     9541 ctgaatatgc gaattctgtc acttcaggaa tcatttcttc cttgaaccga caagtaacaa
     9601 gtacgaatga atcgaacgaa acagtcaata tcaatgcgat ccaaacagat gcagcgatca
     9661 acccgggaaa ttctggtggt cctttagtga atatcgaagg acaagttatc ggtatcaact
     9721 caagtaagat cgcaagtact tctgaatcga cttctaatgt aagcgtagaa ggaatgggat
     9781 ttgccatccc aagtaacgat gtagtgaata tcatcaacca actggaaaaa gacggaaaag
     9841 tgacacgtcc agctctaggt atcacaatgg tcgacttatc tgccgtttct actcagcaac
     9901 aagaacaaat cttgaagatt ccttcatctg taactaacgg tgtcatcgtg acaaatgtcc
     9961 aaacagctac accggcagaa aaagctgggt tgaaacaata cgacgtcatt actaagatcg
    10021 atgacaaaga agtttcttct ggtgtagaac ttcaatctgc tttgtataag aaaaaagtgg
    10081 gagattctgt caaaatcact tactatgaag gctcgaaaga aaaaacaact actgttgaat
    10141 tgacgatcga tcaatcggca ttaaaacaaa atcaaaatag tgaaaattaa taaaaaagag
    10201 gttgtgacaa agacttgtca caacctcttt attttggaaa tacttctccg actacggcat
    10261 cctcaggcaa tactaagatt cctttttttt ctggagcatt tggcaaatgc aattcacgag
    10321 cagaacaaat cattccatag ctggctaccc ctcgcaaagt tcctggccag atcatcattc
    10381 catctggcat catggctcct ggttttgcaa cgacaacttt ttgtccagct tcgatatttg
    10441 gtgcaccgca aacaatttgc aatactgtcc cattgtccac ttctgtctca gtaatcgata
    10501 aatggtcaga gtcaggatgc gggacgcatg tttttacata cccgacaacg atcttcggtt
    10561 catgatcagc ttcaagctgt tcttcgaatc ctgcttcttt aatcaatcga ttcaatttcg
    10621 ctaattcttc atctgttaag cttacttggc cgttcccttc aattgttaga tgatcggaga
    10681 tatggaaaaa gttccaagca acaacttgac cttgttcatc tttgatccga gcaacattcc
    10741 atttacgttc gacagtgttt tctgttcctt tatcgtcggc tatgataacc atcaaagtgt
    10801 ctccgacgtg tgctttgtta taagcaaaaa tcatgttctt tcctccatta tcctttgctg
    10861 agatttatct taacaaaaaa ttgcattcac tgcatgagat ttttttcatg ctttatttcc
    10921 gaagaagctt cttctggaac accatttatt cgtaacaagg gactgtgaca agacttttgt
    10981 cacagtccct ttactgtttg attaaaaagg ttatacaagt tccacttgct taattactcg
    11041 gctgttcttc ttcgtcagaa acgatcaaga catcacaagg tgaatgacgt atgacgtagc
    11101 tggctacact tcctgtcata aagcgttcga ctgcatttag tccagactgc ccaaccatga
    11161 ttagatcgat tgcatatttt tttggcaatt tgactgccat ggattcacga acagagccgt
    11221 atgcaataat tccttctata tttgtgtaat caacagattt tccatattct ttacattctt
    11281 cgatcatttg cttggctgtt tcagtttgct gatcgactag attttgatca ataggagcat
    11341 agcccataaa attataaaat tgctgatcga ttacgtttgc tacatatact ttcccattat
    11401 tacggcgagc cacttctaac gcttttttga aagcagcaag tgcttgttca ctaccgtcaa
    11461 tacctactaa aatgttctta tattcttgat tcatcttgac ccctcctaac agatgtcagc
    11521 cttttattac actttctagg aacgattcga tttcctgttt tgttttacga tctttattca
    11581 ctagacgacc taattctttt ccgtcttgga tcacgacaaa acttgggata ccaaaaatgt
    11641 tccattcagc ggctacatct atgtacttgt cacggtctac ttcaataaat tgccaatcag
    11701 aaaagtcatt ttcgatttca ggcatttgcg gtttgataaa acggcagtct ccgcaccagc
    11761 cggcagtaaa gaaaaataca ctttttccag tttctacgta gcctgctaat tcttctaaac
    11821 tatttgggat aatcattctt tttcctctac tttctgttta tgtatttttc aattaaatta
    11881 tacacctttc tttcgaacta acaaacattg atcctctttt tataaaaaaa atctgacaaa
    11941 aggttgcttt tgccagattt tatagtttca ctatcgcgtt atttcccgta tacgatcgtg
    12001 ttcactgttg atttatctaa acctttcaat gtttgaatca acatttctcg tgcagcttca
    12061 aaatcgcgaa tactgaacat cgtttgatgc gtatggatat aacgtcccac tacgccaatc
    12121 actgtacttg ggattccttc attttgtgta tgagcagcac ctgcatctgt accgcctttt
    12181 gaaacaaagt attgataagg gatgttgttt gtttcagcga tatctagcaa gtactcacgc
    12241 aaacgaggaa gcatgatcaa acctggatct tggatacgca tcaatgtacc ttctcctaaa
    12301 tgtccgaatg tgccattttt cgtcactgtg tcatctgctg cagagcagtc tactgcaaag
    12361 aacagatctg gcttgaattt attgacagaa gctttagaac cacgcaagcc tacttcttct
    12421 tggacattcg cgcctgcaat caatgtatga cctaatgttt cattttgtaa cgcttctaaa
    12481 gcttctaata ccattgtaca gccatagcgg ttgtcccacg atttgctgat gatattcttc
    12541 ccgttggctg ttttgatcgt ttcagcgtaa ggaacgattg tgtctcctgg aagaacaccg
    12601 taactcattg cctcttctct tgattcaaac ccagcatcga acaaaatatc tgttacttct
    12661 actgattttt gtccgcttgt tcctcgtaat aaatgtgggg gaacggaaga agaaatacat
    12721 ggataattct taccagtaga ggttttaagt gtaaaacgtt gagcagaaac aacgtaaggg
    12781 ttccatccgc ctagaggtac gactttaaaa agcccgtttt cttggatttg tgtcagcatg
    12841 aatccgactt catccatatg tgctgcgacc attacacgag gtgcatccgc ttcttcatgg
    12901 tggcgcagcc caaaaatgcc gcccaaacca tctaactgta actcatctac cagcggttcc
    12961 atatgacttt ccatataggc gcgaacgtct tgttcaaaac cgcttgttcc ttgtaattct
    13021 gttaactctt tgattcgttg aaatgttttt tcttccatat gctattcgtc taaatttcag
    13081 actaaactcc tctcagactt atattgatct gcggataaaa tatgattttt tctgcagacc
    13141 gatcgtattt attatagctt attttttctg gtagcggtta tttatttcaa agaaaatatg
    13201 agagtgtggt aaaataaaca caaagagtag caagtgagaa gaaggaggta tatccacatg
    13261 tatgaagaaa gcgaattaag ctattttaaa ggcgggttag ccgttgggat cggtctagga
    13321 ttattgagtg gggtagcttc aacgatttgg tatcatcgca aaaaagtgtt atcagcagac
    13381 caagtgcttg aaaatatcaa aacatctttt ttaaaagaag gcgcaatcga aggtgcttgg
    13441 atctcttttg aaaaagaacc tattcggaaa ttcgccgttc ggtcgagtgc ttatcggggt
    13501 gggctttccc ggatagaaga tggcgaaact gtttattatg aatttttagc agatgctcat
    13561 acaggaaccg tattagacat ttcaagaaaa aagggcgcaa tagattaata cgtcaaaaaa
    13621 atgaaataac gaatacgcac ccactatcta aaagagaggt atcctatcct tcttttatag
    13681 tgggtgattt ttataaaaat agggctattt cctcttggaa tagctagtat ttgtagttat
    13741 tatacggtat tcttgaaata agattagttt tattatataa ggagagtgac aaatggaatc
    13801 acgtgaaaaa attgccgtgc tcattccttg ttataacgaa gaggcgacaa tttcaactgt
    13861 tattgaagat tttaaaaggg aattaccaga agcagatatt tatgtctatg ataataattc
    13921 gaccgatcga acatacgaat tggccgttca aggcggagca atcgtaaaga aagaaccacg
    13981 tcaaggaaaa gggaatgtaa tccgtcaaat gttttttgat attgatgccg attattattt
    14041 gatggtagat ggtgatgata cttatcctgc agaagctgca catggtttgc ttgagaaatt
    14101 acgttctggc gaagcagata tggtcattgg cgatcgtcta tcaaatggaa cctattttga
    14161 tgaaaataag cgtcctttcc atgattttgg gaataatctg gttaaaaata caattactta
    14221 cttatataaa acaaaaattc gcgatgtcat gaccggctat cgaggattca atcgaatttt
    14281 tgtcaaaagc ttccctatta tgagttctgg ttttcaaatc gaaacagaat tgacgattca
    14341 tgctttagat aaaaaattca agttcgttga gattccaatc gattatcgcg atcgtcctga
    14401 aggtagcgaa tcaaaattga atacattctc tgacggattc aaagtgatca tgatgatcat
    14461 caaaatgtgc aaagattata aacctttgct tttctttggg atctggacca tcttcttctt
    14521 tttatttgga ttggtcatgg ggattcctgt gattcgtgaa tatatgctga caagctttat
    14581 tacacgcatc ccttctgcga ttttaagtac tggattgatg actttagccc ttctttcatt
    14641 agtaacaggg ttgattttgg atacggtcgt cacaaatgcc aagaaagaat atgaattgaa
    14701 tttataccat gtctaccacg aatatcaaaa taaagaaatg aagtaggagg ccttttttat
    14761 gcataagaaa aaggagaatc tatttcctac tttttttgca actttatttg cgtcaggttt
    14821 tgcttttttt acttcttatg gtctaggaac tttgaatcca acagtaatca ttggtttagc
    14881 catggttttg ctacttgctt ttaagcaata tggaatagag ttgttttcaa taaaaaacaa
    14941 agttgcagct attgcccgat tgtttttttc tgcagctttt gctcttacac tttctattgg
    15001 cagacagata caatttcagt atgtctcagc aaactataca aagacaacga ttgctgatgg
    15061 aagtattttt tactttcttg tttatttctc catttttgtg tattcttttt tatttttaca
    15121 atcgatttat ttatcaatgc caaaactgga aacatttttt actgctcaaa acacacaggc
    15181 tttctcttca agaaaatatt ttttattctt tactgtcttt ttagttctgt gttggcttcc
    15241 ttatctgttg gcattgtatc ctggagtggt tttgcctgat tcgttaagtt cggtcgccca
    15301 atctatgggt gatgttccta taagcaatca tcatccagtc ttattcacct tactggtcaa
    15361 gctatttgtt catgatttac caactgatac gatcaatcac ggtgtatttt tattttcgat
    15421 ttttcaaaca ttgataacag cttcagcgat aagttattct ctttgctggc tgttaaaaag
    15481 aaacatccat ttttttccgg tactcattag tttatgctat tttgcatttg caccggtttt
    15541 tccaatctac gccttgaaca tgcaaaaaga tactttgttc tctatcgctt gctttttatt
    15601 ttccttagcg gtttttgact ttatttttca acgagaaaag ccaacgattc tcaatcagat
    15661 ttacgtaatc atccttgctt tactgaccac ctatctaaga aataatggga tttatgtagt
    15721 aaccggcaca ggtttgattt gcggcttgat tattttgaaa caaaaaaggt atcaagcatt
    15781 ctttcaagtg gtgctgggtt atacagtgat tgctggacta ctcatccacc cgttgatggc
    15841 tcgttatgct attcctacag aaactgctga acaattaggt gttcctttgc agcaaatttc
    15901 tagaactatt gttatggatg gtgacttgtc taagaaagat aaagcattca tgaatcagct
    15961 actcccgata aaagattatg aagcgtattc tcctagtctt gcagactcaa tcaagtggca
    16021 taaaaatttt aacaatgagt ttttgaatac acataaaaaa gaatttctat cactatgggc
    16081 aagaaattta ccaagtaata taaaaatcta tacagatgct tatattttag aaacgttcgg
    16141 gttctgggta cctggtacaa aaaatagtta cggcttttta gatactcgtg taaacacgaa
    16201 taattatggt atgaagcaag tagatttgat tgaacatctt actggattgt caggaatgaa
    16261 aaaaatcatc gatgctcgtg atttctttgg ttccggcaca ttactgtggt tagctatcgg
    16321 cagtgcactt ttagcaatct ttaagaaaaa atatcggtat ttactccttt ttcttcccgg
    16381 tatgcttgtt tttgctacta ttatgattgc tactcctgtg gcttttagtt tacggtatgt
    16441 gtttgtttta gcattaggtc tacctgttta tatcctctct ccttttttac tagataaaag
    16501 agaaaaaatg aaaacagata taggagatac tcatgaaact atttaaaaag atattttttg
    16561 cttttgttct cttgatcgct agctattgcg tactactgac aattactttt ttcattccgc
    16621 agcactctat tgaatcgaat gcagaaaaat ctctacagat ggttgaaaaa gaaggaatgt
    16681 atccttctat caatcaagga aatatgctag gcactcggtt agataatttt actgatcatt
    16741 tgatgatacg aaaaacaaaa gcagatcctg aattgaactc cttggagaac gcgatgtcta
    16801 tggcggatta tccccgttat tggcatggtt atcagctttt tttacgacct ttactgctag
    16861 ggatgagttt ggggagtatt cgaatgattt acgcagccat tctttttctg ctgattggat
    16921 tgacgagcta ctttttaacc aagtgtagcg acatctattt aacgacagcc ttgttgattt
    16981 cgcttgctat aggaaatgct gcaacctttt tcttcagtat gcaattttca aatatttgga
    17041 ttttgacttt attgtctgta ttactatttt taacaaaacc acaactctcc aacaccaacc
    17101 aacaattatt cttgtatttc tttgtcattg gttctttagc taactttttt gacttgttga
    17161 caacaccggt catctcttgg gggatacctg tgatcattat ttattatgta acaaataagt
    17221 atttaatgga taaacgcagt tcattgagca aacaagtagg cagttttgtt tttaccggta
    17281 tcttttgggg attaggctat ggtttgacat gggtggctaa atggatccta tcatcgatca
    17341 tattgaacaa aaacattgta aaagatgcaa tcaaccaaat cctctttcga acggaaggaa
    17401 acgacaaata cccgttgcat cgattagaga tgctgaaaag caatattaga ttgatgtatc
    17461 caaaagtagc aatattattg ctattgatta cttgtcttgt ttttctctat tttgcttatc
    17521 gtaaacgaaa tagctggcgt ccgttccaac ttctagtttt gtttgtttta gctgctgtaa
    17581 cgccgtatat ttggtacaat attttagcta atcactcaca gattcattat tggtttacgt
    17641 atcgtacgca aatcatcaca agttttgcta tacttagtgc tttcgccttt ttgatccctc
    17701 ctgagaaatc aaaagacgaa ctaaattact tttaagaata ccgatgtact aattcatttt
    17761 taaacataaa aactccagct gtcagagata gccg
//
LOCUS       DAZKCZ010000049        17340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000049 DAZKCZ010000000
VERSION     DAZKCZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.049"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(118..663)
                     /locus_tag="ACG8KN_001534"
     CDS             complement(118..663)
                     /locus_tag="ACG8KN_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9188594.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(675..1367)
                     /locus_tag="ACG8KN_001535"
     CDS             complement(675..1367)
                     /locus_tag="ACG8KN_001535"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9188595.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1386..1670)
                     /gene="macP"
                     /locus_tag="ACG8KN_001536"
     CDS             complement(1386..1670)
                     /gene="macP"
                     /locus_tag="ACG8KN_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFP9188596.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1683..2246)
                     /locus_tag="ACG8KN_001537"
     CDS             complement(1683..2246)
                     /locus_tag="ACG8KN_001537"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9188597.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2474..3136
                     /locus_tag="ACG8KN_001538"
     CDS             2474..3136
                     /locus_tag="ACG8KN_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFP9188598.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3260..4456
                     /locus_tag="ACG8KN_001539"
     CDS             3260..4456
                     /locus_tag="ACG8KN_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFP9188599.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4557..5933)
                     /locus_tag="ACG8KN_001540"
     CDS             complement(4557..5933)
                     /locus_tag="ACG8KN_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9188600.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5970..6836)
                     /locus_tag="ACG8KN_001541"
     CDS             complement(5970..6836)
                     /locus_tag="ACG8KN_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9188601.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6925..7950)
                     /locus_tag="ACG8KN_001542"
     CDS             complement(6925..7950)
                     /locus_tag="ACG8KN_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9188602.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7968..8642)
                     /locus_tag="ACG8KN_001543"
     CDS             complement(7968..8642)
                     /locus_tag="ACG8KN_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9188603.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIKGYIETKVG
                     QGYIVP"
     gene            complement(8826..9719)
                     /locus_tag="ACG8KN_001544"
     CDS             complement(8826..9719)
                     /locus_tag="ACG8KN_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9188604.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9793..11490)
                     /locus_tag="ACG8KN_001545"
     CDS             complement(9793..11490)
                     /locus_tag="ACG8KN_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9188605.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11483..12796)
                     /locus_tag="ACG8KN_001546"
     CDS             complement(11483..12796)
                     /locus_tag="ACG8KN_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9188606.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12796..16737)
                     /locus_tag="ACG8KN_001547"
     CDS             complement(12796..16737)
                     /locus_tag="ACG8KN_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9188607.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFHHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16846..17304)
                     /locus_tag="ACG8KN_001548"
     CDS             complement(16846..17304)
                     /locus_tag="ACG8KN_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9188608.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4858 a         3889 c         3020 g         5573 t
ORIGIN      
        1 aaaaagaata agttttttta aaataacgta taaaaaagag cataaaacaa agtcgtttgt
       61 tttatgctct tcttttatta ttttgtgctt tttattttat caaatcactt tgttttttta
      121 taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat gtccaacggg
      181 atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat tatacgcatc
      241 gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa tatctgtgat
      301 ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat aatgccgttt
      361 gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt ttaaagagcc
      421 gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc ggttctccgg
      481 atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat ccttttgatc
      541 aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg ctgtcccagt
      601 agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt
      661 catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc tgctgatcgt
      721 ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg
      781 ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg tccaacagcg
      841 gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc tttgatttta
      901 tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc gttcaaattc
      961 gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc ataatataga
     1021 ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc atcaaaataa
     1081 gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat tccgccagct
     1141 gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact tgtggtaata
     1201 gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt acctaatgaa
     1261 cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag gttctctctc
     1321 aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt tttaattcct
     1381 tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat aacaataata
     1441 agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct gatccttgtt
     1501 ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat ctttttttct
     1561 tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct tgcttgtgct
     1621 tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat aagccgatcc
     1681 ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt ctttgcatca
     1741 caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg atacaactcc
     1801 agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc ttgtgcatga
     1861 taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt aagatggctc
     1921 agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt catttcggga
     1981 ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac tttttctaaa
     2041 ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg
     2101 acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg caaacgaact
     2161 tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact gattgttttt
     2221 tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat cataactgct
     2281 tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata gataaacctt
     2341 agattttctc taaatataga aaatccttct ttagtccgat actctttcta gtagtccgta
     2401 tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg ataaatgaca
     2461 tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg tcttgctatt
     2521 tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga taggtgtagt
     2581 tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc
     2641 tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat
     2701 ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa
     2761 tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag tcaaagcagc
     2821 gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag caagtcagtt
     2881 tctttatacg catctgccga atctagttga tttgacaaat aagtataatg agatcaatga
     2941 ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc agatcatcga
     3001 ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg atctagatga
     3061 tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata aatatgaacc
     3121 agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc agatgatgtc
     3181 tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg agaacagatt
     3241 caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg ttacaccagt
     3301 taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag atacgctgac
     3361 cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg ctcgtttaat
     3421 tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa aaatcgatgc
     3481 taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat taagtgaatt
     3541 ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag gagattcctt
     3601 aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg ccggagaagg
     3661 gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag tgacagcaaa
     3721 atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata aagaaaaaga
     3781 cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca aagactactt
     3841 tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac ttcaaacaac
     3901 gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg atgttcagat
     3961 tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg atcttcgctt
     4021 agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa acacaaatca
     4081 ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt ggaaaaacca
     4141 agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc aacgccaagt
     4201 ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa tctcagccat
     4261 cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc aaaccactca
     4321 aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag gaaaagaaaa
     4381 acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac aaaaactgtt
     4441 ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca acaacagttt
     4501 attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat tggagtctat
     4561 ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag tccaataacc
     4621 attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa
     4681 gtcaagatgg agacgattgg caatagcgga acgaatggca ctttgaattc tccagctttt
     4741 ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat caaataggcg
     4801 agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat
     4861 acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt ttgatcgatt
     4921 tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa acgagcaagt
     4981 gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac agcgatcacc
     5041 gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc attgactcct
     5101 agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta aaggacagta
     5161 gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt tttgacttcc
     5221 tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag agctgcacca
     5281 tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg tttaacgaaa
     5341 aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac catggcatta
     5401 ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa tatcactacg
     5461 agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc agttaatgct
     5521 tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga ccatccggaa
     5581 gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt cagccaggca
     5641 gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac tggcactcgc
     5701 gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc taagacaaat
     5761 gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac cacaaatatt
     5821 cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt tagttctttt
     5881 ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc cattgaagtt
     5941 cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc taaaaggata
     6001 ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt tttcgttttt
     6061 ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat ttgtgaaagt
     6121 gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat gtttcctaag
     6181 ccccataata atccacagat catgtttttc cacacaaact gatcgacttt aactttacgg
     6241 aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat aattgacatc
     6301 gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat cgtagagaag
     6361 atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc tatttttcct
     6421 tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa agcaataata
     6481 ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta
     6541 ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc catgtacttc
     6601 attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa aaatccgatc
     6661 aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa agaaaacaga
     6721 aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat tttaccactt
     6781 actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat ttccataacc
     6841 aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac aacacaagca
     6901 ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta agattgggaa
     6961 aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat ggatacctaa
     7021 tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg ttttgctaag
     7081 tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt ccgctgggat
     7141 accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt ctatgctcac
     7201 aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt ggttgaagat
     7261 aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg
     7321 aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa tgattccttt
     7381 tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg cataatacaa
     7441 aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg tatacttttg
     7501 atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa gtggcacttt
     7561 gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac tgattgctga
     7621 ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta ccagtttttc
     7681 ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc
     7741 taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa
     7801 gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga tgtttggcgc
     7861 cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca
     7921 ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca gggaacgata
     7981 tatccctgtc ctacttttgt ttcgatatat cctttgatac ctgcctgttc gatttttcgg
     8041 cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac
     8101 agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat caaaataaat
     8161 aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc catacttccg
     8221 ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa ttctgtgtag
     8281 ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat ttggaaatgt
     8341 tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat attcgtattc
     8401 ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg acaccaataa
     8461 tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc ttctctttca
     8521 aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg ccatttctca
     8581 agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat gatttttgcc
     8641 ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc cttgtgaaac
     8701 aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg
     8761 atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc ttaataattg
     8821 gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg ataacaccaa
     8881 taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa tcgataaaga
     8941 tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg aactcgtctt
     9001 cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact
     9061 agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac aacaattttc
     9121 tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg gtcatcttgc
     9181 tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg ggcggtaatc
     9241 acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac tgtcgctcct
     9301 ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt gattttcgga
     9361 atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt ttggacattt
     9421 ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac tgatgtatga
     9481 gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt ttcttcttta
     9541 tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata attgctgaga
     9601 gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat cactgccata
     9661 aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt ctttttcatt
     9721 ttcttcacct tcttatttaa aaaaactgca gaaaccttta taaaaccgat tgctgcagct
     9781 tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc tgtacgctcc
     9841 aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac cagtagcagg
     9901 aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta ctcctacgat
     9961 ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg ctgcctcacc
    10021 tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg ttccataatc
    10081 taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg cttgtccgct
    10141 tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg actccccttt
    10201 ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg catctttaag
    10261 caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg tgactttctc
    10321 tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt caataaccgc
    10381 tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat tatacgtgat
    10441 cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt tggctactcc
    10501 attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta ctatataatc
    10561 ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat cataataagt
    10621 tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc ttccgttttc
    10681 aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc
    10741 tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa taatcgttac
    10801 ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga tttcgttttt
    10861 cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat cccacacgcc
    10921 atccaccata tcaggcatca tcactggcat cataacaacc attggagacg cgattctgga
    10981 aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag aagaaggtgc
    11041 ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg ttgtttgttt
    11101 gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt tttgaatcca
    11161 ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag gattatctcg
    11221 aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc ctggaatatt
    11281 ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg gaacagatga
    11341 gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga cttcttcggc
    11401 tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta acagtacact
    11461 cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt tttattaata
    11521 aaacagcacc taacaataaa agaattccta gtaaagaaat caacgttctt gcttcattcg
    11581 tttgcgggaa tacggaccat tttcctgatt gacctggatt gcttggacca ctcggctctt
    11641 tcggtttgtc cgttgtttct tttacggtct cgttataaac acgtggtgtt gcggattcat
    11701 aggcagagac tggaactttc ccggattcta attcagccat ctcctgtccg ttaactacta
    11761 catattgtgg ttggcctttt gcatccaccc aaaattcagg gatgatgacc tcgattctct
    11821 tacttgcttc atccatctcg taaccttcca caccttttaa ttcttcaaaa taataggtgc
    11881 cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa atgaatttgc
    11941 ttacattttt gtcattcaaa ggatcattgt ctgctggttt gatccattga tttttcaagt
    12001 cattagcagg agccatatct aaataatatt tccccccttc tatcatctga tatagagcaa
    12061 aaacagcacc ttctaatggt tttccttttt cagttgttcc tctttgacgt ccatacttga
    12121 agaagtatgg atctcttaga taaccgacat ttttaggata gatatgaatc tcttgcagtt
    12181 cctcggttga ttctgtcggg tgataaattg gcaaaatagc agcaattggg atagctgtct
    12241 gatcgatatc aacatccaat ccaacttctg gatcaatcga ttcttcgaaa atcaaataaa
    12301 ctgcatcccg atcttggttt ttcttaggtg ctttgatacg tgcaatcccg tctcctaatc
    12361 cgccaaccgt atctttccca gttgttaccg aagtccctat ttggacaagc ctttcttctg
    12421 tgattagctg acggatattg tttctgttcg tttcagcgat ttgctggacg aattctttag
    12481 gagtcatgtt ttcagcagct tttttttcat gataataatc ggtcatgtcg taaatggcaa
    12541 atgtcgctcc atttagtggc accgtctgtg aaatgatatc tgctgtttcc cctgcttcct
    12601 cagagatagc caagccatca ttttcatggt attcaaattg atcgttataa tcgatatctc
    12661 gaatcatccg tttatgcaac acgaattcca cgacatcttc ccctgattct tctgccgaca
    12721 tcgcatgtgg aagaaggact gctgcaagcg gtagtaaaac aagtaattga agcatcaaac
    12781 acaacagctt tttcattacc ttgctccctc ctttttagat tgtctgacaa acaaaccata
    12841 ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg atatagaata atcgtccgga
    12901 tcctcctgta gcaggcaacg gattttcagg atcgttcccg acactaagct gtaatctgat
    12961 tcgatttgtc tccaaaagtt catattctaa ctcttgctgt agatcaggcg tctctgcttc
    13021 ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc gcttcttttc ctggaactac
    13081 ccgatcattt tctatgtcag gttcttctcg ataactaatt tctaaaataa agtaacctgg
    13141 aagttttcta tagctgtcag gtgacttcac ttccttgatc cgatagaccc cttccaacaa
    13201 gtctgaagca ttgaataggg aagaatcgtt tggatctggt gccaagcctc cctcgatgat
    13261 ttcatactct cctgcactat tttgtttctc aattgaaaat tctgcgcctt caagacgtcg
    13321 gctattcata ctgtcaattt tcaaaagttc caaatcgatt ggcgctagtt catcttctaa
    13381 ggttacttgt agtacaccat cgatgatttc atagccttcc ggcaagggag cagtttcgtc
    13441 ttcgatgacc gtatcttcaa tgataaactt accttcttca ttcagtgcaa actggaacac
    13501 ttgatcagat agccgatacc catttggtgc gacagattcc gagacctcat attcaccaat
    13561 agacagatgc agaggttggt cgtaatttaa atcgacatac ttttctactc ctacggcacg
    13621 agaatagaag tatccgatcc caggcaaacg tttcgattct tctggttcta acggattacc
    13681 agtgtatgct cgatacagcg ggaaggaaga atcccccact ttcctgatat cgaaacctgc
    13741 accatctaat gtatggcctt catggtctaa ttttctaact cggatctccc gataatcaaa
    13801 cgtattgtaa gcagtgaatg ctacccataa acgatcctct tcatctgttg tatacgtgag
    13861 atattgccaa ttttcttcac ctgtttgctt gagttggata gtccctatag cattatcgta
    13921 aagtcgcaag atccatgtgt cattttcatc aggttctttg aaaccttctg ggttctggct
    13981 ctcaatgatt ttgtattctg catagttggt agtttcttca ctatccttat aaacaaatga
    14041 atctggctct ccgattggat gaataacttt ctcaccatcc acgattacat atttcccgtc
    14101 ttcatctact gaatagaacg tgagtctccc tttctcttcc tctacttcgt tcacaccaga
    14161 aagccctttc gctactggat atccgatttc cagtccgtta ccatctgttt cataaagttc
    14221 aaactctgcg ccattgattg gcatattact gccttcaatt agtttattga tattcatgtg
    14281 gattggcttg aattcatttc gcatttccag tttgacatac aactcatcat actctgtgcc
    14341 ttctatgtta ggctcttgta caacagaaag cttgtgatat tctccttcat aataggaatt
    14401 cggatcttta ccttccggtc cgccgactct cgaaccagat gtatattcat ctacaagctc
    14461 gtagtgacct aacatctcat cttcgttggc tgcatatttt ttgatttgga attgatacgt
    14521 ctcatctact aactgataac gtggtggtcc agcgatctct ctgatccaaa atatgtcgct
    14581 gactgctcca gtttcatctc ctaaaatcgg catcttcgtt tcaaagatcg ctattccgtc
    14641 cgtggtagtt ttacttgttt cgattggttc ggcttcccca ctctctggat tttggtcata
    14701 taatgcaaat tcgactccag ccaacggctc gccattttga cccttcttgc tgacttggaa
    14761 attacggcct ttatgttcgt aacgaatgga agaagatggg aaatacatcc gtccagtagt
    14821 gatatcattc tcataatcct ccaggttctg aatataggcc atattgttca ctgtgcaaaa
    14881 atttccgtct tgagctcccg catcaagttt tacttggtac ttgatcctga ccatttcccc
    14941 ttcacctaaa gaaatcggag aaaatttata agcttggttt tcccgtccga catacgttgt
    15001 cggagtcgcc actacttctg gtggggcttg tccatgacgt tcttcatccc atatttctgg
    15061 ctcttggtac ttcatgccgt tttctcctgg atctagatgg taaaactgaa tatctgccga
    15121 tctgccagct ccaaatggtg agacattaaa accagtttca tcataaagtg tggcttgctg
    15181 gatcgttctc ttgaaggtag aagagatatc ctccaaggct gtaacaatgt cattcttgtt
    15241 gttcgcattg taatatcgcg gtgttacgcc atcttcttct aaatcagcaa tatttttcaa
    15301 tacatttcgt ccaatagcat ctagtcgttg tctcccgcta acgtttccat cgattcctaa
    15361 accaatggag aagatacttg gcgggttctc ttgatacttg tttttcagcc aatgatgata
    15421 agcaactgtg ttgatcgcag atgacttttc ttgcgaagta ggactgccat tcgtattgtt
    15481 attagaagaa ccatcaccaa cccagtgagt cgctttatta tctccttctt ttacctctcc
    15541 ataacgatag tgtacattta cttcatcttc tggacggtta aagtcagatg cataggtaac
    15601 tggatgagta aaccctgcat tagaagtatt cccagaaaac tcctcaaaat ttctgaacaa
    15661 aacgtcatta tcttcagcaa tcttatcttc catcacagtc cagttgctcc aagctccatt
    15721 agtgctcgta cgataacttg actgaatcgg tgcataactg aaggtcggtg tcccatctcc
    15781 cacaacgatt aggatttttt ctggatttct attttctccg ccattgtctt tatacaatgt
    15841 ttcatagcca tttttaagcc ctagtgtaag cggagttcct ccgattggtg tacgatttaa
    15901 tgctgtattc tcacttcccg tcaaacgatt gatatcattt gtcaatggaa agatgtccga
    15961 actgatttgc tcatggttat tgatagctgt actattatgg tgaaaattca ccataccgat
    16021 tcgaatatcc agactcgggt cagagagcag atctttactc atttcgttta ctgcctcaat
    16081 caacgcagca tttttagttt gactattggt cccagcggtt aattcactca tactcgctga
    16141 cttatctaga acaaaaacga tatcgattgg actgatttcc tttttctcgc ccccgatgac
    16201 atctagatag acattgtaaa gggttgttgc atcttcagga tgtagatcag atgctgtgct
    16261 gcttggcaga acaggttcta cccatttttt gatgtacgcc ccgttataat ccaagtaact
    16321 gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt tgagctggaa cgtttctatc
    16381 ttcttcgcct gcagttgatc cattattagt agcatagtcg taatttcgcc agttcgctcc
    16441 cccgtttgta taatcactat catcgatcaa gttctctccg tcatatggcg agcctttgtc
    16501 tccttgatag cgttcattgc tttgtgtcca atttcctaac acttttgtat agtcattcgt
    16561 tgtaaacttc gggtagcgat gctgttcgtt ttcaacttct ccgctaggat agatatattc
    16621 ataggggtct tgcgtagctg ttggcccaag acgctccaaa ataccgttga ataactggcg
    16681 ttgaatgcgc tggagtgctt gaccgctggg tacgattggt tcgtccagct tttccatctc
    16741 attttcttcg ctacgttgaa tttcagcgga atcttctgta tcagtttcat tttcttgtaa
    16801 ttcttggtta tctattcttt catcaaccac cacttgaaga ggtagttacc cttcgttcac
    16861 atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt ttgaataaac
    16921 ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt ctccatcttc
    16981 atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc cggcggctgc
    17041 cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt caaaagccag
    17101 atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg catagtcctc
    17161 atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat ccagggtcgt
    17221 catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta ctaactgcaa
    17281 aagcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt tgtcttcttt
//
LOCUS       DAZKCZ010000050        16741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000050 DAZKCZ010000000
VERSION     DAZKCZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.050"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..845)
                     /locus_tag="ACG8KN_001549"
     CDS             complement(36..845)
                     /locus_tag="ACG8KN_001549"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9188609.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1138..2574
                     /locus_tag="ACG8KN_001550"
     CDS             1138..2574
                     /locus_tag="ACG8KN_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9188610.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2744..3940
                     /locus_tag="ACG8KN_001551"
     CDS             2744..3940
                     /locus_tag="ACG8KN_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9188611.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4002..4187)
                     /locus_tag="ACG8KN_001552"
     CDS             complement(4002..4187)
                     /locus_tag="ACG8KN_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFP9188612.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4418..7732
                     /gene="dnaE"
                     /locus_tag="ACG8KN_001553"
     CDS             4418..7732
                     /gene="dnaE"
                     /locus_tag="ACG8KN_001553"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9188613.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLGPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            7900..8862
                     /gene="pfkA"
                     /locus_tag="ACG8KN_001554"
     CDS             7900..8862
                     /gene="pfkA"
                     /locus_tag="ACG8KN_001554"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9188614.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8936..10720
                     /gene="pyk"
                     /locus_tag="ACG8KN_001555"
     CDS             8936..10720
                     /gene="pyk"
                     /locus_tag="ACG8KN_001555"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9188615.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10755..11657)
                     /locus_tag="ACG8KN_001556"
     CDS             complement(10755..11657)
                     /locus_tag="ACG8KN_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9188616.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11843..12412
                     /locus_tag="ACG8KN_001557"
     CDS             11843..12412
                     /locus_tag="ACG8KN_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFP9188617.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12470..12649
                     /gene="rpmF"
                     /locus_tag="ACG8KN_001558"
     CDS             12470..12649
                     /gene="rpmF"
                     /locus_tag="ACG8KN_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9188618.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12819..14240
                     /gene="gndA"
                     /locus_tag="ACG8KN_001559"
     CDS             12819..14240
                     /gene="gndA"
                     /locus_tag="ACG8KN_001559"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9188619.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14433..15119
                     /locus_tag="ACG8KN_001560"
     CDS             14433..15119
                     /locus_tag="ACG8KN_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9188620.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15116..16621
                     /locus_tag="ACG8KN_001561"
     CDS             15116..16621
                     /locus_tag="ACG8KN_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9188621.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5376 a         2941 c         3524 g         4900 t
ORIGIN      
        1 gtcacaacct cttttttgtg gaatctaatt ctctattata gtttaagtag tttttccaga
       61 taaacagcta aaccgtcttc ctgattgctc aaaggagtga catcatttgc aatgcctttg
      121 agttggtcag taccgttagc catagctact ccccaacctg cataatccaa caattccaaa
      181 tcattatgct cgtcaccaaa ggcaatgacg tctttttgat cgatagacag gtagttagca
      241 atctcttgca cgccttttgc tttttggatt ccttttgaaa caatctctaa gatggcagta
      301 ggtccacccc atgtccgaac atcgacataa ctgccaaatt gacgtgttaa ttcagcagat
      361 acagctcctg caaaacgttt gtctgttcgg acaagcaaag atgtaggatt tgtcgtcaaa
      421 tttttaggag aaagcaaatt cttctctcct ggacgagaag aagcaaaaat tttttcatca
      481 aagaaatcaa aactgtcaat aaaaaaagta tcacgatttt cagcagcgat aaaatctaaa
      541 tttagctgct ttttttgcga taagatttca aaagcgattt cacgattgat caacgtttct
      601 ctttcaccat cccagtgctg attagggatg tgtactaatg cgccgttaaa attaaccatc
      661 ggtgttgtta attctaattg ttggtaaaac tgatggctca ttcggaatgg gcgtcctgta
      721 gcaatactga catgatgacc ggcattgatt gcttttttta gagtctcttt tgttttagat
      781 gtaatcatcg aatcttgatt gagggtagtt ccgtccagat cgatggcaat taatttttta
      841 ttcaataagg gtccttcctt cctcaactag catttgtttt agtctagcat attttaggta
      901 attaacaaga gcaatcagct attcctaaca aaaactacag ttatggctat ataaatagct
      961 aaacataagc taaacacgaa cgttaagtat tattttcgct ttttttgtta aaaaaacgtt
     1021 gaaaagcgaa tgaaacgtct atataatttg aaattgttcg atggatttgc cctattttac
     1081 ttatttttta ttattatagt tttccatcag aaaaaaattc agggagtgga actttttatg
     1141 gaaaagtttt tcggtttaaa gaaaaacaat acaaatgtat caactgaaat aatggcggga
     1201 gtaacgacat tctttgccat gagttatatc ttattcgtaa atccgacgat tttgtcagca
     1261 tcaggtatgc ccttccaagc agtatttcta gcgacaatca ttgcatcgat tattggtacg
     1321 ctggtaatgg gattgttcgc aaatgtacca tatgctcagg cacctggtat gggattgaac
     1381 gcatttttca cttttacggt cgtttttggg cttggttatt cttggcaaca agccttggca
     1441 atggtgttta tttgcggatt aatcaatatt tttattactg tcacaaatat tcgaaaaatg
     1501 attattcggg cgattcccga aagtcttcaa catgcgattg gtggcgggat cggtattttc
     1561 gttgcgtatg tcggaatcaa aaatgcagga ttcttatctt tttcagcaga ccagtcagct
     1621 atttcgagct ctgtcgttga aggcggaaaa gcgacaaatg ttacgataaa tggtggaatc
     1681 gtccctgcgt tagccaactt tgacaacgct cctatcttat tggctgtgat tggtttggta
     1741 ctgacagcca ttttagttgt aaaaaatgtc cgtggggcta ttcttatcgg tatcgtagcg
     1801 accacagtcc ttggtatttt tatgggtgtt gttgatttaa gttccattga ttggcaaacg
     1861 aattcgttag gtaattcatt taaagaatta ggaacaactt tcggtgcagc atttggttct
     1921 gaaggcatgc aatctttatt cagtgattct tccaagattc ctcaagtatt gatgacgatc
     1981 atcgcattca gtttatctga tactttcgat acgatcggaa catttatcgg tacaggacgc
     2041 cgcacaggta ttttctcaaa agaagacgag atcgcgttgg aagatggtcg tgggttcaaa
     2101 acaaaaatgg ataaagcact atttgccgat gcgatcgcta cttctgtggg ggctatcttt
     2161 ggtacatcga atacaacaac ttatgtcgaa tctgcagctg ggatcggggc cggtggacgt
     2221 actggtttga cttcagtagt agtggcagtg ctattcgcat taagtagttt attctctcca
     2281 ctgatcgcca ttgtaccaaa tcaagcaact gcgccagcat tgattttagt tggagtaatg
     2341 atgatggctt cttttgcaga catcaaatgg ctagatatgg aagaagcttt accggccttc
     2401 tttgcatcga tctttatggg attgtgctat agcatttcct atgggattgc agcaggattt
     2461 attttctata caattgtgaa agtaatcaaa ggaaaataca atcaagtttc tgttgctttg
     2521 tggatcgttg atattttatt cattatcaac ttcatcattc tagcaacaat ttaactaaag
     2581 agaagggatt ggaacagtat ttgttctgat ctctttttgt tttacataaa aagaacgtta
     2641 catataaaat gacctttgcg ggatttttct ctttttactg gcgagatcgt tgcttgttta
     2701 ttcgaaaata ggtatgataa aagagacgaa agagggatga tagatggaga ttaattggag
     2761 aaaaaattta gtgattgcgt ggattggctg ttttttcact ggtgcaagta ttagtttagt
     2821 tatgccattc ataccagttt atgttgagca actaggaaca ccaaaagatc aaatcgaatt
     2881 gttttcggga ctagcaatct ctgtgactgc ttttgcatct gccgtggttg caccaatctg
     2941 gggaaatcta gctgatcgaa aaggccgaaa actcatgatg atccgtgcag cagcagggat
     3001 gacattaaca atgggatctt tggcttttgt accaaacgcg tattggcttt tgatcatgcg
     3061 gttttggaat gggattttgt ctggatatat tccaaatgcc acagcgatga ttgcttcaca
     3121 ggcacctaga gaaaagagcg gatgggcgtt aggaacattg tctaccggag cgattgctgg
     3181 aaatctgatc ggaccttcaa tgggcggtgc actggctcag tggtttggta tggaaaatgt
     3241 attcttgatc acaggggctt tgttgatgat cacaacggta ttgaccattt tcttagtcaa
     3301 agaagatttc catccaatcg agaaaaaaga tctgataagc accaaagaaa ttttttcaaa
     3361 aatggatcat ctctctattt tgatcggtct cttcattacg accttgattt tacagatagg
     3421 aatcactacg atcagcccta ttttgacctt atatattcgt gaattgagcg gaagtacaga
     3481 aaatatcctt tttgtcagcg gcttgattgt ttcgatagct ggtgtttccg ctgtcttttc
     3541 ttcaccgaaa ctaggaaaac taggggataa gattgggaat caaaaagttc taattggagg
     3601 actcatttta tcattttgct gttatttacc tatggcattc gtcactacac cgctccaatt
     3661 aggtatactt cgctttattt taggcttttc aactggcgct ttgatgcctt ctgtcaatac
     3721 actgatcagt aagattacgc ctcaagaagg agtcagccga atctatagct acaatcagat
     3781 gtttagcaac tttggacaag ttttaggtcc catgctagga tcgacagtcg cgcatgctta
     3841 taattacagt gctgttttta ttgtgacaag tttgtttgtt ctgtcaaata ttcttctttc
     3901 tttattcaac tttcgtaaag ttcttcgcag cagattataa tggtggtgag gagtagaaaa
     3961 taaaaaagag cttgagaaat ctcaagctct tggctgtttt tttacgaatg ggcagaggtt
     4021 tcttttcttg gcgcaactgc accaatcaaa aggataatga cacccgcaat gaatgaaatg
     4081 attgttgtca aagtaaaatc atatgttccg cttgttaatg cagcaccaat ataaccgact
     4141 acttgaccta gaacgacagc ccagaataat gtaacgatat atcgcataaa gaacacctct
     4201 tttctatctt aattttacca gattttcttt gaatgtaaag aaacgagacc aaatctcaat
     4261 aaaatttgag ttcttgcgaa agaactcatt cctacagatt acaagcaagc aaatgaagtg
     4321 gaataatcaa cttcagcgcc tgctatctta atgaaagtaa aaggatcaaa ccttttaacg
     4381 ctagtgaatt ttaacgaaaa aaaaggcgga atgcaagatg tttttcccac aactttatac
     4441 aattacttct tattctcttt tacaaagtac gatacgtatt cgagaatatg tacaagaagc
     4501 aaaaaaacaa ggctacactt cattagccat aacggataaa gatgtcttat acggtgcacc
     4561 tgaattctat cgtatttgtg tagaagaagg agtacagcca atcattggat tacatcttag
     4621 ttatacttat gaagaaaaaa attatggctt gcttgcgtac gcaaaagatg aaataggtta
     4681 tcagcagctg atgaaacttt ccagccgaaa aatgatccaa gaaaaattgg aacttgcaga
     4741 aatgacagaa accgaccatc tagcgtttgt tgttccggca aatcattctg tttctgaggc
     4801 tttcaataaa gaggataact ttttcggtca ttggcagttt ttacttgaaa atgtttttac
     4861 tagaaatttt tatatcggct tgacagaaaa cacgcctgat caattgcctg aatggtatca
     4921 gtatgtgata aaagaaaatt attcgctttg tgcattacat cccatcgcta gtttacaaca
     4981 agaagaactt tttgcacttg aagtgatgaa acatatcaaa gagggaagcc aaataccttt
     5041 agaaaattta tctgcatctt taccactaac agcaaatgtc tatcttcttt cagaacaaga
     5101 aatgatcaaa cgattcgaaa aagcgggtct ttcccaagct gtaataggcg cccagcgttt
     5161 agctgaatcg atttcatttg aattccagtt ccatcagaaa ttacttccgc attatccagt
     5221 tcctaatgga aaacaagctg gagaatattt aagagagtta tgctattcgc aagtggaacg
     5281 ccgaatcact gactatacaa aggaatacca agaacgtttg gactacgagt tatctgtcat
     5341 ccatcaaatg ggattcgatg attatttctt gatcgtatgg gatgtaatgg cttttgctca
     5401 tagaaacaaa atcgttaccg gtgcaggtcg tggttctgct gcgggttcat tagtagccta
     5461 tgtgttagaa atcacagatg tggatccatt agaatatgat ttgctgttcg aacgcttttt
     5521 aaaccccgaa cgctttacga tgccagatat cgatttggat attcctgaca atcgacgtga
     5581 agaagtattg aattatgttc gagaaaagta tggacaatat catatggcac aaatcgcaac
     5641 atttggcaca atggcagcaa aaatggttct tagagatgca gctagagtgt ttggtttatc
     5701 gcaaagtgaa gcaaaccgct ggtcaaaagc aatacccaat caattgaaaa tcacattgac
     5761 ccaagcttat aaaacttcgc atcaattagt agaattagtt caggcaagcg aaaaaaacca
     5821 acttttattc aaaactgcag tgattttaga aggattgcca agacatgttt cgactcacgc
     5881 agcaggtgta gttatcagcg ataagaatct acttgaacta gttccgctcc aacgcggttc
     5941 tgatgaagct tttctgaccc agtatacgat gaatgaggta gaagcaatcg gtcttttgaa
     6001 aatggacttt ttaggactac gcaacttatc gattattgat tttactttga aagctgttaa
     6061 gcgagtagaa gggtatgaga tccgattaaa agagatcccg ctaaatgatg caaaaacgct
     6121 gctcttgttt caacgaggcg aaacctcagg agtatttcaa tttgaatctg ctggtatccg
     6181 gaacgtttta cgaagattag gtccagaaaa catcgaagat attgccgcag tcaatgcatt
     6241 gtatcgacca ggccctatgc aaaatataga taccttcatt gccaggaaga aaggaaaaga
     6301 agccattcgc tacccagatg attcactgat cccgattttg aaaaacacat atggagtgat
     6361 cgtctatcag gaacaaatca tgcagatcgc ctcgactatg gctggattta cattagggca
     6421 gtcagatatt ctgcgacggg cagtgagtaa aaagaaaaaa gacgtattag atgaagaaaa
     6481 gtcgcatttt gtatcaggag cattacaaca gggatattcg gaaaaaacag cagcgaccgt
     6541 atatgaatat atcgaaaaat ttgcaaacta tggattcaat cggtcacatg catttgctta
     6601 ttcattcgtg ggattccaaa tggcgtatct aaaagcccat catccaggtg ctttctttac
     6661 cagcctgatg aattctgttc ggcataacac atcaaaattg aaagaatata ttgcagaagc
     6721 acgtaaaaat aaactcaaac tttcctcgcc gtccattaat caaagtaatt ccagttttga
     6781 gttggtcaat aaggaagaaa tcagattcgg actgacagcg ataaaaggta ttcggcgtga
     6841 ttttgtagaa gatatattaa acgaaagaaa acaaaatggc ccgttccagt cagtggatca
     6901 attcctcata cggatcgata agcgttggct gaaattagag ttactgcaat cactgacagc
     6961 agtaggcgtt tttgatgaat tatctcctaa caggaaacaa ttgatgcttg atctcgaagg
     7021 aaaaatccaa aatatcgttt acagtggtgg aagtcttgac ttgctgggaa tcatggcatt
     7081 aaaagaagaa gaagtcgctg actatacact agaagaacgc ttgaagctgg aagaggagta
     7141 cttaggtatt tatttgtcag gtcatcctgc agaagggttt gatcgtcttc gtttagctaa
     7201 acaagttcag ctgattacag atatcgtccc taatcaacca gtcaatttat tagtgatgat
     7261 caaggaagtt agagaaattc ggacaaaaaa aggagaactg atggcctttg ttgaaggaac
     7321 cgacagtagt ggagaaattt ctgtcacagt ttttccaact ctttatcgta aatatcgtaa
     7381 tttgtttgcc gttgatcgag tgatctttgt tcaagggcgg agcgaaatca gcaaatataa
     7441 tcaggaactg caactaattg gagaaagctt tgctgatcct aaccagttag aagcccagta
     7501 tccttcacaa acttgctatt tgcgtattac agaagaacgg gaagatcccg agataatgaa
     7561 acaattacaa gtgatttttc agaaacaccc aggatatata ccagtagtta tgtatcacga
     7621 aaaaacgcaa cgaaaagtag ttctgtcaga tgcttattgg gtggatgaca gcaagagtat
     7681 aaaggggcaa cttatttatc tgcttggcga agataatgtg gttttcaaat aacttacggt
     7741 aaaaaatact acttgctttc attttttttc atttttttat ccgtaaatca agacaagtga
     7801 gaaaaataat gttagaatgt tagtcgtggt taacgcaaaa atggttgtaa acctaagtca
     7861 tacttaggat aaagtatttt aatgaggtga atgtttatta tgaaacgcat cggaattttg
     7921 accagtggcg gagacgcacc tggaatgaat gcagctgttc gtgccgttgt tcgtaaaggt
     7981 atttacgaag gactggaagt atacgggatc aattatggtt ttgccggttt agttgctgga
     8041 gatattcgtc gtcttgatgt ggcagatgta ggtgacaaga tccaacgtgg tggtacattc
     8101 ttgtattctg cacgttatcc agaatttgca actgaagaag gtcaattaaa aggaatcgaa
     8161 caattgaaaa aattcggaat cgaaggtcta gtggttatcg gaggagacgg ctcttatcat
     8221 ggcgcaatgg ctttgacaaa acgcggtttc ccagcagtag gtattcctgg aacaatcgat
     8281 aatgatattc ctgggactga tttcacaatc ggttttgata cagcaatcaa tacagtacta
     8341 gaatcaattg accgtattcg tgacacagcg acttctcatg ttcgtacatt cgttatcgaa
     8401 gtaatgggac gcaatgcagg agacatcgct ttatggtcag gtgtggctgg cggagcggat
     8461 gaaatcatca tcccagaaca tgattttgat atggcttcag ttgctaaaaa aatccaagaa
     8521 ggccgcgatc gcgggaagaa acattgcttg atcatcttgg ctgaaggtgt aatgggcgga
     8581 aatgaattcg ctgagaaact ttcagaattt ggcgactatc atacacgtgt atctatcttg
     8641 ggacacgttg tccgtggtgg agcaccaagc gcacgcgacc gcgtgttggc aagtaaattc
     8701 ggtgcttacg ctgtcgaatt gcttcaagaa ggaaaaggtg gattatgtgt cggtatccat
     8761 gacaacgaaa tcgttgcgtc tgacatcatt gatacacttg aaaacaacaa acataaacca
     8821 gacttatcac tatatgatct aaataattca ctttcatttt aagagtgaaa gaaaccaagc
     8881 tatttaagct gtgtacagct ttaaataaac aaaattgaat gggagagttt tttaaatgaa
     8941 aaaaaccaaa atcgttagta cattaggacc agcaagtaac tcagtagaaa ttatttctca
     9001 attgattgaa tctggggcaa atgtcttccg ctttaacttt tctcatggtg accatgaaga
     9061 acaattatca cgtatgacta tggtccgtga agcagttaaa aagacaggaa aagatgtcgg
     9121 tattctttta gatacaaaag gagctgaaat ccgtacaact gttcaaggca caacagaagc
     9181 tgattttggt cgtgcaggat acatcaaatt tgaagtagga gatcaaacac gcatctctat
     9241 ggatcctgaa catgtaggtt caaaagaaaa aatcgcggtt acttacccag gattatttga
     9301 cgatgttcac gttggcggac acattttatt tgatgatggt ttgatcgata tgcaaatcat
     9361 tgaaaaagac gaagcaaacc gtgaattagt cgtagaagtt aaaaatgcag gtatgctagg
     9421 atcacgtaaa ggggtaaatg caccaggcgt ttctattagt ttgccaggta ttactccaaa
     9481 agatgctgac gatattcgtt ttggtttaga caacgatatc gactttatcg ctgcaagttt
     9541 tgtacgtaaa gcacaagacg ttctagatat ccgcgaaatc ttagaagaaa aagacatgac
     9601 tcatgttcaa atcttcccta aaatcgaatc tcaagaaggt atcgacaaca ttgacgaaat
     9661 catcaaagtt tctgacggtg ttatgattgc ccgcggagat atgggtgttg aaatcccagc
     9721 tgaactagta ccaatggttc aaaaacgcat catcaaaaaa tgtaatgctg ctggtatccc
     9781 agttatcact gctacacaaa tgttagaatc aatgcaagaa aacccacgtc caacacgtgc
     9841 ggaagcttct gacgttgcta acgctgtatt tgatggcaca gatgcaacaa tgctatctgg
     9901 tgaatctgca aacggggact acccagttga agctgtttca acaatggcac gtatcgacca
     9961 agaagctgaa aatgcattat cagaattagg tacattccaa ttgaacgaat tcgataaaac
    10021 agatgtaact gaaacaatcg gtttatctgt agctcgtgct gcaaaaaatc taggcgtgaa
    10081 aacaatcgtt gctgcaactg aatcaggata cactgcaaaa atgatttcaa aatatcgtcc
    10141 agatgcagat atcttggctg ttacttttga cgaacgcaca aaacgtggtt tgatgttaaa
    10201 ctggggcgtt tacccaacag ttgctgaaaa accatcaaca actgatgaaa tgtttgaact
    10261 agcagctaaa aaagctgttg aattaggttt cgcatcagaa ggcgatttaa tcttgatcac
    10321 tgctggtgta ccagttggtg aacgcggaac aactaacgtg atgaaaattc aaatgattgg
    10381 ttctaaatta ttagaagctc aaggcgttgg acaaaaatct gtcgttgcaa atgcagttat
    10441 tgcttcatct gctgaagaag ctgtaaaaaa agcaaaagac ggaatggttc tagttgtacc
    10501 atcaactgac aaagaatata tgcctgcaat cgaaaaagca gctgctgtta tcgttgaaga
    10561 aggcggattg acttctcacg ctgcagttgt aggaattgca caagatattc ctgtaatcgt
    10621 tggtgctaaa ggcgcaacaa caacaattac agaaggtgaa ttgattaccg ttgattcacg
    10681 tcgcggcatc gtttaccgtg gcgaaacaac agctatctaa tttagcttaa taaaaaataa
    10741 aaacagctct tcttttacga agagctgttt ttttgtgctg taacgaattc atggataact
    10801 tggactactt gttcttgaag aggcgtgacg atctgctgtg ttcgttttgc caatgaaatc
    10861 aagaagcgag gctgaacttc atctgaaaga gggaggcaaa ctaaatgatc ttcaggatgg
    10921 acagcaatct ctgccaaaaa accgataccc acattttctc ggaccatact ctttaaaatc
    10981 gtcaagtcat tgctttgata aacaactttt ggatgagtat gtgtttgttt agaaagctta
    11041 gtgaatgctg cggggtggac ataatgttcg ttcaatagta caaactgttc atctgctata
    11101 tctttgaaag agacagtatc ctgcttggaa agaggatgtg taggtggcac gacgatcatg
    11161 aaacgtttgt ccaaaagtaa ctctgtttct aaatattgat ccacgattgg ctgtgttgag
    11221 cctaatacag ctaaatcgat cctcccttgt cggatcatcc cgtataagtc ccgagagcct
    11281 ccatcggtaa tctcaatttg attcatcaat tcatgcttga taaagtaact tgacagttta
    11341 ggaaagtatt cattgccgat aataggaggc atgccaaaac gaagcttttt cttttgcaac
    11401 tgagtgattt cagctcttgc agtagaaatc tcttggagaa tggtttgtgc atggagaaac
    11461 agctgctttc cagaaggcgt gacgattaat tcgtgatgcg atcgatctct tgtaataagt
    11521 acggtgtcaa actctttttc aagacgttgt aatgcatatg taatagtagg ttggctcaca
    11581 cggaaataat tagccacttt cgtaaaattt ttttgtttga cgatttggac atagtattcg
    11641 atatctttcg tgttcatgaa gatcatccgc tcctttaatg gaataatgat tattgtaaat
    11701 gattctgagg atgatagcaa acagaggctt tctaataaag atttttctgt ttttttatga
    11761 actgcaaggt gtcaagaaaa tttcattgac aacatgcttt ttgccctgta taattgcttt
    11821 tgttgcttag gagtgatgaa aaatgaaatg gtctttattg gaattgagaa aataccagga
    11881 gacaccgtta acttttcatg agacgctcga cttgaaagcc gctctcatga agcgagacaa
    11941 tttaatactt gatgttgcgc cagtggaagt cgaaggtttg gtctcagttg ataagaaagg
    12001 ctatatcgtt cattacactg tgcagacgac tttgacagtt ccttcaacac gttcactaga
    12061 acctgttgaa ctgcccatgg atttttctgt agacgaagta ttcatgacag aagaacaatt
    12121 ccaaagtaga gatgaacaaa tgtctgcgga tgagattttg ctaattgaag gtccattatt
    12181 gaatttagca gagtcgattg aagacaacat cttattggcg attccaatgc gggtattgac
    12241 aaaagaagag gaagcagcaa cagaacttcc aaaaggcaat gattgggaag ttgtttccga
    12301 agaagaatac cagcgcagaa aaatggaaga agccgagaat aaaattgatc ctcgtcttgc
    12361 aaaactatct gaactttttg attctaaatc aaaagacgac gataacaagt aagattggaa
    12421 tatattgtgt ctaatttaga tacaagactt tacaaggagg tgtagcaaaa tggcagtacc
    12481 agctagaaga acatcaaaag ctaaaaaagc aaaacgtcgt acacactaca aattgacaat
    12541 caaagggtta aacgaatgtc caaactgtgg tgaaatgaaa aaaagccatc acgtatgtgc
    12601 aaattgtggt tactatgatg gtaaggacgt aatgtcaaaa gaagcttaat acattttaat
    12661 tagaaaatgt aaaacctcaa ggctaaccat ggagccttga ggtttatttt tttgtagtcg
    12721 gtaaataaaa aatatgttgt tctttagttt gcaatgtagc cgtgttaagc tataatgtaa
    12781 gagattaggg agaaatcccg tatgtaggag gagttcatat gtcaaaacaa caaatcggcg
    12841 ttgtcggtat ggccgttatg ggtaaaaatc tagccttgaa catagaaagc cgcggctatt
    12901 cagtggcttt attcaatcgt acaggggcta aaacaaccgc tgtagtagaa gaacacccag
    12961 ataaaaattt caaagcaaca tatacaatcg aagaatttgt agaatctatc gagaaaccac
    13021 gtcggatctt gctaatggtc aaagcaggac cggcaacaga tgcaacgatc caagagttgc
    13081 ttcctcacct tgataaaggc gatattttga ttgacggagg aaatacattc tttaaagata
    13141 cgatgcgtcg taatgaagag ttagccaact ctggcatcaa ctttatcggt acaggcgttt
    13201 ccggtggtga agaaggagct ttaaaaggac cttcaatcat gcctggtgga caaaaagaag
    13261 cctatgcgct agttgcacca atcttagagc aaatttctgc aaaagcagaa gacgaagcac
    13321 catgtgtcac atatattggc ccaaatggag ccggacatta tgtgaaaatg gtacacaacg
    13381 gtattgagta tggagatatg caactgattg ctgaatctta tgacttaatg aagaatattt
    13441 taaacctttc agtagaagaa atggctgata tctttaaaga atggaatcaa ggcgaattag
    13501 acagctattt gatagaaatc actgctgaca ttctgacacg taaagacgat gaaggaacag
    13561 gtaaaccagt agtagatgtg atccttgatg cagctgggaa taaaggaaca ggtaaatgga
    13621 caagccaaag cgcgcttgac ttaggtgtgc cacttccatt gatcactgaa tctgtatttg
    13681 cccgctatat ttctgcttat aaagaagaac gcgtacaagc aagcaagatt ttatctcgta
    13741 cgaacgattt tgagttcacg ggagacaaaa aagaattggt cgaaaaaatc cgagaagcgt
    13801 tgtatttcag taaaatcatg agttatgccc aaggatttgc tcaattacgt gtcgcttcaa
    13861 aagaatttga ttgggacttg ccatttggtg aaatcgcaaa aatctggcgc gctggatgta
    13921 tcatccgagc tcgattcttg caaaaaatta cggatgccta tgacaaaaat cctgagatcg
    13981 aaaatttatt gctagatgat tacttcgttg aaatcacgaa aaaatatcag caatcagtca
    14041 gagacgtcgt tgccttggct gtccaagctg gcgtacctgt accgactttc tcatcagcca
    14101 tcgcttactt cgattcatac agagcagaac gtctgcctgc aaatataatc caagcccaac
    14161 gcgattactt tggcgcacat acctatgaac gtgtcgacaa agaaggaatc ttccattact
    14221 catggtacca tgaagaataa tagtctttcg gctgttagga aatgagaaaa taaaaagagg
    14281 acgaaagagc tgaagtcgat tgttatgagt agacttcagc tcttttttaa aattgctatc
    14341 catgataaaa tgactataga tttttaagca tattatcggt ataatgatag agtttttaaa
    14401 aagccagtag aatagaaagg ataaaataat aaatgagcaa tattctaatc attgaagatg
    14461 aaaagaactt ggcacgattt gtcgagctag agttgaagca tgaagggtat aacgcagaag
    14521 tccactacaa cggccgaact ggtttagatg cggcattaaa taatgagtgg gatgccatat
    14581 tgttggatct gatgctccca gaattgaacg gtttagaagt ctgcagacgt gtacgccaag
    14641 tcaaaaatac accaatcatt atgatgactg caagagattc tgtgatcgat cgtgtatctg
    14701 gacttgacca cggagcagat gactacatcg tcaaaccatt tgctatcgaa gaattattag
    14761 cacgtcttcg tgcattgttg cgtcgaatcg atattgaagg cgataaaaat gtggctaaac
    14821 aaactactat tacttatcgc gatttgacta tcgaaaaaga aaaccgtgtc gtacgtcgcg
    14881 gaaatgaagt aattgaacta acaaaaagag aatacgagtt gctgcttact ttaatggaaa
    14941 acgtgaacgt ggtattagca cgtgacgttt tactaaataa agtgtgggga tacgaaacag
    15001 aagttgaaac aaacgtagtg gacgtatata ttcgttatct tcgcaacaaa attgacgttc
    15061 ccggggagga aagctatatc caaactgtac gcggaacagg ttatgtgatg cgctcgtgat
    15121 aaagccgaaa aaatcggaaa aaagagaact gaaagggccc tccttgacaa tcaagtgggc
    15181 ctttgcgagt tctttcttca tattcgttgt gtttacaatt ttcgctgtca ttacctataa
    15241 atcatctatc aatctgattg tggcaaaaga aagaaacaat gtagagcgta cgatttcaga
    15301 agtagcttct cgtttggcta actcggatga agaactgaca ttagtaaata cgtatcgtaa
    15361 tctgacgaaa gcgacaaatg acggagatga ggtcgtagat catacttcac tggtcgaagg
    15421 aaacttaatg aagatcgatt cctttatagc tgagctaggg cagccatcaa tggatcttta
    15481 tgtttatgat ttggacgaga acttgatttt taaaacccat gaaaacagtc gaaacctgat
    15541 tcagactaca cgtaaagttc caacgatcgt tacactagaa ggaaaaactg gctttttatc
    15601 tatccagcct atatattcta aaggtacaag agaaaagatc ggctatgcgc aaagcttcta
    15661 tgaattatct tctttctatg atattcgaaa taagctatta ttgactctta ttgttttaga
    15721 agttgtttct ttgatcttaa gtagtatttt aggtttcttt ttatcttctt atttcttaaa
    15781 accactcaag gtactcagag atacaatgga tactatacgt aaagatccgc aatcagatat
    15841 ccacatgcct gaaatcgaca caaatgatga attggcagat ttagctgaaa tattcaatga
    15901 gatgcttgac cgtatgcgtt tgtatattga acaacaagag caattcgtgg aagacgtatc
    15961 tcatgagtta aggacacctg ttgcaattat tgaagggcat cttagtctgc taaatcgttg
    16021 ggggaaagat gatcctgaga tattagaaga atcattaagc gccagcatgc aagaaattag
    16081 ccgtatgaaa agcttagttc aagagatgct agacttatca agagcagaac aagtagatgt
    16141 tcattatgcg aatgaaacaa ctaaaggaaa agaagtcacc tatcaagttt ataataattt
    16201 caagatatta tatccagact atgtgatcac tttagatgat gatttgacaa aagaagtgac
    16261 cctgcaaatt tatcgtaatc actttgaaca attaatcatt atcatattgg ataacgcagt
    16321 aaagtattca actacacgaa aagaagtcca tatctctatt tcttccactc tgaatgaatt
    16381 tgagatcgcg attcaggact ttggcgaagg aatacccaaa gaagacctta ataagatctt
    16441 caatcgtttt tatcgagtgg ataaagcaag agctagaacg aagggtggaa atggtctggg
    16501 gttgtctatc gctaaacaac tggttgaaaa ctataaagga cgtatcttag cagaaagtgt
    16561 agtagggcaa gggacaattt ttagaatctt tattcctatc gcaaaggcag aagacaagta
    16621 aagcatgtgc tttacttgtt tttttatata tccaacaaag aataaggtac aaaatacaag
    16681 caagttagaa attcagggat gtcattataa aaaaaactag cgaacaatta aatagaataa
    16741 c
//
LOCUS       DAZKCZ010000051        16728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000051 DAZKCZ010000000
VERSION     DAZKCZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.051"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..262)
                     /locus_tag="ACG8KN_001562"
     CDS             complement(<1..262)
                     /locus_tag="ACG8KN_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9188622.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTA"
     gene            complement(264..461)
                     /locus_tag="ACG8KN_001563"
     CDS             complement(264..461)
                     /locus_tag="ACG8KN_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP9188623.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(855..2582)
                     /locus_tag="ACG8KN_001564"
     CDS             complement(855..2582)
                     /locus_tag="ACG8KN_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFP9188624.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2575..3768)
                     /locus_tag="ACG8KN_001565"
     CDS             complement(2575..3768)
                     /locus_tag="ACG8KN_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9188625.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3955..4599)
                     /locus_tag="ACG8KN_001566"
     CDS             complement(3955..4599)
                     /locus_tag="ACG8KN_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9188626.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4693..4827)
                     /locus_tag="ACG8KN_001567"
     CDS             complement(4693..4827)
                     /locus_tag="ACG8KN_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFP9188627.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4829..6961)
                     /gene="feoB"
                     /locus_tag="ACG8KN_001568"
     CDS             complement(4829..6961)
                     /gene="feoB"
                     /locus_tag="ACG8KN_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFP9188628.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6958..7431)
                     /locus_tag="ACG8KN_001569"
     CDS             complement(6958..7431)
                     /locus_tag="ACG8KN_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFP9188629.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7832..8056
                     /gene="nrdH"
                     /locus_tag="ACG8KN_001570"
     CDS             7832..8056
                     /gene="nrdH"
                     /locus_tag="ACG8KN_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9188630.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8215..10374
                     /gene="nrdE"
                     /locus_tag="ACG8KN_001571"
     CDS             8215..10374
                     /gene="nrdE"
                     /locus_tag="ACG8KN_001571"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9188631.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10424..11389
                     /gene="nrdF"
                     /locus_tag="ACG8KN_001572"
     CDS             10424..11389
                     /gene="nrdF"
                     /locus_tag="ACG8KN_001572"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9188632.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11478..12617)
                     /locus_tag="ACG8KN_001573"
     CDS             complement(11478..12617)
                     /locus_tag="ACG8KN_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9188633.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12926..13639)
                     /locus_tag="ACG8KN_001574"
     CDS             complement(12926..13639)
                     /locus_tag="ACG8KN_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9188634.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13893..14594)
                     /gene="nagB"
                     /locus_tag="ACG8KN_001575"
     CDS             complement(13893..14594)
                     /gene="nagB"
                     /locus_tag="ACG8KN_001575"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9188635.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14828..16360
                     /locus_tag="ACG8KN_001576"
     CDS             14828..16360
                     /locus_tag="ACG8KN_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9188636.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16551..>16728)
                     /locus_tag="ACG8KN_001577"
     CDS             complement(16551..>16728)
                     /locus_tag="ACG8KN_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9188637.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5192 a         3442 c         2885 g         5209 t
ORIGIN      
        1 cagcagttct tccaatttca ctcaaatttc ttattaattc ctcttgatat tcagtgaatc
       61 ttattttatt agtataagca gtaaaatata gataattcac caatctattt attaaaggac
      121 tgggatctaa ttcatatttt tgtttttcta attttaaata taccttttgt aaaacctcaa
      181 gaatatcaga atatttagga tctttactta tagatatatc attatataat tcatgaacaa
      241 tttgcttagc attttttttc attttagcac ttccctggaa ttgctccacc taaaaaacca
      301 cctatagaca ttcctgcaat acaagtagtt gccttggccc aatcgaccgt acatttattt
      361 ttagtgcaat acactccatt tccataatat tttccactat gagtggtacc cccatagata
      421 agttgtgtct ctttaataga caaaattttt aaatgtttca taatctaatc acccctaaat
      481 taatgttttt agactattat tctaacattc attaaatttc aaaaaacaat tccttcttga
      541 aacgtagcaa aataattcct gaataagaat ttaatcactt ttaagtatat aaactatgta
      601 tattctttaa tattttttga gaatgcactt cgtacataat aaactttaat cacattagtc
      661 tttcatacct acgatcgatc tctttgactc gcacccataa attcataata gtttgtcggc
      721 gtctttgaac gtttcgaaga tgagctacgc attctctttt tttatagaca aaaaagatag
      781 agacgaacat ggagcttcca aatttggata tttgatccaa ttttggaagc tccatttatt
      841 tcgtgtctgt ctttttattt tttccagctt tcgactaatt cttggttctc tttgatccag
      901 tcttttgctg cttgctgtgg atcttttcca ttgttgattt ccaacataac tttttccata
      961 tctttttctg accaatggaa attgtctaat actttgtaag cttctggctg gtcttctttc
     1021 aatccttttc gaaccatcgt atggatctgc tcttcgcttc ccattccgtt ttcaggatct
     1081 tctaagtatt tcagatcata tttggcaaac atccaatgag gtgtccatcc tgtaactaca
     1141 attggttcgt gttttttgat cgcttgacct agtgcaacgg tcattgctcc tgaagaagat
     1201 gtctctactt tccagtcttt caagttgtca tatttttgga tcgtgttttc agcagccgta
     1261 actacaccag ctcctggctc gattcctatg attttcttac ctgcctgatc ggttaactca
     1321 tcgatcgaat ttacatccat gtaggctggc actgctaaac caacttttgc tcctgtcaaa
     1381 ttggcaccta ggtcttcgac ttgatcttta tattgtgcat attgtgaacc atgagtttta
     1441 ggtagccaag ctgaaaccat ggcatctgac tcgccatttg aaacagattt ccacatgatt
     1501 gaattatcta atggcgtcat cgttacatca tagcccattt gtttcaatac ttcaccgact
     1561 acatttgttg aagcaacttc tgtatcccat tcaacataag ataagttgat acgttttgtt
     1621 tctttggcag atgaaaaact ccctatacca atcgcaccta agatcactac tactacagca
     1681 ccaatgatca gtccttgacg ttttttactt ttctgtgttg ttgctcctgc agctttttta
     1741 ttgtttggct ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa gataactaat
     1801 gcaaccccgt tgacaaatcc attcccgact tgtgctcttt gcaatgctga taatacgccg
     1861 cgtcctaagc cgggtgcacc gatcatagag gcaataacga ccattgaaag tgacagcatt
     1921 gttgtttgat tgatccctgc catgatcgta cttttagcta aaggcaattc tagtttgaat
     1981 agtttttgac gacttgtgct cccgaatgaa tcagaagctt ctactaattc tttaggaact
     2041 tgtcgaatcc ctaaattcgt aaaacgaacg gttggtggca gagcaaagat gactgaggca
     2101 aatacccctg gaaccatccc gataccgaaa aatgcaactg ctggaatcaa ataaacgaat
     2161 cctggcattg tctgcataaa gtcaaggatc ggtttgataa tactttgggc tttactgctt
     2221 tttgccatca agatccctaa tggtacccca atgatgatcg agatcacact agataaaaga
     2281 acaagtgtga cagtattcat caaatcattc catagacctt ggttataaat aaaccataga
     2341 ccgattaatg tgaacaacgt aagtcctgga cgtttcttag aaataaagaa agctgctacc
     2401 gtcaataaca agataaacaa aggagctgga atcactaaca gtaaatttgt aattcccgac
     2461 atcactgcct gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac
     2521 cattctgtga tattttctac ccagtttgcc actggtaatt gatagttcaa tagattattc
     2581 atgttcactc acctcgcttg tttctgctaa tgcttcaatc acactacctc ggatgacgac
     2641 accttttaat tttccatcct ctacaactgc aagtggagtc ggtgagtcat aaataatatt
     2701 gaaaatatca cttaccacca tgtctttccc aatcaccgtc acattttcat ctaggaagtc
     2761 gatcaatgga cgtttttgct tcctagcttc taacgcttga tctgcagtga tgatcccttt
     2821 caggtgtctc tttctgtcaa cagccatcag catgctgact tcttcattac gcatccttgt
     2881 caatgcaacg tttggtccat cagattcgat attcgtcgtc aatgcaggta ccatgatgtt
     2941 ttgagccgta agcactttgg aacgatcgac ttcttccaca aattcacgta cataatcatt
     3001 tgctggattg gtcaatattt cttcacctgt tccgatctgc atgatttcgc cgtctttcat
     3061 caaggcgatt ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat
     3121 cgttttttga acattggctt gcaggtccag taattcgtct tgcatttctc gccggatcag
     3181 cggatcaaga gccgagaacg cttcgtccat cagcaagatc tcaggatcat ttgctaaagc
     3241 tctggctagg ccaacccgtt gttgcatccc accagataac tgactaggat attggtcttt
     3301 aaatgaaagc aaactagaat tttctaatgc tttttctgct ttttcttgtc gttcttcttt
     3361 aggtacacca cggacttcta acccgtattc tgtattttcc aagatcgttc gatgtggaaa
     3421 cagaccgaag ttttggaaaa ccatattgat tttatgacga cggacttctc gcaattcttc
     3481 tttgcttaac ttcgatacat cttctccatc aatatagata ctgcccgaag ttggctcaat
     3541 cagacgattc aatagacgga tcaatgtgga tttcccactc ccggaaagcc ccatgatcac
     3601 gaagatttct ccttctttta cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt
     3661 tttctttaaa atctccgttt tcggctggtg atcattcatc atatctaaag cggcttgtgt
     3721 ctttttacca aatatctttg ttaaatgttt tacttctact tttaccaaac aaattctcct
     3781 tttttatgta aattcatttt tctttctgaa aaaagtgacc acactatgtt aacatcatgt
     3841 agtcactttg tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa
     3901 tctttgtaaa taaaaggttt agccaagtta atattttttt aatatttcac acaatcattc
     3961 tgcaagctga tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc
     4021 aataaaatga acgatgtccc ctgccgtaaa tttagcatag gggccaggtg aaacaagcag
     4081 ttcttcatta tgagaaattg cgataactgt ggcgccagtc gcctgccaga tattcaactc
     4141 gctaattgaa cggtccagat gcattgcttt ttctgataaa gataattcaa acggaagtaa
     4201 aggattcatc ttatgtattc gcttcgtttg attgaccagc tgatccaata attgagaaaa
     4261 atgattcagc tcatcttgtt gcttctccac acttgccatg atttctgctt ttgtatcttg
     4321 gatggattgt acatcttgat attgcaatag gaaatctttt gctttttcct tcgaagcaac
     4381 aaatgcccca cttccatgcc gtacttccat gattccaaga tctaccagca cattgatagc
     4441 cttgcgggta gtctcaggag aagcattgta cgtactggca agtgttgagc gggcatggat
     4501 cttctcaccc actttatatc ggttttcaac gatacgttcg gctaaatcaa cagcaatctg
     4561 ctggtagcgc gggcggacga tttttttctt tccagacatt ttctttcctc tttctttttt
     4621 tactttccct catcttacta tgctctagca aaataagcaa aaaaggggag aatagcctct
     4681 tgcaacgact gatcatcgct tgatctgttc cttttttact gggcaagctg tttgacagtc
     4741 atcacaattc ttaccggatt ttaaacgagt atagacaatt gcgcctgcac ttccaaagat
     4801 taacacactt aataagaatg tcgccatcct attcctctcc ttctaatgac aatatagtat
     4861 aaaactggtt cttggatttt ggctgacgga acaaaacata aacgagtcct atcatcagta
     4921 aaatagcaag atacgtccct aacgctacct gtccaccctc caataagtgt cccaactgat
     4981 aaatcacaaa actaactaaa taagctaaac cgcattgata gcctatggcg atccaagtcc
     5041 atttcttggc attcatttcc cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa
     5101 gtaaattgaa ggctaacaat gaataagctg ctgcgggagt atatgtgtgt tgtagcactt
     5161 gccagatttc tctgccattt tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc
     5221 caataatatt ttctttagcg accaatcctg taatcgctgc gactgttcct tgccagtttc
     5281 cccaaccaag cggctgaaaa acaactgcca ggattcgccc gaaaaaggct aagatacttt
     5341 ggtcttcaga aacaggttga gcataaaaat tataagaaga gctgaaccag atcacgatac
     5401 tagaaacaaa gataatcgtt cctgcttttt taacaaaaga cttgctgcgg tcataggttt
     5461 gtcttaggac cgctgaccat tgaggaaaat gatatgcggg caattccatg ataaaaggag
     5521 ctgtatctcc agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa ataattgcag
     5581 tcataccaag aaaataggca gaaggagcta cccaagattg atgaggaaaa aaggcccctg
     5641 ctactaaggc aataatcggc aactttgcag aacaaggcat gaaggttgtg accatgatcg
     5701 tcaaacgacg atcttgctcg ttttcaatcg tgcgactggc catgacgcct ggcactccac
     5761 atcctgtagc aatcagcata ggaatgaagg attttcctga aagaccgaat ttcctgaaaa
     5821 gtcgatccat gacaaaagcg atcctcgcca tatatccgca gtcttccaga aagctcaagc
     5881 ataaaaacaa tataatcaat tgcggtagaa atcctaacac tgctcccacg cctgcgatga
     5941 tgccgttcaa gatcagctcc tgcatccatc ccgctacttg ccattcagct aacaatctgc
     6001 caacatgttc tggtaaccat gtaccaaaca atgtgtcatt gatccaatct gtccccattg
     6061 ttcctactgt ttgaatagcc agataataga tcagccacat cacaaatgcg aagatcggca
     6121 aggctaacca acgattcgtt gcaatctgat cgattttgtc actcatcgat aattggaaag
     6181 aatcatttga cacaacacag agtgtacata gttgggtaat aaattcataa cgttcattga
     6241 ccagaatcgt ctcactatcg tcgtccaata gtttctctgt taactggatc agttgctcga
     6301 tctctttttg ctgagaaact gctatctcgt aatcctctct taccttctga tctcgttcaa
     6361 aatatttcat tgcataccag cgtttcttgc cgctttgttc tcctagtaca tcttcaattt
     6421 catgtaaagc tgcttctaaa cgtggatcat atgtacaggg aacaggaata gaaggccgct
     6481 tttttgcaga ctcctgaatc ttttcgatta gtttttcgat tccttgcttt ttcaatacgc
     6541 tgattggtaa aactggtaca ccaagaccat aggaaagctt ttctagattg attttttttc
     6601 cttctttttt gatgacatcc atcatattca gaccaatgat cataggaatg tctaaatcca
     6661 ataactgcgt agttaaataa agatttcgtt ctaaattggt agcatcaata atgttgatga
     6721 tcagttcagg ctgttcctga accaaataat ctcttgcaat cacttcttct ggtgtataag
     6781 gagacaaaga atagatgccg ggtaggtcct gaatatgaat gttcggattt tttttcaacg
     6841 atccttcttt tttttctact gttacgcccg gccaattccc tacatattga ttcgaaccag
     6901 tcaagagatt gaacaagcta gttttcccgc tgtttggatt tccgactaac gcgatattca
     6961 ttctttttcc tccctttgta tcaagattag ttcggcctct gactttcgta acgtcagttc
     7021 ataccctcgc actgtcagtt cgattggatc tcccagaggg gctagttttc ggataactat
     7081 ttttacattt cgcgtcaatc ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg
     7141 gattgctacg acgatcccag tctctcctgc ttttaattga tcaagagaag tccagctttc
     7201 agaggaagca gtctttttat tgacaaagat ttgacctagt atttcccgac ttactgccac
     7261 tcgatgactt tttaccagta aaatggcatt gttgcctgat aaattgacga cgacgatttt
     7321 acttccggca acgatcccga tattttgcaa atggcgtttt gttttttctt ctgtttcgat
     7381 tcgttcgact tgataaacga aattctgctt tgcctgcgct aaggtctgca tattgattcc
     7441 tctcttgtga aataaatcat gtattaaaat cattcattat atgttctaat tttcttacgt
     7501 gttacgcaaa taaatgagaa tcattctcac atagaatact ctcggtaatt gagaatgtca
     7561 attatacttg tgatattatg agcgaatctt tagtattgat agataaaaat gagaaaaaaa
     7621 tatttcttca tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat
     7681 acttgttttc agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca
     7741 caatatgtag tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga
     7801 taataagaat gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa
     7861 ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga
     7921 agtcaatatc gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag
     7981 tgttcctgtc attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg
     8041 taaacttgcc agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct
     8101 tgagacagca ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat
     8161 gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt
     8221 cttaaaaatt tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc
     8281 aatggtcaaa tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat
     8341 gtcaaaccaa atacgatgac ttttccatct attatggaaa aaatcaatta cttgatcgat
     8401 caagaatata tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta
     8461 tatcaatttt tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt
     8521 tattctcaat atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat
     8581 cgagtagcct tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt
     8641 gcagacgaaa tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga
     8701 cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac
     8761 tctatcgggc gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg
     8821 attactttat caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca
     8881 agtggtgtcg ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta
     8941 ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct
     9001 ttcctttcag caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt
     9061 ggcgtgatcg tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta
     9121 tttagtccat atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca
     9181 gctgagtatg atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt
     9241 gatcttgaaa atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat
     9301 attgatacag cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt
     9361 tcagagattt tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta
     9421 ttaggaacag atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc
     9481 cctaactttg gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca
     9541 tctgatatcg atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga
     9601 ttaggtgcca tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag
     9661 gattcattag actttacaaa tatttacttc atgctgttga attattggac acttgttgaa
     9721 agcaacaata tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat
     9781 gctgacggca cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa
     9841 gtcaaagcat tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa
     9901 caagcagtaa tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc
     9961 tcaatttcat atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa
    10021 gaacgccaag agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag
    10081 acgattccgt attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat
    10141 gcaacggcac aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa
    10201 atcccagaag gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat
    10261 attttaagac attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt
    10321 actgatgatg cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat
    10381 agatagattc gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta
    10441 tgaagcaata aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct
    10501 gacagagcaa ttttggctag atacacgtat cccactttca aatgacctag atgattggcg
    10561 tacattgtct caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct
    10621 agatactgtc caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca
    10681 tgaagaagcc gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc
    10741 ttctattttt agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa
    10801 tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac
    10861 accgctagaa aagaaaattg ccagcgtctt tttagagaca ttcttgttct attcaggttt
    10921 ttatactcct ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa
    10981 actaatcatc cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg
    11041 attcaacgag ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct
    11101 ttatgagctt tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg
    11161 gacagaagaa gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg
    11221 gatggatcca ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat
    11281 ttcaacaggc acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg
    11341 tcatgtagaa gcgatgaaag acgacgatta tttgattgga ttggaataag gatttatttt
    11401 aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta cagatatttt aaatgtacac
    11461 tgaactcttt tttatgctta ttcacttgtt tcttccagca cttttggact gtctaacttt
    11521 tccgaaacat gttccattct tctcaataaa tagattttcc ggatatttaa tattatcgtc
    11581 ttaatcactg catagacagg tatgcaaatc agcatgccta atattcctgc gacacttcct
    11641 gctccaatca ataccaagat gatcgtcaaa ggatggatat tcattgattt cccaaataac
    11701 aacggcttga tcaaatttcc atctaattgt tggatcacta aaatcgacaa cgccatatac
    11761 aaagcttgaa atggagagat aaacaaaccg acaatgattg ctggtaccgc tccaataaaa
    11821 gggccgacat acgggataat attcgtgatt ccgcaaatga ccgcaagcaa caagctataa
    11881 ggctgatcga atattctcat tgccaaaaag ctcattacac caataatcaa agcatctaag
    11941 atcgtactgc tgatataggc agataatgtt tcgtttaatt cttttaccgt ttggcgtaac
    12001 tctttacgaa tcgctttcgg aaagaagtga gacaacgcat caataaattt gtgcccatcc
    12061 ttgaacataa atagcaaaat aaatggaacg gtaaataaca aaataaaaaa ttgcatcata
    12121 aatgaaatga tttttgagac acttgcagat aaactgacta atatgatatt gaacaaatta
    12181 ctaagagata aatttgctga tgctaattgt tcttccaaat tgaaattttt aaactcttcc
    12241 cgctgcgcca attcatttaa ccatttgctt gtttcatctg catacattgg caagctctgg
    12301 gtcaattgaa ttgtctgttc cattaactga ggcactacat tcatgacagt taaaacaact
    12361 aaaatgatta cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt
    12421 tcgagaaaaa caacgattgg atcgaacata taatagataa aaccagcaac aagcaatggt
    12481 aaaaaaattg ccgaaagcat tttcaatatt ggactaaaaa tattcggcat ttgtaaaata
    12541 aagtacacgc cgatgatcgt gagtactagc tccactgtcc agaaaaataa ttttgattgt
    12601 ttaaaacgtg attgcataca tctcctccaa ggtcctttga tacttctatt ctatcattta
    12661 acaaagagtt acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa ataaacatat
    12721 tttaatgagt agacgagtac ttaaacaaaa acacggtttg gagtagtgac aaaaattata
    12781 ctgcagtccc tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt aataagccca
    12841 gccaaacgaa aaatataagg aactattttt atttggctgg gcttattact tgatgtagga
    12901 catctatttt acaacctcag atcatttatt gcgcaatact gactaaatgc aactttgtga
    12961 tatttttgtg tacggacaac tcctcgatca catcggcata agtgagccct tttggaagat
    13021 caatcgtata gatatttttg tacactcggt agtcatcaac gaattttacg tcaaaattca
    13081 catcttcgat tacgatattt ttctcttcaa aataatcggt aagaaaatct ttggtttctt
    13141 ttctatggat aaattggatt tctaactttt tagtcgtagg tacatggatc acgcgtttca
    13201 ctactgtgag tgcaatagca atcccgataa aactgatgat cgcaatggaa tagtaaccca
    13261 tgccgatggc gatccctaat ccagcaattg cccataaaga agcggcagtc gtaagtcccg
    13321 tcaccgattg tttcgtgacg atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca
    13381 cttgagcaat cagccttgct tcatctgaac gaatcgttcc agcaagctgt ggatactcta
    13441 aagcatctct taatgcacca ctagcgatct caacttggat caatgcaatg atacatgctc
    13501 ccatacaaac taaaatatgc gtacgcatac ctgctggacg atttttatat tgtcgttcaa
    13561 aaccgattgc tccgccaaat agcatagcga tccctaaacg taaaataatc tctggaattg
    13621 tcaataccat atcattcatc taaacaccct ccagtccaat atatcatgtc tgaaaggagc
    13681 agacaaaaaa agtgcaattt ttacctctta cgaaaaaagg actgtgacaa aatttttgtc
    13741 atagtccttt ttatgaataa acggtgttca atcagctgac cctcggtatt actcaacaaa
    13801 cggcaaaata tggggagcta tttttccttt gtcttctcta atactcaggt caaaacgctg
    13861 atttcacaac ctctttagcc tactattgaa aattacaact tacttgctgc ttcttcatcg
    13921 atgatcacaa caacattgtc gtgattttgc aatacacttg ctggcaaatg attcgttaca
    13981 ggtccttcaa tcatcccttt gattgcatca gctttgcttt cgccaaatgc catcaagacg
    14041 atttctttac ttttcataat tgaaccgatt cccattgaaa cagcttttgt tggtacatct
    14101 tctgctttat cgaagaatcg tttgtttgca ttgattgttg attctgttaa gtcaactact
    14161 gaagtcgttc cgtcaaatga agcaccaggt tcattgaaac cgatatgtcc attttgcccg
    14221 attcctaaga tttggatatc gattggatgt gcatcgatga tttgatcata ctttttgcat
    14281 gctgcttcca gatcttctgc ttttccatct ggtacaaacg tttctttgaa tggtttttta
    14341 tcaaataatt ggacatccat gaaatgacgg tagctttgtt catctgaacc gcctagtcca
    14401 acgtactcat ctaagtttac agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt
    14461 tctttatata aagtaactgg tgtgcttcct gttgccaatc ctaatacttt tgcacctttg
    14521 ttcatacctt ctttgatgat ttcaaatgct ttttttccgc cttcttcagc gttttttaca
    14581 cgaatgattt ccatagtgat tgctccttta tattttggta tagcccaatt atagcattct
    14641 ttttttcttt ttacaataat tagtatagac caaattgcat ttttttcatt ttcttgacaa
    14701 taaataggaa tccgtgttag gtttcataag atggttcaat caaacaatcc atgttgcatg
    14761 aaaaccaagc aagtgattca ctgtggacgc tttatgtctg cagttttttt atgaaaaagg
    14821 agttattatg agtatactga cattaaacaa cattacccag caatttggcg acaaaatcct
    14881 ctatgaaggt gtcgatctgc aattaaatgc tggcgaacat cttgggctga ttggacaaaa
    14941 cggagccggt aaaagtacat tgattaaaat catcactggt gagatcttac cagatgacgg
    15001 acagatccat tggcaaaaaa atattcacaa aggttacctc gatcaatatg tggaagtcga
    15061 tgaaacatta acaatcgaag aatttctaaa aactgcgtac gcaaaagaat acgaaaagga
    15121 agaaaaaatt gcttttcttt accaaacata tagcgagacc ttcgatgatt ccttgctgga
    15181 aaaagctggg aaactccaaa cggaattaga ccaaggagtg ttttatcaaa tcgacacctt
    15241 agttgctgag atgagcagcg ggttggggat cgatgtctta ggtttagatt cactgttgaa
    15301 aaatctaagt ggtggacaac gttcaaaggt gattttagca aaactacttt tagaaaaccc
    15361 agatgttctg atcttagatg aaccaaccaa tcacttagat gatcagcata tccaatggct
    15421 gacgcagttt cttcaggatt ttgacggtgc gtatatcgtg atttcccatg accaagaatt
    15481 tcttgatcag atcaccaccc atatcgcaga catcgagttt ggaaagatta ctaagtatac
    15541 tggccatcta aaacaagcat tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca
    15601 atatcatgct caacaaaaac aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc
    15661 cggttcccga tcaacgatgg caaaaagccg acaaaaacaa ttagataaaa tcgaacggct
    15721 gacgcctcca gcttcagcaa gtaaagctgt ctttgatttt ccattctcgc ctattgtcac
    15781 gacattagca gtggaaacaa ctgatttagt catcggatat gatcgaccgc tacttgcacc
    15841 catcaatttg accatccgct ttggtgaaaa tattgccatt cgaggcttca acgggattgg
    15901 aaaatctact ttattaaaaa cactgatcgg tgagattgaa aaaataagtg gcgatttcca
    15961 cttcccagat aacacaaaaa tcaattactt ttcacaagat ctgtactggg aaaatccatt
    16021 agaaacacca ctacaatatt taagcagtca atttccaaaa gcgactatca aagaattacg
    16081 tcgccaacta gctaaagcag ggttagttaa tcaattagca agtgaacctc tcactacatt
    16141 aagtggtggg gaacaaacta aggtgaaact tgcacagttg acaatgaacc aaggtaattt
    16201 actgattctc gatgaaccga cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga
    16261 cgggatacag aaatttccag gaacagtaat catcgtctct catgaacaag aattttatca
    16321 agatcttgtg caacgagtca ttgagattga accaaaataa acgtttgatt gaatagttga
    16381 aaaaaaggag ctgtgacaaa agttttgtca caactccttc ttctgaataa atggtggcca
    16441 attagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggagct atttttattt
    16501 ggctattctt attacttgat gcagaacagc ttttcacaac ctcttttcgt ctatttttta
    16561 ttttcgagat ttctctttcc tttaaaaaca actgtttgaa agaagaacgc acatccttga
    16621 aaaacaagcc cgctaaccgt tacacctaac cagccatact gctgccacat tgtcgtccct
    16681 agcaatgaac cagccgcacc tccaataaag tacataaaca taaataca
//
LOCUS       DAZKCZ010000052        16583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000052 DAZKCZ010000000
VERSION     DAZKCZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.052"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(386..1321)
                     /gene="murB"
                     /locus_tag="ACG8KN_001578"
     CDS             complement(386..1321)
                     /gene="murB"
                     /locus_tag="ACG8KN_001578"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9188638.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1394..2314)
                     /locus_tag="ACG8KN_001579"
     CDS             complement(1394..2314)
                     /locus_tag="ACG8KN_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9188639.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2427..3179
                     /locus_tag="ACG8KN_001580"
     CDS             2427..3179
                     /locus_tag="ACG8KN_001580"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9188640.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            4043..4576
                     /locus_tag="ACG8KN_001581"
     CDS             4043..4576
                     /locus_tag="ACG8KN_001581"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9188641.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(5066..5590)
                     /locus_tag="ACG8KN_001582"
     CDS             complement(5066..5590)
                     /locus_tag="ACG8KN_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFP9188642.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5583..6056)
                     /gene="tsaE"
                     /locus_tag="ACG8KN_001583"
     CDS             complement(5583..6056)
                     /gene="tsaE"
                     /locus_tag="ACG8KN_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9188643.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(6179..7159)
                     /gene="pta"
                     /locus_tag="ACG8KN_001584"
     CDS             complement(6179..7159)
                     /gene="pta"
                     /locus_tag="ACG8KN_001584"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9188644.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7312..7992)
                     /locus_tag="ACG8KN_001585"
     CDS             complement(7312..7992)
                     /locus_tag="ACG8KN_001585"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9188645.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            8118..8987
                     /locus_tag="ACG8KN_001586"
     CDS             8118..8987
                     /locus_tag="ACG8KN_001586"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9188646.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            9013..9528
                     /locus_tag="ACG8KN_001587"
     CDS             9013..9528
                     /locus_tag="ACG8KN_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFP9188647.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9575..10138)
                     /locus_tag="ACG8KN_001588"
     CDS             complement(9575..10138)
                     /locus_tag="ACG8KN_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9188648.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(10193..10372)
                     /locus_tag="ACG8KN_001589"
     CDS             complement(10193..10372)
                     /locus_tag="ACG8KN_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188649.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10449..10958)
                     /locus_tag="ACG8KN_001590"
     CDS             complement(10449..10958)
                     /locus_tag="ACG8KN_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188650.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(11054..11434)
                     /locus_tag="ACG8KN_001591"
     CDS             complement(11054..11434)
                     /locus_tag="ACG8KN_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9188651.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11764..12513
                     /gene="pnuC"
                     /locus_tag="ACG8KN_001592"
     CDS             11764..12513
                     /gene="pnuC"
                     /locus_tag="ACG8KN_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9188652.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12697..13974)
                     /locus_tag="ACG8KN_001593"
     CDS             complement(12697..13974)
                     /locus_tag="ACG8KN_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188653.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(14053..14697)
                     /gene="pcp"
                     /locus_tag="ACG8KN_001594"
     CDS             complement(14053..14697)
                     /gene="pcp"
                     /locus_tag="ACG8KN_001594"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFP9188654.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14896..15840)
                     /locus_tag="ACG8KN_001595"
     CDS             complement(14896..15840)
                     /locus_tag="ACG8KN_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFP9188655.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15842..16528)
                     /locus_tag="ACG8KN_001596"
     CDS             complement(15842..16528)
                     /locus_tag="ACG8KN_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFP9188656.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4782 a         3429 c         2910 g         5462 t
ORIGIN      
        1 ctacataatt ctttttctca aattctaaat atctagctag cagcagtttt tctctatctt
       61 cctcttctat aaggtaatcc aacttcatat cctacacctc aaaaaatgcc tcataccaat
      121 tacttgatat gcagatgaaa gttcatcttt tcacctatac tgattttatg atggattatg
      181 cttgtttttt catcagttat tctcattttt tatctttttt ttgagaatat tgcaaaaaat
      241 ggaaaaattt atgaggaaat attctatatt ttttgtttta ttgtgttaaa atgtacaaga
      301 tttgcgccat ttgcgtaaac aaccacaaaa aagagcagga cggccaccgt cctgctcttt
      361 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
      421 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      481 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      541 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      601 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      661 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      721 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      781 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      841 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      901 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      961 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
     1021 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
     1081 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
     1141 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
     1201 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
     1261 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
     1321 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1381 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1441 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1501 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1561 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1621 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1681 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1741 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1801 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1861 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1921 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1981 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     2041 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     2101 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     2161 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     2221 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     2281 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     2341 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2401 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2461 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2521 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2581 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2641 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2701 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2761 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2821 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2881 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2941 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     3001 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     3061 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     3121 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     3181 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     3241 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     3301 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3361 ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta
     3421 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3481 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     3541 ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa
     3601 tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac
     3661 cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca
     3721 aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca
     3781 ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg
     3841 aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca
     3901 tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat
     3961 aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat
     4021 ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca
     4081 tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga
     4141 tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca
     4201 cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat
     4261 ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa
     4321 ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg
     4381 tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt
     4441 gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc
     4501 atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact
     4561 tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt
     4621 tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt
     4681 gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct
     4741 gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg
     4801 ttggacttat taccgcagat gcaagctttt ggacaccgtt taccaagagg ttgtgaaatc
     4861 agcgttttga cctgagtatt agagaaacaa acggcaaaat agttcctcat attttgccgt
     4921 tttttgagct aataccgaag gtcagctgat tgaacaccgt ttattcaaaa aaaatggact
     4981 gcgacacgac ttttgtcaca gtcccttcgt gtgtttttct gccttctcaa aaggatgaca
     5041 gtttctcact cttttcctat ttcttctatt caatcaaata actcatttta tatacatcta
     5101 aaaattcacc tagatctgtt cttgcccctc taggcatgac cgcttcgatt tggaagccca
     5161 tttttcggta aaggtgtacg gctctttcat tacgaacttg aacgtcaagc tccaatcgaa
     5221 aaagtacacc catttccttc gcccaactga tgatttcttc tagcataaga gtgcctagtc
     5281 ccattcccca atattcctgc agaatactga taccgacttc tcccacgtga gacaaacgaa
     5341 attgactgtc tgccttgaca gatgctgttc caataatcgt cccttcatag attgcaagca
     5401 gcaataaatt attattgctt tcgtatatat aatccaactc ttcttctaag gtttctggtg
     5461 gtaacaggag ttctgcttca tccaaaacta aaaattctgt ctccttatta acttgtccca
     5521 tcatcaaaag aatgtctttt gcatctcctg gcactgcttc ccgcaagatg atcccaactt
     5581 cttcactcat gattttcagc ccatttttct agaattcgct gtttgaaggt ttcactttgc
     5641 tccccgtatg agattagttt cacataccgc ttttctaaat ctgtatcact tttttctaaa
     5701 atcagttcca aataatcctc cggcagtgct tcttctaata gatttcccca ttcaacaacg
     5761 gaaagcccat caccttcaaa atattcatct aaccctaaat ctgctccgct agctgctacc
     5821 cgataaatat ccatatgata caacggaatc cgaccttgac tatattctct aataatcgtg
     5881 tatgtcggac ttttgatcat ttgcgttatc cctaatcctt gggcaattcc tttcgtcaac
     5941 gttgttttac ccgcacctag atcaccagtc aatatcagat tgtcacctgg cattgcggaa
     6001 tgaccaattg cctctgcgaa ttgttctgtc attgttaatc ctgatagttc gatcattttt
     6061 catcttctcc cgctcttctt tctttttgcc agtatatcgc gtaatgaagt ttttttaaag
     6121 aaaaaatgaa ctagtcaaga acagcagctg ctgtttttgg ctagttcgcg tgtatttgtt
     6181 aatcaatcaa tgtttgtgct gctgtgataa tcgataattt atatacatct tcttcatttg
     6241 ctccacgaga caaatcagaa accggtttgt tcaagccttg tagaattggt ccgattgctt
     6301 caaagttacc gaaacgctga gcaattttat agccgatatt tcctgactgc aattctggaa
     6361 atacaaagac tgttgcttga ccagctactt ctgaatctgg cgctttcagt tgtgcaactg
     6421 aaggaacata agaagcatca aattgcaatt cgccgtcgat catgtattgt ggcgctagtt
     6481 ttttagcgat tttagttgct tcaactactt tgtctacttc tggcgatttc gctgagcctt
     6541 ttgtcgagaa gctcatcatt gctacttttg gttcgatatc gaacaattct gctgtttttg
     6601 cagaatctac ggcgatttca gccaattctt gcgcagttgg attcacgttg atcgcacaat
     6661 ccgaaaagac atatttttct tgatctcttc cacgtaccat gatcatcgct ccgcttgtcc
     6721 gactgatgcc aggctttgtt ttgatgatct gaagtgctgg gcgaacagtg tcgccagtcg
     6781 aatggatcgc tccagagacc attccgtctg caatccccat atacgtaagc atcgtaccaa
     6841 agtaattcac atcttttaaa atcttttctg cttgttcttt ggatgccttt ccatttctac
     6901 gttcaacaaa agtatccacc atttcatccc acttatcgta attatcaggg ctgatgatcg
     6961 taaagctaga tgttttgatt cctctggcat gcgctgcatt tcttatttct tcttcgttcc
     7021 ctactaaaat gggttccaca agttcttctg ctttcaaccg ggcagctgcg cctaaaatac
     7081 gagcatcagt agcttccggg aaaacgattt tgatcccacg gcgaacaatt ttaaatctta
     7141 aactatcaaa caattccaca agatgacctc cagacgtttt tatatgtatt atcatacact
     7201 attccagaaa aaaagaacag tttattttat ctgtatcagt caaaaatgct ggaaaaatta
     7261 agtttttcac atacaaagcg aactgcacta taacaggaaa gcgctgtttt attacactgt
     7321 ttcaggcagt tgccaatcaa tcggttcttc tcctaacgca aggagcgcat cattcgtttt
     7381 cgagaagggt ttggaaccaa aaaagccacg acttgcagat aacggactgg gatgtggaga
     7441 tgttatgatg atatggcgac tcttatcgat cattttgatt ttttcttgtg ccggttttcc
     7501 ccacaagata aacacgattg gtttttcccg ttcatttaat ttttcaatga tcgtgtctgt
     7561 taatctttcc caacctttcc cgcgatgaga atacgcttgc ccttcccgaa cggtaagaac
     7621 ggtgttgagt aaaaggactc cctgtttagc ccatgacact agatttccat gttgaacagg
     7681 tgcgattcca agatcatcat aaagctcttt atagatattt cttaacgatg gcgggatctt
     7741 tacgccaggt tgcacagaaa aagataagcc atgagcttga ttttctccat gataaggatc
     7801 ttgtcctaaa atcaccactt taacttcttc ataaggagtc aattccaatg cttcatagat
     7861 atgatacata tctggatgga tcttttggga cgcatattct ttttttaaaa attctcttag
     7921 atgcagataa taatcttttg aaaattctga ggacaaaact tcttgccaac tgttatgaat
     7981 gatttctttc aaaaactcca cctcctaata aaaagaataa ctcacatttt cttcaatcac
     8041 aagggacaat cttcaccctc agcacttgat taatatggta aactaggaac aagaagcaga
     8101 aaggaacgat aatcattatg atcaaattaa ttgcatcaga tatggacggc acattattag
     8161 atgcccatat gagtgtttct cctgaaaatg ccgaggcgat acgttttgca aatgatgccg
     8221 gtgttgagtt catggtcgct actggtcgaa actatcaaga agctcgcgca gctttagatg
     8281 aggtaggaat cgattgtgcg atgatcactt tgaacggggc acaggtattt gataaagaag
     8341 gaaattcatt gtttaccgtg tcgattccaa ataatcaagc aatctctgtt ttagatattc
     8401 tagatgctaa cggtatttac tatgaagtag caaccaacga tggtctttat tctgaaaatc
     8461 aagcgaaacg aattgaaagt tttgcttcca tggtggctac ccatttacct caccttacgt
     8521 ataaaatggc gattgccatg gcctcagcta agctggagtt attgcacatc acatacgtgg
     8581 acagtattcg tgctattttg gaagaaaaga atctggaagt tttaaaaatt atttgttttc
     8641 atacagaagg gccagctgta ttaggtccag ttggaaagca gatttctctt ttaggcgatc
     8701 tagccgttac ttcctctggt caaaacaata tcgaagtgaa ccataaaaac gctcaaaaag
     8761 gaattgctgt tgcccatgtc gctcacgagc gtgggatttc tttagaagaa gtcatgacga
     8821 ttggcgataa cttcaatgat gtcagcatgc tgcaagtagc aggagtcagt ttcgcaatgg
     8881 gaaatgcaga aatcgaagta aaagattatg ccaaatatac aacggacaca aaccttgaat
     8941 ctggcgtagg aaaagccata ttgcgcgcga ttgcagaaga cttgtagttt tatctagaaa
     9001 agagggatat agatgtcaga atttttcatt caggaaaagc agcttggacg tgcaactcgg
     9061 acaattgtca aaaacgaaaa aggacattcc ctgtttttga tggttggtcg ctggggaaca
     9121 aagggcgatg cattgtctct ttatgccatg aatggcgatc tcgttgctca catcaagcag
     9181 ataagcctga cattcggtac gcgttttgag ctgtataaag agttcgaaaa ggtgggaacc
     9241 atgcagaaaa tattcaattg gcccggtgac ttttattata ttcgtagact tcactggaca
     9301 gtccaaggtg atatctataa tcatcaatat caaatccatc atttcaatca gcaaatcatg
     9361 aaaatggata aagcaacact tttttcagga gattactatg tattggatgt ctcaaatgat
     9421 gaagatgctc cagtttgtat ttgtattgct gcggttttag attactggct ctaccgtaaa
     9481 gacaaaagtc gcgataagcc tgtttcttac cgacttaact taagttaaaa agatgtacct
     9541 gaaatgcgga aatacgcatt tcaggtacat ctttttagat attcttttgc atatgatcat
     9601 aaagatgacc actgatcatc cgctgaccga caactttgaa gtcatggcgt tcgtataatt
     9661 ttttagcctt tggatttttt tcatccacac ttagaccgat gacggagcga ttagctttct
     9721 tcgccaattt aggtaatgct tctaataatc gcgaaccgat gccttgtcct ctaaaatctt
     9781 ctgatacact gatagagtcc aaataccatt cgttgggaaa cgtttcttta tcaatgaaca
     9841 aacgtgtaga cgcatctagc tgatatttct ctaagatagg taccagtggc tgatcgatga
     9901 ttgattcttc ttcatctgta taaccgtaag caactcctgc aactttaccg tctatttctt
     9961 ctacgatacc tcgtgaataa ctgtaacgat aatttggatc agtgattgct tcttctaaaa
    10021 tctctaaagt cttttgttcc cctgtctgct tcaaaaatgg cagttccata tcttttagaa
    10081 tcactaagat caatggggcg atttctggag catcttcttt tctcgcaaaa cgaatcatag
    10141 tttcctctcc ttttctccat tctaacatgc agaaaaagaa atcgcaaaat cgttatttta
    10201 aaaaatcttc aatcttttca gtaatgaaat aaccatgagt aacgattctt gccgcttcag
    10261 ttcggcaata agggcagacg actggcattt ctttatcatt atgttcgact acattattga
    10321 cttcttcgta tgcaaatttt tttccgcaat tagggcattt cattttttcc atgttatctc
    10381 aacttccttt catctttact ctcttagctt actcttatat tgtaaaaaag agaagcaata
    10441 tccttcttct atgcagatat ccatacctga cggtcctgtt ctccatctat taatcggact
    10501 ttataataag gacgttcatc tttgactaga agtgtccact ctttcacttt tgcttttttc
    10561 ccgtagtagc tgactgcttc cttcatcgct gcttgcggtt gtttgaactc ttccgttttt
    10621 tctatatcaa aaagatgttc tgcggccttt tccgtgtcat ttcgtacggt tgttttccca
    10681 gtggatggat tgactgctat gacttgtcct tcacaaaaaa actggtattc atatgttgca
    10741 ttcgtaggat tctccaaatc tttttcaact gcaaaatgaa tagcagatat gttatgttct
    10801 tttgtttcgt tttttacgat tgctgctgct tttttcaatg agatccgata ttcctggtca
    10861 ctaatatcat ccatgtgaaa agcggcattg agcagtcccg tcataccggt aatccctatt
    10921 acgatgaggg cagcaacacc aaacatcatt tttttcattc ttcattctcc tgtttctttt
    10981 ttttaaagaa tatcaagaaa tgaacgaatg aaaaatcgaa tgaaacgaaa aaaaagtgac
    11041 aattttgtta tctttattcg tgcagttcta atggcaatcc gtctggatca aagaaaaacg
    11101 tcattttttt tcctgtaaag gtatcttctc taatcggttc tgattcgatc cctaaggact
    11161 gcaattctgc aattgtttct tcaatatcgt ctactttgaa agcaagatga cgcaagccac
    11221 aagcttctgg atagtttggt cgttgtggcg cattagcaat tccaaaaatc tccaattcag
    11281 cacttcctag tttcagatct aatttatagt catctcgttc tttgcggtaa ttttctctga
    11341 ttacttcaaa tcctaattta ttaacataaa aatcacgtga tttctgataa tctgaaacaa
    11401 tgatggctat atgatggatc gttgtaaggt tcatatgagc actcctattc ttcgtttaag
    11461 ctcggatcta tattaatctc cagttccatt gtaaatgtgt cagattgaaa aaaatagctg
    11521 tttttccttt ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc caagacactc
    11581 ttcctgttcc caatataata aatgaaagta agaaattata ttttcaagaa aacaaacaga
    11641 aaattatcat aacacaaatt atttataatt gaaagactag ttttttttca agaaaagcat
    11701 acaatagatt gtgttagtaa atacagaaca cactacattt agtgtcttaa ggagtcctca
    11761 tacatgaagt catattttca gtttttagct catcagctaa aaggctggcc gcaacaaaat
    11821 tattatcttt tctttttcag tcttggctgc caggtcatga ctcttgtcaa tagtcccatc
    11881 acttggctgt ccgttattac atttatcggt accacgcttg gtgtattgtg tattttatcc
    11941 atcaatgcgg caaaatcagt caacggtatt ttaggtattt tgtctgcgat ttgttttatt
    12001 atcgttgggt tttcagcaaa gaactattta agtatcggtg aacagattgc ttatatgatc
    12061 acattggata tccccgtatt gttaagtaaa gaatggaatc ataatatggc ttcaaaaatc
    12121 cgaaaattca ctggaaaaag ctggtttatc gcaatcatca gtactttcct tgtctatctt
    12181 gtttcaggat tcctgattgg tcgttataca gatgatcctc ggccgtgggt agatgccatt
    12241 gctttttcta ttagcttgac tgctggtatc atcagtttct tgcgttacaa caaccaatat
    12301 tactggtggt tagcttcagg gcttgctcaa atggtccttt ggtatatttc atttacgcat
    12361 ggatctgcaa gtttggcgat gttcgtgaac agctctgttt acttgctgaa tgatgtcctt
    12421 gcatttaccg tttcaccttg gtataaccaa aaagaacgtg aacgtatgca aaaaatggaa
    12481 gaagaatact acaacgcaca acaacacgca taaaaaaact gagcgtggga caaaagtaaa
    12541 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaatcagaag caactccttc
    12601 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
    12661 cttggagtta aacgcttctg tcccagcctc tattttttat agtaattcct tcagatactt
    12721 tcttgcctca tcattgaaac gcagtgattc ttctaacttc tgataatatt ccaacgcttg
    12781 tttctgagct tcagtaaaac gataatgttt tcctttccat ttgtcgattg tctcttgcac
    12841 atttccagat tggatatctt tcactactag ttgccatttt tcttttgaga ctgtacggtc
    12901 tttcttatca gcagattgta tagcttctcg ttctttacgc aatactttta aaaaaccatt
    12961 tctgactatc cgaggaatgc tgtcgaagaa cccttctgta aaaccatagc gacaatattg
    13021 cttccaaata aatagataga tttttggttg gatgaatcgt tggtttttcg caaaatattc
    13081 catctgagca gtgacttctg gtgttcgttg acgttttgtc tttctcaaca cggaaataaa
    13141 tgtttcaaaa taagcattag gatgtactgg cacttcttca tatacgatgg attgccaatc
    13201 tttacgtaaa aatgcgcgat agttgatcaa gaattcttct acatccttta ctaattcttc
    13261 tgcaaagtcg tcaaatagtt ctttttttat ctgtcgcttt tttgactgcc cattgatgtg
    13321 ttcacttatt ttcttgattt cattttccaa tgcttgataa tccgctatgt aaatcggcat
    13381 ttctacttgg cgaaattctg cgagccgctg agctaatttt atatccggta aatatggctg
    13441 tttcatccga cgattttggt acaagacaaa acagtatttt tgccatagtc cttcaggaaa
    13501 atcgatattc ttctgcttaa gccataataa ttcgtctaag gccagatcaa cttttgtcat
    13561 tccctgatgg atcgaagctg ccactgtcgg agagacatgc aggtgatcgg caaagtggtt
    13621 gatgcctagt tttaggatct ttccggtttt tggcgattta acgatgtatt gcacttttaa
    13681 ttctctacca cactcacaat acaatttttg ctggcttcgt tttgttcgaa aaaacggatt
    13741 gatcttgaag tcagaaaaca cccaatctgt tgcagctaaa taatgttctt ccataaaacg
    13801 agagctgatt agatatcggc ggtgcttatc gactagttcc cgctgttttt tcgttaattt
    13861 tgcatagatc tcccaacgtt gactgcttgt gtattgttta gaaattttga tgggatagct
    13921 tttgctgatt tcttttggaa accattcttc aaagacggat tgccattctt ccacggctgt
    13981 ttctttcctt tctaattaca aaaagctggc tactaacata acgttagcaa ccagccagtg
    14041 tttcttgcta aattagtgag ttgttccggc atttccggaa atatcttctt tattttctac
    14101 tgctgcttca actgcataag tcaaagaaga agcaatgtct tctaaagaca tagaaggcgt
    14161 acccactgga cgatcgatca cttgatcggg tgagaatggc acgtggatga acccacctcg
    14221 aattgccggg aattgtgttt cgatcaaatg aagcaaacga tacatgatat cattgcagac
    14281 aaatgttcct gctgtataag agatgtacgc tggtagacca tgatcttgta cattttttgt
    14341 cattgctttg attggcaatg aggtaaagta agcggctgga ccgttttctt ctaaagccgt
    14401 atcgaatggc tgatttcctt cattatcagg gattcttgct tctgctagat tgattgcgac
    14461 acgttcgaac gagactgctg accgcccacc tgcttgtccg acacagatca cgacatctgg
    14521 ttggtgctgt tggatggctt ctttgactac ttgactgctt ttttcgaata cggtcggtac
    14581 ttccactttg atgatttcag caccgctgat ttcatctggc atttttttta ctgcttcata
    14641 ggctggattc actttgtccc caccaaatgg atcaaaacct gttactagta ctttcatctg
    14701 ttgttcctcc gtccgattga tttaacatat ggttatcttt tatcatacca ttttccttga
    14761 acagcgtaaa taaaggaaac aatagtttaa ataacataac agatcaaact taatatattc
    14821 ttataaaaaa atagtgttcc actacaatgt tagaagaggt tgcgacaatt attctgtcac
    14881 aacccctctc ttctattatt taaatagaat catataagca atttggaaaa ctagcataaa
    14941 taacgcaata aacaactggt ttttgatcac tccgtatttg tctttcatct ccagcatcgc
    15001 tacaggcacg atattgaagt tagcagccat cggtgtcagc agggtcccac aaaaaccgca
    15061 agtcaatgca accattccta ttaacgctgg attcgctcca tgactaaata cgaatggtgc
    15121 accgatccca accgtcatca cggtgatggc cgcaaatgca ttccccatga ttaccgtaaa
    15181 tatcaccatg ccaagagcgt aaatgatgat gccgatgttc acatttcctt ctggaacgat
    15241 cgtacctact gcattggaaa tcactgtccc aactccagcg cttgtaaata ccgcacctag
    15301 agaagctaaa agcattggca acatgcttaa cggccctact gtgcccagca tatctgctgc
    15361 atcatctaaa aatgtacttg gtcgattatc tcgagaaaag aacatcaaga ttagcatggc
    15421 tgcaaaaaca ccgacgccca cgccgactaa agcacctaaa ttggtaaata gagcaaacaa
    15481 gatcgcaaag acaccgatac tcaaagctgg aataaaaatt ttcattccta gtttgtctga
    15541 cattttttcc atatattctt ttgatggaag ccggctttct cctttgctga ctttcttagc
    15601 aattgctggt atggtcatcg caaagatcaa gccaccgttc acgattgccg gaagccatct
    15661 tcctcctgca ataactaccc ctaagaccgt ccagaaaaag gctgacccat aacgatgagg
    15721 attggtttta tctgttaaat ttcttactcc agtgtaaatc aatacgagtc ccatcacgat
    15781 ataaaggatc tccagtaatt tatcgcctaa taaaatatca ggatttgtaa aaaagtccat
    15841 cttattgccc ctttcctgga taatatttct tcgctaattt cttttcttta agatagaagt
    15901 agacacttgc cacaactaaa gccgtgatag ccactggaac ttccactaaa gcaagctgtc
    15961 ccaatgttac gtcatatcct agatctttca gtgttgattg gactagcaac gcccctgctc
    16021 ctccgatgaa tagtacttgg ccaaagaacc agcagacatt ttccatagct gaagccatac
    16081 cttttagttc ttcttcatgc ttaggatgga cttctaaccc ttttgattcg atggtcccca
    16141 atgccatcgg taaaatgatt ggacgaacaa atccagcaac tccaccaaaa ctcacgttaa
    16201 aagccgcaaa gatcattcga aagatcccat aaatatctac aatcatacca gcagatactt
    16261 tcttgaatcg cttgatcaag gaagccgctg attctttcag accatttctt tcaagcgttc
    16321 ctgttgccaa cataatgatg ataaaaatcg ccatcccgcg attgtttaca aaacttgtac
    16381 ctaaaagttc taacattcct tgaatgccaa gaccgccagt caaagctgta acaactaaag
    16441 caaccattac gatcaatatc gagtctaatt taaatgcaaa cccgagaata acaatcaaga
    16501 cgcctaaaag gctaagataa ttttccatat attttcctcc ataaaatgac aatatttttt
    16561 taattataaa gcaaaacaca taa
//
LOCUS       DAZKCZ010000053        16542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000053 DAZKCZ010000000
VERSION     DAZKCZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.053"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..1209
                     /locus_tag="ACG8KN_001597"
     CDS             70..1209
                     /locus_tag="ACG8KN_001597"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFP9188657.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1202..2002
                     /locus_tag="ACG8KN_001598"
     CDS             1202..2002
                     /locus_tag="ACG8KN_001598"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9188658.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            2002..2829
                     /locus_tag="ACG8KN_001599"
     CDS             2002..2829
                     /locus_tag="ACG8KN_001599"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9188659.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2834..3541
                     /locus_tag="ACG8KN_001600"
     CDS             2834..3541
                     /locus_tag="ACG8KN_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9188660.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3641..4507
                     /gene="rfbA"
                     /locus_tag="ACG8KN_001601"
     CDS             3641..4507
                     /gene="rfbA"
                     /locus_tag="ACG8KN_001601"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9188661.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4521..5093
                     /gene="rfbC"
                     /locus_tag="ACG8KN_001602"
     CDS             4521..5093
                     /gene="rfbC"
                     /locus_tag="ACG8KN_001602"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFP9188662.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5115..6143
                     /gene="rfbB"
                     /locus_tag="ACG8KN_001603"
     CDS             5115..6143
                     /gene="rfbB"
                     /locus_tag="ACG8KN_001603"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9188663.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHSEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6241..7092
                     /gene="rfbD"
                     /locus_tag="ACG8KN_001604"
     CDS             6241..7092
                     /gene="rfbD"
                     /locus_tag="ACG8KN_001604"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFP9188664.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7127..9160
                     /locus_tag="ACG8KN_001605"
     CDS             7127..9160
                     /locus_tag="ACG8KN_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188665.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9204..10484
                     /locus_tag="ACG8KN_001606"
     CDS             9204..10484
                     /locus_tag="ACG8KN_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFP9188666.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10694..11500
                     /locus_tag="ACG8KN_001607"
     CDS             10694..11500
                     /locus_tag="ACG8KN_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188667.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11512..12732
                     /locus_tag="ACG8KN_001608"
     CDS             11512..12732
                     /locus_tag="ACG8KN_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188668.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12722..14308
                     /locus_tag="ACG8KN_001609"
     CDS             12722..14308
                     /locus_tag="ACG8KN_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9188669.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14347..16485
                     /locus_tag="ACG8KN_001610"
     CDS             14347..16485
                     /locus_tag="ACG8KN_001610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9188670.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         5245 a         2733 c         3512 g         5052 t
ORIGIN      
        1 gagttcttat tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta
       61 ggtgcccata tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta
      121 tcactgatat taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa
      181 ccaaatgaaa gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt
      241 atttcatttg ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt
      301 gtaccgatct tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat
      361 gagctgacac ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt
      421 gtagcagata tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc
      481 tggcttagtt ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg
      541 attgacggtt tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga
      601 atcattggat attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt
      661 cttgtagcaa gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta
      721 ggcgatacag gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg
      781 aagaacgtga cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca
      841 gacacgtttt ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat
      901 aaaaaacatc ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg
      961 acgatttatg cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga
     1021 agaatcggaa cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa
     1081 ttgattggtc tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg
     1141 aataaagagt accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata
     1201 gatgaataac aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa
     1261 aatttttcgg acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat
     1321 ccgaattttt gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa
     1381 atatccctac atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa
     1441 tcacaatctg atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt
     1501 ggatgaagag acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat
     1561 gttagcacct aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct
     1621 ggctgttttt gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga
     1681 caaacgactg gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg
     1741 gatgatctca ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt
     1801 tgatgagcgt ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc
     1861 aggttacaag ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc
     1921 gcatcgaaat gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa
     1981 gtgggggtgg cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag
     2041 caactattca gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa
     2101 attcagctta ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa
     2161 gaaaatatcg tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt
     2221 gcgttgtctc aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg
     2281 ctagtcgacg attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct
     2341 gcagttccga tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat
     2401 atcaatcgta cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca
     2461 atcaattcag gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct
     2521 gctttttctt tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa
     2581 actgtggaga tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa
     2641 gtttcaagta atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat
     2701 gatcaaaggc atttatccag tcaccgacga caattattgt taagaacagc taaacagttc
     2761 ttgaccgtca aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg
     2821 aaggtgtgaa ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag
     2881 agagcagtta gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga
     2941 tgatgggtca gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg
     3001 aatccagctt ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca
     3061 gcagacgaaa ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa
     3121 agtgaaaaag atcactgact attttgagca gaatccggat cagctagtag tagtttctga
     3181 tttaatcatt gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa
     3241 ttccaaagaa ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc
     3301 tttccggtct tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga
     3361 tatgtggatt ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct
     3421 gtaccgaaga catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa
     3481 aatttcttgg cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg
     3541 ataaaagatg tttaaatcct taatatttcc atgacaaata taacaattga tgagataata
     3601 acaaagtgaa atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc
     3661 tgggggcagc ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc
     3721 gatttatgac aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa
     3781 agaaattctg attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa
     3841 cggaaatgat ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc
     3901 acaagcgttt atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg
     3961 ggacaatatt tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga
     4021 tacaggcgcg acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga
     4081 atttgatgag aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa
     4141 ttacgctgta actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat
     4201 caaaccttca gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa
     4261 aggaaaacta tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca
     4321 tgaatcattg cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa
     4381 agtggcttgt ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct
     4441 ggcccttgca cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc
     4501 tgaataagga gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg
     4561 tagaaatgga cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa
     4621 aatttgcaga acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg
     4681 aagctggtgt ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga
     4741 ttcgggtagt cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga
     4801 cttatggtca atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac
     4861 ctaaaggata tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt
     4921 gtgataatta ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag
     4981 cgattgattg gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc
     5041 cgacattaaa agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa
     5101 ggagaaattt tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta
     5161 atttcgttca ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac
     5221 tgacttatgc aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc gtagaattgg
     5281 tcgtaggaga tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag
     5341 ttgttcatta cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg
     5401 tacaaacgaa tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac
     5461 gctatcatca cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc
     5521 caggacatgg ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa
     5581 gtccatattc ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt
     5641 ttggcttacg tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatagtg
     5701 aaaaatttat tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca aaattatatg
     5761 gcgaaggaaa gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc
     5821 tgattttgac gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag
     5881 ataataagac cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg
     5941 accatgtaaa cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg acaaaattgc
     6001 gagaagagct aggttggcag cctgagttta cgaacttccg tgatgggttg gctgacacga
     6061 tcaaatggta cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag
     6121 catatgccaa aaatggacaa taatacagag gaaatttcac tgtattaatg gaaaaacaca
     6181 tgtgaggcag gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataaccat
     6241 atgtttttat taactggagg aaatggacaa ttaggtacag aattgcgcca tttattagat
     6301 gaaaaaaatc tgccatatgt ttcaacagat gcaaaagact tggatatcac agatgctgag
     6361 aaaacaatgg cttatattac agaattaaaa cccgatgtta ttttccattg cgcagcttac
     6421 acagcagtag ataaagcaga agaagaagca aaagaattag atgagaaaat caatgtcgat
     6481 ggcacaagaa atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt
     6541 acagactatg ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg
     6601 aatccactaa atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt
     6661 ttagaagact attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc
     6721 gtcttcacga tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag
     6781 tttgggcgac caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa
     6841 aaagcaccat ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgagttc
     6901 gcaaaagaaa tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt
     6961 ccgcaaaaag caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagcgtta
     7021 ggttttgtca taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa
     7081 atgaataagt aatttccttt ttatcaaata gaatttggag catgatatgg aaaaacaaac
     7141 aagaagtgca cgacgaaaag aaaagccaaa agcgaatcag gcagattcga atttggtgct
     7201 tgacctgttt ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg tcatcgttac
     7261 ttttaattta aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc aaagagacga
     7321 tgggaaaaaa actgatgtga tttttggaca gaatcgggaa gtgcggtcag acgaatggct
     7381 ggtgcagaca cctttttacc tctcacaagc tgaagaggac ttttcgttgg tgaacgagga
     7441 ttattcatta aacggacaaa acatgatcat tgcgtataat tctcctgtaa aggatattac
     7501 ggttatcgga aaaccattca actggggatt ctttttcctc ggaagagatc gaggactttc
     7561 cttttattgg gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag tactgatgat
     7621 attaacgaag aggaaaaaag ctctatcgct aatcggtgca tttgcgctga cattttctcc
     7681 agcagtccag tggtggttca tgcaacatgt gggcgatttg attttcttta ctttgggact
     7741 gatgattgct ttttatcatt atttctacaa tcatgataaa aaatggctac gaacgttgat
     7801 gatgcttttg gtggttattt tcggtattgg gtttatttta gtgatttacc cagcccatca
     7861 agtgatgctg gcgtacttgc tgatttttta ttttgttggc ctgcttattt actatagcaa
     7921 gaaagtttca tgggatctgt tggatgtagt gttgattgga ggggcagttt tatttattgg
     7981 cggagtcatg gttcatttct ggctaacgtc aaaagacgca ttgttagctt ctttgaatac
     8041 cttatatcca ggaaaacggg taagtactgg aggaaagtgg acgattggcg aatttttttc
     8101 tttccttaca aattggaaaa ttccttttga agatatcaac tattcgaata actctgaagt
     8161 ggccctgttc tatcactttt ttccaactgt attcctagca agtccatttg tgttatttgg
     8221 caaaaaagat agcgaacaaa agttactggg acgtattttg atgatttttt gtctgttttc
     8281 tatcttttgg attaccgttg ggttgccaaa atggctagca gaaatcactc tgctgtctta
     8341 tgttcctcca ttaagagcca tccttacttt tagttttgcg gcttgtttat tatcgatctg
     8401 gtttatcgct tatatttgga gagaagatgt tattcctatt tggtataaat tcattttgtt
     8461 ggcagtaaat gcatggtctt atttctatgc gattacgcat tcgaaaatgg ataattattt
     8521 ttcggatttt gaaaccatcg cagttgctgt gttgggaaca gtgattttag cttttgcatt
     8581 atttggctgg agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg tatctggctt
     8641 tttcgtcaat ccagtcgtag aaggtacagg agcgattttt gaaaaaacac tggcaaaaga
     8701 aatccaaaaa atcgatcaac aagatcctgg taaagtttgg ttgtctgaag atgaattata
     8761 taacttcacc ccaacattgg gagtgaaatc cttcaataca gtacgctttt atccagatat
     8821 agctgcatgg aagaaaatcg atccgaaaga agaatacgag aagtattata atcggtatgc
     8881 tcatacaaga gcttttatcg ctaaggaaga tacaaagttc attttagatc gtccagatat
     8941 gttttcggtc acgttgaatt ttgaagacgc agaaaaatta gggatcgact atgtgatcag
     9001 taaacgacca ttaactgatt acaaccaatt atatgaagcg cagttcacgc agctatatgg
     9061 accggataaa gacggttata tgatttttaa agtagaatat ccggattatc cagatgtacc
     9121 acaaacacaa actcaggaaa atcagctagt cgtacagtaa taggctagct gagaataatc
     9181 ctgaaatatt agtagaaagg agagtgaaga taattcaagc aatcgtacaa aaatttgtca
     9241 acaaaatgag tgtcggtttc ttgcttttgt tgctgacgct agtctttttt gtttggacag
     9301 caggggtagg ttttccagca actttgttta tatataacaa tagttcatta cttatcgctt
     9361 ttagtgtggc tattttgctc attttaaatg tttataaagc aaaaggcgtc gattttcttt
     9421 atctaggtgt tgcgcttgcc cttttcattt tcttttctta ctttcgggaa ttaagaaatg
     9481 agtctttatt tggagactta ctgtttcctt tgattatttt attttgttta gtaattaaat
     9541 ggatcgagtg gacaaaatgg gatcgcggta tttatctgat tgtctttacg gtcttcctcg
     9601 gaatcacgtt atatcgtgta tttacagaga tccaagtacc agaaggacaa agcatctggg
     9661 ctccaggcaa ttcgctttct gatatttgga tcaatacgaa tacaatcgga agttctttga
     9721 tgactcttgc tttattgatc agtggatttg ccagttcttt tgaacgatgg tatgttcgaa
     9781 tcatcggtgg gatagcagtc gttgcagcct ttttaggtat ttgggtctgc caatctagag
     9841 gtgcattagt cgcgttgatt gtttttgcga tattggatct tttacctaaa tcgttgatgc
     9901 gtgtcattcg tgcgccattt attgcctaca cggttacgat tcttttagct ttgccgattt
     9961 cttatctggc ggctgtctct gaaaaagtca atttgtttac cggtcgagaa gatatttggc
    10021 ataaattcta tcagacgcta ggagaaaaat ctgaacaagt tttactagga atgaaaacat
    10081 ttatcttcca gcgaggaaac caatttttag gaaaccacaa tagttataat tctattttga
    10141 atatctatgg attgatagga tttgggattg ctgctttgct gcttatcctt tttattggtc
    10201 gcttaacgtt gaaagcagat ttatccaacg ggcagatgac ttttatctgg gccttttttg
    10261 cggtgatgat gcagtcattt atggaggata cattgacttc atacccatgg gttccgatta
    10321 tttatatcct gttggctatg gccgttcatc gatatgatga accagaagtt acggaggaat
    10381 tctatgttat ggaagaggca tatgacgata tgacttatga tgatacatct tatgaggaac
    10441 aaccagatag cgctcattct tccaggatcc aacgctatca ttgataggca caaaatacga
    10501 gagaggctgt gacaaccgtc gcagcctttt ttggtttatt gcaattgtct agcatcttct
    10561 gtaaaataga aacgttagag aaaatcttgt atcatagaaa attaagaaag aaataagaac
    10621 gcagctcggt accaaaaata tggtaaaatg gcacttgact gtaattcact gaaaatcgct
    10681 tggaggaaca caaatgttta aagatttgta tttatttatg aaggacattt atcagaacag
    10741 aaaattactg ctacagtttt cgatcaatga cttcaaatca agatatgcag gatcgttttt
    10801 aggaatctta tgggcgttta tcaatccgct atttactgta ttgatttatt ggctggtatt
    10861 tggtgttgga ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg tatatttagt
    10921 taccgggatg attccctggt tcttcttctc agatgcattt gcatcaacta cggttgtttt
    10981 tcgggaatat acttatttag taaagaaagt cgtattcaat attcgtatct tgcccacgac
    11041 gaaaatccta tccaatctgt acacccattt attcttcatt ctgattggca tagctgtcgc
    11101 gcttataaat ggtatatatc cttcagcaat gtctttacag ctgatttatt atttattttg
    11161 catgtgtgct ttcttgacag gattgacttg gctgacggca tcgatccaac catttttacc
    11221 tgatatcatg caattaatta cgatcgtgct gcagttgatc atgtggacat taccgatatt
    11281 atggagccct aatcagtttg attcaggtct tgtaagcata ttgaaattaa atccgctgta
    11341 ctatgttgtt caaggttatc gtgagtcatt tttaagtgaa gcttggttct gggagcatgg
    11401 aacatatact ttgtatttct gggttttcac gttagtaatg ttcgtaatcg gctctgcagt
    11461 gttccgtaga ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc tatgacagaa
    11521 tatgcaatta gactaaaaga tatcacaaaa tcttataata tgtacgcaaa accttcggat
    11581 cgatttcgtg aagccctcaa tccttttaag aaatcctatc atgatgtatt ttatgcgttg
    11641 aaaaacgtga atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga
    11701 tcgggaaaat ctacaatgtt gaaaatcatt acgggagtac tgacaccaac atccggcacg
    11761 atggaaatcg aaggaaaaat ctcagctttg ctagaactag gatcaggatt caatcctgag
    11821 tattctggct atgaaaatat ctatttaaat ggaatggttc taggattttc aagagaagaa
    11881 gtcgatgaaa tggtcgatga tatcattgaa tttgctgaca tcggcgacca tatttatcaa
    11941 cctgtaaaaa cctattcaag tgggatgttc gttcgtctag cttttgctgt agcgatcaat
    12001 gtgaatcctg atattttgat tgtggatgaa gcattggcag taggagatct tgaatttcaa
    12061 ctaaaatgta tggaaaaatt tacagaaatc aagaactcgg gaaagacgat tttattcgtt
    12121 tctcatgata ttagttcgat tcgccgtttt tgtgatcgtt cctattggtt gaaaaatgga
    12181 aaagttgtcg aatatggtga tacgatggaa gtaacgacga attacgaaaa ctttttgaag
    12241 aaaaaatcag ttaaaacagt agatcgaaat aaaaaccaag tagaacgctt cgcagctcca
    12301 gatatcgtag acgtgacgaa agccgaattg ttggataaag ataaaaatcc agtggatatg
    12361 ctggaacaag gagaaaaact atacgttaaa gtaacctatg aagtaaaaga cgatacaatc
    12421 aaaaatccag ttttaggaat tgctctgcgt acagtcgaca atttttatgt gtgcggactc
    12481 aatactctac tagatggtat caagatccct tggaaaaaag gtgagaatat cttttatctg
    12541 gagtatgaaa agttaggttt gttagctggt gaatattatt ttgacgttgc ggtctttgaa
    12601 gaaaatgcaa cggttccttt ggtatacaag acaaaataca tgaacttgtt tgtcagcggt
    12661 tcttatatcg gagaaggcat cgtggtctta gaccataaat gggaagaagg gactcatagc
    12721 aatgaaatat gattttgaaa tggacctaga tgaacaaagc agtgtgggaa aaattgccgc
    12781 acagatcaaa ccaggaagta aagttttaga gtttggtcct ggaaacggcc gattaacgaa
    12841 acatttgatc ggagcaaaac aatgtgaagt gagcattgtc gagctagata aggaattgtt
    12901 cgattttgtc agcgagtttg cccaagatgg attttatgga gatatcgaaa gttttgaatg
    12961 ggcaaactat tatgctggac aaacatttga ttacgttttg tttgccgatg ttttagaaca
    13021 tttggtagat cctggaaaaa cgttgaagaa agttagagaa tttttgaatg aagaaggaga
    13081 aattctaatt acattcccta accttgcgca taattctgtc atgattgatt tgttcaataa
    13141 tcaattgcca tgggcttctt atggtttgct agatgaaacc cataattctt tttatactca
    13201 tgatggcttc caaaaagttt ttgaaaaagc aggattatac atcaatattg aagattattt
    13261 gtacttagct gtaggtgata cagaattaaa atcaacttat gaagaattgc cggaagctgt
    13321 acgttatgat ttcaaaatgc gaccatttgg cgaagtctac caatacttct tctcattgat
    13381 gaaacatcca gtagcacaaa gttccattgc gcagccgcaa aattctaatt atgtgaaagt
    13441 attagaagta atccagcaaa cgaaacaaga tgaaataatt caacaaatac cattcaataa
    13501 tttcacaggg gaaaatgaga cactaagttt ccatgtatca gaattagcag aacgaatggt
    13561 gtttcgcttc gcgcagcaac ctagctttat cgaattcagc gcagaagctg ctggagaaaa
    13621 actcacattt atcgattcaa atgctgtagt caaaacagta aatgattgtt atttgtttga
    13681 tggtaaagat ttaccagagt tctttttaac ggatattgca gggaaagaag tgactatcca
    13741 ttgtcactat cgttttatcg gagaattgac accaacaatg aaagaattgc tggaaacaat
    13801 ccgtccaatg gctgaagtta caaagcagct ttctgagaaa aatgatgaat tagaaagaga
    13861 aaatcattat cttattgaga ataatacaag attagatcag acgttgaaaa caaccataaa
    13921 tcgctattgc acgttgttgg aaagtgatga atggacaatc aaacatagat tagggcgacg
    13981 taagaaagaa acatcgaaaa aaatccaaga aaaagagctt tctctatgta ttgacgaaaa
    14041 aatctgggat gcagaaacaa agatattgaa aatcatcggg tggggaatcg ctaattcgga
    14101 tcgccaacca ttgtcctata agttatcagc cgaccaatca ccattttttg aagcaattcc
    14161 tgttttacga gaagaagtca atcaagcaaa acagctagct gaaggagcaa aagcaggatt
    14221 tgaactgcgg atcctttgcg agaaggaacg ttcattctta gttgaagcag tggctcaaaa
    14281 tggtcaatca tggatcattg aaatgtaaat aatgattaac ttataaagat aaggagtaaa
    14341 cgaatcgtgc aaaaaaaaga aattgccgta tttatcgatc aaatcactcg tgaacgggcc
    14401 actggcgatt tgcttattgt gggctgggca attgatgaag tgaccaaaga aatccctacg
    14461 ataaaggtag aaaaagaaaa tgtgattgct gaagcaacac atgtcgtaag attggatatc
    14521 aatcatttat ataatcttga tgttaagacc caaagcggtt ttaaggtacg tttatcaggg
    14581 aaaatgagag gaaaagcaat cttagacttc caaacagcaa gacatcaaaa tggcatagct
    14641 gtgaaattaa acggtaaata tccttatgat gacggcattg aatcttcatg ggaaagaaaa
    14701 aaaagattac tgaaaaaagg gataaattat gcaaggactc atggcgtaaa aaaagcaatc
    14761 cgccgggtca aactggaatt gaaaccagga tcgatcgact atgcagagtg gatcagtcgt
    14821 catgagaaac ctgatctgaa agagcaaagg aaagaagtac aaggatttgc ctaccgccca
    14881 ttgatttcca tacttatgcc tgtctataac gttgaaatca aatggctcga aaaatgcatt
    14941 gattcggtac ttgatcaaac gtatgatcac tgggaactat gtatcagtga tgatgcttct
    15001 actgatccag ctatccgaaa gtgtctagaa tcctatcaag caaaagatga tagaattaaa
    15061 gtcgttttcc gacaagaaaa tggacacatc agtctggcaa ctaattctgc attgaaaatg
    15121 gcagaaggag agtttatagc gctcttagat aatgatgacg aattacctcc atttgcctta
    15181 tatgaagtgg caaaagtgct aaacgtacat ccagaactgg atttgatcta cagtgacgaa
    15241 gataaaatcg atgcagatgg gaatcgcttt gacccgcact tcaaagcaga ctggtctccc
    15301 gatacattga tgggaaataa ttatatctct cacttaggcg tatatcggac aagcattgtt
    15361 aaggaacttg gcggtttccg taaaggatat gaaggctctc aagattatga tttggttctt
    15421 cgtgtgacag aacaaattcc tgcagaacac atttaccaca ttgaccgtgt cttgtatcat
    15481 tggcggacga tcccgggatc aactgcaagc aatggagaag ctaaaagcta catctatgat
    15541 tctggagtga aagcattgac agatgctcta tctagaagaa atatcaaggg aagcgtacat
    15601 cctggcagaa tttctggttt ttatgaaatc acttatgatg ttttgcaaga agacttggtc
    15661 agtgtgatca ttccaactaa gaatggctat gaagatctaa aaacttgtgt ggattcgatc
    15721 atcgaaaaaa cgtcttatcc aaattatgaa atcattattg cagataatgg aagtacagat
    15781 cctaaaatgc aagaattatt tgcagaatac aagcatcagc tgaaagaccg atttatcgtc
    15841 gaattaattg acattccatt caattattca cgtatcaaca atctggcagc agaaaaagca
    15901 aacggcaaat actttttatt cttgaataac gatacagaag tgattgaacc tgactggatg
    15961 acagcgatgg tttcttatgc acagtttgat cgaatcggct gtgtaggagc aaaattattt
    16021 tatccggatg atacaacgca gcatgcaggt gtattattag ggatcggtgg cgttgcagga
    16081 catgcattga ataactatga tcgaacacat tgtggttact ttggccgatt ggtcattgac
    16141 gtcaattact tagcagtcac tgcagcttgt atgatggtca aggcagctga cttcaatgct
    16201 gtcaatggat ttgatgaaat attagaagta gcattcaacg atgttgactt gtgcttgaaa
    16261 gtttatgaac tgggtcgtta caatgtctat gcacatcaag cagaattgta ccatttcgaa
    16321 tctaaatcaa gaggttatga agatacacca gaaaaacaaa gacgttttgc tggggaaatc
    16381 aaaaaaatgc aagataagtg gccttcttat attgctcatg acccatttta taacgataat
    16441 ctaactaaag aaggaatcgg cgatttttcg ttgagacctg attgattaaa agaactgtat
    16501 tttcattttt attaatgaaa atacagttct tttaatttaa tt
//
LOCUS       DAZKCZ010000054        16541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000054 DAZKCZ010000000
VERSION     DAZKCZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.054"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..2151)
                     /locus_tag="ACG8KN_001611"
     CDS             complement(112..2151)
                     /locus_tag="ACG8KN_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9188671.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGATVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGFDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2209..2964)
                     /locus_tag="ACG8KN_001612"
     CDS             complement(2209..2964)
                     /locus_tag="ACG8KN_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9188672.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            complement(3056..4162)
                     /locus_tag="ACG8KN_001613"
     CDS             complement(3056..4162)
                     /locus_tag="ACG8KN_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9188673.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     regulatory      complement(4336..4502)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(4727..4933)
                     /locus_tag="ACG8KN_001614"
     CDS             complement(4727..4933)
                     /locus_tag="ACG8KN_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188674.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(4949..5563)
                     /locus_tag="ACG8KN_001615"
     CDS             complement(4949..5563)
                     /locus_tag="ACG8KN_001615"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9188675.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(5710..6393)
                     /locus_tag="ACG8KN_001616"
     CDS             complement(5710..6393)
                     /locus_tag="ACG8KN_001616"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9188676.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(6502..6960)
                     /locus_tag="ACG8KN_001617"
     CDS             complement(6502..6960)
                     /locus_tag="ACG8KN_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9188677.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(7044..8807)
                     /locus_tag="ACG8KN_001618"
     CDS             complement(7044..8807)
                     /locus_tag="ACG8KN_001618"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9188678.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9028..10488)
                     /locus_tag="ACG8KN_001619"
     CDS             complement(9028..10488)
                     /locus_tag="ACG8KN_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188679.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(10696..11622)
                     /gene="glsA"
                     /locus_tag="ACG8KN_001620"
     CDS             complement(10696..11622)
                     /gene="glsA"
                     /locus_tag="ACG8KN_001620"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9188680.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(11645..13072)
                     /locus_tag="ACG8KN_001621"
     CDS             complement(11645..13072)
                     /locus_tag="ACG8KN_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9188681.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(13103..14317)
                     /locus_tag="ACG8KN_001622"
     CDS             complement(13103..14317)
                     /locus_tag="ACG8KN_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFP9188682.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(14403..14630)
                     /locus_tag="ACG8KN_001623"
     CDS             complement(14403..14630)
                     /locus_tag="ACG8KN_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188683.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(14658..14900)
                     /locus_tag="ACG8KN_001624"
     CDS             complement(14658..14900)
                     /locus_tag="ACG8KN_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9188684.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(14932..15489)
                     /locus_tag="ACG8KN_001625"
     CDS             complement(14932..15489)
                     /locus_tag="ACG8KN_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9188685.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(15502..15690)
                     /locus_tag="ACG8KN_001626"
     CDS             complement(15502..15690)
                     /locus_tag="ACG8KN_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9188686.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(15703..16269)
                     /gene="amaP"
                     /locus_tag="ACG8KN_001627"
     CDS             complement(15703..16269)
                     /gene="amaP"
                     /locus_tag="ACG8KN_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9188687.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         4820 a         3437 c         2739 g         5545 t
ORIGIN      
        1 tgagtaaatc aaaaagaagt tgtgaaagag acatcgtgaa aaccgtttat ttgaaaaaag
       61 gactgtgacg ataacgccac agtcctttta ctgtacttaa aaagcaattt tttattgata
      121 attttggttg aggtattgca atagttccga tgctcgtttg gttgctgaat catcatcttc
      181 tttattttcc aacatgctga ccatcatata accttggtta tcaagattga aagcaaacaa
      241 gaaactgttc tcttgcccct ttacatcctg tttttctttg atttccgctg taccagtttt
      301 cgctgccaac ggaatcccta aagcagaaag agaatgtgct gtaccattta catcttgcac
      361 aacttctctc agatctggca cgatcgtttg tactgctgtt tcgctgatga cattcttctt
      421 atcttttgtc tctttatctg caatcaattt aggatagaca agtgtgccat tgttggtaaa
      481 aacagaatac attgctgctt gctggatagg agtaattaga agttcgccct gtccatatcc
      541 agtatcagct agcaagatat ctgagttaaa gctatcttca ttagaaattt gtgctggatt
      601 catactaatt ggcaaatcaa ggtcttcacc aaaaatgaat ttatctaagc ctgtacgaaa
      661 ttttttctca cccattttca acgtttcttg tgccgcatat atattatcgg aatagatcaa
      721 agcagttttt aagtctactt gtgatacatc actactgaca cgcgttgttt gaaaagatcc
      781 ccaagaactg tctttttgcc atttaagccc gttgatcgtc aacacttcat ttggatcgat
      841 agtgccgttg tcgaaaccga ttgctgctgt aattatttta aacgtagatc caggagcata
      901 acctgtcgca aatcggctga tgaatggatg ttcaggattt tcttcataag ctttgtaatc
      961 ttcttgtgag atcccgtttg tcattttgtt tggatcatag cttggagagc tagcaagcgc
     1021 aagaagatca ccggtttttg gcgttgtcgc aacagttgct ccagcttttc ctcctagact
     1081 gtcaaaagct gttttttgtg ccttcgcatc gattgtcaat ttgatatctt tgccattttg
     1141 aacttcatgt tcgatcagaa cttttttctc tacaccgtct gcatctgtaa tgcttaattt
     1201 cccacctgtt gtcccacgaa gatccttatc aaaagccatc tccaaacctg agcgaccgat
     1261 ttttccatta ctgcttaatt ctggattctt atcaatatct tctgctgtga tatctcccac
     1321 gtaaccaatc agctgagcag ctgcttcacc caaaggataa tatctgccgt ctacttcttg
     1381 aatggtagct ccagctggaa gttctggcgt tgctccatca atgattttca atgggacaaa
     1441 gtaatcgggt tgtacccagc tttgtgaaat cgcctgattg atagcatctt ctgttaagtc
     1501 gaaggaggaa gcaatcgctt tgatattggc tgttttttcg cctccatctc caagtttact
     1561 tggaacgact cctaattgtt ttagtttgcc tgttgttgct aatggttcac catttcgatc
     1621 tataatattc cctcttgctg cttcttgtgt ggtcaggctt actttgtcat ttccttccat
     1681 ttctggaaaa accaagttgg gttgccagtt gatcgtggtt tgaccatcat ttctgtctaa
     1741 tgttccttta taagaaagat ctttcaattc acctaatgag gtattcatct ttgctttata
     1801 tgaaaaactg taagtagaat cgtctttttt atctactttt agattcgata tctgaagtcc
     1861 tttgacatca gcagcaccgt atatattctg gtatttatct aagatctctt tttcagataa
     1921 tgcactttta tttgccgcct tttttgatgt catttctgca gctttgttat aatctccctt
     1981 gttcaaagct tggacaaatt gctcaactgt cttttctcca gcttctactg cttgggtttc
     2041 ttgatagtgc tggtagtaaa aataaccgcc acccgcagct gctattaaga tcactgcgcc
     2101 ggcaatataa gcgcctgttc gatttttgcc gtgcttgtca cttcttttca ttttttcctc
     2161 cttctcttgc ttgtcattcc tttatttcac tatttatacc tgtttattct aacatatgtc
     2221 tttgaggaaa tccataccac aatccagaag cacactttct ggaaggttcg tttggatact
     2281 accaagtaat ttcccaaccg ctttgggtag cttgaatatt gaagttacgt ctttcagctg
     2341 ctgagagata gctttgatca cttgctgctg ccttctgatc ctcccgaagt ctccttcttt
     2401 gtcttcccga aatcgagcat actggagtaa agtattacca tccatcactt gctctccttt
     2461 gtttatatat acgccatcta aatctagaga tttttctgca tcgatcttga ctccaccagg
     2521 aaacagttca ttaacgcaat cgataaaatc ttgaaatgtg atacttgcat aatagggagc
     2581 ttcaaatttg aaattttcct ttaatgtttg atttaataaa tcaactcccc cataagagta
     2641 agctgcattt atcttattca ttccgtaacc tggaatatcg acatatgaat ctctcatgat
     2701 agagatcagt tttgcttgtt gtgttttctg gttgtattgt gcaaccagaa gtgaatccga
     2761 gcgaccgttt tcgtctttcg aacgagcatc tgaaccaatc aaagccacat ttagtgtggt
     2821 agggatactt ttcggctcta cctttttcac agttgaactc tgttttgcag acgaaaatcc
     2881 atggataaac tcaatcgaaa aatagccaac tactaatgta agcagtaaca aaatagataa
     2941 tatgattttt tgagatactt tcattgcgaa ttcctctttc taaaagaacc tctcgcttga
     3001 aaaataaaag attgtacttt atgataattc aaactgagag tttctcagtt tctctttatt
     3061 gtttttcagg tcgatagagc gccaactctt cggcctgtat ctttgaagaa atattcaacg
     3121 tgattccgat tcctaaagaa aggatcagat aacttgttcc tccgtagcta acaaatggca
     3181 atggcacacc agtcattggc atcagccccg cgatacttcc cacgttcatt atcgtctgaa
     3241 cgaatagcaa agttccgaat cctaaaagaa acaatccagc ttgctgattt ttacagcgac
     3301 tgcttaaaca aaagattctc atgcataatg agaacaataa gaaaagcaca cacaatgcac
     3361 cgattagccc aagctcttct gtaataatcg aaaaaataaa atctgtttct gtctctggaa
     3421 gatacccttt ttttgtgata ctatttccta atccccgccc aaatatgcca ccattatata
     3481 gtgcataaaa cgaatgggtc atttgatagc ccgcaccatg actatcgata aaagaatctc
     3541 ttaaagtcgc gatcctttca taagcatgat taaacatctg tggaagccaa cctttatttc
     3601 ctaaatataa taccccgcct gctgctccaa tcagcaatgc agaaaaagta acgattagat
     3661 agatcccttt tttaaatgga accgccgctg cccaaaatat gacccacgca atcgaaagga
     3721 tcatcaatgc tccagcaatc tttggctgaa ataaaatcag aaaagttata cctactgtaa
     3781 tcaggaatgg tttttttaga tcttttgggc tactatttcc gtcacgaaaa aaccagctta
     3841 aataaaaaat caaaaaaaga tttgccagtt cagaaggctg gaattgaatg ccaaacagag
     3901 agatccaacg ttgtgcgccg ttgacagtga caccgaatat ccctattctt actaatacta
     3961 aaaagaaaat cgataaggct aaaccgtatc ctgctatttt agggtgaagt aaatagtgta
     4021 gtttgactga acgagctaag aagatcacgc tccaactcaa aaatatgaaa attagttgac
     4081 gcaataatag actttttgta ttttcatcgg cttgcaataa acgataagaa ctggcactgt
     4141 atacttctaa aagaccgatc atggacaaag tcaaataagg acccaaaatc caccaatcga
     4201 tcttttttca ccgcatctcc tcctcacaca gcaatccact tgtcaggtga ggagcgagtt
     4261 tgtctcatca aataagtcag tcacaaaaat ataaagaaaa ggtcagaaac cttttcgaac
     4321 aagaattctg acctagcatc atctcatcgt ccgagtttta gcactataca acgtaaagaa
     4381 aacattctta gctattggaa aaagccttaa tccaacagtt tctgttttac ccattggcga
     4441 ctttcgacgt ttctgggcaa cagcctatgt ttgtatagga gcctcaccta acaaggttat
     4501 tactatattc tatttacgag ttcatgatag acttttccta atgattaggc aatggaattt
     4561 gaaatgaaat agtgaaattt aacaatcttt aatatttttt agaaaaaaat aacaaataag
     4621 ttgaaagaat cccgttgcaa attctttcag ttgcaccagg attctttgat aattttttag
     4681 ttttatgtgt ttttttatat gttttttcac ttttcggaat aaatatttat ttgttaaagt
     4741 aattaaacaa tccgctaatc gtttgatctg gcatttctat ttccttacca tctaaagtaa
     4801 cagtagtaac gacaaaatgg tcttgcaatg gcggaacttc taagtactgc ccttgcagaa
     4861 aatcattcac tgtttcatat tcttttttca tttcttttga atctgtcgat ggtttatagc
     4921 ttatttttaa cataactagt gctccttttt attcgggacg tgcaatcgtg aaaatttctc
     4981 ctattgctgc ataatcattt cctgccaaac ggcttacagc attcaatttt cttggatcga
     5041 tccttcctgt cttatcatca tagactgttt catttaaatg atatttctgt acttctagca
     5101 aaaataaatc agctgttact ttatttttat tccgtatctc gacatgttca tataaggatg
     5161 tttcaaaacg aacttttgct tctgtcaatg ctggtactga aatgattact gaatcagtta
     5221 aagtcaagtc agtcaggtac agttcgcttt cctcattagg cagattggct gctgttttat
     5281 ttacttcttc tacgttgtcc tcatcaagga tatggataac agcttctctt gtctcaagaa
     5341 tattctttgc tgtgtctttt actgcatctt gttttctctg gatcgccaag gataagatgg
     5401 gtcgattcga actgacgata ttgaagtaac tgaagggtgc gatattcact atatcttttg
     5461 ctgatttagt cgttactact gctactggtc tagggatgac tgaaccaatc agcaacttgt
     5521 aattttcacg ttctgtcaat tctgaaggac tccgttcaaa catggtttcg ccttctttct
     5581 ataaaattcg ttaaaaagca gatcttgaag acaaaatgaa aagtaatgct tttttcatct
     5641 tcttagaatc tgctttaaac tatctatcaa tttttctttt ttgtcaagaa tctgtgccgt
     5701 gcgtgttttt tagttatttg ctttacttgc ggcttcgaca tatcttttag tgatcaattg
     5761 cggacgggtg ataatagatc caactacgac tgaaaaagca cctaattcat atgctttctc
     5821 tacttgttca ggagagtcag tatgtccttc cataattact gggatatcga cttgttctat
     5881 gatttgattc aataaatcaa aatcaggtgt ctgcgtcttt cgtgaatatt ctgtatagcc
     5941 tgctaaagtc gtagacaaaa gatcaaaacc atcttcttgc gcttgaagcg cttcctcaaa
     6001 agtcgaaata tctgccatag ctaaccgacc gtgttcgtga atcatttgga caagttcagc
     6061 agttgttttt tctgtcggac gtttacgaga agttgcatcc agtgcaatga tttctgcttg
     6121 cgtctccaaa agttcttcca cttctttttc tgtcgcagta ataaacactt ctgacccatc
     6181 gtaatctctt ttgataatcc ctattaccgg taaatcaacg attcgcttga tttcattgat
     6241 atcttctgtc gtatttgctc gaataccaac tgctccgcct tcttttgcag ccaaagccat
     6301 tctccccata ataaaagaac tgtgtagcgg ctcgttttcc aaagcttgac aagaaacgac
     6361 gactccgcct ttgatttgtt ccagtgtatc cacaatatct cctccagtaa tgttatataa
     6421 aaatacagct tcatataaaa atgtaaccct tttctattca ggagacaata atgagctatt
     6481 tctttcagca gacctttctt actaaaagaa aattacttca aattcttcgt atacgaccag
     6541 cttctcttct gtaaattcta tgttcaacgc ttctttataa gcatgtttaa aaaactcagc
     6601 atggactttc cgccaataag ctaatgtccg atccccttct ccttctttcc aagcatgctc
     6661 tttcgtgact tcactaaaag gcaagatggt cacatttata tttttgatga tacaaactgg
     6721 ttcatttttt ccattcagga ccacgtctaa ttgaccaact tttggtaaag gctcattctc
     6781 ttgtttatac agatcgtaac cacttgatgt accagtcttt atctttttta caactagttc
     6841 gctcaactcg tctgccattt tttctgtatc cccaaatgaa aagaaaccta atttttctga
     6901 ttgataacta ggatattttt cttggaactt tttgacatat tcttctactt gctgatacat
     6961 aacatccacc cctttgcttt ttataatgaa aaaaccatcc agatactagt ataaacaatt
     7021 atctggatgg tgagcataat gaattattga atattcaaga cacgcagact atcttcaacg
     7081 atttgttttg gtgttaaatg ataacgttca taaagctgcc ctacaggaac gctatccgtg
     7141 aattctttct ctgcaccaaa atttaatacg ttcattgaag aatcaccata ataacgactg
     7201 atattttcgc caaatcctcc tgacaaacta ccatcttcta atgtgatgac taattcgtgt
     7261 gtttctttta atttatctaa tgtttctacg tcattttctg tgatcgttcc tggattgatc
     7321 aacgttgctt cgattccttt ttttctaaaa gcttttactg tttcttttcc taattcaaga
     7381 aaaccgccta aagctaaaat tgctactttt cctccattat ttactgtttg ataggctgga
     7441 atactataat catttgcgat agctgtacca tgttctacac catgtgtagg aattcggatt
     7501 gctacaggct gcgctgtttg ttcgaacgcc caatctagca tagagatcat atcctctttt
     7561 gttgctggag caagatattt gatgttcggg atacttgata agtaaggaat gtcaaatgtt
     7621 ccttggtgtg ttcggtcact agctgagatc gttccgccac cgactaaaat aatcactgga
     7681 ttctcgttga tcgctaaatc atgtgataat tgatcatatg cccgttgcaa gaaagtactt
     7741 gattggaaga cgactggacg tactccctga gaagccaaag cagaagcgaa agtgatagag
     7801 tgctgttcag cgatccccac gtcaaaataa tgatctggat gtttttcttg gaatgttttc
     7861 agtccaaagg cgcctggtat cgctgcattg actgcggcaa taggagctgt acttgtttgc
     7921 attttcttat ccaaataatc catgatgatc atattaaatg tttctccaga agttacttgc
     7981 tttgcctccc cggtggacag atcaaatggt acatgccaat gatatttcat tttattatcg
     8041 attgccggct gataaccgcg acctttttcc gtatggacgt gaagtacaat cggatgatca
     8101 atatctttta cttcttcaaa taaacggatc accgtttcca gatcgtttcc attatccacg
     8161 taacgataat caaatcccat tgctttaaat aggttattgc tgcttttacc gttggtttga
     8221 cgaagctcag ctaagttttt atataaactg ccatgattct catcaataga catctcatta
     8281 tcgtttacta atacgattag attggattgt aaaacaccac cattgttcaa tccttcaaaa
     8341 gctaagccac cacttaggga gccgtcaccg ataaatgcta ccacatttcc tgattctttc
     8401 ttcagatctc tcgctttagc taaacctgtt gctaagctga tagatgtaga tgtgtgcccc
     8461 actgtaaaat agtcatgttt actttcatgt tggttggaat atccgctcac ttcacggtaa
     8521 tgtccttttt caaaaccatt ttttcgacca gttaaaagct tatgaccata agactgatgg
     8581 gaaacatccc aaacgatttt atcctttggt gaatcaaata cataatgaaa cgcaatcgtt
     8641 aattctacta cacctagatt cggaccaacg tgcccaccta catcgctgtc cttttgcaag
     8701 attttttggc gcatctcttc tgccaactgt ttcatttcct caacagataa ttttttgaca
     8761 tctgcggggc tattgattgc atccaataca tgtaatactg tactcataat acattctctc
     8821 ctccgtacta tcttctttta atgaaaatag atttgttaca ctttatctat tctactactt
     8881 agagttgact ctaagtcaat agcattttaa aaaaatttca gagaaagctt gttatataag
     8941 tagatcttcg cacgaatcag gactcaaata aaaaccttct acagacaatt aaaaagcttt
     9001 gtagaaggtc tcctaattaa ggtctcatca agaaatatgt ttttttaata ggtgaagttc
     9061 ctgagcaata tgatacaaat ggatgtttgc accttttaat gagattggat agatctttgt
     9121 cttttcagtt tctaatccat tttgatagct agcagcatcg cacaaaacca ctcttttttt
     9181 cgcacataga cattcatcag gcaattcttc taatgaacga aataaaatag gatttatttc
     9241 agcgatgcct tcttccgcat acaaatgaaa ttgggttcga taaaaaaatt gtttgactga
     9301 actttctgaa acaacaaaaa tttctatttc ctgtctcccc gcttgttcac tgttgatcaa
     9361 agaaagactt ttgacgaatt ttcgaatgaa attttcattc catcttagtt ggatttgatt
     9421 tttctttttc cactcttcca atatttctac aatttttccg tacagaagtt gttctttttc
     9481 tgatagaaga tagatccttt ccggtaaaaa tacttgtcca tctgcccaca acgtacggac
     9541 aaattgtaaa aaggattgct tgaaaataaa attttctgca tcgtcgattt cagcgatact
     9601 aataatctgt aaaatcaatt cattcagtga taggtcattg agaacaaaat ttttataaac
     9661 aacttcgaaa tcttttctta acagctccat gttctcattg gtataatttc tactgttgaa
     9721 caaagaaaaa atataataga catcttcttc ttgtaagtct agaattcctt tttgctcttc
     9781 caatccttga cagactaatt gaaataacgg cttcgtttct gcgcttttct tatcttcttc
     9841 tttcagtcca aggggaaatg cctttcttcc gattgcgatc tctattcctc gtatgataaa
     9901 ggacttttca tcttccgaat atcttctttt aaaaaaatga gtctctacat aatcaatcaa
     9961 ctcgttacaa gcttcttcta catacggatt tttattttca tagccttccc aattgacata
    10021 acgtactaaa gctagcaaga tgttccgcga gtcaaattct gggataatga tttctttatt
    10081 tttcaaataa tcattttctt taaaaaaatc caatacgatt tttttgattg tatatacttt
    10141 gctcaaagaa ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata
    10201 ataacttaaa aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag
    10261 agcaatacca tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat
    10321 acaatattct agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac
    10381 ccttagagaa atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca
    10441 cagatacact tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg
    10501 cctccagttt ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt
    10561 tttatttaga aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac
    10621 aagctcacaa tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc
    10681 ctctttcaag aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa
    10741 caattggata ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat
    10801 gccgtaacga tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac
    10861 accaacatgg acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga
    10921 tttgacgatt gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc
    10981 tttatttgcc agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca
    11041 ttgtctgaaa taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt
    11101 catatagtaa gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc
    11161 ttcattcaat gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc
    11221 aaatttttca tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt
    11281 aacaaaagga ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa
    11341 ggcaaaaccg gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt
    11401 gattgctaat aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc
    11461 gtctcctccc tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt
    11521 gggatccact tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg
    11581 gaaatttttt ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca
    11641 ttttttattt ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa
    11701 ctaagacgat aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg
    11761 cgatactagc aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta
    11821 aacaagcaac gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac
    11881 tgtcatcttt aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt
    11941 agattacaac agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta
    12001 atgtaaagac ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag
    12061 gaacaccgtg tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta
    12121 cgtgaagtgc ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac
    12181 aaacaagaat cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata
    12241 ggaattctaa cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag
    12301 tagcgacggt caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca
    12361 ttgctaatgg ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt
    12421 ctacgcccat atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa
    12481 aatcaggaat caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat
    12541 aatggatccc taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga
    12601 tccgagcaat caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga
    12661 aaataatcaa taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg
    12721 ttgcatagga taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct
    12781 caaaccattg gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc
    12841 aagtatagac accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg
    12901 ccacagggat aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg
    12961 aggtcgcgaa tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa
    13021 agccaaagaa cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc
    13081 ctcttttcta tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc
    13141 cttcttcttg ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa
    13201 tcactgttgt cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc
    13261 ctaagaagta ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta
    13321 tcagaatcgt acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat
    13381 cccattgttt ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc
    13441 caatcagcat ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc
    13501 cagcaacgga tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa
    13561 tgatcagcca agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga
    13621 ctgcttgttc atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa
    13681 gcagtcctgt accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag
    13741 gaatctttct ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt
    13801 ggatgactgc tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc
    13861 atatccagtg gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat
    13921 agccttttaa attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga
    13981 taaacatcag ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt
    14041 tggggaagct tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc
    14101 cgatatcccg tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc
    14161 caacggagag aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc
    14221 cgtaaaaaaa agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga
    14281 atgctgtatc tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat
    14341 tacgagtata aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc
    14401 atttataaaa gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca
    14461 cacaaataaa gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct
    14521 ttttttattt ttaaagatat tttttcataa ggaataaaga atacttcata taaaatatat
    14581 aaaaaagaac cgcagcgagt gtcgctacgg ttctcaaatt tgttcttcat ttcaacataa
    14641 gtttttcttc aatttgctta taatttacgt gcaaagaatg aaacgactaa tactaaaatg
    14701 attgcaccga tcaatgatgg aatgatagcc attcctgcaa ttgttggtcc ccaagttcct
    14761 agtaaagctt gtccaattgc tgaacctact aaaccagcaa tgatgtttgc gatgatcccc
    14821 attgatgatc ctttattcgt gattgctcct gcaattgctc caatgatacc gccgacgatt
    14881 aatgcccaaa taaatcccat tttaaattcc tcctaactga cttcttactt cttattgtac
    14941 acgtggttcg ttattttctt tgactttttg tgcacctttg cctacagcag attttgcttt
    15001 atctgtttgg cgtgaagcaa attgtcctgt ggattttgct gcttctgtta ctttgtcttg
    15061 tactgtttct tgatcttttt ggtattcttc ttgtgttttg atgtctgcaa cattcacatt
    15121 cacttcgata acatctaaat gtgtcatatt gtttacttca gtactgatca attctttgat
    15181 ttggttgtaa atatcttcga tgtctttacc gtattctacg atcacatcta gatccacagc
    15241 tacttgtttc ttacctactt ctgtattgat acctgctgtt acgttatctg tgttgactaa
    15301 tttttcagcc atatttgaga aaaatccgcc atctaatgtc aatagaccat ctacatcttt
    15361 caatgcataa ccgatgattt tttggatgac tttgtcttca taagtcaatt caccattcat
    15421 catgcggttt tctccttttt catttgccaa aactgatcca tttgttgtat tcatgttttt
    15481 gttttccatc tttgtttcct cctatgattt aaaaaattga tcaactattc ctgactgttt
    15541 caaataaaaa ccgattcctg cgccaattgc tgtcatgatt atgatcaaca atgtcttgaa
    15601 aaatccaatc gttaaccata aaacagctag cagtagtcca agacccccaa agataatagg
    15661 gagcttgtat gctgaaaata gttctttcat tatttggccc tcctattcca ctcgagagtg
    15721 cttgtagtct cttgtatctt cttgttcttc ataaccgctg tatgtgactg ccactttgac
    15781 cgtttctttt gttccgagta ctttagttac ttcttcttcg atatcttgca tcaatacacc
    15841 tgttttccct actaatgaag aagttcgttt caagtctccg ttgacatgga tatggattcg
    15901 atttttcgtt gaacgtatcc gtactttagg tgaatgaatg aactcttctt cgtgtaaatg
    15961 ggaacgtacc aacccttcga tcgctttttt gtcgattgtt agtttgccat cttcccgttt
    16021 cataacgaaa gttcctcttg actttggata gaaaagaacg actagcaaga tgataatagc
    16081 aagaacggca aatactacag ctgcccagaa aatatattgt tgcatgtagt aattcgttaa
    16141 agtaaagaag cgaaatcgtt ccaattgttc aggcataata tacggcgcat tgatcaacgc
    16201 aatcgctaga aatactgaaa gtagcagtaa actcatgata acaccgatta tttttaaccc
    16261 cttattcatt ttccacctcc tataaactta tttttaccct gtaaataact ctaccgacaa
    16321 tcacagtata gatagcgaac agttagcata gcatcagtaa ataaagcgct accaaacatt
    16381 gctggatcat aacggcgttt tattgccagt ctgatactga aataacaatt gcttacttat
    16441 tctctattta cttttgattg ctggctatac ttaaaggata ttattcaaga ttaaaaattg
    16501 caaagattat gctcagttat tattacaaga ttctatttat t
//
LOCUS       DAZKCZ010000055        16538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000055 DAZKCZ010000000
VERSION     DAZKCZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.055"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..347
                     /locus_tag="ACG8KN_001628"
     CDS             <1..347
                     /locus_tag="ACG8KN_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9188688.1"
                     /translation="LEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKIFQSMS
                     RKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQS
                     LVQFRKTTVTVV"
     gene            536..1204
                     /locus_tag="ACG8KN_001629"
     CDS             536..1204
                     /locus_tag="ACG8KN_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9188689.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1404..1724)
                     /locus_tag="ACG8KN_001630"
     CDS             complement(1404..1724)
                     /locus_tag="ACG8KN_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188690.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1761..3143)
                     /locus_tag="ACG8KN_001631"
     CDS             complement(1761..3143)
                     /locus_tag="ACG8KN_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9188691.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3299..4486)
                     /locus_tag="ACG8KN_001632"
     CDS             complement(3299..4486)
                     /locus_tag="ACG8KN_001632"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9188692.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4493..5524)
                     /locus_tag="ACG8KN_001633"
     CDS             complement(4493..5524)
                     /locus_tag="ACG8KN_001633"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9188693.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5540..6994)
                     /locus_tag="ACG8KN_001634"
     CDS             complement(5540..6994)
                     /locus_tag="ACG8KN_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFP9188694.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            7169..8074
                     /locus_tag="ACG8KN_001635"
     CDS             7169..8074
                     /locus_tag="ACG8KN_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFP9188695.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8165..8842
                     /locus_tag="ACG8KN_001636"
     CDS             8165..8842
                     /locus_tag="ACG8KN_001636"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9188696.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8839..9009
                     /locus_tag="ACG8KN_001637"
     CDS             8839..9009
                     /locus_tag="ACG8KN_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188697.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9123..10019
                     /locus_tag="ACG8KN_001638"
     CDS             9123..10019
                     /locus_tag="ACG8KN_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9188698.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10074..11444)
                     /locus_tag="ACG8KN_001639"
     CDS             complement(10074..11444)
                     /locus_tag="ACG8KN_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9188699.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11682..12428
                     /locus_tag="ACG8KN_001640"
     CDS             11682..12428
                     /locus_tag="ACG8KN_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9188700.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12425..13264
                     /locus_tag="ACG8KN_001641"
     CDS             12425..13264
                     /locus_tag="ACG8KN_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9188701.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13265..14215
                     /locus_tag="ACG8KN_001642"
     CDS             13265..14215
                     /locus_tag="ACG8KN_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9188702.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14356..15108
                     /locus_tag="ACG8KN_001643"
     CDS             14356..15108
                     /locus_tag="ACG8KN_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188703.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15127..16278
                     /gene="queG"
                     /locus_tag="ACG8KN_001644"
     CDS             15127..16278
                     /gene="queG"
                     /locus_tag="ACG8KN_001644"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9188704.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5382 a         3069 c         3311 g         4776 t
ORIGIN      
        1 gtttggagga agctatccag accactaacg attgtcccta tcgtcgtacg tttcactcag
       61 atcaaggttg ggcgtatcaa atgaaagctt atagaaacaa attgaaggag tacaaaatct
      121 ttcaaagcat gtctcggaaa gggaactgct tagataattc acttatggag aatttttttg
      181 gcttattaaa acaagagatt tttcacggga aagtttataa ctgtttcgag gaattaaaat
      241 cagccatcga tagctatatc tattactaca ataatgaacg gataaaacag aaattaaact
      301 ggcaaagtct cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta
      361 gagcataaaa aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac
      421 tacagtgtca cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc
      481 gtgttcgtga gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat
      541 tttttcaatt acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt
      601 gcttgggaag aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt
      661 aggtgggatc ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt
      721 tgccattgcg ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga
      781 aaaggtcaat cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa
      841 catgacagag ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca
      901 gcaagaatgc ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat
      961 gagtgtattg aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg
     1021 gcaaattcag cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa
     1081 cttgaagaaa gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga
     1141 aaaagggctc tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg
     1201 atagatagac agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt
     1261 tttcacaacc tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag
     1321 ccaaaattcc acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag
     1381 ttgatcactt ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca
     1441 atataccgta cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag
     1501 gcttcaccaa tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa
     1561 ttgagctgaa tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt
     1621 tcttgcaatg tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt
     1681 tgataccgtt gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc
     1741 gatctcgata ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct
     1801 tctaatccac tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt
     1861 cctccttgca agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc
     1921 gtatcgacaa caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg
     1981 ttaagctgta aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata
     2041 tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta
     2101 gcatttgtcg cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga
     2161 tagcctaaag agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc
     2221 aagcgagtgc cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt
     2281 gtataatctc tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct
     2341 tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc
     2401 cctagcttgc ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata
     2461 cccatttgtt tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct
     2521 gagattttcc ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt
     2581 ccgtcttttg agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct
     2641 atagtcgtgt agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg
     2701 tacgcatttg ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg
     2761 acggaataga catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct
     2821 ctgttctttt ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag
     2881 gggaagggaa gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca
     2941 ggactggaag ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac
     3001 atgactacta atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg
     3061 taaaaagatt tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat
     3121 accggatttt gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc
     3181 tctttccgtg tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga
     3241 aattgctgag aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc
     3301 aaacatgaac agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa
     3361 attccggagc atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag
     3421 gtatattttc ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc
     3481 gtgcacctaa gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc
     3541 cagttacttc ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg
     3601 cttctaagtt cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa
     3661 cggcatggcg agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa
     3721 attcttcttc atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca
     3781 aggcttcttc gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct
     3841 tattcgtatt catgatgacg attgcataac catctagttt cactggaacc atttcgtatt
     3901 tcagcgtatt tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact
     3961 gatccatgat acctgaattg acaccgatga attcattttc tactttttta cctgtttgga
     4021 ctagttccaa gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata
     4081 attctaatga agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata
     4141 actcaaaccc atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta
     4201 cgtaatttgc ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga
     4261 caccgatatc gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg
     4321 ctacaccttg cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg
     4381 tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc
     4441 cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct
     4501 ttcttgtttt gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa
     4561 tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc
     4621 caccgtgatg tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag
     4681 tgaaaataac cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc
     4741 tagtcaagcg agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga
     4801 ttccgtcaac aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag
     4861 gtatttgaaa atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat
     4921 ctgttcgcaa aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact
     4981 gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt
     5041 ggtcactggt gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga
     5101 tttccacatc cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa
     5161 tgacagaagc ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc
     5221 caggagcacc accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt
     5281 ttccattgag agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac
     5341 cgatatacgg atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc
     5401 gatcatttga ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct
     5461 tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct
     5521 tcattttcaa gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca
     5581 ttgtatcagc tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct
     5641 ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag
     5701 catagaatgc ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag
     5761 cattaccagt catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac
     5821 cagcgatttt atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat
     5881 ttttccattg accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca
     5941 attgttgagg gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg
     6001 cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc
     6061 gacgagagat gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg
     6121 cagcaactag accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt
     6181 agaataatac ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa
     6241 gccacatcaa ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt
     6301 cattttgctg acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag
     6361 taccgcctga aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa
     6421 tatgtttatg agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag
     6481 taccatttgc acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct
     6541 ttgtttccgt actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa
     6601 aactatccaa taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag
     6661 tagctaagcc aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc
     6721 cgataactaa ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac
     6781 caatcaaagg atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg
     6841 tagcaatcat ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga
     6901 ataccataaa ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag
     6961 cgtaggacac acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata
     7021 tataatacta agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta
     7081 acaagaaatc gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat
     7141 tatactaaag gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca
     7201 tcaacaagta gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt
     7261 tcattctttt ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa
     7321 aggatcttat catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat
     7381 tcgtccagga gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg
     7441 gctttcattt ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga
     7501 ccaatacacg atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat
     7561 gaaagatacg gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt
     7621 tttatcagtc cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc
     7681 ctctttagct gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt
     7741 tgaggaaatc gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa
     7801 tcatatgggt accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt
     7861 tcttctttct ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag
     7921 tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg
     7981 taaacgtatg aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt
     8041 ggaaaaacaa accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc
     8101 ggcaaaagaa acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa
     8161 agggatgcgt acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca
     8221 atttttcaaa gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta
     8281 tccagacgga aaaatagata tagagactga actacagctc ttggcccagc atgatcggat
     8341 cattttccag tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga
     8401 taatgtcttg gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac
     8461 aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga
     8521 atttttgagg ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt
     8581 cgttttagca caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta
     8641 tcagcagtat ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat
     8701 tcaaagagta caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat
     8761 cgtagaagca ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga
     8821 aatgaaagga acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc
     8881 gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag
     8941 atagacgaac gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc
     9001 aactggtgaa attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg
     9061 ctacaataag aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa
     9121 aaatgtatga tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa
     9181 aacaggcagc tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca
     9241 tagcaggagg agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta
     9301 cgaccataac ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa
     9361 ctaaaacaaa agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac
     9421 ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg
     9481 aagaatacca gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg
     9541 ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg
     9601 cagcgtatca ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat
     9661 tatcggctaa ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg
     9721 cctctcttca agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag
     9781 cagagtcggt agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct
     9841 ctgaaactaa taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag
     9901 aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga
     9961 atgggatgag catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat
    10021 aaaagcaata aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt
    10081 ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca
    10141 ataatcaatg agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa
    10201 aaagcaagtg tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc
    10261 attttatctg cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga
    10321 accataccag gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg
    10381 atgaacattg gtagaagata atttaagatc agaccaatta agattccacc tgagattgct
    10441 acgacaggat aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt
    10501 ccatttatgg caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct
    10561 gataatgccg ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact
    10621 ttagaaaaag tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata
    10681 ctcacaatga taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg
    10741 acagcttcaa tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt
    10801 tcgattcctt gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct
    10861 ccagtgaaga atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta
    10921 ccaaatccaa ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact
    10981 ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc
    11041 agcataagaa acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa
    11101 taattcatat aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa
    11161 atattactcc aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt
    11221 gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa
    11281 tacaaaaaga atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg
    11341 gttgaggagg cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg
    11401 atatgacggg cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg
    11461 ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt
    11521 tagccttatt gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat
    11581 tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat
    11641 ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg
    11701 taattcaaga cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga
    11761 cgataaatcc acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat
    11821 ttatgaaatc tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac
    11881 agtcggtaaa ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc
    11941 tttcctttcc aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa
    12001 ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag
    12061 ggatggaaga atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag
    12121 tttttattgc aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg
    12181 tagggattga tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt
    12241 caggaaaaac gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg
    12301 aggttattct tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat
    12361 cgaagaacct tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga
    12421 aaaaatgatc caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt
    12481 ggtcacttcc atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg
    12541 agggatgtct ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc
    12601 ttatatgttt ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc
    12661 agtgattggt ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt
    12721 ctttagctcc ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga
    12781 tttgtatcat attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac
    12841 agcactcgtt agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat
    12901 cacatcattc gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta
    12961 tcttttgatg ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat
    13021 cttggtgatt gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt
    13081 actgaaaatg atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt
    13141 tttcagttat agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt
    13201 ttttatcctt gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag
    13261 atagatgaaa acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc
    13321 cggttgcgga aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt
    13381 cgtggctacc aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga
    13441 tatccacagt atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga
    13501 catcaaaaag gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg
    13561 taacagctgg ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt
    13621 tgcagttagc aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc
    13681 agatccccat gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg
    13741 tatattctct gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata
    13801 tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga
    13861 gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga
    13921 tttgccagca gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat
    13981 gaaagcgatc attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag
    14041 tgtagactca agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa
    14101 actgttcact gattccatag caaaagaagg agaacaagga gattcttact atcagatgat
    14161 gaaatggaat attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt
    14221 gcaaggtcta gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta
    14281 tgctgttttt cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg
    14341 aaggaaggtg tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc
    14401 tttcgattga tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa
    14461 gcagaattag ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag
    14521 ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc
    14581 ggttattttg tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac
    14641 gacgaaataa ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta
    14701 atcaaaaaga tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg
    14761 aatatgtttt ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc
    14821 acgtacacac agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag
    14881 ttttcgccat gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg
    14941 aattttttta atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta
    15001 tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt
    15061 gtccagctga aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg
    15121 aagaccatga cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa
    15181 atcgggttca ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa
    15241 gcagctggcc atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa
    15301 atgacatttg atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc
    15361 catgaagaag tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc
    15421 gattaccatg atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga
    15481 gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa
    15541 ctgatcgatg tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg
    15601 ttgatcactg aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta
    15661 tttgagcctg ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct
    15721 tgtccgacaa aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat
    15781 caaactcaga caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt
    15841 tatggatgtg atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt
    15901 catgaagaga tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg
    15961 atgtctaata aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag
    16021 aaacctttgc agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg
    16081 cctgagattt cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg
    16141 gctattggac gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata
    16201 ttagctaaag aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg
    16261 cggagaaagt taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt
    16321 tgtaacaatt actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat
    16381 caaaaatttt tttgcttttt tttatggatt attgcagtga atgttacaga atctgcgttt
    16441 ttcattcaaa aataaaaaaa catagaaaaa cgattacttt gcttatacaa caaggctttt
    16501 atggttttgt atcaaaaaaa tatttgttta aatgttac
//
LOCUS       DAZKCZ010000056        15900 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000056 DAZKCZ010000000
VERSION     DAZKCZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.056"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACG8KN_001645"
     CDS             complement(52..522)
                     /locus_tag="ACG8KN_001645"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFP9188705.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACG8KN_001646"
     CDS             complement(681..1139)
                     /locus_tag="ACG8KN_001646"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9188706.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACG8KN_001647"
     CDS             complement(1310..2455)
                     /locus_tag="ACG8KN_001647"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP9188707.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACG8KN_001648"
     CDS             complement(2468..3385)
                     /locus_tag="ACG8KN_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFP9188708.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACG8KN_001649"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACG8KN_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9188709.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="ACG8KN_001650"
     CDS             4477..4647
                     /locus_tag="ACG8KN_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188710.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="ACG8KN_001651"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="ACG8KN_001651"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9188711.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="ACG8KN_001652"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="ACG8KN_001652"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9188712.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /gene="dapA"
                     /locus_tag="ACG8KN_001653"
     CDS             6911..7792
                     /gene="dapA"
                     /locus_tag="ACG8KN_001653"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9188713.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /gene="dapB"
                     /locus_tag="ACG8KN_001654"
     CDS             7789..8505
                     /gene="dapB"
                     /locus_tag="ACG8KN_001654"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9188714.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="ACG8KN_001655"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="ACG8KN_001655"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFP9188715.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="ACG8KN_001656"
     CDS             9222..10223
                     /locus_tag="ACG8KN_001656"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9188716.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="ACG8KN_001657"
     CDS             10220..11581
                     /locus_tag="ACG8KN_001657"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFP9188717.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="ACG8KN_001658"
     CDS             11541..12692
                     /locus_tag="ACG8KN_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9188718.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="ACG8KN_001659"
     CDS             12667..13836
                     /locus_tag="ACG8KN_001659"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9188719.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="ACG8KN_001660"
     CDS             14011..15390
                     /locus_tag="ACG8KN_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9188720.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="ACG8KN_001661"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="ACG8KN_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5202 a         2802 c         3384 g         4512 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga ttgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DAZKCZ010000057        15564 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000057 DAZKCZ010000000
VERSION     DAZKCZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.057"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..486)
                     /locus_tag="ACG8KN_001662"
     CDS             complement(<1..486)
                     /locus_tag="ACG8KN_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9188721.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLRNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LK"
     gene            718..993
                     /locus_tag="ACG8KN_001663"
     CDS             718..993
                     /locus_tag="ACG8KN_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9188722.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMEQYKG"
     gene            1091..3088
                     /locus_tag="ACG8KN_001664"
     CDS             1091..3088
                     /locus_tag="ACG8KN_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188723.1"
                     /translation="METPMINIPINVDKERLLEKPVIEDEKKRIRKRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNKSEEFLETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKALSINLVHNSAFAIQKSIRYSENLEKFKIVFLLKEAITDYSKMVAVYEYLKKI
                     IPGCDDNVNASNRMFFGGYKSDAVIMINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMDKLLKSSKNL
                     RCLFHDDHNPSASIFTSKTGHSIFYCHSSNCSISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMEDKLFRTIRNYLEEMRHVYDILLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGVVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(3164..3499)
                     /locus_tag="ACG8KN_001665"
     CDS             complement(3164..3499)
                     /locus_tag="ACG8KN_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9188724.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            3627..3842
                     /locus_tag="ACG8KN_001666"
     CDS             3627..3842
                     /locus_tag="ACG8KN_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9188725.1"
                     /translation="MKKVSNLSRYRKEKGFSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            3879..4241
                     /locus_tag="ACG8KN_001667"
     CDS             3879..4241
                     /locus_tag="ACG8KN_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFP9188726.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            4287..4613
                     /locus_tag="ACG8KN_001668"
     CDS             4287..4613
                     /locus_tag="ACG8KN_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188727.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYSLNRTCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYT
                     EVLMLN"
     gene            5100..5237
                     /locus_tag="ACG8KN_001669"
     CDS             5100..5237
                     /locus_tag="ACG8KN_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188728.1"
                     /translation="MAHNERFKQEIIQELEKGVQHQKLISKTAQKFDISLAKEVKLHE
                     V"
     gene            5302..6084
                     /locus_tag="ACG8KN_001670"
     CDS             5302..6084
                     /locus_tag="ACG8KN_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188729.1"
                     /translation="MKNSSNSAEVNVSTSTSEAVQCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDGVKYSEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKKVSGENNETEAVTEQTMDERIDSSESNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKNVPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(6578..7855)
                     /gene="dcm"
                     /locus_tag="ACG8KN_001671"
     CDS             complement(6578..7855)
                     /gene="dcm"
                     /locus_tag="ACG8KN_001671"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFP9188730.1"
                     /translation="MSETLNVLELFAGVGGFRVGLEHSNADLFKTKWANQWEPSRKSQ
                     DAFEVYDYRFPNSENINRNIEEISNEEFEQMDADIIVGGFPCQDYSVARSKKHEMGIE
                     GKKGVLFWQIIRATNHIKPKYLILENVDRLLKSPSKQRGRDFAIMLASFNDLGYSVEW
                     RVINAADYGRRQRRRRVFFFVYKNDTGFANKIDARFEQKILGEEEHRYDEYIFSEGLF
                     ARQFPIIKTPYKGRQASYKLSNDIVEISDNFTGKVYNTGVMRHGRYFTIDTEPYDKEK
                     PTPLREIIQPEDEVAEKYYLTDESKIKKFEYLRGPKKIERVTAEGHTYTFSEGGMSPY
                     DDLDMPGRTMLTSEGSVNRSTHLLKIDGRYRLLTPIEAERLQDFPDDWTKYKKLSSGE
                     IVEVSDRMRLFFMGNALVTKIVERIGDELQQLL"
     gene            8078..9556
                     /locus_tag="ACG8KN_001672"
     CDS             8078..9556
                     /locus_tag="ACG8KN_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sau3AI family type II restriction endonuclease"
                     /protein_id="HFP9188731.1"
                     /translation="MSEYLTKEQVHNRAKEAVGKSMIELNNGIPLLNSKNKNSIGDIF
                     ESWFGKVKDSESRPDLEEAGVELKATPIKKLKNGQYSAKERLVLNIINYNEVAEETFE
                     ESHFLYKNNIMEIAFYEYLKDTPKDQWTIREAILYEMYKNPVDFEIIRQDWELIKKYI
                     LDGKAHELSESLTNYLAPCTKGTNAVKSLRTQPYSEQKAKQRAYSLKSGYMTSILRKY
                     VLGDEKIDSIVKNTFQLEENNLEDLVLEHFIPYVGQTVQSLCEKFTIKTSYQANYRIV
                     SSILGLSGTVTKGEPFPRVEEFEKASILVKTIYFDKSGNNKESMSFPSFNFIDLSKQV
                     WEKEDGEPSSDWHNFLLDTRFLFVVFKEEDEGVVFKGAKFFSMPEEDIEGPVRKVWED
                     TVSKLREGVELEAIPDKSTKDGWKIKNNFIKKTDKMICHVRPHASQRDYSVDGRYADK
                     LPVPAKWINKPDNDNYSDQWMTKQCFWINNDYIKEQVQDLIS"
     gene            complement(9570..10001)
                     /locus_tag="ACG8KN_001673"
     CDS             complement(9570..10001)
                     /locus_tag="ACG8KN_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFP9188732.1"
                     /translation="MKHRQLSTTPEVSKRMANVRLKGGKAESLLAKELWHQGVRYRKN
                     YKKLPGSPDIAVTKYKLAIFVDGEFWHGHDWDNRKQKLKRNRDYWIEKIEENMRRDER
                     NNQSLIKLGWVPIHFWEKDILKNLNYCTSVILDYIAQKKEE"
     gene            complement(11573..12238)
                     /locus_tag="ACG8KN_001674"
     CDS             complement(11573..12238)
                     /locus_tag="ACG8KN_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9188733.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            12364..14400
                     /locus_tag="ACG8KN_001675"
     CDS             12364..14400
                     /locus_tag="ACG8KN_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFP9188734.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            14404..15042
                     /locus_tag="ACG8KN_001676"
     CDS             14404..15042
                     /locus_tag="ACG8KN_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188735.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            15070..15498
                     /locus_tag="ACG8KN_001677"
     CDS             15070..15498
                     /locus_tag="ACG8KN_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188736.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         5309 a         2321 c         2883 g         5051 t
ORIGIN      
        1 ttttaagata ttgaaaagac taattttttc atttttaact ttattacaag tcttgttagc
       61 aataaattct tgcatctctg gttctaattt ggaatatcta tctagccatg tatctaaatt
      121 agagtgtact ggtgggaact gttgacgaag atgctggaac aggtttttat ctggaaatgt
      181 tataccatct agtacaatgc tatctctata aagaataatt cccaaaatat attcttctac
      241 attgccacaa actaactcct ctatactcat atttctcaaa tcagcaattc gggataatcg
      301 ctctggtatt ggtaaatagg ttcccttttc ccattcgtag atagtttttt tgtcaatcaa
      361 ttgaccgttg ttaatcataa gaccaaatgc tggttgtgtt aattttttac tctttcttat
      421 agcttttatt tttgttccga gtatcttttt tcgttctaaa taatttattg gtggacgctt
      481 tatcatattg accacacctc ctaaaattag tataccttaa aaaaggggtg ttgtaaataa
      541 atttaacaaa aatattgaca cccctttttc gtcgaggtat tatgtgtatg tcgatgaact
      601 ggtaatcatt ttttgtttga ttaatcggtt aataacaaaa tacttttatt ttgagcaaaa
      661 aatacgaaga tgattcttag aaaatttgtt caagcaatca cgaaaggagg ggaaaatatg
      721 tctctacaaa tcattgatag cattgtttct atacaagttg aacaacaagt gaaagagata
      781 attacagtgc taaagcctat gctagaagac attatttctg gagtcactct agagtatatg
      841 gactatcagc aggcaagtga atatctaggt gtatcagtag gaacgattag aaaatatgtt
      901 tctcaatacg ggttacctgt tataaaaatt gatacggtca ttagattcaa aaaatctgat
      961 attgatagtt tcatggaaca gtataaaggt tgagtaaaca aataagacta aaacatatag
     1021 taataaagag tttgtgttta ttatctgtat gttttagtct ttaacagaat tttataagga
     1081 ggaaacacaa atggaaacac caatgattaa tattccaatt aatgtagata aagaaagact
     1141 attagagaaa ccagttatag aggatgaaaa aaagagaata cgaaaaagat gtttagataa
     1201 ctacagagaa gttacaagag atgagtttgt agagatacta aattcatcac aatcatttat
     1261 tccaagtaag acaaagaata aaggcaacaa aagtgaagag tttttagaaa ctagagttat
     1321 tattctggat gtcgataaca ctgttaaaga tgagaagggg aaggtagtag atttaagtaa
     1381 agatgatagt agatacttaa gcatagaaaa ggctttatct atcaatttag ttcataattc
     1441 tgcgtttgcg atacaaaaaa gtattcggta ctctgaaaat ctagaaaaat tcaagattgt
     1501 ctttctatta aaagaagcaa ttactgacta tagtaaaatg gttgctgttt acgaatactt
     1561 gaaaaaaatc atacccgggt gtgatgacaa tgtaaatgct tcgaatagaa tgttttttgg
     1621 aggatataag tcagatgcag tgataatgat taatgaagac aacatgttag atattactga
     1681 gcttccgatt gattttacca tgacatgttc taaaaattgt caaagtgatt cagggagaga
     1741 gtatgaatta gtcaatgaaa ctgattttgt taagttgata aaaaatgacg acaaagaaga
     1801 aatgaaacag tggtttaaag actgcttgtt tgattcctcc gacatgtctt tcaatgagat
     1861 ttatgaaaga ttgttaacca ttgatatgga taaattactt aagtcaagta aaaacttaag
     1921 atgtttgttt cacgacgacc ataatccctc tgctagtatt tttaccagta aaactgggca
     1981 ttccattttt tattgtcatt ccagtaattg tagtatcagt gaaaatttta ttggggttgt
     2041 gatgttaatt ctaaagaaag acagtcgggt agaaacattt aattggctat tagagaacct
     2101 agacctgtat cctacacatt ttcgaaaact gaaagaggaa tcaaaagtac tttttgacac
     2161 tcttgaatcg atggaagaca agcttttcag gacaataagg aattatctag aggaaatgag
     2221 acatgtctat gatatcttgc tttctgatgt taattttttt gatgatagta aggaaagtgt
     2281 gacttgtatt ttgtcggggg aacaattagc taaaaaaatg agttcttact atggtaaaac
     2341 ttatgacata gataaatgta ataagttact atcacttatg acttttatag gattgataaa
     2401 aaaattagat gatgaagatg ttcctttgaa gatgctcgaa tacctaaata ggttaaagga
     2461 agagaaagcg ttaggaaact ttaatcataa gagaagcaat gtctatcgat tagacgtttt
     2521 taatgcaact tctgtgattc aaaaattgaa tgaagatatc cttccacttc tggaaaaaat
     2581 tcattttaca tatcaatatt tttcttatga atgggttaag atatgtttta acgaaaaaga
     2641 ggctaaaaga gtatttcctc aaaattcccg atcagaactc agcaaggaaa aacaattgat
     2701 tttagatgaa gcacatgaag ttttacaggg cttatcgtgg caagggacat atctaatgac
     2761 agagaaagaa cttattaaca ctgtttgtga acgcctacgt aatgttgggg ataacagcgt
     2821 gaaaaatgct ttaataaaaa acagaggcta ctttgttaaa caaggattca ctcttgaacg
     2881 agcttctaag gatattaaga aactatacaa tgtgaaagca agtagtagtt ttttaatata
     2941 tttagcgaaa ggggcaggga tagatgaaac aataaaagaa ttgatattat caggagctgg
     3001 ggtagttcct ctgtatcaat ttacaagtgt aacaagagat gatggacgga gagtaaggaa
     3061 gagaaaagac ccaattgtat taaactagac aggttatcca tagctcttaa tcttatttca
     3121 tctcaataca cccaccgtta aagttcggtg ggttatttat ttttcaatct ttcaatagct
     3181 gattgcgttc attttcttgc ctttttaata gcttcatata gtcaattgtt cgaactgcat
     3241 cttcagtgct aagagaacga tagagagcca aaaactcttc ctcataatct acgacaacat
     3301 tgtctagaaa atggcttaca ggggttccta gggctctaga aagagctgtt aagctatcga
     3361 ggttaggttc tgttacagaa ttttcccatc ttgctactgc ctgttgtgta gagaagattt
     3421 ttttagctaa ttctttttgt gtccacccga gagcttttcg cttttctcta atgacctttg
     3481 catattgttt ttgtttcatt aatacacccc cttctcagta gagtacaaca agttgttgta
     3541 tttgtaatga tatttgaatt tttaatatgt tttatgagtt gaaatacatc atattgttgt
     3601 atataatgga acaggagggg agaattatga aaaaagtaag taatctcagc cgatacagaa
     3661 aagaaaaagg attttctcaa actgagttag ctaaaaaaat gaatgttaca cagcaatgtg
     3721 taagttcgtg gcaaacaggg agaacgattc ctaaacccta tcaaatgaaa atgctttctg
     3781 agatattaag tgtaccaata aatgagctgt tttctgatgt atttaataaa gtaaacagtt
     3841 aactaataaa caattgaatg tttttaggag gagaagacat gtattgtaga aaatgtggca
     3901 aagaaataac agatgaaagt aagtattgcc agttttgcgg agcactacaa gaaacagatg
     3961 agacaactgg agcagacgca gtaaagaaaa gtactgagca gacaattaat gaaatgaagg
     4021 aacaaatcgt acatggacaa gagacaggga agctgtattt gattattggt tggatttcta
     4081 tggtagtatc gttagcattt attcctgttg tatttggtgc tgttgctgtt attatgggtt
     4141 acttatatcg agagaaagat gaaaaaatgg gtacgatttt gatgatagca ggggtagctg
     4201 gagccatctt tggtgtactt ttagggatgt caatgtactg ataagaggga taagagcaaa
     4261 ttattttgga gataggagat aaaaagttga ataaagtaaa tatgaacaag aagtttgtca
     4321 ttataggtat tgttgtcttg gctattatag gtatcatttt ttcaaaaatg acaggtacat
     4381 ctggattaga gaagtcatcg aaaccattgg taacacagat tttacaagaa cagtacagtt
     4441 taaatcggac atgtgatgat gtgacgatta ctcatgaaaa tggagataac acatatcgag
     4501 ctaaagctat attagacaat ggaagtgcta tcaacataaa tattgagtac tatccaaaga
     4561 aagaccgtat atatgtagag ataccatata ctgaggtgtt aatgttgaat tgaaccccac
     4621 cccattgatg attgaccgta ttttgcatgg aaaccttggg aggcataagc taactagggg
     4681 ttcctttttc ttttcttttt ttggaatcta tttaagtgaa atgtatctga aatgaaatgg
     4741 gagtaagtgt tataatagtt aatacataag cgctttattt tatttcagat ggtgaatagt
     4801 ataaggaatt tttttgatat tattacagtt tatcttacta taaagtacta ttttttaaag
     4861 caataacaga tgaaaatttg attcattcat aacaacatgt acagctatac cacctacttg
     4921 gaataactga agaaaatgta cgaaaaacca tttcaagtaa accccaaatg attgttaatg
     4981 aactacaagg gtggtgaagc ttcatgagtc tccacagaga tgctccatca aaagttccgt
     5041 caagctcatg aaaattgtga aaaacgtttt gaataatatc aaagaaagag ggaatggaaa
     5101 tggcacacaa tgaacgtttt aaacaagaaa ttattcaaga actcgagaaa ggagtacaac
     5161 accaaaaatt aatatcaaag accgcccaaa aatttgatat ctcactggct aaggaggtta
     5221 aacttcatga agtttaacct gctagctcta ctaaccgtgg taacgctcgt ttttgttggt
     5281 tgtactaagg ctcctgattc cttgaaaaat tcttctaaca gtgcggaggt aaacgtttcg
     5341 acctctacca gtgaagcggt acagtgttca caagaaacta ctagcttgcc agaagagcta
     5401 cttggagatt ggcagattaa taataaggat gctggtatag agatatcaga cagtaaagct
     5461 gtattccgta taggaaggtc tcaagtagag tcaaaggact attatgtaga tgtaaacaac
     5521 aaaatgcttt tacatgtcta tttttctttt gagtatgatg gtgtgaaata cagtgaagtc
     5581 tctattgaaa gaactggcta tagtgaattt attagcaaaa gcgtgggaat tttaactatg
     5641 catactaaag atggacaata tataagtttg ggtgtgaaga aagtttccgg tgagaataat
     5701 gagacagaag cagttacgga gcagacgatg gatgaaagaa ttgattcatc tgagagtaat
     5761 gagacagaag tagatactgg gaagacgata gatgaaagaa ttgattcatt catcgaaaca
     5821 tacacagcta taccacctac ttggaaactt gtaaatgaag ataaaggaat cacgataaat
     5881 actgatacag agatttctac agatgctcaa tctttatcag atttattagc caacctaaca
     5941 ggaatcagta atgcggtgac agagcaagtt ggtaaaaatg ttccacttcg gttacttgaa
     6001 aatgggaaaa aagattattc tgtggtattt aagaacggac aggtaactga ttacaacgaa
     6061 gtatttaaaa aagttattga ataatgcttc ttcaatcttt ttcattcatt taccccaccc
     6121 cgttgttata taatcgtatc tcaagaggaa ccattagaaa gcataggcta actaggggtt
     6181 cctttttctt ttgttttttt ggaatccgct taagtgaaat gtatctgaaa tgaaacgaaa
     6241 ataacgtgat tttttactgt aatatcaagg ttttagctgt tttagatacc atgtaaccca
     6301 aaatagttta ttgagaaaat aggagagtat gtgatactaa ctagtaggga aaaatgagat
     6361 aaaaagtaaa tggaatcatt tcagaaataa gtgaaataag acaacgtaat aggtataaca
     6421 tgactattac atagttttag acatgaaata ttactacaaa atttgtctaa ttatgtcatt
     6481 atttatggag gagttttata acgatttttg taataaaaag acgagaagga atgcatacat
     6541 gcacatcctc tcgtctttta actatttact ttaggaatta caacaattgc tgtaattcgt
     6601 cacctattcg ttcaactatt ttcgtgacta aagcatttcc cataaagaat aagcgcattc
     6661 tatctgaaac ttctactatt tccccggagg ataatttttt atactttgtc caatcatcag
     6721 gaaagtcttg tagacgttcg gcttctatag gggtcaacaa gcggtaacga ccatctattt
     6781 ttaataaatg tgtagatcta ttcacagaac cttctgaagt tagcatcgtc cgtccgggca
     6841 tatctaaatc atcataagga gacataccac cttcggagaa tgtataggta tgaccttcag
     6901 cagtgactcg ctctattttt ttaggtccac gaagatattc gaattttttt attttacttt
     6961 cgtcggtcaa gtaatacttt tcagctactt catcttcagg ttgaatgatt tctcttaatg
     7021 gagtaggttt ttctttatcg tatggttccg tatctatagt aaagtaacgt ccatgtctca
     7081 ttaccccagt attgtatact tttccagtaa aattatctga tatctctact atgtcattgg
     7141 ataacttata cgacgcttgt ctacccttat aaggagtctt gatgattgga aattgtcgag
     7201 caaaaagtcc ttcactgaat atatactcat cataacgatg ttcttcttca ccaagtattt
     7261 tttgttcgaa tcgtgcatct atcttgttcg caaaaccagt atcatttttg taaacgaaaa
     7321 aaaagacacg gcgtctacgt tgtctacggc cataatcagc agcattgata acacgccatt
     7381 ctacggaata ccctaaatca ttaaaggaag caagcattat agcaaaatct ctgccacgtt
     7441 gtttagaagg agatttaagt agacgatcaa cattctctaa aatgagatac tttggtttta
     7501 tgtggttcgt agcacggatt atttgccaaa atagaactcc ttttttacct tctatcccca
     7561 tctcatgttt tttactacgt gcaactgaat aatcttggca cggaaatcca ccaacaatga
     7621 tgtcagcgtc catttgctca aattcttcat ttgatatttc ttctatattc ctgttgatat
     7681 tttcactatt tgggaatcgg tagtcatata cttcaaaagc atcttgtgat tttcgagaag
     7741 gctcccattg gtttgcccat ttcgttttga ataggtcggc attagaatgc tcgagtccaa
     7801 ctctgaaccc ccctacaccg gcaaaaagtt ctaatacatt tagtgtttca ctcatattgg
     7861 cgaacatcct ttcggtttta acttaaatgt attatacaat aaataaatcg atttgaaaag
     7921 atcgaaatca aaaatgctat gatttactta aggagtttct caaggttatt ctgtaaagtt
     7981 gaaacaacat caaatgattt tgtatagact tggtttctat gtaaccatgt agtatagttt
     8041 tatataggaa taaacgtagg atggagtgac tgaacgaatg tctgaatact taacaaaaga
     8101 gcaagtacat aatagagcta aagaagcagt ggggaagtct atgatagaat taaataatgg
     8161 aattcccctt ctcaatagta aaaataaaaa ctctatagga gacatatttg agtcttggtt
     8221 cggcaaggta aaggatagcg agagtaggcc agacctggaa gaagcaggtg tagagttaaa
     8281 ggctacgcca ataaaaaagt tgaaaaacgg tcagtatagc gctaaagaac gattagtgtt
     8341 gaatatcatc aactataatg aggtcgctga ggaaacattc gaagaaagtc attttttgta
     8401 taaaaacaac atcatggaaa tcgcatttta tgaatactta aaagatacgc cgaaagacca
     8461 gtggactatc cgtgaagcta ttttatatga gatgtataaa aaccctgtag attttgaaat
     8521 catacgacaa gactgggagc ttataaagaa atatatttta gatgggaaag ctcatgagct
     8581 aagtgaaagt ttgacgaact atcttgctcc atgtacaaaa ggaactaatg ctgtgaagtc
     8641 actcaggaca cagccctatt cagaacaaaa agctaaacaa cgggcatact cattgaaatc
     8701 aggatacatg acatctattt tacgtaaata tgtattaggc gatgaaaaaa tcgattcaat
     8761 cgtgaagaat acttttcaac ttgaagaaaa caacttagaa gatttagtat tagaacattt
     8821 tataccatat gtaggtcaaa ctgtacaatc tttatgtgaa aaattcacaa tcaagacatc
     8881 gtatcaagct aattatagaa tcgtttcttc aatactaggg ttatctggta ctgtaaccaa
     8941 aggggaacct ttcccaagag tcgaagaatt tgaaaaagcg tctatactag ttaaaaccat
     9001 atattttgat aaaagtggaa ataacaaaga aagcatgtca tttccatcat tcaattttat
     9061 tgatttgagc aaacaggtat gggaaaaaga agatggggaa ccaagcagtg attggcataa
     9121 ttttttatta gatacaagat ttctttttgt agtctttaaa gaagaagatg aaggtgttgt
     9181 attcaaggga gctaaatttt ttagtatgcc agaagaagat atagaagggc cagtccgtaa
     9241 ggtatgggaa gatactgtgt ctaaacttcg agaaggagta gagttagaag ctattcctga
     9301 taaatctact aaggatggat ggaaaatcaa aaataatttt ataaagaaaa cagacaaaat
     9361 gatttgccat gttagacccc atgctagcca acgagattac tctgtcgatg gacgctatgc
     9421 ggataaactt cctgtacccg caaaatggat taataaacca gacaacgata attactcgga
     9481 tcaatggatg accaaacaat gtttttggat aaacaatgat tatataaaag agcaagtaca
     9541 agatttgata tcataaaaaa agaggaagac tactcctctt ttttttgtgc aatataatct
     9601 aatatcacag atgtgcaata atttaagttt ttcaaaatat ctttttccca aaagtgtata
     9661 ggtacccaac caagttttat gagagactga ttattccttt catctctcct catattttct
     9721 tctatttttt caatccagta gtcccgattc cgtttcaact tttgttttct attgtcccaa
     9781 tcgtgtccgt gccaaaactc tccatcgacg aaaattgcta gtttatattt ggtaactgct
     9841 atatcgggag agcctggtaa ttttttatag ttttttcgat acctgacacc ttgatgccat
     9901 aactctttgg ctagcagact ttcagctttt cctcccttca agcgtacatt tgccatacgt
     9961 tttgatactt cgggagtggt cgatagttgt ctatgcttca ttgtttttta cctccaatgt
    10021 tctaatagaa tcaagaaatg ttagtacaga gatatgattt atttaagata tcactatcat
    10081 aaatatatag ctaaatgaaa aaagttgcga atatggtctt atagaaatag taattatttt
    10141 cagttctcag cggtcttatt gattccgttt tattgatttt aaatgttatt cagtgaccct
    10201 ttaacaggtc aatttttatg tttttgtctt cgtatgactc agtttgatat ctagtgatat
    10261 agtgatttga gtctacatcg aacaatggaa taatccaata aaaaatgatt tagaatatca
    10321 aaataacaaa aaagaggact acctgtatta gtcctctttt tgaccagatt ttgaccagtc
    10381 tattttacat tctaatatat tcatatgagc taattttatg agtaaatggt aagtttttcc
    10441 ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc tatatggaag ggtctgtggg
    10501 gtaatactta aataaaggct aggaacctga taacatcaag gttcctagcc tttttataat
    10561 aaaatgaagc gaaaataatt tcttttaaag agccgagatt atcagctata taagagtgaa
    10621 tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg tagctctgct tatatgtttc
    10681 gaatttaaga caaagagttg gcaaaagatg ttgataagga agataagata actgtgttat
    10741 ataaactagg taactaactg taacaagcta attttaagag attcttatta gaagacgttt
    10801 aggaatataa ctaagaaaat gattatacag gtaattgtca tttcaataat gttttctaat
    10861 tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg gaaaaaagtt cgtacgatag
    10921 tgaagagggg caaaatttcc tactaatatt cccactaaca agagataaaa cggccaggtt
    10981 ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa tcccatagat taacaactga
    11041 acaattttta caatgaaaaa attgttctgc cattactcaa agtctcttcc tccttgtatc
    11101 gtttatttgt ttcattttat cattatgata ggcgcttgaa caatccattt tgtattttat
    11161 tgagtactct tggataaact accagcgatt atcatattta tttatgttac tgtaagccat
    11221 catataaaag taaatgagca gatcatagaa atataaaaaa gaactaaaat tgatctaaat
    11281 tatgtgcagc ttgactacgt accaatctat attacttact agattggtcg ttaatctatt
    11341 cggatcgaat gagcaaaata tttttgtgaa tgtccatcct tgattatgat tccaaaaaga
    11401 atcattggga ggctgtggcg taaatattat actataagcc agaaatacct atcattctga
    11461 acttttttat ttaggaactt tcatctcaag gctttttcaa ttaatttgta cccctgtttt
    11521 ttggataaga tcatgtgcat tgattttttc tatctctacg ttcatctcct acttaatgcc
    11581 tatctggctc ctgagggctt ctttttaagg cgtcgtcata gtcttggctg aggatttctt
    11641 ggtaatctga gggaatggct tcccaagtga gcaggcgaac gttcatgacg tgttttcctt
    11701 tgtgtttgat tacagcatgg tttttctctt tttctacctc tccagcgtga aaagacagcc
    11761 atgtaccatg gccaaagata tcattacata cccaacgttc gatggaattg tccacgtatc
    11821 ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa aggattgaat cgatcgatcg
    11881 tttcattttt gtattgataa ttgataagaa aaagatagac caaacaaaag acgagcagaa
    11941 agaaagatag tgtctttttg agtgagaagg gttgttttga tgaataagat ttcttgttaa
    12001 caagaggttc atctggcaat gttggttcta ttgattcggt agctgattca atagaaggaa
    12061 aatcaatcgg cagacctaaa gtatgatgta aaagtgacag catttcaagg tctggcattg
    12121 tacgagaatt ttcccaacta gaaatcgttt tatcggaaac accaattttt tcgcctaatt
    12181 ctttttgcgt aagcttacaa tttctgcggt aagttcgtat tttttctccg gtgttcatct
    12241 tcttctcctt tcgattctac agtatataga tttattctac atgacctata cacttgatag
    12301 atacgttttt ttgatgaaaa acggtatgat tttatcaaag aatcatttaa gtaagggaga
    12361 gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa tcatagtgac cggtatcatg
    12421 agtggttttg ccttggttag tataaataaa caggtactct atcggacaaa tgaatttttc
    12481 catcaaacag atacttatac tccgattcga ttgccacaac attttattgg gcaagaagaa
    12541 tatgagcagc taatcatggt atttgaaaat gtctcatcag aaactgagct ttcttatatg
    12601 aaaaaaacag taaatgaatc caatgaagta gatacatctt ggaacttcaa gatacagcca
    12661 aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat cttctcacga gaaggtaccc
    12721 tattctaata cctcctttcg cgtaacgtcc attgaagaat cgatcaaaat aaaagatttt
    12781 gaaggtgaat ttttcttgat gacgagagaa aaagaagcct atgaacggtt catttcacta
    12841 ttcactgatc gattcaatca agcatttcac actgattatg ccactaatat ttttgaagac
    12901 tttgaaaata gctattcgta ttcggttatt tcaaatacag acgtatataa ttatcgagtg
    12961 tatttaacta ttggattgat tcttttattc cttttgttat catccttttt cttattgatg
    13021 aataaagaaa tcagtttatt gttgaataat gacttttcat tacatcaagt attgtggtgt
    13081 ttattgggga aaaagaaaat cattattaca ttcataattg caggaatcat tggtggtttt
    13141 atttttatgt attataagga gcaatttctt tatttgttcc agatgtacgt tttcttgttc
    13201 agcggaatat atggtatctc tttgatttct cttttattag ttgaaaaaat cgcttcgtta
    13261 agagaagcgt tcaactatat ccaatacatt ctttttacat ttggattgtt gttttctctt
    13321 cttttgaccg ttgccaatgt cgaattagca actatcgttt tttcttcttc acagttagct
    13381 gtttcccatg aaccaccaaa agaaatcgct ggtgaggact atcatgtatt ttatccagta
    13441 accatcggga aaaaccacgt ggattttatt tattccaatc gttttgcatc agcagcagat
    13501 caggaattat atgaaacttt gaataaaaac ggaagtatat tagtcaatgt atcagattac
    13561 ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa taaatctgaa ttatcttaag
    13621 aaatttcctt tgattgatgt ctctggaagg ttgatccaaa taaccgaaaa agaaacgcac
    13681 cctgttatat tgatccctga acgatataga catacagatt taaaaaatga cctggcgcaa
    13741 ataacagaat attatcaaga aatcagtgaa aaagaaccga aatttctatt tataaaaaac
    13801 aatcaaccca tttatacatt tattccttct atcccctgga tcgaacatta tccggttgtt
    13861 gaaatactga cattgaaaaa cagtatgtat ttggaaagaa atattctcag cggagaaatc
    13921 tatccaccgt taaagatcaa gataggctct ttgtcgaaag aaaggttatt tgagctgatt
    13981 gaagaaagtc agttacgtga taacttgcag ttatcatttc cgtatactca gactgaagag
    14041 atagctgtca aagtgttaag cagatcattt cattacttga tacagatatt tttactcact
    14101 ttcttttctt tcttcttgtt gagctatgtc atgttatgca tatattttgt ctacaattgt
    14161 cggaaaatcg ctatctttcg ttcaagtggt tattcgctct ttgaaacgta taaagatttc
    14221 ttcatgatga acctcatcaa atggggaacg acaagtgtaa tcttcctttt tcttatagaa
    14281 agagagccta aatatctttt taatattttt ttctttagct tcattgattt tattctttca
    14341 gttgttttta ttttgtctac agaaaagaaa agtcagttat tgctgatgaa tggaggttaa
    14401 caattggagc cgttgatagt gataaaaggt gcgaaaaaaa tcattcgaaa caaaacgatt
    14461 cttcatttgg atcgtttcac tgcggatgct ggggatcttg tgactgttgt tggagaaagt
    14521 ggagcaggta aaagtacgtt attgaatata ttaggattga atgatcagtt cagttcagga
    14581 caattctatt tgttcgatca agagacgaaa caattaactg ggagagagaa gcgcttgata
    14641 aaaagaaata ccatatcttt tctttttcaa gattttgggt tgattgaata tgaaacaatc
    14701 gattttaatt tagatattgg attgaagttt tcgaatttaa atgtttcaga taagcgaaca
    14761 gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga agctaagtac gcctatctac
    14821 acattatctg gtggtgagaa gcaaagaatc gcctgtgcga gagtcttatt gaagaatagc
    14881 aaaatcattt tagccgatga accgactggg tcattggata aaagaaataa ggataaagta
    14941 gttgaaatct tatcgcaaca agcttcccaa ggtacgttag ttatcgtagt cacgcatgat
    15001 ccatatctga ccaaaatagg aaacaagaag atcatgcttt aaaaatcaaa aaggaaagga
    15061 tgtcgtttta tgaaaaaagc agttattatt tgtgcactag ggataggttt tgttggagga
    15121 aacgctatgt catctgaagt ggaagcaaat tcagttcacg gtgaaatcta ttacaaatct
    15181 ttaaagccag gagagattca ttttgatgct aaaggtaata ttattaagtc aaatttacca
    15241 gtagaccggg aaatttctag aacagcaatt aatcatagct ctggaagatg ggtttattat
    15301 agtgatacaa cttgtacgag aaaaacaggt cattctaacc actatagtcg tgtctataat
    15361 cgtcacggtg caaaagcgaa agttggaaac tctattcgat cagcaaatgc aacaaaagga
    15421 gtatgggcct atgcaagtgc gacaggaaat agaactgaca cattctcatg ttggtacaat
    15481 ccaaccggtt ggtattaagt atccaaaagt ttatataaaa tagaaaaaac atataatgaa
    15541 acagcgttaa aagttatttt tggt
//
LOCUS       DAZKCZ010000058        15515 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000058 DAZKCZ010000000
VERSION     DAZKCZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.058"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..597)
                     /locus_tag="ACG8KN_001678"
     CDS             complement(<1..597)
                     /locus_tag="ACG8KN_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188737.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
     gene            790..1821
                     /gene="queA"
                     /locus_tag="ACG8KN_001679"
     CDS             790..1821
                     /gene="queA"
                     /locus_tag="ACG8KN_001679"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9188738.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            2440..5079
                     /locus_tag="ACG8KN_001680"
     CDS             2440..5079
                     /locus_tag="ACG8KN_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9188739.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(5247..5945)
                     /locus_tag="ACG8KN_001681"
     CDS             complement(5247..5945)
                     /locus_tag="ACG8KN_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9188740.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            6163..7308
                     /gene="tgt"
                     /locus_tag="ACG8KN_001682"
     CDS             6163..7308
                     /gene="tgt"
                     /locus_tag="ACG8KN_001682"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9188741.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            7405..7785
                     /gene="yajC"
                     /locus_tag="ACG8KN_001683"
     CDS             7405..7785
                     /gene="yajC"
                     /locus_tag="ACG8KN_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9188742.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            8090..10687
                     /gene="adhE"
                     /locus_tag="ACG8KN_001684"
                     /gene_synonym="adhC"
     CDS             8090..10687
                     /gene="adhE"
                     /locus_tag="ACG8KN_001684"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9188743.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            10916..12022
                     /locus_tag="ACG8KN_001685"
     CDS             10916..12022
                     /locus_tag="ACG8KN_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9188744.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            12039..13063
                     /locus_tag="ACG8KN_001686"
                     /pseudo
     CDS             12039..13063
                     /locus_tag="ACG8KN_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            13069..13548
                     /locus_tag="ACG8KN_001687"
     CDS             13069..13548
                     /locus_tag="ACG8KN_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9188745.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            13564..14118
                     /locus_tag="ACG8KN_001688"
                     /pseudo
     CDS             13564..14118
                     /locus_tag="ACG8KN_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            14174..15513
                     /locus_tag="ACG8KN_001689"
     CDS             join(14174..14615,14615..15513)
                     /locus_tag="ACG8KN_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFP9188746.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         5103 a         2726 c         3339 g         4347 t
ORIGIN      
        1 cgcttcatcc atgtcatggg ttacaaatac gatcgttttt cccaaacgct cttgtaaatc
       61 tttgacaagg tcttgcaagg aatcacgagt gattggatca agcgcaccga atggctcgtc
      121 catcaagatg atatcttgat tcgctgcaag ggcacgaacg actccaatac gctgctgctg
      181 accaccagat aattcatgtg ggtagcgatc taacatctca cggggcaatt ccacaagatc
      241 aatcattttt tcagctgttt tattccgttc ttcttgactg actttcaaca atttttggac
      301 aagagtgata ttttcacgga tcgtcatgtg aggcatcaat ccaatacttt ggataacata
      361 gccgatctga cgtcgcaatt ccactggatc aatttcttgg atggattttc catcaatcaa
      421 aattttccct tgtgttggat cagtcattcg gttgatcatc cgcatcgtcg tcgtttttcc
      481 gcttccgctc gtaccgataa aacaaatgaa ctcacccttt tcaaatgaaa gattgacgtc
      541 ctctacagcg acttttccac ctttataaat ttttgacaca ttttgaaatt caatcatggt
      601 gccacctctt tttctatttt caatgaagaa ctatctatta ctagtatgcc taaaacaatg
      661 aaaaaataca aatagggtag tttttttatt taggaaaaca aaaaatagaa gtgaaaaagt
      721 gctgattttg ccaacagaac gaaaaacatg atagaatgat gaagcacatg atagaaagtg
      781 agtgacgata tgttaacaac agaagatttt gattttgact tgccggaaga attaatcgca
      841 caaacaccat taaaagaccg cgatcattct cgtttacttg ttttggatcg gaaaactgga
      901 gagatggaag ataaacattt ccatgaaatc attgaagagt tacagcctgg tgatgcgcta
      961 gttatgaata atacacgagt tctgcctgct cgtttatatg gagaaaaacc agatactggt
     1021 gcgcatttag aagtccttct tctaacgaat acacaaggag atacgtggga gacattgatc
     1081 aaacctgcga aacgtgccaa agttgggaca agaatctctt ttggtgatgg cagattgcaa
     1141 gcagtagtga aagaagaatt ggaacacggc ggacggatca ttgactttga gtatgaagga
     1201 atttttctag agattcttga atcattggga gaaatgccat tgcctccata tatcaaagaa
     1261 cgtctggaag acccagatcg ctatcaaact gtctatgcga aagaaaatgg ttctgctgca
     1321 gcgccaacag ctggattgca ttttacgaaa gaattacttg atgagatcga agcaaagggt
     1381 gttgaattag tctatctgac actacatgta gggttaggta cattccgacc tgtcagcgta
     1441 gataaaattg aagaacacca tatgcatagt gaattctacc gtttgacgga agaagcggct
     1501 gagcgattga accaagtacg taaaaatggc gggaaaatcg tggctgtcgg aacaacttca
     1561 attcgaacat tagaaacgat tggaacgaaa tttaatggag aaatcaaagc agacagcgga
     1621 tggactgata ttttcattac gcctggctat gaattcaaag tggtagaagc tttctcaact
     1681 aatttccatt tgccaaaatc gactttagtc atgttagtta gtgcctttgc aggaagagaa
     1741 ttgactttat ctgcttacca gcatgcgatt gaagagcgct atcgtttctt tagttttgga
     1801 gatgcgatgt tcgtgaaata aggttatgaa aagccgctag ttttgaataa caaaaaagct
     1861 ttacaaagat tttatgacaa agaaaaagcc gagctctcga taagctcggc tttcttgtac
     1921 ctcggaagga ttcaaataaa cagaaatgat tgtaactttc catcatacat gcaaagcacg
     1981 gtgttgaatt tcttcatgtt aaacaaaaat tttgttttcg ttacatcttc aacatttgaa
     2041 acatctcttg ttcctattca attttcagac gtttacgtta ttcaattgtt acgtatcgca
     2101 ttacttcttg tttcggatac ttcgtgatcc aaacatccat acgcattcac tatttatagg
     2161 tttgtactct acacgcatgt ctgggtgaaa cctgtacttc taacaacttt gtttgcttac
     2221 actgttctat cgagaaattc ttctgtctat ttgatggtat cctccttcct tacatctata
     2281 taatagcatc ctttggtagc gtttgcaaat ataagcactt taaattttac gctttcttaa
     2341 caaataatcg ataagccctt tatttgaagt aaataaaaaa aggatgttat agtaaaataa
     2401 gtaccttttt ttgtacgaag attttaaagg aggaatagga tggaagaata caagcagaca
     2461 atagaagaag taaagaaggt aacagaagca gatccagata ctgggttgtc ttcacaagaa
     2521 gtggatgaac ggcgaaaaaa gcagggattg aataaattcg atgaggcgcc gaaagaatcg
     2581 atgatcaaaa aattctttcg aagcttgtcc gattttacaa cgatcatcct tcttgtagca
     2641 gcggtgattt ccttttatac cgcatttgct acagaacatg gggatttatt tgaaggactg
     2701 ctgatcatcg ctatcgttgt gatcaactcg gtattggcga ttgtccaaga aggaaacgca
     2761 gaaaaagcac tggaatcctt gcaagatatg aataaacaaa cggcaactgt catacgagac
     2821 ggcaaagtga ccactgttga gtcagagcaa ctggtagtgg gtgatattct tgttttagag
     2881 tccggtgatg ctatttctgc cgatgcccgt ttgatcgaag catcacaatt acgtgtagaa
     2941 gaatcggcgt tgactggaga aagtgaagca gttgaaaaag atgcagcatt tattgctgaa
     3001 gaagatgagt cattaggtga ccaacttaat atggtattta aaggatgtac ggttgctgca
     3061 ggtcgtggga aagcgatcgt cactgccatt ggcatggcta cagaaatggg taagatcgcg
     3121 gatcttttga acgaaaatac tatgcaaaaa acacctttac aggtccgatt gaatcagcta
     3181 ggcaaacgaa tcagtatgat cgctttagcg gctgcggctt tagtatttgt gattggtgaa
     3241 ctgcaaggag aaccactcct tgagatgttc atgacttcga tttccttggc tgttgcagct
     3301 gtaccggaaa ccttgactgt tattgttacg ttgacacttg catacggtgt gcagaagatg
     3361 gcgaggaaac atgcgatcat ccgacagttg ccagcagttg aaacgctagg aaccgctaat
     3421 gttatctgct cagacaaaac aggaacgctg acacaaaatg aaatgcgcgt ccgccgggta
     3481 tggagcaaag aagatgaagt aacgaatatc gaagattcaa tgacgaacag cgcgatggaa
     3541 atattgaaaa tggctgcact atgtacagat gtgacggtcg agcaagaaga tgacgacctt
     3601 gttatcaaag gtaatccaac agaagtagcc atcgtaaggg ctgttgaaga gaattatcat
     3661 acaaaggcag aattagaaga aaaatatcca cgagtaaacg agcttccttt tgattctgaa
     3721 cgaaaaatga tgacaacagt tcaccaaatg ggcgaaaaat acatctcaat caccaaagga
     3781 gcattcgatg ttcttgctcc gcgctttagt tcaggcgatg tagaacaagc ggcgatcgtt
     3841 aatgatcgct ttggtaaacg ggcactgaga gtgattgctg ttggttatgc tacttatgac
     3901 gaagaaccgc aagatatttc ttctgaagca ctggaaaaag acttacgttt gattggtctg
     3961 atcggaatga tcgatccacc acgtccagaa agtaaaggag caatcaagcg agcgaaaaag
     4021 gctggcatca aaacagtcat gatcactgga gatcatgtgg tgactgccag tgccatagcg
     4081 aaagaattag gtatcttgaa tgatccgtca gaagcacttt caggatcaga actgcatcaa
     4141 ttatcagacg aagaattaga tgcgcgtgtc aaagctctct ctgtctatgc tcgggtaacg
     4201 ccagaagaca agatccggat cgtcaaatct tggcaacgta cgggtgcagt cgtagcgatg
     4261 actggggatg gtgtgaatga tgcaccagcg ttgaaagcaa gtaacgttgg gtgtgccatg
     4321 gggatcacag gaacagatgt ggcgcaaagt gccgcagata tgattttgac ggatgacaac
     4381 tttgcgacga tcgtagacgc tgtcagtcaa ggacgttcgg tttatcagaa tattcgtaaa
     4441 gctatcaact ttttgctaag ctgtaatatt tcggagatct ttattgtttt gattgcgatg
     4501 ctgttaggat ggggagcacc atttacagct gtgcaactac tgtttgtcaa tgtagttgct
     4561 gatggacttc caggatttgc tttaggaaga gagccagcgg aacacggcat catggatcag
     4621 ccacccattc cgaaaaatga agggatcttt gctcgaggtt tattgcaaaa aattgccata
     4681 aatgcagggg tcttcaccat cgtaaccttg tttggttatt atttaggtag ttatgtcgat
     4741 actatcagcc catgggtaga cgcaagtcag catgtcggtc aaactgttgc attcttgatc
     4801 ttagcttatt catcgattct gcatgtgttc aatgtacgca gcagccaatc gatcttcaaa
     4861 gtcaatttag cgaccaataa agctttggta gaaatggcgt tactggcatt agctattaca
     4921 acggctgttg ctttgctgcc atttacgcaa gaactattcg gcctagtaca catcagtttg
     4981 aatcactggt tcttggtagg gattttatct atcgtaccaa tcgcggtgaa tgaactgatc
     5041 aagttccatc gattaccgga agcagaagaa gaggaatgat taactgtttc taagttgatt
     5101 ttctttttac tggaactata caaaaaggag gtgtgacatg aatgttgtca cacctccttt
     5161 ttgctaatgg gctgaatgtt tcttttactg cctctcgata aacggtgttc tcctgccacc
     5221 ccctcggtca caagtcacac tctcttttaa atatcttttg gcaattgttc ataaaatgct
     5281 gcttttgttg cagaaatgta tgggctgagc catagataac caataccagc agttgctaat
     5341 gctaagatat gccagccgat aaatgaaaga tctaaccaga aaagttgtcc tttataacca
     5401 tccatcaatt ttcgacttgc agtgatcgta tccagtactt taggtgtttg tcctgtttct
     5461 tggacgatat cgtagtaaat aaaatagctt tgagaataag aatagctttt aatgattcca
     5521 ggaataacta atagcaaacc ccataatgtt gtaaagatcg agatcagcac agctaagctg
     5581 atcactccgc cgataaacaa acctttgaat ccacgaaatg catctttgaa tggttcgatc
     5641 tgatcttttt gtccacgtaa aatatcaaga taagtccaag aaataccgct catgaacaaa
     5701 gcgctaatga tgctggaaat aattccgcca ccgcctcctg aactaccgcc tcctgaagtt
     5761 gtcgcttctg agacgctcgc tgatccatca tatatggtgg tggcaaacaa gataatagga
     5821 atcataataa gaagaatagt agcaatacca atcaatgtag ggattagatt tagtaaaaca
     5881 gcttgtcccc aacgtccttt tagactctct tttgcttggc gttttaattc tgcagaagat
     5941 tgcatgattt ttccctcctt ttctttaata acaccaatat accatgtatc aaaaaatatt
     6001 tctgtcataa tgtttttctt ttttttgtta ttcataaaaa aaggataaaa aaaagaatat
     6061 taaataaagt agaaggaagc cgagggaact tgcaaaaagc acttgaaacg attatgatgg
     6121 tgggagttgt caagatattt tatatagaaa gtagggaaat gaatgacgca acctgccatt
     6181 cgctatcgtt taatcaagaa agaaaaacat actggcgcgc gtttagggga attgatcacg
     6241 ccacacggaa ctttccccac accaatgttc atgccagtag gaacattagc tacagtaaaa
     6301 acaatgtccc cagaagagtt gaaagaaatg ggtgctggcg ttattttaag taatacatat
     6361 catttatggc tacgccctgg tgaagactta gtagaagaag ctggcggact gcataaattt
     6421 atgaactggg atcaaccgat tttaacagat tcaggcggct ttcaagtttt ttcattaagt
     6481 gatatgcgga atatcgaaga agaaggcgtg catttcaaaa atcacttgaa cggttcgcgt
     6541 atgtttttat caccagaaaa agcaatcaac atccaaaata aattgggctc tgatatcatg
     6601 atgagttttg acgaatgtcc accattcgat gaaagttacg actacgtgaa aaaatcagta
     6661 gaacgtacgt ctcgatgggc agaacgagga ttgaaagctc atgctaatcc agaccgtcaa
     6721 ggattatttg gaatcatcca aggagcaggg tttgaagatc ttcgtcgtca aagcgcgaaa
     6781 gatctagtcg ggatggattt ccctggttac tcaatcggcg ggttatctgt cggggaacct
     6841 aaatcagaaa tgaatcgtgt attagaattc actacaccgt tgatccccga aaacaaacca
     6901 cgttacttga tgggtgtcgg tgctgctgat tcattgatcg atggtgttat ccgtggagta
     6961 gacatgtttg actgtgtctt gcctactcga atcgctcgaa atggcacatg tatgacttct
     7021 caaggccgtc tagtagtgaa aaatgcgaaa tatgcccatg atttccgtcc aattgatgag
     7081 aaatgtgatt gttatacatg taaaaactat acacgtgcct atatccgcca tttgatcaaa
     7141 tgtgatgaaa catttgggat tcgtttgact tcttaccaca atttatactt cttgttgaat
     7201 ctaatgaaac aagtacgtca agcaatcatg gatgataatt tgcttgaatt tagagaagcc
     7261 ttttttgaag aatacgggtt caataaagaa aacgcaaaaa gcttctaaag gaaaatcttt
     7321 taaaaattaa gtaaaaaact agtgttatca gcatttttct gctaaagtgt tttttgtaga
     7381 tacagaatta gtgaggtgaa aaaaatggga acattaccaa tgcttgtgat gtttatcgcg
     7441 ctattagcaa tgtggttttt tatgtcacgt tcgcaaaaga aacaacagca agaacgtcaa
     7501 aacttgttag atagcatgaa accaggtgat tcagtcgtaa cgatcggcgg attacatgga
     7561 gttgtttcag aaatcgacaa tgacaaacgg acagttgttt tagactgcga agggatcttc
     7621 cttgagtacg atcgtgcagc aatcaaaacg gtcaaaccag ggactgtcgt aacaaatgat
     7681 gcaacgacta ctgttgaaac aacagaagaa ccagctgaga cagttgaagt aaaaactgaa
     7741 acaacagaaa cgctggaaga gacagaagag aaggaagaaa aataagtatc aaagcttatt
     7801 taaagacgag gtagggcaga aagatgtcct acctcatttt ttattcaaaa aagaaagaga
     7861 taagagcatg ggatatattg ctcaatattg aataatcggg tgtgtcatat aaataataat
     7921 aaataacttt atatattttt ctatacaaca cctgtaaata aagcaatttt atatttaaaa
     7981 ataactgtga aaaaaatcac aaaaaaagtg tttacataga aaaataaata ttgtatgata
     8041 tacatgtgaa ttatttaaca aataaccatg acccattagg aggataatga tggctaaagt
     8101 aatagaaaaa gaaaaaacga agacaattga cgtacaagca atgattgatg atttggcaga
     8161 aaaagcaaat gcagcattga aagaaatgga aagctttgat caagaaaaag tagaccatat
     8221 cgtgcatgaa atggcaatgg cagctttgga tcaacatatg ccattagcga agatggctgt
     8281 agaggaaaca ggccgcggtg tttacgaaga taaagcaatc aaaaatatgt atgcgtccga
     8341 atatatttgg cataatatca aacatgataa aacagttggt gtgatcaatg aagatgtcca
     8401 aaaagggttg attgaaattg ctgaaccagt cggtgtggtt tgcggagtaa ctccaacaac
     8461 gaatccgact tccacaacga ttttcaaatc aatgatcgcg ttgaaaacaa gaaatccaat
     8521 cgtttttgct ttccatccaa gtgcccaaaa atcttctgca gaagcagcac gagtggtacg
     8581 tgatgctgct atcgctgccg gagctcctga aaattgtatc caatggatcg aacatccttc
     8641 gatcgaagcg acttctatgc taatgaacca tccaggtatc gcgatcgtct tagcgacagg
     8701 tggagctgga atggtcaagt ctgcttactc tacaggaaaa ccagctttag gtgtaggtcc
     8761 aggtaacgta ccagcttata ttgaaaaaac agcaaaaatt aaacgtgcag tgaacgattt
     8821 gatcgtttcg aaaacatttg ataatggaat gatttgtgct tctgaacaag ctgtgatcgt
     8881 ggataaagaa atctatgcag cagtaaaagc agaattccaa gcacaccaag tttatatcgt
     8941 aaaaccagat gaattgcaaa aattagaaga tgcagtgatg aacgaaggaa aatatgcggt
     9001 caatccatca atcgttggac attctgcgat ggaaatcgca aaattggcag gaatcagtgt
     9061 accaaaagga acgaaaatgc tgattgctga attggaaggc gtaggcccag attatccgct
     9121 atcaagagaa aaactttctc cagttcttgc catgatcaaa gctaacaaca cggaccatgc
     9181 gtttgatttg tgtgaaggta tgttgaatct tggaggatta ggacatacag cagttatcca
     9241 ttcagaaaac gaagagttgc atgtcaaatt cggtcttcga atgaaagctt gtcgtatttt
     9301 agtgaataca ccatctgcag aaggcggcat cggtgatatc tataacgaaa tgattccgtc
     9361 attgactttg ggatgcggtt cttatggtaa gaactctgtt tctcgtaacg tctctgctgt
     9421 aaacttaatc aatgtcaaaa ctgtagcgaa acggagaaat aacatgcaat ggttcaaatt
     9481 accgccaaaa atcttctttg aaaagaactc tctattgtac ttagagaaaa tggaaaacgt
     9541 tgaacgtgtc atgatcgttt gtgaccctgg tatggtccaa ttcggttact gcgatacagt
     9601 tcgtgaagta ttggcacgac gtaaaaatga tgtgaagatc gaagtcttct cagatgttga
     9661 accgaaccca tcgacaaata ccgtatacgc aggaacgaaa ctgatggcag acttcaagcc
     9721 agatacagta atcgctcttg gaggcggttc tgcaatggac gcagctaagg gaatgtggat
     9781 gttctatgaa cacccagaca catctttctt tggtgcaaaa caaaaattct tggatatccg
     9841 taaacggaca tataagatcg acaaaccaga aaaaacacaa ttcgtatgta tcccgacaac
     9901 atcagggact ggttctgaag taacgccatt tgcggtcatc actgacagtg aaacacatgt
     9961 gaaatatccg ttagcagatt atgcattgac gccagatgtc gcaatcgtcg atcctcaatt
    10021 cgtcatgtca gttccagcat ctgtcacagc agataccggg atggatgtgt taacacatgc
    10081 aatcgaatcc tatgtttcag tcatggcttc agattataca cgtggattga gcctacaagc
    10141 aatcaagtta gtctttgact atttagaaaa atcagtaaaa acacctgata tggaatcacg
    10201 tgaaaaaatg cacaacgctt ctacaatggc tggtatggcg tttgctaacg cattcctagg
    10261 tatttgtcac tcagtcgcac ataaaatcgg cggggaatac gggatccctc atggacggac
    10321 aaatgcgatt cttttaccgc atattatccg ctataatgca aaagatccac aaaaacatgc
    10381 gatgttccct aaatatgact atttccgtgc agatacagat tacgcagata tcgcaaaatt
    10441 cttaggtctg aaaggcgaaa caacagagga attagtcgaa gctttagcaa cggctgttta
    10501 tgaattagga aaatctgtcg gcatcaacat gagtctaaaa gctcaaggag tcacacaaga
    10561 aacactagat acaaccgtag accgtatggc tgaattagct tacgaagacc aatgtacgac
    10621 agctaatccg aaagaaccat tgatctctga attgaaacag atcatcattg atgcttacaa
    10681 cggataagca aaagaaagcg tagaaaaaag ataagaaaat agatgaaaaa gaacttggaa
    10741 aacgttgttt ctccaagttc tttttttaat ttccaagtaa ctgaaagtta cttttgttat
    10801 ttaacattta ggtcaatttt agacagtttt tgttcaaaaa gcgatattta tagtggacgg
    10861 tattactgct atattaagga tataaaatga aagcgtttaa aaaggtggcg taaagatgat
    10921 tattaaagaa gagatttttg tacctaaaga attagtgaaa gaaaaaattg acctgctcgt
    10981 tgaaaacctg acaacgatca aagacgataa tggtgaattt ttgttggact ttgatggact
    11041 gaaagtagat gacaaaagct ggacagtgtg gaattggcca caaggtgtcg gtctatatgg
    11101 tatttataaa aattaccaga tgacaaaaaa tcctcgcgct tatcaagtgg tcaatgaatg
    11161 gtttgaggat cgtatggaag aaggcgctcc gcccaaaaac gtcaatacaa tggcaccgct
    11221 attgactatg gcttacttgt atgaagacac gaaagattca cgctatttgc cttatttaga
    11281 acaatgggca gaatgggtca tgaatgacat gcctcggaca aatgaagacg ggttacagca
    11341 tgccacatat ggaccggaaa ataagaatca gttgtgggac gatacattga tgatgacagt
    11401 gcttcctttg gcaaaaatcg ggaaactgtt gaatcgccca gaatatttag aagaagctcg
    11461 ttaccagttt atgatccata tcaaatactt gatggataaa cgaacaggat tgtggtatca
    11521 cggctggacg tttgaaggaa atcataacta tgccgaagca ttttgggctc gtggaaactg
    11581 ctggttgacg attgctattc cagagatcat tgaaatctta gaattatcga aagaagatgc
    11641 attgcgcaaa ttattgatcg aaacacttga agcacaagtg cgtgcattaa aaacttatca
    11701 gtctgaaagc ggtttatggc atacgttgtt ggatgatcct tcttcttacg tagaatcttc
    11761 tgcaactgca ggatttgctt acggtatttt gaaagcggtc cataaacgtt atttaccaca
    11821 agaatataaa gaagtcgctt atcgtgctat ccaaggattg cttgaacaaa tcgatgaaaa
    11881 aggagaagtt caaaacgttt caatcgggac aggaatgggg gacagtttag atttttatcg
    11941 gaatatcgga atcacagcaa tgccttatgg acagtcgtta acggtgcttt gtttgacaga
    12001 attgctcgtc tcttattgtt aaaagaaagg aaacccatat gaaaaaaaca tcactgctgt
    12061 ttgcagcaag ttcgcttagc ctaggtctgc taggtctaag cggctgcgga aataattcta
    12121 gtgtggcaac tgtcgcagat gcagacgtaa atgagatagt taccttgcgc tttgcttatg
    12181 caagcaacag ccagtaattg atgcaatgaa tgaatttggg cgtttagtaa aagaaaaaac
    12241 agatggttcc gtacagatcc agtatttccc agatggacaa ctgggtggag aacgtgaact
    12301 gatcgaattg acacagtcag gagcagtaga tttcacaaaa gtaagtgcct cagcgttgga
    12361 aagtttctct aaagattatt cgattttttc cgttccttat ctattcaatg atgaagaaca
    12421 tttctttaag gtgatggata atgaagaaat aatggagcca atctaccaat cgaccaaaaa
    12481 tttaggattt accggattga cctattatga ttctggtcag cgcagctttt atatggtaga
    12541 cggtccgatc catacgccgg aagatttgaa gggaaagaag atccgtgtga tgcaaagcga
    12601 aaccgctatc aaaatggtag agcttttagg cggttcgccc gtacctatgg gaagtgatga
    12661 agtgtacacg tctcttcaat ccaatttgat caacggatca gaaaacaatg aatttgtctt
    12721 gtatacagct ggacatggcg gggtagctaa atactattcc tatgatgagc atacacgtgt
    12781 gccagatatc atcatcatga atgatgccat caaagaacag ctaactgatg aacaggaaaa
    12841 agccattgaa gaagcagcga aagaatcaac ggtttttgaa attgaagctt ttgcaaaagc
    12901 aattgaagaa gaaaaagctt tagcacaaga agaacatgga gtccaattca atgatgtaga
    12961 taatacaccg tttcgagagg cagttgcccc acttcatgat atattcaaaa acgatcctac
    13021 ctacaaagac ctttatcaaa agatccaaga gcaaggagcg tagctgtaat gaaaatgatt
    13081 ttaaacaagg tattagagat cctgggagct gttttgcttg ttgggatggt cgctgttgtt
    13141 ttatggcaag tattttccag aacggtttta ggaaatccaa atacggtcac ggaagagctt
    13201 gttcgattcg gtcttgtctg gtttgccatg ttgtcttccg cgtatgttgt gggacaaaaa
    13261 gcacgtttag ccgtaacggt tttaagtgaa aaattaactg gcaaaaaaag taaaatttta
    13321 gaagttgttg ttcaaagttt atttctggta tttgctgcga tgatcatggt gtatggcgga
    13381 tggaatgctg tcacattgac aatggggcag atctcgcctt ctttaagtat tcctatggga
    13441 tatgtttatc tgtcagtacc tgtatcgggg gtactcatca ttatctatag cctgatcaat
    13501 ctaatcgata cattaagcaa caagccagca attcaagaaa aaatataaaa atggaggaat
    13561 aaaatggcaa tagaagccgg attagtgtta ttttttgtat ttgttgtgtt gcttgtatta
    13621 gggatgccga ttgctataag tattgcattc tcatcgatga tgactttatt gttagttata
    13681 ccgtttgatg tttctgcttt tagttcagct caaaaaatgg tgggaagtat caatagtttt
    13741 tcgctgatcg ctattccgtt tttcgttctc tcgggcatta tcatgaataa tggagggata
    13801 gctaagaaac tagtggattt tgcgatgttg ttcgtcgggc gcgtgccggg ggctttggct
    13861 catacgaatg tgttagggaa tgctttattt ggttccatct ctagctcggc cattgccgct
    13921 tctaccgcga ttggtggagt gttgatccct cagcaagtgg aagaaggata tgaccggaag
    13981 tttgcgacag cggtcaatat cgcgtcagct ccgacaggta tggtcattcc tccaagtact
    14041 gcttttatca tgtattcact tgttgctggt ggtgcttcga tctctgcttt gtttatggga
    14101 ggttatctcg taggcgcttt tgatgtgcac cccaaaagtt agactagaaa tctaattaaa
    14161 aggggtgctt tttttgcgga aatatacatt tgtttttaag aaaaaagtag tttcagacta
    14221 tttaaataac gaaggcggct acaaatatct tgcacataaa tatcaaataa atcgtacact
    14281 ggttagacat tgggtaagga tttataacta tcatggttgg gaaggcttgg ttggaggtgg
    14341 caaaagctac actacaaaat ttaaacttga tgttatagaa tatatggaaa caaatggtct
    14401 ttctatccaa gaaactgcta aaaaatttaa tatcggttca aatagaactc taagtaaatg
    14461 gatagagcaa tatgaagaag gcggtgcttc ttcacttgag agccaaaaaa ggggcagaaa
    14521 aattagtatg aattccaagc taaacattcc taaaaaactt aaagatgagt ctcttgaaga
    14581 agaagttatt cgtttaagag cagagaacgc atatttaaaa aagttagaaa ccttgattca
    14641 agaacaagat ttgagcaaga agaaatcaag gttaaaacaa tccttcagct caaaaaagaa
    14701 tttaaattga atctattact ttcaattgca caattagcga agtcaactta ttattactgg
    14761 gtaaaaaaat tagataagcc agataaatat agcaagataa agcaagaaat tacagcgatt
    14821 gtaaaagaat caagaaactc ttatggttat cgtagagtca ctttagcgtt aaagatgaaa
    14881 ggatatacaa tcaatcataa aacagttaga aaattaatgt cccaaatggg acttacctgt
    14941 caaatccgaa taaagcgata taaatcttat aaaggaacag taggaaaaat tgccaagaat
    15001 gtgttaaaac gaaatttttc agtagataca cccaataaaa aatgggtgac agacgtcact
    15061 gaattcaaga taaaaggaag aaaaatctat ctatctccta tacttgattt atttaacgga
    15121 gaaataatta gttatagtat atcaactagt ccaacatata aactgattga agaaatgctt
    15181 caacaagcaa ttaaaaaaaa gggaactgag ggttcactga ttctacattc agatcaagga
    15241 tggcagtatc agatgccaca atatcaaaaa aaattaaaag agaataacat tattcaaagc
    15301 atgtctagaa aaggaaattg cttagataat tctgtaatag aaaatttttt tggtgtgctt
    15361 aaatcagagt ttttttatcg agaaaaattc cgatcaattg agatatttca aagtaaatta
    15421 aatgagtata ttaggtggta taataacaaa aggataaaac taaagttaaa tggactatct
    15481 ccagtagaat atcggaaaca gtccattaaa tagtt
//
LOCUS       DAZKCZ010000059        15407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000059 DAZKCZ010000000
VERSION     DAZKCZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.059"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..2213
                     /locus_tag="ACG8KN_001690"
     CDS             153..2213
                     /locus_tag="ACG8KN_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFP9188747.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2296..2865
                     /locus_tag="ACG8KN_001691"
     CDS             2296..2865
                     /locus_tag="ACG8KN_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9188748.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3040..3846)
                     /locus_tag="ACG8KN_001692"
     CDS             complement(3040..3846)
                     /locus_tag="ACG8KN_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188749.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3984..5498
                     /locus_tag="ACG8KN_001693"
     CDS             3984..5498
                     /locus_tag="ACG8KN_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFP9188750.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5901..6884)
                     /locus_tag="ACG8KN_001694"
     CDS             complement(5901..6884)
                     /locus_tag="ACG8KN_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFP9188751.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6889..7536)
                     /locus_tag="ACG8KN_001695"
     CDS             complement(6889..7536)
                     /locus_tag="ACG8KN_001695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9188752.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7621..8253)
                     /locus_tag="ACG8KN_001696"
     CDS             complement(7621..8253)
                     /locus_tag="ACG8KN_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9188753.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8269..8820)
                     /gene="coaC"
                     /locus_tag="ACG8KN_001697"
     CDS             complement(8269..8820)
                     /gene="coaC"
                     /locus_tag="ACG8KN_001697"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFP9188754.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8817..9554)
                     /locus_tag="ACG8KN_001698"
     CDS             complement(8817..9554)
                     /locus_tag="ACG8KN_001698"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFP9188755.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9551..10540)
                     /locus_tag="ACG8KN_001699"
     CDS             complement(9551..10540)
                     /locus_tag="ACG8KN_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9188756.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10798..11340
                     /locus_tag="ACG8KN_001700"
     CDS             10798..11340
                     /locus_tag="ACG8KN_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9188757.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11357..12442
                     /locus_tag="ACG8KN_001701"
     CDS             11357..12442
                     /locus_tag="ACG8KN_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188758.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12454..13260
                     /locus_tag="ACG8KN_001702"
     CDS             12454..13260
                     /locus_tag="ACG8KN_001702"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188759.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13261..14088
                     /locus_tag="ACG8KN_001703"
     CDS             13261..14088
                     /locus_tag="ACG8KN_001703"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188760.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14085..15158
                     /locus_tag="ACG8KN_001704"
     CDS             14085..15158
                     /locus_tag="ACG8KN_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9188761.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4953 a         2782 c         3138 g         4534 t
ORIGIN      
        1 aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact ctcctaatag
       61 tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt actacttcat
      121 gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac
      181 taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa cactacgtta
      241 agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg catgtacccg
      301 ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag accactgatt
      361 cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta cttcctgcat
      421 tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg ttaattccta
      481 acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc ttttccgata
      541 tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg aatcatgctt
      601 tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa aaagattatt
      661 cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg atatctgatc
      721 tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa
      781 acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc ttgtcctatt
      841 ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt cgtgctgatc
      901 aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac gcccctaaag
      961 atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa
     1021 tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta acttatatag
     1081 actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg
     1141 cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa cttcaagcag
     1201 aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct gaattttctg
     1261 atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc acttctattt
     1321 ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag atccttgaag
     1381 tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt acctttactt
     1441 atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt catccaaacg
     1501 aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt aaacccgtac
     1561 cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt tatttgacca
     1621 aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca ctccaagaac
     1681 tccatgctca gtcactatta catgttttac cattaaacaa acagatagat caaaaagaac
     1741 aaaaaagaca agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc
     1801 caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct agaaaaatct
     1861 cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt tctggattgg
     1921 aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt gttgctaaac
     1981 agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa
     2041 ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt ccgggaacac
     2101 ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga ggtatggcag
     2161 gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc tgatttatat
     2221 ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat aatggagacg
     2281 aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg gatgacaaga
     2341 ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt tcgattttat
     2401 caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga atttcaaacg
     2461 acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga agaacttgaa
     2521 agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg
     2581 cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt gaaaccagat
     2641 taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga atttcctacc
     2701 ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca ggcgattgct
     2761 ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac acctaatcca
     2821 gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa tggtgaaata
     2881 agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac ttttttgagt
     2941 taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg cagtgacaaa
     3001 agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga tttttttagg
     3061 aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga ggtaaccaaa
     3121 tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag tatccatgat
     3181 tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat ctgcattttg
     3241 actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc cgtctttact
     3301 taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga attgcagtaa
     3361 tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg aaaaacagaa
     3421 ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt tattgagcaa
     3481 ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat gtagaaaagt
     3541 atcccacatt gaaaaatagc cataaaaatc acttaactct cctaaaactg ttgaactaaa
     3601 aataaaagat aacaaaatga tttctagcat atttggaatc tgtatcttta atatcttttc
     3661 cagaaaataa gggatttcaa ataaaattaa tgtcagtaat aaaagcattg tatttcccca
     3721 gtgttggttc cagacctgat ccactagcat aataacgacg atgatacgta ataaaatata
     3781 gattccgttt gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg ttttcaaatg
     3841 attcataaaa taactccttc atgtatatcc ttaattatac taagaagcgt gtgtttacca
     3901 aagctatatg acttaaagaa aaacagagtt gatattgtga aaaaaatcac ttaatatagt
     3961 ataaggagaa aagggggata caaatgaaaa aattagttgc tggtttatta acagccgttg
     4021 cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag acaagttcta
     4081 gtacgaagga atctgccgaa gtatcatcag gcgcttctga acaagtatat acagatccaa
     4141 aagaaatgaa agaatcttat gatgtcgtga ttattggagc tggtggtgca gggatggcag
     4201 cagctatcga agcaaaagat gctgggttga atccagtaat tttggaaaag atgcctgttg
     4261 ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca aaatttcaaa
     4321 aagaagaagg aatcaaagac tcgaatgatg ccttctatga agaaacgcta aaaggtggtg
     4381 gaggaacgaa tgataaagaa atgcttcgat actttgtcga tcactctgct gatgcggtcg
     4441 attggctaga tcaaaacggt atcaaactgg acaacttaac gatcactgga gggatgagtg
     4501 aaaagaggac acatcgtccg tctgatggct cggctattgg tggatactta gtggaggggt
     4561 tattgaataa cgtccatgag cgggaaatcc ctatttttgt caacgcagat gtgacagaca
     4621 ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca tgttcaagga gaagaaccaa
     4681 aagaggtcac cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc agcaaagaat
     4741 ttattgaaaa atatcgtccc gatttagctg attatattac gacaaatcaa gaaggatcaa
     4801 ctggagatgg tatcaccatg attgaaaaat taggtggaca aaccgttgat atggataaaa
     4861 tacaaattca tccaactgtg cagcaggatg agggggtctt gattggtgag gttgttcgcg
     4921 gagaaggagc aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa atggatacaa
     4981 gggataaagt atctgcagca attacagcat tgcctgaaaa atcagcttat ttgatttttg
     5041 accaaggcgt gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa ggatatgtca
     5101 caactggaaa gacaatggaa gaattagcag aaaaaatcga tttaccaaaa gaaacactag
     5161 caaaaacagt tgatggatgg aatacggatg ttgcggagaa aaaagatgct caattcaacc
     5221 gtgccacagc catggaacat gacttatcgt ctccgaatta ttatgcgatc aaaatcgcac
     5281 caggaatcca ttacacgatg ggcggtgtga aaatcaatac gaacactgaa gtattagata
     5341 aagaaaacca gccaattagg gggttatatg ctgctggtga agtcacaggt ggcttacatg
     5401 gaaacaaccg gatcggtgga aattcagttg cggagatcat tatttttggg cgacaagcgg
     5461 gcatacaagc agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa ataaaaagag
     5521 gttgtgaaat cagcgttttg agctaatacc gagggtcagc tgattgaaca ctgtttagtg
     5581 aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct
     5641 tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt
     5701 attcggaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     5761 tataaacggc ggaagcagaa gaaattcctt cggaaataag ctgaaattca caaaaatttg
     5821 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     5881 cttttttgat cagcatgatt ttattcagtt atcttcaaaa tgatttttcc gatgtttttg
     5941 tttgcttcca tatattcatg cgcttgtttg actgcgctga attggaatgt acggtcaatg
     6001 attggccgaa tcttgccatt ttctagaaaa ggcccagctt tttgcataaa agtttgtgcg
     6061 agctctgctt tatactgatc acttctaggt gtcaataatg taccaagcag ttggacacgt
     6121 ttcccaatca atacaccaag gtctatctca ggcacgatgg tcccacctag cataccaatc
     6181 aagacccaac gtccatcaac ggctatagaa gctaaattct tttcccagta agaagcgccg
     6241 atgaaatcca aaataacatc gactccccga ccttcagtga tttttcgtac ctcttcttga
     6301 aagtcttgtt ttttatagtt gatcaattca tctgcaccaa gttctttaca aaaggcgagt
     6361 ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct agctaattgg
     6421 atcgctgctg ttcccacacc acttgctcct gcatggatca agacagtttc gccttcttgt
     6481 aattttccaa gccagtaaag tgtttgataa gcagtcaaaa aaacttctga gattccagca
     6541 gcagaaacat agtctagcgt atcagggaga aggattgcac gatcggcagg catcacagca
     6601 tattcggcat aaccacctag attgactaat ccgtatactt ttgtcccagg agtaaaagtg
     6661 gagatatcac ttttattctc aactacctct ccagctactt cgactcccaa aatagggtag
     6721 ggaggtttca aagagactga ttctcttttc ataatatctg ttcgattgac agctgctgta
     6781 tggactttga tcagtaattc gccttgtttt ggtttaggaa caggcacttc aatttgttct
     6841 aatacatcta cagaaccagg atgcttgatt gaaatggcac gcatggatct attcctcctt
     6901 tgcccattct tggaatgctg tattcaactc tttgctacct tcagaaagta ctagagtcgt
     6961 acgagaaata tccgtttctt ccactagagc ggcaagtaca gcagcaactg tagagcgagt
     7021 gatcgttttt gatgccttct cattaggctt agttaattga atggaagtaa cttcttcatc
     7081 gttagtcaat gtgactggtt gaagaatgac aaaatctaag ttagttcgtt tcagccattc
     7141 atctgcataa tgtttagtaa tataataatc tgtcatggac tcaggccatt ttgctggttc
     7201 atctgcataa acagcactga ccatcagata gcgagagaca tgcgcatttt cagctgcgat
     7261 cactgttttg attgcgccat ccaagtctac ttgcagaaga ttctttcctt gagagccggc
     7321 agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc attttttcca cggaccaagt
     7381 taaatcaaaa gagacatacg aaacattctc tttttttact aatgtttggg aagcaacatc
     7441 tctcacccct gcaaaaattt catgggaact tggagctaaa tcttttatca ggtgtcggcc
     7501 aatttgtccg tttgcaccta caacaaaaat tttcatttgg atcatccttt cgacgtcaaa
     7561 tctttccatt caatagtaac accaaaagag aaaaaaatca gagatcggct acgatgacta
     7621 tcagttagta agagaagggt gcttgaagta tcttaacaag acattgcata ctgctgcagt
     7681 cgcaagacct gcgacgattc cttcaggtat accacttgta aaaataaccg ctagaatagc
     7741 tgaataaaca gcatttaaat ttgttccgat gactttggca taatcttgct ggaataaaaa
     7801 gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc cagctaagaa
     7861 taaagaaacg gaatctgctt tttgtttcat tagtttgcgc agacctttat aaacgaaata
     7921 aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt tccagcttcc
     7981 atgttctgtt ccgatgactg ggataaacgg agaaaagaca aaagacaaag gtgtttggat
     8041 aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag cacctagtct
     8101 tggtcctaaa acgatcgaag caatgattac aggaatatgc atggttgttg cattgattgg
     8161 accgataggg ataaaaccta aaggcgtaac agctaataaa atcaaaatag ctaaaaacat
     8221 cgcagttaat gtaaagtttt ttgtattttt cataagtact ccttttttct aagcatttct
     8281 ttcattaaga gtcagcatga cctgttcgat gatcgtctga atatcagcta aggcaccttt
     8341 tccaaaatct ccacaagcta aaagagattc tcttggttca atttcctgat agccgacaga
     8401 tctcaatgtt tccagattac gctgagtgat cgggttttga tacatattcg tgttcatggc
     8461 aggggcaatc aattttggtg ttcccactgg aagcgccatt gcgactgttg aaagaagatc
     8521 gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg cgatgagaaa
     8581 caagtcagct tgttttgcta gttcgatatg attgattacc tgcggatttg attcttgcat
     8641 gacgtctgta tggactgcgc gcttcgacag agattgcaag gttaatggcg tgataaaatg
     8701 tgtgctgctt ttagacataa tcacatctac ctgggcacct aatttgacga attgacttgt
     8761 aagatcagcg cttttgtaag cagaaatact tccagtaacg cctagtaaga tctttttcat
     8821 tttgattcct ccagtctaat tttctcagta atcaatgcag caatctcaga tttactttga
     8881 gcttcttcca ctgttccatc tttagataag agatagccgt ggtgtgtctc gccatggatt
     8941 tccgtcaaat catttgcgaa aataaaatca gttttgttat ttatcagact attttttgcg
     9001 actgccagaa gagattcctt tggtacatct actaatagtt tgaaaccaat gatttttgct
     9061 tttggctgcc attcacgtag ataagaaatg atttttggtg ttttcttcaa aacgagcaca
     9121 agatgctcgg tgttagaaga gatctttgaa acagtggtat ctgtctgttc attttccgca
     9181 aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc cagctgttct
     9241 tctgaaaaac taaaagcagg agtaaaatca ctgactgcca tactgtggat aatcgcatcg
     9301 tagtgttctt ttttcgttag ttcttctaat tgtaaaaata agtccagtgc agattcgatc
     9361 gtataaatcg ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt agtcacgtaa
     9421 tcgattgtgg tatccggatt ggcagctaaa taatcagcta atgcttgccc taatcgtcct
     9481 gtcgaatgat ttgtaatagc acgtacttga tcgatttttt cagtcgttcc tccagctgtg
     9541 attagaatct tcatttatgg tcacctacat ttactagtgt tcttccttgg tgtgttccag
     9601 caagtagttt agatgctgtt tcaggcaatt gatccagagt aatctcttgg acgaccagtt
     9661 catctgcaat ctgcaaatcc gccatctgtt gccataaaga aagacgttga tctattggta
     9721 cattgacaga atcaattcca atcaactgga tgtttcgcag aataaaaggc aagacagtcg
     9781 cgtttagttt gattccccca gcattgccac ataaagctac tgcaccgtta taagagatca
     9841 atggaagaag actgcttagc tgctcgccac ctactgtgtc gatgacatag tcgatttgct
     9901 gttttcctaa agcttttgtt gtctctggca gaaattcatc aggtcgtacg acttgcaaag
     9961 cacctagact ttttagccat gtgacagact cttcttttct ggaaaaggca atgattgact
    10021 ggaatcctag tttatgtaac agggctatcg ctgtactgcc tacaccgcca gaagcaccag
    10081 taacgacaac ttttttatcg gaggtcatac cttgacggat taaagcgttc acacacaaca
    10141 tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg cagcgtcata ttttttggta
    10201 gaggaactaa ccagtctcct ggtacttttt gatattggct ataaccgcct ggatgggtga
    10261 cgccaagccc aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt ggattgttac
    10321 tttggacgac agttccggat aaatcaatcc ctggagtcat cggatatgtt cgtatcacct
    10381 gaccagactc gctcattgcc aatgcatctt tataattgac atcagaatag gcaattttta
    10441 ccaagacttc attttcagcc aactcagtaa atggcagatc actgattgct gtatggaaaa
    10501 caggttcctt tattacctga aaagatcgaa aagcgttcat tcgtgagaca ctccttttaa
    10561 aattttcttt tatcatacca taaagcctac tcttttattc ttgttcttcc gtaaatttag
    10621 acaaaaatta ggtgaaaaat aaagaatcaa acaactagaa tttattccaa aagataaaga
    10681 aatattcagc aaaaaaaatt ttacaaaggg ttgccttttt caataaattt cgtatactaa
    10741 tacgagtggt agtaaacagg aagtttacta ttagaaaact tcgattcgga ggaaattatg
    10801 gaaattggtg agaagttaag aaatttgcgc atccaaaaga atttgacaca agaagaacta
    10861 ggcgaacgca cagatttgtc aaaagggtat atttcccagt tggaacgaga tttaagttca
    10921 ccatcaatgg aaacattctt ttctattctt gaagtcttag gtgtgactcc agaagaattt
    10981 tttcatcaag aagcagcgaa tctgcaagtg gtttattcaa aagaagatca tacggtttat
    11041 ttcgatgaag aaaacgggta tgaattggaa tggctcgtgg ctgattcgaa tgaaaaagaa
    11101 atggagcctg tcttgctgac ttttgaaggt gcaggagaat ataagacgtt tgaaccttca
    11161 ttatctgaaa cgtttatttt tgtattagaa ggggaattag agttaagctt aggagagacg
    11221 gtctatgtgg caaaagcagg acagtcgatc tattatcaag cgacaaaaca acatcaatta
    11281 aggaatcatc tcaaaaaacg aacaaaagta ttgattgttg caacgcaatc atatttgtaa
    11341 tatagggggt aaaaacgtgg ggaaaccaat catttcattt gatcaagtag tgaaacgcta
    11401 tgatgatgaa acagtattaa aaaaagtaag ttttgagatc gaacaaggaa aattttatac
    11461 actgcttggt ccttccggct gtggtaaaac aacgatccta cgtattatag ctggattcac
    11521 cgaagctaca gaaggggaca tctattttga agggaaacgg ttgaatgatt tgccagctaa
    11581 taagcgacaa gtaaatacag tcttccaaga ttatgcactt tttcctcata tgaatgtttt
    11641 tgataacgtt gcttttggat tgaagatcaa gaaaatgtcc aaatcagata tcgaaaagaa
    11701 ggtaaaagat gctttgcgta tggtccagct tcctggatac gaaaaacggg agattagtga
    11761 aatgtctggt ggtcaaagac agcgtgtagc gattgcacga gcaattgtaa atgaaccgaa
    11821 agtattgcta ttagatgaac cgttatcggc gcttgatttg aaactacgta cagatatgca
    11881 gtatgaactg cgggatctgc aacaacgatt aggaatcacg tttatttttg ttactcatga
    11941 ccaagaagaa gcgttggcaa tgagtgatga gatttttgtc atgaataaag ggaaaatcgt
    12001 ccaaagtggt acacccgtag atatttatga tgagccaatc aatcatttcg ttgctgattt
    12061 tgttggggag agcaatatcg tggacggtgt aatgatcgaa gataatctag ttgagtttgt
    12121 tggtaagcaa tttgaatgtg tcgatggcgg gatgcgtcta aatgaaccag ttgaagttgt
    12181 tttacgccca gaggatttga cgatcacgac accagataaa ggaaaactag tcgtaacggt
    12241 cgatactcag ctgtttcgtg gtgtccatta tgaaatcatc tgttatgatg aacagcaaaa
    12301 tgaatggatg gtccattcaa cgaaaaaagc taaagaagga agcaaagtag gattggcctt
    12361 tgagccagaa gatatccatg tcatgcgttt caacgagtct gaagaagaat tcgatgcacg
    12421 tcttgacagc tacgaagaat aggggggaca agaatgaaaa caatgcgcag gatctattcc
    12481 gtcccttata ttttgtggct cggtttattc gtgatcgcgc ctgtattaat gatcatttat
    12541 caatcttttt ttgatatgaa tgggcaattc acgctgaata attatctatc ttattttacg
    12601 tcggggacat cgctttctat gacgatcaat tctgtatggt atgcattttt gatcacgttt
    12661 ttcacgctga tcatcagtta cccgactgct tattttttaa caaaactgaa gcacaagcaa
    12721 ttatggctga tgctgattat tttgcctaca tgggtgaatc tgctgcttaa agcttacgca
    12781 tttattggga ttttcagtgt gcacggcagc gtgaatcatt tctttgattt tatcgggatc
    12841 gggccgaaac agatattatt cacagacttc agttttctgt tcgtggcaac gtatatcgag
    12901 attccattta tgattatgcc aattttcaat gcattggaag agatgaatcc ttctttgatc
    12961 agtgctagta gagatttagg cgcgaatagc tttgagacct tccgacgagt gatttttccg
    13021 ttatcattaa atggtgtgaa aagtggtgtc caagcagtat ttattccttc tctttcttta
    13081 ttcatgctga ctcgtctgat tggcggaaat cgggtgatca ctttaggaac ggctatcgaa
    13141 cagcatttct tggttacaca aaactggggg atgggttcta caatcggtgt gattttaatc
    13201 attgctatgt tcatcgtgat gctcctgaca ggcgagaaga aaaaaggagg tcgctcataa
    13261 atgaaaaaat ttaaatggtc gtatctttat ttagggatcg tctttttatt cttatatgca
    13321 ccgatcttct atctgatttt ttattccttc aatgaaggag gaacgatgaa cagctttaca
    13381 ggatttactt gggaaaatta tgaagctgtt tttgaagata cacgtttgat cattatcgta
    13441 ttgaatacgt tcatgctggc tttcttatct gcattgatcg ccacgatcat cgggacattt
    13501 ggggctatgg gaatctatta cacgaaaaaa cgacgtgcac ggacagcgtt gttgagcatg
    13561 aataacatct tattagtctc tcctgatgtt attatcggtg ctagtttctt gattttcttt
    13621 acactactag gattcggatt aggttttcag agtgtattgc tttcccatat tgctttcagc
    13681 attccaatcg tcgtcttgat ggtcttgcca aaattgcagg aaatgaacga ttcaatggtc
    13741 gatgcggcta gagaccttgg tgctaataat ttccaagtag tcaaaaatat cattttgcct
    13801 tatctttctc caggaatcat agctggatat tttatggcat ttacctattc tcttgatgat
    13861 tttgcggtta catttttcgt cacaggaaat ggctttacga cgctctcagt agagatctat
    13921 tctcgtgcaa gacaaggaat cagtttagaa atcaatgcgc taagtgcgtt agttttcttg
    13981 ttctccatga tattggtcat tggatactac tttattagta aagaaaaccg tccaaaacgg
    14041 ctgaagaaaa acagaagagt acgcgagggg gtggcacggc tgcgatgaaa aaattacaat
    14101 ctctgttttt aggtattctc atcattattg cggtgctgct gcttggtgtt cgtcaacttg
    14161 aaaaaacaag tggcatgagt ggagcaaagg tacttaccat ctataactgg ggagattata
    14221 tcgatcctag tttgatcacg aaatttgaaa aagaatatgg atacaaagtg aattatgaaa
    14281 catttgattc taatgaagcg atgttcacaa aaatccagca aggtgggaca aactacgaca
    14341 tcgctatacc aagtgaatat atgatccaaa aaatgatcaa agaaaaacta gtacttccat
    14401 tagatcattc gaagatcaaa ggattaaata atattgatcc acgattttta gacttggatt
    14461 ttgatccaga taatcagtat tcgattccat atttttgggg aactttaggg atcgtatata
    14521 acgataaatt ctttgatgca aatcaaatta agcattggga tgatttgtgg aagccggaat
    14581 taaaagacag tttgatgctg attgacggtg cacgagaagt tatggggctt tctttgaata
    14641 gtttaggcta ttctttgaat agtaagaata tggcacagct gacagaagca gcaaataaat
    14701 tgaatcattt aacaccaaat gtcaaagcaa tcgtggcaga tgagatcaaa atgtatatga
    14761 tcaatgaaga agcctccgtg gcagtgacat tttctggcga agctgccgat atgatgtggg
    14821 aaaatgaaca tttacattat gtgatcccac cagaaggatc caatctttgg tttgataata
    14881 tcgttatgcc aaaaaccgtg aaaaataaag aaggtgccta tgatttcatc aactttatgc
    14941 tagaaccgga aaatgcggca caaaatgcag agtatattgg ttattctaca ccaaatgaag
    15001 aagcaaaagc gttattgcca aaagacattt cgtcggacga acaattctat ccatctgatg
    15061 acacgatctc ccatcttgaa gtctacaaag atctaggatc gaaataccta ggcatctata
    15121 atgacctatt cttagaattc aagatgtata ggagataatg gcaaaactca gtaagaatgg
    15181 ggacttatca acgttctaaa gagctaaaaa tcgtcctttt tctatctgag ttttccaatc
    15241 ttctaccaaa agcggatgaa acgccgcttt ttttgtgtta ttttattttt tctaccacgt
    15301 ttttctacca gagaaaagtg gacaaaacca ttgtaaaata gggcccgaga cgggctagta
    15361 aatctaagat tgaaaaagtt acagaaggat aaagtaattg gaaacta
//
LOCUS       DAZKCZ010000060        15074 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000060 DAZKCZ010000000
VERSION     DAZKCZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.060"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACG8KN_001705"
     CDS             complement(<1..1128)
                     /locus_tag="ACG8KN_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9188762.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYF"
     gene            complement(1282..1812)
                     /locus_tag="ACG8KN_001706"
     CDS             complement(1282..1812)
                     /locus_tag="ACG8KN_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9188763.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(1911..2261)
                     /locus_tag="ACG8KN_001707"
     CDS             complement(1911..2261)
                     /locus_tag="ACG8KN_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFP9188764.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(2418..3947)
                     /gene="ahpF"
                     /locus_tag="ACG8KN_001708"
     CDS             complement(2418..3947)
                     /gene="ahpF"
                     /locus_tag="ACG8KN_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9188765.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(3957..4520)
                     /gene="ahpC"
                     /locus_tag="ACG8KN_001709"
     CDS             complement(3957..4520)
                     /gene="ahpC"
                     /locus_tag="ACG8KN_001709"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9188766.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(4664..5315)
                     /locus_tag="ACG8KN_001710"
                     /pseudo
     CDS             complement(4664..5315)
                     /locus_tag="ACG8KN_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            5499..7145
                     /locus_tag="ACG8KN_001711"
     CDS             5499..7145
                     /locus_tag="ACG8KN_001711"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9188767.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            7136..8218
                     /gene="uxuA"
                     /locus_tag="ACG8KN_001712"
     CDS             7136..8218
                     /gene="uxuA"
                     /locus_tag="ACG8KN_001712"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9188768.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(8326..9879)
                     /locus_tag="ACG8KN_001713"
     CDS             complement(8326..9879)
                     /locus_tag="ACG8KN_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9188769.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            10027..11196
                     /locus_tag="ACG8KN_001714"
     CDS             10027..11196
                     /locus_tag="ACG8KN_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9188770.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(11322..12401)
                     /locus_tag="ACG8KN_001715"
     CDS             complement(11322..12401)
                     /locus_tag="ACG8KN_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9188771.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(12510..12950)
                     /locus_tag="ACG8KN_001716"
     CDS             complement(12510..12950)
                     /locus_tag="ACG8KN_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9188772.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(13007..13417)
                     /locus_tag="ACG8KN_001717"
     CDS             complement(13007..13417)
                     /locus_tag="ACG8KN_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9188773.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(13598..14032)
                     /locus_tag="ACG8KN_001718"
     CDS             complement(13598..14032)
                     /locus_tag="ACG8KN_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9188774.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(14132..14980)
                     /locus_tag="ACG8KN_001719"
     CDS             complement(14132..14980)
                     /locus_tag="ACG8KN_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9188775.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         5153 a         2827 c         2614 g         4480 t
ORIGIN      
        1 aaaatatttg agaacaagtg gagaaataag tgtacttaat aaaatcacga taactaaagg
       61 tgaatagtac tgtggttcga tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc
      121 catttctcca cgcgaaatca taccagcacc gaccatcaat gaactatgtt gagaaaaacc
      181 ggcaatctga gcaccaaaat aacctccaag caatttggtc aaaatcgcta caagcgtcaa
      241 tatagaaata aagagtagtt gttcactaaa gtcagaaaaa tcaacttcta atcctacact
      301 gacaaaaaat actggtataa atactgcata tcctaaagcc tctacattgt ggtatacttc
      361 ttctttaacc tttgtctgac tgacagctat cccagcaaaa aaagcaccaa tgacagagct
      421 aagcccaaca agatccgcta cgtaagacat tcctaaacaa ataactagcg atacgatgat
      481 aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt aggaaaggcg caatccattt
      541 gactaataag aaaataaaaa caaaataaat tagctgttca atcaaaacaa gcggtaaaga
      601 agcattgcta gaagcttccc ccgttagaaa agataggctg aaacttagaa ctaaaacgac
      661 taaaatgtca tctaccacag aagcacccaa aattgttgat ccttcttttg tattcactac
      721 gtttagttct tttaatactt ctactgaaat actgacagaa gttgccgcaa gaataatccc
      781 aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac
      841 aaccgggaag atgatcccaa gcaaagcaac gaacatacca ggtcggaaat attttttcaa
      901 aagagataaa tcactttcca gaccagctaa aaacattaat aagatcacac caatctccga
      961 aaagtcatgg actagaatat caggtttgac ccaattcaat ccagcttggc ctagcaaaac
     1021 accaacaagt aattgtccaa tcactgctgg gatacctaat ctccgcgaga tatgtgagcc
     1081 aatcgttgtt gaaatcaata tcaggcataa aataccaata aattccattc tcatcattcc
     1141 tttgtataaa atatagcccg aacttagcat aacatacatt attgaaaaaa taggaaaata
     1201 tactcacaaa aaaaccaaca atccaaaaga tttttttgga ttgttggttt ttatacggtc
     1261 tattttaata actaaaaatg attaacgtat agtcattttt cttattctga tgaaattgat
     1321 aatctcctgg atgcccatga aaatcaaaat aaagccgaat atttggaata gaagcatcaa
     1381 acttttgaat ggattgaaca ataagacgaa tccgccaatc aacaacagaa tactactata
     1441 aacactccaa cttttagctt ttgtattcat gctgaagaac aactggaaca agctgttgat
     1501 gataacagct aatccaagaa gaatcggcag gatagaaacg attgcaggtg caaaagcata
     1561 gacgatcaga gctaagatca ggaaaaaaag accactgaac agaccgatac tccaagaacc
     1621 tgttttcctt ttaaaacggt aagcctcaaa aagattcaac aacccaagca aaacaagata
     1681 ggcggtgatc agataaccga taaaatgaaa aacagcagaa gggtttagca caatcgcaat
     1741 cccagaaata atataaacag cagctcttaa taaggcgtat ttttgaatat tttgtttaat
     1801 cgtctcaaac atataagaaa acctcctttg acttatttta acgtgaacta gtcattttac
     1861 caaattaaat gttacaaaat gagcgaagga gaagaatatc tctggaaaaa tcaacttctg
     1921 aggatctttt ccattttttt gccttttgct agctcatcga tcattttatc taaataacga
     1981 atttctttca tcaagttatc ctcgatgtct tctactcgta caccacaaac aacacctgta
     2041 atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa aaaattgttc gaaagtcact
     2101 tgtttttgta gttgatattc tagttctgtt tgggaataac ccgtcagcca acaaatgatt
     2161 tcatctactt cctcttttat acgatctttt ctctccgctt ttgctacata cataggataa
     2221 acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca taatcagtca cctttctttt
     2281 tttcaaatta tttactactt atagtatata agaaaaagag aagaaaaagc gattattcaa
     2341 aaaaacaaaa aggattgtga caaaattctt gtcaccatcc ttttttccgc tctctgactt
     2401 tttacaatct tcatagctca agtgcgcatc agataatcga atgctcctaa agctgcagta
     2461 gctcctgaac ccattgaaac aatgatttgt ttgtatggac tatctgtaca atcaccagct
     2521 gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg tcactatttc atttcttgga
     2581 ttcatttcga tcgttccatc tagccaatcc gtattaggga gtaagccgac tagaatgaat
     2641 actcctgaaa cagctagtgt atgcatctcc atcgtacggc gatcgatata ggagagagat
     2701 tctacctgat tcgtaccgct gatttctttc gtcgctgcat gtttgataac agtgacattc
     2761 gcaagagaga ctagtttatc ctgtaagatt tgatctgctt ttaattctgg taagaattct
     2821 agaacatata catgttttgc taacccagcc aaatcgattg ctgcttcaac accagaatta
     2881 ccaccaccga taacagcaat ctcttttcct ttaaataaag ggccgtcaca gtgtgggcaa
     2941 taagcaattc ctttattttt aaactctttt tcgcctggta cattgataga acgccagtgc
     3001 gcacctgtcg cgatgatggc ggtttttgct ttaagtgata taccgttagc taagccaata
     3061 ttaataaaat gttgctcttt atttaaggaa acggcctgtt gattcatcat aatgtcgata
     3121 gggtaactac gcacatgact ttctacttgc tgcatcaatt ttggaccttc tgtgtaagag
     3181 gtaccaatca tattttcaat ccctagtgtt tcaacgactt gtccgccgaa agaatcagcg
     3241 acaagtcctg tacggattcc tttacgtgct gcatagatag ctgcactact agctgctggt
     3301 ccaccgccaa tcactaatac atcataaggt tctttttcaa acaactcttc ttcaatcaaa
     3361 gggccagtga ttttttcaac aatttgttcg atcgtcatgc gtccgctact aaattcttct
     3421 ccatttaaaa acacagttgg cactgccatg atttttttat catcgatttc tttttggaac
     3481 atcccacctt caatcatcgt atgagtaata tttggattta atacagctaa tatattcaat
     3541 gcctgtacaa cgtcggggca attatgacaa gtcaagctaa cataactgtc aaaatgcaat
     3601 gtttgtgtta tttttttgat tttaagactc aaagattcac tgattttcgg tgcccgtcca
     3661 ctaatttgta acatagccag taaaaaagag gaaaattcat gaccgagggg gataccagca
     3721 aaaacgattc cactttcttt gccaactgga ttgatagaaa aactaggtgt gcgcatcgaa
     3781 tctctttttt cccaagagat tttaggagac atttctgcta tttccttcgt aaattcagct
     3841 agtttttgac tattttcact attatccaaa ctaacagaaa aaacaatagg agattcaatc
     3901 agtgctaaat attgttcgag attttgttta gtttctttgt ctaacatatc caccacctaa
     3961 atctttccaa caagatcaaa acttggtttc aatgtttcgc cactttcttt ccattttgcc
     4021 ggacaaactt cacctgggtg ggtacgtaca tattgagcag cgcgaatctt atcaatcagt
     4081 gcactagcgt cacgaccgat tccatctgca ttgatttcca tagcttgaac gattccgtct
     4141 ggatcaataa taaatgttcc tcgctgagct aatccttgct cttcatccaa aacatcaaaa
     4201 atacgagaaa tctgatgaga cggatcaccg atcatcacgt attcgatttt accgatagca
     4261 tctgatgtat catgccatgc tttgtgagta aagtgggtat cggtagagca agaaaagact
     4321 tcaacaccta aagatttcaa tgtactgtat tgttcttgta agtcttctaa ttcagtagga
     4381 caaacaaaag taaaatcagc tggatagaag caaagaatac tccagttccc cataagatct
     4441 ttttcactga cttctaaaaa ttctccttga tgataagcat gtgcattaaa ttcttcgatt
     4501 tttttcccaa tcaatgacat agtaaaacct ccaataataa ttatttattt ataattattg
     4561 taatttagtt ggagttgcaa agtcaatgga tattttgtta tttaataaag attactcaaa
     4621 aacgaaggtt aattataaaa atagaagagg tcgcaagaaa atgctactgc tggaaatact
     4681 cgggataacg ttgagtaaca gtttcctgtt ctttcagcat catgtgtaag tgcaatcccg
     4741 tttgaaatcg agcttcttct ggttttttat caactaaagc cttgagtaaa tcataatgtt
     4801 gttccatgat cgtttcccat ggaagcccct gaatggtcac tcgtaaccag cggaaacgtt
     4861 ctaaatgggt attatggtca tctaaccagt tccaaacttc ttttcttcct gcaatttcga
     4921 aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc gcgttcgtca tttattttga
     4981 ctgctttttc ttgttcttct aaaatcgttt ctagcactat tcgtgtttgt gtgtccaatt
     5041 tggaacaaca ttcaatcatg atttgctctt ccaactgttc tcggacaaag cgagcatttt
     5101 ctgcagaaac aagattaatt tttgaaacat acgtaccact ttgaggtatc gtatacacga
     5161 gttcttgctg acgtagttga atcaatgctt cgcgaatggg agtccggccg atagcaagat
     5221 gttcctccag tgccttttca gatattttcc ttcccagcat agataatttg tttacgaatc
     5281 gcttgataag cttgagattg caagctgctt ttcatcttta ttcctccaaa atcatttcta
     5341 tgaacgtagt ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa
     5401 atttgtataa atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt
     5461 gaatacaaaa actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga
     5521 ggaactttat ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat
     5581 cattaagaga aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt
     5641 atcgaggatt tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg
     5701 gtgtcattgt ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg
     5761 atgatttgat tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag
     5821 ctgcgacagc tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga
     5881 ttcgacactt tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt
     5941 atcgattgaa agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg
     6001 gtccaaagaa gccatcacat acaatgagta ttcttgctgc gctactttat aaacgttttt
     6061 tatctggagc ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc
     6121 aattacgtac ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa
     6181 atacatttat ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg
     6241 atcgtatcac cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt
     6301 caaatatgtc gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag
     6361 aggacatcca ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa
     6421 aagctggagt attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga
     6481 cgacttgcct taatcctttg catacgtctt tagctattat gggttgttta ctggggtata
     6541 ggagcatagc tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt
     6601 acaatgaagg cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg
     6661 aagaagtaat tactgtgcgc ttttctaatc caaatattcc tgatatgcct cagcgaatcg
     6721 ccacggatac ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg
     6781 aaatagaagt taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc
     6841 ttcgatacct tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac
     6901 tattgacaga ctttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac
     6961 atattcatga gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt
     7021 accaagcaga gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccga
     7081 atgccgtacg aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga
     7141 aatgactttt agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca
     7201 gattcctgcg gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg
     7261 gcctagggag aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa
     7321 agtaattgaa agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca
     7381 gtatatcgaa aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat
     7441 ctgctataat tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga
     7501 agacggctca tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat
     7561 cgtcgcgcgc atcgaacatt ccagcaatga atttgaattg ccgggatggg aacctgagcg
     7621 cttagctcat atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg
     7681 agaaaatttt gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa
     7741 aatggctgta catccagatg atccgccata ctcgattttt ggattacctc gaatcgtaaa
     7801 gaatcgagaa gatttagatt ggttgtgccg catagtagac agcccttata acggaatcac
     7861 tctttgtaca gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga
     7921 attttgtcaa agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa
     7981 tcgagatttc tatgaggcac cgcataaatc cgaatacggt tcattagaca tgtaccgcat
     8041 actaaaaaca atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat
     8101 gatctggaat gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt
     8161 ttatctaaat ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg
     8221 aaaaaaactg taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat
     8281 ccggaggttg ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg
     8341 attttctttt ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga
     8401 gatcctcata atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat
     8461 ttttttgata tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt
     8521 ctccataaaa tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac
     8581 gatcagatct ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg
     8641 ctgttttttg tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc
     8701 cttttctaaa ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg
     8761 ttcaatggcg gtttggactt ctttcaacac gctatgttct tgataaagca tgaaagtagg
     8821 aaggggcgta tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa
     8881 tgccaatgag taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaaa
     8941 ggactccacc atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt
     9001 gtattgctcc aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat
     9061 attcactaag atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga
     9121 gggatcaata aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac
     9181 agaagttgca ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat
     9241 accgctattt agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac
     9301 tacttgcgga tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat
     9361 atctaatgct gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt
     9421 agaaaaggag atagacaaac cgtacaatgc taaaaatgca ttaatctttt tcattttcgt
     9481 atacaaataa gaggcactat agtttgtttg ctgacttaaa ctgataatcg aatttgtctg
     9541 acagctgaac aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa
     9601 tagacgataa tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt
     9661 ttgcttatca atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt
     9721 gatgtatagt aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt
     9781 aataaagaaa cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt
     9841 cttcaagaga aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt
     9901 aaattgttaa aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta
     9961 aacatatagg tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag
    10021 agagttatga gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt
    10081 actataaatc agacagtcca cacagaggag atagaaacag ccaaatggac agaaaccaag
    10141 tgggccaacg tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc
    10201 gcaaaattaa gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcaggagac
    10261 ttagcaccta tactacaatc agctgttaca tttgagaatg ttgatcccgc tttgtcgatt
    10321 ggctatgacg agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt
    10381 acattaaatc aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta
    10441 ccgaacgatc atcatcattt aacgccgtat agtcagcggc agacaattga gcaagattca
    10501 gctatcgact caggaggaga tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat
    10561 aagccagaag taccgcctac agaaaatcca gacggagaac agaaaccgga aatagaacct
    10621 ggagaagaac ccgatacaga aactcaacca gagccagaca atgaatcaaa acctgaaata
    10681 acccctggag aaaaaccaga tgtagaccca gaagaaaaac cggatgtgac tccagaacca
    10741 gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact
    10801 gaaaatccag aaaaactaga ggtagatcca gaagaaaaac cagatgtgac tccagaacca
    10861 gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac aattcaagag
    10921 gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa tgaagagcag
    10981 ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt aaaaagtagc
    11041 aaggatacaa cctttgctag tcctgaaacg aaaaataaac agctaccaaa atccggagag
    11101 tcacaaaaca aagttatact gtggagcgga atcattctat taagtatagc aacaatgctt
    11161 tctgcaaaaa gatttaaaca aaacagatct ctctaacatt tgaataatgt ctgtgaacgc
    11221 atttttcaaa taaaaagaaa tatccagaaa acgaccccaa aataccagat tgaaatctga
    11281 tatttggggt cgttcatact ttatctttct tcatatttat tttaagggat atctgtttct
    11341 tttggaagat ggttcgggat ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc
    11401 ggaatgccga caatcaagcc ccaaacgcca aagaaccgtt cgctaacaag taagaccaca
    11461 aatgtataaa aaatcggcag ttctgttttg ctggacatca acttcggatt cagcacataa
    11521 gactcaatta gatggacaat caaaatcgta atcaggatat aaacaacatc tcggatgcct
    11581 ccttgggaat aagctatgaa agatagagga atacaagaaa tgataacacc tgctactggg
    11641 atcagactta aaatgaaaat catgatcgct aaactcagca gctgatgaaa gccaaataca
    11701 gccaaggcga ttgtagtgag caacgtgttt aaaagagcga taacaaattg agcttctaat
    11761 accaaaccaa atgtattgac aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa
    11821 gaaaattcac tttttaaaaa caatcttgag aatacaattg tttttttctt ttcaatcata
    11881 aaaaagaaac ttaagatgaa tgacatagca aacgccactg ccatttttcc tatatcctgt
    11941 aaatagccaa gcgcaatcga agcgccattt tgaagctgtt caaaaaaatc gtttcgctca
    12001 atataagtat ggatatattg taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa
    12061 tcaataacgg actggatcat atcaaatgtt tgatgaataa gcatcgggat atactttgtg
    12121 aatgccagat agataaggta aacgatcagt gtataaatca tgatagctaa aacacctgta
    12181 gggattcgga caaatcgctg aatgatttga acaagatgaa tcatcaaata agtaaagata
    12241 aatgtgagca aaatggttgt aatcatactt ctaactaaga aaagaacaaa aacgacaaat
    12301 aataaaacag tgagacggcg tattttttca ttcgccaaaa aacgctgata aacggtcaaa
    12361 tcgacaacct ccaaataaca aaaaataaac acgttattca ttatatgatg gaatatgaaa
    12421 ataagaaaga gaaagagcaa aaagaaaaac cgctaaatga cgttatctta tgagttggtt
    12481 ttcctcataa aatcgttcat ctagcagttt cagccctttt tctggtcttg atgaaaatta
    12541 tctagcattt ttcgggtcag atgttgcagt gtatccaatt cttcctgttc aagtggaaga
    12601 taagacttga tcttttgtgg aataccttct gcttgttttt gcaacgcagc accttgagga
    12661 gtcagcgtaa catttacgat tctttcatca cccgtttcac gctgacgatt aatataattt
    12721 ttttctgcta acttttttag taagggagtc agcgtaccag aatccagaca caaatcattt
    12781 cctagctctt tcactgaagt ctttttcttt tcccataaaa ctaacatcac aagatactgg
    12841 gtataggtaa gattcagcgg cttgaggagg gaagtgtagt gtctaatgat ttccttagag
    12901 gcggcatata aaggaaaaca tacttgatta actaaatata gatcttccat ttttccacct
    12961 ccgctgtttt actatagtgt atcatagcgg atttacacaa aaaagtctat tcactaactg
    13021 ctttcagagt aacttctata tttccagctg tagcttttga atacggacac actttatgcg
    13081 ctgcctctaa taattcttgc gtttcttcca gagaaagacc ttcgatagat ccttcaatct
    13141 ctacacctaa gacaaagctc gtttcacttt ctgaataaag ggaaacttgt gcactgacag
    13201 tagaagcccc agagatattt ttttgctgtt tgatcaattc taatgcactg ttaaaacatg
    13261 cactgaaacc agctgcaaac aattgttcag ggttggttgc attttcgacg cgtttaccag
    13321 gagctgcaat attcaattcg aatgtacgat cgggattatg aacttctcca gagcgtccgc
    13381 ctgtgttgat cattttcgta gaatacattt ttttcataaa attaccacct ttaattatat
    13441 tttatacaat tgaattttac acaatataat tgtggaaagc aaagaaatgg attggcataa
    13501 gataaataag agaaagcaca tgaaacagac gcgctttctc gaatagcaaa catatacaaa
    13561 aaaatatcag agacaaaaac taatctcatc ataaacatta catatatttc tcaaaaagtt
    13621 gttttctttc ttcaaaactt acttcacgat ggacctgatg aagcatccat aaataaccaa
    13681 aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc atttcttgaa
    13741 tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca gagacgatga
    13801 tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca agagacgtgt
    13861 tttcatccag catatggaat ctcgtgccat acaaggtaaa aatgacttca ttattgcctc
    13921 tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga tacagttcta
    13981 gtgctttcaa actatcattt aatacaaaat ctatttccgt tcctttatgc ataaagatct
    14041 cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagtta aagaaaacgc
    14101 attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa gtaaatccgc
    14161 gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg atttcttcta
    14221 tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa acctcttttg
    14281 actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca atctcagcac
    14341 ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca gatttctttc
    14401 gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata gataaaatag
    14461 cataaagaaa ctcttctatt ccaaaaacaa acagattaag agaaacaacg aatgcgtctg
    14521 taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg aggggaggga
    14581 tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct aaagcaaaaa
    14641 tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt tctggaacta
    14701 ttgcaatgat taaaggaaaa aacaagcttc cgaaaagtac tttaaaaaag ggttttttac
    14761 ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata actatagctc
    14821 ggtcaaatcc taaaacagct tccagtaata ttcctagacc gctgacacct ccagcagcaa
    14881 tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg gcaaaaataa
    14941 tcttaatgta tgtccatata aattgtctcg tttctttcat ttatccactt ccttaattca
    15001 ttaataatac tcttatctta ttataactta cttttcacct taagttaact ttaattaata
    15061 aaaaaattag gcta
//
LOCUS       DAZKCZ010000061        14889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000061 DAZKCZ010000000
VERSION     DAZKCZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.061"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1897
                     /gene="yfcC"
                     /locus_tag="ACG8KN_001720"
     CDS             158..1897
                     /gene="yfcC"
                     /locus_tag="ACG8KN_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9188776.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2054..3238
                     /gene="iadA"
                     /locus_tag="ACG8KN_001721"
     CDS             2054..3238
                     /gene="iadA"
                     /locus_tag="ACG8KN_001721"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9188777.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3349..3552
                     /locus_tag="ACG8KN_001722"
     CDS             3349..3552
                     /locus_tag="ACG8KN_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9188778.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3653..4315)
                     /locus_tag="ACG8KN_001723"
     CDS             complement(3653..4315)
                     /locus_tag="ACG8KN_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188779.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4328..5830)
                     /locus_tag="ACG8KN_001724"
     CDS             complement(4328..5830)
                     /locus_tag="ACG8KN_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188780.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6378..8057
                     /locus_tag="ACG8KN_001725"
     CDS             6378..8057
                     /locus_tag="ACG8KN_001725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9188781.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8184..8663
                     /locus_tag="ACG8KN_001726"
     CDS             8184..8663
                     /locus_tag="ACG8KN_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFP9188782.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8882..9562
                     /locus_tag="ACG8KN_001727"
     CDS             8882..9562
                     /locus_tag="ACG8KN_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9188783.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9570..10901
                     /locus_tag="ACG8KN_001728"
     CDS             9570..10901
                     /locus_tag="ACG8KN_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFP9188784.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10916..11878
                     /locus_tag="ACG8KN_001729"
     CDS             10916..11878
                     /locus_tag="ACG8KN_001729"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFP9188785.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11989..12327
                     /locus_tag="ACG8KN_001730"
     CDS             11989..12327
                     /locus_tag="ACG8KN_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9188786.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12406..13113
                     /locus_tag="ACG8KN_001731"
     CDS             12406..13113
                     /locus_tag="ACG8KN_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFP9188787.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13216..14697)
                     /locus_tag="ACG8KN_001732"
     CDS             complement(13216..14697)
                     /locus_tag="ACG8KN_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9188788.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4729 a         2555 c         3183 g         4422 t
ORIGIN      
        1 aaaaaaataa tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa
       61 attattcatg tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt
      121 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
      181 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      241 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      301 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      361 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      421 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      481 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      541 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      601 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      661 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      721 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      781 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      841 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      901 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      961 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
     1021 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
     1081 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1141 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1201 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1261 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1321 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1381 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1441 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1501 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1561 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1621 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1681 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1741 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1801 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1861 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1921 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1981 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     2041 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     2101 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2161 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2221 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2281 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2341 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2401 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2461 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2521 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2581 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2641 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2701 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2761 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2821 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2881 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2941 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     3001 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     3061 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3121 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3181 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3241 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3301 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3361 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3421 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3481 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3541 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3601 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3661 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3721 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3781 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3841 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3901 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3961 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     4021 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     4081 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4141 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4201 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4261 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4321 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4381 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4441 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4501 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4561 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4621 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4681 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4741 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4801 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4861 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4921 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4981 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     5041 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     5101 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5161 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5221 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5281 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5341 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5401 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5461 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5521 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5581 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5641 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5701 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5761 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5821 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5881 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5941 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     6001 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     6061 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6121 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6181 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6241 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6301 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6361 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6421 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6481 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6541 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6601 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6661 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6721 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6781 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6841 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6901 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6961 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     7021 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     7081 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7141 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7201 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7261 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7321 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7381 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7441 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7501 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7561 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7621 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7681 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7741 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7801 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7861 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7921 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7981 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     8041 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     8101 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8161 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8221 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8281 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8341 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8401 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8461 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8521 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8581 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8641 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8701 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8761 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8821 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8881 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8941 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     9001 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     9061 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9121 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9181 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9241 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9301 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9361 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9421 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9481 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9541 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9601 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9661 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9721 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9781 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9841 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9901 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9961 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
    10021 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
    10081 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10141 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10201 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10261 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10321 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10381 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10441 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10501 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10561 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10621 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10681 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10741 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10801 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10861 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10921 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10981 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    11041 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    11101 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11161 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11221 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11281 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11341 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11401 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11461 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11521 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11581 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11641 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11701 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11761 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11821 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11881 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11941 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    12001 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    12061 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12121 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12181 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12241 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12301 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12361 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12421 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12481 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12541 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12601 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12661 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12721 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12781 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12841 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12901 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12961 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    13021 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    13081 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13141 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ataagaggat
    13201 gtgacaagaa ttttgtcaca tcctctttgt tttgttctca taaccttttt tcatatacag
    13261 ttcatgaacc gcttttatta acttgccaaa taacactgta ttgtgcgttg tatccagata
    13321 gaaagtctcc gtttcatctg aaattttctc atgctgatct gtaatgatca aatcagcatg
    13381 ttctgaatat gcggtaattt taacagcatc tttgcgaaat aactgctgca aatggctttg
    13441 aagatgataa tttcctgtaa actgtttggt tatctgcaaa tggatgagta actgctcttt
    13501 cgtttctgtt tgtagtaagt gttggatcaa ttgactaagg tattgactga tttggtttct
    13561 ttccccattt tttgaaaaaa ttgtatcgat tttttctctt aatttctttt cctgtataaa
    13621 agaactagaa tgattggacg tggcttctgg aaagcaaagt gtagaaaaac tactgctgga
    13681 ttcacccaac agttcgaaaa aaacaactgc caacacaaga aaataaaaat aaattggtat
    13741 tttattttct aaatggttga gaggaaactg ttgagttaag taagtcatca attgaccgac
    13801 ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct gtttttgtcg
    13861 aaaagagatt tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg ctctctctgt
    13921 ttctgtctct tcacaagggc gactcactga aaaagaagta aagtattctg aataatcatg
    13981 tgtatgctgc aataagtcca tcaattcagt gatttcagaa gaagtttttg gaaaatatgc
    14041 ctgttgtttg tttcgtaata tgaataaagt taacagcaag tagatatttt tttcttgttt
    14101 tgaaattttt tctatttgct catctgtact gttcattttt agtgcattta attcaagtga
    14161 ggaaaatggc caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa gatagagacg
    14221 gatagataac tcatttcctt caagagtaca gcaatttttt acagatagga tccgtaaatg
    14281 gaattttttt aaggaatgat tgagctgttt tgtcagtttt tcgagatagg agggcgatat
    14341 ataaagttgt tcaagtaaat atttttttgg tacagcagtt tgtggataat tgattagtaa
    14401 catcaaatag ttaaaacgat tggattcttt taggtagcat aattttaact tagaaaaaac
    14461 agctaacttt ttagtttgat cggtttcttt ggtttggatc tctcctttat gattagaata
    14521 aataaattca tcaatcaata aaaattgctt gagtgataca tttatttttc ggatcagttc
    14581 tttgattgtc cgttttggaa tatgtaagtc attggataat tcttgtaaag aacaattatt
    14641 tttttgaatg accatttgta gaacaagtaa ctcttttttt cttgcttcaa ttagcataaa
    14701 tacctccttt ttattctgtt tttgtatata gttagacaaa aactgtgagt ggaaagtcat
    14761 ttcagtctgt gttattctca cagagtgtcg ccttcttttt tatataacgt tatttaattt
    14821 ttttgttacc ttgtttattc tattgtttat ttcctagggt attttgtcaa gatattcttg
    14881 tttttaagg
//
LOCUS       DAZKCZ010000062        14838 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000062 DAZKCZ010000000
VERSION     DAZKCZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.062"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(293..859)
                     /locus_tag="ACG8KN_001733"
     CDS             complement(293..859)
                     /locus_tag="ACG8KN_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9188789.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(859..1815)
                     /locus_tag="ACG8KN_001734"
     CDS             complement(859..1815)
                     /locus_tag="ACG8KN_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9188790.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1815..2471)
                     /locus_tag="ACG8KN_001735"
     CDS             complement(1815..2471)
                     /locus_tag="ACG8KN_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9188791.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2841..4859
                     /locus_tag="ACG8KN_001736"
     CDS             2841..4859
                     /locus_tag="ACG8KN_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9188792.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4929..5135
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5286..7700
                     /gene="leuS"
                     /locus_tag="ACG8KN_001737"
     CDS             5286..7700
                     /gene="leuS"
                     /locus_tag="ACG8KN_001737"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9188793.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8048..9601
                     /locus_tag="ACG8KN_001738"
     CDS             8048..9601
                     /locus_tag="ACG8KN_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFP9188794.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9982..11766)
                     /locus_tag="ACG8KN_001739"
     CDS             complement(9982..11766)
                     /locus_tag="ACG8KN_001739"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188795.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11781..13502)
                     /locus_tag="ACG8KN_001740"
     CDS             complement(11781..13502)
                     /locus_tag="ACG8KN_001740"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9188796.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13602..14363
                     /locus_tag="ACG8KN_001741"
     CDS             13602..14363
                     /locus_tag="ACG8KN_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9188797.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4822 a         2732 c         2944 g         4340 t
ORIGIN      
        1 atgcttatat atcaatgttt ttgccattct tttatatatc tgttatatgt caaaaaaatg
       61 tgttcaattt caatttgtat ttgaataaag aggttgtgaa aaagctgtcc tgcatcaagt
      121 aataagaaca gccaatcaaa aacagcttct catgtttttg atttggttgg acttattacc
      181 gcagatataa gcttttgagc accgtgaata aagaggttat gaaatcaacg ctctaacctg
      241 agtattagag aaaaaaacgc aaaacatatc atttatgttt tgcgtttttt atttatcttt
      301 ttttctcaat acaatataaa ataggcggct ggttcttttg gttgataaac cgataattca
      361 gcacattata tgtttcctgt ggcagtttgg aacaaaaaga atcaaccata tccagttctt
      421 tttctccacc ttcatgacca tagtagacga caaggatcaa tcgtccgcga ggcacaagtc
      481 gtttcaatat ttcttccata gctgtgcgtg tagtctctgg catagtgatg attgctttat
      541 cactttttgg gagataccct agattgaaaa tccctgcttt gagattttgt tgttcagcta
      601 tcacttctcc taacgtttcg tggccttgta gaaacaatga gacacgttct tctaactgac
      661 gctgttctag cctttcacga gtagcatcga ttgcttgttg ctggatatca aaactataga
      721 catgaccagt ttttccgatg tgttctgcta gaaaaaccgt atcgtggcca ttgcccatcg
      781 ttgcatctac gacgtgatcc cctggttgca agatttcttg cagtaatgta tggctgaaat
      841 gcaaagcagt ttgcaacatc agacgctcac ctttttccgt acatcatatt ttccttggaa
      901 gctatctctg cgaagaagtt cttcatcaat tgcattcaag acttcccatt ttttcaagct
      961 ccacattggt ccaatcaagg aatcccacgg cgcatcccct gtcaaacgat ggatgatgat
     1021 ctcttttggt atcatttcca gctggtcgca aatgacattg acatagtcag gacggctcat
     1081 taattgaagg cgaccttcat gatagtcccg caacattctc gttttgcgca tcagatgcat
     1141 caaatggagc ttgattcctt ggatatctga atctaaaatg gtgcgtctga cattttcttt
     1201 catcatttct aaactttcgc ctggtaatcc gttgatcaaa tgcgtacaaa cacggatatt
     1261 atgtttgcgt aatttagcca cgccatctaa ataagtttga taatcatgtg ctcggttgat
     1321 cagttttgaa gtttcttcaa aagttgtttg caatccaagt tctacccata aatacatccg
     1381 ctcgttcagt tcagcaaggt aatcaactac ttcatctggc agacaatccg gtcgtgtacc
     1441 gactgataat cctacgacac ctggtaaatt gactacttgc tcaaatcgtt ctttgattat
     1501 ttcaactggc gcatgggtgt ttgtaaaatt ttggaaataa acgatgtatt gttggacatg
     1561 aggccatttt tgatgcatca tgtcgatttc tttctggaat tggattggaa gagggtcttc
     1621 aggtgcaacg atcatgtctc ccgaaccaga tacactgcaa aaagtacaac cgccatgggc
     1681 aacagtccca tctcgattcg gacagtcaaa tcctccatcg atcggcacct tgaaaatttt
     1741 cccgccaaac tcatttcgta aagcgtaatt ccaagaatga tatcttttat ttccttctga
     1801 ataaggaaaa gtcattaaaa gtccttcttt ctgtaagagt tatctatttg ttttcttttt
     1861 tctctataca ctggataagt caggcacagc caagaaagac tgagtaagta gccgccgatg
     1921 atatcagtag gaaaatgaac gcccagataa atgcggctta tcccgacact tactataata
     1981 aacatcatca gcaattgaag caatgtgcgt aaggttcttt tttgaacaaa agctggaagc
     2041 agaaatagta aagaaccgta aagaatcata ctgcctgctg catgtccact agggaaactg
     2101 taacttgttt caactactaa gtgcgccaat gaaggacgct ctcgcatgaa aaatagtttg
     2161 atcaccgggt tgatgattcc agcaattaca ataaatccag aaacgaacca aaatgtttct
     2221 gccctttttt tcttatacca catccataaa gccgcacaag caaataaaat gatgacggta
     2281 agaggattac cgaatttagt cataaagagt tggtagctgt tcagatcagg ataagaagct
     2341 ctgacaatgc ctgtaaacca ctgatcgaaa ccagaaagcc acgacagatg gaactttacg
     2401 atccagccca aaatgaaaaa caggacaaga aagaagctac cagccacctg aaatgatgtt
     2461 ttatatttca tacaattcct cctttgaagc aatgacttat tatagcattt ttggtccagc
     2521 gaaaaaaacg gtaaagagat aaaaccaatg tatgattcct ctttgaataa ccaatattct
     2581 tataaaaaaa ataactgtaa tcggaaagac attttttggt aatgcaaatg aaatgttagg
     2641 gaaataaaat cagtgttttt cgaaatcctt ttaaacaaga acgtagacgt aggaagaact
     2701 tgaaaatgaa cagaagttgt tcattacggt aaattacaat atgattacaa aaaacaacaa
     2761 agctcttaaa taacttttaa taaatattaa gagaagttat aattattaat aatagataaa
     2821 ttgaagttgg aggatagaag atggaaaaca ttgcaagaaa agaacggaga cgtttagaac
     2881 agaccaaacg ttttcgaaaa gcaaaacgag gggcagcaat cgtgggaact gcgatggtag
     2941 gctgttctgt cgctgcccca ttagtacagc cggttcctgt tcaagcggat gaaacacctg
     3001 ttcagttcgg cgcaagaatc aatacagcgg catttatcac agaaattgct acttatgctc
     3061 aaccaatcgc tcaagccaac gatctatatg catcagtgat gatcgcacaa gcagttgtag
     3121 aaagcggttg gggcagtagt gcgttatctc aagcacctta ttataatctt tttgggataa
     3181 aaggcagcta tcaaggacaa accgtctaca tggatacgct tgaattttta aataatcaat
     3241 gggtaaataa aaaggagcct ttccgacaat atccttcttt tgctgaatct ttcaatgaca
     3301 atgcctatgt acttagaaat acttcatttg gcaacggtta ttactatgcc ggtacttgga
     3361 agagcaacac gaaatcctat acagatgcga cagcttgttt gacagggcgt tatgcgactg
     3421 atcctggtta cgcaggcaaa ttaaataata tcatcgcaac ttacggattg acccaatatg
     3481 atacaccagc tagcggaaac gcaggcggtg gagcgactac aggtggcaat aatactggaa
     3541 gtgcaggtag tactggcggt tcaagcaatt ctggtaatac aaactcaggt tcagcaactt
     3601 cgaccactta tactgtccaa tctggtgatt ctgtttgggg aatctcaaat aaattcggta
     3661 tcacaatgga tcaattgatc caatggaaca acatccaaaa caatttcatt tatccaggac
     3721 aaaaactgac aatcaaaggt agccaggaaa atggctcttc gacgaataat tccggtaaca
     3781 ataccaactc ttcaggaaat gctggtacat caaacggagg acaaacaact ggtgcgaaat
     3841 acactgttca atctggtgat tctgtttgga agatctcaaa tgatcacgga attacgatgg
     3901 ctcaattgat tgaatggaac aatatcaaga acaatttcgt ttatccagga caacagctaa
     3961 tcgtatcaaa tggcggttct tctaatgctg gagcaaatac tggctcaaat actgggaata
     4021 caagtggctc tagttcgtcc agctcaagtt cttctaatac agcttcagga acgaaatata
     4081 cagttaaagc cggagactct gtttggagcg tctcaaataa atacggaatt acgatggctc
     4141 agctaatcca atggaacaat atccaaaata actttatcta cccaggacaa caactgactg
     4201 tttcaaacag tggatcaaca aacagcacgt caggtaatac aggttcgtca accaatacaa
     4261 ataacagcag ttcagttaat actggcagca gttcgtctca aggcacttat accgtgaaag
     4321 caggtgaatc tgtttggagc gtggcaaata aaaacggcat cacgatggat cagttaatcg
     4381 aatggaataa tatcaaaaac aacttcattt atccaggaca gcaattgatc gttaaaggtg
     4441 gaacttcggc aaatacaaat acaggttcaa ctacttctgc agcgaaacca aatacaccga
     4501 atacgtctgc tacgacaagt acatcatcca ctggtaatac gatgtatacc gtgaaagcgg
     4561 gcgagtcagt ttggagcgta gcgaacaaac atcatatcac gatggatcag ttaatcgaat
     4621 ggaataatat caaaaataat ttcatctatc caggacagca agtgattgtg aagaaaggtt
     4681 catctcaatc aacaaaccaa caatcagtta ctggttcgaa aacctatact gtgaaatctg
     4741 gagaatctgt ttggagtgtt gctgacagtc atggaattac aatggctcaa ctaatcgaat
     4801 ggaacaatat taagaacaac tttatttatc caggtcaaac gttgattgta gctaaataaa
     4861 atagctgttc gaagaaataa cgattgaatt ccacaaaaat atttgttact ataaggctac
     4921 ttaaatatga caacacttga aaaggaagta gtaaaaagac tgaagtttca gagagcgcat
     4981 ggctggtgag aatgtgcacg gatgcttttg aactcgcctt tgagttgccc ttgctgaata
     5041 cccgagtaag caagcggcgg tgacggccgt tacaacgttc gaggcctttt cactaaggta
     5101 aaattaggtg gtaccgcgta gattacgtcc tataaaaaga gcaatctttt tataggattt
     5161 ttttatttct tttgtgaagt ttgagaaaac aaagcaaaag gaaataaaaa taaacagatt
     5221 ctgccggttt tttcgttata tttataatag aaacaagtgt tttcaataga acaggaggac
     5281 agcaaatgag ttacaatcat aaagaaattg agaaaaaatg gcaaaaatac tgggcaaaaa
     5341 cgaatacttt caacacccat gatgatcctg aaaagccaaa attttacgca ctggatatgt
     5401 ttccctatcc atccggtcaa gggctacatg tagggcatcc tgaaggttat acggcaacgg
     5461 atatcctttc acgtgtgaaa agaagtcaag gatacaacgt gctgcatcca atggggtggg
     5521 atgcattcgg tctgcctgct gaacaatatg cgctagatac aggaaacgat ccagctgagt
     5581 ttacccaaaa aaatatcgaa acattccgac gccaaatcaa ttctttagga tttagttatg
     5641 actggaatcg cgaagtgaat acaactgatc cggagtatta taaatggaca caatggatct
     5701 ttacgaagct ttatgaaaaa ggtctagctt atgaagcaga agtagcggta aactgggtac
     5761 ctgaattagg aacagttatt tcaaatgaag aagtgatcga tgggaaaagt gaacgtggtg
     5821 gctatgatgt agtcagaaaa ccaatgcgcc aatggatgct gaaaatcact gcttatgctg
     5881 atcgcttatt ggatgatcta gaattagttg attggccaga aagcattaaa gaaatgcaaa
     5941 gaaattggat cggtcgttca gtaggagcaa atattgaatt taaagtggct ggtacggata
     6001 aatcctatac cgtatttacg acacgaccag atactctatt cggtgcaact tactcggttc
     6061 ttgcacctga actagatctt gttagagaaa ttacgacccc tgaacaaaaa gctgctgtag
     6121 aagcttatat cgaagagaca gctaaaaaat cagatctaaa gagaacggat cttgcaaaag
     6181 aaaaaacagg agtatttact ggtgcttatg caatcaatcc agttaatggc aaggaaatcc
     6241 caatctggat cgccgattat gtattatctt catacggcac aggagccatc atggctgttc
     6301 cagcacatga tgaacgtgac tttgaattcg ctcaaacttt cggactagaa attcttccag
     6361 ttatcgaagg tggagatgtt gaaaaagctg cttacacaga agatggtacg cacatcaact
     6421 ctgaatttct agacggcatg aacaaacaag aagcaatcga taaaatgaat gaatggctag
     6481 aagaaaatgg cgtagggaaa aaagaagtca gctatcgctt acgtgactgg ctattctccc
     6541 gtcaacgtta ctggggtgaa ccaatcccaa tcatccattg ggaggacgga acagtcacag
     6601 ctgtcccaga agaagagcta ccattacggt tgcctaagac aaaaaacatc aagccaagcg
     6661 gtactggaga atctccatta gcaaatatcg aggagtgggt caatgtcgtt gatcctgtaa
     6721 caggtaaaaa aggccgtcgc gaaacgaata caatgccaca atgggcagga agctcatggt
     6781 attacctgcg ttatatcgac cctcataaca aaaaagaact agcagattat gaaaagttga
     6841 agcgttggct accagttgac atctacattg gtggagcaga acatgcggta cttcatttac
     6901 tatatgcacg attctggcat aaattcttat acgatatcgg tgttgttcct acaaaagaac
     6961 cattccaaaa actatacaat caaggaatga tcttaggcga aaacaacgaa aaaatgtcaa
     7021 aatctcgtgg taatgtagtc aatcctgatg atgtggtcga aacttacggt gcagatacac
     7081 tgcgcatgta cgaaatgttc atggggccat tagatgcttc gattgcttgg agcgaaaatg
     7141 gcttagaagg aagccgtaaa ttcttggatc gtgtatggcg tttgatcgtt gatgagaaca
     7201 ataaaatgcg tgaccgtatc actacattga atgatggcaa gcttgataaa gtgtatcatc
     7261 aaacagtgaa aaaagtgaca gaagactatg aaaatcttca tttcaataca gcaatctccc
     7321 aattgatggt ctttatcaat gaagcctata aagtggacgc tctgccttat gaatatatcg
     7381 aaggatttgt tcaactactc gcaccgattg ctcctcacat gggtgaagaa ctatgggcaa
     7441 tccttggaaa tgatggtggt atttcttacg caccatggcc aacatacgat gaagcagcac
     7501 ttgttgaaga cgaagtagaa gttgttttcc aagtaaacgg taaagttcgt gcgaaaagca
     7561 atgttccccg agacttagga aaagatgagc tagaaaaagc agcattagcc aatgaaatag
     7621 tgcaagaata tattgaaggc aaaacagtca gaaaagttat tgcggtacca aacaagttag
     7681 tcaatatcgt tgctaattaa ttggctcttt gtcaataagg actgatgagt tgtgcaaaat
     7741 caaatctggg ccagaatgaa cttcattctg tctcagattt tttgttattt caaattgatt
     7801 aattgattga tcaaaaaggt tttgattttc atgttctcaa acacgttcaa tgtctttttc
     7861 ttctgaatgg tcttgtaaga gatctctgta atcatcaaat ttttgtctat taatcttagt
     7921 attttttgat gtataaacta cctgaaattg agagtacata ccaaattcat aaatttttta
     7981 ttagtgggac tagtgattgt atggattttg acgcataatg aatgaagtat gagaaaagga
     8041 gagacaaatg gatcaagaac tatttattat cgaagtatta tcagctaaac aaaaaattat
     8101 cgagaaagcg aaaaaaagag cgaaaagaat aatgttcaag cttttaagct tagtattttt
     8161 ctctggtttg ctttttttcg gagctgtatc gttcctatat cgccatatga gtgcagtagt
     8221 ccgttcagct gaagggatgc aaaaattaaa taatttgtat cacttttcgt ggggagttgg
     8281 cggaataaca attgttgtgc ttattgtttt tcttctactt atttggctcc gttaccatca
     8341 attgtcgcgt catttcgatc aatggcttag tcaaagagca caacagtttt tacataataa
     8401 accaacttct caagatgatg actatcttta ttttgagaag acttcgacga gtcaagcttt
     8461 tcggatacaa aaaagtgatt gccagttatt gctgagatca cttgatgatt tacctatcta
     8521 cttaggcgaa agcaaatcaa aattcggaat gaacacattc catatttttt tactagaaaa
     8581 cgaacccaga tgggaaaaaa gccagtcttc gcctggtgga tacctgacaa ctaagatgag
     8641 tacattagtc actggttttt tcctcattct tttagctgga acaatctatt atgggagata
     8701 cactcagcca aaaatgtctt ctcatataaa tgctgcttct cacatgacaa tgaactcaac
     8761 taacgatacg catatacaaa agacaaatga agggttgtta gctgatccta agcaaaaggt
     8821 aaatttgaca acacaaaaga taaatgatct caaacttgat acagagacgc actctctcta
     8881 tatgacaaca gatagtggtg ctaattgggt atttgtgccg attgattcgc aatggttgcg
     8941 ttttggcgaa tacaccctga caacggggct gattcctgaa ggttattgga tggacaacac
     9001 atatgatatt tccgccgatt tctcatggtt tatctattct tctggggatg atgtacgtct
     9061 gctgacttca agagataatg gtaaaacatg gcaaacgtct attatcaccc aatcagttca
     9121 gccgatacgt tatcgaaaag cggtatttta tggcaacgat ggtctattta tctgtacatc
     9181 agccagtggg atgtctgcag aaactttgta catttacact actatggatg gcggaatcac
     9241 ttggataaaa agtggaagta caatgatcaa tcagccagta caaaatgtca gcttcgttac
     9301 aacttcttta ggttttgttt cgacacgtga aagtttgttt tatacaaaca acggaggacg
     9361 ctcattcaaa gaatcagttc tgtcgattcc ggaaaactat cagacagggg ggatagatct
     9421 ttttcaatca ccaaatgaag ttactcaaat aagcgcgaat acgttagaga caaaatttta
     9481 tttgttgaaa aaaggaccaa tcgatgcagg gaaaatgttt gcctgcctct tccgttcaac
     9541 tgataacggg gaaacatggc aattcgttca gcaactatcg caagtagaac aaggagatta
     9601 aagaagagag attgtgagcc tgacgttcag caccgagtca caagaaacaa attcaaaaaa
     9661 cagcttctca tatttgttga atagtgtaac tcgtgattcg gagttgacaa gagcaacaag
     9721 aagcgatttt gaagtatagc ttctcgtatt ttacaagatc gtgtcttatt gccgataggt
     9781 tgtctcttga acaccgttta ttcgtaaaaa gggattgtga caagtctttt gtcactactt
     9841 accgaacgaa actgaaggag ctatccaaaa agtatcatta gtattattct ttgtgtttct
     9901 agtggcacca ctagtacatt taaaaaagct atcgatcatg gactaatcat acatgatcga
     9961 tagcttttta gataatgttt gttacgctat ctgaaattga ctattataaa gactggcata
    10021 aagcgttggt gattggagta gttcagaatg tgttcctttt tcgacgatat cgccattctt
    10081 cattacaagg atcatgtctg cattttcaat cgttgacaaa cggtgtgcaa tcacaaaact
    10141 tgtcctatct tttgtcacag cttccattgc ttgctgaatc aatgcttctg ttcgtgtatc
    10201 tacgctagaa gttgcttcat ctaaaataac gacaggagga ttcgctagaa tgactcgagc
    10261 aattgtcaat aattgttgct gtccttgaga taacgtgccg ttttcgctag aaatgatcgt
    10321 atcatatccg tctggcagtg ttcgaataaa gtgatcacat tgtgcgattt cagccgcttc
    10381 gataatttgt tctcttgtgg catccatttt cccgtaggcg atattatcag ctaccgttcc
    10441 ttcaaacagc caggtatttt gtaaaaccat cccaaaagaa cgacgtagtt ctccacgtga
    10501 aagcttggta atatcaagac cgtcgaattc gatctttcct ttattcagtt cataaaaacg
    10561 catcagcaga ttgactaaag tcgtttttcc tgcaccagtt ggaccaacga tcgctaccgt
    10621 ttgttttggc tttactgaaa aatcgacttg attcatcaat attttatctg atgtgtaacc
    10681 aaactgtaca tttttgaaag ctatttttcc tttcggttgt tctaaaagtc ttgcatctgg
    10741 aaggtctgga atttcatctg tttcatctaa tatctcaaat atcctatcga ttgctgccat
    10801 tgcggattgg atcgagttga tcacataaga agcggtagaa attggttcag aaatctgatt
    10861 gatgtactga agataagctt gtaagaagcc gatcgtcaca ccacctgata gaacaagcat
    10921 agcaccgaat accgcactta tgatgaaggc gagttgatta atgaaacgaa tggccggata
    10981 gatagcacag ttcataaatt gagcacgttt aaaagcgttg tattgctttt tatttacttc
    11041 gtgtatagca gcttctgcat gagcttgctg attgaatgtt ttgaccacta gattaccagc
    11101 taagatttct tccattcgat tatttaaaac acccagttca tactgattct gttcagaaat
    11161 ctgtttgttt ttgttcgcaa tcttagttgt aacaattgaa ctgccaacga tcagcaataa
    11221 gacgagcaaa gtaagcttgc tttcgatata aaacagcatc agaccagcag cagctattgt
    11281 tacgacagaa gtaaaaaatt gattgatacc tgtcaataac acttgagaaa gttgattgag
    11341 accggtagtt gtccgactaa tgatgtcacc tacttgatga ttgtcaaaaa acgacatcgg
    11401 taatgttttg aatttttttg tgatttcttt tcgcaccctc aatgtgatct tttcgcttaa
    11461 cgctgccatt gtccgttcct gaataaatga agtgatgctg cttaagactg aacagccgat
    11521 cagcaataag acagggaaca acaatgcttg tttgatatca ctgaaagaaa gctgtccaat
    11581 acctgttttt cttatttctg ataagagatc atcgatcccg attcccatga tcaagggcat
    11641 ggctacgact aataggttcc cgaataaact gcatagaaac aatccgtaaa aatatttatg
    11701 ttcaggtttg atcattttcc aaaaacgtat caaagcacgc gtgttcaacg tttttttctt
    11761 ttccatcgta tcccttcttt ctatattcct tgtgattttg caaactcctg ataagtttgg
    11821 caattagaca gtagttcttc atgagtacct tgtccaacga tttttccttc atctaaaaca
    11881 atgatgttat ctgcttgttg gattgtactg aggcgttgag caacaatcac gagagttttc
    11941 tccgacatgt gtttatgaag ggctgctctt aatgctgcat ctgttttata gtctaaagcg
    12001 gagaaactat catcgaatac atagatctct gctggtttga tcaacgctcg agcaatacat
    12061 atccgctgtt tctggccccc tgaataatta gaaccgcctt gagcaacaaa gctgtccaat
    12121 ccgtcaggaa gggtgtccac tagttcttct aattgagcga tcttgatcgc tttagacaac
    12181 tcttctgttg aggcatattc atttcccatc tgcaagttgc ttaaaattgt cccgctgaat
    12241 aagaaagcct tctgagggac ataactgatg tgggagcgga tctgttcttg agtcatttga
    12301 gtaagaggaa taccaccaag ggttactttt cctatcgtag ggtcactaag tctcaagaga
    12361 agtttcgcca aactactttt tccagatccg gtcccgccaa cgatcgctgt tgtttttcct
    12421 ttaggtattg tgaaatcaat atcttctaga actggatcag agatttcagt atatttaaat
    12481 gtgacatgtt caaaagcagc tgcatttgtc tgatcaaagg ttggttcagc tactgttttg
    12541 atcgtctctt cctcaatttc gatctctgct tgcagtattt cttctatccg tcgtaatgag
    12601 gcaaacgatt tagggataat gaccaatacc atagctgcga tcatcaagta agcaagtgtc
    12661 agcatagagt attcgactac agcagtcacg acaccgattt gtaatgctcc ttctgctaca
    12721 agatttccgc caaaccacaa aatagaagca taaacgattc ccatgagcaa aaaagcaatt
    12781 ggagtgaccc aagcaaatat tcggttcact ttgatcattt gattagcata gtcagcaaag
    12841 ctcttattcg ttcgcttttc ttcatatgat tgattatcaa aggcacggat catattaatc
    12901 ccagtaaaga attctctcaa tgtgatggtg atccgatcca tttttggctg gatactcaaa
    12961 gataaaggtg cccctcgttt catcagtcga taaacaatcc atacataaaa aacaacagag
    13021 atcaatgtaa cccatgttag taatagagaa taacgaaaca tcatgagaat ggtgaaaacg
    13081 caaataaccg gtgctggtaa gatcatttgg caaaataaaa caatcatttg ctggacatta
    13141 tcgacatcat tcgtcattct tgtcaaaaga gatccagaac taaaacgact gatatccttc
    13201 attgacagct gttggaattt acggaaatat ttctcccgcg tttggaatcc aaacctagcg
    13261 gaagccaatg ctgaaaagta actagaggca actgcagcga ttgttcctag aaacgctaca
    13321 agcagcattt caactccaat tttttttata actgaggctt cgccacttgc tatacctgtg
    13381 tcgatcagct ttgctactaa ttttggtaca cctaatgtac cggcgatttc taaacagatc
    13441 atcacaaagg taaggagagc gagtcctttg tttttctgta agaatgtttt taagatagac
    13501 atattattcc tccttttttc ctgatacttt tcgattctat accttcaagt aacttgaagg
    13561 tcaatagaaa aaagaataaa ctgaaaaaga ggtgagaaaa aatgaaaaaa atgtatacga
    13621 ttggccaagt atcggaacta tttgatttgc caaaatcaac gatccgctat tgggatgaac
    13681 aaggattaat ccgttcaagt agacaagaag agaatgatta tcgattgttt gatatcgatg
    13741 atatttttat gctttatgat atcgtttttt atagaaaatt agatataccg atcaaacaga
    13801 tgaagaattt atatggaaaa acattgacgg agttatatga gacacttgat gaaacagaaa
    13861 gaagaatcca taaagaactg gtggtgatga aacaaaaaca aaaagagata agggagcaaa
    13921 aaaaacagtt gaaattgatg atcgatacaa acgaggaaga atttcctgta gaagagatcc
    13981 cttttgactg tatgatctca acggagtttg aagatattgt ggaaatcaaa aaatttctac
    14041 ctaactattc aagctttggg atgatgagta tgccagcttc cagttcacag acgatgtatg
    14101 gattttttat cgaccctagt gaagtacatt tatttactca agacgtgatt tgggagaaaa
    14161 aagatacggc tgtttatcgt cgattcttat tgaaatccga gatgaaccat gcggaacgta
    14221 ataatatttt agaaatcaga gaaagaatgt atgaaaaagg atggaaaaca ggagaagtga
    14281 tcggccagta tctattgacg aatacggatg aaaacaatat cagaactgaa tattatcatg
    14341 catggataga aatgaaaaaa taggaaatat gtgtttaaga taactcgaaa atgatggttg
    14401 ttttagttat aaagaaaaat tttctggttc gattacgggt ttaatgtggg ggattattag
    14461 tcgtggatag atagaatttt agttttttag aggaaatatg tctttaaaaa tggaatggtt
    14521 gcaattctta gcgatatata cgatgtaatt gtcctcttaa aaaagtatgg aaaaataatt
    14581 gtattgggtg ttatgtataa ttcatggtga actgagacta atgagaagaa aaattcatat
    14641 tgttatgagt gagattgtat cagatgagaa tgataaatta aataggtgca taaaagcatt
    14701 acacttaaaa tacattgttg cttacattat tacaatatgt taataaggca aaaataaata
    14761 gatggattga ataaacatat tttttaatca aatagtccct actgtgactt tcctcagcta
    14821 tcggtgatac taccgata
//
LOCUS       DAZKCZ010000063        14600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000063 DAZKCZ010000000
VERSION     DAZKCZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.063"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..293)
                     /locus_tag="ACG8KN_001742"
     CDS             complement(18..293)
                     /locus_tag="ACG8KN_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188798.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            587..1402
                     /gene="thiD"
                     /locus_tag="ACG8KN_001743"
     CDS             587..1402
                     /gene="thiD"
                     /locus_tag="ACG8KN_001743"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9188799.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1607..3058)
                     /gene="cls"
                     /locus_tag="ACG8KN_001744"
     CDS             complement(1607..3058)
                     /gene="cls"
                     /locus_tag="ACG8KN_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9188800.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3400..6066
                     /locus_tag="ACG8KN_001745"
     CDS             3400..6066
                     /locus_tag="ACG8KN_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9188801.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6430..7182
                     /locus_tag="ACG8KN_001746"
     CDS             6430..7182
                     /locus_tag="ACG8KN_001746"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9188802.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7204..8358)
                     /locus_tag="ACG8KN_001747"
     CDS             complement(7204..8358)
                     /locus_tag="ACG8KN_001747"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFP9188803.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8543..10984
                     /locus_tag="ACG8KN_001748"
     CDS             8543..10984
                     /locus_tag="ACG8KN_001748"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFP9188804.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11314..12018)
                     /locus_tag="ACG8KN_001749"
     CDS             complement(11314..12018)
                     /locus_tag="ACG8KN_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188805.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(12205..12450)
                     /locus_tag="ACG8KN_001750"
     CDS             complement(12205..12450)
                     /locus_tag="ACG8KN_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188806.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12467..13318)
                     /locus_tag="ACG8KN_001751"
     CDS             complement(12467..13318)
                     /locus_tag="ACG8KN_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9188807.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13484..14488
                     /locus_tag="ACG8KN_001752"
     CDS             13484..14488
                     /locus_tag="ACG8KN_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFP9188808.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4695 a         2558 c         3034 g         4313 t
ORIGIN      
        1 taaagaacag ttagaagtta aaattgctga ttcacataga gtttatgact tcttggaaat
       61 tctttttcaa tcagtttttt catctgttta tgaaattctt ttttttcagt aaacataaag
      121 gtttgggtat cgtaaccttc ttcatgtagg aaatagatta ttcctttttc tgtgttttgc
      181 gtataagctt ttatactcct gtcttttttg aaagaacaga tcgtatagcc atctatttgg
      241 tctggtagtc gtttgatttt cttcaaaatg acttttttat cctcaagttt catttttttc
      301 acctgacttt atttttttca tatggaaatc atagcgcctt tggttatatg aaaacaagaa
      361 ggatactgaa atatatataa atcaaatagt ggttatttat tattagaata gcgaatacaa
      421 acaaatgaca actattttct gatttattac aaatgttttt tcaaataaat ttgattacca
      481 agaaaaggta gtggtatatt tactgaggga tactttgaaa aacatatgat agaaacagta
      541 acagcattat gaaaaatctt tttataaaaa tggaggaatc gatttgatgg aagatcagta
      601 tgtacaagca atgacgatcg caggatcaga tagcgatgga agcgcaggga tgcaagcaga
      661 tatgcataca ttctttacaa gaaatgttta cggtgtttct gtcatgaccg catgtgttgc
      721 aggtaattct tacggaatcg gagcaagtgt gacattgcca acagatttta tcgacaaaga
      781 atttgaactg attgcgaagg actttcaaat acgtgcagct aaaacaggta tgctagcaga
      841 ttcaaagttg attgagacag ttgtaaaaaa ttaccaaaaa tatgattttg gaccattagt
      901 cgtggatccc gtaatcgtaa cgaaacatgg aaatctttta ctagaagaat ctgctttgca
      961 aagcttgaaa gaaaagcttg tccctttggc cgaagtactg acacctaatt tttatgaagc
     1021 agaaaagctg actggacgga cattagcagc tgaagaagat tttgtgcaag cagcaaaaga
     1081 actgcaagat atgggtgcaa aaaacgttat gatcaaagga catcatgcta aagatgcgca
     1141 gcaagaaaca gtcaatgact acgtattgct cgaatcagga aaatcatttt ggttaaatgc
     1201 accattttat cctaccgacc ggatcaacgg aactggcgat tcgctatctg catgtattgc
     1261 agctgagtta gcgaaaggaa aaacagttga acaagcgatc cgattggcaa aagactatgt
     1321 aaaccgtgca attcgtgaag aaataaatgt agggcacaaa tatggaccca tcaatcattg
     1381 ggcagctggt gaattagagt aaaagaaaga ggtcgtgagc ctgccgtcca gctccgagtc
     1441 acaaggaaca agttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg
     1501 aggagttggc aggagaacac cgtttgttcg gaagaaggga ctgtgacaag atttttgtca
     1561 cagtcccttt aatcttagta tatttttctc tacttgaata aaaaaattat aggattggag
     1621 aaaataatcg agaaatttgt tgcttgaata atagccagtt agacatttcc ttgactgttt
     1681 cagttgtcat ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca agttcttcta
     1741 aaaactcagg atcgtaaatg acagcacttg tttcaaaatt caatcggtag ctacgaatat
     1801 cttgatttgc tgaacctacc atgcagattt catcatccat tatcaatgtt tttgcatgga
     1861 ggaagccgtt ctcatagaca aggatttgta cattttcctt gatcagctgc cgagcataat
     1921 attgtgttgc tcgataaata aaaggatgat ccggtttgtt gggaatcatg attttcacat
     1981 ctacaccaga ggcagttgcg atttttaaag cagcaatgac actttcatca ggaacaaggt
     2041 aaggtgtctg tatccaaaca cgttttttag cagaagtaat caatttgata aatgaaagct
     2101 tgatttgttc cctttcgtta ttcggtccgc ttgaaacaag ctggatggat gtatcagcaa
     2161 gcttagattc atctcgatct gcttttttaa agaaataatt caaatgatag ccgacctttt
     2221 tttcttcagg taccgagacg ttccagtctg ttaagaagcg aagctggagc aaagaagaag
     2281 cggcaccgaa aatccgtata tgcgtatcgc gccaatagcc gaattttttt gttgtattta
     2341 catattgatt ggcaatattg aaaccaccgg tataactaat ctttccatcg atcacaacga
     2401 tttttcggtg atcatggtaa ttcaatcgga aacgaagtaa tgccttttgt gaggtaacaa
     2461 atgtatggac atgtccacca tttttgatta gacgattgaa atcttttgct tttgtaccat
     2521 gagagccaaa tgcatcatat aatattcgaa cttccacgcc ttctgcggct ttttcttcta
     2581 aaacatgtaa gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat tcgatatgga
     2641 tcgaatgttt ggcttttttt agatcttgaa gcagtgaatc caatttctct tgtccgtctg
     2701 tgagaagagt gacagaattc attcttgtca gcggcatacg atttaatgaa gagaagaagt
     2761 cgacaaattg ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca atggtatctc
     2821 ctctaaaaga ctgaaagttt tctaattctt tcaagtcact ttgtcggaga tagaattttt
     2881 tcttatccgt taatccacgt ccgaaaaata aatataatac aaagcccacc ccaggaaggg
     2941 caaacaatac taagagccat gcccaaatgg ctgctacatc tcggggtttg atcaaaatag
     3001 ccaccaaagc aataagtgca tttaatagat aaagggcggt tataatacta gataccacaa
     3061 aaatgttcct ccttacatac aataacatta gtatagcatg atagaagaaa gaagcctacc
     3121 aaggtgaata tacttttcac tcctttcatg aaaatgtagg taaatcgggc taaagtagtt
     3181 gctttttgta ggttaataag gtaggatatt agtacagttg aatatctcct taggggagta
     3241 accggcaacg tttgttgcga taatatcaac agtaagttaa ccaaatggtt ttctggtatt
     3301 gtatgaaatg gtgagaccta agacaaatgc gtatttgtct taggtctctt tttttttcag
     3361 acaggactag ggccaagatc aaaaaatggg ggatgaaatt tggaaaacaa ggaacaagca
     3421 caacaagcgg tctataaaga aacgatcgac tcgttgatgc agcaaacgca gagctcaacc
     3481 gatggattga gcaccacgca agcgaaggaa aaattagaaa gagatggctt gaaccagcta
     3541 aaagaaaagc cgaagaagcc aacttggaaa ttgttcttag aaactttcaa agatgcaatg
     3601 gttatcgtat tgctgattgt cgcactgatt cagatggtca tgggtgacta catcgaattc
     3661 ttagttattt ttgcagtatt gatgttgaat tctataatca gtgttgtcca aacaaaaaaa
     3721 gcagaaggtt ctttagaagc gttgaaaaac ctttcagcgc ccgatgctcg agtgattcgt
     3781 gatggtaagg aaaaaacgat tccggcaaac gaacttgtgg taggagatat cgtgttgtta
     3841 gacgcaggcg attatgtacc tgctgatgga cgtttactag atgcgggatc attaaaagta
     3901 gatgaaggta tgctgacagg agagtcgact tctgcagaaa aagaagtaac ggatatcaca
     3961 agtacggtac caatcggaga tcgtacaaat atggtattca gtggaacgat cgtaacttac
     4021 ggacgcggta aattcttagt gacttcaact gggaataata cggagatcgg acaagttgct
     4081 ggccttttgg aatcaacaac tgagcaagtc actccgttac aacgaggtct ggataaattc
     4141 agtaaaaaac taagtctggc tattttggca ttgtctttat tgattttagg tatccaactg
     4201 tttcgtattt atcttggaga aggtacagga gatatgactg cagatatcgt cagcgcagtg
     4261 atgtttgccg tagccgtagc cgtagctgct attccagaag cattacagtc tatcgtaaca
     4321 atcgtgttat ctttgggaac gaataaaatg gcaaaacgtc atgccattat tcggaagctt
     4381 ccagcagttg aaacacttgg ttcgacaagt atcatctgta cagataagac tggtacattg
     4441 acacaaaaca aaatgacagt tgttgactac tttttaggta atggcgatac tggggatttc
     4501 acaaatgacc cttcaaaatg gacagctgac gaacgtcgct tgatcgaaat cagtgtttta
     4561 gcgaatgatg catcgatcag tgaagacggt actaaattgg gcgaccctac ggaagtagcg
     4621 tttatcgact ttagtgaaaa attaaaccag ccttatcagg aaatccgaaa caactaccca
     4681 agacaagcag aacttccttt cgattctgat cgaaaattaa tgtctactgg acacacgatc
     4741 gataacgaaa attacttgtt tgtcaaaggc ggaccagata tcgtcttcca acgcagtaaa
     4801 aaagtattgc taaatggtga agtcgttccg atgacggatg aattgatgaa aaaattccaa
     4861 gagcaaaatg aagctttttc aacacgcgca ttacgtgtac tagcttttgc ttaccgtcca
     4921 cttgataatt ttgatttatc atttgaagat gaagacgatt tgatccttgt tggtttgctt
     4981 gctatgatcg atcctccacg tgctgaagtg actcaggcgg taacagaagc aagaagtgca
     5041 ggaatcaaaa caatcatgat tactggtgac cacaaaacaa cagctgttgc tatcgcaaaa
     5101 caaatcggga tttttgaaga aggaaacctg gctttgaccg ggacggaact agatgaattg
     5161 acagaagatc aactaaacga agttttagaa aaagtcactg tttatgcacg tgtttcacct
     5221 gagaataaaa ttcgtatcgt tcgtgcttgg caaaataaag gtcatatctc agctatgaca
     5281 ggtgatggtg taaatgacgc accagcattg aaacaagcag acatcggaat tgctatggga
     5341 acgggtacag atgtggcaaa agatgcagca gcgatggtat tgacggatga taactttgct
     5401 tctattgtca gcgcagtaga agtaggacgt aatgtgtttg ataatatcaa gaaagctatt
     5461 tcttatctat ttgcaggaaa tttaggtgcg atcattgcga tcatctttgc cttagttgct
     5521 gactggtcca atccatttac agcattacaa ctgttgttca tcaatttagt caatgattca
     5581 ctgccagcaa ttgcattagg tacagaaaaa gctgaacctg ctacgatgaa ccgcccgcca
     5641 agagatccaa attcaggaat ctttacggac aaaacattga tttctgtaac gtatcgtggt
     5701 gtgctgattg ggattgctgt tatctttgct caatggcttg gcatgcagca ttcacctgaa
     5761 ttcggtgtag ccatgtcatt ttcaacattg atttggtgtc ggacattgca aacattccca
     5821 gcacgttcaa acacacaaac agctatccaa gcagggttct tctctaacag aagtgtctta
     5881 ctagctgtag ttgtatgtag cgcgctttac tgcttaacaa tgatcccagg actgaaagaa
     5941 gtcttctcta tcccatcagc atttggattg acagaagtct ttacatgtat gggaatcgca
     6001 cttgtgacaa ttgttttgat ggaaatcact aaattgatca ttgtacgagt acaaggaaat
     6061 aaataaaacg aatcaacgag gttgtgagcc tgccgtttaa ttctgagtca caaggtgcaa
     6121 tttcaacaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga agagttggca
     6181 ggagaacacc gtttaaggaa gaagttgtaa aatcagcatt ttgagctaat actgagggtc
     6241 atctgattgg acactgttta ttcggaaaga gggactttga cacgactttg gtcacagtcc
     6301 ctttttttat ttatgataga cggaaagcca atcattttat tattcatcaa aagagataga
     6361 atagataaaa gaggagaata atttattccg aaaagtgaaa acatataaca aaaaattaga
     6421 ggaaatcaaa tgaatgaaaa agaatgggat gaatttgctg aagaatatta tgaaatccag
     6481 caagaatcgc aagtaactat tgctgaagat gtaaaaaatt atctgaaaac ggagaaagtc
     6541 ctgcctgctg aaacagcagc agacgttgct ggaggatctg gcagatttct ttccttagca
     6601 aaagaaatta atcgctatga tttgatcgat ttttcttcag gaatgctaga aaatgcaaaa
     6661 aaagaagcag aaaaaaatga gttgccaaat gtttccttta tcaaacagtc gttagaagaa
     6721 tttatccaat caaatgagac atatgaaatg gtttttacag cagcaaatcc agcattggta
     6781 catcttattc aattagaaaa attgatgaaa aagtcaagca aatggtgcgt gataatcagg
     6841 gtagtggaat caatcgatga tgttttttat cctatcgaaa agcaactagg aatgatagaa
     6901 gaagatccta atacatcgcc agttgtaatg gatcaatttg aaacatactt aattgatgaa
     6961 ggattcaaag tgaagaaaaa agattttact tattttacac aggaatcaat tactgaagaa
     7021 ttgatgaagc tatactatga cgcgtacgca aaagatcctg ttgtgaccag gtacattcgc
     7081 aaactattca gtgaaaaacg agaaatcctt agtacgacta agctgaccta tagactgctc
     7141 ctttggtccg cagataaaaa aagaaccttt cacgactcgt gaagggttct ttttatgatt
     7201 ttattatatt ttgtagtaac gaatatcatt ttcgatcttt gtaatcgaaa aaggtacatc
     7261 tgaagtatat tctgcacatt ctccgtgttc aggaagcgtc tcactgaaaa tcgtttcata
     7321 ttcgtcaaca gtgatccgtg ttcgagaatc tagcatctct tgatgagatt gtgcgaagag
     7381 atattcttga tagttttctt ccagataacc tgtaaaaaat tcacttactg caccagaacc
     7441 gtagctgaat agaccgatgc gatctcctgg ctgtagtgat ttcgaatttt ccagtagaga
     7501 agttagcccc aggtataatg aaccagtgta aagattaccg attcgtcggc tgtaacggat
     7561 gctttcttca tagcgagcca taagacgttc ctgattttct tcgtctgttt ggtctaatac
     7621 gctttgcaat gccttttttc ccatcttagt atacggaata tggaaagcaa tcgcctgata
     7681 atcttcgagt cctcgacccg acaattcttt atgtcgattc caaacttttt ggaatgattc
     7741 gatatacgta gaattagata aaggaccatc aacaacagga aattcgctat aatctggacg
     7801 ccagaaatca tagatatctt ctgtcagaaa tacgctgtcg tcttcaatcg ataaaatacg
     7861 cgggttttga gtgatcatca tcgcaacagc accgacacct tgcgtcactt caccaccgct
     7921 tgccaagccg taacgagcaa tatcacttgc tatgactagt acttttcgtt ctggatgatt
     7981 tttgacatat tctttcgcca tgtgcaaagc tgcggtgcca ccgtagcaag cctcttttac
     8041 ttcaaaagaa cgagcaaaag attggatttt cagtaaatgg tggataatta ccgcactcgc
     8101 ttttgaatga tcaatcccgg attctgttcc gacgatgacc atgtcgatta gctcacggtc
     8161 tttttctgtt acgattttgc tggcagcatt tgctcctagt gtaatgatgt cttcatttgc
     8221 acgattgact gccatttggt cttgaccaat ccctatatgg tattttgcag gatcatcccc
     8281 acggctttct gccaactctg tcatatctaa atataaatta ggaataaaaa aggaaagacg
     8341 atcaatccct attttcattt caacacctct ttacttatga taaaaaactt actttactaa
     8401 ggtatcataa aaagattaaa agaaatataa aaaagagaat atttaaggat tgttaaaatt
     8461 taatcttaga aaaaaacaac tggaacaaga gctatttgtt tttacagcaa agaatggtat
     8521 atttttgtag gaggtggctt acttgaaaga agtcgttatg atagatgctg caagaacgcc
     8581 gataggaaag tatcgcggaa gtttaagtcc atttactgca gttgaattag gcacacttgt
     8641 gactaaagga ttattagata aaacaaaact gaaaaaagat aaaatcgatc aagtaatatt
     8701 cggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc agacagattg ctttgaacag
     8761 cggtctccct gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat ctggtatgaa
     8821 agcagtgatc ttagcacgcc agctgatcca attaggtgaa gcagaacttg tgatagccgg
     8881 cggaacagag agtatgtctc aagcacctat gctgaaaccg tatcagtcag aaacaaatga
     8941 atatggtgaa ccaatttcca gtatggtcaa cgacggattg actgacgcat tttcaaatgc
     9001 acatatggga ttaaccgcag agaaggttgc aacacaattt tctgtgagca gagaagaaca
     9061 ggatcgctat gccttgtcgt cccagttgaa agcagcacat gctgtcgaag ccggtgtatt
     9121 ttctgaggag atcatcccag tcaagatttc tgatgaagac gtgttatctg aggatgaagc
     9181 agttcgtgga aatagtacat tggaaaaact gggcacgtta cgtacagtat tctcagaaga
     9241 aggaactgta acagcaggaa atgcttcgcc attgaatgac ggtgcctctg tggtgatcct
     9301 tgcatccaaa gaatacgcag aaaataataa tctgccttat ttagcaacca tcaaagaagt
     9361 agcggaggtc ggtattgacc caagtatcat gggaatcgct ccaatcaaag ccattcaaaa
     9421 attgacagat agatcaggaa tgaatttatc aacaatcgat ctttttgaga tcaatgaagc
     9481 atttgcagct tcttctatcg tagttagcca agaactgcag ttagatgaag aaaaggtcaa
     9541 tatatatgga ggagcaatcg cattaggcca tccaattggt gcaagcggtg cacgtatctt
     9601 gactacatta gcttacggat tgcttcgtga acagaagaga tacggtattg cttcattatg
     9661 tattggtgga ggtttaggct tggcagtctt gttagaagcg aatatggagc aaacacataa
     9721 ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct gaacggcgca gtcaattaat
     9781 agaaaaaaat gtattgacac aagaaactgc gttgattttt caggaacaaa cactttctga
     9841 agagctctcc gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc caatgggcat
     9901 cgcgcagaat tttcaaatca atggtaagaa aaaatggatt ccgatggcaa cagaggaacc
     9961 atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct gcgcagaaac
    10021 acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt atcaagcagt
    10081 gatcaatgca gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg aaagttatcc
    10141 ttcaatcgtg aaacgcgggg gcggtgttca agatatctct actcgggaat tcatggggag
    10201 tttccatgca tacttatcta ttgatttttt agtggatgta aaagatgcaa tgggcgctaa
    10261 catgatcaac tcgattcttg agagtgtggc aaacaagtta cgggagtggt tccctgaaga
    10321 agagattcta ttcagtattt taagtaattt tgctacagaa tcattggctt ctgcctgttg
    10381 tgaaatccca ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa ttgccaaaaa
    10441 aatccaacag gcaggggaat atgcgaagct tgacccttac cgtgcagcaa cgcacaacaa
    10501 agggatcatg aatggtatag aagctgtagt ggcagctaca ggaaatgata cacgtgcggt
    10561 tagcgcttcg attcacgcct acgcggcaag aaatggatta taccaaggat taacagactg
    10621 gcagatcaaa ggggataaac tagttggaaa actaacggtg cctttggctg ttgccacagt
    10681 aggcggagct tccaatatcc tgccaaaagc aaaagcatct ttagctatgt tagatattga
    10741 ttcagccaag gaactagcac aagtaattgc tgcagtaggg ttagctcaaa acctggctgc
    10801 tttgcgtgca ttagttacag aaggaatcca aaaaggccac atgggacttc aagctcgttc
    10861 attagcgatc tcaattgggg ctattggtga ggaaatcgag caagttgcga aaaaattacg
    10921 tgaagcagaa aagatgaacc agcagacagc tatacaaata ttggaaaaaa tccgggaaaa
    10981 ataaaaaagc aatatcttta gaagaataaa gcaagattta cagttatttt atttactgca
    11041 aatcttgctt ttttctctat cagaaaatat gtttattatt agaatttttt atagttaagg
    11101 ttaggcatct taagaaatca ggcaactcca agatatcttc aggtagaaga gaagtcgcct
    11161 aacattgtag gatcataggt gatttgttgg acaggtcaag ctgatatttg agcctgatgt
    11221 tagaaggaat ttctttttac ttgctgtaga aattattata tgactaaaaa ggctgggatt
    11281 ttaaatccca gccttttatg acaaatagaa tttttatcga tcagtaatcg ctgtcgctgc
    11341 aaattgttcg atcaaaggaa tcaaagtcaa tttgactttg tatttccgtt cgagtgcttt
    11401 ttcgataacg aactcatttt cagtagtcat agaaatagtt gctaatttac gaggttcatc
    11461 agcatttttt tcattaataa ctacttcatg ttcattttgt tctgtgtaaa atttaggata
    11521 ctcagcaacg gcttttgaaa aatcagcaaa acttaaagcg gcattcgtat cagttgagcg
    11581 tgtattttca gttgtgtccg cttttggttt ttccgttttt tcttctggca cactgactgt
    11641 ttcttctgta actggtgtcg caacagcttg cgcagttggt tcatcgtttt tcaatcgttg
    11701 atcattccag taagacacga tttcaataag aagattgtca cggaatcgtt cgaaaatccc
    11761 gttttctcct gaacgaattc tctttaatac agtattgaag ttgtcatcga ttggcacgtt
    11821 gatccgtgta tcttcatttg ttttcccagc taatccgatc gttaagattt gacggttttt
    11881 gggatcaagc atgacttgta acggcaaacc atagcgtcgc aagctctctt cttctagaga
    11941 acggaataat tgtcctctta caccatccaa tgaaggacgg aattgattta ataatttttg
    12001 tttctgtttt ttcttcatat gttctctcct tcgctcatac cctttcgatt ataagaaaaa
    12061 aacagccaaa tgaaaaggag taatgcaaaa aaacttgcgt atacgcaaat tacaacagaa
    12121 aaatttgcgt attttcccgt tttttgaaag aaaaataaaa aatggaacgg attatgtata
    12181 tatacataat ccgttccatt ctttctattt tttcggtatc aaggcaaaaa caggtagttc
    12241 aggtgaataa atgacttcat gttcgtgcca gggtttgacc attagcgaat gatcgaaccg
    12301 ttttttcagt tcttcgatga tttgatcatg tgtccagttt cttgcaggaa aatgttggat
    12361 tttctctgca tcgatcaaaa agacaaaatc ggaatgaaaa gtctgggtaa cttcttccga
    12421 gagagaattc aagcgttcga ttggatccat aaaatcacct ttcgacttaa aattgggtag
    12481 tatctgggtc ttttgctttg ccgactacac catctaactg atcgatttgt ttcatttctt
    12541 cttctgttaa ttcaaaatca aataattcag tattttcttt gattctgctt ggtgtcactg
    12601 acttaggaag aggaaggaat tcgtgttgca gcgaccaacg caaagcaact tgtgcaatcg
    12661 tcttgtcatg tgcttcagcg atttgtttca tttcaggcac atcgaagatt ttaccagtgc
    12721 ctaatggact atatgcttcg aggatcatat catgtttttt tgcatactca actacagttg
    12781 gctgtaattc gcctggtgca aggaaaactt gattgaccat cggcataatt tttgctgttt
    12841 ttgctagtgt atctagatgg tggggtaaga aattgctcac accgattgct ttgattttac
    12901 ctgcttcata cagttcttca aatgctcgcc atgtatctgc atttgcttgt tcccaattat
    12961 cacgaaaagc aacagggttt ggccagtgga tcaaaaacag atctaaataa tctgtttgga
    13021 gcttcttcaa tgattcttca aaagaagaca tcacaagttc atagctgtgg tttgcattcc
    13081 ataattttgt tgtcaagaat atctcttctc taggaattcc actttcctta atcgcttggc
    13141 cgacactttc ttcattcttg tatccttggg cagtatcgat atggcgataa cctgcagcca
    13201 gcgcttcttt aacagaggaa acagcgacat ccccatcagg tgtttgccat gtgccgaatc
    13261 ctactaccgg gatttcgtaa ccatttaata gacgataagt gcttgttaat gaattcatac
    13321 tttcagctcc ttttttattc gttcaactat aagtgtagcg catttttcga aaaaaagaat
    13381 ccattttgtt tctactcttg aaatttttgt ttaaaagaaa atgattttga ttctggtatt
    13441 tttgattttt tttccgtaaa ctaagacaga agggaatgag aaaatggata tctatttaaa
    13501 aaaagcaatt ctgcatatta ttgacagaga aactggttct ccagtctttt cacaaaaaga
    13561 gctggatctg acaaaagaat atattcgtga ttttcttcaa aagaagatcc aaaaaatctc
    13621 ttctgctcaa acaaaaactg gacaactagc agaagaaagc atgatcgttg aatcacttaa
    13681 aatcgctcaa gaaaattttg tggaagcaag tgaagcaatc gttcagcggt ggttcgatat
    13741 ctaccaagaa agtgaagaag cacctagtgc agatgtattt gttgttttgt atgaattaga
    13801 tacagtgatg tacttatctt tattgaaagt caattatcgt gaggcttaca ctcactttgt
    13861 tgaagcagac gaagcaggga tcgacaaccg cttgattttg aatcgagcaa ttctaggggc
    13921 gaaaacacaa aaagcagatg aagcattcgc tgtgaatttg actgattgga cctacgaatt
    13981 aattgaaaaa aaatatgagt tttctggcaa aaaagagctt tatttttctg gaagagtgat
    14041 cgaaagtgtt cctgcacctt ctttagaaga aaatgtaaaa gtgatccaaa aagtagccaa
    14101 gcatcttgga aaaaaatttg agacagaaga atttgatatc atggctgatt tgaaagaagc
    14161 tgtctatgat acgatagaag aaaaaggtcg gttggatcat gagatgattg cggaaaaagt
    14221 cttcaaggaa aacatcacgg caaaaatggc ttttaaagaa gaagtacaag agcaaggatt
    14281 tgtcccagaa gcacctcctg taaaagaagt acaagaaatt tctgagaaaa aatttggaaa
    14341 acaaaaattc aagctatcaa acgggatcga actgatcgta ccagtagatg tatatcgcaa
    14401 cccagatttg attgaatttg tgaataatcc ggatggtacg atatccgtga tgatcaaaaa
    14461 tgtggatgaa gtattgaatc gtttataaaa atgactagaa cattaggtgt tcaagtacca
    14521 gtgatcagtt aaatgaattg tgtcaagaga aggatcggaa aagacgaata taacaaaaaa
    14581 agagcgtgag acaaaagtaa
//
LOCUS       DAZKCZ010000064        13985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000064 DAZKCZ010000000
VERSION     DAZKCZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.064"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(216..2213)
                     /gene="tkt"
                     /locus_tag="ACG8KN_001753"
     CDS             complement(216..2213)
                     /gene="tkt"
                     /locus_tag="ACG8KN_001753"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9188809.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2315..2566)
                     /locus_tag="ACG8KN_001754"
     CDS             complement(2315..2566)
                     /locus_tag="ACG8KN_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFP9188810.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2713..3336
                     /gene="lexA"
                     /locus_tag="ACG8KN_001755"
     CDS             2713..3336
                     /gene="lexA"
                     /locus_tag="ACG8KN_001755"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9188811.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3516..3734
                     /locus_tag="ACG8KN_001756"
     CDS             3516..3734
                     /locus_tag="ACG8KN_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188812.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4023..4535)
                     /locus_tag="ACG8KN_001757"
     CDS             complement(4023..4535)
                     /locus_tag="ACG8KN_001757"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9188813.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4549..6846)
                     /gene="recJ"
                     /locus_tag="ACG8KN_001758"
     CDS             complement(4549..6846)
                     /gene="recJ"
                     /locus_tag="ACG8KN_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9188814.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6988..7431)
                     /locus_tag="ACG8KN_001759"
     CDS             complement(6988..7431)
                     /locus_tag="ACG8KN_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFP9188815.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7432..8220)
                     /locus_tag="ACG8KN_001760"
     CDS             complement(7432..8220)
                     /locus_tag="ACG8KN_001760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9188816.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8217..9158)
                     /gene="rnz"
                     /locus_tag="ACG8KN_001761"
     CDS             complement(8217..9158)
                     /gene="rnz"
                     /locus_tag="ACG8KN_001761"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFP9188817.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9335..9922)
                     /locus_tag="ACG8KN_001762"
     CDS             complement(9335..9922)
                     /locus_tag="ACG8KN_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFP9188818.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10143..11456)
                     /gene="obgE"
                     /locus_tag="ACG8KN_001763"
     CDS             complement(10143..11456)
                     /gene="obgE"
                     /locus_tag="ACG8KN_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFP9188819.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11753..12763
                     /gene="gap"
                     /locus_tag="ACG8KN_001764"
     CDS             11753..12763
                     /gene="gap"
                     /locus_tag="ACG8KN_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9188820.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12848..13783)
                     /locus_tag="ACG8KN_001765"
     CDS             complement(12848..13783)
                     /locus_tag="ACG8KN_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9188821.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3878 a         3119 c         2463 g         4525 t
ORIGIN      
        1 ttttgagtta cgtaaatgga aagttttgtc tcgcttaatt tgacaaataa tattctcaag
       61 aaacacaaac taatccgaag aattctttgt tttgtcattt aaatgtaaca tagccgaaaa
      121 cgaaaagtaa tattgattta ttcttctaac acataaagaa cagctgtctc caacttgcag
      181 acagctgttc ttatcagtca aacaattgct tggatctatg atagttgatt gtacgtattc
      241 acgacatttt ctactgtaaa tccaaattct tctaaaattt tagtgccagg ggcagatgca
      301 ccgaaatgat cgattgtaat cgttgcgccc tctgttccta cgtagcgttc ccatccaaat
      361 gatgcagcag cttcgatagc cacacgtttc ttcactgatt taggaagtac actttcttta
      421 tattcagaag attgtttttc aaagaggtca aagcttggca tagaaacaac agaaacatct
      481 ttgccttttt cagccaattc tttttgagct ttcacagcca aatcaacttc agaacctgtt
      541 gcaatcaaaa taccttctgg tgtttctcct tgtgatggag aaagaacata cgcgcctttt
      601 ttcaccattt catctgctac ttcttttgta gaaggcaata ctggaaggtt ttgacgactc
      661 aatacaagga tagtaggcgc atctgttgtc tccatcgcta ctttccaagc agcacgtgtt
      721 tcatttccgt ctgccggacg aatcacttga acaccaggca tgcagcgaac agatgctagt
      781 tgttcaatcg gttcgtgcgt tggtccgtct tctccgacag caaccgagtc atgtgttaag
      841 acgtaaacaa caggagtatg ttggattgct gctaaacgaa ctgctggtct tagataatcg
      901 acaaagacaa agaatgttcc accgtagata cgagttccgc catgaagttg gataccattc
      961 atagccgaag ccattgcaaa ttcgcggaca ccgaaccaga tgttgcgtcc ttcataatgt
     1021 tcaggagtga aatctttatc tgcagccacc attgtattat tggaacctga caagtccgct
     1081 gaaccacccc agaagcttgg tatagctttc gataattctt ggatgacttc tttacttgaa
     1141 acacggcttg cttgactgct tccaacttca taagttggca attctgcatc ccagttttct
     1201 ggtagtttgc catcaaaagc atcttcaaat tgttgtgcta attcaggata agctttttta
     1261 taattggcaa acatttcatt ccatgcatct tcagcttttt gtccttcttc tatcatcgtt
     1321 tgatggaagc gggcagctac ttcttctgga acagtgaaat ctggatactc ccaaccataa
     1381 acttctttag ccatcttgat gccatcttca ccaagaggag caccatgaac agcagatgtt
     1441 ccttctttag gagcaccata gccgatgact gtttttactt cgatcaatgt tggtttgtct
     1501 gtttcagctt ttgcttcttc aatcgcttta gagattgctt ctaggtcatt tccgtctttc
     1561 actaagatat gttgccagcc atatgcttca tagcgagcac caacgttttc tgtgaaagct
     1621 tttgaagttg gaccgtctag tgaaatatcg tttgaatcgt ataaaacgat caatttccct
     1681 agtttcatat gacctgccat cgaacttgct tcttgagata ccccttccat caagtctccg
     1741 tcaccgcata ttgcatacgt atagtgatcc atcacattaa agttttcttt gttatacgtt
     1801 gctgcaaggt gcgcttctgc cattgccata ccgacagcca ttgcaatccc ttgtcctaat
     1861 ggccctgtag tggcttctac accatctgta tgatgtactt caggatgtcc tggtgtttta
     1921 gaatcccatt gacggaattg tttcaaatca tcgattgtca cttgatagcc agctaaatga
     1981 agcaagctat acaacattgc tgacccatgt cctgccgaaa gaacgaaacg gtcgcggtca
     2041 gcccagtttt ttgatgtcgt tggatttact ttcaggtgtt ttgtccataa cgcatatgcc
     2101 ataggtgctg cgcccatcgg caatccagga tgtcctgagt ttgctttttg gactgcttca
     2161 atacttaatg tacgaattgt gttaacgcca agttggtcaa ttttgtcaaa caaaataacc
     2221 cctcctatta atttaggttt tttttacctt tatttcttac actttcattc tataagatgt
     2281 cacacgaaaa agcaatcttt ttcagtattt tttactaaaa gttgtcattt cggttgtgta
     2341 agcctttttt cttttggatt tcttttaatt tttccggggt cacgtcattg ccctcaggat
     2401 cgacgacttt cattccttcg atatgatgac gcataccgga acgaaaagct tccaaatact
     2461 ctttgcgcaa gttgatttgt tcttgttctt cggcttttga gagaccattt tccttctttt
     2521 tacgtgcaag ttcattgatc cgctctattt tttcaggtga cagcatgctc agccctcctt
     2581 ttactctcct tcatattata taaaaactga aaaaaaaaca actagataaa ctaaataaaa
     2641 tacgaacatt cgtttgattt agcgcttttt atatggtact atttatttat caagtgaaag
     2701 aaggtgtacc ttgtggcccg acaaactgaa tcaagacaaa tcgaagtctt acgatatatc
     2761 catgaacaag taacccaaaa aggatacccg ccgactgtcc gtgaaatcgg ggaagctgtt
     2821 cagctttctt ctacttctac tgttcacggt catcttgcac ggcttgaaaa aaaagggttt
     2881 atccaaagag atccgacgaa acctcgtgca atcgagttga caaaacaagg tttagaaaag
     2941 attgggattc gtccaacaac tattccagta cttggcgtcg taacagctgg agaacccatt
     3001 ttagcagtag aggatgcttc agacttcttt ccaatccctc ctaatctttc ttccgaagaa
     3061 ggcagtctct ttatgctgac aattcgtgga gatagtatga tcaatgctgg gatattggat
     3121 ggtgacaacg tgatcgttcg gaagcaagaa actgctcaaa atggcgatat tgtcattgca
     3181 atgactgccg aggacgaagc gacttgcaaa cgattctata aagaaaaaaa ctttttccgt
     3241 ctgcagcctg aaaacgactt gctcgaacca atcatcttag atcaagtatc tatattagga
     3301 cgagtagtag gactttatcg tagtcatatc tattaattgc tactaattaa aagaaaactt
     3361 tttaaaaaga gtttgagacg ttggtctcaa actctttttt cgcccgggtt cttcttcttt
     3421 caaattttta tcacttccct ctttacaaac cgaacaaata ttcgtatact aaatatacga
     3481 acaattgttc gcataaatta aagaggtgag aattcatgaa atcaatgaga cgtcttggct
     3541 tagtattaac tgttttgttt ttaggtatta ttattggtaa tttcggcata cgtccagctt
     3601 tacttgtgtt tgttccgtta tttactttgt ggttcatgtt gtgggatgaa aagaagtacc
     3661 gtcaatctga gcggcgcaga attcatgaag atcatttcta tcatgagccc tactacagat
     3721 ccaggcaatc ataataagga ttgactgcta attattcaaa agacaagaag ttaaactttt
     3781 acgggggtgt gacaaaaatc ggtcacatcc tttttttcga ataaacggtg ttctaaagcc
     3841 agtttttcgg tattaagtca aattgcggaa aaagtgattt tttaaacttc tactggtctt
     3901 tgcttgtttc catttataaa aaaatagcga ccctcaaaag tcagatagag aatctgactt
     3961 ttgagggtcg ctttaaaatc gcttctttaa gaataacatc aagaatagct tcttttttct
     4021 gcttaatatt ccattaatgt aatgatgtca tatcctttga ttttgtcgcg tccatggaga
     4081 tccatcaatt cgatcaaaaa tgcgcagcct acgactacac cgcctaattg ttcgatcagt
     4141 tcgatcgtcg ctttgattgt tcctccagta gctagcaaat cgtcacaaat cagcacacgt
     4201 tgacctggtt taatggcatc cttatgcaat gtcaatgtat ctgttccata ttctaatcca
     4261 taagtcactt cgatggtttc tcgaggtaac ttcccttttt tgcggactgg ggcaaaaccg
     4321 actcctaact cataagcaac aggacatcct acgatgaatc cacgggcttc tggcccaact
     4381 accatatcaa ttcgtttttc ttttgcataa tttacgattt gtttagtagc ttcccgataa
     4441 gcgtcaccat ctgccatcag tggggaaata tctcgaaaca caattccttt tgacggatag
     4501 tcgggaatac tggcaatata atcttttaaa tccactcttt gtttcctcct aggttgttaa
     4561 ccatttcttt atggtaggaa tatcgctcaa aagcaaaaat tcctcaactt tgattttatt
     4621 gagccgtcgc tggtataatc tgctctcagt caaaggatga ctttctgggc tagcgacttt
     4681 ttgtaaaaca ccgttctcta ttgtaacaaa tttcaattca aaaaacacct gtatcataaa
     4741 aattaataat ttttcaggga tacgcaaata ttgagctatt gcttttattt tatgacgtat
     4801 atcaattcgt tcttgagaat ggatcaattt aaacaatttt gcgtactgtt ctcttgtgcc
     4861 taccccattc aaataggcct catcaggtga aatccccatc agataaacac gattgaatga
     4921 ataagaccga aaaacttctt tcagatccgc taaatcatct ggacaatcca taacaactag
     4981 agagtttggt tttctttttt caattattgc ctttacagaa tctagtcctt cgaaaacaat
     5041 cgcagcttcc tggataatct caggcacgaa tttcaacgtc gaagaatcaa atgctaaata
     5101 aaggctacct tccgacgttc ggctctgttc acggaaacgt tttgcccgcc aatcaaatac
     5161 ttgcagacca cccacagaaa aatcagatac catcagctgc ggtttcttac gcccgttcca
     5221 ttcattgatc gaaagctttc ctaccacatc tacctgatta ttcagcagtt cttcttcttg
     5281 agtaccaaaa ccaaaagcga ccgcatcaag ctgactcgat gcatcggaaa ggacaaattt
     5341 cagatgctgt tggtttgctc cgattctttt gatattttct gcttggactt gtcgaaataa
     5401 aaactgaggc aaaggattat ccgtaccaaa tggcgccaat agtttcagct catcaattcg
     5461 ctctactgtt acttcatttg gcagcaatac ttcatcaatt ctcaactcag gccctctcat
     5521 caagtcaatt tgatgatcga ctatatattg gttcatcttc tcctgtagta tagtgacatt
     5581 ttcacttggc atcgttaaac cgactgctgc atgatgtcct ccgaagtaag taaataaatc
     5641 tcgcatctgg tcaagcattt caaaaagatt caaggcttca atactacgtc cagatccttt
     5701 agctgatcca tcttctttta aagtaaggac aagcgttggt ttacccgttt cattcattat
     5761 tttcccagca acaatgccca aaacgccttc atgccatcct cggtttacca atagatggat
     5821 atgattctcc ttattgacca tcgctaatgc ttcttgtgtt atctgctcta cgattgcttt
     5881 tctttcttca ttgatctcat tcaatttttt tgcttgaacg gatgcttcct cttcatcaaa
     5941 cgtcgcaagt aacgagacgg caggacgtgg atcgcccatc cgaccgattg catttagtct
     6001 gggagcaatc gaaaaaccga tcgttgtttc gtctgcgtcc ctcattttta cgccgctttc
     6061 ttcaaatagc gcctgaagtc ctatccgttc tgaatgatga atggcatcga ttcctaatga
     6121 aacaaggatt cgattctcat cagtcaatga aactaaatcg gcgatcgttc ctatcgctac
     6181 taaatccaaa aattctgcag gcggttcttc caataatgca gaagctactt tgaatgctac
     6241 tccgactcct gccaaatctg gaaatggata gtgccccgct ggatgacgag ggtgaacaat
     6301 cgcataagcg tctggtaaat tctcaggtag ttcgtgatga tctgtaatga tcacgtctac
     6361 accagcttgc tgagcgtatg ctaccgcatc atttcctgct accccgttat caacagtgat
     6421 gatcaactgg attccttctt cgatcttttc ttggtatacc gattggtttg gtccataccc
     6481 atgctcaaat cgatttggca ggaatacttc tacttctgca ccaagtaatt ccagtgtttc
     6541 tttcattacc gttgcactgg tgatcccatc tgcgtcataa tcaccgtaaa cgaggatttt
     6601 ttctccactg attacagctt cttggattcg gctgattgct ttatccatat cgtgtatcag
     6661 atacggatca tgtaattctt gttcctttgg atgtaaaaat cggttgatat ccttttcctc
     6721 atggtaacct ctttgccaca gtaacttacc gacaaatgga gataatttca gttcacggat
     6781 catttggaga aaggattcag aaggctctgt ctcgcttaac ttccaatcaa aatttgcttg
     6841 tctcactttc tcactcctaa cttagttatt atagcaaagg aaatcagcaa aaaaaagcag
     6901 aaagaaagaa taaaaacaga tcagatccgc tagacagatg ttcagcagga cctgatcttt
     6961 tgtacgacca tttatgaaat atccctatta gtcaaaataa ttgaagcttt gtttgggcga
     7021 ttgagttttt tgttcttccg gcgctttcaa ttgactgatt tgtgcggttt tcgcttgtaa
     7081 ctccgcctga taagcagctt ccaactccgc acgttcgttt ctaaactcgc gttgctgttc
     7141 ttcttttgct tcttcaattt tcttttgaag ttctgtttga tattctgaca tttcctgagt
     7201 cagctgtttg atttgctttt tttgctgcca tagtgttgcc gaagcagtca aaaatacaat
     7261 cagtgcacca atcagcgcag aaccgatgat cactaagatc aatggcgcag aaaaggaagt
     7321 gaagccaaaa cttacaggaa catttttatt gttcaataca gcaaaaagaa caataatcaa
     7381 aactaaaaca aatccaagaa tcacattgct ttgctttttc aattccttca cctacttttt
     7441 gttaaaaatt ccgctggcta aataatcacc aatcgttgga aacagcgtat aaaattttga
     7501 tgcaacttct aaaataaacg gttgattgat ttcccgtttt ggatgtctca tattgcggac
     7561 gatcattttt gccaacttgg caggttctaa aacgatttgt ccgacttttt ccaaatagct
     7621 tccgcttgga tcagccttat cgaagaaatt tgtctcgatt ggacctggat tcactgtcgt
     7681 aacagctacg ccaagtggtt tcaattctaa tcgcaaagcg ttggagaagc caaggactgc
     7741 aaatttcgtg gcagaataaa ccgttgattt tgcagttgcc atttttccag ccatagaggc
     7801 gatattaatg atatgacctt gtcttctttc tgccatatcg atcgcaaatt tttgtgtcaa
     7861 aaccatcatt cctaagatgt tgacttcaaa catatcgtat gcttttccaa ggtcgaatgt
     7921 aaggaagtct tcgaaaatcc caaaacccgc attgtttacc agaatatcga cgcgcccgac
     7981 ttcttccatc accttttcat ataattcgtc cacgctttca ggattgctga cgtctacctt
     8041 gaaagcataa gcctctttgc cgctcaagcg tctgcactcg tctctgacac cttctacaaa
     8101 tgccattctt cgtgcgcaac ttataacgat tgctccttgc tttgctgctt cataacagat
     8161 ttgtgcgccc agaccgcctg aactacctgt tactaaaaca actttatctt ctaaattcat
     8221 gcctcttcct cctcgcgaaa tggaatctca atgctatcaa aatctttcat aatcgtcgtg
     8281 ttttcaaaca cttcttgtgc ttctttttca agctctaaag ctgcttttcc taaatatctc
     8341 gcactgatat gagtaagtag cagccgtttt gcctgcgctt cttttgcaac ttcagcagct
     8401 tgatgagtcg tggaatggaa atagttgtgc gccattcttg cttcatcttt attaaatgtg
     8461 ctttcatgta ccagaatatc gctattttcc gcaagcacaa cggaattatg tgtttttctc
     8521 gtatcgccta aaatagtcac gattcgtcct tttttatctg gacctacgaa atcttttcca
     8581 ttgatcgtcc gcccatcttc tagttggatc gtttcacctt gcttcagttt gccgtacagt
     8641 ggtccagctg gaatacctaa ttcttttaga cgatccacct gcaattctcc tttatgatca
     8701 tgttcaacca cacgataacc aaaactgtcg atcccatgat ttagtttttt tgtataaaca
     8761 gaaaattgct gatcagtaaa aatcggggct gtttcattta gctcaatgaa tttcagcgga
     8821 taacttaaac gcgtttgcga aatacctaaa gagactttta tgtattcctc tatccctttt
     8881 ggcccgtaaa tctctaatgg cgtatcccca ccttgaaatg accggctgct gatgagcccg
     8941 ggaagtccaa aaatatgatc gccgtgtaaa tgtgtaatga aaattttacc gatttttctc
     9001 ggacgaatag tcgttcgcaa aatctgcatt tgcgtgcctt cgccacagtc gaacaaccaa
     9061 acttcattgc gctcgtctaa aagtttcaat gctatacttg tcacatttct atgttttgca
     9121 ggaacacctg cacctgttcc taaaaattgt aattccataa tctacctgac tttctttaag
     9181 ttcctcttta attttagggt aaaaagaagg gataatcaac cgtaagtcga gaatgggttc
     9241 aaattaaaaa taacgttcta aagcgatagc gttttctttg aaaattacta taaaaacagc
     9301 agcgaacccg tttgagatcc tctgctgttt tttattaatt tgaagatcca gaactttcat
     9361 tgtcgttgga agtttgcgga tgtttgtcct tccaagtctt ttgtgcagct tctttcgcat
     9421 gattttcagc ttctttcgca tcatgcacat ctttttcgac atcttcataa tccaagcgag
     9481 taatttctcc gcctaattcc atcattacca attgattcag tgggcgattg tcctcttcat
     9541 cagcaatcaa gatcagtcct gtagctcctt caccgatttt atttcctaca aagtcgaaca
     9601 cggtctgggc atcttttatt tctttggcat cttgtgtcgc accgatcatt cctccggcaa
     9661 accatcctaa taaaatccct agcggaccac ctaatacacc aaccaacatt ccgattaatc
     9721 cacccgtaga agatttgtta ttaccggtaa agtcgataaa atcttctatt ttgaattgat
     9781 gtgtcccatt aggatcatgg ctgacaactg ccatctgttc tcctttaatc gaacgaacag
     9841 catgtaactt tttgatttct gaaaatgcct gatatgcctg gctttctact tgaaaattta
     9901 aaatgattac tcgtttgctc attaaaaacg cccctttcat accttcttgt tatttatttt
     9961 acctttaatt gtaaaataag aaaagaaaag cactcttttt ttccaataaa atagggctgt
    10021 gacaaaagtc gtatcacagc tcctttttcc gaataaacgg cgttcttgaa gctgatcact
    10081 tctgtcacaa cctcccttaa atctgaatct atattttttg gtatttgttt ttatttgctt
    10141 gcctattcga caaattcaaa ttcaaactca ccgatacgta caaggtcgcc atctttcgca
    10201 ccgcgtgccc gaagagcttc atccacaccc ataccacgta attgacgagc aaaacgcatc
    10261 actgtttcat catgatcgaa attcgtcatt tggaataatt tttcgatctt ctcaccagat
    10321 aagatccacg ttgcatctga atcacgatcg atttggaact caggaccttc tggattgaat
    10381 ccatagtgta cagtttcttc ttccagttct tcttcgtaca atgggaattc tggcgttact
    10441 tctaacaaat ctgctgtagc attcaataac gcgtccaatc cttgtcgagt cactccagaa
    10501 attggaaata ctgggatgtc atcagcaaat tcatcttctt tttccttgtt cagctgttct
    10561 ttgaatttca ctagattttc cggtgcatcc ggcatatcca ttttgtttgc cacgatgatt
    10621 tgcggacgct ctagcaagcg taagttataa gtagataatt ctttgttgat ggcaagatag
    10681 tcttcataag gatctcgtcc ttccatccca ctcatatcga tcacatgtaa aattactcgt
    10741 gttctttcga tatgacgaag gaattgtgta cccagcccaa caccttgcga agctccttca
    10801 atcaagcctg gcaagtctgc tgcggcaaaa cttctgccat cacttgtagt aaccattcct
    10861 agattaggta cgagcgtcgt gaaatgataa gcgccgattt ttggacgagc agatgaaata
    10921 acggatagta aagtcgattt cccgacagat ggaaaaccta caagtccaac gtctgccaat
    10981 actttcaatt ctagttcgat ttttcgctct tgtcctggtt caccgttttc agcaatttcc
    11041 ggtgcaggat tccgaggaga ggcaaaacgg atattccctc gtccaccgcg tccacctttt
    11101 gccacaacta gtgtttgacc attttcaatt aaatctccta aaagagcacc agtctccgca
    11161 tctcggacag tggttccttg tggtacttta acataggtat cttctgaacc acgaccgtgc
    11221 atccctttac tcatcccatt ttcacctggc tgcgctttga aatggcgatt gaaccgaaaa
    11281 tccatcaatg tacgtaaccc ttcatcgacg atcaagatga catcgccgcc acgaccaccg
    11341 tctccgccag ccggtccgcc gtcaggaacg tatttttcgc gacgaaatgc aaccattccg
    11401 tctccgccct tgccagcttt cacatcgatc gttacttgat ctaaaaacat ggacataatt
    11461 aagtcctcct attattcatt tatttatttg actgcctgaa tttccacaat aaacaaaagt
    11521 cttacttatt ttatcggtga aatctgattt tgtctatcat ttcagcaaaa agattgttta
    11581 ccttcttctt tttttatcat ttggtaaggt ttccaacctt ttttctgcta ccaagtataa
    11641 ttttttgaca aaatattctt tgcgtagcag aataacagtg tattaattgt attaattgta
    11701 ttaattgagg atgccgtttc acagcacccc taataaagga ggaacttttg atatgacagt
    11761 aaaagtaggt attaacggat ttgggcgtat tggccgtctg gctttccgcc gcatcaaaga
    11821 agtctcagat gatattgaag tagtagcaat caacgactta acaagtccaa ctatgttagc
    11881 acaattattg caatttgact ctactcatgg tacttatcct ggcacagtta ctgcaacaga
    11941 agactcaatc gtagtggatg gcgaagcaac tcgtgtttat gcagaacccg acgcaagcaa
    12001 aatcccttgg gcaaaagaaa acggtgtaga tatcgtacta gaatgtactg gtttttatac
    12061 atctgaagaa aaagcaaaag cgcatttaga tgctggtgtc aaacgagtag ttatttctgc
    12121 cccagctggt gctatgaaaa ccatcgtcta taacgtcaat gatgatacat tagatgaaaa
    12181 cgacaaaatc atctcagcag gttcatgtac aacaaactgt ttagcaccta tggcttattt
    12241 cttaaataat gaatttggga tcgaagttgg tacaatgaca acagttcatg cctacacttc
    12301 cacacaaatg ctcttagatg gacctgtaaa aggcggtaat ttgcgtgctg ctcgttcagc
    12361 tgctgacaat actatcccac attctactgg tgctgcgaaa gcaatcggct tagttatccc
    12421 tgagctacaa ggaaaattac aaggacacgc acaacgtgtt ccagtcgttg atggttcatt
    12481 aactgaattg gtttcagtat tgaaaacaaa agtaactgct gatcaagtaa acgaagcaat
    12541 caaaaaacac acagttgaca atccttcatt cgggtatgat gaccgtcaaa tcgtttctag
    12601 cgatgtcatt ggaacaactc aaggttcgat ctttgaccca acgcaaacag aagtaacaac
    12661 tgctggtgat ttccaacttg ttaaaactgt tgcatggtat gacaatgagt atggatttac
    12721 ttgccaaatg attcgcttac tagaaaaatt cgctaacttg taatttgata gatcaatcga
    12781 taaaagaagc ctttgactgg atccagtcaa aggcttcttt cttttcatcc cctactttta
    12841 aactcgttca atgaacatat tggcttaaaa attgttggac tacttgctga tatttttcag
    12901 gatctgtctt gtatgcttcc gcatgctcgg ctccttttac aacatacttt tcttttggtc
    12961 ccttcgttgc acgatagacc ttatctaaca tttcaaatgg tacaaatgta tcttcgtctc
    13021 catgtataaa gagcattggg cgctggtttt tcttcaattg ttccactgca tttgcttcac
    13081 cgaaaaagta gccagcacgg acttttgtca ctagactcgt tacttgcata agcggaaatg
    13141 ctggaagact aaacatatcg tctagctgat aagcaagctc atcatgaacg gaagaataac
    13201 cgcaatcttc aatgattgct ttcacattat ctggtagttt ttctccgcta gtcatcataa
    13261 ctgttgctgc tcccatgctg acaccgtata gaacgatttc ttgcgatttt ccattgtttt
    13321 ctagcacttt gtttatccac tgtacatagt ctttacgctc tggccatcca aatccaatgt
    13381 aatccccttc gctttttcca tgtcctcttg catcaggaac aaggacattg taacccatat
    13441 catgaaacat ctttgcgtaa ttggtcatcg tttcagcgtt tcccatatag ccatgagcca
    13501 caagagctgt tttttcactt ttcgtttcag caggaagata aatcgctgat aatttcagac
    13561 cgtcttttga agtgatggtc caatcgcttc ggttttcttt tgaactaaac catttatgtg
    13621 catccgtttc tttggatgtc tgtgttgtac caggtgtatc ttcgtctaaa aagtctttct
    13681 cagaaggcac aacagcataa ttataaaaat aattccctgc aaacactaac gcaacaatca
    13741 gagctaaagc gacgcctaaa aggattttca tccaaagttt cataaatcat ccatctcatt
    13801 tctctttttt catataacct tgaattagta taatcaaaga atctctacta tgcaatgttc
    13861 atttttataa cccgcgaatt gtaaaattaa gttagaaaaa taaaaaggca tttatggtac
    13921 actcaaattg tatttccgac gaaagaaaac aaaggagtgt aaccataaat gacctacaaa
    13981 catct
//
LOCUS       DAZKCZ010000065        13971 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000065 DAZKCZ010000000
VERSION     DAZKCZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.065"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..204)
                     /locus_tag="ACG8KN_001766"
     CDS             complement(<1..204)
                     /locus_tag="ACG8KN_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9188822.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGW"
     gene            complement(683..1402)
                     /locus_tag="ACG8KN_001767"
     CDS             complement(683..1402)
                     /locus_tag="ACG8KN_001767"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9188823.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1451..3097)
                     /locus_tag="ACG8KN_001768"
     CDS             complement(1451..3097)
                     /locus_tag="ACG8KN_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFP9188824.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3276..3866
                     /locus_tag="ACG8KN_001769"
     CDS             3276..3866
                     /locus_tag="ACG8KN_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9188825.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3958..5463
                     /locus_tag="ACG8KN_001770"
     CDS             3958..5463
                     /locus_tag="ACG8KN_001770"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFP9188826.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5547..6899)
                     /gene="celB"
                     /locus_tag="ACG8KN_001771"
     CDS             complement(5547..6899)
                     /gene="celB"
                     /locus_tag="ACG8KN_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9188827.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6899..7417)
                     /locus_tag="ACG8KN_001772"
     CDS             complement(6899..7417)
                     /locus_tag="ACG8KN_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188828.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7433..7759)
                     /locus_tag="ACG8KN_001773"
     CDS             complement(7433..7759)
                     /locus_tag="ACG8KN_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFP9188829.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7772..9751)
                     /locus_tag="ACG8KN_001774"
     CDS             complement(7772..9751)
                     /locus_tag="ACG8KN_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9188830.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9772..10086)
                     /locus_tag="ACG8KN_001775"
     CDS             complement(9772..10086)
                     /locus_tag="ACG8KN_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFP9188831.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10254..10547
                     /locus_tag="ACG8KN_001776"
     CDS             10254..10547
                     /locus_tag="ACG8KN_001776"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFP9188832.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10625..11737)
                     /locus_tag="ACG8KN_001777"
     CDS             complement(10625..11737)
                     /locus_tag="ACG8KN_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9188833.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11890..12738
                     /locus_tag="ACG8KN_001778"
                     /pseudo
     CDS             11890..12738
                     /locus_tag="ACG8KN_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13091..>13971)
                     /locus_tag="ACG8KN_001779"
     CDS             complement(13091..>13971)
                     /locus_tag="ACG8KN_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9188834.1"
                     /translation="EDGKLAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAV
                     VELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDG
                     LAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQS
                     GEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATP
                     VTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILG
                     VLLVFISIKFRKKRNI"
BASE COUNT         4351 a         2865 c         2391 g         4364 t
ORIGIN      
        1 ccaacccacc caaggacttt ccctcaagct agccaagaac ccatctgctg aaatatgaaa
       61 atttttgaat gctacgacat tcgccattac aggtatgtaa aagaaaaaaa tcatccaaag
      121 catacccggt acagccatca ggacaagtgc tttataacgc catagattca taaaaaaatg
      181 attgaacccc tttcgttttt tcatctaaaa acccctttcg attcattcgc aacgtaatta
      241 tagcgctttc aataatgaga ataaataaac aaagtatagg gaaaatgaaa caaagtatag
      301 gagaggttgt gaaatcagcg ttttggcctg agtattagag aaaaaaacgg gaaaatagtt
      361 cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttat
      421 cagaggttgt gaattcagcg ttttggcctg agtattaggg agatcgtgtc cttactatct
      481 aactctgagt cacaaggaac aatggggact gtaacaaaag tcttgtcaca gtcccttatt
      541 ccgaacaaac ggtgttccag aagtagcttc tacggaaata agccgaaatt tcacaaaaaa
      601 gccatccgaa agtcagacgg acaagtctgc ttttcagatg gcgctacaac tactctgctt
      661 attcttttag ctatccattc aattattttt ctctcacttg ttctagctct tctttggtca
      721 tgggtcgata ttgcccttta ttcaaatctt taggcagcca aaattttccc atacggattc
      781 tttttaaata taagacttct tttcctactg ctttgaccat tcgcttaact tggtgaaact
      841 ttccttcatg caaaatcaga cggataattg aagtttctgt ctcttgatct acttgatcaa
      901 tttggagttt tgccggcaat gtctgctcat cgccatcaat aatcagtcct tctgcaaata
      961 aacggatatc ttcttctgtc attataccgc taacctcagc tagatattct ttctccacat
     1021 gttttttagg tgatagtaac tgatgggcaa acaccccgtc attcgttaaa atcaagaggc
     1081 cctctgtgtc tttatctaat cggccgactg gaaaaagatc ttcccgataa tcctggtctt
     1141 ccaataaatc gattacggtt tgatcatgat tatcctgagt agcagaaatg actcctgcag
     1201 gtttatgcaa aatgtagtaa aaatcttttt gatacgtaat gatttctcct aaataagtaa
     1261 ccgcatcttc gaattcattt acttgcgctt tatcgctttt caccacttgt ccattgactt
     1321 cgatcgttcc ttttttgatt aatcctttga cttctttgcg gctgccaagt cccatatctg
     1381 ctaaaaactt atctaaacgc atgcgtttcc cccatttcaa aagagcatca atctagctct
     1441 tctgattcac ttattttatt tttaatttat gtcgtaaacg cgctattcct ggtcctagta
     1501 gtctttctgc caagcggatt ttcaaagcta aataaccata cactaaagcc cctatacctg
     1561 ctactacaag aatcaacaat agagactgga atttgctatc ttcgtttaaa aaagcaccaa
     1621 aaatcatctt tgtgatccac gaagccaaaa gcataatcaa ggtaagaata aagatcagca
     1681 atgtcctgcg ccagacaaaa cttggtttaa agcgggctac ttcatggatt ctctttaaga
     1741 tcaaggtaca agacacagca aatccgatca ttgtcgccaa taaagggccg tatacttcaa
     1801 ataaacgaat ggctgggtat tgaagaagga gtttcactag aaacccaata accaaataat
     1861 taattgccgc cttgttttca aacattcctt gcagcatgtt ggaaactagc atatatagtc
     1921 ctaggaagag tcctacaaaa cttgcttgga tcagtacatt acttcctaga gaatccggtg
     1981 tataaaataa tgtatttagc ggataggcaa gtaacataac accaaatgtc gccggaaaca
     2041 taaaaaatga aaacaactgt aaatttccgc ttgtcaattt tgccagacct actcgatctt
     2101 tcaacgtaac agcttctgta atcaacggta acccagtaga cgcgatcgat gttgccaatg
     2161 caataactac catcgtcaac ttatctggat tggcactaaa aatcgaaaaa agatccagta
     2221 actgtgcatt ggaatactcg gtggatgcag acatgatctt cataaaagtg aactgatcga
     2281 ctaatttgaa gatcgttacc cctgaaccca caataataaa cggaatcgct tctttaacgg
     2341 tatctaacaa caattccttc gttgcaatat ttattttgtt ttcgctgtag tgaataaacg
     2401 ccgatgtata cgcttgatgt ttcttcaaaa aatacaaaag gacaagaata cttgctagca
     2461 taccgataaa tgccgcgaaa gttgattgcg tgaccgccgt cacatagtca ccatccatga
     2521 ctttcatgat gatgaatgta gccaatagca tataaaaaat acgtgccacc tgttctacaa
     2581 tctgcgacag cgcataaggc atcatttctt gattgccttg caaataccct cgtatcacac
     2641 tcatacaagg aaaaaccaat acagcgatac tcaaagaacg catcgttgga atcagttctt
     2701 ctccgccacc agaagcatga gctagccaag gagaggcgat gtacatgaac aaagcaaaca
     2761 acaggcctaa cccgcccatc atctgtaatg cacggataaa taatcgtcgt gaagtgccat
     2821 attcattcaa tgaattatag cgtgcagttt gttttgcaat cgcagcaggt attcctgctg
     2881 tagagatcag tagaaacaat gcatatatat tgtatcccat attgaataat ccattggcag
     2941 ccttagcatt ttcgcccatc caagcatacc agggaatgat atagacagcg cctagcaatc
     3001 ttgaaatgat attactggcg gtcatccaag cagagcctcg tgccattttt tcttgcggtg
     3061 ttaattgatc taatgctggt tttctttgat tatccatcgg tcagattcca actttctaca
     3121 aaacattact atcattttaa ttggtaaaac agaaactggc aatcgatttt atataacttt
     3181 tataagaaca attgtttttt tgaattcggt ttcgtttttt tggtaaagta aagaaatata
     3241 cagcctttta tggctaacat ttataggaga tatcaatgac acaagaaata aaaaaactac
     3301 ccgttatatt tttaggtctt ttctttatca cagttggatt gaattggttc ttgctccctc
     3361 acgatatagc ttccgccgga gtgggcgcca tcggacattt agttgaaacg gccttttcta
     3421 tccaacgctc agttactgtt tggagcatca atcttatgat gctgcttcta gccgctgtcc
     3481 tattaggaaa atcagtattt tttaagactg tagcgggaag tctgcttttt cctgttgttt
     3541 tagagattgt tccaaaagtg gaaatgatct cctcacattt cttttcactg atttcaggaa
     3601 gtcttttgtt cagtcttggg gtttacacat tgtacacagt cggggcctca aatggcggta
     3661 taacgatacc gccgattatt ttccacaaat tttttcggct tccaatgccg caaggacttt
     3721 tacttaccaa tagtcttatc gtctttttga attattatgt ttttggattg ttggagacac
     3781 tattcgttct cctatctatt acagtcagtt cattgtttat gaaaatactc atccgcttgt
     3841 cccctgtgtc aagaaaattt gaataaagct ggattttgtc atagcggaaa caaacgatat
     3901 attaaaaaga gtgtgctata ataaaccaag aaattgaata aagcgaggga tcaatcaatg
     3961 agcttctctt tagaagacat ccgtcattta ctattaaaag aaaatctatt aaaagaattt
     4021 gtttcaacaa gaggctggca tcttgatgta ccagaaaaag ctgaatttct tcagttaagt
     4081 tatgattcaa gaaaagctga tgcttctact ttgtttttct gtaaaggaat gaatttcaaa
     4141 gaagaatatt tggattcagc aatcgaacag ggtattcaat attatgtatc agaacaacca
     4201 tacgaaaatt cagcagtagg aatcatcgta acggatatcc gtaaagcaat ggcgcttctt
     4261 gctatggctt tttacgacta tccgcaaaat aaattgaaag tgatcggatt cactggaaca
     4321 aaaggtaaaa caactgctgc gtactttaca aaagcaatct tggatcacac aacaaataaa
     4381 aaaacagcat tgctttctac tatgaataca acgttagatg gcaaaacata tttcaaatcc
     4441 catttgacaa cgcctgagtc attagattta tatcgtatga tgtcagaagc agttgaaaat
     4501 ggtatgactc atctagtgat ggaagtttct tcacaagctt acaaaacaca acgcgtatac
     4561 ggcttgactt tagatgtcgg aatcttcttg aatatctcgc ctgaccatat cagtccgatc
     4621 gaacatccga catttgatga ctatttctat tgcaagcgac aattgatctt gaattcaaaa
     4681 acagttgttt taaatcatga aagtgactat tttgatttac ttagggaaac agccgatcta
     4741 ttccagattc caacgatcac ttatggtcgt tcggaagaaa gcaattatca agtgatccgt
     4801 tcagataaag gtactcacgg ttttacttta tcttctcatg aagatcgctt agctatctgc
     4861 gatactgctt atgatatttt gctggctgga ggattcaacc aagaaaatgc agccagtgcg
     4921 atcattgccg cagcactaac aggtgcctcc agtcaagatg cacaagacgg attaaaagaa
     4981 gctcgtgtac caggccgaat ggatcaattg atccaatcca atggagcgca tgtctatgtg
     5041 gactatgcac ataattatct aagcttgaaa acattgctga aatttgccaa aaatgaacat
     5101 ccagatggcc gtgtaattgt tgtattaggc agcccaggaa acaaagcaat ctcccgtcga
     5161 catgattttg gaaaagtgct atcagaaaca gctgacatcg cctttttgac ggcagatgac
     5221 ccagcatttg aagatccgaa aaaaatcgcg gaagaaatca atgaagcaat tactaatcca
     5281 gatttgatcg ttcattatga aatggatcgt cctgaagcga ttcgacgcgc tttagcagaa
     5341 agcgctccgc aagattcagt cgttattgcg ggaaaaggcg tggatcccta tcaaaaaatc
     5401 aatggtgtag atgaacctta tgaaggggat tatgcaatcg tgaagcggtt gattgaagaa
     5461 taatggtatt tatatactag tggtttccca tttctaagaa aaaaagggac tgtgattttt
     5521 tgtcacagtt ccttttttct tagaaatcat tttaactttt cactagctaa ttctgctgcc
     5581 ttttctcttt cttcatttaa taatttatta tcatagaatt tgataaatgg gaaccagata
     5641 atgaatgcga tgattgcaca aatgactgct aacacagcag ctttccaatc accaccacta
     5701 ccaacaaatg ctccgatccc gactggtgaa ggccacggca tctgtgcaag catcggtttg
     5761 acgatttcca gccgaatggc aaaataagcg agtgtagcag aagccatcgg agctaagaaa
     5821 aacggaaccg ctaaataggg attgtagacg attggcatcc cgaaaatgat tggttcattg
     5881 atattgaata atcctggtac aagtgaggct tttcctaaca ctttcagctg atcagatttc
     5941 gcgaggaatg caataaagat gatcaatcct aaagtcgcac ctgatccacc aacggttaca
     6001 taagaattgt tgaattctcc tgccaacgga taatttgcgc cttgtgcgtt agctgccata
     6061 ttagctaata cgatcggggt caagaaagaa gtgatgatat tcgctccatg gatccctacg
     6121 atccacaatg catggatcaa gaaataaatc accatgacac caagccatgt attcgtcaac
     6181 tgcgtaacaa acccaaatgg gatagcaatc attttgaaga tatccgttcc tagtgcaact
     6241 aataatccat tgatgatcat caccacaaac gcaatgacaa atgttgggat cagagcagtg
     6301 aaagaacgag aaactcctgg cggaacaaca tctggcattt ttatgaccca atttctcttg
     6361 acacacatac aatacaattc gaccgcaata atcgacataa taatagctgt aaagattcct
     6421 gaagttccta aacgttcgac aaaagagccc atcctaatac catttactac agtttctgtc
     6481 tcattgatag aagcaatcaa gctgatttta ccatcagtca tgatcaattc agggatacac
     6541 ataaagaatg caaaaacaga tagtaatgcc cccgtgagcg gattaacatt taatttttgt
     6601 tcatctcgtt caattgacgt taattcataa gcaaacacaa tggcaaagta aatagataaa
     6661 atccccatcg tcattgtatt gacgatcatg tacaagtcac taactttgaa aaaagttgca
     6721 ttaaaaaatc cttctaaaaa tgggaacggt aatggcaaaa tatttaaaac caaaaacatc
     6781 gagcctacaa tcgtgaaagg aatagaagcc atccccgctg ccatgacagc ccgaacgatt
     6841 ttaagttgcg caattttccc cataggtccc atcaggtatt tctgcaaaaa atcaaacatt
     6901 agttattctc ctttcctaaa tataacgctt tttcatattg tttaatacgg atatactgtt
     6961 ggtcttggtt gagtgaaatt ttctttaaac gatactgtat aaatgcactg gcaaaaatac
     7021 cgcttacaac aagaatgaaa acaaataact gtcctccatt gttatttgcc ataaaaggat
     7081 acaagaatgt aaatcctgag gtaaacaaca ctggaatcac taataaatta aagacactct
     7141 ggaacaaaaa ataccacttt gtccatggaa cgttcgtttg gtgttttcga taaagcttta
     7201 cttgttctcc tactgcaggc aaaatagcta aaatcagaat catcggtaaa ctacttccta
     7261 aacttttagc tgatagaagc agaatcatcc attgcatgtt catgaaaaag aaaaaggcag
     7321 tcaggtaacg tacgattaga taacgattga aatacatgtt tttcaaacta agttgctttt
     7381 tttctaattc gattttcctt tttactgtat cctgcacaat gatccttcct ttctatttat
     7441 ttttttgata caaataattc atttcctgtg caacttccaa taatgtcatc gtcgtcatca
     7501 gatgatcttg tgcatggacc atgataattt ccatttctat ttttgtcccg ctggcatatt
     7561 ctttcagaag ttgagtttgt gactgatgcg catgaagaat ctcttcattt gcttgtcgca
     7621 acttctcttc tacttcttca aacatctctt tacgcatcga gcgaaatgct tcatgtatca
     7681 gtgtccgtgc atttccgcta tggaaaatga tatcaaaaga agtcacttgt agtgtttcac
     7741 tgtttatttt ttcagccatc tgttttcctc cttattcgat cagtttgata aatttactgc
     7801 tgaaattctc aaaaataggt tcagctaaga tttcctgctg tacttttagc tggtcaacta
     7861 acctaacgat tgctttagtc acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa
     7921 ctaaaaaaac aaaatgaatt tcttgatatt gatcccactg catcccagaa ggaatcaatg
     7981 caacggctac ctttgtttcg attccaactg gaacagcggg atgtgggact gccactgtat
     8041 cactgaatat gatttgtccc atattttcac gttgctggat ctgctttcgc atttctctta
     8101 tatatccttc ttcttcattt atgctcagct tttgcagcaa atcatgaaga atttcttcct
     8161 ttttcggttc gtacgtataa gtcttgaaaa atttttcaga caactgttcg gtgtaaatct
     8221 gctttttttg ctgtagagaa aatgctggta tcggtctatc tgaggaatga tagggtatag
     8281 attctccaac tacctttcgt attttacgaa catcatcttc attcaaaaaa atactgacat
     8341 gtaaaactgg aattttgaaa aacattgtcg aaagatcaat agaagaaata atcaggtcga
     8401 ttccttttaa agttgtttca ttgatctcat aatagccttt tacacttacc actgtaatat
     8461 ttttaccgaa ttcactgact acgcgattct ttaatagttg ggcactgccg taacccgttg
     8521 cacagataat caatgcatgt agtttatgcg cagctctttc tttttccaat gccgccatga
     8581 gatgcaaagc caaataggcc cattcatctt cattgatggt atagtttttc aaagccggca
     8641 tgttgctgaa atgccttttt actgcgtcaa agactttggg attttctttc tttatctcct
     8701 cagtcaatgg gttctcaatg cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat
     8761 gatcgagtag tccattgaaa agctggagat cctctactaa tgaaaaatga aattcctgag
     8821 acaatctttt cagaactttc gctaattcct ttgatagttc ctgatcattt tgattttctt
     8881 tgtgattcga cttagccatg agatgtaatg tcaaataagc aatttcttct tcgggaaacg
     8941 atacacctgc gattacctca atacgttgaa cgatccgctg cgccacttga tactctactc
     9001 gcttagaaac ttcttgttcg atgcctaatt ccttgatttc aaacccttct cggagccgtt
     9061 tgatccccaa agccaagtgt aaagtcagat tctgtatgat aaaatccgaa acttttaact
     9121 tagcttctct tgtttcatcg agaatgataa tgactagctc ttcaaaactg atgtctggga
     9181 aaaaacggct ttgccctaaa tattctttca atgaatttcc ataatggttt ccaaaaaaag
     9241 tatccatgat aaaatgccgt cgatttcttt cttccccttc gatccatgtc ccttgaccat
     9301 gcttagaaac gatttttagt gaatatggaa tcaatcgctc cttgatttct tgcatgtcct
     9361 ttgtcaaact ggaacgactg atataaagct cttctgccaa atcatccata aaaagaatgg
     9421 catcttccaa aagcaattgg ttcaatatat acttttgtcg atcttcggac tcataaaatc
     9481 cttgcggttc tctagcatat ccaggtacta tttcttgccg actcaaaaac aagtcaaatg
     9541 tcagtctttg taaaatatgc agttggtagc cataacctgg tttagcaatg atcttacctc
     9601 cgtttttttc tactaactct ttgatccgct gcaagtaatt acggattgtc cgatcagata
     9661 ataacaattc agaagctaaa cgttgactgg tgacatactc cttcttgtga tcaatcaaat
     9721 atctcaacag caatttttcc ttctgtttca ttatcatcca ctcctattcc atcatatgtt
     9781 ctcttcgatc acctctgcta acttctggat gcctgtaggg attgggacat acgcttggaa
     9841 agggatacta acaacaggct ttccaacaga ttctcctaat tttttgaatt tatccaaaaa
     9901 catcgtcgtt tgcgggctga tcagaaataa atcaaaatca ctattcttaa tcatattatc
     9961 gccttctgta gctgaaactg catctacgag tacctcttct cttttttgct taaagaaatc
    10021 cgttgtcttt gaggccatca tcgaggatga cattcctgcc gcacaaataa tcaatgcttt
    10081 tttcataaat caatcactcc ttttattttt ataaaccaag catatcttgt aagcgttttc
    10141 tattcaaaat atatatttcc gtagcattgc ggaaaaaatg tgtatcatat cttgaataat
    10201 aatttctggc accgtgatat actagtgata gaattttttc aggaggggat ttagtgagta
    10261 tcacagtgaa tattttgtat acaagtacaa atggacaagc cagaaaattt gcagaagaaa
    10321 tggtcgaaag aaacattgtc aaagcgattc gtgcagaaga aggaaacgag aaatatgact
    10381 attactttcc actagaagat ccagaatctc ttttactgat cgaccgttgg aaagatgaag
    10441 aagcaatcga aaagcatcac aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa
    10501 agtataaatt aaaaatgaaa gtcgaacgtt tcactgattt accgtaaaaa gatacacatg
    10561 gaatgatagc aatttatcat ccatgtgtat cttcactttt tccttatttt ttattcgatc
    10621 tcatctaatg attcaaaccg tgtttttctc ttatttttcg gataagtatt tcagtcaccc
    10681 caatatacat gattcctaaa cataccccaa ggatattctc aaaaatgcgg atcgttactg
    10741 ctcctggaac cccgaataaa ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg
    10801 ttttatatct ataggtagaa caaacaccta aagctagacc acctaatata ccgacccagt
    10861 taaaaggaat aaattggctg ataccaataa ataaaacaca tccaatcaat gagccaatga
    10921 ttcggtctac tgctcgttct ttgaaaccaa tcgacatcaa tccatagcta gatacaatag
    10981 aagaaaatgc aaaccctgcc cacatcaatc gctgaaaagg gacatactct cctgcaacga
    11041 tcaaaagact aatgccaaat gcatagatga gcatccaaat tttttcctgc gaatacatgc
    11101 ctttcaaaaa aatctcttta aacaagcttt tgtctttgtt cttttctcga tgttttctgt
    11161 aaaggagaac tgaaaaccaa caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga
    11221 aattcttgtt caacgaatct ttaggtaacg aataaacaat aaagagatat gaaaagccgt
    11281 acaaagaagc attccccatt ttgggatcac tagcggttgc cattaatatg ctgcttagta
    11341 aaataaaatg aatcaacatg cccataactg accatttgat cagctggaca tacggtgcta
    11401 ctaatagtga gaacatgacg attgctaaac ctatgatcga atgggaaatc ttataaccaa
    11461 agtcaacaaa acgaatactc atcaacatac agaataaaac cacagcgtat ggtttatgct
    11521 tcgattcaaa taatgttgta attataatga taaaacaaat ggcaaacaat acgatgaata
    11581 atgatcgtaa aaacaatgaa caaatataca tgcacttttc ttttttagaa gctgcttctc
    11641 gtattttttg ttttaaaata aacggatcta gttgtagaga ctgataaaaa tccacctgtt
    11701 gtcgtccttt ctttttactt ttatttttta cagtcatcta tgacaggttg taaatactat
    11761 ggaacatcat atggcgttgt cattttcttg tcaactattt ttcctattca tgtttttata
    11821 aaaaaacata tcgttttctt gtatgattaa atcaccacaa aataaacaac tgagagaacg
    11881 atatgcttta tgaatggttc tatcaaaaaa attctgagat caatagacaa aaatttgatg
    11941 attacagagg tctcttacga aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt
    12001 ctcttccctg ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg
    12061 aaattaatgg tggtgaaaaa caagaagaag gaaaccattg tccactttga ataatataat
    12121 catatgccaa tggttatacg cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc
    12181 cattggaacg ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga
    12241 cataaaatca cttctttact gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt
    12301 taggtatctt tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta tttaccaaag
    12361 tcttccaaaa gaattctgtc ttgagtgttg agggttgtga atttcgttct cacgtttccg
    12421 ttgaggataa actgagtttt atttccgcgg atggcgagac agaaaaatta atcgagaaaa
    12481 tttacctaca caaaaaattc ctcgattaac aaactatttc cttagctgca ctaatcctaa
    12541 agaaatagaa ttcttagtta cagacatgaa cgccacctac tttcagctca ccaaacatgt
    12601 tctaccaaat gcgaaattgg tgattgatcg attacttggt ttttccacgc ctttcaagga
    12661 ggcgtaacct ttattcacga gttggttgaa gctttccgag acaaaagacc cgacttgttt
    12721 ttctccttat tgacaaagag ccattaaaac tagagaaaaa attacaactg atcgctgatt
    12781 atttgtagaa agaaaatcaa agtattaccc aataaacttg agtcattagg caaaaaacac
    12841 tgcgaaactc aagaatgatt ttcgcagtgt ttttgtatat tttttattaa ctagtattac
    12901 tacaaaggtt tatttttcac ttctcaacgc agcacaaaat tgtctttact tatttactgg
    12961 ttgggttcaa ccttctctac tccgatacta atatttgttt gtatacccct tctaaaatcg
    13021 gtagtttcca ttcaattgat gggtactagt tgattttaaa aactatgctt attcaatttt
    13081 ttattttagc tcatatattt cgtttctttc taaattttat actgatgaaa acaagtagca
    13141 cacccaatat tgacaatata aactggctaa tgatggtttc tccagttttt ggaagatgct
    13201 tatttccact gcttgttgga ttgtttcctc ttatatctga gctatcagca acttttctat
    13261 tctcttttgt taaaaagatc gccacttctt gttctttttc gatcttgaaa gttactggtg
    13321 tcgcatccaa ttcatacccc gttggcgctt gagtttctac cagctgatat gctcctggtg
    13381 ctaagccatc tatcgccagt tttccatctg ctcctgtggt tagacctgac tgcagaacgg
    13441 ttccattggc atcttgtaac tcaaaaacag ctccttgcag gacttctccg ctttggtcat
    13501 cgatctttgt caacacgaca ccgcctggtg taagtcgatt ttctttcgtc aattctacca
    13561 ccgctgtctg acttctttcg atctcaaact cgattggtgt cgcatccaat tcataccccg
    13621 ttggcgcttg ggtttctacc agctgataca ctcctggtgc taagccgtca atcgccagtt
    13681 ttccatctgc tcctgtggtt agacctgact gcagaacggt tccattggca tcttgtaact
    13741 caaaaacagc tccttgcagg acttccccgc tttggtcatc gatctttgtc aacacgacac
    13801 cgcctggtgt aagtcggttt gctttcgtca attctaccac cgctgtctga cttctttcga
    13861 tctcaaactc gatcggtgtc gcatctaatt cataccccgt tggcgcttga gtttctacca
    13921 gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct c
//
LOCUS       DAZKCZ010000066        13923 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000066 DAZKCZ010000000
VERSION     DAZKCZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.066"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..471
                     /locus_tag="ACG8KN_001780"
     CDS             157..471
                     /locus_tag="ACG8KN_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9188835.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            492..869
                     /locus_tag="ACG8KN_001781"
     CDS             492..869
                     /locus_tag="ACG8KN_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9188836.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            879..1652
                     /locus_tag="ACG8KN_001782"
     CDS             879..1652
                     /locus_tag="ACG8KN_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188837.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1674..3077
                     /locus_tag="ACG8KN_001783"
     CDS             1674..3077
                     /locus_tag="ACG8KN_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9188838.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3259..4443
                     /gene="mobT"
                     /locus_tag="ACG8KN_001784"
     CDS             3259..4443
                     /gene="mobT"
                     /locus_tag="ACG8KN_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9188839.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4440..4730
                     /locus_tag="ACG8KN_001785"
     CDS             4440..4730
                     /locus_tag="ACG8KN_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188840.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4727..4948
                     /locus_tag="ACG8KN_001786"
     CDS             4727..4948
                     /locus_tag="ACG8KN_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188841.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            4990..5769
                     /locus_tag="ACG8KN_001787"
     CDS             4990..5769
                     /locus_tag="ACG8KN_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFP9188842.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            5830..6330
                     /locus_tag="ACG8KN_001788"
     CDS             5830..6330
                     /locus_tag="ACG8KN_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9188843.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6386..7024
                     /locus_tag="ACG8KN_001789"
     CDS             6386..7024
                     /locus_tag="ACG8KN_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFP9188844.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7096..7491
                     /locus_tag="ACG8KN_001790"
     CDS             7096..7491
                     /locus_tag="ACG8KN_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9188845.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7475..9928
                     /locus_tag="ACG8KN_001791"
     CDS             7475..9928
                     /locus_tag="ACG8KN_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9188846.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            9925..11937
                     /locus_tag="ACG8KN_001792"
     CDS             9925..11937
                     /locus_tag="ACG8KN_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9188847.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETKY
                     SKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSNATGYL
                     VKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYVAYTGL
                     VKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTKIVLPN
                     TDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDTNNGED
                     RENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFSQVLFI
                     IFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTMLYSLSA
                     SSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNRQTRRF
                     QRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQQGGIG
                     QRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDFKQSMA
                     DTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRKRQQTV
                     TPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            11934..12956
                     /locus_tag="ACG8KN_001793"
     CDS             11934..12956
                     /locus_tag="ACG8KN_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFP9188848.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            12973..13902
                     /locus_tag="ACG8KN_001794"
     CDS             12973..13902
                     /locus_tag="ACG8KN_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9188849.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         4714 a         2367 c         2781 g         4061 t
ORIGIN      
        1 gctcagtacg gagcgtctat ttttttaccc aattttgaaa ggaagtgaac ttatgaaaat
       61 aaaaaatcaa gaatcaaaag gtcgctctcc cccctttaaa accatcacac aaataagatt
      121 cattcaaaca taaaaacaga aaggataggt aaaaatatgg aacttaaata tgtcattccc
      181 aatatggaaa aaacatttgg acaattagaa tatgctggtg aagggaacat tgaacaacgt
      241 cgaataaatg gcagaccaac cattctctct cgtagttaca atttatactc aacgattcaa
      301 cgagcagatg atattgttgt cattcttccc tccgaagctg gcgagaaaca ttttgaatcc
      361 gacgaaaaag tcaaactcat caacccacat attaccgcag aaggttataa aattggcaat
      421 cgaggattta ccaattacat tatgcacgca gacgatatgg tgaaagcata agaaaggaaa
      481 ggtgaaaaaa tatgagatta gcagaaggta ttgtgattga aaaagaccag acatttggca
      541 cattgaaatt ttccgccttg cgtcgtgagg ttcgtatcca gaatgaagat ggtacggttt
      601 ccgaagaaat taaagaacgt acctatgact taaaatcaaa aggacaagga cgcatgatac
      661 aagtcagtat ccccgcttct gttcctttga aagaatttga ctacaacgca gaagtggaat
      721 taatcaatcc tgtggcagat accgtagcga cggctacttt tcgaggggca gacgttgatt
      781 ggtacgtgaa agcagacgat cttgtattaa aaggacagac aaaacctcat cagaaatata
      841 aaaaagaagc gtccgaaagt gaaaaatagg tgttgcttat gacaaacaca ccattgattt
      901 tgaaagaaat tccgaaggat gaagccattt cttttattcg gcaataccat tattcaaaga
      961 tactccctag attgtgtaaa tactttctag ggattttttc agaagaaaaa ttattaggcg
     1021 ttgtggaatt aggctggggt acgcaacccc tacaaaccat acgcaaactc tttcccgaca
     1081 gttcccttca aaccacagat tatttagaaa ttggaaagat gtgttttctt ccagaaatga
     1141 atcaaaccaa ttattttggc agtcaagctc tttcagctct aattaagtgg ttgaaagaac
     1201 atacggactg tcatttttta tatacactgg cagatggaat tgagggaaaa tgtggctatg
     1261 tctatcaagc gtccaacttc ttttactgcg ggtatttcaa gacaagtgtc tatcgtgata
     1321 agcagtcatg ggaaaagata caccctcgaa gtgctcgcct tttattagag gaaaatgctc
     1381 gctttgaaca ggtagagaaa aagcattggc tttcccaggc attttgcgaa tacaaaggga
     1441 ttgagaaaat caatggacgc atgtttcgct atctctatcc gttgacaaaa gaagcgaaaa
     1501 aactgttagg acacactctc tatcgacgtc attattatcc gaaagaaaaa aatttacgct
     1561 ttgaaaagcg aatcgcttat cagaaatatg aagccatttc tcaaccaact tttgataaac
     1621 aggctcgtat ctacaataca caattatttt gataggtgga aaggaggatt tttatgccat
     1681 tgcttcatga cagaggagag cgaatcaaag ccaacgataa gcacttggtc tatcattttg
     1741 tactaatttc attgctggta gtttttctag tcgtcctact cccttttcat tggagagcgt
     1801 tacaatcggt gaactggcaa gcagttcagt tagatacatt tttctatcaa cttcatacgc
     1861 catacttttg gagcagtatt ctcactgctc tttttgtatg cggacttatc agctggcttt
     1921 attatcacta tcgtatagat tctataaaac agttagagca ccgtcaaaag ttagctcgta
     1981 tgattttaga aaatcattgg tatgaagccg aagaaactca atcagaggga ttctttaaag
     2041 atttacccag caagaaaacc aaacaacgaa tccgccattt tcctaaaata tattatcgaa
     2101 tgaaagatgg actaatccat attcatgtag aaatcacctt agggaaatat caagatcagc
     2161 ttttgcacct agaaaacaaa ctagaaacag gattgtattg tgaattagtg gaaaaaacgt
     2221 tgaaagattc ttatgtagag tatgtcctac tttatgatac tattggcaat cgaattggca
     2281 ttgaagacgt ggtagctcaa gatggacgtg tcaaattgat ggaatcggtc tattgggagt
     2341 tcgattctct ccctcacatg ttaattgctg ggggcactgg tagtggaaaa tcctatttta
     2401 ttctcacatt gattgaatct ctcctgcata caaatgcaaa gctttatatt cttgacccta
     2461 aaaatgctga tttggcagac ttaggggcgg tcatgaataa tgtctattac cgtaaagaag
     2521 atatgttggc atgtatcaat caattttacg aagatatgat tgatcgcagt gaaaccatga
     2581 aacaacaccc taactataaa acaggagaaa attatgctta tcttggtttg tctgccaatt
     2641 ttcttatttt tgatgaatat gtggcaatga tggatatgct aggtcgtgaa agtaccaccg
     2701 ttattcataa actcaaacaa attgtaatgt taggacgaca agcaggcttc tttttaattc
     2761 ttgcctgtca acgtccagac gcaaaatatt ttagtgatgg gattcgggac aactttaact
     2821 tccgtgtggc tttgggtcgt atgtctgaaa tgggctttgg tatgatgttc ggaagcgata
     2881 cacaaaaaca atttttctta aaacccatca agggaagagg ttacgtggat acaggaaaaa
     2941 gtgtgattag tgagttttat acaccccttg taccaaaaag gtacgatttc ttaggagaaa
     3001 taggaaaagt cattcaaaaa aagcaatctg agcctgtacc gcacgaagtg aaaggtacag
     3061 gttcagactt gctttgattg gtgtggcgtt agccacgcca atcctttagc cccctctatc
     3121 taacaggggg gcacaaatac aactgaaaac aggcaattct catcaaccaa tgttgatggt
     3181 acaaaggatt gcggaaaata tgaaaggtgt aaactcaagg gtcatagttt acatcttttt
     3241 tagattggag gtttgcgaat ggaaaacgaa aaaatttgga ttaaggaatt gaaagaaaag
     3301 cgactggctt atggcgtttc tcaaaataaa ctggcggttg cttctcatat taccagacct
     3361 tacctatcgg atattgaaac aggcaaggca gtccctactc aacaggtgaa agaagattta
     3421 ctgaacgctt tggaacgctt caatcccgat aacccattgg agatgttatt cgactatgta
     3481 cggattcgat tccctaccaa tgatgtggct caaattatcg gtgaggtttt acgtttgaat
     3541 atggattata tgctacacga agactttggc tattattctt atcccgaaca ctatcgtttc
     3601 ggtgatattg ttgtcctagt ctctcatgat gtaagtaaag gtgttttatt agaactgaaa
     3661 ggaaaaggct gtcggcaatt tgaaaacttt ctattggctc aacatcggag ctggtatgat
     3721 ttctttcaag attgcatgga acataaaggt attttcaaac gcttggattt agctatcaac
     3781 gataaaacag ggattttgaa tattccagat ttggcaaaga aatgtaaaca ggaagaatgc
     3841 atttccgtct ttcgaagctt taaaaattat cgcagtggtg aattggttca ccgtgatgaa
     3901 aaaccataca tgggaaacac actttatatt ggctcattaa aaagtgaagt ctatttctgt
     3961 atatatgaga aagattatga gcaatacgtg aaaaatgatg ttcccttaga agacgcagaa
     4021 gtaaagaatc ggtttgaaat tcggttaaaa aatgaccgag ccacccaagc catgaaagat
     4081 ttattggcac atcaacaggc ggaaaaaaca gcctttgaaa ttatcaatcg ctatatccgc
     4141 tttgcagata aagacgacac taaacgtcgg agtgattgga aaactaatga acgttgggaa
     4201 tggtttatcg gtaaaaatcg tggagcatta cgcctaacca cacagccaga gccttattct
     4261 tttgaacgaa cattgaactg gttacatcat caagtcgccc ctacattaaa aatcgcaagc
     4321 attttagatg ttctcaacgg tacaaccatt atctccacca tgattcagga agccaaactg
     4381 acagaaaaac atgagaaatt aattgaacaa caacatctag ccatggagga tttgattaca
     4441 tgagcctttt aacaaaagaa ttaaagaagt tagggtttca gtgtggcatt gagtttcaag
     4501 cgtatataca aaatacaggg aaatatacgt ctctcattat tgagggaaaa cggcaagctg
     4561 gtgataccat ctatacctat gatttttaca aggtgagatt ttataataat tataccaatc
     4621 gagtaaccgt ctatggagag catttaaccc cttttcaatt attaaggcgt gtaaaaagct
     4681 atatctatta tcgagaaaaa tatcttaagg aaaggaggac aatcacatga attttgggca
     4741 gaatttgtac aactggttct tggataatgc acagtcattg gtattgttag cgattgttgt
     4801 cattggactg tttttaggtt ttaaacgaga attttcaaaa ctcattggct tcttgattat
     4861 tgctctaatc gccgtcggac ttgtgttcaa tgcagctggc gtgaaagatg tgttattaaa
     4921 cttatttaac cgaatcattg gtgcttaaca gatatttttc atgataaaat aggaagtata
     4981 tattttgtta tgaaaggatt tatgaatatg tttacaaatg atcaatttaa atctctttta
     5041 attaaaacat tagaaagaaa gagctcttca aattattatg aaggaggtaa cgagattgtt
     5101 tctaaaatga aaatatcaga ggtaacttta gatgagactg acgattttac ttattataaa
     5161 ggatataaaa agggctggaa tacgctttgc acatatctta aaataagagt tccttttgat
     5221 gatttagact tttttgaaag tcataaagat ttgattaccc agacagcaag ctccatatat
     5281 gataaacaag gtgataatgt attagtggat actattttag taccacttcc agagaattac
     5341 gaagtaatta actttagtca gttgaaaatt agtgatgtgg tttcacaagc tattgaagac
     5401 gctgagtctt ttatgagtaa tggtgaatat caacgtgcct ttgacagagt tcacactgct
     5461 tttcatggat atttaattga aatactaaaa aaatatgaaa ttacagttcc tagagacgaa
     5521 aatctatcta aattatatag taggatacaa caattaattg aaaaagaaat tcaacctact
     5581 gaacttgctg atatagtgaa gacaacgata aggtcatcaa atggaatgat tagctctctt
     5641 aatgaagcaa gaaataggca ttcacttgca caccctaata caaatattat tggaaagaga
     5701 gaagcaaaac taataatagg tatttcaagt acagttacag attatattag cggatacctt
     5761 gataagtagc gtcattaact gattgctact ttttttatga ctatttttat aaaaaggatg
     5821 tgataagaat tggaaatgaa agtctatgtg gcaaatttag gacggtataa tgaaggtgaa
     5881 ttagtcggag catggtttac tccaccaatt gatgaggaag aaatggcaga aagaatcggt
     5941 ttaaatgagg actacgaaga atatgcaata catgattttg agcttccttt tgatgtggac
     6001 gaatacacgc cgattagtga aatcaaccgt ctatgtgaag ccattcaaga aatcgaaggt
     6061 acacctattt ataatgaact caaagaaatt caaggcatgt ggtttagtag cttggaagaa
     6121 ttactggaaa acaaagagga tattcattgt tatagtgatt gtgattcgat ggaagatgtc
     6181 gcccgttatt acgtggaaga aacaggacag cttggtgaag tcccttccaa tttacaaaat
     6241 tatattgact accaatcttt gggacgtgat atggaaatag agggtaatta cctagttacc
     6301 tctcatgggg tatttgaata ttgtcagtaa gtttaacaat ccgtgttata attaaattaa
     6361 taaatagcac ggagaggtat aaattatggt aaatgtattt gaaaatctta aactattaaa
     6421 actagacatg aaagataaag gctgggtgat agattccttt tactttcgtt acaaacagca
     6481 aaattatatt gtacttgtta agctatttga aaaagaggaa aaagttccag aatatgctct
     6541 gttaaaacta gaatttttaa aggaaaacga tttttcagat atgctagctg tctatgctaa
     6601 ttcggtaaaa ttatatacag acacgaaaac aataagagaa tattttggta tcgaatacag
     6661 tagtaacttg ggagatgttt tatttcaatt tagccaaact cttgctcgat ttattccgac
     6721 agaagttagt gagaagaaaa atgaagacca aaaagaagcc atgtgtttct cattaagtca
     6781 aagtgattca gaagatccca gaaaaaaata ttgtttttca gttagaagaa atcctttaaa
     6841 gggaaatggt gagctgggaa aacgaagtcc ttttaatgac aataaaacga gattgtatag
     6901 acccagctta tatgaacgat tagggtcaga taccaattta agtttcagat attcaatgaa
     6961 tccagatgat gaagaaactg atgaaggtat tattgcaaaa tggacaaaaa ataaaattga
     7021 atagttaaag cattgaaaga gcaatcacct attttgattg ttctttttct atttttacgg
     7081 aaaggacgtg aagacatgaa aaaaataaga agttacacca gtatttggtc agtagaaaaa
     7141 gtgatttatg cgattaatga tttacaactc cctttcccaa ttacctttac gcaaatgaca
     7201 tggtttatcg tttctttgtt tgtcgtcata ctctttggca gtttaccacc actttctctg
     7261 attgacggag cactactcaa atacttaggg attcccattg ttctcactgg gttcatgtgc
     7321 caaaagacct ttgacggaaa gaaaccctat ggatttctaa agtctatcat tacctatgct
     7381 ctacgcccta aagtaaccta cgctggcaaa caaatacgat taaaaaaaga aacaatgaat
     7441 gaatcgatta cgattgtaag gagtgaacaa ctagatggca tatccgatta aatatatgga
     7501 agataatctc gtatttaatc atgatggcga agtattcgct tattatgaat taattcccta
     7561 taattacagt tttttaagtt ccgatgaaaa aattcaagtt catgataatt tctgtcaatt
     7621 gattgcccaa aatcgtgatg gcaagattca tgctctgcaa ctagcaaccg aatcaagtat
     7681 tcgagccaca caggaacaat cgaaaaaaga aatcactgga cgattaaaag agatagctta
     7741 cgaaaaaata gatgaacaga ccgaagcgtt aatttctatg attggtgaca atcaagtaga
     7801 ctatcgcttt tttattggtt ttaaactcta cctcaatgaa caggaagtat ccgtaaaaaa
     7861 tgttgggaaa gaaattcaaa aagcatttgc ggaattcttt catggtgtga atcacaaatt
     7921 aatgggcgat tttgtttcta tgcctatggc ggaaatcgaa cgcttttcta agatggaaca
     7981 gttgttagaa agtaaattat ccagacgctt tcagattcgt aaattggaga aagatgattt
     8041 tggttatatt attgaacatc tttacgggca aacaggtgtc ccttttgatg actacgacta
     8101 ccatttaccg aaacgatacg aagataaaaa agtgttggta aagaaatacg atattctcaa
     8161 acccactcgc tgtttaatcg aagaaaatca acgctattta cggattgaac aggaagatac
     8221 cactacttat gtggcgtatt ttacaattaa tagtattgtt ggtgaattgg atttccctaa
     8281 tagtgaaatt ttttattacc agcaacagca gtttactttc cccgttgata ccagtatgaa
     8341 tgttgaaatt gtcgccaata aaaaagcctt atctaccgtt cgcaataaaa agaaagaact
     8401 gaaagattta gatagccacg catgggaaag cgacaacgaa accagtacta atgtggtgga
     8461 cgctttagaa tctgtcgatg aattagaaag taatttagac caaaccaaag aatctatgta
     8521 caaactaagc tatgtggtac gggtatcagc aaagaatttg gacgagctga aacaacgttg
     8581 tgatgaagta aaagattttt atgatgatct gtctatcaaa ttagtacgac cttttggcga
     8641 tatgctaggc ttgcataatg aatttattcc ctcaagcaag cgatatatca atgactatat
     8701 tcaatatgtc acgtcggatt ttttggctgg tcttggtttc ggtgctacac aaatgattgg
     8761 tgaaaattct ggtatttacg tcggttacaa tctcgataca ggcagaaata tttatctcca
     8821 acctagcctt gcaagtcaag gaattaaagg ctctgtaaca aatgcattag cgtccgcttt
     8881 tattggttca ttaggtggcg gaaaatcatt ctctaacaat atgctggtct attattcagt
     8941 actctttggc ggacaggcaa ccattgttga cccaaaagct gaacgtggtg aatggaaaga
     9001 aaccttacct gatattgccc atgaaatcaa tattgttaat ctcacgagtg agaacgacaa
     9061 taaagggtta cttgaccctt atgtgatttt aaaactcccg aaagattcag aaagccttgc
     9121 cattgatatt ctcacgttct taacaggtat ttccagccga gatagcgaga agtttcctgt
     9181 cctgcgtaaa gctattcgga gtgtaactca aagcaaacaa cgagggttgt tattggtgat
     9241 tgatgaattg cgaaaagaag atacgcccat cagcaatagt attgctgacc atatcgaaag
     9301 ctttgtcgat tatgattttg cccatctgtt attcagtgat ggcagtgtca cccaatccat
     9361 tagcttggaa aaacaactca atattattca agtggcagat ttggtcttac ccgattccaa
     9421 tagctctttt gaagaataca ctaccatgga attattaagt gtggcaatgc tgattgtcat
     9481 tagtaccttt gctttagact ttatccactc tgaccgttcc atttttaaaa ttgttgacct
     9541 tgatgaggct tggagttttt tacaagtggc acaaggaaaa gctctatcta tgcgattggt
     9601 tcgagcagga cgtgccatga atgcaggggt ctatttcgtt actcaaaacg cggacgattt
     9661 actagatgaa aagatgaaga ataatattgg cttgaaattt gcctttcgtt ccactgacct
     9721 agtggaaatc aaaaagacct tagaattttt tggtgtggac aaggaagatg aaaacaatca
     9781 gaaacgctta cgtgaattag aaaatggaca atgtttagtt tcagacatct atggtcgtgt
     9841 aggggttatg cagtttcacc cgatttttga agagttgctt catgcctttg ataccagacc
     9901 gcctgtgcga aatgaggtgg aagaatgaca caaaaacaaa agaaatttct tcactatagt
     9961 tggataacgc tcctttgtgt gggcgttatt ttacttttat taggcacgct gggcaatgca
    10021 gtacaagcga caggcttagt tgatgaaacc aaatactcca agtatggttt gaatcaatat
    10081 cagcttgatt attatgtgga caatagctgg ggctggcttc catggaattg gagtgatggc
    10141 ataggacaat cggtgatgta tggattgtat gccattacta attttatttg gacaatttct
    10201 ctttatctat cgaacgccac aggttatttg gtgaaagaag cctatgcctt agatttcatt
    10261 agtcaaacat cccaagcaat tggcgaaaac atgcagacga ttgcaggcat tacaccgaat
    10321 ggcttttcca gttcagggtt ttatgtcggt ttcctccttc tttttattct cattgttgga
    10381 atctatgttg cctatacagg attggttaag cgagaaacga cgaaagtgat acgagctgtt
    10441 ttaaactttg tcgtggtgtt tattctatcg gcttccttta ttgcgtatgc acccgattat
    10501 attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg ccagtctgaa tattgggaca
    10561 aaaattgttc ttcctaacac ggatagtcaa gggaaagaca gtgtggacat gatacgtgac
    10621 agcttattct ctgttcaagt aaagcaacca tggctattgc ttcaatatgg ctccactgat
    10681 gtagaagcgt taggggaaga acgtgtggaa agtttattgt ctaccagtcc agatacaaac
    10741 aatggtgaag atagagaaaa tgtagtgatt gaagaaattg aagatcatga caatacctat
    10801 ttaaccctac cgaagacgat ttccagatta ggaacagtct tctttttgtt tgtattcaat
    10861 attggtatct cgatttttgt tttccttcta acaggaatca tgattttttc gcaagtgcta
    10921 tttattattt tcgctatgtt tttgccaatc agtttcctat taagtatgtt gccgagtttt
    10981 gatggcatgg gcaggcatgc cattaccaag ttatttaatg tgattatgac aagggctggt
    11041 attaccttga ttattaccgt tgcctttagt atttccacta tgctttattc gctgtcagca
    11101 agttctccct ttttcatgat tatgttttta caaattgtga cctttgcagg gatttatttt
    11161 aaactgggcg atttaatgag cctattcact ttacaaagca acgactctca aaacatgggc
    11221 agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac aaaccagaag atttcaacgc
    11281 agtatgagta agatttttcg tggaaaagca gggtcaacga agcaaaagac aatgacaaca
    11341 aagcagaatc aaaccaataa agctaaccat actcgtcaaa atgaacggac tacaccaaag
    11401 aaagaaacag cactttccaa tgtgaaaaag caacaaggcg gtatcgggca acgattaggt
    11461 gaaaagacag cccatgtatt ggacacgaaa aatcgtctag tcaataaaag aaagcaggca
    11521 aaacaacaga tacaggcgac accaacccat gtcaaatatg cccttcatca aggaaaagag
    11581 aatgtcactc ataatgtaac agactttaaa caatctatgg cagataccaa agctcaacgc
    11641 aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga acattgctca aaagcgtttg
    11701 gagctggata aagcaaaaga aaaaagacga aaagaaaacc catcatctcc tgttcagaat
    11761 aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc ctgttttgtc acaaaacact
    11821 aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa aacaaaagac accaccgaag
    11881 caattcaatc agaggaagct aaataaaaga acggataagc gaggacaaca aacatgaagc
    11941 taaaaataac agtgatttgt agtgtggtct tctctctctt cttttttctc ttattattcc
    12001 tagtcatttt ctttgcagat gatacggaca gtggcgaaaa caacaaggat tcttctattc
    12061 cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca ccgtccgttg attgaaaaat
    12121 atggtaagga atatgggatt gaagactatg tttcttatat cttagccatt atgcaagtgg
    12181 aatcgggcgg tacagcagaa gatgttatgc aaagttccga atctttgggt ttaccgccca
    12241 acagtttgag taccgaagag tccatcaaac aaggggttaa atatttcagt gaattattaa
    12301 ccagtgcaga acaacaagga gtggatatag attctgttat ccaatcttat aattatggcg
    12361 gtggcttttt aaattatgtg aggagtcatg ggaaaaaata tacctatgaa ttagccgaac
    12421 agttttctaa agagaagtcg ggtggtcaaa aagcagacta tcccaatcct atagccatac
    12481 ctgtgaatgg tggttggcga tataactatg gaaatcagtt ttatgttcag ttggtttcac
    12541 aatacctaac cgacacttcc ccaacagaat ttgatgatga aaccgtgcaa gtcattatgg
    12601 acgaagcctt gaaatacgaa gggtttccct atgtctttgg aggggcgtcg cctaccacct
    12661 cttttgattg tagtggctta atacaatggg tgtatgacaa agctggcatt tctctccctc
    12721 gtgtcgccca agatcagtat gacgctacgc aagaaatctc tatggaagaa gcccaagcag
    12781 gcgaccttat cttctttcat tcaacctata atgcaggcac atatgtgact catgtggcga
    12841 tatatttaga gggaaatcgc ttttatcatg caggcgaccc aatcggttat ggcgacttaa
    12901 gcagtcgtta ttggcaagac catctgattg gggctagacg tgttattcat aactaagaaa
    12961 ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa agaaggagaa accaaagaaa
    13021 tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta aagttggcac gcataagaaa
    13081 ttaacctttg ttttatgggt tttattgatt ggtagtgtcg gatttggaat ctataaaaat
    13141 tttactgcca ttgacactca tactgtccgt gaaacagaaa tcattaaaca gcagattgtc
    13201 gataccaacc aagtagaaag ttttgtgaaa tcctttgcga aagactactt ttcgtggcaa
    13261 caatcccaag aagccattga taaacgaaat gaaaaattga cccattattt gacggaagaa
    13321 ttgcaggtat taaatgaaga aatgattcgt aaggatattc ctactagttc tagtgtgaac
    13381 gatatacagg tgtggcaggt ttctcaagtg aatgagaaca cctttgaggt actgttttct
    13441 gttgaacaag ttattaccga agacaaagac aaggaaacta tatcgtctag ctttcatgtg
    13501 gttgtccata tagatgaatc agataatatg gttattatca aaaatccgac aatgagtaag
    13561 aaacctcaaa aatcggacta ccaacccaaa cagctagaaa gtgaccatac agtagataca
    13621 gaaacgatgg acgaaatcac tagcttttta gaaacgttct tccagctcta tcctaccgcc
    13681 acagaaaaag aattgactta ctatgtaagt aatcatgtgt tacctatgat taacaaggaa
    13741 tatgttttcg aggaattggt gaatcctatc tttaccagaa aagataacca agtaatcgtg
    13801 aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa cccaaatctc gcaatttgag
    13861 ttgatattag aaaaacaaga taattggaag attgtaaaat aacaaatatt ggtacatgat
    13921 tac
//
LOCUS       DAZKCZ010000067        13659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000067 DAZKCZ010000000
VERSION     DAZKCZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.068"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..1115
                     /locus_tag="ACG8KN_001795"
     CDS             177..1115
                     /locus_tag="ACG8KN_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9188850.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            1127..2038
                     /locus_tag="ACG8KN_001796"
     CDS             1127..2038
                     /locus_tag="ACG8KN_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9188851.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            2062..3534
                     /locus_tag="ACG8KN_001797"
     CDS             2062..3534
                     /locus_tag="ACG8KN_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9188852.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            3585..4196
                     /locus_tag="ACG8KN_001798"
     CDS             3585..4196
                     /locus_tag="ACG8KN_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9188853.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            4226..6139
                     /locus_tag="ACG8KN_001799"
     CDS             4226..6139
                     /locus_tag="ACG8KN_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFP9188854.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            6267..9248
                     /locus_tag="ACG8KN_001800"
     CDS             6267..9248
                     /locus_tag="ACG8KN_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFP9188855.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            9245..10906
                     /locus_tag="ACG8KN_001801"
     CDS             9245..10906
                     /locus_tag="ACG8KN_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFP9188856.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            10946..11878
                     /locus_tag="ACG8KN_001802"
     CDS             10946..11878
                     /locus_tag="ACG8KN_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFP9188857.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(12237..13499)
                     /gene="guaA"
                     /locus_tag="ACG8KN_001803"
                     /pseudo
     CDS             complement(12237..13499)
                     /gene="guaA"
                     /locus_tag="ACG8KN_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
BASE COUNT         4384 a         2251 c         2838 g         4186 t
ORIGIN      
        1 ttacaagtga tgtaaacgct ttgcaaacag ggctacttat gattgtgaat acagtttccg
       61 tttaaaaact attctattgt agaggtttaa taaactaaaa ataatgtata aaaaattaat
      121 gtaagcgatt aaccaagtgg gtataatcaa ggaaacgctg agagaggaga gacaaaatga
      181 agctaaagaa agggttatta ggaaatctaa aaaaaaaccg agcattgttt attatgcttt
      241 taccaggtgc gttatggtta tttatatttt gctatatacc aatgtttgga gttgtaatcg
      301 cttttaaaga tttcagaatt tcatcaaggg gattctttac aagtttgttc aacagtcctt
      361 gggttggctg ggaaaatttc aaatatctct ttgcttcgca agatgcttgg atcattactc
      421 gcaatacggt tgtttataac gttgtaggta ttttgctaag tactttttgt acagtgtcat
      481 tagcaatcat tctgagccaa atggcgaaca aacggttatt gaaaaagatg cagacattta
      541 tgatttttcc taatttttta tcatgggtag tcatcagtta ctttgtttac gcatttttga
      601 gtacggataa aggattaatt aatattttac taagcaattt tggtgtggaa cccataaatt
      661 ggtatagtga accgaagtac tggcctttta ttattgtttt tacgagttta tggaaaggcg
      721 taggctatgg atccatcatt tattacgcgt caattatggg gatcgatact agttattatg
      781 aagctgcttt agttgatggt gccagtaaat ggcaacagat aaggaatgta gtcttgccac
      841 atttaagatc gattattgtc attatgttga ttttgtctgt tggtagtatt tttcgagctg
      901 attttggatt gttttatcaa ttgccaagaa attcgggggc tctttatgat gtgacgaatg
      961 tattagatac ttatgtctac cgtggattaa tgaatagcgg agatatcgga atgtctacgg
     1021 cagcgggatt atatcagtca tcagttggct ttgtcttgat tttgattact aataagatag
     1081 tcaacaagtt agatcctagt tcagcactat tttaggagga atcaaaatga tatcaaaatt
     1141 aaaacggcag atgtactttt cgctgccagt cgaacggttt ttcacaatat tactaatctt
     1201 atttactctt agctgtattt ttccctttat tttcgtaatc gtcatttcat taacagatga
     1261 aaaagcatta caactcaacg gttacgaatt attgccggct caatggagca ctgatgcata
     1321 tcaatactta atacaagatg gcggacagtt acttcggtca ttaggcgtta caattatgat
     1381 tacagtgatt ggaacgttga ttactgtttt tatgacggga acctatgctt atgttttatc
     1441 aagagcctct ttcccttacc ggaagttctt tactttttat ctttttttta ccatgttatt
     1501 tgcgggaggg atggttcctt cttacttagt gatgacgaag atgcttggct taaaaaatac
     1561 aatttgggct ttgattctac ctcttgcttt tagcccatac aatgtcatta ttttaaggac
     1621 attctttaag aagtcgattc cagaatcgat tatcgaatcc gctaaaatgg atggctgtag
     1681 tgagtttcga gtttttttcc aaatcgtttt gcctttagcg atcccagggg ttgcaacaat
     1741 aggtttattt tcaagcttag gttattggaa tgactggttc aacgcattgc tgtatattga
     1801 caccaacaaa ttgattccat tgcaatattt gttaatgaag attcaaaatt caatggaatt
     1861 cttagcaaat aataatgaca ttacattagc gcagcaacaa gcaattcaaa atagccttcc
     1921 tcaagaatca acacgaatgg caatggtcgt agtagctact ttaccgattg caattgttta
     1981 tccgttcttc caaaaatact ttgttcaagg attgacgatc ggtggagtga aagaataaaa
     2041 aagaatagga gatgttaaaa gatgaaaaag aacatgcaga aatatttgat tggtggtgct
     2101 tctgtactaa cccttggcct attaggtgct tgtggtagca gtaatggcga agcaaataat
     2161 gaaacaggaa ccacaaaagt tacttggtat caggttggag aagaacccaa agattttgat
     2221 gaggtcatga caaaagtcaa cgaaaaatta aaggaagaaa tcaatgtaga gttggatatg
     2281 cggctgattc cggacgggga ttatcagcaa aagcttggtg tgatgatcaa ttcaggagaa
     2341 gaatatgata tttgtttcgt caatggcaca gattatgtga attatgggaa caaaggtgct
     2401 ttcattagtc tcaacgatca tttggataaa gagttgaaag attatgcagc tgaattgaat
     2461 gaaggattta ttgaaggtgg ggcaattaat ggcgaaactt atggattgcc agtcaataac
     2521 gatattgcca caacggaata ttggaccttt aataaagatt tggtagatca ataccaattg
     2581 gatgtagaga atgttgaagg tgtagaaaca ttggctccag tattagaaaa gtttgcagaa
     2641 aaaaatactg atacgaatat tacgccaatc ggtatgaccc gtaccagtgt ctgtattcct
     2701 tatgacaata ttatcggtgg tggtgtaccg ctagcggttg catttgaagg cgatacacaa
     2761 acagttgtta atacttatga gactccagaa atgatgactt atttgaaggc tatgcatgag
     2821 ttttataaaa aaggatatat tttgaaagat gcagccactg ttacgacaga tccatttcga
     2881 attgatagta aaaactggtt tttaggtcgt aactcaagtg gttatcaacc attagcacaa
     2941 aaaaaattag aagctctagc gggacatgga ttaattttaa aaccagctgg aaaagctttt
     3001 aaaacgactg gatttgcaag agtggcaatg catgcggtat ctgcttcaag taaacatccg
     3061 gaagcagcct tgaaattgtt gaattatgtg aataccaatc aagaagtagc ggatttaatg
     3121 gtttatggaa ttgaaggaaa acactatgat cgcaatgaag atggttcgat tactttaaca
     3181 aactctgatg attattatca aggcgcttgg gcattggtaa atacttatct agtttcaaaa
     3241 acttcaccga ttacagaaga aagtaaaaaa ctagaagaag aaagtaaaaa gtttcaaaat
     3301 gaagcaccga tgtctcctat tttaggtttc tcttttgacg cttctgattt tcgaacagaa
     3361 attgcaagtg tacaaaatat cgtgtcacaa tatgaagtac ttttaagctc gggaacagtt
     3421 gaccctgaac aatatgtacc agagttgtta gaaaaactag aaaaagctgg attggataaa
     3481 attcaggcat ctgctcaaga acaatacgat gaatggcgaa aatctaacaa ataatttttt
     3541 actaaatgaa aagaatagac taagcagtta gtggggggat tcaaatgaac gaaaacaaat
     3601 ggaccaaagg atttaattgg gtttttcaat tgttgttact aaactttact tattttatac
     3661 taatttgttt aggatttgga attttaggtt ttttccctgc tacgatagct ttgattgctg
     3721 gcgttgtgaa tcgaaaaaaa tatggcttgc aagagtctgt gaagttcatg agtaaaatct
     3781 ataagaaaaa ttttgttaga tataatctat attgctggtc agcaactttg ttgttggccg
     3841 gcaatattac tacgatctgg ctttgttggc agcaaagcgc accattgttt tatttattaa
     3901 gtacattatt atcggtaatt acactgttcc tagtctttgc aatgttgaat tgggtcgtga
     3961 tcaaaagtct tgggttgtgg atgatgggag accgactgat tgactatttg cttttcacag
     4021 caacgatgtt gccaacggta gtcatgcaag gcttggtgct tggaatttat ttccgaatat
     4081 tgatgatttt gcctgtgatt tttctgtttg gcggaacagc tgtaacggta atattgatta
     4141 gtaaattaac tgaaagagcg ttagcaaagt tgccttgtca gatacaatcg cactaatttt
     4201 ttcacaaaaa ggagtatttc tttctatgaa gttaactgat aaacaagtag ctaatatagc
     4261 ctttatccag caacttgaga ttccattgac acaaaaagat gaagaacaaa tagacttgat
     4321 ttttactgac acaaaggcta atgtgcgaca aatcaagatt actaaaaatg agcagcaacg
     4381 aatttgcttg tcttattcac atgaagactt gctgtatcga ggattgttta tctgccttcc
     4441 acgtttttta gctcaaggag gagagatgcc atacattgaa gaaacctcga tcattgagac
     4501 cagttattca ttagatgctt ctcgtaatgg cgtgatgaat aaagagactc taaaaaaatt
     4561 catttcttac ttagcgttga tggggtacaa cacattgtat atttacacag aagataccta
     4621 tgaagtagaa gaccttccat attttggtta ccttcgcgga gcgtatacaa aagaagaagt
     4681 gaatgaaatt gttgaatttg cttctggttt tggcatagaa gtagttccat cgatccagac
     4741 ccttgctcac ttgacccaat ttttgaagtg gtatgctcaa caagaactga tggatgatca
     4801 aaatactcta ctgatcggtg aagaaaaggt ttatgcggca atcgaaaaga tgattattgc
     4861 ctgcaaagaa atgtatcaga cagagcgtat tcatttggga atggatgaag cctatcaagc
     4921 aggtctggga cgatatttaa gcctgtatgg gtattgtgat cggacaactt taatgattca
     4981 acatatggac cgtgtgttat caattgttga taaatataag atgaaaccaa tgatttggag
     5041 tgatttcatt tataagctat tagatgaaac cggtaaagat aggtattaca atcctgattt
     5101 taaactcaaa gcattagcag ctaaaggatt accagcagga ttaacctatg ttcattggga
     5161 ctatggttca gatgaagttt cctcctatga gaaagtaatt gataaccatt tgaaattttg
     5221 tgacattgat cattacgttt tagctagtgg tgcccatatc tgggggagac tagccccgaa
     5281 tcatgggaaa acagaagcaa ttgtttctgc agctgtaaaa gcttctaaaa ataaaggtgt
     5341 acgttctgtg atgttaacta cttggggaga tgaagggcag gaaacagagc attggcattc
     5401 cttaattggg gcattaaaat tagcagatta tgtctattct gaagaagagt ggaatgattt
     5461 tgaggaaatc tacgatcgtt tattaggaac aggtagttat caattaatgt ccgaattacg
     5521 atatttcgat gaagtggata ccattacaaa taacaactta caaatggctt ccgtgagtaa
     5581 actgctactt tggcaagatc cgttagttgg tatatatgac tttcatgtag ccgctcataa
     5641 caaaattgcc actagaacaa ttggtactta ttatcatgag ttagctcaga aattagatat
     5701 gaataaaaat tggaatccaa aacaaccaat tttaacaact attcgtcaac gatatatact
     5761 tttagctgaa attttagcac aaaaggcaga actaggcatc tatttacaac actgctgtct
     5821 ggtagaagac cttgcaggat taatggaatt agcgaatcgg tgtgaagcac tagccaatcg
     5881 agtaaatgaa ttggcatcta ttcatgaaat aatttggtat caaacataca aaacctatgg
     5941 ttgggaagta ttagaacaac gatatgctgc attagctcat cgtctgaaaa ccgttgcgcg
     6001 tcgtattaaa aatcatcttc aggggcaaga tgccctagag gagcttttgg aagaacgatt
     6061 accgttttgt caatatcaag tgccgcttga aatttctggc tttaattatc ggcaaagctc
     6121 ggtgtcaggc tacaattaac gaaggaatct tcccgttgaa cggaccactt gctcatctaa
     6181 tgggaaagtg gttcgttttc tattcataag tagcttacca ctaactcttt tattaagaag
     6241 agaaaaaaag taggaagtga gaaaatatgt tatcggacta ttatcaaaat ctgtcagtta
     6301 taagtgtcaa tacagaacca ttacgtaatt attatattcc ttatgcaagt aaagaagaag
     6361 caaaacaaga gttagaaagg gaaaaaacga gccagtttat cgatctcaat ggtacatggg
     6421 attttcagta ttatccttct gtccaacagt taccggaaaa tttttttcaa gaggccttta
     6481 ctgaaagcta tgatagaatt tctgttcctg gagtttggca aacagaggga tttgacttac
     6541 ctcagtatat caatgtatct tatccaattc cttttgatcc gccttatgta cctaatgata
     6601 ccccttgtgg tgtatatcgg cgtcagtttc aaattgagca aacagatgca cgcttttact
     6661 taaatctaga aggagcagat agtaattgtt ttgtttggat aaatagaaac tttgtaggct
     6721 atgaccaagt gagtcatagt acatctgaat ttgatgtgac caagtattta caggttgggg
     6781 ttaatgaaat tcaaattcta gtgatgaagt ggtgtgatgg tacctacttt gaagatcagg
     6841 ataagtggcg aatgagcggt ctatttcgtc cagtgtatct tcttcaacga ccaactatgc
     6901 atctgacaag ttatcaacta cggacgttga ctgatcctaa aaaaggaagc ggaacattaa
     6961 tggtttcttt tcaacgattt actcctattg aagtatcgtt acctatttct tacgaattat
     7021 tccatcctga tggacgtctc atagcgaaag gggagtgtta ctcggaacaa caaataaatt
     7081 tggatgttga agattgcctt ttttggacag cagaaacgcc taaattatat acattacttt
     7141 taacatgtaa tggtgaatat tttaaagaaa aagttggctt tcgagcactg acgatcaaga
     7201 atcgtaagtt attgctgaac ggaacaccta ttaaattata tggagtcaac tatcatgacg
     7261 tgaatcagca aggcacgcca gtgatgaccc gagagcagtt acgtgcagat ttgttattaa
     7321 tgaaaaaatt aaatgtgaat actgtacgaa cggcacatta ccctaaatca ccagattttt
     7381 atgaattggc ggatgagatt ggtctgtatg taatcaatga agcagatttg gagtgtcatg
     7441 gtgtggttaa tctatatgat actgaagcga attatcaact aattgctaat gatccgacat
     7501 tcgaaaagcc gattatggac cgagtgatcc gttctattct tccttatcaa aatcgaagct
     7561 ctatcattat gtggtctatg gggaatgaat ctggttatgg agtaaatttt gaaaaagctc
     7621 aagctttagc acggaaattg gatgattcac ggttgattca taatgaacgc tcgattgaac
     7681 cagttattga ccaagtgaat gattttagta acttggatgt tattagtcaa atgtatcccc
     7741 cactggaaga tgttcatcgt cattgtcaaa gaagtgatac tgaaaaacct ttttttatgt
     7801 gtgaatatag ccacgctatg ggcaacggtc ctggagatct taaaacgtac ttcgatttga
     7861 ttcaacagta tgattccttt cttggcggtt gtgtctggga gtggactgat catgcaatca
     7921 atagaggaac tttagaaacg ccatcttatt tatacggtgg tgattttggt gaaaaatatc
     7981 acgacggcaa tttttgcgta gatggtttat tgaccccaga tcggcaatta acagtaggtg
     8041 cattggagta ccgtaatatt cattcgccta ttcaactgat tgctgtagat gaggagctgc
     8101 aagaagtaac cttgagcaat ataactgctt ttacagccgg ttcagcattt aacctttctc
     8161 ttgaatggta tatagatgga aaaaaaacag tttatgaagt aatgaatcct ttcatacttg
     8221 agcctggcaa aagttgttca ttgcccatca cagtacctac aacgattcat ggtgctcata
     8281 cggttgctgt ttcgctatct gtttataaaa atgatgagct ttatggacag caacagctct
     8341 ttcttaaaaa agaaaaaatg agattgccaa gaactgctca tcataatgat gcaccacaaa
     8401 tttcagtaaa aggcacagag ctgataatca aaggagaatc ctttatttat atatatcaat
     8461 tagcaaaagc tgcgttcact tctgtaaaga tcaatcaaaa ggaacttttg gaacgtccta
     8521 ctcaattttc aatttggcgt gcaccaacgg ataatgatcg taacttagta aacaaatgga
     8581 aagcagctgg atatgctgaa atgtctttag aaatcagaaa aaaagactgg aaattcgtag
     8641 atgatcatct cctgatcgaa acgtcttatc gaggagttat tccaggatta cagccaattt
     8701 ttacaatgca catacagttg aaaataaatg gtgcaggcgt aatcaaccaa aaagtatcag
     8761 tggagaaaaa tccgattttt ccagatttcc ctcgctttgg gatggactgg ccgttgaatg
     8821 atactttttc gaaggtttct tattgtggct atgggccgta tgaaagttat cctgataagc
     8881 actatgctgc gatgtttggt atttatgaga tggagattga ggaatttgat cagccatata
     8941 tcaaaccaca agaatatggt aaccgctggc aatgtgattg ggtatcaatc aaaaatcatc
     9001 aaggatctac catgacggtt tatggaaact ctaatttttc tgctctgcct tatagtcaag
     9061 ctcaattaac cgaaaagtca caccattatg aattaataaa agaaaataga acttggttca
     9121 atttaagtta taaacaaaat ggtatcggct cacacagttg cggtccttat ttagaagatc
     9181 gtttccgttt tatggaagat acttttgact ggaatatagt gattactttc aaaggagaaa
     9241 atagatgaaa gaaacaaaga tcaatcaagt caaaaaaaaa ttatctcgtt atcaagaagt
     9301 tgtttatcga aaccattgct taaaatggtc aaaaaataag ctgaatgttc gttttaatgg
     9361 attattttat cgtttcgaac agttagaaga agtctggaaa ccggtaacga atttccaatc
     9421 atgtcctgta cagctcataa atgatccaaa agaaacatac gatttatgta taacacacct
     9481 ttataaccag catttgaaaa atgacggatt ttatttaaag gttactaatg acaatcaact
     9541 tacgattgaa tcagcaaacc ttcgagggtt tcaatatgct atggaagcct tattagagag
     9601 ttttttttcg gtaggagatc aattattatt accggaattg attctgaaac atgaaccatc
     9661 agtgaaaatt cgaggaatca ttgaagggtt ttatggtatc ccctggacac atgaaatgcg
     9721 cctctctctt ttttcatata tgaaagataa ccaattgaat acttttatgt atgcccctaa
     9781 agatgatgaa ttattacgta aaaaatggcg tgaactctac gatgcgcaag aattagacaa
     9841 atttaaggag cttttatctg ctgctgaggc tagcaatatc gatttttggt acttaattag
     9901 tcctggaaat gatattgata ttacgtgtga agaagacatt caagttcttc tgaaaaagtt
     9961 agaacaactg attgagcttg gcgtatttca atttggtcta ttaatggatg atattgacta
    10021 tcaattaaaa ggtgctgcta aacgccgttt tcgagagcca gcattcgctc atgcctattt
    10081 agtgaatcga gtagaggaat atcttagcac tcggttgtcc aatcatgaat tagtcatttg
    10141 tcctacagaa tatgataatc gtcatggttc tcgctattta gcaacgttaa gtcaagaaat
    10201 accagaacaa ttaccgttct tttggactgg cccaagcacg ttagcggcgt caatttctac
    10261 agaagagctg caggaaatgg cttctgtcta tcaacggccg atgctgattt gggataatat
    10321 tccggtaaat gattatttag aggataaaga actattgttc atgagtccat atgagaatcg
    10381 aacaccgaat ctttctaagg agagatacca ggttacagga gtagtttcga atccaatggc
    10441 tcaattagaa gcttcaaagt ttacgatcaa tagtatggct aattacttat ggaactgtga
    10501 gcgttttgat cctttggaaa cttggacgtc agtcgttgaa aaagtagttg gaagtaaact
    10561 acaaccagct tatcttacct taaccaatgc ttttccaaat cattacacaa gcagtctgct
    10621 ttcagatgaa gaattactgc tagccaaaga cccagaatgg gtcacaaaag aaatggctgc
    10681 tttagtccaa gcagtaaaat ggatccagca agaacaacat acatctagat tgagaacaga
    10741 attggagcct tggttacaag caatcacaga aagttggact ttctggcaag aatggcaatt
    10801 aaaaaaagat ccaaaagaag cagaagaatg gttggctaag aaaaaaacac accgcatagg
    10861 acgtgattta gtaactgccc atttagagca aattatcaaa agttagatat cttaaaaaat
    10921 aaaaagcaag aaaaaggaga acaaaatggt caagagatta attagtgcaa actacagtga
    10981 tgtcaagaaa atgacgtcaa aagagttgaa gcaatccatc aaagcgagtg aaggacgggt
    11041 gattgtttgt gaaacggttg ctaatactct tccacaatct ggagaaatta cgaatgcaga
    11101 agttgcgtca gcatttggtg cagatttgat tcttttaaat ggtttcgatt gttatcgtcc
    11161 tgcagttttt ggactaccaa atgtgacgat tgagaaggtt tttgatctac agcaaacgat
    11221 tgaaaacccg attcctaaat taaaagaact agtcggtcgt cctgtgggaa ttaatttaga
    11281 acccattgcc cctaaaacaa atatgatgag tgagaaagta gaaatcagcc aaggaagagc
    11341 aagctctgtt gaaacgatac aaaaagcaga agaactagga gtcgatttta tttgctttac
    11401 cggtaatcca ggcactggag taaccaattt agaaattact aaatccgtaa aaatggcaaa
    11461 aaagtatttt tcgggattga ttattgctgg gaaaatgcat agcgccggct cagaagagcc
    11521 agttgtgaat aaagaggcga ttatttctta tgcggaagca ggcgcagata ttttactagt
    11581 tccttcagta gggtctatcc caggcttttt agagcaagac ctaattgaag cggttcggtt
    11641 ggcaaaaaaa tatgatttgt taacgatgtc tgcaatcggt actagtcagg aaagtgcccg
    11701 accagaaaca ttgcggcaat tagccattat gaataaaagt tgtggtgttg atttacaaca
    11761 tattggagat gctggatacg ggggattggc accagcagaa aatattttcg aaacctcggt
    11821 tgcaattaga ggaatgcgac ataccatcaa ctgcgttagc cgctcgatca atcgctaaca
    11881 aaattaaata aaaaatggtt aaattgatta tttaagtggg aacatacttg agatatcagc
    11941 taacttctgt cactttaaaa aagggactgt gacagaagtg aaaatagaca aaaaatatcc
    12001 gaacaagaga ctaggatagc agaaaaacta tcgttttgca acgaggacca actggaccgt
    12061 aggagcaatt ctcttattgt tcggatattt cgcttgttca aggacttatg tctgtagtgc
    12121 ccttttttgg taggaaaaat ggtagggaac catatcattt ttaatgaaaa tcaagcattt
    12181 caaaagttga aaaacgttga tttatcggca ttccccatcc atattatatt tacgtcttat
    12241 tcccactcca ctgttgcagg tggtttgctg gtaatatcat acacgatacg gttaacgtgt
    12301 gcgacttcat ttacgatacg gacactgatt ttttgcaaga cttcccaagg gatacgggca
    12361 aagtcagcgg tcatgccgtc aatagaagtg acagcacgga tacctacagt gtagtcatac
    12421 gtacggccat ctcccataac cccaacagag cgaatgcctg gtaggacggt gaagtattgc
    12481 cagatgtcgc gatctaaacc ggcattggcg atttcttcgc gcaagatagc gtctgattca
    12541 cggacgattt ctaatttttc ttctgtcaat tcaccaagga cacgaatacc taagcctggt
    12601 ccagggaatg gttggcgcca aacgatgctg tcaggcatgc ctaattgggt acctaattca
    12661 cgtacttcgt ctttgaacaa tgtgttcaat ggctcgatca gcttgaattg catgtcttca
    12721 ggtaatccac ctacattgtg atgtgatttg atggtttggg cagtttcagt tcctgattcg
    12781 atcacatcgg tataaagggt tccttgtgca aggaaagaaa cgccttcttc accggcaagt
    12841 ttggttgctt catcatcgaa gacatataca aattcatttc caatgatttt acgtttttct
    12901 tctggatcag acacgccagc aagttttgat aagaaacgtt ctttggcatc gacttttata
    12961 atgttcaagc caaattttcc gccaagactt tccataactt gttctgcttc acctttacgc
    13021 aacaatacgt gatcaacgaa gatacatgtt aattgatcgc cgatcgcttt ttgtaaaaga
    13081 acaccaacaa cagaggaatc tactccgcct gaaaggccta ataaaacttt tttgtcaccg
    13141 acttgttcac gaatttttgc gatctccatg tcgatgaagt tatccattgt ccagtcacca
    13201 gtacattggc agacatctag tgtaaaatgg cgcaacaagt catttccata ttcggaatgg
    13261 cgaacttctg catggaattg gattccgtac attttgcgtt ctgcattttg gatcgatgcg
    13321 atggggcaat ctttgctggt agcaacgaca tcgaaatcag tcggtgcttg ggtaactagg
    13381 tcaccatgac tcatccaaac ggtttgtttt tcaggagtgt tttggaaaag ggccgcttct
    13441 gcagaagtga tttccaattc tgcttttccg tattcacggt ttttggctgg tttaacagaa
    13501 ttaggcagaa gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg tatgagggtc
    13561 accgttggta actcgaagac cgacaactaa ggcgtattgc ctttttattt tgtcccatgt
    13621 tacaatcagt ctatacaaga aatgagttga tataacatg
//
LOCUS       DAZKCZ010000068        13653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000068 DAZKCZ010000000
VERSION     DAZKCZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.069"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..322
                     /gene="yaaA"
                     /locus_tag="ACG8KN_001804"
     CDS             80..322
                     /gene="yaaA"
                     /locus_tag="ACG8KN_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFP9188858.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            309..1433
                     /gene="recF"
                     /locus_tag="ACG8KN_001805"
     CDS             309..1433
                     /gene="recF"
                     /locus_tag="ACG8KN_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9188859.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1430..3376
                     /gene="gyrB"
                     /locus_tag="ACG8KN_001806"
     CDS             1430..3376
                     /gene="gyrB"
                     /locus_tag="ACG8KN_001806"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9188860.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3396..5867
                     /gene="gyrA"
                     /locus_tag="ACG8KN_001807"
     CDS             3396..5867
                     /gene="gyrA"
                     /locus_tag="ACG8KN_001807"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFP9188861.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6103..6402
                     /gene="rpsF"
                     /locus_tag="ACG8KN_001808"
     CDS             6103..6402
                     /gene="rpsF"
                     /locus_tag="ACG8KN_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9188862.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6452..6964
                     /gene="ssb"
                     /locus_tag="ACG8KN_001809"
     CDS             6452..6964
                     /gene="ssb"
                     /locus_tag="ACG8KN_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9188863.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6990..7226
                     /gene="rpsR"
                     /locus_tag="ACG8KN_001810"
     CDS             6990..7226
                     /gene="rpsR"
                     /locus_tag="ACG8KN_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9188864.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7369..9342
                     /locus_tag="ACG8KN_001811"
     CDS             7369..9342
                     /locus_tag="ACG8KN_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFP9188865.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9348..9800
                     /gene="rplI"
                     /locus_tag="ACG8KN_001812"
     CDS             9348..9800
                     /gene="rplI"
                     /locus_tag="ACG8KN_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9188866.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10074..11441
                     /gene="dnaB"
                     /locus_tag="ACG8KN_001813"
     CDS             10074..11441
                     /gene="dnaB"
                     /locus_tag="ACG8KN_001813"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9188867.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12221..13513
                     /locus_tag="ACG8KN_001814"
     CDS             12221..13513
                     /locus_tag="ACG8KN_001814"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9188868.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4551 a         2467 c         3003 g         3632 t
ORIGIN      
        1 tatatggata aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa
       61 tgaaaatgag ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt
      121 gggccaaatg ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt
      181 ggcagaaaac actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta
      241 tcccggcatg atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa
      301 cgacgataat gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga
      361 acatcgaatt ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga
      421 atcttttgga aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc
      481 aggaattgat tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt
      541 catccactct tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc
      601 atatcgaaca gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac
      661 cagaagatct ttctttagta aaaggcagcc cgcagaccag gcggaagttt ttagatatgg
      721 aattaggcca gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga
      781 aacaaagaaa ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt
      841 tggatatttt gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac
      901 agttcgtcaa gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa
      961 aagagcagct ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag
     1021 agcaaatcca agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc
     1081 ggggaacaac gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa
     1141 atgtgcagac ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag
     1201 cagaaatcga tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg
     1261 tgatgagcga gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag
     1321 ttcaaacgtt cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac
     1381 ctgagatttt ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga
     1441 aagaagttta gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg
     1501 acttgaagcc gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct
     1561 gcatcacctt gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc
     1621 aacgaaaatc catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg
     1681 ggggatacct gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac
     1741 tgttttacat gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca
     1801 cggagtagga tcttctgttg ttaacgcgct ttctacacag cttgatgtca aagtatacaa
     1861 agaaggaaat gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat
     1921 tatcgaacaa acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt
     1981 tacagagaca actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt
     2041 cttgaaccgc gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa
     2101 tgaataccat tatgacggcg ggattaaaag ttatgtcgaa tacttgaatg caaacaaaac
     2161 cgtgattttt ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt
     2221 ctctatgcaa tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca
     2281 tacgtatgaa ggcggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa
     2341 tgattatgcc cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga
     2401 cgtgcgagaa ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg
     2461 acaaacaaaa acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag
     2521 cgaacatttc atgaaattct tgctggaaaa cccaagtgtg ggccgtcaaa ttgttgaaaa
     2581 aggattacta gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg
     2641 taaaggtgct ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga
     2701 tcctgaaaaa tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca
     2761 aggtcgtaat cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga
     2821 aaaagcaagc atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat
     2881 gggtacaggg tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat
     2941 catgactgat gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta
     3001 tcgttacatg cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata
     3061 cggggtaaaa caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga
     3121 gttgaaacaa gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa
     3181 aggacttgga gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg
     3241 gatgatggcc cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt
     3301 gatgggggat cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa
     3361 gaacttagat atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca
     3421 tccatgatgt caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg
     3481 ttattgtcgc acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta
     3541 ttttatacgg catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca
     3601 gaatcgtcgg agatgtcatg ggtaaatacc atcctcatgg agactatgcg atctatgagt
     3661 caatggtacg gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa
     3721 actttggttc tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga
     3781 gcaaaatcgc tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca
     3841 actatgatga tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag
     3901 tgaacggaac aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt
     3961 ctgaggtagt agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat
     4021 tgatggaagt attacctgga ccagattttc caacaggtgg tttggttatg ggaaaatcag
     4081 gaatccgaag agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga
     4141 ttactgagat gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca
     4201 ataaagcaaa attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga
     4261 tcacagattt gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc
     4321 gtgacgccag cgcatctgtt atcttgaata acttatataa attgacatcc ttgcaaaatt
     4381 catttggatt caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac
     4441 aaattcttga aaactatatc gaacatcagc gcgaagtcat cacaagacgg acagtctttg
     4501 aaaaagaaaa agcagaagca agggcacaca ttttagaagg tttgcgtatt gctttggatc
     4561 atatcgatga aatcattgcg attatccgcg ggtctaaatc agatgacgaa gcaaaagcga
     4621 cgatgatcga acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt
     4681 tacgccgatt gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa
     4741 aactgattga agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca
     4801 agaatgaatt agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag
     4861 tcggtgaggt cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta
     4921 cattgacaaa caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa
     4981 ggggtggacg tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg
     5041 tttcttgttc tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg
     5101 caaaaggata cgaaattcca gaatacgggc gcacagccaa aggaatacca attatcaatt
     5161 tactaggtat cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag
     5221 aagaaggcca ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca
     5281 ctgcttttgc taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg
     5341 agctcgtcaa tgtgctactg acagatggtc aatctaatat cattattgga acacataacg
     5401 gctactctgt cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg
     5461 ttagaggaat acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaag
     5521 atcaagaagt attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat
     5581 atccagtgaa aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg
     5641 gtccactagc tggattgacc actgtttctg gagatgaaga catcatgctg attacagata
     5701 aaggtgtcat tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag
     5761 gcgtacgttt gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac
     5821 cagaagctgc agaacaaaca gttgaagaac aaggatcaga agaatagcca tcaagactaa
     5881 ttgtgtaagc tagcaagaat gaccgattaa aaaatctggt tattcctgct ggcttttttg
     5941 attagttgaa tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa
     6001 atagagtgta taatcagagc ttgtgagtag tctaattaat tagataactc tccttgctct
     6061 taggaaccta agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat
     6121 gaaatcatgt acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa
     6181 cgtttcgatg caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa
     6241 aaacgccgtt tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa
     6301 gtatcttctc catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac
     6361 gacgatatca ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa
     6421 caatagaaaa cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg
     6481 tctgacgaaa gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac
     6541 tttggctgtg aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa
     6601 ctgtgtgatt tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact
     6661 tttaggtgtt actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt
     6721 atacgtgaca gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga
     6781 aaatcgccaa caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg
     6841 aaacaataac tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga
     6901 tacttctgac ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt
     6961 ctaaacgaat ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac
     7021 gtcgtaaagt agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg
     7081 aattactaaa aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta
     7141 caggcgctaa aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct
     7201 tgctaccttt cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga
     7261 tcagctattc aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat
     7321 gacgaaagtt atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa
     7381 aacaatcatt tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg
     7441 gtagtcttaa ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta
     7501 tggggtgttc gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa
     7561 atccagcagg cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct
     7621 gtaggagtga ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca
     7681 ttgaaaatca aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact
     7741 gcaaaagaaa aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg
     7801 gatacagtaa agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc
     7861 catcagacga acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc
     7921 attgataaat tggatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt
     7981 tctgactgga tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc
     8041 ttcgtagcac agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat
     8101 cgctttcgag cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct
     8161 tatggctcaa gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct
     8221 ttagtccgtg gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt
     8281 tattatgggg ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg
     8341 agtaccgctt tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag
     8401 tttccggata tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac
     8461 cagaaaaaag cttacatcgt tatcaacgag aacgagatca ttccagatgt agaacggtgt
     8521 cttgaagaaa tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc
     8581 atagagctga aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta
     8641 tcgatctctc agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt
     8701 cgtggggaag agtttccaag taaacctctg ctgacttata tcgaatcttc agcttcttca
     8761 gcttcagagc tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat
     8821 cgttttgagg ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc
     8881 cgtacaactg cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca
     8941 tccttagtac aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg
     9001 attgctggaa gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa
     9061 gagtataata gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc
     9121 aatgcagctt tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt
     9181 accggcacga tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca
     9241 aacgcagcta ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct
     9301 gcaatcaaag aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt
     9361 ttttacaaga tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt
     9421 atgcacaaaa ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca
     9481 gtcagttagc aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag
     9541 ctaaaaagtt aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag
     9601 caggggaaga cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa
     9661 acaaacaata caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt
     9721 cactaggcta cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag
     9781 ttcatgtcgt acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag
     9841 aaggaaaaaa gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag
     9901 ctgcaaatcg atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa
     9961 gaaaacgagt ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac
    10021 ttttctctca ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg
    10081 aagtgtggca ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct
    10141 ctgtttttct tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact
    10201 tttatcgtcg tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg
    10261 aagcgattga cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg
    10321 ccggtggttt aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg
    10381 tctactatgc gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa
    10441 caggaatcgt tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg
    10501 ccgaacgcag tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg
    10561 ctgaagtact gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa
    10621 tcacaggtct accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg
    10681 aagaactaat cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata
    10741 ttgctcaaaa tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg
    10801 gggcagaatc tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt
    10861 tacgtacagg tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc
    10921 tttctcgagc aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg
    10981 cgaaatgtcg gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt
    11041 tgcagttgat tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc
    11101 gccaactgaa aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat
    11161 ctcgtggagt agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg
    11221 gatcaattga acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa
    11281 gagaaggcgg agatgacgat gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca
    11341 tcatcgagaa gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat
    11401 acaataagtt ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt
    11461 gagcaatccc gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt
    11521 acttaattta aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt
    11581 ccgaataaac ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat
    11641 gaggaactat tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc
    11701 tcttttcctt aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa
    11761 acatgagaag cagttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac
    11821 aacctctttc ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg
    11881 aaaaatatga gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt
    11941 tcacaacctc tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt
    12001 cacaaaaatt tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt
    12061 ctgttacaac ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt
    12121 tttataaaat aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata
    12181 caatggttca ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt
    12241 aggtacgcaa tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc
    12301 tgaagtgatt gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg
    12361 tgtgacttat aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt
    12421 gatcggaaac ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca
    12481 tgaaaaaggt gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc
    12541 ttatcatatc aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac
    12601 aacgattaaa ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat
    12661 tgcagactta ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa
    12721 aaaccgtcaa tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga
    12781 agaatattat gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt
    12841 gaatgatgct ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct
    12901 ggatatcgac cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt
    12961 aacaatcgga agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc
    13021 ctatacgtca cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga
    13081 acaaatccgc gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg
    13141 ctggtttgat tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc
    13201 tttaaactcg atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga
    13261 attagatggc gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa
    13321 accaatctat gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc
    13381 agaactgcca gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg
    13441 tatctcaaca ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg
    13501 ggcgcaaatc taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg
    13561 aagtgtttat ctatacgata ataataactt gaacactgtt tattcagaat aagggactgt
    13621 gacaaaaacc gtgtcacagt ccctttttcc gaa
//
LOCUS       DAZKCZ010000069        13146 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000069 DAZKCZ010000000
VERSION     DAZKCZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.070"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..149)
                     /locus_tag="ACG8KN_001815"
     tRNA            complement(77..149)
                     /locus_tag="ACG8KN_001815"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="ACG8KN_001816"
     CDS             530..1099
                     /locus_tag="ACG8KN_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFP9188869.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="ACG8KN_001817"
     CDS             1086..1946
                     /locus_tag="ACG8KN_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9188870.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /gene="yycF"
                     /locus_tag="ACG8KN_001818"
     CDS             2136..2840
                     /gene="yycF"
                     /locus_tag="ACG8KN_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFP9188871.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="ACG8KN_001819"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="ACG8KN_001819"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFP9188872.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /gene="yycH"
                     /locus_tag="ACG8KN_001820"
     CDS             4677..5993
                     /gene="yycH"
                     /locus_tag="ACG8KN_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFP9188873.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="ACG8KN_001821"
     CDS             5994..6863
                     /locus_tag="ACG8KN_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFP9188874.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="ACG8KN_001822"
     CDS             6918..7727
                     /locus_tag="ACG8KN_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9188875.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="ACG8KN_001823"
     CDS             complement(7830..8459)
                     /locus_tag="ACG8KN_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9188876.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="ACG8KN_001824"
     CDS             8629..9921
                     /locus_tag="ACG8KN_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9188877.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="ACG8KN_001825"
     CDS             complement(9997..10659)
                     /locus_tag="ACG8KN_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9188878.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="ACG8KN_001826"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="ACG8KN_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9188879.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="ACG8KN_001827"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="ACG8KN_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9188880.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattc
//
LOCUS       DAZKCZ010000070        13121 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000070 DAZKCZ010000000
VERSION     DAZKCZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.071"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..155)
                     /locus_tag="ACG8KN_001828"
     CDS             complement(<1..155)
                     /locus_tag="ACG8KN_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFP9188881.1"
                     /translation="MILIVKYRVEVLFMVTRIFILDDSLVFEKMLVEILEESRILLSP
                     WKFEIVQG"
     gene            complement(364..1200)
                     /locus_tag="ACG8KN_001829"
     CDS             complement(364..1200)
                     /locus_tag="ACG8KN_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFP9188882.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1211..2140)
                     /locus_tag="ACG8KN_001830"
     CDS             complement(1211..2140)
                     /locus_tag="ACG8KN_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9188883.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2332..2685
                     /locus_tag="ACG8KN_001831"
     CDS             2332..2685
                     /locus_tag="ACG8KN_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9188884.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2759..3415)
                     /locus_tag="ACG8KN_001832"
     CDS             complement(2759..3415)
                     /locus_tag="ACG8KN_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9188885.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3641..5575
                     /locus_tag="ACG8KN_001833"
     CDS             3641..5575
                     /locus_tag="ACG8KN_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9188886.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5661..6884)
                     /locus_tag="ACG8KN_001834"
     CDS             complement(5661..6884)
                     /locus_tag="ACG8KN_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9188887.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6878..8749)
                     /locus_tag="ACG8KN_001835"
     CDS             complement(6878..8749)
                     /locus_tag="ACG8KN_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFP9188888.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(8770..9888)
                     /locus_tag="ACG8KN_001836"
     CDS             complement(8770..9888)
                     /locus_tag="ACG8KN_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9188889.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(9971..10804)
                     /locus_tag="ACG8KN_001837"
     CDS             complement(9971..10804)
                     /locus_tag="ACG8KN_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9188890.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10797..11714)
                     /locus_tag="ACG8KN_001838"
     CDS             complement(10797..11714)
                     /locus_tag="ACG8KN_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9188891.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11941..13065)
                     /locus_tag="ACG8KN_001839"
     CDS             complement(11941..13065)
                     /locus_tag="ACG8KN_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9188892.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4102 a         2643 c         2166 g         4210 t
ORIGIN      
        1 ccctgaacga tctcaaattt ccaaggactt aataaaattc tggattcttc caaaatttct
       61 actagcattt tttcaaaaac caaactatca tccaaaataa atatacgcgt taccataaaa
      121 agtacctcca ctcgatattt tactatcaaa atcatttcat acaacttttc aacatacatg
      181 ttaggaataa attgtaattc tcttcacaat cttttccaac acctttgaaa ccgatatcaa
      241 atactttcag gatgctgctt ctaaaataaa tatcaaacaa atgaatacat catgcaaaaa
      301 aagccagaca accgacgtcc ttgcgtgtca gctgtctgag ctatgtttat ttattttgtt
      361 cttttaaaaa gaacgataca catactgatt tttcggagca gagactttct cagcgtttgt
      421 aacaactaca gatgggattt cacgcttgaa tggttgataa tagtctgatt ggatggttcc
      481 ttctatttta taccattcat catttttaac cgttactcct tccggcatat gcgtcaacaa
      541 accaaagact cctgaatctg ccacacaatg gataatgccg aatcggaaca agaagatatc
      601 tgtattgcta tcgttcggtg tttggtaaac aaatccttca tagctgattt ttttgccaat
      661 gaattcgcta gggtaattat aaattagctc cataacttct aaatagtttt catctgtgat
      721 cttgatagtt tgcttatccc catacttttc tttcaactca ttcatttggt ctaagtagtc
      781 tgatttgttg aaataaatac tggtatccgg ctttaagtat tgtgtctgca tctccggatc
      841 ccctactgat tctttactta ctgggaaatt aaatcctttg gcttctacga tcgtagtgtc
      901 gagactaact gttggaaaca acgtccccac gattactggc aaagctagca gtacataagc
      961 gattccacgt tgataaggtt ttgacaaccc atggtcatga tcgtgctcgt gtgccttttc
     1021 ttctgctttt ccgtttttga cccacaaata cagctgaact aaagccaaaa gagcagataa
     1081 aatcattgaa agaatagcta agtagcgata atggacattg atatattgat cgagtttgcc
     1141 agaaacttgc aagtacatca tcaattcgaa atacccagat aaaatcaaaa accgcagcat
     1201 attttccctc ctaaataaat aatgtgaatc caataacgct aagggcaatc agtccgatca
     1261 gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat cagtagattt ttgatatcga
     1321 ccattgggcc aaagaccaaa aaggcagcta caggtgctgt gccgaacaga cttaacaaag
     1381 aagatcctat aaacgcatct gcttctgagc aaagagacat cgtaaaggcc aataacagca
     1441 tgatgataat ggctaaaatc tttgttgagc cgagcgttag cataattcta gttggtaaat
     1501 aagtctgcat cgcagctgcg attaatccgc caatgatcaa ataacgacct gtatcaaaaa
     1561 attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg gatcagctga ttttttggtt
     1621 cattcttctc actttgttct tcttcatgga catgatgatg gtgattttct gctactttag
     1681 acgtgatttt ttctttcaat acagcgtctt tttgaaaata ggcaagccag acaccaatca
     1741 caagggcaac aaaaaagctg cctatcaccc gccagcttgc catctgccaa gaattgccaa
     1801 atgcgataaa agtggaaaaa atcacgattg gattgatgat cggagcagtc agcataaaag
     1861 caaacgctgt gtgaactggt acgccttttt taacaaattg atgaacgatc ggtacgatgc
     1921 cgcattcaca tgaaggaaag aaaaatccta agatcgaacc agtaaaaata gagaagatac
     1981 gattttcagg aagccatctc ttcacacgtt ctggggtcaa aaatacttgt aaggcgcctg
     2041 agataagaca gcctaataat acgaaaggta atgcttcaat aacgatcgat aagaaaatcg
     2101 tactcatctg caaaaccgaa tttggaataa aagaaaacat gagtttcctc ctttttattt
     2161 ctagttcatt cgctttgcaa atatacgctt ttattcaaaa aaaacaagtt gctctgtaaa
     2221 tttttacaca actcttattt tttaccaaag cacttcaatt gactttttct gttattccca
     2281 ttaaattaag gtctcataca tatcacttct atcataaaag ggagaataaa aatgaaacaa
     2341 accactaact tgactgaagt tcaagacata ctgcaacagt catccgttgc cgtcatttat
     2401 ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc cacgactaga ggaacttctc
     2461 acacattatg atattccggc acttcatttg gatgcatttg aaacaccaga agtagctagt
     2521 aaatttgaag tgctgactgc gccagttgtc ctgatttttc atcaaggaaa agaagtcttt
     2581 cggcaagcac ggttcatcga ctttaaaaaa atccgttatc tactagatga actgacagca
     2641 gaagatgatt ccctttctta tgaagaaatc ttccgaacaa aataatcgtt gtccaaacag
     2701 aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg ttcaatcgct ttttattttt
     2761 attctgctgt attttcggaa tgcgcatttg cgtcagaatg caagaaatag atcaaggttt
     2821 gcaattcatt cgtcaaatca acattttgga tcaatacatc tgacggagca ttcaatcgag
     2881 cagcggtaaa gttcaagatt cctttgattc ctgcttccgt taactgattg actacttctt
     2941 gtgctctttc agcgggtatc gttaaaattg ctacttcgat ttgttggatc ttgatttgtt
     3001 ctgacatatc ttccattgga taaacgggga taccgtctac gatgcgccca acgatttcct
     3061 cattcacatc aaaagcacaa ctgatacgta tgctattact ttgatggaat ttgtatttta
     3121 gtaaagcact tcccaagttc cctacaccga tcagcgcaac atttgtcaat tgatcgtcgt
     3181 ttaatgtctt tgcaaaaaag tgcataagat cttctacatc ataaccatac ccacgttttc
     3241 ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc actatctact tgaacggctt
     3301 cacttaattc agttgaagac acttttttct ttccagcatt atttaaaatc cttaaatagc
     3361 gataatacaa aggcagtctt ttagctgtcg cctttggaat aatatgatct ttcacgaaat
     3421 gacctccacg tattgtctaa ttacatcttt gatttctcca tcatagcagt atttatagga
     3481 aaaaatcaat tccattgctc agatattgtg aaattttatt ctttattcat ttatttatca
     3541 tttctgcaaa tctcgcagta taatggaatt tatggtagac tactaattga aaaatagttt
     3601 tttatttgag gagaattcac aaagtagaaa agaggattcc atgatattat tacaagccaa
     3661 tcagattgct cgccattttg gtgcagaagt cttatttgaa aacatacatt tagaaatcag
     3721 tacaggcgct cgtgtcgctt tagttggcag aaatggcgct ggaaaatcga ctttattaaa
     3781 gatcattgct ggaattgaag cacctgatga aggaaagatc gctaaaaata aaacagcgac
     3841 tctcggatac ctcgctcagg acacaggact tgattccaat gaaacagttt ggaacgagat
     3901 gctgaaagct tttgaacaag ttagacaaat ggaagagcgg atgcgcgagc tagagatttt
     3961 cattagcgaa ggtacgccag acacacctgc ttatagttct tatttaaaag aatacgaccg
     4021 gctgcaacac gatttttcag atcgaaacgg ctatggttat gaaaatgaaa ttcgttccgt
     4081 attgcatggg tttaaattcg attcttcatt ttatgatcag aaaatcgata ctttatcagg
     4141 cggacagcgc acacgtttag ctttagctcg catgcttttg caacaccctg acattttgat
     4201 tctagatgaa ccaacgaacc atttagatat cgagacactt tcctggcttg agaattatct
     4261 gcaaagctat actggtgcat tattgattgt ttcccatgac cgttattttt tagataaagt
     4321 agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc cattacaaag gtaactattc
     4381 gaaatatcta gatttgaaag cagaacagtt agcaagtgaa tggaaggctt atgaaaagca
     4441 gcaaacagaa atccataaat tagaagactt cgttgctcgg aaccttgttc gcgcctctac
     4501 cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa atggaccggc tggatcgtcc
     4561 tcaaggggat gaaaaacaag caaactttct attcagtata gaaaagcctt ctggaaatgt
     4621 agtcttgcaa atcgaagatg ccgctatcgg atatgataat gtcaccttat ctgaaccaat
     4681 caatctagat atccgtcgtc aagaagcgat tgctctagta ggtccgaatg gtatcgggaa
     4741 atcgacgttg ctgaaatcaa tcattcacca gatccctttt atccgtggaa aagaaacgct
     4801 agggacaaat gtcaccatcg gctattacga tcaaggtcaa gctgatctgc atacaaacaa
     4861 gacgatccta gcagaattgt gggatgagca tcctaccact ccagaaaaag agatccgaag
     4921 tgctttaggt gctttcttat tttctggcga agatgtagaa aaaccaatcc ctctgctaag
     4981 tggcggtgaa aaagcacggg ttgctttagc caagttagcg atgaacaaag aaaatttttt
     5041 gattttggat gaaccaacaa accatttaga tattgacaac aaagaagtat tagaaaatgc
     5101 attgatcgat tatgacggca cactgctttt tgtttcccat gaccgatatt tcatcaatcg
     5161 gatcgcgaca aaagtcgtcg aactctctga aaatggcagc aaagtctatc taggagacta
     5221 tgattattat ttagagaaaa agcaggaaga agaagaaatc gccgaattgt tggcacaaga
     5281 tgagccagaa tctgctcctg ctcccacgaa caaatctgat tattaccaaa gcaaagaaca
     5341 gcaaaagctt atccgtagcc ttcaacgaaa aattactcag gttgaagaag aaatggcgcg
     5401 gatcgatgaa tggatcgatc agttggaaca agaaatggtc gcgccagaaa acctggaaga
     5461 tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct gctagacaag atcaagaaac
     5521 aaaattgacg gaatgggaag aactaagctt ggaattagaa gaatatgaaa attaacgaaa
     5581 aaaccaaaac agaaaccatt catgctgttt tggtttttct ttttttattt tatcaaacaa
     5641 aaattccata tttacctttt tcaccaataa gaagaccaat tactagtcat tctcactaaa
     5701 gcttctaaat aatagtaatc accccaaata caaggtgtat ttaccccatt atttttgggc
     5761 ttattataaa cagactgtgt taaaaagcca tcttcttctt ccgttgtagc atatctttct
     5821 ttcaattgtc gcataatttt ccaaacagca gactggtata agttcttttc tgttttgtct
     5881 aatagacctg attctattaa ttctaataat ccacaaacca tgatcaccat actagatgta
     5941 tctttttctt ctctgctaga atcatcaaag atcaaatccc aataacaaat ggtatcttct
     6001 ggcaaccgat tcataaaata attggctaat attttgctta aatcaaaata tttttgttct
     6061 ttcaaataac gataacttaa tgcaaaaccg taaatccccc atgcttggcc tctagcccaa
     6121 caggaattat cactataacc ttgatgcgta cttccatatc ttggttttcc atctttgaca
     6181 tccatataaa atgtatgata agttgaacca tcttctctta ctaaataatc tgctgcattt
     6241 tgaatatgag cctgagcagc ttttttatat ttttgatttc cagtttcttc actcgcccaa
     6301 tacaataatg gaagattcat gcaacaatcc atgatcattc taccttgttg tgaaggatca
     6361 ctaggatctc cccaagcttg tataactcct gccttctccc aaaaacgctg aagtaatgtt
     6421 tctgcagcat caattgaagc ttccttcatt tttcgatttc ccgttatttt ataatctgct
     6481 acggttgata aagaatacaa aaaaccaaga tcgtgatgat tagtgataat ttgacagtca
     6541 atcctgtgga taaaatctat atctgctttt tcagctgttt gttgaaataa aggatcaccc
     6601 gtcttctcaa ataacaacca attaattcct gtccaaaatc cttctgtcca accaatattg
     6661 tctcttaatg gataacgtcc ttttatagga cactcgcttg ggaattgata ctgactgtta
     6721 ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac gctcgatctc attattccat
     6781 tctttttcca ttaattcttt ataacattca tttatccatt tttctggatg tattatctct
     6841 tcaaacatca ctttatcaag catttcccca ccagccttta gcataatttt agtctttttt
     6901 tattgttttt attttggata gttagcccgg ttgtcccatc aaaatcaatt ttgggacgat
     6961 tgttccatac ttctaaacta taatcccgat cttgatgagc aaccactcct gttccgagcc
     7021 aatgggttcc ttttccaaat gtaccctcta atccaggaat aaaagtgttt tcccatgtca
     7081 tcaagtttgt attgggcaaa cccataatag ctgtggattt tcgtttaata gtatcgtcac
     7141 ttaaacagat aatacctgaa aagccagctt cactcaccct atagttttcc ttgtcttctt
     7201 ctatgattat tccttctttc tcaggagaac attcaatagc aaaccctgtc tcatatagaa
     7261 catattctgt atctaattct actttgtgta cgcgaatctg ccagccttca aaaggaataa
     7321 gccaagttct gatttttgcc tgtaaagtag gttcccatgt agaggcaagt acctctttct
     7381 tacatacaat gtctcgtaca ttatttcgaa cgatgaactg tccataggtt ttgatgctct
     7441 cattcttaaa actggtcgtg atatatgtgt ccgctcgctg aatccctagc attgaatcca
     7501 ttgccatttc tgctttcaat tgattcgctc gcggtatcga aaaaccaaat tgactagaat
     7561 aagcaaattt taaatatttt tcattggtca atgtatggaa atttggccct aattgtcccg
     7621 cattcaaaaa aaatgtatgt ccattatcat gttgtatgag caaattggct tctgaaagaa
     7681 gtttagttcc ttcacgaata ggcataggca tttctttagc cgtccaatac tcatggtctt
     7741 caggtagcgc caacaataaa aaaatcttat tcagccataa tggagataac gtactattat
     7801 agggttcagt caatgtcaat tgagggtagg tataaccaat attcaggata cctttttcat
     7861 caaatatttc tttttgcatc caccatctta tattccgatt gactaacccc ttgatttcac
     7921 ctaatttaaa aggcaaaaga tcggcccaca acatagctga ccaaaagctt gaaactgcaa
     7981 aacgataaat catgcttcga ccaaaaggca catttgcacc atcttccgca aaaaaatgaa
     8041 tatagtctct agaaaaatca atagctcggt tcttatatat ctggctaatg ggcttttctg
     8101 gttctagtac gctataaatc aaaccataat aatgaaatgc aaaaggatta taataatctt
     8161 ggcgacctct tgaactgtct cgataccagc cattttgaag ataacaattt tctatattat
     8221 caaatgcttc tttcagtgct gattctttaa ctggataacc aaatttttgg ataattgttc
     8281 cgacaattat cttaaaaaat tgccaattac catctgcaca ttcatattca aaaatttgcg
     8341 agaaccaatg aataatctgg cttttttctc ctgtatctaa ttttttccat aataattgtt
     8401 catgatgata taacgctaat aacattgcgg gcatttctac catacgctgg tctctttcat
     8461 gtatttctcc ccaataatcg ggatgggaag gatctgttcc agcttttatt cctgatagaa
     8521 ttaatttcaa ccatcgttca tctaattggt caattgcggg ccccgctccc cataaaaccc
     8581 gagagaatcc ttccattcct gcaattttat ttccatatcc taccccttga tgtatataat
     8641 caatttgtgc gccacttgct cgataaaacg gctggagtgg ttgatttatc tttttaaata
     8701 agtcaaagta atcctgtctg gtccttaata gtggtactgg atgtttcatt gtattttctc
     8761 ctctttttac taccaaatag ctaattccga ctgttctaat cgaagtaagc cttcaataaa
     8821 aaaataatca gaatagatca gtggcacatt ataagtcagt tctctatggt atgcttctgc
     8881 tccgcctcca atgatcccat cttcatccat attccaattt gcatagtgct tttctgtctt
     8941 ttggagtata ttgatagcag cttcatgata catttttgat cgtacagagg aaacatatcc
     9001 tgctaattcc agaagaccgc atgcagcgca tactccagca ctggtatcaa tattcgaatc
     9061 ttttacagca aaatcaatga gaggaatata accagttttt tcgattttct gaataaaatt
     9121 tactgctatc ttttctgcta tagctagata gtacggattt tttgtgtaac gataacttaa
     9181 cgcgaatcca tataaagccc atgcatttcc tctgctccaa caagaattct cattgtatcc
     9241 ttgcccgcca agtaaccgaa tatatttccc tgattctgca tcaaattctg cgatatgccc
     9301 ggttgatcca tctttcctca ctagatactt agctgttgta tctgcatgct gccgagcaat
     9361 tttagagaac ctcggatcac cagtttccct cgaagcccaa tacagtaatg gtaaattcat
     9421 catactatca ataatcatcc aaccaggttt atggtcattc catgctacta aatagttccc
     9481 tgaaatatta tagcgtccag caagtaccgt agctgcatgt aaccctagtt gtcgactatg
     9541 tttgttttga gactgttgat aatgtgcaat agccgatggt aagaataaaa aacctacatc
     9601 atgatgaacg tctgaaaaat ggtacagggc agattctaat aattgttctt gttgtattgc
     9661 tgcctctttg aacttttctc gttttgtctt gtgaaagagc tgccataaaa taccactcca
     9721 aaaaccattg gtccaccagt cgattcctct gtcctttatt acgtctgtat attttccgtt
     9781 gtaagaaata tatggaattt tattccccat tctgtctaat tccgcatcta tcttttcaga
     9841 agctacattc aaaatttttc ttgaccattc cttcgtctgt tcattcatgg atatcccgcc
     9901 attcttttaa tttttatgaa tgtggagttg ctcctagaac gaactagatg gttaacattc
     9961 gttatttttt ttacttattt gcagctccaa tgtcattctc cttaaattta gactgcgccc
    10021 acacataaat caagaccatt gggatcactg aagttgctaa aattagcata attgcgccat
    10081 aattaattgg ctcacctgta ctagaaaccg tcatattttt gataattaag ggaaccgtat
    10141 atttatcttt atctgaaagc atgactaatg gaaatagata ttgattccaa cttcccataa
    10201 agataagtaa accactagta ataatcgtag gtgataaaat cggtacagcg attttcgtaa
    10261 aaatcaccca ctcattggca ccatcgattc tggcagcctc catcagtgct gtaggaaatt
    10321 gactgctata ttgtcgcatc ataaacactg aagttgtact ggcaatcgaa gggatgatca
    10381 cacctgcata agtattcgat aatcctaaac gactcataat gtaaaataaa ggaatcaatg
    10441 tggtaaaaaa aggcaccatc ctagatagca taactgtatt gaacaacagt ttattcccca
    10501 taaaacgata ctttgctaat gcataacccg ccattgtaga actggctgta gctaaaactg
    10561 tactgattac agcaataaca atcgaattcc aaataatcaa gggataatta tatctcgcca
    10621 tgatggcttg ccagtttttt aatagcatat ccccaaactt gaaattgatt ccgccactta
    10681 aaatttcttg attactttgc gttgaagcta caatcatata taaatatggc atcaaaaata
    10741 ttaaggcacc aaacacaata atgacgtacg taaaaattct gctttgtatc ttttgtttag
    10801 tcatttttat cacctccgaa tttaaattga atagccgata atataacaga tacaaacacc
    10861 atcgtccagg caatagcaga ggcttttcca aaatcaacca tttcaaaact tgttttatat
    10921 aaattgactc ctacgaccaa agctgaattt tctggtccat tggaagtccc aaagaatata
    10981 tttgggatat tgaaattacc caagccattg atagtggcta aaactgtggc cattaaaatg
    11041 attggcttta gcatggggat cgttaataaa aagaattgcc gaaatttcga tgctccatct
    11101 atttcagcac tttcgtatat ctctttagaa atcccttgaa gaccagctaa aaataaaata
    11161 gtgttatatc ctacactaag ccaaatcatc gtgatgatca ttgatattcg tatccaaaaa
    11221 ggatttgtta accactgtac accgttaatt cctagcgcat tcaaaaatcc attgaataac
    11281 ccagtcggat tgaaaaaagt catgaatacg gaagctacag ccaccgttga tgtaatggtt
    11341 ggaatataat agacagttct aaaaaatcct tttcctttta actgtgctga attcaataaa
    11401 acagcaaagt aaatagctaa tagcatcgtt attggaattg atcctgccat gataatcaaa
    11461 acattcgtgt aagatttaat aaacaatgga tctcggaaga tagctagata attgtcaaat
    11521 ccaacaaatt caaatgagta gccaattttc ataaaactaa aagcaaacga gataatgatg
    11581 ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg tgaccaatcc ataagcactt
    11641 tttttatgag acgtatctgt tgcttttttc ttgctgttct ttattgtctt gcttggtaac
    11701 gaagattgca tcatcgttaa gtcctcctaa tattttttaa tcaccagtta attttacgcc
    11761 gtacttatga gcaaaatttt ctgcttcttt tagatatgaa ttttcaaaat cacctgtttg
    11821 ccaatagtta tatgcagcgg tagtcaaata agacgtagca tctgaataag tggctggtaa
    11881 aacagtattt gatgtatttt cattgatttt attcaatagt tcatagtatt tttgtcctcc
    11941 ttagtagtca aactttttct cagctgcttc tgtttgatag gccaatgagt tagatgtagc
    12001 cacgcccagc tccagtgcgt tggctaagca ttcttcatct gtcagcataa atgtaagaaa
    12061 ttgttgtgcc acttgtggat ttttggcatc tttagggaca taaaaacttg atcctcctga
    12121 tactgcacct tttcctggat tttcttttga aaaaggaatc agtggagcca tgcgccattt
    12181 gtcagatgca tcaggaaaat tcaaagacat attcgtagca taccagccgc cagcaacaaa
    12241 cattgcagat tcttgaaatg ctttctcagc atcttgtgca ccgtagaaat gtacaatatc
    12301 tgagtcgatc atttctttga ttaactttgc agcatgtttc gcttctttag tattcagacg
    12361 gatatttcct tcttcatcaa gcaaagattg attctgctgg gctagcatgt tgataaacaa
    12421 ttcttggtcc ccagattctg gcaaggcaat tccaaaacta ccagtttttt cttttaatgc
    12481 ttttgccacc tggatatatt cttcccaaga actgatttct tcaaaatcta cacctatttc
    12541 agcaaatttt tttggttgat aataacaaag tgctggggta aaatcactag cccaaggaat
    12601 aatcgtttca ttactcgttt gttgacgcaa aatattcaat ttttgatcat aaaactgatc
    12661 gccatatttg tcataaaatc catattcatc agcactataa aaagcatctt gaaatttttc
    12721 cagcacacct gctgctgaag tgtctgccat ggagataata tccggcatat cttcgtgtgt
    12781 taccaatttt ggggtgatca tttgtactgt tgctgttccc gaagctacat tcacaatttt
    12841 taagtcaata tttgctccag ttttgcggtt atagattttt gcagcatttt caacatagtt
    12901 tgtcagtttg ccgtttgcga catatacttc taatgtagtg gcttttttta tcattggatc
    12961 tgcgatgttt gctgttactt cttcgttagt ttttccacat gcacttaagc tgaataatag
    13021 ggtaatgttt agcaaagcta tcaccaattt gtatcttttt ttcatgtcta tcccttcttt
    13081 cttttcaagt tcttatataa aacgctttca cttatgtata t
//
LOCUS       DAZKCZ010000071        12681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000071 DAZKCZ010000000
VERSION     DAZKCZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.072"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(238..600)
                     /locus_tag="ACG8KN_001840"
     CDS             complement(238..600)
                     /locus_tag="ACG8KN_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188893.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(674..1054)
                     /locus_tag="ACG8KN_001841"
     CDS             complement(674..1054)
                     /locus_tag="ACG8KN_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFP9188894.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1136..2311
                     /locus_tag="ACG8KN_001842"
     CDS             1136..2311
                     /locus_tag="ACG8KN_001842"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP9188895.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2388..2972)
                     /locus_tag="ACG8KN_001843"
     CDS             complement(2388..2972)
                     /locus_tag="ACG8KN_001843"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9188896.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3173..4171
                     /locus_tag="ACG8KN_001844"
     CDS             3173..4171
                     /locus_tag="ACG8KN_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9188897.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4283..4792
                     /locus_tag="ACG8KN_001845"
     CDS             4283..4792
                     /locus_tag="ACG8KN_001845"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9188898.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4822..5481)
                     /locus_tag="ACG8KN_001846"
     CDS             complement(4822..5481)
                     /locus_tag="ACG8KN_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFP9188899.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5483..6247)
                     /locus_tag="ACG8KN_001847"
     CDS             complement(5483..6247)
                     /locus_tag="ACG8KN_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFP9188900.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6255..6932)
                     /locus_tag="ACG8KN_001848"
     CDS             complement(6255..6932)
                     /locus_tag="ACG8KN_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFP9188901.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6969..8354)
                     /locus_tag="ACG8KN_001849"
     CDS             complement(6969..8354)
                     /locus_tag="ACG8KN_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFP9188902.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8564..8648)
                     /gene="ffs"
                     /locus_tag="ACG8KN_001850"
     ncRNA           complement(8564..8648)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8KN_001850"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8671..9186)
                     /gene="tadA"
                     /locus_tag="ACG8KN_001851"
     CDS             complement(8671..9186)
                     /gene="tadA"
                     /locus_tag="ACG8KN_001851"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9188903.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9293..10150
                     /locus_tag="ACG8KN_001852"
     CDS             9293..10150
                     /locus_tag="ACG8KN_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9188904.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10231..10566)
                     /locus_tag="ACG8KN_001853"
     CDS             complement(10231..10566)
                     /locus_tag="ACG8KN_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188905.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10633..12003)
                     /locus_tag="ACG8KN_001854"
     CDS             complement(10633..12003)
                     /locus_tag="ACG8KN_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFP9188906.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12081..12617)
                     /locus_tag="ACG8KN_001855"
     CDS             complement(12081..12617)
                     /locus_tag="ACG8KN_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9188907.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3773 a         2574 c         2114 g         4220 t
ORIGIN      
        1 gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca ttactttgtg
       61 aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac atattttgaa
      121 aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt ttggaccaaa
      181 caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat ctgtatttta
      241 gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt gacctggttc
      301 ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag tttcaaagac
      361 aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca aactaatctt
      421 gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta aaccagtctt
      481 tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat ggaccaaaaa
      541 ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt tgcttgccaa
      601 aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca cattttcaga
      661 aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat gcttcttcta
      721 tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt ttattcgtga
      781 aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt cttaaagata
      841 aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact ggctgtccga
      901 cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa acatgaataa
      961 gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc cctgtcactt
     1021 ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc gcatttatca
     1081 ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc ttaagatgat
     1141 cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg acgcaattgc
     1201 tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg acttcttgtc
     1261 tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct ttggttatgc
     1321 cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac accatgctgt
     1381 ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt tagctgcagc
     1441 agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag tttatcctcc
     1501 attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt tgctagaaaa
     1561 aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag aaaacaatgt
     1621 aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga gcaaagaaga
     1681 attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta gtgacgagat
     1741 ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga ttgatcctaa
     1801 tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc tagctgcaat
     1861 caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg aaaaacattt
     1921 agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc aagcggctta
     1981 tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa atgttcaaac
     2041 agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc ctgaaggaac
     2101 ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat tggagaacac
     2161 tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc cttccggtca
     2221 tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag gacttaatcg
     2281 aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa aggacgagac
     2341 cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta ttctgaaatt
     2401 tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg ttgtacattt
     2461 gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt atgtcggaaa
     2521 tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg gaatccagca
     2581 ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat aaagaattgg
     2641 cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag gttgaacagt
     2701 tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc cattcctgtt
     2761 ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa aatcacgcca
     2821 tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg tgctagttca
     2881 gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt tggtcctttt
     2941 tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat ttgcatactt
     3001 cataataata ataacataat acctaacctc tcgtaaaaat actagatttg ttttctttta
     3061 tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc aaacattttt
     3121 gagaagcatt ctcaattaaa ttctgctata gtgtgaaaag ggaggaattt ttatggaaat
     3181 ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt tttacgcagg
     3241 aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg gtcaattagc
     3301 aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa taaaagcaag
     3361 tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat tccatttaaa
     3421 agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta caaataaagg
     3481 catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc agccaagaaa
     3541 attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt tcgtgaatga
     3601 tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt ctgctatcga
     3661 ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc gtcgcccgga
     3721 atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca acttgcttac
     3781 tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata tccggttaaa
     3841 aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg tcaattatgg
     3901 attctcttct aatttagaac atctttcttc ttggggactt gatagtacca gaaatgccat
     3961 tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg gtgacatctg
     4021 tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac ctactgcagt
     4081 gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc acagcacaag
     4141 cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca tttcaagtta
     4201 aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat agagaaagaa
     4261 aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag aaaaggcaag
     4321 agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg tcatgttttt
     4381 acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg tcaacagcgt
     4441 gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag atatattagc
     4501 agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg aaggccttta
     4561 cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga tcggctttac
     4621 gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa acagccacga
     4681 tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg ctgctgctgc
     4741 cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat aattcaacaa
     4801 aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt tcaagctatt
     4861 ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa ataagataaa
     4921 gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa cgggatcgaa
     4981 taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc gatcaaatcc
     5041 tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa acggacgaat
     5101 caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac tcgggatcaa
     5161 agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat aaaagtgatg
     5221 ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga agggattatt
     5281 gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta aagaagtata
     5341 atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt tgatcgtcac
     5401 accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat attccatcca
     5461 ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt ttcacatagg
     5521 tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat aaaacgagca
     5581 aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta tctccaacca
     5641 tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata atgatggctt
     5701 ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa gagatgactg
     5761 accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg ttcgttccaa
     5821 tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca taggtcagat
     5881 aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta aatccggcag
     5941 ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc ccttttccat
     6001 ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct ggagctacat
     6061 gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta gtcgtgttat
     6121 tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat cgtttaccgg
     6181 ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa tatcctttat
     6241 agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt tgttttttct
     6301 acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga ctctgttttt
     6361 cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt ctcatcgata
     6421 tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc ttttttaccg
     6481 ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta aagatagtct
     6541 tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg gaaaaatcct
     6601 tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg ggctagtaca
     6661 tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc ggacacaagc
     6721 cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac tagctcgcct
     6781 ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt cagttcttcg
     6841 gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt tttatgctca
     6901 acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt ttttattata
     6961 ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac acttcggata
     7021 aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa aaatggaaca
     7081 aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat tggcttgttc
     7141 ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac catctgccca
     7201 aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa gttacgattt
     7261 ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac atttaacaag
     7321 tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc ttttggaatt
     7381 tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg ttctttcacc
     7441 gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact cagagaaaat
     7501 ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag ataactattt
     7561 atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc tttggctgaa
     7621 tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac atactgcccg
     7681 tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct gaataaatga
     7741 tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat ttggaaatca
     7801 tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg taaatcttcg
     7861 actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg attataagta
     7921 agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt cgttgtaatg
     7981 attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa taagttatcc
     8041 aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt ggaatttcct
     8101 acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat cagcaagata
     8161 ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa atcacctaga
     8221 tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg ttgatccaaa
     8281 aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt atgtaaaatc
     8341 actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat aggcagcttt
     8401 ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca aattgtataa
     8461 gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt ttacagtact
     8521 ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat cagacacatg
     8581 gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag cccaccattg
     8641 tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc agtctttttt
     8701 taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca tcccacattc
     8761 gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt cctccaaaag
     8821 attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat aaacttctgc
     8881 tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg taacaaaaag
     8941 tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga ttgcaagcat
     9001 ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc ttccaatcac
     9061 ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt ctgctttctt
     9121 tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa gatttgtatt
     9181 taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata tgataaaaag
     9241 gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca agatgaaaat
     9301 ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag catcagaagc
     9361 ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag attcattatc
     9421 tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc aaaaaaaaca
     9481 cccatggtat cactatcttt ttaaagatgc ttcttgtgtt caagaaaaaa gctttcgttt
     9541 gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc aagatactat
     9601 tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag atgcgctctt
     9661 agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta tttttctatc
     9721 tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc attcacctgg
     9781 caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag agcaaaacaa
     9841 tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt actatacaaa
     9901 agatactgta aaagatagta agttgatcaa aagttacata gaactgatca aacaaaatga
     9961 aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct ctgctgcaaa
    10021 aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc aagaacagtc
    10081 tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat tgcttcatga
    10141 acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac cccttatttc
    10201 gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa aatgcattag
    10261 ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag agattttctt
    10321 ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac tatctgaaag
    10381 accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga cgaatttttt
    10441 tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg atacaacgac
    10501 tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt ttgttgatgt
    10561 tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa aaaaataaac
    10621 gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta ctgttccttt
    10681 tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc atccttgatt
    10741 cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt gagtgatcgg
    10801 caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc taggttcaaa
    10861 tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta aaccatcgag
    10921 agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa gtcttccagt
    10981 agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct cttttgtcat
    11041 ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt gtccatgaaa
    11101 agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata tacgtattgt
    11161 tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg attgagtcaa
    11221 aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga tctcttcatt
    11281 ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt ttagcatttt
    11341 tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag attcttctgt
    11401 ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac aaggagcatc
    11461 aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg gtgcatgatt
    11521 tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa tcttcgcacg
    11581 tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag cagctaattg
    11641 ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg gttttgctgc
    11701 agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc cagcttgatg
    11761 gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg gagcgggttg
    11821 taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta gtggattcca
    11881 ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg atgcttcttc
    11941 gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg cttcctcctt
    12001 cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa ggtggctgat
    12061 ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc caagtattca
    12121 tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc ttgagaagaa
    12181 tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga cttttcttct
    12241 ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag cgaatccatg
    12301 tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa aaaatctttc
    12361 aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta gttttcaaaa
    12421 taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt gaaatgtgta
    12481 taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt aattgacttc
    12541 attccatatt cttccacgta taccatactt ttgaacagca aatattcttt actataattt
    12601 gttactctcc ccatcattct tacctccgtc tcttttcatc tacccataaa taaataacat
    12661 aaataattct aaaaatatta t
//
LOCUS       DAZKCZ010000072        12584 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000072 DAZKCZ010000000
VERSION     DAZKCZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.073"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACG8KN_001856"
     CDS             5..2377
                     /locus_tag="ACG8KN_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9188908.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     VPTDFLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VREIEATPEDKKRIESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3907
                     /locus_tag="ACG8KN_001857"
     CDS             2438..3907
                     /locus_tag="ACG8KN_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP9188909.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3918..6027
                     /gene="topB"
                     /locus_tag="ACG8KN_001858"
                     /pseudo
     CDS             3918..6027
                     /gene="topB"
                     /locus_tag="ACG8KN_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            6043..6192
                     /locus_tag="ACG8KN_001859"
     CDS             6043..6192
                     /locus_tag="ACG8KN_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188910.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6273..6869
                     /locus_tag="ACG8KN_001860"
     CDS             6273..6869
                     /locus_tag="ACG8KN_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFP9188911.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6882..7781
                     /locus_tag="ACG8KN_001861"
     CDS             6882..7781
                     /locus_tag="ACG8KN_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFP9188912.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7784..8269
                     /gene="ssb"
                     /locus_tag="ACG8KN_001862"
                     /pseudo
     CDS             7784..8269
                     /gene="ssb"
                     /locus_tag="ACG8KN_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            8314..8547
                     /locus_tag="ACG8KN_001863"
     CDS             8314..8547
                     /locus_tag="ACG8KN_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188913.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8706..8828)
                     /locus_tag="ACG8KN_001864"
     CDS             complement(8706..8828)
                     /locus_tag="ACG8KN_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFP9188914.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            8983..9242
                     /locus_tag="ACG8KN_001865"
                     /pseudo
     CDS             8983..9242
                     /locus_tag="ACG8KN_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9685..9891
                     /locus_tag="ACG8KN_001866"
     CDS             9685..9891
                     /locus_tag="ACG8KN_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFP9188915.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9891..10142
                     /locus_tag="ACG8KN_001867"
     CDS             9891..10142
                     /locus_tag="ACG8KN_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188916.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            10158..10595
                     /locus_tag="ACG8KN_001868"
                     /pseudo
     CDS             10158..10595
                     /locus_tag="ACG8KN_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10706..10942)
                     /locus_tag="ACG8KN_001869"
     CDS             complement(10706..10942)
                     /locus_tag="ACG8KN_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188917.1"
                     /translation="MKQNTSITKNTARFNCTAALSRISISPTNSTNNKGIILFKWITF
                     FLLSYRALPVYFAPPKRRGRLERLVRNKNHFLSY"
     gene            11040..11219
                     /locus_tag="ACG8KN_001870"
     CDS             11040..11219
                     /locus_tag="ACG8KN_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188918.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            11278..11634
                     /locus_tag="ACG8KN_001871"
     CDS             11278..11634
                     /locus_tag="ACG8KN_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188919.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         4642 a         2066 c         2619 g         3257 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccagag
      121 aaagaccaac ggtcaaccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agagcgaatc gaaaataaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 agtagaaaaa cttgaaagac aagaagagat aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgaga atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cggaaaaagg aaaagaaaac cttcttcctg aagtacaagc
      481 aatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccgaccattt attttctgaa
      541 aggctatcga aaagtcgctt tagaactgaa tgaaaacggg gaatttgagg aatctaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgt
      661 accaactgat tttttgaagg agaaagaaaa cgatcatttt tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcatactc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaatagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaaactt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgttggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctgtagaaac ggcagaacaa aagaatattg ttcctgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccacagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 ttcattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgagacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgctgaacta aagccgaaac atgaacggca aaatttttta gttggagacg accgtttaga
     2941 tcatgcggtt gcttttttat ggaaagatcc acagacagga gagacagtag gggcaagtta
     3001 tcaaggaacg atcgtcgatt ttaatcgttt cggtaaacgg ggaacgtata aacacattga
     3061 caagaatcct acgccaaatc acggatttaa tttaaaaata ggcgatccga agcatttaaa
     3121 attctttgaa agtagtattg atctgttaag ttacgctgca ttgaatcggg agaaactaca
     3181 agatgcctgg ttagtttcga tggacgggtt aaaacatcat gtaatcagtc attacgttga
     3241 agagtcgatt tctgaattaa gacgaaaaca aacatttcct caatcgattg aaatatgtgt
     3301 ggacaatgat cgtgcaggac atatttttta tgaaaaagaa cagatgaagg gaatcgttga
     3361 tccttttacc aataagaaaa tccgttgtga gcgaggaatt ccaaatgatt ggcaagtacc
     3421 taaagaatac aaagctactt atgaagcagt cgcaaaagaa atgagtgttg aacctgaagc
     3481 aattatggcc atacacaaaa ctgaaactaa tttacagctg acaaatcaat tggtgtctgc
     3541 tcatgatgtt caatctacgt ttggaaagat gttggcgaag ggcgaaccag tagaaacaat
     3601 tgatttgaaa gaagcatgta ccacggtcgc gaaggaactg aaagtatgtg aacgcgcaga
     3661 tggaacatac aactttgatc gtttttatag tagaaaagcc aacataaaag acgttaatgc
     3721 aggaatcctt ctttcctata aagcagaaca atactacaaa ggctataaaa agcatgaaca
     3781 tgaatttgta cctgaagtga aaaaggattg gaatgatcaa ttgaagcatg agatccagca
     3841 acaagaaatc agaaagcaaa aacgtgcgat gttgtttcaa caaggcagac aacaagaaag
     3901 ggagtgaagt cgtcactatg aaaaccgtca ttttagcaga aaaaccgtca caagcgaagg
     3961 cttatgccga tagcttttct aaagctaccc gaaaagatgg gtattttgag atccaagatc
     4021 gattgtttac aggagaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt
     4081 cgcccgatat gtacgatgaa aaatggaaac aatgggcatt agaacatttg cctattttcc
     4141 ccaatcaata ccactatcat gttcctaaag ataagaaaaa gcagtttagt gtcgtcaaac
     4201 gacagctgca gtcagctgat acgattgtga tcgcgactga tagcgatcgt gaaggggagc
     4261 tgattgcctg gtcaatcatt caacaagcag gcgccaatca aggaaaaacc tttaaaagac
     4321 tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccaacag ttacgagacg
     4381 cagaagagac ctatccaaaa tttgaagaag tacaagcacg tcagatcgcc gattggttaa
     4441 ttgggatgaa cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa
     4501 gtttttcttt aggtagagtc caaaccccaa cactctatat gatttatcaa ttgcaggaaa
     4561 agattagaaa cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa
     4621 atggcgcatt tgatgcgaag cttgatccaa atgaaaccca agcgacacaa gaagcatttg
     4681 aagcctattt aaaagaaaaa ggtgttcaat taggaaaaca gccaggtacg atccttcaag
     4741 tcgaaacaga gaaaaaaagt gcggctagcc cacgtctttt ttctttatcg agccttcagt
     4801 ctaaaatgaa tcaactcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt
     4861 tgtatgaagg aaaatatcta agctatccgc gaacggatac cccttatatt acggaagggg
     4921 aatatgcgta tctgttggat cacttagacg aatacaagca ttttttaaaa gccgaagcga
     4981 ttccgactcc tattcataca ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac
     5041 attacgcgat cattcctaca aaaacggtta tgacagcagc ggcttttgaa caactaagtc
     5101 cactgcagca ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa
     5161 agtataccta tgaagaaaca acgatcttga cgcaagttca gcagcttcag ttaaaggcaa
     5221 tcgaaaaggt gccgctagat ctaggttgga agaagttatt tggaaaagag agcgacggaa
     5281 aagagaagga agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtgg
     5341 atctacaagt tcttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga
     5401 tcactgcaat gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga
     5461 aagaagtgga aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac
     5521 aaaaggaata tatcaaggta gagaaaaata agctcgtggt gacgaataaa gggattttgc
     5581 tttgccaagc cgtggaaaaa gagccgctgc ttacgagcgc cgaaatgacc gccaaatggg
     5641 aaagctactt gttaaagatc ggtgaacgaa aaggaacaca aaccactttt ctagccaata
     5701 tccaaaaatt tgtctctcac ttattggaag tcgtaccagg gcaaatccaa tccactgatt
     5761 ttggatcgac gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt
     5821 taggaatttg tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt
     5881 gtacgtctga agcctgtgat tttaaaaggt cagaagggtt catttgaagc aacgattgtt
     5941 ttaaaagaag acttcactac aggctttgaa ttttctgaaa agaaaaaaac gaacttcaag
     6001 aaaagaacac gccgcactgc aaaataatgg agggttaatc ttatgcgtaa taccacacac
     6061 aaacaacaag aaatcgaaaa tcaaaaagcc aaaattcgtt cagaacagga aaaaatgcgt
     6121 gccagcaaac gaagactgca gcaattgaag aaccagcaga ggattcaaaa agaaatcgaa
     6181 ttagatcgat aaaatcttcc taactattgt atacgtataa acttatgtta tactgttttt
     6241 ataagtttat acgtatacaa aggagggaga aaatgacaaa agcaatggta tacaagctgc
     6301 tagaagaatt taatggaact ttggctttga aggatgccaa aaaagcgggc atttctcctg
     6361 taacgatcaa acgtatggta gatcgagggg agctggatcg agagtatcca ggttttttca
     6421 ccttacctgg tcaatttcct gatgaattgt ttatggcgca aaaaaaatat gaacgcggca
     6481 ttatttcaca tattaccgcg ttagatttgt atgatttaac agatatgatc cctagacaga
     6541 tcgatctgac tgtaccttac ggttatcacg tttctgaaaa aggattgaag gaattcgcag
     6601 ttgagctaca ctatacaaaa gcggaatggt atgaattagg taagattgaa ataaaaagtc
     6661 gctatgggaa tcctattata gcttatgatc cagaaagaac tttatgtgac atttggaatc
     6721 cctggtatac cgttgaggat gaaatcaaag taaaagcaat caaaaactat atggagtcgg
     6781 acagaaaaaa tcttagaaaa ttgaatgagt atcgacgaat tttaccaaca gacaaaacga
     6841 tgcgttctta tattatggct ttaaattaaa ggagaagata catgacacct acgcagttaa
     6901 aagcaaaagt gaagaatatt tctagagaaa aagaagttga tccgcagctt gttttaagac
     6961 attttatgat ggaaaaattc ttggaaaaaa tctcagagtc tccttatcgt gaaaattttg
     7021 tgttaaaagg cggcttcttg attggctcaa agtatggcat tgaaaatcgc acaaccaaag
     7081 atattgatac gactttacgg gaaatgaaag taaccaaaga aacattaaca acggttctaa
     7141 acgaaatttt ttcaacgccg acgaaagaag acattcaatt tgagattcaa ggaatgaaag
     7201 aaacgagaga agccgattac tatccaggat ttagcttacg tgtgctggca catttggaaa
     7261 acatgcggcc agattttaag gtagatgtga ctacaggtga ctcgatctat cctgcaacga
     7321 tcactcacag tcataaattg atgtttgagg atcgaacgat tcctttggaa tcctatccta
     7381 cagaacaaat tattgctgaa aaattaagtg caacctttga ttttttaaca gataattcga
     7441 gaggaaaaga tttttatgat ctctatacta ttcctaaaat ggagaagatc gatgagaaga
     7501 ccttgaaaga tagtgtgaga aatacattta cacgtcgagg aaaaactgat ccgttaaaga
     7561 tatactatga aaaggatatg gatgtactga aaaatagtca cgagctaaaa gatacctgga
     7621 agaaatatca aaaaagcaat ccttatgctg cgtcgatcac gtacgatgag acaatggatt
     7681 cgattagtaa tttgatggaa aaagtgattc acgtcgaaga aaaagagcga aaactagctt
     7741 atcaaagaca ccaacagatg aatagagaaa tggagagata aagatgatta ataatgtgac
     7801 attagtagga cgcttaacca aagatcctga tttacgttat accgcaagtg gtactgcagt
     7861 agcgactttt accctagccg tcaaccgaaa tttcacgaat caaaacggca attgagaagc
     7921 cgattttatt aactgcgtga tttggcgcaa accagcagaa acaatggcga cattagctaa
     7981 aaaaggtagc ttgattggtg tagttgggcg gattcaaacg cggacttacg acaaccagca
     8041 aggacaacgt gtatatgtga ctgaagttgt agcagataac tttcaactat tagaaagtaa
     8101 agcagcgaca gagagccgtg cgcacgctga tcaatcgtcc accagtccaa gcaccgccac
     8161 ctttgaacaa cggaatacag cgacgcctaa caacaatgga ttgaacgctt cacagaatcc
     8221 atttggtgga caatcaatcg atattagcga tgatgatcta ccgttttagt ttgagaaaga
     8281 aggagtaagc atggcaaaaa aattagtctg acgatggatc tattgatacc cgtaattgca
     8341 tttatagacg ggatttcttt gggcgtcact gaaaatcctt tggtaaatag aattgcgaaa
     8401 ggatcactat ttcttgtatt cgtgtatctt gtctgttctt atcttttaaa aagagcagga
     8461 aaggataatt ccttttggcg attgactaaa gtcacagtag cagcgacagc tgttatactt
     8521 acggctattc taccgcttta tatgtagagt taacgggaaa tcgttgaact tcttgcgaaa
     8581 tattgctata atgaacatga aaaagaggag ttgcgctaac aacccctcat taacagcacc
     8641 gattaaagaa cggtggcaga atattagtta tttggaaata accgctgacc tgctaaagta
     8701 gacggttatt tctttttgtc atttttaagc atatttacaa tcaaactaat caaagcaatg
     8761 gtaaacatac caaaaccaag aatggtttga attgtctcat atgcggacaa aaggcttact
     8821 cctttcatag attttagcac ttacattcat aagcaccacc acctttccag aagtcgccac
     8881 cgtcttcaaa ctttactgtc ttgactatta tagcatatct aaaatcgaat aaaaattctc
     8941 aattttagct gtttttgcta taattttagc gaggtgatcg tgatggaaca atatattggt
     9001 ttactcgata aattagggtg atcaagacac agccattgct tgtgcgattt acattacgca
     9061 cgagtcatga accaatcaat tgtgtagtgg ccaatataga aatagcggat aaattgctga
     9121 ttttggaaga aggcaagtat actatttcag tgacaggtca cttcaataaa cgaaagcaac
     9181 tagtcattga atcaatgcaa attagaaacc ctgatcattt tacgcgatcg atgagcatat
     9241 aaaagaagag ctgcagtcct aaatgaggat tgtagctctt tttgttttac atagaaacag
     9301 taaaaatgaa aaggcctgtt taggaaggca caagcgcctt ctcaattgaa agacctttct
     9361 ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt gaacgctacg cgttcactaa
     9421 gataaagggt cttgcatagc aagacaaaat agaaaaaatt tcctctcacc aaaaggttcg
     9481 ctcctgttcg ttatctcttt attgatttgt cagaccggca acaagttgcc ttttatcacc
     9541 aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc ccttgtcttg tcgcttttcg
     9601 ttgattcatt ctggccaaca aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt
     9661 tcctaagagg aggcagaatc aatcatgcga aagaaatttg attattgggg tgttcccttc
     9721 agcgaattat tcccaaacta tcatgcacca catactgtcg aatgcgattg tggagaaaga
     9781 gcgaaatgta tcaaaagcta ttgtttgtat caatgtccaa catgcgggaa aaaatatacc
     9841 ttgtcttatg gcgattatat tttaattgat gagaaaggaa agaaacgctg atgaaaacac
     9901 aacacaaatt attgactgta aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt
     9961 ctgaaaaagt agcagaagtt ttacttattc cgcggtactg gacgatagaa acccctttag
    10021 tagaggatgc accgactatg ctatttgaag acctagtgaa gattgcagga tatgaactgc
    10081 attatcaaga aaacttggaa acttatgaaa acgaatattg gttaaccaaa ttagagaact
    10141 aaaaaggaga aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg
    10201 ataaaatggc acaaaaaatg gaggatatga cgttcctata caacgaaaca cgtgttccaa
    10261 aaaaacatta taaagaaaaa ttgtcggtag ctgtggaaga aatgattgaa taaggtgtcg
    10321 aaatgaatct tattgcgacg tactatcgaa cactggaaga actgaaaaag caaaatgcaa
    10381 aatggttttt ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actattaaac
    10441 cttctgaata ccaagcttta gaactaacgt atgcgaaatt tatcgaaacg aaaaaagcca
    10501 agactgtttc aagtgaatgg ttagattatt ttgaaaatat caataagtat ggcgcatatt
    10561 acaccatgaa aaaggaagac aacgaaaatg aataaggtga tttatcaact aacaacggat
    10621 tccatatcaa cgatttgaaa gaaaaaaagg tcaataatca tttataccga aaagtgagaa
    10681 tgtgtgaaac aaggacaaaa aaagcctagt aactaaggaa atggttctta tttcgcacca
    10741 atcgctccaa ccgcccccgc cgcttaggtg gtgcgaaata aacgggcaaa gcccgataac
    10801 ttaagagaaa gaaggtgatc catttgaaca agattattcc cttattgtta gtactgtttg
    10861 ttggcgaaat acttatgcga gatagtgcag ctgtacagtt aaacctcgct gtatttttgg
    10921 taatactggt gttttgtttg atcttttggc ggtttgtatt agtcctcgtg agtctactat
    10981 tcaagatgat tgcagccata tttttcatag ggttatttcg atgaaaggag tgagtaccaa
    11041 tgccaattgc aataggaaat aaacggttac ccgtgacatt agatgaaaag agacaaaaag
    11101 aattgcagca actaaagcag aagtatggca aaagtgaatc taagattatg tgtattgcgt
    11161 tagatctatt gatcgcgcaa gaaaaagcag gatttgatgt accagcgcta aaaaagtgac
    11221 gtcacttttt aatttgatct atataagtga tatcactttt accaaggagg gaattaaatg
    11281 ttaaaaacta aaaagaattc gtacaaatta aagcttagta tactatctat tatttgtctt
    11341 ttaggcatga taattagtgg tcatttgtat agagtggcat atacgcagtt tattgagtac
    11401 aaggttcact ctttaggatt agctggggat cgatacgttg aatatgtcca ttattcatta
    11461 aaatgttctt catatattct tctttggctt attttttcat tgtttgaatt gctaaattta
    11521 atagcaatat cttcatatca cttagataag ctaaatacca aatctagccg ttataacgaa
    11581 gtgtttaagg aaaatgtgaa gttagaaagt gacttaaaca agctaaaaaa ataaaaagaa
    11641 gaaaacataa gttaactaaa attattttat taagaatgat ggaatataag gataaggtta
    11701 ccaacataac gatcattact tctctgatat gctatggact tctatcaatc agtattttgt
    11761 ctatagatta tggagtaact atactgcaag ccttagtttt ggctatgcct atgtatctat
    11821 ctttgtttat catgcatgaa atatataagc tatttgtttt atcaaaaaat gtagaaatac
    11881 gggtaaatat tttggtgaat agttatcaaa aagaactcaa agaaagaaga cggtggcggt
    11941 atttattgaa aggatgaaaa agtaggattt gacatactgg tattcagaaa gtgccgtcac
    12001 ttttaaaaca atggaaaagg tggagataag atgaagaaaa aacaaactaa actatcatta
    12061 gcagatattt aacacagtta accgcaactg aagatggtgt tgttgaattt gaacaatctg
    12121 aaattgcggt agtagatgat cgttacctta aaatgcctta cttctttgac caagcaaagg
    12181 tcatttgcct ttgtcactac gatggggtac gtgattttag gcgttaaaaa gtagtgatta
    12241 tatcaacttt acgagtaaat ataaatttag acatgaaact gaattatcca atggaaaatc
    12301 atggtttata atctagaaaa attatagtaa tgattggatt atatatattc tttcgtattt
    12361 tcgtacaact gtagcttgtc aaaattgaga aatttaaccg attaggtact ttacatcgct
    12421 atttgggtaa tttctaaaac atgaagctta ccaacgaata aactgagact atcttattaa
    12481 ttgatcaata taagaaaata tctttgagga gatgatgtac tatgttttcg tctatccacg
    12541 attggttagg tggtcttttc ggcggtggag gtaaaggttt ttaa
//
LOCUS       DAZKCZ010000073        12346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000073 DAZKCZ010000000
VERSION     DAZKCZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.074"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..459
                     /locus_tag="ACG8KN_001872"
     CDS             139..459
                     /locus_tag="ACG8KN_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188920.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            911..1111
                     /locus_tag="ACG8KN_001873"
     CDS             911..1111
                     /locus_tag="ACG8KN_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9188921.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1258..4047
                     /locus_tag="ACG8KN_001874"
     CDS             1258..4047
                     /locus_tag="ACG8KN_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFP9188922.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4094..4621
                     /locus_tag="ACG8KN_001875"
     CDS             4094..4621
                     /locus_tag="ACG8KN_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFP9188923.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4642..5832
                     /locus_tag="ACG8KN_001876"
     CDS             4642..5832
                     /locus_tag="ACG8KN_001876"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9188924.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5872..7170
                     /gene="asnS"
                     /locus_tag="ACG8KN_001877"
     CDS             5872..7170
                     /gene="asnS"
                     /locus_tag="ACG8KN_001877"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9188925.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8223..8897
                     /locus_tag="ACG8KN_001878"
     CDS             8223..8897
                     /locus_tag="ACG8KN_001878"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9188926.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8894..9235
                     /locus_tag="ACG8KN_001879"
     CDS             8894..9235
                     /locus_tag="ACG8KN_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188927.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9265..9624
                     /locus_tag="ACG8KN_001880"
     CDS             9265..9624
                     /locus_tag="ACG8KN_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9188928.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9884..10006
                     /locus_tag="ACG8KN_001881"
     CDS             9884..10006
                     /locus_tag="ACG8KN_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188929.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEKKKKE"
     gene            10148..12229
                     /locus_tag="ACG8KN_001882"
     CDS             10148..12229
                     /locus_tag="ACG8KN_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9188930.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         4110 a         2150 c         2668 g         3418 t
ORIGIN      
        1 aaaagaggtt gtgacgtggt cacgacctct ttttgacgat tatgatacaa aaaacatcat
       61 ttatgaacaa aatgtgaata acgggcgttc ttgagatatg attttaattg ggaaaaactt
      121 atcgataagg aggtctttat gaaaaaaaga ctgttatggt gtttgctgtt tctatcccta
      181 agtttaacaa gtcagccagt ttttgctgaa gaaacacagc ggtctcctat tacaggcaca
      241 cgtctgtaca aaaatgttta tgtacagaca acagatcttg attctgcgat tcctgaaaca
      301 gaagaacggt ctttgcagcc aagatttggt tacttttggt actgtccgac ctgtgattat
      361 gaaagttgct ggcatttctt acattctaca gctgcagctt ttgcttttac tcatactcag
      421 aaatatgatg gacatcatcc tgttatttat gaggtataga tatgagcaaa aaagaaaaac
      481 taatctcgtt gatcgtattg gtttgccttg ttcttctggt aactgtcata ggctatcaga
      541 aataccatga gaacatcaga cctactaaag aatcttcccc agcctcgacc gttgaaacag
      601 aaccgataag tagtcagaaa gaataatttt tttagtttaa agaagaacat cagagcaaaa
      661 aaacaccaac tgaacaatat gtcgtttatt ttaaaaaaag ttcggttttt cactttatgt
      721 aatcgctgag aaaacttttt ttaataaaaa aagtcgaaaa actctagctt ttttgtaaac
      781 ggtatgatat actataggag tagtttttgt tcggtaatct gtggtagctt gtttcaaata
      841 aacatcttcc agatatacac cattcaagta attgcaaaat atgtgttgat acacaaggag
      901 gatttttatt atggaaacag gtacagtaaa atggtttaac tcagaaaaag gatttggatt
      961 catcactgca gaaaacggaa acgatgtatt cgttcatttc tcagctatcc aaggcgacgg
     1021 attcaaaact ttagaagaag gccaagcagt aactttcgat gttgaagaag gccaacgcgg
     1081 acctcaagca acaaacgtta ctaaagctta attaacctat actaaaaaat aaacgcccag
     1141 acatttgcgt gtctgggcgt ttttgtctat tttctttaaa aagcgatgag agtatttttc
     1201 tgcactttgt agtataattt caaaagaaca ttagaggcag gagaagagga agatattttg
     1261 aaaccaagaa cttatgcagt cgttgatatc gaaacgactg ggacgaatcc aaaagaagat
     1321 cggatcatcc aatttggctg cgtcatgatt gaatcaggtc ggatcacttc acgtttttcg
     1381 atcgatattc atccagggcg gaagatttca aaacaaatcc agcatttaac agggatcacg
     1441 aatcaacgcg tccaaaaagc gccttatttc gaagatgtcg cccaaacgat ttacaatctt
     1501 ttggcagaca ccatatttgt cgcgcataat atttattttg attataattt tttgaaccat
     1561 gaactcatgc gatgcggact tcctagtttg aagataccag gcatcgatac agtagaactt
     1621 gcacaaattt tcttgccgac ggaaccaagc tttcgcttag ctgatttgtc agaaagcctt
     1681 ggatttattc atgagaatcc tcatcaggca gatagtgatg ccgaagtgac gggtcaactt
     1741 cttttgctga tcgaagaacg gatgagaaaa ttgccaatca tcacgttaga gcagattgcg
     1801 cgattaagta gacacacagg tatggatacg agtcgtttca tttatcatgt gattgaagaa
     1861 atgaaagaaa aaccagagcc acttgatccc tcattagaaa tcgtcgatgg gctagctttg
     1921 cagaaaaaag aagtggaact attcacatcc gtccattatg gcgagcggac atatccaaga
     1981 aaaaaacaag caaaagaaaa aatgtttggt aaaacactga tgtatcgaaa agaacagaac
     2041 agactgatga atgcggtcta cgaccatttc acaaaagatg aatcaaaaga tttgatgatc
     2101 gaagcagcta caggaatggg taaaacaatc ggttatctgc tgccattgag ttatttggct
     2161 acacctgaaa aacccgctgt catcagtacc gtttcgctag ttttgcagca gcagatcata
     2221 gaaaaagaca ttccattgct gaatcaattg ctagatcagc ccatacaacc tgtaattatc
     2281 aaaagctatc gccactatat tgatttacag cgttttaaag gcacacttgt ggaaccgcca
     2341 gaacaaaaac aatatgcttt gtatcaaatg gcggtcttag tttggctgac gcaaacgaag
     2401 accggtgatt tggatgaatt acatttaaca aatttgaacc attcattctt tgcagatatc
     2461 gcgcacagag gtattggatt tttagcaaga aatcagtcat tttacgaaca agactttgtt
     2521 cgtttcttgc aaaagaaaat caggcaaagt aattttttga ttgtcaatca tgcgttttta
     2581 atacaagaaa cacagcgaaa agaaccattg cttccagcta gtccattttt gttgatcgat
     2641 gaagtacatc atttgccgga aatcgctgaa aaagtaaatg acaaacgatt aagtattaac
     2701 tatttccata aacaaatcca gcaattagaa gaaccaaatc agctgtttga ccgaatacaa
     2761 agctatttac cgaaagaaca tgaagcctta cgctggttat cgatttatcg agaagaactt
     2821 tctgcgctat gcgaagcaca gatttgtatg gcagaaggat taatgagttt agtgagcggc
     2881 aagcaaccta acggacagta tccagaagaa gtcatgatga caaaagagat gttggatcag
     2941 ctgccactgg aatctgggac agccattgat cacctgcagc ttttttatca ggagatccag
     3001 cagctacagt cacaaatgcg tctgactttt gagattatcc aagaaagctg gacaaaccag
     3061 atgcggatcg atttagcaag tttatatagc ttatttgatc aattagacaa gcagtacttg
     3121 ctgatggatc aatggctgga agactggcga gatcatttga tccattggtt cgttcctggg
     3181 aacacagcgc agcagatgat ttttcatctc catgattttc aagcggctat gttgccgtcg
     3241 actgtttggt atcctcgata tgatcgtatt ctttatattg gaggaacgct gaagatcggc
     3301 cctaatcgac actatctctc taagcggctt ggcattgaag cagcacctct taaagtgatt
     3361 gcctctcctt atgattatga gaaacaggca aggatctatg tgccaaaaga ggcacctgcc
     3421 gtccatcaag tcagcaatca agcatatatc gattatttag agacagcact tacaggtctg
     3481 atcgaacaag aagatcggcc aatacttgtg ttattcactt ctcatgatgt tttgcagaag
     3541 gtctatcaga aaatgcatct gtctatgcta gaaaaaggac gagaaatact agctcaaggg
     3601 tttggcggaa gccgggagaa gcttttgaaa cgcttcattt tgtcagaaaa aagcgtattg
     3661 tttggcgctg acagcttttg ggaaggtgta gatttgcctg gggatgcttt gcagatcgtt
     3721 gtagtcacac gtttaccctt cgataatccg cttcgcccga tggtcaaagc taggaacgct
     3781 tatttagaat cagaggggat caatcctttt taccaggaat ccgtacctaa agcagctttg
     3841 aagttacgcc aagcattagg tcgcttgata aggacggaga cagatcgtgg cgtattgctt
     3901 attttggatc gaagactagt cactgcaaaa tatggtgcac ggatcatcca tgcattaccg
     3961 aaaaaagtac cgatcaaaga gctagctatg gaggaaatct atcaggacat ccatgaattc
     4021 ttaaagaaaa atgaagaagg aagttagagg agctgtcttt tttcataatt aaaatctgct
     4081 ataatgaaaa ggaatgaaga taaaggagcg acgacccttg aagaaaaaac agacaaagga
     4141 aaaaacgatt aatcgcgtgt tacttggctt gatcgtattt ttattagcgg tcatcgtgat
     4201 cagctcgatt ttttacttgc gttccagtcg tccggcggtg caggcgaaga aagaagcagt
     4261 agaaatcgca aagaaatatg caaatcttga ggaagccgat catttttatt ggtttactcg
     4321 aaagaagaca tacttttcac tgacaggtac aaatgataaa ggaaaagaaa tcgccgttat
     4381 cattcctaaa tctggtgaaa aagtaaaagt attggatcaa acaaagggac tgactgaaaa
     4441 cgaagcaaaa cagacaattg ctgcggccca tcctgaaatc caaatcgaaa aagctgcttt
     4501 agggatgtat gatgatcagc ctgtgtggga agtagtagga aaagaaacaa atgggaaact
     4561 gaattattac ctgattggct ttgaatctgg agatgaagtc aagacaatca agggaattta
     4621 aaaggtaaag gaatgaatgg gatggagtta tcaaaaagaa caaaacgatt agaaccgtct
     4681 gtcactttgg cagctgcggc aaaggccaag gcattaaaag caaaaggcag agatgtgctg
     4741 agtttgactg tgggagaacc ggattttgcg acaccagaaa acatccaaga agcagcaatc
     4801 gaagcaatcc gcaacgggaa agctagctat tatacaccaa cagcaggaat cccagagctt
     4861 cgacaggcaa tcgttgatta tctgaagaaa aactatggac ttgtctatga accttcacaa
     4921 acagtcgtaa cagacggagc gaaatttgct ttgtataccc ttttccaaac gatccttgac
     4981 ccgcaagatg aagtcatcat tcctgtgcct tactgggtca gttacggtga gcaagtaaaa
     5041 ctggcagaag gacttcctgt ctttgtgaaa ggggaagaat ccaatagctg gaaagttact
     5101 gttgaacagc tagaacaagc acgttcagaa aaaacaaaag ccgtaatcat caattctcca
     5161 tcgaatccga ctggaatgat ttatagcaag aatgaactgc aggcaatcgg tgaatgggca
     5221 gtaaaaaatg atattttgat agttgctgat gatatttatg gtcgtttagt ttataatggc
     5281 aatgagttta ctccaattgc aacgatttca gaagcaatca aaaatcagac tatcatcatc
     5341 aacggcgtgt ctaaaacata tgcgatgaca ggctggcgca ttggttatgc ggtgggaaat
     5401 cctgtgatca tcaatggaat gatcgctgtt gcttcccagt caaccagtaa tccgacagca
     5461 gtcagtcaat atgcagctgt tgaagcattg aaaggcgagc aagatacagt ggaagaaatg
     5521 cggaacgcct ttgaagaacg cctgaatacg ttatattcgc ttgtggcaga actgccgggt
     5581 gtttctcttg aaaagccaca aggagctttt tatctttttc ctaatgtaaa agaaacgcta
     5641 cgcatgtgta aatatgaaaa tgtcacacaa tgggtagaag atcttttaga agaaacaggg
     5701 gtagcgcttg tgactggcga aggctttggg gcaccagaaa atgtccgcat gagttatgct
     5761 actgatcgcg ctacgttaga agaagcggta cgcagaatca agcaatttat tgagagtaaa
     5821 agtcaaaatt aatcctaaag aaattcaagg aatataaaag gagagtcata cgtggaacaa
     5881 attcaaatca tcgattcgaa aaaccatgtg ggagaaacag taacaatcgg cgcttgggtc
     5941 gcaaataagc gatcaagcgg aaaaatcgca tttttacaat taagagacgg aacagccttt
     6001 ttccaaggtg tagttgtaaa aagcgaagtg tcggaagaag ttttccaaac agctaaaaat
     6061 ctacaccaag aaacatccgt gttagtaact ggagaaatcc gtgaagacag tcgttcaaaa
     6121 tttggttatg aaatcggaat tacaaatgtg gaagtggtaa gcgagagcca cgattatccg
     6181 atcacaccaa aagaacacgg aacagacttc ttgatggatc atcgtcattt atggctgcgt
     6241 tcttcaagac aacatgcgat tatgcagatc agaaatgaaa tcattcgtgc aacgtatgaa
     6301 ttctttaatc aacatcattt tgtcaaaatt gatccgccga tcttgactgg ttctgcacca
     6361 gaaggcacta cggatctatt cgaaacaaat tactttgatc aaaaagctta tttatctcaa
     6421 agtggccaat tatacatgga agctgctgct atggctttcg gaaaagtctt ttcattcggt
     6481 ccgactttcc gtgctgaaaa atcaaaaaca cgacgtcatt tgatcgaatt ctggatgatc
     6541 gaaccagaaa tggctttcac tcatcaagaa gaaagtttag aagtacagga acaatatgta
     6601 gcattcttag tacaaaatgt tttagatcat tgcgaatatg cattagatgt actaggacga
     6661 gacaaagaag tcttaaaaac ttatacaaaa ttgccattcc ctcgtattac ttatgatgat
     6721 gcgatcgaat tactgaagaa aaatggattt gatgacatcg aatggggcga agactttggt
     6781 tcacctcatg aaacgttcat tgcgaattca ttcgaacagc ctgtctttat cctaaactat
     6841 ccaaaagcca tcaagccatt ctacatgaaa ccacatccaa caagagaaga cgtggtgatc
     6901 tgtgcagata tgatcgcacc agaaggttac ggtgaaatta ttggcggaag cgaacgggca
     6961 acagactatg atttcttgct agaacaaatc cgtaatcacg gtttggatga aaaagaatat
     7021 tcatggtatc ttgatctacg taaatacggt tctgttccgc actctggttt tggtctaggt
     7081 cttgaacgaa cagtcacttg gattgctggt atcgaccatg tacgtgaagc tattccattc
     7141 ccacgtttgt tgaaccgaat ttatccataa gaggtgatga aaaagctgtt ctgcatcaag
     7201 taattagaac agccaagcaa aaacagctgt tcatgttttt gaattggcgg gacttattac
     7261 cgcagatgta agcttttgaa taccgtttag tggagaggtt gtgaaaaagc tgtttagttt
     7321 cgagtattaa gaaaaatttt gtggaaatgg ctttccacat ttttgcataa atttggctta
     7381 ataccgagaa actggctttt gaacaccgtt taaggaagag gttgtgcgcc tgccgttcag
     7441 ctccgagtca caaggagtaa tttcaaaaaa cagtttttca tgtttgatga ctattgtgac
     7501 ttgtgaccga ggagttggcc ggagagcacc gtttattcta aaaaagaact gcgaactctc
     7561 gcagttcttt ttttaaaata cgggataaag gaagtttcca gcagacaaga gaagaaagca
     7621 aaagaaattg ctatttgatt cacaaggttc tataacgaaa tcgcaatcaa tcaaacattt
     7681 acaaagaagg taattcaaat ggaaatctga tttgatgatg cttctgctgt tgaaataaag
     7741 cagcatctag cacctgaaaa caaactaata gtaacattcg aagatggagt aggtccgtat
     7801 tttcagcatg caataatcca tatgtaagtt caattcaccc ttgaaatcat cggaccagat
     7861 aaagttatga ccaatctatc ccaagcaatc tagaagagat attagtaaag ggacatttac
     7921 tagaagtctt agatgagcct ataaatatat gttctgatgg cgtcatcagt ggtctgattg
     7981 acggaagctt aggtgtgatc gatctctctg gaccaaacaa cgaaggaatc aaggggaatc
     8041 cggcacgtta agaaaaaaat agcataaaaa actagacact tgatgagcgt tcattcaatt
     8101 gtttgaaggg actaggctgg tgtcattttt tatacatata ttttctcttt cgtcttgata
     8161 gggcttatgg gataatataa ggaagaaaat catcgtttta ttcaaaaagg agtttttgac
     8221 aaatgaaaaa acgtatatta tttatcggtg atagcattac agacgcagga agagaccgag
     8281 cagaagcaac tgatctaggt aaaggttatc cacttttgat tgccggaaaa ctaatctccc
     8341 aatatccaac gatggaattt gaatgcatga accgtgggat aggaggagac aaaattgcag
     8401 atctgcaagt acgctggcaa gaagattgtt tagctttaca acctgactat gtatcgattt
     8461 tgatcggaat caatgatacc tggcataata ctagcgatca ggctatattc ggaactgaga
     8521 aatctgctca gcaatttgaa tccgtttatc gaaaattact aaatgaactt caaacagtgg
     8581 gaatctgcaa catcatcttg atggagccgt ttgtacttcc tataaccgac gaccgtcatg
     8641 gctggcgtaa agacttagac cctaaaatcc aaattgttcg taaacttgct aaggaatatg
     8701 gcgcattgct ggttccttta gatggtatat tcaatgcttt agggattgtt tatggttttt
     8761 cgaaatatac aagagaagat ggggtccacc caacgattgg cggacatgag atcattgcac
     8821 aaagctggat agatgcaatt gccgcagtcg atttttttag tggagataaa aatgagcgaa
     8881 agaaaagtga gaaatgatgg aaatacggtg ggcagtaaaa gaagatctac ctcaaatcaa
     8941 tcagctgatt actgatcatc cagaatcatt cgtaaaagca aaaacggcag ctgacctaaa
     9001 tgataagaat aaactattgc tcgttgctgt aacaaaagaa aaacggatta tcggatttgc
     9061 gcaagcggac gtgcaacagt cagaagaacg attggatcag cgaccatcag gaaaactaat
     9121 ccaacacaca ttagtaacaa aagaaaatac ggaagaaatc caaaaaaaat ttgcagaata
     9181 tttgatactg gaagctaaaa aaagaagaat aaaatcattt tttgcccagt tttaatcaaa
     9241 aacagtaata gaaggagaaa tacaatgagt tgtttaggaa atgtcatctg gtttatattc
     9301 ggcggactaa tcggtggtct tagctggtta gcagcgggga tactttggtg tatcacgatc
     9361 ataggtatcc caatcggtat gcaatgtttt aagatcgcct ccctttcttt tgcgcctttt
     9421 ggcaaagaag taatttatca ccagtctggc atgtcattga ttgttaatat catctggctg
     9481 atcgtttcag gcctaccaat tgctctcgca catgtcgtca gcggcatcct tctatgtatc
     9541 acgatcatag ggataccttt tgccaagcaa tctttcaaac ttgctcgatt ggcgttgatg
     9601 ccattcggtg cagaaattgt ataaaagaga ataataaagg ttagataaag catagagtca
     9661 ggtgggatac atgtatttca cctgactcta tgcgttctcc tgtataagtt cttccatgtg
     9721 acttttacgt cttttattta tatggggtta ctgtttatgg aaagttgaaa taattttcat
     9781 taaagaactc tttctatatt tttgaaagcg tagtggagct tcgaatatct gggaaggaca
     9841 ttttgcaggg attgaaaaaa gatattgtcc tatcgaaaaa ttaatgaatt tagacaaatt
     9901 gccagcaaca ggcttcaaac ttagttgcta tcctgtcaag atcaagaagg caagtgcggg
     9961 ttggataaga gcagtggcaa tgatcgaaaa gaaaaagaaa gaataagcca aaacatctgt
    10021 atggaagaaa ttttccgatg cagatgtttt tcattttttc tttcggatac cttgacatat
    10081 gaataaccgt tcatttataa ttagttatga agagatattc atatgttgag gcgaggaggt
    10141 cattaaaatg aaaagttaca gattagaagg tttagattgt gcgaactgtg caatgaaaat
    10201 cgaaaaagga gttcagaaga tcaatggagt caaggaagca acggtaaact tcacttctgg
    10261 aaaactaacg attgacgcag aggaagatca tttggctacg attgaacaag aaataaaaaa
    10321 agtagtgaag gaattagaac cagatgtcaa agtaacagaa atagataagg aaaaagtatc
    10381 tgaacatgga aatgaaaaag agagaaatac gctgttccgg attttatttt cccttgcagg
    10441 tatcgcttta ttgctattat ttgattttaa tgaaccaatt cgcttgatag ggtatttatt
    10501 gatttatctg ctgataggat atgatgtagt gaagaaggca gtgatgaata tcgtcaaagg
    10561 aaaaatcttt gatgagaatt ttcttatgtc tgttgctacg attggcgcta tgttgatcgg
    10621 tgaatatcca gaagccgtag cagtgatgct attttatcaa atcggcgagt atttccaagg
    10681 attggcagtc agtcattcaa gaaaatcgat tcgcgaattg atggcgatcc gtccagaaac
    10741 agctcacgtg cagacgacgg aaggattgat gaccgtgaac cctgaagacg tgcagatcgg
    10801 tcagtttgta cttgtgaagc ctggtgaacg tgtaccgtta gatggaacga tcatcgaagg
    10861 agaatcatta gtcgatacgt cagcacttac aggagaatca gtccctaaat ctgtatatgt
    10921 tggtgaaaca gtattaagtg gatttatcaa taaaaataaa ccatttttag ttcaagtaga
    10981 aaaatcttat gaaaattcta ctatcagcaa attattagaa ttagtagaaa atgccagcag
    11041 taaaaaagca ccagctgaaa atttcattac taaattcgcg cgctattaca caccagtcgt
    11101 tgtaggattg gccgttttat tagctgtttt acctccactt gttgtttctg gagcggcgtt
    11161 ttctgaatgg atctatcgag cattgacatt tttagtgatc tcatgtccat gtgcattagt
    11221 gatctccgta ccccttagtt tctttggcgg tatcggagga gcaagtaaaa tcggtgtatt
    11281 agtaaaaggt tcaaattact tggagttgct tgcacaaact gagacagtgg tattcgataa
    11341 aaccggcaca ttaacgaaag gtgacttttc gatccagacg atcgatacaa aaatcgatcc
    11401 aaagatattc atgcaatatg ttgcgagtgc agaacagttt tctacacatc cgattgctca
    11461 atctgtttta gaaggctacg caggtccgct tttaccaaca gctaatatcc aagaatttgc
    11521 aggtgaaggg atcttagctg aagttgatgg caaacaagta ttagcaggta atcacaaatt
    11581 gatggaaaga tttgaaatct cttttccttc aagtcaagaa atcggtacat tgctttatct
    11641 agcgatcgat caaagttaca gtggttatct tgttattgca gataccttga aagaagatgc
    11701 cgtagacgca ttggttcaat taaaaaaggc tggtgtgaag aacacagtca tgttgacggg
    11761 cgattcgaaa aaaattgctg atcatatcgg gaagcaagta ggcgtggaca agatatatag
    11821 cgaattgctt ccagaagata aagtgcagcg gttagaggaa atcttacaaa gaaacaataa
    11881 aaagaccgca tttgtcggag atggaatcaa cgatgcaccg gtgctagcaa gagcagatgt
    11941 aggaatcgca atgggtggat tgggaagcga tgctgcaatt gaagcagcag atgtagtcat
    12001 catgaatgat caaccatcga agattgcaga agcaatccat ttagcaaaga aaacattgaa
    12061 aatcgtcaaa cagaacattg tctttgctat tgggataaaa attttggtac ttgccttagg
    12121 tgcttttggt tttgcatcaa tgggagatgc agtatttgca gatgttgggg tgacagtatt
    12181 agctgttttg aatgccatgc gaagcttgca tgtaaaaaat aaacgttgat caatgaagag
    12241 gacgaatgaa cgtcctcttt tttttatatc cttttcttaa tgaaaaatgt aaagaaaata
    12301 gataggatta taaaataact aatgttttta attttgcaga aaattc
//
LOCUS       DAZKCZ010000074        12329 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000074 DAZKCZ010000000
VERSION     DAZKCZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.075"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..371
                     /locus_tag="ACG8KN_001883"
     CDS             <1..371
                     /locus_tag="ACG8KN_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188931.1"
                     /translation="IYYRYLDEFYRIFASKLSSILSDKINVKKSFFKKETPVEKISVE
                     LLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIATLIYS
                     VTDRHWQLVEDADLSYLPLK"
     gene            complement(452..1021)
                     /gene="thiT"
                     /locus_tag="ACG8KN_001884"
     CDS             complement(452..1021)
                     /gene="thiT"
                     /locus_tag="ACG8KN_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFP9188932.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1087..1189)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1195..2166)
                     /locus_tag="ACG8KN_001885"
     CDS             complement(1195..2166)
                     /locus_tag="ACG8KN_001885"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9188933.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2260..3633)
                     /gene="glmU"
                     /locus_tag="ACG8KN_001886"
     CDS             complement(2260..3633)
                     /gene="glmU"
                     /locus_tag="ACG8KN_001886"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9188934.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4136..4954)
                     /gene="purR"
                     /locus_tag="ACG8KN_001887"
     CDS             complement(4136..4954)
                     /gene="purR"
                     /locus_tag="ACG8KN_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFP9188935.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5121..5945)
                     /locus_tag="ACG8KN_001888"
     CDS             complement(5121..5945)
                     /locus_tag="ACG8KN_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFP9188936.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5918..6598)
                     /locus_tag="ACG8KN_001889"
     CDS             complement(5918..6598)
                     /locus_tag="ACG8KN_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9188937.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6621..7559)
                     /locus_tag="ACG8KN_001890"
     CDS             complement(6621..7559)
                     /locus_tag="ACG8KN_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9188938.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7804..8655)
                     /gene="ispE"
                     /locus_tag="ACG8KN_001891"
     CDS             complement(7804..8655)
                     /gene="ispE"
                     /locus_tag="ACG8KN_001891"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9188939.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(8809..11382)
                     /gene="mprF"
                     /locus_tag="ACG8KN_001892"
     CDS             complement(8809..11382)
                     /gene="mprF"
                     /locus_tag="ACG8KN_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9188940.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11523..12329
                     /gene="proC"
                     /locus_tag="ACG8KN_001893"
     CDS             11523..12329
                     /gene="proC"
                     /locus_tag="ACG8KN_001893"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9188941.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         3655 a         2681 c         2137 g         3856 t
ORIGIN      
        1 ggatttatta tcgctattta gatgaatttt ataggatttt tgctagtaaa ttaagttcga
       61 tcttgtcaga taagatcaat gtaaaaaaat cttttttcaa aaaagaaaca cctgttgaaa
      121 aaatcagtgt ggaattattg aaagaaatgc gtaatttgat gcaggaaggg atcatttttt
      181 cttttgatca agttggtttg atcgatggta aatatcgtat cccaatctat gtgattgaag
      241 attatatttg gaaattaaaa ggcacccggt atcgagaaga ggaaatcatc gctactttaa
      301 tctactctgt caccgatcga cactggcaat tggtagaaga tgccgatctt tcttatctgc
      361 cattgaagta gatctatttc ttatcaaaaa ataaaaagga aacaagaaac taggacatga
      421 ttctgtctca agtttcttgc ttctttttta tttacagctc ctgtcgttta tctggcacaa
      481 aaatcgaagg agtcgtttgt tggatcccta ataaaacgat gatcgttaca atagctgtac
      541 tcaacccgct ggcaccattc attacgaaag aaaagagcca aggattcatc ccccatagtg
      601 catacgctcc ccagaaaacc actcctgcaa taaaatgcca aaaatatctg gaaagtgcgc
      661 caatcaatgc accaaacacg atgctttttc taatttgttt cgtattatcg tctacatagc
      721 ctttttggac atttgaagcc atgagccctg ctgcgcctgc aaaagtatag gcaatcgggt
      781 actcaatcaa cacttgaacg acacttaaat aataaacttg tcccaacgga aaatgtagga
      841 tgccccaaat gaaacctgcc agcatccctg cctttgtccc tcttcttaac gcaaaaagag
      901 tcaatggaat ctgccctaat gaaatagaaa aactgctgcc gatctggatc ggaatcaacg
      961 acaacaccat cgctaacgct gcaacaatcg ttccttctac ccaaactcta ttttttccca
     1021 taaaaaaact tcctcctttg aataaaaaag tcacaaagga ggaagcagct ttcgaaaata
     1081 gtcaaacagc tgtttattca caattcctac gctcgcataa ccgatacagg tacgaagggt
     1141 ttagaaatag attcaatctc agcccaattg gactcccctt tgtgagttta tttattattt
     1201 attttttgtt tcaaacaatg gactaactgg tttgttttca tggatacgtt tgattgcatc
     1261 tcccattagt ccgccaacac ttacttcatc gattttgtcg attttacgat cttctggaag
     1321 atagatcgag tctgtaacaa ctagacgctc gatagctgaa tcttctattc tttgtaaagc
     1381 agggccggat aatactggat gtgtacatga agcatacaca ctagttgcac cagcttcttt
     1441 caaagcattg gcagccaatg tgatcgtacc tgctgtatcg atcatatcat cgatcagtac
     1501 acaagttttg cctttgactt gtccaatgat attcattact tctgcaacat ttgcttttgg
     1561 acggcgtttg tctatgatcg caatcggaga tttcaaaaat tccgctaatt tacgtgcccg
     1621 tgtgacacca ccatggtctg gtgaaacaac gacaacatca tcgcctttga ttcctttttc
     1681 taagaaataa ttggcaataa gtggcgcacc catcaaatga tcgactggaa tatcaaagaa
     1741 accttggatt tgaactgcat gaaggtccaa agtcagcatt cttgttgcac cagctttttg
     1801 aatcatgttt gctactaatt tcgctgtgat tggttcccgt gcacgtgctt tacggtcttg
     1861 acgcgcatag ccataataag gcatgactac gttgattgtt tttgcacttg ctcgtttcag
     1921 tgcgtcaatc atgatcagca actccatcaa gttatcattg actgggctgc tcgttgattg
     1981 aataacatag acatgcgatc cacgaatact ttcttcaatg tttacttgaa tttctccatc
     2041 actaaattga gtaacagatg attttcctaa ctcgacgccg actgctgctg caattttctc
     2101 tgctaatggg cgattggaat ttaacgcaaa aatttttaat cttgggtcaa agtaatgttt
     2161 tgacatgggg acctccactt tctttttcac aactctagaa ttctacctaa taaatagtag
     2221 tcatattttt taaataaatc aagtgatttt gcaaaagtct tagtttagat aagggagttt
     2281 ttttgcatat ccttctttat tgacttgtct tgctctcgca attgccagcg catattctgg
     2341 aatattgtct gtaattgttg acccggcagc aatcgaactg tttttagcta cctcgacagg
     2401 tccgatgata ttcgtgctag aaccaataaa gctgtggtcg ccgacagtcg tatgatgttt
     2461 gttctttcca tcatagttga cgaagacaac accacatccg acattgattt cttctcccag
     2521 cgtagcatca ccaacataag tcaagtgacc gactttagtt cctttgccga tttgagcatt
     2581 tttcacttcc acaaagtttc cgatatggac gttttcgcca atttcagctt ttggtcgtaa
     2641 atgtgcataa ggtcccacat ctgcatgttt cttgacatgg ctgctttcaa tgactgagtt
     2701 ttcaatgacg acgtgatcct caatcctact gtctacgatt tttgaatgtg acccgatgac
     2761 acaatcagaa ccaatgactg tatttccttg gatttgtaca cctgcttcga tcaatgtgtc
     2821 tggaccgatt tctacgcctg catcgatgta tgtcgttgct gaatcgacaa agcttacgcc
     2881 attgaccata tgcatctggt taattcgctt tctcatgatc tcatttgctt tagctaacgc
     2941 gatccgatca ttgactccca ttgattcttc aaaatcatcc gtttgataag cagcaactgt
     3001 atggccttct tcttttaaga tttcgatgat atctgttaga tagtattcac cttgagcatt
     3061 gtttgttcca actttgctta acgcatcgaa caaagcttcg ttgtcaaaac agtaagttcc
     3121 tgtattgatt tcttgtacaa gagcttcttc tggtgttgca tctttttgtt ccacgatttt
     3181 ttctacgatc ccaatatggt cacggatgat tcgtccatat cctgttgggt tttctgcttg
     3241 tgccgtcaag atcgttgcac ttgcattttt tccttggtga tattcaaaaa gattgttcag
     3301 cgtttcagta gtcaataatg gtgtatcgcc actgataact aacgttgttc ctttttttcc
     3361 ttccaagaaa gaagcagctt gcaaaacagc atgtcctgtt cccaattgtt ctgcttgtaa
     3421 cgcatattta ctgcgttctc ctagttgtgc tttaacttgt tctgctccgt gtccaacgat
     3481 cgtaatcact tcatctggtt tcgtttcact gacccgatta atgatatgtt ctaccatagg
     3541 tttaccagaa acgggatgta aaactttata taattttgac ttcatgcggg tacctttccc
     3601 tgccgccaaa ataattgcat aacgtgcgtc caactgcgtc actcctaaat atagtttgct
     3661 gatttcagtt tatcccagta tacccatttt ttcaacaaaa caagtgattt tcttttatcc
     3721 cagcgttttc tggataaaaa aatagataga ccataacggg cctatctatt tggtaaccgg
     3781 tgctctcctg ccaactcctc ggtcacaagt cacactactc ataaaacatg aaaagctgtt
     3841 ttttgaaatc gtttcttgtg acttagagtt aaacggtagg cttacaacct cttcattaaa
     3901 cgtttttcaa tcagctgacc ttcggtatta gctcaataaa cggcaaaata tgaggagcaa
     3961 tttttccgtt tattgagcta atactcagga caggacgctg atttcacaac ctctagataa
     4021 acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgaaaaaa tgtggaaagc
     4081 cattttcaca aaatttttct tactactcga aactaagcag ctttttcaca acctcttatt
     4141 ctgaaaaata atttcctggc acaaccgtaa tatttttcgt ttgagtatct acatctttga
     4201 cataaagcag tgacgtataa tcatcgatgg cgcgatgacc gttgaatttt gattcggcaa
     4261 atacagtgat gccaacaagt tctgattcga attcttcaat caagctcttc attccgttga
     4321 ctgttccgcc gcctttcatg aaatcatcca ctacaaggac acgtgaacca cgtttcaaac
     4381 ttctcttaga cagttccatc ttttcaattc tttcagaaga tccagacaca tagttgacac
     4441 ttactgttga tccttctgtt atttttgagt cccggcgtac aataacgaat ggcgcattca
     4501 gataatagga cacagcttgg gcaataggta cgccttttgt ggctacagtc attacagcat
     4561 cgattttttc accaaggtat ttagaagcaa tgattcttcc aacttgacgc agcagttcag
     4621 gttctcctaa caaatcagag agataaacat atcctcctgg aagcagacgg tcttgttctg
     4681 atagacgttc tgctagagat tcaatatatt ttttcgcctc ttcatatgaa atttcaggga
     4741 tgaacaatac accacctgca gcacccggga ttgtttccaa ggttccatac cctcgttctt
     4801 taaatgtttt cttgatgatt cccaaatctt cactgataga agatttagca gattcataac
     4861 gttcggcaaa gcttgttaag gaaatcaatt catgcgggtg attcaataaa tactgtgtca
     4921 tgtcaactag acgttcactg cgacgtactt tcacctattc caactccttt taagaaattt
     4981 tatcgtttta cattatataa gtttttcttc aaaaattcga gcattttatg aaaaaataag
     5041 aaaatagttc ggtatttatt aaaataaaaa gaagcctata aaaaattttt cgtttttata
     5101 aggcttcttt tctttataat ttagttattt tttcttctga aggcaaccaa tattcgtttg
     5161 atgatattta caatcaggaa caaagcaata aagactaatg tgattgttgc tcctggaggt
     5221 gtatctagat aaaacgagct agttagtcct gacagcatcc ctagaaatcc tacacaaaca
     5281 ctgatagcaa tcacgacatt gaagcttttg cctaatcgca taccgattgc tgcgggaaga
     5341 accatgattg cggatatcaa aagtgcaccg gcaatcggga tcatcacagc aatagccaca
     5401 ccagtaatca cattgaacat catcgacatc aaatgaacgg gtagcccatc cacgtgggcg
     5461 gtatcttcat caaaagtcag aacatacatc ggccgtttga ataaaaagaa caatataccg
     5521 atggcaacga ataagattcc taaaaagatg acttgctccc atgtaatggt cacgatcgaa
     5581 ccaaacagat aggattggat acttgttgct gaattccctc cgctcaaatt catcaatact
     5641 aacgctagcg ccaaaccacc agccattaaa atggcaatgg agatttctga ataggttcgg
     5701 tagatcgttc gcagatactc caatatcaca gctgctaata caacgactag catggtcgta
     5761 agtgtaggat taaggttcaa aaagaatcct aacgctaccc ctgctaaaga gacatgagaa
     5821 agcgtatcag ccatcagcga ttgtcttctg atcactaaaa acacacctag catcggggcg
     5881 attccggcaa tgaagatagc ggcaagaaac gctcttctca tgaattcata tgaaagcaac
     5941 gccattgaga atcctccttt ctcaccagtc ggatctgtct gtccgcatac tgtttgatat
     6001 cttcatgatc atgggtgatc attagaactg atttcccatg gacatgcgca ctatgtcgga
     6061 gaagacgata aaattcattt ctggattctt catccatacc tgttgttggt tcatcaagaa
     6121 caaataagtc agggtcagta gcaaaaatac gcgctaaact gattcgctgc ttttgtccgc
     6181 cagacaattc acctattcgc cgatttcgca tatcccacat ccctacagct tctaaagcac
     6241 gctgtacatg ttgatgatct ttttcagaga gacgcttgaa ccagcggcct cttggatatc
     6301 ttcctgaacg gactaattcg atcaccgtac taggaaaacc tgcattgaaa gatgcaactt
     6361 gttgaggaat ataaccgata cttaattttt ttccttcttg atttttctca gcaatcttta
     6421 tcattccttt tgaaggtttc agtaatccta acgatgcttt caccaaggtt gacttggctg
     6481 ctccattttc tccagttaaa atgacaaatt cacccgcatc tacatgataa gagacatctt
     6541 ctagtacagg ttcatcatca tagtaaaagg tcaaatcaga tatttcgata taacgcacct
     6601 tacaacctcc attctaaaac ttaattgata ctttctttta aagctttcag atttttttcc
     6661 atgacagaaa tataattttc gcctgctttg atttgttcat tagtcaagct ttctaaagga
     6721 ttcaacactt ctagcttcac tcctgtttca ttcgacaatg tctcagctac cttcgaagag
     6781 gcattttctt caaaataaat cactttgatg ttgtttttct tcacaaattc ttttagctca
     6841 gctaagcgac ttggtgtggg ttcctgatct ggtgaaacgc ccgaaatcgc tacttgtttt
     6901 aagtcgtatt gattcgctaa ataagcaaaa gcagcatgct gagtgacaaa tgtgcggttt
     6961 gttgcatctt ttagctcatc ggagtatttt tgatccagtg cttcaagctt tttgatataa
     7021 gcatcggcat tcttcgtaaa agtttccttt ttatctggat atttttcgcc taattcttca
     7081 gcaatcgttc tgacttcttt tatagctaat accggatcga gccaaacatg tggatcgtat
     7141 tcatgtgaat gaccatgccc ttcttctgat tcttcatgtt cgtctgcttc ttgcgattct
     7201 aaacggtcga ttcctttagc tgcttcgatc atcaaggtct gtttgctatc cactgaatcc
     7261 tttaacgaat ccacaaaaaa ttccatgtct gaactattat acacgaaaac atctgcatcg
     7321 gaaatttctg ctctttcttt tgcacttggt tcgaaatcat gcggctccgt tccagcaggg
     7381 atcaaaagtt tcacgtttcc ttcatcccct acgatctctt ttgtaaaatc atacatcgga
     7441 taaaaagtcg taacgatcgt caaatcctct tttttgtctg cttccttgtt cgtatttcca
     7501 catgctgcaa acagtaaagc acctatcatt accgtagctg tcaaaagata ttttttcatt
     7561 tatatgctcc tttcaatgaa caaatcggaa ttttacgatt tattaacgtg aactaatgta
     7621 tcaaaataaa aaaacacagt caatagaaat ccatcaattt ctgatttaag catgtatatt
     7681 cttgattaaa tcgtaaaatg gatgagttgc tgctcatatc tagccatttc actacaataa
     7741 aaaactgaag aataccattt taggattcct cagtttttca tcacctattt tacttcttat
     7801 attttattgc agggttcgta ctaaataaac ttcctcacaa aaacctttta acccattata
     7861 tactctctgc gctcttgaat aatgttgaca caaggcaaaa acggttggtc cgcttccact
     7921 catcaatgca gcatctgctc cgtacttgat catacgctct ttgatttgat ggatgactgg
     7981 atgccgcttt cccgttatat cttctaaact gtttcccatt tttttgatca tctgctgata
     8041 atcttgctct cggatggctt cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt
     8101 atctacttct ctaaacactg tacgtgtaga gacactcatt cttggtttga ccaacacaac
     8161 ccagcactgt ggcatagaag gtaatacttg gatttcttcc cctttgccac cgacaaatgc
     8221 cgtacttccg tagacacagt aaggaacatc tgtaccgact tccaaaccga tttctgccag
     8281 ttcttctaac gaacagccca gattccataa ttggttgatc cctcttagag cagctgcaca
     8341 gtctgtactt cctccgccta atccagctgc tactggaata ttcttcttaa caaagatctt
     8401 gacaccggtt ttgatattgt atttattttt tagcaataaa gcagcttgat aaacattgtt
     8461 ccgttcatct accggtaaaa atgctttatt tgtttctaca atgattttgt cttctgttat
     8521 ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac atcaccattt ccaactcatg
     8581 atacccatct tgccgtctat ataatacatc cagtcctaaa tttatcttgg ctggcgcttt
     8641 ttcaatcaat tccatcctct cacccattcg aaaagtctaa gtcgcaagta ttctatcata
     8701 gggaaaaata aaagtatagc ttatgtctgc ctagtggttg attttgtcat cgtttcaaca
     8761 gctttcacag aattttctct tcataatcca aacaagttct agtgattctt ataattctgt
     8821 gagcacttct tctgccaaac tcggaacatt tcctttttct tttcctatca gactgagtag
     8881 ctgcatggta gcaaatccga aattacttcc tttcctataa gcaaaataac gtccttccca
     8941 atgatcgaca tattttaatt tcttttcata agcagactgg aaggaataga aaaaattacc
     9001 ataattatat acgatatgca tgactttttc ttgaaagaaa gaaaaatctg tttctccaac
     9061 attagctaat ggagctaagc ctagattggc ttccgagtag cccattttct cgtatacaat
     9121 aaacagattg gctaacagat agtcagctaa ttcctcagat tctttaggta agacccgcaa
     9181 caaatcatat cccgcctgct tcttcgtcat cggctgagcg gtgatgaagc caacgacttt
     9241 ttgatcttta cggacaatac cgacaccgct agataataca tatgatttat caaatcgtcc
     9301 cacagagaag tatttttctc gtgcaccttg aagccatttt tgtgaaactt catttacagc
     9361 ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa
     9421 acgttgatat tcaagttgct gactcagccg agacaagtct tctgttgcta gatcaacaat
     9481 ccctgcttca cctactttcg tgaagtgaaa acccaattca tgaagcatca ctacgtagct
     9541 ttctgatatc ttataaaacg caagctgata gcctaaacaa tctgcttgtt tcataaactg
     9601 attcgttgct gcccgcatca ttttaggatt gccaactgga tctcctaaaa caaagcattt
     9661 gtttcctttg atctgataac caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa
     9721 ataactgtaa cctgacaagc gaagctgatg gcttgtagct gtgcctccgt atgtttccaa
     9781 taaacggtca aaacggtctt tttcgtactc actgccaagc ttcgtagtat cttcagctaa
     9841 atattgataa agcgccacta aagctagcaa tgagatgccc aaaccaataa gccctgagaa
     9901 ccacacatca tctgaaggaa acaaaataaa atgatttcct gttattcgac cgactaaaaa
     9961 gcgtccgctg tgatagcctg caattgcgta aacgataaac aaaaagccga acaagctagc
    10021 atcaaagatt atagctcccc aagaataaac gaattttgtt cgataaaatt cttttctgga
    10081 taaccacaca gctaacagaa tcaagacata taccaatatc aactgccaag ataacgtacg
    10141 tgtgattgta tttaaaatac caaaagatag taaagcaatc gtaggcagaa atgccttttt
    10201 gacttttact gaaagtgctc tagcaaggcc gagtaataaa aagccaacca atagattcac
    10261 tgtctgatct aaaaaattga atgagaacgg caataatact tgaaatagcc gactgattgc
    10321 agacagatta gtgattgttg atagaagtac catcatgatc cccgcaaaat agacagcact
    10381 tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg
    10441 attgattttg actcctgtat gtgtgatgaa caatagaagt cccgtcataa aaggtagaac
    10501 ataataaaac aaccgataat ataatagcca aactactgct acatcttgcc gaacaggcaa
    10561 ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt
    10621 cagcattcct atcaatgtag caatcatgaa cataggatag atcgaaagca tcgaaatatc
    10681 tattctcatc aacttgccga tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa
    10741 ggatgccagt agcagaccaa gcaagccttt tggataaaac tcgaagaaca atgtctttct
    10801 ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa ctaccgccga aaagccagat
    10861 ccaatagctt cgaaaaatac tttctgactg aataaaaaag acatcaataa atgtaagaaa
    10921 cgaccataca gacaaaccag tcagcataaa tagtgcgact tttgatacag tggctaaaac
    10981 catttttcta tttgtctgct ttccataaaa actagcccgc agacttgctc ctaccacacc
    11041 accaaaaccg gcgagattgt tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt
    11101 tctttccatt tttggttttc cttgtttttc taagactctg atcgtcaccc aatcatacaa
    11161 caacatcgga gtgacaccca gcagtccagc cagaatcatg agcaggatcg tcatgcggct
    11221 ttgttgtccc atcctttgcc aaatatcttg ccaactcatg ccattagcaa tatttgcgac
    11281 ttgatttgcc acaaagacta acacggagcc gaaaaaaatc agttttaaaa tcagactatg
    11341 ttcctttatc cattgcagca tcaagtatcg tttatttttc acattttccc attcctaact
    11401 tgtatacttt tgtgtcatac cccaatgtta tcttatttat tttaattttt ccactttaga
    11461 cctgttcttt gcttgcgatg atagaaaaag aaagatatcc taatgctaaa ggagaggata
    11521 acatgaaagt aggatttatc ggcacaggga atatggcaca agctattatt gatggtttat
    11581 taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc cagtggacat tttgaaaatg
    11641 cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag gaagttgcat
    11701 cacaatcaga tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa gtattggaag
    11761 aactaagaga atatagccag aaagtcttat tcatttctat tgcttcagga aaatcattaa
    11821 cagatttaga aacagcgctt gaagatcctc aggcctccat cgtacgtgtg atgccgaatg
    11881 tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct gccacggagg
    11941 aacagcttca gcaagctacg atgatttttg aagcaatcgg cagcgtctat cctttagcag
    12001 aatcagattt cagtacattc attggccttg ctggcagctc acccgcattc gtttacatgc
    12061 tgatcgatgc gatgggacgg actggtgtac tgaatggttt gtccaaacag acagccactg
    12121 agattgctgc aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt gataaaaatc
    12181 catgggaact tgtagaccaa gtttcttctc ctggcggtac aactgttgca ggtgtagttg
    12241 ccttagaaga agcaggattc atcccagcaa tcatcaaagg aatcaatacg acgatcgaga
    12301 aagatcttga gatgttgaat aaaaaataa
//
LOCUS       DAZKCZ010000075        12113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000075 DAZKCZ010000000
VERSION     DAZKCZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.076"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(5..562)
                     /gene="frr"
                     /locus_tag="ACG8KN_001894"
     CDS             complement(5..562)
                     /gene="frr"
                     /locus_tag="ACG8KN_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9188942.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(565..1287)
                     /gene="pyrH"
                     /locus_tag="ACG8KN_001895"
     CDS             complement(565..1287)
                     /gene="pyrH"
                     /locus_tag="ACG8KN_001895"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9188943.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1423..2304)
                     /gene="tsf"
                     /locus_tag="ACG8KN_001896"
     CDS             complement(1423..2304)
                     /gene="tsf"
                     /locus_tag="ACG8KN_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9188944.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2403..3185)
                     /gene="rpsB"
                     /locus_tag="ACG8KN_001897"
     CDS             complement(2403..3185)
                     /gene="rpsB"
                     /locus_tag="ACG8KN_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9188945.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3544..4023
                     /locus_tag="ACG8KN_001898"
     CDS             3544..4023
                     /locus_tag="ACG8KN_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9188946.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4240..5331
                     /gene="phnW"
                     /locus_tag="ACG8KN_001899"
     CDS             4240..5331
                     /gene="phnW"
                     /locus_tag="ACG8KN_001899"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFP9188947.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5324..5452
                     /locus_tag="ACG8KN_001900"
     CDS             5324..5452
                     /locus_tag="ACG8KN_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFP9188948.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5456..6001
                     /locus_tag="ACG8KN_001901"
     CDS             5456..6001
                     /locus_tag="ACG8KN_001901"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFP9188949.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6455..8146)
                     /gene="argS"
                     /locus_tag="ACG8KN_001902"
     CDS             complement(6455..8146)
                     /gene="argS"
                     /locus_tag="ACG8KN_001902"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9188950.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8566..9513)
                     /gene="arcC"
                     /locus_tag="ACG8KN_001903"
     CDS             complement(8566..9513)
                     /gene="arcC"
                     /locus_tag="ACG8KN_001903"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9188951.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9628..10647)
                     /gene="argF"
                     /locus_tag="ACG8KN_001904"
     CDS             complement(9628..10647)
                     /gene="argF"
                     /locus_tag="ACG8KN_001904"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9188952.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10738..11967)
                     /gene="arcA"
                     /locus_tag="ACG8KN_001905"
     CDS             complement(10738..11967)
                     /gene="arcA"
                     /locus_tag="ACG8KN_001905"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFP9188953.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3309 a         2518 c         2193 g         4093 t
ORIGIN      
        1 tttattaaac ttccaataat tctttttctt tatcagcagc gatcttatct acttctttga
       61 tgctgttatc tgtcaatgtt tgtacgtctt tttctaatcc gcgcaaatca tcttcggtaa
      121 tatcaccatt tttttgttgt tttttatatt catcgatcgc atcacgacgg atgttacgaa
      181 tcgcgatttt tgcattttct gcttcttttt tcacatcttt agctaattct tttctgcgtt
      241 cttctgtcag ttgcgggata actaagcgaa tgacatttcc atcattggtt ggactgattc
      301 cgatatcact tgccataata gctttttcaa catcttgaag aatgctcttg tcaaaaggtg
      361 tgatcattaa aacacgtgct tctggaattt ggattgatgc catttggttc aatggtgttg
      421 gcgcaccgta gtagttcaca gtaatacggt ccaaaaggct agcgttcgca cggcctgcgc
      481 ggatttgccc aagttcacgt tgaagatttt gtgctgcttt tttcattttt tcttttgctt
      541 ctgtcataat tgcatctgcc atttttattt cccccttaca gttgttccga tattttcgcc
      601 taagattgca cggcgaatat ttccgtgttc atttaaattg aagacaagca gtggaatatc
      661 attatccatg cttagtgaac ttgcagttga atccatgact tgtaatcctt tggcaataac
      721 ttccagatga gtcagttctt caaattttac tgcatttgca tctactttcg gatcagcaga
      781 ataaacaccg tctacattgt ttttagccat caggataaca tctgcgccga tttctgccgc
      841 gcgtaatgct gctgttgtgt ctgtcgagaa gtacgggttg ccggtaccgc ctgcaaagat
      901 aacgatccgt cctttttcta aatgacgttc tgctttacgg cgaatatatg gctctgcgat
      961 ttgacgcatt tcaatcgaag tttgcacgcg tgtcggtaca cccacgtttt ccaacgtatc
     1021 ctgtaaagct aaggcattca taactgtagc taacataccc atgtaatcag cttgagcacg
     1081 ttccatgccc atttgtgctc cgatttgtcc gcgccagata tttccgccgc ccacaacgat
     1141 tgccatttca acgcccagct cgtgaacttc tttgatttct tggatgattt ctttgattac
     1201 tggtggtttg attccaaatc ctgcgtcccc agctaaagct tctccactca attttaagac
     1261 tacacgctga tatttcggtg tgaccatgtt cattcctcca atattttcta ttgccatttt
     1321 accatactta gatgatttcg acactctaag actggttttt cttgttttgc ttaaaaaaag
     1381 ggagcgcaca aaccgtgcgt tcccgtctgt tattcaacag aattattttt tcacttggct
     1441 cataacttct tctacaaagt tatcttcgcg tttttcgata ccttcgccaa cttcgaaacg
     1501 tacgaatgtt ttaacagttg cgccttttga tgcaacgtat ttttcaacag tcatatcagg
     1561 atctttaacg aatggttggt ccaccaaagc gatttctgct ttgaatttct tcaagcgtcc
     1621 ttctaccatt ttttcaacga tattcgctgg tttgccttcg ttcaatgctt gttctgtaag
     1681 aacagttttt tcatgttcta attcagcttc tggaatttgt gattcgttta cataacgagg
     1741 attgatagct gctacgtgca tagctacgtc tcttgcaact gtttcgtcag ttgttccatc
     1801 tagaacagtc aatacggcaa tacgtccgcc catatgcaag tatccaccaa atgcagcatt
     1861 gtcgtctttt tctacaactt caaagcgacg gaagctgatt ttttctccga taacttgagt
     1921 tgcttcgatc aagtcagatt caattgtacc tttatctgtt ttgattttca ttgcagcttc
     1981 catgtctgct ggtttgtttt cagcaacaag ttctgcaatt tcttttacta aatcttggaa
     2041 catttcgttt ttagaaacaa agtctgtttc tgagtttact tcaacgattg ctgcagtgtt
     2101 acctttcaca gcaactgaag ccaatccttc agctgcaatg cggtcatttt tcttcgcagc
     2161 ctttgccatt cctttttcac gtaaaagatc aactgctttt tccatgtcgc cttctacttc
     2221 aactaatgct tttttcgcat ccatcattcc tacgccagtc atttcgcgta gttcttttac
     2281 cattttagct gtaacgtctg ccatgtttaa ctcctcctgt gtgtttttct ttttaaaaaa
     2341 agctgcttca aaggagagtg aggcttgtcg cccagccttt gaaacagctc catcattcaa
     2401 aattattctg ctgaagaatt gtcgccttca acaacatcta cgatttcttc gattgatgtt
     2461 gctgtgtttt cttctgtgaa atcttcttca actacttgat cttcaccttg gtttccttcg
     2521 atgaaagcat cagccatttt tgaagtaatc aatttaacgg cacgaattgc atcgtcgttt
     2581 gaagggatta ctacgtcgat ttcatctgga tcgcagtttg tatcaaccat agctacgatt
     2641 gggatattta atttgtgtgc ttcttgaaca gcaatacgtt ctttacgtgg gtcaacgatg
     2701 tacattacat ctgggattct tggcatatca gcgataccgc ctaagaattt ttcaagacgt
     2761 tcacgttctt tgttcaaacc agcaacttct tttttaggta gaacttcaaa agttccatct
     2821 tcttccattg cattgatttg tttcaaacgg ctgatacgtt tttggatagt atcccagttt
     2881 gtcaatgttc cgcctaacca acgatggtta acgaagtatt gtcctgcacg agttgcttct
     2941 tctttgattg cttcttgtgc ttgttttttt gtacctacga ataatgctac gccgccttct
     3001 tcagcaacgc tcttcatgta atcataagct gcatctacta atttaactgt tttttgcaag
     3061 tcaatgatgt agattccgtt tctttctgtg aagatatatt tcttcatttt tggattccag
     3121 cgacgagttt ggtgaccaaa gtgtacgccg gcttctagta attgtttcat agaaattact
     3181 gccatgtttg tttcctccga tttggtttta tttttcctct tcttatcttc taccttgccg
     3241 gcaaactttt tcaagcacct accgacaatc caataagaat gtggatttgc tgatgcataa
     3301 agcaccctca gctattatac agacaaaaga tccgtatcgc aagagcaaat atacttttct
     3361 gctcaacatt caatagttta cctgaaagat aaaaagaatt caagaaaaac agtagatttc
     3421 tctttgtttc tttattatcc tttttccata aaataatttt aggtataaaa gacgaatatt
     3481 attgggcaat ccttcttttt tttgttatga tacatgtgaa atctttcgta aaggagaaag
     3541 aaaatgcgca aagctcaaag acatttgctg attaaacaag tcattgaaga attcccgatc
     3601 cgtacgcaag aagaactatt aaacaaactt gggcattatg gcgttaccgc cacgcaggca
     3661 acgatttctc gagatatccg tgatctaaaa atcgtgaagg cacctgatga aaatggcgtt
     3721 tcccgcttcg tattgtttcg cgaaaaagga actcctgaga ctaggactga ggaagaggat
     3781 cggctggttc aaatgattga agacattgta ttgaaagtcg aacgagtcca ctttttgacg
     3841 atcgtcaata ccttgccaga caatgctcat ttatttgcat ctgttttaga tgagatcaaa
     3901 cctccctcta ttgtcagtac aattgctgga ttcgatacga tcatggtcat ctcggaaaat
     3961 gaggaggacg caaaacgagt agaagaattt cttcaccatc caactgaagt atcacttttt
     4021 taatcagagc acgtattcct aatatggaat gcgtgttttt ttattttcaa tattttcctt
     4081 attgaataaa cctttacact ctttacaaaa atacatcatt ttttattcaa atttttcaac
     4141 accttgattg aatcacaata atcatcattt atattggatg ttaacaagag cagcttcttg
     4201 ctctttgtgt atttcaaaaa agaaatgaaa ggaaatcata tgtataaatt attaacttct
     4261 ggaccattga cgacttctga aacagtgaaa aatgctatgc tgatcgatca ttgtacttgg
     4321 gatcaagaat acaaagaaat tacacaatgg ataagaaaaa aattattgga tcttgctcaa
     4381 gtatcggaag gaatatacac aacggtctta atgcaaggta gcagctcctt cggggtagaa
     4441 tcggtattaa gttctgtgat caggccagaa gatactgtac tgatttgttc aaacggcgca
     4501 tatagacaac gaatgatcaa catgtgcgaa tgtctgaaac tgaactatac cgtttatgag
     4561 gtatcggaac atcaaatcct ccaagctcat gtgattacac agctgattga agagaatccg
     4621 tctatctccg cactagcaat ggtccatagc gagactactt ccggtatttt gaataatttg
     4681 gaagagattg ctgctgtcac acaagaaaga gatcttttgt ttatagtgga cgcgatgtca
     4741 agctttggtg ggataccgat ttgtgtagcc gaactaggga ttgatttttt gatttccagc
     4801 tcaaacaaat gtattcaagg tgttccaggc ttttcttttg tacttgctaa taaagcggaa
     4861 ctggaaaaac gaaaaaatgt tagccgaagt ttgtcacttg atctatatga ccagtaccgc
     4921 gtgatggaaa acgagaatgg caaatggcgt ttcacttccc ctacccatac ggttttggct
     4981 tttcaacaag cgctgaagga actagaggca gaaggaggaa tcgaaactcg atattcacgt
     5041 tatctcgcca ataatcatgt gctacgacaa aaaatgacag agatggggtt ccagcctttc
     5101 attcaagaat atcaggggcc attcatcacc tcttttcttt accctaagga ctggcgattt
     5161 gattttaatc atttttatca gtatttaaag caaaatggat ttgtgatcta tcccgggaaa
     5221 atcagcgatc atgaatgttt tcgtatcgga aatattggcg agatctatcc ggaagatatt
     5281 ttggaactga cagcaattat caaagactat aaggagaacg ttaatgatta aaactgtaat
     5341 ttttgattgg gcaggaacaa ctgtggactt tggctgtatg gctcctgtcc acgcttttag
     5401 aaatgctttt ctagaaaaag gaatccaact gactgacaaa gaaatccgct aaccgatggg
     5461 aaaattaaaa tgggatcata tccaacagct tctttccctc ccttctgtca aaaaacaatg
     5521 gatagctatt tatggacaac tacctcagga aaaagaggtg gaggaattgt atcaacaatt
     5581 tgaacattat ctgtttaaag atttggtcaa gcattctacc ttgaaacccg acacattgga
     5641 aacgatcgaa cggttgagaa acgctgggat caatattggt tccacgaatg gatttaatgc
     5701 tcctatgatg acaatcgtag cagaaaccgc aaaaaaagaa aggtattcac cagacttcat
     5761 ggcaactttt gaagacgtcg aagggtatgg tcgaccgttt ccttatatga tccataaaaa
     5821 catgcagtac ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag atacggtctc
     5881 agatatccag gaagggaaaa atgccggagt gttgacagta ggtgtaattg aaggtagttc
     5941 gctaatgggg atttcttatg atgagtacca aaaattagag atggggaaaa agatcgttta
     6001 aaacagtctg ttgctcttag attccaacaa gccggcgctg atatttgtgt aaacaattta
     6061 aaagagttaa cagaatacat tttgtacgct gacaagaatc cagaatgact tttatacaaa
     6121 ataagaggtt gtgaaaaagc tgtcctgtat caagtaataa aaacagccaa gcaaaaatag
     6181 ctcctcatat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt
     6241 ttattcgaaa aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc ttatttgcaa
     6301 acaaacagtg tttaagagac aacctctcca ctaaacggtg ttcattcagc tgactctcgg
     6361 tatttgctca aaaaacgata aaatttgaaa agctattttc tcttttgttt ctctaatact
     6421 cggatcagaa cgctgatttc acattctctt tttcttacat cttatctggt gcatgaagtc
     6481 ctagcaaacg aaggtcttct ttcaataata cagtcactgc ataaactaga gctaagcgtg
     6541 cttctttttg ttcatcatca gctaaaatct tcgtatgagc atagtatttg ttgaatgctt
     6601 gtgctaattt gattgcatgt ttcgcaatca ctgacggttc gtatttttcc ccagcagaca
     6661 agaccgtttc agggtatttt tgaactaatt taaccacttc ccagctggta tcgtcattca
     6721 atgcatagtc tgctgtttct gaagggacaa atcctgcttt ttccaacagg ctgactgcac
     6781 gagcatgtgt atattgcaca taaggtccag tttctccttc aaatcgaaca acttcttcca
     6841 ggttaaagtc aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg actgccccaa
     6901 ccccaacttg tttagctaca gcatctttgt tttccaaatc agggtttttc tcgctgattt
     6961 gttctttagc agattggatt gcttcattta atacttcttc taataagacg atctttcctt
     7021 tacgagttga taatttttta ccaccttgcg tgatcaaacc aaatggaata tggtgcatgt
     7081 cgtcagacca gtcaaagccc atttccttca agacagcttt caactgttta aaatgatagc
     7141 tttgttcatt ccctactaca tatagagatt gtttgaagtc gtaagtacgt ttacggtaca
     7201 atgcagcagc taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc ttaatcaacg
     7261 ctggattcag gtcataagca gataggtcaa cgatctctgc acctttatcc tcgttcagca
     7321 aatgtttttc ttctaaaagt ttgacgattt catccatctt gtcattgtag aaagcttcac
     7381 cgttcaacga atcgaaacgt acttccagca gatcatagat tttattgaat tctttcatcg
     7441 attcatcacg gaaccattgc cataattgaa tagcctcttt atcgccttct tccagacgtt
     7501 tgaaccaagc acgagcttct tcatttaatt ctggttctgt ttcagctact tcatggaatt
     7561 ggacgtataa acgaagcaat tcattgattg gttctgcttt aacagcttct tctgtacccc
     7621 attttttgta agcgacgatc agttttccaa actgtgttcc ccagtcacct aaatggttga
     7681 tgcggattgg ctgataaccg attttttcca taatgaaacc gatcgaatta ccgataactg
     7741 tcgaacgcaa atgtcccatt gagattggtt tggcaatatt aggagaagac atgtcgattg
     7801 gtactgttcc ttgattcccg atgttgctat ccccataatg ttctttttct ttcactacgg
     7861 tttgcaaaac ttttttgctg ataagttctt tattcatgaa aaagttcaag taaggtccga
     7921 caacttcgat tttttcaaag ttggcagaat cgattttttc tgctaaatct gcagcgattt
     7981 gctgtggcgc ttttcggtaa acttttgcta atgaaaaggc tggaaatgca acatctccgt
     8041 gttcagctga ttttggattt tctagtaatt gctcgacttg ttccagtgtt aaatcatctt
     8101 tgacagcgtc atagacagct tttgcgacaa gatctttatt attcatcttt attcctccta
     8161 aagtgttccc ttgattgtta aacaaaaaaa atctctagcg acggcatact tagtatgcca
     8221 tcactagaga cgagatttcc cgcggtacca ctccaattgt cctaaaaaag gaccagctcc
     8281 atctgatatc ggtagacgac cgtctgttta aaacagaatt ctccaagtgc gcttcgttgt
     8341 atcttcttca ttcggcttcc accacttccg aattcgctag ccaagaatga tacaagtact
     8401 ctcttgttca acaaatctta gtcttattaa actttcttga attatatcag aaaaaaaaac
     8461 gaaaggctag tcgtttcata aaaaaaaaat ccagatggcc taaaggagaa gaaataccac
     8521 ctggatagct ataaatcatt tcgtaaatac gaaagactta acaaattaat cagctacgat
     8581 aatcgttcct tcttcagaag ctaaaagatt ctcgatattt tctaaagaag tgatgattgc
     8641 cttagcgttt ggttttgctt caacaaatgc aatagctgct tctactttcg gaagcatgct
     8701 tcctggagcg aattgctttt catcaatgta ttgtttcatt tctgaaacag taacagtttc
     8761 taatttcttt tgatctggtt tttggtaatt gacatacaca ttgtctactc ctgtcaatac
     8821 gatcagcaaa tccgcatcga tgatttcggc taatttttct gaagcaaaat ctttatcgat
     8881 tacggcttct cgtccttcta atccagtagc tgtttctaca acaggaatcc caccgccacc
     8941 aacagagact gtgataactc cttgatcgac cagcgtttcg atgacacgtg cttctttaat
     9001 cgagatgggt ttaggggaag cgactacttt tctccatcca cgtccagcat cttctttgaa
     9061 tgtcacagtg gagtcagcat tcatttgttc tttggcttct tcttccgtgt agaatggacc
     9121 gactggttta cttggatttt tgaatgaagg atcattttca tccacaataa cttgagtgac
     9181 taaagaaacc acatctttat cgatcccttt ttccttcagt acttcgccca tggcgttttg
     9241 taaccagtaa ccgatagatc cttctgtcat tgcaacacat gtatctaaag gcattgccgg
     9301 tgttttctca gaatcagcag cttgctgctg aatcaatagg ttaccgactt gcggaccatt
     9361 tccgtgggag atgatcaatt catctccttg ttcgataaat ttcactaaat actttgctgt
     9421 ttccattaac gcttcttgtt gggcttttgc acttgcatcc gtagacaaga tggcattgcc
     9481 ccctaaagcg acaactactt ttcgtttggc catttcattc gtcctttctt cagttctacc
     9541 gaattttcta gataacaaaa cgagccagcg ttttgtgatt ttccctcaat gctgactcgt
     9601 tttttcgtga gatgaatgtg gttatgctta aacgcgaggg ataaacaagt tacctaatgt
     9661 tgctgccatg atcgctttga ttgagtgcat acgattttct gcttggtcaa attgacgagc
     9721 atatttgctg cggaagactt catctgttac ttccatttca gtgatgccaa agcgttcttt
     9781 catttgttca ccgtaaacag tgtttgtatc atggaaagct ggcaagcagt gcaagaagat
     9841 caaacgatct gtgttgtgtg ttttttctac catttccata ttgatttggt aaggtttcaa
     9901 caatttgata cgttcttcga atttatcttc ttcacccatt gatacccata catcagaata
     9961 aaggacgtct gctccgtcta cacctttatc aacatcgtcg gtaatcatca attgtgcacc
    10021 ggatttttca gcaaagcctt ctgccatttt gacgatttct tcttcaggtt gtaattcttt
    10081 tggtgcaacg atacgcatat tcgctcctaa aattgcacct gtgactaata aagagttagc
    10141 catattgttt cgaccatctc cgcagtaagc aacagtgata ccttctactg ttccaaagtt
    10201 ttcttggata gttaaaaagt cagcgatcat ttgtgttgga tgccattcat cagtcaagcc
    10261 attccaaact ggaacaccag agaattctgc tagttcctct accatttttt gactgaatcc
    10321 acggaattcg attccgtcaa acatacgtcc tagtacttta gctgtatctt ctgtggattc
    10381 ttttttacct aattggatat catttgcacc tagatattca ggatgtgcac ctagatcgat
    10441 cgctgcagta gtaaatgcag aacgagtacg tgtagatgtt ttttcaaata aaagcgcaat
    10501 attttttcct tctagatagt gatgtgggat accacgtttt ttcaagtctt tcaagtgttc
    10561 tgaaaaatcg atcaaatatt gtaattcttc ttttgtaaag tctttttctg ctaataagct
    10621 tcttccttgg aatactgatt ctttcatatt aaacattctc ctttagattg tttttattta
    10681 agattaaaga ggaaatcccg acggcagctg acagtaagac tgccgtcagg aaacaaatta
    10741 cttcaagtct tcacggacaa gtggttggct catgcagcgt ggaccaccac gacctcgtga
    10801 caattcactt gaattgattt ctaacacttt gatgccatag ctacgaagca attcgttcga
    10861 tacataattt cggttataag tgactacaac acctggagcg attgccaatg tgtttgatcc
    10921 atcattccat tgttctcgag gtgcaacgat ctcatctcca ttaccagttg ggatcagtac
    10981 aagatcatct aatccaagag cagctttcat cgtttcgtgt aagttatctg aatgagtaat
    11041 cttgatatca tcaccatctg gttcgattgt aaagacatcc atcttaccgt ctttactttg
    11101 aattgctgga tggatagtga atttatcgtg atctaccatt gtaaatacgg tatccaaatg
    11161 catcattgca cggttgttcg gaattttgat tgccaacact ttttcaaatc ctgaattttt
    11221 tgcgaatagg tggcgtgcta atttttctaa agctttcgca ttagttcgtt gagaaatccc
    11281 aacagctact actttatcgc ttaagatcaa ttcgtctccg ccttcgatat gatctggatt
    11341 ttcacgatcc aaccatactt cgattccttt atttgcaaat cttggatgat gtttcaaaat
    11401 atattctgta aacatcgatt cacgacgacg tgcttcaaaa gtcatcttat tgactgtcat
    11461 cccatttccc attgatgcgg aagggtcgcg tgtaaagtac aagtttggca ttgggtccat
    11521 gtaaaatgga tagtcatcgt ctgctgacag atcatataga ctgctagaac gtacttcgat
    11581 atcttttgta cgaaccccag ccatgatttt atctaccatt tcttgcgttt ccattgatag
    11641 aaggaactca tgaagaccct ctcttacagc attcgatgca atatgagatt catccaacat
    11701 tttattcaag aattgttctt tgacattgcc tgcgtcaatc gcttctgccg ctaatttttc
    11761 caagtaaaga gtctcgacgc cttcattttg caatgtcttc gcaaagttgt catgttcttc
    11821 ttgagcaatc ggaagatatg gaatatcatc gaacaacaga cgatccataa tatctggtgt
    11881 caaattttcc acttcttgtc caggccgttt cagtaaaact gtctttaatt tccctatttc
    11941 agagaaaacg tgaataggtt tatccatgtt gtgttgcctc cttctttttg attacatgta
    12001 taggatatcc tccttttgta atcactttca taattttgtc tcattcaaaa tgagacattc
    12061 ctcacattaa tattcgataa ttaaataatt aaacgttttt ctatttttaa gcg
//
LOCUS       DAZKCZ010000076        11835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000076 DAZKCZ010000000
VERSION     DAZKCZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.077"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(277..1125)
                     /locus_tag="ACG8KN_001906"
     CDS             complement(277..1125)
                     /locus_tag="ACG8KN_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9188954.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1289..2191
                     /gene="gnd"
                     /locus_tag="ACG8KN_001907"
     CDS             1289..2191
                     /gene="gnd"
                     /locus_tag="ACG8KN_001907"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFP9188955.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2193..3194
                     /locus_tag="ACG8KN_001908"
     CDS             2193..3194
                     /locus_tag="ACG8KN_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFP9188956.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3191..3475
                     /locus_tag="ACG8KN_001909"
     CDS             3191..3475
                     /locus_tag="ACG8KN_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9188957.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3508..4305
                     /locus_tag="ACG8KN_001910"
     CDS             3508..4305
                     /locus_tag="ACG8KN_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9188958.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4319..5152
                     /locus_tag="ACG8KN_001911"
     CDS             4319..5152
                     /locus_tag="ACG8KN_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9188959.1"
                     /translation="MEILSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5170..6027
                     /locus_tag="ACG8KN_001912"
     CDS             5170..6027
                     /locus_tag="ACG8KN_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9188960.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6049..6558
                     /locus_tag="ACG8KN_001913"
     CDS             6049..6558
                     /locus_tag="ACG8KN_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9188961.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6595..6996
                     /locus_tag="ACG8KN_001914"
     CDS             6595..6996
                     /locus_tag="ACG8KN_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188962.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7108..7461
                     /locus_tag="ACG8KN_001915"
     CDS             7108..7461
                     /locus_tag="ACG8KN_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188963.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7495..7938
                     /locus_tag="ACG8KN_001916"
     CDS             7495..7938
                     /locus_tag="ACG8KN_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9188964.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7955..9040
                     /gene="uxuA"
                     /locus_tag="ACG8KN_001917"
     CDS             7955..9040
                     /gene="uxuA"
                     /locus_tag="ACG8KN_001917"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9188965.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9033..9746
                     /locus_tag="ACG8KN_001918"
     CDS             9033..9746
                     /locus_tag="ACG8KN_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9188966.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10075..11376
                     /locus_tag="ACG8KN_001919"
     CDS             10075..11376
                     /locus_tag="ACG8KN_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9188967.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(11454..11603)
                     /locus_tag="ACG8KN_001920"
     CDS             complement(11454..11603)
                     /locus_tag="ACG8KN_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188968.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         3943 a         1939 c         2515 g         3438 t
ORIGIN      
        1 ccaaaatttt ttttaatttt ttagaaaata agaaacaaaa ataataaaaa aaagaaaaga
       61 taaatttttt actactgtat actaactttg ttatcgcatc taacaagtaa ctcgaataat
      121 accaatacaa ccttctccaa agatgcgatg acatacgttc taacccaccg actattgatg
      181 ttctgtacca cccttgcaga accaaaaaag ccactaccaa atatttggta gtggcttttt
      241 ttgttttatg gatttttcgc tttttctgtt atggtgctat tctttgattt cagaaatagt
      301 atgtcgaact ttagcgatag aacgacgtga ttctttttcg ttgtaaaaca tcaaaatcac
      361 atatagagag tccaaaatgc ttagttgaga aatacgggaa gctaaagctt cagatcggaa
      421 ttctgtttct tcagaaatag aaataaaaac gacatcgcca agtttagcaa gggaagaatt
      481 agcttggcta gtaataacga ttgtttttgc tccagttttt cttacggttt cagcaatatg
      541 gattgtttct tttgattgcc ctgtgtgaga aatcaaaaca gcacagtctt tttccgtcaa
      601 taaagaagct tccatcaatt gaatatggta atccgtgctg tgagaagttg cgatagggga
      661 acgcaaaaat ttatgataag cgtcagttgc tacaatctct gaaccgccaa caccgaaaaa
      721 atagacacgt ttagattcat tgataatagt agccgctttt tttagatcct tttgttgtaa
      781 caactttttt gtgtcattta aagttgaaat actagactcg aacacctttt gcgccatagt
      841 gagctcagta tctgttttct gaatattttc gtggatagaa atagcagata gatcattttc
      901 ttggatcaac atggccattt taaaatcttt aaatccatta taaccaagtt tttttgtaaa
      961 ttgaaaaaaa gtagagtcag caatgcccag ttcacttgct aaattgctaa taggactatg
     1021 acttactttg cttggatttt ctaaaatata atcggcgata gcctgttctt ttgggctgaa
     1081 gtcgttgtac attgttttga tttgtagtct tatggagacg ctcatgagaa ttcctcattt
     1141 tcataataag attatcattt tttccgttct cattaatgtt aaagatacaa ggagagtagg
     1201 aaaaaatcaa acaaaaaata tttttgtaaa atctatttac aaaaaaattt ttttgtataa
     1261 tgagggtata agaaatggag gcgctatcat ggaaattggt gtaattggtt tgggaaaaat
     1321 gggattgaat ttagctttaa acttaaaaga gcaccatcac aaagtacaag gactcgacat
     1381 ttcaagtaca gctgttagtc aggcaagaga aaatgacatt cctgcttatc aagagatctc
     1441 tgaatttcta gctacttttg agcagaaaaa aatcatctgg ttaatgcttc ctgctggaga
     1501 gataaccgaa aacatgttaa gaaaactttt tccaaaactc ggaccaggag acattattat
     1561 tgaaggcgga aactcttatt ataaagattc aatccgtcga gcagaagagt ttcagaaaaa
     1621 tgaaatcggt tacttggatt gtgggacttc tggaggaatc gaaggagcac gacatcaagc
     1681 ctgcttaatg attggaggag ataaagaaac ttttctatcc gtggaacaat tattcaaaga
     1741 tgtagcaatt gaaagaggat atctgtatgc tggtgcatcc ggaagcggac acttcttgaa
     1801 aatgatccat aatggaatcg aatatggaat gatgcaagca atcggtgaag gattccaact
     1861 tgtacaaaaa agtgagtatg cttttgactt ggaacaagtc gcacgtgttt ggaatcatgg
     1921 ttcggtgatt cgcggctggc tgatggaaat catggaagat cagttaaacg aacatcccaa
     1981 tttaagtaat tatcggggaa tcgtttctgc atcaggtgaa gcaaaatgga cggttgaaac
     2041 agcgctagaa atggctgttc cagttccagt aattgcaact agtttgttca tgaggaatct
     2101 ttcacaggaa gaagatagtt tttcagcaaa agtagtttca gcactaagaa atgggtttgg
     2161 cggacatgaa atagttgaaa agggtgagta aaatgccaga ttatcatata gcaatcgtta
     2221 attccagcag tttcggaaga gtctttccac accacttgga aagattaaag tcagttggag
     2281 acgttcattt ctttactgtt gatcaagaga ttccaggaaa agaactagcg gagttactta
     2341 aaggattcaa tatcattatt gcaagtgtta cgccattttt tcgacgagat ttttttgaaa
     2401 acaaagatga actgctattg atttcccgac atggtattgg ttacaacaat attgatttaa
     2461 aagcagcaaa agaacaccag acaatcgtat ctatcatacc tgctttagtt gaacgaaatg
     2521 ctgtagcaga aaataatata actaatttgt tgtccatgct tcgacgcacg acacaagcac
     2581 aagcaagagt caaggacgat cgatgggaag atcgggcgga gttcgtcggt aggacgctgt
     2641 ttaacaaaac agttggtgtg attggtgcag gaaataccgg aagcggagta atcgaaattt
     2701 tgcgtaacgg ttttcgctgt gatgttctag cttatgatcc ttacaaatca gatttataca
     2761 tcgaaagctt tggtgcaaaa aaagtagaac ttcaagaatt actggaacaa tcagatatca
     2821 tttgtttatg tgcgaatttg acagaagaga actatcacat gatttctgat aaagaagtcg
     2881 atcagatgaa agaaaatgtt tatctatcta atagtgcaag aggcgctcta gttgatgaag
     2941 aagcgatgat taatggtctg aaaagcggaa agatcgcagg atatgccaca gatgttttag
     3001 aagaagaacc gggacgtcaa gatcatccgt atttagcgtt tgaacaagta gtgattacgc
     3061 cacatatatc ggcctacaca agggaatgtt tagaagcaat gggtgaaaaa tgtgtcagag
     3121 atgtcgaaca gatcagtcag caccttcttc ctgaacgttc tgtccagaca aagagcaact
     3181 atataaaagg atgatgaaga aaatggtgaa cggtttaaaa gtgaaaacag gccctcaatt
     3241 ttatttatat gaagaaggtg gtatttcaaa agtttctgat ctcttgaaat catacggcgc
     3301 gaaaagagta ctggtcaccc acggaacggt atcatgggaa aaggcgctac ctaaattagt
     3361 atttttgaat gacgaaacga ttcaattttt ttatcatcgt tattctggag aatgtagtta
     3421 tgctgaagcc agacgaatag ctacaatcat caaaaaaatg aaatcgattt cgtagttggc
     3481 gttggcggag gaaaactgac cgatctagtg ggttatagtg ccaatcttgc aagaatcgat
     3541 ttcggagtca tccctacttt agctagtaat tgtgctccat ggacacctct tgctgtcatg
     3601 tataaggaga atggacaagc agaagggaaa acagaacact tttacagaca agctgctttt
     3661 tttataacag atccccaatt agtgattgat tcccctgttc catatttcat tgccgggtta
     3721 gcagatacac tagcaaaatg gtatgaatct gagctcatct tagaacaaga ttttttgcaa
     3781 gatgaatcat ttttgaagct agcacaagat actgcgaaga tctgcaaaga agagattttg
     3841 aatcattctg ctaaagcaat ccaagatatg gcacaaagaa aactgacatc ggagttcaaa
     3901 cagctttcag agattatctt tgctgttgct ggtttagttg gaggattagg agataaatac
     3961 gcaagaaatg cggcagcaca tgctatgcat gacgctatca gtaaatatat tccggaaagc
     4021 catcgttttc tacatggaga aaaagtagcg tatgggattt tctatcaact ggcactagaa
     4081 ggtcgctggg aaaaaataga cgagttatat cctttgtatc gggaattaga actgccggtt
     4141 tctctagaac aaatgggatt atttccgaaa gataaacaga taatagataa aatggctgaa
     4201 tttattgatt cgaaagagaa agtgcatttg attcctatag aggtaacaca gaaatcactg
     4261 atacagtcaa tgatggaact ggaaggttat atcagaaaga tctgaaggag gaggacacat
     4321 ggaaatatta agtgtagtac aaagtttgtt gattgcttta tgggtagccg cggttatgtc
     4381 ccgctggtta ggtggaggag ccacgttgac cttacgtttt tctcctttga tgactggatt
     4441 agtcgtcggg ttgatcatgg gggaagttgc acaagcgatg attgttacag ccgctttgca
     4501 aatgatttat atgggggttt tttctccagg aggatcaatg ccagctgaac catctattgc
     4561 agcagctatc gcagtaccag tcgcattatt aggggatctg tcgccagaag cagcgattgc
     4621 cgttgcggtg ccagtcggat tgctcggaag ttacttgtat cagtttcgtt ttttcatcaa
     4681 tactttttta ggtaaatata cagacaaagc tgtagaagat ttgaatacaa aagctatggc
     4741 ccgatcaatt attctttatc caacggttgc ctcattttta ttattcgttc cgcttgtttt
     4801 cattgcttta tatttagggg cgccattgat cgctgatgta atcaaagctt tagagggaac
     4861 agtcgttatc cacattcttg aagtagtagg tggagggtta gcggcaatcg gtatcgcgac
     4921 aactgtatac gttatcggtc gaaaagatta tctggtcttt ttcttccttg cttattttat
     4981 gagtgttgtt ttgaaatctt tagatattac gatggttact tatgccattt ttgggattat
     5041 tatcgctttg atctttgtcc agtcgcaaaa agggaaacaa gtacagcctg ccacaagcaa
     5101 taacaattca gcatcttttg atgacgaaga tgactatgat gacggatttt aatttaggag
     5161 gaatgggaaa tggctgaaat aatggataat caaacaggtg ctcctgaaga aatcacgaaa
     5221 aaagatgtaa caaaagccta tctgagatgg cattttgcaa acgaaattcc tcactctttt
     5281 gaacgttact tggcgccatc actactatac ggaatgatgc caatcttgaa aaaactttat
     5341 aaagatgaag actctttaaa agcagcatat aaacggcagt tactgttctt caacactcaa
     5401 ttaagctggg gtggcggtgt gattactggc ctaatgtctt ctatggaaca gcaaagagca
     5461 gaagaagagt atgaagaaaa agagatcttg atgcaggatg acttgatgta taacacgaaa
     5521 gcgggattga tgggggcact agcaggaatc ggagatgcaa tcgattcagg aaccgtccaa
     5581 tatattttta tcgcaatcgc ggtaccttgg gcacaacagg gaagtccttt aggcgcactt
     5641 tttcctttta tctgctttgg gttgtatcag gttttactag gggtattttt cgcaagacaa
     5701 gctttcacta tgggccgtaa tgctacaggc ctaatgcaaa acactggtgt tcagacagcg
     5761 attgaaatgc tatcagtttt agggttgttt atgatggggg tgcttgcggg aaactatgta
     5821 aaagtagaat cttcattgaa atttgcgata tccggacgtg agtttgttat ccaggaaatt
     5881 cttgatcaaa tcgtgccagg gatattacct ttagctgtcg taatgggtgt gtacttgttc
     5941 tatacgaaaa aaggattgaa agtcacacaa gcattattat ggctgactgg aatattaatt
     6001 gtgctagcag gaatcggtat tttataaaaa gaagggaacg tgaataagat gggtgtagtc
     6061 aatttagcgc gtgtggatga gcgtttgatc catggacaag taatggtaac gttatctcaa
     6121 aaaagtggag tgaattccat ttttgttgta gacgaagttg tagcaaaaga taaatttatg
     6181 agagatcttt acaaaaatgc aggaagccgt acaggacaaa aaacaattgt cattacacca
     6241 gagaaagcta aattctattg ggatgaatat caatttaaag aatacaattg tattttgatc
     6301 gcaaaaacag tccaaacgat ttacgactta gtaaaacacg gaatccccat ggaagaattg
     6361 aatattggag ggattgctca aaaaaatcct gatacagatc ttttagtgac gaaatctgtt
     6421 tatttaaata aatcagatgc tgaaaaattg aaagaaatga atgaacaata tggcgtaaag
     6481 aatatttatt ttcaagccac tccatctgca cctaaaacag gattaaaaga tgtattgaac
     6541 aagtttgatt tgtcataaac aaatgcttag aaaaaagata aagagggcga aaaaatgaag
     6601 aagaacgagc tgtttcgtga ttgggaattt cgttatcgct atatttaccg taagcgccgt
     6661 acaaaaaaga gtaaacagcg ttttctttca gcattagtat cagatattta cagtatgaga
     6721 acagatgtga cagtcattgc ttacgatacg ctagcctacc gatcaaaaaa tatttatgtt
     6781 ggagatatcg aaaaagcaga gaaagttatc tgtacttatt acgatacacc cgttcatgca
     6841 ttaggaagtt atttcatgtt tgattggaaa gaccaaagga aaaaaacaat ctactctata
     6901 ttattgtcct tcattttgtt gttcagttta ggttggtggg gaatgatgat atataacaaa
     6961 aatcctcatc acgtattcga tctgctatcc gtataaacag gtatcactgt actagcattt
     7021 gggagctact ttttcttatt gggaaaagca gctcgcggat ggcctagcag acaaactttt
     7081 attcgaaata cttcatccat attaacgatg ctagaaatga tccgtacgat tgatgatccg
     7141 agtgtagcgt atgcgtttgt tgatgaaggc tgttatggag aaaagggctt agacagcgtg
     7201 agatcgggta tgaaaaaaga ggctattctt ttttatctag actctgttgg ggcagataca
     7261 ccgctacagt tttcgggaaa ttatttttca aataaagaac agtggctgaa acaagttgat
     7321 aagttgaaag aaaaaaacgt caattatatt tttagtgcaa gaaaaaagca agcgcagttc
     7381 ttctatttga ccaaaacaga tttacgaggg aaaacattta attggcaaaa tgccaatcag
     7441 attattgctc tttttcgata agtaaaaagt gcaaaacaag gagtgagaag actaatgttg
     7501 gaaatcatga tcgttacaca tggtaagttg agcgaaggac tgaaagatgc ggcggaagta
     7561 attgtcggga catcgagcca tattgaaaca atgtgcttag agtctgggga ggacatccaa
     7621 acattcggag agaaggtcaa agaaaaatta actatgatca acacagcaga acccatgcta
     7681 gtatttgttg atctaattag cgcaagtccg tacaatcaag cgctcttggc agtcaatgaa
     7741 ctgactgaag aaaagaaagc agccacttat gtcatagggg gcgtgaatct gccaatgttg
     7801 ttagaagcaa tcaatcatca gatgcttcac acaccgatag aagaagcggt ggaacaaatt
     7861 gtcaaacaag gaaaagataa tttaactgtt tggcatgctt cagcggaaga aaaaaaatta
     7921 gatgaggacg atttttagaa ggaaggctga aaagatgaag tggggattta gatggtatgg
     7981 tgaaaaaggg gattcgatcc cattaaaata tgttcgccaa ataccgggaa taaatgggat
     8041 aattggaact ttgctgacta aaatgcctgg agatgtttgg gagatttcag aaattgagga
     8101 gttgaaagca tctgttgaga gccaaggact ggaactgctt ggaattgaaa gtgtagcgat
     8161 ccacgatgca atcaaggcag ggacagatga acgagattac tatattgata actatatacg
     8221 aacaatcaaa aacttgtctc gatgtggggt tcgattgatt tgttatagct tcaaaccaat
     8281 ttttggatgg gcgaaaactg atttgtttaa tgaaaacaac gatggaagct attctctttt
     8341 atacgatcag aaaatcgtcg atcagatgga gccaggagaa atgtatcaat tgatacacag
     8401 ccagtcaaag ggattcaaat tgccgggttg ggaagaagaa cggttgaaaa aattcgctca
     8461 gctggaaaaa ctgtatgaag gaatgaccga agaagaatta tttgaaaatc tgacttattt
     8521 tttagaaaaa gtgattccag tgtgcgaaaa agcagatgta aaaatggcta tccatccaga
     8581 tgatccgcca tgggaacttt ttggttttcc tagagtgacg aaaaatcttg gcgacttgaa
     8641 gaaaattcta aatgcagtag attctccata taatggacta acgctgtgca caggatcttt
     8701 aggggcaaat ccggaaaacg atttagttga aatcatccat gaggtaggtt cgcgcatcca
     8761 ttttgtccac ttcagaaacg tggagttttt aggagaaaga aagttcaaag aaacggcaca
     8821 tccctctaca gaaggctccc ttgatatgta tcatattatg aaagcgttgg tgcaagaagg
     8881 gttcgacggt cctatacgcc ctgatcatgg tagaaccgtg tggggtgaag tggctatgcc
     8941 tggatatggc ctgtatgatc gtgcaatggg actcacttac atgcaaggtc tatatgaggc
     9001 gataagtaag gaaaaaccac tgggagagca ttatgaataa aatcaagctc ttcatctttg
     9061 atatggatgg ccttttatta gaaactggac gtttagctta tcgtgcttat gtaaaatcag
     9121 cacaaaagta tgattatgaa atgcgtaagg aagtctatta tttactaact ggccaaacag
     9181 aaatggcgat aagagaacaa atgggcattt tgtacggaga agatgttcca tatatcaaat
     9241 ggcgagaagc aatcaatcaa tataaagaaa aaatcgtgaa agaagataag agagtatata
     9301 cgaaaaaagg agcagaagaa attttatctt ttgcaaaaga aagaggaatc catacaatcg
     9361 tggcttcgtc taatacacgg gtaaaagtag aaatgtattt aaaaatggaa aatctatatg
     9421 ccttgtttga tcaaatcatt tcaggggatg atgtaaaaaa aggaaagccg gaaccagaga
     9481 tctttttgaa agcttgttca aaaatgaata ttcctccaag tgaagcactt gtatttgaag
     9541 actcgattgc agggatcgaa gcagcaaggc gagcgggcat tttgtctttt ctcatcaaag
     9601 atcatttgat agggttgccc gatcataatg ggaagcataa gttgaaagta gatgtaagtt
     9661 tatatgaatc caataaagca gactatgaat tttatgacct gcatcaagca aaacgatttt
     9721 tagaaagtaa agggttgtat ctctaataaa gaggactatt ttctagtcct ctttatttat
     9781 tgtcctgcac gactgaggga acagatgaaa aagaagtaat tttttatttt gaagtatcta
     9841 ccttatttgt agtgaacaat ttagaacaaa cacaaaacgc atctactgtt atctaccagt
     9901 agatgcgttt aaagagaaga acgagaatcg tccatatgag ggtttccacg gctctataat
     9961 ttctaggact gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat
    10021 acagaagctc cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct
    10081 gatcttcatc atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat
    10141 gtggaatcga agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa
    10201 gaaattcgtc gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac
    10261 ggaaagaaaa aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct
    10321 ttagcgaaac aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac
    10381 attgtcgata gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta
    10441 actgaaaaac aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt
    10501 tctcggacac tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt
    10561 tgtttattaa tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct
    10621 tgtatggatg gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct
    10681 ctcgtttctt attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg
    10741 acggacatga atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc
    10801 gttgtggatc gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta
    10861 cgaatcatga aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta
    10921 aaggcgttgt ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc
    10981 aaatggcgca attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt
    11041 tcgatctctg acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt
    11101 cacgacaaag aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca
    11161 aaagaactgc attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc
    11221 ctcgaattgc cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg
    11281 aaacgagtcg cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta
    11341 aataatctga ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag
    11401 atgttcttat ctcactctat cagtcctatt tgacaaagag ccgtttccac tccttatgca
    11461 gattttttta attcttgcac tgcttgttcg atcgtaacac ctgtcgctac atgattggta
    11521 ttttctttgt caaaaacgac aaatagattc aaatctgtat ccaaggttac tcgatattgt
    11581 atatctttgt tatagaatgt catagccatt ctcctttctt taaatacttg ctgaaaggca
    11641 acgaacgcgt tgataacgaa attccattta taagtatacc accaattgaa aggttttcat
    11701 agttaagaaa ttttaaagtc aaaagttgca gataaaagca aataaacaag aaaacaaaaa
    11761 gtgaatcaat cgcggttatt caactttttc ttcgattcga agacaataga agacaatgaa
    11821 ttttatattt cattg
//
LOCUS       DAZKCZ010000077        11686 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000077 DAZKCZ010000000
VERSION     DAZKCZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.078"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8KN_001921"
     CDS             complement(<1..132)
                     /locus_tag="ACG8KN_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9188969.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="ACG8KN_001922"
     CDS             542..841
                     /locus_tag="ACG8KN_001922"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9188970.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="ACG8KN_001923"
     CDS             914..2275
                     /locus_tag="ACG8KN_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9188971.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="ACG8KN_001924"
     CDS             2297..2557
                     /locus_tag="ACG8KN_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188972.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="ACG8KN_001925"
     CDS             2571..3323
                     /locus_tag="ACG8KN_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFP9188973.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="ACG8KN_001926"
     CDS             complement(3417..4022)
                     /locus_tag="ACG8KN_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9188974.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(4078..5052)
                     /locus_tag="ACG8KN_001927"
     CDS             complement(4078..5052)
                     /locus_tag="ACG8KN_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFP9188975.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5430..6149)
                     /locus_tag="ACG8KN_001928"
     CDS             complement(5430..6149)
                     /locus_tag="ACG8KN_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9188976.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(6276..7547)
                     /gene="celB"
                     /locus_tag="ACG8KN_001929"
     CDS             complement(6276..7547)
                     /gene="celB"
                     /locus_tag="ACG8KN_001929"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9188977.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7700..9097
                     /locus_tag="ACG8KN_001930"
     CDS             7700..9097
                     /locus_tag="ACG8KN_001930"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9188978.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9178..9618
                     /locus_tag="ACG8KN_001931"
     CDS             9178..9618
                     /locus_tag="ACG8KN_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9188979.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9765..10091
                     /locus_tag="ACG8KN_001932"
     CDS             9765..10091
                     /locus_tag="ACG8KN_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9188980.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10092..10841
                     /locus_tag="ACG8KN_001933"
     CDS             10092..10841
                     /locus_tag="ACG8KN_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFP9188981.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10867..11277)
                     /locus_tag="ACG8KN_001934"
     CDS             complement(10867..11277)
                     /locus_tag="ACG8KN_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9188982.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3732 a         2043 c         2390 g         3521 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttcgaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc ctgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttattgc tgcttttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttaccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcctcacga tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat tcagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag gaccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact caaaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg ggagatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgctcga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccaatcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaaaaat gaagtagcat gaagagatta
     4081 ttcacgtttt ttatttgcat cacttgtatc gaaaggacgg acataggctt caatggcttc
     4141 acgacgttct tctaagaatg gaggaagaga tagtttttct cctgctgatt ctgctggttc
     4201 atcttcaagg aatccaggac aatcagttgc taattcaaac agtacgtgat gagcggctag
     4261 gaagtactca gattggaaat agaaacgatc gacgaaacca gaatttggca attcaatctt
     4321 attgattact tgaatccagt attccaatgc ttctttgtct gctacacgca atgctaagtg
     4381 atggatattt ccaaatcctt cgattgcttt tggcaagtct gtccgatgtt caataatgat
     4441 gcttgcacca tttccgcctt caccgatttc aaataagtag aaatctcctg aatggtctgt
     4501 ttctttgaat cctaagattt tttcaagaat caagcggata tgctctaatt gactgacacg
     4561 gacaaagaca ggtccaagtc ctgtgatcgc aaactctgtc ggtacattgc tgtttttcca
     4621 aggaatgcca gctgctacac cttgattgtt ttcgtcagag atcaattgat aatactgatt
     4681 atcgaaatct tcaaattcga tatatttttt gccgaaacgt tcattgattg ctgaatgagt
     4741 aacgtcatat ttttcaaaac gttccaacca aaagtctaat gctgcgtcac ttggcacacg
     4801 gaaagaagca cgagagatcg tatctgttcc tttttttcct tttggctgat ttggaaaatc
     4861 aaagaaagtc atatctgttc ctggcgcacc agtatcgtct gtaaagtaaa gatggtaagt
     4921 ttcgatgtca tcttggttaa ctgttttttt gattaagcgc attcctaaaa tatctgtaaa
     4981 aaagcggtag atttttgtag cgctagatgt cattgctgtg acgtggtgaa ttcctctgat
     5041 ttgtgtatcc atgataaatt tcctcctgtc aacttacaaa aagtaagtag ttgatttaga
     5101 catagtatag agggtgcatc tctattttgc aattaatttg tcttctaaag tatcaggaag
     5161 gaatcaagaa tggaaaagag gagggacgga tcaataaaat aaaaagactt acgactagct
     5221 gtatcgattt caaaaaaact agctttaaaa agctaacttt agaatcatta cgggtggtca
     5281 taagtctttt tatgctgagt tattttttca tttttgagct aatgcttagg ataggataga
     5341 atgttgattt cacgatctct tcttgttact tgaaactgct tactactgtc acgatctctt
     5401 ggtaaacggc tttttcacaa cctcttcatt taagttgaac gcacatccag cacagaatag
     5461 ttccgaacat caaatcggtt tcggctggaa gaatattcaa tcggctgtcc atctttgaga
     5521 tagacgactt gctgaacttc cagtacagga tcgttttctg cacagttcaa atattgctga
     5581 tcatattcat cacttttatc tgcagaaata gcacgataag cccctgcaaa atggacattc
     5641 aattcttgat gaagatgatc atagatagag gctaacaaat gttctttttt taaacccggc
     5701 acaagatgga ctggcataaa agtatgctct aaaacatatg gctcgccatc taagatcctt
     5761 aaccggtgaa tattgtagac aggttgctcg gcatcgatca tcaatcgttc ttggatatat
     5821 tgattaggga attcaacatc aaaatgaatg atttgactct tcaaagaacg atgtctgcgt
     5881 ttcatttgga gactcaaacc ttcatactcg ttagcgggcg atgtgtcttt ttgcataaat
     5941 ggatgattca agattttcgt tcctgatcct cgctgcgaat agacaagtcc ttccattgct
     6001 aagatattga tggctttttt gattgtcatc cggctcgcac caaattcttt gaccaagtcc
     6061 acttgattag gcaaaaaact atcgggagga tactcttttg aacggatacg ttctcttaaa
     6121 atatttgcga tttcttcgta ttttggcacg ttattcacct cttctagagt ataacgaaag
     6181 attatgcttc cgacaactcg aagtagcatc tggaacattg tttattcgaa aaaagaggtc
     6241 gtgacaagtt tttgtcacaa ccccttaatg atctattaag caatatctaa atctggttgt
     6301 ttatctagta ctttcaggaa tgggtaccag ataaaggcaa cgatgacaaa gtcgatgact
     6361 tgcagcatac caccaagcca tgaatttgtt gctaagacac cgcttgcaat gattggcaca
     6421 gtccatggaa cagagacgcc agttggtgca gggacaagtc cagcagccat taccaaataa
     6481 ttgaacgtcg ttacgattag aggtgccaca acccaaggaa taaggattgt tgcattcaat
     6541 acgatcggta gaccgaagat cgctggttca tttacattga aaataccagg tgctaaagct
     6601 aatcgtccaa catcacgata ctgtttcttt ttaagaacaa aggccatcaa taaaacgaca
     6661 gctaaagtca ttccagagcc accaatacca acagtgaagg tttccatgaa tggttttgtg
     6721 atgatgtgtg gtaattcttt tccatgttga taagcttcta agttatcaag catcagtgta
     6781 ttccaaatcg ggtccattac agagttgacg atgatttgtc cgtgaagtcc gaagaaccat
     6841 aagaattgga caaagaacaa agcaatcaat gttgcaggta aactacttcc caaaccagtt
     6901 aatggttttt ggatcacttc ataaacgacg tcatgtaagt tagcattgaa aataccagtc
     6961 atcacagcat tgattactaa gaaaatagtt agtgtcacta cagcagggat caaagcggca
     7021 aatgattttg ttactgcagg cggaactcca tcaggcattt tgatctgcca gccgcgtttc
     7081 gtgattctgc agtagatttc tccagccaag aaagcagcga tcattccaat gaacattcct
     7141 tttgcgccaa gacgatcaag tgaaagcaca ccagtcacat cttcaccgcc agcggttttc
     7201 attgcaaaag gcgtaagaat caagaagctg gcaaaagaca cggcaccacc gaaaatacct
     7261 tcaacgtcat aagattttga taaataatag ccgataccaa aagtaacaaa aaccgacatg
     7321 atgctcattg ttgcattttg cccattaccg aataagttcg ataaagttcc ctttgttgcg
     7381 tcactgaaaa aaggcaagtt attgataacg acaacgatcg aaccgaacat cgttaaaggg
     7441 aaggaaagca tgaaagcatc ccgcagcact gtaaggtaac gattttgccc taataaatta
     7501 gctaagggca tgattttctc agaaagcttg tccataaaac cgttcatttt tattcacctc
     7561 aaatttattt gcaatcgatt acatacaaag tataacgcca aaaattttaa atgtctagtc
     7621 attttcataa aaagtattga tttatttttt agggttcgtt atgattacga tatcaagaaa
     7681 aaagagagga agtaaaaaaa tgaaaaccta cacattccca gaaaattttt ggtggggttc
     7741 tgctgctagc ggaccgcaaa cagaaggaag agtaagtgat gatggtaaag gagaaaatat
     7801 ttggaatcat tggtatgaat tagagcctga aaaattcttc gatcaagtag gacctgaaaa
     7861 aacttcgcaa gtttacacaa aatacaaaga agatgttcag ttaatgaaag aaactggcca
     7921 taacacattt cgtacatcta tccaatggag tcgtttgatt ccagaaggga caggtgaaat
     7981 caatcaaaaa gctgtttctt tttatcgaga ttattttact gaattgctgg ataatggtat
     8041 cgaacctttt gtgaaccttt accatttcga tatgccacta ccactgcaag aaaaaggcgg
     8101 gtggttatca agagaaacgg tcgacgctta tgtttcttat gcagcagcgt gctttgaact
     8161 gtttggagat ctggtcaaaa aatggtttac tcataatgaa ccaatcgtac ctgtagaagg
     8221 aggctacttg tatcaattcc actatccaaa tcatatcaat atgaaagaag cggtccaagt
     8281 aggctatcat gagaatctag caagcgcaaa ggcaatcaaa gcctaccatg aaggcggaca
     8341 tgatggtgag atcggaatca tcttgaatct gacaccaagc tatccgagag atgaaacgaa
     8401 cccagaagat gtaaaagcgg cgaagatcgc tgacgcattc ttcaaccgat cattccttga
     8461 ccctgcagtg aaaggagagt ttccacaaga gttgatcgaa attgtcaaag aattggctat
     8521 aatgccagag atccatgaag gagacctaga actcatccgt gaaaacacga ttgacctgct
     8581 aggaatcaat tactatcagc ctcgtcgaat caaagcaaaa gaaactccaa tcaatcacga
     8641 acatagcccc atgccagatg attattttga taattacgac atgcccaata aaaaaatgaa
     8701 tccatatcgt ggatgggaaa tttacgaaaa aggaatctat gatattttaa cgaatacaag
     8761 agaaaactat ggtaatatca aatgctatat ttctgaaaat gggatggggg tcgaaggaga
     8821 agaacgattt gttaacagcg aaggaactat tgaagatgat taccggattg agtttgttac
     8881 ggatcatttg aaatacgtcc atcaagcaat ccaagaagga accaattgtg tgggttacca
     8941 catgtggaca tgtatggaca actggtcttg gacaaatgct tataagaatc gctatggctt
     9001 tatcgctgtt gatttggcac aagacggtaa acggacgatc aaaaaatcag gtcgctggtt
     9061 taaaacggtc tctgaaaaaa acggatttga tgcctgatat tttttgataa aacgttcaca
     9121 aaatgtttgt tctaacactg taatcgtgct acactaacag tgaaggaggc gtttacaatg
     9181 gtagatactt atcgtaatat tttagtagca attgatggtt ctgaaaaggc agaaaaagca
     9241 tttcttgagg caatcacgat tgccaaacga aatcaggcaa cgttgcacat cctgtatgtc
     9301 aatgaagtaa caggcaatta ctttggtgat tttgcatttg ttacaacaag ccttcaggaa
     9361 gaattggatg aggttgcgga aaaccagatg aaagaacatc gaaacttggc aattgaaaaa
     9421 ggcttgacag atatcgagac atatgttctt tacggttatc caaagacact gattgctaat
     9481 ttccatgaat cgaaagaacc aatcgatctg attgtcatgg gggcgacagg tctgaatgcg
     9541 gtagaacgtg cactagtagg ttccactact tcctatgtag tcaaccacgc atcatgtaac
     9601 gtcatggttg taaaataaat aaattaaggg aacagaaagc aaaaaagcat aacgcttctt
     9661 ttgctttctg ttcctttaat tttttgtaaa aaattacttt acctgatgaa gtaatcatgc
     9721 tattctaatt acaacgacag atggagtaat atggaggaaa gaaaatgatt tcaagtgatg
     9781 gaatacgcgg atacaatgat gcgattattt tatcgatcct taacaaaaac gattcttatg
     9841 gatatgaaat ttctaaaacg attcgtgaga ggacaaatga ttcctatgtg atcaaagaga
     9901 caacacttta ctccgttttt gcccgtttag aaaagaatca actgattgtt tcgtatcctg
     9961 gagaagtgac tcatggaaaa aagcggacat attataaaat cactgaaata gggcgtaatc
    10021 ttttagaaga aaaaaagcaa gagtggctga cgacgaaaga cgtagtcgat caatttttgg
    10081 agggaaatta aatggataca atccgtaatt atttagacag tctttttatc ggagtacctc
    10141 aaagtaccga gatagataag ctgaaaactg atttgttggc aaatatggaa gaccattatc
    10201 atgaattgat gggagaagga aaaaatgaac aggaggccat cggtacagta atttcgacat
    10261 ttggttcaat cgatgaatta ttagaagaac tagatgtcga aaaaaaacat caagctgacg
    10321 agacggaaac aaacacagct tctatttatt taagcgaagc agaaaactac tggaaagaat
    10381 acagagcggc aagtctccaa gtggcatcag gtgttctatt cattagctta tcttttgcaa
    10441 gttttctttt cttttgttca gcagggtatg tgttcatggg tatttcttgt ctgattttcg
    10501 gtatagcatt agctgtcggt ttttttattg cttctggtat gaaaatcaca cgtttaaatc
    10561 actttttgca ccatcggaaa atacctgaaa aagtattagc agaagcaaaa gaaaaagaag
    10621 aagagtatca gcgaagtttt ggcttctcac tgattgcagg aattggatta tgtatttttt
    10681 cacttttccc acttttggct tctttaatgt ggtatatgga tggtagtatc ggtgcctcta
    10741 tattttttgt tacggtagga acgggagtct tcttgatcat ttatggcagt ctggtccgtc
    10801 attcttatcg tcaattcact caaagtgctt attactggtg aatgtattca gatttgtaaa
    10861 aatattttat ttattgctta cccatgtaaa atcgttatag acagatttca gttgacttct
    10921 gcgccattgt acagggataa aaagacgcat actatcttca ttgaatccga ctaataattt
    10981 ctttccgtca gtaatgatgg gacgttgtaa caaagtaaaa tgcgagacga tcagatcgac
    11041 aagctctgag gtcgtacatt ggtcgatttg atctttcaaa tttttataat caatcgagcg
    11101 tttagctaat agatcgtcaa atccgttttc tgttaatgac aataaataaa atatctcttt
    11161 ccttgttaaa ggctgtttag gtgtgaaaat tcgttctctg taaggcattt gatggtcttt
    11221 cagccagttt gccatcagat ggtaactatg tgtgcgagaa gaatagatcg taagcataaa
    11281 aaaactcctt aaatagtcca ttaatttgtt agatctgttg agataaataa taggtaagga
    11341 gctgtgcata agcattgatc aaaacaaccc aaacatcatt agaaataact ttctaaagtc
    11401 ttaaacttgc gacgagaaac aactagacta atagaaaaaa tgtcttagcc atttggcttc
    11461 ctgtttgttt ttttgcttat gctgcagctc ctcgggaaag ggttctaaca atttgaagaa
    11521 ggttgatgat tgatctagtt taaattgtgt gaaatttgaa atctggtgtt tttactagat
    11581 gaatcaccta acaactatga ttataactta tagaacttct gttttgtact tggttttgtt
    11641 atagttgtta taaagtttta ttttattaca gaacattttt aattaa
//
LOCUS       DAZKCZ010000078        11658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000078 DAZKCZ010000000
VERSION     DAZKCZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.079"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..528
                     /locus_tag="ACG8KN_001935"
     CDS             322..528
                     /locus_tag="ACG8KN_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFP9188983.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(730..1731)
                     /locus_tag="ACG8KN_001936"
     CDS             complement(730..1731)
                     /locus_tag="ACG8KN_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9188984.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1736..3649)
                     /locus_tag="ACG8KN_001937"
     CDS             complement(1736..3649)
                     /locus_tag="ACG8KN_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9188985.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3817..4323)
                     /locus_tag="ACG8KN_001938"
     CDS             complement(3817..4323)
                     /locus_tag="ACG8KN_001938"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9188986.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4483..4923
                     /locus_tag="ACG8KN_001939"
     CDS             4483..4923
                     /locus_tag="ACG8KN_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9188987.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4949..6064)
                     /locus_tag="ACG8KN_001940"
     CDS             complement(4949..6064)
                     /locus_tag="ACG8KN_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188988.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6109..6465)
                     /locus_tag="ACG8KN_001941"
     CDS             complement(6109..6465)
                     /locus_tag="ACG8KN_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9188989.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6763..7737)
                     /gene="sgrA"
                     /locus_tag="ACG8KN_001942"
     CDS             complement(6763..7737)
                     /gene="sgrA"
                     /locus_tag="ACG8KN_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFP9188990.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7933..8772)
                     /locus_tag="ACG8KN_001943"
     CDS             complement(7933..8772)
                     /locus_tag="ACG8KN_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9188991.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8957..9844
                     /locus_tag="ACG8KN_001944"
     CDS             8957..9844
                     /locus_tag="ACG8KN_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9188992.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKDGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10438..11133)
                     /locus_tag="ACG8KN_001945"
     CDS             complement(10438..11133)
                     /locus_tag="ACG8KN_001945"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9188993.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11117..11515)
                     /locus_tag="ACG8KN_001946"
     CDS             complement(11117..11515)
                     /locus_tag="ACG8KN_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9188994.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3478 a         2314 c         1744 g         4122 t
ORIGIN      
        1 ttatcatttg taattttatt tcacagttga tatgattgtt atgtaaaata aaaaagttta
       61 ggatacatcg aatttttggg gaaattcttt tgggggtatc ctaaatcttt tctattatat
      121 tcccatattt tctattattg gtctagacgt atgatggtaa actttgtacc tatctgattg
      181 ctgaatcatt aattgcttat cattagccat ctttgtttca agttcgctag atgatcattt
      241 tattcacatc ctaacaaact tgtcacattt atagattctt aatctattta tcatacaatt
      301 ataaaaaagg aggtgctgct tatgaaagct atccatattt ctttgctttt tttcgctagt
      361 gcattgattt tatttgctat tcatctttgt tcccagcctt atatcaataa caacggaata
      421 ttagtggaac cagcattctt ttgcttacca ctcggctttt tgtcccttct tgcctccgca
      481 ggcttcggat ttgttgcttt ttttaaacga tcaaaacaac aaatctgact tataaaattt
      541 acgtaaaaga gatcgtgaca ataatcgtgt gatattccca aatttcgaat aaaaggtgcc
      601 ccttgccatc tctaactcct cagtcacaag ttacaatatt cataaaccat gaaaagcttg
      661 tttattgaaa ttgctccttg tgacttggag ctaagtgaca gattacaacc tcttttgcat
      721 cataagactt tactccttga aaaacattac taacttttga tatgtttctt gcgcttgttt
      781 attgctgaaa tcaaactgat attcatggtt caaaacagca tttttctccg cataatctac
      841 ttttgtgact ttcacccccg ctttctcaaa tacagaagtt acttcttcca tttgtttcgt
      901 aaacgtattt gtatttccat ccgttaagaa tgtgttttgc gtccaacgat ccgcatgtct
      961 gaccaaggct aaacttttga ccttctctga ttgttcaaag tccttattat caaagtaagc
     1021 ccatccacat ttttcataaa gaaagctaga gattttatca cttacttgag taagtgcaga
     1081 aggatccaac agaccagaca aagaaataaa cttcgtaatc tgcttgttct ccaaagtcgg
     1141 atcaagatga tcagcttttt taatttcttc attttgaagt gctaacaaat attctccact
     1201 aatttgtgca cctgccgaat ttccaccaat cgaaatattc gaccaatctg cttgatcttt
     1261 tatctctttt ttagctgctt gaatggcttc gttcaattgg ataactggtg taggatgttt
     1321 tgcttccggt gatcgttcat aatctatatt tacgatgatt tgctgcgtgt ctgaacatag
     1381 atattccaaa taatcacgac aatcattttt atcccctcct acatatgcgc ctccatgcat
     1441 ccagaaaaga atcggtaatt ttttcttttc cactttcggt gtgtagatat ccatattatt
     1501 atttttgtac tttgaaggat aggatatttc tttttttata tcgatttgtt cgattttttg
     1561 ctggaaatca tctggtcggg catatacttg ttctttttca cttgtgtcaa attgttttct
     1621 aacaagcaag ctaatcggct ttggtgtgat ttgattaatc aaaaatacag tgactaaaat
     1681 aactacgatg ataactggta ctgcaatcac tattcgcttg aaccatttca ttcatttacc
     1741 ctctctttct tcgccaaatc catgtgataa tcgtgctaac agataaaaat ccacctgaat
     1801 aaatgagaag cgaatgtttc ttttctccag ttttcggcaa cattcttttt tttgcatctg
     1861 tctttttttg tactttcact gtcatctgaa tcggttgcgt atcatcactt gattgatcct
     1921 ctcgaggttc attctttccc ttattgtctg cattctctgt cggatcttgc ttttttggtg
     1981 gagctactgg gatttttttg taatagaaaa taatagttgg tatcgtctca ttaaaaatcc
     2041 cttcagtcgg tccttcaaca tttgctaatt cgtatccttc gaatgtcttt tgctctgtca
     2101 tataattttc tccataagct ccgtaaagct ggatttcgtc ctggtctttg agtagacttt
     2161 tattgttttc atccacatac tttacgttta tctgatcatg gaacactact ttcaaaaagt
     2221 ccgttacatg tttcgtgtag gcagcatggt caacttcctg tgcctttcca tgccctgctc
     2281 cttctactaa taaaagatct ttataccctc ctgataactc atataatttt tgcgcgttag
     2341 acacaggtac tacatcatct gctgttccat ggataatcaa tttaggcaat gaatctgaca
     2401 ttccaatcgt aggtagagca gcttttgtat ccaaatgcat ctcctgatca aaatagtatt
     2461 gtttcaaaag gcccataaat aaatcttcat gctcttgatc cccaatgatg tctaactgat
     2521 tttttatggc atccggtagt gcgggggcta ctgtctggct gtacgtaaaa cgtagttgtt
     2581 catcaattga tgcaaatccg caattttcaa taattccttt gacattgaca ggaatatctt
     2641 tcgctaatac agacatcatc gtagctgctc ccatcgaacc accataaagt aaaatctttt
     2701 gagatggccg ctctcgaact tcttgattta tccagtaaag gacgtcatct gactcatatt
     2761 gtccataggt cacataatcg cctccgctat taccagtcgc tctggaatca acaaataata
     2821 aattataccc ggccttataa aaatcattgt attcttctgt aacgatcccg ttattccaat
     2881 tccctcgaaa tccgccatgg atcatgactg ttttatctga accttgatcg acataatatg
     2941 catatacatc catcattttg ccatcttgtt tgtttagtac agtttgatga agttgttttg
     3001 tatttggata gaagcctgtg ttttctgtag gcgttgtccc atcatctgta cctccaagca
     3061 tcgacatcaa cacactgttt tttgcaaaag cgatatcgta caatagataa gtagcaatca
     3121 gatgtttgat aatctcttta tctacaccat aatcattaac agcaagctca gtgactaata
     3181 actctattaa ttttgacttg atgtcttctg ttggtccatt aagtatttct gtcatctgtt
     3241 taccgaagta attcaaatat tctttaccat tgaaattttc acctttaccg atatcatcat
     3301 tttctgtccc attctctttg tcacttactt tagcagaaga ggattcgtcg tttgacattt
     3361 ttgtagctgt tgcttcttct gtttgttggt tatctgtggt agcggtcgtt tcttgatctg
     3421 tgctagtaac agatgtctca accgttgttt cagtatttgt ttctgtttgt gaagtactcg
     3481 atttcgtttc atttccagca tcagtagttt ctgtggtttc tacctcccga gattccgatt
     3541 cggaagaaat gctctctcca tatactgtcc attgcgggac gcttgtactt acagcaaata
     3601 agatgaccaa cgctcccttt aatacttgtt ttgttctcaa gctattcact cctttttatg
     3661 tttctaggag aattatatgt aaacgcaaac attttttata caaaaaataa atggtttatc
     3721 ccataaaaat agaacaggat tattttctca ttttacgtaa taaagcagaa tggataaacc
     3781 agtatccatt ctgctttttt taaacgaaga aacaacttat tgctccaaaa atgtcagaaa
     3841 tcgtttatcc caaattttct cgtaaaaagc gattgtttcc tttgctttca taaaaggatt
     3901 atcataagtt gtcgttgctc ctctacacat cataagctgg tatcctaatc catgagcaaa
     3961 cttcacagtc gtatctatac aatactctgc ttgtgcccca cataattcga tcgaatgaca
     4021 gtccaattca gttaataaaa cactcaaatt agtcttataa aatgaattcg catgggtttt
     4081 tctcacaaaa taactgtgct caggaataga taattccgac aatagctccc atggttttgt
     4141 ttgtggcact aattgagcat cttcgtgttg aacgaaaata actggtttcc caatcgtttc
     4201 gtatgttttg attcttttat tcactaatgc tgtcactgtt tctaattcgt gtatcggcga
     4261 agaatcaaaa catacccctt tttgtaaatc gatcactact attgcgtcgg ctgtgtttga
     4321 catacttgtg ccttctttct tttttcttga tttaatagta tttcttatct agctagttcc
     4381 attatacgtg acaacatttt cttattttac acctctaaat ggcacttttc gactatttct
     4441 tcatgttatt atttcataaa tacttagcta ttggaggaat gtatgatgat taaattgtat
     4501 acaggttctg gctgctcgtc ttcaagacgc gcaaaacgtt ggcttatcgt ccatgatata
     4561 ccgtttatcg aacagaactt aattcgtcaa ccactaacca aaaatgaatt tatccactta
     4621 ctaaaattga caaacaacgg cgtgaccgaa atgctctcta ctcattctat tagttataaa
     4681 aatcttgttc acagaataga tgaactcagt ttgagtgaac tatatattga gattcgtaca
     4741 tccccttctt tattgaaaaa accgattatc tacgaccaat ctgcaaaact tttgactgga
     4801 tttagcgaag ataaaatcag gatgttcctt tctaaaacta aaagaaagca agagctcaac
     4861 gaactcttgc tttctggtca tcttgtcgat tttaaagaaa ataatctttt tgcaatgtct
     4921 taattttggc ataaacaatc tgcttttctt aattaacgaa atccaatttt tccaatatgg
     4981 atctattttt ctcattcctg atttttatga tttcagcttt cagacgcgcc caatcatttt
     5041 ttgtaggttg tccattccag taaaatatct gtgtattcgt ttgatcatat gcctttttgt
     5101 cgataggata atcgctaatg acaatatcag gtaactgatc agcggatgca ctatatttta
     5161 ttgggcgttc agtcatagat aatatccttt tctttgccca taattcctgt gttttaccat
     5221 actttgattg aatatagaat atgacaggtt gttcgtgttt tataaaaata tccctgacta
     5281 acatacagta ttgaaaaatc aaccgtctgt tcttttcata ataagtatta agctctggaa
     5341 ttctgcgagc taccccacta aaaaactgtt gcatgattgg atacatcact ggaaatatat
     5401 gctgaaaatc tctgtcttct gctgattttc caaaagcatc caccttcttt tttgtcccaa
     5461 aaacccttga atacaaatgg atcattgcca aattgacata tagataattt ttttctgaag
     5521 ttgtaaaaca aatcttgaag tttttttcaa aattttctac aatagacgat tcaatccagc
     5581 cccaactttc ttgtacttta tctggagtat ccaacaaatc ttcttccaaa taggtgttca
     5641 ttacaccaaa cataaaataa aggtaagctt gctctgttgg agtgatgtga ttcatcgacc
     5701 agaagtttgc ttcaaaaaaa ggctctagta tgtaatgcgt aaactcttct tttgttatca
     5761 atggatgcac aaatttgttc atctcttctg ggacatacgt aatcgagtgg ccttgtttca
     5821 ttcgttgcaa cgaaattctt agtaggctga tccatttctt tctatctata ctacgcgtat
     5881 atggcgcatg tttcgcaaat ttttctaata gttgacgttt attttttata cttgataata
     5941 tatcagaatg attttcgtcg aatgactgcc aatacatcaa gtgaaaaaag tatcggatac
     6001 ggtattcttc tccttctatc ggattacctt ttttcaagct gcaagataaa tgatagtttt
     6061 tcaaaatagt attaagttct cttaattttc tttgaatcgt acgcaaactt atataatttt
     6121 ttgactaaga ttttctaagg attgataagt acctttgtaa agtgaatcca gtaaaatata
     6181 accaatcgtt ccctctaaaa gtatagaata aactttactc agtaaaaact gagaagaaaa
     6241 ctcgactttt atcatcttgt tttctgaaag aaaagataat ttatattctg gatacctttc
     6301 gcaaaacgat tctgcttgtt gaatcaccga attcatctta gccagggtgt agtttagatc
     6361 taaagcgata tccttcacca catataattt ctcgtctctt tcataacaat agttcaaaaa
     6421 ttgatatact tgcctttcat tgatatcaat aaattcttca aacaaagtac atactccttt
     6481 ttatctatta tttagactta ttccgacagc tacatgaata acgtacaaat agattcgtta
     6541 ctgatttttc agagatacta ccatgcgcag tgtgcattgt caattattct tgctcttatt
     6601 ttccatttgt gttcctgtta tttcctgttt ctcaaatatg ttagtaaaca aaaatagatc
     6661 tggcaaagct acaacttcgc caggcaatac agcactctcg aatgaaatat tcactcaaaa
     6721 atctatcgtt ttattcaatt ttagatctgt tgcttttatt ctttatttca ctcgatggaa
     6781 gagagaacga aaatagcttg ctcctacaat caaaatgagt aacacaccca tccatgtaat
     6841 tagcttgttc aaggttctac taccagtttc tggtaaatcg ctttttctat tatctgtttc
     6901 tttcgctttt tcttgatgat caattccacg agaaacaaaa tcaggctgtt ttagcacact
     6961 ttttaggttg ctccctgcat ttctcttttg ttccttagtt ggtatgatag gtaccaaatc
     7021 aagttcatta tttacttttc ctgtgctgct ttcgctgctc gtgcttcctg tgctactctc
     7081 actactcatg cttcctgtgc tactttcact actcgtactc tccgtactgc tttcgctgct
     7141 cgtgcttcct gtgctacttt cactactcgt actctccgta ctgctttcgc tgctcgtgtt
     7201 ttctgtgcta ctttcactgc tcgtactctc tgtactgctt tcaccgctcg tgttttctgt
     7261 actgctttca ctacttgtgc tatccgtact gcttggcggt ccaacaactg tccaactgct
     7321 tggtttcaaa ttctccgtga tcgtcatctg tccatcataa aaaacaatca aatcaacttt
     7381 atctttactc ttgatctcat caatcgatag ttctgcagaa atcattattg tatactcaat
     7441 accagcggtc cattgtacgt cttttaaata aacttcccca ataacttttt ctgaacttcc
     7501 attaccatcc caaccatatg ttagaacagc atgtacatca ctagcattca cgcttttgtt
     7561 tggtttgact ggaatagtaa tgacttgttg tgtcatgtca taaacaatcc gatcagtttt
     7621 cacagtaaat gaaagattct catttgcccg ttcatttgca gaagtactga ttccaatcat
     7681 tgctgcaatt atactcagcc caagaataac aaataatacg gtcgctgttt tcttcatttc
     7741 tatcactctc catataatta ccttctattt gataaaggaa ttatataaaa tgaaaatgat
     7801 gagaacacga cagtcagttg tgaaaatcta acaaaagatg tcgtgtattc gcttgaattt
     7861 gtttactttt tcgtttaatc aatgacagac ttaaaaaagc aagaccgata tcagccttgc
     7921 tttcttcatc ttttattgat aaacagcagt gtatttcttc atccgctttg ttttatatgc
     7981 atagagtaac aaaccaacaa tcaagactaa caagaataat gtagttgata tagatgcttc
     8041 agccccaaag tttcccccag taattattgt attttgcgat tggatcagag tggagaaaaa
     8101 accttgttct tgcgttccgc tgactggtat acccaaaata gctccttgtg tccaattcca
     8161 agtaatatga gcaccaatag ccgtaaataa acttcctgta ctgtagataa gtgcgctgaa
     8221 aaagcaacct gctaaaaaga tattgaaaac tgccagaaat gtcatgttag gattcaatcc
     8281 gtgcaaaaga gcaaataatg cagcagatcc aatcattgct attaacggac caaatctaag
     8341 catctgatgc tgaaaaaagc cacggaataa aagctcttct gatgccccca ctgcaataaa
     8401 taaaataaaa ctcgttatca gcggcacaat gtttgcgttt cctgaatcta accgatacgc
     8461 ccccgtcaaa tataatggac tagtaataag cagaaacact ccaattccaa caacgagacc
     8521 tcccgctaaa tacccccagt cttttcctcg aacagaaaca ccataatctt ctattctttt
     8581 tccttcgacc ttactagcaa gaaataaagc tagtaaaaaa acagctaaat gtccgaccgt
     8641 accggcaatt gctccaatcc attcactatt tccaataaac atatccgcaa gtgcattatt
     8701 gacaagaatt cctaaaacaa aaccaactaa gactattaaa acagatccaa tcaaccgaat
     8761 aatcctgatc atctttcatc cctcctttat attaagttta acaacccttt tctttcttgt
     8821 ctcgtgaaaa ctatgtaaat gttctccatc atagagagaa tagattttta cgaactaaga
     8881 atatctacag caaccttacg aaaatgaaag actgtctccc taattcttct gttagactgt
     8941 ttttaataag gaaaggatgg gaaatttttt gaaaaaacaa ctactaatta tgtgcctttc
     9001 agcatgtgca attttactat tctcaggttg tagcaagtcc aatgaaacga ttgcaacgtt
     9061 taaagatgga acacttacag ttcaggattt ttatgataac ccaaaagtta aatcaaataa
     9121 tcaaacacta ttaaaaaaaa tgattgtctt caaagcattt gatgatgtat acggaaaaga
     9181 gatttcttca aaggaagtaa caaaagaata taatgaacaa atcaaaaaac tcggtccaaa
     9241 ctacaaggaa caattaaaag cagttggcca aacagaagag acatataaga aactctttaa
     9301 acagatgctg gctttccaat acggtttgaa agccaatgta aaattaacag ataaagatct
     9361 agacactgct tggaaagaat tctatccaga agttagtaca cagatcattc tattttcgac
     9421 taaagaagaa gcagataaag caaaaaaaga agcaaatgaa ggagaaaatt tctccaagct
     9481 tgttcaagct tatggaaaaa ataaaacatt aaaggaaact gatggaaaaa tgaattttga
     9541 ttcaaccaat ccagagattc ctactgaagt aaaaaaagca gcatttaaac taaaaaacgg
     9601 ggaagtttct gatatcattc ctgtaacaga tccaacgact tatcagcaaa gctattatct
     9661 agtaaaaatg gtaaaaaaac aggataaagg ttctaataaa gacaaataca aatctgagct
     9721 agaaaaaatt gctaccgaag caaaaactct tgatacggaa ttcatggata aaacaatccg
     9781 taaagtgatg aaaaaagaca atgtaactat taaagatcct tatgtaaaaa atatattcaa
     9841 ataaacaaat ttgacccaat tggggatgtg gcaaaatttg agaagtgatt tttggaaatt
     9901 ccttcttatt tttgaggcaa accacttttg ccacaacttc atcggggcct ttttttattt
     9961 gtaccaaaac aaatcactgg atttgttaca atcatgagga tataatttaa aaaagttttt
    10021 tatttctatc acatgaatca atgcgccatt aagtagattc tttcatgctt ccctattttt
    10081 tcacttgccg tatcgcttca aaaacaacta tcataaactc aaactcgact gaaaagttat
    10141 tttaaaaaag actaaaaata tcccttctgg acttgttctt cttattctgt tatactagag
    10201 aaacacgaaa gatattttta tgcgacactt ttcatttgtt tttatgaaat ttattcaaaa
    10261 ctggcgtaac ttcagagtta caaggatgta ttagaggtta gggaatacat cgtttaaatg
    10321 agaaataagt aatagttagt aatagtcacc caaactttaa attaatgaat attgaatcgt
    10381 cagatagcct cctcgagttt tcgagaaggc tatcgtttat tttatggaga aattgattca
    10441 gaagtaatca gcgacctttt ttagttcacc aatcgtttct gcttggaaag tttgcaccca
    10501 atcttcttgg aagcccctca ctgctttatc aatcgagggc atctctaata cttgatctag
    10561 tagctcttta cctaacgtta tcgcatgagc tccagatcga atggctgtag tgacttgttc
    10621 acaatttttg aaactagctg ctaatatttt tgttggtctt tgcgtacgtt cgatctcagt
    10681 ccgcaacgaa tcaatcactt tcatagcatc aatgccttga ttttgcatcc tgttataata
    10741 aggtgcaata taatctgctc cattattaat agctaaatac ccttgtaatt ctgtgtaaat
    10801 agctgtaccg gtaatcaaat aaccttcttt tttcaacttt ttgatcgctt gaaggcctgc
    10861 ttgattcacc ggcaccttga taaacgtatc ttttcccagt tcttctatga taactcgagc
    10921 atcctctatc atttcttctt ccgttcttcc tattgtctga atatggatag gaatctgtcc
    10981 gattattttt ttgatttctt tcatatgcga gaagaagttt gacgctcctt cttttttcag
    11041 aattgtagga tttgttgtta cgcctgttaa attcaaaaac tgttgacaag aataaatact
    11101 ttttgtattt gctgtatcaa gcatgatttc catatagctt tcccccattc tgatcctctt
    11161 gatcgatttt aatgattgtc ccctttctta aatgcggcat ttccttatct tctacataca
    11221 ttgttcctgg aagttccggc tcatgagatc catcaaattt tatcgtgata tgtcctaatt
    11281 cattcaaatt agtttggaca acatctccta ctgctgtgat tacatacatt tgtttatcaa
    11341 aacagagctt catatcacaa gttatttctt ttcttacctg attcaattca atcttataac
    11401 aataatctgt taatgtttcc ggtgcttcat ctccaaataa aatcattatt ttttctttcc
    11461 tgaatacttc tgcttctata ccaacatctg ttacaaccgt ttcaaagatc gtcatctctg
    11521 ttcctcctcg taaaatatca tttgtctaat agtttttatt caaaatgttt ctagattata
    11581 aaatttattg aataacacat tcctaacgga atttgtttcg atattgtgtg atttctcaga
    11641 gtacaatgga tgtatgga
//
LOCUS       DAZKCZ010000079        11653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000079 DAZKCZ010000000
VERSION     DAZKCZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.080"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(595..2226)
                     /locus_tag="ACG8KN_001947"
     CDS             complement(595..2226)
                     /locus_tag="ACG8KN_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFP9188995.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2240..3913)
                     /locus_tag="ACG8KN_001948"
     CDS             complement(2240..3913)
                     /locus_tag="ACG8KN_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFP9188996.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(3926..4243)
                     /locus_tag="ACG8KN_001949"
     CDS             complement(3926..4243)
                     /locus_tag="ACG8KN_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9188997.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4268..5668)
                     /gene="lacG"
                     /locus_tag="ACG8KN_001950"
     CDS             complement(4268..5668)
                     /gene="lacG"
                     /locus_tag="ACG8KN_001950"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFP9188998.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5905..6690
                     /locus_tag="ACG8KN_001951"
     CDS             5905..6690
                     /locus_tag="ACG8KN_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9188999.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6693..7385
                     /locus_tag="ACG8KN_001952"
     CDS             6693..7385
                     /locus_tag="ACG8KN_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9189000.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7659..8030)
                     /locus_tag="ACG8KN_001953"
     CDS             complement(7659..8030)
                     /locus_tag="ACG8KN_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFP9189001.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(8430..8660)
                     /locus_tag="ACG8KN_001954"
     CDS             complement(8430..8660)
                     /locus_tag="ACG8KN_001954"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFP9189002.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(8754..9092)
                     /locus_tag="ACG8KN_001955"
     CDS             complement(8754..9092)
                     /locus_tag="ACG8KN_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189003.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(10020..10136)
                     /locus_tag="ACG8KN_001956"
     CDS             complement(10020..10136)
                     /locus_tag="ACG8KN_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP9189004.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(10612..11490)
                     /locus_tag="ACG8KN_001957"
     CDS             complement(10612..11490)
                     /locus_tag="ACG8KN_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFP9189005.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3624 a         2429 c         1908 g         3692 t
ORIGIN      
        1 tatttttatt tatagtgttt tactgttctg tatattgttt taactccctt gatagtatcg
       61 cattctgttt tatacacttt acgttgaaaa cagagtggga ataaagagcc aacataatat
      121 ggagggggaa tccctataaa tcaacattct tcaatagcta aaatgcttga ttttcattaa
      181 aaataagatg gttccctacc aaaagagact ataatataag ttaatttgtt tcattcttat
      241 cataaaaaga agttctcgaa aaacgaactg ttttccgaga acttttttct atccatctaa
      301 gtgctcaaat catattcacg acggaattct aaggttaccc tttcttaaac aaactttatg
      361 ttatctgcac aactttttgc aattttttca ttgcttctta cgaaatcgtt gaactctctt
      421 tagcataata caaagtgtag atgaagtctg gtgctttact cgttttggta aaaatgactg
      481 gaaatcgttt ctggttattt ttggcaaagg cgaggtgcag gttcgattcc tgccttctac
      541 gctaacgatg tggctgaagg gaacctccga tcttgaatcg aaacgatccg cagtctattg
      601 tttcaccaat gaaacaaaat caacgttcgt ccaagcattt cctgaagcct tgctataaac
      661 cccaatttca caaacacctg ttgtaattgg aatgtctttg attgtaattt ttgtccattc
      721 acttgtttta acaaccgcag tatttagttc cgctccacca taatttttga tatataactg
      781 attagtatca aactctcccc cagacattac ataggcactc aatgtgtaag ttcctttgct
      841 tatgccacta acattttgat aagctgatgc ctcgtatgct tgatcctgcc aaaaagttag
      901 tttgtagtcc ccaaaaaaag tgcccgcttc cgttttgatc gtacccttag cggttccttg
      961 ttgaaaccag gtttgccaat tggaaggact ggtcgttggt ccatcgcttt cgaaacttgc
     1021 atttttaact agatttattt gcgcccctgc ttgtttaaac gcacgcaagc ttggcaaggc
     1081 gtttccagca aaatcaaaca tcgcttggtt gtcccattcg tttcccatag tggattgaac
     1141 actgagataa ttcatacctg cttgtgttgc ccaactcaca tttccgttgt accaaagagg
     1201 ttcccaatag aagaagcccg ccccgcgatt attcggtact gcctgaatcc gatcggcaag
     1261 atccagcaaa tactctgctt gtcctgtcgg tgttggttca tatccccccg cagctgcttc
     1321 ggcttcacca aaagcatttg gcatagtatc ggcattcgct gttgtatgcc cataagctgt
     1381 ttcaactacg atcacttctt taccataccg ttggctgatc gcattcatat tggtttgaag
     1441 ttcattcatt gaaccgtgcc aatacggata atacgacact ccaattaaat caaatgacac
     1501 acctcgactc gtgatttcat caaaccacca agtaaatgct cctgtatcgc caccatcagc
     1561 taagtggagc attactggag ttgttttagg ttgagtgtct cgaacagctt ggataccaga
     1621 cttcaaaaaa gcagccaatc gatcaaattc tcccccgcct tctccccaag acttgccata
     1681 aggccagagc attccgctat tcagttcatt gccgatctga accatatcag gataaacgtc
     1741 cagctgcttc atttccgaca gaacgttctt tgtatagtcg tacaatgccg tattcatttc
     1801 ttcaaaggtc agaccttgcc atgctttagg aagattctgt ttcccaggat caacccaaaa
     1861 atcactgtag tgaaaatcca gcagcacctt aagtcccaga ttttttgctc gttttgcaag
     1921 gtcaatcgtc gtttccagat catttgtccc tgctccataa ctttctccat tactattata
     1981 aggatcctgc cacaaacgca gtcgaacata gttgacacca ttatttttta aaatctctaa
     2041 ggcatctttt gaaatatcgt cttcataata ttttgcacca gatttttcca tatctgacag
     2101 gatcgaaata tctgctccgc gaataaattc gctggcagat gcttcaacag tttttgaaac
     2161 agcccccaaa gcccctaaca cagctgcaaa taaaacagct ttacgcaacc aaattcccat
     2221 tttcattatg gctcctcctt tattgaaatt ggttcagaat aaactgaaca gctttttcag
     2281 gatctctcgt taatccgata tattctgctc cttttgtcgc aaccagcttg attcctaagc
     2341 gatcggtgtc ttctttgata tcatcatagt aagaattgac ttgcggtgct aagaccacca
     2401 tatcaaaatc ctgcagcatt tcgtaatgag aaccataagc acccgccatt gcactgatcg
     2461 gtgctttcga ttcagatgca ccttttttta aagcattagc taacatcgca cttgtccctg
     2521 cgccagcaca aagtaccaac acattgattt ctttatttgc atcgctgata acttgactcg
     2581 ctgccacagg tacttcctca cgtacatcgg cagaagcacc ttcctcggtt gcgatcacct
     2641 ctgctttttg tgcttctttc tctactaaga cagcatcgta tgctttgcaa aatggtagat
     2701 agatcaggaa gtcaacgacg attagaaccg ctgctagaac aaaagctaag atacccatgc
     2761 ccgttcctag aaccaaaccg atcggtgctg gagttgccca aggcaaaacg tacataaagc
     2821 tgttcatgcc caatacatcg acaaaaaact tgaacaggca aacgtttaca acaggtgtta
     2881 acaaaaatgg aataaaaaaa taagggttca aaatcatggg tgccgcaaat agcaatggtt
     2941 cgttgacagc gaaacttact ggaataaagg aagctttccc aactgccttc aactgcttag
     3001 acttagcaaa gagtaggaag ataaaaggaa caaccaatgt tgccccagtt cctcccatag
     3061 ttcctacaaa attccctaaa cccaccgtca gtactttaga agcttgttca ccggaattga
     3121 acaattgcag attggtttcc acattggcat agataattgc cgcaattgcc ggctccacga
     3181 tcgacggtcc atgaacaccg acaaaccaaa acagcgccat cgctccccag ataatagcga
     3241 ttcctaagta cccgtcagct gctgtaaata gcggctgcag caccgtaatg attgcttctg
     3301 caaatgagat ttggaagatt tgtcgaatgc ctaaatcaat taaaacggcc gcaaatactg
     3361 aaaaagagaa aggaaaaata tctcggaagg tttgcgaaat cgttccagga acttctttag
     3421 gcattttaat ggtaatatct ttcagcacac aaaacttata taaattaact gttaaaaatg
     3481 cagcgacaaa ggaagccagt agtccctttg tccccatata ttccgttgaa aatccacctt
     3541 caatcggaga gacgcttaac aacaggaaac ttaccacaga ggccagcatc acagaggtgg
     3601 tgttgattac tttgtttttc ggtaagcgac gattcatcga atcagccaag cagcgagcag
     3661 tggttgctga aacgagtaac cctacaacac ccatcgaata attatagact ttccatagcc
     3721 atgtggagac agattcaggc cattggtaac caaaaatctc aggaaccgca gcaatcaaaa
     3781 tgaaaatgct tgaaaataaa atagctggca tcgctgttaa aaatccgtca cgaacggctc
     3841 ctaaataact attacgtgag attttttcaa aaaatggttt ccctttttcg atttgtttaa
     3901 tgattccatc catagcgttc ttctcctatc cttgattctg tctgtacagt acaataaagt
     3961 cttgaatcag atctctcagc aataaagtgg tcattaaatg atcctgccca tggataaaaa
     4021 tgaatcccat ctccatctct ttcccagatg cttcttccgc taaaattttt gtttgcgaat
     4081 tatgtgcaag ggttaaattt tcgtcggctt ctttcaacgc cgaatcaaca tgttcaaagt
     4141 ttccttgtct tacttctttc aataatttca ataatgtact gcgagcgtcg cctgaatatg
     4201 caacgatctc aaaaccaagc atttgtaatt cctctttatt catcttttct tcctccatta
     4261 tcacttatta aataatcgtt ttggtttcac tgaccaactt gtaccagtaa gctgattctt
     4321 ttgggtaacg cttctgggtt tcaaaatcaa cataaaacaa gccatacctt ttgttatacc
     4381 catttgtcca tgagaaaaga tccataagcg accacaaaaa ataccccttc acatttaccc
     4441 cggctgttat cgccttgctt aaggattcca aatagactct caaatagtcg attctcggct
     4501 gatccataat aatgccatct tcaaactgat ctttataccc catcccgttc tcagtgatat
     4561 agattttgtt gtaatgtggg taatcgcttt taattcgcag caacaagtca tatagacctt
     4621 ctggataaat cagccagtcc caatcagttc gaggaattcc ttctttataa atacgttctc
     4681 cgattccctt cactttataa acagaggtcc ctttttcacc cgtaccgtta tgatggattg
     4741 cattttctcc atcgtaagct ttgacaaaat ggcattggta gtgattgatc cctaagtagt
     4801 cattacgcgt ggatgctttt ttcaattcaa cgaaatcttc ttcaggaaaa tgatagctgg
     4861 cctgatttgc ttcacatatc tcatcaagag cagtcaaggt ttcagttgaa taatagccta
     4921 aataggtagc atctaataag aaacggatcg acaatgcatc gtctaaaaag gcagcatgct
     4981 tatcttccgg cgcgtctgtt gccgcatatt tcgtctctaa tgagtgaact acaccaattt
     5041 cacccggaag ttcgttttct ttgaaatagt tcactacacg ggcatgagca accatcatat
     5101 tatgaagaca ggcgacgatc tttgtaaaat catatttgat tcctggtggg aaaacaccta
     5161 acaaatattg attggttgca actggataga tttcattaaa ggtactccac accttgactt
     5221 ctttgaattc atgaaaacaa aaaatggcat aggaaacaaa tgcttctatt gtttctcgat
     5281 tcaaaaaatc tccatggtca aacaaacgtt taggggtatc aaaatgatgc aacgtcacaa
     5341 acggcgttac gtttcttttc ttacattctt cgaacacacg atgataaaaa gcgattcctt
     5401 ctggatttgg tttcccagct ccatcaggaa aaatgcggct ccaagcaatg gataatctta
     5461 gaccatttac accaaaacgt tcacacaatt caatgtcctt tgcatattga tgataaaaat
     5521 cacttgccgg atcgggactg aatcgccctt gttcctctaa aaagtcatcc caagcaacag
     5581 cgcctttgcc accttcctta gtggctcctt caacctgata ggctgctgtg gctccgccaa
     5641 aaataaaatc ttctggtaat ttaagcatgt acatctctcc tcaaatctat aatcagtttt
     5701 cgctctttct aactgattgc attgtaaacg catccaaaac atttgtcaac aaaaacaaac
     5761 acaaataatc ataaaaacca gaaaacagca ataaaatcta ttgtaacaaa cataaaaacc
     5821 accctaaatg tttacttttg ttcgaattag attgactgtt gtccgtttat atgatagatt
     5881 atccttaaat tagaggtgaa gacaatgttg aaaaaagaaa gattgcttac tatcgtagaa
     5941 atggtcaata aaaaaggaat ccttaccgtt aatgaaatca tcaatcaatt agatgtttct
     6001 gatatgaccg ttcgcagaga tttagatgaa ttagaaaaat cgggcaagct gctgcgtgtc
     6061 catggagggg ctcaaagtct ttcctacact ttagatcaag aactttctca cactgaaaaa
     6121 gccactctgc agattcaaga aaaaaaagaa attgctgcca cagcagcctc cttgattcac
     6181 gagggagaaa cgattttctt aggaccaggt acaacaatcg aactattggc gacgcattta
     6241 ctgaacaaga aaattcggat cattacaaat aattatccag tttttgatat tcttgctcaa
     6301 gcagatggac cagaaattct actgattggc ggtgatttcc ggaaaaatac cggcgcattt
     6361 gtcggaccga tcaccaacga caatttaaga agactaaagt ttacaaaagc atttatcagt
     6421 tcaaatggcg ttcacaacga agcgatctct acttacagta ccgaagaagg agaagcacag
     6481 cagatcgctc taaataattc ccgtaagaaa tatctactcg ttgataataa aaaattcaat
     6541 cgagatgatt tttatgtgtt ttataatttg catgatttcg atctgctcat tacggatcgc
     6601 cagatctcgc gggaggttca agagcactat gcacaatatg tcgatctgca aattgctgat
     6661 tcaaccgaaa ataccactat aggagcgtaa ctatgaaagg gattatattt gatttcaacg
     6721 gaacaatgtt tttggactct catctgcatg aagctgcttg gctgcatatg atacatgatc
     6781 atacaaaaga tggtttatca gacgaggata ttttaaaaaa tattcatggt cggacgaata
     6841 cggagatttt gaaacatttc atttcagagc atttgaccgc ctctgagatc gccgtattgt
     6901 cagaagaaaa agaaagctat tatcgtatgc tttgcttgca aaatgaaaac gagcttcagt
     6961 tgacaaaagg gcttgaagcg actcttgacc agctgactga ccaacagatt ccattaacga
     7021 ttgccacggc tactgtcaaa gagaatgttg ctttttattt tgatattttc gatttagccc
     7081 gctggtttga ctgggacaag attgtctatg acgacggctc tttccctggg aaaccgcaac
     7141 cggatatttt tcttaaagcg gcagacaaac tgagcttagc tccggctgat tgtcttgtta
     7201 tcgaagatgc ttactcagga ttacttgctg ccaaacgtgc ggggatcgga acaattattg
     7261 caattgatcc attcggtaaa aacaaaacag tatttgaaca ggcacagcta aataaagatg
     7321 gaaccatcaa agattttact ggtttttgga cagagcacct gtctgccaca tccgttaatt
     7381 cttaaacgat tcaactaact accaaagaaa agcaactgaa atgcacacct ccttttataa
     7441 aaaactgcca gtgaaacgac atgaaagata cactgctata tccttagtga aatatagcag
     7501 tgtatctttg actttttcat tcttttaacc atatgacgcg ttcatctcta tctataatta
     7561 acattaaaaa tagtgttttc acattatttc tcaaaccaat cttaaaaatc atcaggagta
     7621 cttttttgat atacttttat taatgctggg cgtaatggtt aaaagatttc cactagttcc
     7681 tctccaccaa gctgagttga aaaatgctta tctgtaaaca acatagacaa agaggaaata
     7741 aacgtttccg tatctttctc tgtcccaagg atattaatta attttcctac taggtaagta
     7801 tcattaaact taagatctgc cttcacatca aaagttttga taaaagcttt tatcatgcgt
     7861 acaattttgt ctttatcgcc ataattgatt tcaccactca tatcaatata gtcactaatc
     7921 cctgaagaaa taatggcaac tgcttcttct ttcgccttat cagataattc atttaatgac
     7981 tgggattgat tgatgacttc ttgagtttta tctgttacgt aatctctcat tatcctatcg
     8041 gcatactccg caaagtaatc ttgaaaaaat tcttctacag ggtaaccaac ttcgttttcc
     8101 caaccaggag aaatttcatt atcagtactt attaaatcta ttaaaaacca acccgttctc
     8161 tgagtgttga ttctataata tttttaattt cctatataaa tcaaaaatat tatagaatca
     8221 ataagctaat taagaaacaa actttagaaa ctctagaaga acttctgaat ttatatagaa
     8281 gtgtgtagct acttactaaa tatacaaaag ttaaaataaa atactctgga tactctttat
     8341 actgcattaa agttagtaat attaacagca aagatatcga aaaaacaata acataatatg
     8401 tgtcaaaatt tttcataata agaactccct tactgttgca tactaacata atccaaaaac
     8461 tcttctactt cagctaaaga cattccactt tctttagtcc aaaaaacagt tttttctatc
     8521 gatccttctc tgtaaactgt aaatgtcgga gtcgagttaa ttgagtagcg actcttgaat
     8581 tctttccact cattttcatc ttctctaact tttgctacat tcacgtaata aatattttct
     8641 tttccctttt ctaccagcat atcgaatagc ttcctgcttt tgatgatcga tttcttgtgc
     8701 tacatctagt aaattttcat caatttctat atcataaagt atttcactag tgtctacatc
     8761 cacaatatat actttttatc agtagcaatt tttgagtaat ccgttaaaat gatctcagtc
     8821 tcgtcacttt catcagtata aatatacgtt aacaatgaac tcaccttttc aatcggtgtt
     8881 agctctaagc ctactgtatt ttttactatt gagtcattat ttaccaccat aacttcgtac
     8941 tgtatatacc atcctatttg gtttccatca atatcagtaa ttggctcact agctaatgga
     9001 gtctccggaa caatattttc ctgatatttc ttaaactctt ctgtagcacg aaaaaggtta
     9061 tcatgttcct cacttgaaac tggttgagtc atagaaaatg ttacttggct aaacagaggg
     9121 aaaataaaca ctacaatcaa caaagtataa aaaaaagccc ttctttttca ttttttatct
     9181 tccaatcaca aattgaaaaa cttattcctg caactttgag tctagtatca caatcgactg
     9241 actacaacta ttcatttaca ttctacttta agttcctcct taaccattaa ttttattaaa
     9301 gatacttgta atagtgattc tcacaaaatt tcatgaaaaa tactttatta ctgtatctaa
     9361 aattccaata cacaacttat cgactattat ctgaaaacca aaacttatta ttccttgatg
     9421 ttattctagc aatagtaact gcttttttat ttttttgcat attttttcgg cgttactttt
     9481 taaagttaac tttttcgaca aatagaaaga gatacgaatc actgattttt ttgaagaaaa
     9541 gaaatggagt gagctgaacg ttagagatat tgggagcttt cgtaactgct tattaaacga
     9601 taatggttat ttgcaaccgt attgctcgct tctacatgaa atatttcatg aaatatttcg
     9661 caaaaccttt gattatgtag ttacgtttcc attcttgact aaaaatgttt caaaaacgaa
     9721 caaaggtgtt aaaaggtaaa gtgatcaata ctactggtca aaataagagg ttgaaaggga
     9781 attgaaaaat aactacagat aatactttta taaacgtacg tgctttgtgg ttctgtagtt
     9841 aactatataa ctggaattag ggaatggtat caatttgaaa agaaagattc taccaattta
     9901 tcatatgtta aaaatggaaa atcaacagca gttctttcgg aaaccttaca caaaaaccga
     9961 ttattgaaaa tagccgaaaa acgtttttcg gctatttttt taattaagtt tccttcttct
    10021 taccttttcc cactattccg tttcacatct ttgatgatct tcagcgcacg ttttcgtgtt
    10081 ttgatatttg ggcttttcaa gcggcggtat gcacttgaga tatctgtttt ttccatatgg
    10141 acgacccact ttcttctttg attgtagtca tgctaaaaaa cacaaatcga aacaattacg
    10201 atttgtgttt taaatggtat caaaagttaa aatgaaagtc aacttgttaa aacagtcgat
    10261 cagactagct gattgactcc tatctctgga aataaatttt cattattacg ttttagctac
    10321 ctaaaaaagg ccctcttgtc cgttaacttt aatatctatt gttatagcta gtttacctat
    10381 tgaacgtact gtgtgttcga tagaacacta aaaaagaggc tgggacagaa gcgtttaact
    10441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcagctta
    10501 tttccgaagg agttgcttct gcttccgccg tttatacgtg tttagagcgt gagacaaaag
    10561 tgttctttac ttttgtctca cgctctttta aactatttcg aagtactaaa cttatgaagt
    10621 aagaatacct aagccagagc caatgtgctc gcctgcaagc aattgctctt ttgcttgtcg
    10681 ctgtaaactc gtactttctg gataatcatg tatttgggca tctactcgtc caaagatcca
    10741 agggactcca ctatcgtcct gttcatcaac tgataataat cgataatcgg ctgcaaaaag
    10801 acagattttt gactcttttt tcaaccattt agtaagattg ggtgatagta accaagtatc
    10861 tgtgtacatc acttggtaat ggtaatcagg aaaataaagt tcaaaaaaac gaatggcact
    10921 ttcgtaacta tctcttatct tattctgaga taaatctgca tcggaaggaa tatgaagaga
    10981 aaggcactgc tttccttcta atcttagatc cgtgtaccct ttcggaaaat atgtcatttc
    11041 gtactccaat tcaccaatcc gaaagatcaa gccactagtg taatgccaaa tccaaaaacc
    11101 acggtcaaag cgatagcttc ctgttacttg gaaatgttca tgaataaatc tagtaaaagc
    11161 attcattgtt gccaaataga tagttcttgg aatcttcaat ggttgataaa tttttttcca
    11221 actttgagaa gctgcatata gataaatagc taatgctgca aaaggttcct cttcatagac
    11281 tttgtctatt ttctttgcag tcgcctttgc tttattagga tccattagtt catgactata
    11341 ttgctcaaac acctcatcaa ggatgttttt tgcttgtaca atcatttgct ctataacgac
    11401 tttgggaaga gttaaagttt ctgccagaat ttggatttca ttgcttattt tcttcatcaa
    11461 atttcccctt ttcatatctt tgaacctcat tgtttaaatg actgattatc cggacaccat
    11521 atggtataaa catactttta ttgtacacta actataaaac ttaaactttc tatgaagaaa
    11581 gaatatctgt aagactttca aggaaagcat tgaccatctc aggaaagttt attcagttac
    11641 catcataaac aaa
//
LOCUS       DAZKCZ010000080        11525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000080 DAZKCZ010000000
VERSION     DAZKCZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.081"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..706)
                     /locus_tag="ACG8KN_001958"
     CDS             complement(221..706)
                     /locus_tag="ACG8KN_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFP9189006.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    796..1141
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1186..1827
                     /locus_tag="ACG8KN_001959"
     CDS             1186..1827
                     /locus_tag="ACG8KN_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9189007.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1846..2481
                     /locus_tag="ACG8KN_001960"
     CDS             1846..2481
                     /locus_tag="ACG8KN_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9189008.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2499..3314
                     /locus_tag="ACG8KN_001961"
     CDS             2499..3314
                     /locus_tag="ACG8KN_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9189009.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3633..5069
                     /locus_tag="ACG8KN_001962"
     CDS             3633..5069
                     /locus_tag="ACG8KN_001962"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9189010.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5258..7705
                     /locus_tag="ACG8KN_001963"
     CDS             5258..7705
                     /locus_tag="ACG8KN_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9189011.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7935..9002
                     /gene="rlmN"
                     /locus_tag="ACG8KN_001964"
     CDS             7935..9002
                     /gene="rlmN"
                     /locus_tag="ACG8KN_001964"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP9189012.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8992..9198
                     /locus_tag="ACG8KN_001965"
     CDS             8992..9198
                     /locus_tag="ACG8KN_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189013.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9195..10298)
                     /locus_tag="ACG8KN_001966"
     CDS             complement(9195..10298)
                     /locus_tag="ACG8KN_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9189014.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10851..11399
                     /gene="aac(6')"
                     /locus_tag="ACG8KN_001967"
     CDS             10851..11399
                     /gene="aac(6')"
                     /locus_tag="ACG8KN_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9189015.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3824 a         1948 c         2485 g         3268 t
ORIGIN      
        1 tccgatttat aatcgaaaaa agaagcattt ttatatatgc ttcttttttc gtagaatcaa
       61 taaatatgga tttggctaac tatggaagaa tactcgtatt agttttcagc gttcatacta
      121 gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct aaataaagaa
      181 aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt tcataaactg
      241 gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata atcaacgtag
      301 tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca gcatcgatca
      361 cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg
      421 gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc ttttctttta
      481 attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg acaagataga
      541 tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca ggtagggaaa
      601 aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa gagataccca
      661 gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg catcaccctc
      721 ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc atgtaaatta
      781 cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg tttttagaga
      841 gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt
      901 ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt tgctagatga
      961 ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa
     1021 cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca acgactgtgt
     1081 gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat acgtcctctt
     1141 ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga gtatttgatt
     1201 gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt tttatttacc
     1261 ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt gcattttaag
     1321 ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc gctgttgttg
     1381 caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga tcgtttcgaa
     1441 gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat ctttcgtgga
     1501 gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca attaagtccc
     1561 tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt accttctgta
     1621 gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt aggtttaggt
     1681 gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact gcctttgcta
     1741 ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcgtaat gaaacaagtt
     1801 gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat tgaattgaag
     1861 aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct aatcattcca
     1921 gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt
     1981 atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg tgtaccgttc
     2041 aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca agacttccag
     2101 ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat ggtattgaaa
     2161 cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt agggattagt
     2221 gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg ggtagcgatt
     2281 gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac aagtgcttta
     2341 gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga agatggggta
     2401 acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga tcaattacta
     2461 aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc agttttctat
     2521 tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa acttcagcag
     2581 ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac gatacatttg
     2641 ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt gatttagcca
     2701 aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac tggtcaatga
     2761 aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat tccaaaacgc
     2821 cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat caagtgctga
     2881 tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag agattaggtg
     2941 cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta ttaaaagaga
     3001 tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg atcgatctga
     3061 aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat tatttgaaac
     3121 aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt gaggattttg
     3181 cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa gccctagatc
     3241 aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc gaagatgtta
     3301 cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc cctttttccg
     3361 aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca ggctcacaac
     3421 ctcttggtaa acggtgttca aaagccagtt tttcggtatt aagccaaatt tatgcaaaaa
     3481 tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga ctttttcaca
     3541 acctccttta catattttta ttttgttgtg gaaggggttt cgtgttattc taaaagagga
     3601 acgattgaag gttataggaa aggaagagat gaatggaaca caaagaactt acctcatttc
     3661 caaaagattt tctgtggggc tctgcttctg cagcgtatca agtagaagga gcttggcaag
     3721 aagatgggaa aggagaatca gtctgggatc gatttgtacg gatacctgga aagacattta
     3781 aaggaacgaa cggtgatttg gcagtagacc actatcatcg ttataaagaa gatattgctt
     3841 taatgaaaga acaaggtctg aaagcttacc gcttctctgt agcttggaca cggatttttc
     3901 ctaatggacg aggagaagtc aatcaagctg ggctcgcttt ttatgagaga ttgatcgacg
     3961 aattgattga aaacgatatt gaacctatgt tgactttata tcattgggat cttccacaag
     4021 cattgcaaga tgaatataat ggttgggaat cacgtcagat cattgaagat ttcactcatt
     4081 atgcagaaac attatttgag gcattccgcg ggaaagtcca ttactggatt agtttgaatg
     4141 aacaaaatat cttcacttca ttaggttatt tgttagcagc acatccgcca ggtgtgacag
     4201 acccgaaacg aatgtatgaa gttaaccata ttgctaattt agcaaatgca tctgtcatca
     4261 ataaatttca cgagttagaa atgcctggaa aaatcggacc aagctttgcg tatacgccaa
     4321 attatccaat tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca gaagacctga
     4381 tggccaatta ttggatggat gtctatatgt ggggaaaata tccgattgct gccatgaggt
     4441 tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat
     4501 ctgctaaacc agattttctt gggatcaatt actatcaaac ggcgacgaat gcattcaatc
     4561 cattaaacgg agttggtgct ggaaaaatga atacgacagg aaaaaaaggt tcatcagaag
     4621 aaactggagt tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa cgaacaaatt
     4681 gggactggga gattgaccca gaaggattaa ggatcggact gcgtcgtatc acaagccgtt
     4741 accgcatgcc ggtactgatt acggaaaatg gcttaggaga gtatgacaag ttgacagaag
     4801 ataaacaaat ccatgacgac tatcgaatca attacttaca aagccacgtc aaagccatca
     4861 aagaagcaat ttcggatgga gcagaagtcc taggctattg cacctggtct tatacagatt
     4921 tgcttagctg gctgaacggt tatcaaaagc gatatggatt tgtatatgtt gatcaagacg
     4981 aaacacaaga aggatctttg gaacgatata agaaagacag ttattactgg taccaaaaac
     5041 tgattgaaga aaatggagag aacctttagt tcacatactt aaaacaagaa ggtacggctg
     5101 gatatggccg tgccttttct tgataaaatc atttagaaat taagaaaaac tgtaaaataa
     5161 gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc cttacttttt
     5221 tttgtataat gaaacttgat gaaatgaggg ataataaatg gcacaaaaga aacggcctac
     5281 taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct
     5341 gacgattggt gttgggtttg ccttgatttt atttgctgtc ttcggctttt taaaattagg
     5401 ttttttagga atattgattg caaacggctt tcggatcatt gcaggaaata cttaccaaat
     5461 cctctgtctt ttattagccg tattaggatt ttggattgtc attaagaata cagagttttc
     5521 aattggtaaa aaccgacgct ggtttggcgg catactcttt tatttcggta tcttattatt
     5581 gttacatgcc catttatttg gcaaactcca tactggggaa ccaaatatca tggggacgac
     5641 atgggatttg ttagccagtg atataaaaca aagtcaagta gacaataacg ttggtggtgg
     5701 gatgattgga gcaatccttt atcattttac ttacttcttg attgcacagc ctggaagtta
     5761 tttagcagca gtgctattac tggcaggagg agcattcctg ttcagtaatc tggaaggcta
     5821 tcagctgtta aatggtatcc aatcaattgg agaacgagta caggaattat tggaaggtga
     5881 tcctgccaag caagctcgaa agcaagcggc aaaggaagag agaatgaaac agcgcgcaga
     5941 agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag ctgcagaata
     6001 tgagaagaaa aaagcggtga gccagcaaag ggaaaatact gctgatgaat ggcaggaacc
     6061 agaaaataca gagccggagc agctaagttt tgttccaatc gacagttttc aagaaaatat
     6121 ccatcctgcg aatctagaga aaccagtacc agatacacca aaacagacca atacagcaga
     6181 aggtttttca gatgagttgc cagaggatga cggtacctca ttagaatttg agattgaagc
     6241 agaacaggaa aatcaagact atgaattacc gacagttgat ttgcttgatt ctataccaac
     6301 tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac
     6361 tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac catccgtcac
     6421 aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga acttaacaga
     6481 tgatatcgcg cttgctttag cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa
     6541 atcactgatt ggtattgaag taccaaatgg aaagatcagt atggtttcct ttagagaaat
     6601 catcgaagca caaccaaacc acccagataa attactagaa gtacctttag gacgtgatgt
     6661 ttctggacgg gtacaaacgg ctgatctttc gaagatgcct cacttactcg tggctggttc
     6721 gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc
     6781 taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta
     6841 caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc
     6901 cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt
     6961 aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa
     7021 acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt
     7081 agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg
     7141 gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa
     7201 agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat
     7261 cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat
     7321 gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg
     7381 ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac
     7441 agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc
     7501 aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg
     7561 gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg
     7621 cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac
     7681 gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc
     7741 aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttttcta
     7801 atacggtatg atgtcttgtc tttttagttt ttgtgagatt aaagttctat tcgatttatt
     7861 tgtgaagtat tgcattaagg aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa
     7921 agagggaaaa tactttggaa aaacagtcca tttatggctt gacgaatgaa gaattaatca
     7981 attggtttat agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt
     8041 atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa
     8101 aattatcgga gcattttatc atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg
     8161 atggtacagt aaaatatttg tttgaactcc ctgataaaaa catgattgag actgtactga
     8221 tgagacaaga atatggcttg tcagtttgtg tgacgacaca agtaggatgt aatatcggct
     8281 gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct ggagaaattg
     8341 ttgcacagat catgatggtc caacattatt ttgatgagcg caatcttggc gaacgcgttt
     8401 cacatgtcgt tgtaatgggg atcggtgagc catttgataa ttacgacaat gtaatggact
     8461 ttttgcatat catcaatgat gccaaaggat tggcaattgg tgctcggcac attacagttt
     8521 ccactagtgg attagcacac aaaatcaagg aattcgctaa taacggcttg caagtcaatc
     8581 tggctatttc cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg cggatcaacc
     8641 gaagtttccc aattgagaaa ttgatggaag ctgtggatga gtatttagag aaaacaaacc
     8701 gtcgaatcac gtttgaatat atcatgttga atcaagtaaa tgatagacca gagcatgcgc
     8761 aacaattagc agatctatta aaagataaga aaaaattagc atatgtcaac ttgattccat
     8821 ataatccagt cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat gtattgagat
     8881 tttatgatgt attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa catggtacag
     8941 atattgatgc agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat
     9001 aatcgtatcc tactcgtttt ttcaattacc ataaaaggag cagttttctt aattaaggaa
     9061 ctgttcaact taaataagag aaaagattct atagcgaaag ctataaagct ttttttgaca
     9121 agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga tgacagcctg cttgatgggg
     9181 gttttagcaa ttatttaact gattttctta tatccttgct ggctttcttg tgtagcatga
     9241 taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc ataaggcaca
     9301 tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca
     9361 ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt
     9421 ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca
     9481 gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag
     9541 tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca
     9601 atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc
     9661 aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct
     9721 aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg
     9781 gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct
     9841 gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct
     9901 tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga
     9961 aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg
    10021 ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt
    10081 tcaatatttc cataagttga attacccccg taccagagac tggcgacaac ctctatttcc
    10141 tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc
    10201 agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt
    10261 ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt
    10321 tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag
    10381 gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat
    10441 aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt
    10501 cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa
    10561 ttaagattat tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag
    10621 caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc
    10681 ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga
    10741 taaataatga aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt
    10801 cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca
    10861 gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga
    10921 cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag
    10981 aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc
    11041 ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa
    11101 agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag
    11161 gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg
    11221 acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt
    11281 atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct
    11341 gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat
    11401 aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt
    11461 acttcttgaa tttttgtgaa attccaactt atttccgaag aagctacttt tggaacaccg
    11521 tgtac
//
LOCUS       DAZKCZ010000081        11515 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000081 DAZKCZ010000000
VERSION     DAZKCZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.082"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2154)
                     /gene="glyS"
                     /locus_tag="ACG8KN_001968"
     CDS             complement(79..2154)
                     /gene="glyS"
                     /locus_tag="ACG8KN_001968"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9189016.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="ACG8KN_001969"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="ACG8KN_001969"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9189017.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="ACG8KN_001970"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="ACG8KN_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9189018.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /gene="era"
                     /locus_tag="ACG8KN_001971"
     CDS             complement(4412..5311)
                     /gene="era"
                     /locus_tag="ACG8KN_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9189019.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5721)
                     /locus_tag="ACG8KN_001972"
     CDS             complement(5326..5721)
                     /locus_tag="ACG8KN_001972"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9189020.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="ACG8KN_001973"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="ACG8KN_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9189021.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="ACG8KN_001974"
     CDS             complement(6198..8390)
                     /locus_tag="ACG8KN_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFP9189022.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="ACG8KN_001975"
     CDS             complement(8414..9385)
                     /locus_tag="ACG8KN_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9189023.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="ACG8KN_001976"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="ACG8KN_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9189024.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3112 a         2419 c         2104 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga aaaaa
//
LOCUS       DAZKCZ010000082        10968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000082 DAZKCZ010000000
VERSION     DAZKCZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.083"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..1013
                     /gene="pplA"
                     /locus_tag="ACG8KN_001977"
     CDS             78..1013
                     /gene="pplA"
                     /locus_tag="ACG8KN_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFP9189025.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1103..2206
                     /locus_tag="ACG8KN_001978"
     CDS             1103..2206
                     /locus_tag="ACG8KN_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFP9189026.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2219..3172
                     /gene="menA"
                     /locus_tag="ACG8KN_001979"
     CDS             2219..3172
                     /gene="menA"
                     /locus_tag="ACG8KN_001979"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9189027.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(3986..5332)
                     /gene="gor"
                     /locus_tag="ACG8KN_001980"
     CDS             complement(3986..5332)
                     /gene="gor"
                     /locus_tag="ACG8KN_001980"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9189028.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5466..6719)
                     /locus_tag="ACG8KN_001981"
     CDS             complement(5466..6719)
                     /locus_tag="ACG8KN_001981"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9189029.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(6725..7648)
                     /locus_tag="ACG8KN_001982"
     CDS             complement(6725..7648)
                     /locus_tag="ACG8KN_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9189030.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(7869..10361)
                     /locus_tag="ACG8KN_001983"
     CDS             complement(7869..10361)
                     /locus_tag="ACG8KN_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFP9189031.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10368..10835)
                     /locus_tag="ACG8KN_001984"
     CDS             complement(10368..10835)
                     /locus_tag="ACG8KN_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFP9189032.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         3227 a         2250 c         1926 g         3565 t
ORIGIN      
        1 taggctaaaa agattagaat gtataatgaa tcacaaagaa aaattgtgga ctagaaatgg
       61 aatggtgggg tattaaaatg aagaaaaaac gttttgtaac tggatttgcc gtattagcat
      121 tttcagctct agtgttaggc gcttgcggcg cagatgacaa cggaagctca aactcttctt
      181 ctgaatcatc tacagcacaa tcttcgactg caaaaagcac aacagaatca tctgcaaaag
      241 ttgtagctgg cggagatttg caagacggca catataaatt agaagaaaaa aattattcaa
      301 atggttaccg tgcagtcttt gaaatggttg taaaagacgg caaaatcact gaatcaaaat
      361 atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga tgcagaatac aacaaaaaca
      421 tggaagcaaa atctggcaca aacccagaaa aatacattcc tgagttgaat gaccaattct
      481 taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg agctactcac tcatctgaaa
      541 gcttccaaaa ctacgcacaa caattgatcc aagctgctca agctggtaac acagatacaa
      601 tcgaaatcga caatggtgct gatttgaaag acggcactta caagcttgaa gaaaagaact
      661 actctaacgg ctaccgtgtg caattcgaaa tgacagttgc tggcggcaaa gtaactgaat
      721 caaactttga ttatatcgac aaagacggta aatcaaaaca agatgataca gaatataatg
      781 aaaatatgaa agcaaaaagc ggtacagaac caaaaactta catccctact ctgaacgacg
      841 aattcgtcaa agcaatgggc gaagaagatg gctcacctgc taatgtagaa gttgttacag
      901 gtgctacaca cagctcacat tcattcatca tgtacgcaca acaattagtg aatgcagctg
      961 aaaaaggcga cactcaaaca atcgaagtag ataatatcgt aacagaaaaa taatcacata
     1021 atcatactaa taaaagttgg agggtgggac gatagccaga ttaggcagtc gtctcacctt
     1081 tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta ttttccttaa tcacactggg
     1141 catctctttt atggtgctaa ctggctgcag caactcagca aataaaacaa ccactgaaag
     1201 ttcagcagct accaagatat tgaaagatcc atattctgat gaacagttct tgttagggac
     1261 ttacgttcgt atccgtgtgt atgatgaagg caaagaatca gcattgaaac ctgccttcga
     1321 tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa aaaggttctg agatcgatga
     1381 agtgaatgag caagctggga tcaaaccagt aaaagtttct gatgacgtct acacgttagt
     1441 caagcgggcc tacgaatata gccaagattc tcaaggcgga ttcgatatgg cgattggtgc
     1501 aatcacacaa ttatggcgaa tcggttttga tgacgccaga aaaccttccc aagaagaaat
     1561 cgatcaggca ttgaaattag tggattatca taagatcgaa ctaaatgaca aagaaaaaac
     1621 tgtttatcta aaagaaaaag gaatgatcat cgatttaggt gcaatcgcca aaggttacat
     1681 tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg accactgcca tcgttgactt
     1741 gggcggtaat gtctatgtct taggccacag cccaagaggc gaaaaccaag actggacagt
     1801 cggcatccaa gatccgaaca tggctcgcgg atctgtttta ggttctatta aagaacggaa
     1861 caaaacatta gttacctctg gaatttacga gcgttattta gaagtagatg gcaaaaaata
     1921 ccatcatttg tttgatccaa aaacgggtta tccgttcgac aatgatatcg caagtgtcac
     1981 gatcataaca gataaatcaa ttgatggtga cgggttgtct acagcagtct tttcaatggg
     2041 tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat aacgggacgg aagcgatctt
     2101 cgtgacaaaa gacgataaag tatatgtgac cgatccaatc aaagacacct tcaaattatc
     2161 ggaggactcc cattacacga tgggtgaccg ttctgaacta aaataaggag tacgaaccat
     2221 gacactaaaa atctttttag aagtagttga aatacgaaca aaggtggcta gtgtatttcc
     2281 ttttgcggta ggggtattat tctctatcgc tttttttcat gagatacatt ggctaaacac
     2341 ttttttattt tttattggaa tgctagtttt tgatttagcg actaccgcta tcaataatta
     2401 catggattat gaaaaggctc attctgaagt atataagtat gaagaaaatg tcattggccg
     2461 cagaaatcta tctcctgtat tgattcgaaa catgatttta gctatgattg catttgtttt
     2521 tgttatcggc gttattctaa cgatcaatac gggttggctt ttacttttga tgggatttat
     2581 ctgttgtttt attggagtat tctatacatt cggaccgatt cctttatctc gaatgccttt
     2641 aggggaaatt ttcagtgggg tgactatggg ccttggaatt tttgccatgg tcgtttatgt
     2701 caatacattc ggttcagatg tctatgatct gacagtcaac ttttcatctg gacaatttga
     2761 tttggtagga aatctttggg ctatagcagc aatcgtatta gcttccttac ccttggtgtt
     2821 tacgattgcc aatatcatgt tggctaataa tttgcgggat cttgaccgtg atattgaaaa
     2881 tcatcgctat acactagttt tttatatcgg acgtcctgcg ggaacagtct tattccagct
     2941 attgatgtat gcttgctatt tcgtgatttt aatggggttg ttgagcggta tctatgaatg
     3001 gccgatcttg gtcaccttct tgaccttgcc aattgtgaag aagcatttgg atcaattcaa
     3061 gcaaacgtta ccccagccta tcagtttcag ctattccatc aaaaatatgg tcttattcaa
     3121 tgccagttat gtattggggc tattactaac aatcattatg caagccattt aacagcatga
     3181 agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa cagccaaata aaaatagctc
     3241 cttatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta
     3301 tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg
     3361 ctttccacat ttttgcataa atttggctta ataccgagaa actggctttt gaacaccgtt
     3421 tatcagaggt tgtaaaatca gtgttttgtc ctgagtatta gagaaacaaa tggaaaaata
     3481 gttcctcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacactatt
     3541 tgttcggaaa aagtgactaa ctatgacaca atttttttca cagtcccttt tttcacttga
     3601 tttgattcgt accagcgaac ttgacgagaa ctttcttggt ttggtaacta tatggtattc
     3661 ttttaacatc ctcgtttgct tcttctaatt tatttataca aagtcacact tttttaaaca
     3721 tctgttcttc gatacatttc tatttctttt aaacatcgat accaatattt tgaacttaag
     3781 agtgcatacc aaatggattc atttactctt cgtcagttac gaagataatc aaaatctcca
     3841 acttccctac ttatttcgtt ttgagaatta tcgaagcaat cttacataaa taactaaaaa
     3901 acaaaaaacc agaaaatcag tatactgatt ttctggtttt acatggtttg ttcaataaaa
     3961 agctgttgga actgttaata ttaaattatc gcatcgttac gaattcttcc gctcctgttg
     4021 gatggatggc tactgtattg tcaaagtctt ctttagttgc acccatcttg atagccacgg
     4081 caaaaccttg cagcatctca tcgacaccaa caccaatagc atgtaagccg acgattcttt
     4141 cttcttttcc gacacagatc aatttcattt cgcatttttg gcgataatca tttaatgcaa
     4201 agtacattgg ggtaaagcgt gaacgataaa ctttcaattc attttcgcca tatttttcca
     4261 atgcttcttc ttctgtcaaa ccaattgttg caattggtgg atgcgtaaag acgactgtcg
     4321 gaacaagttt atagtcaaga taagaatctt tctttccatt aaataaacgt tctgataaac
     4381 gtctaccagc tgcgattgca acaggagtca gatctaattt accgatcaca tcaccaacag
     4441 caaaaatatg ttcatccgtt gtattttggt atttatctac tttgataaac ccttttttgt
     4501 ccaattccac atttgtattt tccagaccta atttttcggt attcggtaca cgtccaccgg
     4561 caaaaagtac acaatcacca gttaaggttt caccattttc aaatagcacc gtatattcgt
     4621 cattgttttt agtaatttct ttagcaatat gatgtgcgta agtttttatt ccagcttctt
     4681 ggtacatttg gatcaaattg tccgaaagca ttttatcaaa cgttctaagt ggtcgttctt
     4741 ttcgatatgc ccacatgact tcactaccta aaccacgaaa tacacctgaa agttcagccg
     4801 caatgtatcc agcacctaaa acgattgctc tcttaggtaa ctctgttaat tcaaaaaatc
     4861 catttgaatc aattgcgtat tctccaccag gaatggtcat tttacttgca cggcctccag
     4921 tagcgatcaa gattttagga gcacgatagg tttcaccgtt tacttcgatc gtttgactat
     4981 cgataaaagt agcgtaacct tttatagcct cgacatgatt gctatctaat cctctttgat
     5041 aagcaccgtg tagaaaatca atatattttt ctcgtctttc tactagaccg gcgaaatcaa
     5101 cattttttaa atctgcttgg ataccgtaac ttgaggcatc tcgttcaatt gtttccaaaa
     5161 tcgtacttgc ttgccacata acttttttag gtacacaacc gacgttgaca catgttccgc
     5221 ccaattcatt cgcttcaatc agcaaaactt ttgcgccatg catccctgct ctgtttgctg
     5281 aagcaatacc gccagagccg ccaccaatta cgatgtaatc atatatttgc attttgttcc
     5341 tccttattga agagatcaat caaaaccttt atttaacgat cctttttcta cagaaaacaa
     5401 gtgtaacact tttcttgaat tttgtatgcg aagatgctta aaacagggat atatcagctt
     5461 tttccttatt tacgttttcg gatcatatct ccaggtttca ccggctgtgc gactggcata
     5521 gttaaaatca tcattggatt tggtgctcgg tcaatggatt cgccttcttc attatgcata
     5581 acttcaacgg tttgactgaa atgactgaat cctggaccat aaaattcgat ttcatctcct
     5641 acagaaaaca agtttctctg acgtatcgta gcaatctgtg ttgcctcatc ataagataat
     5701 acttcaccga caaatttata tactgggatt ttacgacgtt caccgaataa ttgttcgttt
     5761 tcagttggtg tattgtagta aaaacctgta gctaattctc gttgtgctac tttccataac
     5821 tcgtcgatcc attcttgctt gcagacatag ttttcaggat cttccatata actgtctaca
     5881 gccgctttat agacatttga caccgtagat acataatgga tcgatttcat tcttccttcg
     5941 atcttgaagc tgtctacgcc gttttcaatc aattctggaa tatgttcgat cattgacata
     6001 tccactgcac tcatcgagaa ttcttcttcc acttcgcctg tatcagttaa actgtttcgt
     6061 tcttgaccaa aaggcatatc gaacaatcca tacttccagc gacaagattg agaacatccg
     6121 ccacggtttg catcccgcat cgacatatgg ttggacaatg tgcatcgacc agaataagag
     6181 atacacatgg caccatggat aaatgcttcg atttcggtat ccgtattttt acgaatctct
     6241 gccacttcct ccattgaaac ttcacgagct aaaacgacac gttccaaccc ttcttctttc
     6301 cagaattcca atgtctcaaa attagttgct gaagcttgag tggataaatg gatcggcaga
     6361 cctggggctt ctgtcgcaca aatttcgatc aaagctggat cagaaacaat cactgcagaa
     6421 ataccgacat cacgcaaact gcggaaaaat tcccctgctc catcttcatt tccttcatgg
     6481 gtgaccatat tagcagcaac gtataccttc gcatcatgtt cttttgcaaa agcaactcct
     6541 tcagccattt cttcataagt aaaattacct gcacggcttc ttaaaccata tgcattcccg
     6601 ccaatatata ctgcatctgc tccataatgg atcgcggttt ttaacttttc cagtgttcct
     6661 gctggcgcta ggatttctgg tcgtttcaat tttcttttta ctgtcatttc tttcatcata
     6721 ctccttattt gatagcagaa ggatcaatat aaaagaatcc tgtatctaat tcacggtttt
     6781 caggatgcaa tttactgatt tcgctagtca ttttttccgc tttttcttga tcccagctac
     6841 cttctaaaat ttgttttttt gcttcggcga atagtcgagt gatgtttacg aatttttcgc
     6901 ctggagtaaa taatccgtcc aatttccatg tacagtaatg atgctgataa agttcattca
     6961 attcattcga gagattcaga tcattgctgg caaaaatatg tgtgccatga ctatcttcat
     7021 aaatagaata atgtgtctct tcttttttag gttccgagat aaaaagacct ctttcacgat
     7081 ctttttgttc gtcttgtttc gtatagttat agtaattctg tagcagtgga cgtttggatt
     7141 gatggatgca tgtagcacca tacacaaggg tttctacagg aatatcgagt ttttcttcca
     7201 tcgccttcat ctcttcaaaa ggaacttcac gagcaagtac tgctccaact gcgccttttt
     7261 tcgcccagaa attgatttga cgtgcgctcg ttactaatgt ttcagcatca taaatatacg
     7321 gtatacgcaa ttcaggattt tttttcatcg tataaataat acctgtatca ccaactgtga
     7381 tcacatctac gtttatttct tttaaaaaag ccaaatattc aggtacattt ttcatttttt
     7441 caggatgaat gatcccattt acagcaatat tcgctttttt accatacttg tgtgctaatt
     7501 ctaccaattg tcgctgttct tctcgtgtaa atgaagcagg cagacgcaat ccaaattgct
     7561 cttcaccgaa atatagtgta tctgcacctg cagcaagcaa ctgttctgct tgttcaatag
     7621 tttctactgt ggttatgagt tcgatcataa ttttctcctc ctacaacttc ataatagtag
     7681 catagctcta aaaactttcc aatacttctt ctatttaaag tcggattttt gaaaattatt
     7741 tcacaaaaaa tcaaaataaa gttcgatata ttctgtccaa atataaaaag agattgtgac
     7801 aaaaatcttg tcacaacctc ttaatcaagt cttactatta taaaaattca gctacacagg
     7861 ccaagtaact atagggaacc tactgtttcc tttttaggtc cgcgaacatt caatgtgatc
     7921 ttaccttttg aagcacctac tgttacgtgg tcacctaaac gaatttctcc gccaagcaaa
     7981 gcctcgctca atcgatcttc aatttctttt tgaagtgcgc gacggattgg acgtgcacca
     8041 tattcaggat caaatccagc tttcccgatc acatcgattg cagatggtgt gattttcact
     8101 tgaatatctt gttctttcaa tcgtttgata atcgctttgc tcatgatttg gacgattgta
     8161 tggatttcat ctttactcaa tgaatggaag acgacagtct catcgattcg attcaagaat
     8221 tcaggtcgga aagctttctt taactcttct aagatacgtt tttgcatggc tttgtgatct
     8281 ttagtaacat cttggacatt gaatccgaca tttttttctt ctcgaatttg tgtcgctccg
     8341 atatttgaag tcataatcat gattgtattt ctaaagtcca cttttctacc tttagaatct
     8401 gttaaatgac catcatccaa tacttgcaga agtaaattga aaacgtctgg atgtgctttc
     8461 tccacttcat ctaagagaat cactgaatat ggtttttgac gaattttttc tgtcaactgt
     8521 ccaccttcat cataccctac gtatcctgga ggagacccga ttaatcggct tgtactgtat
     8581 ttttccataa attctgacat atcaacacgg atcagggcat cttcacttcc aaacatgact
     8641 tctgaaagtg ctttggccaa ttctgttttt ccgacaccag ttggtccaag gaacatgaat
     8701 gaaccaattg gacgatcagg atctttcaat ccgcttcgtg cacggcggat cgcacgtgaa
     8761 actgctttga cagcctcatc ttgaccaacg acacgttcat gcagaagtcc ttccagttct
     8821 aacaagcgtt cgctttcttt tttctccaac tgctgtagag ggacaccagt ccactccgat
     8881 acaactgtag caacatcttc ttctgttaca cgatcagaat aaccttgctc ctgttttgct
     8941 tcaattgcag aaactttttg cagcttttta cgtagtgctt tttcttgtct gcgtaattga
     9001 gcagcatttt caaagtcttg tttttggatc gctgcttcct tttcttgtac tagttcatcg
     9061 atttctagtt tgatcacagt tgattttgtt aagtgatcgg tttgatccaa acggactttc
     9121 gctgctgatt catcgatcaa atcaattgct ttatcgggta gttgacgaga agtgatataa
     9181 cgaacagaaa gattgactgc tgctcgtaca gcttcatctg tgatctccac tccatggtgc
     9241 tcttcatatc ttgtacgtaa tccttgaaga atcacttctg cttcttcagg agttggctca
     9301 tctacttgaa tgcgagcaaa acgtcgctct aatgcagaat ctttttcgat atatttttga
     9361 tattcatcta gggtcgttgc tccgatcgtt tgtaattctc ctcgagctaa cgccggtttc
     9421 aagatattcg aagcatcaat tgctccttct gctccacctg caccaattaa ggtatgcaat
     9481 tcatcaatga ataagattac ttgaccatca ttatagattt cgtcgatgac ctttttcatt
     9541 cgatcttcaa attcaccgcg atatttcgta ccagcaacca aagatcccat atcaagcatc
     9601 atcaggcgct tttcttgcat gtcttctggc acttcaccta aaatgatttt ttgagctaac
     9661 ccttcagcaa tggctgtttt gcctacacca ggttcaccca ccagtacagg gttattcttt
     9721 gtccgtcggc taagaatctg gataagccgt ttcacttctc tcgaacgacc aacaactggg
     9781 tccagcttgt tttcttttgc taatttcgtc aaatcacgtg ctaatgaatc tagcgtaggt
     9841 gttccttgcg gtgtttgccg tttatcttgt acatttacat ttctacgacg attcattcct
     9901 tttccagcag aatcaccagc tagccccatt ttttgtttca gaacttgacg ggcttttgaa
     9961 aggctgatac ctaagttgat catgattcga gtagccatga tactttcatc ttttaataag
    10021 ctcatcaaaa tatgttctgt tccaactttt tgagaaccag ttttcttcac ttcgctgcta
    10081 gctaaagcaa aaacttgttt cattcttggt gaatatggaa gaaagactcc tttagggtaa
    10141 gaacggatac caccataacc aatcagatgt tcaatttctt cataaatttc cgtttcattc
    10201 aaatccattt cacgtaatac ttttccagca attccatttt gctcgaccac taaggctagt
    10261 aaaacatgtt ctgaactaac tgattgatgt ttcaaccgtt ttgcttcttc ttgtgcaatt
    10321 tctaatactg cttgggctct ttcagtaaaa agttcttcca tctaatctca cttcccatct
    10381 tcataactta aacgttccag aaatgctcgg agaatccgag cacgtgtatg atcttcatat
    10441 tcattgaaat tcaatgtgtt ttttgcaatt gcactcaaca tcaagtttcc ttcttttttt
    10501 gtaattattt ggtcttcata aagtttttga ataatagcaa aagcattttt ctcactgatt
    10561 gtatcatcaa ataactgatt tatctgctca agtaattgtt ttttatctga aatccttact
    10621 tttgcaattc gtatataacc accaccacca cgtttgcttt ctactagata accacgttgg
    10681 atggtaaaac gggtattaat cacatagttg atttgtgaag gaacacagtt aaagaggtct
    10741 gccatttcaa tccttcgaat ctcgatcatt tcactatttt ccagaatttt ctttatatat
    10801 gattcaatta aatccgaggt attctgatgt gccatcttat tcattccctt tgactaatat
    10861 tgaccttaat tataacgaat ttgataatag aaagaaagaa gaacgctttt tgttaaataa
    10921 atcctttaga atcaacaaaa aaaaatacgg aatatactat tccgtatc
//
LOCUS       DAZKCZ010000083        10820 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000083 DAZKCZ010000000
VERSION     DAZKCZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.084"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8KN_001985"
     CDS             476..1339
                     /locus_tag="ACG8KN_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9189033.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8KN_001986"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8KN_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9189034.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8KN_001987"
     CDS             3339..3860
                     /locus_tag="ACG8KN_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9189035.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8KN_001988"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8KN_001988"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFP9189036.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8KN_001989"
     CDS             5941..6759
                     /locus_tag="ACG8KN_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFP9189037.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8KN_001990"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8KN_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189038.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8KN_001991"
     CDS             7228..7806
                     /locus_tag="ACG8KN_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9189039.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8KN_001992"
     CDS             7901..8038
                     /locus_tag="ACG8KN_001992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189040.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACG8KN_001993"
     CDS             complement(8851..9354)
                     /locus_tag="ACG8KN_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9189041.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACG8KN_001994"
     CDS             9713..10738
                     /locus_tag="ACG8KN_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9189042.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVHVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT         3306 a         1883 c         2107 g         3524 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gttcatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc
//
LOCUS       DAZKCZ010000084        10574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000084 DAZKCZ010000000
VERSION     DAZKCZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.085"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG8KN_001995"
     tRNA            12..84
                     /locus_tag="ACG8KN_001995"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG8KN_001996"
     tRNA            88..160
                     /locus_tag="ACG8KN_001996"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG8KN_001997"
     tRNA            168..249
                     /locus_tag="ACG8KN_001997"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG8KN_001998"
     tRNA            267..339
                     /locus_tag="ACG8KN_001998"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG8KN_001999"
     tRNA            351..422
                     /locus_tag="ACG8KN_001999"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG8KN_002000"
     tRNA            439..524
                     /locus_tag="ACG8KN_002000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG8KN_002001"
     tRNA            535..608
                     /locus_tag="ACG8KN_002001"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG8KN_002002"
     tRNA            618..691
                     /locus_tag="ACG8KN_002002"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG8KN_002003"
     tRNA            711..783
                     /locus_tag="ACG8KN_002003"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG8KN_002004"
     tRNA            811..884
                     /locus_tag="ACG8KN_002004"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG8KN_002005"
     tRNA            898..971
                     /locus_tag="ACG8KN_002005"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG8KN_002006"
     tRNA            995..1084
                     /locus_tag="ACG8KN_002006"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG8KN_002007"
     tRNA            1097..1170
                     /locus_tag="ACG8KN_002007"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG8KN_002008"
     tRNA            1189..1264
                     /locus_tag="ACG8KN_002008"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG8KN_002009"
     tRNA            1266..1336
                     /locus_tag="ACG8KN_002009"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG8KN_002010"
     tRNA            1350..1423
                     /locus_tag="ACG8KN_002010"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG8KN_002011"
     tRNA            1448..1535
                     /locus_tag="ACG8KN_002011"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG8KN_002012"
     tRNA            1557..1628
                     /locus_tag="ACG8KN_002012"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG8KN_002013"
     tRNA            1651..1740
                     /locus_tag="ACG8KN_002013"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG8KN_002014"
     tRNA            1753..1826
                     /locus_tag="ACG8KN_002014"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG8KN_002015"
     tRNA            1845..1917
                     /locus_tag="ACG8KN_002015"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG8KN_002016"
     tRNA            1921..1993
                     /locus_tag="ACG8KN_002016"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG8KN_002017"
     tRNA            1998..2068
                     /locus_tag="ACG8KN_002017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG8KN_002018"
     tRNA            2081..2154
                     /locus_tag="ACG8KN_002018"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG8KN_002019"
     tRNA            2166..2237
                     /locus_tag="ACG8KN_002019"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG8KN_002020"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG8KN_002020"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9189043.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG8KN_002021"
     CDS             3826..5298
                     /locus_tag="ACG8KN_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9189044.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG8KN_002022"
     CDS             5381..6169
                     /locus_tag="ACG8KN_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9189045.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG8KN_002023"
     CDS             complement(6236..6904)
                     /locus_tag="ACG8KN_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFP9189046.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG8KN_002024"
     CDS             7019..8731
                     /locus_tag="ACG8KN_002024"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9189047.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACG8KN_002025"
     CDS             8734..10506
                     /locus_tag="ACG8KN_002025"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9189048.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3033 a         1926 c         2453 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggca
//
LOCUS       DAZKCZ010000085        10497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000085 DAZKCZ010000000
VERSION     DAZKCZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.086"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..461)
                     /locus_tag="ACG8KN_002026"
                     /pseudo
     CDS             complement(1..461)
                     /locus_tag="ACG8KN_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            complement(713..1072)
                     /gene="rplT"
                     /locus_tag="ACG8KN_002027"
     CDS             complement(713..1072)
                     /gene="rplT"
                     /locus_tag="ACG8KN_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9189049.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="ACG8KN_002028"
     CDS             complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="ACG8KN_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9189050.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1397..1921)
                     /gene="infC"
                     /locus_tag="ACG8KN_002029"
     CDS             complement(1397..1921)
                     /gene="infC"
                     /locus_tag="ACG8KN_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9189051.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1965..2090)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2241..2873
                     /locus_tag="ACG8KN_002030"
     CDS             2241..2873
                     /locus_tag="ACG8KN_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9189052.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2875..3780)
                     /locus_tag="ACG8KN_002031"
     CDS             complement(2875..3780)
                     /locus_tag="ACG8KN_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9189053.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3865..4524)
                     /locus_tag="ACG8KN_002032"
     CDS             complement(3865..4524)
                     /locus_tag="ACG8KN_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9189054.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4840..5784
                     /gene="mvk"
                     /locus_tag="ACG8KN_002033"
     CDS             4840..5784
                     /gene="mvk"
                     /locus_tag="ACG8KN_002033"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFP9189055.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5777..6754
                     /gene="mvaD"
                     /locus_tag="ACG8KN_002034"
     CDS             5777..6754
                     /gene="mvaD"
                     /locus_tag="ACG8KN_002034"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFP9189056.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6767..7852
                     /locus_tag="ACG8KN_002035"
     CDS             6767..7852
                     /locus_tag="ACG8KN_002035"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFP9189057.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7849..8913
                     /gene="fni"
                     /locus_tag="ACG8KN_002036"
     CDS             7849..8913
                     /gene="fni"
                     /locus_tag="ACG8KN_002036"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9189058.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9162..9965)
                     /locus_tag="ACG8KN_002037"
     CDS             complement(9162..9965)
                     /locus_tag="ACG8KN_002037"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP9189059.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9985..10308)
                     /locus_tag="ACG8KN_002038"
                     /pseudo
     CDS             complement(9985..10308)
                     /locus_tag="ACG8KN_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            complement(10341..10478)
                     /locus_tag="ACG8KN_002039"
                     /pseudo
     CDS             complement(10341..10478)
                     /locus_tag="ACG8KN_002039"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3107 a         2220 c         2008 g         3162 t
ORIGIN      
        1 aaatacctaa tgacatcaag atcggcaaca caataactgc aatagaaatc actggtccga
       61 ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc
      121 ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat
      181 ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt
      241 actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca
      301 tcgccgagtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca
      361 tgatgacaaa agccgaccat caagttgtag ccttttacac agtaccagac catgaaaaag
      421 aatgagataa ctaacaaaat accaataata atatcactca tgttattcct ccgtccattt
      481 tgtttttctg ataagcaaac taatatatca gtcaaactca ttctaactta tgtagttagc
      541 gctttcatta aaggatgacc ataaatgttc agttttcaat taaaaaacct taattatgtt
      601 attttagact tatacgttat tgtcctgttt catttgatgg aggattgtaa ataaaaaaag
      661 aggaaagaac ataacttttc gttatcttct ttcctcttta taattgttta gcttatttag
      721 ccaaagcttc ttttgcttgg tcagccaatg cagtaaatgc tgctgcatcg ttaacagcca
      781 agtcagctaa cattttacgg ttgatgtcga tttcagcaac tttcaaaccg tgcattaatt
      841 ttgagtagct caagccattc atacgagctg ctgcgttgat acgagcaatc cacaatttac
      901 ggaagtcgcg tttcttttga cgacgatcgc gatatgcgta gttgtatgaa ttcattactt
      961 gttcttttgc tgttttaaat aaagtgtgtt ttgaaccgta gtaacctttt gctaatttta
     1021 gcactctttt acgacgttta cgagttactg ttccaccttt aacacgtgcc atgtataatt
     1081 cctcctaata aaaataaatc ttcagattct gttatttgta cttcttattt catttttgct
     1141 agttgttggc gaatacgttt gaagtcgcca ctagaaacca tgcttgcttt acgcaattga
     1201 cggcgttgtt tttttgtttt gccgtggaaa cggtgacttg taaacgcgcg gaagcgtttc
     1261 aatccgcctt taccagtacg tttgaaacgt tttgctgatc cgcggtgtgt tttttgtttt
     1321 ggcatgacta tatttcctcc tcaaaatctt tcgttatccg actatcggct gtccaacagc
     1381 cagttagtca ttcaaattac ttactgtcgt ttttcggtgc cagcgtcaag aacatgctgc
     1441 gtccgtccat tttcgctttt tgttccactg ttgcaatatc cgcagtttct tcagctaagc
     1501 gattcaagac tttttgacca atttctttat gggtaatggc acggcctttg aaacggatag
     1561 aagctttcac tttatcgccc ttttccaaga acttacgtgc attacgaagt tttgtattga
     1621 agtcattcaa gtcgatcgta ggacttaagc gaacttcttt tacattgatc actttttgtt
     1681 ttttacgcgc ttcgcggtct ttcttttgtt gttcgaaacg gtatttaccg tagtccatga
     1741 ttcgcgcaac tggtggtttc gcaccagggg caactaacac aagatccaag tttgcttgtt
     1801 ctgcaatatt caatgcttct gcttttgtct taactcctaa ttgctcacca tctgaaccga
     1861 tcaatcgtaa ctctcgtgca cgaatgccgt cgttaaccat catatccttt gctatggtca
     1921 ttcacctcca aatattatga gagaaagaaa agcttaccga ataaacggca ataaaaaaac
     1981 gagttctctt acagaacccg cgcgtacttc acctaataga accagtgaaa actatctagc
     2041 ccagtgacat tatcaactaa ggcgagaagc gggtgcttct gcttttattt ctcaaccatt
     2101 atagtatagc aaaaactaat ctttttgtca atgtttttca tacagtttgt cttccttttc
     2161 tgaaaatgat tagattagca ggctttctag cagtgattcc cgcttctttg ctatactaaa
     2221 gaaaaacgag gtgaacccac atgaaaaaaa tcgaacctat tccaccttct cttcctcttc
     2281 ttacaaaggg agagttttat ttagaagatg aggagatctt ctctggtctt ctgatcaacg
     2341 gaggtaacca aagctatctc gatgccaaaa atatggtatt aagagagagt catatccaaa
     2401 aacttacgat gcaaaaaaca ggtttacgtc attttgaatg cagtaatgtc atttttgaaa
     2461 aatgtgattt ttccaatctg gagtggattg gtggcagttt ccaccaagtc gtgtttcatc
     2521 aatgtaaatt aacaggtacg aattttgccg aaagttatct tcgagactgt actttcactg
     2581 attgtattgc ttcgatggct tcctttagta gtacaaactt gaaagctgtc tccttcgatc
     2641 attgtcaatt agaagacagc gaattttatg aggtcacttg gaaaaacctt tttttgtctg
     2701 acaatcagtt aaccggttcg aactggtttc gaacttctct gaagggatta gactttacga
     2761 ctaatttctt cacaacaatc gctttatctc aagattcttt ggcgggacta atcgtcaatc
     2821 aagagcaagc acttgtgatc gcccaagccc ttggtttgca gatcaaagac tagatcaacg
     2881 aatccgccgc cacagtgcaa taagcatcca aatagataag accaaactga tgatcaaggc
     2941 gatttcccat ccgaaaacgg aatgggtaaa gggtaactgg agattcattc cgaagaatcc
     3001 tgtcacaata gttggaacgg tcaagatcag cgaccagacc gtcaaaaatt tcatcgtgtc
     3061 attcaagttg ttattcagca aattattata agtgccagag agctggtcta aaatctgcga
     3121 agctaagttc gtcatttcaa ctgcttgtcg tgcttcgatc agtgcatcgt ccaactcttc
     3181 tttttccttt tctgacaact tgcgataaat agccagtgcc ttgatttgtt ctaaaagtac
     3241 tacattttgt tttgttcctg taactaagta aaccaaaccg atctctaaat ctgaaagttg
     3301 cagcagattc ttgttcgttg ttttttctct taatttcaga ttaagccgct gacgttcact
     3361 attcacttcc tcgattaaag gaaaaaagga atcagaaatc aaaaacaatg tacgaaacaa
     3421 taaactgtat acactcatgt ctggcatccg ttccaaaagc gatcgaatta gtcgaatcac
     3481 gtactcggta ttcggcgtgg taaatgtaaa caaaccgtct tcttttaaaa taaaagcgat
     3541 aggactagtt tcataatgat tatcgacttt ttgctggtgc ggaacatttg atacaagcag
     3601 taacgcttca tcaaaagaat cgtactctac gcgcgcccgt tcatttttat ctagagaata
     3661 agtcagcatc tcgtcactaa tgccatattc ttcttttaag tttttaacag ctagcacatc
     3721 gttttgctgt acattgatcc aaaaattacg gtcttgatta aaatagtgtt tttcccgcat
     3781 actctatcct cctgacattt gtttccagta taaagaaaaa agtcgctcaa ataaaggttt
     3841 accctttatt tgagcaactg taaatcaaat ggatactttg gtttggcgaa tctctgctaa
     3901 tttttcttcg atcattttca agacttcttc ttttgcttga ggatcttcca caaaattata
     3961 gcggtctcca tcgatttgga tttttggact ttctttgtat tcttcatacc attgatcata
     4021 acgtctgttc aattctttgt aataatcgta tagacttggg tcaaaggaca attgttcata
     4081 cgaacgacca cgtttttcga tacgctctaa catcgtatca aaagatacac gaatatgaac
     4141 taataaatcc ggatgttttt tatgggcagc atagggaagt tcttccatca tgttttccag
     4201 cagttcatca tacacacaaa cttctgtttc tgttgctctt cctagatctg catttagatg
     4261 gaaaagcagt gaatcttcat aaatcgaacg gtccaataca ttattgtcat ctttcattgc
     4321 ttgtttgatg cttgcaaaac gtttattcaa aaaataaatt tgcagcaaaa aagcatactg
     4381 ttcagggttt gcataaaata agggcaagac ttcattatca tcaatcgatt cataaaatgc
     4441 ctgactatca aagtgttcag ccatcatttc tgttaaactt gattttccgg caccaattgt
     4501 acctgctaaa acgatcacgc tcatttacct ccagctcact tcatcactaa atattgtgtt
     4561 taactccaac cttttttaaa tataccccca atatctagta gtgtcaattg tattttttct
     4621 agtcaaagaa gaaaaaaagt caaaaaattc ttaagaaaaa agtgtttttt ctttagaaaa
     4681 cgtattctta gttttacttc tgagatggtt ttagtaaaat aaaggaggaa tgttttttga
     4741 gacccttagt ggacaaaaaa attcgtgaag cagcgcaact cgttttgggc gctttatttt
     4801 ttgcttaaaa atcactttta cgtgagaagg agttagatta tggcaaacta tggccaagga
     4861 gaatcaagcg gaaagatcat attgatgggc gagcacgcgg ttgtttatgg agaaccagcg
     4921 attgcctttc ctttctatgc aacaaaagtc accgcattcc ttgaagagct ggatgcaatg
     4981 gacgatcaac tggtttcttc ctactattca ggaaatttag ccgaagctcc tcatgcatta
     5041 aaaaatatca aaaaattatt cattcactta aaaaaacagc atgacatcca aaaaaacttg
     5101 caactgacca ttgaaagcac gattcctgct gaacgtggaa tgggatcaag cgctgcagtc
     5161 gccacagcag tcactcgtgc tttttatgat tacttagcat ttcctttgtc tcgtgaaata
     5221 ctattagaaa atgtccagct ttcggaaaaa atcgcccacg gtaatcctag tggaatcgat
     5281 gcagccgcta ctagcagctt gcagccgatt tattttacaa aagggcatcc ttttgactac
     5341 ttttctttga acatcgatgc ttttttgatt gtcgctgata caggaatcaa aggacaaaca
     5401 agagaagccg tcaaagatgt cgctcacctc tttgaaaccc agcctcatga aactggacaa
     5461 atgatccaaa aattaggata cttgacgaag caagcaaaac aagcaatcat tgagaattca
     5521 ccagaaacgt tggcacagac aatggatgaa tcacagtcac ttctggaaaa gctgacaatc
     5581 agcaatgatt ttcttaatct attgatccaa acagcaaaag ataccggggc attgggtgcc
     5641 aaattaactg gcggtggacg cggtggctgt atgattgcat tagcacaaac aaaaacaaaa
     5701 gcccaagaaa tcagtcaagc acttgaagat gcgggtgctg ctgaaacttg gatacaagga
     5761 ttaggagtac atacctatgt ttaaaggcaa agcacgcgca tataccaaca ttgctctaat
     5821 caaatattgg ggtaagaaaa atgaagaact catccttcca atgaacaaca gcctttcgtt
     5881 aacattggat gcattttata cagagacaga agtcatcttt tctgatagct atatggtaga
     5941 tgaattttat ctagatggca ccttacaaga cgaaaaagca acaaaaaaag tcagtcagtt
     6001 tcttgacctt tttcgtaaag aagccggcct ctcactaaaa gcttcagtaa tcagtcagaa
     6061 tttcgttcct accgcagctg gattagcctc ctctgccagc gggctagctg ctttagcagg
     6121 agcttgcaat actgctctta agcttggatt agacgatctc tctctttcaa gatttgctcg
     6181 acgcgggtct ggttcagctt gccgaagtat tttcggtggt ttcgttgaat gggaaaaagg
     6241 ccatgacgac ttaagttctt acgctaagcc agtcccttcc gattctttcg aagacgattt
     6301 agcaatggtt ttcgttttga tcaacgacca gaaaaaagaa gtgtccagca gaaatgggat
     6361 gcgtcgaaca gtcgaaacat ccagttttta tcaaggctgg ttagattccg ttgaagggga
     6421 tctatatcaa ttgaaacaag caatcaaaac aaaagatttc caacttctcg gagaaacgat
     6481 ggaaagaaac ggactaaaaa tgcatggaac gactttagct gcccagcctc cattcactta
     6541 ctggtctcct gacagcttaa aagcgatgga tgcggtgcgg caattgagaa aaaaaggcat
     6601 tccatgctac tttacgatgg atgctggccc taatgtcaaa gttttagtgg aaaacagcca
     6661 tttatctgag gtacaagaaa catttaccaa attatttagc aaagaacaag taattactgc
     6721 ccatgctggt ccaggaattg cgattattga ataaagggag atttttatga ttgaagtatc
     6781 tgcaccaggc aaactctata ttgccggaga atatgcagtt gttgaaacag gacatccagc
     6841 agttatcgct gcagtcgatc aattcgtaac agtcactgta gaatccgcac gaaaagtcgg
     6901 aagtatccaa tctgcacaat atagtgggat gcctgtacgt tggacaagac gcaacggaga
     6961 attagtttta gatattcggg aaaatccttt tcattatatc cttgctgcta ttcgtttgac
     7021 tgaaaagtat gcacaagaaa aaaacattct tttatccttt tatgatctga aagtaacaag
     7081 tgagttagac agttcaaatg gccggaaata tggattgggt tctagcggtg ccgtgaccgt
     7141 tgcaacggtc aaagccttga atgtttttta cgcgttgaat ttatctcaat tggagatttt
     7201 caagattgca gcactagcca atttagcagt tcaagataat ggttcctgcg gcgacatcgc
     7261 tgctagctgt tatggtggct ggatcgcttt ctcaaccttc gaccatcctt ggctccaaga
     7321 acaagaaact cagcattcta tcagtgagtt acttgccctg gattggccag gtctatccat
     7381 tgagccattg attgctcctg aagatttacg tttattgatt ggttggacgg gtagccctgc
     7441 ctctacttct gatttggtcg atcaagttca ccgttcgaga gaagacaaaa tggtggctta
     7501 tcagcttttc ttaaaaaaca gtacagaatg tgtcaacgaa atgatcaaag ggtttaaaga
     7561 aaataatgta acgttgatcc aacagatgat tcgaaaaaac cgacaattac tgcatgattt
     7621 atctgcaatc actggggtcg tcatcgaaac gcctgctttg aacaaattgt gtaatttagc
     7681 tgaacagtat gaaggagccg caaaatcttc tggtgcaggt ggtggcgatt gcggaatcgt
     7741 aattgttgac cagaaatctg gcatccttcc tttaatgagt gcatgggaaa aagcagaaat
     7801 cactccactg ccgttacatg tctatagcga tcaaagaaag gaaaaccgat gaatcgaaaa
     7861 gacgaacatg tttcactagc taaagccttt catgataaac aaaaaaacga atttgacttc
     7921 gtacggatca tccataaccc tttgccgcaa atagcagttt ctgatgtcga tctaagtaca
     7981 caggcagttg ggtttacact aagcagccca ttttatatca atgcgatgac tggcggaagc
     8041 gaaaaaacaa aaaaaatcaa ccaagatctt gcaatcgtcg caagagaagc agatttgatg
     8101 attgccactg gttcagtcag cgctgccttg aaagaccctt ctttagccga tacttatacg
     8161 attatgcgac aagaatatcc tcacgggaaa atcattgcca acatcggtgc agggacttct
     8221 gtcgaaagag cccaagaagc tatccgattg tttcacgcag atgccttaca gattcatttg
     8281 aatgcacctc aagagttggt tatgcctgag ggagaccgtg attttaccaa ttggaaagta
     8341 cttattcaag agactcaaac agccattgac gtccccctca tcgtaaaaga agtcggtttt
     8401 ggcatgacaa gagaaacgct caatgatcta gctgctttag gagttcatac agttgatatc
     8461 agcggtcgaa gcggaacaag cttcacccag atcgaaaacg cccgtcgctc caagcgggaa
     8521 ctgagttatt tagctgactg ggggcaatcg acagtctctt cattgcttga agcaaatgaa
     8581 gcagacactt ctatggaaat cctagcctcc ggaggtatcc gcaatgccta tgacatattt
     8641 aaagcccttt gtctcggcgc aaatgcagtg ggtacttcag gaaccgtact gacgcatctg
     8701 atgaatcacg gcgtagaaga aactattata ctgatgaagc aatggcaaga agagcttcgt
     8761 ctgctttata ctatggtcgg cgcaacgaat accgcggccc tacaccaaca atcactgatc
     8821 ttttccggac cagtgaagga ttggtgcgaa gcaagaggga tcgacttagt gaagtatgga
     8881 agacgcacag aaaagagcgt gggacaaaag taaagaacac ttttatccca cgctctaaac
     8941 acgtataaac ggcggaagca aaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     9001 ttgaaaagca attttcgtga attccttctt atttcttgga gttaaacgct tctgtcccag
     9061 cctctttttt cgaataaatg atgtttaatc agctgtcctt cggtattcgt tcaagccgct
     9121 gatttcacaa cctctccact aaacggtgta ctaaagctct tttaaggttg ttttccgata
     9181 taggcaagta tgcctccgtc cacatacaag acatggccgt taacgaaatc agatgcgcta
     9241 gaagccaaga agacagatgg tccagccaaa tcgattggct cgccccagcg tgctgctggc
     9301 gtacgtccaa cgataaattg atcgaatgga tgacgttcac cgttttcttg tgtttcgcgt
     9361 aatggagcag tttgcggtgt agcaatataa cctggtccaa ttccattaca ttggatattg
     9421 tattgaccgt attcagaagc gatgtttttc gtcagcattt ttaatccgcc ttttgccgca
     9481 gcataagcac tgaccgtttc gcgtcctaat tcactcatca tggagcagat attgatgatt
     9541 ttaccgccgc ctttttcgat catatcccga ataactgctt ttgaaacgat gaatggggca
     9601 tttaaatcca cgtcgattac ttggcggaag tctgccgcat ccatttcgat catcggtgtc
     9661 cgtttgatga tccctgcatt gttgactaga atatcaactg aacctacatc ttctttgatc
     9721 tgttgaacca tcgcttgaac cgcttcttca tcggtcacat cgcagacata accttttgca
     9781 tcgatgcctg cttctttata atgcgctata ccttcatcaa ctgattcttg attcaagtta
     9841 ttaaatacga tttttgctcc tgctgtagct aaagagcgtg caatctcaaa tccaatacca
     9901 tagacagcac cggtgactaa tgctactttt ccatctaaac gaaacatatc catactaaat
     9961 tccatcattt tctctccctt atgcttattt taattgatcc atttgcacca tatccatgtc
    10021 tgtataggtg atattttctc cgcacattgc ccagatgaat gagtaattgc ttgtacctac
    10081 acctgaatgg atcgaccagc ttggcgaaat aatggcttgt tcattgtcta cgactaaatg
    10141 ttttgtttca tctggtttgc ccatcatatg gaacacacgt gtatcttcat tggcataatc
    10201 aaaatagaca taagcttcca tacgacgttc atgtgtatga caaggcatcg tattccaagc
    10261 acttcctggt tctaaaatcg tataacccat ttataaatga cagctatcgc tgaagcttct
    10321 actgctgtaa atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta
    10381 ggaatcgcct cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg
    10441 tatttattct ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaaa
//
LOCUS       DAZKCZ010000086        10342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000086 DAZKCZ010000000
VERSION     DAZKCZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.087"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..560
                     /locus_tag="ACG8KN_002040"
     CDS             <1..560
                     /locus_tag="ACG8KN_002040"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9189060.1"
                     /translation="IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYK
                     FRSMCLDAEEKLEELLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKG
                     DMSLIGPRPPLEREVAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIS
                     HRSLMYDLRLILKTFYVIVKPNGAY"
     gene            1787..2467
                     /locus_tag="ACG8KN_002041"
     CDS             1787..2467
                     /locus_tag="ACG8KN_002041"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9189061.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            2496..3809
                     /locus_tag="ACG8KN_002042"
     CDS             2496..3809
                     /locus_tag="ACG8KN_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HFP9189062.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            4639..5382
                     /locus_tag="ACG8KN_002043"
     CDS             4639..5382
                     /locus_tag="ACG8KN_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HFP9189063.1"
                     /translation="MSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVVTPNVDHIV
                     QLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLCKMAAEKGY
                     TMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDSQIKRANPD
                     MLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSDYGFEWLFR
                     ITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            5409..6410
                     /locus_tag="ACG8KN_002044"
     CDS             5409..6410
                     /locus_tag="ACG8KN_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFP9189064.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            6433..7719
                     /locus_tag="ACG8KN_002045"
     CDS             6433..7719
                     /locus_tag="ACG8KN_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HFP9189065.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            7753..8406
                     /locus_tag="ACG8KN_002046"
     CDS             7753..8406
                     /locus_tag="ACG8KN_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9189066.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            8439..9644
                     /locus_tag="ACG8KN_002047"
     CDS             8439..9644
                     /locus_tag="ACG8KN_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9189067.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            9720..>10342
                     /locus_tag="ACG8KN_002048"
     CDS             9720..>10342
                     /locus_tag="ACG8KN_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189068.1"
                     /translation="MEIRISYYFRKLLLFYVCVIVAAMSYHLTGNSNVASLIAVVGVV
                     YAIVKACNNEYLAIWVMFVLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKV
                     KARSRYGIVYSVMLIFILIACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSE
                     RITKNEFIDLVCKFTRWTMCIYWFEWILVNMGFNITFLQTGARWGTR"
BASE COUNT         3349 a         1463 c         2417 g         3113 t
ORIGIN      
        1 ttattggttt gattttagct tctcctttga tgttctatgt agcttatcga atcagaaaag
       61 aagagccagg aagtccgata tttttttgcc atacacgtgt tggtaaaaat ggaaaaacat
      121 tcaaaatgta taaattccgt tcgatgtgtt tagatgctga agaaaaattg gaagaattac
      181 ttgatcaaaa tgaaatagaa ggtgcaatgt tcaagatgaa agaagatccc cgtgtcacta
      241 aaataggtaa aattatccgt gccaaaagca ttgatgaatt gccacagcta tggaatgttt
      301 taaaaggcga tatgagtcta attggtccac ggccaccttt ggagagggaa gtagctgagt
      361 acacgagata tgatcttcaa cgcttgtatg taaaacctgg ttgttcaggt ttgtggcaag
      421 ttagtgatag aaataatgtt agtttccata aaatggttga gttggattta gagtatatta
      481 gtcatcgaag tttaatgtac gacttgaggt tgattttaaa aacgttttat gttattgtca
      541 agcccaatgg agcatattag ttagggatag aatgatggat tgttaataaa aaagattaca
      601 catgttcata ttatcgtttc aaaagtaata actgtaatta tggagggttt gaatcacttg
      661 tagaaaatct aaaaaaagaa aggtgaatgg ggcaattcag tatcattatt cttatataaa
      721 agaaaatatt cgaaattttg ggatgatacc actcttaaat ataatgatct aaatgcttta
      781 atattggtat atccgatata ggaactgacg agctatttag tatgatacaa aagtatattg
      841 atagccatta ataaattttc caaaaaataa tgacagcaca ccaattttta taatttagct
      901 tgttgaatcg gtatgttcat gggacggttc aaaaaaagaa tcgaaaaaat aaacggtaaa
      961 attttagtga atcctatgta ataaaatttg gatagatgga aaaacagcac ataaggaggc
     1021 tgagtttgtt gtcatttctg ctcctacaaa ctatggtagt gaatgtgtta ttgaattagt
     1081 gatgtctgtt acttcagaga cattggggat aattaacttc actatacctg tagggtatac
     1141 aaaaaatgga agaaaaaata ttatcaatag attttattta gttcagaatt tttacgtgga
     1201 agtaaattat tgatgataat ctttatccaa ttagaattat tatcgtagga tgtgatgatg
     1261 atagccgtga agttgttcaa gtatttacca atgtctacaa cgcagaaaat tagtttgggt
     1321 ttttcggatt ggaaaatttc aatgtataaa atcataaatt tgctgtaaat agacagtttg
     1381 gggtagtaat agcccaataa attgttcgat aaactcatct ttggggtgag aatagctcaa
     1441 tcattgaatt tcaaacctgc ctttggggtg ggattagagg tcaatatgta cctacataat
     1501 actttttgga aaatgatggt gttgagaatg taagtacatt tcaatgtgaa attctaatat
     1561 agccccaaac gaggtcaata aaaatgacct aaactcgtat ttctccccta aacgaggagg
     1621 agatagatat gaaaattgaa tgtgccggcg ttggagttgg ctgcttttag taggaatgaa
     1681 catggttcga tgagaactgt ttcagataga gtggtcgatg atcacaggcc tttaagtcgt
     1741 ttacagatga tgagacttgt aaaaatagag ggacgaatgg aaaaagatga ttggtataga
     1801 agaaaagaaa ggtgttcaga aagacgtaac aattgatagg gaagagaatc ttaggggaca
     1861 tagattctat tggattggac gcagagcaca agatatcttc ttctcggctc tagctttgat
     1921 tgcattgagc cctgtgatgc taattacagc gctcgccatt gtgattgacg atcccggtgg
     1981 cagcccgatt tttgtgcagg atagagttgg aagaaatgga aagctgttca agttttataa
     2041 attccgatct atgtgtgtag atgctgaagc aaaattagaa gccttgttgg cacaaaacga
     2101 gatggatggt ccggtcttca agattaaaga tgacccaaga atcacgagag ttggtaaatt
     2161 tattcgaaag acaagtatcg atgagttacc tcagctctgg aacattctta aaggtgatat
     2221 gagtattatt ggacccagac ctgctttacc gagagaggtg gaacaatacg gtgactacga
     2281 aaggcagcgg ttgttcgtac agcctggtct tagttgctat tggcagattg ctccacatcg
     2341 taatgaactg tcctttgaag aatgggtgga acttgatgtg aagtacatca atgaaagaag
     2401 ctttatcacg gactggaaga ttatcttctt gactttacag gctgtattat ttggatatgg
     2461 tgaatgatta tatatagtat taggagttga aatatgtgaa tattatttta ttatcgggtg
     2521 gtagcggtag gcggttatgg ccgctgtcca acgatattcg tagcaaacaa ttcatcaaac
     2581 tgtttaagga tgaaaatggc gaatatgaat ctatgattca aagagtttat cgccagatta
     2641 caactgttga cccagatgca aaggtcacta ttgcaacaag taagtctcag gctagtgcaa
     2701 tccataatca gttgggagat aaggcttcga tttgtgtaga accttgtcgt agagatactt
     2761 tccctgcaat tttgttagct gcagcatatc ttcacgatga acttggcgta ggtgatgatg
     2821 aaagtgttgc ggtttgtcct gttgatcctt atgtagataa ttcctattac gaaggcgttg
     2881 aagcccttga gaagttggtg cagcagaata cggctaattt gactttgatg ggtattgcac
     2941 ctacatatcc tagtgaaaag tacggttata tcatccctga aagttctaac gaagtaagta
     3001 ctgttaagga gtttaaggaa aaacctgaca aagcaacggc agagaaatat ttagctcaga
     3061 atgctgtttg gaatgccggt gtatttgcgt tcaaacttgg ttacctgttg gaaaaagctc
     3121 acgcaatggt ggattttgaa gattatcgtg atttgtattc caagtatgag actttaacca
     3181 agatcagctt tgactatgct gtggtcgaaa aagaaacgag cattcaagtg atgcgttata
     3241 acggggattg gaaagatgtc ggaacttgga acatgatggc tgaagttatg gctgataata
     3301 ttaaggggaa agctgtaatg gatgatacct gtgttaatac tcagattgtg aatgagttga
     3361 atattcctat tcttgctatg ggctgcaaag atatggtaat tgcagcaagt ggcgatggaa
     3421 ttctgatttc tgataaagag cgcagtggct atatgaagcc gtatgttgag aaaatcagta
     3481 cggaagtaat gtttgcagaa aaatcctggg gtacatatac cgttatggac gttcaggaag
     3541 gctccatgac tgttaaagtt tctatgcagg ccggacagaa gttgagctac cactaccaca
     3601 attctcgcga tgaagtttgg actgttgtag caggacaggg taaagcgatt gtagatggta
     3661 tgaagcagat tcttcgtgcg ggtgatgtgg tgactattgc cgcaggatgt aaacatacaa
     3721 tcgaagccgt gacaaatctg gatattatcg aagttcagat tggtgaaact attagcgtcg
     3781 ctgacaagta taagagttct ttgaactaaa agttcgcgag gtagttatta tggagatact
     3841 aaaatttcaa cccgtgggta aagattatct ttggggtgga gaacggttta agacggaatt
     3901 tggaaaaaat tgacttaaca ccattaagct tatacgtggg aatgcttagt tcatctagac
     3961 gataactacg tacggaaata tcaaatatga aggacagaat ggtaaaaccg agttgtggta
     4021 tgtattggaa gctacaccgg ggggtgattt atgcttttga gtacgatgta acagaagaaa
     4081 tgttacgaaa tgctgtgatg gacagatcac ttgggaaaca tttatataaa gaagaagtac
     4141 acaagggaga tcttttttat gtcagttcat ggtattggcg ctggagtatt agtggctgaa
     4201 atacaggaaa gctcaaatgt tacctatcga gtttacgatt atgatcgagt agataagaat
     4261 gataataagt gagaattgta ctttgaaaaa gcgattcagg taatgaatat ggaagccact
     4321 ttgaaattgg agaaaagaaa taagctcatt catttttatc ctggtggctc tcgtgaaata
     4381 ctgtgtagat gtaaatactg tgagcccgaa agaatccaag taaagacggg gttttcgttc
     4441 tcggtaaaag aggaatcttt tcaagttctt ttatgtatat cgggggacgg acaagttgag
     4501 tcggcaaatg gtgttatgaa actcattaga tttgtaaaaa agatattgta tgtttttgcc
     4561 aacgaatatt ggaagatgtt aagtgttgag cgatgcggac ttactgaaaa ttagatgggg
     4621 atagagggag gatatctaat gtcacgaatg agatttatga acacagaaat tgacaatctt
     4681 acaatgggtg aagtattggt agaggttgaa aaaatgattc aagataggag atgcggatat
     4741 gttgtaactc cgaatgtgga tcacattgtt cagttggaaa caggaaaaga gttgcaagat
     4801 gtttataaaa acgcatcttt aattttgacg gatggtaaac cattgatttg gatttcaaag
     4861 ttttataaga caccaataaa agaaaaaata tcgggatccg atatattttc tttactgtgc
     4921 aagatggctg cagaaaaggg atacacaatg tattttttag gtgcggcaga gggcgttgcg
     4981 gctaaagcgg cagataacct gatgaaaaga tttccgggat taaatgttgt aggaacatac
     5041 tctcctcctt atgggttcga aaaagacgaa gatgaaatgt ttaaaattga ttcccaaatt
     5101 aaacgagcaa atccagatat gcttattgtg ggtttgggtt gccctaaaca ggaaaaattt
     5161 atgttttata actgtgatag attgggagta cctgtgtctt ttggtctagg agctagtttg
     5221 gactttgagg cagggaatgt taaaagagca ccaaaatgga tgtcagatta tggatttgag
     5281 tggctgttta gaattactca ggaccctaaa agaatggcaa aacgatattt ggttgatgat
     5341 ttaaaaatct ttaaattagc actaaaatat cggaaaaaat aaactatatt caatgaggac
     5401 aatgaagtat gattattaca aaaacacctt ttcgcatgtc tttttttggc ggcggaacgg
     5461 atatggaaga ttattttcga gaaaatggcg gtgcagtgct ttccactact tttgataagt
     5521 actgttatgt gaatgtgcgt catttgccta gattttttga ttacagcaca gagttatcat
     5581 attctcgtac tgagagagta acaaatattg aagatatcga gcatcctgca attcgtaatg
     5641 cgatgaaaat gctagatatg catgaaattc gccttacata tgaagcagat cttcctgcac
     5701 gttctggtct gggtacatct agttcttttg ccgtaggtat gttgaatgct ttctatgctt
     5761 tgaagggcaa gtacgctgac aagaagaaac tcgttgataa agcaatttat cttgaaagag
     5821 tcctttgtaa tgaagcaggc ggatggcaag atcagattgc agcagcttat ggtggcttta
     5881 accgaatcaa tttcaactca gatggctatg aagtattgcc tattattatt acgcctgagc
     5941 gtaaaaagca gcttaacaac aacttgatga tgttctttac tggattcacc agattttcct
     6001 ctgatgttca gaaggcaaat aatgtcagcg gcacagagga caaaagagta agactgaaaa
     6061 aaatgtatga gcttgtggat gaagcagaag cggttctgac tgataagaac agagatctag
     6121 atgatttcgg cagactgctt gatctcactt ggagattgaa aaaggctacc ggtggtgcga
     6181 ttagcactgg aagtatcgat gaactttatg aaaagggcat ggctgctggt gcattgggcg
     6241 gtaaacttct cggagcaggt ggcggcggtt tcttggtttt ttatgttcag cctgaaaaac
     6301 aaaatgctgt gcgttcggca atgagtaatt tgatgtatat tccgtttgaa tttgaaaacg
     6361 gtggaacaca ggttattcat tacacaccgg aaatgtatat tccaaaataa agaaatcagc
     6421 gaggataaga atatgaaagt tgttattatg gcaggcggta agggaactcg catctcatcg
     6481 gttgcaagcg atattccaaa gcccatgatt aaaattgaaa acaaaccggt attggagcac
     6541 gaaatcgaat gtctgagaga ccagggattc acagatatta ttcttaccgt tagccatctt
     6601 ggcgatatca ttatgaacta ttttggcgat ggttctggaa tttcaccagt tactggtcag
     6661 ccctttgacg ttaaaattga atactttttg gagaaagagc cattaggaaa tgccggtgca
     6721 ttgtttcgtt taaaggataa tttgaccgaa gacttcctcc tgctgaatgc agatgcgatg
     6781 tttgacgttg atttcaaccg atttgtagca tatcaccgtg aaaagggtgg cttggtgaca
     6841 ttgtttactc accctaacag tcatccatat gacagcggtc taatcattgc agataaggat
     6901 ggctctgtag acagatggct tgtaaaggaa gattcccgtc caacttatta ccgtaacaga
     6961 gtaaatgcgg gactccatgt gattagtcct gctgttcttg atgtcgagat aaacacacct
     7021 aagattgact tggaccgtca gttattaaag ccgttggcag gaaccggaaa aatgttctgc
     7081 tatgatagtc cagaatacgt aaaagacatg ggtacccctg atcgttatta tgctgtctgt
     7141 gaagatttta aggctggaag agtgaagggc aaaaatcttc agaataagca gaaagctgtt
     7201 ttccttgatc gtgatggaac gattaatcaa tatgttggct ttctgcgtca tattgatgag
     7261 ttcgaactaa tcaagggtac tgaagatgca gtgaaaaaga tcaatgccgc tggatatctg
     7321 gctattgtaa taacgaatca gccagtaatt gcaagaggtg aagtttcttt tgaagaattg
     7381 gaagagatcc ataacaaaat ggagacactt cttggatata agggcgctta tttggatgca
     7441 gtttattatt gcccgcatca tccacataag ggatatgagg aagagcgccc agagctaaaa
     7501 ttcgattgtg aatgcagaaa gccaaagccc ggaatgttaa ttcaggcagc tacggacttc
     7561 aacatagatt tgagtcagtc ttggatgatt ggcgatggcg agaatgatat taaagcgggc
     7621 caagccgccg gctgtaagac tgcattggtt ggcgagagtg agtatggaca ggatatgacc
     7681 atagcaaccc tgagtgaatt tgcagataag atctgctaag ggatgtttta tcacgaaagg
     7741 agagtataaa caatgataga caatagatta cagaaacaca ttgatttgct gattaaacgc
     7801 tatcccactc ttgaagtaat caagcaggat attgttgacg gatacctgat tatggaggaa
     7861 tgctatttaa atgatggcaa gctgctgata gcaggaaatg gcggatctgc tgctgattcc
     7921 gagcatattg cgggtgagct gatgaaaagc tttaagacgc ctcgccctgt tagtagggaa
     7981 tttgcagata aattgattgc ggttgatcct gtaaagggtt ctgaattagc taagaatctt
     8041 gaatatggtt tgatggcgat tcctctcgtt gctcatgaag cgctttccac tgcgtatatt
     8101 aatgatgttg atggcctagg tgttttcgca cagcagttgt ttggatttgg aagacctggc
     8161 gatgtattcc ttggtatttc gacatccggc aatagcaaaa atgtgataaa tgcaactgtt
     8221 gttgctagag ctcttggtat gaaagtaatt ggcttaactg gagccaaagg tggagaactt
     8281 gcaagggtag cagatgtttg tgttaaagct cctgaaacag aaacatatat gattcaggag
     8341 taccatttgc caatttacca ctgttggtgt ctaatgttgg aagataaatt ttttgggaat
     8401 agataaaata tctaagccca tattgaaagg ataaaattat gaatgtacta tttataacaa
     8461 ctcaatttcc atatcctcta gataatggcg gaaaaattgg tgcgtacaac ggtatcagtg
     8521 ttttatctag agactacgat gtgacggtat tgtctttcac cgaagaaccg cagtatgttg
     8581 aagaaggtgt cagttttttt cgagagaaat ttaatagtgt aaaatttgaa aatcctgtga
     8641 tacatgatgt acacataaga aataatccaa tgaaattggt agaagcttta gcaatagggt
     8701 attgtctacg ccaaccttat atagtttcaa agttttatga ttcaaaaatg ctgcagttaa
     8761 tagatgaaaa attttctaac aatgcatatt gggatatcat ttttattgac tatctgaata
     8821 tgcaaagtta tggtcaatac atacgggaga agtataaaac acaatttggt aagtatatct
     8881 taaaagacca caacatagaa tatgatttag tcctacagga agctgaatca agtcgaggtt
     8941 taaaaaagaa gttgcttcag ttagagtata agcgtactcg aaagtatgaa gaggccgcag
     9001 taaatagggc cgattattgc tttagtgtgt gtgatgcaaa tacggaagta ctgaaaaaga
     9061 ttaatgacaa cagttatacg atgcttccaa cttatgaaat gcttacaggg gtgaaaaaaa
     9121 atatagagaa gaacacgatc ctatatttgg gtaacctttc ttggaaatcc aatatgcaag
     9181 gattagtttg gtttttagag tcaatttatc caaatattca acaaagaatt ccggatgtaa
     9241 aaatcacaat tgtcggtagc gggccagttg gaacagattt tagtaaatat tcaggcgtgg
     9301 attatcgtgg gtatgtaaaa gatatttcac acatttatga cgatcaagcg gtgctaattg
     9361 tacctttgtt agaaggtagt ggtataagaa taaaaatatt agaagcgttc aataatgaaa
     9421 ttgcagttgt atctacatta gttgggtgtg atacgattgg cgcaaaagat ggaagtgagt
     9481 tatttatagc agataacgaa aaagattttg caacggctgt agttagactg cttgaagaca
     9541 tgaatttgaa tgaaaaagta agaaagaatg caaaattatt cttaaaagaa aaatactcct
     9601 taacggctag acaagatgag tttagtgcga taattaacag ctaatatata tataagctta
     9661 tctgatgatg atttatagga ggaaatatat ggagatggct gagagaaagg aaaagaaact
     9721 tggaaataag aatatcatat tattttagaa aactcctgtt attttatgtt tgtgtgattg
     9781 ttgcggctat gagttaccac cttacaggca attccaatgt agcgtcatta attgctgtag
     9841 taggagtagt ttatgctatt gtaaaggcgt gtaataacga gtatctggct atatgggtta
     9901 tgtttgtatt aggttctgat atatggggga tagtgccata tattaacatt ggtagtggtg
     9961 tgttgcattg gtcagatttt aatgttatag tagcgatatt tctctgcttg agattcatca
    10021 aagttaaagc taggagtaga tacgggattg tctatagtgt aatgcttata tttattttga
    10081 ttgcatgtgt gcaatcgcat tcaatctatg gacaagatgt acatacaacg ctcttaacag
    10141 ctagagaatt gctagttgta atatcctttt ggccgatagt aaaaatagta tattcagaaa
    10201 gaataactaa aaatgaattc attgatttag tgtgtaaatt cacaagatgg acaatgtgta
    10261 tatattggtt tgaatggatt cttgttaata tgggattcaa tataaccttt ttacagacag
    10321 gtgcacgctg gggtactaga at
//
LOCUS       DAZKCZ010000087        10104 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000087 DAZKCZ010000000
VERSION     DAZKCZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.088"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..233)
                     /locus_tag="ACG8KN_002049"
     CDS             complement(36..233)
                     /locus_tag="ACG8KN_002049"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9189069.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(379..1257)
                     /locus_tag="ACG8KN_002050"
     CDS             complement(379..1257)
                     /locus_tag="ACG8KN_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9189070.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1296..2414)
                     /locus_tag="ACG8KN_002051"
     CDS             complement(1296..2414)
                     /locus_tag="ACG8KN_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFP9189071.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2411..4510)
                     /locus_tag="ACG8KN_002052"
     CDS             complement(2411..4510)
                     /locus_tag="ACG8KN_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFP9189072.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4561..5382)
                     /locus_tag="ACG8KN_002053"
     CDS             complement(4561..5382)
                     /locus_tag="ACG8KN_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9189073.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5379..6188)
                     /locus_tag="ACG8KN_002054"
     CDS             complement(5379..6188)
                     /locus_tag="ACG8KN_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9189074.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6209..6703)
                     /locus_tag="ACG8KN_002055"
     CDS             complement(6209..6703)
                     /locus_tag="ACG8KN_002055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9189075.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6715..7215)
                     /locus_tag="ACG8KN_002056"
     CDS             complement(6715..7215)
                     /locus_tag="ACG8KN_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9189076.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7280..9976)
                     /locus_tag="ACG8KN_002057"
     CDS             complement(7280..9976)
                     /locus_tag="ACG8KN_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9189077.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3072 a         2270 c         1662 g         3100 t
ORIGIN      
        1 aattacgatt tattttatac ggctgaaaaa atctatcacc aaaaaaattc aaacattaag
       61 ccaaggatta tgaagtcaac atttcgaaaa atgaacatac attggtaagc aactataact
      121 attaccacag acataaagta acacaatata ttactattta agtgtacgcc accagtgaat
      181 atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga aacgcagaag catttccttt
      241 cagtgattta atgaaaacaa cacgatttga cttagagcct ttttatttga attcattttc
      301 gctttatcca acttcatttg atcatttgat tactcggtta aatacttttg acatgagtat
      361 caatgaaaca ttcattaatt atggttgctt atttcttacc gcttttctgg ccaatgctaa
      421 tccgccgatt attccggcat tatcacctaa ttcgggcgta ataatgtagc tatcaattgg
      481 tggtatttca acatatccat ttataagctc aacaaattta tcctgaactt ttttcttcat
      541 gtgttcttgt ttcattacac caccgccaaa aataattcta tctggcgaaa acatcagtgt
      601 tgtattgtat gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc
      661 caataaattc tggcctttta cacccaaccg tttttcaatc gctggtcctg ctgccatccc
      721 ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt
      781 gatcaacata tgccccattt ccggatgact gaacccttcc acgaaagtcc cattgaacaa
      841 ggcaccacct ccgatacctg ttcccacagt gaagtatgca acacctgcta accctttgcc
      901 agaaccagca acatattcac catagcaagc cgcattgaca tctgttgtcc attcaatcgg
      961 aatattgaaa tattgcttta gcgttcctac aaaattgaaa ttttgccaag caagttttgg
     1021 tgttgatgtg atatgaccga aactcgttga tttgcattga atatcaattg gaccaaagca
     1081 gccgaccccg atgcctgcca attgttcctt aaaaggttta aaaaaatcaa tgacctgctt
     1141 cattgtttca actggagttg ttgtgggaaa actgactctt tccaaaatcg ttaattcctc
     1201 agtaccgact gcacagacaa atttagtccc tccagcttca attgccccat atatcatcat
     1261 aggttcctcc cttcgaatat gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca
     1321 gtgaaatgac gtttagtatt tcctgaagct tctgttgaat accagtaagc gacactagca
     1381 acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc cgatttgttg taaagtgacc
     1441 gataaatctt cggtaaaaca gatggggtct tgaagatgcc aacgataaag accatgtcgt
     1501 ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct
     1561 cttgtggcat ctctatttga ttggtaagga taccctaaaa aggctgttga atagtttttc
     1621 gcatgtggac gtccgaactc atcgtactca tgaaaggccc acgccccgcc aaaataatct
     1681 tctgaacctg ttgaggttac ggtcggatac tcactatccc catcaatgta gaatttaaac
     1741 tcgccttcgc cccaccaata acgttctagg caggtaagcg caaggtaagt acccacgtaa
     1801 tagccttgac cctttaatcg atcaattaac ttataatcct cctgtaattt ggtaatgcgt
     1861 tcccgatgcc aatttgcgtg aaaatatagg ctattttctt ccaattcgtc caccaaagcg
     1921 taatttatcg tataaaaaaa tgaggacata tccttcggat gtaaattctc gatcgtaatt
     1981 ttagcggatg tttggaaagg catttcgaaa taggaattaa agccccctgt aggattaacc
     2041 actatcggca acgagttcac ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct
     2101 aaaggacttt caacagcagg gctatccgac ccatcccaat aaattcttaa gattaaatca
     2161 cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg tcatccacat atgacgaatt
     2221 attcccgttc cagtaatatc cgcaatcaca gtttcttcac cagaagtcag cggaattgcg
     2281 ccacgacctt tgcggcttgg tcctagctcg cttggcaaca tcgcagcttt acctttctcg
     2341 cctgttggat tttcgggagt gattgctcga ctctggattt ttttaaattg ggtaatattt
     2401 tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa
     2461 gaattttttt cgtatcactt attttcaatg gcttttctga caaatttgaa atactcaact
     2521 cggattgcgt acattcaatc gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa
     2581 acgttaaatt atcaacagga tcaatttgca atactccttt tttgaatttc aatccgaaat
     2641 attgactgat cattaggctg acaaaaagac ccgatgccca gccgcatttg ccgcagccaa
     2701 agtctcgcac gacttctcct tgttgagcct ctaagcgata cggccaccac caccacgaac
     2761 cgtctaaatc tgccaaacaa attagttctt ctaatcgggt tacgaaagct tgagggttgt
     2821 gcagctcgct cattaaaaca gtgataaagc caggaaaagt ggcgccggat tctaatcccc
     2881 aagtgatgcc tttgatttcc tcgctatagg ttggattgaa agaactagca gaaaaggcca
     2941 tggtgttctg atataaatga tctttattat ctaaaaattg ataaaaaggc atcaacgtag
     3001 tgtctgattc ttctccatca tgcatcaata aattaatttt tccatccaca atcacatcac
     3061 ttaaaaagat taacccttgc tcaagaatcg gtttttgata gtgcttcact gaataggttg
     3121 ttttctcctc atcaccgatt ccctccaaaa attgctcacc aaaaggacca tcgatagtca
     3181 tttgtttaca aattgattcc tttagtttac aggcaatgtt tttgtatcgc ttgctaagac
     3241 tcagttgttc tagggcttca aaaatttctg caagtccaat acaggccttc caaagacaaa
     3301 gattgctgcc ggtatgatac tttccaagtg caaaggcgtc tgataaccaa acagatggaa
     3361 aaagcattgg ttcccctgac cgacgttgag ctaatatgtc ctcaataatt cttgaagcat
     3421 tcagtagaac ctgcggacac cgctcaaaga attttgtatc cccagtatgt tcaaaataaa
     3481 gactagccag taacgcactg gataacgaat tactcagaga atgattgata cctcctgtaa
     3541 attttgaacc aggaaactta acgccatatt gatcaaacca tagaaccgtt ttttgacata
     3601 actccggttc aaacatggtg aatggcaatg atgcgtaata catatcctta ttccaaattc
     3661 gattagtagc aggatagctg ccccaattgc tgccgacgac ctgtccctgt cgattcatac
     3721 cgaaactaga aaagctttga taaagtgcac gacgatagag atggacaact ctatcgtttg
     3781 gcattgacag ctgcccaaat agctgagcaa aatagttcaa tgtttccttc agccattttt
     3841 cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc atttgggtcc acaaaagcaa
     3901 tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca
     3961 acacattttg ctgatccgaa tacttctctt gatacgaagg cacatctttc actgaaactt
     4021 gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg aacaagcata cttaaacgct
     4081 tattagccag tatgggagca aaggatacta caatgacctt aaacgtcgaa taatgatgaa
     4141 tatatctagg caaacagtct tgaatcacat cgacttcaat acgatgatcc gattctgaca
     4201 aatggtagac aatcccgttt acttcaacct ttaaggataa cgaattggtt tgagagagct
     4261 gtttattgac ccaaacacct ggtttttcct cagttagtaa attttctcga taaaaggtca
     4321 acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt cttttgatta cttacatcga
     4381 aggtcaatcc ctttacatca ggaatattcc gatagtagct gtattcgttt gaagtagtca
     4441 catttccgtc gtttaactca gcattaaaaa atgggtgttt gggtgaataa attacttcat
     4501 taatagtcat cttttccctc cttgctaaaa aaactgcagc ctagtagctg cagcttccta
     4561 ctatgcgatt ccaagtaatg acaataaaat tcccaagacg accattccaa aaataatttt
     4621 gatgacgccg acttttttat ctagtaattt gaaacagctc aatgttacca ataacggtac
     4681 aattccttta aaaatactat cgaggatttc ttggacattc attagttctt cgcccttcat
     4741 tgaaaagctc aaaacggtat tgaatttgac catactgatc gtcattgcac ctaccatgat
     4801 cagacccaag atactagcag cttttgtcag aatttcaatt aacccgttcg catacagctt
     4861 ttcaatatat ttagagccta atgaatagcc taaaacgccg ccatagtatc gcatcaagta
     4921 attaggtata ttgaacataa ctaaaaagat aattggtgcc aaaatatttc cggcattggc
     4981 taagttgatc gctagtcccg cggtaacgac acgccaaact ccccagaaca atgaatcgcc
     5041 aattccagcc aatggtccca tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa
     5101 accagctttt tcagaatttt ccttctccat cgatgccgca atccccatga caaaggacgc
     5161 gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc agcgccgcag ctcgatcttc
     5221 ttcacttttg tagaaatgat taatgaaggg catcatagaa taggcgaaac caccgccgcc
     5281 catcgtcacc ggattcaccg aagcatacat agtcatcgag cgccaaaaca tcttccgaac
     5341 caattttttc tcatctttag aaattccatc agctaatttc attgaaagaa atcctcctct
     5401 tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa ctgcatgaac agatatattt
     5461 tttagttgta gatctctttg ggctataaca attgcaataa tcgttccaac tgcggcaatt
     5521 gcaatcaatg gtaactcgag atacgccata acaacggccc caaagaaaaa ccacacagca
     5581 atatttttac tccaaagcat ccgtaacagc atcgccatcc caactgccgg cagcagattt
     5641 ccagcgactg ttaatccgtt catgatgacc tctggaatat tatccaagac gcttttgaca
     5701 acatctgctc caaataaaac ggcaaagaat ggaataagag cataaagcgc atagtggaca
     5761 aaccacataa agaagtgcag attgatgatt cctttttgat caccagattt tgcaaatttg
     5821 aaaaatttag cagccatcgg tccacataca aaagaaaaaa tcatattcat taactgtaat
     5881 cctaatactg ctagtggaaa gacaatggga ataatcgcat ccacgtttcc tcccattttg
     5941 attgcgaacg ctgtagccag cccagcagcc aaagaaggct ccgccgtact agagattccg
     6001 atattgacga cacccataaa gatggcttct agtgacgccc cgacaatcaa ccccgtctgc
     6061 aaatccccca tcaacgctcc tgctaacgga gcaacaacaa tcggacgact cagcatggac
     6121 atcccgaaca tttcatgtcc agcaactcca ataaaaacgg ccaaagcgac tacaaatgca
     6181 tcatacataa tttttctcct tcctttttct aaaaatcact caaaatagag atagctgaac
     6241 gtttttccat cgccggtgta ggctgaaaaa cggtatctgg ataaagttca actaattctt
     6301 ttaagtcttg aatttctgac gtcgttaaca tcacttcctt tgataaggtt ttcttatctt
     6361 ccgatggcgg tgtcattttc ccagcattcc ccacgttcat taattcgatt tccgttggga
     6421 aagctttcgc aacttttacc gcatctttga cattagggac taaaaccatc aagcgcatgt
     6481 ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc aactgtttta ataatgactt
     6541 tgacattcga tggtgcagcc atcttcaatg tcatcttttg aatatcgttg gtagccgcct
     6601 catcgcttgc tactactaac ccggttaaat ttagactctt tgtccaagtc atcgctactt
     6661 gtccatgaat taatcgttca tcaattcgca ctgctttaat catttttctt cctcctaaaa
     6721 aaaatcttcc tcttcattta ctacatgtac ctcatcaaga atggtcatct ttaattcctg
     6781 ttggcattta tttaccagtt cctgaatatc ctcctttgtt aattccttgg ttgccaatac
     6841 tatttcgagc agaattggca gattaaagcc agacaaaata gttacagcaa tctctttctc
     6901 caaaaaggtt ttggtaatct tctgattgac acttccgcca aataaatcgg taaaaacaaa
     6961 aatctgatcc tgctcgttca cctggtttac aaatacattc aactcctgtt caaaatcaca
     7021 ctcatccaca taagcattaa tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat
     7081 actgctaaga aagcctgaag ctaagtgacc gtgagtcgct attagaattt tcttcatatg
     7141 gttaattcct ttctttctgt tcacttttat ataagcaatt cctgtgccaa cttttaaaaa
     7201 cattggttta atcacgttaa ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt
     7261 agacactttt ttagacactt tatttttgaa tatacggtcc taataattca tagagcagcg
     7321 aaagctcata agcattgact ttaaaattat atttcatctc cattgattga aaaatacttc
     7381 tagaaacata ataaaattca tcttcatctg gtttcaaatc ataattcggt tcttcagatt
     7441 tttctgtccg agccaagaat aaacgttcca tcatcaatgc cgtatgcata tagagattta
     7501 gttttacacg gccatttaaa gtgaaatgat agtaattttc atatttagaa atgactgttt
     7561 ccacttcact catcacgatc gttgggttca gaaagttcaa acggtccgca attccttcca
     7621 acgaaaagaa ttttactagc tcctgaatca agcggttaaa ttctgccggc ttcatttcag
     7681 gactcaaaat atgctccaaa ttggctgctc catcaccgtc taagatgtca taaacattaa
     7741 tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt gatgaccaat tttgtcttgt
     7801 caaaataagt ttcatcattt tggtcgatta attcacgtag ttctctgtag tccttcgtga
     7861 aaacttcgat ggattcatcc gataaatgat gacgaaaaac atctttcaat ttgtccgaaa
     7921 tccccaaacc cgacatacag gaaatgataa tgttgctact ttgagaaaag ccttcaaagt
     7981 attgaacatt gattgaatac ttgtctgctg catcctccgc aatctgttta aatggcgcat
     8041 tcatcgtcat cttcaagcca atatcaagtg caatggaagt cgtcaaatta ttcagaagaa
     8101 gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat ttgatttaaa gagcccatat
     8161 ctacgatcag cactagacta tctgtattca cttgccgttt cacgaaattc cttgtcgatt
     8221 cgacaatctc tgacacatta ctctctactg gcatatccaa cgcttcaaaa acataattgc
     8281 cgcataattg attcactact gcttgaatgc tgctagccgt actatctccg tgagcaagca
     8341 acaggccctt cagctcgatg gattcatcta cataatcaga taaagtcacg acaataagca
     8401 gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa atgctccaag aactgattac
     8461 ataagtaagt cgtccgaggt agatttgcag ctaatgtacg atttatcccg gttaagtctg
     8521 tcttatagct tggggaaatg acataggatc tgctaataat ttctgcaaca ggtccactcg
     8581 tcgtcaatcc gtacgttttt cctaaaacgg atacccagac ctttctaaac atctgaatca
     8641 tcaattttct actcattgat aaaaaagaat ccttatcaat gtgatgcacc aaatgctgta
     8701 cttcattctt tatcgaagaa aaattttcat tgaaattacc ttcatttatc agctgagtaa
     8761 ttttttccaa aatcgaatca taatcaaacg gtttgattcg atccgttttt atcttttgat
     8821 ttttctttat caataaacta gcaatcggca agctttctac ttcggattcc agcatattta
     8881 actgaatcaa gatctcattt ttcccaatcg ttaatggttc ttcttgtgtt cgactataca
     8941 catccgcaca actgatttta atgatattct tcagcttacc aatattccca ctaaagggat
     9001 gagataacat taaagacaag gcttctcttg aaacaataat atctttttgt aaaacatccg
     9061 cttcattttg aaaaaataat tgaatcagct ctaatcgttc tcgaatgggg cgatcttcaa
     9121 aagttggcaa cataacacta atttgaatac gccgatcaaa ggtgtctaat aaataatttt
     9181 cacctttttc cgtcgtggcg aaaataaaac gaaccttagc agaatgccaa tttacatttt
     9241 caccgactgg acgatagttt ccagtatcaa taaatagaaa gagcttttct tgattttctt
     9301 tcgataggcg atggacctca tcgagaaaaa gatagccgtt gttggccgtt gccaataaac
     9361 catcggtatc cttttcagcc cccgtaaagg cccccttctt gtatccaaac agtgttgccg
     9421 aaagcagttc gggattgtcc gcataatcgg cacagtttaa gatagcaaat ggtgcatctt
     9481 tttcaataat ttgctcatgg atagcgtatt caaaaatttt tgtggctaaa aaactttttc
     9541 caacacccgt ggctccattt attaatacac tgagtccgtt aggcggatat ttgacagcgg
     9601 ctatacattt ttgaatgacc tctctcagac ttccgtcatg accaactaca gaatgaaagg
     9661 cttcgtttat ttgctccgta ttatctacaa ccgcatattc atctaaaggc ttccacaaaa
     9721 caggctttcc cggtaatttc tcgacctttt tatcttccac taatctcgtc aaataatggc
     9781 tggcattctg acgagagata cctagattct tcgaaatttt ctcggcagtg acagaagaca
     9841 tattccctgt tagtcgtatc agctcatcaa aaactcgatc tttagcgctc tttttcattt
     9901 tttcctccag tttaatgtgt ctaaaagcat aataaactat cggctgcaat atgtctattc
     9961 tatatttgta ttccaatttt tactttgaaa cacaaatagt cgaatatttg cctcagatat
    10021 ctgacaaaat aatttatcta ccaatttagt ttttatctaa tatgattttt atgttttttc
    10081 atagctacaa atattgaact ggat
//
LOCUS       DAZKCZ010000088        10015 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000088 DAZKCZ010000000
VERSION     DAZKCZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.089"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(479..703)
                     /locus_tag="ACG8KN_002058"
     CDS             complement(479..703)
                     /locus_tag="ACG8KN_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189078.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            894..3671
                     /locus_tag="ACG8KN_002059"
     CDS             894..3671
                     /locus_tag="ACG8KN_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9189079.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3752..4171
                     /locus_tag="ACG8KN_002060"
     CDS             3752..4171
                     /locus_tag="ACG8KN_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9189080.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4183..4653
                     /locus_tag="ACG8KN_002061"
     CDS             4183..4653
                     /locus_tag="ACG8KN_002061"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9189081.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4670..5449
                     /locus_tag="ACG8KN_002062"
     CDS             4670..5449
                     /locus_tag="ACG8KN_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9189082.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5436..6257
                     /locus_tag="ACG8KN_002063"
     CDS             5436..6257
                     /locus_tag="ACG8KN_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9189083.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6274..7323
                     /locus_tag="ACG8KN_002064"
     CDS             6274..7323
                     /locus_tag="ACG8KN_002064"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9189084.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7336..8340
                     /locus_tag="ACG8KN_002065"
     CDS             7336..8340
                     /locus_tag="ACG8KN_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9189085.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8973..9992
                     /locus_tag="ACG8KN_002066"
     CDS             8973..9992
                     /locus_tag="ACG8KN_002066"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9189086.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3360 a         1651 c         2093 g         2911 t
ORIGIN      
        1 ttcttgcaat cttttttgct acgtaatttt tgtaaaaaag tgggttgaga gaaatctaat
       61 tgattttcaa agaaggagta tgaaaatgag aaaaatattt attcgtttat tgtgggtact
      121 atcttagagg aataaataat agtggagaaa actatggttt ggcgcagatt ctatcaggtt
      181 tgatggaacg aagacaacct cccgacatca agtcaaaagc tacttgagat tttgatctga
      241 aaataaaaaa agaaaacaag gtaggaacat caatgtttct accttgtttt cgtaatcaat
      301 gggaaaaaac taagagtggt gtacgtaatc aataaaaagg atggtgacaa cagtacctct
      361 ttactctctg aaagcatcat aactcgtttt tcctcattat gccaataaat tgttgttata
      421 ccacatataa ggatttgtaa tagctaaaaa cagacaggaa agttgaaatc tgtgaatctt
      481 attttgtatg gaaggattta tagaacgcaa aaatcaagat aaagatgaat aaaaatcctg
      541 tttcggctat atattgcgta tctacgccaa taaaacgagt ttgttctgtc atagccatat
      601 aaagcaaagg caaaaataca actacagcga gataagatcc gatgatttct cgaaaagttt
      661 tccaggaagt atgtttcctg tttttatatt tatccgattt cattgaatag tcatagcctc
      721 cttattcttt catatgttga aattccagta taaaaactgg aaggaactgt acgtttttta
      781 gaataaaaat tataagggat aagtaggctg ctgtctataa taagatatat ttttcaattc
      841 tttattaaag attttattga caaaatgaat ccgttttcta tgatggagga ggggtgaata
      901 agttgttgaa gaaggaaatt ttatgttact tagagaacca gacagccttt ttcgatttag
      961 aacatatgaa cgaggtcttt acagcacaaa ggttagctga tcattttggg gtaaaaagaa
     1021 acacagtcag tcattatttg aaccagttaa atgatgagca actactagtc aaaatcaatt
     1081 ctcgaccagt gatttatttc cacaaagaag cttttgaaaa acaatttttt aagttacgca
     1141 cgaattttta tttttctatc gatgatctca aagaggaaca gccttttttc gcgcaaccaa
     1201 aagacttatt ctcttttatg atcggatacg acgccagttt gaaagaaagt atcgaacaaa
     1261 tcaaaacagc tctttactat ccagatggcg gattaccact tttgattaca ggcgaaagtg
     1321 gcacaggtaa aagttattta gttaatttgg tctatcagta ttgtctgctt catgatctgc
     1381 tagaagattc tgcaccattc atcactttta attgtgcaca atatgcagat aatcctgaac
     1441 ttttgactag caatttgttt ggacatgtta agggagcgta cactggtgca gaagaaaata
     1501 aaaaaggggc ttttgaagca gcagatggtg ggatcttgtt tttagacgag gtacatcgct
     1561 taacaccaga aggtcaggaa aaactgttca cctatcttga tcaaggtgtg atttatcgta
     1621 tgggagaacc taatttgtca cgccggatca aaacacgtct cttttttgct acgactgaag
     1681 agattactag tcatttctta acaactttca ttcgaagaat acctatccga atcgaactac
     1741 ctgcacttaa tcagcgtagt cgtaatgaac ggctagaact gatttactct ttttttctcg
     1801 aagaacaaag aaaaatgggg agccctttgt cagtcagtgg accagctttg tcacttttaa
     1861 caagtcagaa attatccgga aacataggag agctgaaaaa tatcgtcaaa gtttcagcag
     1921 caaaagcata tgctgaacag cgtgaacagc ctgaaatcca tgtcacgatc catcatttgc
     1981 aaaaagaact acttgcgcag cctattacta aaaatacagc acagcaagga atctatatta
     2041 cgcaagacca gaccttagag caattaatgg agaagcaaca gcccgaacag cagcgaatcg
     2101 ttaaaagttt tgagcaaatc ctaattgatt tcaagaaaaa taactgtctg ctatattctt
     2161 gcgaaggaaa attaaagcaa gaagtgattc aattatttga ttttttgctg tttgagacca
     2221 gtagacaaga aaaacacgag ctgctggttt attggacaca atctatccga gagacctttc
     2281 gacaaatgga aactgcctat caaatcaaat tcgatggaaa cagtgtctat gccttgagtt
     2341 actatctcta tcagcgaagc actatcaaat ggttaccgga agaacatgat attatccaac
     2401 taatcaaaga attagaacaa caagtttctc agtcttatcc atctagttat cactatgtcc
     2461 aacgaattct ggaattaagt aagatcaaat tagatattga agacacaagt atggatcgga
     2521 ttttactgac gatctatcta aaaaaagcga aatggtcaaa agaaaaacga ctgccaaaag
     2581 cgatgattgc tgctcacggt tatgcaactg ctagcagtat ggcaaatgta gtaaatcgtt
     2641 tattacaaga ggatctattt gaatcatttg atatgccact agatgtcaca ccacagcaga
     2701 ttgcttcaga aatccttgat tattgcgaaa acagcgatgt ttcaaatgga ttgatcatat
     2761 tagtagacat gggatctctg aaagaaatcc atcagttttt taaaaaacag ttatccgtac
     2821 cgcttttgat tttgaataat gtgacgacac ctttagcaat cacagtaggg gaatgcctgc
     2881 aaaaaaatgt atcattagaa gagatagcag aagagactgt tcgacagatc cagccagaat
     2941 ggcgactgct ctatccagag gaaaacaaac ccaaagcact cattactact tgcttcactg
     3001 gaatcggtac ggcggtccac ttaagtgaac tgctagaaaa aagcttacct acggcttgtc
     3061 agttgaaaat catcccatat gaatatcagc aattaaaaga taagaaaaac agtgacccat
     3121 tattttccat atacgaagtc gtaggaatga ttggcaccac agatcctgat atcaccaata
     3181 tcgactatct ttcattagaa gacctcatct cgggggaaaa gatgtctgta ctagctgaat
     3241 ggttggacac tactatgaat gcttcagaga aggaaatatt caatcagcgc atcattcgta
     3301 atttttcatt ggaaaaagta ctggattccg tgactattct agatacagag aaggtgatgg
     3361 gagaaatcga tcattttatg agagaattag aggtgcagtt gaatcgaagc atcagcaatc
     3421 ccaaaaaatt agcgttgtat gtccatgtga gctgtctcat tgaacgattg atccgacaaa
     3481 tgccaatcga aacctatcaa ggattagaga aatggaagca gtgtcaaaaa gaagggttat
     3541 ctgctattaa atctgcgttt agtgtcattg aagagcatta tagtgtcatg atccccaatt
     3601 ctgagattgc ctatatctat gatattttga gtgaagatac agagtttctc tcgatagaag
     3661 aagaattttg aacataagca agttgttgcc actaattttg gcatgcaact tgctttttta
     3721 gtatgtgtaa gtgaaaagag gagatgaaaa agtggataga gaaattatct tagcttccca
     3781 tggaaaattt gcttcaggga tattagattc attagagctg atctatggga aaaatcattc
     3841 tattactgta ttcgattgct atactgatga gaattttgat ttagcggaaa cagtaaggca
     3901 gctttttatg aggtacaaag ataaagaaat cattgttctg acggatcttt ttggcggaag
     3961 tgtcaacaac gaattcttac aatatatcaa tcgggatcat gtctatctcg ttgccggatt
     4021 gaatttgcca ctattgattg agctagtatc tcggttggac gctgaagtag agacaactgt
     4081 tttgatccag caggtgctaa ctgattcaaa aaaaatgatc cagttttgta acgagcggct
     4141 caatcagaaa atagaagaag acgaatttta ggaggaagaa aaatgattgt attgacaaga
     4201 gtcgaccatc ggttattaca cggacaagta gctttttcat ggacacaaac aataggcgca
     4261 gactgtattt tgattgctaa tgatgatgta cctacgaatg agatacgaaa gacaacgatc
     4321 aaattagcca agccacaagg agtcaaacta gtgatcaaat caattgatga ctcgattgcc
     4381 gcgttgaaaa gcggggtgac agataaatat aagttattca tcgtagtgga atcgatcgag
     4441 gatgcatata agctggcaga agcgtatcca gaaatcaaaa ccatcaactt aggagggatc
     4501 aaggcaaaac ctggtactcg cagcatcgga aaagcggtaa atattttacc agaagaggag
     4561 gaacgcttga aacgattaat tgacaaacaa atagaagtag aaatccgtca agtaccagca
     4621 gataaaaaag tggatgtaag agaagttttg taagaaagga agaacctcaa tgattgtaca
     4681 agcagtctta ttaggaattg ttgcatttgt cgcacaatca gaatatgcac tagggacatc
     4741 gttgctttct cgtccgattg tgacggggct gtttacagga atcgtgttag gtgatgtaaa
     4801 aactgggatc attatgggcg caactttaga gttagcattt atcggatcgt tctcagtagg
     4861 tgcttcgatt ccacctgatg ttgtgaccgg tgggatttta gggacggctt ttgctattac
     4921 agctggagcc ggtacagaaa ccgcgctttt actaggtttg ccgattgcta cgttgacatt
     4981 gatattgaaa aatatttact tagggctttt gattccaatt atgaaccata aagcagacaa
     5041 ttatgcagaa gaaggaaatt ataaaggagt cgagcatatg cacttgcttg ccggtatcgg
     5101 tctttccttg atgcttggtg ttgtggtaat ggtttcttat atggtgggaa gcaatacgat
     5161 cggaaatcta ttaaatatga ttcctgactt cgttcaaaag gggttgtcag tagctacagg
     5221 gttgattcca gctcttggtt ttgcgatgct tgctcgtttg ctgatcaaca aacaagttgc
     5281 accatatttt ttcttaggtt ttgctatagc ttcttactta gagattcctg tgaccggagt
     5341 agctattttt ggtgcgattt tagcagttgt ggtagtcaat attatgaatg tccgtcaact
     5401 accacttcaa acaactgcag gggaggtaga agaagatgaa gaattctgag atcaaaaaca
     5461 gcaaaataac gaaaaaagag ctaaatcacg tattttggcg ttcttttcaa atggagtttt
     5521 cttggaatta tgaacgtcag atgaatatgg cttatgttta tgcaatgatt ccgattttga
     5581 aaaagttata tcagtcgaaa gaagaaatgg cagcagcttt gaaacggcat ctagagtttt
     5641 ttaacacgac cccacatatc gttacactga ttttaggaat cacggctgca atggaggaag
     5701 aaaataagga tgatcctgaa tttgatgtga ctgcgatcga tagtatcaaa acatctttga
     5761 tgggacctct tgcagggtta ggagattctt tcttttgggg aactttgcga ttgattgcca
     5821 caggtgtggg tacctcttta gccatgcaag gaaatatttt aggccctatc ttgttcattt
     5881 tgattttcaa tattccgcac attctgtttc gctatctagc aacaggttgg ggatatcgct
     5941 taggaacggg ttttttgaaa aagatccagg caaacggtat gatggaaagt ttgacattag
     6001 gcgcgtctat tattggactg atggtcgttg gagcaatgac tgccacgatg atcaccatca
     6061 atattccttt aaaaatcggt tcaggagaaa atgctgtgac tgttcaaagt atttttgatg
     6121 atatcgttcc aaatatctta tgtcttggag catttggcat agtgttttat ctattgaaga
     6181 aagaagtgaa accattaacg atcttgatcg gactagcttt ctttggaatt tttggttcgt
     6241 ggatagggat tttttaaaaa aggagcagag aaaatgttta cgatgcaaga ttatatctat
     6301 gaagaaaagg aagtactaag tacgatcttg aaaaagaatg atttttcgac aagagaacat
     6361 atgaaaaaaa cagtgaattt actgatctta gcaacgggtt catcttataa cgcttgttta
     6421 gcagcgaagc cagcattgga atcttatggt gatcttactg tcgatattca agaacctttt
     6481 tattttttca attatggaaa actttctcct tcaatcgata ctgtgattgc tgtgtcacaa
     6541 agcgggaaaa gtgcttctac tgttgaagca gtaaaaatga tacaaaaaca agggttacct
     6601 gttgttgcta tcacaaatga cgtgcaaagt ccacttgctt tagaagctgc acaaattatt
     6661 gatttaggtg ccggggtaga atcagtaggc ttcgttacaa aaggctattc tgcgactgtt
     6721 ttacagttac tactcttagg gataggaatt ggaataagta aaaataaaat cagcaaaaaa
     6781 atagaacaag actacatgca acagttgcgc aaaataatca accatttacc agcgattatt
     6841 caaaaaacgg aagaattttt tgatggatat caaagtttat ttcgtttagc acagcgtttc
     6901 gtcaccattg gatatggtcc aaactgggga acagctaaag aagcagagac gaaattgact
     6961 gaaacaatcc gcgttccgtc acaaggattt gagttagaag cttatatgca cggcccatac
     7021 ctggaggcag atgcttcgca tttgctattc tttattgaag gggaaagtgt aaataaggaa
     7081 cgttcgcaaa agttgcaaag atatatgagt cggtacgttg gagagactct caccattacc
     7141 acaaaaaaag caagaaacga gaaaacactt ggattagcaa tagaatgtga tgaatatctg
     7201 tctgtattag cgcttgtcgt tccgtttcaa ctattcgctt ataaaaccgc agtagctaaa
     7261 ggtattgact tgaacaaaaa gatttttgaa gactttgata cagtcttgaa aagtaaatta
     7321 taagaggtgg aaatcatgct gaaattcaat gaagaagaac aaatcaaagc aataaaaggc
     7381 gcactagctt tacgtccgca agtagaagaa atcatcgata aactttatgc ggaaaaattt
     7441 gacgctgtct attatctagg aatcggtgga acgtatgctt cctcgatgca agctgtcact
     7501 tatatgaatg ggaaaagcaa cttgcctgtc tttgtacagc atgctgcaga atattacact
     7561 actggaaaca aaagattaac aaaagattct atcgttattt tgtcctctgt taccggtaca
     7621 actcaagaag tagtgaaagc agtagaacaa atcaagaaag taggagcgac actgattgga
     7681 tttatcgata aagcgaacag taagttgtct cagctatgcg attttgtggt cacttatcca
     7741 gctcctggaa cagaacaaat caaatttttt atggcagcag atcgattgat gtatttgaat
     7801 ggagaattcg aagcttattc agaatactat gaacagctcg aacgttattt accaacaggg
     7861 ttagtggaag cagagaaaaa agcagatgca tttggcttat catttgcaga aaaacatcga
     7921 cgtgatagta tgcattattt tattggagca gggaatcaat ggggagctgt ttattcatat
     7981 gccatgtgtt actgggaaga acaaagctgg cttccatcga aatctatcca tgctgccgag
     8041 tttcttcacg ggacgttaga gatcgtagaa gaaacaaccc cagtcacatt atttttaggc
     8101 gaagatgaac agagagtgtt atctgaacgt gtggctaaac ttttgccaaa aatttgcagc
     8161 aattatacat tgatagacac aaaagactat ccagtagaag gtatcagtga aaaatatcgc
     8221 ggacgtgttt tgtctttctt attgatgcat gtagtgacac aacgcatcga tgctcatgtg
     8281 gagcagttga actgccatcc attagagata cgccgctact atcgtcagtt tgattattag
     8341 gatagactgg ataaaaagaa tataaaaaat aaagagagca gcgataactg ctccctttat
     8401 ttttttaaaa tattggcatc aattacgtta taaaacacat tttttgcatt atctacagtt
     8461 caacttataa tctgtttaat gggacatgtg ggtgccaacc attttttgtt atgcaatagt
     8521 actatttagt tgttgcatgg atccgtagta aaagtccaag tacaacgagt tgcgccagtt
     8581 tacatagcgt tttgtatcca gaatgttgac taaaacaaaa gttcctatgt caaccgtcaa
     8641 cagagacaat ctgtccgttg caggacctga cagatttaga aatgttcaat ctgtctctta
     8701 tgtattttaa atcaatactc actaggagaa gtttcctaac ctcctaatag taatgctggt
     8761 agtaacattg tcaaaactgc ttcctcttag gaacaagcaa agcataacga gtgtaaaaat
     8821 aaaatgtaga ctattgtaac atatcattga ttatttcaag taaaaacgat aaaagaagta
     8881 aacaaacact taaaaacaat ggaaattagt cccaacttta agaaatgtat actataatgg
     8941 aaattatcaa ttatttattt ggaggaatat ccatgaaaca aaaaatcgca gttcttggtc
     9001 ctggatcttg gggcactgct ttagcacaaa cattggcaga aaacgggcat gatgtccgta
     9061 tttggggaaa tgtacctgaa cagattgatg aaatcaataa gtatcataca aatcagcatt
     9121 ttttacctga tttgacgatc ccagaatcga ttatcggata taaagaattg gcagaagcag
     9181 tggataacgc agatgctatt ttatttgttg ttccaacaaa agcgattcgt tcagtcgcac
     9241 aggaacttat tggtaaaatg aatactaagc caatcattat acatgcaagt aaaggactag
     9301 aacaagatac gcataaacga atctcagaag ttttggctga agaaattcca gaagaaaaaa
     9361 gacaggcaat tgttgtatta tctggtccta gccatgctga ggaagtggca gtacatgata
     9421 tcacaacaat tacagctgca agtattgatc aaaaagcagc agcctatgta caaaaactgt
     9481 tcatgaatga ttatttcagg atttatacaa ataatgatgt gattggcgta gaaactggtg
     9541 cagcactgaa aaatatcatt gcaattggtg ccggcgctat ccatggatta ggattcggag
     9601 atgatgcaaa agcagcaatc atgactcgag gattggcaga aatcagccgt cttggtgtcg
     9661 cgatgggtgc caatcctctg acatttatcg gtttgagtgg tgtgggggac ttgatcgtta
     9721 cttgtacaag tgttcattct cgcaattggc gtgccggtaa cttgttagga cagggacata
     9781 agctggaaga agttctagaa aacatgggaa tggtcgttga gggtgtggca acgacaaaag
     9841 cagcagtaga gctagctcaa cagctaggtg ttgagatgcc aattactcaa acaatctata
     9901 atgttttgta taatggagaa gatatcaaaa aagcagcaaa agaaatcatg ttacgtgacg
     9961 gaaaaatgga gaatgaattt tctcttcgtt aaatagatga tcaatagtta atcgt
//
LOCUS       DAZKCZ010000089         9912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000089 DAZKCZ010000000
VERSION     DAZKCZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.090"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..274)
                     /locus_tag="ACG8KN_002067"
     CDS             complement(20..274)
                     /locus_tag="ACG8KN_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189087.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(438..761)
                     /locus_tag="ACG8KN_002068"
     CDS             complement(438..761)
                     /locus_tag="ACG8KN_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189088.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            948..1021
                     /locus_tag="ACG8KN_002069"
     tRNA            948..1021
                     /locus_tag="ACG8KN_002069"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:982..984,aa:Ile,seq:cat)
     gene            complement(1406..1903)
                     /locus_tag="ACG8KN_002070"
     CDS             complement(1406..1903)
                     /locus_tag="ACG8KN_002070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFP9189089.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1943..2752)
                     /locus_tag="ACG8KN_002071"
     CDS             complement(1943..2752)
                     /locus_tag="ACG8KN_002071"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9189090.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            3086..3943
                     /locus_tag="ACG8KN_002072"
     CDS             3086..3943
                     /locus_tag="ACG8KN_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9189091.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            3936..4838
                     /locus_tag="ACG8KN_002073"
     CDS             3936..4838
                     /locus_tag="ACG8KN_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9189092.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            4871..5221
                     /locus_tag="ACG8KN_002074"
     CDS             4871..5221
                     /locus_tag="ACG8KN_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9189093.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            5270..6193
                     /locus_tag="ACG8KN_002075"
     CDS             5270..6193
                     /locus_tag="ACG8KN_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9189094.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            6271..7164
                     /locus_tag="ACG8KN_002076"
     CDS             6271..7164
                     /locus_tag="ACG8KN_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9189095.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            7202..7765
                     /gene="amaP"
                     /locus_tag="ACG8KN_002077"
     CDS             7202..7765
                     /gene="amaP"
                     /locus_tag="ACG8KN_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9189096.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            7779..7967
                     /locus_tag="ACG8KN_002078"
     CDS             7779..7967
                     /locus_tag="ACG8KN_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9189097.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7980..8882
                     /locus_tag="ACG8KN_002079"
     CDS             7980..8882
                     /locus_tag="ACG8KN_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9189098.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            8914..9156
                     /locus_tag="ACG8KN_002080"
     CDS             8914..9156
                     /locus_tag="ACG8KN_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9189099.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(9412..9783)
                     /locus_tag="ACG8KN_002081"
     CDS             complement(9412..9783)
                     /locus_tag="ACG8KN_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189100.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3523 a         1699 c         1878 g         2812 t
ORIGIN      
        1 cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa agaatgacga
       61 tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg ggttcaaagt
      121 agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa atagctaaat
      181 aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt gcgaccgcag
      241 gcacccaaaa caaaactctc accaacgtaa tcatgtaacg gactcctcaa cgattcatat
      301 aataaaagat aacatgttat tgcatacatc acaaatgaaa tgctaggaaa ataaaagaag
      361 aatagatcaa tgaaaaatga aaaagcagaa cagttttctg ctgataagca ggaactgttc
      421 cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat tgatatctaa gtaattcaat
      481 acatataaaa agaggacgat cgcattcact aaaaaggaaa gaatgatgat cccatagcga
      541 atggaaacat atttaacaaa tacttttgtt tgaatacggg catatctttt tcctttatag
      601 ctgagcatca tgacaagaag caaaagggta atcacacctt gaaaaatcat tccaataaac
      661 agaattcctg aggtttgata atttaattgg agaaaataag ctgctgccgc aactaaatcg
      721 aataaaatcg caataaatgg caaaataatc actcctaaca ttgagaataa ataaaagaat
      781 aacatttttt ctaaaataag caaataaaaa ttaaagtaac aaaatagtta tgacacaagt
      841 aaaagaaata agaataacaa tattatttta cttatatttt gaaacttgtg agagtttgtc
      901 attcgtgcta tagttgaaaa gtaacaaaaa taaattttat ttatgatgta cctttagctc
      961 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtac
     1021 ataagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaacag
     1081 cttcttatgt ttttacttgg ctgttcttat tactgcagat gcaagctttt gaataccgtt
     1141 taaggaagag gttgtgaaat cagcgttttg acctgagtat tagcttaaca aacggaaaaa
     1201 tagttcctca tattttgccg tttgttaagc taataccgag ggtcagctga ttgaacacca
     1261 tttaagaaag aggtcgtgag cctgccgttt cactctgtgt cataaaaaag ccaatctact
     1321 ttgatagaaa aagcacgtag gactaaaaca gttctgcgtg ctttttttat tgtgtgttaa
     1381 atatcgtaaa tgaagataga tatttttatg attcgacgat agagacgttg aaatctttca
     1441 acagctctaa agcggattct ccgtgttttc tactggagac taacattgga tcaacaaaaa
     1501 agtctgcttc aggaaaagca ctctgcaggc ctatcagatt agagagtaca cacaagtttg
     1561 tttcaatccc gactacttcg atctcttttt gttcttcttt gctttcaaat aaagtttcct
     1621 ggatttcagc taaagtttca ggtggaatcg taaagtaata ctttttaatc actcgttcat
     1681 tttcttgtgg agaaagaaga gggatcaatt gcaaatcttc tcgttcttct tctttgtcct
     1741 tcagttcaac tggtatatcc cgtgtgaaga gaatatattc attattttgg cgtgctttag
     1801 ctaatctttg agcaatacgt tctgccaatt gatctgactc tggaatataa aattcgctgt
     1861 ttggatcaag aatatgattt tgcatatcaa taataactaa cattttcttt cctcctacat
     1921 tcatatcgtc attttattct ttttaataga agttttttaa aatataagca acaccagctt
     1981 cttcattcgt cagtgttact tggtttgcaa tttcttttac ttgtcgctgt gcattcccca
     2041 tcgcaatact caacccgctg taattcaaca tagcaatgtc gttttcttga tcgccaaaag
     2101 ccatgatgtc actcgattcg attccaaggt aatccgccag aaaaacaagt gaatgagctt
     2161 tagtgatacc tgtaggcgtg atttctaaga aatgcggatc agagaatact gattggatac
     2221 catctgcttg cagatctctt tgtagcttct gtagtttttc ttcactctta tctgttaatg
     2281 ctaatttaag atagctaaga gaaggatctt ccaacatatt taaaatttga tcaaaagaat
     2341 ggttctgtaa attgaaatgt tcataataag ggtcatcttt gactagattt gattcatcga
     2401 atgtcagctg atcatcttgg gtttctcggc tagcaatgat ggatattttc gcttcttgag
     2461 catagaagaa ggcttttctc agtgtatcgc tatctatttt tgaacgtctg agaactttcg
     2521 ccatttttcc atccttcatt tcagcaatca gtgaaccatt ggaagaaatc aaatagccac
     2581 cgattcctaa ttcggaaaaa atatctttag cagaaatgct gttccttccg gtagcgacgg
     2641 ttacgattcc gccattttca tcaaatttcc gaagtgcttg gatattttct tctggtatct
     2701 ttccatttcg atcaagcaag gtaccgtcta aatcaatcgc tgctagtttc attttatctt
     2761 cctactttct ttttacatat ctattttcct gttaccttaa gattaaatga gaatttaaaa
     2821 aaatgaaact aacttgcttt gtcattatga attttttcaa aaaacattct taagaaaaag
     2881 gtggctatat atagtagttt ttggtcaata cctttgaata aaaagccatg aaaaacgaat
     2941 agttagcgct taccgatgcg ttttgtgata aaaaaatttt aaaatattca atgaaaacaa
     3001 agcatatatt ttgctttaaa accaaatgag gaatattcta gatgtagaac aggttagaaa
     3061 aagattagaa aaaggagtga ttttgatgga gaagagatac ggcgtagttc ctgtggtaac
     3121 tgcttcagac gaaaattatg ctccttactt gagcgtgatg atcgcaactg cattagaaaa
     3181 ttgcaataaa acaagacgca taaaatttta tgtgattgac gatggtctat cagaatatag
     3241 taaagaagga ttggaagaaa cggtaaataa atattcaagt aatgcaagta tccaattcct
     3301 gacagtggaa aaagatatct acgaagactt cctagtcagt gaccatatca caactaccgc
     3361 ttacttaaga atttctttac caaacttatt agctaaagag gattataaga aagtccttta
     3421 cttagattca gatgtgttag tactagacga tatcgtaaaa ctatatgatg agccgttaaa
     3481 tggaaaaaca atcggcgcta ttatcgatcc aggccaagtg aaagctttgg aaagattagg
     3541 aatcgattcc gatgacttgt acttcaattc aggagtgatg gtgattgaca ttgatcaatg
     3601 gaataaaaaa gaaattacag aaaaaactat ccattacctc agtgaaaatg gcgatcgaat
     3661 catctatcac gaccaagatg ctttgaatgc tgtactttat gaagattggg aacaacttca
     3721 tccaaaatgg aacatgcaga cttcgctgat ttttgaacgt catccagcac caaatgaaaa
     3781 atatgaacgt cagtataaag aaggaaatga aaaaccttcg atcgttcact ttacaggaca
     3841 tgataaacct tggaatacgt tgaaagatca tccgtatacc aatttatact taaaaaaatt
     3901 agcacacagt actctaacga aagtaggcga gatcaatgaa taaaaaagaa atagcagtag
     3961 tcgcaagttg taacacaaaa ttcgtaccac atttagctgc attatttgtc tcagtattag
     4021 ataattgcaa cccgtctaaa tttgttcggt tctatgtgat tgacgatgat atcgattttg
     4081 aaagtaaaca gcttttacgc ttctcagtga aaaacgcacg aatgaactca gatgtggaat
     4141 tcttgaaaat caataaagaa ttctttacaa atgtggtaat cagcgatcgt attccagaaa
     4201 cagcttatta tcgtattgcg attccggaat tgttccgcgg cacagaagtt gaacgtatct
     4261 tatatatgga ttgcgacatg atcgctttac aagatatctc taaattatgg cgtcttgact
     4321 tcggtgattc aatcgtagca gctgttgaag acgctgggtt ccatcaacga ctggaaaaaa
     4381 tgaaaatttc agctaaatca atgcgttatt tcaactcagg tttgatgctg atcaatgtga
     4441 aaaaatggtt agatgaaaat attacgcaaa aagtattaga tttcatcgaa cataatccag
     4501 aaaaattacg attccatgac caagatgcat taaatgctat cttacatgac cgttggttac
     4561 cacttcatcc gcgttggaat gcacaaggct atatcatggc aaaagcaaaa aaacatccaa
     4621 cagcagcagg tgaacgtgaa tacgaagaaa ctcgcaacaa tccttacatt atccattttt
     4681 caggacatgt gaaaccgtgg agcaaggatt ttgaagggcc aacgaagaaa tattacgaaa
     4741 aatatgcagg gatgacggct ttccgttgtg tagctaaatt ccctaaatat ccaaaatatg
     4801 caaaagttca accaaaaaca gatgtagttg ttaaataata gaagacaaca actgaagagg
     4861 gggagtagga atggaaatta caatgtctaa aaatgcagta gagacgttaa ttgaaaaggt
     4921 tggggaaaat acaaaaatcg cactcgcgct tatcaatgat tccgatccat ttctaaggga
     4981 caaaggtgct tttgcaaaag gcagcttttt tcaaatcatt ccttttgttt cggaatttgg
     5041 agaatatgcg actaaaatcg agcatccatt attggacatc tatacatcta aattagagca
     5101 aaattatttc ggtaaacgat tgaacatgga cttcaacaaa cagcttgaca gcttttcatt
     5161 agaaaatgaa atcgcagtac tcgattataa tatcaaatta aagaactgct tcttcagcta
     5221 atagtttcga gaaagtccaa aaaggaggta tacattgaga tactactttg tggatgaaga
     5281 aaagaatgta atagaaacag atgaaaaaaa atataattgg ctcgtacttg atagtgcaaa
     5341 gcccgaagag attgagcaag caatcgaaga attcgagctt cctgatgata ttttcgtagg
     5401 aaccgaatat ccggaagaag tttctcggat ggagcgcctt tatgagacga aactagagca
     5461 tccattttct ttggtagtga ttaatctaga tcagtcagat aatcgtatcg aaaaaaaatt
     5521 aacgccaata tcttttgtac tttccgataa tctcttgatt acttgtatcg atagaaaaac
     5581 cgaatttgtg gatcggctgc tgacaaagca tggaaatcat ttaaactcgt ttgaaaagat
     5641 catcacgtat acattattag atatttacac ccattacgtg aaagaactaa gagagatgaa
     5701 aaagcgaatt gacgctttgg ataaagcagc tcgtaaaaca acggaaaatg aagaactgta
     5761 taaacaggca gatcttgaaa gagacatcgt atatatcgat cataccttaa gagatcaacg
     5821 ggaaacaatg gatcaattat ggaattctga aactttcatc aaacgtttag acgatgaaca
     5881 gctactctat gatgtccatt tgagacagcg acaaacagaa aaaatgatcg agatctatcg
     5941 agatttatta gagagtattg gtgatctatt caacgggatg atggacaata atctaaatca
     6001 tatcatgaaa tatctagatt cagctgcatt aatcatttcc gttccggcgt tgttcgcagg
     6061 gatttgggga atgaatacag gaggattacc gggtaaaagt tctgctctag gctttatact
     6121 cgtagtcttt ggctctttga tagcagcagt agttttaggt taccatttat accggaaaga
     6181 ttatacaaat taaagaatct atttaaaaca ggtatcaaac aaagaaattt gttttggcaa
     6241 tacaatttaa aaactaggag gaatacagca atgacagaac caaaattaat cgacccgcga
     6301 aacttgtacc atacggagaa atttcctaaa caagatcagg atacaccagc tttacagcag
     6361 aatatgattc ctgttcctga ttgtggagaa gaatcctatg aaggcaataa tcaactagaa
     6421 aaccgccgtg tactaatcac tggtggagat tcagggatcg gtcgtgcagc agcgattgca
     6481 tttgctagag aaggggcaga tatcgctctt caattcttcc caggagaaga aaaagatgca
     6541 gaggaagtag caaaatacgt gaaagttgca ggacgtaaga ttgtcttact accttttgat
     6601 cttcgtgacg aacaagcacc taaagaaatc atagataaag ccgtgagtga acttggcggg
     6661 ctagatacga tggtactaaa cgcaggacag caaatttctt gtcagtctat tgctgagtta
     6721 ccacttgagc aagtcaaaga cacatttatg ataaacatca tcgctatgtt tgctttagtg
     6781 aaagaagctg ttccgcatat cccagcagga ggagcaatcg taacaacaac ttctgttcaa
     6841 gcattcaatc caagcgaaca tttattagat tacgcagcaa caaaagcttc aatcgctaac
     6901 tttacgatcg gtttagcaaa acagcttgca cctaaaggaa tccgtgtaaa tggtgtagca
     6961 cctggtccaa tctggacacc attacaacta gacgaaggac aaccttctga ttcgatccct
     7021 gaatttggtc agcaaagttt actagaacgt gcaggtcagc caggagaatt agcaccagtt
     7081 tatgttttct tagcatcgaa taaagcaagt tatgttacag cccaaattta cggagtaact
     7141 ggtggagaag cgatcaactt ataagaagtt gatcgtttca cccctatgag gaggtgcgaa
     7201 aatgaataaa ggtgtaaaaa cgatcggagt gatcggaaca ttacttttgc tatctgtgct
     7261 tttgtttgcg atactattaa gtgcaaccta tccattgcct tttcagctag agaagtttcg
     7321 attcttttcc atcactaatt catacgtgca acaatatgta ttttggcttg ccgttgcatt
     7381 tgctattttg acaattatca cgttacttgt tttcctgttt tatccaaaaa caatcggaaa
     7441 ctttctactg aaacatggtg atggggagtt gacgttggat aaaaaagcaa ttgaaggaat
     7501 ggttcgttct cacttgcatg aagatgaatt catccattca ccaaaagtaa atgtcaaagc
     7561 aacgaaaaac aagatcgccg tcaatgtaaa aggggagttg aaacgaactt cagcactcat
     7621 tggtaaaaca ggaacattga tgaaagaaat cgaaaatgaa gtacaacaca tattgggaac
     7681 aaaagagcca gtgaaagtat cggtgaaata cacaggcttt gaagaaccag ctaaacgaag
     7741 caatgaccgc aaacgagtag aatagggggg gaccaaaaat gaaagaacta ttttcgacat
     7801 acaagctccc tatcatcttt ggggtacttg gattgatatt agccgtttta tggatcaccg
     7861 tgggcttttt caagacctta ttgatcttga tcatggcagc gatcggcgtc gctatcggtt
     7921 tttatctgaa acaaacgaaa atcttagatc actattttaa atcttaggag gaaacatcca
     7981 tgaataacaa taacaaaaat aataacaata aaatgaacaa ccaaaaacca gtgtctgcac
     8041 atccagaccc aacgacacca aaccaatcaa atggtgtgaa cgatgaaaag aacaaaacag
     8101 caccagtatc tgcacatcca gatccagtag tccctaacgt tcaacaagga gtagaagaga
     8161 agaaacatga tgcagaaaag catgcacctc atcatccagg aactccagaa tctaaagttc
     8221 cagaacaaca tgatgcaatc gatccttcta aacctgtgga agagcaaaaa catccttctg
     8281 accatgcgaa agcacaagaa aaacttagtt ctgaccacac aaacggacaa ggaaaacttg
     8341 gttctgatca tacaaataac caaggaaagc atgattctga acaggaagat tcaatgcgcg
     8401 gtgagctgaa ttatgaagat aaagttgtgc aaaaaatcat cggaatcgca attgaacaag
     8461 tagacggttt attatctgca aacggtggtt tcttctctaa tgtggcagga aaacttgtga
     8521 atacagataa tgtaacggct gggatcgaaa cagaagtcgg taaaaaacaa gtagcagttg
     8581 atatggatgt cattgtggaa tatggcaaag atatcgaaaa aatctttgaa gaaatgcaag
     8641 aagtaatcgg caaagaagta aaaaatatga cacatttaga agtgatcgaa gtcaacgcta
     8701 acgtagtgga catcaaaaca aaagaagaat ttgaaaaaga tcaagaaacg gtgcaagaca
     8761 aagtaactga cgcagcgaaa aaaaccggtg aatttgcttc gaatcaaaca gataaagtaa
     8821 aatcagcagc tggaagcggc gcacaaaaag taaaagaaaa tacagaacca cgggttcagt
     8881 aataaggaag taaaacatta ggaggaacga aacatgggat ttatttggtc acttatcgtc
     8941 ggaggaatta tcggagcaat tgcaggagca atcacgaata aaggttcttc gatggggatt
     9001 attgcaaatg tcgtagcagg tttagttgga tcagcacttg gtcaagcatt attaggttct
     9061 tggggaccaa gtattgcagg aatggcaatc gtgccatcat tgattggtgc catcatttta
     9121 gtactaatcg tatcattctt tgctcgaaaa ctgtaaaaag acttttaaaa agaggtcgtg
     9181 aaaaagctgt tcttcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt
     9241 tcatttggtt gggcatatta tcgaagatgc aagcttttga acactgttta tacggaataa
     9301 gaggctgtga caagactttt gtcacagcct cttatttatg cgggcaaatt cccaattaga
     9361 tttttaaaag tttttcctta aatgtgtctt tattgataca gagaagcttc tttaataaaa
     9421 caatgcctcc ctaaattttc ttgagatagg aaaggtgaac atacaaaata atacgaccac
     9481 tttgaaaacc cagatgatcc acaaatggat cgtatgatac tgatgaagta aaaagcaatg
     9541 gatcagaaac tcaaagagat tgaaaaagac aaggaagaaa taatatctct tttttactct
     9601 tgtctgtttt agccaataat agctcgtaaa gtaatctaga caaacaaata gaagtaaaca
     9661 aagtttcgta agagcagcaa tcgtatcatg ttgctctgct agacgattga cgagcaggaa
     9721 aaatgttggt aatggaatat agatcacgag taatgaaaga aaaaagtaaa caataaatgg
     9781 catactattc ctcctaattg tgtgagttcg ccctcatttt aaaataacga tatttgtttg
     9841 tgaagctttt tcgtgtcgta acaaatgtta tctaatgtat ttttaaataa ataatcatta
     9901 ttatcaaaat aa
//
LOCUS       DAZKCZ010000090         9910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000090 DAZKCZ010000000
VERSION     DAZKCZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.091"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            204..683
                     /locus_tag="ACG8KN_002082"
     CDS             204..683
                     /locus_tag="ACG8KN_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFP9189101.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            716..2944
                     /locus_tag="ACG8KN_002083"
     CDS             716..2944
                     /locus_tag="ACG8KN_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9189102.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3073..4716
                     /locus_tag="ACG8KN_002084"
     CDS             3073..4716
                     /locus_tag="ACG8KN_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9189103.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4789..5334)
                     /locus_tag="ACG8KN_002085"
     CDS             complement(4789..5334)
                     /locus_tag="ACG8KN_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFP9189104.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5357..5842)
                     /locus_tag="ACG8KN_002086"
     CDS             complement(5357..5842)
                     /locus_tag="ACG8KN_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9189105.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5832..6644)
                     /locus_tag="ACG8KN_002087"
     CDS             complement(5832..6644)
                     /locus_tag="ACG8KN_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFP9189106.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6783..8315)
                     /locus_tag="ACG8KN_002088"
     CDS             complement(6783..8315)
                     /locus_tag="ACG8KN_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFP9189107.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIISLSPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8424..9455)
                     /locus_tag="ACG8KN_002089"
     CDS             complement(8424..9455)
                     /locus_tag="ACG8KN_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9189108.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3296 a         1834 c         1907 g         2873 t
ORIGIN      
        1 aatattccaa catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag
       61 atggtgtttt tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc
      121 tataatgtcg catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa
      181 taaaaggatg aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa
      241 tcgttgtaga agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag
      301 ggctttcact gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg
      361 tatggcgaca tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta
      421 ttttagattt agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt
      481 atgaaaaatt ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc
      541 gaatgacggt agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt
      601 acgatacact acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc
      661 ttttagatga gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga
      721 gaatttctca ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa
      781 gaaaccgaaa agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg
      841 gaattggcaa gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc
      901 ggggaagaca caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt
      961 gacctgtttt gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga
     1021 ttctaataag gaacaagacc aagaattact agaagctatt taccaaacag atacacttga
     1081 agctgtactg acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa
     1141 attagcagag ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta
     1201 ttacccttct gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc
     1261 gactgtcatc cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg
     1321 gatgttgaat ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac
     1381 tacaaatcga aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca
     1441 acatgcggaa aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa
     1501 aactatgatc caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga
     1561 tgaaactgca aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg
     1621 ggcaaatacc tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta
     1681 taaattatcc actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg
     1741 attgctactg gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga
     1801 gcatacaaaa gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa
     1861 tgctgaagaa attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga
     1921 gggagaagag aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa
     1981 agaacgcagt agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc
     2041 tcaagaaatg tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa
     2101 taaagaagcg cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca
     2161 ccgaccagct ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc
     2221 aatagtagaa tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac
     2281 gtaccaaaaa ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga
     2341 tttgagaaat tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt
     2401 acggatcgat gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc
     2461 agcgattgcc attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga
     2521 tacagcagac cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga
     2581 cggatttcaa tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga
     2641 tggtgctgca cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact
     2701 gttgcttaat attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc
     2761 aaaagaagat atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc
     2821 cgcagctatt ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt
     2881 agatattact gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct
     2941 atgataaaat aaagctcagc gaacgcactt agcagattcg ttgagctttt cgtttctagt
     3001 taccggtaac aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag
     3061 gagtgaaacg atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc
     3121 tatcctgcta gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt
     3181 gaaaaactta attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat
     3241 caatctccat ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga
     3301 ttcggaacga tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa
     3361 gtgattatgg atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg
     3421 aaaaaatcta aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc
     3481 aaagaaccga ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa
     3541 actggacagt attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc
     3601 gagaaattga aagaagaact attcaatatg atccgatggt ggttggataa agggatagat
     3661 ggatttcgtt tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca
     3721 gagaaccctt tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg
     3781 aaacatgttt ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca
     3841 gaagaaggac cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat
     3901 catatccata tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat
     3961 gcattaagcg cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa
     4021 aaccatgatg ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct
     4081 atgtctgcaa aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat
     4141 caaggacagg aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca
     4201 gtagattcga aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca
     4261 ttggatatca ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg
     4321 gaacaatatg ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa
     4381 acgatcaatg tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac
     4441 atgatccgca ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt
     4501 catgaacatc cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt
     4561 atggtaaact taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc
     4621 tggacattac tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca
     4681 ccttatgaag ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag
     4741 ttggactttg cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc
     4801 tctaggacca ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag
     4861 ggttttcaaa cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt
     4921 gtcaccgata tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa
     4981 ctggtcaaaa gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac
     5041 aatttcgaaa ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg
     5101 ttcacaacct tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg
     5161 gatgatccat tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc
     5221 gcttgtgctg catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat
     5281 gtcattaact atttcagtca attggtcttt taattctttt tctgacaact tcattttcta
     5341 aaaccttctt tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta
     5401 taggaaaggc aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca
     5461 ctccagccgg ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta
     5521 agcttcctaa taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag
     5581 caacatatcc ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg
     5641 aaatcaaatc gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat
     5701 aaagaaaagc agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg
     5761 gtacagggat caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga
     5821 tcgttaattg cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca
     5881 cttccggtat gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta
     5941 ctttctgcta ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa
     6001 gaaaaggcac aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc
     6061 tcataatgat caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg
     6121 cctttgatca caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt
     6181 tctgttaacg aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc
     6241 gtaatcatcg taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg
     6301 atcacagctt cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt
     6361 tttgttttta tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt
     6421 ttgataccat ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc
     6481 aaagatatag attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag
     6541 gcgcttagtc cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca
     6601 ccagcaaatg gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg
     6661 aataagagta taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc
     6721 atccaattta aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat
     6781 agttaggact tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta
     6841 gctgttcgcg ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca
     6901 tggacttgct cccaccatcg attccaatcc atttctttat caggagataa agaaataatc
     6961 ggttgtggag aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa
     7021 tctaataaaa agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc
     7081 cgatctttca aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac
     7141 caatcgccat aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt
     7201 cctttagaca gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa
     7261 tctgtaatcc gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt
     7321 gttttcaaac tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca
     7381 tatacttgaa gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc
     7441 tggttgttta tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga
     7501 tgctccttag cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt
     7561 ggccggtgag cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc
     7621 ataggaaatc ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga
     7681 gagatgtgaa aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat
     7741 ttcgtccgcc atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta
     7801 ccagaagtat gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac
     7861 aaagcaggtt gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa
     7921 atttctctca ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga
     7981 tcgataaata cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca
     8041 ccaaggtaag acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata
     8101 ataactttgt ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca
     8161 ctttcgcagc attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc
     8221 tcatcaaaga taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat
     8281 aagttcaaag gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag
     8341 taatatacaa attcaagata atcgtaacat aatcctttat ataataacat agtttattgt
     8401 gaaaaaaaga tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg
     8461 tgataagaaa aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat
     8521 cgattaccac ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa
     8581 cgatatgttt ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa
     8641 atttggcgaa tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa
     8701 ttttcgtccg tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct
     8761 tttggtattg gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg
     8821 gtcaaagata catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac
     8881 agaagaatgg cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct
     8941 ctgcttgttt gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct
     9001 gcatcgatat atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa
     9061 atctcttctt caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat
     9121 tgcttccttg aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct
     9181 gtagtttgga acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt
     9241 ttttctaaat ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa
     9301 tcagtaaaca gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa
     9361 cgattttctt ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac
     9421 ttttcttgca gttgttttct agttttgctg tccattacaa attccctcca atagtgttca
     9481 ttttctatat tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga
     9541 agcaaatttt taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt
     9601 attttattag tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg
     9661 tggttacttc atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct
     9721 tttatttccg aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa
     9781 taatggtttg tctaacgata ttgttgggat aattcttaaa gaagtattag aaatagttgg
     9841 tacttcatta ctttacatga agatttattc tatggtagga gcaattatat tgagaattaa
     9901 tcctttttta
//
LOCUS       DAZKCZ010000091         9853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000091 DAZKCZ010000000
VERSION     DAZKCZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.092"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..2194)
                     /locus_tag="ACG8KN_002090"
     CDS             complement(74..2194)
                     /locus_tag="ACG8KN_002090"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9189109.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2424..3602
                     /locus_tag="ACG8KN_002091"
     CDS             2424..3602
                     /locus_tag="ACG8KN_002091"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFP9189110.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3618..4376
                     /gene="aroD"
                     /locus_tag="ACG8KN_002092"
     CDS             3618..4376
                     /gene="aroD"
                     /locus_tag="ACG8KN_002092"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9189111.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4399..4884
                     /gene="ybaK"
                     /locus_tag="ACG8KN_002093"
     CDS             4399..4884
                     /gene="ybaK"
                     /locus_tag="ACG8KN_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFP9189112.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4955..6208)
                     /locus_tag="ACG8KN_002094"
     CDS             complement(4955..6208)
                     /locus_tag="ACG8KN_002094"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9189113.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6325..7674
                     /gene="gdhA"
                     /locus_tag="ACG8KN_002095"
     CDS             6325..7674
                     /gene="gdhA"
                     /locus_tag="ACG8KN_002095"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9189114.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7786..9132
                     /locus_tag="ACG8KN_002096"
     CDS             7786..9132
                     /locus_tag="ACG8KN_002096"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9189115.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9440..9826)
                     /locus_tag="ACG8KN_002097"
     CDS             complement(9440..9826)
                     /locus_tag="ACG8KN_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9189116.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3144 a         1788 c         2018 g         2903 t
ORIGIN      
        1 ttttgaacac tgtttattcg aaaagaggag cagcgacaat aattttgtcg ctgctccttt
       61 taaatttatt ttatcattcg gaagaacttg tggtcgttcc gtgaagttct tggtacgttt
      121 gcgttttata aagatcaaca gttgattgtc cgttgtgttc actaaagaca ctagttgact
      181 tgtcaccaag agatttttca gcttctttta atttctctaa gccattcttg tagtcataat
      241 cactggtatc aattggatct aatccactat tcgtatagaa ccgaagaaga tcaccattat
      301 tgatttggtc agaaacagct aactgtttat caactttttc tttcagtgaa tcaatttcat
      361 tctgtacttc ctcagtaggt tcagtaatag cttcaccagt ttcgttacta tagatcgtac
      421 caccgtagta agtatatttt ggtgtaacaa aatcaccatc acggaaagaa acaagctgtt
      481 catgatcttt cgagaataga tcttgtccta actgaatata attttttgta tctacaccta
      541 gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc gtaagtgtga ttgattccgc
      601 cctgtgtttg tccagggata tggatcatat aagggacacg ctgcatttgt gcattgtcgt
      661 agtttgtcca agtagaactt gtttttccaa ctaattcagc tagattttta tttcttgaat
      721 tcgagacacc gtaatggtca ccgtaaagga cgatgactga attatcatac aagccgcttt
      781 ctttcaaata attgaagaat tcttgtacgg cggtatctaa ataattggct gtcgcaaagt
      841 aaccgttgat cgtttcatca tttgtattgg ctaacgggaa tcctgcttcg tcattggtga
      901 attttgaata aggataatgg ttagatacag caatgaattt tgaataaaat ggttgttgca
      961 gacgttccaa gtattgtacg gattgttgga aaaatggttt atcatgcaac ccgtattgga
     1021 acgaattgtc atcattaaca tcataataag aagaatcaaa gaaataatca tatcctaaat
     1081 tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt cccatggaat accgcactaa
     1141 tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg gaaagtattt tttccgccta
     1201 attgtgtgaa caaggagcct tgattcaagc caaacaatga attttccatc agcgtttccg
     1261 catcgcttgt ttttcctgct tttacttgat ggaaaaagtt gtcaaaactg aacgtgctgt
     1321 tgctgtggta aagactattg atgaaaggtg taacttcatg ctggagacca ttctcatctg
     1381 tcaatttata atcgatcagg aattgctgga agctttctaa atggatataa atcacatttt
     1441 ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc ataatgttct tttacataat
     1501 tttctacttc tttcatatca ttgggacttg cttctgcccg cacttggttt gtctggtaag
     1561 tttgaacacc gtcataaacc atgaaagcat tcaagccaag atatttgacc aaataatcac
     1621 gagagaatgt tcgagtcagc aattctggac gatcagcttc cgccataaaa agatttcctg
     1681 agaaaatcat aactgctaat gtagagatcg caaaagccat tcttgctcgg atcgggcgag
     1741 ggtccaactt gatttttttt gcaatcagca gaacagtcaa gataataaaa tcgatccaat
     1801 agatcacatc atatgggcga aacagtcgaa tggcactttc tcccagacca ctcgctactt
     1861 tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt gaattctcga taataagtga
     1921 cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac catcatcgtg atataagcaa
     1981 ctctagggcg tctgatataa agagcaattc caagtaataa caatgtagtt gcaatagggt
     2041 tgataaacaa gataaagtat tgaatgccac tttcgattcc gagatggaaa tcaactaaat
     2101 aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa agaaaaaaat ccgagcctct
     2161 tattcaaaaa ggcaggtgtt tttatacatt tcaagataat cttcctttct gcacttctta
     2221 atcacagaag tgtcctgaaa ccatgttaca cagctcactt attctcattt attttactag
     2281 tatgacaata tagtgtcaat tgaaaccaga agtttatgct gtttcattaa ggttttcttt
     2341 aagcttcatg aaaaaatgag cagtaagaca agtcctatgt tttttgttat acttgggaag
     2401 agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa aaaaagagca agcaatcgat
     2461 tgaaaaaagg atatccactg atccaaaaag aagaccttgc tcatccccaa aaagaaacca
     2521 cgcaatgggt ggaattagtt gattcgcaaa accaatttat cgcaaaagga tatctaggaa
     2581 agcagaataa gggaatcggt tgggtattga gccagcagaa catcccattt gatcaatctt
     2641 tttttgaaga attttttgtg aagtcaaaag aaaaaagaac tttctttttt tcagatgatt
     2701 ctactaccgc ttttcgtata ttcaatggag aaggagacgg gatcggcggt ctgattgttg
     2761 attattatgc aggttatgct gttttttctt ggtataacga aacgttatat gcacatcgtg
     2821 aattattgat tcaagctttt cgtgaaatct acccggaaat cataggaggc tatgagaaaa
     2881 ttcgatttga atcagtaaat ctgcctgaga gccagttcat ttacggaaaa gaggcaccgg
     2941 aacccttgat cgtaaaagaa aacggagtaa aatttgccgt ttatttaaat gaaggattaa
     3001 tgactgggat ctttttagac caaaaagaag tacggggatc attagcagaa ggtttcgctt
     3061 ctgggaaaac agttttgaac atgtttagtt ataccggcgc cttttctgtt gctgccgcaa
     3121 tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa acgcagttta aaaaaaacac
     3181 aggaacagtt tgaagtgaat ggtttggatg tcgatcagca aaaaatcatt gttatggacg
     3241 tattcgagta tttcaaatac gccaaaagaa aacaattttt atatgacatg attgtattgg
     3301 atccaccaag ttttgccaga aataagaaaa aagtctttcg cgtagcaaaa gattatgggg
     3361 aactggtcaa ggatagtctg gatattttag cagaagacgg gatacttatt gcgtctacaa
     3421 atgctgcgaa tgtctcgata gaacaattcc aaacaatgat tgaagaggaa ttccatgcca
     3481 aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc aaaagatttc cagacgacgg
     3541 ataccttttt agaaggcaat tatctgaaag tgtttgttta tcaaattaaa aataaccatt
     3601 agaaggagcg atgcagaatg gttcgggtaa agggattgaa tataggaagc ggtagaccaa
     3661 agatttgtgc gcctcttatc ggggccgtcc gagaggaagt attacgtgaa gcagttcttg
     3721 cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga tcattatcgt gaagtttttt
     3781 cttttgatga agtagcagaa acattggttc ttatccatga gatgcttgat ggactgccgt
     3841 tattatttac gtttcgtacc ttagctgaag gcggagaaca agacatatcg atccgtcagt
     3901 atcgcgacct ttatgaacat gtgatacaca caggattagt agatatggtc gatatcgagt
     3961 tgttcaaagt agaaagtcta ggaaaaagac tgatagagga aatcaagtct ttaaacattc
     4021 ctttaattat cagtagtcat gattttaaag aaacaccagc agaccctgtc ttgctttacc
     4081 gattgaatgt tatggaacat tttggagccg atatcggaaa actggcagta actcccaata
     4141 atgaacgaga tgttttgcgg ttgatggagt tgactagacg ggcaaatgct tttgtctcca
     4201 tgcctattat tacgatgtcc atgggaaatc ttggtaaaat ttctcgtctg gttggcggaa
     4261 cgacgggttc cgttatgaca tttggttcct taagcagcga ttctacttct gctccaggac
     4321 aattaccggt caaagcattg aagcaaatta ttcaattatt aaatgaaccg ttgtagaaca
     4381 agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa aaacgaatgc aatccgtatg
     4441 gtcgaacaaa aaaagattcc ctatacagag cacgaatatg agtgggacga aagccaccta
     4501 agtgcgtcat cggtcgctga acagcttcca gaaagccaat ctcgtatatt taaaacactg
     4561 gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga taccgggaga agccgaattg
     4621 aatctaaaaa aattagcaaa agtttcaggg aataaaaaag tagaaatgct ccatttaaaa
     4681 gatttggaag caacgaccgg atacatacgt ggtggctgct cgcctatagg tatgaaaaaa
     4741 ctatttccta cttatttaga tcaaattgca gaaagctatg aacaaatcat tgtctcggct
     4801 ggcagaagag ggctacagat ggagttagca cctcaagata tctgtgcatt aacgagcgga
     4861 caatttgcag atataaaaca ataaatcata cgtataaaag aaagaaggag gccgggatgt
     4921 tgtttgtcca gtctccttct ttctttttta tacattatgc tctttgcccg gcattcattc
     4981 ctgctacatg ccctgtagcg aaagccgctg tgatattaaa gccgccagta taaccattga
     5041 catccagtaa ttctcccgca aaatacagac catcaatgat tttactctcc aatgttttag
     5101 gataaatttc ctttagagaa acccctccgc cagttacaaa tgatttctca ataggaaaag
     5161 ttttgttgat tgatagctta aactctttgc atagttgaac aaaatcttga atttgttttt
     5221 gagaggcttg ttgtcctgtc agatgatcca tttcaagccg ctcaagaaag aaaactaaca
     5281 atcgctcagg caatatacca tgccaagcat tgaccaagtt ctttttggtt gctttacttt
     5341 tttctgtcaa tatatgaata agctcttgtt tggtttgtgt agggaaacaa tccagagaaa
     5401 gtgtcactgg ttctcccgtt ttctctaatt ctttattgac aaaacttgaa caacgcagtg
     5461 cagccggacc agaaataccg aaatgggtga aaagtaaatc catcgtatgt tcagtcaata
     5521 cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg taaagaaagc ccttgaagtg
     5581 ttttctcctg aatatacggt tcatccgata tcaaggggga ctctgtcgca taaagaggcg
     5641 tcaccgtgtg acccactcgc ttcacgatct tgtacccatc tccagtagaa ccagtagatg
     5701 gataagtccg tccaccagtt gttaagataa tggcaggagc atggaactct tcgaattccg
     5761 tgcgtatacc gtaaatttta tgttctttgt ggatcaattt ctctactcga gtagagaata
     5821 gaagcgtcac atctagttct ttcagacgat tgagcaatgc ttcgataatc gtttttgatt
     5881 tatttgttac aggaaacatc cgtccatgat cctcttcttt taaatgtacg ccttgtgatt
     5941 caaagaagtt catgatatcg aaattattcc attgcgcaaa cgtactatat aaaaactttc
     6001 catttccagg aatatgtgca atcaaatcat caacaggacg attgttcgta acattgcacc
     6061 gaccaccacc tgtcatcagt agtttttttc ctgctttttt gtttttttcg atcaagagta
     6121 cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat cccgcttgtt ccagcgccga
     6181 ctacgatcac atcatacagt ttttccattc cctcacctca aggaaagtgt agcatatctg
     6241 aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga aaatgagcta aaatgaggga
     6301 agataagcaa aggagcgtgg aacgatgaca aacgcaacag aatatgttca agcaattcaa
     6361 gaaaagctac atcaaaagga tcaagggcaa gaagaatttt tacaagcaat cgatgaattc
     6421 atgcccacgg ttatgtcttt tttggacact catcccgaat atatcgaaaa gaatatctta
     6481 gaacttttaa cggaaccaga aagagttatt caatttcgag tgccttggca agatgatgaa
     6541 ggaaactggc gagtaaatag agggtaccgt atccaataca actcagctat cggcccttat
     6601 aaaggtggat tgcgcttcca tcctagtgtc aatctaagta tcttgaaatt tttggctttt
     6661 gaacagattt tcaagaatag tttaaccggt ctgccaatcg gtggaggaaa aggcggaagt
     6721 gacttcgatc ctaaaggaaa atctgataat gagatcatgc gtttttgtca aagtttcatg
     6781 ctggaattgt caaaacatat cggcccatcg attgatgttc ctgcgggaga tatcggtgta
     6841 ggagctagag aaatcggcta tttgttcggc gaatataaac gattgaaaaa atatgatact
     6901 ggtgtgttga caggtaaacc tttggatttt tggggaagta agatacgtac ggaagctact
     6961 ggttatggat tagtgtatta tgtcaaacat ctattgaacg aagaaaaaga ttcatttaat
     7021 caaaaaacag tttttgtttc cggaagcggg aatgtagcca tttatgcaat cgaaaaagtg
     7081 caagagctag gcgggaaagt tgttacatgt tcagattcta gcgggtacat ctatgatcca
     7141 gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa tcaatagaca acgtttgacg
     7201 gaatacgcaa accaacgtcc ttctgctatt tatcatgctg gagaatctgt ttggacattg
     7261 aaagaaaaag cggatatcgc tttgccatgt gctactcaaa atgaaataga cgaaagcttg
     7321 gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag aaggcgctaa tatgccatgt
     7381 acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt ggtattgtcc aggaaaagct
     7441 gctaatgctg gaggagtagc agtatctgct ttagaaatga gtcagaacgt ccagcgattg
     7501 acttgggaaa gagagcaagt ggatgagcag ctggatcaaa tcatggaaac gatttaccat
     7561 acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa atctgcttct tggagcaaat
     7621 gttgctggat ttgaaaaagt tgccaaagca atggcagcag aaggtttagt ttgaaaaatg
     7681 aaactttctt ggaaatattg tcagcatttt gtttatttgc ttgaaaaata ataaaaaaaa
     7741 acgtaaagta ttacctgtag agtaaaccta taaggaggag aacaaatggc acacattcat
     7801 tttgactatt caaaagtagc accgtttgtc aacgaacaag agttagggta catgcaaagc
     7861 caagtaacag cagcccacaa cgaattacgg gaaggtactg gcgcaggaag cgacttccgc
     7921 ggctggatcg atttaccaac gaactatgac aaagaagaat ttgcacgtat caaagaagca
     7981 gctaaaaaaa tccaatctga ttctgaagta ttagttgtta tcggtatcgg tggttcttat
     8041 ttaggcgctc gtgcagcgat cgacttcttg aatcacacat tcttcaattt attagatagc
     8101 aacacaagaa aagcaccaca aatcttcttt gccggaaatt caatcagctc aacatatatt
     8161 gctgacttga tcgaagtaat cggtgatcgc gacttttcag taaacgttat ttcaaaatct
     8221 ggaacaacta ctgaaccagc gattgcgttc cgtgtattca aagagcttct tgttaagaaa
     8281 tatggacaag aagaagcaaa caaacgtatc tacgctacaa cagataaagc aaaaggtgca
     8341 gttaaagtag aagcagatgc agaaggttgg gaaactttcg tgattcctga tgacatcggc
     8401 ggacgtttct ctgtattaac tgctgttggt ttgttaccaa tcgctgttag tggtgcagat
     8461 atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag catactcaag cgacaaacta
     8521 gaagaaaacg aagcttacca atacgcagca atgcgcaaca ttctttaccg taaaggcaaa
     8581 gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat acttctcaga atggtggaaa
     8641 caattgtatg gtgaatctga aggaaaagac caaaaaggca tctatccttc aagcgcaaac
     8701 ttctcaactg atttgcactc tttaggacaa ttcatccaag aaggacgtcg caatattttt
     8761 gaaacagttg tgaaggttga aaaacctcgt aaatcaatca caattcctga acaagcagaa
     8821 gatttagacg gtctaggcta cttgcaagga aaagaagtgg acttcgtaaa tacaaaagca
     8881 tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc ctaacttgct agtcaaaatt
     8941 ccagaaatgg atgcttatac attgggttat acaatgtact tctttgaaat tgctgtaggt
     9001 atttcaggtt acttgaatgg aatcaaccca ttcgaccaac caggtgtaga agcttacaag
     9061 aaaaacatgt tcgcacttct aggcaaacca ggctttgaag atcttgctaa agaattgaac
     9121 gaacgcctgt aagataaggg tttttcgcta gatttaaata atcagtgata gaaacatgtt
     9181 cgctttaaaa tgaaaactgg tacccattta ggtactcaaa catactgcat cgtttattca
     9241 gaataagggg ctgtgacaag acgtttgtca cagtcccttg tttgtataga aatcgattga
     9301 ttgccttgct ctttagtttg ttttaggact ttaagaacag tgaggctgac ttcgaaggtt
     9361 aattctgttt ttttgttttc tagaatcagt aagcaaaaaa gcactcactt cgagtgcttt
     9421 tttgcgatat attcttagct taagataggc ctaatttttc ttttacagat gagggtacct
     9481 tatcgctggt aattactttt ttctctatga tccgcttttc gcttgctttc aactgcaaga
     9541 tcgttccttc ggggtaagca gaaccattgg cagagtcacc agaagtaatg acatcgaaat
     9601 ctaactgtct tttttcaccg ttttcagcat aagcatctac gttgtattta aaacctcttc
     9661 caattacttc cccattatct gctttgatat cagtttctgc aggcattgga gctttgacta
     9721 ccgcataata atctttacct acataagtag actgatagta gtcccaacct ttccatccta
     9781 taaaagcaat caatatgata acgatcaacc ctaaaatttt tttcatgaaa gatctccttt
     9841 tttattttta tta
//
LOCUS       DAZKCZ010000092         9683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000092 DAZKCZ010000000
VERSION     DAZKCZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.093"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..636
                     /locus_tag="ACG8KN_002098"
     CDS             13..636
                     /locus_tag="ACG8KN_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189117.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1086..2135
                     /gene="trpX"
                     /locus_tag="ACG8KN_002099"
     CDS             1086..2135
                     /gene="trpX"
                     /locus_tag="ACG8KN_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9189118.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2132..3013
                     /locus_tag="ACG8KN_002100"
     CDS             2132..3013
                     /locus_tag="ACG8KN_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9189119.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3016..3786
                     /locus_tag="ACG8KN_002101"
     CDS             3016..3786
                     /locus_tag="ACG8KN_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9189120.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4065..4919
                     /gene="aroE"
                     /locus_tag="ACG8KN_002102"
     CDS             4065..4919
                     /gene="aroE"
                     /locus_tag="ACG8KN_002102"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9189121.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5097..6260
                     /locus_tag="ACG8KN_002103"
     CDS             5097..6260
                     /locus_tag="ACG8KN_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9189122.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6377..6787
                     /locus_tag="ACG8KN_002104"
     CDS             6377..6787
                     /locus_tag="ACG8KN_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9189123.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6961..9189
                     /gene="topB"
                     /locus_tag="ACG8KN_002105"
     CDS             6961..9189
                     /gene="topB"
                     /locus_tag="ACG8KN_002105"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9189124.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9360..9644
                     /locus_tag="ACG8KN_002106"
     CDS             9360..9644
                     /locus_tag="ACG8KN_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189125.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3356 a         1645 c         2083 g         2599 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      961 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     1021 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
     1081 aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt tatttagcag
     1141 gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg aaccagacaa
     1201 aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta gatgagattt
     1261 atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa aatcttgaga
     1321 tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt caacagctag
     1381 tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa tcattagcaa
     1441 acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg ggggctggat
     1501 tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat cagccgcctg
     1561 ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa gtcggaatct
     1621 tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa gaagctgcaa
     1681 aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag attgcacaaa
     1741 cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat aataccattg
     1801 ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc gtcattccat
     1861 ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat caatatcaat
     1921 tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa aaaccggaaa
     1981 cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct caagctgatc
     2041 gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt acagatgact
     2101 cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt cacaaggcat
     2161 gctttgggca atattaggat tagggatttt catgacttat cggatcttag actttcctga
     2221 tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag ctattactca
     2281 tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga tagcaggact
     2341 cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag ggattttagt
     2401 gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc tttctttatt
     2461 gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt actttgacac
     2521 agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat tcttcttcaa
     2581 tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg ctcggtcgtt
     2641 agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg gtgtcattgc
     2701 attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata aagggattgg
     2761 cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc aagaattgac
     2821 gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc tattgatttt
     2881 agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg ctatcatctt
     2941 agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa cgggttttga
     3001 gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa agtcgtaaac
     3061 aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat cagagaaggg
     3121 gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt caatacgatc
     3181 gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct gatcacaaaa
     3241 gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc caagatggga
     3301 acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag aggtcaaaaa
     3361 cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag actatgtcaa
     3421 gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt atctggtggt
     3481 caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt actattatta
     3541 gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg attaacaaat
     3601 cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat ggaagacgcc
     3661 ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca agatatttct
     3721 gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga agaagtagtg
     3781 gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca ttgacatctt
     3841 cctttgattc atcctatact taaaacaata aacaattaaa catatttggg tgttttgtag
     3901 gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg acaagtaaac
     3961 gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc gtatcaaacg
     4021 ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt tctggacaaa
     4081 ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca ttgatgcaca
     4141 atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa gtaaatccaa
     4201 tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt gtaaatcttt
     4261 cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag gaagcgcaat
     4321 tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc tataataccg
     4381 atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa aagaaaaaaa
     4441 tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct gcgttagacg
     4501 gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa gtaaaagaaa
     4561 cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac tttgccgata
     4621 cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca acagatatcg
     4681 ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg catgagaatc
     4741 tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa gaagcaaaaa
     4801 aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga gcaatcgcgt
     4861 ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg tttgattaaa
     4921 aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa aagtaatcct
     4981 ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa tgataggctt
     5041 ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga accacaatga
     5101 gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag cgggatacgg
     5161 ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag gaagatgcta
     5221 cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac gagcaacaga
     5281 tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt ttcaatcggc
     5341 aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa atcattctat
     5401 cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt aaatatgtag
     5461 ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg caaaaagcga
     5521 cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa gatgccgggc
     5581 gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg aaactgattt
     5641 cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag atcaattcag
     5701 cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat tttggaatcg
     5761 attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc gatgccttat
     5821 tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt gtagatatta
     5881 aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt ttccgaatgg
     5941 atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac gctattttca
     6001 gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt aaagctttag
     6061 gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt tcgattgcac
     6121 gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg aattatgggc
     6181 aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa tctgctatta
     6241 cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca aggcttttgt
     6301 catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga tttaaccaag
     6361 agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc gtgaagccat
     6421 cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat acggtgaaat
     6481 gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca cgaatgcacg
     6541 gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa ttggtcctga
     6601 attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag aagaaacgaa
     6661 gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg atgctgttcc
     6721 gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc agattaacca
     6781 agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg gatcatcgtt
     6841 tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt tattgccaga
     6901 tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg aggtcagaag
     6961 atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc cgaaaccttc
     7021 caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt tgctggtgaa
     7081 gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg agcttatgaa
     7141 gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac cttccgttac
     7201 gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt gcagtcagcc
     7261 gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc gtggtcgatt
     7321 attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg gatcaattct
     7381 cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa gaactatttt
     7441 ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg aatgaatgga
     7501 agtccattat atagcttgct acttcaacga aaagggattg atggcagttt ttctcttggt
     7561 cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat caaacacttc
     7621 aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg gcagttttct
     7681 ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa ggaaatagaa
     7741 aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac gaaaaaggaa
     7801 aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa gatgaatcag
     7861 ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta cgaaagcaag
     7921 tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca tcagtatctg
     7981 gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca gacaactgag
     8041 atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca tgcaattatt
     8101 ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt acagcaagca
     8161 atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta tatctacgaa
     8221 gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg aaagatccca
     8281 ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga agctcagact
     8341 cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat ggagaaagaa
     8401 acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa aactgcggga
     8461 aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg aatagggaca
     8521 gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat cgaagtcaat
     8581 aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt agccaatgaa
     8641 ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag aaaaattggt
     8701 aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat cctacattta
     8761 ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat tagcgggaca
     8821 cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc aaaatgtaaa
     8881 aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga atgcgacttc
     8941 aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag agatttgctt
     9001 tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg gaaattcgat
     9061 gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg gatcgaaaac
     9121 agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa tgaaaacgaa
     9181 gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg agaatataaa
     9241 taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta ggctgatttt
     9301 atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta aattatctta
     9361 tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct gattacggga
     9421 cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg attttatcga
     9481 ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca agcatgctaa
     9541 agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc attatttatt
     9601 ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac gagagtttgt
     9661 gacaaaagtc atgtcacaaa ctt
//
LOCUS       DAZKCZ010000093         8856 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000093 DAZKCZ010000000
VERSION     DAZKCZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.094"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..662
                     /locus_tag="ACG8KN_002107"
     CDS             54..662
                     /locus_tag="ACG8KN_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9189126.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            830..2467
                     /locus_tag="ACG8KN_002108"
     CDS             830..2467
                     /locus_tag="ACG8KN_002108"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9189127.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELYLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2539..3651)
                     /locus_tag="ACG8KN_002109"
     CDS             complement(2539..3651)
                     /locus_tag="ACG8KN_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9189128.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(3726..5099)
                     /locus_tag="ACG8KN_002110"
     CDS             complement(3726..5099)
                     /locus_tag="ACG8KN_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9189129.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5320..5937
                     /locus_tag="ACG8KN_002111"
     CDS             5320..5937
                     /locus_tag="ACG8KN_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9189130.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6035..6490
                     /gene="argR"
                     /locus_tag="ACG8KN_002112"
     CDS             6035..6490
                     /gene="argR"
                     /locus_tag="ACG8KN_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9189131.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            6508..8010
                     /locus_tag="ACG8KN_002113"
     CDS             6508..8010
                     /locus_tag="ACG8KN_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFP9189132.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            8105..8311
                     /locus_tag="ACG8KN_002114"
     CDS             8105..8311
                     /locus_tag="ACG8KN_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189133.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            8397..8798
                     /locus_tag="ACG8KN_002115"
     CDS             8397..8798
                     /locus_tag="ACG8KN_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9189134.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
BASE COUNT         3050 a         1657 c         1847 g         2302 t
ORIGIN      
        1 ataaagttaa tagcgtcgaa agaataaaaa acggagaaaa aaaggagttt aatatgaatt
       61 catttaagaa aattgtttta ggtacaactt ttgctgctgg tgctactgca atgtttgtag
      121 gaactacaaa tgctcacgct gatgaagttt atacagtaaa atcaggtgat tcattatcta
      181 agatctcaca aaaatttgca ggagacaact caatgatcga tgcaattgct gaaaaaaatt
      241 cgatcgcaaa catcaaccgc atttatgttg gtgaacaatt aacaatccca acttcaaatg
      301 attcttcagc aacaacagaa aacaaaacaa cagaaaatac agcatctaca actgaaacag
      361 caacgcaaga acatacatac gttgcacctg ttgaaacagt tgaagtagct cctgctgccc
      421 cagctgctgc aacagcgcca acttcaagct ctgcaaaaga atggatcgca caaaaagaat
      481 caggcggttc atacacagct actaacggac gttacatcgg ccgttatcaa ttagatgctt
      541 catacttaaa cggcgactat tcagctgcca atcaagaacg tgtagcagaa caatatgtaa
      601 catctcgtta tggttcatgg gatgctgcta aaacattctg gttagcaaac ggctggtact
      661 aagccgataa aaggaaaatg atgaaaccga gaaacttgtt tattcaagtt ctcggttttt
      721 ttgtgtgaaa aataactcgt ttgcttccca cagcgcagca gtacaaataa aaactcgaat
      781 aatgtataat aaagaaaacg tttttttgtt tagggaatga ggaggagcca tgagtacaaa
      841 agagagtctc gaattaaacg aaatcacaag tggaaatcca ctgttatcca gtattcgatc
      901 gattgtagaa acgacatttt acggtaataa tgttcatgaa gtttttgata ggaagacagc
      961 ttatcaacta gcaaaaggga gtccaggaac gatcatcaca gatttaacaa tctcacatgc
     1021 tgaagaacta tatttaccag ctgatgttag gacactagta ttcaatgatg gaagcattgt
     1081 gggaagaaca gcaagtgctc ggcggatctt tgaggactta gacaaggaac aaagcaaata
     1141 cgagaaaatt ttaagagaag ctgtgtatca aagtcgcaag cgccagtttt accatacaaa
     1201 agtaatcgtt ggcctatctg aagaattttc tgtgcaaagt catttgctga ttccagaagg
     1261 atttgagaac aatttgtatt cttatttgct gaattttcag ccagcgaatt cgaagatctt
     1321 agagacttat caaaagtctg tttcctttga tgagggagat ctttatatct tttgtgatcc
     1381 cgaatggaca catgaaacat ttccaaatgg actcattttg tttgatgctc tccataatac
     1441 agcggctgtg ctaggattac gttactttgg agaattaaaa aaagcgactt tgacgcttgc
     1501 ttgggcattg ggaaatcgca atggctttat tgcctgccac ggtgggatga aacaatatgc
     1561 tagtaatgac cagacctata cgatggctgt atttgggttg tccggttctg gaaaatcaac
     1621 gattacctta gcaaaacata cggaaggtca ctctgtttcg atcttgcatg acgatgcttt
     1681 cgttatttct cgaaaaaatg gttttgctac tgccttggaa ccttcttact ttgataaaac
     1741 acaagactat ccgtctgatg atccggccat ttcttctttc cttacttgtc agaatgtggg
     1801 cgtgacatgt acaagtaaag gagaaaaagt cctagtaaca ggagatatac gaaacggaaa
     1861 tgggcggaca gtcaaatcac gttatgcgac acccgaccga gtcgatcatc ttagcgaaaa
     1921 gatcgatgct gttttttgga tcatgaaaga tgattcgctt ccgccaatta tcaagatcaa
     1981 tgacccagtt ttagcagcgt cttttggtgc aacattagca acaaaacgat cagacgcgga
     2041 aaatatcact acaaaaagac agctagatga gctagtcatt gaaccatttg ctaatccttt
     2101 ccgtgtctat cctcttaggg aagactatca agcttttcga gagttgtttt tgaaacagca
     2161 aacagcctgc tatgtcctga atactggata tttcgatgat aaaaaaatca aacctgaaac
     2221 gacattgtca atcgttgaaa gaatcgtgca acaaacggca gaattcaagt cgtttggtcc
     2281 atttgaagac atccactatt tacctatacc agattttcct gtttcttttg aggatacgtc
     2341 ctataaaaat ctggtgaaac aacgacttga tattcgttta cgattcattg aagctcagat
     2401 aggaaaagat ctcttgccag atgaagcaaa agaaagtttg gagaatttga ttcagaaatt
     2461 gaactaaaat aacagctttt tcaagaagta atccatgcgc aatatcgctc gggatcctta
     2521 cttgaaaaag ctgtttttct atgatggatt tttcgtaata taggtggaga tgtccttttg
     2581 ataatcttcc gtaggccaat gaccagtatt taagaagttg gctaatagat agccgatata
     2641 aggacctgtg gtcaagccag aagagcctag accgctagca gccagtaagc ttggatcatc
     2701 tggcagtacg ccgaaaaatg gggaaaaatc tgacgtataa gcacgggtac ctacacggaa
     2761 ctcgtatggc cagtttttta tctgatctgg atcagtcaag aactgttctg tactcttagt
     2821 caatccctta taagcttcgc tagtaggagc caaatcccag ccttgatcgt tttcatgggt
     2881 agcacctaaa aggatctttc catccatgaa aggaatgaaa tcagcttctc cgtcaagcat
     2941 agcaacaggc cagtcttgac tcatcttata agatgtctga aatgctaaga gctgcccttt
     3001 ttgcggacgt atatctgcag catagcctaa ttcttgtaat aaaggcttaa gtgccgggcc
     3061 aggtgtcaaa attacacgat cagctgtgta ttctccaaac ggtcccttga tgagccaaga
     3121 atcgctcttc ttatgcaaag tgacttttgc ctggatctgc tccatttgtt tttgtgctaa
     3181 acgctgacca agatgttcta aatatgcttt tccatccaga cgaccaccac cactgatacg
     3241 cagtgaaact gctggtttca ataacggaag tgcttgagat gtctgttcag gtgaaagcca
     3301 ttcgatgttt ccaatttctg gcgcttcttc ttttcgtttc tcggcaagaa cagccaattc
     3361 agctaaatct ttttcaggtc gaagcaaaag tgtccctgat tgtgcgtaaa tcgtttgatc
     3421 aagttggaaa tcttttataa gtttagggaa aaaagcagcg ccgtctttag ccagacgata
     3481 ccattgtttg tttctgcgtt tcgacaacca aggggagata atcccagcgc tagctttagt
     3541 tgcctgtcct gtcccttcat caaataaagc aacattgaat tttgatgtat ctaagtaact
     3601 ggctagggtc ataccaatga tcccgccacc gataatggca attttttcca taaaaaagcc
     3661 tccttttaca aacaaccatt ttcacacaaa aaaacggcag atgcaacacc tgccgtatat
     3721 ggaatctacg cttttttagc aaagaacaga taataggcga atcgagcaaa gcttacgaga
     3781 agcaacagca acaagacgcc taatgctgct tttgatccta atgtagtaga cacttcatag
     3841 ttatgggaag tatgatgctg gacgtaattg ataatgaccg ctactaatga agtagccaca
     3901 gcgccagaaa actgctgcaa ggtattgaac attgcgttcc cgtctccttg tagttcttca
     3961 ttcagtgcat tcattccggt ggtcatcata ttgctgtaag ataatccgat accgatcata
     4021 taaaacacat gacaagcaat caatacaccc actgaagcgt gtcctatgag taggaataat
     4081 gctagccaac caatcaaaga aagaatcagc ccagcaaaaa ttggcttctt cggaccatat
     4141 tgatccagca ttttcccgga aaaaggagaa agcaaagcac caaccgctgc gccgggaagc
     4201 atcgctaatc cagcgacaaa aggactactt ccttgaacga tctgaaggaa attcggcaag
     4261 acaaaagata caccaagtaa taaaaactga taaactaaga agccaatcaa aaacaagcgg
     4321 aatgttttat ttgaaagaat agacagtcgg atcagtggat gctccacttt tttacttcga
     4381 tagtaaaaaa ggaaccagcc aataccgccg atgattaaag caatccagct ggaaactgtt
     4441 cccaacgtac tcaaaaatgt tagacttcca ctaaacaaaa gacaaattcc taaaacgctt
     4501 atccaatcca aataaccact tttttgtact gccatctcgg gaatagcaaa ccagccgata
     4561 atcaaagaaa gaaacaaaac gggcacaaga aacaggaaga tatgattcca tgtaaaatgt
     4621 gaagtcataa tgcctccata agtggggcca attgctggag caatggccgt tgtcaaagta
     4681 ccgacaccca tcatcgcgcc tcgtcgattg aggggggcaa atgtcaagat aatatggaac
     4741 atcaacggaa gagcgatacc tgtcgctgca ccttggagca atcgaccaaa caataggacc
     4801 atgaaggtag gagagaagaa atcgatcatt atacctgcca agaaaagcag atttgaagct
     4861 aaaaagagtt ttttgagact aaaattgcga ttcaaataag tagacagcgg aacaatgatc
     4921 gaaatcatca aaagataaat cgttgttacc cattggactt gtgcagtcgt taaatcaaac
     4981 tgtctgataa gagttgggaa agttacattc atggccgtct cgatcaatac gcccgcaaaa
     5041 gacatcatcc ctgttgcaat tactgcaggt actaaaagca gctgtttttg ttttgtcatt
     5101 tacatcacct ctaaaaaatt tcgtaaaaaa agagagtgat ttctattata aaaatagaaa
     5161 ccgcctctct ctcgacacgc cgaaacgtgt tatctgtacg atttatttac ttcacttatt
     5221 ctagcacaaa ctgaaaaata gtgtcaacac gattagccaa gtcactcaaa aagaggtatg
     5281 ataataaata actaaatgga atttcgcgga ggaagaaaaa tggaaaaaat tgaatggcgt
     5341 aaagataaga gctatagtat gaaaaaaagc ccgcaaatac tagaggttcc taaaaaatca
     5401 ttctttagca tcagagggat cggcgacccg aatcaggaag actttaaacg aagagtagcc
     5461 tgtctatatg cagtcagcta tgcaatacgc atgtcgccta aaaaaaattg gctgattcct
     5521 gattatcaga tgtatactgt ctatccactg gaaggacaat ggggactgca agaaaagtat
     5581 ttgcatgaac cagtgatgaa aaaagaacat ttttcttatc aattgatgat ccgacagcct
     5641 gactttgtta cagaagcgat agcacaagaa gccattcagc gttctagcaa acttccggaa
     5701 gacttgaaag aacaagtagt attcaaaaag atggaagaag gtctttgtgc ccaaatctta
     5761 cacgtcgggc cctatgccga agaaccagca acgtttgaga aattagaagc attcatcgca
     5821 aaagaaggct atgaaagagc ttctaaagaa cacaaagaga tttacatcag cgatccaaga
     5881 aaatcagcac ctgaaaaaat gaagacgatt ttgcgtgtta aaatagaaaa aggttagcaa
     5941 cctgaatgaa aacaaaaaat aaaagtaaaa aataccaaaa tatgaagaaa tattacttat
     6001 cttttgctat aatcaacatt gagaggtgtc taatatgaaa aagaaacagc gacaagcact
     6061 gatccgccag atcattacag aacaacctat tgggacacag gaagagctgc ttgcgcggct
     6121 gcatgaagca ggcgccgatg tgactcaagc aacgatttct cgagacattc gagaaatgaa
     6181 gttagtaaaa agtcaaaatg aaaacaaaat agtacgctat acattattta accagccatc
     6241 tgtttcttta aatgaagaac gtttgcgtac agcaattaga cgcgaggttt tgcgtatcca
     6301 gtctgttcag ttcatggtta tcgtattgac tgaacgaaat ggtgccgatg tggtaacgaa
     6361 ttggctagat gaagtggctt accctgaagt tgtcggaacg atggccggtg tagatacatt
     6421 cattattatt tgccgctcag aagaagaagc acagcgattt gcagaaaagc tggaaaaaat
     6481 gagggaataa atgaggagga cttttatatg aaagatatga aagcgctgca aaatggatcg
     6541 gatattcgag gaatcgcatt accagttgaa ggatatgaag taaacttgac gacagacatg
     6601 gtaaaaaaga tcggctgggg actagcaaac tggataaaga aaaagaagaa atcaaccgat
     6661 ccaaataaaa aatggacgat tgggattggt catgacagta ggataagcgg accagcactg
     6721 aaagaggcgt tgatccaaag tctgaaagaa caagggatac atgtcatcga tttcgaatta
     6781 gctactacgc ctgctatgtt tatgtccact caatttgaac agttctcgtg cgatgctgcg
     6841 gttatgctga ctgctagcca tcttccctat tattataatg gaataaaatt ctttacctca
     6901 gaaggaggag cagaaaaaga agatattgct tttatcctat cacagcagca aaaaataaaa
     6961 agcgaacaaa ctggaagcgt agaaaaagca gaccttttga caccttatgc gcaagattta
     7021 gtcacaaaaa ttaaaaaggg gattggaaca ctagaaagca aaccactaaa tggttggaaa
     7081 atcattgttg atgccggaaa tggtgctggt ggattctttg ctgaaaaagt attgaatagc
     7141 ttaggtgcgg attcaacagg ttcccaattt ttggaaccag atggccgatt tcctaatcat
     7201 atcccaaatc ctgataataa agaagcgatg gaaagtatca agcaggctgt tttagcaaat
     7261 gaagcagatc ttgggatcat tttcgataca gatgtggatc gttctgcagt tgtcagtgct
     7321 tctggaacag tgatcaatcg aaataatttg atcgcagtat taagtacaat cgtattaaaa
     7381 gagcatccag gaacaagaat cgtgacgaat tcccctactt cttctcatct acaaagattc
     7441 attgaagaaa aaggaggcat ccaagttcgt tatatttccg gctatcgaaa tgtaatcaat
     7501 aaagcattag aacttaaccg tcttgggaaa gatacacagc tagccatcga aacaagcgga
     7561 cacgctgcat tcaaggaaaa ctattttctg gatgatggtg catatgtcat cgcaaagatc
     7621 ctgatgcttt tgccagaatt aaaaagaaaa ggtatcacat tagatgaaca aatcaaagag
     7681 ttgaaacagc ccgtagaagt acaagaaatc cgattaaaat tgttcagcga tgaaccacgc
     7741 gaacaaggaa acaagatatt agaagaaatg aagcgactgt tggaagaaga aaaagggatg
     7801 aacttcgacc cggaaaatga agaaggtatt cgatatgatc tagaagagcc atacggaaac
     7861 ggctggtttt tgatccgttt aagcttacat gagccgttac tagtattaca gatagaaaat
     7921 gatgaattcg gaaagaacaa gttggcgtta gtaaaattag caaaattatt cggacatttt
     7981 tctgatcttg aatcattaac agcagaataa accagtcttt ttgaaaggct agttgcttgg
     8041 acggcaaaaa aagtggtatg gttaaatcga gtgctggtag ggagaccagc aaaaggagga
     8101 aaaaatggta acgaatatgg ttattttttt aacagtattg gtttatgggg tcaactttct
     8161 ggtatatatg tatcttaaaa tcaatcatat aaaagattat gtagaaaaga ttgctattta
     8221 ttttggaaca aatatgacgc tgttgttcac gagtagtatt tttttgtttt ttggaaaaat
     8281 agtagaagac ggcattctgg tattcgaata agcagaattt ttgtagaaag cgccttactt
     8341 gtcatacact ataattatag ccaagtagca aatggtcaat cgaaaggagc agtacaatga
     8401 aaaaagcata cgaaacagca atgatcaacc atggcgggcg caatggagaa gtcgaggcac
     8461 caaatggctc gatgcatatg aaaatcactc ctccaggaat ccatgcagaa ggaacaaatc
     8521 cagaacaatt atttgcagca ggttatgctt cttgtttcaa tggggcggta caacatatga
     8581 tcaaagaagc aggaatgaca gcagagtcca cagtaaaagc acgtgtctct ctttataatt
     8641 tagatgatgg cggctatcag atcggtgtag tattagaagt gcatatcgat ggcatcagcg
     8701 aagaagaagc ccaaaaaatt gcagaagatg cgcatgaatt ctgcccttac tccaaagcaa
     8761 ctagaggaaa tatcgatgta gaagtaacga ttgtttagaa gaataaagga aatcgaacca
     8821 gactaaaaac gtgaaaggac cgctttttgt ctggtt
//
LOCUS       DAZKCZ010000094         8744 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000094 DAZKCZ010000000
VERSION     DAZKCZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.095"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..299
                     /locus_tag="ACG8KN_002116"
     CDS             <1..299
                     /locus_tag="ACG8KN_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189135.1"
                     /translation="DYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPV
                     RTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(396..830)
                     /locus_tag="ACG8KN_002117"
     CDS             complement(396..830)
                     /locus_tag="ACG8KN_002117"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9189136.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1062..1772)
                     /locus_tag="ACG8KN_002118"
     CDS             complement(1062..1772)
                     /locus_tag="ACG8KN_002118"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9189137.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1762..2622)
                     /locus_tag="ACG8KN_002119"
     CDS             complement(1762..2622)
                     /locus_tag="ACG8KN_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP9189138.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2627..3265)
                     /locus_tag="ACG8KN_002120"
     CDS             complement(2627..3265)
                     /locus_tag="ACG8KN_002120"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9189139.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3280..3579)
                     /locus_tag="ACG8KN_002121"
     CDS             complement(3280..3579)
                     /locus_tag="ACG8KN_002121"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9189140.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(3622..5106)
                     /locus_tag="ACG8KN_002122"
     CDS             complement(3622..5106)
                     /locus_tag="ACG8KN_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9189141.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(5126..5587)
                     /locus_tag="ACG8KN_002123"
     CDS             complement(5126..5587)
                     /locus_tag="ACG8KN_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9189142.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="ACG8KN_002124"
     CDS             complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="ACG8KN_002124"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP9189143.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6890..7660
                     /locus_tag="ACG8KN_002125"
     CDS             6890..7660
                     /locus_tag="ACG8KN_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9189144.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7933..8744)
                     /locus_tag="ACG8KN_002126"
                     /pseudo
     CDS             complement(7933..8744)
                     /locus_tag="ACG8KN_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2639 a         1842 c         1383 g         2880 t
ORIGIN      
        1 tggattatac tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt
       61 tcatgtataa agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc
      121 gtataaagcc agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta
      181 ataaaaaaaa ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata
      241 ttagttcgaa tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc
      301 tgagcattgg aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact
      361 aagctagaat tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc
      421 ttttttcttc tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat
      481 cctactgatt cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg
      541 actggtccat aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct
      601 tggcgctgat acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca
      661 gtgatccaac gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca
      721 gattgttcaa atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg
      781 ctcttatata attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc
      841 ctttcttata caattctccc tctaataata cagttctttg actatttaat ctatcacata
      901 taactctcct gtaaagattc tctcgattcc ttaaaatctc tctttccttt tagaatgcat
      961 cctttttgat aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat
     1021 caagtgttat cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca
     1081 tactttccat gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata
     1141 ttggggttga tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg
     1201 ttttctactg ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt
     1261 actccaggga cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc
     1321 cctgtatgaa cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga
     1381 accttaccat aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct
     1441 tgcgcccaca tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa
     1501 gtttgataca aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct
     1561 attatatttc ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg
     1621 acaccgcttg gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc
     1681 caagtcaatt tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc
     1741 ttcattgctg caattttttc ttcacaagac atcatagcca gcctccttca atttagaact
     1801 taagtagtct tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat
     1861 ttcaatcaga aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa
     1921 atcaacacaa ccgctaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac
     1981 agggtaagta tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg
     2041 agccggatta ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa
     2101 aggggaatga atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc
     2161 atccatgatt tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt
     2221 tattccttcg ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta
     2281 tccagctatt tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat
     2341 tagttgtttc gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg
     2401 cgcacttaaa caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc
     2461 agctatcttt tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga
     2521 catctcgata aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat
     2581 atctttgggt aatgcttttt cataaatccc aatttgagcc atttccttac ccccagatac
     2641 gaatgatctc atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac
     2701 cacgaccagt gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat
     2761 ttaatccacc agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata
     2821 aatctttttc accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta
     2881 cctgccggat accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga
     2941 gttctacttg caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac
     3001 aacaaatgac tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc
     3061 cagcgtctgc acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac
     3121 agcgaatcgc tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta
     3181 ctacatcacc aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg
     3241 caatttgcaa attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca
     3301 ctttgtttca gcaaattttt cactgatttc tttggctgac atcacatttt tgataccgat
     3361 aactgtaact tcttttttct ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa
     3421 aactacatcg tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt
     3481 ggtaattggt acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct
     3541 tgttcctgat ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt
     3601 gttgtctaat cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc
     3661 tacaatcaaa aaataaccct caggattttt ccgatattgt aactgcggaa tagccaataa
     3721 accaatgaca actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa
     3781 tggccaagct gttgcccagt cgaacatccc gatataacca ccatatgttg acatcccaat
     3841 ccatgtagcg aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag
     3901 gcaagcggct ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa
     3961 gaacaacgga atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc
     4021 aatcatcaaa aactgtccca aagcaccaaa caagaaccca atcgttaccg catttggcga
     4081 accaaagcca aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaattgtgtt
     4141 tgagattcct tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa
     4201 gatcgccaga tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg
     4261 tccttcttgc atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat
     4321 cccaaagaaa aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa
     4381 gaatcctggt aatttgatat cttcgatttt tttgtcttgc ttgattgatc tatttttctt
     4441 ttcatcccgc tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg
     4501 ctgatgtgcc actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa
     4561 gttagaagct actgcccaat aacaacctaa aatcagtccc ataaaaatca atacttgtat
     4621 cctacctaga tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac
     4681 ctgaacgttt ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa
     4741 taagatattt aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc
     4801 aaatgtctcc attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat
     4861 ggcatccaaa ttgaaccgtt cctttagtcc aaccaaaatc ggccgaaaat tattgaccaa
     4921 accaccagag ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat
     4981 gcattcatga agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag
     5041 gtatgctggc tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc
     5101 ccccatcttt tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat
     5161 cgatggagtt cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata
     5221 atttttggat attttctaga tgttcttctg gatcttttgc tgcaagtgta aaaaacaaac
     5281 tagccgtttt ctcctcatcc tccccttcac tttcaaaacg gattggctgt ttcaacttgg
     5341 taaatgaaat agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta
     5401 cttgaggtgc gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca
     5461 catacgtttc atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt
     5521 cacgccaatc cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt
     5581 tcagcaagaa actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat
     5641 cgagaagaca tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg
     5701 atagatgaat ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc
     5761 ctttttgaat ttccaaattt cagtgatctc ttttgaatca gccataaaat tagaccaaat
     5821 atcgtagtgg acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc
     5881 aacagaagta actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac
     5941 atcgattttg tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc
     6001 atgataaaga ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc
     6061 ctgaggcatt tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg
     6121 atcaaacgct tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt
     6181 tactgtaatc gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt
     6241 tggcccaatg aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc
     6301 tagatgatcg gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg
     6361 atcgattacg aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg
     6421 catcatctga tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca
     6481 ccatagatca cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc
     6541 cagccaccac atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa
     6601 gtatgtgccc cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac
     6661 agttttactc atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa
     6721 tgtgtatcag catccatgga aaccctttta tatgaccgtt tgttttttat atgattttct
     6781 ttcagtaatc aatgattgtt tcagtataat aaagacagat atgaatgttt aaatgaacaa
     6841 ctagtcaaat aaatccatgg atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc
     6901 acttgtcaat attgaaaaac gtcaaaaaga aattttggaa ctgcttcagc agactgaaca
     6961 actatcaact ttcgaattag ctgaatccct tgatgtatct attagtacga tacgaagaga
     7021 tttaaattta ttagaagaaa aaaacgagat catacgcaat tacggttatt gttcttataa
     7081 ttacaaaaac cagactgatt ttgatcaatc tggtcctgaa aggattaaac aagcgattgc
     7141 cagagaagct agtaaataca ttaacgatta tgatacgctg tttatcaatt ctagctcgac
     7201 tgcgttgaaa acactggatt ttcttcaatc taaccatttg acagtcatta caaacaacgt
     7261 gaaaatcaat tactctccgc acaaagataa ttgtagttat attttaactg gtggagagat
     7321 gcgctttcca aaagaagcac tagtgggcga catcgcatta aatacgattt cttcagtcaa
     7381 tgcagacatt tgtatcattg gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa
     7441 gatattaaat gaatcaacga tcaacgaaat gatggtcaac aagacaatca aatgtaagat
     7501 tttagtcgct gatcatcgaa aaatcggact gacatcaaag ttcaagattg ctgatatttc
     7561 agtctttgat tatttgatca ctgataaatt ttgttctgaa aaagtattag aggagatcag
     7621 aaatacgggg ataaaaacca ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt
     7681 aaataggatt atcaaaaaaa tgaataacaa atttatcgat aaatgaaaaa cacgctacgc
     7741 tcaaattgat aaaggaatcc caagatgtgt ccatccaatc gataccatca taatctattg
     7801 cttcttttat aaccgttata tgttgcctaa ataggaatca aacaatatcg aaaaaaggag
     7861 gtatgacaaa agtcacacct ccttttttcg aataaacggt attcaaatca gaacactgat
     7921 ttcacaacca ctttatttca ggtcgttttc aatcaccttt accgcgtaat catttccgtt
     7981 gaaacgcgct attttttttg cgtccatata tctttctaaa taatcatctt ctcctaattg
     8041 cttatggcaa gaagcaattt cataaagcaa gtattccaat gcataagtcg atttatgttt
     8101 cttattatac tcaataccct tctcacataa ttggattgct ttatcataat cctcgatttt
     8161 acggtaatag gtggcgatat cataatatac aatgcagacc atcagattat caaaatcagt
     8221 ctcactcaat attttaagtg tacgctccac ataaactttc gcaaaatcta attctccttt
     8281 tgacaagtag atcattgcca cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt
     8341 cgtttgttcc aacacttgat taaaataaaa taatgctgca tctggttttt ttcgcataaa
     8401 gatgatcaag cagtccttga taaaataaaa tttgatttaa aagatcagaa ttacttactt
     8461 tttcctgatc gatcgtttgt aaaattttat aagattcttg gtattttgat acagaattca
     8521 atgattgaat ctcatttatt tttttcctga ttttgtcttc ttctgtttct actaatatat
     8581 cggagatctc gacattcagc tttttgcaaa gcgcacttaa gatattgatg cctggcgaca
     8641 tattcttgtt ttcgatattg ctgacagttg cttgttcaca aatgccttct gctaattgta
     8701 cttgtgttaa cccctttttc tttcggtatt cttttagtaa tgct
//
LOCUS       DAZKCZ010000095         8671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000095 DAZKCZ010000000
VERSION     DAZKCZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.096"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..504)
                     /locus_tag="ACG8KN_002127"
     CDS             complement(58..504)
                     /locus_tag="ACG8KN_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFP9189145.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(531..707)
                     /gene="rpsU"
                     /locus_tag="ACG8KN_002128"
     CDS             complement(531..707)
                     /gene="rpsU"
                     /locus_tag="ACG8KN_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9189146.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(869..1297)
                     /locus_tag="ACG8KN_002129"
     CDS             complement(869..1297)
                     /locus_tag="ACG8KN_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9189147.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1389..2249
                     /locus_tag="ACG8KN_002130"
     CDS             1389..2249
                     /locus_tag="ACG8KN_002130"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFP9189148.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(2341..2691)
                     /locus_tag="ACG8KN_002131"
     CDS             complement(2341..2691)
                     /locus_tag="ACG8KN_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9189149.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(2706..4433)
                     /locus_tag="ACG8KN_002132"
     CDS             complement(2706..4433)
                     /locus_tag="ACG8KN_002132"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFP9189150.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(4647..5573)
                     /gene="trxB"
                     /locus_tag="ACG8KN_002133"
     CDS             complement(4647..5573)
                     /gene="trxB"
                     /locus_tag="ACG8KN_002133"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9189151.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(5718..7151)
                     /locus_tag="ACG8KN_002134"
     CDS             complement(5718..7151)
                     /locus_tag="ACG8KN_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9189152.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(7151..8554)
                     /locus_tag="ACG8KN_002135"
     CDS             complement(7151..8554)
                     /locus_tag="ACG8KN_002135"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9189153.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALNDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         2359 a         1941 c         1471 g         2900 t
ORIGIN      
        1 ttttttacga acacaaaaaa ctgcggacaa tcactgtccg cagtttaaat gataaaatta
       61 agattgtaat agttctttca caatcgcatt cacttggttt ccatctgctt ttcctttgac
      121 tttaggcata attgcaccca taactttgcc aaattctttc attgatgaag caccagtttg
      181 agtgattgct tcttgaacaa gttgtcgaat ttcttcatcg gaaagttgtt ccggcatata
      241 tttttcgact attacaattt cactttttac tttttcagct aaatcgtcac gtcctgcttc
      301 ttcaaactca tgcaaagaat cacgtcgctg tttcatttcg cgagacaaca ccgttagttc
      361 ttcttcttct gtcaagtcgt gaccttcttt aatctgctcg ttttgtatag atgacttgat
      421 cattcggatg acttgtaaag attccttatc tttcgctttc atcgctgctt tcatgtcatc
      481 gtttaatcta cttagtagtg acacactacc aactccaatt catcacagaa ctagaatttt
      541 ttgcgttttc tagctgcttc agattttttc ttgcgtctta cacttggttt ttcgtagaat
      601 tcacgtttac gagattcttg taaagtaccc gcttttgaca cggaacgttt gaagcgacga
      661 agagcatcgt caagtgattc gtttttgcga acgactgttt ttgacatgtt aattccctcc
      721 ctccgagctt aaaaagtaac cgtaattgtt attgaaatat atctcaaaaa acaagactgt
      781 cctgtaaata ttatatctaa tgctgttttg accgtcaagg aaatcttgct tttttttaaa
      841 attatcaaat gttattttat ttaaaccctt attgacaatt ttcacatctt ccgaaaatct
      901 cgaatctatg cccttcgatc gtgcatccac ctagctgttc ttcgaaataa ttcatcggac
      961 acatatggat ttctttcgtt gttccgcaca ctgtacagat aaaatgatgg tgatggccga
     1021 tttccccctg acagcaatga aaacgaaact tcatttctcc atttaaatcg gtttcttcca
     1081 gtaaatcgat ctctgcaaaa tcatggagat tccgataaat cgtatcataa cttacccctt
     1141 tgaattcctg attcatatag tcaaaaactt ctttagcaga tacgtagcgg ttacgcttca
     1201 ctaaataagt taacaatgct tctctttttt tagtatattt aaaaccgtta tttttcaatg
     1261 tttctaatga ttgttcgatc aatgtctgtt ttttcatgac ttattcctcc aaatcaaaat
     1321 gattacgaag tacgcattct agagtatggt ataataaaca taaaaaaaat gcaagtgagg
     1381 ttttatccat gtcttccgat tctacagaaa tcgttacttt ttttgtcatc tccgactctg
     1441 ctggagaaac tgcgacaaaa ctagcgcaag ctactatggc tcagtatcct accgttgagt
     1501 tcaacctatt ccgcagaacg ttcgtgacag atgaaaaaac attaaagcag gcattgcaag
     1561 atgcattaga agaaaaagca cttgtcctgc atacattaat caatcaagaa ctgatcgaga
     1621 taacacgttc tttttgtgaa gaacatcaat tatttagttt tgatgtgatg acgccaccga
     1681 tatcagaaat tgaacgttta accggtatca agccaattcg gcaacctggt gccctccatt
     1741 tattgaatga gaactatttc aaacggatca aagcaatgga atttgctgta aaatacgatg
     1801 atgggaaaga tcctcgtggt tttttagaag cagatgtcgt tttgctaggc gtatcccgaa
     1861 cttccaaaac gcctttgagc ttgtttttag ctaacaaaaa tttaaaagtt gccaatttac
     1921 ctttgatccc acaagcacat attcccaaac agctttggga gatcgatcct aaaaaaatcg
     1981 tgggactaac gaataaccca gatattttaa ataatatccg taaagaacgg atgcgctctt
     2041 acggattaaa ccctgatact gcttattccg atatcgaaaa aattagagct gaactcgatt
     2101 ttgcgaatga tctttatgaa aaactaggat gtgtagtgat caatgtggca tctttatcta
     2161 ttgaagaaac ggcttcgctt attttgaatg ctttagacct agaagatcat agttattatg
     2221 gaactgaaac aacagaaaaa agcgattaat catttttgtc tttttcttaa aaaacactgg
     2281 agtgtgacaa cgaagaaatt tcgttgtcac actccagtgt ttatctattt gcacctatcg
     2341 ttattgatgt tccacatgtt tgagtgtctg gttcaaaata tccagtacat gagaatcatc
     2401 aagactatac aaaatcgctt ttccctcgcg atgagacttg acgacatgat tttctcttaa
     2461 taaacgtagt tgatgcgata cagcagactg ctccatttcc actgcttggg tgattgctgt
     2521 cacattttgt tccccgtctt tcagatatag cagaatttta agacgagtgg gatcactcaa
     2581 cattttgaat aattgactga ctttctctac ttttgatttt tgcgtgtctg gcaatccatt
     2641 cacctccaac ttgaaaaaag actgacttct aaagtttacc actttttcca tcagtctcaa
     2701 gttttttatt cttctgtcat ctttttgata atcgcttcat aagaggctac tttttcggct
     2761 gcgtcttctg tagagcttcc ttttacacca atataaaact tgattttcgg ctcagttcct
     2821 gaagggcgga tagcaatcca gccattttct tccaatgtat atttcaatac gtcggatggc
     2881 ggtgttgtta gtttttctac gtttccttca cggtctgttc tcgtcaattc tttaaagtct
     2941 tctgtttgaa taacttttgc tccggcaaat tctgtcggtg cttctttacg gaatttttcc
     3001 atcaaagcag agattttcgc agaaccttct tgtccgctca ttgtcacaga aatcgttttt
     3061 tctgcaaaat agccataggt ttcaaaaatc gtttgcaagc cgtcatacaa cgttttgcct
     3121 tgttttttat agtaagctgc tacttctgct aataaaagca atgcttggat cgcgtcttta
     3181 tcgcgaacaa aaggtttaac taaataacca taactttctt cgaatccaaa cataaaagta
     3241 tgtgaatggt cttcttcgaa ttgttggatt ttttcagcaa tgaatttaaa ccctgtcaac
     3301 acattgaaca tggtagcacc gtagtgttcc gcaattgctg taggcaattc gctggaaacg
     3361 attgatttca ataccgctgc gttttctggt aaagtgcctc ttgattgatg tgcttccaaa
     3421 atatattgag caaacaatgc cccgatttgg ttcccagtca atacttcata agaaccatct
     3481 ggcaaacgaa ccgctgcgcc taaacggtct gcatcaggat ctgttgcaat cagcaagtca
     3541 gctccttctt tttctccaag tcgtatagca tattcaaaag ctgaatgttc ttctggattt
     3601 ggcgatttga ccgtagtgaa atctggatct gccacagctt gttcgggaac tagtacgaat
     3661 tgttcgaatc cagcttgttt caacgcacgt tcgcccaaca ttttccctgt tccatgaagt
     3721 ggcgtgtaca caagtttcaa atctttcccc attgtttcta ccaattcacg atcaatcgtc
     3781 actgctttga tttcttctag ataagcagcg tccacttctt ctccgatgat cgtgatcaac
     3841 ccgctatgtt tagcttcttc gtcagataat acttcgactt ttagcggatt gctgacttca
     3901 cggacatatt tcgtcaatgc atctgcgtct gcaggaggca tttgtccgcc atcttctccg
     3961 tagactttat atccattgta agccgctggg ttgtgagatg cagttaccat gattcccgca
     4021 aacgtcttca aatgacgaac tgcaaaagaa agctctggtg ttggacgcag actttcgaat
     4081 acataagacg ggatattgtg atgtgccagt gttttggctg cttccattgc aaattctggt
     4141 gactgatggc gtgagtcata agcaatagcg acacctcggc gttttgcttc ttcaccttgt
     4201 gtatccatga aacgtgctag tccttctgtt gcttgtctta ccgtatagat gttcattcga
     4261 ttgatcccag gtcctaagat tccccgcatg cctgcagtac cgaattccaa cggtgcataa
     4321 aaagcatctt caagtttttc aggatcttca cccagatcat ttaattgttt ttttaagttt
     4381 tcttctaaat tttcttcatg tacccattgt tcatacactt gttcccaaga catgaaaata
     4441 acacctcttt ctgaaatatg gttcttaata agtatagcat tcacttatta gatatgaaag
     4501 tatgaggacc aaaaaacgct taaatcaaga aaggattctt ttatcaaaag gtgttggacg
     4561 tgttcgcata aatcagattc agtaaatttc acgcgaaaaa aaagagtaaa agaacaattg
     4621 aacccgttct tctactcttt ttaaacttat gcagttgttt cttctaaaga ttccaagtat
     4681 ttatacactt gttgtccagc aataccgccg tcaccaacag ctgtagtgat ttgtcgcaaa
     4741 tctttttcac gaacatctcc gatggcaaaa acgcctggca tattcgtttt catttcgttg
     4801 tctgttggaa tccagccagc ttcgttcgtc aaacctgctt ctctaaaagg tgcagttaat
     4861 ggatccagtc caacatagat gaatactccg tctgctgcta aatcaaattc attgccggtt
     4921 ttgacattac gtgctttcac acctgtcact accatttcgt ttccaacgat ttcgtcaacg
     4981 actgtatccc acataaagga aattttttca tttgcaaatg cacggtcttg gatgatcttt
     5041 tgtgcacgta attcatctct acggtggacg atcactactt cagaagcaaa ttgtgtcaaa
     5101 tagatggctt cttctactgc tgagtcccca cccccgatga ctactagacg tttgttacgg
     5161 aaaaacgcac catcacagac tgcacagtaa gaaacgcctc gtccagcaaa ttcttcctcg
     5221 ccaggtacgc ctaatttacg gtggatacag cctgttgcga taatgactgt ttttccttcg
     5281 taacttttat cttcacagat gacttcttta tggctgccgt gatccttgat atcttgaacg
     5341 attccataag cattttctgt cccaaatctt gagacacttt catacatctt ataagctaga
     5401 tcggggccca tgatcgaatc gaatccagga taattttcga tttctgccgt attgttcatt
     5461 tggccgccag gggcaccttg ttcgatcatc aatacggata agtttgaacg tgaagcatat
     5521 aaagcagcag tcatcccagc aggacctgcg ccaataacga ttacatcata catatgtaga
     5581 ttctccttta cttaatcaag taagcatact ttacagccaa tgcgaaaaaa agtctattgt
     5641 tctgcttgcg tttctcgaat agctttatta tactcgaaaa atccgctgaa aaacaataga
     5701 cttttataaa tcattgatca atcgttcgaa agattttctc catttgaatc aattactttt
     5761 ttataccagt tgaatgattt tttctttgaa cgggctaatg tgccatttcc ttggttatca
     5821 cgatcgacat aaatgaatcc gtatcttttc ttcatctcac cggtaccagc tgatactaaa
     5881 tcaatacatc cccaagtcgt atagcctaat agatcgactc cgtctagttc tactgcatcc
     5941 ttcatcgcct ggatatgcgc tgccaggtaa tcgattcggt aatcatcttc cacataacca
     6001 ttctcatctg gcgtatcgac tgcacctaag ccgttttcta caataaataa tggtttttga
     6061 taacgatcat aaatatcatt catcgttatt ctcaagccta atggatcaat ttgccacccc
     6121 cattcacttg cttgcagata tggatttttg acagaagcaa agatatttcc tgcagtctgt
     6181 tccaagagtt cgttgtcggt agtcgaaact cgggaagaat aataagaaaa agagataaaa
     6241 tcaacagtat gttctttcag caatgcggca tcccctggtt ccatttcgat ctgaatgcca
     6301 tttcgttcca tttctttcaa tgcataagct gggtattctc ctcgtgactg aacatcaatg
     6361 aagaaataat tttctcgatc tgattttcta gaagcccaaa catcttcagg attgcaagtg
     6421 tatggataat atgctcctgc tgccagcata cagcctactt tgttctccgg atctacttca
     6481 tgagcaatct tcgttgcgat ggcacttgca accaactcat gatgtgccgc ttgatatttg
     6541 acttgttctt cgttttctcc ttcttcaaag tacagtcctg ctcccatgaa tggtgcatga
     6601 aggatcatat tgatctcatt gaatgtcagc cagtatttga caagtccttt atatcgattg
     6661 aagatgactc gacacagatt ttcataaaaa ccaacgagtt ctctggaacg ccacgcaccg
     6721 tattctttga tcaaatgcat tggacaatca aaatgcgtaa tcgtcacaag tggctcgatt
     6781 ccgtgctttt ggcattcttt aaagacatcc tcataaaatt tcaagccagc ttcattcggc
     6841 tctttttcat cccccattgg aaaaatacga ctccaggcga tggacattcg ataagtcttg
     6901 aaccccattt ctgcaaacaa agcaatatct tctttataac gatgatacat atcgattgca
     6961 atttttgctg gataaaaatg atcctcatca aattcaaaca tttttttctt tccggtgata
     7021 acaggaaaac ggtcttttcc gattggcaca acatctacgt ttgctagtcc gcgtccacct
     7081 tcatcataac cgccctcaca ttgattggct gctgtagcac cgccccataa aaaatctttt
     7141 ctgaatccca ttatacttcc tccattatat gtgtattacc tgataattct cttgccagtt
     7201 catcttgaac ttcgattcct tcttctttta atcggctgat tgtttccttg aattgcggta
     7261 aatactgctg gtgtgcaata aacaactcat ccatgatccg ttttgctgtc tttcctgatc
     7321 gtataagcgg attgatcgta aacgcctgta aagctgtccc ataatcacca gtcacagctg
     7381 cttcgatcgt cagcaattcc atattcttca ttacttgcaa ccagcctctt tcagcagcag
     7441 gaagagaacc aaacgctaca tttctcgctc cttgtgcgcc aacataggca gtgacttcta
     7501 ctacacaatc tgccggcagg tcaggaacag ctccatcatt ctttgtagat accacgatct
     7561 gtgtttcttt atttccatag attgaagcaa tcgtttcaca ggcagcgtct gaataataag
     7621 cgccacctcg tttttgcaat tgttcaggtt tatggtctaa ttctggatct ttgtataatt
     7681 caaatagctc tgcttctgtt tctttcactt gctgcgctct tgtaccaatc gtttgatatt
     7741 cttctaaagc atgttccagc atttcttctt cacggtagta atagcggtgg tagccacaag
     7801 ggatcatatt catttgtttt aattgttctt tgaaaaacgg gatatcaaag atatttttcg
     7861 gcaatccatt gtcttcatcg tataaacgat caatcaattc tgccgtgcgg tcatttcctt
     7921 tattatctgc tactttgtgc caatggaaat gatttagacc ggcaaacttg tagatcagct
     7981 cttctttttt cttgccaagc attgctggtt ctgtcatcat cgccattact ggcacattgc
     8041 ataatccaat cactttatcc catttgccat atctgataac tgcttctgtc accatcccac
     8101 ttgggttcgc gaaattgatc agccaagcat ttggacataa acgcttcatg tcttcaacga
     8161 ttgccaagac cactggaacg gtacgaaaag ctttgaatat cccgccagca ccatttgttt
     8221 cttgcccaat catcccataa taactaggga tccgctcgtc tttgattcgt gcatctaatt
     8281 gaccgacacg aaactgagtc gtaacaaaat ccgcatcatt caatgcttct tcccgatcca
     8341 gagttgcata gattttgact tcatacggag aagcatccca catacgctga gacatctgac
     8401 taacgatctc aagcttttct tttccttctt ctacatctac gagccagatt tctcgtatcg
     8461 gtaattcctc atatcttttg ataaatcctt ccatcaactc aggggtgtaa ctgctgcctc
     8521 caccaatcgt agcgattttg attccttttg ccatgttctc actcctttac ttttttacac
     8581 tttcattatc ttttttgtaa acaaaacaaa caatagaaat ccgtttcttt ccagtaacat
     8641 ttacaaaagt tactgttttc tagtggattt t
//
LOCUS       DAZKCZ010000096         8468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000096 DAZKCZ010000000
VERSION     DAZKCZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.097"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..2703
                     /gene="clpB"
                     /locus_tag="ACG8KN_002136"
     CDS             94..2703
                     /gene="clpB"
                     /locus_tag="ACG8KN_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9189154.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2854..3540)
                     /locus_tag="ACG8KN_002137"
     CDS             complement(2854..3540)
                     /locus_tag="ACG8KN_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189155.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3518..4012)
                     /locus_tag="ACG8KN_002138"
     CDS             complement(3518..4012)
                     /locus_tag="ACG8KN_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9189156.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4032..5252)
                     /locus_tag="ACG8KN_002139"
     CDS             complement(4032..5252)
                     /locus_tag="ACG8KN_002139"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9189157.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5399..7234
                     /gene="lepA"
                     /locus_tag="ACG8KN_002140"
     CDS             5399..7234
                     /gene="lepA"
                     /locus_tag="ACG8KN_002140"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9189158.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7328..8455)
                     /locus_tag="ACG8KN_002141"
     CDS             complement(7328..8455)
                     /locus_tag="ACG8KN_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9189159.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2721 a         1622 c         1701 g         2424 t
ORIGIN      
        1 tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag
       61 tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg
      121 cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc
      181 gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg aaatttctat
      241 acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag
      301 tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt
      361 cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg
      421 gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat
      481 aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa
      541 agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat
      601 ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc
      661 cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa
      721 cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat
      781 gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct
      841 ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa
      901 aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga
      961 aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa
     1021 ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa
     1081 gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc
     1141 agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac
     1201 aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg
     1261 gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct
     1321 atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct
     1381 gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa
     1441 ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa
     1501 gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa
     1561 gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct
     1621 caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg
     1681 gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc
     1741 cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt
     1801 cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt
     1861 tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca
     1921 actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa
     1981 gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta
     2041 gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc
     2101 agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc
     2161 ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta
     2221 gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg
     2281 gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta
     2341 ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca
     2401 ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa
     2461 cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct
     2521 gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa
     2581 gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta
     2641 acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc
     2701 taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc
     2761 cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct
     2821 tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt
     2881 ataactttca aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa
     2941 ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc
     3001 gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc
     3061 gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa
     3121 taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat
     3181 caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca
     3241 aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa
     3301 aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac
     3361 ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg
     3421 agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc
     3481 aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat
     3541 ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca
     3601 atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt
     3661 gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc
     3721 cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga
     3781 tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag
     3841 caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt
     3901 gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt
     3961 tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat
     4021 catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct
     4081 agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca
     4141 cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct
     4201 ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca
     4261 ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca
     4321 aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg
     4381 atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata
     4441 tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt
     4501 cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag
     4561 tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca
     4621 tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca
     4681 tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat
     4741 cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag
     4801 gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga
     4861 tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga
     4921 tcttgcatca atccatggtt agcatattct ggataagttc caactccagt cacaccaccc
     4981 attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc
     5041 agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa
     5101 cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg
     5161 aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat
     5221 tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata
     5281 acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa
     5341 attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat
     5401 ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca
     5461 tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac
     5521 gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat
     5581 cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat
     5641 ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct
     5701 tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac
     5761 attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa
     5821 aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg
     5881 aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat
     5941 tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa
     6001 agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat
     6061 cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt
     6121 tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt
     6181 cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga
     6241 tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa
     6301 tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga
     6361 agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca
     6421 agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca
     6481 agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta
     6541 ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga
     6601 acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa
     6661 tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat
     6721 ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt
     6781 ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga
     6841 aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa
     6901 agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat
     6961 cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc
     7021 tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc
     7081 taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg
     7141 gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt
     7201 tctgaaaatg gacgaggacg aaccgaagaa atagcaaggg tttgtttgaa aacagtgtaa
     7261 aaaataaacc atgtttacat ggatttgcca ccggtttgcc accggtggtt tttttcggtg
     7321 gcaaaaatca agaattcttt ctaagatttt ccatgatatc gaccgtttca tttctcatct
     7381 tatctgttac atgcgagtaa gtatccatcg taattgaaat tctgctgtgg cccaatcgtt
     7441 cagagatttc tttcattttt gcgccatttt cgagaagaag tgtagcatga gtatgtctga
     7501 gagaatggaa gttaaaagag agggagagtg cattcgatat cctcctagta ttccatttca
     7561 ctacacttgg cgtaactagc tcaccgtctt ccttcgtaca tactgcattt gaatcaatgt
     7621 agagctttcc atacttcatt cgattttcta attgttgttt cttatgtttt ttcagaattg
     7681 ctagcaaggt ttgtccaata aaaatcgttc gattggaact gcttgtcttt ggcgtaccat
     7741 atacccatgc gccgtcattc tttaccattt gtttctctac agtaattgtt ccatttgaaa
     7801 aatcgacatc atcccacgtc agaccacaaa cttcgccaac gcgcattccc gtataaaatc
     7861 caatattcaa aggaatatag aaaggatgtc cttcaggagt aatttctagc atatgatcaa
     7921 agtcctcaag agaaatgatt tttagatctt ttttagtcgt tggtcgtgct tcatattttg
     7981 gtatttttac atacagcata ggattttgct tgattaatcc ccaaggataa accgccatat
     8041 tcagcgcatt cttaaggaca gagtgagtaa tagtcattgt tttcttcgag taaccctttt
     8101 taaattcagc attgatgaaa ttttgtaaaa gagcagggga gagatccgta agttttttct
     8161 ttcctaaata accgtttata tgatttttga tggtaaatcg gtagttttca taggtattgt
     8221 attttagatt tagtttaacg tattcctcca tccaaaaatc aaggtattgt tttactcgag
     8281 tatccgtacc taaaaagtat tgtcctgttt cgtcaatatc tgataaaact tttcgtaaag
     8341 cagcttcggc ctctggtcgg gtgtctccgc caactttctc cacttttttt cttgagccat
     8401 catcattgat atcttcgccg tttgtataat taagcgagac aaataaaaaa gccatttatg
     8461 ttacactc
//
LOCUS       DAZKCZ010000097         8398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000097 DAZKCZ010000000
VERSION     DAZKCZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.098"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..237
                     /locus_tag="ACG8KN_002142"
     CDS             55..237
                     /locus_tag="ACG8KN_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189160.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            221..406
                     /gene="tnpB"
                     /locus_tag="ACG8KN_002143"
     CDS             221..406
                     /gene="tnpB"
                     /locus_tag="ACG8KN_002143"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9189161.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            649..2169
                     /locus_tag="ACG8KN_002144"
     CDS             649..2169
                     /locus_tag="ACG8KN_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9189162.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(2356..2928)
                     /gene="hxlB"
                     /locus_tag="ACG8KN_002145"
     CDS             complement(2356..2928)
                     /gene="hxlB"
                     /locus_tag="ACG8KN_002145"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFP9189163.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(2935..3597)
                     /gene="rpe"
                     /locus_tag="ACG8KN_002146"
     CDS             complement(2935..3597)
                     /gene="rpe"
                     /locus_tag="ACG8KN_002146"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9189164.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(3613..4128)
                     /locus_tag="ACG8KN_002147"
     CDS             complement(3613..4128)
                     /locus_tag="ACG8KN_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFP9189165.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(4130..4414)
                     /locus_tag="ACG8KN_002148"
     CDS             complement(4130..4414)
                     /locus_tag="ACG8KN_002148"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9189166.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(4448..5776)
                     /locus_tag="ACG8KN_002149"
     CDS             complement(4448..5776)
                     /locus_tag="ACG8KN_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9189167.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(5790..6245)
                     /locus_tag="ACG8KN_002150"
     CDS             complement(5790..6245)
                     /locus_tag="ACG8KN_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9189168.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(6248..8194)
                     /locus_tag="ACG8KN_002151"
     CDS             complement(6248..8194)
                     /locus_tag="ACG8KN_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9189169.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2780 a         1579 c         1327 g         2712 t
ORIGIN      
        1 gccgattatc tttcttctat aattaaacta tcaaatatag aaggagaaaa acgaatgaat
       61 aaaaaagata ttgtaccagt tcaattttca agtgcggctc aaaaatcgca aattgacacc
      121 aagccaaaac tagcttgcag aatcaaatcg gaagataagg aaatatttat ttataatgga
      181 atggataaat atcttttgta taccttgtta aaagagttga atgccaatgc tcattgatgc
      241 aactgcattg aaacatattt ttatcgtttg tggaaaaaca gatcttcgaa aaggaattga
      301 tggactagca gctttcgtta tagaagaata tgaccttgat gtttacgatc aagcgctttt
      361 cttattttgc ggtacgaaga aagatcgctt caaggctctt tactaggatc aaaatggatt
      421 tgtattaatg tacaaaagat ttgaaagtgg gtaccttcaa tggccgcgtt ccagaaaaga
      481 agtcttactg actggccttt caatcgaaga aaaatgtacg ctaaaagaaa cgaaaaaagg
      541 tgcgatatat tagacgaaaa ccttgttaaa tcaaggattt tgtctgtttt tcgttgtcat
      601 aaaatggtat aatagatgta agaaaatcgc tagaatggtg gtgtaaaaat gacaaaaatg
      661 gaatctgaac ttcttgtaca aatcaagcaa ttaacacagt tgcttgaaat ccagcaacag
      721 gaaaatactc ttcttagaga acaaattgct gaaatgaatc gacgcttgtt tggacgaaaa
      781 aaagaaacac cacccgtaga tgggcaaatc gatttattag acgactcaac ttttaatgaa
      841 ccagagcaca ctggacaaga aagccaggaa cccattacgg tatcttcttt taaacgcaga
      901 aaacgcaagg gactcaaagc actttcatta gaaggtctgc ctgaagttcc tattcattat
      961 gaattaaaag gtgccgattg tttgtgtgaa gactgtggtc atactttaca tgatatcggt
     1021 gcgacaaggc ttagagaaga gccattgtat gttcctgccc atatagaaaa acaagtcatc
     1081 taccaacatg cctatcattg cagagaatgt gaagcattag gagaaacaaa aatcaaaaaa
     1141 gcgcctgttc ctaaaccgtt gatcaataat agtctaggtt cctcttcaat tgtgacacag
     1201 agtatttggg aaaaatttgt taaaaaggtg ccggcatatc gacaagaaaa agaatggcat
     1261 gcacttggtt tccctcttga tcgtaaaaaa atcactaatt ggcatatcaa ggtaagtgaa
     1321 atggttttag agccaatcta caattgcctt catcaagaat taagcaatca ggaggtctta
     1381 cacgcggatg agacaagcta tcgggtattg aatactcata aagaaaagac cttttactgg
     1441 ttatatgctt caggtaaaag cgaagaaaaa caagttgtgc tctacgagca cgcaaattcg
     1501 cgttccgttg atgagccaaa aagtttttta gaatcattta gcggtttctt gcatactgat
     1561 ggttacgcgg cctatggaaa gctgccggta aacaatgttt attgttgggc gcatgttcgc
     1621 agaaagtttt ttgaagcatt gggtaaacaa ccgtccccaa aaagtcatgc caaaatagga
     1681 ttagacttct gtgacaagat gtttcgaatc gaacggacct ttaataagct tagcgcagaa
     1741 gaaaggtttg tagcgaggaa ggaaacgttg cagcctgtga tgactgaatt ttttcattgg
     1801 tgtgagagtt ttgcttattt gccaaaatcg cagcttggaa aggcggttca atacgcagtc
     1861 aatcataaag acggacttca gattgtgttg cttgatggac gcttggaact ttcaaataac
     1921 cgagcagagc gtgccatcaa agagttagtg atcggtcgaa aaaactggct attttctaaa
     1981 agcttaaaag gcgctagatc aaatggaatt atattgagta ttatccagac agctgttgcg
     2041 aatgggttaa acatccgcaa atatttaaat catcttttta cagagattcc caatctgtct
     2101 tcgatgacac ctgaagcgct acgcgcttat ttgccatgga atcagcagat acaagaaatc
     2161 tgtaaataat ataagccaca aataacctga tttgagtgta tcaaatcggg ctatttgtgt
     2221 ttaggtacca ttctatgaca cgcttacgct caaaactact gaattattaa gttatgtcta
     2281 atcaaataaa aaaaccattc tcatatttgc tgaataatta gcatttagag aatggttttt
     2341 tatctttaaa atctactact caaaatctgc atgtcgagca aacatcgttt ctgagctttc
     2401 acctgtttta tccattaaag ataacactaa actatcaaag gtgagaaatg ctaattgttc
     2461 aaaacttgaa cccataggtt gtgaaaaagt atcttcttct cgttgatttt catctttggt
     2521 aatacctggt aatacaagaa cagaatctgc taattgacca atcgacgagt cttttttcat
     2581 ggtaactaaa gcaatgctta caccactttg atttgctttg gccgctaaat tttttaaact
     2641 acctgtttca cctgagccag aacagataat taataaatct ccaggttgcg aatgtggtga
     2701 actaatttca ccgacaaggc tgactgaaaa acctaaatgc attaagcgat tcgtaaatgc
     2761 ttgcatggca attccagagc gtcctgcacc agctagaaag atgtgtttag cttgaacaat
     2821 tttctcacta aattgttcta actctacaga attaatacgt tttgagttat tttgtaattc
     2881 taagataatc gcttctaaat agtttttatt ttggtcttta ttggtcatga tcttttactt
     2941 cacttcctct aatgattgat ttagtttatc aatttgttct tgcggggtat ttttcccatt
     3001 gaagatgaaa ccgcctgata caaaaatatc cgcccctgct ttagaacaaa ctttaatcgt
     3061 ttgattatca atcttgccat ctacttcaat atcatacgta taatcatttt gtttgcgtat
     3121 tacatctaac tgttcgattt taactactgt ctcttgaata aaatgcttat tctctgttcc
     3181 tggattgatt gtcattacta atacttgatc gactaaagat aatactggtt caataaaact
     3241 gactggtgtt gcaggattaa aagtaatccc tacttttgct cctgcttcct tgactaactg
     3301 aatgaccttc attgcgtgtt tggtagcttc aatgtggaag gtaacaatat ctgctcctgc
     3361 ttctacaaat cttgtgacaa ttttttctgg tgtatcaatc atcaaatgaa catcaaacac
     3421 aacgtctgtt agtggacgaa tatctttaat aacacttggt ccaaaagcca tatctggtac
     3481 aaaacagcca tccattacat caatatgcaa ataatcaaca ttcttttctt ttaagatttc
     3541 aaataactct ttaacttctg cgataggtgc atttaacaat gatggagcaa ttgtcattct
     3601 ctattccccc gtttattctc ccataatcat gagattgatt gttctagttc gctctcttag
     3661 ttgatcccaa tcgacaaaat agacacggcc catttctcct aataaaagtt taccttcttg
     3721 aacaattaaa gtcattgagt tggttccata aatagatgaa cgtaaatgtg catcggtatt
     3781 tagcatgacc catttttctg cagggtaatt gggatcagat aatcttgttc caaaatcaat
     3841 gtgttttggc cccggactgt tgtattgatt ttcactcgtc atttttggaa taatcgtttc
     3901 cattacattg ttgatgtcta catgtaaata atcatcacca aaaaagtttg tgtcatgcat
     3961 cgtttcttca aaaaagagtg agcaagttgt atgcgggctc gaaattacta acattccatt
     4021 ctcaatacct gctgtatcaa ctgtatctaa aagttctttt cgaatatcat gatacgaagg
     4081 acgccctgaa actgtttcaa tagtcaaagt gtcatgatga aaacgcatat taaacaccta
     4141 atctttcttt tacatctgca taaatggcat ctgtaccaat acctgttaat aagcccaagc
     4201 cattcgttac tttagcttta attacatcag ggacaattgt tgtacttact acagcatcat
     4261 attcatcaat tttatttagc tcatcagcaa ttttagattg ataaattttc acttgatctg
     4321 ttaatccttc ttcttccaac cattttgata attttgctac tgcaattgta gaagtagcat
     4381 gacctgctcc acaaataact aataattttt tcatattgat ctcttctttc ttattttaat
     4441 tttatgatta agctttcttc actttgttca tgtagataga agcacctact aaaacaactc
     4501 ctaatccacc tattccaatt acaccaaagt aagatgctaa taaaacaaac aaccctactg
     4561 gccataatcc acataacaac atagttacta atcctgctgt ccccacatcg aaacttgcta
     4621 atgtaaacac atcagtgata attggtgcca accatgttgc catatataat gttgtaacaa
     4681 catacaacga acatcctact aatgatctca caatatttcc tttaaaataa ggaatcatta
     4741 aacagatggc gaaagcaaag aatgctaaat ctccaaaagg taatacttta tttcctggaa
     4801 gaattagtgc tagcaataat gagattggta caagcaatag agaagttgac ataatagttg
     4861 gatgacccac agttaacgct gcatccattc caatgtttac ttggcgacca tttaactttt
     4921 tattcgtaaa ttctttcgcc gcttctgcaa ttggcattaa accttccata aacattgcta
     4981 ccattttagg cataatcttc atcactgccg cagtcataat tcctaagttt ccaattcctg
     5041 ctacgtcata gcctgccata atacccacta caataccgat aattaggcca ataacaactg
     5101 tgtcacctaa aacaccgaaa cgttttgtta atgtttctgc atctgcatct attttattaa
     5161 atccaggaat acgatcaaaa acccaattta ctggtttggc aaatagtact cctgataatc
     5221 ccatcatatg tgtgatagag attcctggat agccgaaaaa ttcgctaaca gttttttgtg
     5281 tacgatctgc taagaaccat tgtaaaatta ctgcgacaat cgcgccaatt aagccaaacc
     5341 aaaagtttcc cgtaacagat tgagtaatca tacctaggaa aattgcgaac caaaagttcc
     5401 aaatatcaac attcaatgtt tttgtccaac ccaaaaccac aaatatgaag ttaagaatta
     5461 atgaaattgg aatcattaaa attcccaatg atgtagagta agccaaaggt ccaccaacac
     5521 ctacgccaat atctaggata ttcaggccac caccgtattg ttcccccatt tgttgaattg
     5581 ctgttcctaa accatctgat aataaactta aaactaatcc taaaccaata gaaccgatac
     5641 caacagtaac gccggaaaca aatgctttac caggttttaa tccaaataat atcccgatta
     5701 caaaaataat cagtggtaaa aagacgtttg accctaatcc aataaaccat tcaaaggcat
     5761 ttaatacaat atccatctct ctattcctcc taataaactc ctgcagaatg tactaactca
     5821 ataaatgctt ctttcgtggt acatgcaagt aattcattta ctttttcttc attttgaaat
     5881 aggctaacta agttttgtag tgtatcaatt tgttcatgag gttgtgacat tgccaacata
     5941 aagactaaac gtacaggtat ttcatgagat ttatctgtca tatctgaaaa taccagtggt
     6001 tcctttaatg aggcaaaaca gatttgagag ctatttacat gtgcggaatc tgtatgtggg
     6061 atagcaaacc caatttgatt tacttctaat ccagttggaa attcttgttc acggttggtg
     6121 ataccttcta agaaactatc tttcacgcaa ccagcgtttt ttaattgttc agacattact
     6181 tgaaacaatt ctttttgatc tttcacttct aattgaaata aacataattc tttatctaga
     6241 tacattttta ttctcctttt tgttgaaaaa ttcttagtaa tttttcgcta tcatgaactt
     6301 gtaacaagcg atcgaccatt tctttttgcc caataaattg gtaaagttct tcatatactc
     6361 ctctaatctc agatacatct tcttcagcta aactaaccat aatgactaat ttgacttttc
     6421 gatcgcccca attaatcgca ttttttaatg ctacaataaa aatgttggat tcaatgacca
     6481 ttgatggatt cgcatgcggt aaagcaacac cactttctaa attagtattc cccattttct
     6541 ctctagagaa gatagaagca cgaaactctt cagtaactaa tgaattggct tctagttcgg
     6601 aaatcatcaa atccaaacat tcttctttgc tttccacctg tactcctgtt tttactaaag
     6661 atgattgaac aaatttagat atggtgggaa ctttaaccgc tttaggaatc agttcatttt
     6721 gaataaatcg cgaaggttct tctattaaat atttagtata agcttccata atattcatat
     6781 aatctgtttt acttaccaat ggagataccc ttactaaggg tataccacct gcatcaaagg
     6841 aaagagagga aataatcaaa tcaacttccc caatttcagc taaatgaaac ttatctaaag
     6901 ttgatatttc tacattatct tttgctggta aaaaccgtcg aactttattg taaattaatt
     6961 gcgcagaaga aataccgtat tggcaaataa tgatgatatt atttgctttg gactgatctt
     7021 caatagcaat ctgaaaatga atcaaaatta aggaaacttc atcgtccgtt aaagtcacat
     7081 caaattcttc ttcaaggatg gacatggcgt accatactat gctaaagagt tctgaatatt
     7141 gagatttgat attttctaat aacggatttt gaattttaat accttttttt aaacgaagga
     7201 ccattgctgg cacatgatac aataaggatt gatataatcg atcattttga gtcaaatcaa
     7261 ttttttcaat ctcactcatt ctttcaatca atttatgaac gctcatagcg tactcttcat
     7321 tgctggtttt taaattattt gtcacacgat gtgcaaatag ttgacgacat agatattctt
     7381 tatctgcgtc ataaaatgga agattcaatt ttgccttcaa ttcttcaatt aaaccatttg
     7441 ccacaatgta cgtttccata tagcgaatgc tattgaagag gaattcttct tcaacagtta
     7501 aatgatgacc tttcagtaaa cggttacatt ggataactaa agtagaaatc aatgaccgaa
     7561 catagtaatc agaaaccttt tcagtcagtt cactgtaatc attgaatagc aaatcatgaa
     7621 tgacactaat aacttttgaa tcaaacaaca tatttaaaac ttgaaaaaat gtttttgatt
     7681 ccatgtcatt cgcataatag aatacaagtt gcttgattgc tcgttgaatt gcttgttcat
     7741 taccagtaaa actaattcct tctgcagatg actgcaactt aacttctcca ctgccaataa
     7801 ttttattgat aattctcagg tcattgtata acgatgtttt actaaccata aaactttgag
     7861 acaaatgagt taaagaaaca ggcttatcac caaacaaaac ttctttaatg atctcgacac
     7921 gtcttttttc tggagaataa atatcttttt caagataatt ttctaccgat gaaatcaaat
     7981 gttcaacgtt tttcctatca cctatgagtt tgatacccaa agaaggtaat cgctcaattg
     8041 ttacttggta attaactaca taatcttcca acaattcaat atcttgataa atggtcttgg
     8101 tcgacactgc caatttatca ctaaaaaaag aagcgggttt aaagtcttct tgtcgactta
     8161 ataattttaa taaatttaca tgtctttcaa tcaaacttgt tcacctctta acgctagcat
     8221 ttgttacctc ttttcattat ttattctagc actggaaccc ccttccctca attacatcta
     8281 tgtcaataat acttgtggaa gtttgttgta aaggaaatcc aaataatctt aaaaaatgag
     8341 aaacaaaaaa ggagaaatcc ttaaaagagc taagatattc caatagaaaa tactcctt
//
LOCUS       DAZKCZ010000098         8097 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000098 DAZKCZ010000000
VERSION     DAZKCZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.101"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..797)
                     /locus_tag="ACG8KN_002152"
     CDS             complement(75..797)
                     /locus_tag="ACG8KN_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9189170.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQSYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKTVPIDHLLIETDGLHAAEWALGGIFSEKYYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(787..1524)
                     /locus_tag="ACG8KN_002153"
     CDS             complement(787..1524)
                     /locus_tag="ACG8KN_002153"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9189171.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYRNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1536..2528)
                     /locus_tag="ACG8KN_002154"
     CDS             complement(1536..2528)
                     /locus_tag="ACG8KN_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9189172.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQETKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDITYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDNPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2543..3286)
                     /locus_tag="ACG8KN_002155"
     CDS             complement(2543..3286)
                     /locus_tag="ACG8KN_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9189173.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3360..3464)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3527..4849)
                     /locus_tag="ACG8KN_002156"
     CDS             complement(3527..4849)
                     /locus_tag="ACG8KN_002156"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9189174.1"
                     /translation="MKTYDAIVIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYFVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANVVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRNPPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(4836..5801)
                     /locus_tag="ACG8KN_002157"
     CDS             complement(4836..5801)
                     /locus_tag="ACG8KN_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9189175.1"
                     /translation="MTKLIEFKHVQKKYDRKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFNEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(5798..7336)
                     /locus_tag="ACG8KN_002158"
     CDS             complement(5798..7336)
                     /locus_tag="ACG8KN_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFP9189176.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKVLNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(7752..8039)
                     /locus_tag="ACG8KN_002159"
     CDS             complement(7752..8039)
                     /locus_tag="ACG8KN_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189177.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT         2333 a         1733 c         1371 g         2660 t
ORIGIN      
        1 attaatcgaa cggtttactt ttatacaaag aaagccaaaa agaggtgtga cacaactagt
       61 gtcacacctc ctttttattt acctaatcta atgaaacgtt ccaagttttg ataagtattt
      121 ttcttaactt cttcaactga tagctctttg atctcagcga ttgtctttaa ctgttcttca
      181 agcagtgaga cataatattt ttctgaaaaa atccccccta aagcccactc tgctgcatgt
      241 agtccatccg tttcaataag taagtggtcg attgggactg ttttagctag ttgccagact
      301 gctgggttct ttttcaaatc aagaccaatg ctgaaataac agcctaaatc aatatagtct
      361 tgttgatagt ccaaagaaga ataccaatga atcaaatagt gattagggta agattggata
      421 taatttaaga tctctttttc gcatcctttt gtatgcagta caacaggttt ctcatttatg
      481 gctgcaaaag caagctgtcg ctcaaatact tttttttgtg tagtcatcgg tacattcgtc
      541 caaatattat ctaaccctat ttcaccgatg atccttgctt ttttcaaaaa aggctcaact
      601 tgttcaaacg tatagctatc tgctttccaa ggatggatac cgtaacttaa ggcttgagtc
      661 ttacttgctg caagctgtct gttttccttc cattcttctg gcgaatcaca attgatgatc
      721 gtcaaaatcg tgtgttcctg ctgaaggagt gctaaactct gactcccatc taaatgacaa
      781 tgagcatcac ttaacatcta attcacctgc caataactga taaatcgttt gcttcaactc
      841 tgattgctgc agtagccatt cacgtgaccg tcgtttttca ggcacttgaa tctcagcaat
      901 cacatgtgcc ggacgggcgg aaagcacaag aatcctgtct gataaataaa ttgcttcttc
      961 gatatcatgc gtcacaagta aagtagtccg ctttgctttt ttaggcagct tcaacatcca
     1021 gtcttgcatc tgccctctgg tcatagcatc taacgctcca aatggttcat caagaagcaa
     1081 gtaagaagaa ggattcatga ttgttcgtaa caaggccgtt cgctgcttca tcccgccaga
     1141 taattgttcc ggcaaaaaat gttcatatcc gtttaaccca aagatatcca gataatccct
     1201 tacttgttct tcattgattg ttagatgatt gattttttga tataacgtaa cgtttcgata
     1261 gacgctcagc caaggaagta acatatcttc ttgcggcata taactaaatg ctgtatggag
     1321 accagttact ttttgatcgt ctactttgat cgttccagaa gtcataggct ggactcctgt
     1381 taaagttttc agcaatgttg acttaccggc tccgcttggc gcgacaatcg atgtaaaggt
     1441 atctttaggg atagtgaaat ggatgtcttc taatattttc aggttttcaa tgactacatt
     1501 caagtggctg atttggattg acatatctat cactcctatt ttataaactg attagtgaat
     1561 agttcttgcg aaggtacatc ttgcttgatc aatccgtttt cctgcataaa agctgtataa
     1621 ttgcgccaaa ctttttcttc catcaatccc caattctcag gattatccgt ataatttttt
     1681 gacaagaatg cttgtgattc ttctaaaaac gtccgatcat tttcttttgc atatttttgc
     1741 aacagtttag cactgtcatt tggatcctta atagcttcct gatagccttt tttcgtggca
     1801 tccataaaag actgtaccaa ttctggatca cttttgatca gttgatcatt cgtaataatt
     1861 accggagtat agtaatctaa ccgttcatct aattctttca atgggatata gtttagttca
     1921 atacccgctt cttttgcttt gatcatatcc caaccttcaa agtaccattg gatatctgag
     1981 gattttccta agctttctgg cccttctcca ttgcttccta ccattgtcag ttttgaaaag
     2041 tccccgccgt ctttttccat cacagctttt aagacagctt cttcacttgg actttgccaa
     2101 cctgcatatg tttttccttc aaaatctttt ggtgaatgga tgttactgtc tgatagggta
     2161 gcgaatccag aagtattatg tttgatcact gtagcaattg ctttgactgg tatattctgt
     2221 ccatctgcgt gagcataagt gatatcttct tgataactta caccaaattg tgctttgtcc
     2281 gcacctacta gaccaatact tgtcgaatta tctcctggtt cgatgatttg gacatttaaa
     2341 ccagcctctt tataatagcc tttttctttt gctaggtaga tgccagtatg attagtattc
     2401 ggtacataat ccaaaacaag tgtcactttt ttcaaatcct ttgtttcttg tttgctactt
     2461 gtttcttttg cagtgccaca agcggataaa agcaacagta gcggtaataa aagccataat
     2521 tttttcattt gatccccttc tcttaaaaaa tataaagcat acgataatac ccttttttca
     2581 ataatgaaca agcgccattc agcaataaac tcaatagaat gacacaaatg atcgcagcaa
     2641 aaactttatc gatctggtag ctgtttttta cgcgtatcat gtaataccct aagcctgctt
     2701 gagcacttag ccattctcca acgactgctc cgctgacaca ataagtagca gccactttta
     2761 aaccggaaaa gaagccggat aaacccgctg gtattttata tattcgataa atcttccctg
     2821 tatctgcccc catcgttttc aaaaagatga tttgctcttt tgatactttc ctcaatgtat
     2881 ccgaaaaaga aactacgatc ggaaaatagc ttatcaagac aacaaggatc acttttggaa
     2941 gcataccgaa accaaaccat agggtaagta atggccctaa caccatgatt ggcaacattt
     3001 gtgaaatcac tagtaacgga taaaaagaca tttctaaaaa cttccaataa tccattgcca
     3061 aggcagtaat aaaagaaaga acgctagcta ttaccaaacc aagcactgct tcatttaatg
     3121 tcgtgacaga atgggaaagt aatacttgtg catctgccgc taatgcagaa aatacagcag
     3181 caggtccggg taaaataaag gcaggtattt tccaaactat gacaacaatc tgccaaataa
     3241 ttaaaatgaa tgccatactg actgctggat aaagattttt cttcattttg ttacctcctt
     3301 tcttctaata tgaataacaa aaaacgcttg aaatgggcac gacatagtaa cccgcttcaa
     3361 gcgtctgcta tgtttctccc ttcaccagta ttaactgaca ggttcaatgg gtctaagttc
     3421 acaacttact ctcatgtcgt ctacgacatc cccagaaaca ggtattcatt ttttaaagtg
     3481 tagcacaaga aggaaaaagc aacaagcgct ttagttatca tgctgattat ttcaataaat
     3541 aggagagatc acttgccaat gtcggatacc ccatgatata gctatccaac tcttttttag
     3601 tcaatttttg attgatgact agtgtcaaat catcgatcaa ttcgtctgct tgctcgctaa
     3661 tcactgcagc accaatcaaa taattttgct ggtcaaaggt caattcagcc ttagcaactg
     3721 gttcattgac acgatgatag gtgaaccaac tggtaagatc catctctacc acttgatctg
     3781 acgaagaagc gtgacttttt gtaacaccta cttcagcaag tttaggactg gcgtagacga
     3841 tcgtaggtat aatcggatat ttgataggtt cgcttgttgc atccgttatc cgcttagcta
     3901 cataatttcc ttcaaaagtg gcgactggtg tcaatttggg ggggttcctt gaaacaatat
     3961 ctccacacgc aaaaattttt ggattgctcg tttgcagata atcattgact gcaattccgt
     4021 gtttatcaaa gacgacattc gcttgctcaa gtgctaaaga ttctgtattc ggctgtctcc
     4081 cagtcgcaca aaaaatcata tctgctacta attccgtctc ttttccgact aaacgatagc
     4141 gagtaccttc tgaaatgatc ttttgggttt caacaccaaa agcaaactga atgccagatg
     4201 cttccatttg atggacggct tcctctacca aagacgcctc aaattctttt aatggccgtt
     4261 gattatgatg gacaatggtt actttgctcc ctgcagcatt ggcaatcgta gctagctcaa
     4321 aagcaatata accaccaccg ataaaaatga tttctttagg cagtacaggc aatgataaaa
     4381 agtctgcact agttttcaag tattcctttc cctccactgg taagatcgta ggtctttgcc
     4441 cggttgccaa aacaaagtaa tccgcatgga agacttcttc gttgacttca atagatgaat
     4501 catccaaaaa acgagcggtt cctgaaagat gatcgatttc agcttctgct aattgttttt
     4561 ttcggctttc tgggactgga tccgtaaatg ttcgtttgaa tttttgaagt tcttcccagt
     4621 tggcagttgg gatctcgtta aaaccttttc cactcaattg ttttacccga ttacgtgcct
     4681 ctactgcact taacaagact tttttaggat cacatccacg gttaggacaa gtcccgcccc
     4741 atagatcttc ttcgaccact agtactgttt tacctgcttc tttcaatcca taagcagcac
     4801 ttaagccgct cacacctgaa ccgatgacga ttgcatcata tgttttcatt tccttcctcc
     4861 tctcgactta aaaaagcaaa gatttcttta cttgtcagcg acccaatact tctttgcttt
     4921 tctactatca caatgcttgg atacaccgaa agaagcgaat atacttcttt cataggcgta
     4981 tcaaagcaaa cttgttgacg agcattttct ggtatttcat ttaagaaata tccattgacg
     5041 actaaatctt ctactcgcca ctctttcgtc tgtttcggtt cattaaagaa atgccgaacg
     5101 tagtcgtttt taggattcat caataactgc tctggtgtgt cacattggat aatttctcca
     5161 tccttcataa atgcaatacg atcacctaac ttgattgctt cttccatatt gtgcgtaacg
     5221 aaaacgatgg tcgtgcccaa ttcttcgtgt aaggacaaaa ccaattcttg taatgagttt
     5281 cttgaaatcg gatctaatgc gctaaatggc tcgtccatca aaatcacatc tgggcttgca
     5341 gcaatagctc ggatgatccc gatccgttgc tgttctcctc cagacaactc tctaggcata
     5401 cgatctcgat aaatatcagg agataagcca actttttgaa gaagttcatc tacacggtct
     5461 gctcttttct gcttttccca acctaacatt tcagggatga cttcaatatt ttgcttgact
     5521 gtcatggtcg ggaacaaagc gatttgctgc agcacatatc ccatattcca ccgcattttc
     5581 tgcagattat attcatttaa cgattttcct ttgaagtaga tgtctcctgc tgatggttta
     5641 gataatccat tgatcatttt caaagtagtt gtttttccac taccggaagg accaaccaaa
     5701 acaaagattt ctcctttttt gattgccaga tttaggtcat cgatgacata ctttctatca
     5761 tacttttttt gcacatgttt gaactcgatt aatttagtca ctgatcttcc tcctttacta
     5821 atccttcatt tttcaggaag attcgtgcta cttctgtcgg ctttttcttt tcgacattga
     5881 cttggtaatt catatcaatc atttgatcct ctgttatttt tccgcttaat ttattcagta
     5941 ctttaacgat ttcaggatgt tctttagcaa aatctttttt catcagcggt gcgccttgat
     6001 aagctgggaa taaccctcga tcatcttcta gagtcactag atcgtactcc ttgagctggc
     6061 tatctgtgga ataggcatcc acaatattga catctccatt attgattgct tggtaacgca
     6121 aacttggctc catgctttgt acacttgaaa aattcaagcc atatgcttct tggattcctt
     6181 tatatccatc tgaacgatca ataaattcca gtgtgaatcc cgctttgatc tgatcagaga
     6241 tagctgccaa atcggaaatc tttttaagtt gatgttcgtt cgcaaaagat ctcttgactg
     6301 ccaaggcata cgtattttgg taagccattg gttttaataa tgtcatatcg aattgcttat
     6361 tcaataattc ttctgcttcg tcatatgttt tttcttttgt caaatcagca cttttcattg
     6421 cttcaggtac cttcaccaag ctttctagga cagtacctgt gaactcagga tagatatcga
     6481 tttgaccttg atccagtgca ttgaacaaaa aactcgtctt gccaaaattc ggttttaatt
     6541 caacttctgt ttttgtgttg tcttcaatca attctttata catttggatc aggatttcag
     6601 gttccgctcc taattttcca gcaatcgtga tggtttcatt tttaaacaag gcagactgat
     6661 ataaagaaac gccgccaaat aaacacacca ctgctgctaa agtaatgatg gaatatcgag
     6721 gtttcttatg ctgcaaccag cgaatcagtc cgctgaatat aattgcaagc aaggcagaac
     6781 tgatcgcacc aatcaatgtc aaaacaggtg tatttcggtc aatacctaac aaaataaatg
     6841 tccctaatcc acctgctccg atcaatgctg ccaaagtagc cgtaccgatg attaaaacta
     6901 aggcagttcg gatacctgag ataatgacag gtaatgcgat gggaagctct actttcagca
     6961 agcgtctcat tctggacata ccaaatgcta ccgctgcttc ttcaatagac gggtcaattt
     7021 cagctagacc aatatacgtg ttttggaaaa tcggaagcaa agcgtaaata acaagtgcaa
     7081 tcaatgctgg aacagttccg atccctacaa atgggataag taaacctaat aaggcaagtg
     7141 aaggaatcgt ttggagcaca cccgtgatct gcaataagac ttctgccatt tttttatgat
     7201 tggctgccca aatcgctaaa ggaatagcaa tcagtgctgc aatcagcaaa gaaatgagag
     7261 aaatggacag atgttgaaat gcagcttcca gcaactcgcc ttttctttca cttaatgttt
     7321 gtataaaatt attcatgcga ccttcctttc tgttattctt taagtataaa catttctttc
     7381 ctttttccca actaacttgt cttgccttaa ccttacagca cgaacaaaaa aaaagaaaaa
     7441 aatacctcta aatatgctga caaaaaagaa tccaagcaac aaagtcggat caactttttg
     7501 gttctcgttt tttcactacg aataccataa aaatctatac gaaattgtca cttggaatca
     7561 gctatatctt ttttacacac acaactttta ttttaataaa aagaaacagc caataaagta
     7621 gaaatctcgt gggggagaac acctttattg gcctgttcaa cgaatattat actgtatcat
     7681 gacacttttt tgccaaaaat gtaaaaaacg gttgttttga catcataaac gtcttgtttt
     7741 cttattttta cttatatcac cactttattt tctatctctt tcactccata aagcggaatg
     7801 agttcttctt ttgactgata ataaacagct ttctcacggt tcttcatcaa aaaaagaaag
     7861 atgtctttca tagcaagtgg tgctttgccg tgttcaaata aaaagataaa ttgatttgct
     7921 ttatttatct ggaccattgt cacaggcacc agtttttgtt cttgagccaa aaaaactcct
     7981 tgatactctg tttttttggg tgattttgta agaattgtca ataagtctgc tgctcgcatt
     8041 tttccactcc ttatttgata aataaaaaga aacagccaaa aataattttt tttcatt
//
LOCUS       DAZKCZ010000099         8068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000099 DAZKCZ010000000
VERSION     DAZKCZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.102"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..884
                     /locus_tag="ACG8KN_002160"
     CDS             <1..884
                     /locus_tag="ACG8KN_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFP9189178.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="ACG8KN_002161"
     CDS             932..3061
                     /locus_tag="ACG8KN_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFP9189179.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="ACG8KN_002162"
     CDS             3036..3725
                     /locus_tag="ACG8KN_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189180.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="ACG8KN_002163"
     CDS             complement(4133..4819)
                     /locus_tag="ACG8KN_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9189181.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="ACG8KN_002164"
     CDS             complement(4955..5149)
                     /locus_tag="ACG8KN_002164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189182.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="ACG8KN_002165"
     CDS             complement(5199..5639)
                     /locus_tag="ACG8KN_002165"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP9189183.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="ACG8KN_002166"
     CDS             complement(5643..6488)
                     /locus_tag="ACG8KN_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFP9189184.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="ACG8KN_002167"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="ACG8KN_002167"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFP9189185.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1714 g         2358 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccgggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaa
//
LOCUS       DAZKCZ010000100         8017 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000100 DAZKCZ010000000
VERSION     DAZKCZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.103"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..279
                     /locus_tag="ACG8KN_002168"
     CDS             34..279
                     /locus_tag="ACG8KN_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189186.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(463..1212)
                     /locus_tag="ACG8KN_002169"
     CDS             complement(463..1212)
                     /locus_tag="ACG8KN_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9189187.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1398..2102
                     /locus_tag="ACG8KN_002170"
     CDS             1398..2102
                     /locus_tag="ACG8KN_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9189188.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2142..2699
                     /gene="hxlB"
                     /locus_tag="ACG8KN_002171"
     CDS             2142..2699
                     /gene="hxlB"
                     /locus_tag="ACG8KN_002171"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFP9189189.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2707..3846
                     /locus_tag="ACG8KN_002172"
     CDS             2707..3846
                     /locus_tag="ACG8KN_002172"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9189190.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            3858..4289
                     /locus_tag="ACG8KN_002173"
     CDS             3858..4289
                     /locus_tag="ACG8KN_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9189191.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            4303..5748
                     /locus_tag="ACG8KN_002174"
     CDS             4303..5748
                     /locus_tag="ACG8KN_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP9189192.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            6034..7659
                     /locus_tag="ACG8KN_002175"
     CDS             6034..7659
                     /locus_tag="ACG8KN_002175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9189193.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
BASE COUNT         2693 a         1165 c         1618 g         2541 t
ORIGIN      
        1 aataactgaa aaaattatta taggagagaa aaaatgtcaa aatctaaaaa aaatatcacc
       61 ataattggtt taggcttaac cgtatattta tttacagaat ttgtttatag acatgtgaat
      121 ttatttgcag aacctttatt tgtattatac gaatcaaatt cctcaatcgt cagtgaactt
      181 gctaatttaa tgatagcatt gctgtttact cccgcgttga gagtcataac tattttggtc
      241 tgtttagtgc tattgttttt tgttaataaa aagaaataac gtagtatttc aaaagacaaa
      301 gaagtgggat gatgattgct atagtatgtt atacgtggtg ctaggtaagt gagaataaac
      361 aaaaaaggaa acacgcaata aagtttattt caaaacttta ttgcgtgttt cttttttaga
      421 ttttttatat ctgttgcttt agtagttacc aatagttttc gcttaatcaa tcaaaacgat
      481 tttttgttta ttgtattctt tacgaacctt ttcaggaatt tcttctgaaa taagataggt
      541 aaggtcttca attggaccga cgatatgatt actgatggtg ccgaatttat cattagttat
      601 catcccaagt acttcagtag aaatacgtat catctcacgt ttaatgtcgg attcttgcat
      661 agttggcacg ctcgttccaa catctagatc tatgttataa atacccataa tataaaggtc
      721 ggctcgaatg ttttgtagtt gcttataagc ttgtacccca atattgatca tagattgttt
      781 ataaaaagta ccgccaatat tgataatttc caaattagga tgctgtgcta atgctatggc
      841 aatcggcggg ctattggtaa tgattgttgc acgaaaatct tgtgggattt cttttgctaa
      901 agccaagtta gttgtacttc catcaatgat gatgacagat tcttctttta aaaaagggag
      961 ggctttatga gctaatgcag ttttttgttc gctagctaca gtttctcggt agttaaaatt
     1021 ggtaattggc ggaccaacac gtaaagcgcc gctgtgtact ctttttaaga gaccttggct
     1081 atccaaatct ttcaaatctc gtcgaatcgt gtcttcggat acattgaaac gttgacttaa
     1141 atcactagcg ataacttttt gttctgcgtt taataaatct agaatttttt gatgacgttc
     1201 taatttcaac atgttgcgtg ctccttctta tttattcttg tttatcatgt catttatgcg
     1261 tttttatgtc aataatttcc gtttttaata cactaaaaag gcataaacaa gcaaaaacaa
     1321 aatattttta tgcagtttta tgttgactag tatttgttaa gcggttacaa tgagggggta
     1381 attaaaggag ggaatgtatg gagaaaaaaa ttattttgtc gccttcactt atgtgtgggg
     1441 atctagcaaa tttggaagga agcatccaag aactagaaag tattgggatg gattccttgc
     1501 atattgacgt gattgacggt tcttttagtc ccagtatgcc tttagggatc gaaacaatca
     1561 aaagaattag agaaatcact tcaatgaatt ttgatgtaca catcatggct aaggataacg
     1621 ataacgaatt ttttattgga gaaatgttga aaattggcgt agagagcatt acttttcatt
     1681 atgaaacaag cctccatatt gatcgatata tccagctcat caagaattct ggaacaaaag
     1741 tagggatagc tttgaatcca gcaactccgt taaatgtatt acaatatatt ttacccgaga
     1801 ttgataggat ttgtttgatg ctgattaatc caggttttgc aacaaacaag tcagaaaaac
     1861 aagtttctta cgcgctaaaa aaaattacag atctttgcca gttagtaaat gagaaagagt
     1921 tagcagtaga tatccaagtg gatggacggg tatcgttaga aacgatcccg ggattattag
     1981 atgcaggcgc aacaaatctg gtattaggaa gtacaagttt gtttcttaaa ggaaatacat
     2041 tagcagaaaa tcaaaaacta ctttatcaag cgatgaagca atcagttagg aaaaatcaat
     2101 agatgtagtg gtcctgaata atagaaatgg aggggaaata gttgagttat aaggcggcga
     2161 aacaaaatat tgtaagagaa attactgaaa cattagaccg tatttcttca aaagatgtgg
     2221 aacaattgat aaagaaaatt gaaaaagcgg aacgagtttt tttcgtgggg gttggcagag
     2281 tactgttagc gctagaagga atggcaaaac gcttagggca tctaggaatt cagacagtag
     2341 tcgtaggaca aatcactgaa ccggcaatta cagataaaga tttattgatt gttggttctg
     2401 gtagtgggga aagtgctttc ccactaacta ttgcaaaaaa agcaaaggaa tttgggtccc
     2461 aagttgccca tataggatca aacccagaca gcacaatgaa agaatattca gatcttttta
     2521 ttcgtatacc cgtacaaaca aaattagcgt taccagagga aatttcttct atacagccaa
     2581 tgactagcct gtttgaacaa agtgttcttt tgttaggaga tagtattgct ttagtgatga
     2641 tagaagaaaa agcaattgat atgtcaaagt tgtggcaata ccatgctaac ttagagtagg
     2701 tgaaaaatga aagcggtaca ttttggagca gggaacatcg gcagagggct tattggacaa
     2761 attctacatc agaatggatt cgatctatgt tttattgata cgaatcctga actgatccat
     2821 caattaaatc aagataaggg ttatactatc gaaattattg atgaaatgaa caggagtgat
     2881 ttcattgatc atgttgtggc actgaatagt ttatccgagc tagaagaggt gattacagca
     2941 acaaaaaatg cagatatctt aacaacatct gttggcgcaa taaatttagt aaaaattgca
     3001 ccgatcattg cgcaagcctt aagtaagcga tttgaacaaa agcatagcat caatatcgta
     3061 gctaatgaaa atgtgataaa tgcaagtgat gtgttgaaaa aagaaattta ttctttgctg
     3121 tcagaaaaag aacaagagaa ttttgatgaa tatgcatatt ttgtgaatac ggcaatcgat
     3181 cgacaatctt taggaaaagt tcttggagga aaaatgatct ctgttgtcga accttattat
     3241 gaatggataa tggatcgaca gcagttagat ccaactacat cttttgatat gaaaaatgta
     3301 aatttggttg atgatatgga gccttacatt gagcgaaagc tttatatcgt taatgctgct
     3361 catgcggcag tagcttattt aggcgcactg aaaggatata agacgattca agaagcaatc
     3421 caaaatatgg agattttcaa tctagtcaag caatttttaa tggagaatat agagtacttt
     3481 gtacaaaaat atcatttttc tagagaagaa ttaacatctt ttgtggaaaa aacattaaac
     3541 cgtcatggga atcagaaact ctccgatgaa attactcgtg taggaagttc ccctattcga
     3601 aaattaggcc ctaatgatcg tttaattgct cccatagtaa aactagattc atatcattta
     3661 ccttacgatg caggaataaa agtaattgct gcgggatatc attatcggaa tcttgctgat
     3721 ccagaagctg aacaactacg tcgaatgatt gtaaatgatg ggttgaaagc aacgattaaa
     3781 aagatttctc gtttggaagg taagctgctg aatgaagttg tcgctgctta tgagtcccag
     3841 atttagagga ggagaatatg ctaaaagaac aattgtcagg aaatacaatg tttatggatg
     3901 cgatcgattc atggcaagag gcaatccgag taagtgcgcg tcctttgata ggaaagggaa
     3961 taatagagga aagctatgta caagcgatga tacaaaatgt gttggataac ggaaattata
     4021 ttatattact gccactagta gcaatgcctc atgcgcgtcc agaatttggg agcaaagggg
     4081 tcgggatgag ctttttatgt ttagataagc cagtcatgtt cccaagtgaa gaaccagtaa
     4141 aattcttctt taccttttct tctgattcgc caaagggaca tttagactta atggccaatc
     4201 ttggagaatt gctgagtgat gaaaaaatgt atcaaaaatt attcgatgtg aagacagaaa
     4261 aagcattgtt agatttgata aagaagtaaa ggagagaaga aaatggaggt agcaaacaaa
     4321 aaaaaacgag tctccatgcg agctaaagtt caagcgattg gcggtttctt aacaaatatg
     4381 gtattgccaa atataggcgc atttattgca tggggaattg ttactgcatt attcattcca
     4441 actgggtggt tcccaaatga atctctgaat gaactcgtaa gtccgacagt cagctatcta
     4501 ttgccgttac tacttgccta tactggtggt cggatggttg gtggtacacg tggagctgtt
     4561 ttaggtacaa tcggaacaat aggtttgatt attggtgctg acataccaat gttcatggga
     4621 gcaatgatta tggggccact tggtgggtgg atgatgaaaa aaacagatga ttttttagat
     4681 gggaaagttc ctgctggttt tgaaatggtc gtcaataatt tttctatagg aattattggt
     4741 ttcatactga tgtgcctttc ttatctggta gttggaccaa taatccaatc agctaatcaa
     4801 tttgtaacga gtgcaattga agcattagtt tcaactggtt ttttaccgat actgtcctta
     4861 atcaacgaac cggcaaaagt cttattttta aataatgtta ttgaccaagg tgtttattat
     4921 ccattaggta tgcaacaaac attagaggca ggcagatcaa tttatttcat ggtagcatca
     4981 aatcctggcc ctggcttagg actattgtta gcttattctt tatttggtaa aaaggcagca
     5041 aaacagacag caccaggtgc aatcatcatc cattttttcg gcggaataca tgagatttat
     5101 tttccatatg tattaatgaa accactcact atattatcta tgattgctgg tggaatgaca
     5161 ggaatcatca cttttgaatt atttgatgcg ggcctggtag caggtccaag tccaggttct
     5221 attttttctt atttagctct aacgcctcgg ggaaattttt tgggtgtcat ggcgggagtg
     5281 gtcacagcaa cactggtttc cttttttgta acatcagtaa ttttaaaagc agataaatca
     5341 acagatgaag acagaaattt tgaactatca gttcaaaaat cgaaaacgat gaaatcagaa
     5401 ggaagaaaat tattaaaaga agaaaatgct agagaaatat gtaccgagaa aaaagagatg
     5461 aataaaattt cctttgcttg tgatgcagga atgggaagca gcgcaatggg cgcaacaata
     5521 ttcaagaaaa aattgcaaaa agcaggtatt actgatgttg agataaaaaa ttatcgaatt
     5581 gaagaggtac cagaagatag tgatttggtg atcgtacatc aagatttgga ggaacggact
     5641 agaaaagcac atcctaacat gaaaattgtg acattaaaga attatttgca agatccagta
     5701 atcaatggat tagcagaaga aatcattgga ttaaaaacag aagactaatt tatgggaatt
     5761 tttgattaac ctttattaaa attattttgc ctaatataaa aaggttgtga catggcttgt
     5821 gtcgcaacct ttttttgtcg cacaaataat taatcggata ttttatgttc atattttttg
     5881 tgatcaacga gaactgatgg ggcaaagata cattttagat acttttaact gttaatttac
     5941 aaaacaaggt gaaacgaata gaaaaatgaa aaaatcgata cggaagaata tgtttgatta
     6001 aaacatgcat gaagagaagg aggttgatga tacatgaaaa aaatatattc aaaggaaatc
     6061 gattgtttta gaggcattgc tgttttagca gtgatatttt atcatttgcc aaatagtttg
     6121 ctgccaggcg gattgcaagg agtaacgatc ttttttgtgg tttctggttt tttaatggct
     6181 aaaaatacga taagagatga aaaaaacaga caattcagtt ttttacattt ttactataag
     6241 cgtatcaaac gtatttatcc atttttactg ttttctttgg tcgtattcat tttcatacta
     6301 gggattagca acattcgatt gataggaaac attcgtagtg agctacccag tttgattttt
     6361 ggctataaca attggtggca gctgaatcaa ggagtatctt attttgactc ttactttaat
     6421 gcttctttat ttaaacatta ttggtcgctt tcagttgaac tacaattcta tttgatatgg
     6481 ccatttttgt ttattgtcat taagcgtatg agaagaaaac aagtgtttta cttaatctat
     6541 acattgattt ttgtttctat tctttttagt ttgtttttac cttcaactaa ggcatattat
     6601 catacgatag ctaaactttt tccattttta ttaggagttt gggggtattt taatcgaata
     6661 actattggcc gtttctttga acaaaacact tttagtaaaa tctggctttt attactagct
     6721 agtctttgtc tgattctgtt cccgattttt ccttatacac taaatgaact actgatcagt
     6781 atttgttttg ctctattgtt agccagtgtc gatgatatga acattgcaaa aaagaccaaa
     6841 atcgctagta caaaactttc ttttgtagga aaaatcagtt atgaacttta tttggtgcat
     6901 ttcccagtat tgattgctct gacaaatcta ttcaaacatc accattggaa atatttggat
     6961 atcgcaggtc tattcactgt gtttttatgt acattactca tgattacttg ctttagaaaa
     7021 ggagtgcaaa agatgagatc acccaaaatt tggacagcta tggcactaat gataatcgga
     7081 gtttctgtac tggtattacg tgctcctgcc agtcgactga ccgctgacca aaatcagttg
     7141 caaagtttat taaaagaaaa ctctagtaaa gcaaatgcag gagaaaatct gttgtcacaa
     7201 gagaagaaaa tattatttat cggtgattct gttatgctgg gggcgtatca ggaactaaag
     7261 gagacattta aaaaagaagc attggtagat gcaaaagaat caagacaaat cactgcgtta
     7321 ccagaaatct taaaggagca taaagattta gtttcttacc aacaaatagt gatcgggtta
     7381 ggaaccaatg gtgtgataac agatgaagct atagaacaaa caatgtcttt gctgaaagat
     7441 aaaaaagtat actgggtaaa tataaaagca ccaactggct ggcaagatac gataaatgcc
     7501 acattagcta cattgccaaa tcagtattct aatataacga tgatcgattg gtatagtgag
     7561 agcaaaaatc atcctgaatt tttttatgat gatgaaaccc atttgaatga aagtggacgt
     7621 acagcttatg caaagtatat tgcttcagtt attcaataag gtagtgatta cgtgtgttaa
     7681 ccttactttt tcctaatagg tgaatcaatt taaataaaaa atacttcata tgataaacgg
     7741 tgtttaagag gaggagcttt ttaatattcg tggtaaaaaa gagatgggcg aggtcgttaa
     7801 ctgagagctt cttggaaaga aatcgctttg gatttatcct ctttgttata cagaacatga
     7861 gcgagacgac aatactagga tgtatgttct gatcgaagtt ttatggttac aagcagcaat
     7921 cagcttagat aaaattaagg atcgtaggaa ccaagtaaag aaggctgtta gtaaagaaac
     7981 aaacgatcaa gtatgtttta ctcttaagaa gatgcag
//
LOCUS       DAZKCZ010000101         7825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000101 DAZKCZ010000000
VERSION     DAZKCZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.104"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="ACG8KN_002176"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="ACG8KN_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFP9189194.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="ACG8KN_002177"
     CDS             complement(2203..4389)
                     /locus_tag="ACG8KN_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9189195.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="ACG8KN_002178"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="ACG8KN_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFP9189196.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="ACG8KN_002179"
     CDS             complement(4611..5051)
                     /locus_tag="ACG8KN_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFP9189197.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="ACG8KN_002180"
     CDS             complement(5125..5664)
                     /locus_tag="ACG8KN_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFP9189198.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="ACG8KN_002181"
     CDS             complement(6279..7745)
                     /locus_tag="ACG8KN_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9189199.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2126 a         1725 c         1370 g         2604 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaact tacaagacag tgtta
//
LOCUS       DAZKCZ010000102         7708 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000102 DAZKCZ010000000
VERSION     DAZKCZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.105"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..863
                     /locus_tag="ACG8KN_002182"
     CDS             <1..863
                     /locus_tag="ACG8KN_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9189200.1"
                     /translation="YDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVKLLVKRK
                     NGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGTNQYSVD
                     NSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLSTKNNKL
                     DEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQKIQ
                     LKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIE
                     LQISEGIDKQ"
     gene            939..1157
                     /locus_tag="ACG8KN_002183"
     CDS             939..1157
                     /locus_tag="ACG8KN_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189201.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            1144..3822
                     /locus_tag="ACG8KN_002184"
     CDS             1144..3822
                     /locus_tag="ACG8KN_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFP9189202.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3813..4010
                     /locus_tag="ACG8KN_002185"
     CDS             3813..4010
                     /locus_tag="ACG8KN_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189203.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            4014..4625
                     /locus_tag="ACG8KN_002186"
     CDS             4014..4625
                     /locus_tag="ACG8KN_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFP9189204.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4610..6001
                     /locus_tag="ACG8KN_002187"
     CDS             4610..6001
                     /locus_tag="ACG8KN_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9189205.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            6217..6504
                     /locus_tag="ACG8KN_002188"
     CDS             6217..6504
                     /locus_tag="ACG8KN_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189206.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNK"
     gene            6524..7018
                     /locus_tag="ACG8KN_002189"
     CDS             6524..7018
                     /locus_tag="ACG8KN_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189207.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            7086..7400
                     /locus_tag="ACG8KN_002190"
     CDS             7086..7400
                     /locus_tag="ACG8KN_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189208.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7499..>7708
                     /locus_tag="ACG8KN_002191"
     CDS             7499..>7708
                     /locus_tag="ACG8KN_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9189209.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         2974 a         1035 c         1472 g         2227 t
ORIGIN      
        1 tgtatgatga gaatcaaaaa atttttttga tatttgaaat caaaaatctc gtagaaattt
       61 cgggaatgtc aaaaaataca atcttatcag cactagatga acttcaaaaa gttaaacttc
      121 ttgtgaaaag aaaaaatggc ttcaataaaa gtaaaatgca aatgttccca aatttttatt
      181 atttgttaaa gcctgttctg tgcaaagatg atgtttataa aattaataag ataacccatc
      241 aagaaaatag acaaaaaaac ttgatagttc aaaatttgaa ctatggaaca aatcaatatt
      301 ctgttgataa ttcaaaattt gaactatgcg acacatcaat gaaaacaaga gacaaaaaca
      361 attttgctga tagttcaaaa aatgaactag aattatataa gtcttataaa gatagtaaag
      421 atattaaaga atcacataat gatgatcttc ttgaatcact ttcaactaaa aataataaat
      481 tagatgaaga attgatcgaa acatttattg aagaaaatct tcttaaacaa atatggggag
      541 aaagtattgt gaattgctta aaactggcta gcaatagtga ttatcgtcaa ttcgacaact
      601 ggtacaaaaa attcaaatat gcgattagat ctgctgaaaa agaacaaaaa attcaactga
      661 aaattatcta tgaaatttgt aataacaagt attttgtaga tcatgttaga gaacagctat
      721 caatgacttt acgtgacttg atacgaagag caaaaacaga tcatcgtatc aaacaaaaag
      781 acgattatat ctttgcttct ctaaaaaata aagcgttaga actaattgaa ttgcaaatca
      841 gcgaaggaat agataagcaa taagaataga attgagtcaa atgaaatgga ggggtatctc
      901 cacttcattt gagaactttg aatataggag gggttatttt gattaaaaat cgtttaatag
      961 atcaagtttg gtgtctagat caaaatacag atagtctcga attaatcgag actatttgct
     1021 tcaacactat agttctcgat ttaagagctg aaaatgataa ttgtgtaact catgtcataa
     1081 ttaatcaaaa aatggctcag aagctctctg agagcctcag aaaatgggct aagggaggta
     1141 acgatgaaaa taattgaaaa aaagattgcc aataaaaaaa taaattggtt ggttgttaat
     1201 tttcttgttc tgatagctat gattattttt acaagtaata ttgctcatgc gagtattttc
     1261 aattggtttg atgacacaaa aagtcaaaca gagtttttga ataattctct ttatagacct
     1321 tacctaaaaa agtacaatgg agatttcctt agtcagttcg ggatttggct cggatgggct
     1381 gtggtaaaag gcatgtttac tgtgacagat tcgattcaaa atatgattcc agatgtgctg
     1441 gatttgttca atttcattga aagtacaggg ctaaataatg tttatcaatc agccgtaaat
     1501 acaattgtca ttggattaat gatcctttca ttgatgtttg ttggttacaa aatgattact
     1561 ggaaaaggaa cagtggattt aaaatcggtt ggaatgaacg tagtgatgtc tatagcatta
     1621 atacttttaa tgccaaccat gatttctagt ggtattcaat ttgctaaaac attttataag
     1681 gactctacta cgattacaaa ttcagatgac ggagtcgctt ggtcattgat aaaacaagga
     1741 gtaacagatt tagcatatat taataaaaca gatcagtact caagcattga taaaacggag
     1801 gatcgaaata atttaacacg aaaaaatttt caacagacgg atctcacaca agtgttaacg
     1861 gataaagtga ttgataaatt ggaaaaagaa aatccaattg cggacaatct tcgttatgaa
     1921 ttagttgaaa attctaataa tgaatttgtt gctacaaagt tttctgacaa cttcttatca
     1981 acattttctg atagcttgaa atcaggctat taccgatacc aagcaaatct atgggggatt
     2041 tcaattgggt tgacagcgtt agcaatcgcc tatgtgttca gcgcgtttgt gatcattaca
     2101 gcaatactag aacttgcatt taaacgtgtg ttaggtgttc ttgtttttgc aacagatatt
     2161 gagacaggac agcgttcaaa agttgttcta tcagatattc tacaatgtta cttaactgtt
     2221 gggtttcaag gatttgggtt atcaatgttt gctatgttta tcaattttct gaactcagga
     2281 caaggtatct caacaaatat ttttataaaa acaattgctt atatctgtgc cgtttttgtt
     2341 ttaatcaaag gcagtggaac agtcatgaga tactttggtg ttgatattgg actcaaagaa
     2401 ggctatgggc aacttgcttc tgcttttgga atgggcgcaa tgctgtttag aaaaggatca
     2461 aatggattta atcgtgctaa aggaagcgga gaaggagaag atcgaaaacc agaaaagaat
     2521 ttcggcgaaa ctctatcaaa aaaagctggc aaaactggaa gagctttggg atatgcccat
     2581 gaacgtggat tatccggttt agcttcagat ggagctacaa tggctagtga aagagcaaca
     2641 aaaccattta agagtatgcg tgatatagct aatgatacca agaataagtt taaagaaggt
     2701 ttagatgatg gtacagtgtc cgctattaat aaaaatagca aacctatgtt agctaaaaat
     2761 aaagaagatg aaactggaaa atatgctgat tccgtgccaa cacgtctttc agatagaaaa
     2821 gatggtgcta aagttgaaaa tgccgataga attatgtcta gttctgaacg tatgcgtgaa
     2881 gcaatgaaaa ataatgctga atcgaataat aatcctgcaa gcgcaattca gcaaaaagtc
     2941 caacaagata ttgaagaacg taaaaatgct atgcacggtc aagctaagtc tgctgaagaa
     3001 ttaattaatc agaagcgtca tgaagcgaaa tacacgcctg aagcaatgaa tcgcgaggaa
     3061 ataatgttaa ggaaacgtgt agaagggcgg acaggcgcta atattgacgg aaaagaagcg
     3121 ttgaccaaag aaagaattca agaagctaaa aattcaaata caggattaga aaaattagtc
     3181 aaagaaaatc cacaaaattc tacaagtagt catggtggcc gatcagtaga tgtccgagaa
     3241 aatattcaag gttcactgaa tggtcatgtt ggacggaatg ttgatgtaag agagaattta
     3301 caaagttctt caaatgctca aggtactaag acaattgatg ttcgagaaaa tctgcaaagt
     3361 agcagtacaa gtcaaccaaa aacagtagat gttcgagaaa atgtacaaaa ttctacaagt
     3421 agtcaagggt ttaaatcagt ggatgtccga gagaatgtgc aaggtagcag tgcaagtcaa
     3481 ccaaaaacag tagatgtccg agaaaatgta caaagttcta caagtagtca aggattcaaa
     3541 acagtagatg ttcgggagaa cattcaaaaa gatagtggag aaatgaaaga aaaaacacaa
     3601 aaaatcaata tcgttgaaga cagaaagtca gcgaaaaaat ttgattcaaa tcatgaaaca
     3661 gtgattattg atagtgaaat ccgtgaaaat gataagggat caaaatcaag acgaagattc
     3721 acttatgaag acaatgagtt attcagagat acacttaatg atcctaatcc acttttcgat
     3781 aatttattaa gaaataaaag ggggggattg tcatgcaatt aattctaaag attagtacgc
     3841 atgatttctt agaacttgac gaattatgtg aatcagtaaa gaacgcaaca aatgaaaaaa
     3901 tcacggtcac taattatatt gaactgatga ttcaagaaga attaaagatg aaaaatgaaa
     3961 tagagagcaa tttcaaatgt tgctctctag aaaaaaatgt gaggggataa tcaatggatg
     4021 ataaacaaaa aatgtacgaa aaaaatcgca aacatagtga ggccttaaat tttcgtgtcg
     4081 ataaaaagac ttatcttgaa ctgacattga ttgcaaaaca aaggggaatt tctagagcag
     4141 acgtttgccg tgagcggtta aagggactca aatatgaacc gccgttgtta gctgaagatg
     4201 atagggtagt tttaaccaaa cagttaaatc aaattggtaa caatttgaat caagcggtaa
     4261 aggcattaaa tatcattgct aaatatttta gagaagaatc tgaagataaa aaagaaaaga
     4321 gcctacattt tagttttgat gctagccgtt acaacatttt taaagatgaa cttgcgaata
     4381 gcatagcaaa aaagttgatg attagttatc cagatgacat ttttttggag tccaatcgga
     4441 gtgccccctc tttttttaaa ttatcagaag tagaaaaaga atatttcgaa tattggatag
     4501 aaaagcaaac tagaccgggt acgaaagaag agtattccca aaaagttgat cggaatttaa
     4561 ttcactcaat tcagattatt gcaagtttaa aagatgaggt gaaacaactt tgggagctac
     4621 tataacatcg aaaggacgca agaaaaattt atcctcgcta ttacaatatg cagctaatcc
     4681 ttcaaaaaaa gaaaaatgtg ttgcagtctc aggaataaat tgtagtgatg atagtgagga
     4741 agcactgcaa gaaatgaatt tagtacgtac caagatgaat aaatctaaag atatagcaca
     4801 agcgtttcat gttattcatt cgtttgataa gagtacttct aaagagttaa gtattgataa
     4861 aatgcacgaa atagctgtcg aatttgcaga gaaagctttt ccaaattcgc aaatcattgt
     4921 tgcaagtcac aatgataaag atcattttca tagtcatctt gtcattaata atattaacat
     4981 ggaaacatgt aaaaggttgc gtattgaccc taaagattta gaactaatgc gaaaaattaa
     5041 cgatcaagtc ctcaatgaac atggtcttga atcagtgaat aatgagtact atgaaaactt
     5101 agaaaagaga accaatcttt attcaaacga tccttctaag atgaaatatg gaaaaattaa
     5161 ttatcaagag tttgtgcaaa atgcgatcga agcaacgttg gaaaaatgcg aagtgaattc
     5221 ttttgaagat ttttcggatc atttggcaaa agaatataac attgaagttt atcaattttc
     5281 aaaaacaaat aacaaacttg gctatgttct ttacaaacaa ggtgtaaatt tctatgagcg
     5341 tagaggtgag ttaattactt taggaagaga taaacaatct agaaaagaaa aaagtcagta
     5401 tgtcgaacgt acttttagtg caagaaagtt agggaaaaga tattcatttg aatctgtttc
     5461 tgaacaactt gaaaagaatt tagaagaatc acatataaaa aattttttta atatggaaag
     5521 agagcaagtt atgtcgctaa ttagtggagt aaattacaaa actggtgagt taaatgtacc
     5581 gtttgtggaa ttagattcca tcgcacaaaa gcaatttagc gaccgtgaag gcgatgaaaa
     5641 attcattaaa tatgatgact tcttgcgaga ggtagacaag cattttgtaa atacaatgtc
     5701 tttagaagat gatcaagaat acacgattaa acttcatacg gatgtgaatc ctcaaacttt
     5761 acgtagtgcg aaaagtaaaa caatttctat taaaaagact gatcagaaag agttatttct
     5821 accggcaaag ggttttgaaa attgttctca tacaccttct gctaaacaga ttcactatca
     5881 aatcagtcgc ataatgaatg acgatgtaaa aatgtatcgt gctaaaaagg attatgaatt
     5941 cacacaacaa atgattgaaa atcagcaaaa aaggcaacaa aattttgaaa tggaattctg
     6001 aatgggcttg ttagtggact ttccagtttt ttgcctatga agagttgctt ggaaagaagc
     6061 agtttttagg acatacgcca gctacccatc tacttgccag tcaatccgcc ccgctcacgc
     6121 taggctactt tctggcaagt cagatggtgg gtaaatctct gagggagcaa gctccctaca
     6181 gactccctcc caaaaaaaga aaggtggaag aaaaaaatga actatccata ttttaaagtc
     6241 tcggcttcag aagaaactaa agaaatattc aacaattttt acaaccaaaa caaaggagtt
     6301 tttggttcaa aggcaaatat gtttcgtgtt atggtttcga atttaccagt gttagctagt
     6361 ccatcaaata ataaatttaa tgatcctgaa agcattaaat ttgaacaaaa aatttcagaa
     6421 ctagaatcaa tgataagtaa tgaagtaatt gaaaagctgg atgacattga tcaaaagtta
     6481 tcgtattctc taaaaaacaa ataaaaaacg gaggagaaaa aagatgttta aagtttactt
     6541 cccgtttcag tggctcgtta aaggaattct tttagtgcta ctaggattga ttctttttgg
     6601 aagttatcta acaattaaag ccagacattt tccagaagaa aagccaataa aaattgtaac
     6661 aaaagattta aaaataggtg aaacttatca ggcagatggg gttgaactaa catttaaaaa
     6721 tattgaagtg accgaaaaaa gaaatgaatt tgcacaacat gatcgtgtat tacaagtgac
     6781 ctatacactt ataaatgata cagaaaatcc gatacgtcca caacaatggt cgattgaaaa
     6841 taaggatggt gaagaacttt atgagtatga tatgatattg gataatccag agtatattga
     6901 aagtggcaaa gaagaaacag taaattacta ttatgaatta ccgattgaaa ggcaggacta
     6961 ccaaatcgta gtacgtagag agaatggtta catagttggg ttgccattta ctttataagt
     7021 tttattttgt tagtagtttt taaggtaata tcagttttgt gatttttgaa aatggaactg
     7081 ggagaatggc tgaggaaaat aaagttgttc cgtttgaaac attagtgata aataaggaag
     7141 agtttgagca actttttctt gattttttta ttcaatatga catgatttat gattttagtt
     7201 cgtcactaga tttatttgaa ttagaagcag aaataataga aaacagtcga gagaagaatg
     7261 gtaaaatcat tatttcagta gaaaaattca atgaattatt ctcacaaaca tttgaaatag
     7321 atccagacta ttatcaacca caagaagttt ttcgagagtt tagaaataag attattggtt
     7381 ttagtcaaac tacaaaatga aagcttaata aagcaagtgt tgatagaatt attcctcaac
     7441 gcttgcttta ttttatgggc acttcataat agaggtatag agaagagagg tattcatttt
     7501 ggatactatt actccaacaa acttcaaaga aaatataggt aaagtgttgc aagaagtagt
     7561 tcattcaaat tctgaaatag aaattactag ggattctaaa aatggattga acgatggcgt
     7621 cattatgatt tctaagagaa aatgggagtt gttacaagaa gaactttatt tacaacgaac
     7681 gggtacatgg gcttatgtat ctgattta
//
LOCUS       DAZKCZ010000103         7667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000103 DAZKCZ010000000
VERSION     DAZKCZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.106"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..691
                     /locus_tag="ACG8KN_002192"
     CDS             <1..691
                     /locus_tag="ACG8KN_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9189210.1"
                     /translation="TILYNVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYF
                     LSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYN
                     SIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADF
                     GLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTI
                     ARRFDSDSALF"
     gene            703..1623
                     /locus_tag="ACG8KN_002193"
     CDS             703..1623
                     /locus_tag="ACG8KN_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9189211.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1664..3121
                     /locus_tag="ACG8KN_002194"
     CDS             1664..3121
                     /locus_tag="ACG8KN_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9189212.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3466..4080
                     /locus_tag="ACG8KN_002195"
     CDS             3466..4080
                     /locus_tag="ACG8KN_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9189213.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4073..5797
                     /locus_tag="ACG8KN_002196"
     CDS             4073..5797
                     /locus_tag="ACG8KN_002196"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9189214.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5794..7245
                     /locus_tag="ACG8KN_002197"
     CDS             5794..7245
                     /locus_tag="ACG8KN_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9189215.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2617 a         1289 c         1516 g         2245 t
ORIGIN      
        1 tacaatttta tataatgtag catttatcct tttgaatctg ctgatctcag tcgtatttgc
       61 aatcattatg agcgagttga gaaataaacg actcgttaaa gtttatcaaa cgatgtcctt
      121 actaccttat tttctatctt gggtcattat cagttacttt gtttacgctt ttttaagtcc
      181 tgacaaagga atattcaatc agtggatcac tggacatgga ggagaagctg tcaattggta
      241 taatgaaccg aaatattggc cgtttatctt ggtgttcatt ggtacatgga aagggattgg
      301 ttataacagc attatttact ttgcatctgt tatgggaatc gatccgactt attatgaagc
      361 tgctatggtt gatggagcta gcaaatggca gcagatcaaa catgtgacga taccacaact
      421 tattccacta atgacgattt taacgatttt agccgtagga aacattttcc gagctgattt
      481 tggcctgttt tataacattc ctaggaattc cggtgcatta tatgatgtga ctcaagtttt
      541 ggatacgtat atctacaatg gcctgacatc cacaggagat tttggtatga cggctgctgc
      601 aggactttac caatcagttg tcggctttgt cttgttgatg atcacaaata cgatcgcccg
      661 acgttttgat agcgattctg cattattcta aggaggaaaa aaatgaaaaa aaagaaagtg
      721 gagagagtcc aagtccgctc tttcaataag acggctaatt ttttctctaa tataatgatt
      781 gcactctttg ctatttcctg tattttgcca tttatatttg ttatcgccat ctcttttacc
      841 gatgagtcca tattgtcgac tcacgggtac agtttttggc cagaaaagtt cagtatattt
      901 ggttatacgt acttgttcga ccagatgcag gataaaattt tccaagcgct tttcgtcact
      961 gtacttgtta ctgttttagg tacactgatc aatagcacgg ctacttcttt atatgcttat
     1021 gcgatttcac ggtcaaactt tccattccgt cgttttttta ccgttttttg tttgattacg
     1081 atgttgttct caccagggat ggtggccaat tatcttgtta tgacaaatct tcttcagtta
     1141 aaagatacga tttgggcatt gatcttgcca atggctgtca gtcccttcaa tattattgtg
     1201 atgcgcacat tctttaaacg ttcggtctct gattcgatta ttgaatcggc acggatcgat
     1261 ggagctagcg aactgcgaat atttgttcag atcgttttac cgttagctgt tccaggtatt
     1321 gcgacaatca gtttatttgc cgcattgggt tactggaatg actggttcaa tgctttacta
     1381 tacatccaag atgataagct agttccacta cagtatcttt tgatgaaaat ccaaagtaat
     1441 attcaatatc tgacacaaaa ttcaggagca ggaagccaat tgacaggcgg attagcctca
     1501 gttccaggag aatctgccag aatggcgatc gtagtcatct cgacgttgcc tattgcgatc
     1561 agctatccat ttttccagaa gcattttgtc aaaggtctaa caatcggcgg agtaaaagaa
     1621 taaagaaaca aaaatataaa tcataaaatt aggaggaaga aaaatgaagt tatggaagaa
     1681 actagccttt acaggtgttt cagcgttgtt gttgggaaca ttagcagcat gcggaggttc
     1741 tggttctaaa gaccaggcag aagctagcaa ttcggacact ttgcagatgt atcaaatcgg
     1801 agataaacca gataatttcg atcaattgat ggaagtagca aacaaacgaa tcgaaaaaga
     1861 gatcggtgta aaagtcaata tcaattacat tggctggggc gattacgaaa agaaaatgaa
     1921 cgtcatcatt tcttccgggg aaaattatga tatcgccttt gcgaacaact atgtttccaa
     1981 tgcccaaaaa ggagcctttg ctgatttgac tgaattagcg cctaagtacg ctgaaaaagc
     2041 ttataatgat ttagatgaag catatattaa aggaaatctg gtcaatggaa aattatatgc
     2101 tttcccagtc aatggaaatg tctttgctca acaagtactt acttttaaca aagctttact
     2161 agataaatac gatctttcaa ttgatggaat ccaatcttac gctgatgcag aaaaagtctt
     2221 gaaggaattc catgaaaaag aaccaaacac ggcagctttt gccattggac aaggcttcaa
     2281 agtccaaggg aactttgatt atcctttagg gaatacgtta ccatttgcta ttgatctgga
     2341 tggagatgat tcgaaaatca tcaaccaata cgataatgaa acgtatatcg gactgttacg
     2401 tacaatgcat agctggtaca aacaaggact cattccaagt gacgctgcaa cgagcaataa
     2461 ggattatcca ctagaaggaa atacttggct aatgcgtgga gaaacacaag gaccgtatga
     2521 ttatggcgat acaattttga caaatgcagc aaaacaagaa cttgtctcaa aagcaattac
     2581 tgttccacta aaatctacag ctcaagcaca aatggcaaat tttgtcgtat caaatacttc
     2641 aaaaaataaa gaaaaatcag tagaacttct agggctgctt aacagtgatc cagaattatt
     2701 gaatgggctt gtttggggga tcgagggaga agcatgggaa aaagtcgatg gttcagatga
     2761 taaaatcaaa ttgctagatg gttatcagcc aaatacacat atgtctgctt ggaatacagg
     2821 aaacaacaag attctatata cacaagagtc tattacggat gacatgattg ccaaacgtga
     2881 tcaatcgatt gctgatgcag aaacttcacc gattttagga ttcagcttca acactgacag
     2941 tgtcaaaaca gaattaagta atatttctaa tgtcatgaat caatatttag atggattgaa
     3001 tacagggact gttgatccag atgaaacatt accgaaattg aaagacgctt tggacaaggc
     3061 tggctatgat aaggtattga aagaaatgca aaaacaatat gatgagttcc gacaagaata
     3121 ataatgaata aagacaaagg ggttgtgaca aaagacgtgt cacaacctct tgttccgaat
     3181 aaacggtgtt caaaagccag tttctcggta ttaagctaaa tatatgtaaa aatgtggaaa
     3241 gccatattca caaaattttt cttactactc gaaactaagc ggctatttca caaactcttg
     3301 gtaaacggtg ttcaatcagc taaccctcgg tattcgctca caaaacgaca aaatatgagg
     3361 aactattttt ccgtttgttt ctctaataat caggtcaagg tgctgattgc ccaacctttt
     3421 tttgtataat gagaaagtat aaacttaatg tttggaggag aagatatgtt agggaaggtt
     3481 ttggaagagt tatttatccg gatctgggtc attataaaac taaccttgta tttctggata
     3541 tatacatttg ccggcgggat catctttgga ttaggtgcgg catggaaaac agtcaatgaa
     3601 ctattttatt tgtacggatt cgagtataaa gaaatcacaa taaaaagagg ctggaatata
     3661 tataaaagaa attttttaag aggcaatctg ctatttagtt tatttttatc tggtactgct
     3721 ttattgagtt acaatttata tttatctgtt caaatcaaag ggctcatttt tttagctatt
     3781 gattttattt tactttttgc cttgctctgc ctatttgcta cttatcaata cagtatgatt
     3841 ttagatagtg aatatacgat ctctgttcct aatctgctaa agctaagttt tatttcggtt
     3901 ttttccagct tttcgtcttt tctaaaaata attattggta gcggtataat tttaggcgtg
     3961 acttggcaat tcaaaggatt gatcctgttt ggtgtcattg gtcttttgac ggtttggaat
     4021 ggtacgatga caacacattg gcgtgaagaa ttagataaac agttggaaag ttatgaataa
     4081 gtttcggatt ctattttcaa aaggaacctt actaaatcgt ctgttgcgga aatacgcggt
     4141 tatcatgatc ggtgtaacga tgactgctac cgttattttt agtatccata cttgggaaca
     4201 aaatcaaaaa caggcagaaa acatgacaag tgatgccgtt caaagcacaa gcagaatgct
     4261 gaatgataaa acaaccttga gtaggatcat caaaaaccag cttgtaggga attccgaaaa
     4321 gatcgaaaat gtgacgacct acttaacaaa acccatagat cagtatctga tgtatgtata
     4381 cgaacagcaa aacagtaccg atgaattagt ttctttccca aatcaaatca aagatctcta
     4441 cgcaaattat gaagaactaa gtgcgatata tatcgtgttg aatcaactac cggagtatta
     4501 tgaatcttct agagaaaaca aaggtgggga aataaaaaag gggacaccaa aattactaga
     4561 cgccttttat atcaagttgc ctatcacaca aggaggagct gagtcaggaa gtatttttat
     4621 tggttttcaa aagaaagaac tagatatgat tttagaaaat ctaacgtcat tcaatggact
     4681 ctctttatac atgatttctg gaacgacaaa tcgactatac acattccatg ataataaaat
     4741 agctaaagaa acgtttgaaa gacaagaaat gatcatcaca gagagtctga agaaaaattc
     4801 acaattgccg attgacgatc tggaaaaaaa caattttgtt caacaccaag aactgtctga
     4861 tgattatcgt atattagctg tactagataa aaaaagtgta aaaatcgaaa cgggaaaaaa
     4921 tcttgccccg ctgatcattg gtatcattat tttagacaat atactgctta tctttcttta
     4981 taaaacattc aaacgatatt cacagcaagt atctatcgtc atgcaggcca tggatcaaac
     5041 agcagcaggg aatttagaga cgcggatcga cataacaaac acggaatatg aactgaagga
     5101 actttcaatc ggtatcaacg aaatgttgga tagcatcaat cagtttgtag aagacatcta
     5161 caaattagaa atcaaacagc aagacgcaca tatgcgggcg cttcaagcac agatcaatcc
     5221 acacttttta tataatacat tagaatatat ccgaatgtat gcaatcagcg aaggaagtga
     5281 agaattagca gatgttgtgt atgcgttttc cgctttgctt cgaaacaata cgaatcaaga
     5341 gaaaacgatc actttaaaag aagaactgga cttttgcgaa aaatatgtct atttgtatca
     5401 aatgcgttat cctaatcgag tagcctatca ttttatgatc gatcctgatt tggaaaagat
     5461 cgaagtacct aaatttgtga tccagccatt agttgaaaat tattttaaac acggtattga
     5521 ttttactcga ttcgataatg cgctaagtgt caaagtcttg caggaaggca aacgggtaag
     5581 gatcatcatc aaagataatg gaaaagggat gactgaaaaa cggctaaaac agattgaaga
     5641 aaaactgtct catcctaaag tggaacttca cggttctatc ggtctgcaaa acgtgaatga
     5701 acgtttacgt gccagcttcg gcagcagtta ttatatgtcg cttgagaata atgagacagg
     5761 tggactgacc gtttctatta cattcaagga gggatgagcg tgtatcaagt gttattggta
     5821 gatgatgaat acatgatttt gaatgggtta aaaaagataa ttgattggca aagtttaggc
     5881 tttcaaatag tagcaacagc agagaatgcc aaagaaggac tagctgtact tgaacagcga
     5941 cagattgatt tagtagtaac ggatgtgaca atgccagaaa taaatggact ggaatttatt
     6001 gaagcggctc aaaaagaacg tcataatttt gaatttatga ttttatcagg ataccaagaa
     6061 ttcgattatt tgaaaggagg tatgcaatta ggcgccgtga attatttgat gaaacccgtg
     6121 aataagttcg aattggttga aagcctcaag aaaatcaaaa cacggttaga tcagcaaaat
     6181 gaacaaaaaa atcaacaaga aatctatcaa gaactgttat tcagtcaatg gttgaatgaa
     6241 gaattggatg aaataagtga agaagaaata ataggacaaa taagtgagaa gcagcatagg
     6301 atagtattga ttcaactggc aagaactcat gatactctta tcaatagatg gttgcaagaa
     6361 cggcaacaaa tcttttacta tcaacgaaat tacggagacc gtgtattgat cacgttgttg
     6421 ttggaggcag atgaagttga tggcttctgt tgttttgttc aagaaaactt tcctgatcag
     6481 gaatggctga tcagtatcgg ggaagagaca gattctttgg acaaaatccc tgaaacattc
     6541 caacaagcga aagacaatct acagttgcat caattttatg gagataaaga cgagcatgtt
     6601 ttttacgcag tacaagcaga aacaaaagat caaaccatcg atttttcaag tttcagacgc
     6661 gtgctgcaaa ataaacaatt ggaagtcgct caaaaaatga tcactgattt ttttgaacag
     6721 tttcagttag cagccatgat gccggaagat atacgatatt ccagtttttt actgtttatg
     6781 gagattcaac gggaattgat tgatctggaa gatgaggaat atttacaagg aatcgaaaaa
     6841 atccatcagg caaaaacggt gcaagaactt cagcagctac ttctgtcatt catcaaaaag
     6901 catcagagac agaaaaagta cagtgacaat gtggaaaaag taatcgtaat cctgcaccag
     6961 cattatcaag aaccacttac actcaaagaa gtgtcagaaa acctgcattt aaatgtcatg
     7021 tatctaggac agctgttcaa aaaagaaacg aaaaaaagtt tttcagctta tttgaaccat
     7081 ttacgtatgg aaaaagcgaa acaactgctt ttgcacagta atcaaaacat caatgagata
     7141 gcaagtgaga ttggctacaa caacaccacg tatttctcta aattattcaa gaaaatcgtc
     7201 ggcagatccc ctaaagagta ccgagaaaac agtggagaat cataaaagaa aaagccaatg
     7261 taatgaaata acttataaaa aattgtctag aaatctaatt ttgggctgtt cttattactt
     7321 gaggcaaggc agctttttca tgacctctcg ataaacggag ttcaaaagcc agtttctcgg
     7381 tattaagcca aatttatgca aaaatgtgga aagccatttt cacaaaattt ttcttaatac
     7441 tcgaaactaa acagcttttt cacgacctct ccactaaacg gtgttcaatc agctgaccct
     7501 cggtattagc tcaaaaaatg gcaaaatatg aggaactatt ttgccatttg tctctctaat
     7561 actcaggtca gaacgctgat ttcacaacct ctcaataaac ggtgttcaaa agcttgcatc
     7621 tgcggtaata agaccaacca aacgaaaaat atgaggatct atttttg
//
LOCUS       DAZKCZ010000104         7628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000104 DAZKCZ010000000
VERSION     DAZKCZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.107"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..714)
                     /locus_tag="ACG8KN_002198"
                     /pseudo
     CDS             complement(16..714)
                     /locus_tag="ACG8KN_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            793..969
                     /locus_tag="ACG8KN_002199"
                     /pseudo
     CDS             793..969
                     /locus_tag="ACG8KN_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1145..1714)
                     /locus_tag="ACG8KN_002200"
     CDS             complement(1145..1714)
                     /locus_tag="ACG8KN_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9189216.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="ACG8KN_002201"
     CDS             complement(1788..2111)
                     /locus_tag="ACG8KN_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189217.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="ACG8KN_002202"
     CDS             complement(2265..3077)
                     /locus_tag="ACG8KN_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189218.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3404..4279
                     /locus_tag="ACG8KN_002203"
     CDS             3404..4279
                     /locus_tag="ACG8KN_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9189219.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4293..4616
                     /locus_tag="ACG8KN_002204"
     CDS             4293..4616
                     /locus_tag="ACG8KN_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189220.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4613..5365
                     /locus_tag="ACG8KN_002205"
     CDS             4613..5365
                     /locus_tag="ACG8KN_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFP9189221.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="ACG8KN_002206"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="ACG8KN_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2273 a         1512 c         1372 g         2471 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacctattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 aacttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgagaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg aatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagcttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaac ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctcgaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacctt
//
LOCUS       DAZKCZ010000105         7164 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000105 DAZKCZ010000000
VERSION     DAZKCZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.108"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..676
                     /locus_tag="ACG8KN_002207"
     CDS             <1..676
                     /locus_tag="ACG8KN_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9189222.1"
                     /translation="KKIAPDHQIAIYTHTDKDHIHNHIVINSVNLETGKKFQAHGQAF
                     LNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEKNEPVWKDEIRIAIDQTMKN
                     KKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRSNRLGKDYEKETILHELDR
                     RERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEIRPARRNHEQHVERGTDH
                     QQELSL"
     gene            636..914
                     /locus_tag="ACG8KN_002208"
     CDS             636..914
                     /locus_tag="ACG8KN_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189223.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDL
                     QVKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
     gene            916..1605
                     /locus_tag="ACG8KN_002209"
     CDS             916..1605
                     /locus_tag="ACG8KN_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189224.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYKEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1726..1812)
                     /locus_tag="ACG8KN_002210"
     CDS             complement(1726..1812)
                     /locus_tag="ACG8KN_002210"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9189225.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
     gene            2457..3194
                     /locus_tag="ACG8KN_002211"
     CDS             2457..3194
                     /locus_tag="ACG8KN_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9189226.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            3223..3744
                     /locus_tag="ACG8KN_002212"
     CDS             3223..3744
                     /locus_tag="ACG8KN_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9189227.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLDRLHEYEQENDKNVYISRKNVVVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEEPEEEVLTSVNKENN
                     NAITTEKKWWKFW"
     gene            3828..4280
                     /locus_tag="ACG8KN_002213"
     CDS             3828..4280
                     /locus_tag="ACG8KN_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9189228.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKVMLKNHTKTLILLFHFVIFLSLVFTLIQSKFFYFS
                     HSIATWLLIFFLSLLLVINTLIIIFNKHKNLFLHSVFPIILLSFLYFV"
     gene            4700..5080
                     /locus_tag="ACG8KN_002214"
     CDS             4700..5080
                     /locus_tag="ACG8KN_002214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189229.1"
                     /translation="MKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPIEE
                     QAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDAD
                     NTIKYIYTVKKIQMENIQEVFHNF"
     gene            5350..5844
                     /locus_tag="ACG8KN_002215"
     CDS             5350..5844
                     /locus_tag="ACG8KN_002215"
                     /inference="COORDINATES: protein motif:HMM:NF017364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFP9189230.1"
                     /translation="MINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTY
                     PNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGY
                     GNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNT
                     ITNW"
     gene            5817..6260
                     /locus_tag="ACG8KN_002216"
     CDS             5817..6260
                     /locus_tag="ACG8KN_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189231.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            6564..6944
                     /locus_tag="ACG8KN_002217"
     CDS             6564..6944
                     /locus_tag="ACG8KN_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9189232.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            6926..>7164
                     /locus_tag="ACG8KN_002218"
     CDS             6926..>7164
                     /locus_tag="ACG8KN_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9189233.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYA"
BASE COUNT         2720 a         1172 c         1230 g         2042 t
ORIGIN      
        1 aaaaaaaata gcccccgatc accaaatagc tatttacaca catacagaca aagaccatat
       61 tcataatcac attgtcatta actccgttaa tctggagact ggaaaaaaat ttcaagctca
      121 tggtcaagca tttctaaaca aatgttatga cctaaatgac gagatttgtc ttgcacataa
      181 cttaccaatc actgagcgag gaaagaaacc agaaaaacga accatgtctg aaataaaatt
      241 aaaagaaaaa aatgaacctg tttggaaaga cgaaattcgg attgcgatcg atcaaacaat
      301 gaaaaacaaa aaaaccagaa cctatgacca attttgtaat tccctcaaaa cattggggat
      361 tcattgcttc aatcgcggga aaaatttcac ttacgaattg atccacaaaa agaaaaaagt
      421 acgatccaac agactaggaa aggactacga aaaggagacg attttacatg agctggacag
      481 acgagaacga acctataacc aacagcgaac caacgagcta cgaagagctc gagctagcat
      541 tcgcgaaact gcagaagaag tacgaagaga acaacgaact cttacgcctg aacaaccgga
      601 aattagaccg gctcgaagaa accatgaaca acacgttgaa agaggaacag accaccaaca
      661 agaattatcg ctctaaaacg ttaaaagaca tagaaaacga aataaagaaa caacgtgctt
      721 ttctcgttcc ttatacgcaa gaaaggatac aaactacgat tgatttacaa gttaaaaaac
      781 tccatcaaga gctattagac cagcgaaata aggaacagaa aaaggtgctt attcaacgat
      841 cattggattt tcttgccccc ttatgttgct tagtcctgac actgttcgca ttttggttag
      901 tatggggagg ctaaaatggc attttattta ccctatctac tcatttttgt tagtatttca
      961 ggaagtattt ggctcatcta taaaattttt caaacaagat attcattaaa aggctctaaa
     1021 attcgattta agcgattttt ctttcttggc tgtatatttt ccttgataat cgtttcaagc
     1081 ggcttattcg gcgttttaga gggaaataag cgcgtttcta gaagtatttt attaggaaat
     1141 gtcactcaaa agtatgaatc agcgcgtgat aaaaagaaaa aagagcaagc attagcacaa
     1201 aaaatggaag agtttaccac ttgttataag gaaatgaacg atatttttgt gaatcaagaa
     1261 aaacgtttga cggataaaaa catggaaaaa ctcactcgtc tttatcaaaa cttgccagaa
     1321 aaacagcaaa aagaggttca agacaactat gagcgaacca aaaaagacgt gcaatatgta
     1381 aaagatacaa agatagaaga agcctgtagt gatctatttg gagacacaaa cccttggttt
     1441 gccagtgaag aagaaaaaaa agaaaaacaa caatctgtca catatgaacg ctacgaaaac
     1501 ctgtttcaac aagcaactaa catgcaaaat ccgacaaaaa aagaaaccgc cttaaactat
     1561 ctaaaaagtg tcaaggaatg gttagatcag caacagcaaa attgaatttg attgaaactc
     1621 atgttaaact gaatgtgcaa atgatcaaac attagataca cgaaagcccc agagaggtcg
     1681 gattctctgg ggcttttttt caggttgatg tcgcctttac tgattttact tcttgtttcg
     1741 gttattcaac caataactga ataaagagac acaaactcct acaagaaggg ggcaaatgaa
     1801 ctgttcaaac attagataca cctccttcca gtcgcaagac tgtcggatag gcgacaagtt
     1861 attataccct acaaaattaa atctgtgtgg cgattttaag tataccttat tttcgggttg
     1921 ttagggcttg ccctgaccgc ctgtaaggcg cttctcgact agattaaggg agttaatcaa
     1981 gaattgtctt agaacggctt agacaagctc tcagattctc ccacctcctc tttgaacaac
     2041 ggttttagca aaggagccgg aggcgactgc gcaggtacaa tgtgagagcg tagcgatcac
     2101 acctttttag cgaccgtagg gagcaagttt tctagtttag aaaaattcgc gaaccttttt
     2161 ggaggtaaaa gtaggagttt tttaggcggg aaattaaatg tgctcttaag atatactgtt
     2221 agatatactg ttagatatac tgttaataat aactgttagg gattagcaac cttatgggag
     2281 agtaagtatt gacataggta gtgggggata aattgtcaca ctatggggga taaattgtca
     2341 cactatgggg gataaattgt cacactatgg gggataaatt gtcacgctat gggggataaa
     2401 ttgtatttta caccctctta tgtctatgat aacataattt attgaggtga tttttattga
     2461 gtaacgatat agttcgatat aataatggat taaattctgt ccctttaaga aaatttaccc
     2521 ctatagatat ggatttattt tggggaatat gttctaaaat gaaaagaaaa ggtacaaaag
     2581 aagaaatttt tacatttaaa caaataaaag aattaattga ttacaatata aatgcacgtg
     2641 gcgaccgttt tgctaatgat ttaataaaaa tgtcaaaaaa aatcattact ttgtctttta
     2701 tttatgaaga cgaaaaaaca tatgaacaat taaatctgtt tcaaaaattt aaaatcgata
     2761 aagtgaatga aacattatta ataaaggttt ctgaagaatt tgaatatata cttaattcaa
     2821 tcggaactaa ttttactcgt ttcgaattag aaaatatgac aaacttaaag agtagctacg
     2881 ttaaagagtt atatcgccaa cttatggctc ataaagacag aagatctaaa aaaggtcact
     2941 ggtttgtaaa aatagaagat tttagagctc tattagcaat accagaaagt tatcgcatga
     3001 gagatattga taaacaaatt tttgcccaag cgaaaaaaga atttttaaac gttaattcaa
     3061 ctcaccctgc tatttttaaa acttttgaaa tcaaaaaaat taaagcctac aaaggaaata
     3121 agataggatc tctgcaaatt tattttgaag aaaatatacc ttcaattacc ttagataatt
     3181 ggttagagaa ataataacct aacaatttag gaggcaatta tcatgcaacc caaagaaacc
     3241 tttacaataa aggaattatc tgaattattt gaaataagta ggcaagcaat ttctaaacat
     3301 gttgataaat tagacccgtc cttattagct aaaaatgagc gtggttataa ggttgtatta
     3361 cgttctggag tattgcaact tgctagcaac ttaggtaacc aaaggttact agataggttg
     3421 catgagtatg aacaagaaaa cgataaaaac gtttatatat caagaaaaaa cgtggttgta
     3481 aacgagttgc tagataagtt gcgtgaagaa aatgaatttt tacgagaaca actttctctc
     3541 aaagagcaaa ataattctaa tatgcaaaaa ttactcgacc agcaacaaca actcaccttg
     3601 caggcaaatc aacagatcga aaaactgcaa gaacagttac aattagctta tacggaagaa
     3661 cctgaagaag aggtattaac ttctgtcaat aaagaaaaca acaatgcaat aacaacagaa
     3721 aaaaagtggt ggaaattttg gtaacaatta ccaactttcc ataaagaaga aatcaatttc
     3781 tatagacttt atctttctaa aaataaaaaa tatgtttagg tgatcaaatg actataagta
     3841 aagtaatcaa acaaaaaaga atagaaaaac aactaactca agaagattta gctgaaatgc
     3901 ttttagtatc taaaaaaacc atttctaatt gggaaaatgg tagaacaata ccagatacag
     3961 aaaatttaat tcaatttgct aagttatttg atttatcact agacaatatt ttgttaggag
     4021 aagaaaaagt tatgttaaaa aatcatacaa aaacactaat actattattt cattttgtaa
     4081 tttttttaag tttagttttt accttaattc agtccaaatt cttttatttt agccattcaa
     4141 ttgccacttg gttattaata ttttttctaa gtttgctatt agtaatcaat actctgatta
     4201 ttatttttaa taaacataaa aatttatttc ttcatagtgt ttttccaatt attttgctga
     4261 gttttttata ctttgtataa agtataaaaa aagactgctc tttcgagcag ccagttaaaa
     4321 aaataatgag agtttattct tgaaaaagca acgcgcttat aaagcgctac caaaatttta
     4381 tcatatttta taaaaaataa caatccagta aaaaatgact ggtaaaaatt cacaaagaaa
     4441 cggaaagaag caactgcaga aaatcaagcc ttaaaaaaag cttttgacct agaccaatga
     4501 aaaatcacaa agtgagcagc tatagccctt gatagagggg ctacttggca tagtcgacat
     4561 tatgttaact aacgctataa ataacgatta tgttcccaaa acaatatttt atgaactata
     4621 acaaaaattt gttatcgcat attgtatatt ttaattatag gtgtatacca cttaaattta
     4681 acacaaaggc gtgattattt tgaaacccaa aaagaaaatt tatctaggtc tcctcttttc
     4741 cttgatgatt ggaattttta gtataggaat tacaaatacc tatgcttcag aaaattctaa
     4801 tttcaatatc aactcccaac caattgaaga acaagcaatt aattttgcga aaactgattt
     4861 tgaacactcc ataacttcaa ttcttttaat ggaaaataac accgaaactg atattaacta
     4921 tactcttggt tcgccattta aaatgctcga tggagaaaat acggcaaata gttctatttt
     4981 ctttccagta ttttcagacg ctgataatac gattaaatat atttataccg tcaaaaaaat
     5041 acaaatggag aatattcagg aagtatttca caatttttag caaaagaact tcaaaatcta
     5101 gtagatagta atataacaga tacaacctta ttttttcaag ataatagcat ttattattcc
     5161 gataatggac aaattaaact actttggaaa tcacctttaa gtacagaaaa aaatggtgtt
     5221 tcttccttta cagaaaaaaa tgatcttata tccgtaaata taaaaaataa aattaaaaat
     5281 ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa aaagtgtctc ctccggagat
     5341 aactttatta tgattaattg gcgtatagca gaaacccaaa caagttttcc gtggtgtgct
     5401 gcttacgtag aagctgctat cttatgtaac aaaacagatg tacgtgctac ttcagctaga
     5461 gccattatgc attataccta tcctaattta aacaacagtc aaattgaatc aaaaagtctt
     5521 aatcaagatc aaataataaa atatgcaaat tactgtggtg tttatcctaa aaaagtagca
     5581 cgaatactaa acctttcaga ggtccaaaca caactaagaa aaggaaatgc catttatatg
     5641 ggaactgctg gttatggaaa ttatagtaac agccgtcatg cacttgcatt atttgggtgg
     5701 gtcaatcaag gtggttatca aacttactat gtgtggaatc cttggtataa ctacccaact
     5761 gtaataagtg caaatgcagg accagttaca attcctgtac ctggaggtgg ttttacttgg
     5821 gacaatacca ttacaaattg gtaaataaac ccattgcttt agttatgggt ctagtgttag
     5881 ttcttttctt aactagttgt tcaaaaaaac aagaaataca aaagactgat ctgattcctt
     5941 ttctaggaac ttggcaaact aaagatcatt cagaacgtct agtaatcact aatttaaata
     6001 cttcaaaaaa tatagaacaa tataccatct tagtaaaaga gaacaatcat aaaattcaaa
     6061 tcattgataa aaatttaaat ataaatgaac aagttttcat ttacaaagca acaaataatt
     6121 tactaacact gcgctctaat aaaaaacaat tagattacac ttttagtttg gattctccaa
     6181 aaaagctcgt atttattttg acaacgaatg atgggacaga agggacagct aaaccaattg
     6241 aattttttaa agctgattga tgaatagttg tttgatatta caaaacaata atttcaccga
     6301 aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt tgctagcctt ttttgtattg
     6361 tggagcgtgg gttttgaggg ggattcaagg gggagcttgc gcccctttga aaagcggtta
     6421 tttgctagcc aaatacgtag gtatttggct agcaaatata gagcttgcca aaccctttta
     6481 gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat acgtccgtag
     6541 gacgtactga aagggtggga cttatgagcg aacaaaatca aaatttggct agcgatcatt
     6601 ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg agcgaatctg
     6661 aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg gctttcgtaa
     6721 aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac attgcccagg
     6781 caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgct aagtggtgta
     6841 atacacacca acacgacgtt gctcctggag acgcccaacg cttatccgaa aacctaaaaa
     6901 tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagccgaa ccacttcttg
     6961 tagtcgtgca attaattacg ctgaaccacg agcaaccatt aaaacaggaa taaactgtga
     7021 cgtcgattat gccaaaagtg aaatgaaaca aattcgcatg ctgtatggaa aagacgaccg
     7081 tgtacaggca cacttattga ttcaatcttt tcgaccagga gaaattaccg ctgaaaaagc
     7141 taatcaacta ggggcagagt atgc
//
LOCUS       DAZKCZ010000106         6947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000106 DAZKCZ010000000
VERSION     DAZKCZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.109"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..855
                     /locus_tag="ACG8KN_002219"
     CDS             259..855
                     /locus_tag="ACG8KN_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9189234.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(913..1395)
                     /locus_tag="ACG8KN_002220"
     CDS             complement(913..1395)
                     /locus_tag="ACG8KN_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189235.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1501..1698)
                     /locus_tag="ACG8KN_002221"
     CDS             complement(1501..1698)
                     /locus_tag="ACG8KN_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189236.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            1993..2751
                     /locus_tag="ACG8KN_002222"
     CDS             1993..2751
                     /locus_tag="ACG8KN_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFP9189237.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2811..3239
                     /locus_tag="ACG8KN_002223"
     CDS             2811..3239
                     /locus_tag="ACG8KN_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189238.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3285..3357
                     /locus_tag="ACG8KN_002224"
     tRNA            3285..3357
                     /locus_tag="ACG8KN_002224"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3318..3320,aa:Gln,seq:ctg)
     gene            complement(3507..3878)
                     /locus_tag="ACG8KN_002225"
     CDS             complement(3507..3878)
                     /locus_tag="ACG8KN_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9189239.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4093..4278
                     /locus_tag="ACG8KN_002226"
     CDS             4093..4278
                     /locus_tag="ACG8KN_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189240.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4361..4549)
                     /locus_tag="ACG8KN_002227"
     CDS             complement(4361..4549)
                     /locus_tag="ACG8KN_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189241.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4573..4836)
                     /locus_tag="ACG8KN_002228"
     CDS             complement(4573..4836)
                     /locus_tag="ACG8KN_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189242.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            4994..5251
                     /locus_tag="ACG8KN_002229"
     CDS             4994..5251
                     /locus_tag="ACG8KN_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFP9189243.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5322..5513)
                     /locus_tag="ACG8KN_002230"
     CDS             complement(5322..5513)
                     /locus_tag="ACG8KN_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189244.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5654..5974)
                     /locus_tag="ACG8KN_002231"
     CDS             complement(5654..5974)
                     /locus_tag="ACG8KN_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189245.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(5997..6431)
                     /locus_tag="ACG8KN_002232"
     CDS             complement(5997..6431)
                     /locus_tag="ACG8KN_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9189246.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6766..>6947
                     /locus_tag="ACG8KN_002233"
     CDS             6766..>6947
                     /locus_tag="ACG8KN_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9189247.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2171 a         1358 c         1066 g         2352 t
ORIGIN      
        1 tcataaaaaa aagaagatag agatttaata attatatatc agctgttttt ttcaaataag
       61 caaaaattcc taaaacacaa caatgaaagc gataactaag aaaatcctta cataacgatt
      121 tttcttatta aaattgtttt ttgaacttcg cttatttttt gtgagcatat ctgtttactt
      181 actttttgta aggtgctata cttcatttcg taatcaatta aaaaaatgca aaacgcaaat
      241 caaaggagaa attttattat gacaactttt actgacactt tgaaaaaccg ccgttcaatc
      301 tatcatctag gacgtaatgt ttcactttct aatgaagaat taacaacttt gatcaaagaa
      361 gcaattaaag aaagcccaac tgctttcaat gctcaatcaa cacgcgcagt tatccttttc
      421 ggagatgcac atgaaaaact ttgggaaatc actgaagaag cacttcgccc attgactcca
      481 gcagaagctt tcccaaatac acaaaacaaa ttagctggtt tcaaaaatgg ttatggtaca
      541 gtattattct tcaaagatac tgacgtggta aaaggtctac aagaacaatt tgaattatat
      601 gcagacaact tcccagattg gtcagaacaa tcaaacggaa tcgcaacagc taatacttgg
      661 gtagcattag ttgataaagg attgggcgca aacttgcaac attacaaccc agttattgac
      721 gaagcagttg caaaagaatg gaatatccct tcaaactgga aactacgttc gcaattagta
      781 ttcggttcac cagaaacacc ggctggggaa aaagaataca tgaacgatgc cgatcgcttc
      841 cgcgtatttg gataatttta tttgaaataa aaaacagagg ttgtgacaaa catcttgtcg
      901 caacctctgt ttttattctg gatcaatcat aatatcaatc tctgcctcat cgtttaagcg
      961 ttgggacaaa aatggaacta attcttctaa tgatgcagaa aatttcagca tcggtcctcg
     1021 ccatccgttt cggaaaaaat tcatttttac gattttagaa ccttttggct gatataatac
     1081 aattttattg atctcagaaa aaggaacacc ttttttgtca aagctattca aaacaagtcc
     1141 ttcttctgtg attcctctac tgtccaacaa aaaactaata aaaatcattg ctgacaagat
     1201 cccttttgcc aattcatctg ctgtaggatg actgatgagt gtataccaaa tgactagtga
     1261 cactaagaag ggtatcacca agtagaccca tcgatttcgc acagtccgat aaataatttc
     1321 ttttcgaacc agccaaagaa ataaaaacat cacaacaacg aataaaaaca atagaaccgt
     1381 aaagaatgtt accatgacaa ctcgcctcgc tttttcttct ccagaaaata ggaaaagaat
     1441 actgatttac cttctcaaag aaattggact tgcagcacgc cgcacgtcca attgttttct
     1501 ttattcaaat gtatcatatt ttgcaacaac catgcttgct ttttcatcta ataattcttg
     1561 gtcctctgca gcacattggg caactttgac cacggcgcag tttgtcattt ttgatacaac
     1621 ttcaccaatg accgtttttg taaaaccaat tggtttgcat gcatattgtt ctcctaaatt
     1681 aatcatttct tccgtcatca aaatcctcct aaactgctgg aacttttctc cacattacca
     1741 ctgctatttc tttggagatt taactcattg atttttattt acagttaaaa atcaacctta
     1801 caggcattat agcgtactgt tttcgttttc acaattaaaa cagctgttgt atttagaaga
     1861 atttttttca aaaaattaaa acatattcct taaaaactaa ttatttccta catattaaaa
     1921 tataatactt ccatttccaa taaaaataca tttataatag gaaagataaa cagctgaaaa
     1981 gggagatttc aaatgaagaa aaaaacgttc aaaaaactat tgtttctagg gggcacagct
     2041 gctcttttat taagcggtac aagttattac ctacatatcc attctccaga acatacaatg
     2101 aacactgcac aaaaaagttc aacacaaaaa acgaaaaaga caaaagagaa agcagaagtt
     2161 ttactagatg ttccattaga gagccagttt gattctcctt ctttagaaaa tggctgtgaa
     2221 gtcacttcgc tttctatgct tttatctttt tatggttatg aaacaactaa aaaccagtta
     2281 gctgagcaat tggattacgt ccctgttttc aatgcagatg ggactcatgg cgatcctaat
     2341 gaaggatttg ttggtgatat cagtggcgga gactgggcta tggtcgtcta tgttccccca
     2401 gttgcctccc ttgctcagca aatcgtccaa acggattatc acacttttcc aaaaaccgat
     2461 gctcaactcg atgatatcaa aaaagcacta tctaaaggaa atcctgtctg gacttctgtg
     2521 actatcaatt ttgaagtccc agatgaaact gattttatga cttggaccac aaataatgga
     2581 gaagtaagag tcactcctct tgttcatgct tgtgtagtca ctggatatga caaagaaaat
     2641 atttatgtca acgatcccta tggtgtaaaa aatcgagcag taccgatcaa cgatttttcg
     2701 gcgattttca ctgcaatggg tggacagatg ctaactttgg agaaaagcta gtaattcaaa
     2761 caaaaatccc ttatactcaa gataccaaac ctttgaaggg agcgtagata atggaaaaat
     2821 tcacacataa aaaaatggat ccaaatgaaa ttccgattat ttttgtccgt gactgcaagg
     2881 gaaatgttca aggtaaagtc tctattaatg agtggaacga gcgtcgcaga cctgctaccc
     2941 tcaatgaact ggaaatcaaa ttgtatcgtc aatctcttgt ttattacgca gatcaagagt
     3001 acgagaaagc caccgattta ttgaaatttc taattgctcg aacagagtat actcgctttg
     3061 aatatattga acgtttagca aacatctatc atattatgaa tgaacctgtc aaagaatatc
     3121 agttattgga caccgtcttg tcagtagcag aactgattgc ccttcctgct ggattagaaa
     3181 agaaactagt acgccgcctt ttacgagtaa agcaacaact atccgatcaa gaaaaataac
     3241 ttgcgttttt ttaggaaagt gctatgatta ttttgttgaa taaatgggat gtagccaaac
     3301 tggtaaggca cttgactctg aatcaagcaa ttgtaggttc gaaccctgcc atcccaattt
     3361 gatgtgcttg ctgaaaaaat gaaccccctg taaaagaaca atctttttgc agggggatta
     3421 ttgtattatt tttactactt gtatttagtt tacataatta aattatgaat aataaaatcg
     3481 ggcgtattct taatacaaaa actgatttac ccaataattg ctaatgcttt ctccggaact
     3541 ttttctctag gaacttcctc atacgtttct atgtgtgctc ctttattgct aattttcaaa
     3601 taatgatttt cttttaacac agaaagacca ttaaattcaa ccgttcgttc tttcccattg
     3661 gcatcagcag ctttctgcac ataccttgct gtaccatatt catttacttc ctctggtttt
     3721 tgtgtcttta catagacctc tcctttttca actaatggat ttagctgatc aagtatacca
     3781 gggatttctc cataacttcc ttcagtataa tatttcagtc caaatagcaa aataccacta
     3841 atcgccgcca gtccaatcaa atatttaatc actttcatct ttctacctcc ttgttatata
     3901 ttcagtatat gggtcagtta aagaaattcc atccgtccca agttggagat ttttctccta
     3961 ctttagaagc gagaatcgag tgttttttta gtaaaaatga tcgttatttg atattattga
     4021 cgtgtggata aatcagtttt tctcgttttg tccatgtaaa aaatcatgtt gatagcaaag
     4081 gatgtgttca aaatgaatca agaagaatta tttcaaaaag tcaaggaaat gattaagaac
     4141 ggcaatttcg atggcgcaaa acgatttatc gaagaacata aagaacaatt aggaccttat
     4201 aaagagaaag cccaaaatct tttaaaagat gtaaatatcg acagtgtcaa aaacaaattt
     4261 aaaaatctat tcaaataagt aaaactttca cttttgtaaa atcaaaggag gacaatgctg
     4321 ctgcatcgtc ctcctttggt tcttttctga atacttcata ctaaaataaa cgtgtcggat
     4381 cgtaatgata agtcacattg ccgttcagtg aggcttttcc caccacatat ttacgattct
     4441 ccaacttcag ccacgcttct cgaaaagcca ttaattcttc tctttttaca tcaatctttt
     4501 cgatttttcc atcgatcaaa tcttgaataa gttgttcata tggattcatt tttttcctcc
     4561 atctgctgtc aatcaataag ttgtaaaaat aggttcatat gcttttttgg tatctagatc
     4621 ttctctgact ttgactgtcg tataaataat tttttctact gggatactat aataacttcc
     4681 ttcaccatta ttgaatcggt gaacgtcttt ctttgctaaa ataccattta ttttattcgt
     4741 cagtacacgt tgttcgacac tgttacttac aacatagtaa atcacccgaa tattattgat
     4801 caaatgcaaa tcgatttcta acactacatg atccatgaaa atcccactcc ttctttcact
     4861 ttgattatag caagtttctc ctctaatttc aaaatcgata cagattttac cggataaatc
     4921 cacaaaactg cttgcgccat ttcgcacaaa gtgttatttt gtatcatgga ctttatttat
     4981 cggaggcaat agtatgatcg cgatcggtca atttgttttt tatattcctt tttttattat
     5041 gataagcatt cttttctatt acataaaatg gacaaaaaag aagttttcag ttttactggc
     5101 ctctttacct gctgtatatt ttacttatca aattttttct tttaggcatt gggaaacaac
     5161 gtctgtatta ataacacaca tcatcgaatt gacgctttca gtaatttttc ttattatttg
     5221 gatctatttt ttatataaga atcagaattg aatccctcgc atgtttaaaa agaaaacgat
     5281 taaagatgca gcaaatttct gccgatcttt aatcgttttt tctattctgt tataccaaaa
     5341 aaatgtttga atcggtgaag gatcgttcct tgttttaatt ctataaattc ttgtgcgatc
     5401 gggtactttt cttccaaatt ttctttgttc ttttcgatca ggcttttcgc ttcttcatat
     5461 tttttatctg cacatagtgc ttttacttcc tgtaatactt ttcctgcttc catttgcctc
     5521 accctcttgc cattttattg ctttaagttt tatttaagtt atacgcatta ttctagtttc
     5581 atcccaaata accttttttc atttttactc ctcgaactaa tcagttttct aaaggctgat
     5641 tggttcgtct tttttatttt tcctccattt cattctgctc ttctgttaat ttatctaatg
     5701 ctttgattga tgctgcggct cttgcataat catagctgta taattccata aatttatcag
     5761 gcagaacaaa ccacgctgga tgataaccgt tagctgtgac agggaatcct ttatcttgtt
     5821 ggttatctcc atcccatctc agagcaacac ctttttttcc tttccacttt actaaagcga
     5881 tcgagtatcc cttctttgtt tgtgtgccgt cttcaataat tgctaaaatt tctatttttt
     5941 cttttggcgc acggacttct tgaggacgaa ccattttcta ttcctccctt atctcattac
     6001 ttcacgacca acacactgca aggggcatgt gatacgacat aagaagcggt cgatccaact
     6061 aatgcttgtt gtatagcacc ttttcctgtc gctcccataa cgatcaaatc aatattttct
     6121 tgtccaggga tgacattggc aatcaaattt cttggatctc cagtttcaac tattgcgtgg
     6181 atctcagcaa tgccgtactc tttggcatca ttgatttttt tcagcatggc tacttctgtt
     6241 ctttgttttt catctgcata gatttttgaa aaggcatagg cactatgggt cagctcaact
     6301 ttgttgatga ttgacacgat aaataagtgg gcttcattgt cctttgctaa gtcaagggct
     6361 tcagcgaacg ctttgatcga ttgtcttgat ccgtcaactg ctaccatgat gttctggtat
     6421 cttttactca ttggaaccgc ctcctttttc ttttattgta acatctcaag caaaaataca
     6481 aaaaatatac gcaatatgta aataaaatga tgaacaaaag caatttcttt caagttattt
     6541 atacatttct tttctcatca gaacacctgt tctaatccca ttatttctgc attatgtgaa
     6601 attcctcatc atttttcaca tcgagcgaac atcattctgt tacaaaaaac gtggatttgt
     6661 cataaattgt catgcttctt ttacctatga aatattttca cgtgttaaag tattcctcgt
     6721 tgaacacaac aaataaaact ttttaacgaa taaaggagat ttttcatgac atcacttaaa
     6781 actttacttt ttggaacaac tttggctgcc ggcgctgcat tcttcatggg aacgactgct
     6841 catgcggacg aagcttatac cgttcaatca ggtgatactc tatcaacgat ttctcaaaaa
     6901 tacgttggtg ataactcctt gatcaatgcc attgcagaat caaattc
//
LOCUS       DAZKCZ010000107         6830 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000107 DAZKCZ010000000
VERSION     DAZKCZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.110"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            251..466
                     /locus_tag="ACG8KN_002234"
                     /pseudo
     CDS             251..466
                     /locus_tag="ACG8KN_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            604..1575
                     /locus_tag="ACG8KN_002235"
     CDS             604..1575
                     /locus_tag="ACG8KN_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9189248.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            1716..3029
                     /locus_tag="ACG8KN_002236"
     CDS             1716..3029
                     /locus_tag="ACG8KN_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFP9189249.1"
                     /translation="MKRRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNDKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIISLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3072..3938
                     /locus_tag="ACG8KN_002237"
     CDS             3072..3938
                     /locus_tag="ACG8KN_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9189250.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            3935..4783
                     /locus_tag="ACG8KN_002238"
     CDS             3935..4783
                     /locus_tag="ACG8KN_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9189251.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            4797..6269
                     /locus_tag="ACG8KN_002239"
     CDS             4797..6269
                     /locus_tag="ACG8KN_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFP9189252.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWINDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMTAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKGLVYDQHYLRLEINLKQATKLELHYAKGPHHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6286..>6830
                     /locus_tag="ACG8KN_002240"
     CDS             6286..>6830
                     /locus_tag="ACG8KN_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9189253.1"
                     /translation="MIYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMA"
BASE COUNT         2097 a         1182 c         1487 g         2064 t
ORIGIN      
        1 ttaattttgt tggcaaatat agtttccgct atgaaaaaga attatcgaat ggaatactac
       61 gtccgttaaa tatagagaat tattgagaat gttcgtcttt tggttcctac cgtgcatttt
      121 cgcacggatg ttccagatac tatttttgaa tgtataaaat tgaaaacaca agaatcctat
      181 taaatcaacg attcttgtgt ttttaatttt tcactaccgt gcattttcgt ttctatgttc
      241 catgtacccc aatcttgaaa aattaattaa agacttatat aaagccattg acgacttagt
      301 cgtggtgcta ccggagtatc aatgtttaac cagtatccca ggtattggtc ctgtttaggc
      361 tgctggaata ctcgctgaaa ttggtccaat tgaacgtttt tctgatgaaa taaaattgac
      421 aaagtatgcc ggcctatatt ggcgacagag tcaatccggt aaatctgaat acgaaaattt
      481 ttttacgaaa agcttcacgt ctaatatcta ttttaaaata aaaatattcc tgctaatttg
      541 gaatcgtttc cttcttttgg tataataata cctgaaattc ttttgagagg agttgttcgt
      601 gaagtgaaaa ctactatgaa ggatgttgct gcgttagcag gagttggtgt gggtacggta
      661 tctagagtaa taaatggagt aaaggtgaaa gaggttacca aagaaaaagt aatggctgca
      721 attagtgagc ttaattatga gcctgatgaa tatgccagag gtatgaaaac gaatagcagc
      781 aatacaatag ctcttatttt acctacgatt tggcatccct tcttttccga gtttgcctac
      841 tatgtagagg aagctctcag ccaaaaaaat tataagcttt ttttgtgtaa tgcgaacggc
      901 agtgcggaaa aagaaaaaga atatatccaa atggtcaagc agagtaaagt agacggaatt
      961 ataggtatta cctattcaga cattgataaa tatgtatcat caaatttacc atttgtcagt
     1021 atagaccgcc atttttcgga acaggtttct tatgtaacgg cagataatta tttaggtggg
     1081 caaattgccg ctcaagaact acttactcgt aactgtaaaa acttgtgcta tgttgggggt
     1141 gtttcacctt accctaacga aacaaacaat cgtaaagctg gtttttacaa tatttgtgag
     1201 gaaaataaaa tagctgtgaa ggtattggat atgcctgaac caattagcaa tttggaggaa
     1261 caattgctcg cttttttttc ggataatcct gaaattgata gcatttttac catcaatgat
     1321 ttcatggctt tggatgtgat aaaagcccaa aaaaaactag ggaaggaagc acctaaggat
     1381 tttcaaatta ttggttttga tggagtcaag ctatcgttgc aaaaagacta tttactatca
     1441 acgattgtac agccgataga ggaaatggcc aaggtttcag tagaaacact catggcgatg
     1501 attttaaatg aagaaccgcc taagcgtgtg gtagtaccgg ttttctttca cgctggagaa
     1561 acgactaaag agtaaaaaac cggtttataa cgattttttt tgagataggt tgacagaaag
     1621 tggtttcaat gatatcttaa aaccaattaa ttggaaacgt taccaattaa ttggaaaata
     1681 aaacggaaac gtttcaaaaa ataggaggaa catcgatgaa aaggagaatg ttagcaattg
     1741 cagcaatttt agcattaaca gctggaggac ttgcaggatg tggcagtaag gagaaaagtt
     1801 caggcagcag tgattcagga gaaattaaga tttgggttca gttttctgat gaaacgcctg
     1861 aaggaaaagc ttgggcagag attgtgaaca attttaatga tgaatacgag ggtgaatata
     1921 aagctgttac tgagtatatt cctcgtagcg gaagcggcgg tggatatgag gataaagtaa
     1981 atgctgcaat tacaaccaat acccttcctg atgttattac gttagatgga ccgaatactg
     2041 cagcttatgc tcattcagga gtcattgctc cattagatga ttatttgaaa gatgcggaca
     2101 tggatgatgt attagacagc attattcagc aaggaaccta cgatggcaaa atgtatgcgt
     2161 ttggtttctc agagtccagt gtgggtgtct attacaataa gtcgttgttc aaagatgctg
     2221 gaattacaga agatcaattg ccgacgttag aagatccttg gacttggacg gaatttcaag
     2281 caatttgcca aaagctgact gataaataca aaacgcctgc aattgatatg caaatggcgt
     2341 cgggagacga aatgctgacc tacgcatata caccatttgt atggtcgaat ggcggcgatg
     2401 tcgttaatgc tgatggaacc aaagccgaag actattttaa cgataaaaaa tccgttgaag
     2461 cgttggaatt tattcaagaa ttggttgaaa aaggttacac cacaatcagt cctattgaaa
     2521 aaggatttga aactggcaaa tatgccatgc tgctgagcgg ttcttggaca atggcagatt
     2581 tagaggaaaa ctatcaagag attgattatg gtattttgcc ttaccccgtt tcagatgcaa
     2641 ctggggagtt agtttcacca accggcagct ggcagttagc aatgactgag aaaacagaga
     2701 aaaaggatgc ttctgcggcc tttattaagt ttgcaaccag tacagaatct agtcgtatta
     2761 ttagtttagg gaacagtgtt ctacctatca gaaattcaac agttgattta attaaagatg
     2821 aagtgtcgga aggaatgcag gtattaatta agcaaaacca aatttctgga catgcccgcc
     2881 cagtggttgt agcttatccg caagtatcta gagcattcca acaagcagtt caagatatta
     2941 gctacttcga ggaaaaccca gatgtacaaa aagtagcaga tagtcgcgct gctgaaatgc
     3001 aacaggcaat tgatacttca ctaaaataga agtttctaaa gggggccaat caagtgttaa
     3061 aaaaaccgac aatgaaagac aatttgatag gttacggctt tctagcgcca gctttgctgc
     3121 tgctgacgat ttttctgatc atacccgtaa tcatggtaat ttattatgcc tttactgatt
     3181 attatttgtt aacgccagat gctagacaat ttatcggctt agaaaatttt aagcgactgt
     3241 tcgctgatcc aatttttgta aaaagtattt ggaacacggc taaatttgtt gtttggatta
     3301 ttccggtcca attaggggca gctttaggaa tggcacttat tatcaacaaa aatcgtaaag
     3361 gaaatatctt ttttaaagta gccttttttg cgccagtcgt gatgtcctta gtcgttattt
     3421 cgattttatg gttgtacttg ttaaatccta atgaaggtct ggttaatacg ctatttgcaa
     3481 agattggaat tgcttcgcag ccttttttga ctagtcccaa acaagcaatg tatacgcttg
     3541 ttttcgtatc ggcatggcaa ggtgcaggat atcagatgct gctgttttta gggggaatgc
     3601 aaaatattcc gcaagacgtt tatgaggcag ccgaaattga tggtttttct aagtttcagc
     3661 aatttcgcta tatcaccatg ccattattaa agcccacggc gatttttatt ttacttacca
     3721 cattgatttc ggcctttaaa ctaatcattc agccaatggt aatgactcaa ggtggaccaa
     3781 tgaattctac tatgacaatg gtttattaca tttaccaaac tggatttaca gaccgtatgg
     3841 taggctactc cagctccatt gcgttaatct ttaccaccat gattggttta attacaattg
     3901 ctcaaagaaa attggtaaag gaggatgatc cagcatgacc aagaaatttg gaccgctaat
     3961 gatcgtagaa tattgtttgc tgttcctttt agcggcatta ttcattttcc caatgctgtg
     4021 gatggtagtt tcctctatga agcctgaagc agatgtctat caaaatttag cttccttcaa
     4081 agctttttta cctagtttga atccagctaa ttggtttaaa acttatcaag aagttatttc
     4141 ccggttcagt gtgggaactt atttattaaa cagtattttt tatggtttaa cctttgcctg
     4201 tgggtcaatc attgtaaact ctttggcggg ttttgccttt gcgaaggtta atttttcagg
     4261 caagaaaatt atctttggag ctttgttagc tctgctgatt gtcccgatgg agacggtttt
     4321 aattccgcaa tttaccatcg ttaattcact aggcttagtg aatactcgct tggcagtgat
     4381 tttaccagcg atggccagcg cgtttaatat ctatttgttt agaaatttct ttatcgcgat
     4441 tccagaagaa atcattgaat cagcgaaatt agatggtgca aatattgtta aaattttctt
     4501 tcacatcatg ctgccaatgt ctaaaccagc catagccaca gtaggtgtct tatcctttat
     4561 ctccagctgg aatgattata tttggccgtt aatggtctta actgacagca gcaaattttc
     4621 aatgcaagtg gcaattacta cgatcaatac tacacagcca gtttatatca atcaagtgat
     4681 ggctgtttta accattgcaa cgattccact gattattgtt tatatcgttg cacaaaaata
     4741 tattctgcag ggacttggcg gttctggaac gggtataaaa taggagtgaa tagtgaatgt
     4801 tgcaaaaact tttaaaggcc aatgagttta ttgagaaaaa tgctcaaaca gtcaatcaag
     4861 aataccggaa taaatttcat ttgatggctc cgattggctg gatcaatgat ccaaatggtt
     4921 ttgtttatta tcaagaggag tatcacttgt tttatcaata ttatccttac gatagcatct
     4981 ggggaccaat gcattggggc catgctaaaa gcaaggatct aattcactgg gagcatttgc
     5041 cagtagcact ggcaccaggt gaaagctatg acaaggaagg ctgtttttcc ggcagtgcca
     5101 tagaaaagga tggcaagctg tatttgattt atacaggaca caatgttgtg gaagggcaag
     5161 ttcgacaagt tcagtgtttg gcagtttcag aagatggcat tcactttgaa aaatatgccg
     5221 gaaatccaat catcgcagaa gatcacttag cttctgttgc aacgaccgaa gatttccgcg
     5281 atcccaaagt gtttcagcga aaagaagact attttactgt agtcgctact aaaactgcag
     5341 atgctcgtgg acaaattgtg atgttccatt ccaaagattt actaacttgg gagttttatt
     5401 ccgtattgct ggagggaact ccagaacaag gcattgtatg ggaatgccca gacttgtttg
     5461 aactagatgg caaagaagtc ttgattcttt ctcctattca aatgactcca caaggcaatg
     5521 cttaccaaaa tacgaactca actgttgctt tcattggtac tgtcgattgg caaacaggca
     5581 aatttctagt agagaatttt catgaaatcg atggcggttt agatttttat gccccccaga
     5641 cctgtgaaaa cgaagcaggc caaagaataa tgactgcttg gatgcaaatg tggcagcgta
     5701 aaatacctac gaatgatctg ggccatggtt ggtcaggttc catgaccttg cctagagagc
     5761 tgagggtggc tgataatcaa ttgttgcaac ggccagtttc atcaatttat caagtaattg
     5821 atccgcagga aaggattgaa tgggcgaaga ctgaagagaa tcgattctta ctgaagggct
     5881 tagtatatga ccagcactat ttacgcttgg aaatcaattt aaaacaggca acgaaactag
     5941 aacttcacta tgcgaaggga ccccaccata cattactgct taactatgat gttgaacggg
     6001 agtgctttac tgcttccagg gaacacatgc cgtatgattt atcaggagct gaaaaggaag
     6061 tgttgaaaaa gcgcagtgtg acgattcctt tggaaaataa ttgtctaacc atcgaaattt
     6121 tccgtgatac ttcagctatt gagctttttc taaatgatcg tctgactatg agtacaactt
     6181 tttatgagac agaaaaagga caggattttc ttttgctggc agacagtgaa gtaacctttt
     6241 cattggaaag cggcttaatt aatctataga agggagagct cagtgatgat ttatggtgca
     6301 attgaagcag gcggcacaaa atttgtgtgt gccattggcg atgaagaaat gacgatcaag
     6361 gaaagagtga gctttccgac aacgacacct gaggaaacaa tgcctttagt cattgacttc
     6421 ttcaaacaat accaagcaga tttagcaggg attgggattg gctcatttgg gcctatcgat
     6481 atccatcgcg attccgccac ttatgggtac attacctcga ccccgaaatt ggcatggcaa
     6541 aattttgatt tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca
     6601 gatgtcaatg cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt
     6661 gtcgtttact atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt
     6721 atcgaagggt ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat
     6781 gactttgctg gcagctgtcc atttcatggc aactgtttag aagggatggc
//
LOCUS       DAZKCZ010000108         6816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000108 DAZKCZ010000000
VERSION     DAZKCZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.111"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..602)
                     /locus_tag="ACG8KN_002241"
     CDS             complement(117..602)
                     /locus_tag="ACG8KN_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189254.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(741..956)
                     /locus_tag="ACG8KN_002242"
     CDS             complement(741..956)
                     /locus_tag="ACG8KN_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFP9189255.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1180..1980
                     /locus_tag="ACG8KN_002243"
     CDS             1180..1980
                     /locus_tag="ACG8KN_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9189256.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1999..3867
                     /locus_tag="ACG8KN_002244"
     CDS             1999..3867
                     /locus_tag="ACG8KN_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9189257.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3860..4351
                     /locus_tag="ACG8KN_002245"
     CDS             3860..4351
                     /locus_tag="ACG8KN_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9189258.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4339..4893
                     /locus_tag="ACG8KN_002246"
     CDS             4339..4893
                     /locus_tag="ACG8KN_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP9189259.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4911..5906
                     /locus_tag="ACG8KN_002247"
     CDS             4911..5906
                     /locus_tag="ACG8KN_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP9189260.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(6048..>6816)
                     /locus_tag="ACG8KN_002248"
     CDS             complement(6048..>6816)
                     /locus_tag="ACG8KN_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9189261.1"
                     /translation="LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELP
                     LEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRAL
                     EKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYA
                     GLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPK
                     HQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         2310 a         1104 c         1368 g         2034 t
ORIGIN      
        1 ctgtaacgaa ttattaggtt aatgcaaatg cttatttata ctccgtataa ctttctcaga
       61 aaaaatgata ccaataatga cttgctagta ctgtaaatcc gtataatatc atctaatcaa
      121 aatggtgttc catttgcatc tgtcatcgta atcgatcctt cgggtgacac tcgataaaaa
      181 ccaacgcttc cagatcctcc ttgggaacgg atttgtttgc tggaagctgt gaacaaataa
      241 tctcgatcaa ttttttgata aaagctaata acaatatcgt cactttgtaa ttcggaacgg
      301 tgggcaataa gaaatagaag tgctttgtca gagttttcta atggaagaga aggtacagtg
      361 ctttcttgca tttcagatgt tgaagaaatg ctaaacgact gaatacttga ttggctatgt
      421 tgcgaatcta cattaccagt agaagtatta gtaatggaag tagtagaagt acttttagtt
      481 gaaccaacct cagttgttga aatggctgct gaagattctt taacagaatt ctttgtttta
      541 tatccacagg cagaaaaaag tattacagtt cccaacaagc ctgcgtaaat ccctttatgc
      601 atgatagctc ctcctaaaat agattatctt tattcagtat atcacttaaa caaagaaaag
      661 cagatggttt aaaatgaaaa aaccttccca ataaagggaa ggttcatgaa gataataact
      721 tttttaataa ttatagcttt ctaatatctt tcatcaactt ttctagtaat cattgggaaa
      781 aatttcaagc agtagatcgc acaaatacct acgacgatat aaatgatctt cgcaaaaata
      841 gttgttgaac caccagcaat catagctact aaatcaagtt cgaacaagcc taccaacagc
      901 cagttcaacc ctcccacaat caataaagtt aatgcaatag catctaaagt tttcattaga
      961 attctccttt cttaaatgat aaaacaatta tatatcaaaa agagaaacat tgcttatata
     1021 acgctcagga attttagcaa tctatgcaaa aagatgaaaa ttttttttgc tcttccatta
     1081 gtacacattt tttagctgaa gctgtaaatt tcatgtctta atttttttta gtgagctttt
     1141 atactggatt tgtaagaaaa aagaaggaga ggatcacata tgtcggattg gttggatatt
     1201 gcaggaaaag tagtaattgt aactggaggt tcctcaggaa ttggaagaag tatcgtcgaa
     1261 aatttgttga agcagaatgc acaagtagcg aattttgatg taactgaatg ccgtatacag
     1321 catgaaaacc tactatcttt aaaagtcgat gtgtcttcta aaacggacat agaagaaggg
     1381 atatataaag taatgaaaca tttcaagacg atcgatggtc tagtcaataa tgcaggaata
     1441 aatattcctt ctcttctaat agatagaaat catccaaaaa gcaagtatga attatcagaa
     1501 caagtttttg ataagatgat cgctgtcaat caaaaaagtg tatatttgat gagccaagca
     1561 gttggaagaa tcttggtgca aaagggatca ggagtgatcg tgaatctttc ttctgaatca
     1621 ggattggaag gatcagaagg acagagctgt tatgctgcta caaaagcagc gatgaacagt
     1681 tttactcgtt cttgggcaaa agagttagga aagaggaatg ttcgtgtggt aggtgttgca
     1741 ccagggatac tggaagaaac cggtttgaga acacaagaat atgaagaagc tctttcttat
     1801 acgagaggta taagcgtcga acagctgaga aacggttaca gtcgaacaag tacgatccct
     1861 ctaggaagga gtggtaagct gcaagaagtg gcggatctag tctgctatta tctttcagaa
     1921 agatcaagtt atatcactgg agtcacgact aatatttcag gaggtaaaac aagaggataa
     1981 aggatagggg ggacgcacgt gacgctagta aacagatggt accaattatt gaaattacta
     2041 gtcactcaca agaaagtttc aatccaggaa gtgcaaaaag aaatcaaaac cagtaatcaa
     2101 accataaaaa aagatattgg ggagttgaat gaagaaattg ccggtattgc aaaaatcatc
     2161 caaaaaaaca aacattttga acttgaaatt tatgattttg atttttttga tgagatcatg
     2221 caaggaaaat tgaaaacagc cagtgatttc aattcggcat ccaaaagaat cgcttattta
     2281 ctaaataggc tgatcgaaac agaggatttc ttacgtatgg atgatttgtc tgaagaagtc
     2341 ggtgtcagca gaggaacagt ggtcaaggat ctaaagacat taaaagagat gatcaatgat
     2401 tttgatgtaa agattacagg cacaccaaat aaaggactaa gaataatagg agatgagcta
     2461 gaattacgac tacttttatt ttattttgtt tctccctact tttcagagac gaaaacagaa
     2521 cagtttctag aagaaaaaca agcaatcaaa cattttcaga agaagacgaa tgctagtaaa
     2581 caagaaatca cccttttaac gaaagttatc ggtatttctt tgaatagaat ctctagtaac
     2641 tatctgttga agaagcccat caatcgttac agtgggtgtt ttgaatatat ccaagaattc
     2701 aatgaactga tcaatcagtt ggaagaaatc tatgaactaa ctttgagcca atatgaaaaa
     2761 gattttatca gttttccttt gaacatcatc aacacaagta taacgatgat aaggaaagct
     2821 aactctgatt tgcgcttata tttcgacccc atgatgatgc gtattcgacg attagatctc
     2881 attgatctgg atgagtcctt cttatatcaa gagatgaaac agcacctctc tttattgata
     2941 aatcgggtaa tttttcgtta tcgattgact gatttatttt atggtgagat agaaaaaaag
     3001 tatccttttt cttaccaaat cgctagcgaa tgcatacaag cattagaagc tatcttgcaa
     3061 cataagatct ctaaagtaga gatcagctac ctaacgttgt atttggagtt agcatttcga
     3121 tatcaaaaag agcatttttc ctctaaaaaa gtagccattg ttcatacgac aggaaaagga
     3181 acagcattaa tgatccaaag acaaatcaaa aggatattgg ggagggatat acaaataaca
     3241 catgtttcag aagaacaata tgaaaaagta gatctaaaca aatatttcgc tgtttttaca
     3301 acgattccgt taaaaaatgt aaatgaagag acaccggtga tccatttgac taatcttttt
     3361 gatgactctt ggttaagggc acaatggcaa aaagcaaaaa aaaagcgatt agccgggttt
     3421 cattctactg tttttttcta tcaaatatgg aaagaaaaaa agacgtatca agaatattta
     3481 aacaaattag tcgctccttt gatcagaggc tattatgtgg atgaagaatt tgaaaaaagg
     3541 atcttgaaaa gagaatatca gcaaagcaca tttgttaaca aagaatttgc ttttcctcac
     3601 acaaccaacc agctatctca acacattgtt ttatcagtag ggctaataac tgatcaaggt
     3661 ttagcagaaa caggaggaaa tggagttatt tttttatttg ccatacctga agaaatgacg
     3721 gctgaagttg agaatgaatt atttgagcta tacgatatcc tatttgaact cataaaaatg
     3781 ggaatagatg agtttattga attagcaccg acacaagaat catttttaaa actaatcaaa
     3841 agtaaaggag agagacttca tgaatgatat gtatctattc atagttttcg cagcttcagc
     3901 ttatgtgctg caagcagtta tcagttggaa gcaactaaag catttcaatc aaacatatag
     3961 cgaactgcga agaaaaggaa aagtagcgat tggtaaacga cctgggaaaa tccgttcagg
     4021 cacgattgtt atgctagcta tagacgatca tgatcaagtt ttagaagtca gaaaaatgca
     4081 aggggttaca tctttggcaa aatttcattc attacctgtc tatgcgggtg aagatatcca
     4141 ttacatcgat cgttaccatc cctcagttcg agcggaagat cgtttgacac aagcagcttt
     4201 agaaaatgcc agagaagtat acttacgaat agaggcagga aatgaaaaaa gtgaacaacc
     4261 ttcattgcct tttattgcta tgctcaataa ttttcggata tggaaaagta cgatcaaaga
     4321 aaaaaggagt gacaaacgat ggatatcata acaaaatttg ctgagggatt tatgaggctg
     4381 ttccaaacag gtgcagaaac attcataagc tggatgacag gaatcgtacc agttgtcttg
     4441 atgctgatgg ttgcaatgaa tacgctgatt gcttttttag gtgaagaccg agtaacgaaa
     4501 gtagccaaat tatcagcgaa aaatccgctt actcgttacc ttgtgttacc gttcttatct
     4561 gcttttatgt tgggaaatcc tatgtcattt acaatggctc gtttccttcc agaatactac
     4621 aaaccaagct attatgctgc tcaagctcaa ttttgtcata cgagcaatgg tgtattcccc
     4681 catattaatc ctggagaatt gtttgtatgg atggggattg ctcaagggat cgaaacctta
     4741 gggctaaatt cgatggagct ggctatccga tacttgcttg tgggactgct gatgaacttt
     4801 attggtggtt ggataactga ttttactact ggatttgtat gcagacagca aggaatcgtt
     4861 cttagcaaaa aagtagagct ttcagttgat tagagaaagg agagttttaa atgtatggtg
     4921 atattaagat cgtaagagga aacggcgggt atggaggtcc tttgattatt aggccgacaa
     4981 aagataaaca caagttcatc tatgtcacag gtggaggtga gaggccaatg atcgtagata
     5041 aaatagaaga actgacaggt atggagtcta tcaatgggtt caaaacatca atacctgata
     5101 gtgaaatcgc gcttgcaatc gttgactgtg gaggaactct tagatgtgga atttatccta
     5161 aaaaagggat accaaccatc aatatcgttt ccacaggaaa aagtggccca ttagcacaat
     5221 atatcactga agatatttat gtttcagcag tagggttaga acaaatacag ccactaatgg
     5281 aaactgatga taacaaagaa acagtaaata ctgcatctcc agataaaaag tacaaatatg
     5341 atacttctaa aaagataacc gagcaaaaag cagagcagca aaactggata gcaaaaattg
     5401 gaatgggcgc gggaaaagta gttgctacat tcaatcaatc agcaagagaa gcagtagata
     5461 cgatgatccg gacagtgatc ccttttatgg ctttcgtttc ccttttgatt ggtgtgatac
     5521 aaggttctgg gatcggtaat gtatttgcta acattatggc acctttagca ggaaatgcag
     5581 ttggattaac aatcattggc tttatttgtt cactgccatt tttatctcct ttgcttggtc
     5641 caggggcagt tattagccaa gtgattggaa cattgatcgg tgtagagatt gggaaaggaa
     5701 atattcctcc ccaattagca ttaccggcac tatttgctat caatactcaa aatgcatgtg
     5761 attttattcc agtcggtcta ggtttggaag aagcaaaagc tgagacagta gaagtaggtg
     5821 taccatcagt tctttattca agatttctta acggcatccc acgagttatc attgcttggg
     5881 tagcaagttt tggattatat agctgagttt agtctaatgg tgataagtca agatgaaatt
     5941 taagattttc tttattgagt aatatgtttt gaagcgcacg aaactagacc cgatcaaaat
     6001 caaatttttt tcgggttgat tcaaggattt gcgtcaataa cggattgtta tttctctatc
     6061 acactccttg gtggcgtata gagttggtgt ttacgtagta gcgtatccac cagacgcgta
     6121 aattttcttg cggttaggac gagtgcacgt ttgtgttgat gctttggaac ctccttatat
     6181 ttactttgat aaaaagcttt gtattcagga agataatttt ttacagagtt ggcagcttca
     6241 actaagtagt atctaaaata tcgatttcct tgttttgtca gaggtgtatt ttgcgactga
     6301 tagtttccag attggttcac tttccaactt aatccagcgt attttgccaa ttttgtctga
     6361 tcttcaaatc tttcgatttg accaatttca gcgattaaac cggcagcata gactttacca
     6421 acgcctggaa tactggttaa gcattgatat tctggaatga caacaattac ttgttcgatg
     6481 gctttatcta attctttgat atttttttca agtgctcgta tttctcgtac gagaacactt
     6541 aaaacaacat taattgattc ttgtgcaaga gcacctaggc ggtaactcat ggtaattgca
     6601 cgttgaattg ctttagcgat tttttctggt tgcttgaaac ggccttttcc tttttcttga
     6661 agtaaatcac agaaagcttc taaaggaagc tcagcaagtt catccaatgt gaaatcttcc
     6721 gtcatgagtg aaatgagggt tgcactaaaa agactcgtcg attcgtctcg catttctcga
     6781 gcaagcgtat tacatttgta cgttagattt tcaaga
//
LOCUS       DAZKCZ010000109         6318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000109 DAZKCZ010000000
VERSION     DAZKCZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.112"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(789..1130)
                     /locus_tag="ACG8KN_002249"
     CDS             complement(789..1130)
                     /locus_tag="ACG8KN_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9189262.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1130..1360)
                     /locus_tag="ACG8KN_002250"
     CDS             complement(1130..1360)
                     /locus_tag="ACG8KN_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9189263.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1647..1985
                     /locus_tag="ACG8KN_002251"
     CDS             1647..1985
                     /locus_tag="ACG8KN_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189264.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2190..2752)
                     /locus_tag="ACG8KN_002252"
                     /pseudo
     CDS             complement(2190..2752)
                     /locus_tag="ACG8KN_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3484..4065)
                     /locus_tag="ACG8KN_002253"
     CDS             complement(3484..4065)
                     /locus_tag="ACG8KN_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9189265.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4248..4874)
                     /locus_tag="ACG8KN_002254"
     CDS             complement(4248..4874)
                     /locus_tag="ACG8KN_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9189266.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4896..6227)
                     /locus_tag="ACG8KN_002255"
     CDS             complement(4896..6227)
                     /locus_tag="ACG8KN_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFP9189267.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
BASE COUNT         1858 a         1280 c         1005 g         2175 t
ORIGIN      
        1 aaaagtgact caaaataaaa aaaaccttac attatactgg aatgaactgt attgatagtg
       61 tacctaaaag ctctactgac ataagatcgt gtaatcggta aagtaatttt attaattata
      121 cgggatgtaa tcaattagag aataaactaa tcataaaagg tacttttaca ttcataattg
      181 tcgctttgag attctacttt ggttcactat ctgacccaat tgcacatttt cgatcattac
      241 aagtattggt gtcgttagaa caactaaata aaaactagcg gtagattttt cagaatctat
      301 gctaatttct gtatggatgt tccagtgatg gcaaatttct cttttcattg tttttcttgt
      361 cgcatgatat gcctttttgt gttattcagt taaactatag aaattctttc ctagacagat
      421 cttgatccca aattttacgt ttttgagcat cgatatagtt taaaatgaca ttactcatat
      481 ctaaagagtt tagatttttt aggactaaaa ctgtcagttg ttctttttct tctttctgtt
      541 aaagtattgt cgcgactttt cgtatacgtt aagatatgtc gtgaaaaaac aagttttgtg
      601 acacaaaaaa acgaatgact tgaggaaatc acctcaatca ttcgtttaaa atgctatata
      661 ttctttagtt taagatacgg aaattaatgc catcgaccac actagtagaa tgaacgacat
      721 ttacttggcc tttttttaat ttcgttatgt ttagcatata aaaatattat agagtttact
      781 tacaatgctt aaaacatata ctgtataatc tgatcaattt tagccatgtc ttgcaatggt
      841 aagttttcta tttttttgag ttttcgttcc ttaaaatcaa gagattttaa ttgtgtaata
      901 attacttgtc cttttatatc tagctcttca ggtaatgagt aacgtgtcgg taaattcttt
      961 atagtggaag taattggaca tatgaccgaa aacattgttt tacggttaaa ttcataacga
     1021 gagacgacta gtcctggtcg tcttttttga atttcttttc ctacggaggg atcaaaatca
     1081 atccatacaa tatcaccttt tttaggaata taaattcttt cttctgacac tataaaattt
     1141 ctctcccttc aggagttaaa tcttcccatt cgtctttctc ataatactcg gcctcttccc
     1201 caccactaaa tggatcttca atttttggaa ctaacgtaat tgttccatca tcagaataca
     1261 caacgatata ttcttggttt tctgaaggtt tctttccgtt atcagaaggt aaagttacca
     1321 ctacagaact tccttgtaat ctagattttg taacaagcat tatcatcact cccttaataa
     1381 aagtatacta tataacacta agtaacgcaa aattcccctt tgtaaactat ctagaaaatt
     1441 gttttgttta aagtatatct tattggatat aaatagaaat aaattgtagc cagttcattt
     1501 tttctttaac taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca
     1561 aatactatag ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa
     1621 tttcaaagac aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct
     1681 tttgctctga caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa
     1741 ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta
     1801 gacaagactt tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt
     1861 tacatgatcc aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag
     1921 aatatacaac tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt
     1981 cataatgata aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa
     2041 atagaagcta tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta
     2101 tgagcatttc ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt
     2161 gccgctcttt ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc
     2221 tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct
     2281 tcagtcaaga caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta
     2341 gtcattatga ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata
     2401 tacactattt tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcattg
     2461 ggaaaacaca acgattttgc aaaaatggtt gatgaaaggt ctttttttct tctactgtct
     2521 ctgcacgggc attaatcatc aattgtcctt ttttaaatcc ggtaaatccc cacttcatga
     2581 tttccggagt gaggatgttt tcttcctctt ttcttaacgt cacaacaaga tttgacggaa
     2641 aaacttctcc agctgctaat tctgctgcct gcttcggttg tttcgtctgg gcttctcgat
     2701 aataattctg aaggagctct gactctagat caaaaaaata acgaccacac atcctaatcc
     2761 ctcccaatct ttttttctat catacactgc aacaagaagt tcgcaccaac aaaacgcttt
     2821 caatttttgt agaactaaaa aagttgtgac aagtgacaaa ctctccagtg aatggtattc
     2881 tcctgccaac tcctcggtca caagtcacaa tattcaacaa acatgagaag ctgctttttg
     2941 aaattgttcc ttgtgactcg gagtgaaacg gcaggcttac gacctctggg taaacggtgt
     3001 tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtagaa agctattttc
     3061 acaaaatttt tcttaatact cgaaactaag cgactttttc acaacctctt ccttaaacgg
     3121 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gagcagctat
     3181 ttttatttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tctccactaa
     3241 acggtgttca atcagctaac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
     3301 attttgccgt ttgtttctct aatactcagg tcaaagcgct gatttcacaa cctctaatac
     3361 acggtgttcc agaagtagct tcttcgaaaa ataagccgaa atttcacaaa aatttgagaa
     3421 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctctaa
     3481 ttattaaaga accttttgcg aagttaacaa tctttctttg atcaattgaa aattctgcct
     3541 aagtagtgat agatcctgtg tgactttcaa caataagata cttccctgga tttcagcaat
     3601 caacgctttg gcttgacggt ctgcttcttc atcggacagt ttttcttcag aaagtgccag
     3661 gatattttgt ttcgtatact tgacccattg attgactaat tctagtagca acagacggta
     3721 ttcttcatcc actgtactca actcaataat caaattacct acaggacagc catgataagt
     3781 gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt tcttctaatg cttcttctgg
     3841 ctgttttcca gcatttaaga tgtcttcgaa caattctgtc tgccatacgt ccatatgcgc
     3901 atggatcacc gctagacaaa cttctttttt tgaagaaaag taataataaa attgaccttt
     3961 tcctgtatca gaagctgcta gaatatctcg gattgacgtt tcatgatagc cttttttata
     4021 aataagatct ttggtcgctt ccaagatctt ttcctttgtt ttcaatgata ttctcctttt
     4081 tcatttctca tagtcctata ttatacagta aattcatcat tttttcttct taatcgttta
     4141 aaaaagactg tgccaaatta tctgacacag ccctcactat aaaactgttc tcatatagct
     4201 agatgttcat tgattcaaga actgcgagga caaaagcaat tcctatctta tgatagttgc
     4261 tctaatactt cctctgttgt catcacttga tctgcaataa agccaaagtt cgtcaacgct
     4321 gcatccattg cttcttggcc aggagcacca attgcatctg tcgctaccac aacattgaat
     4381 ccactctcta ccaattcacg catatgggaa tcgctacaca agtttccgtt cattccacca
     4441 agaattactg tgtcgattcc attttttctc aattggagcg ccaaatcatt gctttctggt
     4501 ccaaagattt tatgaggaga gctgatgatc gtattttcat ccaaataagg tttcaattct
     4561 tcgataaaat cggctcctga accttcttcc actgattcat attgactttt tctagcaaac
     4621 atctgctgct caagcatcat atgttctcct gcaccgccaa attgccattt atggtcatgt
     4681 gggtaaaaat aatgaggaga aataaagagt ttgtatcctt tttcttttgc ttgttttacc
     4741 atttcaatca tattttcgat cgtattgtat ttttctaaga tatcttttgt caaaccaaag
     4801 ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag tgagaactac tgctgtattt
     4861 ttattattga acatggtgga ttctccattc gttatttatt ttaatacatc atttagagct
     4921 tcactcattg tcggatgaga atagatttga tctctcaata acgtataagg aagttttcca
     4981 ttcatagcta gcgaaatcaa gttgatgact tcatgagaat cttccgcata gatacttgcc
     5041 cctagaatca agtcagtttc agggtcgact aaaactttga atacaccttt aggatcttct
     5101 aatacttgtg ctttagggac accgcttgtc atatacttga ataatttgta attgacgcct
     5161 tgtgctttgg cttgtttttc tgttaaacct acattggata aagtcggagt gataaataca
     5221 ctgtaaggca cagctttccg gttattcgtt gttcgtttat tctcgccctt caactgatcc
     5281 ataatgattc gataatcatc tagagagata taagtgaact gcaatccgcc tttgacgtct
     5341 ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt catccacagc gattgctccg
     5401 cgatctgtta gtttaacatc tgtattttct aatccaagtg atgctgtact tggttttctt
     5461 ccagttgcag ccaaaacttt tgacgcttgg attgttactt cttggccatc ttctgtaaag
     5521 gtgacggcag ctgttgattc ttggtctgct acttgatcta cagatacacc tagatggaat
     5581 tcgattcccg catttgtcat gtcttctaaa atcatttgcg cgatatcttc atcttcacgt
     5641 ggaagaaatt cagaatgggc atcaagaacg acaactttag atccgtattc attgaacatt
     5701 gatgcaaatt ccaagccaat atatccagcc ccgatgatca ctaatttttc cggtaattgt
     5761 tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac ttgtattcaa tcctttgatt
     5821 ggtaaaatga ttggttcaga accagtattg atgaaaatcc gttcgccttc aacaatcact
     5881 ttttcaccat tattcgtcac ttcgatttga tggttagaaa gaaaacgtgc tgtaccgtcc
     5941 atcactgtcc cagttgcctc atcgctgatc atatgataat ttttggcgcg aagcatgccc
     6001 gtcaattttt ctttccgagc aacagcctct gtaaagtcga ttcctctttc tccattgaaa
     6061 atcaatgatt tagaaggaat acagccgatg ttgatacatg ttccgccata catacgtgtt
     6121 gacttttcaa cgactaaaac cgattcacct tttgaagcta acgccttcgc taaagtctta
     6181 ccgccctttc caaaaccgat cacaatattt tgatattttt ccatcatttt tccctcgctt
     6241 cttttttacc taccgattgg tacaaaaata tgataaccga taattgtcta ttctccaaat
     6301 catctgcttt atttgtca
//
LOCUS       DAZKCZ010000110         6311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000110 DAZKCZ010000000
VERSION     DAZKCZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.113"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8KN_002256"
     CDS             complement(99..1229)
                     /locus_tag="ACG8KN_002256"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9189268.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8KN_002257"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8KN_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9189269.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8KN_002258"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8KN_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9189270.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG8KN_002259"
     CDS             complement(3485..4552)
                     /locus_tag="ACG8KN_002259"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9189271.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8KN_002260"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8KN_002260"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189272.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8KN_002261"
     CDS             4880..5692
                     /locus_tag="ACG8KN_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189273.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8KN_002262"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8KN_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189274.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1875 a         1297 c          996 g         2143 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt t
//
LOCUS       DAZKCZ010000111         5938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000111 DAZKCZ010000000
VERSION     DAZKCZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.114"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..337)
                     /locus_tag="ACG8KN_002263"
     CDS             complement(35..337)
                     /locus_tag="ACG8KN_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9189275.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(557..923)
                     /gene="ssrA"
                     /locus_tag="ACG8KN_002264"
     tmRNA           complement(557..923)
                     /gene="ssrA"
                     /locus_tag="ACG8KN_002264"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1094..1867)
                     /locus_tag="ACG8KN_002265"
     CDS             complement(1094..1867)
                     /locus_tag="ACG8KN_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9189276.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2011..2361
                     /locus_tag="ACG8KN_002266"
     CDS             2011..2361
                     /locus_tag="ACG8KN_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP9189277.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2342..3058
                     /locus_tag="ACG8KN_002267"
     CDS             2342..3058
                     /locus_tag="ACG8KN_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFP9189278.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3058..4506
                     /locus_tag="ACG8KN_002268"
     CDS             3058..4506
                     /locus_tag="ACG8KN_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9189279.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4576..5085)
                     /locus_tag="ACG8KN_002269"
     CDS             complement(4576..5085)
                     /locus_tag="ACG8KN_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189280.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5075..5800)
                     /locus_tag="ACG8KN_002270"
     CDS             complement(5075..5800)
                     /locus_tag="ACG8KN_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189281.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2017 a         1039 c         1154 g         1728 t
ORIGIN      
        1 tccgttccgg gctacctttt ttaggtagcc tactttaatc tttatacctt tctggatcaa
       61 cgaaagtata cttcaaatag tcataacgcc gatgatcgct ccgtgcgtct ggcacatcag
      121 tcacgatatc aaacaaaaaa tacacatctt tcttcattct ggtttttgtt gccggaattt
      181 taaagtagtt cttattagag taatagagat tgattaataa gctatcttcg attgctaaaa
      241 agaaaacttc tgaatcccac accttataaa aatctttgac aaatctattc gaaggatcaa
      301 atttaaacca taattgtgtt tttccttcca tcaacataat gttcacctca aaaagagtat
      361 acgaactaat gttctttttg taaagtagga atatcattga taatatggat ggaacatata
      421 tattaaaaag tgttcttttt gaatgctatt ttgaatgcca ttttcattca tttaacgaaa
      481 atctgcgaaa ataaaaaatg ctcaaaccct tataaaataa ggatttaagc atctagcgaa
      541 atctgctgaa atctcacaat ggagacggcg ggagtcgaac ccgcgtccaa acacatcgcc
      601 acttaaaaat ctacgttcat agtcaactca tttaaggatt cactttacag cttgccgagt
      661 gacaggcatt ctgtatcgct agtctgatgt tctctttttc aacttacaga cggaaagttg
      721 aaacgtatcc cactaaagtt aggaccctta ctcgagcaca tgggcgatgc cgagaggatc
      781 tacgctaact gtttttaggc agctaaagcg taagagttgt tttcgttttt agcagttata
      841 tttaactgta acgtttttac gtagacgtaa cctacgaaac gcaattcaag ctcgactgtg
      901 cctgtcgaat ccgtaacgtc cccgttagct aacaagggtt acgtgtattt ttcatacaaa
      961 attagtatag catatattgg gataaattca ataaaaaagc gaatcatatc ccattattta
     1021 tataataact gcccctttct taagaaaaaa taagcgattt tcttaagaaa agaggtagct
     1081 tcaaaaaaag atcttaaatc agatcaaaat acttcaatgc gtggacaatt ccgccttctg
     1141 tatttttctt tgtgataaag gtagaaagtt cttttaatcc gtcataggcg ttgcccatag
     1201 cgattttgtt gtcgcaagct tccaacaagg caaaatcatt cggaccatcg ccaaatccaa
     1261 aagtaggaac tttttccagc tgaagttctt tttgcagacg tttgacacct gccccttttg
     1321 agattccttt cttcactgta tcgatcgagt aaggaccatt ccgataaaaa gttagctcag
     1381 gaaaatgttc gtgatagtat tcgtcacctg attgagataa gatcagcaac atatttactg
     1441 tgttttcttt aaagcctaaa ggatcaattt ccggaacatc tgaatgaatg aagtcgtaag
     1501 cattgatcat tgtttggcta tgaccggtac accagatttt ctgatcgtta tagtaagaaa
     1561 gttcatgacc gttcaactgt acatgatcat acattttttg acattcttct gaagaaaatg
     1621 tttgagaata gatttcttcg ccgtctactc gaatgaaaga accattcatg gtgaccgcag
     1681 aagtaatgcc cgcatcttcc atgatagagt ggatttctgc ttctgtacgt cctgtagcga
     1741 tgacggggag aacattgttt tcttttaatg cgttcatagc ttttgccact tcgggtgtga
     1801 tttttgattt cgcatctagt aatgttccat caagatcaaa gaaagtgatt gcacgataat
     1861 ttgacacaaa taaaactcct tttttattgg aataagttta tcttaacaaa gaatgaacaa
     1921 aagacaaacc tttcgtgaag aattcctgaa catattggtt cagtcaacaa tttagagtat
     1981 actataagaa gagaatttag gagggaaaga atgatttttt tcagtattgg tatcttattc
     2041 ttaataattg gcagtctttt tcggattctg ccctcaaaag gaaatctgcc tttttatggg
     2101 tatcattcgc ctcttgccgc aaaaacagac gctcattggc ggctggctca aaagacaagc
     2161 ggaaactggt tttttcttat gggcttactg atggcattga tcggctatta tttaaagaca
     2221 tctgggcata cgaattattt tctgattgaa atgcttctgc tagtatttcc aattatgcct
     2281 atttttatta tgacagaaaa gaaattacag aaatatgacc tggaaactgg agggaacgat
     2341 aatgaatatt ttaatgattg aagataatca atctgtttct gaaatgatgc agatgttttt
     2401 cttaaatgaa ggttgggaag caactttcaa gtatgatgga aaagaagggc tcgatgcctt
     2461 tttagaaaac ccacaacatt gggatatgat tacactagac ctgaatttgc ccacactgga
     2521 cggtatgcta gtaggaagag agatccgcaa agtttcaagt actgtgccga ttatcatgct
     2581 gacagctaga gattctgaaa gcgatcaggt gatcggctta gagatggggg cagatgatta
     2641 tgtcacaaaa cctttcagtc ctctgacgct tattgctcga atgaaagctt tgcatcgccg
     2701 tagtgagttg gcagaagcaa gagaagaagc agtagtttca gatgatcatt ttgatatcga
     2761 gactagtcat ttcaagatga atacgaaaac acgagaagcg tatctggaca accggccaat
     2821 tgaaggactg acgcctaaag agttcgatct attgtttact ttagcaaaaa aaccacgtca
     2881 agtattctct cgggagcagt tgcttgaatt agtctgggat tatcagtact ttggtgatga
     2941 acggacagtg gacgcccata tcaagaaatt acgacaaaaa atcgaaaagg tcggaccaca
     3001 agtcatccaa actgtttggg gagtcggcta caaattcgat gattctggtg ttgcctaatg
     3061 cgttatttgt atcagcaact cttagctttc tgcggaatgg tcgcgttgat cttattgatc
     3121 gttggagttt cttttacaca actgacaaaa caaacgattg aagaaaacaa ttaccagcaa
     3181 ctgtttggat atgctgaatc agttgaaaag acaacgcaaa cctatgccga tacgctcccg
     3241 cagtttagtc aagatcaggc gtttcaaaat gcattgctgc tgacagagca agtactgaca
     3301 gaacaaaatg tgaactttat atttatcgat aaaaacgaac gtgtcattta tccgactaat
     3361 acagctttac tgaattttac cttaacagct gatcaatgga aaaatctgag gaatggacgt
     3421 caagaaaaat tcacatcgaa taaaaatatt ttaggacaag cacaggcgac ctcttacgca
     3481 cttgtgccat ttaatttgaa tcatgaattc tatggagcac ttgtagttag tcaacctgcg
     3541 cgaaatatcg ataacagcgt tcgtccaatt acactgaacc tgttcaaagg atttattttt
     3601 tccagtatcg ttgcagttat tgccagctat gcttttgcag cattccaagt taagcgtatc
     3661 aatcggttaa ggaatgcgac gaaagaagtg acgaatggta attttgatgt acagctcccc
     3721 gtacatgaca aagatgaatt tgatgagtta gcagatgatt tcaataaaat gacgaattca
     3781 ttgaaagaat ctcaagcaga aattgaagaa caagaaaatc gtcgaagaca atttatggca
     3841 gatgcttctc acgagatgag aacacctctt acgacaatca acggacttct ggaagggtta
     3901 gaatacaatg caatccctga aaatcagcga gaaaacgcga ttaaattgat gaaaaatgaa
     3961 acagagcgtc tgatccgttt ggtgaatgaa aacttggatt atgaaaaaat ccgtacgaat
     4021 caaatcagta tggtcattaa aaaattcaat ggaacagaaa cattgaaaaa tattgttgca
     4081 cagttagaag ctaaagcaga agcagcagga gatacattga cattgaaagc cgttgatgat
     4141 attgatgttt acgctgatta tgaccgattt gtccaaatca tggtcaatat tattcaaaac
     4201 gcgatacagt ttactgaaaa cggccaaatc acgattactt tagaaaaagg ctatcttgaa
     4261 acgatcatca cgattgaaga tacgggaatt ggaatgtctg aacaacaaat gaaaagtatc
     4321 tgggatcgtt attataaagt agatccttca agaaaaaata caaaatatgg tgaatcagga
     4381 ttaggtttac caatcgtgca gcaacttgta cgcttgcata aaggaaaatt agaagtagaa
     4441 agcgaactag gaaaaggaac aaaattcgct gttcgtttgc ctgacgtaga aatcaaaaaa
     4501 gagtaaaaaa caagcggtat aaaagatcat gaatcgatct tctataccgc ttgtttttac
     4561 tataaaatga ttgatttagc ccgcgggctc taaacgatca atataaggac tttgagcaat
     4621 agtagcatca tgagtaaaga gtaagatacc ttgattttct tcatgagcta aataagaaag
     4681 caagtggagt agctcttgga tctccaatac actaagttca tcaaatgtat cttcaataac
     4741 aatattgttt cgcttggaaa gcaaggcatg aagtaattgc agctttactt tttcaaaaga
     4801 cgtcaactca gctgccgcac aattaagcaa ggaaggagag atatcaagtt tttcttgata
     4861 ggccaaacga tcttgtttga tttctttttc cggtacatcc aacaggacat ggtctttaag
     4921 attcagataa ggaaacatcg gccaattttt ttggatgatc gcaaaattat ccagttttgc
     4981 aacaagaacc tcttttttca agtgctccaa tattttatgc tgtttttcga gaggacacaa
     5041 aataacggtc aagtgttcag catgaatcaa atattcaggt ttcattacgt ccaggcctcc
     5101 tactttgcaa atgatggata aaaaaagtga aagggaatag actaagaatg agacagcatc
     5161 cactgagccc aataagagtt tgggcaaatc ccttaagtat atctgtgaaa aaaagatcat
     5221 tttggaaatc gatggtaaaa aaagtctgtt gatccatcgg aattgtaagt ccgcttaccg
     5281 aagtcaagat atcacggata tctggcagtt cagtagtctt taagacaaga aaattgatcc
     5341 tttgaagaag cgcttcataa actgactgga agcttacgat ccacaataaa accaagatac
     5401 aacaaagaaa gagaggaaag aacagctgcc agaaaataaa aaggaataat ttggttttag
     5461 aaagaccagt caggtgccaa ttgatcaatt cctgcttgtt ttgcctgact gaatagtaaa
     5521 agaaaaagat aaaaaggaag gcaaatagaa aaaataaact gaatatgcct tttatataaa
     5581 atcggctagc taattctaaa gccggaagaa gctctgtaga tgcagaagcg ccaataaatg
     5641 aaagccgatc ttggacttga ttggctaaag ccgattgcaa atggtaaaga gtcaggcatg
     5701 ctatcaataa aaaagcagaa aagcttgaaa ataaacaata gagtattgtc tgctttttgt
     5761 aataaattaa tcgagcagta gcatagcgga ttattttcat acttttcacc tctataaaaa
     5821 gtatagaaaa tgaatatgaa taaagtatga aattccttta gtgaaatcac gaattaaaac
     5881 cagaaaatat tttgtaaaaa gcaagaggtt gtgaagaagc tgccctattc tattaccg
//
LOCUS       DAZKCZ010000112         5906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000112 DAZKCZ010000000
VERSION     DAZKCZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.115"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(367..1485)
                     /locus_tag="ACG8KN_002271"
     CDS             complement(367..1485)
                     /locus_tag="ACG8KN_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189282.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1718..1840
                     /locus_tag="ACG8KN_002272"
     CDS             1718..1840
                     /locus_tag="ACG8KN_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189283.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1956..3263
                     /locus_tag="ACG8KN_002273"
     CDS             1956..3263
                     /locus_tag="ACG8KN_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFP9189284.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3382..4581
                     /locus_tag="ACG8KN_002274"
     CDS             3382..4581
                     /locus_tag="ACG8KN_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9189285.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4608..5876)
                     /locus_tag="ACG8KN_002275"
     CDS             complement(4608..5876)
                     /locus_tag="ACG8KN_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFP9189286.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         1813 a         1251 c         1058 g         1784 t
ORIGIN      
        1 ttttaaacgg tgttccagaa gtagcttctt cggaaataag ccgaaatttc acaaaaattt
       61 gagaaataat ttttgtcaat ctcttcttat ttcttgaagc tgaccacttc tgtcacaacc
      121 tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagcccacc aaacaaaaaa
      181 tatgaagagc tatttttgct tgctgttctt attacttgat gcagaacagc tttttcacaa
      241 cctctcatac acggtgttta atcagctgac cttcggtatt agctcaaaaa acggaaaaat
      301 atgtgaaact atttttccgt ttgtttctct aatactcagg tcaaaacact gatttcacaa
      361 cctcttttaa taacgaagaa tcgctgtcag actcttttca tctggcgcat ctttttgatc
      421 caaaataaac actttcccgc cattaatgat cacatcatct gctaatgcat tcaacacttg
      481 acgccgatca tattctgttt caggatcttc accgaaacca tcagctaagt ttgacgtagc
      541 gataaataaa tgtgacactt ttccttcttt cgcagctgga acgatatcaa ctaactgatc
      601 gatgaatttc ttatctaaga gtttattgta ttcttttgat tcttgagctg cgagttcact
      661 tgacagcttc ttcgcccctt cttcgatatc tctaaaggat agctgcgcag gagaagcaga
      721 aatcgcaacg tctgcacaat aatagggatt tttagcaatt tttttgaaca tcgtctgatt
      781 ttctggtaat gcaaataaat aaatcggcca ttgttcagga ttttcgaaag aatccttcaa
      841 gtacgtatca acagcttggt aatagttgac ccaatcgatc tctacttctt catcttttgg
      901 atttacgcca tggaaagtta ctccatctcg ggctccgcct cctccattag aagagaagtt
      961 caaaccgcca cctgtcagtt cagatcccaa tgcagtaacg acatcaaccg gtgccccttc
     1021 tggtagagat acttctttta cttgtttgtt gctgacctca tacaatttca tggagtcgcg
     1081 gttcaatccc aataaataat aagaatagtt gaactgccca tttttgataa tagccagtaa
     1141 ataaggaaga tcacttacat agtattgatc gtctaccggg atactcaaac gatggatata
     1201 ggtctggtca gcagttataa tgaccgctac actcgtagtt gcactacgcc aaaacgaagg
     1261 atctcctaac aaattttcaa ttttttcttg gaacggggac cagtcatgtt cggaatattt
     1321 tttctcaaaa cgtttctttg cttctttcgc aaaatttttg aatttgatct gatctttttc
     1381 cacatcttga tgggcgacat gcgtattcag catgatcgtg acgaatggtc ctttagcgcc
     1441 ttctgaagtc aattgtgtca ataattcttt atcctttttt gccataaacg tacccctcct
     1501 aaaaaaatca ttcctttgtt tctatctaaa gtctaccaca acactatttt tccatacaat
     1561 aaaacgtatc taaaaaaata aaacggtttc tacggtttta aatttagcta tttttttaag
     1621 ttatcttcat aatttcctca attatttagc cccagtcttc aactattctt catgaaatgc
     1681 tgataaacta agaaaaagaa taaaagaggt ggatcttatg catttgatta tgattttcag
     1741 tttattaagt attatcagtt tatttgcttt cagcgcttcc cgcttattag atgcggaaac
     1801 acagcctatt cctgtaaaag tacgtaaaaa cgaccgctaa acttccataa cgcgcgttct
     1861 tatcctaaag ttttttcaga aacattgaaa ctctctcccc tctctgataa aattttaatt
     1921 tgtagcgaaa caaattaatt acaaggagat cggatatgtc cataaaaaaa acacgtctag
     1981 tcctattatt ggctagtctc attctttgcc ttgcttcttt tctaccagct gttgctgtgc
     2041 aagctgaaga tacgttcaaa gtaaatgcaa aagcagcttt tgctgtagat gcgcaaagcg
     2101 gtaagattct ttatgatcaa gatggagaaa aaaccatggg gatcgcttca atcacaaaaa
     2161 tcatcggtct ttacatcgtc cttgatcaag taaaagaagg aaaactgtca tggggtgata
     2221 aagtttcgat ttcagattat gcagaaaatt tgagtatcac cccagattta tcgaatgttc
     2281 ctttgcataa agaaaacacc tacactgtca aagaattatt cgactctgca atcatccagt
     2341 cagccaatgc ttcaatggtg gccttggcag aaaaaatctc tggcagcgaa gctaaattca
     2401 ctgaacgaat gaaagaacaa ttgaaagatt ggggaatcaa agatgcaaca atcgttaatg
     2461 catcaggatt gaacaactct tatcttggcg aaaaccgacc agaaggcact ggagaaaacg
     2521 acgaaaatca aatgtctgct caagatgtgg ccatcgttgc ccgccatttg atcttggatt
     2581 tcccagaaat actggatgta tcaagtacta cgacccaaat gtttggcgaa aatacgcaat
     2641 caccagtgga aatggtcaac tggaactgga tgctcccagg attcgttaat tacaaggaag
     2701 gcgtggacgg attaaaaact ggtacgactg agcttgcagg tgcttgtttc gttggtacga
     2761 tcactaaaaa cgatcaacgg atcatcactg tcgtattgaa tgcggacggc catgcggaaa
     2821 acccgagtgc ccgcttcaat gaaacagacc gtttgatgga ctactgctat gacaattggt
     2881 cagaaaaaga gctcggaaaa gctaacgcaa gtattcctag tctaaaaacc attgatgtaa
     2941 aagacggaaa agaatcaaca gttcctgttg tgcttaaaag ccctgtaaaa gtatgggtac
     3001 gaaacgatat ggatactggc aaattaacga tcacgccaac acttgacaag aagcaagtca
     3061 ctgacaatga gatccaagca cctatggata aaggtacaaa aatcggtacc gctacgatca
     3121 ccttggcaga cgataaatta ggctatttag aagacaacaa tgcaccatca acagagatca
     3181 ttacggataa aacagtggaa aaagcgaata tattcgtgat tggctggcga cgcgtcgcag
     3241 aatttttcgg caacttgttt taattgaaca ataagaaaaa catctttcca tcggaaagat
     3301 gtttttttgc tttttctagt ttaaataaca gcttttttca tctattttcg ttatactgta
     3361 taagaaaaag gagttgaatt tatgaatcta accagagatc tgatcttttt agttctctat
     3421 cttattgggg tattctctat cgtttattgg cgaaaacaga agaaggatcc ttccttgttc
     3481 ttttacggca gtacgtggac atttacggta atcttaactt attttgtcgt gttggaaatt
     3541 ttttattcga ccttttattt tttgattcca ttgttgttct ttagtatctt tgctttttct
     3601 tatttcacag aaaaacgaag gctactgaat ggtcttttat tcaatgtttt actcatcagt
     3661 ttcggcatct acttgtttgt cttgctgtat gaaacgcaaa atatttttct tggcggactg
     3721 atcgccttga tcacgattcc attgctactt gtccttttat ttggtattta tggattgatt
     3781 gtttttcttt tttggaacgg ggtcacagtt ttacgtagag aatcgcattc attagctaat
     3841 cttttaacgt tgatactagc catttttctt acactgtttc ttgtttttga ctttttcttg
     3901 ttaaaatatt tgccgcaatg gataaatgct ttgtttttct gcgttcccct gatcttgatc
     3961 tatctattta tcgtattcta caactttttg actgtctcat tcttgtatca gttcaaccgg
     4021 cctcgataca atcaagactt catcgttgtg ttaggcgctg gattgatcaa tggagaaaca
     4081 gtttctcctc ttctagccaa gcgaatcaat aaggcaattg ctttttatcg ggctcaaagc
     4141 cgtgctacat taaatccgcc catcttattg atgtctggcg ggcaaggagc agacgaaaaa
     4201 gtaccagagg ccattgcgat gaaacaatat gccatggaac aaggaatccc tgaaagagac
     4261 attcttgtcg aaaccaattc gacaaccact ttggaaaaca tgctctattc aaaggaaatc
     4321 atggatcagc aaatgaaagg gccataccgc gcgatcttta gttccaacaa ttaccacatt
     4381 tttagagccg gtctttatgc gcggcaagcc aaattgaaag caaatggtat tggtgcaaaa
     4441 acagctttct actacttgcc taatgcgttt ttaagagaat acctcgccat cttgatgatg
     4501 aacaaaaaaa gacatatgat catttgcgga ttgcttttcg caggctccgc attcttatcg
     4561 ctgattactt tacttttcta aaaaaacaag gtgtgacaaa acatatgtca caccttgttt
     4621 ttttctttca ttctcttaac tattggcaaa aagagtatcc cttttttgac cgctacttgc
     4681 tgagcagaat aaataccgct gttgcctgaa cacataaagc tgatcaagca gatcataaag
     4741 aagtatactg ccgcttgtga accgaacaat tcgatcccca tcacaaagca ggcaatcggt
     4801 gtatttgtcg caccagaaaa tactccgata aagcccaatc ctgcaagaaa aggaacagaa
     4861 acatggagca aaggcgctag tgctgcacca agcgttgcac caatttcaaa caatggtgtc
     4921 acttctcccc cttgatatcc cgcgcctaaa gacaaaactg tgaagaataa cttcccaata
     4981 aaatcataga catgtgcctg tccattaaat gattcttgaa gtaacggaag actcaatccg
     5041 agataacgtt gtgtttgcaa aatcaatgct gctgccacaa ctacagctcc acctatcgta
     5101 tttcgacaaa caggatgagg aatccattgt gcatatcgcg cttttaaaaa gacgatcgaa
     5161 cggctgaata cccaaccaat caaaccaaaa gcaatcccag caacaaaaac tttgaagaac
     5221 agcaacatac tccattcagg aatcacaccc atttggtaat gtagatgaga aacaccatag
     5281 ctttctgtca tgaaatttgc aaacagtgca gcaaagaagc tagggaagat cgcttctgtt
     5341 cggactttcc cgattgccaa cacttctaat ccaaataatg ttccagctaa aggtgtgcca
     5401 aatacagaac taaaacccgc actgattcca ctgataatca agatttcccg ttctgattta
     5461 tccaatcgga acaaacgtgc cgtattatct gctaacgctc cccccatttg gactgctgtt
     5521 ccttcacggc caacagatcc cccaaataaa tgggtagtta tggttccaaa cagtgtaagt
     5581 ggaataagcc gcaacgggat tttttcttct ccaccatttc cttgatcgat cactaagttg
     5641 tttccacgac tagaaagtcc gccgtgatac gcatagagat aagcaaaaag cgcaccacta
     5701 actggtagta gacaaaggag ccaaggatgt gatagtcgga tatctgtcac aactgtcagg
     5761 ctatttaaga aaaaagcaga caagctcccc atgaatatac cgagaatcgc tgtgatcacc
     5821 agccacttca gcatatacac taaaatgcgt actggactcg gtaattgttt ttccataaaa
     5881 aattcctcct caataaaaaa acgcct
//
LOCUS       DAZKCZ010000113         5874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000113 DAZKCZ010000000
VERSION     DAZKCZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.116"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            641..1375
                     /locus_tag="ACG8KN_002276"
     CDS             641..1375
                     /locus_tag="ACG8KN_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9189287.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="ACG8KN_002277"
     CDS             1391..2560
                     /locus_tag="ACG8KN_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9189288.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="ACG8KN_002278"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="ACG8KN_002278"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP9189289.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="ACG8KN_002279"
     CDS             3650..4576
                     /locus_tag="ACG8KN_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFP9189290.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="ACG8KN_002280"
     CDS             4595..5845
                     /locus_tag="ACG8KN_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9189291.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1968 a          954 c         1200 g         1752 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagagaaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa attcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacaac aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agtttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gtta
//
LOCUS       DAZKCZ010000114         5535 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000114 DAZKCZ010000000
VERSION     DAZKCZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.117"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..348)
                     /locus_tag="ACG8KN_002281"
     CDS             complement(13..348)
                     /locus_tag="ACG8KN_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9189292.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(332..595)
                     /locus_tag="ACG8KN_002282"
     CDS             complement(332..595)
                     /locus_tag="ACG8KN_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFP9189293.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1055..1858)
                     /locus_tag="ACG8KN_002283"
     CDS             complement(1055..1858)
                     /locus_tag="ACG8KN_002283"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9189294.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1836..2960)
                     /locus_tag="ACG8KN_002284"
     CDS             complement(1836..2960)
                     /locus_tag="ACG8KN_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9189295.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(2976..3791)
                     /locus_tag="ACG8KN_002285"
     CDS             complement(2976..3791)
                     /locus_tag="ACG8KN_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9189296.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3781..4653)
                     /locus_tag="ACG8KN_002286"
     CDS             complement(3781..4653)
                     /locus_tag="ACG8KN_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9189297.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(4738..>5535)
                     /locus_tag="ACG8KN_002287"
     CDS             complement(4738..>5535)
                     /locus_tag="ACG8KN_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9189298.1"
                     /translation="LNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPA
                     DNWAQQAILESNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFI
                     DGNVAMVYTTIAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKE
                     ATWDFLKYLYSVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKM
                     VSWSSFPGDAGLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
BASE COUNT         1763 a         1143 c          855 g         1774 t
ORIGIN      
        1 tttttagtat tattataatt cttccatgcg ttcttccagt cgctgtaaat cgcttggact
       61 taattcccca atttttttcc atttgacctt tttgttgttt ttgggtaatt cgtttagagt
      121 tcctacgtca atccaagatt ctttttttag tcctgcctgt ttccaatccc ttatttcaac
      181 gtatttcagc tttattttat cggatttagt atgatatttt gaagtaattg acagaaacga
      241 aattgtgtcc agtccatttt ttagaatata gacaggccgg cgtttaccgc cctctgcagc
      301 tgtgaaagca acgtagacgg ttgctatatc attagtattc atcttcattg aaccattctt
      361 caattttttc gtcagtgtct agtttttgga caggcaattt ttccgctaat ctttgaatat
      421 tatggattcg tttacttttt tccgacaatt caatagaaaa tgggattttt ccttgctcaa
      481 caatttgctt ataaaaaacg gtgacggctg ttttggggct catgcctagt tcatcaataa
      541 tgctttctgc ttcatttttc aatttttcgt ccatagaaat ttgtaactta gccattttct
      601 ccacctcttt ctgacaaaca cattatatca cggaaagagt tcaaatggaa ttaatacaac
      661 tacttttgaa ctccttgaaa tgcagtgtat ttatctaaat ataaggaaag gatatagtgc
      721 taaatggcct tgatagatag aaaagtttca aaatccgcca cagcgataat tgaagcgaac
      781 actcaacgac acacagaaca ggaaaagact cctaaaagaa ccaaattaac taggacgtgt
      841 ctgaaaactc cacaaagatg tggtatgctc tggtaaaaaa cgaatggcac aaccacgaag
      901 accgatatgg ttcttggaaa actttctcca ccctttttgt aattggaggg atagtggttt
      961 attgatagcg attttccaag ttcttcaagt ggaaccagac attgagcacc acggctaaaa
     1021 agtgaatgaa gtctttattt ttaatcaata atttttaatg attaccagta attggttttt
     1081 tcatgcgccc tatttctatt tgatgtggag ttaaataatg ctgataagta aaaatttttc
     1141 cgtcaattaa actgccacta gtgaatcctg ttctattggt aaaaagagaa tagtctgggt
     1201 attcatctac accaaatgat aaagattcag ccataaactg ttttttacca gcaaattcag
     1261 cactgctacc ttgatggata tggcctacaa aaatacctaa aatatcactt tcggcaatca
     1321 attcagcaaa accttccgga acttcagtta caatagatgg atcttcccac gacaatggat
     1381 gatgaaccaa taaaatcgtt cccttttcag caggtgtacc caaccactct tgtaattgaa
     1441 tcatctgtcc tatcgatatt tttccttcgt gctgggtatc ttgagcagaa tccaaggaaa
     1501 taattcgtaa cccattttga ttcgtacaat ggatatactc tcccgtttca ttgctttcaa
     1561 cactcattcc ttcaaagaaa acactgcgac gatcatgatt cccacgacaa aaatagtaag
     1621 gtgtggttgg gaaatattga tccaacattt tccgtaagac ttgataatca gaaatccccc
     1681 cttcatgtac taaatcacct gttaatagaa taaaatcgta cactgttaac gagagttctt
     1741 gtaacaattt ttccaatgcc aactgtggat ttttaattgt tggaaatagt tcagcagtaa
     1801 acaagttagt ttgatagttt tctctaaaat ggatatcaga gagatgcaaa aagcgcatgg
     1861 ctattccctc tttcttcaat aaaataatca ggatatccta aattttcttg cgtctgataa
     1921 tcaaaaaaat gcattctttc aggttctatg cggagattga ctgcatcacc aatctgccaa
     1981 gtaggttttt cattaaggac aatcatttct ataccatcga ctttaacggt gtacgcataa
     2041 ctttgaccta atagctcacg atacttcact tgaccgtgta aatcgcttgt ttcttttgaa
     2101 aaggttacgt gttctggtct tatgcctaaa tacaatgagc cactaacctg aagaagttgt
     2161 ttttgccaag ctggattcaa tgttaaaaat tgactggcta cctttaatgt tccttgatca
     2221 taagtaactg gcataatatt catgcctggc gaaccaataa aatttgcagt aaaagtattt
     2281 ttaggtttcg tataaacatt tttcggcgta tcaatcatct gaattctacc ttcattcaat
     2341 aaaacaatcc ggtcagctaa cgtcatcgct tcaatttgat catgagtgac gtagacaaaa
     2401 gtttgtttat aacgttgatg aatttttacc aattcttttc gtgcatccaa tcgtaaacgc
     2461 acatccaaat ttgataacgg ttcatctaat aaaaaataat tactccgttt aaccaccgca
     2521 cgagccaatg ctacacgctg ccgttgccct cctgacaaat ctttaggaag tcgatcttgg
     2581 tatggcgtta aacctaacat cttcaaagct tcttccaaac ggtgctgaat gtcagctgtc
     2641 ttaattttat tcgcccttaa tgcaaaaata atattctcct ttacagtcat gtgaggatat
     2701 aaagcataat tttgaaaaac cattgcgatc tctcgttctc cacaaggtag cttatttgct
     2761 agttgtgtac ctaaaaaaag ctctccactc gtaatttttt ccaaaccagc aatcattctc
     2821 agcaaagtag attttcctga accagatggt cccagtaaca caattcgttc tccagcggcc
     2881 acagctaaat caatttcttt taaaatggta tgttctccat atttttttcc gatatttctt
     2941 aattcaagcg tttctcccat gatttgtcct ccaatttatt taatacctgc agaaacaaaa
     3001 gtcccgataa tatatttttg aaaaattgca tacaagacta acggtacaaa cattgtaatt
     3061 aaactaatcg ccattgctac ggtaaaattt gtcccacctt cagaactaat ataggtctgc
     3121 atcgccaacg gcaaagtata attatctact gtccgtaaaa ttaataccgg ccaaacatac
     3181 tcattccaag aggtaataaa aaaccaaata ccggtagaag taatcgcatg tctagtaaga
     3241 ggaaaaacaa ttttactcat gacggttgtt tgtttgatgt tatccaatgc cgccacatct
     3301 agaaggctca ctggaatttc ccgcattgtt tgcgtcaata aaaaaatgcc catcgcatca
     3361 gcaaattgtg gtattatcac accccaagta gtatcaatta aattaagttt tgcaatcaac
     3421 aaataattag gaatcatcgc aacagtaaaa ggaatgaaca tcgtaccaat gatgataaag
     3481 taccagattt tcttcccttt gattcggcaa taagtaaaac tatatgccgc aaaaaaagct
     3541 acgcctaact taacaaccgt tataattgta gcaataaaaa aagtatttct cgtaattttg
     3601 ataattggaa gattttcgaa caaatagcaa aaattttcca aggtaaacgg ctgtggtatc
     3661 aaactagtaa tcgtctgata ggcatctttc aatggcttaa aagaattggc cataataaac
     3721 aataatggat agataataat gattatgatg aacataaata agagatgtgg caaccagcgt
     3781 ttaattttca tagtaaaccc ccttttccac aaatttaaat attaccccta agaaaacagc
     3841 aaatattaat aaagttaaaa ctgaaattgc tgaagattct ccgactttat acatttcaaa
     3901 agccttttga taagcataat agatcacgtt tgcactacca ttattcggtc caccacgagt
     3961 gatgatctta atcggagtaa acacatactg cagaccttga acaatggtca tcaccaataa
     4021 atatagtcct gtcgaactac tcataggcaa cacaattgta aaaaaaatgc gccagatggg
     4081 tacacgatcc aacttagctg cttcgatcat ctcttgatca attcccacaa ttgctgcaaa
     4141 aatgacaatg aaattataac caaaaacttt ccaagcagta atacctgcaa tcacagcaat
     4201 tacccaacct tgtaaattca tccacatagg cagtcttaaa ccaaaatttc ccaacaagac
     4261 agcaattggt cccgataccg gatttaatat ccatgtaaaa accatccccg ccactaccaa
     4321 agaaatcaca gctggaataa aaaataatgg tttatacaaa ttctgccctt tttctataac
     4381 tattcctaat aaaaaggatg taatatatgg gaacacacag ctcagcaaga caaatagaac
     4441 ggtataggac aaagtattct tcaacacttt atgaaagact ggatcagtca gcacctttac
     4501 atagttggca aagccaacaa attctttagt gggggccacc atattccagt caaaaaaact
     4561 caaatagatc gtataaatca atggataaaa aacaaacacg cccaacaaaa taattgctgg
     4621 cgccagatac aaataaccta catagcgctt catttccgac agcctcctca tccttatgtc
     4681 aaaagcagac gaccttgttt ttcctttctt acaggcgctg tcgtctgctt gctttgctta
     4741 tttgagtaac tcattaattt ccttttgagt agctttcatc gtttcattga cggaattacc
     4801 tgataaaatt ttatcccgca tatccaacaa tttttgttca gcttctaatc cagcatctcc
     4861 aggaaatgaa ctccaactga ccatcttatc catctgatca attgctggta tcatcatctg
     4921 attttcagct aagaaagctt ttaatccatt ttgggcgtct gcaacatctt tacgtggtgg
     4981 taaataacca gtaccttctg tccatttagc taaagattca acactataaa gatattttaa
     5041 gaaatcccaa gttgcttcct ttttcttatc cgtttgtgat gtaacagcta acattgcgcc
     5101 accagcaggt agtttaactt ttttcccttc ccaacttggt gactcaactg ctttagcagt
     5161 aaaagtacta tttttttgaa tgttgtccct ttgagcaata gtggtataga ccatcgcgac
     5221 gttaccatca ataaagcttt gcacaccttg ttcccacgta gtgtgtaatg ccgtctggtc
     5281 ttttaccatc ctttgatata attcataagc agcgatacca tctttagaag caaaggtagc
     5341 tttgccatct tttattacac ttgccccatt actttctaaa attgcttgtt gtgcccaatt
     5401 atcagcaggt tcttgaatat acacgccata ctttccagtt ttttctttta ttatcttaga
     5461 aaaagtttga acttcttccc aagtttttgg accattttca tccaagcccg ctgcttttaa
     5521 tatatcttga tttaa
//
LOCUS       DAZKCZ010000115         5516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000115 DAZKCZ010000000
VERSION     DAZKCZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.118"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..1097
                     /locus_tag="ACG8KN_002288"
     CDS             15..1097
                     /locus_tag="ACG8KN_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFP9189299.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1097..1900
                     /locus_tag="ACG8KN_002289"
     CDS             1097..1900
                     /locus_tag="ACG8KN_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9189300.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            1970..2710
                     /locus_tag="ACG8KN_002290"
     CDS             1970..2710
                     /locus_tag="ACG8KN_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9189301.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            2721..3725
                     /locus_tag="ACG8KN_002291"
     CDS             2721..3725
                     /locus_tag="ACG8KN_002291"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFP9189302.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(3770..5515)
                     /locus_tag="ACG8KN_002292"
     CDS             complement(3770..5515)
                     /locus_tag="ACG8KN_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189303.1"
                     /translation="MAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTI
                     PTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSL
                     AVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIA
                     VFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFL
                     SHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPI
                     ANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTIN
                     WSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNT
                     LRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQK
                     LAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHE
                     QEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFI
                     DRITVQLSRNYPNPV"
BASE COUNT         1764 a          916 c         1132 g         1704 t
ORIGIN      
        1 atgggggaaa acatttggat acaaaagaag aacagctact tattgattta acttctgcaa
       61 aaggagtacc tggaaatgaa gaagaggtaa gagaagtatt cagagaatat gccaaaccat
      121 tcgctgatga tattttttat gacggtttag gcagtgtgat tgccaaacat ggtgctggcg
      181 gaggccccaa ggtctttatt tctggacata tggacgaagt tggttttatg gtaacaaaaa
      241 taaccgaaaa aggattcttg gaattccaaa ctcttggagg ttggtggggg caagtcatgc
      301 ttgctcagca agttgaaatc aaaacacgtg aagggaaaat cgtccacggt gtgattggtt
      361 ccaaaccacc acatgtttta acacctcaag taagaaataa accttatgaa ataaaggata
      421 tgttcattga tattggcgca tcaagccaag aagaagccaa agagtggggc atacgtcctg
      481 gagatatggt cactccatat attgaataca aacgaatgaa tggcagtaaa tatttactgg
      541 caaaagcatg ggataatcgt atcgggacag ctgtttctct gcgagtatta gagaatctat
      601 ctaaagaagc acacccaaat gttttattcg caggaagcga tgtgcaagaa gaagtaggct
      661 tgcgaggtgc tcgaacaagt acacaccttg taaatcccga tattgctttt gctttagata
      721 ctggtacagc aggagacact ccaggaatga caccaaaaga agcagattcg attttaggaa
      781 aaggcccaca gattttgatt tttgatgcat ctatgatccc gcataaaaaa ttattgaatt
      841 ttgttattga tgtagcagag gaaatggata ttccattcca atatacagtg atttctggcg
      901 gaggaactga tgcaggacaa atgcatttga caagagatgg tgttccgtct gtagccatta
      961 ccgttcctgt tcgttatctg cattcccata catcagtgat tcatgaagat gattatttga
     1021 atacagtgaa actagtcaca gaattagtga aacggcttga tagtgaaaca gttgaaaaaa
     1081 ttcggagtta tgaataatga ataatagaat acctatttct gaatatcgga aaaaagcacg
     1141 agtttctttg gaaaatcaat ggggaatcaa tacttggctt gttttcttat ctataatatt
     1201 tacgagcgtc atccagaact tttttgacgg tttcagtcct tttccggaag aaagcatgca
     1261 gcagcactta ttgagttttc tagtcgataa tttactttta ttcgcattca actatgcatt
     1321 gttttacatt gcgctagtag ttgtacgggg tggcagacca aaaagtaacc ttttattttc
     1381 agttttccaa agaaagtatt ttggttcgat acttggcatc aatttgatca attcagtcat
     1441 caactggcta ctcaacagtt taatctttct gcccatgttt ttaatcagcg gagtgagtac
     1501 ttatatgcag ctgcttttca gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt
     1561 tcttgatatc agctttgttt tatcgatgat agggatgatt tttgtttcag cactcatcat
     1621 gagtattgtt ggcggtttat tccaatttgc tgcatggaca aagttcgact atcctgattt
     1681 gtcggttatt caatgtttga aatatggctg gtttttattg aaagatagaa ttggtacgta
     1741 tattttgttg cagctgtctt ttatcggctg gtatttgcta ggagcaatcg ctttgttcat
     1801 cggtcttttg tgggtcattg tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc
     1861 acggattgaa aaaggaggtc cagacgccta ttttacttag catgtaagca gtttattcat
     1921 caaaaaaata acttttgtgg taggataaat ctttgagggg gaatgtacga tgaaattaac
     1981 tgttttagga tgcttaggag cgtatcctta taaaaatcaa gggacaacag gttatttgct
     2041 tcaatctgac gggttcaatt tgttgatcga tgcaggtagt gctacgctga tcaaattaca
     2101 agaacacctt gatccacttg atctagatgc tgtgatcctt agccattatc atcatgatca
     2161 tattgctgac cttggtgtat tacagtatta ctggcaactt catcctaaga gaaatccgga
     2221 gacattgctt tcaatttatg gacacacaaa agattccatt cactttgaag aattgacgtt
     2281 ggaaaatgtc tcaaaaggac atccttattt tgaagctgaa gaattaaaaa ttggtccttt
     2341 tctgattact tttatggaaa caatccatcc agtagtatgt tacgccatgc gtatcgtaga
     2401 agaaacaacg gggaaagtat tcgtatttac aggtgattca ggttatttgg aaagtttcgc
     2461 tgattttgcg aaagatgctg atttgtttct agcagatacc tatctttttg aaggaaatga
     2521 acatcatcat gcccatttca cttcaaaaga agctggagaa cttgcgaaat cagctaacgt
     2581 aaagaaactt gtacttacac acctacctca gtttggtgat ttagaacaat tgaaaaatga
     2641 agcgcaaaag gcagcaggca aagggattga tatagaactt gctgcagttg acaaagtgtt
     2701 taacatttag gaggaagaac atgatttttg taccaaatga aaaccacgac ccaagaatca
     2761 atttagcaat tgaacaatat ttgctgcaag aaatgccttt agatgaacct attttgctct
     2821 tttatatcaa tgaaccttca atcatcatcg gcagaaacca aaatacgctt gaagaaatca
     2881 atcgagatta tgtggaagaa aatggcattc atgttgtccg tcgcttaagt ggaggcggag
     2941 ctgtctacca tgattttgga aacctgaact ttagttttat catgcctgat gatggggatt
     3001 cattccgtga ctttgcgaaa gtaacacagc caatcatcca agcacttcat gaattgggtg
     3061 ttgctggggc ggaattgaaa ggccgaaatg atctagtcat tgatggcatg aaattttctg
     3121 gcaacgcgat gtatgctacc aacggtcgga tgtttgctca tggaacaatt atgttcgact
     3181 ctgatatcaa tgaagtagtc aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag
     3241 gaatcaaatc gatccgttct cgtgtaacga atatcaaacc atttcttcca gaagaaaagc
     3301 aaaaaatgac aacagaagaa tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt
     3361 cagtagaaga agtaaaaacg tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa
     3421 tctctgagga atattatcga aattgggatt ggaattacgg gaagtctcca gatttcaatt
     3481 tctctcgcca aaaacgtttt tctatcggtt cgattgaagt ccatttgaat gtctcagaag
     3541 gaatgatcaa agaagcaaaa atctttggcg atttcttcgg actaggagat atctctgacg
     3601 tggaaaaaaa attggttgga cagaaatatg ataaagcatc tctatcaaaa atagtcagcc
     3661 aaattgatgt gaagaaatat tttggtgcga tagaaccaga agaattgctt accttacttt
     3721 attgaaaata aaaaatagcg gaatccacat atatggattc cgctattttt tatacaggat
     3781 tcgggtaatt tcgagagagt tgaacagtga tccgatcaat gaaagtatct ttttcctgcg
     3841 ttagtgttcc tgtgtaacga tccgctacgt ctttcatcat cattaagtca aaacgttcct
     3901 gatcatctaa ttcttctgtg atgcctcgac taaataggtt tgtatcgtaa tgtgcactat
     3961 gttcgcaaca aatgacgaaa tgttcctctt gttcgtgtat cgacaaataa atttttcgat
     4021 acagtggatt ttcgatggca tgagtttctc taaaggaatg ctctaaagcg tggatcaaaa
     4081 gttgagccag atcgtcatcg ttgataggaa gttcttcggg aagatctgct tggaaggtta
     4141 tctgaatatt ccgtttggca gctaacttct ggtaacgcgc taagataaga tttgtcacct
     4201 ggtggttgca aaaagaacac agcatttcga aattttttgc ttcttctaat atcttttcca
     4261 catagttttt agcttttgtt tggtccgtat gcattaaatc ttgcagattc tccatctctt
     4321 ggacaaattc caggcgaagt gtatttactt cttggatatg ctgtcgaagc tgctcataat
     4381 gttgctccaa caaatcattt ttgattttta agaaatctac ttgtctccga tctttgtcgg
     4441 tatttaagat gtgatcgatt agattgtagc tgataataac gattagaatg ataatatata
     4501 aaatcgtaga ccaattgatc gttgtttgat agatacctaa tgccgtttgg atgtaaatca
     4561 agcaatggat aatggctacc aagacgatcc aaggtgtaca agctacataa aaacggttat
     4621 tcttatatgc ttcgccaatg catgcaagtg aagtgactaa agtgcttata acactagcaa
     4681 tattagcaat cggcattaat tcaggaagtt ctgcaggagt aaaagttgca taaagcagaa
     4741 cgccgataaa aatcaaaagt tggaacgtac aaaaaattcc gtaccatttt ttatagtaaa
     4801 acattcggct ccaatagtag cagatgatga aagctgacgt taagataata aataaatgag
     4861 ataaaaaaga atgaacgaat ggttcaaaaa gaataccact taaaatatct tctgatacag
     4921 cttcaaatcc taatgccaaa gcaaagcaac ttaagagaat aagtgagtat tcaagacgtt
     4981 tttgtttata catcaagtag atcgtataga aaaaggtccc tatagcgata aaaacagcga
     5041 ttatcatcgt caatacttgc ggcactgatt gtgaataaat aaaactaatc aatggtccag
     5101 ttgttcctag ataaactttc ggtggcagtc cagcatagtt ctgataagga gatgagactg
     5161 taagggtcaa tttttttccg atataatttg ctggtaaatc tatcacagcc aatgagagtc
     5221 cgggattgct ttcttcctga taatctttcc gttggtataa aatcttttcg cctagatata
     5281 cagtaatcca ttgatggttt cctttgatta gtaaatttgc ttcgtccaac ggtttttcaa
     5341 gtgtcgtttc cataatgaat gtttcgtctt tatccacggt aggaatcgta cgagtgtact
     5401 tgctgtcaaa cttaaaatca ggatcatctt ttgtatagaa aacccacgaa tcttttattt
     5461 ctttcatttg attgctgtcc gcaggagaaa aaataaaata aaagaaataa gcaatt
//
LOCUS       DAZKCZ010000116         5504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000116 DAZKCZ010000000
VERSION     DAZKCZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.119"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(201..569)
                     /locus_tag="ACG8KN_002293"
     CDS             complement(201..569)
                     /locus_tag="ACG8KN_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFP9189304.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            719..1201
                     /locus_tag="ACG8KN_002294"
     CDS             719..1201
                     /locus_tag="ACG8KN_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFP9189305.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1238..2248
                     /locus_tag="ACG8KN_002295"
     CDS             1238..2248
                     /locus_tag="ACG8KN_002295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFP9189306.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2363..3076)
                     /locus_tag="ACG8KN_002296"
     CDS             complement(2363..3076)
                     /locus_tag="ACG8KN_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189307.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(3249..3856)
                     /locus_tag="ACG8KN_002297"
                     /pseudo
     CDS             complement(3249..3856)
                     /locus_tag="ACG8KN_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            4379..5054
                     /locus_tag="ACG8KN_002298"
                     /pseudo
     CDS             4379..5054
                     /locus_tag="ACG8KN_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            5047..5208
                     /locus_tag="ACG8KN_002299"
     CDS             5047..5208
                     /locus_tag="ACG8KN_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189308.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
BASE COUNT         1906 a          870 c         1011 g         1717 t
ORIGIN      
        1 aaataatttt actaagtgga aaattcaaag ccattcaatc agtgattttt ttcagcacaa
       61 gaaagactcc ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat
      121 aaaaaggata ggattgaaca aaacctgtat aagctgatga atagtttcct cgccagtaaa
      181 aggtttattc gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata
      241 ttaaatgaaa tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg
      301 atgacgttat ctgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc
      361 gctgcttttt ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag
      421 gtaccatggt cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac
      481 gtttctgtta tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatc
      541 tgattgatga tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt
      601 attttaccag aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta
      661 accgctatat ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat
      721 gattgacttg gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg
      781 agccatttat acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac
      841 agagaccaaa agcttattct taaaagataa aaaagataat aactacgtat atctgacttt
      901 tactacgaaa caaacggatt tcaaagcatt ggggaagatc gtcggtaaaa ggctttctat
      961 tgtttcatca gaacacatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt
     1021 tggttatgaa aaacagattc cgattattat tgatgaagag ctcttattaa atgaaaaact
     1081 tgtttttgca ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa
     1141 aatcatccag ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta
     1201 accaagtaaa atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga
     1261 aagaaataca acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc
     1321 gttactcgaa tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa
     1381 gacgctttgg acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt
     1441 caatcaaatg atgcaagatg aaaatatcca aatgatcttt ccgccttggg gaggagaatt
     1501 attgattgag attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg
     1561 ttattcagat attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc
     1621 acatggaatc aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg
     1681 ggaaagtgta ctgaaaacaa gagaaggaga atcgattcta cagcaacctt ctgaaaagta
     1741 tcaaaaagag tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac
     1801 aaattggcaa tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggctg
     1861 tattgatacg atcagtcatt taatcggtac cccttatgga gatgtccaat cttttaaaag
     1921 aaagtataat gatggaaagc cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt
     1981 tgatttaaga agatcacttg ttcagatgaa actggcagga tggtttgatg gcagctcggg
     2041 gatcatgttc ggtagaagtg cagcagagga tttgttagat tataaaatga aggatgtata
     2101 cgaagatctg tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt
     2161 accgccacaa ctgacattga tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa
     2221 agcgactgtt ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tttatcaaag
     2281 accaacaaat ctaaatagaa aagaaaatga aagaagctga cagtattttg ccagcttctt
     2341 tttgtatgat ctattagaaa ttttaattat ccataactat cacattgatc agctgtttca
     2401 catcatttgg attacctgta ggaatagcta ctacagatgc attatcaata atttcttgag
     2461 ttgtttctct aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgtag
     2521 gaactggata actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt
     2581 aactgtattt cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat
     2641 aaggggcgat aggtgttcct gcaggataat gaataacatg tagttcgaaa tcactcgatg
     2701 tatctctaaa agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat
     2761 cagaatatcc tgctaacgga tctattgact gagaagactg tgaggaagtt gtgtctattg
     2821 ttgaagaaga aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg
     2881 agactttact gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg
     2941 atttttcagc tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac
     3001 tattttcttt actaggagac gtacatccta caaggaataa agagaccaac aataaaataa
     3061 aacctaactt tttcatataa aatacctcat tattttttta ataccagtat agcgtaatcc
     3121 tgaatttttg aatccgtaaa accttgtcta tgaatataaa tagagtatat cttctttgat
     3181 aaatgtcaga aaaagtgtga caaataacca tcagtaaaat tacttgtcat actattgaga
     3241 aggagtattt aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat
     3301 catagaatcc aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat
     3361 ggactgcttg aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca
     3421 aattacgatc tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg
     3481 attctaacca aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct
     3541 gagctaaata atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt
     3601 tatcgattat ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa
     3661 taaaaaggtc aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc
     3721 ctccaatgga agtgacctct gattggatag aagcagtttt gttttgccat ccctcttttt
     3781 ggtttagtgt gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg
     3841 taatcgcagt tttcattttc aagctctcct ttactatcta tgacaaggac aacagtttta
     3901 acatacagac attcattaaa tttggatagc gacagcgaaa acaacaattc ttaagtttta
     3961 gacagagtat acagacagtc aacttaatta aatacgaact aaataaaaat gacaggtaat
     4021 catttttgtt taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc
     4081 cacttgcttg agtacaatct taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa
     4141 aaataaatca tatcgggcac tcagacatga aaacaaccct agaaatatat aactagtcca
     4201 atgaaaaaac agcgaatgcc gtagaaaaca aggattctgc gtacattcat atctcatttt
     4261 gcgagcatat ctgctattac tgtgactaaa tgttaatatg gttaaaatga ccgttatatt
     4321 ttttatataa atatcgtata attagtatga atatagaaga aggaaagaag gtgcaatctt
     4381 ggaacctaaa gacaagtatt tagattacgc tagggtcaat aaagataaat ttattgaaac
     4441 aatcatcaag ggaaaaatcc ctgatgatga aaaagatgcg gtattcatgg ctggaagccc
     4501 aggagcaggt aaaacagagg ttgcactagg tttagctgaa aattatgaca accatgttgt
     4561 gattgatgcg gactatttta gagcacaatt tccagaatac aatggaaaga actcaagtgt
     4621 ttttcagaaa gcttcttctt ggttagttga acaatcatta aaatatgtac tggaacatgg
     4681 ctattctttc atactagagg atacttttgc tatccttagt gcagagaaaa atattattcg
     4741 agcccttaaa aataactttc gcgttacgat tttctatgtt taccaagatc ccaaagttgc
     4801 ctgggatttc actcgaaaac gtgaactagc tgaaggacgt catgtgccaa aagaaatgtt
     4861 tatcaatgcg ttttttaagt ccagagagaa tattgaaaaa gtcaaaaata gacatcctga
     4921 agtagtcctt cacataatga ttaaggatta tcaaaacgac atttctgaag tgcattatgc
     4981 agcagataat attcaattag tactacccaa tacaatatac ttcaaaagag ttggaggagg
     5041 aattacatga ctgagaaaga acaagtacaa caaatcgtga agaagtataa taaaagtatc
     5101 gctgatttat cagaaaacgc ctcagcgaaa gaatttaaaa cagtaatgaa gtacgtagca
     5161 gatgaagcaa atcgtaagca gcgaaaacta gttgggttgg ataaataaat tttttaaacc
     5221 atatccttgt aaagttgagt tttttgtttt aatgaattaa gactattaaa tagataatta
     5281 ttgtaactta aaaaacacag ctaaaaatta gttgtgtttt ttattaggtt tacttttctg
     5341 gtataaaagg aactagctac tttttcaaaa tttctaaatc attcaatatg tagcgcttac
     5401 taaattattc gccataaaat tttataattg gatcactcat tgttttacct aagtcatatt
     5461 taagtaataa ccggtgtttt tacataagaa gacttggggt attt
//
LOCUS       DAZKCZ010000117         5359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000117 DAZKCZ010000000
VERSION     DAZKCZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.120"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(321..434)
                     /locus_tag="ACG8KN_002300"
                     /pseudo
     CDS             complement(321..434)
                     /locus_tag="ACG8KN_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(817..2049)
                     /locus_tag="ACG8KN_002301"
     CDS             complement(817..2049)
                     /locus_tag="ACG8KN_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9189309.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2303..2872
                     /locus_tag="ACG8KN_002302"
     CDS             2303..2872
                     /locus_tag="ACG8KN_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9189310.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3050..3490)
                     /locus_tag="ACG8KN_002303"
     CDS             complement(3050..3490)
                     /locus_tag="ACG8KN_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9189311.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3648..4412
                     /gene="map"
                     /locus_tag="ACG8KN_002304"
     CDS             3648..4412
                     /gene="map"
                     /locus_tag="ACG8KN_002304"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9189312.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4444..>5359
                     /locus_tag="ACG8KN_002305"
     CDS             4444..>5359
                     /locus_tag="ACG8KN_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFP9189313.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHK"
BASE COUNT         1658 a          977 c         1038 g         1686 t
ORIGIN      
        1 tttgataaat ctattcgaag ggtcaaattt aaaccataat tgtgtttttc cttccatcag
       61 cataaaagtt cacctcaaaa agagtatacg aacgaatgtt ctttttgtga aggcggaatt
      121 taatatactg aataatagaa aacaagtact tgtgccaaat tatgtgccaa aaaaattcga
      181 attcaacaaa atgtaaacaa atagaaaacc ttctatatta agatttcctg caataacaaa
      241 cacaacagaa gacgtgtaat agttagtcag gaacgtacaa ataacccctg tatcctttgc
      301 ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct atgtcctaaa
      361 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgcgtatgt
      421 cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac taaagaaaaa
      481 cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat taaacgattt
      541 cgattggctt ataccagtcc tatccaacga aagatatatc gttcttaact ctagcggtat
      601 aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt tattattttt
      661 agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat ataaaaaaac
      721 ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct ttattctatg
      781 ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa
      841 aatcggtact gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac
      901 catgaaatct acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga
      961 catttctgta ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga
     1021 tgcgttttca tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg
     1081 gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt
     1141 tgctaagaca tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt
     1201 aaatttcata cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta
     1261 accatctact cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat
     1321 gaatttttct ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa
     1381 tccgataatg atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt
     1441 caattcttct gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc
     1501 ttcttcatag acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc
     1561 ttctggcagt tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt
     1621 ccaactgact ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc
     1681 tgattgataa gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga
     1741 aacaacgctg atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga
     1801 ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg
     1861 gtcgtcagtc cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat
     1921 caatcgagca aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc
     1981 tgtggctacg ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc
     2041 agataacata ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca
     2101 tttaattatg attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt
     2161 taagtttcat gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta
     2221 attttgcata tcaaaaaaac atgttacaat gaaattaatc aatggtggaa gcgttataaa
     2281 aaaattaagg agattaaaaa atatggcaag aaaaaagacg attactagag aacagatttt
     2341 ggcagctgct tatgaagtag ctgccaccga tggatttaca cgatttactg cgagaaacat
     2401 cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt gagtttaaaa atatggatga
     2461 cttgagacaa gctctttttg agcagatcta tgatcattta caatatgaag tgttcactgt
     2521 tgagcataca gggaatacga ttatcgatct tgcttttaat tatatccatt ttgcaagaag
     2581 agagaagaag ttgtatcgtt cactgtactt agaagaatat ggtggaggaa aagagatgca
     2641 gaatttctct tatacttact ttgtgaatac agtgaaacgc gatccagctt atgtgaatct
     2701 ttcagaagag cagatcgaat ctttgtataa tgggacatgg atcatagcta ctgggatcgc
     2761 agctttgatg tcttcaagca tcatccatcc aactgatgaa cagattgaaa agatgatcca
     2821 agacagtata gatgctatct tgaaattcaa agaaccaatc gaaatcgatt gaatatactc
     2881 aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa aataaggaaa agttcgtttt
     2941 tcttacagcg tatgcatttt taactcccga agaactcttc catgaatgca atgaataaaa
     3001 gagccataca gattgaataa atctggatgg ctcttttatt gcaagtatat taaatttttg
     3061 cgactagttc tttagcgaaa gcttctaaat tttcgatatc ttcttcttca gcattcagat
     3121 ccactttaac attttcggct ccttttgatg cgcctgtttt tgcaaaagca gcatcgaaat
     3181 catccaccga tttgcagaat tcatcgtaaa aagtatcacc agatccgcag acaccgtaaa
     3241 tttttcccaa tagatccaat tcttgcaagt cttcataaaa gtctacgatc tcatcaggca
     3301 gatcgccatc atcgtatgta taagtagcta ctacgcaaat atctgcctcc tcgaattcat
     3361 ctgcatctac ttgggtacat tcgttgattt cgacttcgat atccagattt tcaaatgctt
     3421 ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat gctagcatat acgatttttg
     3481 ctaaagccat gatgtgcctc ctcgtttata attatttaac aattatgaat tataccaaaa
     3541 catcattgaa aagaaaataa aacctaaaga cacctgggga atgtcatttg gctaaatgta
     3601 tgccgctatg ttaaaatgag agggatttgt aaaaaggaga cgaaaatatg attactttaa
     3661 aatcagcacg tgaaattgaa atgatggcag aatctggagc tttattagca gatgtacata
     3721 aaaatctgcg tgattttata aaaccaggaa tcacaagttg ggatattgag gtatttgtca
     3781 gaaactatat cgagagtcat ggcggaattg ctgcgcaaat tggctttgaa gggtatgaat
     3841 atgcgacttg tatcagtata aatgatgaaa tttgtcatgg attccctcgt aaaaagcctt
     3901 tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga tttgaaaggc ggcatttctg
     3961 attcttgttg ggcctatgta gttggagaat ctacacctga gatcgaccga ttgatggaag
     4021 taacgaagaa agctttgtac atcggtattg agcaagcaca agtagggaat cggattggtg
     4081 acatcggtca tgctatccaa acttatgtag aaggcgaaaa tctagcgatc gttcgtgatt
     4141 ttattggtca tggcgttggt cctacgattc atgaagaacc agcagttcct cattacggag
     4201 aagctggaaa agggttgcgg ttgaaagaag gcatggttat cacgatcgaa cctatggtta
     4261 ataccggaac ttggaaaatg aaaatggatc ctaacggctg gactgcgtat acaagagacg
     4321 gcggactttc ttgccaatat gaacatacat tggctattac taaagatggt ccaagaattt
     4381 tgacatcaca aggagaagaa ggcacttatt gatctatcca gtcaataggt acgggagagg
     4441 tacatgaaag tttggaacaa aataaaacaa aatgaaaatc tgatgcgctt tattgagacg
     4501 actcaaaagc gcatcactga ttcagaaatg agtacgacct ctgtcgtcgt tgcttattat
     4561 ttactcttgt ctttatttcc attgattatt gcttttggta atattttacc gtttctccac
     4621 atcgatcaag agactgtact gacctattta cgagaagtga ttcctgagac cgtttaccag
     4681 tttatcggtc ctgcaatcaa agaccttttg acacaaagtt caggaggctt gttatccgtt
     4741 tctgcattag cggctttatg gtctgcaagt caaagtatca atgctttgca aattgcgatg
     4801 aataaagcgt atggcgtaga aaatagaaaa aattttttga tcgtccgttt cttctcacta
     4861 attgtcattg tcttgttcat gattgctatt agtggggtaa ctatcgttct tgggttggga
     4921 cagctgattt tggatgctat tcagccaatc gtacaaatac ccgtgaactt tatcgatcag
     4981 ttccaaactt tgaagtggcc aatcacattg gtagctttat ttgtcatcat gttcttgatt
     5041 tatttaattg tccctaatgc tcagttgaag ttgaagacga tcattccggg cacgatcttc
     5101 gctactgtcg gctggatgct gttatcacaa gtgtttagta tctatgctag gtattttgca
     5161 acgaggatca gcggttatca gatcatagga agttttattg tactcatgct ttggcttaat
     5221 tttgctgcta cgatcatcat tttgggcgga atcatcaatg cagttgttca agaatacgtg
     5281 acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa aatggttcaa taaattgaaa
     5341 actaaatttt ctcataaaa
//
LOCUS       DAZKCZ010000118         5177 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000118 DAZKCZ010000000
VERSION     DAZKCZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.121"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..2100
                     /locus_tag="ACG8KN_002306"
     CDS             166..2100
                     /locus_tag="ACG8KN_002306"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9189314.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2557..2973
                     /locus_tag="ACG8KN_002307"
     CDS             2557..2973
                     /locus_tag="ACG8KN_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFP9189315.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2987..3553
                     /locus_tag="ACG8KN_002308"
     CDS             2987..3553
                     /locus_tag="ACG8KN_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFP9189316.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3576..4562
                     /locus_tag="ACG8KN_002309"
     CDS             3576..4562
                     /locus_tag="ACG8KN_002309"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9189317.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4717..>5177)
                     /gene="scm"
                     /locus_tag="ACG8KN_002310"
     CDS             complement(4717..>5177)
                     /gene="scm"
                     /locus_tag="ACG8KN_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP9189318.1"
                     /translation="SSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDST
                     DTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLT
                     NKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         1587 a          947 c         1132 g         1511 t
ORIGIN      
        1 atttcgtgaa aaaacgcttt attttaaaat ttgaaaaaaa tccaaaagga gttagacaaa
       61 agaacagaac atctatataa tatatggaga ttgtgtaata agtatcaaac taaaagaatt
      121 atcatatatt cattatatag atagtctata aggagcggat gatcaatggc aaagcaaaat
      181 attgtcgttg taggagcagg atatgccggt gtttcagcta caaaatactt ggcaaagaaa
      241 ctgaaaaaag aagatgtagc aattactctg atcgatcgtc attcgtacca tactatgatg
      301 accgagttgc atgaagtagc tggcggacgt gtagaaccag aagcaatcca atacgatctg
      361 cagcatttgt ttgctcgtca aaagaatgta caacttgtca cagacactgt aatcggtatc
      421 gataaagaac gaaaaatcgt caagaccaaa ttaggttcct atccttttga tcagctgatc
      481 cttggaatgg gcggagagcc gaatgacttt ggtacacctg gtgtcaaagc acacggattc
      541 accctttggt catttgaaga ttctgtaagg attcgggaac atattgagaa gacagtagca
      601 aaagctgcat tggaaccaga tgatgaactg cgtaaagcca tgctgacttt cgttgtctgt
      661 ggttctggat ttaccggtat tgaaatgatt ggggaactga tcgactggaa aaatcgccta
      721 gccaaagatt ataaattaga tccaaacgaa atcacactga tggtcgtaga ggcaatgccg
      781 acgatcttga atatgttgga tcgaaatgat gctgcaaaag ctgaacgcta tttgcgtaag
      841 cacaacgtgg aattattatt gaatgcacca atcgtagaag tggctgctga tcatatcaaa
      901 ttgaaagacg gacgcaacat tccgactcac acactgattt ggacagctgg tgtcaaagga
      961 acttctgacg ctgctgactt tggattagaa tcagcacgcg gacagcggtt gatcgccaat
     1021 gaatatatgc aggcaaaagg ctacgaagat aaaaacatct atatcattgg tgatttagtc
     1081 ttttatgaag aattcccagg aacaccgaca ccgcagatcg ttcaagctgc tgaacaaaca
     1141 ggacacacag ccgcagcgaa tatcgtggct tcgatcaaag gcggagaaaa gcataaattt
     1201 aaaggaaact atcaaggatt tatggtttct attggtgcca aatggggggt agctaaccta
     1261 tttgataaga tccatttaag cggattttta gctatcgtaa tgaaacatat cgtgaatttg
     1321 aaatacttct ttgatattcg ttctggttat tatatgttcc aatatttaat gcatgagttt
     1381 ttccatatca aagatgatcg caacgtgact cgaggacatt cttctcgtta cggcaatgtc
     1441 ttatggagtg tgccacttcg tattttctat ggtttagttt ggttcgttga agcttccaaa
     1501 aaaatcattg ggaatggtga agtgttcaaa ccaagtactt ggtttggtga aggatcatgg
     1561 ttcactggaa acagtgtgtt cccttggaca tgggaataca atgtaacaga tgttgccagt
     1621 ggtgcttctg ctgctggtga tgcaaccaca gctgccagcg gagcaggggc aagtgccgga
     1681 gaagcagcga ctcaagctgc acattttggt ttgagttatg catacggtga agaaccaatg
     1741 gctgtgattg agaagtcacc agactggttc acaaagatca tgcaagtgat catgccaaac
     1801 aatgatgtag caatgttctt ccaaaaattc atggtattct ttgaaattgc cttggcatta
     1861 gcattgattg ctggattatt cacatggcta tgcagtgcga caacgattgt attggtgatc
     1921 acgttctgct tatcaggcat gttttactgg gtaaacattt ggttcatttt tgttgctttc
     1981 gcattgatga acggttccgg acgtgcgatt gggttggatc gctgggtaat tccatggcta
     2041 caacgtacac taggtaattg gtggtacggt agaccaagtt cccagtacgg caagaaataa
     2101 atagataaag aacgttattt gaatgcaaga tttcaataga aaggggctga gacaaaccgt
     2161 cacagcctcc tttttatgta aaattaaagg aataagatgc ccgagataag cctccatgag
     2221 caagacacag gtgtcacaac ctctgttaca acctcttgtt ataaacggtg ttccagaagt
     2281 agcttcttcg aaaaataagc cgaaatttca caaaaacttg aaaagtaatt ttcgtaaatt
     2341 ccttcttatt tcttgaagtt gcccacttct gtcacaacct cttgttataa acggtgttcc
     2401 acctgctgct tctcggtaat aagcccaaag aattagaaaa catggaaagc tgttttctat
     2461 tcttcgttct tattactcaa agcaagacac aggtgtcaca acctcttgtt tttatgctaa
     2521 aattgggtga gttaatctgg aaaaaggaga acaaaaatga gccttaaaga atttgctgaa
     2581 aaaagtctgc tcaaaccttg ggatgttttt attattttgc ttttgactgt tctttctttt
     2641 ttaccagtca tgatttttgg tcttcagcaa aacaccgcaa cagcagaaaa agaagcggta
     2701 ctgcgtgtgg atggcacaga aatcaaaaca ttccctttga tggctggaga agagagcaac
     2761 acatatactt acaaagatga acacggagat tataacttga tcgagataga tggcgatcga
     2821 gtacggataa aagaagcaga ttgcgatgac cagatctgcg tacgtcgagg ctgggcaacg
     2881 aaaaatggcg aaaccatcgt ctgtttgcca cataaattag tgatcgaagt ccgttcaaca
     2941 gatggaggtg acgaagacag tctgatttat tagaccttca cacaccatga gtaaattaca
     3001 aaaaatgatt tatatttcgc tacttgttgc acaaggagtg atcattggtt tattggaaaa
     3061 tatgatccct tatccttttt cttttgcgcc tggtgcaaaa ctaggcttag ccaatctaat
     3121 cacgatcgtt gccattttta ccatgaaaaa acaagacagc ttcttactac tttggctacg
     3181 actattcctg actactttgc taggcggaac gatttccaca ttcctttata gtatgagtgg
     3241 tgctttgctt agttattttg gaatgttatt ggtcaagcaa ttaggaccaa aacgtgtcag
     3301 tatcatcgga attagtgcgg ccggcggttt catgcataat gtcggacagc ttttgatggc
     3361 ttctttcatt gcacaatcat ggacagtcat gttgtacttg ccagtccttt cagttttagg
     3421 gattttatca gggattgcaa tcgggatcgc agcgaattat cttttacgac atgtgcagac
     3481 acttcaacga ttccaaaaag attatgagca gggaaccaaa caaaaatgga gcccaatgtt
     3541 taccaaaaaa taatcgtcac acaaggagtt tttttatgaa gctacatcca atgtgggaaa
     3601 actatcctgc tttaagtaaa gaattgacag aaacactaaa acggatggaa gaaactgtcc
     3661 gcctgaaaaa caaacctgtc gaacaagcca tcaaagaaac gatccatgcc ggcggaaagc
     3721 tgttgcgtcc tgcctatcag ctattgttct cacaatttgg accagagcaa gatcgagaca
     3781 aagccatcgc tttggcagct tctatcgaaa tgctccatac ggccacactg atccatgatg
     3841 atatcgttga tgaagcggat ctgcgacgtg gtcagcctaa tatccgtagt cagtttggta
     3901 atgtcgtttc tgtctatgca ggagactatt tatttgtctg ctgttttaaa ctattgtctg
     3961 attactccac ttccttgaaa agtctccaat tgaattctcg cagtatggaa aaagtattga
     4021 atggcgaatt agggcagatg gatgatcgct ataattatga actttctgta aaagaatatt
     4081 tggaaaatat ttcaggtaag acagctgaac tgttccaatt aagttgttct gttggcgctt
     4141 ttgaaagtgg aacaagcgag cgcttcgcaa aaaaagcagg agatatcgga ctttctatcg
     4201 ggatggcctt tcagattatc gatgatattt tagattatac aaaagagagc caggaaatcg
     4261 gcaaacctgt actagaagac atgcgtcaag gggtctattc tttaccgttg atctactctt
     4321 tgcaaaaaaa caaaccaaag ctactacctt atttagagaa gaaagcggca atgacagaag
     4381 aagatgtcga cgccgtacgc aaaatcgttg aacatacagg aggcgtggaa gaagctagga
     4441 agttagcggc ctcttacaca gaaaaggctt tgaaggaaat aaagaaatta cctgccacct
     4501 ctcttcgaac aaaagagaat ctcttttccc ttacccaact gattttggat cgcaaagact
     4561 gaatagggaa aggttcgata cgtaaataaa tttctcagaa ggaaatctac gtgttttttg
     4621 acaaattgaa ttgtagttct tgtatttatg ataaaaagca aaaataaaga gcaacgtccg
     4681 ttagcgacgt tgctcttttt tatcttgaat atttttttac cttttcgatt tcttataact
     4741 cataaaagtg attcttaaac cgattacaag taatataacg cctgcgagtg ttatccaatt
     4801 attcgattga cttccagttt taggaagtgc tctttttgat ttgtttccat tgtcattgct
     4861 tgttttatta gttaagttaa agttcgtcct gttatccgaa cttgtttttg agaaggagac
     4921 acttgcatga cgtgtatcgc ttgtggtaga cgacgtgtct gtactatcgc ttgaagttga
     4981 tgaatctgag ctatcacttg tggtagagga tgtatctgta ctgtcacttg aagctgacgt
     5041 gtccgtgcta tcacttgtag tggtagatga atctgagcta tcacttgtgg tagaggtagt
     5101 atctgtactg tcacttgaag ctgacgtgtc cgtgctatca cttgttgtgg tagatgtatc
     5161 cgtactgtcg cttgaag
//
LOCUS       DAZKCZ010000119         5067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000119 DAZKCZ010000000
VERSION     DAZKCZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.122"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8KN_002311"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8KN_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9189319.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="ACG8KN_002312"
     CDS             complement(481..675)
                     /locus_tag="ACG8KN_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189320.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(757..894)
                     /locus_tag="ACG8KN_002313"
                     /pseudo
     CDS             complement(757..894)
                     /locus_tag="ACG8KN_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            complement(939..1703)
                     /locus_tag="ACG8KN_002314"
     CDS             complement(939..1703)
                     /locus_tag="ACG8KN_002314"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFP9189321.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(1731..2429)
                     /locus_tag="ACG8KN_002315"
     CDS             complement(1731..2429)
                     /locus_tag="ACG8KN_002315"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFP9189322.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2441..3220)
                     /locus_tag="ACG8KN_002316"
     CDS             complement(2441..3220)
                     /locus_tag="ACG8KN_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFP9189323.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(3236..4180)
                     /locus_tag="ACG8KN_002317"
     CDS             complement(3236..4180)
                     /locus_tag="ACG8KN_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9189324.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4226..5005)
                     /locus_tag="ACG8KN_002318"
     CDS             complement(4226..5005)
                     /locus_tag="ACG8KN_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189325.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1308 a         1074 c          914 g         1771 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttaccca ataatgcttc ctacaatatc
      781 aatcattctt ttaaaaaata aatagatata tcgtttttca actttttctt tatccaacca
      841 aacttgatta ggatgtactt cactaatacg agataataaa tcttcacttt ccatactttc
      901 cctctctcct catttccttt ttatctacga ataaagaact aaaataaacc aaacttcttc
      961 ttttttacct cttcatagac tggataactg atttcatccc cgttgatcag atcctttgcg
     1021 acttgtttca tctgtactac tttttcctta ccaaagtcct tagcaatctg ctcatagcac
     1081 tcttttaagt aaaaggtacg cttagagacc ccatgggcat cagaagcagc catctgcacg
     1141 agattatgtt tgaccattgt tttggctgtt ttctgaatgt ctttgccaaa tacaccaaca
     1201 taacttggcg cagtaatttg ggccaaacat cccatatcta ggaacggaag caaatgattt
     1261 gggtcttctc gaaaatgcgc gttgcgttct ggatggacga tcactggtgt ctttcctagt
     1321 tctaacaact caaaaaaaag ccattctgca tataacggca cttccagtgt cggaaattcg
     1381 atcagaagat atgtatcatc taaatctgta aataaaattt catctcggtg gatgtcttcg
     1441 atgagcgttc cggtaatacg tacttcttgt ccttccaata acgtcaaagg caactgtctt
     1501 ttctctaatt cggcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg
     1561 tattttccat tattgtggtg tggtgtgcat aaaatatgcg tgatgccttg ctgaatcgct
     1621 ttttcagcca ttgcaatact tgcttctaag ttttctgccc cgtcatcgat tcctggtagg
     1681 atatggcaat gtagatcgat catttctctt gtctcctctc ttcttattcc ttaatttccg
     1741 tagtaataac cagcatcttt gctatgttct gctccgttgt atacaacacc taaaactcgt
     1801 gcttgtacca tctctagcag ttcttttgct ttattcaatg attctttacg tgatgtattt
     1861 tcacgtacga ccaataaagt gccatccgtt tttgaagaca tgatctgtgc atctgttacg
     1921 gctactactg gtggcatatc gaatatcacc acgtcaaata actgtcttgc ttcgtctagg
     1981 atctgatcca ttcttggcga acttaacaat tctgatgggt tgggtggctt aggtccacta
     2041 ggcaaaatcg acaagttctc gactggcgtt cgttggatta catctgctac acttcctgat
     2101 gaactaagtg ctgtacttaa tcccctggca ttgttcagct ggaatgtctt atagacgacg
     2161 ggtttacgta aatctgcatc gactagtaga actcgttgac cggatttagc aaaaacgact
     2221 gcaatgtttg ctgcagtggt ggattttcct tctccaggac ctgatgaagt cacgacaatc
     2281 gttttgattt gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat
     2341 tgttcggcga ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttgt
     2401 gctgcttttt gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc
     2461 tgcgtgatgg ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gttccttttt
     2521 tacggatacc agcgttcaat tctttcgctg acatttctgg aactgttcct aagattggga
     2581 atcctaatgc atccgtcaca tatttttcat ctttcactgt ccgatcaagt aattcgaata
     2641 agaatgccag acctacgcct accatcattc ctaatactaa gccaatcact agattcaatt
     2701 tattatttgg cgaaacaggt gtcgtattgg caactgcacc tgaaataatc gagattttat
     2761 cgacattcat cacgtctttt gcgttctctt ggaaaacttg tgaagttgtg ttagcgatct
     2821 gttgagcagt cactgcattc gtgcttattg cctggatcga gaacatttgt gaattttgtg
     2881 actgattgac ggagatcgca tctttgattt gtccagcagt cattttcaca ttgtcttctg
     2941 tttctaaacg atctttgact tcatttagta ctaaatcact gacgatcata tctttatacg
     3001 tattgatcat ttgcaggttc gtattgacat cattgacatt tgctgtgtct gattgtggca
     3061 gtgtcacgat caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga
     3121 ttcctgagat ggccaagcca gcaaacattg agatgatgat tgttaccaag tgtttgcgta
     3181 atgtttcaaa aatttcttcc aagttgattg tctcttccat tttttctcca ctttcctatt
     3241 tattctctag attcatttga tcttttaatt ctttttttac acgatcaagt tcttgatcac
     3301 tgactttttg ataggagata ttatcttgca taaagccttc tccttgcagc tgatcttgct
     3361 tgatcgtacc aaatgcctcc cgataatcta aagcgatttt tttcatttca tcaaagctca
     3421 tgtctgtttt catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt
     3481 ttaaactcaa tacttttttt gctacacctt ctacgatctt tctttgacgc tcttgtctac
     3541 catagtcacc atttggatct tcataacgca tacgagaata agataatgcc tgatccccgt
     3601 tcaatgtgat tttaccgatt gtaaagtcat agccatcttg tgagaaagtg aggtcattat
     3661 tgacttcgat accaccaact gcatcaacaa gttcctttag ccctttcata ttgatagaga
     3721 catagtgatc gattgggatg tctacatatt tttgtacggt atccatcgcc atggcagctc
     3781 cgccgaatgc gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat
     3841 acgtatcacg aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actatggcaa
     3901 ccatcatcgt atctgatctt ccttgatcga cccgacctaa atctccagta tcaatcccca
     3961 tcaatagaac agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt
     4021 gggttcgttc cactgattcg taagttttct caatagaatg tgagagatcc agatataatt
     4081 ttgctcctac tcctacaatt gctaatacta atacgataag aactgctaaa ataatcagaa
     4141 atattttttt ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac
     4201 cttgcttgaa tcaacgtttt aactgtcaat aacttatatt gctatagccc atttgctgag
     4261 caacagtttg tgcttttgtt gcgtatgctg cgcttatttt ttcattccgg atctgctcta
     4321 tttgactgag cgccgtcaaa tattgatcgt aagtagcggt ggcagtcact tgaccatcct
     4381 tcaacatcgt atcgaataac tgatctagtt gcgtgacttg cgcgatctga gtatcagcga
     4441 atcctaaata ttgcataaca accgttttcc aaggactatc tttaaaaaag ctcatatttt
     4501 cacgtacacg agcgacatct gcacttgtca ccttttcttt gatgatgaca tcgtctacag
     4561 cttgctgcca gtttgataca tttttagtaa acaaatcgtt gacagctgtc tggatcttca
     4621 gttttgattc tgcactatct aactgtgtca cgattgcttt cttctcatct tggattgcaa
     4681 cagcttcttt aggcaattct gattctttga tgtcgtaatc atctgctgaa agtttgacat
     4741 aggatagctt atttgacaaa cttgtgattg tttcttctgt tatttcaggg tttagaaaaa
     4801 ttttttcttt tgtactatac agcttgtcta cttcttttga taaagaagtc aaggtgtctt
     4861 tttgattggt gatggcagat tctgcataat tgatccgttg ctgatgttgc cgctgattat
     4921 tgatatggta accaaacaaa cagatcgcaa tagcacctgc acctaccgcc acagcagtca
     4981 atgacttgac gaatggcttt tccatagatt cactcctaag tattgatgat agataaatta
     5041 agatataatt cacttacata aaaatga
//
LOCUS       DAZKCZ010000120         5059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000120 DAZKCZ010000000
VERSION     DAZKCZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.123"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG8KN_002319"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG8KN_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFP9189326.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG8KN_002320"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG8KN_002320"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9189327.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACG8KN_002321"
     CDS             complement(2169..2906)
                     /locus_tag="ACG8KN_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9189328.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACG8KN_002322"
     CDS             complement(2997..3524)
                     /locus_tag="ACG8KN_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189329.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACG8KN_002323"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACG8KN_002323"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9189330.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACG8KN_002324"
     CDS             complement(4401..4886)
                     /locus_tag="ACG8KN_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9189331.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1342 a         1128 c          864 g         1725 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttc
//
LOCUS       DAZKCZ010000121         4967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000121 DAZKCZ010000000
VERSION     DAZKCZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.124"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..1180)
                     /gene="nagA"
                     /locus_tag="ACG8KN_002325"
     CDS             complement(32..1180)
                     /gene="nagA"
                     /locus_tag="ACG8KN_002325"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9189332.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1256..2092)
                     /locus_tag="ACG8KN_002326"
     CDS             complement(1256..2092)
                     /locus_tag="ACG8KN_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189333.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSQDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2276..3493)
                     /locus_tag="ACG8KN_002327"
     CDS             complement(2276..3493)
                     /locus_tag="ACG8KN_002327"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9189334.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     ITSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3642..3803)
                     /locus_tag="ACG8KN_002328"
     CDS             complement(3642..3803)
                     /locus_tag="ACG8KN_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189335.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3876..3959
                     /locus_tag="ACG8KN_002329"
     tRNA            3876..3959
                     /locus_tag="ACG8KN_002329"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3910..3912,aa:Leu,seq:gag)
     gene            complement(4199..>4967)
                     /locus_tag="ACG8KN_002330"
     CDS             complement(4199..>4967)
                     /locus_tag="ACG8KN_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189336.1"
                     /translation="YNYKLSVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFN
                     FLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLII
                     YFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLC
                     LIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVL
                     MAVKELMKSMSLQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1523 a         1020 c          799 g         1625 t
ORIGIN      
        1 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
       61 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
      121 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
      181 tcatgactgc ttgttctggt gttgctaatc cccaatctac aacattttta attgcttctt
      241 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
      301 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
      361 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
      421 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
      481 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
      541 caaatacact ggcgccagct tcaacagctt tttgcgcttc ttctagcgta gcgttactgt
      601 gacctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
      661 gacgttccgg tgctaaagca atttttttga ttaaaccgcc tgaagcttct tgccattcat
      721 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
      781 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
      841 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
      901 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
      961 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
     1021 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
     1081 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
     1141 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
     1201 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
     1261 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
     1321 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
     1381 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
     1441 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
     1501 ttgatcgttc gtcagctgat tggtgcaaca gttgaatgct tcgactcaaa taatagcgtg
     1561 cttcagccgt ttttcctgca acaccatata tttttccaat gcaactgatc agttgtattt
     1621 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
     1681 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
     1741 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
     1801 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
     1861 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
     1921 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
     1981 gacttaatac actttgtgaa caaatatctt gcgaaagcat tttttgcgtc attttttgtt
     2041 cttttcgaat ctctttaatg atcgtgccaa aggactcgat taacgtttcc ataagtatcc
     2101 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
     2161 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
     2221 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
     2281 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
     2341 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
     2401 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
     2461 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
     2521 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
     2581 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
     2641 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
     2701 tatagccttt aacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
     2761 ctacacgatg agatttggat agaccattca gcgtaacaac gaacaaatca ggcgctaatg
     2821 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
     2881 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
     2941 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
     3001 tttttgatgt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
     3061 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
     3121 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
     3181 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
     3241 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
     3301 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
     3361 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
     3421 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
     3481 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
     3541 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
     3601 aaaacgtaag tattaaaaaa acacaaacaa gcgttaagaa cttaattgtc atgatagtca
     3661 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
     3721 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
     3781 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
     3841 gtaaaacctc gctatcatta ttgtgttatt attacgcaga tgtgttggaa ttggcagaca
     3901 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
     3961 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
     4021 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
     4081 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
     4141 taatcgaaaa acgagtaaaa tccctcacct aatactagag cgagggattt tttgtatatt
     4201 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
     4261 taaattcatt tttttgtaaa ctcatgcttt tcattagctc tttaactgcc attagtacta
     4321 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
     4381 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
     4441 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
     4501 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
     4561 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
     4621 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
     4681 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
     4741 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
     4801 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
     4861 atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta
     4921 attctgctaa gcttattatt gaaaatactg ataacttata attatat
//
LOCUS       DAZKCZ010000122         4932 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000122 DAZKCZ010000000
VERSION     DAZKCZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.125"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8KN_002331"
     CDS             85..972
                     /locus_tag="ACG8KN_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9189337.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8KN_002332"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8KN_002332"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9189338.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8KN_002333"
     CDS             2274..2720
                     /locus_tag="ACG8KN_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9189339.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG8KN_002334"
     CDS             2993..4345
                     /locus_tag="ACG8KN_002334"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFP9189340.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG8KN_002335"
     CDS             4516..4803
                     /locus_tag="ACG8KN_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9189341.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1394 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tt
//
LOCUS       DAZKCZ010000123         4731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000123 DAZKCZ010000000
VERSION     DAZKCZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.126"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8KN_002336"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8KN_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFP9189342.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACG8KN_002337"
     CDS             complement(2064..3062)
                     /locus_tag="ACG8KN_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFP9189343.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACG8KN_002338"
     CDS             complement(3288..3941)
                     /locus_tag="ACG8KN_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFP9189344.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8KN_002339"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8KN_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFP9189345.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1388 a          995 c          742 g         1606 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat c
//
LOCUS       DAZKCZ010000124         4718 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000124 DAZKCZ010000000
VERSION     DAZKCZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.127"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..1353)
                     /gene="dltD"
                     /locus_tag="ACG8KN_002340"
     CDS             complement(85..1353)
                     /gene="dltD"
                     /locus_tag="ACG8KN_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFP9189346.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            complement(1356..1589)
                     /gene="dltC"
                     /locus_tag="ACG8KN_002341"
     CDS             complement(1356..1589)
                     /gene="dltC"
                     /locus_tag="ACG8KN_002341"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFP9189347.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            complement(1634..2839)
                     /gene="dltB"
                     /locus_tag="ACG8KN_002342"
     CDS             complement(1634..2839)
                     /gene="dltB"
                     /locus_tag="ACG8KN_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFP9189348.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            complement(2836..4350)
                     /gene="dltA"
                     /locus_tag="ACG8KN_002343"
     CDS             complement(2836..4350)
                     /gene="dltA"
                     /locus_tag="ACG8KN_002343"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFP9189349.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            complement(4361..4510)
                     /locus_tag="ACG8KN_002344"
     CDS             complement(4361..4510)
                     /locus_tag="ACG8KN_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFP9189350.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
BASE COUNT         1484 a          945 c          781 g         1508 t
ORIGIN      
        1 gttcaggcaa acttggtatt tttatctttt attcagaata ttcaaaaata aagaaaacaa
       61 gcgagcaaca ctcgcctgtt ttctttattt taattgtagt ttatctggta tttgatgttg
      121 ccaagatttg ctaaaaaaag catcgtcaag atgatactta ctagcagtaa tatgattttc
      181 ttctaaaaac ggtctgatct gttgatctgc agccaaccag cctttccagc ctagatggat
      241 cgtgtcttcc ataaaatagt tggtacccgc ttgattgaca aaatcagcta ttcgattgaa
      301 tccttggcta tttaattgaa atttgatctt tttggcaaat ccttgtaaca tttcttgaga
      361 tagtccggta taatcgctcc atttctcgtt aaccggtggg atgatgaaca atacttcgtt
      421 atggtttttt gctaattgat ccaagaccag ctgaaaatca gaaaattctg gcgaaaaacg
      481 ataatcccaa tttttttgtg actgtttgag ctctggctcg tgggctcgga tacgatgcgt
      541 ataaaaatcg tttttcaacc cgaatggatt attcgtcgtt tctcgttctc caatttgatt
      601 tgctaaaatg gataattctg tttcttggta ttgttttggt aatcgttttg actcatgatc
      661 gattttttgc tgtcgatctt tcaacccaat attgctgaat acttcatcct ctctcttcag
      721 catgttccat tggctgcgca gctgattcaa agattcaggt aaaggtagct tccctttttt
      781 gatcgtttgc agaatcgccg tcaacgtatc attctctttt acttttgaga aagttaataa
      841 tcttctagct aaatagcgat cagcaggcgt tactttcttc attgaaaaca accagtcgta
      901 tgtttgaagt tcagaataat acgtattaaa gtaatcagtt tttacaccat ttttaacgaa
      961 ccactgagga gaaataataa agacgacttt cttgtttttc atagcatctc ctgcagaacg
     1021 catcatcata tattggctta acgattgtgt cccgggtgct cccagcaaaa atggacgata
     1081 gtttcgctgg tatttttctg ccagaacaga cggatgaaat ggactgattc tgcttaattc
     1141 acttgagcca aaaaaaggaa tatattgttt ttcagataat gcataatttt ttatcgcatc
     1201 tcctttcaac acgttcactg acatggaaga agaggcacgt ctaatcgttt ctggattaga
     1261 gatatctact tgataggagg aggtaaaaaa cacaaacagt aagccaacag ctaaaacaac
     1321 cgggccgaaa atagcaaaaa gtttcttttt catactcaac ccttcaatgc agttgcttgt
     1381 tgaatgattt tattcggtgt gctccaatcc tctcgctcga attcagatac agggacttgg
     1441 atatccagct ctccgtctag ctcgactaat aattgaacag atgctaaaga atcaagcaac
     1501 ccttcatcaa atagatcaat atcacgattt tttcttactt catctgttcc tgttatttct
     1561 tctaagattg ataaaatttg ttcttccatt ttccttttct cctttgttat ataaattaat
     1621 atgtcactat tcgttagcta aaaaaaagtt tatctaaaaa tcctgaaaaa atcaggaagc
     1681 taaaacatac tgcttgaaat gtcaaaaata cggctaagcc atgcgtaaat ttgttagaag
     1741 gcaactgctc tttatgcttc ttcttaaatc ggatccatgc atctgtcaga cagatcagac
     1801 aagcatgata cagtccatat acgatgtagt accacgtcaa tccatgccat atacccatta
     1861 gaagaaacaa agcaaaataa ccgatatttg acattacgat cttgctttta aaaaattttt
     1921 tcttcatcat gaagaacatc aatcgcatat atacataatc acggaaccaa aaggacagcg
     1981 tcatatgcca acgattccag aattctttga tattccatga taaaaaaggt ttattgaaat
     2041 tgattggtgt ctgatatccc atcatgtagc ttgtaccgac tgcaaataag ctatagccag
     2101 caaaatcaaa aaacaaatac atactgtaca cgtacatata acctactaat gcccatgaaa
     2161 taccaccatg ctgtaaggca atgcgtccca ctacaggaag cagtacttga ccaaaataat
     2221 aaccgataat gaatttatat aaaaaaccga caaaaatatt ttggacgcct tttcccaaaa
     2281 gatcaacata tttatcacga acaggcggat ggagctgatc tttttcaaat ctcctatagc
     2341 gatcgattgg tccagaagag attgttggaa agaacagcag aaactggata taacgaaatg
     2401 gcttatatgt tttgatcatg ccatctctta tttccatgat catttgtaca gatttaaagg
     2461 ttaaataaga gattcctaaa aaaccaagta acgatgcttt tccatgttca aaaaaaggag
     2521 tcactttcgt taaaaacagt ggaattatcg ctaaaataac ggcggcaaaa aaccatcctg
     2581 tctgattttt cctttgccga taaacaaaat agccccatac aagtatcgtc tgccacaaaa
     2641 catacccaat cagcgccgct ccctgttgcc aataattacc tccaaagctg acaaataaaa
     2701 agaaaagtgt cactgctgat tgataaactg gtaatctttt ttcccatatc aataaagata
     2761 gaacgatcgg tacaagcgca atggctaaaa gaaaaaaata aaacggctct gcataaggga
     2821 tcatatgagg taacatcatt caggattcac ctcagctgtt aatttttttc gatcgatttt
     2881 tccattaact gtctgaggca gcagatcgac ataatggaac ttctgaggaa tcatataatc
     2941 cattacaact tctagtaatt gttctttgat agatttagtc aactgaaaat ccggctgttt
     3001 cgttttgttt tctaatacga caaaagcaac cagctgctgt acttttgttc cttggtattt
     3061 tggtacgacg acagcttgtt tgattcggtt gtcttggagc agataatgtt cgatatcccc
     3121 gagttcaatc cggtatccat gtaatttgac ctgctgatcc atacgtcctt cataaaataa
     3181 aagcccatat tcatctagtc tacctgcatc ccctgtatgg taacttgcta agtcattttg
     3241 acaaaagaac gcagcagctg tcttttcggg attgttcagg taaccttttg atacgcttgg
     3301 tcctgcaata atgatttcac ccgtttctcc ttttgaaaca ggtttacctt cttggacaat
     3361 agaaactttc gtgtcttttt ttacgtagcc aatcggtagt cggtcataac tatctagtac
     3421 atgctggtca atctgaatac ttgaaatagc tacagttgct tctgttggac cgtaagtatt
     3481 atatatccgt gcatttggga atcgatcaag tagactttct gcagtttttt tcgtcaattc
     3541 ttctccgcaa aataaaaaca tgtccaattc cggcacattt tctccattga atgtaggttc
     3601 catcaagcaa atatccataa atgatggagt agaaacccac acattcaact tcaaggctgg
     3661 caatgtcgcg aataattgac gaaagtcttg aacaactgct ttatttaacg gcttcagcat
     3721 tccgccagaa actaatgcag gataaataga aaataccgat aaatcaaaag aaaatggcgc
     3781 ttgggctaag aaatgcatac caggcttgat cccaaagtct tccatcgtcc attgaacaaa
     3841 gctgactaga ttatcatgac tgatttgtac gccttttggt tctccagtag taccagaagt
     3901 aaaaataata taaaaatttt ctgtaccttt tacgctattt ctgggataaa atcctactgg
     3961 tatcttcttg atttcttgaa attctttttt tgttatgaca ggaaccgaag gaatatcttc
     4021 tatccagtcc ccgatactga tgaccataga tggtttagcc acccgaaaga tcgataggat
     4081 acgctccttg ggtgtatgct cttcaatcgg caaataggca tggccagatt tgacaacccc
     4141 tagaaatgag acaatcattt caaactccaa attgccaaaa acgacaattg gtccatcgcc
     4201 ttctaccttt ttttctaaat aataagcaag tgcgtctgat gcttgtttca attcaccaaa
     4261 agtatgcgtc tcatccgcct gataagcaac tcgatcaggt tcatttattc cccaatcatc
     4321 gattgcttca ataatcgttt tgatttccat actcttcctc ctaaaattcg ttatagataa
     4381 aagttcctcc ttgaatattt ttatagtgat acagataaat caggaacagc agtactgcac
     4441 tataaaaaat tgttcgaatg atgaattttc cccaataatt caattttgga ttagaacgcc
     4501 atttgttcat tggattccct cctcatataa cctcacacaa agtgtaacta attttgacaa
     4561 aaagaaacat gtgtctagct tacagaaacc gtttttttac taacagtttt gtcacatagc
     4621 ttttataaaa atagcacatc aaatcactgc ttttttcttt ataatttctc gtacttttaa
     4681 aaaatataaa aaataaagag gttgtgacaa aaatcgtg
//
LOCUS       DAZKCZ010000125         4675 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000125 DAZKCZ010000000
VERSION     DAZKCZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.128"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..1083)
                     /locus_tag="ACG8KN_002345"
     CDS             complement(121..1083)
                     /locus_tag="ACG8KN_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9189351.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1085..2524)
                     /locus_tag="ACG8KN_002346"
     CDS             complement(1085..2524)
                     /locus_tag="ACG8KN_002346"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9189352.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNKNWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2709..4655
                     /locus_tag="ACG8KN_002347"
     CDS             2709..4655
                     /locus_tag="ACG8KN_002347"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFP9189353.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1481 a          800 c          773 g         1621 t
ORIGIN      
        1 aaagtctaac ttttgggggt cactacaagt tgacaactcc tttttgttta caaatcaatt
       61 ggatatctac aaattgtatt ttctaacgaa ttttatagac attaaaaaat taatttaacg
      121 ttaaatactt cctccagaaa gtattgagat tggtaaaata taatccttat ttgtcttttc
      181 gcctttaatt tttttcagaa gaatatcaac aataagactt gcaatttcat caattggttg
      241 tcttattgtt gtcaattgtg ggaaaaattt ctcaataaaa gaggtaccat cgtatccaat
      301 aatttttaaa tcttctggga tattaagttt aagttgatga gcaattttca tagtgagaat
      361 ggctgttaaa tcatcagaaa caaaaatacc atctggtttg tgatttataa tagttgactt
      421 tatttccatt tcacgacgga tagtagacag attattagga attttgaaaa tttttccatt
      481 tggattttga aaagaaaaac ctagtgcacg taattctgtg ggagaatccg tgttatcatt
      541 tcctgtaatc ataatggtat tttgacaacc atttttttgt aaagtcttag ctgcaagttt
      601 tcccccttca aagttatctg aagaaacaat ggaaacatta ggagctaaat ttctatcaaa
      661 agcaacaata ggtgctctca cacgctcata atcagcaatt ccaagattat ggcttgatga
      721 gattatgcca tcaacttggt tggcttcaag catttcaatg tattctctct ctttagcagg
      781 atcattttga ctattgcaaa taatcgtttt atatccatgt ttaaataact ctatttcgag
      841 atactcaatg agttcagagt aaaagatatt actaatatta ggaaaaataa gaccaataag
      901 ctgaggtgac tttccttgaa gtccacgtgc taaattattt ggtttataac caagagtttt
      961 catagcttca tgaacttttt gttttgtttt ttctgataaa tatcccttat tattaatgac
     1021 gcgtgaaacg gttgttgggc taactccagc taattcagcg acatcagtta attttgctac
     1081 cattttaata ttttaattca aagtattttc cagtaggttc gcctgatttg atttgaatac
     1141 cattttggtg attttcaggg aatacacgac tagtaaacac tttttctcct ttattaataa
     1201 aaatctcaat gattgagtta tctacaaaaa tgttagcagt tgtttcttta gtatcaattg
     1261 agcattcacg aatcgtacca aagtcagtgg catattgaat gcctgctttg ctgcgatcta
     1321 aaataatttt tccttctttg gtatctacaa tcaagctaag tccatttcct ttactatcag
     1381 agaaaagaag taactccgat ttctgatttg gaggaaaagt tagttcaagc tcataagtat
     1441 tatttgtttc tgcttttgct gtaaattgtt cagaactagc acgtaatgat gtaatagctt
     1501 ctactggata ttggtaaagt ttaccatctt ttattgaaag ttcttttaca agactcattg
     1561 ctccctgata atcatactta tcagttggat aatcgatatc aggtagtcca atccaactga
     1621 caatcagagc acgaccatca ggagtattga atccttgagt cgcatacgct tcaaatccat
     1681 aatcaagatt ttgaatttct gaagcgccca caagttttac tttttctatg tcaagcgatt
     1741 gacagatctt aaaagtattc ggatagatat tcccataatt tagctcagat ttatccaacc
     1801 cttgaggaca ataaatgaga acaggttttt tgtctacaaa tactagattt ggacactcaa
     1861 tcatgtattc tgaaccagtg ccgccaaaat caaggttgcc aacttcctcc catgtttcca
     1921 cgttattgtc atccgctttg taaagtttga taaagcctga tttttctaaa ttttgtgctc
     1981 caacaatcgc atacaattgt ccttcataat taaacatttg tggatcacga aaatgatcag
     2041 ttacatcagc aggtttatga ataagaacat tctcaccttt cgtaatgttg tagtctttat
     2101 ccatccatgc tccgatttga agtgtatcac gttcccaatt tttatttcga acgtttcctg
     2161 tatagagtaa gaatagattc ccctctattt catatgcaga accggaataa gcgccgtggc
     2221 tatctcgttt tgtgtctgga agaagttctg ctcctgttac atgaaaatga actaaatcat
     2281 ctgattcatt gtgtacccat tctttcaacc catgagcagc tccaaatggc caattttgat
     2341 aaaaaagcgt atattttccg ttaaaaaaag aaaaaccatt aggatcgttt aacaatcctg
     2401 ttttaggttc gatatgatag ctagcacgcc aaggtgatcg ttttacatta tccttaattt
     2461 tatccatctc atctttagtc caatcagaat aagcacgata tcgtatttct cgaggtaagt
     2521 tcatatttta ctccttcaat ttatcttttt acaactagta tggtaaacgt tttccgttga
     2581 aaaatcaatc atttcattta aaaaaataaa ctatatgaca aacgtttgac atatttttaa
     2641 gatatgctaa aataaatttg catttcagtg aaccgttttc acgtaaaaat aaaaaaggag
     2701 tgattcgaat gaattacagt aaagtagctt ccgacgtaat acaagcggtc ggaaaagata
     2761 accttattgc agctgcacac tgcgctacac gtcttcgtct cgtactaaaa gatgacacga
     2821 aagtgaatca aaaagcttta gacgaaaatc cagatgtgaa aagcacattt aaaattgatg
     2881 gacaatatca agtgattatc ggtgcaggtg atgttaattt cgtttatgac gaacttatca
     2941 aaaaaactgg actttcggaa atttcaacag atgatttaaa acaaattgct agtaaagata
     3001 aaaaattcaa ccccattatg gcattaatta aattattatc agatattttt gttccaatta
     3061 tacctgccct tgttgcaggt ggtttattaa tggcattaca aaacttttta acagctgctg
     3121 gactttttgg accaaaatca attgaagaaa tgtatcctgc gattgaaggt atttcctcaa
     3181 tgatccaact gatgtccgct gcaccattca tgttcttacc aattttagta ggtatttcag
     3241 ctgctaaacg ttttggagca aaccaatttc ttggtgctgc aattggtatg atcatgacaa
     3301 caccagatct tggtggctca actgaatatt ggaatatttt tggttatcat gtttcacaaa
     3361 caaactatgc ttatcaagtt attccagttt tagcagctgt ttggatatta tctatattag
     3421 aaaaattctt tcataaaaaa ttgccatcat ctattgactt tacattcacg ccattattat
     3481 cagtaatgat tactggattt ttgactttca cagttattgg ccctgtaatg ttattgttgt
     3541 ctaatggaat tacagacatt attgtttggc tatataacac tactggattt attggaatgg
     3601 gtatttttgg aggaacatat tcacttatag tcatgactgg tttacatcaa tcatttccag
     3661 ctattgaaac tcaattactt tcagcgtgga caaatggtat cggacatgga gactttatct
     3721 ttgtagttgc atctatggca aacgttgctc aaggtgctgc aacatttgcc atttggttct
     3781 taactaaaaa ttctaagaca aaaagtcttg cttcctctgc gggtgtctca gcacttcttg
     3841 gaattacaga gcctgccttg ttcggagtta acttaaaata ccgctttcca ttcttttgtg
     3901 ctttaattgg ttcaggaatt gctacagcag tagcaggatt acttaaagta gttgctgttt
     3961 cacttggttc agcaggtttc ttgggatttc tttcaattaa tgcaacatct attccatttt
     4021 acgtattgtg tgaattgatt agctttgtag ttgcttttgc aattacttac ttctacggta
     4081 aaacaaaagc tgctggtatc tttaaagcag aagaaattgc tgaacaaaca ccagttaatt
     4141 cttcaaaagt agcaacaaca gatccaatat ctactgatgt tgaatcaaca caaacaaaag
     4201 tttcatcaga aacaattgtt agtcccattt ctggtaaagt gattagtctt tcttcagtaa
     4261 atgatccagt cttttcatca gaagctatgg gaaaagggat tgccattaaa ccaaatggaa
     4321 acacggtcca ttctccagta gatggtacta ttcaactcac gtttgaaact ggacatgctt
     4381 atggtattaa atcaaataat ggtgctgaag ttctaattca tattggtatt gatactgtat
     4441 caatgggagg aaaaggattt acacaaaaag ttactactaa tcaaaaagtt agaaaaggcg
     4501 atattcttgg agcatttgac cgttcaatga ttgagaaaga aggtcttgat gatacaacaa
     4561 tgattattgt gaccaatact tcggattatt cagaagtaaa tatagtagct gaaaatacag
     4621 tcactgaggg tactgattta cttgaaatca aataaatata tttccctgag taaag
//
LOCUS       DAZKCZ010000126         4607 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000126 DAZKCZ010000000
VERSION     DAZKCZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.129"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..396
                     /locus_tag="ACG8KN_002348"
     CDS             <1..396
                     /locus_tag="ACG8KN_002348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9189354.1"
                     /translation="SIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRTFRHCLF
                     CKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYLVAPSCF
                     ISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            553..810
                     /locus_tag="ACG8KN_002349"
     CDS             553..810
                     /locus_tag="ACG8KN_002349"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9189355.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            897..2135
                     /gene="istA"
                     /locus_tag="ACG8KN_002350"
     CDS             897..2135
                     /gene="istA"
                     /locus_tag="ACG8KN_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFP9189356.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            2125..2865
                     /gene="istB"
                     /locus_tag="ACG8KN_002351"
     CDS             2125..2865
                     /gene="istB"
                     /locus_tag="ACG8KN_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFP9189357.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            3284..3439
                     /locus_tag="ACG8KN_002352"
                     /pseudo
     CDS             3284..3439
                     /locus_tag="ACG8KN_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3506..>4607)
                     /locus_tag="ACG8KN_002353"
     CDS             complement(3506..>4607)
                     /locus_tag="ACG8KN_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9189358.1"
                     /translation="YKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSV
                     LLIILFVSTKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFI
                     ILTPQLFMQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISK
                     AQAEGKSENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLIS
                     CLIIVVSPIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVL
                     VVVELLVFFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDM
                     AIKSIMIFIIYLPFVYFLFPDIRNEVRKIIRK"
BASE COUNT         1568 a          885 c          823 g         1331 t
ORIGIN      
        1 tctatcaaag agctcttcac tattaaagat aaaaatattg aaattaattc ttttcaagaa
       61 actttttata aggacaagaa agccatcgta atcgaaggtc cattagttcg cacttttaga
      121 cattgtctat tttgtaaaga atctgcaaag cagatcgaga aaaacggcaa gaagatttca
      181 atgattatct taaatcgcag tgggaataaa caaacctatt tgcgtcttag aaaacaacat
      241 taccactgcc aatcatgcgg gaaatatttt actgctcata cgtatcttgt cgctccttca
      301 tgttttattt cgaaacaagt tcattacacg atattagaag agttgaccga gcgtcaagca
      361 ttaaagacaa ttgcgaaacg ttgtgatttt cagtaacgac agttcaacgg acattatctg
      421 ctctatcggc aacgctgcga cagaagctag actatttacc tcattgttta ctattcgacg
      481 agtttcgctc tctttcaatt ttccatggga agttctcctt ttcttgtatg aattgagaga
      541 cagggaagct ctttgacatt ttgtccagcc ggagaaaaaa aagatttgat tgcttatttc
      601 atgcgtttta caagaaaagc tcatatgggg gttcgataca ttgtcacaga tatgaatgcc
      661 ccttattttt cattagtcaa agaacccttc ccaaatgcac aaatcatcat tgattgcttt
      721 cacattgggc aacacttgaa caagtttttc gatcgtgttc gaaaacgtgt gatgaagcag
      781 ttgaatcaaa aatgtcaacg gcacctttaa aatgtaggaa aacggcaatt taaaattgtt
      841 ggtttaccta catttttgta tgctcttctt atcccaaatg aaatggaggc tcagggatga
      901 gaaaagacat acgcgaagga gtgatgatct acgtgatcaa tgaaatcaaa cccaattatg
      961 ctgcactagc aaaacaatac gactgcgact accgaacagt taagcatgct tacgaagaag
     1021 cccaagttaa agaatcaaaa cctccagaac gtaagaagcg tccaagtaag ttagatccct
     1081 acagagaaat catccaagac aaaatcaaag accaatgcag tgcttacagc attttcaagt
     1141 ttattgagca caaaggattt ggaggaagct acagtttagt aaagacctat tgtcgccaga
     1201 ttaagcaggc gaaagtacac aaggcaaccg tccgagtcga acatagccct gggctttctg
     1261 ctcaggttga ctggaaggaa gatatgcgac taatcagtaa tcagggcgaa atttttgagt
     1321 tcaatatctt tttgtacgtc ttgccatatt ccaagaaaaa atatgtcaca ctaactttcg
     1381 accgaaaaca agacacactc tttgagtgct tagatgatgc gttttatcaa accggtggcg
     1441 ttcccaaaga aatctggttc gataatatga agacagtcgt tgaccattct cgtagtcagt
     1501 tttccaaacc gcatttcaac aaacgttttt atgctttcag taaggatgcc ggcttcacac
     1561 caattgcctg tcggccattt cgtccgcaaa caaagggctg tgtcgaagct ttagccagaa
     1621 ctgttgaccg cctacgggtc tataatcatg aattttatga tgccgttgag ctgtgtcatt
     1681 tagtgaataa cctttgtgtt gagttaaata acgaggtttc tcaatcaact gatcaaacac
     1741 cagatgaacg ttggcagacg aacgaaaaag agcacctgca tccataccaa aaaaaccttt
     1801 tgaatcccta ttttgaagac tttattcgcc gtaaagtcac caaggaagaa atggtcattt
     1861 tccgtaaatg caggtactct gtggcgcctc gctatatcgg taaagaagtc gaaatcgaac
     1921 tctccgataa agaagaccaa gtacaaatct attataacgg agaactgatt cggtcgcatc
     1981 cattaacgac aaaacagctg aactaccacg aggaagacta tttccaaatc ttgaaatccg
     2041 atgtcatgag tcacaaagaa gacgatggaa tcagagcgta tactcgtgaa agtctcaagc
     2101 aatatgatct ggtggaggtt gccgatgaag aatagccatt accaagaact gatgaccaac
     2161 ttagagactc ttggtttgaa tcatatgcgg gaatatgttc ctaactacat cgaagtggcc
     2221 aatcgagagg agctttcttt aacggaagct cttctcgagc tgacacgcag agaagttgat
     2281 catcaaaata agcaaaaaat gaatcgtgtg gtggcacgcg cccgcttttc caagagaacc
     2341 agcctggaag agtttgattt tgccttccag ccaagtatca ataagcgcga agtgctagac
     2401 ttaaggcaca tgggctttgt ggaaaaacat gagaacttgg tttttatcgg cagtcctggt
     2461 gtaggcaaga cacatctgac gattgcattg ggaatcgaag cctgtcgtca aggctatcgg
     2521 acactattca tcacctgtca tgagctgctt ttgcgtctgc gaacagccta tgaaaaaggt
     2581 accctagagc gtgttctcaa acgatacgct cgctatgacg tcttgattat tgatgagatt
     2641 ggctatcttc cgattcaaaa acaggaagct gatttattct ttcagcttct tgccatgcgc
     2701 tatgaacacc gctctacgat cattacgacg aacattgtct tatctcgttg gggggagttg
     2761 ttccaaagtt ctgagatcgc agctgccatc cttgaccgtt tagtacatca cgtcaaggtg
     2821 ttcaagatca caggcaagtc ttatcgtatg agagacaaga tctaaagtgt cgattgtcga
     2881 caattactat tgccgaaaac ctacattttt atcctgccca aaacctacaa ttttaagctg
     2941 cccttgacat caaaaagaca cttcacaagc taagtgctat cgacaattga aacctttgta
     3001 taaattactt ctataatcag atgacgagtt ggattataca acattcaaga aatggcagaa
     3061 ttttcaatgg cgctatttaa cagaaagtga agtagttgat tgtctaccct caatctctta
     3121 tgaacttaaa atggcttagc agtattatca aaagctgctt aacaattatc acaagaaaca
     3181 gctagctact tcgagaaatg accaaagaag ttccctcgaa acttcactat cttaaaaagc
     3241 ctttttcata tctgaaaaag gcttttaact agcattttcg aaaaagtatt ttaatacaaa
     3301 attagagaat cttcgcacac atatcaaaac attgaaacga tttgcttatg gattttgcag
     3361 ttttaccaat atgcgcttgt ggatttttct aatcaacggg ttaataagct atgcattaaa
     3421 agctagtaaa tcttactaac tttgttgatc ataaatgacc ttatcagccc gatttgacaa
     3481 agaactactt ttttacaaca ttattttatt ttcgaataat ctttcttact tcatttcgta
     3541 tatctggaaa aagaaaataa acaaacggaa gataaataat gaaaatcatt attgatttaa
     3601 ttgccatatc taaaaatgaa gaagagaata attgtggaag aaagtaatag gccgaagtag
     3661 tcaaagtaag attgactata aaaaataaga caacgttctt cccaaagaaa taggatattt
     3721 ttatttcttt tcgtacaaaa aatactctaa agaaaaagac taataattct actaccacga
     3781 gtacgattgt agccgctaca gcgccataag taggcacaaa agctacattt ccaataatac
     3841 ttacaatcgc accaatgatc attggataag catactcctt atctttttct atagcaattg
     3901 caaattggtt agcaaaaact ccaccaattg gactaacaac tataattaga caagaaataa
     3961 gcatgtttat tgtcatcggt ttgaaattct ccccaaaaaa ccatggaaca aattcttttg
     4021 tattgataaa caccacacta aaaagaatta atgcaataat aatcgtgtaa tcaaaagatt
     4081 tttttgtgta aactaaaata ttttctgatt ttccctctgc ctgtgcttta gatattttag
     4141 gcaacataac aatagtaatt gatgttaaga ctgccaatat aatacgagca attttttgag
     4201 actgatcata ataagataat gctgttggat ccacaaaata cccaactatc gttttgtcta
     4261 aagttgtgta cacttgcata aacaactgag gagtcaaaat aataaaagct ggtttccaca
     4321 actgtttaaa aacattagag ttaaagagtg gtttacctac ttttccgtgg atactcaacc
     4381 agaaaactaa acttcctaaa aagttagtaa ttgaagtaat gaacacataa atccacaaat
     4441 catctttagt tgaaacaaaa aggattatta acaataccga tgataatttg ataactgtat
     4501 ttctaataac cactttcgaa aattcttcaa ttccttgaaa aaaccaagaa atatctaatg
     4561 ttagccctgc aataaatatc aattgaatta agtataaatt tttatac
//
LOCUS       DAZKCZ010000127         4485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000127 DAZKCZ010000000
VERSION     DAZKCZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.130"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..625)
                     /locus_tag="ACG8KN_002354"
     CDS             complement(77..625)
                     /locus_tag="ACG8KN_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189359.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(626..1483)
                     /locus_tag="ACG8KN_002355"
     CDS             complement(626..1483)
                     /locus_tag="ACG8KN_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9189360.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(1769..2056)
                     /locus_tag="ACG8KN_002356"
     CDS             complement(1769..2056)
                     /locus_tag="ACG8KN_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HFP9189361.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKTLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(2046..2375)
                     /locus_tag="ACG8KN_002357"
     CDS             complement(2046..2375)
                     /locus_tag="ACG8KN_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9189362.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2452..2724)
                     /locus_tag="ACG8KN_002358"
     CDS             complement(2452..2724)
                     /locus_tag="ACG8KN_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9189363.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(2791..3471)
                     /locus_tag="ACG8KN_002359"
                     /pseudo
     CDS             complement(2791..3471)
                     /locus_tag="ACG8KN_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(3614..3799)
                     /locus_tag="ACG8KN_002360"
     CDS             complement(3614..3799)
                     /locus_tag="ACG8KN_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189364.1"
                     /translation="MDDVLTDIQNLGYEIILVSFGAIVIGANKLNFHKYPETLKIKWL
                     AQLSLHNKNTFEIISKK"
     gene            complement(3892..4434)
                     /locus_tag="ACG8KN_002361"
     CDS             complement(3892..4434)
                     /locus_tag="ACG8KN_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFP9189365.1"
                     /translation="MINVSEIKTNKPQKYETLLQEKVYAALESLDISYERVETDEVIT
                     MEDCEQVNLKLDMHMVKTLFLCNRQKTNYYLFITSGDKPFKSKDFSKALGISRVSFAS
                     KEDMTSMLQTKIGAATVFSTLIDTKNKIKIIFDKDVTDDRWYGCSDGTTTGYLKISTN
                     EIIAKLLPYTKHQMEIVKIS"
BASE COUNT         1304 a          855 c          632 g         1694 t
ORIGIN      
        1 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
       61 cgttcttctt tgattattat ctaactttat tattcattcc ccaccctttg cgcagtgagt
      121 cttcggtatc ttgttttatc tgactggtag ttcttattat ttctccacga tcatctaatt
      181 ctggaacaat tggatatttt tctctgaaca agtctagttc tttcaccacg tcattaaaca
      241 aatcttgctt atcttcttgc gtaagattct gtttaatata ctcatctaaa gcttttctaa
      301 gcaattcatc tcttgtcata ttagtctcat acttttctac taaaactctg gaagtcgcat
      361 ttaacaaagt cgacagtttt aaaggatgct tggtggaata aaccttatca aactttctga
      421 ttggtgcgcc aacttttttt gttcttcctc tactctgtct ttcaacattg gaggaagttt
      481 ttatttcttg aacttggtgc acttctaatt gcttattttc agatggtgta attgcttgct
      541 gctttttctt tgcttctgct tcttttttta ttttggcgag tcctttgttg ttcccaaaaa
      601 tatcaagtcc tttgatgtca gccaattact ccacctccgc ataccaattc tcgtgttctt
      661 ccaattcttt caaaatactg ataaataatt cgtggctttt gcgatcccat tgttttaaga
      721 gacctttctt tgtcttctcc atcgtaatac ctgatccgtc gtatcgtttt aagcgcgcat
      781 ttttgagtac aacattttta atcaaatgtt ccccataaat ctcttgggct tgtttataca
      841 tttcctgatc tactgcgtca ccgctttcca acatgaaagg aataacacca gcaacatata
      901 aatttgtatc aaattctttc acaaaaaagt ccatatactc aagatattgc atcgcatttc
      961 tcatcgattt tacttgggtc tgtaagacga cgattgccca gtccgcaatg tacatcgcgg
     1021 atttactata tacagaaata gaaggtggga catctaaata gatgacgtca aactcatcaa
     1081 ataatggttc tagcttttct ttcatatatt tgaattggga caatgaatca ttatattggt
     1141 ctttgatcca atcatcatac atttcaaagg acatgtctgc tggaagtata tagaggttat
     1201 tgattacttt atagacagcc tctttaggtg gaagattgtt tatcaacacg tcagtcacag
     1261 tattttcgta aacaatcggt tcaccttctc cgcctccttc ttcgttagtg aagttcatat
     1321 gcgttaagtt catcacatca gaagtatttc cttgcatatc caagtcaatt atcaaggttc
     1381 tttttccttc attaactgct gattcaaacc ctaacatttg cgcgcacgta cttttaccta
     1441 cgccaccttt aaaattaccg attacatatt tcttcgctac cacgttccta cctcttttct
     1501 tttagcaata ttcatttgat actattataa catatttatt tttagttact accaaaaaaa
     1561 agatatatat gtactacctt ataattaata agtaaaaggt agtacataaa taaggaaaag
     1621 ctgaaatata ttgatttata gctttcctca aagtaatgac gttatttaat ttatcgagaa
     1681 gcagagaata ctcgcgactt tagtcatgta gatgaatcag atcttcgata aaaaagaagc
     1741 cgtccttctt aagaacgaca acaaatcact agattaattt tttcaactgt tcataaaagt
     1801 tttcatgcgt tccacataac aatactggaa taaccttgtt ttctataatc tcgtaagcta
     1861 ctctatatgt tgtgcccgca tatttgaacc cactagacca aatacctgat aaatccccag
     1921 ttttttgttc acccttatat ggatcaatcg caatttcatc ataaattaaa ttcaaaaatt
     1981 tttctttcaa tgtcttctct tttagctttt ttaaatactt aatgacatgt tttgaaggaa
     2041 cgatttcata agccaatttc tgttgccaac tcctctttag tcattacttt agaattagta
     2101 agtgtatctt cagcaatatc tctaaaggaa gaatgcattt tgttttttct attcttaaat
     2161 tctacaagaa tctcatcttt atcgtagcct tctgcaatta tatcagacaa aatatcttcg
     2221 ctaaaatcat aaaattcttt ttgtggttca acaaattcat aaagaatacc attctctacc
     2281 aactttgctt caacttcgac tccattagga acattaaaat ctttaggtac tgtcaacatg
     2341 attgaattgc cttgttttct tgtttttgta atcatttaaa tccctcattt ctaacagtat
     2401 gtaattactt agtaaataca ttataccacg agataatgta tttacaagta attactgtga
     2461 ctcgtaatgc gtccttgctg aataaatttc aaccacgtgt tcatcttcat ctaccttata
     2521 aacaacccgg tgttgtctat tcaatcttct agaataccta ccttcatgtg taggttttaa
     2581 cttctcaaat gattgggtag ggagataggg atcttccttt aaagtctgaa taaccttctc
     2641 aaattgaggt ttcaaattag actgtttgat tttctttaaa tcagctttag ccgagttttt
     2701 aatcatcaca gaatagttat tcattataag ttatccggtt ctgttgcgaa gtttgaaaat
     2761 caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat
     2821 ttcagtagac accgaaaagt cgaagagcgt tccatttctt cggttctttt tatatattcc
     2881 tcgaatggtc tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt
     2941 atttcgtcgt ttaataggtc gatggtcttg ttctattaaa ttgttaagat acttcacagt
     3001 tcggtgctct gtcttagtat ataaacccac actctgtaac tttctaaagg cggagccaag
     3061 agaaggtgct ttatcggtca caattgcttt cggctcacca aactgtttat ggagtcgttt
     3121 taagaaagca taggctgctt gcgtatcccg tttctttcgt aaccagatat ctaaggttaa
     3181 gccgtccgca tcaattgcac gataaagata atgccaacgt cccttaattt tgatataggt
     3241 ttcgtccatt ttccatgaat agaaggattg tctatttttc ttcttccaaa gataatagag
     3301 gactttgctg tactcttata cccaacgata aatcgtagta tgacaaacat ttattccacg
     3361 atcatataac aattcctgaa cttcacgata gcttagattg taacgcaggt agtaaccaac
     3421 agcaacaata atgacgtctt ttttgaattg tttgccttta aaatgattca ttactctgtc
     3481 ctctctgtct tttttctcaa ttttacacta aaatagattt tttggaaaac tttgcaacag
     3541 aaccctttta ttgaaagtaa gaagaacagg cttttttatt agaggcgttc aaaattgttt
     3601 gagcggttag cttttatttt ttagaaataa tttcaaaagt atttttgttg tgtaaagaaa
     3661 gttgtgccaa ccactttatt tttaatgttt caggatattt atggaaattt agcttatttg
     3721 caccaatcac aatggctccg aatgaaacca gtattatctc atagccaaga ttttgaatat
     3781 cggttaaaac atcatccatt ttatcgaaag attagatatt atttttacca attgtatttg
     3841 ttaaagttgt atttccaact ttaattacaa tcctgtctct atgaatgttc atcaggatat
     3901 ttttacaatt tccatttgat gttttgtata gggtaataac tttgctatta tttcatttgt
     3961 tgaaatcttc aaatatccag tagtagtccc atcactacaa ccataccacc tgtcatccgt
     4021 tacatcttta tcaaatatga ttttaatttt attctttgta tcaattagtg tgctaaaaac
     4081 tgtagctgct ccaattttgg tttgtagcat acttgtcata tcttctttgg aagcaaaaga
     4141 cactcttgag attcctaaag ctttactaaa atctttagat ttaaatggtt tgtcgccact
     4201 cgtaataaat aaatagtaat ttgttttttg tctattacac aaaaagagtg tttttaccat
     4261 atgcatatct aatttcaaat tcacctgttc gcaatcttcc atagttatca cttcgtctgt
     4321 ttctacacgt tcatacgata tgtctaaact ttctaaagca gcgtaaactt tttcttgtaa
     4381 aagtgtctca tatttttgag gtttattggt ttttatttca ctaacattaa tcaatctgaa
     4441 tccttctttc acttaatatt tactacaaag aaactttatc atgta
//
LOCUS       DAZKCZ010000128         4467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000128 DAZKCZ010000000
VERSION     DAZKCZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.131"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..1429
                     /locus_tag="ACG8KN_002362"
     CDS             95..1429
                     /locus_tag="ACG8KN_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9189366.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1470..2381
                     /locus_tag="ACG8KN_002363"
     CDS             1470..2381
                     /locus_tag="ACG8KN_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9189367.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2378..3232
                     /locus_tag="ACG8KN_002364"
     CDS             2378..3232
                     /locus_tag="ACG8KN_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9189368.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            3229..3846
                     /locus_tag="ACG8KN_002365"
     CDS             3229..3846
                     /locus_tag="ACG8KN_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP9189369.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3848..4366
                     /locus_tag="ACG8KN_002366"
     CDS             3848..4366
                     /locus_tag="ACG8KN_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9189370.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1340 a          763 c          902 g         1462 t
ORIGIN      
        1 ttagaatcat accttataaa ttgttatttt cagaacaaaa tgatatcgtt atattaaaac
       61 cggcatcggt tacattaaaa tttggaggta aaatatgaaa aaattattgt ttacagggtt
      121 gctggcaagt tcagcattgt ttttattagg aggatgcgga tcaagtaatg caagcgatgc
      181 cagttcaagt gcagcaaatt cttctgaaaa aacaagtgat ggaagaaaaa atattgattt
      241 ctggtccttt tggggatccg gtgcacgaaa agaagctatc gaagaaatca ttgaggattt
      301 caatcaatca caaaacgaga tccatgtaaa ttataagtat cagccatggg gtgacatctg
      361 gactaaatct ttatctgcaa taacagctgg aaatgcaccc gatgttattg tacaggacat
      421 aaattccgtt gcccaaagag cagaagcaca gcaagcaact aatctctcca aatatgtaga
      481 aaaagacact tctaaggatt tttatccgca gctttgggat acagtagaat atgaaggtga
      541 tccttatgcg atcccattca atacagatac acaagtcatt ttttataata aaaagttatt
      601 taaagaagct ggaatcagtg aacaagatct accgacaact tgggatgaat tagaagaaac
      661 agcacgtaaa ctagataaaa aagaaggaaa agaatttaca cgcatcggtt tttatccatt
      721 atggaattta ggggctgatg tatgggcgct aaacgcagat aacgggacta gttggtttga
      781 caaagatgaa aaagtcaaga ttgatactaa aaataaagtg gaagccttgg agtggatcca
      841 agactggcaa gattattatg ggaaaaatac gatcaataaa ttagaagccg aatttgggtc
      901 tggtgtatct gatccattta tttccggatt agtagccatg cgtgcccaaa atatcaatta
      961 ttactctagt ttgatggaaa atgcaccgga tgattttgat tttggcgtga tgcagctccc
     1021 agaaaaagaa gaaggttcag gccattggtc gtggggtggc ggattcgtct tggaagtacc
     1081 atacggagca aaagatcctg aggcctctta tgaattcatc aaatatcttt cttctccaga
     1141 agtacaagaa aaattcggcg aaaaaagttt tgatatcatg gcgagcaaga cagccaatga
     1201 gaatttagtg aataatgaca acctaacgga taaaggaaaa atgatctatc aaatggcgga
     1261 cgaaaacttt aaaaatacgg tgatcacacc agttccttta gatgctccgg attacacgac
     1321 attagtgaat gaacagattg accagattct tttaggaaca aaatcaccaa aagaaggatt
     1381 ggcagatgca caaaaagcag tagaaaactt agtcaagcaa aatcaataat ccatttttga
     1441 ctgtctaggt acagaggagg caagaaaatt tgaagcgttt atctatccga aagaaaaaag
     1501 aagcaagatg ggcctattta tttatctcgc cttttattat cgggttcttg gtatttatgc
     1561 tagggcctat gcttttttca gttattggta gtttcaccga ttataatttg acatccaaga
     1621 tgaatttcat tggtctagca aatttcaaaa gaatgttcgt tcacgatgat ttattttgga
     1681 aatcgttata caacaccgtt tatttcgtca tcttcaatgt accgctgacg acgattttca
     1741 gtgtgttttt agccacagta ctcaatcaaa aaatcaaagg aatcagtgta ttccgtacag
     1801 ctttctattt accggcagtc ttatcaggag tagcagtcta catcttatgg atgcagctgc
     1861 tttctccatc tgcaggattg atcaacacat ttctgggctg gttccatatc cctggaccag
     1921 cttggctgtt tgatccacaa tggacgaaac ctgcgttgat tttgatgaaa atatggagtt
     1981 ctggaggagc aatgctactt tacttagcta cattacaaaa tgttcctaga tctttatatg
     2041 aatcagcaga aatcgatgga gcaggcgttt ggggaaagtt caaaagtatt actttgccat
     2101 taatcactcc tattattttt tacgatgtga tcacctctac gatcggttct ttccaaattt
     2161 ttcaagaagc ttatgtcatg acgaaaaatg gaacaggcgg acctgcaaat tctttgcttt
     2221 tttataatct gcatatgtgg aacaaggcgt tcgtagcttt tgatatgggt tatgcgatgg
     2281 ctatgtcaat gatcctgttt ctgatcgtgt tggttctgac gtttgtcaat ttgaagcttg
     2341 cacctaagtg ggtctattat tctggaggtg acggcaaatg atggattatt tcaaaaagac
     2401 cagtgtgaat cggcaaacaa aaaaaatact aagttatgtc ctcatgacag taataggaat
     2461 cattttgatc attccattgc tttggatggt gtttacttca ttgaaaccaa tggaagaaat
     2521 cgttcgttat cctccgacct ttttcccaga aaaaatcgta tgggaaaact atctggatac
     2581 gattgccgcc tttccttttt ggcgctatgc aagaaataca ttattcatca ctgtgctagt
     2641 agtgatcggt aatgtccttt caaattcttt tattgcttat ggatttgcga agcttgattt
     2701 tcctgggaaa aagctgatgt ttgctttggt tctttctacg atgatgatcc cggggtttgt
     2761 aacgatgatc cctcaatatg tattattctc caagatcggc tgggtaggta cttatttgcc
     2821 cctgatcgtt ccatcgttct tcggcaatgc gttcaatata tttctgatgc gtcaatttta
     2881 cttatcgatc aataatgaat tgatcgaagc tgctgaaatc gacggagcga atcatttgta
     2941 tatttggagt catttgatgc ttccattgac gaaacctgcc ttgattacca ttgcgatcaa
     3001 ttccttcaac gcagcttgga atgatttctt aggtcctctg ttatatatcc aagatcaaga
     3061 aaaatacacg ttacagattg gcttgcaagt ttttcagaac caagcaacga cacaatggaa
     3121 ttatctgatg gcaggagcaa cacttgtctt gatcccgacg attttgctgt tcttttttgc
     3181 acaacgttat ttcattgaag ggatggattt gacaggaggt tcaaaaggat gatgaatcga
     3241 tcgattggta aaaaagccaa tgattatttc ttagaaatca ttgaatggat tccacgtctt
     3301 atccatttac agctactatg gttcctgtgc gtcttgcctg ttttcacatt aggaactgca
     3361 agtcgaacag ttctgtatat gatttatcat tacaaaaaaa atgaagagaa aaagctcggt
     3421 tcactattct ggaaaaagtt tcgtgagaac ttctctcttt atggaaagca agatagttta
     3481 gcaagtgttt attttcttct tctattgatc gattatcaga ttttccgcta ttggggtggg
     3541 ggaattggct atacattgat gtatactacg ttgtttttgc ttctattaag cgcgcttctc
     3601 tttagttaca aaatcttgct tcaactggaa caaaaagaag cttcttggat tactgcattt
     3661 ttcttgtttt ttaatgattt caaaagcgcg cttttctatt tggcaggcac gttgatcctt
     3721 cttgtcgcat tctggtttgc aggaccaatc tattttgctt tattaggttt cagtttcttg
     3781 ttttattttc aagtcctgct ttttatccat cgtacgcaag aaaaggttct taaaaaggag
     3841 gaataacatg ttgcgtttga ttgctaaaat cgatgatagt attatcaaca agttcgccaa
     3901 gcaacggaaa acggatagtc gaaaaatcaa ttttttgaaa tttatcagta atatctctaa
     3961 tggtggtttt atttggttgc tccttgctgc tgttttagtc ttgatacctg atactagaga
     4021 atatggtcta gccattctta tcggcagttt gatctatttt tttgttggtt tgctttttat
     4081 caaaaaaacg acaagacgga aaagacctta tgaaaaaaat cgtcggcttg tcctttacac
     4141 aagtaaaccg aagacttctt cttttccttc aggacataca gcgctagcct ttttatcagt
     4201 agctgcgttg ttcgaattaa gtccagttgc aggaatatta gcctttttat ttgcttgttt
     4261 gatcgcttat tcgaggattt ttttactttt acattatcct actgacattt taggaggagc
     4321 tattttagga agtgttattg gcatcctatg cgctgccgtt atctgaatta attgtacgaa
     4381 taaagcatga gtgcgtaaat gcgcatttgt gctttatttt tttgtttcga ggaaagcgta
     4441 gttgtttatt catttgtctt aataact
//
LOCUS       DAZKCZ010000129         4448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000129 DAZKCZ010000000
VERSION     DAZKCZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.132"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..1665
                     /locus_tag="ACG8KN_002367"
     CDS             175..1665
                     /locus_tag="ACG8KN_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9189371.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            2013..2183
                     /locus_tag="ACG8KN_002368"
     CDS             2013..2183
                     /locus_tag="ACG8KN_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189372.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            2197..2814
                     /locus_tag="ACG8KN_002369"
     CDS             2197..2814
                     /locus_tag="ACG8KN_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9189373.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            2814..3752
                     /locus_tag="ACG8KN_002370"
                     /pseudo
     CDS             2814..3752
                     /locus_tag="ACG8KN_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         1629 a          693 c          823 g         1303 t
ORIGIN      
        1 tataagttaa tagaaaataa caaaataatt tattcgatta gtggaaaaaa attgacttat
       61 aaaggaaaaa aatctttttc aaaacatgca atattgaaac agttgaatga aaaagcaaac
      121 caagttaatt aaacaaccta ttttatagga tttataggaa aggagaacag ctgaatgaat
      181 atcccttttg ttgtagaaac tgtgcttcat gacggcttgt taaagtacaa atttaaaaat
      241 agtaaaattc gctcaatcac taccaagcca ggtaaaagca aaggggctat ttttgcgtat
      301 cgctcaaaat caagcatgat tggcggtcgt ggtgttgttc tgacttccga ggaagcgatt
      361 caagaaaatc aagatacatt tacacattgg acacccaacg tttatcgtta tggaacgtat
      421 gcagacgaaa accgttcata cacgaaagga cattctgaaa acaatttaag acaaatcaat
      481 accttcttta ttgattttga tattcacacg gcaaaagaaa ctatttcagc aagcgatatt
      541 ttaacaaccg ctattgattt aggttttatg cctactatga ttatcaaatc tgataaaggt
      601 tatcaagcat attttgtttt agaaacgcca gtctatgtga cttcaaaatc agaatttaaa
      661 tctgtcaaag cagccaaaat aatttcgcaa aatatccgag aatattttgg aaagtctttg
      721 ccagttgatc taacgtgtaa tcattttggt attgctcgca taccaagaac ggacaatgta
      781 gaattttttg atcctaatta ccgttattct ttcaaagaat ggcaagattg gtctttcaaa
      841 caaacagata ataagggctt tactcgttca agtctaacgg ttttaagcgg tacagaaggc
      901 aaaaaacaag tagatgaacc ctggtttaat ctcttattgc acgaaacgaa attttcagga
      961 gaaaagggtt taatagggcg taataacgtc atgtttaccc tctctttagc ctactttagt
     1021 tcaggctatt caatcgaaac gtgcgaatat aatatgtttg agtttaataa tcgattagat
     1081 caacccttag aagaaaaaga agtaatcaaa attgttagaa gtgcctattc agaaaactat
     1141 caaggggcta atagggaata cattaccatt ctttgcaaag cttgggtatc aagtgattta
     1201 accagtaaag atttatttgt ccgtcaaggg tggtttaaat tcaagaaaaa aagaagcgaa
     1261 cgtcaacgtg ttcatttgtc agaatggaaa gaagatttaa tggcttatat tagcgaaaaa
     1321 agcgatgtat acaagcctta tttagtgacg accaaaaaag agattagaga agtgctaggc
     1381 attcctgaac ggacattaga taaattgctg aaggtactga aggcgaatca ggaaattttc
     1441 tttaagatta aaccaggaag aaatggtggc attcaacttg ctagtgttaa atcattgttg
     1501 ctatcgatca ttaaagtaaa aaaagaagaa aaagaaagct atataaaggc gctgacaaat
     1561 tcttttgact tagagcatac attcattcaa gagactttaa acaagctagc agaacgccct
     1621 aaaacggaca cacaactcga tttgtttagc tatgatacag gctgaaaata aaacccgcac
     1681 tatgccatta catttatatc tatgatacgt gtttgttttt tctttgctgt ttagcgaatg
     1741 attagcagaa atatacagag taagatttta attaattatt agggggagaa ggagagagta
     1801 gcccgaaaac ttttagttgg cttggactga acgaagtgag ggaaaggcta ctaaaacgtc
     1861 gaggggcagt gagagcgaag cgaacacttg attttttaat tttctatctt ttataggtca
     1921 ttagagtata cctatttgtc ctaatatgat tttagcagta taattgactt ggtgaatagg
     1981 tcatttaagt tgggcataat aggaggagta aaatgaaaaa atttatttat cgagttttag
     2041 aaaatgacga agtggtggct atttttaatg agcaacaata tgcgcaagat tttatcgctt
     2101 acgaaaagac aatttctgat aagcaatttg aaattgaaaa agtagatatt gctgattggt
     2161 tattgcaacc gagagaattt tagaggttgg ttgaaaatgg ctaaaattgg ttatgcacgt
     2221 gtcagtagca aagaacagaa cttagatcgg caattacaag cgttacaggg cgtttctaag
     2281 gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc cacaattaca agctatgctt
     2341 aactatattc gtgaagggga tattgttatt gttactgaat tagatcgatt aggacgaaat
     2401 aataaagaat taacagaatt gatgaatcaa attcaaatta agggggcaac cctggaagtc
     2461 ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt taaggcgttt gattaatagc
     2521 cttgtcattg aattgtacaa gtatcaagca gaatcagaac gaaaaaaaat taaggaacgt
     2581 caggcacaag gaatcgaaat tgctaagaaa aaaggcaaat tcaaaggtcg tcagcataaa
     2641 tttaaagaaa atgatccacg tttaaagcat gctttcgatt tgtttttgaa tggtttatcc
     2701 gataaagaag ttgaagaaca aactggaatc aatcgccgaa cgtttagaag gtatcgagca
     2761 agatataacg tgacagtcga tcaaagaaaa aacaatgaaa agagggatag ttaatgagta
     2821 cggttatttt agctgaaaaa ccaagccagg cattagccta cgcaagtgct ttaaaacaaa
     2881 gcaccaaaaa agacggttat tttgagatca aagacccact atttacagat gaaacgttta
     2941 tcacctttgg ttttgggcat ttagtggaat tagcagaacc aggtcattat gacgaaaagt
     3001 ggcaaaattg gaaacttgaa tctttgccga tttttcctga tcgatacgat tttgaagttg
     3061 caaaagataa gggaaagcag tttaaaattg ttgcagaact tctcaaaaag gcaaatacaa
     3121 ttattgttgc aacagatagc gacagagaag gtgaaaatat cgcctggtcg attatccata
     3181 aagcaaatgc cttttcaaaa gataaaacat ttaaaagact atggatcaat agcttagaaa
     3241 aagatgtaat ccgaagcggt tttcaaaatt tgcaacctgg aatgaattac tatccctttt
     3301 atcaagaagc gcaaacacgc caaattgccg attggttgat cggcatgaac gcaagccctt
     3361 tgtatacgtt aaatttacaa cagaagggcg tacaaggtac attttcacta ggacgtgttc
     3421 aaacgcccac cttatacctt atttttcagc gccaggaagc catagagaat tttaaaaaag
     3481 aacctttttt cgaggtggaa gctagtataa aagtaaacca agggtcgttt aagggcgttc
     3541 taagccccac acagcgtttt aaaacccaag aggagctttt agcttttgtt tcttctaaac
     3601 aagctaaaat aggcaatcaa gaggggataa ttgctgatgt tcaaaccaaa gagaagaaaa
     3661 cgaatagtcc gagtttgttt tctttaagta gtttgcaatc aaaagtcaat cagctttata
     3721 aagcgacagc gagccaaact ttaaaagcta tttctttttt aataacttaa aaataaactt
     3781 aatgtaacag caagcacagt caaggtatac acctttgaca aaaaatagca cattctctat
     3841 cgaaaatttt tgcttatttt ttaaattatt ttgggaaatt ttcccaatcc ctttttctaa
     3901 ctcaaaaaat ataatcactc aaaatttaaa agggcgcact tatacatcat tttaaaaaat
     3961 tgatgtaacg tgctaagttc aaaacaaagg gcgcacttat acacgatttt caatcttgta
     4021 tatttctaac gaaaagcgtg cgccaaaaaa cccccttcgt caattttgac agggggcttt
     4081 ttgatgtaaa aatttctatc gaaatttaaa aattcgcttc actcatgtta taaagactta
     4141 aaataaaata actctttaaa atcttttgct agttgttctt caatattttt tattcggtgc
     4201 atcttccaag taaagtataa cacactagac ttatttacta cgtttcataa gtcattaatg
     4261 cgtgtgctct gcgaggctag tttttgtgca agcacaaaaa atggactgaa taaatcagtc
     4321 cataagttca aaaccaaatt caaaatcaaa accacaagca accaaaaaat gtggttgtta
     4381 tacgttcata aattttatga tcacttacgt gtataaaatt aaattcactt tcaaaatcta
     4441 aaaactaa
//
LOCUS       DAZKCZ010000130         4139 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000130 DAZKCZ010000000
VERSION     DAZKCZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.133"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..793
                     /locus_tag="ACG8KN_002371"
     CDS             29..793
                     /locus_tag="ACG8KN_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFP9189374.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            780..2222
                     /locus_tag="ACG8KN_002372"
     CDS             780..2222
                     /locus_tag="ACG8KN_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9189375.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            2233..3561
                     /locus_tag="ACG8KN_002373"
                     /pseudo
     CDS             2233..3561
                     /locus_tag="ACG8KN_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            3574..4032
                     /locus_tag="ACG8KN_002374"
     CDS             3574..4032
                     /locus_tag="ACG8KN_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9189376.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1389 a          606 c          891 g         1253 t
ORIGIN      
        1 ttttagtaga aaaaagaagg agaatgaaat gggaaaagtg atttttaatt cggatgattt
       61 tggctatagc catggtgtga attatgggat tatggatgct tatcagcgag gaattttgac
      121 atcaactaca ttgatggcaa atatgccggg tttcgagcac gctgtgaaac taagaaagga
      181 aatgcctagg ctaggtgtag gagtccattt aacattgacg tgtggcaaac cacttctcaa
      241 aactgtcgat actcttatcg aaagcggaga gttcagatcc ttatcttatt atcagcatcc
      301 atttaacatt gacaaagatc aactgtatca ggaatggaat gctcagattc aaaaaattta
      361 tcatgcgggg atcataccaa ctcatttaga cagtcatcat catactcata ctttggatga
      421 aaaccaggag gtagttgtta cattagcaaa aaaatatgat ttgccggttc ggagcaactt
      481 tgaacgaaaa gatgaagttc gtcatgtcga ttattttgag ccttgtttcg atgtcgtagg
      541 agaaactgat gaaaaaaaac gacaaatcaa tacgagtgtg gaagaatatt tggcaagttt
      601 actcatgata ttgagagaag ataaaacgat cgaagtcatg tgtcacgcag cgtatttgga
      661 tcaggaattg ttaaatggtt caagttttgt gtatcctcgt actcaccaag tcgaattttt
      721 aattcattca gcatttgctc aaaaagtgaa aaatgatcct ttgattgagt tagtaacata
      781 tgaggatatt taatagagga ggaataaaaa tgaatgttaa atcaaatgtg cagaagcttg
      841 gaagagcgat gctacttcca gttgcagcga tgccattagc gggattgatc atgcgtttat
      901 cagctgatga tatgctgaac atcccagtaa ttggtgcagc tggaaatgcc gtttttggaa
      961 acttggatat cttatttgct ataggagttg caatcggttt tgtcaaaaat aaggacaaag
     1021 gaatacctgc acttactggt tttttagcga ttgctacctt gaaaaaaggc ttagaaatca
     1081 tggacccaga agtgaatatg gggatttttg gtggaattat ttccggttta gtagcggcat
     1141 ggacgtataa tcgctttaaa gaacaaaaat taccagtagt tttttcttat tttgcgggcg
     1201 aaaaattccc attgactatg gtgatgattc tacaaacaat cgcagctatt tttttgggct
     1261 ttatctggcc gctcatccaa attgggattg ataattttgc aaaactgctc gttgatatgg
     1321 gggcattagg tgtggggata tttatgtttt tgaatcgttt gctgatcccc tttggactgc
     1381 accatgtgtt gaatacgtat atttattacg atttaggaag ctatacatct cctagcaagg
     1441 aagttttcag aggagagatg acacgcttta tcaacggaga tcctcaggct ggactgtttt
     1501 tatcaggttt ttttgtagta atgatgtttg gtataccagc tatatgtcta gctatctata
     1561 aagcagctta taaagaaaat aaagagatgg tcaaaggaat catgggaagc ggtgcaggaa
     1621 cttcatttat tgcaaatatc actgagcctg ttgaatttag tttcatgttt ctttctccta
     1681 tgttgtacgt gatccatgca ctttttgccg gtttagcagg gattgtctgt tacttattca
     1741 atatacgaat tggttttaca ttcggagcat gtattgttga ttatctcatc aattttcgaa
     1801 tcgctaccaa tgctattttg attcttccaa tcggaatttt cttttttgcc ttgtattacg
     1861 ttacttttta ttatttgatc aataagagaa acattcagac attaggacgt gaagctaagg
     1921 cagagtttgg caatgaagta accttagaag aaacagagtt agggttagca agtaaaaatt
     1981 atgaatatat ggcaacaaaa atgctacaag cgtttggagg aaaagccaat atcctggatg
     2041 tctatagttg taatacacgc ctacgggtag aggtagtgga tcccaccatg gtcgaggaac
     2101 agcgaatcaa gcaattaggg ataagcggga tcattaaacc aactgaaaaa aattatcaaa
     2161 ttatcatcgg cttagaagtg acttatgtta tggcagaatt caataaatta ttagaagaat
     2221 agaaaggaaa agatgatgat gaaaaaacag attatcacta tagctggagg cgggagtacg
     2281 tatacaccag gaatcgttca agccattttg aacaatgaaa aacggttgcc attatctgaa
     2341 atacgtttat gatattgatg aagaaagaaa tatggatatg tacttgattg ttaaatttat
     2401 gctgaaaaaa aaggtttttc aaatattcgc attcgtgcaa cggatgatcc aaagcttgct
     2461 tttacaggat gcgattttgt tttctcacaa attcgtgttg gaggattgga gatgcgagaa
     2521 aaggacgaaa agattccact taaacatgga ttagtaggac aggaaacgtg tggactcgga
     2581 ggatttgctt atgggatgcg ctcaatcaaa ggtttattgg aaattgtcga tcatatacaa
     2641 gactatgccc ccaaagcatg gattttgaat tatacaaatc cagagtcgat cgtttctgaa
     2701 gctgttcgtc gtaaatatcc gaatgtaaaa atgattaatg cctgtgatat gactatttca
     2761 attgaagaaa cgattgcagt gaattacggt tatgatcgta agaattggat cgttacgtat
     2821 tatggtttaa accactttgg ctggtataca tcgatttatg ataaagaatt gcaacgagaa
     2881 attatgccag agattattga aaaactaata acaaaagaaa tgcaagtcgc cgattttaat
     2941 ataggagaca aaacttggca aaaaaccttt caaatgatgt cagtcatcac gaaaaatttt
     3001 ccaagtaata tcccgaataa ttacttagag tattacttat atcctgatat ggtcgttgag
     3061 catactgaca aagagtatac acgtgctaat atggtcatgg atggacgaga gaaaaatacc
     3121 aaagaaatgg ctgataaaat acgccgcggt attgtggaag aagtattgaa ctttaatttt
     3181 ggggagcatg gacaatatat cgtcgatata gctatttcct tgttgaatga tgatcgtcgt
     3241 cgctttatgc taatcgttcc taatcaaggg gcgattccga atctacggtc agatgcagtt
     3301 gttgaaatac cggcatatgt tggtgcaact ggtgtggaga caattacact aagaaaacca
     3361 atcagtgatt ttcataaagg attgatggag gcacaagttg cagcagagaa gttactagta
     3421 gatgcttact ttgaaggttc ttatcaaaaa gctttacaag catttacatt gaatcaaacc
     3481 gtaccaaatg ccagagtcgc aaaaaaaata ctagatgaaa tgatcaaagt aaacaaaaaa
     3541 tattggccag cattaaagta gaggtgataa aaagtgcatt tctttaaaac aagaaacatt
     3601 ctaaaagctg tagctagtgg tcaaattatt ccattaacag cagtaaaaga cgaggtcttt
     3661 tcttcaaaaa tgataggagt tggatacgct ataacgaatc atgatggata tatccatgcg
     3721 ccagttaaag gaataatcga taatatattt cctacccgac atgccataac gatcgaaagt
     3781 gaatctggca gaaaaatact gatccatatg ggtttagata cagttgaact cgaaggaagt
     3841 ccgtttcaga tacgggttga aagtaaccag gaggtaaaag aagggacact gttagctatt
     3901 atagatttga agaagttgga gcaagcacaa aaagatacgg tgatcattgt agtttctcca
     3961 ggcatttttg aaggaaattt attaaaacaa aaccaagaag taaagattaa agatagttgt
     4021 tttgaatttt agtgtacgat gaaaaaagtt aaatagggtg atatttatgg gtgaatttac
     4081 aaaatttttt tgaaatgata agtttcaatc actagattta atcattttga tttttgtta
//
LOCUS       DAZKCZ010000131         4083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000131 DAZKCZ010000000
VERSION     DAZKCZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.134"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1348
                     /locus_tag="ACG8KN_002375"
     CDS             47..1348
                     /locus_tag="ACG8KN_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9189377.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1375..1635
                     /locus_tag="ACG8KN_002376"
     CDS             1375..1635
                     /locus_tag="ACG8KN_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9189378.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2634..3869)
                     /locus_tag="ACG8KN_002377"
     CDS             complement(2634..3869)
                     /locus_tag="ACG8KN_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9189379.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1163 a          665 c          852 g         1403 t
ORIGIN      
        1 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
       61 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      121 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      181 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      241 aacgattgtt ttacctttta ggatgacgat gggattgatg gctatttatg caacttttgt
      301 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      361 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aaaaggcacc
      421 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      481 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      541 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      601 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctac
      661 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      721 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      781 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
      841 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
      901 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
      961 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1021 caatgagccc atgatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1081 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1141 caatagattt actgttttgc caacttggac attaccagct ccaatcggcg gttatctttc
     1201 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1261 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1321 tggagaaaac gaagtccaaa ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1381 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1441 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1501 atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1561 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1621 acatcgaagc tttaaatgtt ctaaaagtgg ttgtgacaga agtggttagc ttcaagaaat
     1681 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1741 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1801 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     1861 tttggcagaa agaaggaggc aaaaatgaaa attttatcag caatggatta agaattagtc
     1921 gatctactac caaagtgatc aaggcaaata tagatttggg agcttacaac catatagtag
     1981 gggctgcagt atggtgtatg aatggtaata tgtatgatga gagtgtttat tcaaatcatg
     2041 aaacgtgtat agaaataatt gcgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2101 agtttatcgt tgctgacgac atagaacatt ctgacatgat ctactcagca tgtggggatt
     2161 gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg
     2221 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaagg
     2281 gactagcagc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2341 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2401 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2461 ggattgcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2521 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2581 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2641 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2701 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2761 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     2821 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     2881 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     2941 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3001 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3061 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3121 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3181 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3241 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3301 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3361 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3421 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3481 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3541 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3601 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3661 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3721 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3781 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     3841 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     3901 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     3961 tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga
     4021 aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg ttgtgccaga
     4081 taa
//
LOCUS       DAZKCZ010000132         4029 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000132 DAZKCZ010000000
VERSION     DAZKCZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.135"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..643)
                     /locus_tag="ACG8KN_002378"
     CDS             complement(101..643)
                     /locus_tag="ACG8KN_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFP9189380.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            777..1166
                     /locus_tag="ACG8KN_002379"
     CDS             777..1166
                     /locus_tag="ACG8KN_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189381.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1170..1934
                     /locus_tag="ACG8KN_002380"
     CDS             1170..1934
                     /locus_tag="ACG8KN_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9189382.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRGSEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2063..3010
                     /locus_tag="ACG8KN_002381"
     CDS             2063..3010
                     /locus_tag="ACG8KN_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9189383.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQIRSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3140..3880
                     /locus_tag="ACG8KN_002382"
     CDS             3140..3880
                     /locus_tag="ACG8KN_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189384.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKGNLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKMLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1330 a          679 c          822 g         1198 t
ORIGIN      
        1 tgatgagtat atgtcgatgt tttttaatca aaataataaa taaaaactgt ctaacattta
       61 aaatttaatt tagatattag acagttttta agtttgtgag ttattttcct tgtcgttttc
      121 tcactttttt agtccagaaa ccacctgaaa tatattttgg ctcataagaa atgataaaag
      181 cacgctcttc gatttctttg atcaggcgat acagttcacg ttcgcttttt ctaggagaaa
      241 gtatttcgag aactagccgc tcaccttcca gccccattgc agaagtctgt gtcacaccat
      301 aaccgtgttc tctcagtata cccggcaaac ttttatcttc tgcgctattt gctggcaaaa
      361 tcgttgtgac catgatgtat cctaatgcca atttgtcttc aatcaaaatt ccgacactga
      421 tacctactgc gtaacctaac gcatagacaa ataaattcag tggattatct aaacgatcta
      481 aaacaaggga taagccgacg atataaatcg tgatctcgac catactgaca agtggagcaa
      541 tcagtcgata gcctttcatt gtcaacatga aacgtaacgt gttcagagtg atataagcaa
      601 aattaatgag gaagatcatc cccaacattt ttggatcaat catttgtaaa actcctttca
      661 tttatataac aacaatatca aaaaaaatcg taagtgaaga aaaattgaaa ctttatacat
      721 acaaaaaatg ataaaatgat tataatagaa ttataaagat aggttaggtg attaaaatga
      781 aaacggtcaa tcgtgtgttt tgtgccagcc acgtgctaag tctccttctc agtatatact
      841 taggtgtcta ttcgattcaa cagccaattg aggtagagaa cattgttttt cagatgtcgc
      901 tgacagatgg tttaatttta agcgtttttt tctttttgat actgtttatt ggtaatattt
      961 tcggtagcgt tgccagtttt ttgaattttt ctatttatcc actcctttcg ctagcattag
     1021 gagtggtggg actttgttct ctgtgcttat ttcccgagcc attcagtcct gcatttatat
     1081 tatttggtac actgaacctg ttccagacaa ctgttggagc atggttgcta tggcgatcag
     1141 gaaatttaat gaaaataggt gagtaacaat tgaatccaat tatattgaaa cgttctgtcc
     1201 atcatattcc agtgttagaa gttgttctga aagaattaag agaggaacgt attcccgttg
     1261 tcatctatta tcacggatgg cagacaagta aggagttagt tttgacgcaa ggaagaaaaa
     1321 ttgcgcagca gggcattcgt gtgatcctgc cagatgcgat gaatcatggg gagcgcaaac
     1381 agccggtttc ttctattcca tcctatacat tttgggatag tatctacgga aatctgtttg
     1441 aatttgacac acttatcaag catttagaaa aaagaggtct tctacatgat cagcttgcag
     1501 ttggcggggt ttcgatgggg ggaatgacaa catgcgcctt gttggctaaa catcctcata
     1561 tagtagcggg cgtgtgtttg atgggttccc ctgcaccttt ggaatatgga aaagaaatcg
     1621 taaggcgcgg atcggaatat aattatgtcg taccaaaaga ctattttgat ttgatttcat
     1681 gggttaagag ctatgatttg tcattgcagc cggaaaagtt agctggacgt cctctgttct
     1741 tttggcatgg acaagctgac gaaaaaatcc cttatataca aacggaacga tttgtagaaa
     1801 aaaatcggca taagtcttac ggaaaagaaa ttgtttttcg ttcctcaaaa acacagcgac
     1861 atatggtaca aataccacta atggaagaag cttccacttt tttagcaaaa aatttgataa
     1921 aaaagtataa ataattgaaa cggaaaatca ctcatgacga tagaggattt tccgtttttt
     1981 attttgggct ataataaact ctgttaggaa aatttggtat aattgatagt aagaatgaca
     2041 acatgttaga aaggaagaga gcatgcccac tttagcagat gttgcaaaac gtgccaatgt
     2101 ttcaaaaatg acggtctctc gagtcatcaa tcatccggaa caggtgactg acgaattgaa
     2161 agagttagtt ttctcagcaa tggctgaact ggattaccgt ccaaatattg ctgcaaaagc
     2221 acttgtcacg aatcggtcgc agatcatcaa attatttata ctggaagaaa tcgacacaac
     2281 tgagccctat tacatgaatt tgctgatggg aatcgctaaa cggatcggta aagcgcatta
     2341 ttctctccaa ttagttacaa atgatgcctt cgacgtcggt tcttgtgatg gatacatcat
     2401 cacaggtgtc agaaaaaaag acttcgattg gatcgatcgt ttggaaaaac cagttgtctt
     2461 gtttggggaa aattctgaag gatatgatta tgtagacagc gataatgaat atggtacagc
     2521 aaaagcaacg caatatgcct tagaaagaga ttatcaaacg attatttata tcggtatcga
     2581 agtggacgag ccttttgaaa tttcaaggga aactggttat ttatctgtga tgaaggaagt
     2641 tgatcttcct agtaaaatct tgcggtttcc aaatcattcg acagaagtag aaagatatat
     2701 gacagaaaac tgggcagatt ttacagataa aacttgtttt gtatgtagtt cagatcggct
     2761 agcactagga gcagtaagag ggattataaa tgctggagga cacttgcccg aagaatttgg
     2821 tgtgattgga tttgatgggg tatttttaga ccaaatcagg tcacctaaat tgacaacgat
     2881 caagcaagat attatacgta tgggtgaagt atgtggagaa atgttgttaa aaaaaataga
     2941 agaaaaaggg gcgagtcaag gttatcgtcg tttttttcct gaactagtta tcagagagac
     3001 aacgaattaa gtcttattac tatatatata tttatgaaat tcatcgctat tttcttaaaa
     3061 taatcagcgc attgctactg aaaacaatga atctgtgcta ttattgagaa aagttccatt
     3121 tttgagaagg atggtttgaa tgaaagaaat cagacgaaaa catttccgtt ttcctgcgta
     3181 cgatgatgag ttaggcgtaa aattaaccaa cgacaagaag cgagtattat ttgacggaca
     3241 agaagatctt ttgactgaac agaatcagtc cccgtttgag cagatgaaag aatttcattt
     3301 cacgaacacg aacaccgaga aaatccgtcg caagcgtcgt gcaacaaatg gattgaagtc
     3361 agctccgcgt aagggaaatt tggaaaagca taaaggaaaa ttaccggatt ataccggcca
     3421 ttaccaatca gaaatcacat cgacaggacg aaaaaaactt tttggtgatt cctctcgttc
     3481 tgtcagacaa agtgaaacaa ggacagagtc caagcatact acacgaaacg catctgttga
     3541 cacgaaggtg cgggagcgtt cttattttgt tcctaaatat gtacccgcat cgattatccc
     3601 agaccctaga gaaagcaaaa tttctgagga tcatttaatc gaatcgatga aaaaaacgga
     3661 tgactcttat ctgttgttcg atactgaacc gacgccgtat caagaaaaaa gaaatggtga
     3721 gccgtctgta cgaacattca atcgaacaaa aatgttagag gaaacaaagc agtcagaaga
     3781 aaaaaaatcg aaaaaaagcc atggtatttt agaacgctca ttacaaggat tgatcgatga
     3841 tcaaggaaac tctttgggag aaaatagcta ttttaaataa gtgatataca aatttatatt
     3901 cggctacagt tggaaaggaa acgctgcggc gtttccttat tttatactct atccggaagt
     3961 ttatatcgta tcctcagatt tttttgagct taagcgaatt ttttctccca ataagtaaga
     4021 aaataatgg
//
LOCUS       DAZKCZ010000133         4001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000133 DAZKCZ010000000
VERSION     DAZKCZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.136"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(214..363)
                     /locus_tag="ACG8KN_002383"
     CDS             complement(214..363)
                     /locus_tag="ACG8KN_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189385.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            825..1037
                     /locus_tag="ACG8KN_002384"
     CDS             825..1037
                     /locus_tag="ACG8KN_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189386.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1315..3114)
                     /locus_tag="ACG8KN_002385"
     CDS             complement(1315..3114)
                     /locus_tag="ACG8KN_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFP9189387.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3238..3834)
                     /locus_tag="ACG8KN_002386"
     CDS             complement(3238..3834)
                     /locus_tag="ACG8KN_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9189388.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1436 a          710 c          685 g         1170 t
ORIGIN      
        1 aatattatca tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga
       61 acttgtgttt ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga
      121 atacaaaaag acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga
      181 tctcatcagt cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac
      241 tgcttgttcg attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat
      301 aaaacggtta gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt
      361 cataaaattc atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg
      421 aacttatacc aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt
      481 tccttattta ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca
      541 atcatcaagg ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa
      601 taacttagca atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt
      661 tgtcacaacg tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa
      721 aataaaaaat aaaaaaatgg ataaaaacgt tttcaaatac aaggtaattt gttattataa
      781 aataaagaaa aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag
      841 taggaagatc ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg
      901 aacgtacata tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc
      961 aacttatcaa tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt agcgcacctg
     1021 ctgaattggt ttcttaaaca gtatttttga gaattcgcaa gaactccctt ataatataaa
     1081 taaagacaga agggagcgag aagatatgcc aaaaagaaaa agttcaggga aaatgggaat
     1141 tattcttgca gtgatctctg caattcctgc tattttagct attgttcgtg cgatgaagga
     1201 atcaagagaa agttaagcac acattatgag cgtcaaacaa aaaacgcata tacctgcggt
     1261 ggcaggttat gcgttttttg ttgtataaag acttttttca ttaacgattt ttgattagcc
     1321 gactcccaaa acaaaattta gcagtttatc tgaataagtg atttcttcat ctgtttgttt
     1381 cacactttca tttagtagtc ccatctcatc ggttatgata ccatccacac cataaaacat
     1441 catgcgactc atagcatctg aatcgttgat tgtccataca tatacttctt ttccgtcttg
     1501 gtgggcagaa ttaatgaagt tacggttgat cgtactatat tccattgtca gaaaatcagc
     1561 aggcgtcaca gggggcccaa caatattgaa aggtaagatg tagcccacat aaaagtttgg
     1621 ttctgcttct tttaaatctg aaacggtttg gtacgtcaaa ctttgcaaaa tatgatggtt
     1681 ctctaagata actttgcggt atttatggac aaatcgttcg acaaggtcat ccgaatcttt
     1741 tttagaggtc ttgatctcaa taagtaattt ttggtttagc ttatgtgctt tttccaaata
     1801 agaatcaaaa gaacaaacag gggcttgcac tccgttttca gtaactgtaa gatttgtcaa
     1861 ttctttcaat gtaagatcct gtggtgttcc atttacccct gttaagtgac gaagatttgc
     1921 atcatgcatc actacgaaat gaccatcttt tgtttcctga acatccatct ctacataatc
     1981 aggatgatag tctctgcttg ttttttcaag tgcttctaaa gtattttgcg ccccattttg
     2041 ggtactcaca cctctgtgag agacagtcac tggttttgtc tgaacagctg aagtcagata
     2101 atccatattg taaagacaga cgccaatacc gaaaaaaact gcaaaaagag tcaaaccagt
     2161 gtttttcagc gcagagaaac gcaagttggg ggcttgtggg ataaaccact tagggatctc
     2221 aggcaagaag ccttcgtcat ccatgaaatc aacaatgata taaaaaatcc ctacggtaga
     2281 catcacgata ttgaataata aaatcccttg taaaagcgtc atagcaaaca cagcgctgat
     2341 caatgcatag tctgggaaaa attgttcaac catactttgt gccaaaataa caacgatata
     2401 tcctaaagaa gaaaggagta aaatcgtacc gccaatcaca ataaattggc ccgtgatggc
     2461 caaaagacgc gatttggtca atgaccagct ttcccgaatg gctgctcgaa aaggacgatc
     2521 ccgtagaatc atttccggta aagcaaaaat cagtcgaatc ccaatatagc caaggaaaat
     2581 ataaacgagt aaaaaggaac tgacgatgat ccaacgatta gcaaaaataa aatccataat
     2641 gaaagcgggg atttttattt ttgacagtag atcagaatta aaacttaatc cgctgatcgg
     2701 tagaatcaat aaaaaataag caaggaaaaa caaaaaagta atgggtctca ctttcttcag
     2761 ctgaagtatc gtcaagtgta agagttgctt caaagagatt ggttctttct ttttaataaa
     2821 aaaaacactc atcaaaagaa aagtaaattc gaaataaact aacagtaaaa tcgtcatcaa
     2881 aactacgatt aacgagagta gaaccccagg atgttgagaa aaaattgaag gaatattgtc
     2941 ataagacaaa tagtcaattg cccctcggtg taaaataaaa cgggtcgtac tgctcaataa
     3001 gggtaaacaa ataaataaaa taaagccatg catcagcaag acatcacgga aataagcttg
     3061 tgttcctttt aaaaagtccc aagcattacc taagctacgt ttcaaataat tcatcctatc
     3121 acctgctctt cacattatag cgcaaatcta atgaaaaagg aaagggtgca gtgtgttttg
     3181 cagacccttt cccagattta acattgtcaa tcaatgacta attttacaaa ataaatatta
     3241 tccaagaaga ccacttagat attgacctcc ataaatcaag gccatactat ataagaaaat
     3301 cgacagtggt ttagcaatca aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa
     3361 tccagccatc aaacataacg catcgtcagg agcaactgga agaaaaatag ctagagcaaa
     3421 cagcttctca aaacggtttt gattatccag ccagccaata tatttgttat atgtcttatc
     3481 gctaatcatg gaaagtatga atggctttcc ataacgacgc cctagcagaa aaatgataat
     3541 cgaaccaatg cagattccaa tatagttata gataaatcca gcataagggc caaatatcag
     3601 gacaccagct gcagtactga tccctccagg aatgattgga ataactactt gcaggatttg
     3661 gatgaagaca aaaataatcg gtcctaaaat gatggaatca cctactaatc ctcttaacgc
     3721 attcaaatct ttgaaaacac ctaaattaat aaaataaatc gtcagcgcga ttgataggcc
     3781 taatccgata atggatatga aatttatcag ttttcgagag gcagcaatat tcattattca
     3841 tcaactcctt agaggttctt tgttaactat actttactag aaaagcaact aactgcgcca
     3901 ttttttgata tgacagaaaa caaacaattt tgtaagttca tcataagtat aaaagattga
     3961 tctcaaaaaa acatccaact aaagacttat ttaataactt g
//
LOCUS       DAZKCZ010000134         3959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000134 DAZKCZ010000000
VERSION     DAZKCZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.137"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..452
                     /locus_tag="ACG8KN_002387"
     CDS             <1..452
                     /locus_tag="ACG8KN_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189389.1"
                     /translation="LFLSKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIFSVLLST
                     TLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFIT
                     PLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(578..1255)
                     /gene="nth"
                     /locus_tag="ACG8KN_002388"
     CDS             complement(578..1255)
                     /gene="nth"
                     /locus_tag="ACG8KN_002388"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9189390.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1262..1960)
                     /locus_tag="ACG8KN_002389"
     CDS             complement(1262..1960)
                     /locus_tag="ACG8KN_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9189391.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2228..3412
                     /locus_tag="ACG8KN_002390"
     CDS             2228..3412
                     /locus_tag="ACG8KN_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFP9189392.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3418..3861)
                     /locus_tag="ACG8KN_002391"
     CDS             complement(3418..3861)
                     /locus_tag="ACG8KN_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9189393.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1039 a          776 c          644 g         1500 t
ORIGIN      
        1 atcttttttt atcgaaaaca atacctcgtt attccaagaa taaaaaagct gaaaaatttt
       61 gtttattatt aaataaattt actttaattg ctcctatcct tgcatttatt attttttctg
      121 tacttttaag cactactctt aaaggaaaat ttatggaaag aagctctcat gcaatgattc
      181 tcactttttt atggttgttg ttcaccagaa tctatatttt tctgatgagc ctgaaaccgc
      241 ccaaatccat tagtttatgt ttagttataa atggtatttt tttattatca cttatcattt
      301 ttattacacc tttagataga tacgtcactt atttatataa tcctttggaa tactggactt
      361 attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc aataaaaata
      421 acaactttta tttttatcgc aataaattgt aataagttac catcctagct actgagtatg
      481 tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat aataacaaga
      541 taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt ttctttttta
      601 gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat agcaaaggac
      661 atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa atcagtgtat
      721 gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct tctacttcca
      781 tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc acgtgggtat
      841 ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct gtctttctgc
      901 ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca tcgaaacgtt
      961 caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac aaaccaatcg
     1021 tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg ggtgtcggaa
     1081 aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc tgggcactta
     1141 agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca tgtgcttcgg
     1201 gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt aacatattcc
     1261 gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt tgagctgact
     1321 gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct tccacttgtt
     1381 gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg taattaaaac
     1441 tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc ggctgataat
     1501 ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc gggcgtccaa
     1561 attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt ttttcttcgt
     1621 attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg taaagattat
     1681 atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat ccatttgtta
     1741 cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc atagcaatca
     1801 tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt agaacaaata
     1861 aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga ttagatatga
     1921 ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct ccgctcaatc
     1981 acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat ttacttcact
     2041 gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt tttgatgcgt
     2101 atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag aatgttgaca
     2161 aggaaacaaa attactatac actatttttg tttccaagtt aacattattg cataaaggag
     2221 tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg tctctgtctt
     2281 attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa cgctgcctta
     2341 ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg tttatgctct
     2401 agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg ggcgtcatcc
     2461 gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg gtttaggaaa
     2521 ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag gagaaatctc
     2581 caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca aagtttttgg
     2641 atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag ggggtctatt
     2701 agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt tcttaaatgc
     2761 cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt cagtagattt
     2821 aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga tttcatccgt
     2881 tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc ttcaatctat
     2941 cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg gtttaagctt
     3001 ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc gattgctcaa
     3061 aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc tcgcctatct
     3121 attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg ggattatctg
     3181 ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat ccaattctgt
     3241 ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat ctctatcgcg
     3301 cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc ttccgacggt
     3361 attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat agtagcttca
     3421 tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg tgtcaaattc
     3481 ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt gatggagttg
     3541 ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga tttctttttt
     3601 attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt ttgttagctt
     3661 actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt taggatcagg
     3721 gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg caaattcttt
     3781 ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat attcttctat
     3841 tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat tgtttttttg
     3901 tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa accaagaaa
//
LOCUS       DAZKCZ010000135         3954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000135 DAZKCZ010000000
VERSION     DAZKCZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.138"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(602..1159)
                     /locus_tag="ACG8KN_002392"
     CDS             complement(602..1159)
                     /locus_tag="ACG8KN_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189394.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1338..1949)
                     /locus_tag="ACG8KN_002393"
     CDS             complement(1338..1949)
                     /locus_tag="ACG8KN_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189395.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2062..2319)
                     /locus_tag="ACG8KN_002394"
     CDS             complement(2062..2319)
                     /locus_tag="ACG8KN_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189396.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2916..3194)
                     /locus_tag="ACG8KN_002395"
     CDS             complement(2916..3194)
                     /locus_tag="ACG8KN_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189397.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3228..3479)
                     /locus_tag="ACG8KN_002396"
     CDS             complement(3228..3479)
                     /locus_tag="ACG8KN_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189398.1"
                     /translation="MKEKPVNKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1200 a          809 c          526 g         1419 t
ORIGIN      
        1 tggagttaaa cgcttctgtc ccagcctcct tattccgaat aaatggtgtc caaaagcttg
       61 catctgccag attatctcat ttcttatgac aagctaagca aattatctcc accaaataat
      121 ttattcatat ttttgacttg cgcatactaa ctaattctta aattttcata caaaatttct
      181 caatttccct taacgagtaa tatttatgta cggtgattct cactagtatc ccaatcactt
      241 tttaagttat cccgatttca attctacttc aaaaatagtt gcttttatgt atgctgttag
      301 ttgattagat ggtcatttgt tgagctaggt cgtttatttt aaacaagttg tttctttagc
      361 ttatacctat ctcgtctacc aaacgcaaga tggcaaacac aaatgcatac gcatctgtat
      421 ttgccacttt gattcgtata aagctaacaa ctattaactc ctctcagcta atagttgctc
      481 tcttttttat tcagattcat ttttgctgat cacagcctag tatttcaatg gttttgaaca
      541 ctaaaccaaa ttgttccatt acaacattca ctggctgatg aaatggatta gtagatttcg
      601 attattcaaa gcgattttct attggtggat cgtgttccca actaataaga cggattccaa
      661 acctatcgta cagatcttct aaaccactta taaattttgc acctatttca ggtgttttat
      721 tgcgtggata ttctattgtt acgccattaa atggatacca attaggatta ggctctcttt
      781 ttttctgtac atctcccata gaatagttag cactttctgg gacataatat attcttcgac
      841 catcctctaa ttcatctaat aagatatttc gtgatcgtct atcacttgct aaatgtagac
      901 gccacgtgcg ataccatgcc atgctacctg catctgcaga aaatccatca aataatgcaa
      961 caattgtatc ttctatcaca atcctttccc catctacttc atattctaat ctaaaatcaa
     1021 actcccctcg ggtaatttct ggtcttggtg gcttaggacc caatattatt ctacttaaaa
     1081 ccgcattcgt tcccagatga aacacagcta taacaacaat aatgattaat attttgtttc
     1141 tatttgataa atttctcatt attcgcacat cacctctaca ttctcacata ttagttcgta
     1201 atactccttt aatatcaaaa tatctactgg tcgtaataat attcgctatg aattcctact
     1261 actcgtataa gaaaggccaa atcatcagct tttacttgct gatgatttgg cttgtttcta
     1321 tttatcgtaa ttgttttcta atcaccaaaa ttgttaacaa tcggttcaga tatttcccag
     1381 ttgattaagg taattccgta ttgtgtcaaa acttcttgcg cttctccgaa attaagtaca
     1441 gttcttatgt atccactttc tcctaatata aagtctacct ctcttttaaa accttcctcg
     1501 agatttctaa gggctatatg tggttttaat gacattgatt gtacattttc ccccatataa
     1561 tattgcgcac ttcctcgact aaaatagatt cctagatcgt ccccaaaagg tagatcttct
     1621 ctaccactag ctaatctact tctccatctt cgttctcttt tcatagaacc tgcgctgaac
     1681 tcacacccgt taaactcagc tatgacagta tcttcaatta tgttaatctg tccatctagt
     1741 tcgtattcaa tcctaaatgg aaattctcca taggtgattt ccggttcggg tattttaggt
     1801 gagattaatg caaagaaagg aaatattaag cttggcaaca tcaaaaaaac tcctcctatg
     1861 atcacaattc ccgatgtaat ccaaaatata atttgtacaa ttctcattga tccattgtat
     1921 tccattttat tccccctatt actattcata tccgcctatc tatatcgatc ttccaatgca
     1981 gagataattg cttccatacc ttgatttttt actgagttac ctatgcgaga tatcattctc
     2041 cactaaggtg catcatattg tctatctaac atgattaatt ctccaatagg tgtatactcc
     2101 tccaaaaaca acatttaatg catctccctc gacaacataa gctaccatgt ttgctgtata
     2161 tgtgtttgag tctaattcat aagcagataa atttactgtc gctggattaa atataatcgc
     2221 attcgtattt atagctactg aatttgctgc ttcagcacca ccttttgaat gtccgatcat
     2281 tgtgatttca atagtaggat ttacgtggac agccctcacc gtttaccctg accattatag
     2341 aaatcgctta cttttttgaa ctacgcaaca aaggatacaa tgctcctaac ttcactgtct
     2401 ccatttttgt ccactaagga atataaaaaa taggcattaa ttcatttaat tcggtcacaa
     2461 cacgttacct tttaaatcat tatactgtat ctcatacatt attatcaata aactattata
     2521 aaaatgcaaa aaatgcttta tcaatctatt ttgtaattgg tcataatatc attgaaatca
     2581 taccctgtat cgatttttcc tatgaatctc gtctatttgt gattgacgaa aaagaggcaa
     2641 gacagacact ggaatggtaa ttttacccac tattttgctc caactatttg taatcgtacc
     2701 cacttttgaa ataccgagat gatcatttta attgatatgc tgattcccaa gcaaaacccc
     2761 aagaaattaa atgctctatc gcatctagct tcttgggttt tgcttatata aattccaaca
     2821 tttaaatttt ttatgctgtt cggtctcaga aatgctgttt ttgcttatat cacaatatca
     2881 agatgtcaaa ttattcgtgt tgccgatgac tttgattaat ttttttcaaa ttgtttatta
     2941 agactaatgc tgtctttttg cagataccaa tgttcgaact ttttaacatt atcatatgat
     3001 cctgcaagtt ttaacttctt accattagat gttatcaaag ttataattaa ccttgccttt
     3061 ttcccattag gatcttttat tttatgaatt ttctgcaggt aatctctcct aatggcagct
     3121 atttttacct catcagtgct gataaaccat tgatcatcca catacatact gcctgttatg
     3181 ttgtgttttt ccatttcttc cgagaacgaa aagtttaagg ctcgttctta cttccaaagt
     3241 tgaatgagtc catggattgg acttagtgtg agaattgaga acaacaataa gaatccatat
     3301 cctcccgcaa acatccctaa ggctatactg tctctccatc cataagtagg attagaagag
     3361 aaaagagtca ccgtttgtaa taatatgaaa aggaataatc cagcacaaac accaaagata
     3421 acaactccaa acattgaatt tttatacaat gaccatttat ttacaggctt ttctttcatt
     3481 ttacttcccc ctactgttct gttcacacgg aatgaattca aagttcataa gccctcctct
     3541 ttgaacatat gaatacgact tgctaactat actttaaaaa agcttgtttt aagcggttat
     3601 aggcgagttg taatctctat tcatctcatt agcaaataac ttgccgaaaa gtctactgaa
     3661 attaaagcag atagtggaag aattatttag ccaggataga cactatttgt gaaacacatg
     3721 tctttacttt tgaaattcta tgatgaaaag ccttggaata cagtagctct taattggtaa
     3781 tactttttcc attataaata acctcttttc attttatatt tatcccctct aaaaataata
     3841 tctaatctaa tattttaaac ttatatttcc ctcgctgtgc taatagagca ttactaaata
     3901 aatagaaact agaaaaaagc attacgcctg ccagaataaa ccaattattt gtat
//
LOCUS       DAZKCZ010000136         3913 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000136 DAZKCZ010000000
VERSION     DAZKCZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.139"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..736)
                     /gene="panC"
                     /locus_tag="ACG8KN_002397"
     CDS             complement(<1..736)
                     /gene="panC"
                     /locus_tag="ACG8KN_002397"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP9189399.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVV"
     gene            complement(751..1578)
                     /gene="panB"
                     /locus_tag="ACG8KN_002398"
     CDS             complement(751..1578)
                     /gene="panB"
                     /locus_tag="ACG8KN_002398"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP9189400.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(1590..2450)
                     /locus_tag="ACG8KN_002399"
     CDS             complement(1590..2450)
                     /locus_tag="ACG8KN_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFP9189401.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            2688..3857
                     /locus_tag="ACG8KN_002400"
     CDS             2688..3857
                     /locus_tag="ACG8KN_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9189402.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRDRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT         1275 a          731 c          516 g         1391 t
ORIGIN      
        1 caacaacttc tacataatca atctttgcca aattataagt atttatattt ttacttatta
       61 tctcaattat ttttaagctg tctctctctc cattatttaa agcttcctta gctaaagtta
      121 aagatttatt taaaatagta gctgaagatc tttcctctaa acttaagtat gtgtttctag
      181 aacttttagc tagtccatcc tcttccctta taattggaca ccctacaacg tctatatcaa
      241 tattaaggtc cctaaccatt cttttaatta cagctaactg ttgtgcatcc ttttctccaa
      301 aataagctct atctgctggt attatgttaa ataatttact tacaacagtg caaacccctc
      361 taaaatgtat tggcctacta gctccacata aaccttctgt taatccagtt atattaacaa
      421 aagtacttgc attgtcacaa tacatctctt caacttcagg attaaatatt gctgttgctc
      481 tacctcttgt gcatacttct atatctcttt ctaagtctct tggataacta cctaaatctt
      541 catttttccc aaactgagtt ggatttacaa agatacttac aacaacttta tcattttctt
      601 ttgatgcttt tctaattaaa ctttcatgac cttcatgtaa atatcccata gtaggaacta
      661 atcctacctt taagccttgt tttttccact cttttacttg acttctaaca tcatttactg
      721 ttttaaatat aaccatattt cttgcccctt ttaatataat ttttctaaaa catcatcact
      781 tattttaaac ccatgttctg gacctggaaa aactccatct tttacttcct ctatatattt
      841 tgtaaatgct ttattcatct cttctgataa attagcatat ttcttcacaa atttaggggt
      901 aaaatcacta tacatcccca acatatcttg atatacaaga atttgtccat cacaccctgc
      961 acctgctcca ataccaattg taggtattga tatctcttta gaaattaact ctgcaagctt
     1021 agttggaaca cattctaaca ctacagcgaa tgctcctgct ttttctacag ctttagcagc
     1081 tcttattaat tctttagcag cttccttgtc ctttccttgt accttaaatc ctccaaatgc
     1141 attaacagat tgaggtgtta aacctatatg cgccataaca ggtatagaag cctttactat
     1201 agcctctatt ttatcgcata cttctatccc tccttctaat ttaacaacct gagctcgtcc
     1261 ttcttttatt aatcttcctg cattaactac tgaatcatat actgaagttt gataagacat
     1321 aaatggcata tctgcaacaa ctaaagtgtt cttagctcct cttgtaactg ctctagtatg
     1381 atgaatcatg tcttccattg ttactgaaag tgtatcttca tgtcctagac ataccatacc
     1441 aagagaatct cctactaata ttccatttat cccagcttca tcaattattt ttgctgttga
     1501 atagtcataa gctgtaagca ttgttaattt ttcatttctt aatttagatt ctttaaatgt
     1561 aactgctgta tttttcatct atttctctcc taactctctc ctaaatactt ttctaattca
     1621 ctataatctc tattaatatt ttttaacata gctatctcta atacattttt tgataatagc
     1681 atatataaag ctctgtcttc atctgataaa acttcacagt gacctttaat tgttgaaata
     1741 tcacaacgtt caactggccc tgtcaaactt tctataattc ctttttcttt tatatttcta
     1801 atattaaatt caattaatgg atataaagct tcaattgcca ttttttcagt aaatccacat
     1861 tgaattagat atgtaacgct tgtattaata agtcctagca caaggttact agatactact
     1921 gatgcggcat gataaagaga tttattctcc ttagatacaa ttacagtact attacctaac
     1981 tttttgaaga aatcacataa aaactttgca tatttttcat cgccttcaat tgttataaaa
     2041 gattttttta attttttgta tgaattatat ttatctgata ttgaaaacat aggatggaca
     2101 gaatagccat aagcaccata gtcctgtata tttgaaaaga tatctgaaga tattgcacca
     2161 ctgcaatggc agattaattt gtttttgatt tgataatttt taatttcttg ccatacctcc
     2221 tgaatttgac catcaggagt cgtaatgaaa atagtatcat tttcatctac taattctctt
     2281 aagtttaaat attgtttact agatgtaaaa ttcgaagctg ctaatgacga gtcttcgctt
     2341 ttactataaa atcctgttac ttgtatttta tgttcctgga aatacttccc gaatgaacaa
     2401 cctaccttgc cggctccaat aaaaccaacg ttcataatta tcacctccat aaagaagtga
     2461 gactacaagt cccattctta cgatatgagc ttaaaaataa aaattaaatc aaatatacag
     2521 tatggttttt tcaaatatca tccatagtta atttatcata atatcccata ttttaaaata
     2581 aaagatagcg taaaatattt tgtgtaaata gaaatttagg gtagaaaaaa aagtccattc
     2641 tgtaaaatta aagttaccac acagaaattt tataggagga ctttctcatg actaaattta
     2701 ctatagaaat tatgcaaaca ctactcaata aaggggattt agatgaatta tttcaggatc
     2761 atttagagcg agcgattaat tcacttctac aagcagagct aactgctttt ttagattacg
     2821 aaaaatatga tcgaaatgga ttcaattcag gaaattcccg taacgggaat tattcacgta
     2881 catttaaaac agaatatggc gagttgaatt taactattcc tagagatcgg aatggcgaat
     2941 tttctcaaca aacactacca gcatataagc gaaccaatga ttcattagaa acgaccatta
     3001 tccaattatt tcaaaaaggc attactatgg ctgaaatctc caaattaatt gaaaaaatgt
     3061 atggtcatca ctatacgcca caaacaatct ccaacatgag taaattggtg gctgaagatg
     3121 ttttagcttt taaagaaaga acgttagaag ccaattattc cgttatattt atggatgcga
     3181 cgcatatccc tgtgaagcga cagactgttt cgaaggaagc agtgtatatt acgattggta
     3241 ttcgtttgga tggaaccaaa gaagttcttg ggtttactat tgccccaact gagtctgctt
     3301 atatttggaa agaagttctt caagatctta gaaaacgtgg gttagaagaa gttttattag
     3361 tagtgacaga cggattaagc tgtattgaag aaagtatcca tagtgtgtat ccgaatgccc
     3421 aatttcaaca atgttgtgtg catgtatcta gaaatatcgc tcataaagtt cgtgttcgag
     3481 atcgaaaaga aatttgtgag gatttcaaat tggtttacca agcgaattca aaagaagagg
     3541 cattggatca catcgacttt atgactagga aatggaaaaa gcagtatcca agagtcgtca
     3601 atttactctt gaatcctgcc ctattaacct tttataattt ccctcacgcc atcagacgaa
     3661 caatttattc gacgaacctg attgaaggct ttaataagca gctaaaacga tatactcgaa
     3721 gaaaagaaca attccctaat gaagaatctc tagagagatt cttcatttct caatttaatc
     3781 aatataacca aaaattttta ggtagaattc acaagggatt taaagaaatt caggatacat
     3841 tagagtcgat gatttaactg taattaacag aatggatttt ccatttacac ataattcttg
     3901 acgctaccat ttt
//
LOCUS       DAZKCZ010000137         3874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000137 DAZKCZ010000000
VERSION     DAZKCZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.140"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..348)
                     /locus_tag="ACG8KN_002401"
     CDS             complement(<1..348)
                     /locus_tag="ACG8KN_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189403.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPF"
     gene            615..2315
                     /locus_tag="ACG8KN_002402"
     CDS             615..2315
                     /locus_tag="ACG8KN_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9189404.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2285..2503
                     /locus_tag="ACG8KN_002403"
     CDS             2285..2503
                     /locus_tag="ACG8KN_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFP9189405.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2523..3413
                     /locus_tag="ACG8KN_002404"
     CDS             2523..3413
                     /locus_tag="ACG8KN_002404"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9189406.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3431..3715
                     /locus_tag="ACG8KN_002405"
     CDS             3431..3715
                     /locus_tag="ACG8KN_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189407.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1352 a          643 c          793 g         1086 t
ORIGIN      
        1 aaacggcgaa tgaagatgtg ttttcatatg accaaaatat aataattctg aaatttctaa
       61 aggtgtcaat tctttcagca tcgttaaatc agtaaagtcg atccacttca attcatcgaa
      121 tccacgcctg ttacgagaag aggaaaagaa acgctgaata ttttctgtac cacggattac
      181 tttcagccct gtatgggatt catattctcc agcagaagca gatggatcaa gcaagagcaa
      241 gttttgagga aaatgcacga tactgtttgc aaagtcagta gcagtcagcc cttttgttaa
      301 aacagcatta cttgtggtat caatatgtac ataaactgac tcagtcatca tgttcctcct
      361 cttatttcct cttaatctct tttattttac aacatttgca ttaaaaaagc ttgtttcttt
      421 tattttaaaa ttccattaca aaatcacgtt ttcattgaaa acactctcat tttcaattaa
      481 tttttgtgat ttttgtattt ttttatttaa aggaggtttt atgaattgtt ttcttatgga
      541 atctacaaag tcgaatacga gactcatgac ctttttatga cgtactggta tacttgaaga
      601 aaaaggagag cgcgatggag ataaaaaaag tttacttttc acagaaaatg tcgctgtatt
      661 cgcctatcca gcaagcaaaa caaccccttc cattttttca aagtcatcaa gaaagtagag
      721 acgctttttg gcgttctgca aaaaagcaat gtgcttttaa agcagtatcg aaaaaatata
      781 ccgtctattt tactattgct aaaagtagag gagatgttat ttttaataat ctgttgattg
      841 aaggtgaaac ttttgaatgg aaaaaatttt ttcggtatat ggaagcttgc gctcgttttt
      901 ttattaaaga aaactgttgt tttttatttc aatctcctct atcagaagaa tggttgcgta
      961 tttttcataa aaacggctat caaggaacca cacaattaaa taaaaagctg gtctatcata
     1021 cagcacttgt tttaggtgga ggaggagcgc acggtgctta tcagattggt gtgtggcagg
     1081 cgctgaagga acatgatatc aactttgaga ttatcacagg tacctcggta ggtgcgttga
     1141 acggcgcatt gattttacaa ggagatatga aaaaagctgt gaatttatgg aaaaagctgt
     1201 cgacaagaca agtgcttgcg ttaccagaaa tggctgcagt tgaagacctt cgtgaacgct
     1261 tctatcgaga aagacgccaa atgacgaaaa cagcactaat tgaaggcggg gtttcttctg
     1321 ctccattgga aaagatggtc aaagacaatt tggatttgtc cttactcaaa cataatccca
     1381 aaatacgttt atatacggtg tctactaaac tgccagaact agcggaagta gtgactgata
     1441 tccaacagac aaaaacagaa gaaataccag attggatact cgcctctgct tcattttatc
     1501 ccgctatggc ttatcgaaag atcggaaaaa ataaatatgc agacggtggg tatcgcaaca
     1561 aaatacctat tgatattgcg attaacaaag gagcaacaga ggcatttgtc gtagatgtgc
     1621 aagggccagg cccagcgaaa aaaattcgta tgccagacat ttttatccat tggaaatgtc
     1681 aaacattgtg gacactaggc agctttctgc tttttgatag ccaaaggaac caattaaatt
     1741 tacaattagg ctatctggaa atgaaaaaac gattaggcta ttactatggg aactggtata
     1801 catttcattc tgttaaacct gcaagaacat actggcgagg atttttgtct tacttaacaa
     1861 aagaaattca gttagaatta tcttttttca aaagcataaa attttggcaa aaactgcgta
     1921 atttatataa gaacagggtc gtaccagaga catgcggatt ggcgatgcta gaacttttag
     1981 caaaaaaaca ttttttactg cccaatgaaa tctatcaagt agacatgatg attcaaatga
     2041 tctgccaaca agatataaaa agtatgccgg atgatttatt ggcacaaatt ggtcaattaa
     2101 gtactgaaga atggcgaagg tatcgaaaat accagcaaaa aatacaaaat gagcgcacga
     2161 aaactgttta ttttgattat ctgctacaag gaaaacgcaa ggatcgtttg cagcatgaat
     2221 tgcttaagca accagttgac acattactga tcctatattt atattatctc aaggaggaac
     2281 aaccatggca caagaatttt catatgaaat cgtagaagaa atcgctatat tgtcagaaaa
     2341 caacaagggg tggcgtaaag aactgaacct agtcagctgg aatggtcgcc cgccaaaatt
     2401 cgatcttcga gattgggcgc cagaccatga aaaaatgggg aaaggcctga cattgacgaa
     2461 cgaagagttt gaacaactgc aaaaagcaat agaaaatatg taatgaggaa ggaccctgtc
     2521 gaatgaaaag tatgactggt tttggcaaag ccatccgcga aacaaaagcg tatcagttag
     2581 atattgaaat aaaaagtgtc aaccagcgtt ttctagatgt tcaaatacga agtcctaaaa
     2641 tcctgaactt tgttgaaaac gattttcgtc agcttattaa acagcagtta agccgcggtc
     2701 gaatcgaagt atttgtgaat ctagcttata ttggcaaaaa cgaaaaacag atcgtcatca
     2761 actgggaatt gattgattca cttatcactc aattgacaga cggaattcaa caacgatacg
     2821 gtagtcaaaa tcaagtgtca attagtcgga tggcggaacg cttcgcgctg gacgaaaact
     2881 tcgtaacggt agaagaaaaa ccggaagaga gcatggaaga attggcacag ttagctctag
     2941 atacattgaa aagcgcactt gattctattg aggcgagtcg agaaaaagaa ggtcaagctt
     3001 tagctttagt gttgaaaaag aatactgaag aattcaaaga agtactaaaa aaactcgata
     3061 gttttgtaga aatctatgaa aaggaatttc aaataaggta tcaaaaaaag ctggaggaat
     3121 ttcttggagc gaaagtcgat gagcaacggt tgttgacgga gctagccatc ttgttggaac
     3181 gcggagatat tcacgaggaa ctggatcgtc tggcgatcca tgtacaaaaa ctagatgaac
     3241 ttatcgaagc acaatgtcct gtaggtcggg aattagattt cctgatccaa gaaatgaatc
     3301 gggaaatcaa cacgatcggt tccaaatcaa atgcaatcga aattaaaaat caagtcgtcc
     3361 agtctaagac gatcttagaa aaaatcagag aacaagtaca aaatattgaa tgacgaaaga
     3421 ggtagagata atgaaatatt atgacgtaac gttccacgaa ttaagtggaa aaactgtggt
     3481 taaacgggat atcccttctg aaaaaaacgg ctttgacgta tggaaagatg cctgtgcaga
     3541 ctataatgag aacgaactgt ttattttgat caacgacggt gcttacgtga cgatgaatcg
     3601 gaaatttatt gttcgaattg acacagaaga ggtagaagat ccgacagaaa aagcacgttc
     3661 tcgaaaagat gagatcatgg gtgtagtgaa tactttgtcc aatatgggct tttagcaagg
     3721 atgctgaaca aagaaataca ataaataatc agaattggat ttcttcgctt agcttctttg
     3781 ctctaggaat acatttaaag gttgtgacaa aattcgtgtc acaatctttt tttattttga
     3841 ataagtgatg ttcaaaaacc attttctcgg tatt
//
LOCUS       DAZKCZ010000138         3788 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000138 DAZKCZ010000000
VERSION     DAZKCZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.141"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..617
                     /locus_tag="ACG8KN_002406"
     CDS             <1..617
                     /locus_tag="ACG8KN_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HFP9189408.1"
                     /translation="ANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKYKAFRDFER
                     RVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQRDPAYLKG
                     AEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYPLYDRLSKD
                     RGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            652..1419
                     /locus_tag="ACG8KN_002407"
     CDS             652..1419
                     /locus_tag="ACG8KN_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9189409.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1611..2009
                     /locus_tag="ACG8KN_002408"
     CDS             1611..2009
                     /locus_tag="ACG8KN_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9189410.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2316..2690)
                     /locus_tag="ACG8KN_002409"
     CDS             complement(2316..2690)
                     /locus_tag="ACG8KN_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189411.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2712..3062)
                     /locus_tag="ACG8KN_002410"
     CDS             complement(2712..3062)
                     /locus_tag="ACG8KN_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189412.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            complement(3079..3474)
                     /locus_tag="ACG8KN_002411"
     CDS             complement(3079..3474)
                     /locus_tag="ACG8KN_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9189413.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(3541..3726)
                     /locus_tag="ACG8KN_002412"
     CDS             complement(3541..3726)
                     /locus_tag="ACG8KN_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP9189414.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
BASE COUNT         1283 a          569 c          667 g         1269 t
ORIGIN      
        1 aagcaaatag agataactta acatggagta caaaatcact tgatagatta aaaaatcctg
       61 ttttctccat tgcagattta cgaaagaaat ttagtattga gaaaaaatat aaagcttttc
      121 gagactttga aagacgtgta attaaagaac cgattgccca actgaataaa tacactgatt
      181 ttaaaattga ctatgagctt atcaagaagg gtcatactta tacaaacata aagttcttgc
      241 ttacacgtac catgtcagtt ccggtacctt ataagcaaga acaaagagac cctgcttatt
      301 taaaaggcgc agaagataaa acagagaaaa tgaatcaatc gtacttgaaa gctatgaata
      361 gcaaatacac taaattacta atcaaaaact ttttgatcag ttctgccgac ttatgcgatc
      421 ttaaacttat gagccgactt cagaataaag tttatccgct ttatgatcgt ttatccaaag
      481 atagaggatt tgaatcagtt gtcaatcacc tatcttacgt tgctactcat aaggaggatt
      541 acacaataaa aaatactgtt aaatatctcg aaaaatcaat tacgagttat ttgccttatg
      601 tgaaccttta tgattaacaa ctgaaaaaat gcgtgaagga aggaaaaact tttgaaaaaa
      661 gatgaactga caaagtacaa tgaggagttc ttagcaacaa ttgacatgaa aatggattta
      721 aagtcgtatg aactttatca attgctgttc agttatatct cacccacaaa gctaaagaaa
      781 acaggcgtag tagatttacc tgttaaagcg attattaatt acatggatgt tgatcaaaat
      841 cttgaaacgg aactaaaaac catagttaac actttgcaaa gacaagcagt ttatgaaatt
      901 tcgaatctag acaaaaaata taagacgtat actactgtat ctgctattga tcggattcat
      961 tgggatttag aagagcatag tgtatctttc agtttttcag aagatgtaat tcccttaatt
     1021 gtctcaatca aaaagaagta tccgttcttt gtttttgaag acttagcatc ttttcaaaaa
     1081 aaatatagca tcgtgttata caaatatttg gcgatgtatt ataaaaaata tcttatcgcc
     1141 aaaaataaag gagattttga gaaagctgaa atatataaga atcccctgat tgaaatagtt
     1201 aaaattcgtg agattacgaa aacagaaaat gagtataaaa caatatcgaa ttttgataga
     1261 cgtgttttaa gggaatcagt tgaggaaatt aatgaaaaat caatgctgaa tattgatttt
     1321 gctaaagtta gaaaaggcag aaattacaat tttgctgtcc agtttaatat ttctagtgag
     1381 tttgatgaaa aaacttatag agaaaaaaaa ttaggttaat gcaaaatata tcttactcgt
     1441 ttgaagatgc ttcagacgta tgctaagata tatagtgcct tgggagcaag gtattaatct
     1501 cctttagagg ttaattagct tgtgatcgcc tatcaatttt tttggtaggc gattcttttt
     1561 tttgaaacaa aaaagcaaaa attatttttt aattagagag gtgcaatgcg atgaaatact
     1621 ttttttgtgc agtattcgaa tggaaaggaa atgaatatgc agttaaattc ccagattttc
     1681 cagacttata tacacatggt gaaactttag aagatgcttt tgagatggcg caagaagtat
     1741 tggaaggata cttggtgctt ttagagttgc aagaaaaaga aattccatcg cctacgcctg
     1801 ttcaagagtt aaatgcaaga atcaagcgat ctgagaatgt taatttattt gtcgtaaaag
     1861 ctaatacgaa agttgctaaa gctcgaaaca agcaaaatta tgatcgaaaa tcagtgactg
     1921 tggaacacta tttaaatgtt ttagcggctg aagagaatat taatctgtcg aaagctttga
     1981 atgatttgtt gagagaaaag ttgatttgat gtctctcaga tgatcatatt aagcaaaaac
     2041 agcttgtttt aggacgtatg gatttaagaa agacaaatta tcaaagaata tattaaaagc
     2101 tctcagaagc cttctgagag cttttaaatt gctatgtatt gtactgcatc tgctcaaatt
     2161 gattttcttt tcatgcttac catgtatcta attttccatc aagtaatttt tagatatgta
     2221 ttgagtaata aatagcaaat ttgcttgata taagctcgaa ttaggtgctt tgtaatgtgt
     2281 taggataaaa ttatacaaga aaatgtcaaa agctctcaga acgcttctga gagcttttta
     2341 tcggtaggct tgtttaacgt gatcttgaag gatacccttt tgatgtttct ctcgatccct
     2401 gcatcgctaa ttactaagtg ttcttcaagt gtgatgtagg tatataaatt aagcatcaaa
     2461 atggtgtaga taagcaaaaa actccctaaa actaagtgtt gattataaaa atctgactta
     2521 taaaaaagaa ctgagaatgt tagtgttgat tcccaaaaaa gaaacaaaat tttgtatttc
     2581 caaagatagt ggatcaagcg ctgtgcttta cccatctcaa ataatttata ataacctgct
     2641 aattgatcag caagcgtatc gttaacggtt tctgtttgtg tttgtttcat ctttctcttc
     2701 tcctgtttca attaatctaa aatttcagat tgagcaagca actcattgtg gtagtcggtt
     2761 tgaaattcaa ataacaattt tgccttctta acgaggttta aagcgttcgt tagtgattca
     2821 cttaatgctt caacatcttt aactgatttt gcatgtttca ttttttcagt gtgcgtatag
     2881 cagaattcag gtattgagtt aggggttgcc tgttcaataa acatctcaat tcgcaagtta
     2941 ttatcctcaa aaataattag atcattggca aagtgtttgg tactaacgct aatgccagtt
     3001 gtatctttta atagattatt gattaacgtt cgagagaaat agtttaagtg tgtagctttc
     3061 ataggttcgg tctccttttt acttttcaag caactcttct tctagtgctt tttgaagtag
     3121 ttgagagaag tttatgtttt tctccttccc taaaagaact aaccattccg gaacactaac
     3181 attttttcta attgtttttg tatttaagct tcttcggaaa agtttaatgt ttagttggat
     3241 gattactacc acttggttgg ctttcaaagt taattcttcc actgctgatg gtttaggaaa
     3301 ttctgcatct ggtacactat atagcgctaa tcctaaagca tccttagcgt ttaaatatgc
     3361 ttcttcaaga ctatttccaa aggtagatat ttccggtaag tcagggaaaa cgatgttata
     3421 agcttctcct tctttctcga agattgccgg ataaaaaata ttttttgtct tcatttcctc
     3481 gctccttttc tagtgtaaga gaggatagta tgcctaatac caaataggaa ggcaaggctg
     3541 ttaaagccct gcttgcttcc agatattagc ttctgttcct agtggtacat caccattgtg
     3601 gatcggaaca acgaccgttc ttttagtctt ggaattataa aattttgcat gactgccagt
     3661 ttgtccagtc tttttgaagc cgttagattt gagaattttt acaatttctc ttgctgtctt
     3721 aggcatagct accctccctt tacaaaatct attatacaca ttgttgtgta tggaatcaat
     3781 agaaaaag
//
LOCUS       DAZKCZ010000139         3740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000139 DAZKCZ010000000
VERSION     DAZKCZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.142"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1108
                     /locus_tag="ACG8KN_002413"
     CDS             134..1108
                     /locus_tag="ACG8KN_002413"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9189415.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="ACG8KN_002414"
     CDS             1090..1551
                     /locus_tag="ACG8KN_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9189416.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="ACG8KN_002415"
     CDS             1601..2065
                     /locus_tag="ACG8KN_002415"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9189417.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="ACG8KN_002416"
     CDS             2079..2828
                     /locus_tag="ACG8KN_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9189418.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="ACG8KN_002417"
     CDS             2828..3658
                     /locus_tag="ACG8KN_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9189419.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT         1258 a          491 c          719 g         1272 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa ttttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtatcgtaaa gttatataac tttataatac ctattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtattaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgtatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttcataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgactcc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt
//
LOCUS       DAZKCZ010000140         3728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000140 DAZKCZ010000000
VERSION     DAZKCZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.143"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..214
                     /locus_tag="ACG8KN_002418"
     CDS             <1..214
                     /locus_tag="ACG8KN_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189420.1"
                     /translation="ASNESYGDLMDAYYSYLKKYAVVADSEKLLLYWPKYVQYNVKFE
                     NELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            240..1325
                     /locus_tag="ACG8KN_002419"
     CDS             240..1325
                     /locus_tag="ACG8KN_002419"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HFP9189421.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            1348..2163
                     /locus_tag="ACG8KN_002420"
     CDS             1348..2163
                     /locus_tag="ACG8KN_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189422.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            2147..>3728
                     /locus_tag="ACG8KN_002421"
     CDS             2147..>3728
                     /locus_tag="ACG8KN_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HFP9189423.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPV"
BASE COUNT         1255 a          492 c          741 g         1240 t
ORIGIN      
        1 tgcaagtaat gaatcttatg gtgacttaat ggatgcctat tatagttatt taaagaaata
       61 tgcagttgtt gctgattcag aaaaattgtt actatattgg ccgaaatatg ttcaatataa
      121 tgttaagttc gaaaatgaac ttgatttcta tgcgaagttg gattcaaaaa aatggatgga
      181 tatttatgct aattatacac cttttagtca gtgattatat ggaaaggatg aggtgaataa
      241 tgaaaatcgg aatagtgact attgttaatg gggcaagttt tggtaacaga ttacaaaatt
      301 atgcgactca aaaagtgttg gaaaataacg gctatacagt tgagacaatt aataattgtt
      361 ttgacaaaga aagtacccta tccattgtta tcaaaaacgc tatttataca ttatttaagt
      421 ttataccgca tttcaaaaag tcaaaaagta tctggaaaag aatgtataca ttttatcaat
      481 gggacaagca gtatatagat tattctcatc gtaaaattga tgcatctaaa aaaaacagct
      541 ttgtgagtat aaagaatgat tatgactatt tcttggttgg tagcgatcaa atttggaatc
      601 caaaatatgc agccaataag ggatttgaac ttttatcatt tgttccaaat gaaaaaagag
      661 tttcattatc aaccagtttg ggagttccat ctataccaag tgatttagaa aaattatatg
      721 tgacagagtg gaataagttt aaatcaatat cggtacgtga aaatcaagcg gcaaaaataa
      781 ttgaggactt gacaggcaca actgcggctg ttcacattga cccaacttta atgttggagc
      841 gttcggaatg ggaagccttt caaaaaaaac cagacaatat tgatgaaaat tacatcttgt
      901 cgtttttctt aactaatccg cctaaggata ctgttgatgt attagataag gtcgctaaag
      961 atttgaaaat aggtgttctc accttagatg cgtcttggga atattttcaa gatattactc
     1021 cgaatcattt tgtttatttg atttcacatg caagaatgat ttatacagat tcattccact
     1081 gcacagtttt ttcattgcta tttggaaaac cggttgtggt atttgaccgt tttgatagtg
     1141 aaaaaatgaa ttctaggtta agtacattgt taaataaagt aaataaagca catttttggg
     1201 taggaaaaaa agctgatgaa gcaatgattc gtgcaatagc atctgaacct gaatatgaca
     1261 tagataagct gatggtatca gagagggaat gtgtaaaagc ttatcttaaa gagtatttac
     1321 agtaaaattt tgaaatggga tgaaaatatg gagcatttaa gtgattgttt tggtaaaatg
     1381 aagattgtta cgacttcatg cgcaagagaa tattttaagg atgatttgga taaatcagag
     1441 tattcatgtt ttgttgattc tcgagcagct atgtttttcg caatgggaca agctttagct
     1501 aaaagaagga atgtagttgt aatgcttgct gatgaattga catcgtgtta tacggcacta
     1561 acagaatgtg catttcaaca agttgattta attgtgtttg cttttcagac agtcagacat
     1621 gaaattgatt tagcatcttt gaagaattgt ggttgcaatc taattaactg tagcgcccaa
     1681 gacgatattg atagagttgt gtccaacact aaggggttaa atcagacaac agtaattaaa
     1741 atcaatatga attgtgagtc ggataaagat catatatgtg agtatataga cctgccagac
     1801 atacctgttt ttttccacgg tagcttatac tcacctgcaa gtaaaaagaa atatgagcag
     1861 tatggacagg tatcgaagta tttaggctat ctcgtggggg gaaaagatgc aatactcata
     1921 atttctcaca atgagttttt aaacgatatc agtgtatgga ttaattatta ttcttttgga
     1981 gaaattcaac ccaaaattat tatatttgac agtccgaatg actatcaata taaatggctg
     2041 tgtgacttag aatgcagtgt agctcattca accaaccgat ttttgtctga ggatttagcc
     2101 ttattgcaaa ataacggaat ttccattgtg ttaagaggtg ataattatga tatcgtcagt
     2161 taaaaatgtt caaattttag tatctttatt aaaacagcat aatattaaag atatagttat
     2221 ctcgccaggt agtagagatt ttcaattggt gcatagtgta gaaacagaca gtttttttag
     2281 aacctatacg gttgtagatg agcgcagtgc tgcctttttt gctaatggac tttctatggc
     2341 gattgataag cccgtgtgtg tatgttgtac ttcttcaact gcctcttcaa attatatggc
     2401 ggcagctaaa gatgcttttg ctaatggcat ccaacttgta cttcttactg ctgatagaga
     2461 ttaccgccgc ttatatcaga tggaagatca gatgattgat caggtagata tgtatggggc
     2521 ttatgtgcgt tctaacgttg atattcctat tgttaaggat tctgatgatg aatggtatgc
     2581 taccagaaaa ataaatgaag caatattgga gttaaatcat ggcgttaagg gtcccgtgca
     2641 gattaattat caattaatgg atttaagtgc gccgtttgtt gagaccatgc cagagtatcg
     2701 taaaattaca agatatcaag aggatgattt actgtcatca gtagagattt ttaaacagaa
     2761 actattatca aaaaagagaa taatggtttt atgtggacag caggaagaaa ataatttaag
     2821 tgccttattg gaagagttct ttattaaata taatacaatt ataatcaagg ataactattc
     2881 taatatacac ggtgaacact ttattaagac tgttctagta acagaaagaa tgagtgtaaa
     2941 tgcatttatt gattattgcc cagatttggt aataactatt ggtaaacatg tatggtcttt
     3001 tgtgaaatat actcttagag ataaaaagct taaatttgag cattggcgga tatctgagga
     3061 tggtaagatt gttgatccgt tcaacaagct aacatctgtc tttcagtgct cagcggaatt
     3121 ttttatggag actgtcactg ctggtgtgaa tagcaataat aatcttgagt attataatct
     3181 atggaagaca agattatcaa aagtttgcta tccagagtta gtctatagca atttttatgc
     3241 aatttcagaa ctagtaaaaa aaattccaga tagtgcatta gttcattcta gtattttaaa
     3301 tgcggctaga ttgtatgact ttgtatctga tgataaagca ttactcagtt ttgctaattt
     3361 aggggctgat ggtattgatg gttgtttacc aacattttta ggtcaggcag aagcagataa
     3421 aactcgacct gcgtatttaa tttcaggaga cttgagtttc ttttatgcta tgaatagttt
     3481 atgtggtgac attccagata atgcacatat tttccttatt aacaatcatt ctggaagtga
     3541 attccataca aattttggta agttttacaa cggatcatta agtgttgata agcatatagc
     3601 tgcaggacat atttcaaacg caaaggattg ggcattagaa aataatataa aatacattac
     3661 tgcggattct aaggaaagtc tcaaatcggg gttggatgta tttattaata catttgacag
     3721 tcctgttt
//
LOCUS       DAZKCZ010000141         3726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000141 DAZKCZ010000000
VERSION     DAZKCZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.144"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            261..1754
                     /locus_tag="ACG8KN_002422"
     CDS             261..1754
                     /locus_tag="ACG8KN_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9189424.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            1889..2185
                     /locus_tag="ACG8KN_002423"
     CDS             1889..2185
                     /locus_tag="ACG8KN_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFP9189425.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2286..3137
                     /locus_tag="ACG8KN_002424"
     CDS             2286..3137
                     /locus_tag="ACG8KN_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189426.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3158..3370
                     /locus_tag="ACG8KN_002425"
     CDS             3158..3370
                     /locus_tag="ACG8KN_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189427.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1305 a          549 c          767 g         1105 t
ORIGIN      
        1 gaatcacatg gttaggacat gtgttcacgc tcgtctcaag aatatgtaag tagctaccaa
       61 ctacttatat taaaagttga tcggatacct tttccgattg cctttattct accaaaaaaa
      121 ggcgagcaac tcaacagaag aacgtttatt tagtgtactt aaattaaata ttcttaaaca
      181 ctgaagagag aacacatact atggctgaga ttaaagctaa actgtgttct ctttttgttt
      241 gagaaaggac gttacaatcg atgttgagcg aaatttattc agctattctt aaaaacggct
      301 tacgtacata caaatatcga aacagcacca tgaaacctgc aggtcatatt gattccagta
      361 ggagtggtgc tatctttgcg taccgtagca agcaattaat gaatgttggg cgtggtatgg
      421 tcattacttc ggaagaagct attttagaga acgaaaagaa actaacgcat tggacaccta
      481 atacctatcg ctttggtact tatgcggatg attatcgaac agtggttaaa ggtcattctg
      541 agaaaaactt aagtcaaatc aacacatttg tagttgatat cgattcaaaa gagaaccatc
      601 aaggcgaaat tattttagcc tgtttagatc aagtgggcta catgccaacg ctaattttag
      661 aatcagatca tggttatcaa gtttattttg tactaaagac accaggctat gtcacaaaga
      721 aaagcaattt caaggtgatc gatgtagcaa aaaaaatttc aaaaacaatt cgtctacagt
      781 tggctgaaaa attgtccgga gtggatctag gatgtaacca cttcggaatt gctcgattcc
      841 cacaagaaaa taatattgtt tttaaagaaa tgaactatca gtattctttt gctgactgga
      901 ttcaatggtc gatgaagctt gaatcagatc aaaaaacaaa agaagatcga gaacgaaaac
      961 taattgtctt tcctgagaaa aaagaaattc gtcaagtaga tgaaccgtgg ttcgatatgt
     1021 tacttcataa agcaaacatc gtcggcggag aaggacgtct tggtcgaaat aatgtgattt
     1081 tcacgctttc tttagcttat tactcttcag gttatgcatt ggatacttgc gaatacaata
     1141 tgttcacatt caacgaacgg ttacaggaac cgctatctga agcagaatta gtaaagattg
     1201 tacgtagtgc ttattctggt aattatcaag cagcaagcag agaattcatt attgaattgt
     1261 gccagcaatg ggtttcacca accttaaaaa ataatgatct gttcattcaa cgacgtggct
     1321 ggtggaaatt taaaaagcca cgagatcaaa gaaaatatag tcataaaaat gaatggaaag
     1381 aagatctatt ggcttatcta agcgaaaaaa gtcttgctag tgagccttat ttgaacctgt
     1441 caaaaaaaga attggcttca caactaacga tgcccaagcg aacacttgat aagatcttaa
     1501 atgagttacg agaggagaat aaagtcttct acaaggtaac acgaggaaga aatggcggaa
     1561 tgatcattgc ttcagtcaaa acgttgttcg cgagggttat tcaactaagt aaagaaaaga
     1621 gactagatta ttcaacaagg atcacagagg tatttggact caacgagagc ttagtagaag
     1681 agacatttag acaattatca acccccttga accctcttaa agaagtgaat ctttttgaag
     1741 tagatacagg ttgaaaaata aaaggtgcaa attgccctat acaatatatc tatgatactt
     1801 ctgtaggtgt tgctcccttt gcctagctac gtgtaggtgg tatacttata actgcgtatg
     1861 cagtcttatg tatcatggag gaagttgtat gtcgttaaaa aattatgtct attcacatcc
     1921 tgttaatgtg tttatcattg gtcgtttggg attatctgtg gaagtgttct gtgagcttta
     1981 tggttttaag caggggactt tgtcttcttg ggttaccagg gaaaaaacag ttgcttcttt
     2041 gccgattgag tttcttcatg ggttgtcctt agcttctggg gagactatgg atcaggtata
     2101 tgattgtttg tgtgtgttag agcaggagta tattgagtat aaaatagcta acgagttaag
     2161 aaaaaggaag aagtatatcc agtagtagtt tttatttact taaaaaattg gttcattgag
     2221 caacctatcg ctgctaaagt agcaaaggaa aacgttaaag gaaagaattt agggaagaaa
     2281 tcgaaatgaa atcggacgac tggattgaac acacctggct tcctctgaaa agaaatagca
     2341 aacgaaagaa attttgggta ttgcaattat ggccattagg aaaactttgt agttacgggg
     2401 cacgacttat tcttgattta accgtttact cgattggagt atttggaacg gcttattact
     2461 tgataaatga tcccaatagg attgcagcat cagacatttt tacagtgcta atgacgttga
     2521 tttttgtcat gctattgtct atttttactc aattagtcat gcaatttacc aggcaaaaat
     2581 taacagcagg aaaacttttc aaaagcctat tcgatttaat tgttgtaaat gcaattctag
     2641 cgactttaat aaaacaaatt gaacaatctg gaagaacaac aaaaggagtg aaggattttt
     2701 tattgacgtt tattcacttt gtaaatggac acatgtattt gtttgaagtg ggtattattc
     2761 tcttttttgt cgggtatgta tccacgaaac ttattaaacg ttcaattgaa aaagaaattg
     2821 aaattgacct gattccgaca cttcttggtt atgagtacaa tgttgatttt ggaagatatg
     2881 acgaattgat caatacggat atcgttcaca cgagtatcca tgaatctact gttaagggca
     2941 tcagatctat tttaaaaaat ggccaggaaa tgaaagcaat agatcgaaga aaagagatct
     3001 acaattttta tgaagtcgaa gcaagcctac gatatggcat cggaaaattg tggttagatg
     3061 gaagtaatat tcgtcaaaac aaaccataca ttttttggaa cagtagtgat ttagaacttc
     3121 caatgaaaga taaataatta tggagaatcg tttgaaaatg attcctctat acaggattgt
     3181 gcttatagtt ggcatggagt taaaaacctt aaagaaggag cagttattca aagtgaaaga
     3241 acgtcataat cagttactat gtagtaaaac gggtacgatg aacattgaat accgaacaga
     3301 atatttagaa ttaacgaatc aaactgattt tttgttgatt cgaaagcaac aagagcaaga
     3361 aaaaaattaa tggctactta tagccaattg attgatgaat gggaacaatt caaggaatct
     3421 aaaattccag aacgtctcta aaagccttta tttgtaaaga gcataaggaa acgtgggaac
     3481 actttttaaa aaaagtgttc caataggctg tgcctattac gtttctcaaa aataaatgtg
     3541 ggaacaaaat atttaaaaat aggatagtga aaggatagtg aaggatagta aaaggagggt
     3601 agtaggatag caaaaaggat aggctccttt tcgctatcct ttttaaattg gaggaattaa
     3661 tatggaagta agaataagaa atgtggatga gaaaatcgta agagaaattg atcgaagatg
     3721 tgacga
//
LOCUS       DAZKCZ010000142         3712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000142 DAZKCZ010000000
VERSION     DAZKCZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.145"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..1737
                     /gene="efrA"
                     /locus_tag="ACG8KN_002426"
     CDS             7..1737
                     /gene="efrA"
                     /locus_tag="ACG8KN_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFP9189428.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            1734..3494
                     /gene="efrB"
                     /locus_tag="ACG8KN_002427"
     CDS             1734..3494
                     /gene="efrB"
                     /locus_tag="ACG8KN_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFP9189429.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         1140 a          615 c          826 g         1131 t
ORIGIN      
        1 gagaaagtga aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat
       61 ttcatatgcg tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt
      121 atgatcgatg taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt
      181 atgatcgtta ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg
      241 aggattacga ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt
      301 tattcgcatc aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac
      361 gatgcttatc agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca
      421 atgatgttca ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat
      481 gtagtaggtg cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa
      541 ccgttatcta aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat
      601 ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt
      661 gctgtagtca atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt
      721 gcacaaccgg gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg
      781 gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat
      841 atattccatg ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga
      901 gcagctgttt cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa
      961 gatccaaatg gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca
     1021 tttgcttatc caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct
     1081 ccaggagaga ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa
     1141 ttgatccctc gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg
     1201 agagattatc ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg
     1261 ctatttactg gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa
     1321 gaaatggaac gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat
     1381 ggatttaatg aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga
     1441 ttggcaatag ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt
     1501 tctgcattag attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca
     1561 gaatctactg tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc
     1621 atcgtattaa atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa
     1681 tgcccgattt attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa
     1741 aaaccttttc ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc
     1801 ttttagtgat cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta
     1861 caggacttcc tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt
     1921 cttatatatt caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc
     1981 aattactttc tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa
     2041 gagacataga agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag
     2101 gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag
     2161 cttttattgg tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt
     2221 atatccagcc gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct
     2281 caaaacgagt gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg
     2341 agctaaacgg ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac
     2401 gcgaaaaaga aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt
     2461 ttcgtgcagc attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta
     2521 cttatattgg tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg
     2581 gtcaactaca agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga
     2641 cacaattgtc ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt
     2701 tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag
     2761 gagatgtgac attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag
     2821 atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg
     2881 gcaagacgac attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa
     2941 ggattgatgg aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta
     3001 tggtcttgca agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa
     3061 agcttgatgc gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt
     3121 ttatccgaac gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt
     3181 ctttaggaca aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct
     3241 tgattttaga tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag
     3301 caatggatcg cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa
     3361 tccgtgatgc agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc
     3421 atgagagttt attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg
     3481 aggaagcaga ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat
     3541 tcttgataat ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt
     3601 ttaaatgaaa aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag
     3661 acgaagcctt ttgattcagc tgattttttt gaaaatgagt ttcaaatcga ac
//
LOCUS       DAZKCZ010000143         3597 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000143 DAZKCZ010000000
VERSION     DAZKCZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.146"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..949
                     /locus_tag="ACG8KN_002428"
     CDS             59..949
                     /locus_tag="ACG8KN_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFP9189430.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1022..1846)
                     /gene="nadE"
                     /locus_tag="ACG8KN_002429"
     CDS             complement(1022..1846)
                     /gene="nadE"
                     /locus_tag="ACG8KN_002429"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9189431.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1858..3330)
                     /locus_tag="ACG8KN_002430"
     CDS             complement(1858..3330)
                     /locus_tag="ACG8KN_002430"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9189432.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         1032 a          744 c          636 g         1185 t
ORIGIN      
        1 taacttttat taacaaatat gttaatatat aagaaaaacc ttttaagagg tgatatatat
       61 gcaactacaa ataaacgaag aatccttacc ggtatatgaa gcattagcta gtaaaacaag
      121 gatcaagatc atccagctac tttccaaaaa aaagatgaat gtcaaagatt tggcaaaaga
      181 acttggagtg agcagtgcta tcacgacgat gcacgtgaaa aagctagagg aagcaaacat
      241 catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg ataaaatcga
      301 tatcagtttt ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat cgatacctat
      361 tggtcactat acaaattatg caattgaacc aacatgcggt ttagctacga tccatgattt
      421 tatcgggaaa gtggatgaac cacgctattt tatggatcct cgaaggatgg atgcccgcat
      481 tctctggttt accagcggat ttgtcgagta tcaggcacct aattttttaa atacagagga
      541 tacattggag atgttggaag tttctgtgga aatttcttct gaatttcctt tttcaaatga
      601 caattggcct tcagatatca ctttcagcct aaatggtgtg gagctaggaa catggacgag
      661 ccctggagat tttgctgata tccgcggtaa atatacgcct gattggtatc cagataatct
      721 taatcaatac ggcttgctga agacgatccg aattaccaaa cacttgacga atatgaatgg
      781 agaacccttg tcaaacatca cgatcaacga catccctaaa gagcaagaca cttggcattt
      841 gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc tgtactttat ttggtaaagg
      901 attcggtaat tacgatcagg atatcaaatt aaaagtttac tacagctgat atttttcttt
      961 tctattttat aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt ttgctttatt
     1021 gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt tgtgtcttgt
     1081 tccaccatgc ttcgattttt tcttgtgctt gagcgggaac ttcttttcct tctagataat
     1141 catcaatttc attatatgtg acacctagtg cgacttcatc tgcaatcatt ggtttcccat
     1201 cttcaagatc tgcagtcgga atttttgtgt ataatttttc cggtgcattc aatgcttgaa
     1261 gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg gctccaccat
     1321 caccaaattt cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc cctaatacgg
     1381 ctcctgctct ttctccagct acggcatatt gcgtaatcat acgttgacgt gctttgatat
     1441 ttcctttgtt aaaatcgctg attcccacac ctgcttcgct taatacttgt acttgtgcat
     1501 caacagctgg tttgatattt actcgcagac taacatcggg ctggataaag tcaagagcag
     1561 cttttgcatc ttcttcatct gcttgttcac cataaggaag acgaacagcg ataaattgat
     1621 agttttcatc ttttgtttct tcacgcattt cttccatcgt tagttgcgcc aaacggcctg
     1681 ctaaagtaga atcttggccg ccgctgatac ctagtacaaa agttttaaga aacggatatt
     1741 ttttcaaata tgctttcata aagtcaatac ttttacgtat ttcttctttt ggatcaattg
     1801 ttggtttgac acctaattca ttgataattt ctgtctgcag gctcatttat tctcctccta
     1861 tgcttctgta tgaagttctg ctacgctttt tttcattcgt tccatgatag ccattttatg
     1921 gttccagcat tcagtagaca gatcgactgg gtatttttgc ggattcaagt ctcgtttata
     1981 ctcttcccat agtgaatcta gattttcttt tgtatattct ttgatttcgt ttagtgcagg
     2041 taattcgtaa actctttttc cttcgataaa gatatcttgt aaaactgggc gagctgtaaa
     2101 gtctcgaaca gttttattga taaacgtatg cactggatgg aacatgaaga tagcttcttc
     2161 ttctcgaggg tcttcatccc ataatgtcac gtagtctcct tcagatttcc catcaaaatt
     2221 tcgagtgatg cgccacactt gttttttccc tggagtagtt actttttctg cattgcttga
     2281 taacttgatc gtatccatca tttttccttc atcatcttcg atcgatacta gcttgaatac
     2341 tgcaccaagg gctggctgat cataggctgt gatcaatttt gtcccaacac cccatacatc
     2401 aattttagct ttttgcatct ttaaattcaa aatcgttgct tcatccaagt cattcgaagc
     2461 atagattttt gcttccgtaa atccggctgc atctaattgt tcccgaacac gcttagatat
     2521 ataggccata tctccactgt cgatgcgtac gccttggaag ttgattttgt cacctaattc
     2581 tttcgccacg cgaatcgcac ttggcacacc tgatttcaac gtatcataag tgtcgactaa
     2641 gaacacgcag tctttatggg ttttagcgta ggccataaat gcttcataat catttccata
     2701 agattggact aacgaatgag catgggtccc gctagctggt atcccaaata tcttcccagc
     2761 acgtacattg cttgttgcat ctgctccgcc gatatatgcc gctctagttc cccagactgc
     2821 tgcatccagt tcttgtgccc gtctagttcc aaattccaat agtggatctt ctccgatgac
     2881 agatttgata cgagctgctt ttgtcgcgat caacgtttgg aaattgacca tattcaacag
     2941 tgctgtttct actaattggc agtgcgctaa aggcccttcc acttggatta atggttcatt
     3001 tgcaaaaact agttctcctt cacgagcaga acgaactgtt gctttaaatt caaaattttg
     3061 taaatactcc aaaaaacctt caggatagac ttctaatcca cgcaagtaag caatatcgga
     3121 atcattgaac gtcagattct ctaaataatt gactaaccgt tccaatcctg caaaaaccgc
     3181 gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca aaaacagcat gtaaatcagc
     3241 tcgacctaat tcccagtatg tctgcatcat attgatttga tatagatccg tatgcagcgt
     3301 caaactgtcg tctgggtaag tattattcat cttgtattac tcctcttggc aagttttcat
     3361 ttcctaccca ttataacaaa aaaaattaat aggaaaagaa atcttccatt ttttctgaaa
     3421 aagcacatgt tttacggata tttcttgcta aaaatgaact ccatctgcta aaactatcct
     3481 ttcttattgg tatttaacct aaacgagaaa gaatacttat tttattcaat gtatccgaga
     3541 cacgaagaaa ttagcgtttt ctatttttct tacttgataa aataagtaga ttttcct
//
LOCUS       DAZKCZ010000144         3594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000144 DAZKCZ010000000
VERSION     DAZKCZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.147"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..276)
                     /locus_tag="ACG8KN_002431"
     CDS             complement(<1..276)
                     /locus_tag="ACG8KN_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189433.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMP"
     gene            complement(364..774)
                     /locus_tag="ACG8KN_002432"
     CDS             complement(364..774)
                     /locus_tag="ACG8KN_002432"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9189434.1"
                     /translation="MISFEKAFSIFAIVLMYYFQQSYLPAILNKAISIGDTGVHIFFF
                     CSGFGLYLSYLKKPITLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSH
                     VLLFKIFLPVYEESLGPFGLFRQLFSSISYSYQL"
     gene            complement(1073..2725)
                     /locus_tag="ACG8KN_002433"
     CDS             complement(1073..2725)
                     /locus_tag="ACG8KN_002433"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFP9189435.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            complement(2785..2925)
                     /locus_tag="ACG8KN_002434"
     CDS             complement(2785..2925)
                     /locus_tag="ACG8KN_002434"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9189436.1"
                     /translation="MIKFVFLCSCLIEFLQLFLHIETFQLSDLFYNTLGGGISGVIYY
                     LS"
     gene            complement(2919..3275)
                     /locus_tag="ACG8KN_002435"
     CDS             complement(2919..3275)
                     /locus_tag="ACG8KN_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189437.1"
                     /translation="MFLYLFAMAEEKSGLGLKKSFLVWIRYFRKSSSFRKMFLLVFFT
                     NMILFRTLMDRYVWLNPLSNVFGSWWIYSVNETTGEIRLTTECFENVILFIPFIILVM
                     WYKASEKDFGKIYQRL"
BASE COUNT         1165 a          837 c          583 g         1009 t
ORIGIN      
        1 aggcattatc tttctaaatg gaataacttg atacattaaa attgcaattg caccgtaacc
       61 gaagaatgca aaaatatcat taatagactc catcagtcca ccaataattt tcgatatacc
      121 agcaaaacca attccaacaa tggacgccat aatcacgaat atattattaa atcttactat
      181 ttttcttttc actaaatact ctgcaaccaa acatgccgaa agtaaattcc caaatatatt
      241 gcgcaaagca gctcccaaga actcgtatgc ttccatatcc tgtatgaaac atcataatag
      301 cccagatact aataactcag cttgcaacaa gcaatcattt cattcctatt tttctctata
      361 gttttacaat tggtatgaat aagaaataga actgaataat tgtcgaaata aaccaaaggg
      421 acccagtgac tcctcatata ctggtagaaa aattttaaat aacaaaacat gagacaaata
      481 tacttttgca gcatttaaat cttgtgtcat ataaggtagt aacactgata ccgtaattac
      541 gataatatac ggaatataaa tcttgataaa tcgtttctgt aaaaattcac ccaatgtaat
      601 aggctttttt aaatatgaca gatatagccc aaaacctgag caaaagaaga atatatgcac
      661 tcctgtatca ccaatagata ttgctttgtt cagtattgct ggcaaatacg attgctgaaa
      721 atagtacatc aatacaatag cgaatattga aaacgccttc tcaaaactaa taatatcacg
      781 tttatttctt tccccctcat tttattattt caatagtttt attcatattc tactcctttc
      841 atctctattg ctgaatacat tattaaaact ctaatatctc ttatttctcc cttactgctt
      901 tgatcggtgc ttcatcaggc tgctgtgtct ctgttaacac ttctttgcct tttcgatcca
      961 cacagtcggc ccctttacct caattatgta aacagaaccg taaagcagga aatttttatt
     1021 aaacatagat gttgctactt cggacattga cactcagatc tttttcatag tattacttaa
     1081 acatttcagc cagttcagca gccataacag cttccattac ttcggcagct tccttgcttt
     1141 ctgcgctaac gctgatatag gtcttcagct tcggctcagt gccggaaggc cgaaccacta
     1201 cagagcagtt gccctccagc agatatttga gaacatcaga cttcggcagg ccattcagtc
     1261 ccgtgctgta atccaaggtt tcaatgacct tcttacctcc aatcatatca agacctttat
     1321 ggaattcaac cataattttc tgcatcttgg taaaacctgc cgcaccctca aactcaaagg
     1381 aatgcagggt gttcaaacag tagccatact tagcataaat ctcgttcagc ttatcaaaca
     1441 gactgatacc ctgagtcttg tagtaagaga acatctcgca gatcagataa gcggcgttga
     1501 caccatcctt gtcacggaca tagctgccgg atagatagcc gtaggattcc tcaaagccga
     1561 aaatatagct gtccaccttg cctgcctttt ccagcatacc aatctgttcg ccgatgaact
     1621 tgaagccggt caacacatcg acagtacgca cgccatagtt ggaagcgatc cgctcgccca
     1681 gatccatagt cacgatggtc ttgaccatca cagggtcagt gggcatcttg ccgtgcttaa
     1741 ttctctgaga gcatatgtag tccagaagca acagaccagt ttcgttaccg gacagcagga
     1801 cataatcccc ttctgcattc ttcacggcga tacccacacg gtcgcagtca gggtcggtag
     1861 ccagcagcag atcagcatta tacttcttgg catactccat gcccagagac atagcttcat
     1921 taatttccgg attcggataa ggacaggtcg ggaaattacc gtctggcaga cgctgctctt
     1981 cgacaacggt cacattgctg tagcccattt ctgccagagt acgggtaaca ggcaccagac
     2041 cggtaccatt caaaggagaa tacacgatag caacattctt gtccacttct tcgccaaaca
     2101 gaaccgactg gccctttact tcttcaatga aagcagtcag aacctctggc tggatagaag
     2161 cgattctgga agcgtcgccg cccattttca catctgcaaa aatatccagc ttttcaattt
     2221 cagccagaat ctcagcggca gcctcggtgg tgatctggca gccgcctgcg ccatagacct
     2281 tatatccgtt atacttggaa ggattatggg atgcggtgat cataacaccg gcagaagcct
     2341 tcagataacg gatagcaaag gatacggtag gcacaggcag cagctccggc cacagatgca
     2401 cctgaatacc gtttgcagca aagatgtccg cggcaacctt tgcaaataca tcggacttga
     2461 ttcgggaatc atagcccacg acgacagaag gggtttcata ggtcttattc agatagtctg
     2521 acagtccctg gcttgcctta cctacggtgt gtacgttcat acgattagtg cctgcaccaa
     2581 tggtgccacg cagaccaccg gtaccaaagg acagattttt atagaaggag tcctcaatgt
     2641 ccttctcgga atatgaccgc agttcctcta caatttctgc attggcattt gtaagccaat
     2701 gataataatt tgcattatga tccatagaat ctctccaatc ttttaaaatt ctgctcattt
     2761 atttttaagg ttaggatgaa tatatcagct caaataatat ataaccccac taatgccgcc
     2821 acccaaagta ttataaaata aatctgacaa ttggaatgtc tctatgtgta aaaataactg
     2881 caaaaactct ataagacacg aacataaaaa aacaaatttt ataatctctg ataaattttt
     2941 ccaaaatctt tttctgaagc cttataccac attactagaa ttataaatgg aataaacaga
     3001 ataacatttt caaaacattc tgtagttaat ctaatctccc ctgtcgtttc attcacagag
     3061 taaatccacc aactaccaaa cacattagac aaaggattta gccaaacata tctatccatc
     3121 agtgttctaa aaagaatcat atttgtaaaa aacacaagca aaaacatttt tctaaatgag
     3181 cttgattttc tgaaataccg aatccagaca agaaaagatt ttttcaaacc taatcctgat
     3241 ttctcctcag ccatcgcaaa caagtataaa aacatagcca aaactgcaat taaaatcgaa
     3301 acccaaatat ttgatacaat gcagataaca cattaaccaa aatagaagat atgattttct
     3361 tcataaactc tcctttcatc atgcgctaaa tgagtaccat caagaaggtt tcgtaaaagg
     3421 aggtacccac tctaacatga ataacctcgg ccagcgaaaa taaaactcaa cagaattcct
     3481 atctctatgc tgctataatt tcaacaaatt tacatgtacc agttactaat ctaatataat
     3541 tttttcagca aggtaaattt cacactacac gcatacacaa aaactgtaga aatt
//
LOCUS       DAZKCZ010000145         3591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000145 DAZKCZ010000000
VERSION     DAZKCZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.148"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..1046
                     /gene="galE"
                     /locus_tag="ACG8KN_002436"
     CDS             57..1046
                     /gene="galE"
                     /locus_tag="ACG8KN_002436"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9189438.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1058..2542
                     /gene="galT"
                     /locus_tag="ACG8KN_002437"
     CDS             1058..2542
                     /gene="galT"
                     /locus_tag="ACG8KN_002437"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFP9189439.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2566..3546
                     /locus_tag="ACG8KN_002438"
     CDS             2566..3546
                     /locus_tag="ACG8KN_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9189440.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1218 a          658 c          790 g          925 t
ORIGIN      
        1 ttttaaatta tatagaatca acaaacacaa ttttactaat caaaggagtg ttttttatga
       61 caattttagt actaggcgga gccggttata tcggttcgca cgctgtagac cagctagttc
      121 aaaaggggta tcaagtagct gtggtagata atttgctcac aggtcacaaa caagcagtac
      181 atccagatgc tcatttttat gaaggagaca tccgggataa agaatttcta cgttccgtat
      241 ttgaaaagga accgattgaa ggagtaatcc attttgctgc aagctcttta gtaggagaat
      301 ctgtcgaaaa accattgatg tatttcaaca acaacgtata cggtatgcaa atcttacttg
      361 aagtcatgca cgagttcaac gtgaataaaa tcgtattctc ttctactgca gcaacttatg
      421 gagagccgaa agagtctcca atcacagaag atacaccagc taatcctaaa aatccgtatg
      481 gtgaaagtaa attgatgatg gaaaaaatga tgaagtggtg tgaccaagca tatggcatgc
      541 gctatgtcgc tttgcgctac ttcaatgtag ccggagcaaa agcagatgca tctatcggag
      601 aagatcatac accagaaacg catcttgtcc caatcatttt acaagtagct cttggtcaaa
      661 gaaaagcctt ggctgtttat ggagacgatt atgatacacc agatggaaca tgtatcagag
      721 attatgttca agtagaagat ttgattgctg cacatatttt agctttggaa tacttgaaag
      781 aaggaaatga aagcaacttc ttcaatttag gcagcagcaa aggatactca gtcaaggaaa
      841 tgctagaagc agctagagaa gtgacaggaa aagaaattcc agcagaaata gcaccaagaa
      901 gagctggaga tcctagccga ttagtcgctt caagtgaaaa agcgagagag attctgggct
      961 ggaaacctga atacacagat atcaaagcga ttatcaaaac agcatgggat tggcatgtca
     1021 gccatccaaa tggttatgaa gaatagggga atgagatatg tcaatcagtc aaacgatttg
     1081 tgattttgcc acacttgcga tcgaagctgg cggatggatg gaaatggacc gcttatattt
     1141 acagaatcgt cttttaggga tggtaggaga agatgctcta gaaagcattg aagtaagacc
     1201 agttaaggaa gaatctgtag acttattgga tcagcttgtc gctaaggcaa gagaaaatgg
     1261 tgttctttca gaattgccag cagaacaaga gatttttgaa gcgcaattaa tggattttct
     1321 cacaccgcca ccttcagtcg tcaatgcatt ctttgcacag cactattcga aaagcccaga
     1381 ggaagcaact gagtatttct ttaatttaag taagaaaaat gactacatca aaacaagagc
     1441 catcgcaaaa aacatcaaat ttccggcaga gtcggagtat ggtgaactgg aaatcacaat
     1501 caatttatct aagccagaaa aagatccaaa acaaatcgca gcagaacgcg aggcggtaaa
     1561 agtagactat cctacctgca tgctttgcat ggaaaacgaa ggatatagag gtcgcttgaa
     1621 ctatccagcc cgcaccaatc accggattat tcgaatgaat ctagacggtg aaagctgggg
     1681 attccaatat tccccttatg cttattacaa tgaacattcg atcattttat cagaacaaca
     1741 ccgcccgatg aaaatcacaa aggaaacatt tgctagactg cttcggatta ctgaagtctt
     1801 gcctcattac tttgttggtt cgaatgctga cttaccaatt gttggaggat cgattctttc
     1861 tcatgatcat tatcaggcag gacgtcatga atttcctatg gcaaaagcac cgatagaaaa
     1921 acttatcaaa ctagaagatt atccgcaagt catcgcagga gtcgtgaaat ggccgatgtc
     1981 tgtgatccgt ttgcaatcaa cagagaaaaa cttgcttatt gaagcagcta actcagtatt
     2041 ggaaaaatgg aaagcatatt cggatgaaac agtatctatc agagcttttt cagcagatgg
     2101 tacaccacac catacgatta cgccaattgc acgaagaagg gacaattact ttgagttgga
     2161 ccttgtcctt cgagacaaca atgtttcaga agaacatcct gatgggattt ttcatccaca
     2221 taaagatgtc caacatatca aaaaggagaa cattgggttg atcgaagtaa tggggcttgc
     2281 tgttttacca cctcgtttga aaccagaact agtagaagta gagcatttct tattaggaga
     2341 agaaaaccag atggctgaat atcatcgtcc gtgggcagaa caattaaaaa aagaacatcc
     2401 tgatttgacg aaagatacgg tcaccgatgt cgtacaaaaa tctgtaggtc aggtttttgc
     2461 acgtgtactg gaagacgctg gagtattcaa gcgagatgaa aaaggacagg cagcgtttga
     2521 acgatttact gcgattttat aaacgtaaaa aaggaggaga gacaaatggc aacaattaaa
     2581 gacattgcgc agttggcagg agtttctcct gctactgttt caagagttct caactatgat
     2641 cctgatcttt ccgttggctt agccacaaag caaaaaattt ttgaagcagc agaacgctta
     2701 aattatacga aacatcgtaa aaatgcagca aatactaagc aaacgcttcg cctgatccag
     2761 tggtacgacg atacggaaga gcttgaagat ctttattatt tatctattcg cctaggaatc
     2821 gaaaaaaagg cagaagaagc aaacgttact ttattgaaag aaacgtggga ttccattagt
     2881 gatcttccgg cagatggaac gatcgcacta ggaaaattcg acgaagctca gttattagaa
     2941 atgaaaggat cccaaaatgc cttgcttttg gtagattctg acggcacttc tcaaggaatc
     3001 gactctctag ttgtcgactt taagtcaagt gttcaaaaag tcattgaaca ctttgtctcc
     3061 cataaagcgc aacatatcgc tatgcttgct ggaacagaat atacgaagaa aaaccatcag
     3121 cggctaaaag atcccagaat tatggcattt caacaagttt tggcagaaaa aggctacgac
     3181 gaaggtccaa ttatcgaagc agatttttct gtcgagtcag gatatcaagc agtgaagcag
     3241 tatttaaaaa cgaatccgca tgttcctgat gcgctttttg cagctaatga tgcattagcg
     3301 attggcgcac tgaaagcgat tcaagaaaaa ggtttagtgg taccagaaga tatttctgtg
     3361 atgggcttca atgatgtcag tgtagcgaaa tatgtaacgc cctcgctttc tactgtaaaa
     3421 gtttataccg aatggatggg agagctagca gtggagacga ttcttcaatt aagtaattcc
     3481 aaagcaccgg tttcaagaaa gattattgtg gaaacagagt tgattattcg agaatcaacg
     3541 aaataaaaga tggaaaagct gtgggaaaat ggagtgatac cgcagctttt t
//
LOCUS       DAZKCZ010000146         3475 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000146 DAZKCZ010000000
VERSION     DAZKCZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.149"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..322
                     /locus_tag="ACG8KN_002439"
     CDS             <1..322
                     /locus_tag="ACG8KN_002439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189441.1"
                     /translation="KKLKTFFILPEIEESNELTASDRSAFLLPVFNTWGKAPQKQPYR
                     RCSKVSFLVLRQIYAKMWKAIFTKFSLILKTKRLFHNQSLMLDADQLFHNLIDGVLSA
                     ASRY"
     gene            complement(523..798)
                     /locus_tag="ACG8KN_002440"
     CDS             complement(523..798)
                     /locus_tag="ACG8KN_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9189442.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            906..1670
                     /locus_tag="ACG8KN_002441"
     CDS             906..1670
                     /locus_tag="ACG8KN_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFP9189443.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1793..2296
                     /locus_tag="ACG8KN_002442"
     CDS             1793..2296
                     /locus_tag="ACG8KN_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9189444.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            2704..3351
                     /locus_tag="ACG8KN_002443"
     CDS             2704..3351
                     /locus_tag="ACG8KN_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFP9189445.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     regulatory      3393..3459
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT         1165 a          622 c          701 g          987 t
ORIGIN      
        1 taaaaaacta aaaacttttt ttattctgcc cgaaatagag gagtcaaatg aactcactgc
       61 aagtgataga tcagcttttt tactacctgt ctttaatact tggggcaaag cgcctcaaaa
      121 acaaccttat cgacggtgtt caaaagtcag tttcttggta ttaagacaaa tttatgcaaa
      181 aatgtggaaa gccattttca caaaattttc cttaatactc aaaactaagc gactttttca
      241 caaccagtcc ttaatgcttg atgcagacca gctttttcac aaccttattg acggtgtttt
      301 atcagctgcc tctcggtatt aagtcattac atttcaaaat atgagaagct attttgaaaa
      361 tgtttctcct taatactcga ggcaaagcgc ttcaaaaaca accttatcga cggtgttcaa
      421 aagtcagttt ctcggtatta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa
      481 aattttcctt aatactcaaa actaagcgac cttttcacaa ccttatccca ccacatcaaa
      541 tttctttcgt tcctcaatca tcggatcttc ttggacatcc acatactgca ctcgtccact
      601 tgggctcgga gacttcttga cttcttcgat aaatttttgg atgatgtcgt catttccgat
      661 tgcttcgatt gtgactgagc cgtcgtcttc gttttttacc gttccggtca cgcctaactg
      721 atcagcaacc atcttggtca tatagcgaaa tcccacgccc tggacacgtc cttggacatt
      781 cattttaact ttgcgcatgt tcgtcacctt tctttcctgt tttataaatc aatgataccg
      841 atttttttag gatgaaacaa gctcaagcac ctattccatg atataatcag gtgaggtgaa
      901 ggaatgtgaa agagatccaa tcagcaaaaa atccaagaat caaagaatta aaaaaactac
      961 ataaaaagaa atacagagaa gaaaaggcag aatatctttt ggaagggttc catctgatcg
     1021 aagaagctgc gtcgtctgat gcagaaataa aagaactttt tgtgacacag agaggtatga
     1081 aagaatgggg agactggctg gctgtaaaca aagagatttc tacttttcta gtttcagatg
     1141 aggtcatgac cgctatgtca gatctaccta caccgcaagg gatcttagca gtgatgacgt
     1201 taacatctga agatacgcca gatttttcag gaggctggct tttactagat aatgtacagg
     1261 atcctggaaa tgtgggaacg atgatccgta cggcagatgc gtttggtatg gctggtgttt
     1321 tattaggtaa aggaacggca gatatttata gtacaaaggt gctgcgaagc atgcagggaa
     1381 gcaattatca tttgcctgtg ttacaaaaag atttggcaga tgtaatccct gtaatgaaag
     1441 gcaaagggtt tacggtgtac ggtacagaat taaatgaaga agcacatgca ttagacaaag
     1501 tagaaaaaac agaggatttc gctataatca tgggaaacga aggtcaggga gtcagtcagg
     1561 aaatcttgtc tttgactgat cagaacgtat atatcccaat gaaaggaaat gcggaatcat
     1621 tgaatgtcgg agtcgcagca gggatcgttc tttatcattt ttctttatga ttaaggaact
     1681 attaagcttt agctatttta tctagagcga ctagaataat ccgaacactg gtggtatact
     1741 tagatcaatt tcagaagaaa atgaaattca aaaaggtggc agatcgataa ttatgacaga
     1801 aaaatggcgt gaagacgaag aatatatgtc ttatgtacaa gatcttttag aaacagaagc
     1861 agtgaaaaaa ttagcgaatt acacacagca tgttcattcc actcgtttgg agcattccat
     1921 cagtgtttct tactatagtt atctgctcgc aaaaaaatgg ggtggcaatg ctaaagcaac
     1981 agcgcgtgct ggacttttgc atgatctgtt ctactatgat tggcgtacga cgaaatttga
     2041 cgaggggaca catgcgtata tccatccaag aattgctgta aagaatgcag aaaaaatcac
     2101 cgatttgtcg gatcttgagc gagatatcat tttgaagcac atgtggggtg caacgatcgc
     2161 accgcctaag tataaagaag gatatattgt gacattcgtg gacaaatact gcgcagtcaa
     2221 agaagcggct cagcccatgt ctgcaagtat gcgtaagaga tggcaacgtt actttgggaa
     2281 agaatcctcg atttaacagg taaaaccaat aatagaccat attgaggttg tgacagaagt
     2341 ggtcagcttc aagaaataag aaggaattta cgaaaattac ttctcaaatt tttgtgaagt
     2401 ttcggcttat tactgcagat gcaagctttt gaacaccatt cattcgaaac aagggacttt
     2461 gacacgactt ttgtcacagt ccttttttct gtttgcagtt gactatctgt tcaagatacg
     2521 gtttactttt atttatcgaa tgaatcatgt acatagaata ataagcatta aagggggtga
     2581 tgataagatg caaaacgtat cttatttaaa agagtccagg atcttcaggt aacacatact
     2641 ttgcagaaga tcactttttt ctacaaagta tgcactttgg ttagaagaaa ggactttaaa
     2701 atcatggaac aaacaataaa accataccaa tattttttta taataagaga agtagaacag
     2761 ttattaaatg cgtactcatc tgtcaatgat ccaaaaacgg tacaaacagt tcaggcacta
     2821 gcagctgaaa agattcgaga catattagat cacgaactgc cagaaatcga tacattttta
     2881 acgacagttt tagacgtaaa actgacaaaa gcgcaagcag aaagagcatt ggaggagctg
     2941 aagcaaatcg ttcagccgtt tatgcagcct agcaaaccac aaatcgagaa attattccga
     3001 aaagtgaaaa aactcaaaca accagcttgg aatgaaatcg acttgcaaga acatacgtac
     3061 atcggctgga atgatccagg tacgcagaaa aaattcatgg tctattacga agatgaaaaa
     3121 ttaaaaggga ttagcggtac tttatcacca acgataaata agggaatctg tgcaatctgt
     3181 cagaaaacat cgaatgtttc gctttttctt tctacaacga aagcaggttc tgatggcacg
     3241 tatacgaaaa aggggaatta tatttgtcat gatagtgacc aatgtaatca acagttaacg
     3301 aaattggctc ctttctatga atttgctgaa cgtgtaaaga aagaacgata attttattgt
     3361 tttttacaga aaaatatgct atcttttaat ggaagtgaat aggtgatggt gttcgccttt
     3421 aaatgctaac gaaagatagc taatgacgcc tactaaagtc tggggacagc ccctt
//
LOCUS       DAZKCZ010000147         3459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000147 DAZKCZ010000000
VERSION     DAZKCZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.150"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="ACG8KN_002444"
     CDS             complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="ACG8KN_002444"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9189446.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="ACG8KN_002445"
     CDS             complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="ACG8KN_002445"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9189447.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="ACG8KN_002446"
     CDS             complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="ACG8KN_002446"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9189448.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2589..3311)
                     /gene="tsaB"
                     /locus_tag="ACG8KN_002447"
     CDS             complement(2589..3311)
                     /gene="tsaB"
                     /locus_tag="ACG8KN_002447"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9189449.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT          988 a          757 c          594 g         1120 t
ORIGIN      
        1 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
       61 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
      121 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
      181 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
      241 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
      301 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
      361 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
      421 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
      481 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
      541 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
      601 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
      661 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
      721 tcagggagtt tagttgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
      781 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
      841 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
      901 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
      961 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1021 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1081 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     1141 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     1201 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     1261 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     1321 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     1381 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     1441 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     1501 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     1561 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     1621 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     1681 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     1741 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     1801 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     1861 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     1921 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     1981 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2041 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     2101 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     2161 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     2221 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     2281 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     2341 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     2401 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     2461 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     2521 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     2581 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     2641 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     2701 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     2761 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     2821 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     2881 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     2941 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3001 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3061 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     3121 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     3181 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     3241 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     3301 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     3361 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     3421 tgctggcggg caaattttgc cccttcctga aacacaaaa
//
LOCUS       DAZKCZ010000148         3311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000148 DAZKCZ010000000
VERSION     DAZKCZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.151"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1825
                     /locus_tag="ACG8KN_002448"
     CDS             <1..1825
                     /locus_tag="ACG8KN_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HFP9189450.1"
                     /translation="IIGNPSLNHRYRKGFYSLFKTLQQNHLVEQGGSRRGAQMIMLND
                     WHPDIFEFIISKIQNPAILQNLIHTSQSPTVRQLASEKLTFEPLTANEKEWLEFLVDH
                     PTNDMEDKFLKTAQQKLKAGGTYSVKDPDFLTGANISVCLTKEFMEAVETDQTYELRF
                     PAIEDYDASEMADYDQHWMEVGDVREWAETGRKVKIHQTIKARELWDLITYCATYSAE
                     PGIFFIDNANEMTNAQAYGQKVVATNPCGEQPLTPYAVCNLAAINLANMVDKKTKVVD
                     FEKLTDVVRASVRFQDNVIDATPYFFKKNEQQAKGERRVGLGVMGLADLLIYCEKVYG
                     SEDGNRLVDQVFEKIAVTAYQTSVELAKEKGSFPFLVGKTAEETTALRQRFIESGYMK
                     KMPESIRQAILKYGIRNSHLLTVAPTGSTGTMAGVSTGLEPYFSFSYFRSGRLGKFIE
                     VNAEIVSEYLEKNPEKTADNLPPFFVSAMELSPKAHVSVQCTIQRWVDSSISKTVNAP
                     KGYQVKQVAAIYEDLYQGGAKGGTVYVDGSRDSQVLTLEADDNVFEEPVVGVADNQVT
                     LDSLESSEAEEICPICHEGRIEEIGGCSTCTNCKVQLKCGL"
     gene            1839..2408
                     /locus_tag="ACG8KN_002449"
     CDS             1839..2408
                     /locus_tag="ACG8KN_002449"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP9189451.1"
                     /translation="MKIYTKSGDKGNTSTSIIGGERIKKSDARVCAYGSVDEVNSWVG
                     KIISELDDKKFELLKKELVVLQILLFDIGTDLATPIGIKEMIVGKEEVTKIETLIDFY
                     QAKVPVIEKFILPGGHPVACDMQITRTVIRRAEREITRLIVSDEPINLHAYKIMNRLS
                     DLFFVLARYVNHVYATKEPFYERAGKVFH"
     gene            2424..2828
                     /locus_tag="ACG8KN_002450"
     CDS             2424..2828
                     /locus_tag="ACG8KN_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFP9189452.1"
                     /translation="MKKLATLLTVFIGLFIMTGCQSNQNDQASSKASTQATSEVAKSS
                     ALIKLTDSDKEITSKTVTYKSGESLLVILKANFEVKERDGFITSIDGHAQDESQNKYW
                     TFTINGKMGEKGAGETTLDNDDQVVFNLGVFK"
     gene            3030..3272
                     /locus_tag="ACG8KN_002451"
                     /pseudo
     CDS             3030..3272
                     /locus_tag="ACG8KN_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         1141 a          505 c          703 g          962 t
ORIGIN      
        1 tattattggg aatcccagct taaatcatag ataccgtaag ggattttatt ctttatttaa
       61 aactttacaa cagaaccatt tagttgaaca aggcgggagt cgtcggggag cacaaatgat
      121 catgttgaat gattggcatc cagatatttt tgaattcatt atctctaaga ttcagaatcc
      181 ggccatttta caaaatttga tccacactag ccaaagtccg acagtgcgcc aattagctag
      241 tgaaaaactg acttttgaac cactgacagc aaatgaaaaa gaatggttag aatttttggt
      301 tgatcatccg acaaatgata tggaggataa atttttaaaa acggctcaac agaaattaaa
      361 agcgggtggt acgtacagtg tgaaagaccc tgatttcttg accggagcca atatctccgt
      421 ctgtttaaca aaagaattta tggaagctgt cgaaaccgac caaacttatg agttacgctt
      481 tccagcaatc gaagactatg acgctagtga aatggctgat tatgatcaac attggatgga
      541 agttggagat gtcagagaat gggctgaaac agggcgtaaa gtaaaaatac atcaaacgat
      601 caaggctcgc gaattgtggg atttgattac ttattgtgca acgtattcag cagaaccggg
      661 aatatttttt atcgataatg ctaatgagat gaccaatgct caagcctacg gacaaaaggt
      721 agttgcaacc aatccatgtg gggaacagcc attgacgcct tatgcggtat gcaatctagc
      781 agcaattaat ttagccaata tggttgataa aaaaacgaaa gttgtcgact ttgaaaaatt
      841 aactgatgtc gtaagagcta gtgttcgttt tcaggacaat gtaattgatg cgacgcctta
      901 cttttttaaa aaaaatgagc agcaagctaa aggtgaacga cgtgttggac ttggggtcat
      961 gggattggca gatttattga tttattgtga aaaagtctat ggatctgaag atggaaaccg
     1021 tttagtagat caagtatttg aaaaaattgc tgttacggct tatcaaacat ctgttgaact
     1081 ggcaaaagaa aaaggcagct ttccattttt agttggtaaa acagctgagg aaacaaccgc
     1141 gcttcgtcaa cgttttattg agtcgggcta tatgaagaaa atgccggaat cgatccgaca
     1201 agcgattcta aaatacggca ttcgtaattc tcatttacta acagtcgcgc caacgggatc
     1261 aacgggaact atggccggtg tttcaactgg actagaacca tatttctctt tttcttattt
     1321 tagaagtggt cgtttaggaa aattcattga agttaacgct gaaatagtca gtgaatattt
     1381 agaaaaaaat ccagagaaaa ctgcggataa tctaccacca ttctttgtct ctgccatgga
     1441 gctttcacca aaagctcacg tgagtgttca atgtacgatc caacgttggg tagatagctc
     1501 tatctcaaaa acagtcaatg cacctaaggg ttatcaagtg aaacaagtag ctgcgatcta
     1561 tgaagatctg tatcaaggcg gtgctaaagg tggaactgtc tatgtggatg gtagcaggga
     1621 ttcgcaagtg ttgacacttg aagcagatga caatgtattt gaagaacctg tagtaggggt
     1681 ggcagataat caagttactc ttgactcatt agaatcatca gaagcagaag aaatatgtcc
     1741 gatttgccat gagggtagaa ttgaagaaat tggtggttgc agtacctgca ctaactgtaa
     1801 agtacaacta aaatgtggct tgtaaaagga gctattcgat gaaaatttat actaaaagcg
     1861 gcgataaagg aaatactagt actagtatta ttggtggaga aagaataaaa aaatctgacg
     1921 ctcgcgtttg tgcttacggt agcgttgatg aagtaaattc ttgggtcggt aaaattattt
     1981 cagagttgga tgataaaaag tttgagctgt tgaaaaaaga actagttgta ttacaaattt
     2041 tactttttga tattggaaca gacttggcta cacccatcgg gattaaagaa atgattgtag
     2101 ggaaagaaga agtaactaag attgaaacgc tgattgattt ttatcaagca aaagttccag
     2161 tcattgaaaa atttatctta cctggaggac atccggtggc atgcgatatg caaattacac
     2221 gaacagttat tcgtcgggca gaacgtgaaa tcacacgatt gattgtttca gatgaaccta
     2281 tcaatttaca tgcatacaaa attatgaatc gcttatcaga tttatttttt gtacttgcac
     2341 gttatgtaaa tcatgtttat gctaccaaag agccatttta tgaacgagca gggaaggtat
     2401 tccattaaga gagggtttta aacttgaaaa aattagcaac attattaacc gtttttatcg
     2461 gactattcat tatgacaggg tgccaatcaa atcaaaatga tcaagcctct tcaaaagcat
     2521 ccactcaagc aacaagcgag gttgctaaat caagcgcgct gataaaattg actgatagtg
     2581 ataaagagat cacttcaaaa acagtcactt acaaatcagg agaatcatta ttggtaattt
     2641 taaaggctaa ctttgaagta aaagaaaggg atggctttat tacttcaatt gatggtcatg
     2701 cgcaagatga gagtcaaaat aaatactgga cattcactat caatggaaaa atgggtgaaa
     2761 aaggcgcagg tgaaactaca ttagataatg atgatcaggt agttttcaat ttgggtgtat
     2821 ttaaataaaa attatcgttc tttacagcaa ttgagcgata attaagtaag agaaaattat
     2881 tagtttttaa aggaatatgt taattttaaa acacgttcaa accaatcatt tcgaaggggt
     2941 tgaacgtgtt ttttggttct gttgcaaagt tttatctaaa tacctattta gtgtaaaata
     3001 gctgaaacta aaacataagg agaattacca tgtccaattt taaaggtaaa caatttgaaa
     3061 aggatattat catggtagcc gtatgctact atttaagata taacttaagt tatcgtgatg
     3121 tttcagaaat ccttaacgaa cgtggaatca atgtttgtca cacgacggtt tatcgatggg
     3181 tacaagaata tagtcaagtt atttattgct tatggaagaa aagaaataaa tctgttggcg
     3241 attcctggag aatggatgaa acttatatta agggttctgt tgcaaagttt taaataaaga
     3301 ataaaatccc t
//
LOCUS       DAZKCZ010000149         3306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000149 DAZKCZ010000000
VERSION     DAZKCZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.152"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..257
                     /locus_tag="ACG8KN_002452"
     CDS             33..257
                     /locus_tag="ACG8KN_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189453.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            272..1141
                     /locus_tag="ACG8KN_002453"
     CDS             272..1141
                     /locus_tag="ACG8KN_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP9189454.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1122..1856
                     /locus_tag="ACG8KN_002454"
     CDS             1122..1856
                     /locus_tag="ACG8KN_002454"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9189455.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1889..2797
                     /locus_tag="ACG8KN_002455"
     CDS             1889..2797
                     /locus_tag="ACG8KN_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9189456.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2794..3274
                     /locus_tag="ACG8KN_002456"
                     /pseudo
     CDS             2794..3274
                     /locus_tag="ACG8KN_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1153 a          469 c          729 g          955 t
ORIGIN      
        1 taatattctt agcgtaaact tggaggtgct gtatgactac ggcagaaatg attaaagaac
       61 tgtgtgagca aatgaatata agtgtttccg aacttgctag acgtattggc cagactccac
      121 agaatttcaa taaaaaatta caacgagaaa cagtaacctt ggatgagttg aaggccattg
      181 ctgatgtgct aggtgtcaag tttgtgcagg catttatttt accagatgga gaagaaataa
      241 aaatatctaa cgaataaagg aggatgttta tatggtaggt aatgttattc aaatagtaac
      301 agagaaatta tcatctttgc cttttataga aggcatcgta ttagggggct cacgtgcaag
      361 aggcacccat acagaaaatt ctgatataga tatcggaatc tattacaatt cagactcatt
      421 tgacctgaca gcgattaatc aaattgctac agaattggat gataaaaata gaaacaacct
      481 tgttgtgcct cccggagcgt ggggtgattg gattaatggc ggcggatggt tatttataaa
      541 tgggtatcat gttgacttga ttttacgtga tataaaacgg gtagaacaaa taatcaaaga
      601 tacggagcaa ggaattgtta ctgccaatta tcagaccggg catccccatg gttatataag
      661 tgcaatgtat cgtggagaat tagcgattag taaaatacta tatgctaaga acgaaagctt
      721 atgcgaatta aaaaagcagg cagaaattta ccctactgct ctaaagaaaa gcttgatgaa
      781 cttttttata tttgaagctg aattctcttt aatgtttgta aaagcaaatg cgggggtaga
      841 ggataaatat tatattgcag gccatgtttt tcgtattatt tcatgcttaa atcaagtact
      901 atttgcatgt aataatgctt attgcattaa cgaaaagaaa gctataaaac tgcttgaaac
      961 ttttgaatat aaacctgaaa aatatgccga aagagttaat catatttttg aagtactcgg
     1021 tctttctctt tttgaatgct acgatatgac cgagaaactt tataaagaag tgaatgaaat
     1081 tgtatcggag ataaataact ttttaaacga ggagagttca gatgaaagaa aacaaatatg
     1141 atgataatat attttttcaa aaatacagtc aaatgagtcg ctcgcagaaa ggactggctg
     1201 gtgcgggaga atgggagact ttgaaaaaga tgctacctga ttttaagggt aagcgtgtgc
     1261 ttgatttagg atgcggctat ggatggcact gtatatatgc gatggaaaac ggtgcttcct
     1321 ctgtagtagg tgttgatatt tctcataaaa tgctcgaagt agcaaaagga aaaacccatt
     1381 ttccacagat tgaatatgaa tgctgtgcca tagaagatgt ggatttccca gaggagagct
     1441 ttgatgtaat actaagttcg cttgcgtttc attatgtagc agactatgag aatttaataa
     1501 aaaagatata taggatgctg aaggctggtg gcaatttagt ttttacagtt gaacatcctg
     1561 tttttactgc tcatggaaca caagactggt attataacga aaaaggagaa atactgcatt
     1621 tcccggtgga caattattat tatgagggca aacggacagc tatgtttttg gaagaaaagg
     1681 ttacaaaata tcatagaaca ctgaccacat atctaaatac actgctttca aatagtttta
     1741 taataaatca gattgtggag ccacagccgc cagagaacat gatggatatt ccggggatgg
     1801 cggatgaaat gcgacgccca atgatgctga ttgtatcggc aaaaaagaag atgtaataat
     1861 atagaaaaaa taaacgagga gtatgtaaat gagatcagaa aaagaaatga tggatttagt
     1921 actttcttta gcagaacagg atgaacgtat tcgaattgtg acccttgagg ggtcacgcgc
     1981 aaatattaat atacctaaag atgaatttca ggattatgat attacatatt ttgtaagtga
     2041 tatagaaccg tttatatcta atgatgactg gcttaatcaa tttgggaata taataatgat
     2101 gcaaaagccg gaggatatgg aattattccc acctgaagaa aagggatttt cctatcttat
     2161 gctatttgat gattacaata aaattgatct taccttattg cccttggaag agttagataa
     2221 ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag
     2281 ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga
     2341 tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa
     2401 ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat
     2461 gatatcatgg aaggtcggca tcgaaacagg ctttaaatta agtgtaggca agaactataa
     2521 gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat
     2581 ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt
     2641 atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac
     2701 aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac
     2761 atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac
     2821 ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg
     2881 tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc
     2941 taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat
     3001 actgataaga taatatatct tttatataga agatatcgcc gtatgtaagg atttcagggg
     3061 gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt
     3121 gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat tctatcataa
     3181 ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     3241 tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt
     3301 cgtctt
//
LOCUS       DAZKCZ010000150         3304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000150 DAZKCZ010000000
VERSION     DAZKCZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.153"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            238..1629
                     /locus_tag="ACG8KN_002457"
     CDS             238..1629
                     /locus_tag="ACG8KN_002457"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9189457.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            1667..2497
                     /locus_tag="ACG8KN_002458"
     CDS             1667..2497
                     /locus_tag="ACG8KN_002458"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9189458.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2469..>3304
                     /locus_tag="ACG8KN_002459"
     CDS             2469..>3304
                     /locus_tag="ACG8KN_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9189459.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQ"
BASE COUNT         1160 a          463 c          640 g         1041 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaagtattc cttactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc ctcacaagga
     1561 ctagatgaag atgaaaatat cagttcatta gcagaaagtg catttccaaa agaaaaaacg
     1621 tatcgttaga ttggaaaaat tttgagcaaa gaagtaggag agaacattgg aaaataaaaa
     1681 tataagtata ctgctttctt tgtatgataa agaaaagccg gaatatttaa aggaagcatt
     1741 agctagtatc tttgagcaaa cggtgatgcc ggcagaaatc gtattggttt atgatggccc
     1801 tataaatgag aagcttcagg cagttgtcac ttatttccag caaaaagttc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ctggattaga
     1921 acatactaaa aatcgcttag tggccagaat ggatacagat gacatcatgg aaccacacag
     1981 attggaaaaa caattagaga tcttccaaga atatccagaa atagcgattg ttgggagtaa
     2041 tattgaagaa tttactggca cacttagcaa tgttatcgga agaaggattg tacctgagta
     2101 taatgatgaa atccgtgtgt tttcgaaaag aagaaaccct tttaatcata tgacagtcat
     2161 gtttgataaa aatgctattt tagaagtggg aaactatcgc ccattaattg gatttgaaga
     2221 ttattatttg tgggtaagac tattgaaggc gggatataaa ggatataata tccaagagat
     2281 tctagtacat gctagagcag gtgctgacat gtatgctaga cgtggaggaa gtaaatatct
     2341 tttaccagga attaaaggcc gatatcgcgt atggaaagat ggtctaggat cactaaaaga
     2401 ttttctgata gttatttgcg gacatcttgt aattagttta ttaccgaata aaattagggg
     2461 aaaagtatat gaatcaaaac ttagaaaaac aaactaaact ttctataatc ataccttttt
     2521 acaatgcaaa gaatacttta agaagagctg tagacagcgt cttaacgcaa ggttatccca
     2581 atattgaaat cttgttaatt gatgatggat ctacagatgg tagtagtgac attggtgacg
     2641 aaattgctaa tctagagagt aaagtagcat gttatcactt ggaaaacgct ggtccatcag
     2701 ttgctaggaa ctatgggata aaagaagcaa ctggaaattt ggtaggattt ttagatagtg
     2761 acgattattt tttacctgat tgttttaata atgttttgaa agactaccat aggaatcacc
     2821 ccgatatcat tgcttttgga ttaagtaaag gaaaaaatat agaaaatgca aatttatttg
     2881 ttcccgaaaa gaagattggt tgttcaagtg aagaagcaat tgagcaaatg tttagaagta
     2941 aggcagtgga cttttacgta tggaataaat tttttaaaag ggaattattc aatcatatag
     3001 tttttccaga aaataaatta tatgaggata tggttcctat gtatgaagct tttagggcgg
     3061 ctagtaaaat tgatattttg ccattttcag gtatttttta ttatcaaaat aacgatagca
     3121 tcgtttatca gtcttttaat tcaaaacagt atgataatat tttgcagaga aaaatattag
     3181 ttgatgaagt aaagaaagat tatcctcgac ttattaattt ggccaatgct cggttagtag
     3241 acggatatct ttcaacaggg tttaaaatta ctggcaaaaa tcaaaatcag aaagaaaaac
     3301 aaaa
//
LOCUS       DAZKCZ010000151         3286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000151 DAZKCZ010000000
VERSION     DAZKCZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.154"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8KN_002460"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8KN_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFP9189460.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8KN_002461"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8KN_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          909 a          731 c          529 g         1117 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatag
//
LOCUS       DAZKCZ010000152         3197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000152 DAZKCZ010000000
VERSION     DAZKCZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.155"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(338..700)
                     /locus_tag="ACG8KN_002462"
     CDS             complement(338..700)
                     /locus_tag="ACG8KN_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9189461.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            923..1675
                     /locus_tag="ACG8KN_002463"
     CDS             923..1675
                     /locus_tag="ACG8KN_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9189462.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1815..2738)
                     /locus_tag="ACG8KN_002464"
     CDS             complement(1815..2738)
                     /locus_tag="ACG8KN_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9189463.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2753..3025)
                     /locus_tag="ACG8KN_002465"
     CDS             complement(2753..3025)
                     /locus_tag="ACG8KN_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189464.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1048 a          603 c          521 g         1025 t
ORIGIN      
        1 tgaacaaata taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat
       61 aatacgtctt tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat
      121 tgaatttttt tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg
      181 aaaaaatatc acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg
      241 aaacaaaaat ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata
      301 tggagcaggt taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt
      361 tttgttctat cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta
      421 gctcctgttt cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat
      481 gaatcgcata gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt
      541 gctttttatc atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt
      601 aataattaat agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa
      661 tatttaaatt actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat
      721 agtcgattta gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt
      781 tacaattgaa cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa
      841 tttcgtgaga aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt
      901 acatttgtca ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg
      961 agtcagtccg agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat
     1021 caattcttca ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt
     1081 attttatcga acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc
     1141 ttttgaccac cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca
     1201 aatcgatacc ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa
     1261 aatgagtgac aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga
     1321 atcctatgga caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga
     1381 aaaatggatg aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat
     1441 gttatttgat ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat
     1501 cttccaagct cataagaagt ggttctctta cagctggcct gaatatcagc caaaagcgca
     1561 taaaggacta ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg
     1621 aagtggtcat ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt
     1681 tacatgatcc aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca
     1741 agttagattt ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc
     1801 tactttaaat atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt
     1861 tctgtaagaa acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt
     1921 gggaaaccaa gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta
     1981 tcttgcagat agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca
     2041 tcaactccaa gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa
     2101 tcccaaaaaa caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg
     2161 atttttgagt tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata
     2221 ccttgctgct tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca
     2281 cagcttgcct gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat
     2341 gccaataaat catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta
     2401 gctgcaaatt cttcaagata ccttcgctct ttttctttat gatagcccgt cggtaaaagt
     2461 gtcactttgt aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa
     2521 tacggatgat tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac
     2581 tgtctggcat ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc
     2641 cttttttcct catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa
     2701 taacctgtga gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa
     2761 aaagattttc ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt
     2821 ttccttcatg cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt
     2881 cgatttcatt cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca
     2941 acgttaatac ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat
     3001 ctgttatctc tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt
     3061 atgaaacgcg tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc
     3121 attattacca aagtctctta ttttaaattg aatacattat aatgatactg taaatatatc
     3181 tttattatta gtgaatg
//
LOCUS       DAZKCZ010000153         3193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000153 DAZKCZ010000000
VERSION     DAZKCZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.156"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(554..2167)
                     /locus_tag="ACG8KN_002466"
     CDS             complement(554..2167)
                     /locus_tag="ACG8KN_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9189465.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..>3193)
                     /locus_tag="ACG8KN_002467"
     CDS             complement(2308..>3193)
                     /locus_tag="ACG8KN_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9189466.1"
                     /translation="QSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANLYAEFAHKS
                     IELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEITSI
                     ITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLSSL
                     PRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQIFF
                     ISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSIHHFSS
                     AFKKYTGLSPSKYYLSLK"
BASE COUNT         1029 a          548 c          569 g         1047 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tgg
//
LOCUS       DAZKCZ010000154         3176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000154 DAZKCZ010000000
VERSION     DAZKCZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.158"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..269
                     /locus_tag="ACG8KN_002468"
     CDS             <1..269
                     /locus_tag="ACG8KN_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9189467.1"
                     /translation="GLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDTGGAG
                     LGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            484..1452
                     /locus_tag="ACG8KN_002469"
     CDS             484..1452
                     /locus_tag="ACG8KN_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9189468.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1445..2476
                     /gene="vanA"
                     /locus_tag="ACG8KN_002470"
     CDS             1445..2476
                     /gene="vanA"
                     /locus_tag="ACG8KN_002470"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9189469.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2482..3090
                     /locus_tag="ACG8KN_002471"
     CDS             2482..3090
                     /locus_tag="ACG8KN_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP9189470.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          938 a          606 c          781 g          851 t
ORIGIN      
        1 cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa cactggaagc atcccaaaag
       61 ataagctagc tgccatattt gaaaagttct ataggctgga caatgctcgt tcttccgata
      121 cgggtggcgc gggacttgga ttggcgattg caaaagaaat tattgttcag catggagggc
      181 agatttacgc ggaaagcaat gataactata cgacgtttag ggtagagctt ccagcgatgc
      241 cagacttggt tgataaaagg aggtcctaag agatgtatat aattttttag gaaaatctca
      301 aggttatctt tactttttct taggaaatta acaatttaat attaagaaac ggctcgttct
      361 tacacggtag acttaatacc gtaagaacga gccgttttcg ttcttcagag aaagatttga
      421 caagattacc attggcatcc ccgttttatt tggtgccttt cacagaaagg gttggtctta
      481 attatgaata acatcggcat tactgtttat ggatgtgagc aggatgaggc agatgcattc
      541 catgctcttt cgcctcgctt tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa
      601 tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg tgggacataa atcagagatt
      661 tccgcctcta ttcttcttgc gctgaagaga gccggtgtga aatatatttc tacccgaagc
      721 atcggctgca atcatataga tacaactgct gctaagagaa tgggcatcac tgtcgacaat
      781 gtggcgtact cgccggatag cgttgccgat tatactatga tgctaattct tatggcagta
      841 cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac
      901 cgtggcaagg tactcagcga catgacagtt ggtgtggtgg gaacgggcca gataggcaaa
      961 gcggttattg agcggctgcg aggatttgga tgtaaagtgt tggcttatag tcgcagccga
     1021 agtatagagg taaactatgt accgtttgat gagttgctgc aaaatagcga tatcgttacg
     1081 cttcatgtgc cgctcaatac ggatacgcac tatattatca gccacgaaca aatacagaga
     1141 atgaagcaag gagcatttct tatcaatact gggcgcggtc cacttgtaga tacctatgag
     1201 ttggttaaag cattagaaaa cgggaaactg ggcggtgccg cattggatgt attggaagga
     1261 gaggaagagt ttttctactc tgattgcacc caaaaaccaa ttgataatca atttttactt
     1321 aaacttcaaa gaatgcctaa cgtgataatc acaccgcata cggcctatta taccgagcaa
     1381 gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt tggattttga aaggagacag
     1441 gagcatgaat agaataaaag ttgcaatact gtttgggggt tgctcagagg agcatgacgt
     1501 atcggtaaaa tctgcaatag agatagccgc taacattaat aaagaaaaat acgagccgtt
     1561 atacattgga attacgaaat ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg
     1621 ggaaaacgac aattgctatt cagctgtact ctcgccggat aaaaaaatgc acggattact
     1681 tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat gtagcatttt cagctttgca
     1741 tggcaagtca ggtgaagatg gatccataca aggtctgttt gaattgtccg gtatcccttt
     1801 tgtaggctgc gatattcaaa gctcagcaat ttgtatggac aaatcgttga catacatcgt
     1861 tgcgaaaaat gctgggatag ctactcccgc cttttgggtt attaataaag atgataggcc
     1921 ggtggcagct acgtttacct atcctgtttt tgttaagccg gcgcgttcag gctcatcctt
     1981 cggtgtgaaa aaagtcaata gcgcggacga attggactac gcaattgaat cggcaagaca
     2041 atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt
     2101 attgggaaac agtgccgcgt tagttgttgg cgaggtggac caaatcaggc tgcagtacgg
     2161 aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac
     2221 cgttcccgca gacctttcag cagaggagcg aggacggata caggaaacgg caaaaaaaat
     2281 atataaagcg ctcggctgta gaggtctagc ccgtgtggat atgtttttac aagataacgg
     2341 ccgcattgta ctgaacgaag tcaatactct gcccggtttc acgtcataca gtcgttatcc
     2401 ccgtatgatg gccgctgcag gtattgcact tcccgaactg attgaccgct tgatcgtatt
     2461 agcgttaaag gggtgataag catggaaata ggatttactt ttttagatga aatagtacac
     2521 ggtgttcgtt gggacgctaa atatgccact tgggataatt tcaccggaaa accggttgac
     2581 ggttatgaag taaatcgcat tgtagggaca tacgagttgg ctgaatcgct tttgaaggca
     2641 aaagaactgg ctgctaccca agggtacgga ttgcttctat gggacggtta ccgtcctaag
     2701 cgtgctgtaa actgttttat gcaatgggct gcacagccgg aaaataacct gacaaaggaa
     2761 agttattatc ccaatattga ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa
     2821 tcaagccata gccgcggcag tgccattgat cttacgcttt atcgattaga cacgggtgag
     2881 cttgtaccaa tggggagccg atttgatttt atggatgaac gctctcatca tgcggcaaat
     2941 ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt
     3001 gggtttgaag catatagcct cgaatggtgg cactatgtat taagagacga accatacccc
     3061 aatagctatt ttgatttccc cgttaaataa acttttaacc gttgcacgga caaacggttc
     3121 tgttgcaaag ttttaaataa agaataaaat cccttacggt atctatgatt taagct
//
LOCUS       DAZKCZ010000155         3044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000155 DAZKCZ010000000
VERSION     DAZKCZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.159"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..803
                     /gene="fabI"
                     /locus_tag="ACG8KN_002472"
     CDS             48..803
                     /gene="fabI"
                     /locus_tag="ACG8KN_002472"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9189471.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(913..1965)
                     /locus_tag="ACG8KN_002473"
     CDS             complement(913..1965)
                     /locus_tag="ACG8KN_002473"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFP9189472.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2091..3011
                     /locus_tag="ACG8KN_002474"
     CDS             2091..3011
                     /locus_tag="ACG8KN_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFP9189473.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          882 a          616 c          580 g          966 t
ORIGIN      
        1 tttttttacg actatcaaat ttttatcaga aacaaaggag agtcattatg tcatttctag
       61 aaggaaaaaa aatcgtcatt atgggcgtcg ctaacaaaaa aagcattgct tggggttgtg
      121 ctgaagcaat gttgaaacaa ggggcagaaa ttatctatac ttaccaaaat gaacgaatga
      181 aaaaatcatt agtcaaattg gtcggcgaag aagcattcac tgtagaatgt gatgtttcta
      241 gcgacgaaag catcgaaaaa gcaatgaatg ccatcggtga atatgctggg gagatccacg
      301 gccttgtcca tgcagttgct tacgccaaca aagaagaatt gtccggaaat gtgtctgata
      361 tttccagaga cggctatcaa ttagctcaag atatcagcag ctactcttta attgctgtat
      421 caaaatacgc acaaccgttt cttgcaccaa acagcgggat cgttagcatg acttatctag
      481 gttcagaacg cgcaatcccg aattataaca tgatgggtat tgcgaaagct tctcttgaag
      541 ctgctatccg ttatttggca gctgagttct cgcctaaagg aattcgggtc aatggaatct
      601 cagctggggc aatcaaaaca ttagctgtca caggtgttaa agactatcaa aaactgatcc
      661 agttatctga atctcgtacg cctgatcagc aaggtgtgac gatcgaagaa gtcggtaatg
      721 cttgtgcttt cttaatcagc ccactttcca gcggaatcat cggtgatatt atctacgttg
      781 acaaaggtgt ccatctttca taaacttttg ttgcattctt taaagtaaaa catataaaaa
      841 tcaggaaaaa actttttaaa gtctcttcct gattttttct ttttacgttt ttttcaactc
      901 attactttga aattattcca cgacttcaat ccagccttct ggtgcttcta tgtctccaaa
      961 ttgaatacct gtcaattcat catacaaccg ttttgttact ggtcctactt ccgtttcact
     1021 ataaaataca tgaagattgt tattgtgata aatcccaccg attggagaaa taacagccgc
     1081 tgtaccacat gcaccagctt ctgcaaattg atctagatca tcaatgaaaa tatctccttc
     1141 aattgcttgc agtcctagtc gttcttcagc taactgcaac aaggaatatt tcgtgatact
     1201 tggtaagata gagggcgatt gcggagtgat gaattttccg tcttttgtaa ttccaaagaa
     1261 atttgctgca cctacttctt cgattttcgt atgtgtggct ggatcaagat aaacacaatc
     1321 tgagtaatcc agttcttttg cttctgttcc tggaagtaaa ctcgctgcgt aatttccgcc
     1381 gacttttgct gcaccagtcc ccttaggagc agcacgatcg taatcagata caataaaatt
     1441 cgttggtttt agtccacctt tgaaatacgc tcctactggc gtacagaaga cgacaaataa
     1501 atattctgga gctggatgga caccaatgtt ttcaccgacc ccaatcagta aagggcgcaa
     1561 gtataatgta gcgcctgttc catagggcgg cacaaaatct tcatttgctt ttaccacttg
     1621 cttgacagca tcaacgaaca tcttttcagg gaccattggc atcagcaatc gtctagcact
     1681 ttgattcatc cgtcgactat tttgttcagg tcgaaagaga ttgaccttgc cgtcttttcg
     1741 acgataagct tttagacctt caaaacattg ctgaccataa tgaagcgctg gagagccttc
     1801 atgaatcgtc aggtaattat cttctgtcaa ttctccttct tcccattgtc cgtctttcca
     1861 tttggcaata aaacgccaag gagttttgat ataagaaaaa cctagattgt tccaatcgat
     1921 ttgcatatac ttaccacctt cgttattttc tccacatttt atcattatcc atattatttg
     1981 tcattttatt ttcagctttt ttccttcatt atagttattt ttgctatttc cttttttgaa
     2041 aacttcttgg tacaatacag aaagaatcaa attttagaaa agaggatttt atgttttcat
     2101 tattagcaac tgccggtacg acagactctt ctggcgagac attgacagat caagctgtca
     2161 gcaaagtaaa tgcgtttcag cggttttggg acgggattga ttgggacgcc attttggctg
     2221 cctttatcca aaaaagttta tatctgattt ttttgatcgt ccttttttgg attcttcacc
     2281 gtgtgggaaa atatctgatt gataagtctt ttaatcagta ttctaaaaaa gccattctaa
     2341 acgagagccg cttaaagact ttacatagtc ttttaaatac tatttttcat tatacccttg
     2401 gtttcttttt tatctatgca attttatctg ctatcggtgt gccgatcgga tcacttttgg
     2461 ctggtgccgg aattgcaggt gtggcaatcg gtcttggtgc ccaagggttc atgagtgatg
     2521 ttattaccgg attttttatt atcatggaac aacagatgga tgttggggac tacatcaagc
     2581 tagcaaactt aacgattgaa ggaaccgtcg tatctgttgg tatccgtacc cttcagctga
     2641 aagcaacgga tggtactgtc catttcatcc cgaatcgaaa catcaccacg atcagcaatc
     2701 tctctcgtgc aaatatgcaa gtacttttag atattcggat tgttcctgaa gaaggatatg
     2761 acaaaatcta tgagatcatt gatcgagtaa atcgtacatt agctgaaaaa taccaagaag
     2821 agctccaaac tgaaccttct atctttggat tagtggatct tggaaacagc aattttgcga
     2881 ttcgtacggt ctgctacgca ttgaacggaa aacaatatgc cttaaaagaa gagttcttga
     2941 gctcctatgt caaagaattg acagcttctg gtttcacgat tccgaacagt ccaattgctg
     3001 tcggaaaata atttggacaa acgatgcaac gaatctcact aaaa
//
LOCUS       DAZKCZ010000156         3035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000156 DAZKCZ010000000
VERSION     DAZKCZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.160"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1940
                     /locus_tag="ACG8KN_002475"
     CDS             <1..1940
                     /locus_tag="ACG8KN_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP9189474.1"
                     /translation="ISEEEFDNEICASISLNSESQTILEELISNADKGVGSISLFIRP
                     NKAIRKQLFDISTELDSHQFNEFRNSIRLSDYEFYYTSKSNLNFCINENYDKLVGRLN
                     DNIASNPEEDDEALLGSRIDMSDIFKHISVVYVDALRDVLREMKNNRNPVKRIMETIE
                     SKISSDNVESLKTIIQQLNETITSVPEIRKIGENINRQLNSIIGSVYSPNLLLSSTMS
                     DDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKIVEFEACRSRELLNIMLIEEPE
                     AHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVSEISRMNVLKSFNGKSIVMNPA
                     NNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSKGVILVEGDAEEILIPQIVKKL
                     FGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAIVTDLDEQAIPSEHRLYKSTA
                     QEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDVNKENILTYVSPIIDDAFKDKK
                     TITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIELGEKIDFLIKIPKYILNAIAFA
                     CQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTADLNIAIISLLEYFGKDED
                     VVYLINQIENYTKILYAEVSK"
     gene            1937..>3035
                     /locus_tag="ACG8KN_002476"
     CDS             1937..>3035
                     /locus_tag="ACG8KN_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9189475.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCN"
BASE COUNT         1151 a          413 c          508 g          963 t
ORIGIN      
        1 gaatcagcga agaagaattt gacaatgaga tttgtgcttc catttcttta aattctgaaa
       61 gtcaaacaat cttagaagaa ttgatttcaa atgcagataa aggtgtaggt agtatttctc
      121 tatttattag acctaacaaa gctattagaa agcaattatt tgatatttcc actgaattag
      181 actctcatca atttaacgaa tttagaaatt ctattcgtct atccgactat gaattttact
      241 acacatctaa atcaaatcta aatttctgta tcaatgaaaa ttatgataaa ttggtaggaa
      301 gactcaatga caatatagct agtaatcctg aagaagacga tgaagctctg ttgggctcaa
      361 gaattgacat gtcagatatt ttcaagcata tttcggttgt ttatgttgac gctcttcgag
      421 atgttcttag agaaatgaaa aacaatcgaa acccagtcaa aagaatcatg gaaacaattg
      481 aatcaaaaat atcatcagat aatgttgagt cattaaaaac aattatacag caattaaatg
      541 agactattac cagtgttcct gaaattagga aaattggcga aaatattaat cggcaactaa
      601 atagcataat tggttcagtg tattctccta atcttcttct ttcctcaact atgtcagatg
      661 atatgggttc tttagcaaaa tttctttcaa tgaagccaga acaaaatatc gacttagact
      721 tactagggtt aggacatcta aatatgattt atttagcttt gaaaatagtc gaatttgaag
      781 cgtgtcgttc aagagaacta cttaatatta tgttgattga agaaccagaa gctcatattc
      841 atcatcatat tcaaaaaact ctgttcgaag gattaaatct tcaaaaaaac tatactcaga
      901 ttctcatgac cacacattct gtacatttag ctgaagtgtc tgaaatatct cggatgaatg
      961 tattaaaatc gtttaatggc aaatctatcg ttatgaatcc agcaaataat ctgaactcat
     1021 ttgctaaaga aaagttaaaa aagaacaatc taaatctaat aaacgcagta gagagatatt
     1081 tagatgttaa aagaaatgga ttgctttttt ctaaaggtgt tatcttagtt gaaggagatg
     1141 ctgaagaaat acttattcct caaatagtaa aaaaattatt tggtataaac ttagacgaac
     1201 ttggaatagg tttgattaat gttggtagta cttcgtttga atatatcgct agcttgtttg
     1261 acgatgatag aatacagaga aagtgtgcta ttgtaactga tttagatgaa caggcaatcc
     1321 catcagaaca tcgattgtat aaaagcactg cacaagagaa aggtcaagca agaaaagaaa
     1381 aattatcctc cctttttaat gaaaacaatt gggttaattg tttttatgct aatacaacat
     1441 ttgaaattga gtttttagat gtgaataaag aaaatattct aacttatgtc agtccaatta
     1501 tagatgacgc attcaaagat aaaaaaacaa taactgctta taaagaagaa atacaaagtg
     1561 aacaatattt agaaagaaat ttagcgatgt taaaactagc caatcatgtt ggtaaggggt
     1621 ggtttgcaat tgagttaggt gaaaaaattg actttttaat caaaattcct aagtatattc
     1681 ttaatgctat tgcttttgca tgtcaggaaa tagtagatct ctcaatttat aaaaaaattc
     1741 tactccatac aatcgacttg tacgaggaaa ctacagaatt gtcagcttta tcagctagtt
     1801 taaaaacaag tgatacagct gatttgaata tagcaataat ttctttatta gagtactttg
     1861 gtaaagatga ggatgttgtg tatctaatca atcagataga aaattacaca aaaatacttt
     1921 atgctgaggt ttcaaaatga caaatatctt cgaaaaaatc gtaaatgatt taaatttaga
     1981 acaaaaagat gttgtggaaa gtgaagataa cctttatgta aatgcatgtc ctgggagtgg
     2041 gaaaacaagg gtacttacaa ggaaaattgc ttaccaagca attaaacacg atggatcact
     2101 aaaaagaata attgctatca catacactaa tagagctgct gaagaaatta aggaacgcct
     2161 aagtttactt agtatagatg atgatattaa tatttgggtg gggacaatac atcagttttg
     2221 tcttgaattt attatttatc cttttaaaat gaatctctca agagtatcaa aaggatttac
     2281 aattattgat gattatactc aaagagctta cgtcagccaa attaataata tgctgaattt
     2341 aaatctcaaa acgtatgaac tgaacaaaat tgatttgtca ctttctcatg attttaaaat
     2401 taatgaagtt cagtatactc aagtttctga aatgtatcac actatgttgg aaaaaaataa
     2461 agaaattgat tttgatatga ttttactagt agcgtataaa attttaactg taaacagtaa
     2521 cgttgcacgt aatattgcta gtaatataag ggcgatttat gtggatgaat ttcaagatac
     2581 tagagaatta caatacaatg taatagctaa attacttcaa aacaaccctc aaattcagtc
     2641 aatgtttgtt ggagatattg accaagcaat ttatggatca ttagatggta tagctaaatc
     2701 agttgaagaa ctagaaatgc taactagtca cacttttcaa tcaaagacgc tacatggctg
     2761 ttacaggtct aatcagagat tagtagactt ctactctaat tttcagagtt gtccttacga
     2821 gattgaaagc agagggaata ataaaaacga ccgtggtttt ataagttatg attgcaaagt
     2881 tactaaggag gatttaccca acataataaa aaatataata aaggaaaaga ttgaaagcgg
     2941 cattcatgaa aaagatattt gtgtacttgc tcctcaacac tatcctctgt tttcactggg
     3001 tgagtattta aagaaagaat taccgtattg caatt
//
LOCUS       DAZKCZ010000157         3022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000157 DAZKCZ010000000
VERSION     DAZKCZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.161"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..359
                     /locus_tag="ACG8KN_002477"
     CDS             <1..359
                     /locus_tag="ACG8KN_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9189476.1"
                     /translation="ISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLK
                     TISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFY
                     NFRARIYLQQGLIFEN"
     gene            complement(742..1470)
                     /locus_tag="ACG8KN_002478"
     CDS             complement(742..1470)
                     /locus_tag="ACG8KN_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189477.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1448..>3022)
                     /locus_tag="ACG8KN_002479"
     CDS             complement(1448..>3022)
                     /locus_tag="ACG8KN_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9189478.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
BASE COUNT          940 a          489 c          536 g         1057 t
ORIGIN      
        1 caattagtca aacagaaatc gttgatgagc tgttaagcta caacgaagaa ttgcgaactg
       61 cgtatcattt ttgtcaatta ttacgctatt atttcgttaa acgaggtacg gaaggctttc
      121 aagaaaccct taaaacaatt tcatctacgt tacctcagtc gtttaaaaag aaattcacga
      181 tcttccattg ctaccaatcg ggtatttcaa atgcctttaa ggtgtcccac tcaaacggcg
      241 tcactgaggg gctgaataac aagatcaagc tgattaaacg aattgcgttt ggttatcgca
      301 acttttataa ctttcgggca cggatctatt tacaacaagg tctgatcttt gaaaactaaa
      361 aaaatatgcg gcgcacagaa cgcgccgcat atcggactaa gttttattca aatttgtctt
      421 caccaacacg atttgacgaa gagcctattt aaataactac atccatctat tctatcttct
      481 aaaatagcct ttttcatttc agttaaatag ttcagcgcca tttttgcact cgacttgcat
      541 agtagttttt tccatattcc tcaacctcag cgggggttga agacataagg aaaatcctat
      601 gcgttatgct caactggatc tgattaaata ttctaatgta tttttctcta ccatcattaa
      661 atggttaata gccaaaaatc accacttttc aatggctcct tgttgtataa gtataactag
      721 aagatgaaca cgtctgagaa attatagaag ctttttacgg taattactgg ggctgtgtcc
      781 agtagttttt ttgaagagaa cagaaaaata gctcacgtga tctattccta ctttttgact
      841 aatttcatca atagataaga atgtgtcttt cagtagagca cttgctcttt tgattcgaaa
      901 gtctagcaag taatcatgaa aggaataacc tgtttctttt ttgaaggccc gtcctaaata
      961 agcgggatgg taatggaatt gtgtagctac atctttgagc gtaagctttt cactataatt
     1021 gttttgaacg taatcaatca cttgttgaat tgactcatta aaagaatcat cagttagaga
     1081 gttttgatta gctgctgatt tacgcatttt ttcttcttgt actaacttaa ctaaataagg
     1141 aaaatcaatg ggtttcgtta tgtaactttt aatagataaa tcaatcccct tttttgcata
     1201 ctgaaaattt tggtgggcac taatgacaat actagtaaag tagcgattgg atgtttgttg
     1261 caaagattcg attaattcga gtccagaaat tttccccatc gtaatatctg tgataagtaa
     1321 atcaaagcgc tggttttgca ggtttttaaa tgcttcgtct ccgtctgtgt agcagtgatc
     1381 gataatgatg tcggggactt tcttcaaaag ataatgcctt atgccttcta caattgtaca
     1441 atcatcgtca acaattgcaa ttctcaacat gcatattccc ccctttttgt attttgatag
     1501 taatcgttgt tccgacgtgt agttggcttt ctataacaaa acgtgtatct ccaggataga
     1561 gatgctttat acgtcgaaaa gaatttgaga tgccgatatt ctcactataa ggaatatcat
     1621 cattaagttt ttgttgaata tgctccagct tgtcttggtc cattcctttc ccattttcaa
     1681 cgatttttaa cacataatgg ttggttgttt cagttgcata gacagtcaaa cgattttcct
     1741 tttttgatga atcgaagccg tgtttaatat aattttcaac aaatggctgt aaagaaagtt
     1801 ttggtaacat taattgatca atatgatcaa tcagctgata gtcaaagtta tgtcgataac
     1861 gacgttgatg tagttgcaag gagaggtcga tattaattaa ttcctctttc aaatcaatta
     1921 tgtcgggctg atatgtatta ttgcgataca acttgctcaa taaatatata ctgtcgctga
     1981 tttcttcgtc atcattcaat atggcagtca ttcgaataaa ttccaaatta ttaaatagaa
     2041 aatgaggctc gatttgatat tgaagtgctg catactttgc ttcttgcttc aaccaattat
     2101 tttcataaag tgttttttga tatgtagcat tatctaaata aaactgattg atatgcatag
     2161 cgatttcttt caaatcaccg actttgtttt cgatattgat tgacttgtag tctcctgtag
     2221 atgaatgatt taaggaagta atgatttcct gatactgctg gatatagtta cgaaaacttc
     2281 tatttaataa ttgatgaata gcaagaataa acaaactcat taataagaaa atcatgattg
     2341 ccagacctaa taaggtttgt gacgaataat ttggattttc atatacttgt aatgtgtggt
     2401 taccgatagc tatctgttgc gtttttttcc atttagaagg cgagatgtct tcagataaat
     2461 tgacggaatc aataccatta gacaaagaat aattgtaatg tgttggaaag agactctttt
     2521 ttagcgcgtt gatcgaaacg tctagaatca atgtagcatc gggcatgtcc tcactaatga
     2581 aacttaatga acgaataaaa gaaatttttt cgttttttgt aggtgcgtag gcacttaagt
     2641 atttttcgta atctaatgag atagatatat ttttaacttc atcatcttgc acaagttttt
     2701 ttaagttatc tactaaatag gtaggggagc gatcctttga caaaatatct tccatgtatt
     2761 cagcaggtga tgagaggtag tagtcattaa ggttttcttg gaaatcataa ttttgataga
     2821 tctctgtcac gataccggag agaatacgat tatatgtttc catatagtgg gtgatttgct
     2881 gaatgttttg caggttttga tttttttctt tttcaatcac attgatatag gaaaagaaca
     2941 atgtgaaaca ggcaagtaaa gtaataaaaa aaatcaatat gtatgtaaaa cgcataataa
     3001 cttttgataa atcgtttttc at
//
LOCUS       DAZKCZ010000158         3011 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000158 DAZKCZ010000000
VERSION     DAZKCZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.162"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..382)
                     /locus_tag="ACG8KN_002480"
     CDS             complement(<1..382)
                     /locus_tag="ACG8KN_002480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189479.1"
                     /translation="MTIYLEENERIFEGKELYLIRNFSRKGIGFFEESGFYPDFIMWY
                     FNGSQEYIIFIEPHGMLRENLDGNKVQLYKEIKKVENEISLSEGTRPVLESFILSPTP
                     FATLNNPINNPSNYSKGDLNRQHVI"
     gene            complement(531..1046)
                     /locus_tag="ACG8KN_002481"
     CDS             complement(531..1046)
                     /locus_tag="ACG8KN_002481"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HFP9189480.1"
                     /translation="MVPLTSEKIFGLAPIIDDQAKILILGSMPGKESLKKEQYYANKR
                     NQMWKIMENTLKLQLPEEYPKRINVLRKHHIALWDVIHSCKRVGSLDSAITEDVPNDF
                     GSFFKEYSSLKIVGFNGNKAYQTFKSKIGFQDFPEIQFIKLPSTSPIPGKNVKSFEEK
                     VVEWSKVVNSL"
     gene            complement(1355..1717)
                     /locus_tag="ACG8KN_002482"
     CDS             complement(1355..1717)
                     /locus_tag="ACG8KN_002482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189481.1"
                     /translation="MADNLLNKNKGKRNLLERKEEVKPQQSFNRSSLFTVNESQQTNG
                     DQKKDVPRKKERGTTTTIRCATDTSHRLNAIVTSFNLDSVNELIEILLDNYEASLTTD
                     ERREIKTLLEVYQRKSKK"
     gene            complement(1759..2595)
                     /locus_tag="ACG8KN_002483"
     CDS             complement(1759..2595)
                     /locus_tag="ACG8KN_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9189482.1"
                     /translation="MAKVITFGNFKGGTGKTTNSAMMAYTLSKLGYKTLLADLDPQAN
                     ATSLYLHTKQRITNNIVTFDKTLMSAIADEDLSDIIIEIKENLFLLPSFADFTSYPLF
                     LEKKFPDSQFNRVTFLNELMTKIKDDYDFILVDVPPTLSTYTDSALLASDYTIIVLQT
                     QERSLVGAEAYVGYLQELIDNYNADFDILGVLPVLLKNNSKVDEATLNTAKEKFGEEN
                     LFENVVKNMERLKRYDIIGIVDPDLDTKYDIHDKRVMGLYKRVTEEMLKRLEEIESRN
                     KQ"
BASE COUNT          942 a          545 c          449 g         1075 t
ORIGIN      
        1 atatgacatg ttgtctgttt aagtcacctt tagaataatt agatggatta ttaattgggt
       61 tatttaatgt agcaaatggt gtgggagata gaataaagga ctctagaact ggacgggtac
      121 cttctgacaa tgaaatttca ttttctacct ttttaatttc tttgtataac tggaccttat
      181 taccatctaa attctctcgc aacataccgt gaggctcaat aaatattata tattcctgac
      241 tgccattgaa ataccacatg ataaaatcgg gataaaaccc agattcttca aaaaatccaa
      301 ttccctttct tgagaagtta cgaattaagt aaagttcttt accctcaaaa attcgttcat
      361 tttcctctaa gtagattgtc aagtctttaa taaatttcca ttcactatca actaacgcgg
      421 tagggactac ttgaatatcg gttagtcctt gttctacatg tatcaacggg ttatataaag
      481 atttatgttt cttttagtag gctctgattt tcaaatcaat acctaaatgt ttataagcta
      541 tttactacct tgctccactc tacaactttt tcttcaaacg attttacgtt tttccctgga
      601 attgggctag tagaaggtaa cttaataaac tgaatctctg gaaaatcctg gaaaccgatt
      661 tttgatttaa atgtttgata tgctttgttc ccattgaacc caacaatttt taaggatgag
      721 tattccttaa aaaaagaccc aaagtcattt ggcacatctt ctgtgatggc agaatctaaa
      781 ctccctactc tcttacaact atgaatcacg tcccataaag caatgtggtg ttttcttaga
      841 acattaattc gttttggata ttcttcaggg agttgtaatt tgagggtgtt ctccataatt
      901 ttccacattt gattcctttt gttagcataa tattgctctt ttttgagaga ttcttttcca
      961 ggcatggatc ctaaaatcaa aattttagct tgatcgtcga taataggagc taaaccaaat
     1021 attttttctg aggttaatgg aaccatattt ttatcccctt tgtagttcag tcacataaga
     1081 gttaattttt taaaacgtta tggtatctaa tccttgtagt atactagaat gtttttttta
     1141 gctttttttg gtgtctattg acgactttat cttcgtatat ttgaactaaa tcctcaaaac
     1201 gtttggtgtc actattgttt aaattttcaa cgtataagtt tacaaaagtc cttcccattt
     1261 ttttgactac atcattgtaa ttgccattat atagcgaaat ttacacgatt aaagagacac
     1321 gtctacatgt agacgtgtct acaaatctac aaatctactt tttagatttt cgctggtaaa
     1381 cctctagtaa agtctttatt tctcttcgtt catccgtcgt taaagaagcc tcataattat
     1441 ctaggaggat ttctatcagt tcattaacac tatctaaatt gaaagaagta acgattgcgt
     1501 tcagacgatg tgaagtatcc gttgcacaac gaatagtagt tgtcgtgccc ctttcttttt
     1561 ttcgagggac gtcttttttt tggtctccat tagtttgctg agattcgttt actgtaaata
     1621 aactggatcg attaaaagat tgttggggtt tcacttcttc ttttcgctca agaagattcc
     1681 gtttcccttt atttttattt aataaattat ctgccatact atcaccctct tctttcttgt
     1741 atatggtatg aataatgtct attgtttgtt tcgagattct atctcttcta atcgtttcaa
     1801 catctcttca gtgacacgct tataaagccc cattactctc ttatcgtgaa tatcatattt
     1861 agtgtctaaa tcaggatcaa ctatcccaat aatgtcatat cgttttaatc gttccatatt
     1921 tttaactaca ttttcgaaga gattttcttc accaaatttt tctttcgcag tattgagcgt
     1981 agcttcgtcg acctttgaat tgttttttag taatacgggt agtacaccta aaatatcaaa
     2041 atccgcatta tagttatcaa tgagttcttg caagtaccct acataagctt cggcccctac
     2101 caaagaacgt tcttgcgtct gtaggacaat aatggtgtag tcggaagcta acaaagccga
     2161 gtccgtataa gtacttaaag taggtggaac atcgaccaaa ataaaatcgt agtcatcctt
     2221 gatcttagtc attaattcat ttaaaaaagt caccctatta aattgactgt ctggaaattt
     2281 cttttctaaa aataagggat aagaggtaaa atctgcaaag ctaggcaata aaaaaagatt
     2341 ttctttgatt tcaataataa tgtccgataa atcttcatct gctatggcag acattagcgt
     2401 cttgtcaaaa gtaacaatat tattcgtaat tcgctgtttc gtatgtaaat acaaagatgt
     2461 tgcattcgct tgaggatcta aatccgctaa taatgtctta tagcccagtt tagataatgt
     2521 gtaagccatc atggcactat tagttgtttt acctgttcca cccttaaaat ttccgaatgt
     2581 tataacttta gccattgtaa ccctcttttc ttagttgtct acaaatctac atttgatatt
     2641 ataacgtaaa tataccatgc taaatcaata tgtcaaacgt tatcaagaaa aaccgtctac
     2701 aaatatacat gtagacgtat ctacaaatct acacgtctac atgtagacgc atatacatta
     2761 aaaatcttga taacaaaagg atatcaagca tcatgtatat gcatctacaa atctacaaat
     2821 ctacatgtag acgtgtatac aaatctacaa atctacatgt agacgcatct acagaaaaac
     2881 gagattttgc ctacaaatta aggttttaca aaaaaaaaat agtgtagtac atttgattta
     2941 attcaaagca aaactaaaat tgaatacaaa aaaagccccg tagacgccaa tctacgagac
     3001 acgcaaaact t
//
LOCUS       DAZKCZ010000159         2984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000159 DAZKCZ010000000
VERSION     DAZKCZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.163"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..473
                     /locus_tag="ACG8KN_002484"
     CDS             27..473
                     /locus_tag="ACG8KN_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189483.1"
                     /translation="MQQNRIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNKKM
                     SLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVS
                     YKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            487..1176
                     /locus_tag="ACG8KN_002485"
     CDS             487..1176
                     /locus_tag="ACG8KN_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189484.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1301..>2984
                     /locus_tag="ACG8KN_002486"
     CDS             1301..>2984
                     /locus_tag="ACG8KN_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFP9189485.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVT"
BASE COUNT         1001 a          403 c          628 g          952 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg tattttttca gtatttacaa
       61 tattaattgg gggctatttt cttttgaatt tagattctgt taattcaaac gctcaaataa
      121 aagatcaaag agtagctgaa cataataata aaaaaatgag tttgaccaaa aatatctatt
      181 ctatgtcaga tgaagaattg tataaagaat ttggtgattt atccggggaa cagttagaag
      241 aaagtattcc aggaattagt gactcagata aaattgttgt cttgtctgct ggtgggcaat
      301 taatgggaga tggcaaatcc gaattagtta gttataaaca aaatcacaga cagtttattg
      361 atgttaatac tgattccaac gctatttcga ttaaggatct aaaagagctt ttaaatagta
      421 aaagaaatga attaaagcag ataaaagaaa ctaattcagt ggaattagag taaaggaaga
      481 ttcattatga aaaagatttt tggtgtttct gttgggctac ttatgatgtt aacatttgga
      541 aatgcagtat atgctgctga gaaagaagtt atttcagatc cagagcagta cgcctcacga
      601 tactttgctg attttgaaga agaagataaa gtacttgcat tgccagaagg tggtttttta
      661 aaaggggaag ctaaaattgt atctatggat aatccagata taattcaagg ttcatataat
      721 tcagaaactg atcctgcttc agtttcagtg acagaagcaa aaaaaattgt tgctgaaaaa
      781 ttcaaaaatg atattgaaac tactccaatt atgcctcgag gagcatttcc acccgatgtt
      841 ggtactagcg gacaccttct tgtactagca ggaggaggaa gctataaatc acagccattt
      901 agtggtgcgg gatggcgttt tggaggtatg ggtgtaaaac cagctgatgg tacaggcaac
      961 tttttacgtt ggaaatccta cattgacgat ggaagagtag gaaatgtaaa tcaagccaac
     1021 gcaactaaat ctggttctat tcaaggaatg gcattgtatg ctcgtgaagg attcaaatac
     1081 tttgcaccaa acgggcctgg ttggacaatg aatataggta tttattacac ttataatcct
     1141 gttgcaggta cgtattatgt tgttcaaaat atttaataaa acagcacaaa gagatttctt
     1201 tgtgctgttt tattattttt agttgttgac aaacactaaa atataaaata taatcaacac
     1261 ataaacgaac gtatgttcgg caagaaagca ggggattttt atggatgaga tggttttaag
     1321 tactcagaaa tggctaaaca agaaatacag taatgttaca gggtttgata aggttccaga
     1381 aaatgggcga acagggtggc ctacaatcta tggacttatt gaaggactac aagtagaact
     1441 tggtattact aatctagtag ctaattttgg acctaccact gaaaaaatgt acgataatca
     1501 ggttactcct aaatggggca aaaatctacc taaaaatatt gtatttctta ttcaaggtgc
     1561 cttttggtgt aaggggatta atccaggtgg cttcgatggg gtatatactc catatttaga
     1621 tactgctgta aaagagttgc agactgatgc agggtttaat ggaagcgctg taactggagt
     1681 tttggatgcg aaatgggcca aagcgttatt tgatatgagt gcttttgttc ttgttcccgg
     1741 cggagatagt aaaattagaa aaatgcagca atggttaaat gtaaattatt tagaatacac
     1801 gggaataatg ccttgtgatg gtatttatca aagaaattca aatcaagcat taatttttgc
     1861 attacaagct gaactaggat attctcccag tgaagcaaca gggtcttatg gtaatgggac
     1921 aactagtaaa acttatcctg tgtctgaagg taactcggga aactatgtaa gaataattca
     1981 atatggatta tatgttaatg gttattttga agacggtaca ttcgatggta ttttcactaa
     2041 atatatgggt ttagaagtac tggcattccg aaaatttatg gtattgcctg aatatactga
     2101 aatagcagat gttgttgtaa taaaaggact attgtctagc gctggagata ttagaagatc
     2161 tgcagatggc gcagatactt ctacgcaatt aactagatct caaatccaaa cattggttga
     2221 taatgatata aaaatagttg gtagatatct tactggtact gttggaattg gaaaagatga
     2281 acgaaataag tatctaacta cagaggaatt gaataatatt ttttctaaaa atttatctgt
     2341 gtttcctatc tatcaagatg gaggtgcagc attagcttat tttacttatg atcgtggctt
     2401 atcagatggt aagaaggcta tagatgcagc aaaaaatcta aatataccat taacaacagt
     2461 tatttatttt gctgttgatc tagacatgtt aggggaagaa attcttgcat atgctggaga
     2521 atattttaag ggggtatctg ctgtaatgtc atatagcggg tatcaaacgg gtgtttacgg
     2581 aacaagaaat gtttgttctc aaataatcaa taacgggtat gcttcgtttg catttgtttc
     2641 agatatgtcg actggttata gtgggaattt aggttttcct atgcctagac aatggtcttt
     2701 tgatcaattt gttgaattta ccataggaag tggcaacgga gctgttggta tcgatttaat
     2761 cgctacatcg ggtagagata gcggatttaa tgagttatca aatgatacta ataatgataa
     2821 ttacattgcc aaatacaatc agaaggttat aaatcagatg gtacgtgcgt atcaatatct
     2881 atcacaagct ggtcttgata atccttggaa ccctcatttg acattttata gatatgtaaa
     2941 ttattctggt ttgtcatggg acatcatttc ttctcctgta acag
//
LOCUS       DAZKCZ010000160         2974 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000160 DAZKCZ010000000
VERSION     DAZKCZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.164"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..208
                     /locus_tag="ACG8KN_002487"
     CDS             <1..208
                     /locus_tag="ACG8KN_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP9189486.1"
                     /translation="KRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKEVM
                     EIRLRSKLKSVLIVHYLKILYNRN"
     gene            272..325
                     /locus_tag="ACG8KN_002488"
     CDS             272..325
                     /locus_tag="ACG8KN_002488"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFP9189487.1"
                     /translation="MRNVDKTSTVLKQKSSP"
     gene            complement(363..1535)
                     /locus_tag="ACG8KN_002489"
     CDS             complement(363..1535)
                     /locus_tag="ACG8KN_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFP9189488.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            1553..1672
                     /locus_tag="ACG8KN_002490"
     CDS             1553..1672
                     /locus_tag="ACG8KN_002490"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFP9189489.1"
                     /translation="MFLWLKTLYRKRAVTWPFIFYTIIWTLSKQTKNSDYADK"
     gene            1797..2534
                     /gene="erm(B)"
                     /locus_tag="ACG8KN_002491"
     CDS             1797..2534
                     /gene="erm(B)"
                     /locus_tag="ACG8KN_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP9189490.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            2704..2964
                     /locus_tag="ACG8KN_002492"
                     /pseudo
     CDS             2704..2964
                     /locus_tag="ACG8KN_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT         1001 a          530 c          536 g          907 t
ORIGIN      
        1 aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata atgggagata agacggttcg
       61 tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt
      121 aaaagaagtt atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcatta
      181 tcttaaaatt ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa
      241 gaaggaggga ttcgtcatgt tggtattcca aatgcgtaat gtagataaaa catctactgt
      301 tttgaaacag aagtcaagtc cataatcctg tgtaaaatac tatacaatgt tttaccggtc
      361 tctcattgat caaacttaat atattttctt gtagaactga gccattcgtc atgaaggtcc
      421 ataaggacag ctccaattaa tcgattggca gacgttcggt tgggaaaaat ccgaataatc
      481 ttttctcttc tgcggacttc ctggttcagt cgttcaagaa ggttggtgct ttttagccga
      541 ttatgactat ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt
      601 tcgcaggctt ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct
      661 gttcgagcga gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt
      721 tttgaattct tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc
      781 tgccaacttg cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca
      841 gaaatgatga gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac
      901 catgtgtcat cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct
      961 tctgttatcc cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact
     1021 tttatgtaga gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc
     1081 tgccattcgt tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat
     1141 acagattttc cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa
     1201 acatacatct caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac
     1261 accgtcggtg aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca
     1321 cgagtcgtaa agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt
     1381 tcatagtcat cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca
     1441 gtggttaaaa tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc
     1501 tcttcgttgt tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt
     1561 gtggttaaaa acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat
     1621 tatatggact ttatcgaaac agactaaaaa cagtgattac gcagataaat aaatacgtta
     1681 gattaattcc taccagtgac taatcttatg actttttaaa cagataacta aaattacaaa
     1741 caaatcgttt aacttctgta tttgtttata gatgtaatca cttcaggaga gattacatga
     1801 acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc aaccaaataa
     1861 taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca ggtaaagggc
     1921 atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa ttagacagtc
     1981 atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact ttaattcacc
     2041 aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt gggaatattc
     2101 cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccgt gcgtctgaca
     2161 tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac cgaacactag
     2221 ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca gcggaatgct
     2281 ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat accacagatg
     2341 ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc aatcgagaat
     2401 atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca
     2461 atttaagtac cattacttat gagcaagtat tgtctatttt taatagttat ctattattta
     2521 acgggaggaa ataattctat gagtcgcttt tttaaatttg gaaagttaca cgttactaaa
     2581 gggaatggag ataaattatt agatatacta ctgacagctt ccaagaagct aaagaggtcc
     2641 ctagcgccta cggggaattt gtatcgataa ggggtacaaa ttcccactaa gcgctcggga
     2701 ccccttgtag gaaaatgtcc taagtgtggc aacaatattg tattaaaaaa atcgttttat
     2761 ggttgttcaa attatcctga atgtaagttt actttagctg aacattttag aaagaaaaaa
     2821 ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag aaaccctggt aaaaggaatc
     2881 aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt
     2941 gattttatat ctttctcaaa ataaacataa aagc
//
LOCUS       DAZKCZ010000161         2918 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000161 DAZKCZ010000000
VERSION     DAZKCZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.165"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1361..1879)
                     /locus_tag="ACG8KN_002493"
     CDS             complement(1361..1879)
                     /locus_tag="ACG8KN_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFP9189491.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2246..2494
                     /locus_tag="ACG8KN_002494"
     CDS             2246..2494
                     /locus_tag="ACG8KN_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189492.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT          968 a          528 c          413 g         1009 t
ORIGIN      
        1 ttttacgttc tattcagatt aacgggtagg cttactgtct tttttggcaa taattttgca
       61 atcaagccat ttacagatgt aatgcgcgga tacttctgct aagattgcaa aaaacatatc
      121 gaaaaggtaa ttcataatac aacctcctcc ccttgccata ataggccggg gtagcagaca
      181 gcataaatat tataccataa ctgaaaattt cattttagta catttcaata tattgatgaa
      241 ccagcacaat aaaacatgcc atgactaaca gaaaaaaact tattaaacca gatataactt
      301 gtaaacatcc aactatatag aaaacgtaaa ttttctatat agttggatta agataacgac
      361 tatttatttt atcatatttt tactataccc tatactttta cttggtatat ttatgaataa
      421 tcgcaaattt acccctatac tactagtttg taagttagat taataaaaat actaaagtta
      481 tttgttcttt tattaaatac acctacaact tataaattga tggtatttag ataacacgaa
      541 agaataaaaa taacagctga tagtaaagtt tgttgacgat tgctactcaa tcgaaagtga
      601 gttagtgcgt atgaacttgc ttctgaaaac ttatgaaatg aaagaccttt tacatcttgc
      661 agaaatcggt actgggatta aactaagcaa taaaaactaa tgttcccctc attctttgaa
      721 cttctactat ttgatttatt tattctgata aatgacgatt tcaccttcag gatcttcaaa
      781 tcccgtatag aatttattta agatctcttc agtatcagta ataggatagg cgataggcca
      841 catacccttt gatggttaga ataaactgct caaaatctgt atcgtatgtt ggctttagca
      901 tattaacaaa ggctatctcc aaattataaa ccataagcgt tgaatcgttc ttaacaattt
      961 caattcttcc taacccaaag tcagaagata taattaccgg acttgcctta cccttcatgt
     1021 gggttgttga gattccatat ttaaagggaa ttgtcggtac tatcggcggt ctgaaaaaca
     1081 tcccaaaaat aaaaaaatct ttcccctgct agtctcctaa gtccctttgt agcggatatg
     1141 gcaccataat ctcattttat cgaagtgagt catggtttat ctgttggcca accaaaattg
     1201 atacaatcta acgatgcacc ctctacggca ttctagggga gtgcacttgc attcacccaa
     1261 taaaaaccgg cagttagcaa agaacttatg tagtcgtatg ttcttactgt taacatacaa
     1321 gaggtctcct ttccacaatt gcttagcagt aaacgggaat ttacaaagct aatctcttcc
     1381 attttcttat ctttgttcta aaagtcccaa atggagcaac tgtatttaca tgaataaatt
     1441 tatatacttc ccatacagcc gttttggttg cctcgtctgc ccattttctt ttgtgtgcag
     1501 tgaatagatc ttcatcactc attgaatcaa tcatcatgtt aatatcatca atattacgat
     1561 tgagaatttc ctctaattct ttcaatggta gatgtgcata ggtatttgta aaccacttat
     1621 ataactctcc taactgattc catttaaatt gatcagaagg cgttttaaca gataatccat
     1681 ttctttcatc attttcccat tttagaatta aattcgtcca accaacttga tatgctaaat
     1741 tttcagcagg tgttctatcc acttcctcaa ctcttttatc cttcaattct tcaggaatag
     1801 tttcaaattc taaaatatat ttagtataag tcctatttat ttcttttttt aattcttctt
     1861 tatttttata tgttctcatt atattacctc cataaattcc taattgtctc ctttaattca
     1921 tctataattt catccacgat attttaatcc gttctacaat gcatatctga tatgcattga
     1981 ctgtctaact catatgtgaa tgatttataa aatatcccaa cgtctcatct aaagaaatgt
     2041 tgatcgtcag caaggagcta aaaaatacga atatatttct tttgtagtaa aatcggactg
     2101 gtttgcatat cattttcttc gtaaatatca aaatcatgtt aactactatc aattactaat
     2161 aaaaactacc actttaagcg ttaagaactg ttaagtttag ttaagtgagg atagttcaca
     2221 aaatccattg ttaaacttca cgaaagtggt aaaactcaaa atgctttata taggattaca
     2281 gcatcgctct ttcttcgatt gcgcgttggg taaaaaatat tatgaagtaa aaattgacga
     2341 tgacacgttc cttacggctc atcagatgaa acagctacaa aaacgaaatg atcaactcga
     2401 agaaaattta attctaaaaa agcgattgcc atattcacgc ctcgctcgac gaactattaa
     2461 aggtcaagat ttacctgaag atcatccttt ttaggaaatt gaagcttttt atgttgcaca
     2521 atgcatttat aatactacaa tgatgttttc accagatatt cttctagggt gcggtgtaat
     2581 gaaacagata cacttgcaaa agaaaattca ccgcgaattt actaaaatta tgaataatta
     2641 tgttaatctt ccgaaatttt tttccaaaat taagggatca agctggtgtt atagattgtt
     2701 taaggttggc aaaaaaataa cttcttttta aacaattata gaaaaccgat actctgttaa
     2761 gttggttgac acggtgtcga tttttatgac tttcttattc tgaaacttca ctaacggaca
     2821 gtacctaaaa agtctatagt atggacgttt acgagtacta ttttatcaaa aatgctgctc
     2881 atttcaactt atatgtttta gtgaactacc cccactta
//
LOCUS       DAZKCZ010000162         2852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000162 DAZKCZ010000000
VERSION     DAZKCZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.166"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..882
                     /locus_tag="ACG8KN_002495"
     CDS             67..882
                     /locus_tag="ACG8KN_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189493.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1008..1265
                     /locus_tag="ACG8KN_002496"
     CDS             1008..1265
                     /locus_tag="ACG8KN_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189494.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1517..2089)
                     /locus_tag="ACG8KN_002497"
     CDS             complement(1517..2089)
                     /locus_tag="ACG8KN_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9189495.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2105..2710)
                     /gene="fic"
                     /locus_tag="ACG8KN_002498"
     CDS             complement(2105..2710)
                     /gene="fic"
                     /locus_tag="ACG8KN_002498"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFP9189496.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          922 a          524 c          447 g          959 t
ORIGIN      
        1 aaggtgctgg actacaagta aaaaatactg tatgatcatg acagattcaa gaaaggagca
       61 ataagattgg ttaagatagc aggactttct aaaggtggaa aaaccgtttc acaactttat
      121 aaacaaaact acagagactg gagacagttc agaaaaaaca ttaaggattc ttactttatt
      181 ctaccaacag aacttgaaaa atatttaccg aataacaaag ctattaattt atatctgtat
      241 tactgtttta aggcaaagaa tgaaggcggg gattcctggc attcggtcaa taccatagca
      301 gagtctctag gagtgacaac taaaactata aacacctgga ataaaacctt agttgattta
      361 ggtatgattg cacgtataga cgataaccat ctcagtaaat cgacaatttt acttcctctc
      421 tctagttact ataaattagc ccttgatagt tctttaaagg atgttaccga gagcactgtc
      481 catgaaattg atggcaactt ggttgcagtt tatcatttgt ttgaatggag aaaaaatgag
      541 gctgatgaaa attataacgt tccttataac acactctgtt gtgtttttaa aagaaaaaca
      601 caaaaagata ctatctataa atttatcttg gtccaagatg agaaaataag taatttcgaa
      661 ttaagcactc cttctagtaa gatttatgac gatgcatata gatttgaatg tcaagatcta
      721 gaagccctcc tttccgcaaa taatctaaac aatatagaac atgaaaactt tgttgtaaac
      781 actagattta atttagtttc tgaaaaggac tcagatttgt tagatttgct gatagagctt
      841 actaaaaatc tagataagaa agaaaacatt actgtactat aactagcatc ggtccatcat
      901 taagtttgga ctattagaac acttgctatt acagaaaaag gtatgctagc cacgcttact
      961 atttattggt atcaaacaag tatcttaatt tgaaaggaag atgatttatg gctaatttat
     1021 atatagagaa caaaggagac ttgaatgcca ttattacttt aatcgtagtt atggtaaaat
     1081 caataggtaa tatcacaata ggaatgctgt ttattatatt agcagcagtg ctgattttta
     1141 tgatagttat ttggttctta aatgaaagat ctaaagacaa tcaacaccgt agggatatgg
     1201 agataaaatc aatagagtca atagattatt tatcgataat aacactaaaa aagccacgtt
     1261 cctaaacgtg acttacgctg aacgatatgt gttgtttggt agacgaacaa atcgttcatt
     1321 ataaatatat gatggtatcc taatatagta agcgtggcta gcataccttt aattacttta
     1381 ccttttacag ttaccataca cattttttga gcagaaatca agtaaataaa aaaatattat
     1441 atttcacaac atgctaatag actcatatct agaacgcatt aagtatttac ctgtctggtt
     1501 cgctgagact tcttttttaa ctttcttcaa ttttcttctt tattctaaaa agcgtactgc
     1561 gactgaaacc ggttatagct tcaacttcct tatacgattt acctgaagta agcagttcat
     1621 atgcgtgcct cttcttcggt gtgattgttg ctttcggacg tccttctctg aaattaggat
     1681 tattctttct tgcaaacatt tttccttctt gggtacgttc cacaatcata tcacgttcca
     1741 tttctgctac actcaacaac gtccgtgtca ccattcgccc catggacgta ttttcaatcg
     1801 ttcctagatt caaaaccttg atcgttacgt tatcatcgag taaaggttca atgatctcta
     1861 atgcttctct cgtgttacga gcaaatcgat caagcttcgt aacaactatt tcatctccag
     1921 gcttcagacc agccaacagc tgattgaatt ttggccgatc ccgttttgta cccgtaaatt
     1981 tttctgaaaa aatattcttc ttctctacgc cgttctcaag caactgttga atttgttctt
     2041 ttgtactttg agaagcggtt gagacacgtg catatccata tttcgtcatt tggtccacct
     2101 acttttacaa atcatctatc gtatactcag aatacccttc atagaaatag ctgacatcga
     2161 ttcctttcat atataactcg cgatcgtcaa ttttatcagt cagagcattg gaaattagat
     2221 agcgtatttc caaatcgttt atgggacttc gctccattgc agaaagatag tctgctttat
     2281 cgattaagtt ccaatctaca actttttgca attcttcctt taaaatgaga tccagccaaa
     2341 ttctagtact tcggccattt ccttctcgaa atgggtgagc gatattcatc tccacatatt
     2401 tcttgataat ctcttcaaaa gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt
     2461 ctaaatacat aactggtgca aatcgaaaat ttccttttgc aatattgacc gatcggattt
     2521 ttccggcaaa atcgtaaata tccgaaaata gaaattcatg aatttcagat aaacctttaa
     2581 atgtaccaac ttcgatttca tttatttttc cacaatcgta caattctttc gctttttgtt
     2641 tactgagttt ttcttcaacc ttagccaact caacttgatt ggttaatccc aatttattct
     2701 ctaaaatcat gcgaaatctc cttaacttta tgacataagt ttatgaaaca ctctcaaccc
     2761 cattatatcg cgattttgtt tatgtgtcaa aaactattaa gtttctgacc cccgatagat
     2821 ttttcaagtg tctctatttt gcacagtcta gt
//
LOCUS       DAZKCZ010000163         2846 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000163 DAZKCZ010000000
VERSION     DAZKCZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.167"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..900
                     /locus_tag="ACG8KN_002499"
     CDS             4..900
                     /locus_tag="ACG8KN_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9189497.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            992..1207
                     /locus_tag="ACG8KN_002500"
     CDS             992..1207
                     /locus_tag="ACG8KN_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP9189498.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1225..1497
                     /locus_tag="ACG8KN_002501"
     CDS             1225..1497
                     /locus_tag="ACG8KN_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9189499.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1499..2362
                     /locus_tag="ACG8KN_002502"
     CDS             1499..2362
                     /locus_tag="ACG8KN_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9189500.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1131 a          450 c          498 g          767 t
ORIGIN      
        1 ttaatgatac aatattacta tacaaaaaaa gaatggggcg tagttatgga gaaggaaaaa
       61 ctaaaaatac ttgaagaatt aagacgtatt ttaaacaata aaaatgaagc aattattatc
      121 ttgaataatt actttaaagg tggtgttgga aagtccaaat tatcgactat gtttgcttac
      181 ttgacagaca aattgaattt aaaagtttta atgatcgata aggacttaca ggcaacattg
      241 acaaaagact tagcaaaaac ttttgaggta gaattgccac gtgtcaattt ttatgaaggc
      301 ttgaaaaatg gaaacttggc ttcttctatt gttcatttga ctgataattt agacttgatc
      361 cctggcacgt ttgatttgat gttactgcca aaattaactc gctcatggac ttttgaaaat
      421 gaaagtagat tgcttgctac tcttttagca cctttaaaaa gtgactatga tctcattatt
      481 attgatactg taccaacgcc aagcgtttat acaaataatg caatcgtggc gagtgattac
      541 gttatgatcc ctttacaagc agaagaagaa agtacaaaca acattcaaaa ctatatttcc
      601 tatttgattg atttacaaga acaatttaac cctggactag atatgatcgg ttttgttcct
      661 tatttagttg atacggacag cgcaacgata aaatcaaacc tggaagaact gtacaagcaa
      721 cataaagaag ataacttggt tttccaaaat attatcaagc gaagtaataa agtaagtacc
      781 tggtctaaaa atggaattac agaacacaaa ggctatgaca aaaaagtttt atccatgtat
      841 gagaacgtat tttttgaaat gcttgagcga attattcaat tagaaaacga aaaagaatag
      901 aatcacaaat cacaagtgat taatcacaaa tcacttgtga tttgtgattg ttgatgataa
      961 aataagaata agaagaaata gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa
     1021 agcaaaacga aatgatacac caatcagtgc aaaaaaagat ataatgggag ataagacggt
     1081 tcgtgttcgt gctgacttac accatattat aaaaattgaa acagcaaaaa atggcggaaa
     1141 cgtaaaagaa gttatggatc aagccttaga agaatatata cggaaatatt tacctgataa
     1201 actttaaaaa ggagtgaaaa tgaaatggca gttacgtatg aaaaaacatt tgaaatagag
     1261 atcattaacg aattatcggc aagcgtttat aatcgagtat taaactatgt tttgaaccat
     1321 gaactagata ctaaaaatac tcgtttacta gaagtgaatc ttttaaatca attagaagtg
     1381 gcacaagaag ttgatttatt tcaacaacca tttgaagaat tacaagctat tcatgagtat
     1441 tggcggtcaa tgaatcaata ttcaaaacaa attttgacaa aagagaaagt ggcttaacat
     1501 ggcgaatatt actgacttca ccgaaaagca atttgaagat cgtttagaaa agaatgttga
     1561 acgactaact aaaaatagac tagcggttga atcgccaacc gcttttttac ttggtgggca
     1621 accaggatca ggtaaaacta gtttgcgatc ggcaatttcc gaagagacac aaggaaatgt
     1681 tgttatcatt gataatgata cgttcaaaca acagcaccct aattttgatg aactagtgaa
     1741 actttatgaa aaagacgtag taaaatacgt taccccttat tctaatcgca tgacagaagc
     1801 gatcataagc cgtttgagag ataaagggta taatttagtg attgaaggta caggacgaac
     1861 aacagacgtt cctattcaaa ccgcaacaat gcttcaagcc aaagattatg aaacaaaaat
     1921 gtatgtcatg gcagtaccta aaatcaactc atatttagga acaattgaac ggtatgaaac
     1981 tatgtatgca gatgatccaa tgacagccag ggcaacacca aaacaagcgc atgatattgt
     2041 tgtcaaaaac ttaccgacca atttagaaac ccttcataaa acgggcttat ttagcgatat
     2101 aaggctttac aatagagaag gagtgaaact ctattcaagc ttagaaacgc cttccattag
     2161 tccgaaagag accttagaaa gagaattaaa tcgtaaagta tcagggaaag aaattcaacc
     2221 gactttggag agaatagagc aaaaaatggt tcaaaatcaa caccaagaaa cccctgaatt
     2281 taaagcaatt caacaaaaaa tggaaagctt gcagccacct acaccaccaa tacccaaaac
     2341 acctaaactt ccagggattt aaaccttaaa aaagtaaaag agtagttacc aaaaaacggt
     2401 aactactctt tttttataaa aacatttcct cattttttaa aagctgatcg agaaataaag
     2461 cacacaactg tattctttct tttttcagtt ggtacatttt gcagtctgta acgtgccatt
     2521 tactgtttgt taccttaaca tatccagtcg aaaacacaag ggtatataaa cgagcaagct
     2581 cgcccttgtg ttttctttgt gtctatgttg cgttctcgtc acagcaaacg acacgaacac
     2641 actggcgaaa atgaaccaac aaaacgaaaa aagaaaggag cgtacactgt aatttatttc
     2701 tagcttttaa aacatgagga aaacagcttc acttcctcaa acgcctaaca aaaatggttg
     2761 tcaaaaaact aaaaatcaaa aaaagaaaag aggaaaagaa aatggaaaaa acaaaaaaat
     2821 tacaattaga agattttaca gaaaat
//
LOCUS       DAZKCZ010000164         2836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000164 DAZKCZ010000000
VERSION     DAZKCZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.168"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..254)
                     /locus_tag="ACG8KN_002503"
     CDS             complement(<1..254)
                     /locus_tag="ACG8KN_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9189501.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
     gene            complement(244..1443)
                     /locus_tag="ACG8KN_002504"
     CDS             complement(244..1443)
                     /locus_tag="ACG8KN_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9189502.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1573..>2836)
                     /locus_tag="ACG8KN_002505"
     CDS             complement(1573..>2836)
                     /locus_tag="ACG8KN_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9189503.1"
                     /translation="ATDRASKETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKI
                     SQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKET
                     KFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADE
                     TVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKG
                     DKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVV
                     VSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADK
                     LGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFM
                     SLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          865 a          551 c          431 g          989 t
ORIGIN      
        1 gtatcgactt ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac
       61 accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct
      121 tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga
      181 tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg
      241 tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa
      301 aagactgtag acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga
      361 gaacgctcct ttttttgtga cttttctgaa gctggagtgg acactttcaa ccgcattggt
      421 agtgtacata atttttcgaa tggcactacc ataatcaaat agttgttcaa catgagaaaa
      481 gtttcgtttg catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc
      541 gaaggcagcg tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca
      601 gacttcctta taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat
      661 acagcgctgc acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac
      721 accgtccatc gaaatgaaaa aaacatcttc tacaccacgt actttcagtt catcaaatac
      781 ttgcatccag cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt
      841 tcctttaaga tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc
      901 gcgtaaagtg acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg
      961 agcttgccat tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg
     1021 ggaaatatca aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc
     1081 tcgtgcatac atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg
     1141 aatcaactca ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc
     1201 aaagctcgtt ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg
     1261 ctctttagaa tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc
     1321 aaataaggga ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg
     1381 ataagtattc aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc
     1441 catcgtgtaa tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc
     1501 gcctaggatt gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa
     1561 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
     1621 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
     1681 catgaattta ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc
     1741 ttctgcccca ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc
     1801 accagtaacc ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa
     1861 ataggaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
     1921 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
     1981 catccataag aataataata atgaaactac aactcctgca aatattaatg agccaaaaaa
     2041 taatttattt gaaattgagt aaatacctga tatagattgt tgtaatgcag ggtaatttga
     2101 tgaactttta atcaaattat attctctcca atttatatct aatttcccaa gatcttttat
     2161 tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt
     2221 atcttcggta ttaccataaa ctttagcagc tgtatgaata tcagtgatta atgtattttc
     2281 gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat
     2341 ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt
     2401 tatttttatc ttatctccaa ctttaaggtt atttttttca gctaaatctt tatgcattaa
     2461 gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga
     2521 tacaaatttt gtttcttttg ttgagtcgtt aaccccagtt aacataactg ttcttttaaa
     2581 attctttgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata
     2641 atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg
     2701 agatatcttt ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt
     2761 tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg tttcttttga
     2821 agctctgtcc gtagca
//
LOCUS       DAZKCZ010000165         2797 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000165 DAZKCZ010000000
VERSION     DAZKCZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.169"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1169)
                     /locus_tag="ACG8KN_002506"
     CDS             complement(75..1169)
                     /locus_tag="ACG8KN_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFP9189504.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="ACG8KN_002507"
     CDS             1358..2119
                     /locus_tag="ACG8KN_002507"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9189505.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="ACG8KN_002508"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="ACG8KN_002508"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9189506.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          892 a          554 c          469 g          882 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag caatcttttt
     2761 ttaatttaag aaaaaatagt ttgacaatca aaacatt
//
LOCUS       DAZKCZ010000166         2784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000166 DAZKCZ010000000
VERSION     DAZKCZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.170"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..1854
                     /locus_tag="ACG8KN_002509"
     CDS             76..1854
                     /locus_tag="ACG8KN_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFP9189507.1"
                     /translation="MTTFEIKEEFLLKGKTFKILSGAIHYFRIPPCDWEHSLYNLKAL
                     GFNTVETYVPWNLHEPQKGEFHFEGILDLERFLTIAQDLGLYAIVRPSPYICAEWEFG
                     GFPSWLLREPIHIRRNEIAYLEHVADYYDVLMKRIVPHQLNNGGNILMIQIENEYGSF
                     GEEKEYLRAIRDLMIKRGVTVPFFTSDGPWRATLRAGSMIEDDILVTGNFGSKAKDNF
                     NSMKQFFKEYDKNWPLMCMEFWDGWFNRWKEPIIQRDPQELAEAVKEVLEQGSINLYM
                     FHGGTNFGFMNGCSARGVIDLPQITSYDYGAPLDEQGNPTEKYYALRKMIHDNYPEIK
                     QLDPVIKPTIEKKKISLTNKVSLFATLDTISQPVQSKYPKTMEELGQNTGYLLYRTMI
                     ERDAEEERLRVIDGRDRSQLFLNQELQATQYQTEIGEDIQVSMTEEINQVDILIENMG
                     RVNYGHKLFAETQKKGIRTGVMADLHFITNWQQYSLPLENCKTVDYSKGWQPQHPSFY
                     RYEFLLEDIGDCFIDLRKFGKGIVFVNQTNIGRFWEVGPTLSLYISSGFLKVGKNEIV
                     IFETEGTYEPEINLLKAPLYKEMKEG"
     gene            1858..2337
                     /locus_tag="ACG8KN_002510"
     CDS             1858..2337
                     /locus_tag="ACG8KN_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9189508.1"
                     /translation="MSIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVAQNDIEK
                     SGLKLATPAGVKLSILPIEKAAQNILAGKYDSQRLLIIARKPDRLLKLVELGVPIKEI
                     NVGNMSQTSETRSITKSINVVDQDVETFNQLNEKGVHLIAQMVPSDKADDFMSLLNK"
     gene            2360..2767
                     /locus_tag="ACG8KN_002511"
     CDS             2360..2767
                     /locus_tag="ACG8KN_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9189509.1"
                     /translation="MAGHLVLISHGRFCEELKRSVDMIMGVQKDIYTVGLLPEESAKD
                     FRVNFEKTINGLKKVTVLADLMGGTPCNVVSQLILEGKEIELYAGMNMPMVIGFVNNQ
                     FLGEDSSLVNKGRENIIHVNEYLEVNPIDLEDE"
BASE COUNT          993 a          347 c          606 g          838 t
ORIGIN      
        1 aaaaaaattg ttgactttta atacaaaagc attaatatac taagtatatt aacacattga
       61 ggtgaaaagt aacagatgac tacatttgaa ataaaggaag aatttttatt aaagggaaaa
      121 acatttaaaa tactatctgg tgcaattcat tattttcgga ttcccccatg tgattgggaa
      181 cattctttgt acaatttaaa agcactagga tttaatacag tagagacata tgttccttgg
      241 aatttacatg agcctcaaaa aggagagttt cattttgaag ggatattgga tttagagaga
      301 tttttgacaa tcgctcaaga cttaggatta tatgctattg tgcggccatc gccttatatt
      361 tgtgctgaat gggaatttgg agggtttccg tcatggctac tccgtgaacc gattcatatc
      421 cgaaggaatg aaatcgctta tttggagcat gtagcagatt attatgatgt tttgatgaaa
      481 aggattgttc cccatcaact taataatggg ggaaatatat tgatgattca aattgaaaat
      541 gagtatggat cttttgggga agaaaaggaa tatttgcgtg cgataagaga tttgatgatt
      601 aagcgaggag tgacagttcc ttttttcact tcagatggac cgtggagggc tactttacga
      661 gcgggaagca tgattgaaga tgatattttg gttactggta attttggttc taaagcaaaa
      721 gacaacttca actctatgaa gcagtttttt aaagaatatg ataaaaattg gccactgatg
      781 tgtatggaat tttgggatgg ttggtttaat cgttggaaag aaccaattat tcaacgagat
      841 ccacaggaac tcgctgaagc ggtaaaagag gtattagaac aaggaagcat taatttatat
      901 atgtttcatg gaggaacgaa ttttgggttt atgaatggtt gttcagcaag aggagtgatt
      961 gatttaccgc aaattacttc ctatgattat ggtgcaccat tagatgagca ggggaatcca
     1021 acagaaaaat attatgcatt gcggaaaatg attcatgata attatccaga gattaagcag
     1081 ctagatcctg tcattaaacc cacgattgaa aagaaaaaga tttctttgac gaataaggta
     1141 agtcttttcg cgactttaga taccattagt caaccggttc aatcaaagta tccaaagaca
     1201 atggaagagt tgggacaaaa tacaggttat ctactttacc gaacaatgat agagagggat
     1261 gcagaggaag aacgattacg ggtaattgat ggcagagatc gcagccaatt atttttgaat
     1321 caagaattac aagctaccca atatcaaaca gaaataggtg aagatataca agttagtatg
     1381 acagaagaaa tcaatcaagt ggatattttg attgaaaata tgggacgtgt caattatgga
     1441 cataaactct ttgcggaaac gcaaaaaaaa ggaattagaa cgggagtaat ggcagactta
     1501 cattttatta caaattggca acagtattca ttgcctttgg aaaattgtaa aactgtggat
     1561 tattcaaaag gatggcagcc ccaacaccca agtttttatc gatatgagtt tcttttagag
     1621 gatatcggtg attgttttat tgatttaagg aaatttggta aaggaattgt ctttgttaat
     1681 cagaccaata taggtcgttt ttgggaagta ggtccaacgc tttcactgta catctctagc
     1741 ggctttctaa aagttggtaa gaatgaaatt gttatttttg agacagaagg tacctatgaa
     1801 ccagaaatca atttattaaa agcaccatta tataaagaaa tgaaagaggg atgaacaatg
     1861 agtattattg gagtaagaat tgatggacgt ttaattcacg gacaggtagc caatttatgg
     1921 acaaccaaat tgaatatttc tcgaattatg gttgtggatg atgaagtggc tcaaaatgat
     1981 attgaaaaaa gcggattgaa attggctacc ccagcagggg tgaaactaag tattttgccg
     2041 attgaaaaag cggcgcaaaa cattttagca ggaaaatatg attcacaacg tttactgatt
     2101 attgcacgaa aacctgatcg attattaaaa ttggtagaat tgggcgttcc aataaaagag
     2161 attaatgttg gaaatatgtc acaaacttct gagactcgat caattacgaa atcaattaat
     2221 gttgtcgatc aagatgtaga aacgttcaat caattaaatg aaaaaggtgt ccatttaatt
     2281 gcgcaaatgg ttccaagtga caaagcagat gactttatgt ctctgttaaa taaataataa
     2341 tatacgagga ggtgaaaaga tggcggggca tttagtttta attagtcatg gacgattttg
     2401 tgaagagtta aagagaagtg ttgatatgat catgggagta caaaaagata tttatactgt
     2461 agggttatta cctgaagagt cagcaaaaga ttttcgagta aatttcgaaa aaacaattaa
     2521 tggtttgaaa aaagttacag ttctggctga tctaatggga ggcactcctt gcaatgttgt
     2581 ttctcaatta attttagaag gaaaagaaat agaactatat gcaggaatga atatgccaat
     2641 ggttataggc tttgttaata atcaattttt aggagaagat agttctttgg taaataaagg
     2701 aagagaaaat attatacatg taaatgaata tttggaagta aatccaatag atttagaaga
     2761 tgaatgaatg tggaggaatt tatg
//
LOCUS       DAZKCZ010000167         2702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000167 DAZKCZ010000000
VERSION     DAZKCZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.171"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..1101
                     /locus_tag="ACG8KN_002512"
     CDS             316..1101
                     /locus_tag="ACG8KN_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9189510.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1114..1965
                     /locus_tag="ACG8KN_002513"
     CDS             1114..1965
                     /locus_tag="ACG8KN_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9189511.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2089..2541)
                     /locus_tag="ACG8KN_002514"
     CDS             complement(2089..2541)
                     /locus_tag="ACG8KN_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189512.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
BASE COUNT          854 a          453 c          557 g          838 t
ORIGIN      
        1 gctgtgacaa aagtcttgtc acagcctttt tttccaaata aatggttttt cagaagtagc
       61 ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gtaatttttg taaattactt
      121 cttatttctt gaagctggcc acttctgtca caacctcttt ctttatagaa ataaaaggac
      181 actagcaatt catgaaaacg attgctagag cgtatcattt gctttagact atatatcttg
      241 gtgtacactc actttaagac ggaaataaaa gttatccgta ataacaggtt taggtatgaa
      301 aaaggagtgg gtactatgta taaggaatta gaaaacaaag tcgcagtagt aactggtggc
      361 tcaaagggga ttggaacagc gattgctcaa cgattcggga aagaaaaaat gaatgtagtt
      421 gtcaactata actcggataa agaaggagca gaaaaagcag tcaaagcaat cgaagaggca
      481 ggcggaaaag cagtctctgt acaagcagat gtcagttctg aagaaggaat acagcgttta
      541 cttgatacgg ctttagaaaa ttttgggaca ctggacgttt ggattaataa tgctgggatg
      601 gaaaatcagc atccaaccca tgaacttagc cttgaagatt gggaaagagt attaaaagtc
      661 aatttgactg gcgtttttat cggaacgaaa gcggctttaa attactttac agagaatgat
      721 aaaaaaggaa atatcatcaa tatgtcttct gttcatgaac aaatcccatg gccaacgttt
      781 gctcattacg ctgcttcaaa aggcggagta aaactattca ccgaaacggt agcaatggag
      841 tacgcaccaa agggaatcag aatcaatagt ataggacctg gtgctatccg tacacctatt
      901 aatgcggcaa aatttgatga tcctgaacaa aaagaactct tggaaagtat gatcccacta
      961 ggacgcgtag gtgaaccaaa agaaatagcg gcgacagctg catggttggc ttcagacgaa
     1021 tccagttatg ttacaggtat tacactattt gcagatggtg ggatgacttt atatccttct
     1081 ttccaaggtg gaaaaggata agggtgaata aaaatgggtt tattgattgc attgatcccg
     1141 gcagttgggt gggggatcca gccactgatt ttgaaaaaga ttggtggcag accaacaaat
     1201 gagatattag gaactggtat tggagccgtg ctcgttggct tacttgttca attattcgct
     1261 tctccagctg gtatttcagt gactacattt ttgattagtt tactttctgg tgcattctgg
     1321 gtcatcggac aaacaggaca gtatacatct ttcaacttga ttggtgtttc taaaacgatg
     1381 ccaatttcga ctgcattaca gttagtcgga acctcattga ttggtgtctt cgcttttgga
     1441 gaatggtctg gaagtatggg aaaaatcatt ggtgcagcag caatcattct tcttgtattt
     1501 ggttctgctt taacggcaat cagtgatgat cctggaaaaa aaggcggtat cacaaaagga
     1561 atcagtctat tagcatcaac aagtattggt tattgggttt acagtgcatt gcccaaacta
     1621 gttgatgcaa gcggactttc cattttcttt ccgcaaatga tcggtgtctt tttaggggca
     1681 gtgatttatg tgattttccg aaaaccaacc gcattttctg aagacaagag ttggaaagca
     1741 acaatagttg gagccatctt cagtgtgtca gcattggctt atattttttc agcaaatgaa
     1801 aatggggttg ctactgcata tatcattaca cagctaaacg tagttatctc tacattaggc
     1861 ggtatgtaca tcctgcatga aaataagaca ccaaaagaaa aaaaattaac gattatcgga
     1921 ttattgctga tcgtagtagg aagcatggtc acggtcttta tttgaggata aagatggtaa
     1981 aatctcattt attaaaaaaa tatttgtttg aaccaaacag taagtactta taaaaaaata
     2041 gcgctagaat ttccaacatc tctggaattt ctagcgcttt ttaattgttt aagatacaag
     2101 tgaaatatct gtctgcatca ataatttttc attgatccat tgactaaagt ttacgacata
     2161 attgaagtta ccaccgatta gtatctccgc atgattgtct ttataaagat cgtattgtaa
     2221 aagacggtct tttttatttg cgtaaacatc gttatggtac tggactaatt gttctcggaa
     2281 ttgattgatt gctgtaaaat cacgatactg aaggttgaaa cgcatcgtga ttggtgcatt
     2341 tagcatgaat cgctgcattt cttttgctgc ttcctctgct tgatctccgt ataggaactc
     2401 aaaaagatta cgttcagaaa atttccattg cccgccaacc tttgttcctt ttagttttcc
     2461 ttcatgaaga taatttctga tagaacgtgt agtaacacct aattgttcgg ctaattcatt
     2521 tactgagtaa aaatctttca atgttcaccc tcctatgtga atggttctac tgacttagtt
     2581 gatatttgct taatcacgaa atgtagaaag aacgatttta attaaggtca ttgtaaaaat
     2641 caagtcagaa aattctaact tttctttctc taaagaaatt cgttctctcc taaagtgata
     2701 tt
//
LOCUS       DAZKCZ010000168         2653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000168 DAZKCZ010000000
VERSION     DAZKCZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.172"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..352
                     /locus_tag="ACG8KN_002515"
     CDS             <1..352
                     /locus_tag="ACG8KN_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HFP9189513.1"
                     /translation="QIERIREANEIPYIYHKSYLPEQYINSNYPEINYYNSIYKRLKS
                     DYNIDLNEENFRETNEILFPAPNSVCKQLKINESEPVVFQTKLSCLSENNKPIEYVES
                     YKKWNFYKIQLLSN"
     gene            433..1545
                     /locus_tag="ACG8KN_002516"
     CDS             433..1545
                     /locus_tag="ACG8KN_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HFP9189514.1"
                     /translation="MKHISVLIKPASSLCNLRCSYCFYANVSSLREVRSFGKMKEEVT
                     EKMIENIYADLEDGDQLTLAFQGGEPTMAGLNYFRTVIRLVAQQQKQVQVHYAIQTNG
                     TMINEKWCAFLKEHDFLVGLSIDGHPLYHDLHRVDPKGRGTFQRVLQTKQLFDYYEID
                     YNVLCVLTNPLAKEAKKVFRFLKEQQIDFVQFIPCLDDLDAKKRNSYALTPKRFAGFY
                     HQLLQLWLQELAEGHYISIKLFDDLLNLLIKRQVTACGILGNCQVQYVIEADGSVYPC
                     DFYVLDEYRMGYIQEQTLRQLFEQDIAKTFLCGRPEMPKKCLSCPFQKMCRGGCKRMK
                     DAIYVDNTGYCGYQQLLQEFVPQINRILGLLQGVSV"
     gene            1542..2318
                     /locus_tag="ACG8KN_002517"
     CDS             1542..2318
                     /locus_tag="ACG8KN_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9189515.1"
                     /translation="MIGFIYFIVIVLANTVGAVSGMGGGVLIKPIFDFIGAHSVAAIS
                     FYSTVAVFTMSLVSTARQLASGRKIHWQIVLWVSGGAVLGGILGNVAFDHLLLLFQNE
                     DEVQMIQIFLTVVTLLFAFFYTKYDWPGFQLKNNFWYCFCGLVLGFLASLLGIGGGPI
                     NVSLLMLMFALPIKEATVYSICTIFFSQLAKIVTIAGTTGFGLYDLKILWFVIPAAIV
                     GGLLGAKVSTVLSPQKVTLVFQTVIFLVLIINLYNGYQLL"
     gene            complement(2383..2513)
                     /locus_tag="ACG8KN_002518"
                     /pseudo
     CDS             complement(2383..2513)
                     /locus_tag="ACG8KN_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031600262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          775 a          486 c          538 g          854 t
ORIGIN      
        1 tcaaatcgaa cgaattagag aagctaatga aataccttat atctatcata aatcttattt
       61 gccagaacaa tatataaatt ctaattatcc agaaattaat tattacaatt ctatttacaa
      121 acgtttaaaa tcagactaca acattgattt aaacgaagaa aactttaggg aaacaaacga
      181 aattttattt cctgctccta atagtgtatg taaacaacta aaaataaatg aatctgagcc
      241 tgtcgttttt caaaccaaat tatcctgctt atctgaaaat aataaaccta tagaatatgt
      301 tgaaagttat aaaaaatgga atttctataa gattcaactc ctatctaatt aactactact
      361 gaatttgatt ttactttgtt cgttatagca atttttcata attaaactat gttaaaaaat
      421 gaggttctta ctatgaaaca tatttcagta ttgatcaaac ccgcatcttc cctctgcaat
      481 cttcgctgca gctactgctt ttatgccaat gtcagttcct tgcgagaagt ccgctcgttt
      541 ggcaaaatga aagaagaagt gacagagaaa atgatcgaaa atatttatgc agacttagaa
      601 gacggcgatc aattaacatt agcgtttcaa ggtggagagc caacgatggc gggcctcaat
      661 tattttcgta cagtgatccg gttagtagcg caacaacaaa agcaggtaca ggtccattat
      721 gcgattcaaa cgaatgggac catgatcaat gaaaaatggt gtgctttttt gaaagaacat
      781 gattttttgg tcggtctctc gatcgatggc catccgctct accatgatct gcatcgggtc
      841 gatccgaaag gaagaggaac ttttcagcga gtgctgcaaa caaaacagct gtttgactac
      901 tatgagatcg actacaatgt actttgtgtc ttgaccaatc cgttggcaaa agaggctaag
      961 aaagtctttc gctttctaaa agagcaacaa attgattttg tccagtttat tccttgttta
     1021 gatgatctag atgcaaaaaa aagaaatagt tatgctctca caccaaaacg ttttgcagga
     1081 ttttaccacc agcttttgca gttatggctg caagaattag cggaggggca ttatataagt
     1141 atcaagcttt ttgatgatct tttgaatcta ttgatcaagc gacaagtcac tgcctgcggg
     1201 attttaggaa actgtcaggt acaatatgtc attgaagcag atggcagtgt gtatccttgt
     1261 gatttttatg tgctggatga gtatcgcatg gggtatattc aagaacagac tttacgtcag
     1321 ttatttgaac aggacattgc aaaaacgttt ttgtgtggac gccccgaaat gccaaagaaa
     1381 tgtctgagtt gcccgtttca aaaaatgtgt cgtggcggct gcaaacggat gaaagatgcc
     1441 atctatgtag ataacacagg ctattgtggt tatcagcaac ttttgcagga atttgtaccg
     1501 cagattaatc ggattttagg tttgttacag ggggtaagtg tgtgattggg ttcatttatt
     1561 ttatagtgat cgtgttggcg aatacggttg gtgccgtttc aggcatgggg ggcggtgttc
     1621 tgatcaaacc catttttgat tttatcggtg cccattctgt tgcagccatc tcgttttatt
     1681 ctaccgtagc cgtgtttacc atgtcgctgg tttcgaccgc aagacagttg gctagtggac
     1741 gtaaaatcca ttggcaaatc gtcctttggg tttcaggcgg cgccgtctta ggaggcatct
     1801 tgggcaatgt cgcttttgat cacctgctgc ttttatttca gaatgaagat gaggtacaaa
     1861 tgattcagat ctttttgacc gttgtgacac tgctgtttgc tttcttttat accaaatacg
     1921 actggcccgg attccaactg aaaaataact tctggtattg cttctgtggc ctagtccttg
     1981 gttttctagc gagtctgctg ggcattggcg gtggaccgat caatgtgtcc ttgctgatgc
     2041 tgatgtttgc tttacccatc aaagaagcca cggtctattc gatttgtacg atttttttct
     2101 ctcaattggc gaaaatcgtg acgattgctg ggaccactgg atttggcctc tatgacttaa
     2161 agattttgtg gtttgtgatt ccagccgcga ttgttggcgg tttgttaggg gcaaaagtca
     2221 gcactgtcct ctcaccacaa aaagtgacgc tggtcttcca gacagtcatc ttcttggtgc
     2281 taatcatcaa tctttataac ggctatcaat tactataaaa aacgagtatt atcaaccact
     2341 tatagaaaac gattaattag taactttaga agtactttct cattaagaag cgctcttttt
     2401 tgtgactttt ctgaaactag aataaatact ctcaattgca ttagtaatat gcatgatttt
     2461 tctaatagca ctgcctgttc accatgtgca aagtaccgtt tccagacatc tactaacaaa
     2521 ataggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctgg gtcagaatga
     2581 accacattct ggctcagatt ttttgttatc ttacattgat taattgattg atcaaaaaga
     2641 ttctaattct cat
//
LOCUS       DAZKCZ010000169         2590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000169 DAZKCZ010000000
VERSION     DAZKCZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.173"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..668)
                     /locus_tag="ACG8KN_002519"
     CDS             complement(15..668)
                     /locus_tag="ACG8KN_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9189516.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            956..2230
                     /locus_tag="ACG8KN_002520"
     CDS             956..2230
                     /locus_tag="ACG8KN_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9189517.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            2285..>2590
                     /locus_tag="ACG8KN_002521"
     CDS             2285..>2590
                     /locus_tag="ACG8KN_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681079.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFP9189518.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     "
BASE COUNT          810 a          508 c          420 g          852 t
ORIGIN      
        1 tatctttaat tttttcattt tctctccaat ttatatccta catttcttat agtagttata
       61 cagtctaatt gtaatttttt tctaagttct tttatatata catctattac tcgatcataa
      121 ggagtttctt cgctatcttt ccatacataa tcaataattt gcattcttgt taacacttgt
      181 ccattattat ccaataaaca ttttaatact tctagttctt ttgcatttac atctatttct
      241 tcaccattta ttttagctgt atagctatta aaattaaccg atagatcctt atattcaaac
      301 ttctctaaat gtccataatt cttcttaatt aaagaatcta ttctagcttt taagaccggc
      361 aatgaaaatg gcttttctac atatccgtct actaaattag taaatgcatc aattttatat
      421 tcttcatcac taaatgcagt caagattaga attggtaaat tgcttttctt tctaatttct
      481 ttcaacactt ctaaaccatt tataaaaggt atctgaatgt ctaagataac caaatttata
      541 tcgctattaa attttgacaa ggcttctctt ccatctttag cttgaataac agtatatcca
      601 aattctgaaa gatattcact tattccctct cttatcatat tatcatcttc aacagttaat
      661 attttcatat atacctccac ttaagcctat acactagata ttatacagca tattatggct
      721 ctataatatc tagtgttggt gacaccttct gttgccaata ctttttcttt tttctaataa
      781 atgcaaacag acaccttgaa atcctttaga ataaagggtt ctataatatt ttgtgttgtt
      841 ggaaaatcga aggcgatttt ctactaacac tttttcttcg tttcaactca aaaagagact
      901 cacttttggt aaaatttagt cgaccaaaac atcaccctac caaaggagtc gctctatgga
      961 taatcatact agaaaattac tcggattaac agataaacat ttattttttg atgaagaatg
     1021 gctgattgaa aaagaatata aaggcgttca agcgcagttt attaaaggga gattggatga
     1081 ctcaccttcc cattgtgaac actgtggatc gattcgtatt atccgcaacg gttcctatac
     1141 gacacgtact cagatattga aagtcaaaga aaagttgact attttagaat taaaacgtac
     1201 ccgttttcta tgctatgatt gtgggcagac cttttctgcg aaaacggact tagttgatga
     1261 acatcatcaa ctaacaaagg aactgaagca ggcgattctc atagaactgt acgaaaatca
     1321 atcacgtaaa ctaatcgcta agaagtactt cgtctcggat gggacggtga cacggatctt
     1381 gcgtgaagca acgaagcatt accagccacg tatgaacttc cttccgacgg tgttgtgtat
     1441 ggatgagttc aaatcaatga agtctgtttc tggttccatg agttttattt gtgttgatgg
     1501 gacaacccat cagctattta cgatccttga agatcgcaga ctttacaaac taactcaata
     1561 cttcctgcgc tttcctagaa aagcccgttt aaaggtcaag tacctagtga tggacatgaa
     1621 tgccagctat tgtcagttgc ttaaaacagt ctttccaaat gcggagatcg taactgaccg
     1681 ctttcatatt gtccaacaca tcaatcgcag cttcaatcaa ttacgtgtcc agatcatgaa
     1741 tcgttttcga acctcacact ctgaaaatca gaagaagtac cgtcggctca agcgatattg
     1801 gaaactctta ctcaaagatt ccacgacatt ggagccactc aaacggcatt atcatcgact
     1861 gtttaaacga ccaattagtc aaacagaaat cgttgatgag ctgttaagct acaacgaaga
     1921 attgcgaact gcgtatcatt tttgtcaatt attacgctat tatttcgtta aacgaggtac
     1981 ggaaggcttt caagaaaccc ttaaaacaat ttcatctacg ttacctcagt cgtttaaaaa
     2041 gaaattcacg atcttccatc gctaccaatc gggtatttca aatgccttta aggtgtccca
     2101 ctcaaacggc gtcactgagg ggctgaataa caagatcaag ctgattaaac gaattgcgtt
     2161 tggttatcgc aacttttata actttcgggc acggatctat ttacaacaag gtctgatctt
     2221 tgaaaactaa aaaaatatgc ggcgcacaga acgcgccgca tatcggacta agttttattc
     2281 aaatttgtct tcaccaacac gatttgacga agagccaaaa attatcgtac ttgattacgg
     2341 tagtcagtat aaccaactga tcactcgccg tattcgtgag ttcggtgttt tctcagagtt
     2401 gatgagccac cgcattaccg cagaagaagt aaaggcaatg aaccctaaag gaatcatcct
     2461 ttctggcgga ccaaacagtg tttatgatga gaaggccttt ggtattgatg aaaagatttt
     2521 tgatttgggg atcccagttt taggaatctg ttacggtatg cagttgatta cttataaatt
     2581 aggcggaaaa
//
LOCUS       DAZKCZ010000170         2518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000170 DAZKCZ010000000
VERSION     DAZKCZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.174"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1087
                     /gene="yidC"
                     /locus_tag="ACG8KN_002522"
     CDS             158..1087
                     /gene="yidC"
                     /locus_tag="ACG8KN_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9189519.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            1208..2518
                     /locus_tag="ACG8KN_002523"
                     /pseudo
     CDS             1208..2518
                     /locus_tag="ACG8KN_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320902.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          869 a          447 c          483 g          719 t
ORIGIN      
        1 ccaagtattt ctttcttgat tttttgtctt ttttcaaaat ttttcgctac gaaaaagaaa
       61 gaatatttat atgaaatttc tatgaagttg attgaattcc atgcgctttt ttagtatagt
      121 taagtttgtg taaaaattat agaagaggaa gtattctatg aataaatgga aaaactggtt
      181 attgggatcc ggacttgtca gtatcctgct ctttttatct ggctgtgtgc ggatggattc
      241 aaacggaaac cctgacactt caggaatcgt ttatcgagta ctcgtccatc ctatgggcca
      301 agcgattaca tatctagttg agaatttcaa ttggtcttac ggctgggcag ttattgcaat
      361 gactgtgatc gtccgtatca tcatcttgcc actaggtatc agtcaatcaa aaaaaacaat
      421 gatccaatct gaaaaaatgc aagcgttaaa accacaagtt gaagcagcac aacaaaaact
      481 aaaagctgca acaacacgtg aagaacaaat ggcagcacaa gcagaaatgc aacaagtata
      541 tcgcgaaaac ggattgagta tgaccggcgg aatcggctgt ctgcctctat tgatccaaat
      601 gccgatcttc tctgccttgt attttacggc tcgctacaca gaaggtatcc gtgaatcatc
      661 tttttacggc atcgatctag gctcaccaag tatggtgctt gttgccattg caggggtcgc
      721 ttatctgctt caaggatata tttcaacaat cggtattcct gaagaacaga aaaagacgat
      781 gcgtacgatg ctgatcgtct ctcctgctat gatcgttttc atgtcgatca gtgcgccagc
      841 cggtgttaca ctttactggg tagtgggtgg tgtgttcagt tgtctgcaaa cattcatcac
      901 aaacgttatg atgaaaccac gtttgaaagc acaagtggca gaagagttga aacaaaatcc
      961 gccaaaacaa gtcgtcactc caagaaaaga cgtgacagaa tcggcaaagc cagctgacaa
     1021 taaacaacca aaaaacagta caaacaacaa aggccgcaac gcaggaaagc agcaaaagcg
     1081 gaaataaaaa tatatgacaa aaaactgtag acaaataaaa aaatttcttt gttctacagt
     1141 ttttttgtta caatcaaaga aaacgtcgct tttcgaggag gaatgtataa tgatgtccaa
     1201 ataatcaatg gatagtcgaa tggaagaagc caaaatttcg ctagatgcac ttgttcctaa
     1261 aaatcatttg gtccataaaa ttgacaaaat attgttattt gattttattt atccaattgt
     1321 tgaatcaact tactcgacca ttggtcgtcc tagcgttgat cctgtcatct tagtaaaatt
     1381 agtattcatt caatatttat ttaggattcg ttcgatgcgt cagtccatta aagaagctga
     1441 aacaaaccta gcttatcgtt ggtttcttgg gcttagttta atcgataaaa ttcctcattt
     1501 ttcaacgttt ggtaaaaact atgtacggca tttcagagaa accgattgtt tgaacagata
     1561 tttatcatta tccttgaaca agcagttgaa gctagattta ttcatgaaga aattttgtat
     1621 atggattcaa cctatattaa agcgaatgcg aaaaaacata tttttacaag agaaatggta
     1681 cataaataag ccattttata ccaaaaacaa ttgaaagaaa aaattaatga agcaagaatt
     1741 gcagaaggca aacgtccttt tggttcaatc attcatcagg aggtggtaga agggaaaatt
     1801 agtgtcgcag atccagaaag tggttattat gtaaaaacag aacaagaaaa acagtttgct
     1861 tattctgctc ataccgtttg tgatgaaaat ggttttgtat tagatgtcat gattacgcct
     1921 ggaaatctac atgacagccg aatgctcgtg ccacaaatcg aacgtgttaa atcatgttgt
     1981 ggtgtagctg ctgatgctgc gtacaaaaca ccttggaacg ccaagtattt aatcgatcga
     2041 aaactacgac ccatttttcc ttatactcga cccaaaagaa gcaaagaacg ttttaaaaag
     2101 aaagattttt attatgatcc ctattataac tggtacctat gcccaaatga tcaaacactt
     2161 cagtttcgca caactactcg agatggttat aaaaaatatg tatctaaatc ttttatttgt
     2221 gaaagttgtc tattattgaa aaattataca gaaagccaga aacaccagaa agaaatacat
     2281 cgacatattt aggaagacta cttagatgaa gcagaacatt tacgccatac ggtgtacaat
     2341 cgcgaaaaat ataaaaaaaa gaaaagaaac gattgaacgc gtttttacag acgcaaagga
     2401 aaagcatgga atgcgctaca ccaaatatcg aggcttggaa aaaaccaggt gcatacgatg
     2461 cttgtttttg ccgcaatgaa tctgcaaaaa ttagccattt ggcagtgggg gcatatgt
//
LOCUS       DAZKCZ010000171         2484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000171 DAZKCZ010000000
VERSION     DAZKCZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.175"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..513)
                     /locus_tag="ACG8KN_002524"
     CDS             complement(148..513)
                     /locus_tag="ACG8KN_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189520.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            721..1203
                     /locus_tag="ACG8KN_002525"
     CDS             721..1203
                     /locus_tag="ACG8KN_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189521.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1400..2290
                     /locus_tag="ACG8KN_002526"
     CDS             1400..2290
                     /locus_tag="ACG8KN_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFP9189522.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          862 a          401 c          495 g          726 t
ORIGIN      
        1 gcaattatta cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa
       61 aactagtgat agttttggtg atataagaaa aacatatatg tataaaaaga agaaagtagc
      121 ttctctttgt acatatatgg tttgttttta tggatgccga gcttttttat aagataaggc
      181 taccgcccat aacgacccaa ggatttgtgg aactaagtaa atacctgcaa gaagcaagat
      241 cagctgggtc ggttttaaag gatgatagaa gtaacgccat tcagtaaaat tggcgcaaaa
      301 taaataggaa gtgacagtag atataatatt ggcaatgaat aaagatataa tacgcctgtt
      361 cctttgaaaa taatatccag caaaaagaga taaaaatatc aatgtgatga acccgcttac
      421 ggaatgataa cgaaaatcaa gcagtaaggc gaaatgcaca taaggaacaa aagataaaag
      481 caacggaagg tttttccagc aagtttttat catctttcat caccacctaa caagtgttat
      541 tattttgtta gttttattgt aaattgaatc ctgcaataat caattacttt ttcattattt
      601 ctagattaaa tgaataaact ctcatgccat tatctctaaa aaaaaagttt ttccccctaa
      661 tttttttaga tagaaaattg tatagtatga tgagagaaaa agtagtttgg gggaacgtta
      721 atgagaagaa aagcagtaat cgatcggctt tatttatttt tttggttttt tcctatcgtt
      781 ttgctacctt ttacatgggt aaatgctgtt gtacaaggtg cggagatatt aggcctgatc
      841 caaggaagcg gatttgtggt ttctaggaat tttccagctt tgattgtttt ctatttagtg
      901 ataagtgtag gtctcttatt accaaaatct ctgatcaatc actacaattg gcagctgctt
      961 tacttttctt gcttattagt atttacatct atgttttctt tgctgcctcg cttgattatc
     1021 ggccctaatg gtttgagcga gagtggattg aaggatttag cttattcctc catgtatttc
     1081 ttgacgatca aaccatggtt ctatttagta attgcctcat ttattcttgc aacatttttt
     1141 tacttctgga cagaaatcag gataaaaagt gaacgatctg tatctataaa aaataactat
     1201 tgatggtgga tcatcagtaa aaaaacatat gagctgcgta aaatgcatgt ttctcatatg
     1261 ctttttttat tattatgtaa agaatgcgaa aaacgtttac atgattttta cataacttaa
     1321 ctatattttt acagcaacca tacgagggat ttacagtcga ctgataaact ttagttgtaa
     1381 ataagaaagg agcaataaaa tgaaaaaagt attgtttact ctaggtatga tgggaattct
     1441 tttagccgga tgcggctcag gatcaggaaa tacagatggc tctgccacac aaaataattc
     1501 aagtgactca aatgaacaag taaaaatcgt tgcggtaggt tcgacagcat tacagccgtt
     1561 agtcgatgca gcacaagaaa gctttgtaca agaaaatcca aattatcaaa tctctgtaca
     1621 aggaggagga agcggaactg gtttgagcca agtcgaagct ggagcagtta ctatcggaaa
     1681 ctctgatgta tttgcagaag aaagagacgg agttgatgct tcaaaattag tggatcataa
     1741 agtagcagtg gtcgggatgg caccaatcgt caataaagat acagatgtca aagatatcac
     1801 caaacaggaa ttgattgata tctttacagg aaaaatcact aactggaagg aagttggcgg
     1861 gaaagatcaa aaaatcaacg tcgtgaaccg tgcaaacggc agcggtaccc gcgcgacatt
     1921 cgaaaaatgg ggactggatg gtgctacccc tgtgcagtcc caagaacaag attcatcagg
     1981 aacagttcgg cagctggtaa gtcaaacacc aggagcaatc agctatttag ctttctcata
     2041 tctcgatgat tccacacaag cactaagtat cgacggtgta gaaccaaaag aagagaatgt
     2101 agcagataac agctggggaa tctggtcata tgaacatatg tacacgaacg gaaaaccttc
     2161 ccctgaagtc caaaaattct tagactacat gatgacagaa gaaatccaag aaggacctgt
     2221 caaagaattg ggctatttac caatcacaat gatggaagta gaacgtgatc acgaaggaaa
     2281 tatcaaataa ctagcagata aacatgccac ggtagtcgtt actattaagg aaaaatcaac
     2341 tatgtttccc cacatataca tttttccttt aattgtgaaa aatagttcct catattttgc
     2401 cgttttttga gctaataccg agggtcagct gattgaacac cgtttattcg aaaaaaagag
     2461 ctacgacata gaactgtcgt aact
//
LOCUS       DAZKCZ010000172         2465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000172 DAZKCZ010000000
VERSION     DAZKCZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.176"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..185
                     /locus_tag="ACG8KN_002527"
     CDS             <1..185
                     /locus_tag="ACG8KN_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189523.1"
                     /translation="SKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVPS
                     LFFIVFFAVGYFIIKI"
     gene            690..884
                     /locus_tag="ACG8KN_002528"
     CDS             690..884
                     /locus_tag="ACG8KN_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189524.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1075..1324
                     /locus_tag="ACG8KN_002529"
                     /pseudo
     CDS             1075..1324
                     /locus_tag="ACG8KN_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1568..2398)
                     /locus_tag="ACG8KN_002530"
     CDS             complement(1568..2398)
                     /locus_tag="ACG8KN_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFP9189525.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
BASE COUNT          735 a          436 c          487 g          807 t
ORIGIN      
        1 tgagtaaaaa ttctaaagga ttcctaacca tcttattagc ttttataggg tatatgttgg
       61 ttgggctgct aaaatcatac agtaatgagt tgctgaattt ttcaacattt attaatgata
      121 cattagttcc aagtttgttt ttcattgtat tttttgctgt tggttatttt ataataaaga
      181 tatgatgact aatttagctc aactattcca gcgtcagctg aagatattgc aattcccgat
      241 tccttaatca tttatctgga tgagacagaa agtgaagttg caacagatgt gcatgtagtt
      301 gaggataaaa atgatagttt aaaaattaat agttcagata ctttactaag agtaagtcct
      361 atcttttcag ctgttattta gatgcaagta gcaactaagt taggtagtac tcctgcaatg
      421 gataggtatt tgtgtgttcg atatataagt ggtcgaagta tcaaggttat gtatactgga
      481 tgggagatag aagagttaaa aattggaggt caccgccaca aatggaattt agatttagag
      541 gtaccttaga tttggtatag ttattaaaca caagatggcc tggtttgtag ggaaagtcga
      601 agagtatttt tccacttctg cgccccttta atttttctag taatgccgtg aatttgtgat
      661 taattggaaa tatgaagaaa ggagagtcta tgaaaacaac actagttcta ttctataaaa
      721 agcatcctta ttttacactt ctgataaata tattgttggc tagtgttatc gggatttctg
      781 ttgaatatct aataaataaa gatttcatag gttcttgttt ttatacggct ttatttcttg
      841 gacttcttga agcattttcc atttataaaa aatcaaaaaa ataaaataga ttgccgtaag
      901 agatcttttt catttcattt ttttctgaaa acccgttgtt tctgtcagtg aagcagcggg
      961 tttttagatt tattgtcttt agacctaatt gagcgcgccg agcgattgaa atacaaaacc
     1021 acttgctatg cgggtggagc aataaaggga aatagtttag cccgtacaaa gtagatgtgt
     1081 aaataccaca ttgtattcac accaaagtat agaagaaaaa taatttataa tcaatatcga
     1141 gccaatattc aggagatcat caagagattg tgcaaatcta aaatataaat ttgggtatcg
     1201 acacttttgg gtggaaggat actatgtgac aaaagtagga caaaatgaag cgaccatcaa
     1261 aaagtacatt caagaacaag aaaaacatga tagtgcgtta gataagctga gtgtacgaga
     1321 gtaagacgat ccttttaagg gctaaggagt agtacataaa ccttttaaga ggtgggcaaa
     1381 actggcaaag gcaatatggc ttgaacataa aatatgaaag ccagcgtctt taggcgctgg
     1441 ccggtgattt gagcttatag ctctagagaa aaccaccctt ttgaagggtg gttatgatta
     1501 atgactttag tcagttttgc tcacaaagcg tgtggaacaa aaaacgggtg tttctacggg
     1561 tagatattta tttcttgaat ggaggtgtcc catacatttt tggtgttact ggttctaatt
     1621 ttggtgcagc gccttcgatc ttaggttctt tcaaccattg atattcgcct tcgccatcca
     1681 gtgctttgcc actagcccaa gaaccttcac tactttggtc tccctctgag aagttataga
     1741 agtcataagc aatgtctttt cgttctttgt cacgcgggaa agtacttggg actaagattg
     1801 aaccttcttt ttcttctaat tctttcactg cacattccca tagattttga tgatagctat
     1861 ctcttgcaag gagaacagac agcatatctt ttacgccacg atcatcgatc atttcgtaaa
     1921 tacgtgctac ttgaagtctt ccttgtgatt cggcatttaa attagctcta aagtctgcta
     1981 acaagttgcc actcgcaatg atataggcac cgctccatgg attgcccacg ctgtcagcag
     2041 gacgaggtcc taagccagct acgatcgcgt gctgtggatt cattccagag ataatggcac
     2101 cgattgttgg atctttttgc atcgctgctt cttgatcttc aacaggcgca ccctctaata
     2161 aacgagcaac cattgtcgcc aacatttcca cgtggccgat ttcttctgtt gcaatgtcca
     2221 tcagcatatc tttatatttt tcttctcctc ggcagctcca gccttggaac aggtactgca
     2281 tagctacact catctcgcca tattggccgc cgatcagctc ctgcagatat ttcgcgatta
     2341 atggattcgg acgatctggt tttgcttcaa actgcaattc tttctggtgt ctaaacatca
     2401 aaattcctcc taatctattt ttaatacatt ctcattctgc tacgaaagat taaaaaattc
     2461 aaaga
//
LOCUS       DAZKCZ010000173         2421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000173 DAZKCZ010000000
VERSION     DAZKCZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.177"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..2274
                     /locus_tag="ACG8KN_002531"
     CDS             121..2274
                     /locus_tag="ACG8KN_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFP9189526.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          827 a          425 c          536 g          633 t
ORIGIN      
        1 aaaagttgat tttatcttga aattaggtaa agaatttctt gctccgccca ttttttcttt
       61 tttattaaga agtttttggg tacaatgaag catgaatgta aataggcgga aggataggaa
      121 atgatgaaag attttatgaa aaaaatatta aatacttctt ggttagaaaa aataagaaaa
      181 ggtgcacaag aacctaagaa caaaacgttt cgccggtccc atgttccttt tcgcttaaat
      241 tttctttttt tcattatatt tacgttattt gttgcgttga ttgctagatt gggttatttg
      301 cagatcgtga acggcgaaga gatggaggcg cgagtgaagt ctacttctac gattacggtc
      361 caagaaagta ctcctcgcgg aatgatctac gactccacag gaaaagcttt agttacgaat
      421 aaagcgaatc aggccatcac atatacgaga ggaacacaag ttacggctaa agatttgctt
      481 gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg tagatgaaaa tctgacagat
      541 agagataaaa aagatttttg gctggctgat cccaaacatt tgaaagaagc cacaaaaaga
      601 ctttcggcaa aagaaaagca attagggaac agcgaacaat atagtgcgat ggtcaataaa
      661 gtcacagctg atgagattaa tttaaacgag gagcaattaa aaattgctac catctttaaa
      721 cggatgaata gtgcatatga attaaacact gtctatatca aaaactctgg tgttactgat
      781 caagagttag ctgttgtggc agaacgcgct tctgaacttc caggagtttc gactggcaca
      841 gactggacac gagaatataa tgcagcaagt tcactgaaaa gtattcttgg cagtgtgacg
      901 actgaaaagc aaggacttcc agcagatgaa gcagagaaat acctagcaaa aggttattct
      961 cggaatgacc gtgtaggaca aagttatttg gaaaaacaat atgaagatgt cctgcaagga
     1021 accaaaaccc aatatgaagt ttcccttgat aatgaaggaa atgtttcgaa tcaaaaagaa
     1081 atcttctctg gggaaaaagg ttctaattta atgttatcta tgaatgcaga attccaatca
     1141 aaagtagaag aaattttgaa aaggaattat caaacattga tcaacaacgg caaagcacag
     1201 tattcgcctg gtgcttatgc agtggctatg aatcctcaga caggagaagt gctagcaatg
     1261 actggtttca gtcatgaaca aggttccaaa gaaatcaccg agaatgcttt aggcacgatt
     1321 actagcgcct ttgatccagg gtctgtagtc aaggctggta ctttgacagc aggctgggca
     1381 agtaacgcga tcagcggaaa tcaagttttg attgatgagc cgattcgtct gcaaggtgct
     1441 tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg ctcttgatgc agtcaaagca
     1501 tcggaattgt cttctaatac gtatatgatc aaagtggcgt taaagatgtt aggactagac
     1561 tatacacctg gaatgggact acctagttta gatgaagaag ctaaagctta tcagcaattg
     1621 agagactctt tcaaagagtt tggtcttggt acaacgactg ggatcgattt gccgaatgaa
     1681 tctcctggga tctcacgttc tgttgattat atgaagaagt tcaatgcgga taatggcaaa
     1741 gaatggtatg ctccaggtaa ctttaccgat ttggcatttg gacagtttga cacatataca
     1801 ccaatccaat tagctcaata tgcttcaaca gtcgctaatg gcgggaaacg agtacagccg
     1861 cacctagtca aagcgatcta tggcaatgac gaaaatggaa acttgggtga agtgaagaaa
     1921 gaaattggca caacagtaga aaatacggtc aatatttctg cagaaaatat ggcgatcctc
     1981 cgtgaaggat tccaccaagt tgtccatggt accgatcctt acacaacagc caaaccatta
     2041 gccagtgcga aaatggacct ttccgcaaaa acagggactg cagaaacggt tgcggaaggt
     2101 catccagata ttgcgacagt caatagtaat atcgtggcat acggaccaac agacaatccg
     2161 gaaattgcca tcagtgttgt cttgccgaac ttattagacg aacaagacca cataaacttg
     2221 acgattgcta aagaaataat ggacgcgtat tatgatatgt tcatggcaaa ataaaaaaga
     2281 ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa ttttgtgaaa atagcttctc
     2341 acatttttgc ttaaatttga cttcataccg agaaactggc ttttgaacac cgtttactcg
     2401 gcaaaaaagg ttgtgacaaa a
//
LOCUS       DAZKCZ010000174         2411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000174 DAZKCZ010000000
VERSION     DAZKCZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.178"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..512
                     /locus_tag="ACG8KN_002532"
     CDS             81..512
                     /locus_tag="ACG8KN_002532"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9189527.1"
                     /translation="MLNKRIANKNDYKLVLEIWEKSVKQTHDFLAEADFNFYKAIIPE
                     NLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFIGKRYGSQILIELIETEDIKRI
                     DVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITHLVKK"
     gene            926..1744
                     /locus_tag="ACG8KN_002533"
                     /pseudo
     CDS             926..1744
                     /locus_tag="ACG8KN_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
     gene            complement(1817..2176)
                     /locus_tag="ACG8KN_002534"
     CDS             complement(1817..2176)
                     /locus_tag="ACG8KN_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9189528.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          830 a          335 c          448 g          798 t
ORIGIN      
        1 taaaatatag cggtagcttt atgtttgact aactatgctt gcaaataaag ttaaaattat
       61 aagaaaaggg gaataagagc atgttaaaca aaagaatagc aaataaaaat gattataaat
      121 tagttttaga aatttgggag aagtcagtaa agcaaaccca tgattttcta gctgaagcag
      181 attttaattt ttataaagca atcattcctg agaacttaga tgaggttaaa ctttatttat
      241 gggaagataa cggagaaatt gtcggtttta gtggtgtaag tgcagaagaa ttagttatgc
      301 tatttcttga tccagatttt attggtaagc gatatggtag tcaaatatta attgagctga
      361 ttgaaactga agatataaaa agaattgatg ttaatagtca aaataaatat gcaaaacatt
      421 tttatttaag ccatggattt gaaattgaat ctgaagaaga cttagatggt tttggtaaac
      481 cataccctat aacgcattta gtaaaaaaat aattcatgaa tggtttatta cctgattgaa
      541 catgaataaa agtaatattt ttattttgat ggcctatttc taggttatga attatccaga
      601 tcaaatttgt gtattacatt cagtaataag gtgcttatct taggatcaac ttatgatact
      661 agctgaccaa ggatgtacca gttatagaaa atcagttaaa gtaaaaatga taaaaatctt
      721 atgtcaacac tgatttaggc tgacctgtgg gttaggagaa agacaggtcg gtagctttta
      781 atacaaaaaa tggcttgtat gtatctagat agttaactga aattagaaat gttcttattt
      841 ttaacggagt aagaaaaata ggctgcttta ggtctgaata ctttttcaat ataatgaaga
      901 tatcaaaggc gacaggagtg tttttatgaa taagaatcct cataatctaa agttggtagc
      961 agtagatatg gatggaacat ttgctcgtac tgattacaca tacgatatcc caaggttcaa
     1021 ggctgtttta tcgtgaatga aagctgttga ttgtcaattt gttgttgcaa gtggaaatca
     1081 atactaccaa ttgagatcgt tatttccaaa ttattatgat gaattaagtt ttgttgctga
     1141 aaatggtgct tttgttaaag ataaaacaga ggtactattt tcagccgata tgcctagaaa
     1201 agcgatcgag gtggtccttg atttatgtaa agactatcct gaagtaaaaa atgtgatgtg
     1261 tggtctgaat agtgcctact gtgaaagagg aaaggtgagt caagcatttt ttgatctaac
     1321 caatatctat taccatagat tagaatgggt agctgatttt gcagaagttc aagataaaat
     1381 tttaaaattt gctccgacta tgcctgttga aaaaaccgaa ttttacgttg aattgttccg
     1441 tgaaaaatta gccgggctat tagtacctac cagtagtggt catggttctg tagacttgat
     1501 tctttctgag tgccataaaa ctttcggctt gaaaatgtta gttgagcgct tggggattaa
     1561 tcctgaccag tgcgttgctt ttggagatgg gggcaatgac attgagatgt tggagtattg
     1621 tggtctgagt tatgcaatgg ataatgcaac ggaagctgtt aagcaagttg ctaagcatca
     1681 atgtccttca aatgacgaag acggtgtttt agtgacgctc gatcgattgt tccttaacac
     1741 gtaaacacta ggtaattcat caaaaaacct cgatataaga aatattcgag gttttaaaac
     1801 tatatttaga acagatttac gaagcgtctg atgagtcaaa atagacttgg acattgctat
     1861 ctaaaatacc actttcattc tttaaacgaa ttgtttgatt tgatggagtt acctctaata
     1921 ctaaatgccc attaataaag gtagtatcgt attcagatgt tttaactgtt gcttcttcgc
     1981 aaggtattgt taaatcatat gctggaatat aatgttgtgt ggaaactaat aaaaaatgat
     2041 caccaatcat acagcatccc tctgctgaag aaaattgatt agagccatca tttaaagtta
     2101 acaagaatcc atcctttaaa cgaggtactt gcttaaatac ttctggttgt acttctaaaa
     2161 ataaattatc gctcattttc acgaccacca tcgacttcca cagttgaaat tgacatttta
     2221 tttaacacac ctattccccc ttaactataa ctcaattcta tataaaaaat attctattct
     2281 caaataattg aacttttcaa atcgccaatt tacttcggct ttatcagagc tcattcatat
     2341 caagagcgat taacgctgta aaaattaaac cgaagatctg ataaattttc tctattttct
     2401 acaatattta a
//
LOCUS       DAZKCZ010000175         2305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000175 DAZKCZ010000000
VERSION     DAZKCZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.179"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..474
                     /locus_tag="ACG8KN_002535"
     CDS             262..474
                     /locus_tag="ACG8KN_002535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFP9189529.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            476..2155
                     /locus_tag="ACG8KN_002536"
     CDS             476..2155
                     /locus_tag="ACG8KN_002536"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9189530.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT          735 a          445 c          473 g          652 t
ORIGIN      
        1 tctttcaatc tagttgattt ctatacttat ttgctgtttt atgctagaat aacaattaat
       61 tgttgtatat tttttataca gaaggtacgg ttgaaaaaat atccgaacag ataagcaaaa
      121 acgaggcttg tctctttgac tcgaatccag tattctagtc aaagcatatg aaaaaccagg
      181 tggcttgtcc cctcgttttg tgttgtctaa aaaaatcaac tagctaaatc actgctttac
      241 gcagataaag gagacaaaac aatgatctat aaagtttatt atcaagaaac aaaagtacgc
      301 aaccctaaac gggaagaaac aaaatctctt tacattgagg caaaaagcga tgtagacgct
      361 cgccaacgtg tggaagaaaa taccccatac aatattgaat tcgttcaact tctagaagga
      421 agtcatctag agtacgaaaa agaaaatgct gatttctcat tagtggagtt ttaatatgaa
      481 actttccatc aaaaataacg agacaggcgt ctttgctatt ggtggattag gtgaaatcgg
      541 gaaaaatacg tatggggtac aattccaaga tgaaatcatt ttgattgatg ccgggatcaa
      601 attcccggaa gacgatttac ttggaattga ctatgtcatt cccgactaca gctacatcat
      661 acaaaactta cacaaagtaa aagccttggt gattactcac gggcacgaag accacatcgg
      721 cggaataccc tacttgctga aacaagcaaa tattcctatc tatgctggtc ctctcgcatt
      781 agcgttgatc acaaacaaat tagatgagca tggcttgctt cgagatgcag agctccatga
      841 gatcaatgaa gatacgatca tccgttttag aaagacttct gtcagctttt tcagaacgac
      901 ccatagtatc ccagatgcat tagggatcgt ggtgaaaaca ccttctggaa atatcgtagc
      961 gactggagac ttcaaattcg atttcactcc tgttggcgaa ccagcaaact tgcataaaat
     1021 ggctcgtatc ggggaagaag gagtcttgtg ccttttatct gatagtacaa atgctgagat
     1081 tccaacattt actaaatcag aaaaaacgat tgggcattct attttgaaga tcttttcaaa
     1141 aatcgaagga cgtatcattt ttgccagctt tgcttctaac atcttccgtt tgcagcaagc
     1201 agcagatgcc gctgtggcaa ctggtcgtaa aatcgctgtt ttcggacgaa gcatggaaaa
     1261 tgccatcgtc aacggtcaac gtttaggata tatcaaagtt ccagacggca catttgtaga
     1321 tgcacacgaa atcaatcgct tgcctgccaa tgaaacgatg atcctatgta caggttctca
     1381 aggagaagcg atggccgcgt tgagccgcat cgcgaatggt actcaccgtc aaatccaaat
     1441 ccagccagac gatacagtga tcttttcaag ttcacctatt cctggtaata caacaagtgt
     1501 caatcatttg atcaatttgc tgtctgaagc tggtgcagaa gtgattcacg gaaaaatcaa
     1561 taacatccat acttccggtc acggtggaca agaagaacaa aaattaatgc ttcgtttgat
     1621 gaagccaaaa tattttattc ctgtccacgg agaattccgc atgttgaaag tacatgcttc
     1681 tctagcacaa gatgtaggtg ttcctgcaga aaactgcttc atcatggaaa atggcgatgt
     1741 tttagcgtta actagcgatt ctgcgcgtcc tgctgggcat tttgtggctg gagatgtgta
     1801 tatcgatggt agcgggatcg gtgatattgg taatgtcgtt ttacgtgacc gtcacctgct
     1861 ttcagaagaa ggtcttgtat tagctgtcgc gacagtggat atcaagaaaa aagaaatctt
     1921 ggctggtcca gatattctct ctcgtggatt tgtctacatg cgagaatcag gagaaatgat
     1981 caatgaagct cagcgaatct tattccacgc tttaagagaa tgtttgaatg gacctgactg
     2041 ttcagaatat aaattgaaag aagcgatgac tggcgcgctt cagcctttct tattcgaacg
     2101 tacagaaaga catccaatga ttttgccaat ggtaatgaca ccagatgaag aataaataag
     2161 aggttatgaa atcagcacct tgtcctgaat ataagagaaa acaaatgaaa aacatgagaa
     2221 gccatttttg tttgttctta ttccttgata caaggtggct ctttcatagc ctcttaaatt
     2281 tatttacttt ttcatacaaa aaaat
//
LOCUS       DAZKCZ010000176         2271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000176 DAZKCZ010000000
VERSION     DAZKCZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.180"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..904)
                     /locus_tag="ACG8KN_002537"
     CDS             complement(89..904)
                     /locus_tag="ACG8KN_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9189531.1"
                     /translation="MMKSNVSKLSSKDLRKVSLRYMFGAQLGWNYERMMNVAYVRAVY
                     PALKKLYKDEKELKSVLQSEMQFYNTSPFLSAFIIGIDLALQVEDGSKSKDAVAAIKT
                     SMMGPFAAVGDSLFGAVIPTIFGSLAAYMGLEGNPLGVLLWLLVSLIILFLRYFELPI
                     AFREGKKLVSSVGGLLKDLTESATLLGVVVIGGLVPSVVKVGVPFKLVIGDKKLSFQT
                     EMLDPIMPAIVPVVLVLLAYWLLSKKSLNSTKVIWIFLVLSILMYSLNILAVL"
     gene            complement(891..1703)
                     /locus_tag="ACG8KN_002538"
     CDS             complement(891..1703)
                     /locus_tag="ACG8KN_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9189532.1"
                     /translation="MNDISFFQIFSLICLAILGGIDPYLSGLQLSKPAISGFLAGIIM
                     GDINTGLLVGATLQLMVLGVGTFGGSSIPDFGTGAIIGTALGVVSGKGIEFAIGISVP
                     VGLLLVQLDILARFCGVYFLHRVDKHIEEENFHKISLEAWLSLVPIALSRAIPVGLCL
                     IFGNEVVNTVLNYAPDWLMGGLKLAGAILPVVGIAILLHYLPIRNFFGFLIIGYILAA
                     YLNIPMMGISFAGLAAAIIHYKKLQDKRLLESKLDTINHIEVVNGEVDDDEI"
     gene            complement(1725..2042)
                     /locus_tag="ACG8KN_002539"
     CDS             complement(1725..2042)
                     /locus_tag="ACG8KN_002539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189533.1"
                     /translation="MVFEDQYLQIKNSENIEACIEISNSQESNIFVSPENGPVKPNFN
                     YLTYDRFSQNTVFDGYKLEQSSPAIHSGKKVIDKNGYNLGTDFFGIKLDGILDIGAVK
                     SSK"
     gene            complement(2076..>2271)
                     /locus_tag="ACG8KN_002540"
     CDS             complement(2076..>2271)
                     /locus_tag="ACG8KN_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189534.1"
                     /translation="KKIQIYFIATCCLISLSMLTKVAHAESNPIGNCYYVSSQRGSDT
                     NSGLSISESFKSLNKINELK"
BASE COUNT          814 a          453 c          265 g          739 t
ORIGIN      
        1 atcttctttc cattcattta aaaatataat atttagaaaa ttaaacaggg gacatgccat
       61 aaattagttg atcacgcccc cacatctatt acaaaactgc taaaatattt aatgaataca
      121 ttagtattga caaaactaaa aatatccaaa taactttggt tgaattgaga gattttttgc
      181 ttaataacca atatgccaat aaaactaaaa caacaggtac tatagctggc ataatagggt
      241 ctaacatttc tgtttggaaa gatagtttct tatcaccaat aactaacttg aatggaacac
      301 caacctttac aacactagga accaagcctc ctattactac tacgccaagt aaagttgctg
      361 attcagttaa atccttcaac aatccaccta cactggatac cagtttttta ccttctcgga
      421 aagcaattgg taactcaaaa tatctcaaaa atagtataat gagagatact aataaccaaa
      481 gtagtacacc taatggattt ccttctaatc ccatataagc tgctagagat ccaaaaattg
      541 ttggaatcac tgccccaaat aaagaatcgc ctacagctgc aaaaggtccc atcatactcg
      601 ttttaattgc tgcaacagca tctttcgatt tgcttccatc ttctacttgc aatgctaaat
      661 caattccaat aataaatgca cttaaaaaag gactggtatt atagaattgc atttctgatt
      721 gcaacacact ttttaattct ttttcatctt tgtataactt tttcaaagct ggatatacag
      781 ctcgaacata tgccacattc atcattcgtt cgtagttcca acctagttga gctccaaaca
      841 tatatcttaa actaactttt ctcagatctt tgctacttaa tttgctaaca ttagatttca
      901 tcatcatcaa cctctccgtt aactacttct atatgattga tagtatctag ttttgattct
      961 aataaacgtt tatcttgtaa ttttttatag tgaataatag cagctgccaa tcctgcaaaa
     1021 gaaataccca tcattggaat atttaaataa gcggctaaaa tatacccaat aattaaaaaa
     1081 ccgaaaaaat tcctaattgg caaataatgt agtaaaatgg caatcccaac aactggtaat
     1141 atagctcctg ccaattttaa tcctcccatt aaccaatccg gtgcataatt taatactgta
     1201 tttactactt cattaccaaa aattaaacat aaacctactg gtattgcacg agataatgca
     1261 ataggaacta aacttaacca tgcttctaaa ctaatcttat gaaaattttc ttcttctata
     1321 tgtttatcca cacgatgtaa aaaataaacc ccacaaaaac gagctaaaat atctaattga
     1381 actaataata agcctacagg aacagaaata cctattgcaa attcaattcc ttttccacta
     1441 acaacaccta atgctgtacc aatgattgcc cctgtgccaa aatcaggaat tgatgagcca
     1501 ccaaatgttc ctacacctaa gaccatcagt tgtaatgttg ctcctactaa taagcctgta
     1561 ttaatatctc ccattataat tcctgctaga aaaccagaaa ttgccggttt acttaattgt
     1621 aatcctgaca gataaggatc tatacctcct agtatagcta aacatattaa agaaaatatc
     1681 tgaaaaaaag aaatatcatt catgatcaaa ctaactcctt tatattattt tgaacttttc
     1741 actgccccaa tatctagtat gccatctaac ttaataccaa aaaaatcagt tcctaaatta
     1801 taaccatttt tatcgataac cttcttccca ctatgaatag caggagatga ttgttccaat
     1861 ttatatccat caaacactgt attctgactg aatcgatcat atgtcaaata gttaaaatta
     1921 ggttttacgg gaccattttc aggagaaaca aatatattcg actcttggct attactgatt
     1981 tcaatacacg cctctatatt ttctgaattt tttatttgta aatactgatc ttcaaaaacc
     2041 aatccatttt ctaataaaac tttatcccct ggattttatt taagttcatt tattttattt
     2101 aaacttttaa aagactcaga tatagataat cctgaattag tgtcactccc tctttgcgaa
     2161 ctaacatagt aacaattacc aatggggtta ctttcagcat gagcgacttt tgttaacata
     2221 gataaactga tcaaacagca agttgcaata aaatatattt ggattttttt a
//
LOCUS       DAZKCZ010000177         2242 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000177 DAZKCZ010000000
VERSION     DAZKCZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.182"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..1031
                     /gene="traE"
                     /locus_tag="ACG8KN_002541"
     CDS             699..1031
                     /gene="traE"
                     /locus_tag="ACG8KN_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP9189535.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1650..1991
                     /locus_tag="ACG8KN_002542"
     CDS             1650..1991
                     /locus_tag="ACG8KN_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP9189536.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            2010..>2242
                     /locus_tag="ACG8KN_002543"
     CDS             2010..>2242
                     /locus_tag="ACG8KN_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189537.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDI"
BASE COUNT          799 a          299 c          461 g          683 t
ORIGIN      
        1 tattatgata aaaatcacaa acgtagcctt ttacgtcata agtgttaaaa gaaataatta
       61 atttcaaata tagaaaaggg aacgtatgtt tgcatactcc ttttttttga gttataataa
      121 tacgcgaaga gagataattt cattatttaa acaagctaaa aatcttgata ttacttcttt
      181 tcaggcaata cgaatattat tcgcttacat tttttgtata aatttaattg tctgctatgc
      241 ttgtgatgaa ttgattttcg ttctgcactt ttggatgata aatttggaca attgaaaata
      301 caaaaaaaag aaaggcggta tgaactcgga aggctggtaa ccttcgtcag cgttcataca
      361 cggcattaag atttggccct taatgcgtta acctttcagt gttacggtat tacccgcaat
      421 ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg ttaaggttaa
      481 aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa tgtccgtgtg
      541 gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga tgcgttccct
      601 cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa caagaaaaac
      661 ttgttaaaaa tatttaaaga aattgggtgg tttattttat gtcatataca ggaattttga
      721 gtctcaaaga tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa atcacgaaga
      781 aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata atccagaaag
      841 acaaagtctc agaggaaatg atttattaca ttgggaaaca aaagcaaata attttaaaag
      901 atcctatccc cttaaaagag ctatatccaa caatcaaaca tgtttatgac caaaacggtg
      961 tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt
     1021 taatcagctg aattgatcat tctcataact tgtattttat cgtttttcgc ttgcttgaaa
     1081 atattgtcga gacttctaaa agaacttaga atcattagaa aaataaggag tagacttgat
     1141 ctatcaacaa tttccttcgg ggagtgacgg ggtagatttt tacgattggt aacgtaaaaa
     1201 attggtcaat cgagtaaatg cgcttgtttg gatagtggtg aaatgaattt gtgtaaataa
     1261 tatacacaga gtaaacaaga gggaatttag aggttagaca agttagaata gcgcctagat
     1321 taatcatgag cagctactaa gggatctagg ctttgacgca aactcatgga ataaggagta
     1381 ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag
     1441 atacttgcga ggacgttggt cattggatca atcgagcatt taccgctgtc tactagggtt
     1501 tctcgtttcg gacgccataa acgagtcgat attttttatg tatttttaag taaacgaaaa
     1561 acgataaaat atgaacgagt cctgaataaa gtgaaaaaat tgatataaaa catagagaaa
     1621 atgaaattta cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat atacgattgg
     1681 gcacacatac tattaaggtt tctttccaac gttgggatta caaaggtttt gtgacgttta
     1741 gaagaggcgg aaattgtaaa ggattagatg ttttagcatt aaacgaggat gatttatacg
     1801 atcaaacatt gactgataat ccaattggat tcggcttgct acctgaagat gatgaaggta
     1861 atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct gttgaagata
     1921 cttggtcata tttgagtgat tatattgtca gcgttgaaat cattgaattt gtagcggata
     1981 aagaagaata aaaactggag gaataaatta tgaaattttt cgaagtaaaa tcaccatact
     2041 attatgcgtt aatcgcagca aagaatgaag aagaatgtgc agaattgtat gaacaagtag
     2101 taacagatat cgaaaatcgt gaagaattta ttcgatgttt gcatgagtta aatcgaaatg
     2161 aagcaattca agaggatgcg aaaaaaatta gtgaagaaac gttacaacca atcggtatgg
     2221 aggaagctac aaaagacatt tt
//
LOCUS       DAZKCZ010000178         2232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000178 DAZKCZ010000000
VERSION     DAZKCZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.183"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..437)
                     /locus_tag="ACG8KN_002544"
     CDS             complement(45..437)
                     /locus_tag="ACG8KN_002544"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP9189538.1"
                     /translation="MTVETLTIHQGSDKLEMTTPTGNWVLAREKGYSPVQMLVSAVAA
                     CGGYVYASVLENSKITAEIEKIEVSYDRDDNKKAQPVSAIHIQFYAKIAEADHDKATR
                     CLKLVSPNCPVIQSLDPAIEVTETVTFI"
     gene            complement(434..1324)
                     /locus_tag="ACG8KN_002545"
     CDS             complement(434..1324)
                     /locus_tag="ACG8KN_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9189539.1"
                     /translation="MEELIRVEHATKTFRSKNWRGTVSEVRAVEDVSLTIHRGETLGL
                     VGESGSGKTTLGRSILNLETLTSGEIFFEGKELTALTAEDRKAAYQQMQIIFQDPYSS
                     LNPRMTALELVMEPLYHETKDAARAKAKEILAIVGITGDDVHKLPRSFSGGQRQRIGI
                     ARAVVNRPAFVLCDEPTSALDVSIQAQIIQLLLRLQKEFSLSYLFISHDLSVIRHISD
                     RTAVMYQGKLVELAPTDRLFDNPLHPYTKKLLAAAPIADPVLARGAMEQTEIQSTITL
                     TPLYEWVEVEPDHFVRREKR"
     gene            complement(1324..1800)
                     /locus_tag="ACG8KN_002546"
                     /pseudo
     CDS             complement(1324..1800)
                     /locus_tag="ACG8KN_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(1860..>2232)
                     /locus_tag="ACG8KN_002547"
     CDS             complement(1860..>2232)
                     /locus_tag="ACG8KN_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFP9189540.1"
                     /translation="IWLRKKRDKQAAYAFLKRLIKQFGEPKVLVTDKAPSLSSAFNQL
                     HSEGLFSKTEHRTNKYLNNIIEQDHRSIKKRHKLYQSIRTASSTIKGIETIHALYKIS
                     QRDKSLFGFSVIHEMNTLMSI"
BASE COUNT          547 a          512 c          474 g          699 t
ORIGIN      
        1 actttgtacg cctattggtt tcaggcacac aaagtctttt tcgattaaat aaacgtcacc
       61 gtttccgtca cttcgatcgc tggatctaat gattggatca cggggcaatt tggtgaaacg
      121 agttttaggc aacgggtcgc tttgtcatgg tcggcttcag cgattttcgc ataaaactga
      181 atgtggatgg cactgactgg ttgggctttt ttgttgtcgt ctcggtcata gctgacttcg
      241 atcttttcga tttctgctgt gatttttgaa ttctctaaga cggatgcgta tacatagcca
      301 ccgcaagcag cgaccgcact caccagcatt tggactggcg aatagccttt ttcccgtgct
      361 aacacccaat tgccagttgg ggtcgtcatt tcaagtttgt cgcttccttg atggatcgtc
      421 aatgtttcta ctgtcatcgt ttttctctcc tcacaaaatg atctggttca acttcgaccc
      481 attcatacaa tggtgtcaaa gtaatcgtgc tttgaatctc cgtttgttcc atcgcacctc
      541 ttgctaacac aggatcagcg atcggcgctg ctgctaataa tttcttcgtg taaggatgca
      601 gcggattgtc aaacaaacgg tcggtgggtg ccaattcaac tagtttccct tgatacatga
      661 cggctgttcg gtcactgata tgacgaatca cggacaagtc atgggaaatg aacaaataag
      721 acaatgaaaa ctctttttgc aaacgcagca gcaattggat gatctgagcc tgaatggaga
      781 catctaatgc cgaagtcggt tcatcacaca agacgaacgc cgggcgattg accaccgcac
      841 gagcgatgcc gatccgttgc cgttgtccgc cgctgaaact gcggggcagt ttatggacat
      901 catcacccgt gatcccgacg atcgctaaga tttctttggc tttcgctcgg gcagcatctt
      961 tcgtttcatg atacaacggc tccatcacca gttcaagtgc cgtcatgcgg ggattcaatg
     1021 atgaatacgg atcttggaaa atgatctgca tttgctgata cgctgctttg cgatcttctg
     1081 ccgtcaatgc tgttagctct ttgccttcaa agaaaatctc tccgcttgtt aaagtctcta
     1141 aatttaaaat actgcgtcct aaagtggttt tcccactccc agattcccct actagcccca
     1201 atgtctcgcc ccgatggatc gttagactga catcttcgac cgctcggact tcactgacag
     1261 tgccgcgcca gtttttgctg cgaaaggttt tcgtcgcatg ttctacgcga atcagttctt
     1321 ccattattct gtcacctcct gattgcacca aacgcgatgg gtcttagata actgcgtttc
     1381 ttgcaactca tttgccaatg tctcatgata gtcaaagact tttaattcgg catgcttgtc
     1441 gcctaaatgt gccgcttgca ataattgttt cgtataagga tgcgcaggtt gataaaagat
     1501 ctcttccgtg gtgccttctt ccacgactcg accttgatac atcactttaa taaagtcgca
     1561 cattcctgca acgaccccaa agtcatgggt cacaaggacg acggaaagct ggttctctct
     1621 ttgcaggttt ttgatcaagg ccaaaatctg tgcttggatc gtcacatcta aagccgtcgt
     1681 cggttcatct gcgatcagca atttcggttg cgccaataaa gccatggcga tcatcacccg
     1741 ctgacgcatc cctcctgata actcgtgggg atattgtttc atacgttgta ctggaaaagg
     1801 gttctgttgc aaagttaaaa aattaataaa gagtttccaa ttaatttttg attttatgct
     1861 tagatactca ttaatgtatt catctcatga atcactgaaa agccaaagag acttttgtct
     1921 ctttggctaa ttttatataa agcgtgtatg gtttcaattc ctttaattgt tgaggaagca
     1981 gtccttatgg actgatataa tttgtgcctt ttcttgatgg atcgatgatc ctgttcaatg
     2041 atattattca agtatttatt tgttcggtgt tcagtcttag aaaataatcc ttcagaatgc
     2101 aattggttaa acgcactact tagtgaaggg gctttatcag tgacaagaac tttaggttct
     2161 ccaaattgtt tgatcaatcg ttttaagaaa gcataggctg cttgtttatc acgctttttt
     2221 cgcaaccaaa ta
//
LOCUS       DAZKCZ010000179         2222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000179 DAZKCZ010000000
VERSION     DAZKCZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.184"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..>2222
                     /gene="acm"
                     /locus_tag="ACG8KN_002548"
     CDS             70..>2222
                     /gene="acm"
                     /locus_tag="ACG8KN_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFP9189541.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQK"
BASE COUNT          872 a          348 c          434 g          568 t
ORIGIN      
        1 tgaagaaagg atttttgaga gatgatatag taggttttat ttttagttaa aataggggag
       61 atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat
      121 ataacatcac tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat
      181 gtcacttcgt tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa
      241 ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg
      301 ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa
      361 aataaaaatg ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa
      421 aatattaaaa acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac
      481 gtaaccgata ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca
      541 gctactgtaa atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa
      601 acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat
      661 aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga
      721 ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga
      781 ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca
      841 gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata
      901 atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg
      961 gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca
     1021 gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt
     1081 ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga
     1141 gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga
     1201 gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc
     1261 aattggattg attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat
     1321 gatacagagg gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca
     1381 actaaaattt ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga
     1441 gcaagtacaa acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga
     1501 ataacttcag tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg
     1561 tttaaagtgc aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa
     1621 attgaaaacg ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa
     1681 aaaacatggg aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat
     1741 ctattagccg acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc
     1801 tatcgtttca caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg
     1861 gaagacaacg tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat
     1921 acaccggaaa aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt
     1981 ccagagaaga cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt
     2041 gctcatacag gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct
     2101 aaacagataa caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt
     2161 tttgctgtaa tcattggttt attgatagta actatctgtg ctgggatatt cttacaaaaa
     2221 ta
//
LOCUS       DAZKCZ010000180         2215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000180 DAZKCZ010000000
VERSION     DAZKCZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.185"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..768)
                     /locus_tag="ACG8KN_002549"
     CDS             complement(<1..768)
                     /locus_tag="ACG8KN_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9189542.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEE"
     gene            1259..2215
                     /locus_tag="ACG8KN_002550"
                     /pseudo
     CDS             1259..2215
                     /locus_tag="ACG8KN_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT          773 a          384 c          372 g          686 t
ORIGIN      
        1 ttcttcatgc aacaaagtat aaaccatagc ttgctcatta attttttcta aagtagccca
       61 cgcaggtttc aagatgtgta aatcattaaa acaatcattc cagtaatcaa ccatatctct
      121 ttttaattca acttctacac gccataaatg ttcagacaca acttcaacat ctgcgttatc
      181 tttacgttct tgttttttat tataaattct aataaatcta tcactatcac ggacaccaaa
      241 atattttgtt tctggcttgc cattacgacc ataaaaaaca gttttcttaa ctgctttatc
      301 agtcattgca tagtaatcgc tcaaatcatc ttcaaaatca aaagctaagt ctaatcttgt
      361 aaaaccgtca tcttccatgt agtcgataat attttgtttt aaccaaatca tttcttcatg
      421 tgtgagttta ttgggattaa attcaacacg catattacgt ctatcccaag tatctgcttt
      481 tactttgtca tattcgatat aaactttttc ttgaagtgcc ttagctttaa actttgtttg
      541 aagtatatcc caaagtcgta tttgtggctc tacactcata aagtcagata gctttttagc
      601 attagttttg ttcaaatttc caacgattgt catggcatca aaacttaatg cgggttgaga
      661 ttttcccaaa gtttgaccac ttaaccggct attacttaac cggctattag agacggccgg
      721 tttttttagt gagatttctg cagctaattt ttcatcaatt ttcttcattt tttatcgctc
      781 ctaaaataat ttttggaacg aaaaaaggac gagtggaatc gcaaaataaa tttcgcctgt
      841 atccattcgt ccattttgtt ttgtattcaa aatcaaaaat tgatatacta atcatcgaat
      901 gaataacaag tgtggtttgt tattccgggc gagaacgttg gtagcgttgc tcgtcttttt
      961 ttatgtcttt ttttcgttaa aactatttta aaactaaata tatcttaaag caaataaatt
     1021 caaggggcta aaagcgcccc ttaaaaaccc cttgcctctg aaaagttttg tgtttccttt
     1081 ccgtcggttt caaaagctaa tttactcaaa aaaatagatc aagggtaaat gcactcactg
     1141 cgttcgccct tgagctattt tatttcgcaa attttaacgc atttgccgac gaaaagataa
     1201 aacacataaa aatatacgca gatatattta aaacgtctta gaacgcaaaa ttttacgttt
     1261 aaaagctatg caaggactgt atgaagcaaa attattgagt tatccaagaa cagatacacc
     1321 atttattaca gagaacgaat ttgcttattt aaaagcgaat tttggcaaat atagcggttt
     1381 tttaggactt gatcttgaaa tggttcaaac agagcctaga aagcgttatg tggacggtag
     1441 taaggtacag gaacaccacg ccattatccc aacaaaacaa gtacctaccg aatctgcatt
     1501 agcgaaaatg gacgatttac aacgaaaaat ttatgcttta gtcgttaaaa cgaccgttgc
     1561 catgttttta cctgattact tgtatgaaga aaccaagata caaacgaaag tagctgattt
     1621 gctgtttcaa tcgatcggaa agacaccaaa gcaagaaggg tggaaaattc ttttcaaaca
     1681 acaaaccaaa gaagaaaaag aggacgttca aacgttacca ctcgttatca ttggcgaacg
     1741 tgccgaagtt ggtgttaaga gtgttgaaaa agaaacgcaa ccgccaaaag cttttacaga
     1801 gggtacatta ttaactgcta tgaaaacggc gaataaaacg gttgatgatg aagaagcaat
     1861 caagatttta caagaagttg aggggattgg aacagaagcg acaagagcaa gtattattga
     1921 agcgttaaaa caaaaagaat atatccaagt gattaagaat aagcttgttg taactgaaaa
     1981 aggaaaatta ttgtgccagg cagttgaaag tcagcacctt ttaacgagtg ctgaaatgac
     2041 ggctaaatgg gaaacgtatt taaaaaaaat cggtaaaaga gaaggcaatc aagagaactt
     2101 tattacgaat atcaaaaaat tcattgttca tttactggaa gctgtaccta acgatataga
     2161 aaaactaaat ttttctgatt accaggaaca gaaagaaaaa gaagcagaaa aaagt
//
LOCUS       DAZKCZ010000181         2203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000181 DAZKCZ010000000
VERSION     DAZKCZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.186"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..252)
                     /locus_tag="ACG8KN_002551"
     CDS             complement(<1..252)
                     /locus_tag="ACG8KN_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFP9189543.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKL"
     gene            345..695
                     /locus_tag="ACG8KN_002552"
     CDS             345..695
                     /locus_tag="ACG8KN_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9189544.1"
                     /translation="MLNTLFDAKEIYDSQQKKFSLYPEKFFLVKDLWIAVMQNSSNKL
                     PKTYNHIAFKIDEQDIDSFVSKIQMLGLTVEPGRSRVKDEASSIYFYDYDNHLFELHT
                     GTLQERLKSYNSTT"
     gene            complement(967..1257)
                     /locus_tag="ACG8KN_002553"
     CDS             complement(967..1257)
                     /locus_tag="ACG8KN_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189545.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1465..2157)
                     /locus_tag="ACG8KN_002554"
                     /pseudo
     CDS             complement(1465..2157)
                     /locus_tag="ACG8KN_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          623 a          418 c          324 g          838 t
ORIGIN      
        1 taatttccat agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct
       61 tgaactccat gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct
      121 acttgcataa actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa
      181 gataccccaa atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt
      241 atgtttgttc atttttatac tccttcttat atttcctaat agagtataac atagctttaa
      301 gcattactta aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat
      361 ttgatgcaaa agaaatctac gatagtcaac aaaaaaaatt ttcattatat ccagaaaaat
      421 ttttcttagt taaggaccta tggattgcag taatgcagaa ttcgtcgaat aaactgccaa
      481 agacatacaa tcatattgct tttaaaattg acgaacaaga tatagattcg tttgtctcaa
      541 aaatccaaat gttaggttta actgtcgaac caggacgttc tcgtgtgaaa gacgaggctt
      601 cttcaattta cttttacgac tatgataatc acctcttcga gttacatacc ggtactctcc
      661 aggaacgttt aaaatcctac aattccacta cctgagacta gtaatgacta gtctcttttt
      721 atggcctctg atccccccat tgagcgaata ctaatagggc ttcgaattga tctgttcaat
      781 ctttaagcaa tatccctcaa atgagtttgg aaagtttagt gattcatcac aaatacttaa
      841 atacatgtag ttatatatag attgaggttc ttgtgagcgt atgctcgtgt gggttcaaat
      901 cccatcgtct gaatccatct ttccaaaaca agcggctagg acatttttgt ctcagccgct
      961 tgttttctag tttattcttt catcttgtcg gacctttttt cttgggtata gacttttctc
     1021 atattctacc acaattttgg gtttccctaa cacatgatag gatctcacaa caaattgctt
     1081 tctattatta taataacctg taacgactaa cttcatccct tcgtctactt cagctaggaa
     1141 gttcaaacta tgggaagcaa tcaaacaatt gaccccattt aaactgaaac ggatcaacgg
     1201 tctggcacta acttgcaaag tacggatctt atctactgtt ccatattttt tctccataac
     1261 cttcacctca ataccattat acgaacatac attccctttg taaagaagaa actctgtttt
     1321 atctttttga ctttttgtct attacctttc ttaaaatcat attggcactt cctgcgaaga
     1381 tgaaggacat gtactaaaaa aaagaggcag cagtatagat tactgttgct tctttactaa
     1441 tttaggttta cttgttaaga aagattattt acgcaaatgt tcttcaacaa tttgctgaga
     1501 tttatcagct gtatcttcca cttcatcttt tgctttttct aacttttgaa tatattgttt
     1561 caagcttgag ttttcagcag ttgcttcgtc taatttcttt tgtgtatctg ttaactgagt
     1621 ttctacattt gttttttctt gtgtcagtgt tgcgatttgt tgattcaaac catttacagt
     1681 attttgtaat tctgaaagtt ttgcttgtgc ttcttgtagt cttgctgtcc aatctgcatt
     1741 cagatgattg atttcatcat ttttttagtt gatctcatct agtttgacat tgatttcatt
     1801 ttgcttgctt tgtagttgtg catccttgct gactaattga ctttctaagt tttgtttttc
     1861 tgctgccgat tgattttttt ggttttctaa ttcatttact tttgtttggt attcttggac
     1921 atctgccttt gaatcttgca atgtctgttc tttttgtgct agtcgagcgg taagatctgt
     1981 aatcgcttgc tctttatctg tcaatgtatg atcaagctgt gtcagattct cattgattgt
     2041 atcaatatct gtctctccac cccacaaaat aatttcattt gcgtaccgcg tgcctgtgta
     2101 gccaattaga catagaaatg caacgacaat cccaccagta ataaatttct ttttcatttg
     2161 catagctcct taatccaata cttttacata ctaaaagtaa gtg
//
LOCUS       DAZKCZ010000182         2192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000182 DAZKCZ010000000
VERSION     DAZKCZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.187"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..469)
                     /locus_tag="ACG8KN_002555"
     CDS             complement(<1..469)
                     /locus_tag="ACG8KN_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9189546.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPY"
     gene            complement(613..849)
                     /locus_tag="ACG8KN_002556"
     CDS             complement(613..849)
                     /locus_tag="ACG8KN_002556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189547.1"
                     /translation="MAREVGQQLGLTFSVWTVNELETIEYLARCGVYNITSRCAWAYL
                     WKKGAQNKLFYNQNRGVKRFGDGIHDHMGALFLA"
     gene            complement(921..1145)
                     /locus_tag="ACG8KN_002557"
     CDS             complement(921..1145)
                     /locus_tag="ACG8KN_002557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189548.1"
                     /translation="MANEKATMTINCDLKEEGLEMAVFSLAQTYQSGHKLFLVAMYNY
                     LGLRFGESIYFIILKISSKGKNLGILPKQI"
     gene            complement(1191..1334)
                     /locus_tag="ACG8KN_002558"
     CDS             complement(1191..1334)
                     /locus_tag="ACG8KN_002558"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9189549.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(1817..>2192)
                     /locus_tag="ACG8KN_002559"
     CDS             complement(1817..>2192)
                     /locus_tag="ACG8KN_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9189550.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT          592 a          526 c          334 g          740 t
ORIGIN      
        1 agtaaggaat acccacttgt ttaccatctt tttcagacag tgccatgata ttgtctaaaa
       61 aatgatcttt aaaataatca gcattatccg gatccacttc ttctactaaa gattgaggat
      121 tagtatattt gaaattttct gcaaaataat ctttaaaagc ccatcccact tgtactacgt
      181 ccggtatttc tttggaggca acagaagctt gtaaattttg catcaagcct ttatacatat
      241 caggattata ttttgctaca accttcactt tgtcttgaga ttgattgtat ttatctacta
      301 attcattcac tgtttttcca cctagggtat ccgcattaac atgccaatac tctatcgtta
      361 cacgaccctc cgattttttt gttgtcttgg tattcccact accacaagct gccaaagaga
      421 tgacacttaa aacagacaca acccctataa caagattttt ctttaacata acattaactt
      481 cctcctaatt tctttatggc catttttcta atcaagaaat attatgaact accaatgtaa
      541 atttctagtt aattttctgt aaataattcg aaatttgtgc atttcggggt acagggtcac
      601 tggacatatc catcaagcta agaataaagc ccccatatgg tcatgtatcc catctccaaa
      661 tctcttaact cctctgtttt gattataaaa tagtttattc tgtgctcctt tcttccataa
      721 ataggcccat gcacaacgtg aggttatatt ataaacacca cagcgcgcaa ggtactcaat
      781 cgtttctagt tcattgactg tccatacaga aaatgtcaat cctagctgct gccccacttc
      841 acgggccaac ttagttaacc atccttcatg aatattgacg atttgaacgc tttgataggc
      901 taatttctgt aaggtatcga tcaaatctgc ttcggtaata tccccaagtt ttttcccttc
      961 gaactgattt tcaatattat aaaatatatg ctttccccaa atcgcagccc aagataattg
     1021 tacatagcca ctaagaacaa tttgtggcca gattgatagg tttgtgccag cgaaaaaacg
     1081 gccatttcca atccctcttc ttttaaatca cagttaatgg tcatggttgc cttctcattg
     1141 gccactgctt gaaacacctg ctcaagggtt accgaatctg ggacaacttc ttacctgtca
     1201 tgggctagta ttagcgtacc atctgtttgc tgacgcacat ctacttcaaa acaaagctgg
     1261 cgatccaaac aaaagcgaag aaatgcccag ctattctcct ccgtattgtc actaccagag
     1321 tgtgcagtga tcatggtcat tttgacccct cctttaattc cgtgattttc tcccacacgt
     1381 ccactatacc gatgaagcgt taatttcttg ttaatttctt gtaaattccc tgcgtttttt
     1441 cagtaaacta gttgtaaatc ttgcatgctc cttcctacga actcatgaat cgtcgatttt
     1501 gcccttctag ctagtttcac caattaagta gttctacatt ccccgctttt aacctactac
     1561 accttttttt gttttcagct attccttctt cttgctgaaa gtccttgagg ttctttctta
     1621 caggatacca tcaaggtgtt caatggctta ttcccattga atgaccgaga cttactatca
     1681 gatttctgct cgttgaacca agtctttgat acaggatttc ttccgtcatc ttctcacaaa
     1741 taccaccttg cgtttctcta gcgattggtc gatgtgcacc ctcccagtgg actgtcacta
     1801 cccctttttg ctatgcctag cccacaaaaa aagctgtcct cacagacagc cagactgtag
     1861 acaaaagctg ttccaaactc gtaactcgag cttggaacag ctttttatct acttcgtttc
     1921 gaatttttga acaaaaaaat aaaggctcct tgcctttcca tagccaggtg gctaatttct
     1981 ttaaattcat tgcagcaaac actagcatcg tatgcgtcgc gactttttcc aagcctcgat
     2041 acttggtcca acgcatccca tgcttttctt ttgcatccgc aaatattcgt tcaatcgttt
     2101 gtttccgttt tttgtaaatt gatttattca gatctgttag gcgaagatgt tccacttcat
     2161 ccatcagatc tccccaaaca tgtctcgtga tg
//
LOCUS       DAZKCZ010000183         2192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000183 DAZKCZ010000000
VERSION     DAZKCZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.188"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..252
                     /locus_tag="ACG8KN_002560"
     CDS             7..252
                     /locus_tag="ACG8KN_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9189551.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            328..693
                     /locus_tag="ACG8KN_002561"
     CDS             328..693
                     /locus_tag="ACG8KN_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFP9189552.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(754..1317)
                     /locus_tag="ACG8KN_002562"
     CDS             complement(754..1317)
                     /locus_tag="ACG8KN_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189553.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1885..2085)
                     /locus_tag="ACG8KN_002563"
     CDS             complement(1885..2085)
                     /locus_tag="ACG8KN_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9189554.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          671 a          418 c          338 g          765 t
ORIGIN      
        1 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
       61 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
      121 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
      181 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
      241 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
      301 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
      361 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
      421 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
      481 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
      541 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
      601 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
      661 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
      721 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
      781 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
      841 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
      901 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
      961 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
     1021 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
     1081 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
     1141 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
     1201 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
     1261 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
     1321 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
     1381 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
     1441 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
     1501 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
     1561 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
     1621 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
     1681 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
     1741 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
     1801 ttcagcccat tcaacgacca atgaattttt tcgtacaaaa aaaccgtctt ttctttcgaa
     1861 aagacggtta aaagtcaatt tttattaagc tttaacaatg tttgttgctt gagggccacg
     1921 ttgaccatct tcaacatcgt aagacactgc ttggccttct tctaaagttt tgaagccgtc
     1981 accttggata gctgaaaaat gtgcaaatac atcatttcca tcttcaccag tgataaagcc
     2041 aaaacctttg tctgcgttga accattttac tgtaccgtta ttcataaata aattcctcct
     2101 gatacgtatc aatgtacgtt ttttcgttgc aataaatatt tgataggcaa aaggagtttt
     2161 atctatgtag atatatcgct caaattacgt tt
//
LOCUS       DAZKCZ010000184         2182 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000184 DAZKCZ010000000
VERSION     DAZKCZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.189"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..310
                     /locus_tag="ACG8KN_002564"
     CDS             137..310
                     /locus_tag="ACG8KN_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189555.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            950..1423
                     /locus_tag="ACG8KN_002565"
     CDS             950..1423
                     /locus_tag="ACG8KN_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFP9189556.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="ACG8KN_002566"
     CDS             1432..1659
                     /locus_tag="ACG8KN_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189557.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          707 a          345 c          321 g          809 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tg
//
LOCUS       DAZKCZ010000185         2106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000185 DAZKCZ010000000
VERSION     DAZKCZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.190"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..845
                     /locus_tag="ACG8KN_002567"
     CDS             <1..845
                     /locus_tag="ACG8KN_002567"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9189558.1"
                     /translation="TNMAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIIT
                     GGAKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTF
                     FEDAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVK
                     EKNQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVT
                     ATAMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLS
                     RNWG"
     gene            850..>2106
                     /locus_tag="ACG8KN_002568"
     CDS             850..>2106
                     /locus_tag="ACG8KN_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFP9189559.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILRENGIKED"
BASE COUNT          638 a          289 c          430 g          749 t
ORIGIN      
        1 aaacaaatat ggcaataaaa tcttatatta aacggttatt gctttatatc cgtcatggag
       61 atcccaaaat aaatttagat gtgagttatt tatctgaagg tgcaatgctt gctggtaaga
      121 ctgtgataat tacgggtggt gcaaaaggac ttggttttgc aattgcaaaa tcatttatag
      181 ctcaaggcgc aagcgttatt attacaggaa gagacatgga ttcgttgaat aatgctaaat
      241 gtcagcttgg agatatgtgt tctatcaggg cgtttgatat aactgatgct aaaatgtatc
      301 gaacgttttt tgaagatgca tacgagacct ttggacgtat tgatatactg cttaataatg
      361 ctgggatctc gttacacgaa gatggattta tgagtgttac tgagaaaact tgggataagc
      421 aaatggatac aaatttgaaa ggtgtttatt tctttacaca ggaatttatt aagtttgtta
      481 aagaaaagaa tcaaatgcaa gcaaatatta ttatgatgtc ctcagaaaga ggcttgtgtg
      541 gtgatagtat tccgtatgga ttatcaaagg ctgcaattaa ctctttagtt gtaggcttgt
      601 ctaagaaata tatttctgag ggaattaggg ttaatggtat tgctcctgga gtaactgcaa
      661 ccgctatgac tggtatttct gaaaagggta atttgtataa tcctgcaacg atcggttata
      721 gatattttct cccagaagaa gttgctcaag ttgctttgtt tttggcaagt agtctttcaa
      781 actgtatcag tggtgaaatc atagcctgtg accaggggca atatttaagt cgaaattggg
      841 ggtaataact tgggaatatt attaaaaaaa tataatagcc ttagtgagcc tgcaaaagca
      901 tctatttggt ttacactttg taatgtgttg caaaaaggaa ttctattttt gactacacct
      961 atttttacaa gaatgctttc aactgcagaa tatggtaagt atacgatttt tatatcgtgg
     1021 tattctatta tagcaatttt cacaacgttg aatttatctg caggtgtcta taataaagga
     1081 ctaattaaat acgagaataa atatgcatta acctcgtcat ttttaggatt atcgtcattt
     1141 atttcccttt gtgcattgat gctatacctt ccatttagaa cggttataaa tgggctactg
     1201 gatttatcga cggagtttgt catattgatt cttattgaat caatatttga gccggctttt
     1261 ttgctttggt cagttaagga acgatttgat tacaagtatc aaaaactagt actcatcaca
     1321 gttcttatgg ctgttttaaa tccaggtctc ggtattatat taatcttatc atttggggag
     1381 tatgctattt ggagagtacg agcttatgtc gtggtaaatg ttgtagtggg gatgtttatt
     1441 ggcataggaa tactggctaa gggtaagaaa ttatttgata aagagtactg gacatatgcg
     1501 ctcagattta atttgccatt aataccacat tatttatcaa tgacattgct gaatcaggtt
     1561 gatcgcctta tgataaatag tatggttgga gctagcgagg ctgcgattta tagtatcgct
     1621 tacactatat ctagtatgat gaatatcata acaacagcta taaacggttc ttttattcct
     1681 tttacataca aagcaattaa agatggcgtg tatgaaaaaa ttaaatccgt tgctaatcaa
     1741 ctattagtgt tgatttttgt cttgtgttcg tttactatgc tgttcgggcc agaattgatt
     1801 tgggttgtcg gaggagaaaa atatatggat gctgtttgga ttattcctcc agttgcggca
     1861 tcagtatttt tcatgttttt atatccgctt tttggcaata ttgaatttta ctatgaaaag
     1921 acaaactacg taatgtttgc atcttgtgtg ggctctatcg caaaaattat ttcaaactat
     1981 gttagtattc cccgttttgg atatatttcg gctggttaca cgacattgat ttgctatatg
     2041 ttgttttcct ttgcccacta catgttttat aaaaaaattc ttagagaaaa tggtataaaa
     2101 gaagat
//
LOCUS       DAZKCZ010000186         1990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000186 DAZKCZ010000000
VERSION     DAZKCZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.191"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..312
                     /locus_tag="ACG8KN_002569"
     CDS             <1..312
                     /locus_tag="ACG8KN_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP9189560.1"
                     /translation="QETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKES
                     SGSYTATNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGW
                     Y"
     gene            complement(636..719)
                     /locus_tag="ACG8KN_002570"
     tRNA            complement(636..719)
                     /locus_tag="ACG8KN_002570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Leu,seq:cag)
     gene            1247..1693
                     /locus_tag="ACG8KN_002571"
     CDS             1247..1693
                     /locus_tag="ACG8KN_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9189561.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNLNCQIKLDRSSILT"
     gene            complement(1657..>1990)
                     /locus_tag="ACG8KN_002572"
     CDS             complement(1657..>1990)
                     /locus_tag="ACG8KN_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9189562.1"
                     /translation="ESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQR
                     GLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVS
                     IDDLSNLI"
BASE COUNT          626 a          368 c          329 g          667 t
ORIGIN      
        1 caagaaacgg ttcaaacgga aacacaagca gcgcctgttg ctgaaacaca acctgctcct
       61 gctgtaacag aaactgctgc tactccagca agtacaagct ctgcaaaaga atggatcgca
      121 caaaaagaat caagcggttc ttacacagca acgaacggac gttacatcgg ccgttaccaa
      181 ttagattctt cttacttaaa tggtgactac tctgctgcaa accaagaaag agtagcagaa
      241 caatatgtta cttcacgtta tggttcttgg gaagcggcga aagcattctg ggaagcaaac
      301 ggttggtact aagattcatt atttgaacgt ttaaacacat tcaaaatata gtaagaggtc
      361 atgcgattta ttctttcgca tgacctcttt ttgagtttaa gtttagtctt tattccttaa
      421 ctatcgtttg ccagtatttc ttgtatcaga atctataaat ctactatttg aatcaatcta
      481 ttaggtttta aactgtccca ccttcacaca aatttttttt gatttatttg aatctggttc
      541 cgttgtttaa aatttttcct atattctttt agataatatg ttattttctt tctcatcgct
      601 ccaggtgtac cttctcttta taaaaaaaca aattatgcgg ctaagaagac tcgaacttcc
      661 acgaggtcac cctcactagc gcctgaagct agcgcgtctg ccaattccgc catagccgca
      721 tcacatcttt tagtttagga ttacatgtgt tttttgtcaa gaacatattt ttgttctttt
      781 tcatttctgt acgccctccc ttatgccatc tacacaaata attcatccga gcaactccat
      841 catctattga gggttggtga aaaaatctgt tttatccata tgatcgattt caatgttaaa
      901 cgaacgactc tgggattagt aactatttta tttttttctt catttctttt ttcctctttt
      961 tctcgattca gctatcttct ttcatttgga tattgattat ttcttctgtt gattactatc
     1021 cttctggaat tctagacgct gaagtaattc acgttttctt tcttctagag atcataagta
     1081 acgaatgaac gaacttcaaa taattttttt attgtaattc atatttgtat tgttcgatga
     1141 ttcttaatac gatatgacac aataaataaa aataggcatg gctatttaca acaatagtgc
     1201 tatagtagaa aacaaatgaa taaggttact taaagaggtg aatacgatga aactgatcaa
     1261 actatgggaa gcagatttaa agaaagctta tcagctacaa actagctttg gaccaaatga
     1321 gagtggattt atcaacaatg cttatggcta tacatttgct gaatttttag attatgtaaa
     1381 aaaatgcgaa aaaacgtctt taggaatcga tcttcctgag agttacgttc ccgcaaccgt
     1441 ctttatttta gaaagtgatg aacatgagta tgtaggaatt tttaatttgc gccataaact
     1501 gaacgaaaca ttaagaaacg gcgctggcca tattggttac ggcatctccc ctaattatcg
     1561 taaaaaagga tatgccacac aaggattagc attagtacta caagaggcaa aaaaaatcgg
     1621 cattgatgag gcacttttgt ctgtaaatct taattgtcaa attaagttag acagatcatc
     1681 aatacttacg taactcaaaa atacttccaa aggtgttcga taatccagtg attttctagg
     1741 aatattattt cgtttagatg cgacagattg aataaaaaat tcatctactg aattgaaatc
     1801 catagatttc aataaaccat ctctacgtaa caatccgtta gaattctcgt ttaggcctct
     1861 ttgagacggc gttcctggat cagcgaaata aatggcaata tcattgacat tactgatctg
     1921 tttccaattt gaaaattcct ttccacaatc aaaagtgatg gatttgaata ggtttttcgg
     1981 tacagattca
//
LOCUS       DAZKCZ010000187         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000187 DAZKCZ010000000
VERSION     DAZKCZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.192"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8KN_002573"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8KN_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8KN_002574"
     CDS             complement(728..1744)
                     /locus_tag="ACG8KN_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9189563.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKCZ010000188         1973 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000188 DAZKCZ010000000
VERSION     DAZKCZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.193"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..897)
                     /locus_tag="ACG8KN_002575"
     CDS             complement(124..897)
                     /locus_tag="ACG8KN_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9189564.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGICYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILTELGRSRPSSIGG"
     gene            complement(916..>1973)
                     /locus_tag="ACG8KN_002576"
     CDS             complement(916..>1973)
                     /locus_tag="ACG8KN_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFP9189565.1"
                     /translation="NLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVGK
                     ILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKG
                     DKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLK
                     MEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWL
                     FQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAV
                     LTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLT
                     PVWIVVLQMIHGIGLFS"
BASE COUNT          617 a          436 c          344 g          576 t
ORIGIN      
        1 catgttatat caactcattt cttgtataga ctgattgtaa catgggacaa aataaaaagg
       61 caatacgcct tagttgtcgg tcttcgagtt accaacggta accctcatac cgaacggcat
      121 tcatcaccca cctatagagg atgggcgact tctgcctaat tctgttaaaa tagcttcatt
      181 atcttgaagc tctccacggg ctgtattgat caagatgctg ccttttttca tttgagctaa
      241 aaatccttca ttgatcattt gatcgttttc acctgggata tgaggtacat gaatactgac
      301 gatatcgctt ttggctaata actcttcaag cgagtggaac gtcaatactt ttttagcagc
      361 ttctgtctga tgaatatcgt agccgataac tttggctcct agtcccttaa acaaaacagc
      421 ttccgtcaac cctatcctgc ctgttcctat gatccctact gtgcaatttc tgatttcacg
      481 actaaacatt tgctcatcca ctcgaaaatc tttgaaagat gttcggatcg tcgtatacgc
      541 agtatttctc aaaagcatca tggcaaaagt caaagataac tctgctatcg catttggcga
      601 atacgatggt acacgggcta ctgtcatacc aaaatcagct gccgcttgta gatcgatatg
      661 attgactccc acagtccgtg taaaaacata acaaatccca tattcctgca tttttgcaag
      721 attggtctca tctgctatac aatttcccct caacaataca gcatcatggt cctttgctga
      781 cacgatattg tcatgtgtca acagttcttc ataaagactg agatcataat ggtaaatatt
      841 caatttttca aaaaaatcta tctcgttcgg acgaacaccg taacatgcga ttctcatact
      901 ttcttcctcc atttcttatg aaaataaacc tatgccatga atcatttgta atacaacgat
      961 ccatacaggt gtcagtacca cagcaaaaat agttgacagt aaggatgcat tggaggctaa
     1021 aagtgcctcc ctatcaaaac tgatagcata ggctgctgcc actgttgcag ctggtgtagc
     1081 catcatgata acgattgttg ccagtgcttc aaaattgact ggtagaatat tcgttgccgt
     1141 caaaactgct agaatcacga tattcaatgc aggtacaaat accactttga ctagactgta
     1201 ataccatgaa gttttttcag atgcagcttt tttgaagctt acttcaccta atgttgcgcc
     1261 gattgctagc cacgccaatg gtgaacttaa accagctaaa taatccatcg gctgaaatag
     1321 ccacggtgca gtctggtcaa ttcgcaaaaa cgctacttgg cttatctctc cttctgcacc
     1381 tgtaactgac acttgtggca gatacccttg gaacagccaa atgaaaaggc cggcaaaagt
     1441 agcgataatg attggattga cgaacatcgc tttgatattt ttagcttcca tcttcaagcc
     1501 actcattttt atgtatccat aagaatacaa aaagattcgg tagccaatat tgaaaattga
     1561 ggaatacatc acgccgacag gaccataaat cgcactgacg ataggcgttc cgaaaaaggt
     1621 cgtagaacca aagatcgtca ggacacgcaa cgttgtttgc ttatcccctt tatagcttaa
     1681 gaaaaaaggt tttgaaacta gaataagaat aatataaata actaatcccc aaatcaagac
     1741 attgattcct tgcatcagta attctgtatt gatatcttgc atgaaagctc gaaatgccag
     1801 tgctggcaaa gccactgtta agacgacttt cgatagaatt tttccaacag attcagaaaa
     1861 aatccccttt tttctacaaa caaaccctaa taatataata aaaactgttg aagtgattgc
     1921 actgacaatc gacatatctg tcaaagtcga tcgaaaaatg tcaaataagt tca
//
LOCUS       DAZKCZ010000189         1933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000189 DAZKCZ010000000
VERSION     DAZKCZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.194"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            203..1168
                     /locus_tag="ACG8KN_002577"
     CDS             203..1168
                     /locus_tag="ACG8KN_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9189566.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLKENIYYHTPNDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLEMQ
                     LRRGKATDWHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYR
                     LRKLLKEESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEE"
     gene            1368..1550
                     /locus_tag="ACG8KN_002578"
                     /pseudo
     CDS             1368..1550
                     /locus_tag="ACG8KN_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          651 a          284 c          438 g          560 t
ORIGIN      
        1 aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta atcacactag
       61 ctccgtaact tcttaatcat tatacgaata tttttaggtt tcttcaagac tagagatttt
      121 ttggtaatga atttttaaga gttacgtgaa cttttgaaaa ttctttccag aagtctcttt
      181 tttctttgag gaggaactaa atttgagtaa aaaaggcacg atcataaatg agtgggaaaa
      241 attaacagcc ccctctaaca ggcggttaag tgttgacgat aaggtacacc ccctaaaatt
      301 aagttggtca atcgatcgaa ttacaatcgt cggaaaactg aaagaaaata tttattatca
      361 cactccaaat gatgtgttga ttttagattt tgaacagctt atgcgcctaa atgaaggtaa
      421 cggttattta agatctgtta gtaataatgg ttggcagttg ttagaccaac atgaagagaa
      481 tattgcttat attgaaattt taaaatggca agaggggaaa gggcgtatag acttcaatcc
      541 gaataagatt agtcaattct tagctagttc catgaaaaat tttattcatg atttgtttat
      601 cgaacctcac ttttcacgtg cagatattgc ttgcgacatt atcgatgtac ctgatgagtt
      661 tattacacaa tatcgggtag ttgatccagt ttcttttaag cctatttatg gacggaatgg
      721 aaaattagag acggcatatt ggggatctcg ctcttctgaa cgtcaaattc ggatgtataa
      781 taaaaaactg gaacaagaga agaaaagaaa aattgtgcca aaagaaatcg tgagttggtg
      841 gcggttagaa atgcagctaa gacgtggcaa agcaacagat tggcatgcga tggttcggga
      901 aagtttagat agctttgcaa gtcctcatta cttgcctgga gatgtaaaac ctgttgatag
      961 aattatgata aaagggctga atcaagatca ttcagagtgg gcgtatatta gcagaaattt
     1021 gaaatataga ttgcgcaaat tgttgaaaga agaaagccaa aatgatgaat tgacgaatca
     1081 tttgcgagaa acctttgcag aatcggcaga cgatttaaaa aaagaattag acacatggct
     1141 tctagggcta gacgtaacag aggagtgata ccgaataaga aaagtcagat gagtgagagt
     1201 aaatttacgt caatgtttaa tgacgtgagt ttacgataag cgattatttt gcgtgaagca
     1261 aaataaagcg taaaaggagt tgagagcgtg acacgctttc ggctcctttt ttcgttgtca
     1321 cttgtgacag cgaataccct cggagagcat ttggaattgt gacagccatg tcacaattag
     1381 gaaaatgtta ctctttgtca aagagtaaca gaaaggttta cactcttttt attggcgaca
     1441 gcgcgaagga ggggtttaac ttggggttta gtatttcgtt caaaaaggga acaaaaaata
     1501 caagcatttc tcataataat cgtgagttaa ctgatagcca aaaaatcaat ggtttgtctg
     1561 gtagaaaata attagcagat ttacaagtgg gaaataaaaa gctgaaagaa gaaaattgca
     1621 tttcaaacta cattttctac aatagctcta aaatggcata ttttgcgttc tgagcggttt
     1681 tagcgtttat ttcgtttagt tatcggcaca atcggtgaaa cacgttagag gagcgaaaat
     1741 ggagcctgta aggcgactgg tagcggaacg gtcgtgttag attgtgtagc cgatagctga
     1801 acgaaataaa tgctggcagc gccattctat ttcggcagga ggaggctcaa ggggttcggg
     1861 gaatgaaatt ccctgaggtc tttttatttt ttctagagga gtgctataat caaagaaaat
     1921 gagaaaaaag agt
//
LOCUS       DAZKCZ010000190         1930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000190 DAZKCZ010000000
VERSION     DAZKCZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.195"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..497)
                     /locus_tag="ACG8KN_002579"
     CDS             complement(147..497)
                     /locus_tag="ACG8KN_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189567.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(490..1815)
                     /locus_tag="ACG8KN_002580"
     CDS             complement(490..1815)
                     /locus_tag="ACG8KN_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9189568.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
BASE COUNT          539 a          417 c          301 g          673 t
ORIGIN      
        1 gtatttatgg tatcctgctg cagcttctac atagaaagag aagcgttcaa caccaacctc
       61 atttatcaaa cgctggccat aaccccaaat ctcttctgat agctgatcaa aggttatata
      121 ctttagctca tattgctgga taatgttcat tctaatcgac cccatttatt atcctggtaa
      181 aattcgatat gacgtatttc gtcgtagtga actttgtttt ttccaacgta gatccccagc
      241 tcatcactac ctaagatctt gctaacaata tcttctggat agtatccttc agcgtttcgt
      301 tcttcttttt gaatacatac tgatctattt tttatgactg cagcgttcaa tacttccgca
      361 acctcttcag tcgtcatttg ttcttttgcc aaattttgat tggctcgttg ttcattgtcg
      421 gcatcaattt tttcagtatg atcagataaa taaaaacctg cccactttaa acgtccacga
      481 tcattataat caatcatttt taagccctcc tgcgccgcct gcatggccac caaccaatgt
      541 acttctagta atactacgag cgccttctaa catagaagaa gcatgtacaa tcgacgtaaa
      601 accatatttt tgccgaatct tgtcaataat tttatctaaa ttttcttcgt caatttgctt
      661 ttgtggatca ctaaataaat ccagctgaag cgaatcagtg tacacaagct tgctataggt
      721 tattccaacg tgccttactt cttgtccttc ataatactta tcaaacaaga aaagacagta
      781 attctgcaat tcctttgtat tatctgttat agggatcttc atctggtgtg aaaaacctgg
      841 tctcgtttct aagatcgacg tgccaatgta taaatggaca caacctgttt tacagttatg
      901 ttgcctgata cgaatagcta cttgttccgc catttctttt accaccagtt cgatttcatt
      961 tctcctggta taatctcgtg gtaacacttg tgaattacca tatgattttt cctttgtcgg
     1021 ttctggtggg atcgctatat ctgttcgatc gatcccgttc gcatgaaaat acagctgtaa
     1081 gccaatcaca cctaaatggc tcttaatagt gtatggattc caattagcta agtctttaat
     1141 actcatgata ccaatttggt tgagtctttt tttcattcga gatccaatac cccaaaggtc
     1201 tgtcatttca ggaatattcc atactttatc aggaacattt tgatacgtcc attcagcaag
     1261 gaatccatca ttatgttttg cttcattgtc taaagcaagt ttagctaata agggattatc
     1321 tccgacacct actgtagcaa gcaatcctag ctcttcttta atattctcct ggatcatttg
     1381 agccagcttc tttctccgtt gacttcttgt tccttcagtc gtaaaaagat tcattgatct
     1441 ggtcactttt aagatcgatt catcgatcga gtaaatcaat aaatcttcgt ctgctacata
     1501 ccttctgaaa atattgttta cctgcatatt tcgcttaata tagagattca tgcgtggtgg
     1561 aacgacatgt aacgttttag gaaaaggttg cggtagatct cgtggtcgac tgacattaga
     1621 aataccgtac ttcttctttg cagctggtga agaagctaaa atcaaccctg atcctgtatt
     1681 atccgctcga ctcatgacga ctaattcagt cgttaatgga tctaaattcc tttctataca
     1741 ttcgacactt gcgtaaaagc ttttcatatc gatcaaaaag taatcattta ctggctcttt
     1801 tgaataatcc atcataaata acactccatt cttccacatt atacaaacat acgttcgttt
     1861 ttgtaaagaa gaatcgatta aatgtacaaa aaaagaagca taggtaagtt atttccctac
     1921 ttcttctgtt
//
LOCUS       DAZKCZ010000191         1858 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000191 DAZKCZ010000000
VERSION     DAZKCZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.196"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..830
                     /locus_tag="ACG8KN_002581"
     CDS             <1..830
                     /locus_tag="ACG8KN_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189569.1"
                     /translation="EQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLY
                     LHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHY
                     TINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINH
                     IPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNN
                     LYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(884..1837)
                     /locus_tag="ACG8KN_002582"
     CDS             complement(884..1837)
                     /locus_tag="ACG8KN_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9189570.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEF
                     GHLEGDTIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFK
                     SITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMEFNQ
                     VNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          568 a          317 c          337 g          636 t
ORIGIN      
        1 ctgaacaagc tgattcttta gatattgcta gcaatcagtc gctatttttt tcggatatag
       61 gattttacca cttaaaagga gtattaggag aaaaatccgt tatttttatc aataagacac
      121 tctcttggct ttatcttcat gataaattgg atcaaaaata cttgagaaaa aatgaagatg
      181 aaacaatcga atggttgaat gaacgattca ttgaaagttt tgaaaatcag tttaatctgt
      241 tgcgagaaga gacgagccaa ttgatccgaa gagagttaac caaaatacat tttaaacttt
      301 tgcattacac tattaataaa gctgatgaat ttccacttga tgtttctttt tttagagaaa
      361 actatccagc attttattgc tatttgatag agtatataaa tgatctaacg tcaaaacgca
      421 aagagttgct tcaagataaa ttttttcttt ttttcagtta tttattatta ttgatcaatc
      481 acatacccat tcaactgatt tctgaaccag taaagatcat catcgatttt tcttatggtg
      541 ctgcctataa tcaatttatc aagaaaaatt taagcttgta cattaatctg aacacggaag
      601 tgatagaggc aggaaagcct gagttcccgg atgttataat cacgaatagg aataatctat
      661 atgaagaaag tgcatctcaa gtagtggttt ggctcgatcc gccaagagca gtagactggg
      721 ggaatcttac aaagattttg ttgactatcc aagaagagaa atacaaagac agcaaaattg
      781 acaagcaaat agacgatttt cctgaagaaa tcatcgacag gatcgaatag tgaacttctt
      841 ttagaaagtc cgactgcttt ttctaaaaat attatttgtc aaattaagcg agacaaaact
      901 ttccatttac gtaactcaaa aaaacttcta atggtgtttg gtaatttagt gattttctag
      961 ggatatggtt tcttttggac gcaacggatg agatgaatcc ttgattgact tggttgaatt
     1021 ccatttcttt tggtagtcca tcttttcgga ggagtccgtt tgaatgttca tttaatcccc
     1081 gttgggaagg cgttcctggg tctgcgaaat aaatatcaat atcattcgta ttactaatac
     1141 ttttccaatt agagaattct tttccacaat caaaggtaat tgatttgaaa agatttttgg
     1201 gtacggattg aagccattca ttaatcgaat tttcaatatc aactgcctta cggccttctg
     1261 gtttcaaggc gataatggct tttgaaagtc gctcaacgag tgtgatgacg gcacttttat
     1321 ggtggatgcc gacaatcgta tcaccctcta aatgtccaaa ttcttcttcg aaaacgacat
     1381 aatctttttt acgttcagaa atatttcttt tgaatgcctg ttttccacgt ttttccttat
     1441 aaccatttgg ttttcgtttt ccttgcatcg gtaaatgtaa aacattgaat tctcccgtct
     1501 taaaccggcg atagagggta cttactgaac aagagaaggt tcgctctgca cggccaataa
     1561 tgacgtctgg ggtccaacct tcagttgatc ttttttcaat gtattctttt tcctcagcag
     1621 gaaaaatgat ttcggttctc ccacaacgtc gcttattttc tttgtattgt tcaaagtatt
     1681 caagtgctgt tccaccacat ttgagaaagt tatagacatt gtaaattgtt tggcgtccac
     1741 gtttaagctg cttcgcaacg atagcaaccg gagtttcttg atgaaaatat gcttctatca
     1801 ttacaagctc gtttggtgta agatgggtat aagtcattta tgttcactct ccttgtat
//
LOCUS       DAZKCZ010000192         1854 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000192 DAZKCZ010000000
VERSION     DAZKCZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.197"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1048
                     /locus_tag="ACG8KN_002583"
     CDS             <1..1048
                     /locus_tag="ACG8KN_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189571.1"
                     /translation="KQSQQDILDYKYLDGYFTANGFNSLEYEYALENPEQFEEYSNSM
                     LELYNNHHALLCDYAGSSEAQGEFSKSRPYYIQQTIIANRNYISEFSNIQVNGKSLNE
                     DIYDTPTILVSRKYKSDETFIKEHITQEYFRLMNYNQFYGIPEKERHINEFNIVYVDD
                     NSTIKVNTENGFSDMSEPIIIIDTGNFAGLYYLDSLNTRNLFFQMDSREEFSSLLAEY
                     GLKQLVTAGTLLTPYLMQLENVTFVLKTLTMFAIVFIISLLFILYISNYVDIFVNRKK
                     YATKEIMGFSHFKTLKNRYIFWGIELIASCILTAINYNFACLFAIILIDYIFCELLYR
                     IYILNALYEIEKGA"
     gene            1051..1701
                     /locus_tag="ACG8KN_002584"
     CDS             1051..1701
                     /locus_tag="ACG8KN_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9189572.1"
                     /translation="MSLITLKNIKKVYGKNQILFENLSLEIEKGEFVGLVGKSGAGKT
                     TLLNIIGLITDISEGIITIDGQQKLSVNSRNAMLLRRYTIGYLFQNYGLIEDESVLWN
                     LKLALEYKKMKKEDKLKVINEYLKQFNLSDMLNKKIYQLSGGEQQRIAIIKLILQGSQ
                     IILADEPTSGLDKENEQVVMKLLKELNEKGITIIMVTHNMDLRNYFSRVINVETFR"
BASE COUNT          696 a          221 c          309 g          628 t
ORIGIN      
        1 taaacaaagc caacaggata ttttagatta taaatatctg gacggatatt ttacggctaa
       61 tggttttaat tctttagaat atgaatatgc attagaaaat ccagaacaat ttgaagaata
      121 ttctaattcg atgttagaac tgtataataa tcatcatgcc ttgttatgtg attatgctgg
      181 tagcagtgaa gcacaagggg aatttagtaa atctcgtcca tattatatac agcaaacaat
      241 aattgcaaat agaaactata ttagtgagtt ttcaaatatc caagttaatg gaaaatcatt
      301 aaatgaggat atatatgata cgccaacaat attagtttcc cgtaaatata aaagtgatga
      361 aacttttatt aaagagcata taactcaaga gtattttcga ctgatgaact acaatcaatt
      421 ttatgggata cctgagaagg aaaggcatat caacgaattt aatattgtgt atgtcgatga
      481 taattctact ataaaagtta atacagaaaa tggcttttca gatatgtcag aacctattat
      541 cattattgat acaggcaatt ttgcaggtct ttactatttg gattctttaa atacaagaaa
      601 tttatttttc caaatggatt caagagaaga attttcttct ttattagcag agtatggttt
      661 aaaacaatta gttacggcag gaacattact tacaccatac ttaatgcaac tggaaaatgt
      721 gacctttgtc cttaaaacac tcacaatgtt tgcgattgtc tttataatat cattactgtt
      781 tatcttgtat atttcaaatt atgtagatat ttttgtgaat cgaaaaaaat atgcaactaa
      841 agagattatg ggattctctc attttaaaac attaaaaaat cgttatatat tttggggaat
      901 agagttgatt gcatcatgta tattgactgc tataaattac aattttgctt gtctttttgc
      961 aattatactt attgactata tattctgtga attactttat agaatttata tcttaaacgc
     1021 tttgtatgaa atagaaaagg gggcataagc atgtcattga ttacattaaa gaacataaaa
     1081 aaagtatatg gtaaaaatca aattttattc gagaatttgt cgttagaaat agaaaagggt
     1141 gaatttgttg gtcttgtagg taaaagtggt gcaggtaaga caactctttt aaatataatt
     1201 ggattgatta cagacatatc agagggaata attactattg acggtcaaca aaaattatca
     1261 gtaaacagta gaaatgcaat gcttttaaga cgatatacga taggctatct atttcaaaac
     1321 tatggactta tagaagatga aagcgtatta tggaatttaa aattagctct tgaatataaa
     1381 aaaatgaaaa aggaagataa actgaaagta atcaacgaat atttaaaaca atttaatttg
     1441 agcgatatgc tcaataaaaa aatatatcag ttaagcggtg gagagcaaca gcggattgca
     1501 attatcaaat taattttaca aggaagtcaa attatactag ccgatgaacc aacttctgga
     1561 cttgataaag aaaatgaaca ggtagtgatg aaacttctga aagaattgaa tgaaaagggc
     1621 attactataa ttatggtaac tcataacatg gatttgcgta attatttttc cagagttata
     1681 aatgtagaaa cttttagata gtttattaaa gggagatata cttttttgtg caatctccct
     1741 tgttcttttt ctgtcatatc aagactcgtt caataatggt aaattcgtgg taaaatagct
     1801 acttttttag ttgatgaata gcccttaaat actgtattta tgcggataat cgga
//
LOCUS       DAZKCZ010000193         1843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000193 DAZKCZ010000000
VERSION     DAZKCZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.198"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1637)
                     /gene="rny"
                     /locus_tag="ACG8KN_002585"
     CDS             complement(81..1637)
                     /gene="rny"
                     /locus_tag="ACG8KN_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFP9189573.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT          459 a          421 c          319 g          644 t
ORIGIN      
        1 aatgatagca atgttttttc gtgccttaca aaaaaagaag agctgtggct tttgccgcgg
       61 ctcttctttt gtatttttta ttatttagca taatcagttg cacgagtttc ccgaataacg
      121 attactttga tgtgtccagg ataatccagt tcttcttcga ttcgtttacg aatatcacgt
      181 actaaacgaa cagcgtctaa atccgagatt tcttctggct tgaccattac ccgaacttcg
      241 cgtccagctt gaactgcgta acttgattct acaccggcga agctgttcga aatattttcc
      301 agacttctta aacgacggat gtagttttct aatgattcac ttcgcgcacc aggtcttgct
      361 gctgagagcg catctgctgc agctacaagg acagagataa cagaagtagc ttccacatct
      421 ccatgatgag aagcgatcgc gttgattact actgggtttt ctttgtactt ggcagctaat
      481 tccgcaccga tctcaacgtg agatccttca atctcatggt caagtgcttt cccaatatca
      541 tggagcaacc cggcacgttt tgctaattgg atatcttcgc caagctctgc tgctaagata
      601 ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt tttgtccata actagttctg
      661 aatcgcagac gtccgatgat cttgattaga tctggatgaa gtgtatgagc accgacttca
      721 aacgctgcct cttccccgta ttcgcgaata cgttcatcca tttctttacg agatttctcg
      781 accatttctt cgatacgcgc tggatggatt cgtccatctt gtatcagttt ttccaaggtc
      841 atccgtgcga tttctcttcg gattggatcg aatccggaaa gaacaactgc ttccggtgta
      901 tcatcgatga ttaaatcgat accagtcagc gtctcaagcg ttcgaatatt acgaccttct
      961 cgtccaatga tacgaccttt catttcatca ttcggaaggg taacgactga aacagtcgtt
     1021 tctgaaactt gatctgctgc gcagcgttga atcgctaatg ataataagtt tttcgctttg
     1081 cgatctgctt cttctttggc acgctgttca gattctttta ccattagagt taactcatga
     1141 ttcagctctt cttcagtcga tttcatgatg acatctcgtg cgtcctcttt tgataaacca
     1201 gcaatttttt ctagttcttt ttgttgatct tcaatcattt tttcaacgtc tttttctcgc
     1261 tcttcaatta attgctgttt tgaactaagt gcttcttctt tactttcaag tgagttctcg
     1321 cgtttttcta acgagtcatc tttacgattc agcgtttgtt cgcgttgaat caaacgattc
     1381 tcttgagatt ttaattctaa cttactttct ctcagctcac tttcaacttc agaacggtat
     1441 ttctgatttt cttccttagc ttctaacaat gcttcttttt ttagagtttc tgcttctttt
     1501 tttgcactat cgatgattcc agcagctgat gattgtgcac cagctatttc tttctcgtga
     1561 cgtgactttg cgaccataaa ccctaaacca agaccgacaa ttaaaccgat gatagcgagg
     1621 agaatgttga ataccataaa atccacctcc atactatctt tttcattttt atcatctata
     1681 cagcttctat gataaattat tctaaactcg tcaatatgca tcttcgcata tatgtgtaca
     1741 aaaaacatgc acaactttat tctaatgttt gtaccccgta gtgtcaactt taaataaaca
     1801 aagaaacggc tttctactta ttcattaaac gatggttttt aaa
//
LOCUS       DAZKCZ010000194         1835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000194 DAZKCZ010000000
VERSION     DAZKCZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.199"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..701
                     /locus_tag="ACG8KN_002586"
     CDS             <1..701
                     /locus_tag="ACG8KN_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP9189574.1"
                     /translation="LLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYV
                     TDITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIE
                     IFKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFL
                     FIQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLG
                     SIQKTISKEEGVTN"
     gene            835..1779
                     /locus_tag="ACG8KN_002587"
     CDS             835..1779
                     /locus_tag="ACG8KN_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9189575.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
BASE COUNT          629 a          266 c          346 g          594 t
ORIGIN      
        1 gtttattaat aacaactttt gtttttagtt tctttacaag ttatagtagt cgttttctta
       61 aaactaatac tttaaaaaga tggctaactg cattagtagt aggattgcta agtataattg
      121 cgatgtatta tgtaacagat ataactgctc atgcatgggc acatgttccg catattacag
      181 aagtaaaaaa agatacatct gattcatcag gaacaactag cagcaataaa gttgatttaa
      241 aacgccctga cactgatagc ggggatgttt ctaatttaag gtttgaactt tggaaaagtg
      301 caattgaaat attcaagagt aagccgatat ccggaggttc tccaggctac tatattgaat
      361 atgcacatga taaactacca tatacattaa tgggaaaaga tactttaact gctcataatt
      421 ttattttctt agtgatggct gctacaggag gattaggtct attgacattg ttaaccttct
      481 tatttatcca atgctttaaa gccttgcgtt attgtttcac acaaccaaat ctagtaaaag
      541 ataagcactt ttttgctatt ttaatgattc ttacaatagc agtttcggca ttatttatca
      601 cagaactcat tttggttagt acgataggtt catttatttt ttggggatat ctgggttcta
      661 tccaaaaaac tatatctaaa gaagaaggag taactaattg aaactatttt taggtatatg
      721 aaagtagaaa agaaaactag ttatcccaac ttcaacaaat tttatagttt ttacaatata
      781 aaaatggtaa actaatatta tttgatacga aaatgtgcaa ttggagtgaa tttgatggac
      841 actatattga ttactggcgg agctggtttt atcggttcaa cgctagcgaa ttatttggga
      901 aaagaaaaca aaatcgtagt agttgacgat ttatcaatgg gaaaaaaaga aaacttggat
      961 atggataaac aagtgacttt cattgagggg gatgtatcag attctcaatt gatggaaaga
     1021 atcatgaaag aatatcagtt tgcctatatt tttcatctag ctgctgtagc tagtgtggca
     1081 gattctgtag aacggcctgt agaaacacat cgtgtaaatt ttgacagtgc gcttttattg
     1141 cttgagttag cacggaaata tcaatctaat ttaaaacgat tagtatttag ttcatcagct
     1201 gcagtttatg gtgatgaacc aacactgcca aaaaaagaag aatcagtcat ccgtccatta
     1261 acaccatatg ctatcgataa atttgcagca gaacaatatg ttttggatta ctgtcattta
     1321 tatgatgttc cgactagtgc tgtccgattt tttaacgttt atggtccaaa tcaaaatcca
     1381 aactctccat atagcggagt catttcgatt ctagtggatc gttacaaaaa acaattggca
     1441 ggagaaaaag cggaatttac tttatttggc gatgggagtc agtctagaga ctttgtttat
     1501 atagaagatg ttatacaagc acttttactt gttgccaagg aagaaaaagc tttaggacag
     1561 caattcaatg tcggtacagg aaaaagcact acgctacttg aattgatcca ttcaattgat
     1621 caaatcttag gaacagagct agcgcttaaa tatgaagcgg agcgttcagg ggatattcgt
     1681 gattccttag cggatatttc aaaaattcgt tctttaggct atcagcctaa atttgatata
     1741 ttgagcggaa tggaacggta tttaaagaca gaaatttgat gaaaaaatga ttgcatcttt
     1801 ggataaataa ttctaaaaag taaaacaaga attaa
//
LOCUS       DAZKCZ010000195         1793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000195 DAZKCZ010000000
VERSION     DAZKCZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.200"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..313
                     /locus_tag="ACG8KN_002588"
     CDS             <1..313
                     /locus_tag="ACG8KN_002588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189576.1"
                     /translation="GEFVMTKIYFMLTSNSGVGSTTIFATRARRNIDDLLAFFSQYYD
                     VSANYTKDKDMIDLLVLPEPFGPFNNERNLPVIQIPTILFLERDFEKIKTHIDAYLAK
                     K"
     gene            complement(358..1008)
                     /locus_tag="ACG8KN_002589"
     CDS             complement(358..1008)
                     /locus_tag="ACG8KN_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFP9189577.1"
                     /translation="MPKVAVMAGTPIDTRMGADLLNEISCELLEVPISGNPAQQTYFQ
                     TLPVFEKMHQIEVKIKDLQSKKIDLLLVYCNSLSASIDFDFLAKKYSMKIITPFQVYA
                     DLAQKHERIGVLTANGQGAAGIESVLVQHHSTIRVYNVTNLDWVHAVEAKIHPSIIVE
                     QFGLTDSMSFFMKNQVEAILIGCTHFPYFLTEYQSRTRIPCINPDDALIEKVKSIL"
BASE COUNT          587 a          318 c          264 g          624 t
ORIGIN      
        1 aggagaattc gtaatgacta aaatctattt tatgcttact tctaatagtg gcgttggttc
       61 taccacaata tttgcgaccc gcgctagacg taacattgat gatcttttag cttttttctc
      121 tcagtattat gacgtatcag caaattatac caaagataag gatatgattg atctattggt
      181 gctaccagag ccctttggac cgtttaataa tgagcgtaat ttgccggtca ttcagattcc
      241 gactatttta tttttggaga gagactttga aaaaataaaa acacacattg atgcttattt
      301 ggctaaaaaa taaaacaaaa aaatcctaag ttattgtctg tgtcaaacca atgcttatta
      361 aagtattgat ttgacttttt ctattaatgc atcatctgga ttgatacaag gaattctcgt
      421 tctggactga tattctgtaa gaaaataagg aaaatgagta caaccgatca aaatcgcttc
      481 gacttgattt ttcataaaaa aactcattga atccgtcagt ccaaattgtt ctacaataat
      541 actagggtgg atctttgctt ccaccgcatg tacccaatcc agattcgtga cattataaac
      601 tcgtattgtt gaatggtgct gcactaaaac actttctatt ccagccgctc cttgaccatt
      661 agcggtcaac acaccaatac gctcgtgttt ttgagccaaa tccgcatata cttgaaatgg
      721 tgtgataatc ttcattgaat atttcttagc cagaaaatca aaatcaatcg atgcactcag
      781 tgaattacaa taaaccaaca gtaaatcaat ttttttactc tgtagatctt ttattttcac
      841 ttcaatttgg tgcatctttt cgaagactgg aagtgtttgg aaataggtct gttgagctgg
      901 atttcctgaa ataggtactt ctaacaattc acaggaaatt tcgttcaata gatcagctcc
      961 catcctagta tcaataggtg ttcccgccat tacagccact ttaggcatac tgatcattcc
     1021 ttcaaaatga atttttgcct attatactct aaaaatcaaa tttgaagaat agcgatccgg
     1081 aagttaaact gaaaattcta aagtaattct ttgatttatt tagattcaac ttaacatctt
     1141 tagtgtacta ataaaaaatt cgctcaatat ttctagctat actttatatc ccaatataca
     1201 taagcttccc tatttttaca ggttacaagt gaaaattaaa ctgcttatat tgattgcatt
     1261 taactactaa ggatgatgct tgttgttagt atgtcagaaa aatccaacag tatagttttg
     1321 tcagcacatt catcattctt tcctgtaaaa caaaaagaga gtagacctat tcttccactt
     1381 catcatcgtt atctaaaaca gcaattttct gctgtaaaat agtcataaat tttttctagt
     1441 agccttctct ttaaactcat cataatctca attatctttt tttaagattt gctcaacttt
     1501 ttccctcttg attattgcaa ttcacacctt ataaagtttt aaactaatgg gagacgtcat
     1561 ctagcttaac ttatagtccc tgctccgttt aatctacact tgttatcttg tattattgat
     1621 aaaagataga ctagttacgg attgaaatta ggttgcaatt ctaaaaatcc agtagtctaa
     1681 aatcatgctt attaatttac aatatcttaa aaaagggttt taacttaata acagaaaaaa
     1741 ttattcatgg gcgaaaaaat gattaggatg gcgtaaaagt taaagatctt agt
//
LOCUS       DAZKCZ010000196         1757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000196 DAZKCZ010000000
VERSION     DAZKCZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.201"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..601)
                     /locus_tag="ACG8KN_002590"
                     /pseudo
     CDS             complement(1..601)
                     /locus_tag="ACG8KN_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            679..1718
                     /locus_tag="ACG8KN_002591"
                     /pseudo
     CDS             679..1718
                     /locus_tag="ACG8KN_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          502 a          385 c          374 g          496 t
ORIGIN      
        1 ataaattggg gtgtgcagga gaaatttccc cagcatacga agaatacttt tttcgtctag
       61 aaaatttaac aaatagattt tcctcagtca tcaatctacg tacaactttt tcagaaagct
      121 ttttacccat attttttaaa gcttggtcaa ttctacggta accatacgca cagaagttat
      181 gattgaaaat cgtgataatt aattgacgtt ctttgtaata cttatctggt ttctcaagtg
      241 cttgtttttg ataaaaatag ctgcttttag gcaactggag ctgttgaaga agctctttca
      301 aggtgaactg atttcgtaag gcatcaatca atatcgtttt ttcttgattt gatagacaaa
      361 gaagattgac gcctttttct ttttttagca gttcagccgc tccttcgagt aatgcttttt
      421 cgagctgtaa ttggtgtact tgtttttgta agtcttctac ttcctttaaa agtagctctt
      481 tttgtttttc ctgatttttt aaagtcatac gcaatggaaa atcctttcca agaaccgtgt
      541 ttttccattg atatagagta actctactaa ttccttcaga ttcggctaaa tttttcctat
      601 tatataaatt gagttaaatt gtattcgttt ttcctcctat aggcgtatac taaagaaaac
      661 acttgaggag tggggaacat ggagacagaa cgccaaatcc gtgaattgga aaaagcgatg
      721 aatacgacag acaaccgccg gttgtacgag cgtttcctgg cagtcaagtt ggttcttgaa
      781 ggccacaccc ggaaagaagc cgggcacacc atcggccgcg acgagcatac tgtcagccac
      841 tatgtgaaaa actactgcca taatggaatc caaggtttag tctccaaaaa acaaagcgga
      901 cgaccttcac ggctgacaga tgaacaggaa gcggaactgg tccaaatcgt cgcctaccac
      961 actccgaagg aagtaggttt caaatcccgt gccaattgga cattggcgat tgtgtgtgac
     1021 ctgattctcc gggaatggag ttatggatat acgcaaaaag gcgtggcgga cctccttcac
     1081 cggctaggac taagctggac ccgtccgacg tataccctca aaaaagcgga ttccaagaaa
     1141 caacaggtat ttttggaaga aaccttcccg agcctaaaaa aaaattgctg aatagcgata
     1201 ttgaccacct cctattcgag gacgaaacca tgatccggga ttaccaagcc attggctgca
     1261 cctggtttct ccgcggccac cagcgaaaaa tccccactta tggacaacac aaaggggtaa
     1321 aattgatagg caccttggat tatgaaaccg gtcggatcat ttgtacagaa gaaggggcat
     1381 acgacgcagc ggccttcctc cgttttctgg agcggacgtt gcgggaatac cccaccggaa
     1441 aaatcgtgat ggtattggat aatgccctta tccaccacgc caagctgatc cagtcttttc
     1501 ttgaagaaaa cgctaggcgc ctggcactca tcttcctccc tccctacaga cctcagttga
     1561 acctaatgga aggcgtttgg aagtgactca aggaatcggt catcaacaat gttttttttg
     1621 accacgtcca gaaaatcaaa cagtcggtcc gcggtttctt agccgatgta aacgagcgtc
     1681 cccttgaggt gattgaccga ctgtgcgtgc ggatgtaaag tatttaattc aacctatata
     1741 gttaattgta gcctatc
//
LOCUS       DAZKCZ010000197         1744 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000197 DAZKCZ010000000
VERSION     DAZKCZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.202"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..955)
                     /locus_tag="ACG8KN_002592"
     CDS             complement(80..955)
                     /locus_tag="ACG8KN_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9189578.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(1210..1294)
                     /locus_tag="ACG8KN_002593"
     tRNA            complement(1210..1294)
                     /locus_tag="ACG8KN_002593"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1258..1260),aa:Leu,seq:caa)
     gene            complement(1312..1383)
                     /locus_tag="ACG8KN_002594"
     tRNA            complement(1312..1383)
                     /locus_tag="ACG8KN_002594"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1349..1351),aa:Gln,seq:ttg)
     gene            complement(1393..1465)
                     /locus_tag="ACG8KN_002595"
     tRNA            complement(1393..1465)
                     /locus_tag="ACG8KN_002595"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1430..1432),aa:His,seq:gtg)
     gene            complement(1527..1611)
                     /locus_tag="ACG8KN_002596"
     tRNA            complement(1527..1611)
                     /locus_tag="ACG8KN_002596"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1575..1577),aa:Leu,seq:caa)
     gene            complement(1632..1702)
                     /locus_tag="ACG8KN_002597"
     tRNA            complement(1632..1702)
                     /locus_tag="ACG8KN_002597"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1668..1670),aa:Cys,seq:gca)
BASE COUNT          543 a          363 c          292 g          546 t
ORIGIN      
        1 aaaataggcg caaccaaata atcatagtcc atttactatg tgtcatttgg ttgcgcctat
       61 agatagttac gcccttattt taatagattc gtgctagatg aattgccaaa tagatgattt
      121 cattttcttg aatggcaagt tgaaattgct gttcgatgat atctttaatc tttagtgctg
      181 tacaatacgc tttgctatgt ttaaatgttt cagtaaaaaa accaatattt tctactggcg
      241 tttcttcctg ataaatcaag cgtttaacca ggaatttgat atgaaccatt aagcgatgat
      301 aagtcagtga ttctttatca taaacgtttg aataggattt ttctagatgg gacaaaatcg
      361 tggtcgtcat ttctactatt tttaagctct ttaaactatt aggctctcct agctcagcat
      421 taatgatatg catagtgatg aatcctactt catttgaccc taactcggtt tgatatcgat
      481 gattgattcg ctcgaccact tctattgcta tatcatattc atttggataa aggaatctca
      541 tttctggcaa cataattgct gtgacatctc tatcttctct ttttcgttga atagcaaaat
      601 aaatatgatc tgtcaatgta atatagatat ttgaatctaa tggagaggag atcctagctg
      661 ttatcagttc aatcacttct tccgcaaaat ccataaattc gattggaatc tgcgagacta
      721 tctcactgaa tcgtttgatc ttttctttag aatctaatac aaataatttg tcagcatcct
      781 caacatcaaa tggcttactc actttagcag aaaagccaat ccctttcccc atcgcaattt
      841 tttctattcc atcgtcatcg atcaatacag cattatgatt cagctttttc atcacataca
      901 ccgttaattc acctccacta tatgataaaa caaaacaact tgcagactcc ttcatacata
      961 tttttgtctc caaacaattt ttaaagacaa aaaacgacct aagaaagaac cctttcttag
     1021 gtcgtgccta attgaatagt agcaccctac ataatttaca aaaatagttt aacacataat
     1081 aaaagcgtta tcaatatctt ttttactcgt tttatgataa tacttgtatc gaattgattt
     1141 ggaggtaatt acataaaaac aaaaaaaata tagcaactct ctataagaaa gttgctatat
     1201 tgataattat accggcggcc ggggtcgaac cggcacgtcc tcaacggaca ctggattttg
     1261 agtccagcgc gtctgccaat tccgccacgc cggcaaattt aaaaataata actggggtag
     1321 ctggattcga accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc
     1381 ccaagaataa agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
     1441 gcgttaacca cttcgccaca ttcgccatca taaaagatgt tatttaattt ctcaataaaa
     1501 aactaagatg aagacatctt agtcaatacc ggcggccggg gtcgaaccgg cacgccctca
     1561 acgggcactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caagttgatt
     1621 tattagtact aaggcggtaa ccggatttga accggtgatg aaggttttgc agacctctgc
     1681 cttaccactt ggctataccg ccattatact attatcaatc tacaaaataa agaataaatt
     1741 atag
//
LOCUS       DAZKCZ010000198         1735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000198 DAZKCZ010000000
VERSION     DAZKCZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.203"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1686)
                     /locus_tag="ACG8KN_002598"
     CDS             complement(<1..1686)
                     /locus_tag="ACG8KN_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9189579.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVY"
BASE COUNT          523 a          315 c          215 g          682 t
ORIGIN      
        1 atagaccctc ccatcttttt tcaattcata atagtaagca ttgttggtaa aattataaaa
       61 ttttcggtca gtggtgtata tatctcctgt gttcgatatt cctgacttag tccaaaaatt
      121 caagtctccc catactgtat acccactttt tgttactttc acgcttttat tctcaagcat
      181 agtaatttga gtgtcttcaa agttgtttcc cttataatac cctcgccatt ccgtacatgt
      241 ttcatctgta tatactgaat aaatcggttg gttatctgcg gtattatatg aaatactaat
      301 gtaaaatact cggcctcttt cagtttttcc ttttgaataa tttacatcta aagaagacca
      361 aaaatcttca ttattaacac taaatgtaac atacttatta agctttgttg ttgtaaggtc
      421 tttgacagcc tcagcattga tataaccata aaattttcca gatttataaa gcttgtaata
      481 gacagcatta ttcgatacat tggttaactt taatctagct gaaaatacag aaccaatctg
      541 aggcttttca atttcctttt cccattgtag attattccaa atcgaccaat ttttagtaac
      601 agttacgttt ttattaattt cttcaatagg agtataggct aaactaacat gtgcttttaa
      661 ataccctaac cattcagagg aatcctgatc tttataaata gaatatagat actttccatc
      721 ccctaaatta tatgctcctt taacaaaata tattttatct tttgtagttt gtcctttact
      781 tatagtacac tctagatttc cccaagttgg ttgaccacta tctttagaag ttaaatatct
      841 gtttatttga acagaggtca actctttaac agcataaaaa ttaatgtatc catatacttt
      901 attacttact aatatttctc catatttatt attattttct ggattagtat aaaccttccc
      961 tatcttgtaa ttatctccaa taatagcttt cttaatttca tgactaaatt gtaaatcatt
     1021 ccatattgac caagcttttg taacagttac cattttatta tagtcaatat aagggtagtc
     1081 aggttcatca ggcaaaattc tttttgcaaa gttaggataa aaatattgta tgttaacctc
     1141 ttttacagtt tctccaggtt gcggtgcatg aatcattaca ccgttacctt tatatatacc
     1201 tacatgataa gttgcacctc gattgccgta aaataataaa tcaccaggct taagtgaatt
     1261 taaacttacc tctgtaccgt attgctcttg attggtggtt cccataggca gttcaatatt
     1321 tacagcttgt ttgtaaacat atttcactaa accaccacag tcaaaagaat ttggtcccga
     1381 tgctccccat tcgtatggct ttccaatttg ctccattgct ttattaatga ctttggtttg
     1441 attagaagtc atagtataat ttactattct tgtttcacta ttttcatttg ataaatttgt
     1501 aggtatttcc ataatctgct taaactgttc ttcactataa ccaactttta aagctttttc
     1561 tttttctata ttatactgtt cttctgtgta gtttatttgt tctgaaaaaa caatagtatt
     1621 gtagtacaca ctcaaaacga ttgagaaaga catcacaaga aataaagtta atagtttttt
     1681 cttcattttt tacctcttct ccttattttt tacaagcgct tgcattttaa tatta
//
LOCUS       DAZKCZ010000199         1705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000199 DAZKCZ010000000
VERSION     DAZKCZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.204"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..190
                     /locus_tag="ACG8KN_002599"
     CDS             <1..190
                     /locus_tag="ACG8KN_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP9189580.1"
                     /translation="LYEVFYSVFKDEIGSDLKKIELIEALLFLSMIPLHGESLNHQMA
                     MLATGLEILGRVVPDIYC"
     gene            278..676
                     /locus_tag="ACG8KN_002600"
     CDS             278..676
                     /locus_tag="ACG8KN_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP9189581.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            701..1417
                     /locus_tag="ACG8KN_002601"
     CDS             701..1417
                     /locus_tag="ACG8KN_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9189582.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
BASE COUNT          536 a          228 c          366 g          575 t
ORIGIN      
        1 tttatatgaa gtattttact ctgtcttcaa agatgaaata ggttctgatt tgaaaaaaat
       61 tgaacttatt gaggctttgc tgtttttaag tatgattcct ttgcatggag aaagcttaaa
      121 ccatcagatg gctatgttag caactggttt ggaaatttta ggacgagttg tcccggatat
      181 ttattgttaa tgctttgagc tattgttttt ctatttgatc agtgtaaagc cgggcatata
      241 aattgcctgg ctaaaataaa ggagatgtct tagttatatg tttaaagatt acgcatttgt
      301 ttttgatatt gatggaactt tgtgccccat caaaaagaaa gatgagaaat atgaagattt
      361 agttccgtat gctaatatgg tggagaagtt gcgatactat aaggagaacg gagcaaagat
      421 tacactgttc acatctcgca atatgaattc ttatcatggt aatttaggtg tgatcaatgc
      481 aaatactgca aaaatcttgt tggcttggct agataagtgg gagattccgt atgatgaaat
      541 tctttatgga aagccttggc caggccataa agggttttat gttgatgatc gcacagttcg
      601 tccggatgaa tttttatcgt gtaccctgga tcagctcgat gagatttgca aaaaatccaa
      661 attgaaatct gagtaaagga gactttataa agtgaataag atgacggtta ttattacaat
      721 ggctggtctt ggcagtcgct ttcgtaaagt tggctacact gtgcccaaat ttatgattga
      781 agcaaaaggt aaaaccttat ttgattggtc cttagacagc ttgcttgatt ataattcaca
      841 tgtagaaaag tatgtttttg ttgtgcgtaa ggaagatgat gctactgttt ttatcaaaga
      901 acattgcagt ctgtatggaa ttgaaaatgt cgaggtagtt gaaattgact atatgactga
      961 tggacaagcc actacatgta tgcttgcaat tccttattgc gcacctgata atgcaattat
     1021 ggtttataac atagatacat atgttgagcc aggtgagatg aaatacgttg atatttctgg
     1081 agaaggacat attccgtgtt tccatgcgga cggtgatcat tggagttttg ccaagttgaa
     1141 cgaatatggt gatgtagtag aagtaagaga aaaagttaga atttctaaca attgtacttt
     1201 aggtgcatat tatttttctt ctgcaaaatt atatgcatca ctctacgaag aatattatgc
     1261 agatgaaagg catctggaaa agaatgagaa atatattgcg ccgctatata attatatgat
     1321 agagaagggc atgaaggtaa ccattagtat agtagataaa tcaaaagttc acgtactcgg
     1381 tactccagaa gagctcgatg tgttcttaaa tgaataggga aaatataatc atagaaaatg
     1441 ttaattataa agctgcgtgt gtgggggagt actattcgat agatattcta aaaatactaa
     1501 tggctatttt agttgttgtt cgtcatttag gtcaaaacta tttagctcca gattaagcat
     1561 ttagtattgg tattgttgca ggactgagta cggttgccgt tcctactttt ttcatcatca
     1621 gttccttttt gttgtgtagt ggtgaagtta catccgctaa attatctaaa caaatctaca
     1681 gagtacttaa aatttacatt atttg
//
LOCUS       DAZKCZ010000200         1678 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000200 DAZKCZ010000000
VERSION     DAZKCZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.205"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            246..1538
                     /locus_tag="ACG8KN_002602"
     CDS             246..1538
                     /locus_tag="ACG8KN_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9189583.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVHTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          545 a          287 c          340 g          506 t
ORIGIN      
        1 tataatgtgt atcaatatta aaactgaaaa agatatggat aataggatca aagccctcat
       61 tggaacactc aatatattat cagatttaaa tttcaaatag ataatttcat tgtttagtaa
      121 tatcgaaaga tactttttta atgatgagag tgtaatataa accgtgtaag taaccattaa
      181 tcctaggcag gtggttgctt cgcggttttt ctttttctac aataagatca agaggtgatt
      241 acacgatggc tagaaagaaa agaaatcctg atgccgaaaa gttagctgaa tccattctga
      301 atgcctatca acctgaatct gtcgatgaca tgcaagatgc tttgaaagat gtgtttgggc
      361 ccctttttga aaaaatgctt caaggagaat tgaataatca tttaggttat gatgcccatt
      421 ctaaagagcc taaggaacac gataaccgtc gaaatggcta tggaactaaa acgcttaaaa
      481 ccagttttgg tgaagtagct attgatgttc ctagagaccg ggaagcttcc tttgaaccag
      541 agttaattcc taagagaaaa cgagatgtct ccgacatcga agggaaggtt ctttccatgt
      601 atgcacgagg aatgagtcaa cgagatattg ccgcaaccgt cgaagctatt tatggctttg
      661 atatttccca tgaaatgatt tcagatatca ctgacgctgt ccttcctgaa ttggaagaat
      721 ggcaagcccg ccccctagca aagtgttatg cctttctatt cgttgattgt atgtatgtta
      781 ctttacgaga aaattatgaa gccaaagaat atgctgtaca caccattctt ggctatgatc
      841 tcaaaggaaa taaggagatt ttaggattat ggttaaatca aacagaatct aaaaatcgct
      901 ggatgcaagt atttgatgaa ctgaaagcac gtggtgtcga agatgtcttt ttcatttcga
      961 tggacggtgt ttctggcctc gaagaaggcg caaaagcgat ctttccatcg gtaattgttc
     1021 agcgctgtat tgttcatctt gttcgaaatg cattgcgcta tattccaagt aaggactata
     1081 aggaagtctg tcgagacatg aaaaagttct acggtgcttc ttctctaaac gctgcacatg
     1141 ctgcttttga cagttttcaa aatcggtggt ctcattattc tggtgctgta gatgtctgga
     1201 aacggaactt tgcacatgtt gaacaactat ttgattatgg tagtgccatt cgaaaaatta
     1261 tgtacactac caatgcggtt gaaagtgtcc actccagctt cagaaaagtc acaaaaaaag
     1321 gagcgttctc taatgagaat gcactcctaa aactacttta tttacgcacg aaagaattac
     1381 acgcgaagtg gtcaggtggt cgaattcaaa actgggctat ggtgcttaat cagttaatga
     1441 tcaacgagac cttctcctct agaatgaaaa gtatgcgatt taccttccgt aacaacggta
     1501 ggtttattca actatgtgtc cgatcaaaat ttatctgatt tcaaaatagc cgagagaatt
     1561 atggtggtta aaaactctta actatttaaa ataagtgtct cgagtttcat tgggaacaga
     1621 gatttacaag aaaaactaac ttaataaaat tcgataatca tgtgaattta taatataa
//
LOCUS       DAZKCZ010000201         1658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000201 DAZKCZ010000000
VERSION     DAZKCZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.206"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..111)
                     /locus_tag="ACG8KN_002603"
     tRNA            complement(38..111)
                     /locus_tag="ACG8KN_002603"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75..77),aa:Ile,seq:cat)
     gene            complement(300..1319)
                     /locus_tag="ACG8KN_002604"
     CDS             complement(300..1319)
                     /locus_tag="ACG8KN_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9189584.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1330..1536)
                     /locus_tag="ACG8KN_002605"
     CDS             complement(1330..1536)
                     /locus_tag="ACG8KN_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9189585.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          465 a          349 c          290 g          554 t
ORIGIN      
        1 aaattctaac acagaacgat tcaaatggct acgttaatgt accctgtagg actcgaacct
       61 acgaccggac ggttatgagc cgtctgctct gaccaactga gctaagggta ctggttgttg
      121 ccacataaag ccataaacaa tcaaccagta gaatgtgtgg caacaaacct gttatcgcat
      181 agcttggagt gtgactatta tgggtgatag tgaagatatg cgataacatc actattttat
      241 cgaatgattt ttatagttgt caatatagtt atgtaatgct cctcaacgag gagctatttt
      301 tatcgtttag gaatatttaa ataccaacgt ttgtcatgga aatcttgcgc accgccttta
      361 gtgtttccct ctggatcatt cgttgcccgc atcatgacat agactttctt attagggaag
      421 ttacgcatgt taaaagatac atgatagcca acatttccag aagtattata agcttgattt
      481 acatctgatc tataaattcc atcagctctt actcgagcta attctttccc agtattgtaa
      541 tccataatga agatatactc gtatttatag ttagcaatgt gccatccagc tacatgcaaa
      601 tttgcatttt cgatttctcc aaactgatca atgtgggcgt aattcgttcc atctgtcagt
      661 gtaggattag ctgcaccagc tcgtgttgga tcaatgacag gcttgttttc agaagttgtt
      721 ggattttcat cggtaaatcc atgagctaaa tcatatgcta atttttcttt acttacgccc
      781 atttcagaaa gataaccgta aggatctgta tgatcgcccc agatgttttg tgttacccat
      841 aaatgcgatt tgattcccgg ttggttataa ggagtgtcca acgttaatgg aataccatat
      901 ttcattgctg aatctctagc caattcaacg tatgccttgt agtttttctc aaacgttgct
      961 ttatcatgtg tgtgttgtaa ctcaatctgc acaggactgt tggcattagc atacgaacca
     1021 gcaccgtact gtacataacc aggttgaccg acttgataaa caattccgcc gtctcccaca
     1081 atataagcag tataagcgct agtccatgaa cgttgcatat actgcgcttc attgcgtcct
     1141 gttgctgttt cattagccgt ttcatgcagt aaaatatact gattatttgc tacttgtgag
     1201 ctaccttcat ttgcgcccaa attaaattca ttgttgatag tataggcaaa tccgttaatt
     1261 ggcaataaaa aaagagccgt taacaggctc atcgcagtaa tagtaatttt ctttttcata
     1321 tctatcctcc tatttatctg atgaaatttt cttatacgtt cgatttgata cacctaaaat
     1381 actccctaga aatgctccaa aacctgtaat aatcatcacc gttatatctg tgtaccgcca
     1441 gttaacacct tttccgacaa gaccgacaaa agtcgctaaa gcggggataa ctactatagc
     1501 gatccatttc aaaatatcaa aggttctatt gttcatacta tccacctccc ttcattggtt
     1561 gggctacttt ctttttcaat cgcttgtttt cttctatcaa tatttctctt tctgaaagca
     1621 aggcatcaat ttgcccgtcc agttgttcaa ttttatct
//
LOCUS       DAZKCZ010000202         1635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000202 DAZKCZ010000000
VERSION     DAZKCZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.207"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..452)
                     /locus_tag="ACG8KN_002606"
     CDS             complement(75..452)
                     /locus_tag="ACG8KN_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189586.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(752..1633)
                     /gene="galU"
                     /locus_tag="ACG8KN_002607"
     CDS             complement(752..1633)
                     /gene="galU"
                     /locus_tag="ACG8KN_002607"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9189587.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          452 a          367 c          241 g          575 t
ORIGIN      
        1 caccaattaa ataaatctct catctcaata gtttatccat tattttatct ttttttgcgt
       61 atacctacta aatattaccg tcttcttctc gttcttctac tttgttcgag agaatctgta
      121 ctagaaaaag tagttgtatg tctacctttt ctgcctatcc gagactttct tttcatatat
      181 gggatataaa tgattagata cagtacagca aaaaataaag caaatacgca agcttcccaa
      241 ctacctaacg ccaattcaac catcaaggta gcaaccccaa cggatgctgc ataccagata
      301 aaaatcagaa acgtttcttt ttttctgacc atgcgaaaca tgataaatag actaataagc
      361 aatagtggca acacaaatat ccaattgctt ccttccatca gccacttgag tatatcaagc
      421 agcgactgtg caacagcgac taattttccc atagtgaaca cacacctttt tttataatta
      481 aaaaattcgc ctttttattc tactatgaat cttctggctt ggctttcatt tctcttatgt
      541 aatcattttc ttttatacaa cttaaaaaaa aattaaacca ttagcataat attttctgaa
      601 tctattcacg aaagtctttt tctatctttc gttttccacg actcaactga tctagagtca
      661 aaaaactaaa acgacagttg cgcaaatgac gcacaagctg ccgctttaat aacaaatagc
      721 ctttcattct cagcaggcca cttatttcca gttaactttc tgttcttagc tttttactca
      781 actggataat atatgcactt aatgaagtcc caatttccga atgttgaagt ccatattcaa
      841 tatttgtctt catatatcct aacttatcgc ctacgtcgta tctttttcct ttaaactctc
      901 tcgcatagac cgcctctgtc tggttaagag tctgaagtgc atccgttagc tgtatctcgt
      961 ttcctgctcc tggttcttga gtttctaata tctcaaatat tttaggagtc agtagataac
     1021 gtccgataat ggctaaatta cttggtgctt ttcccatttc aggtttttct acaaaccctt
     1081 ttactctgca acgacccttt tcgctatctg ttttaggatc aataattccg tatttagatg
     1141 tttcattttc tggtacacgc atgacggcaa cagcggaagc ttgtgtttct tcataatcca
     1201 tcatcaattg ttgagtcaat gggatatcat ctttcatcaa atcatccccc agcatgacga
     1261 caaacggttc atttcctata aatgcttttg cttgaagcac ggcatccccc agtcctcttg
     1321 gatgagattg acggatgaag tgaagattga cttgagtcgt ttcttcaact aaagcgagta
     1381 attctgtttt ttccttttcc cgcaaattgt tttccaattc gatatttgca tcaaagtgat
     1441 cttcaatggg tcttttttct tttcccgtta cgatcaagat gtcttcgatt cccgaatcca
     1501 atgcttcttc cacaataaac tggatcgttg gtttatccac gattggaagc atttcttttg
     1561 ccatggcttt tgttgccggc aaaaaacgtg ttcctagccc agcggcagga atgactgctt
     1621 ttttgacctt cactt
//
LOCUS       DAZKCZ010000203         1631 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000203 DAZKCZ010000000
VERSION     DAZKCZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.208"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..167
                     /locus_tag="ACG8KN_002608"
     CDS             <1..167
                     /locus_tag="ACG8KN_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189588.1"
                     /translation="VLKKKFDFALELIQETSFSLEEIANRIGYKQTTSLFKLFKKYST
                     CTPNEWRNKH"
     gene            463..1407
                     /locus_tag="ACG8KN_002609"
     CDS             463..1407
                     /locus_tag="ACG8KN_002609"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9189589.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          570 a          239 c          322 g          500 t
ORIGIN      
        1 tggttctaaa gaaaaaattc gactttgcgt tggaactgat tcaagaaacg agtttttcgt
       61 tggaagaaat cgctaatcgt atcggatata aacaaacgac ttctttattc aaattattca
      121 agaagtattc aacatgtact cccaatgaat ggagaaataa acattaagaa gaaaatcttt
      181 cactaaagca tatgaaggaa aattgtttta ggtgcgattg ctgtcttatt atatcgttgc
      241 agcgaatttt tttggattta ttttcagttc taaacttttg gttgtattat caggggatat
      301 aggtgtaaac atttgtcatt tatctgttat gcgtattcag gaattcgttt ccatcaaaaa
      361 ttataataac aactattata attaacatgt tgataaaatg aataaacaag actatacttt
      421 tcatgaaagt aacaaatagt tatgaaaagg agctgtttat tcatgaaaaa aacaagtcgt
      481 aaagtagtaa tcgtcggtac aggttttgtt ggaacaagta tcgcttatgc aatgatcaac
      541 caaggaatct ctaatgaatt agtattgatc gatgtgaatc aagaaaaagc agaaggggaa
      601 gcattggacc ttttagatgg tatggcttgg ggcgatgaaa atgtggctgt atggtcaggt
      661 ggctatgaag aatgtaaaga tgctgatatc gttgttatca cagctggtat caaccaaaaa
      721 cctggtcaat ctcgtttaga tttagttaaa acaaatgcgt caatcatgcg ccaaatcgta
      781 aaagaaatca tgggatcagg attcgacggt attatcgttg ttgcttcaaa cccagtagat
      841 attttgacat atatcgcttg gaacgaatct ggtctgccaa catcacgtgt tatcggtact
      901 ggaacaacat tggatacaac tcgtttccgt aaagaaatcg cattgaaatt aaaagttgac
      961 ccacgcagcg tccacggcta tatcttaggt gaacatggtg attcagaagt tgctgcatgg
     1021 tcacatacaa ctgtcggtgg taaaccagtg tttgaaattg tagaaaaaga ccatcgtatt
     1081 gcccaagatg aattagatgt gattgctgat aaagtccgta atgctgctta tgagatcatt
     1141 gatcgtaaaa aagcaactta ctatggtatt ggtatgagta ctgcacgtat cgtaaaagct
     1201 atcttgaata acgaacaagc tgtattgcct gtatctgctt atttaactgg tgaatacgac
     1261 gaaaaagata tctttacagg tgtgccatca atcgttgacg aaaatggtgt tcgtgaagtt
     1321 gttgaattat caatcaacga agaagaaaaa gcaatgttca gcaaatcaac aagtgcttta
     1381 agagaagtat tgaacactgt attataagaa acaatgaata tgaactaaaa atcctaaaac
     1441 tgcttaacag ctgaaatagc tgttaagcag ttttttattg atatctaata ggtaagcaaa
     1501 taaacagaat ggttaaaatt aatcggtccg aaaatgtatc cgtacatatg aacatatgaa
     1561 atttaatctg ttgattacaa taagcgaatc gttatcaatg tttatctgtg gttaattaaa
     1621 aaatccttcc g
//
LOCUS       DAZKCZ010000204         1612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000204 DAZKCZ010000000
VERSION     DAZKCZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.209"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(515..1129)
                     /locus_tag="ACG8KN_002610"
     CDS             complement(515..1129)
                     /locus_tag="ACG8KN_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9189590.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          543 a          298 c          256 g          515 t
ORIGIN      
        1 caatcattga aaaattgaat ttcattgcaa aaaaaataat aaacacttat aaatatccat
       61 tatctatcct tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca
      121 attttgttat atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt
      181 tgtaaagagt tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc
      241 atatggattc tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc
      301 ttataactca tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa
      361 caagcttaaa atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg
      421 ttaatcctca ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt
      481 atttttttaa ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta
      541 gctgttttgc tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg
      601 ttccgcagat cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt
      661 ggttgtccaa aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt
      721 tgatacttcg tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga
      781 tccactgagc tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa
      841 tgtcttctga tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt
      901 catagacacc tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat
      961 cccaaaattc aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac
     1021 cttgattaat ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc
     1081 ttaatttctc ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc
     1141 gcctagctat acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa
     1201 acagtagtta gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga
     1261 acgaatacgc ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat
     1321 ctacataggg gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc
     1381 attgactatg gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc
     1441 ttagcgccaa ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat
     1501 tgtaagtaat ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt
     1561 atatatatat aaataacata aaaaaaggaa tggcttatcc cgatacaaca ag
//
LOCUS       DAZKCZ010000205         1577 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000205 DAZKCZ010000000
VERSION     DAZKCZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.210"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..393)
                     /locus_tag="ACG8KN_002611"
     CDS             complement(<1..393)
                     /locus_tag="ACG8KN_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9189591.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVF"
     gene            complement(406..>1577)
                     /locus_tag="ACG8KN_002612"
     CDS             complement(406..>1577)
                     /locus_tag="ACG8KN_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9189592.1"
                     /translation="KDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFI
                     PIVSSLAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHH
                     TIYPLFWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMF
                     GLPAACYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAF
                     LDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFV
                     FTFFIKKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACAT
                     RLRVAVTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
BASE COUNT          567 a          302 c          228 g          480 t
ORIGIN      
        1 aaaaacaact ataacagaag tatctttacc agattttatt attttgtcaa aatccacatc
       61 aaccaacgag gtttcaggag taacctcatc attttcttga acacaaatcg aaaaaggctc
      121 tccttgtaat tcaactgtat caattccaat atgaatcaaa taatcaatgc ctgagttgct
      181 ttttatatta atagcatgtt tagtaggaaa tatatttgtt actcgaccag ccaatggagc
      241 ataaattttt gaaccatcag gtatgacggc aaaaccatca cctagtaatt tttgtgaaaa
      301 gacaggatca tttacttctg tgatggaaat gatttttccg gaacaaggtg cataaaaagt
      361 ttcttgtttt ttctttttga aaatttcaaa catattaaaa acctcctaat tatcttttcc
      421 taatatttga ttgatttctt gacttaataa gtcagctttc cctccaaaaa cagcttggat
      481 gccacccttc acttcgaaga cagcagttgc accagtgtct tttattgttt gtttttgaat
      541 aatctcccca ttcgtaacag caacacgcaa acgagttgca caagcttcaa cagactcaat
      601 attttctttt ccaccaagag cttcaataat gataagagac tcttcatgta atgatgtatt
      661 tttgtttatt actttttcag aatgaacttc tgattcttct tccattccag gaatagctac
      721 cttaaatttt ttgataaaga atgtaaaaac aaagaaatat ataaatgccc atgcaatacc
      781 aaaaggaatt aattttaacc aattagtttt gtcattccct tgaagtacac cgaataataa
      841 aaagtcgatc aaaccacctg aaaaggaatt ccctacacga atatttaaga tatcagcaaa
      901 ataaaaagat aatccatcta agaatgcatg aatcacataa agccaaggag caacaaataa
      961 aaacatatat tcaactggtt cagtaattcc agttaaaaat gatgtaaggg ccccgctaaa
     1021 ataaagccca ccattctttt tacgattttt cttaggaatt gcacgataca ttgcataaca
     1081 tgctgctggt aatccaaaca tcattgttgc aaatcttcct gcaaaaaatc tagtaccata
     1141 agtaaacaga ccagtgtggt ttggatcagc tagttgggca aagaaaatat tttgggctcc
     1201 agcaattgtt gatccagcta cagtttcggt accacctaaa gatgtatacc agaacaaagg
     1261 gtaaatggtg tggtgtagtc caacagcacc tgttaaacgc aataaaaaac catataaaaa
     1321 agttcctaaa ctacccattt gggcaatttg ttcaccagca actactaaac cattttgaat
     1381 tggtggccaa attaaataaa agaaagaacc aataatgatt gcagctaaac tagaaacaat
     1441 cggaataaat cttgatcctc caaaaaaacc taagaattgt ggtaattcaa tttttctata
     1501 acgattgtgt aaaaatgcaa cagtaccacc aacaacaatt gatcccacta cacctgtatc
     1561 tagtgttgca tcttttg
//
LOCUS       DAZKCZ010000206         1563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000206 DAZKCZ010000000
VERSION     DAZKCZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.211"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACG8KN_002613"
     CDS             complement(52..1518)
                     /locus_tag="ACG8KN_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFP9189593.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          513 a          329 c          290 g          431 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atc
//
LOCUS       DAZKCZ010000207         1546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000207 DAZKCZ010000000
VERSION     DAZKCZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.212"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..79)
                     /locus_tag="ACG8KN_002614"
                     /pseudo
     CDS             complement(1..79)
                     /locus_tag="ACG8KN_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            161..937
                     /locus_tag="ACG8KN_002615"
     CDS             161..937
                     /locus_tag="ACG8KN_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9189594.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(1035..>1546)
                     /locus_tag="ACG8KN_002616"
     CDS             complement(1035..>1546)
                     /locus_tag="ACG8KN_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9189595.1"
                     /translation="TASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILI
                     EQLEWLKKKKIKMAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLK
                     ASEQLRIPVEECLVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLR
                     QEVQVKFGI"
BASE COUNT          546 a          228 c          229 g          543 t
ORIGIN      
        1 gagtggtaac ataaggattt ttacttaaaa cagaatgaat ttgttttctt tcaaaagcgg
       61 gcattggtta gtcaaagact agttcattta tatttatatc ttaatctttt attttaataa
      121 attgtgatat aatgaagtat aactttttag ggagtgtact atgaattttc aagataatag
      181 accattatat ctacaagtag aagcttctat acgtaacgat attcgtgaaa aaaaatatct
      241 tccaggagag caattaccta cagaagaaga attatgtaaa atttatggag taagtaagat
      301 taccattcga aaagctttta aacttttaac agaaagtgga acagttgaac gattaagggg
      361 gaaagggacc tttgtaactc aaaaaaaaga aagtttaaaa ttagctggag ttcatggatt
      421 tagcgattct ctttctaccc gaggacataa aattagatat actgttttac attctaaaat
      481 tataaaggct gatgagtttt tagctaataa attacaaatc aatgtaactg atagagttta
      541 caatattaaa cgtttaatgt gggaagacgg tgccccgatg ggaattgata attttttcgc
      601 atcagaagaa aaatttccaa ctttatttga aaaaggtagc caatatactt cattatataa
      661 acttctagaa gatgactatg atgtcaaagc tacgagctct attatggaaa taaacggttt
      721 atctgcaaca cctgagcttt ccgacttatt gcaatgtata gtaggagatc cgctatttat
      781 tttagaaaag gttggcaaag ataaaaataa tcaaccattt cattattctc aatccacagt
      841 tcgttgtgat agaattagtt atgttattga agtcacagaa aatatcactt tagatacggt
      901 gcataaaaat gattcagatc ctaaagtaat agattgatat taaaagaaat catacccaaa
      961 gcaatcaatt ttgattagat ttgggcatga tttttatttt tactaatatt ctctaaatac
     1021 atcaaaaaac ttatttatat tccaaatttc acctgtactt cttgtcgcag atatttaata
     1081 tcgtaaataa catcatctgc tagacttaca tcttgagaaa aacgtttatc tttaattgca
     1141 agaaccttca tatttgctct tttaccagct tttataccat tataagaatc ttctatcacc
     1201 agacattctt caactggaat tctgagctgt tctgaagctt taaggaatat agtaggatta
     1261 ggtttacttt ctggcaaatc tcttccgcta attaaacaat caaaatatga ctctatttta
     1321 caagtggtaa caacttcatt tatagtatcc attggactgg aagaagcaat tgccattttt
     1381 atttttttct tttttaacca ttcaagctgt tcaattagta tattaggaaa aatatccaag
     1441 taatcaatct tttgcgattt ccaatattta gtaaattttc ccttcaattc taaactgtta
     1501 catccctcat tgtacttttc tatatcatca aaaatacttg ctgtag
//
LOCUS       DAZKCZ010000208         1534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000208 DAZKCZ010000000
VERSION     DAZKCZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.213"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..299)
                     /locus_tag="ACG8KN_002617"
     CDS             complement(9..299)
                     /locus_tag="ACG8KN_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189596.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(417..677)
                     /locus_tag="ACG8KN_002618"
     CDS             complement(417..677)
                     /locus_tag="ACG8KN_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189597.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(814..1227)
                     /locus_tag="ACG8KN_002619"
     CDS             complement(814..1227)
                     /locus_tag="ACG8KN_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFP9189598.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          579 a          253 c          224 g          478 t
ORIGIN      
        1 ttaatacatt attttttatt caaaaaaaat agcacaatgg caaagatcgt aagagagatc
       61 ataagcccca atgaatgact aaaaagaaaa gcagaatcac atgccatatc atcccatatt
      121 tcttcaagaa aataaataag cgggaaaaag ggaaggaata gtagaacatc gatccagcac
      181 catttttttc ttcttttgaa ataacataaa acaataaaca tgaacgtgca gggaagtaaa
      241 aacaaagctt tactcataac taacaaaata ccagctcctt ttattttttt ctcccccaca
      301 ttcaaagagt aacaatggta tgtacttaat aatagaaaaa tggatcaact tgtatgaata
      361 acagctgaaa agatctgcac caaataaaaa acttccagcc aaaactgact ggaagtttat
      421 ttttttctga aatattctaa cacaaatatg aaaaagatgg ccgaagctaa taagccgatt
      481 tcgtagcaaa acaatgagat accagagtcg taataataaa atcctctaat cttatggata
      541 aaatagatca tcggacaagc agataagagt aagtatttgt taggcctttt gaataagtaa
      601 aagaaagcac tggtcattat gataaatggt atccaaaaca aaagtttact taagacaatc
      661 aaatgatcaa ctcccatatt ttttaccaag agagatcata acatctttat cgtaaaaaca
      721 aaatattttt ttgctcattt tgaaaattta aacatactcg ttcttatcca aatatataaa
      781 aatcgcccct cattcatcga gaggcgataa gttctaattt aactcaattt tttttgtttc
      841 ttttggctga taagctgttt taggtaatac caatttcagc acaccgtttt taaaggttcc
      901 agtgattttt tcttcatcaa cattaggaag atagaatgaa cgtttgtaag agaccgaatg
      961 tctttctttc cgtacatagt tgccatcttc tttatcttct gtatgggact catgattggc
     1021 ggagatcgtc aaagtattat ctgaatattc aactgtcgtg tcttctttgt cacagccagg
     1081 catatcagca gttagtttgt attctttttc attttcgact aaatctactt ttggataact
     1141 tgaaacacct aaaaaatcat tgaatgctgg actaaacacg tcattgaaat cagggaacat
     1201 gtcgcgaatg ctaggtacat ttgccatttt tgatcatcct ttcaataagt attttttatt
     1261 tatccctatt tacgtaataa ctctattttg aaaaaaatgc aagtataatg gttagctttg
     1321 tgaatatttc atctaatacg atacaaaggc tatctttcta atttgaaaaa gggatcatta
     1381 aaataataag aagaaaatat gtcaaaaggt aaaaaagtaa attcttctat ccaaatatag
     1441 aaaaattgaa tctagaaaac cgcccgacaa taaatttcat taactcagtt gaccgaaaat
     1501 gaagtagata ccaaaaattt atcaagagag caag
//
LOCUS       DAZKCZ010000209         1532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000209 DAZKCZ010000000
VERSION     DAZKCZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.214"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1342
                     /locus_tag="ACG8KN_002620"
     rRNA            <1..1342
                     /locus_tag="ACG8KN_002620"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..>1532
                     /gene="rrf"
                     /locus_tag="ACG8KN_002621"
     rRNA            1437..>1532
                     /gene="rrf"
                     /locus_tag="ACG8KN_002621"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          428 a          329 c          450 g          325 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg tg
//
LOCUS       DAZKCZ010000210         1530 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000210 DAZKCZ010000000
VERSION     DAZKCZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.215"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..797
                     /locus_tag="ACG8KN_002622"
                     /pseudo
     CDS             1..797
                     /locus_tag="ACG8KN_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            974..1260
                     /locus_tag="ACG8KN_002623"
                     /pseudo
     CDS             974..1260
                     /locus_tag="ACG8KN_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          537 a          274 c          232 g          487 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa gtaaatgcat
       61 ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct acttatattc
      121 ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt atacgagaag
      181 acggcactaa agaagtacta agttatgcga ttgctccgac tgaatcgacc tacgtttgga
      241 atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg tttattaccg
      301 atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct aaataccaac
      361 attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag
      421 aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag gcaaatacct
      481 ttttagggag tatgattgag aagtagcaaa aaacttatcc taaagtgacg cagtcactga
      541 taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc cgaagcattt
      601 actcaactaa tttaatcgaa tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag
      661 agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt gatacataca
      721 atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat acattagctt
      781 caatgtttac tgagtaaccc attattttgc agaaggacga gttatttaca caaaattatt
      841 gacgctccct ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt
      901 attcttgata cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt
      961 aagtcttcat aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta
     1021 aatctgacac taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa
     1081 tattcaccag attatttgga gaaacctctt caacacgaga acaatgatat taattttaga
     1141 tttgtctacg agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca
     1201 agcttccatg aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa
     1261 aaaagaagcg ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca
     1321 taaataaatt gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt
     1381 tcgacgctag cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt
     1441 actcattatt tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt
     1501 taaagtcatc ttccttctcg tttttcatta
//
LOCUS       DAZKCZ010000211         1519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000211 DAZKCZ010000000
VERSION     DAZKCZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.216"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1469
                     /locus_tag="ACG8KN_002624"
     CDS             <1..1469
                     /locus_tag="ACG8KN_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9189599.1"
                     /translation="LTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNLFSDEEIS
                     VSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGR
                     TKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVA
                     ETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQ
                     EEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFT
                     GYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVL
                     EKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEP
                     IFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPV
                     KYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          509 a          273 c          317 g          420 t
ORIGIN      
        1 atcttacaac tgaaatccaa ctattgaatg aaaaggtcaa ttatttaacc aacaaattat
       61 ttggacgctc taaagagact ctctttgagg aaacgaatgg gcaattgaat ctttttagtg
      121 atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca gtaaaaggac
      181 atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata acagagaaag
      241 aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag atgaaagata
      301 tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg gattgtctca
      361 cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt tcttctttta
      421 aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat agtttagtgg
      481 cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa gagaactatt
      541 ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg catattaaag
      601 cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt aatcaagaag
      661 agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa acagatacct
      721 attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat tatcaccacg
      781 acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca gggtatttac
      841 actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc tattgtttcg
      901 cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac acggatattc
      961 ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag aaatggaaag
     1021 acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca ttatttattg
     1081 ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg gatgcagccg
     1141 ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat ggtcgtttag
     1201 aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga cggaaaaatt
     1261 ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg agtgtatata
     1321 aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta tttgataaga
     1381 taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct tggaacaagg
     1441 atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt ctattctata
     1501 ggtcgggtta tttgacgta
//
LOCUS       DAZKCZ010000212         1488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000212 DAZKCZ010000000
VERSION     DAZKCZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.217"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..>1488
                     /locus_tag="ACG8KN_002625"
     CDS             101..>1488
                     /locus_tag="ACG8KN_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP9189600.1"
                     /translation="MRLLIPSAKIVPEELHHLGKLPAIIYPINQKIVFDYLYDQYKDV
                     CSAIDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCDEPVIINF
                     ADTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNELKEDDGKKHKLF
                     SGVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNWFDIGHAD
                     KYYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVEYVRPRIF
                     EYSTSYINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCSDFKRYSVSGDN
                     IQKSLKDMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEALLSVSVPRE
                     LFDITQFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYELAKLFHSVD
                     GKYDFIIKDLFT"
BASE COUNT          531 a          185 c          273 g          499 t
ORIGIN      
        1 gttattgtgg cttttcttaa aaggacgcaa ataatgaaaa tccttaaaaa tataaaaaat
       61 taacaagcaa atcttgggaa tggaacggag gcacggtgaa atgagattat tgattccatc
      121 tgctaagatt gtaccggaag aacttcacca tcttggaaaa cttcctgcaa ttatctatcc
      181 gattaatcaa aaaattgtat ttgattattt atatgatcaa tataaagatg tatgttctgc
      241 tatcgatatt gcctgttacg aaaagatgga taaggtagca agacgattag ataagtatat
      301 taaatctaaa acagtaaata ttattcagct taaagagtta ggggatcttg gaagaactat
      361 atatgattct ctaataggat gtgacgagcc tgttattatt aattttgctg ataccattat
      421 caatgataat atatattcct tggaatgtga cagctttttc tatgctgaag actattatag
      481 taatacttgg acattctttg aagaaaaaga cggagatatt atttctgttt tagataagaa
      541 cgagttaaaa gaagatgatg gaaagaagca caaactgttt tctggtgtat ttcaaattat
      601 ggatgcccag tattttagag agtgtttgcg taaagctttg atgtcaaatg tagtaaatgt
      661 gaatagtttt tatcaagctt tacaagagta tagcaaacga tatgaatttc tctctattaa
      721 aacaaataat tggtttgaca ttggacatgc agataaatat tataattcca aattggaagt
      781 taaggcaaga gaatttaacc acatttcaat tgataaagat cgtagcatcc tgagaaaaat
      841 tagtgaagat gttgaaaaat ttatcggaga aattaagtgg tatcttaagt tgccagcaca
      901 ggtagaatat gtgagaccta gaatattcga atattctact tcgtatatta atccatatgt
      961 aagtatggaa tactattcgt atcatactgt tcacgagtta tttctctata gtgacttaac
     1021 aaaaaaacaa tggatagata tctttaatag aatacgattt gtatgctcag actttaagag
     1081 atactctgtt tcaggggata atattcaaaa gtcattgaaa gatatgtatc tcgataaaac
     1141 atttcagaga ttcaataagc tcagaaagga cccccggttc acagaattct tttcttctga
     1201 tattcaaata aatggagtca gatataaatc tttggaccag atagaagctt tattgagcgt
     1261 aagtgttcca agagaattgt ttgacattac gcaatttaat attattcatg gtgacttatg
     1321 ctttgccaat attatggtag ataacacatt ttcgttcatt aaagtcatag atccgcgagg
     1381 taagtttgga gattttgata tttatggtga ttatagatat gaactggcaa aattgtttca
     1441 tagtgttgat ggaaaatatg actttataat taaggacttg tttacaat
//
LOCUS       DAZKCZ010000213         1478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000213 DAZKCZ010000000
VERSION     DAZKCZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.218"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1207
                     /locus_tag="ACG8KN_002626"
     CDS             17..1207
                     /locus_tag="ACG8KN_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFP9189601.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1341..>1478
                     /locus_tag="ACG8KN_002627"
     CDS             1341..>1478
                     /locus_tag="ACG8KN_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9189602.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     IL"
BASE COUNT          468 a          289 c          309 g          412 t
ORIGIN      
        1 aagagaggac ttccctatga acgattttac tacagaaatt ctaaagactc tagcgaacaa
       61 aggcgatttg aatgaattat tccgtgtcca tttggagaaa gcagtcaata cgcttctcaa
      121 aacggagtta acggctttcc tagattacga aaagtatgat cgcattggtt ttaacacggg
      181 taattctcgt aacggctcct atgaccgtac ggtcaagacc gagtatgggg aacttcatct
      241 ccagattccg cgcgaccgca atggtgagtt caagcaacag actgttcctg cttatagacg
      301 gacgaatgac acgttagagg agaccgtcat tcacctcttc cgaaaaggta ttaccatgtc
      361 ggaaatcgca gacttgattg agaaaatgta tgggcattac tacacgcccc aaaccatgtc
      421 caatataaca aaatcattta cagaagaggt aacggcgttt aatgggcggg agcttcatga
      481 ccgttatgct gctatttata tggacgcaac gtatattccg ttaaagcgga aaaccgtcgc
      541 caaggaagct attcatatcg cagttggcat tcgcccggac ggatcaaagg aagtattgag
      601 ctatgcgatt gcaccgactg aatccatcac gatttgggag gaaattttat tggaccttca
      661 agagcgcggt ttgaaaaatg tcctcctgtt catcacggat ggcttaaagg ggatggtagg
      721 agcgattagt cggttctatc ccaaagctcg ttttcaacat tgttgtgtac acgtttcccg
      781 taatatcagt cataaagtgc gtgtcgatga tcgtaaggaa gtctgtgatg attttaaaat
      841 ggtgtatcaa gcctcatcta aagaggtggc gttggaagca cgtggtactt ttgcggaaaa
      901 atggaaaacc agctatccaa aagtggttga atcgattctt tcgaacgatc acttgctcac
      961 tttctatgat ttccccttgg ccatacgcaa gagtatttat tctacgaact tgattgaatc
     1021 ctttaataaa caaatcaaga aatacagcca ccgcaaggaa cagttccaaa atgaagagtc
     1081 gatggaacgt ttcttagtct cgtcttttga tacttacaac caaaaattcc taggtcgcag
     1141 tcataaaggt ttccaacagg ccgaaggcga gctcgaacaa atgctaagcc aactgattga
     1201 gaattagaaa caatctgcag tagggaagaa tcatttacac aaaattattg acgctccctt
     1261 ataatgagaa cttttactaa cataatttat gataaactta ctatataatc atattaatta
     1321 aatagttagg agatctgctt atgtttatat ctactcttca aattcgaaat tttagaaatt
     1381 atgaatccga aatctttcga ttcgataatg agacaaatac tattataggt gagaatgatt
     1441 cagggaaaac caatgctctt actgcattga gaatctta
//
LOCUS       DAZKCZ010000214         1473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000214 DAZKCZ010000000
VERSION     DAZKCZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.219"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..266
                     /locus_tag="ACG8KN_002628"
                     /pseudo
     CDS             42..266
                     /locus_tag="ACG8KN_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            526..780
                     /locus_tag="ACG8KN_002629"
     CDS             526..780
                     /locus_tag="ACG8KN_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9189603.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            781..1098
                     /locus_tag="ACG8KN_002630"
     CDS             781..1098
                     /locus_tag="ACG8KN_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9189604.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTIHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQIK
                     LGF"
BASE COUNT          515 a          226 c          287 g          445 t
ORIGIN      
        1 attaggcaga agtcactcat cctctatagg tttgtgatga aattgctatt gatccattta
       61 agtaagagca aaaaactgga ggtttatcag gtaattggtc taatggattt aagtatgcgg
      121 tcaccgtgta tagagttgct tatgagattc aagaaaataa agttattcca gtattgctat
      181 atggaacaca taacaatttt tatgaattag ccgagaacac tcgcaacttt attcgtgtag
      241 atgaatcggc ttcggttgag ttataacttt agctatatca atcaaccgac cgacatatgt
      301 taaatctata tataataaaa atacctactt aaatggatgt taacggaagt gtatggtgag
      361 tagttcactc gtattttgat atcgtttgta tatgttttca gcgttctaag tggcttattt
      421 tgtccaataa taatatggag tggaatccct taagaaacac cgttggcaat tgttgttata
      481 acatgttata atttattcat caaaaactaa aaggagtgtg ataacatggg aaaagaacta
      541 aaggttagaa agatcggcaa tagcgtggga gtgatccttc caagctctct tggattaaaa
      601 agcggagaca ctatccaagc caaacaagaa ggcaacttat tcatattaga tactactcaa
      661 atagcaaaag aacacgatcg caaattgatt gaagaaagtt tccaagactt tgagaaaggt
      721 ttaacagttt cagaaattga aatggtaaaa gctttcggaa aatacggctg gagtgaatag
      781 atgaaagagt acacgctagt ttattctaaa agtttccaga ccagccttga aacgataata
      841 gaagaatggg aaaatgagct tgctctttct ccagccagca taaaaagatt tgtaggtgcg
      901 atccagaagg ctttagaact aaccaaaaat tttccaacta tacatgagga agtttccgaa
      961 atctatggaa tggatatccc aacatatcgg atcgtgatcg gcaaaagcta tgccttattt
     1021 tatcggatcg accatgaaaa aaaagagata cttgtaggac gtattttcca atctaaacaa
     1081 ataaaactag gcttttaaca ataattaggc aaaagtcgct catcctctac aggtgggtga
     1141 ttaatgccgc tcgatatggt tgttaaccgt cggttactcg aagattgata tataagactt
     1201 agtacctttt tattttgctc catgttatgg ccgaaggtct aaagatacaa tcattggtct
     1261 agagaaaaat taagaaccgt aggaactacg gggatcgctt ggtgaataag agacactgct
     1321 attagtgaaa aaataagata tcaagtacgc tctattccca aaaatttcgt gactttactc
     1381 atgagaggtt caagtagtat atttttaatc aaaattgacg taacttttaa taaatactac
     1441 ctaaataagg tagtatttat ttttgtgttt taa
//
LOCUS       DAZKCZ010000215         1471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000215 DAZKCZ010000000
VERSION     DAZKCZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.220"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            360..902
                     /locus_tag="ACG8KN_002631"
     CDS             360..902
                     /locus_tag="ACG8KN_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189605.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1058..>1471
                     /locus_tag="ACG8KN_002632"
     CDS             1058..>1471
                     /locus_tag="ACG8KN_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFP9189606.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMA"
BASE COUNT          569 a          178 c          287 g          437 t
ORIGIN      
        1 ctgcgcgagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt gacatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcaa attgatgaag cagatcccaa
      181 agggctgctt catcaatttt ttcgtaagtg tccgtacact tactgtgctt gctcatatat
      241 taatcgatca tatactaatc gtgtaagaac aactttaagc ataatactta tattgttata
      301 ccatagtttg gaaaaataaa aaattatatt tcatcaacgt ttcataaaag gtgtgtagaa
      361 tgttattgaa agtagctata aaatgctatt gtgtaaaaaa taaaggagga ataatttatg
      421 aaaaaatagt accaagagga gcatgcctat tacttgcatt atgtttgagc ttaagcgcag
      481 ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga aggacaatcc ataagttctg
      541 aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga aacttttaag ccatcagatt
      601 atacattgga ggcaaagaaa gaatatgttt atgaatattt agggcttaaa tttaaactct
      661 cagacaaatt tagaaattat atagctgata aaaagatagc gatgttagat gatcaaagtc
      721 ctatagataa agaactaaag tatgctattt tgacatttga gaaaatgaca gaagaacaaa
      781 agaatgcagt tatagaaaag atgggcgatg gatataaaaa ttggcaaaat gagcttgaaa
      841 gaattgggac tattggtata tttgaaaaaa atacatcaga agaaaaaaat ctaaaattat
      901 aaaatgtgat actcatacta aaataggagt ttcaagtgat ggaaaaaacg attgctattt
      961 aagcacaaat agcggagctg aaagtaatct tctggatgaa ttaaaaagaa ctgagattca
     1021 aattatagat aaaaaagaac gtcctaaaaa tggttttgtg ctatcagaaa aaactgattt
     1081 agaaaataca gaagcattta acaaagaatc ggttaaagat ttacgtaaac taccaacaaa
     1141 ggacataaat ggcaaggatt ttacaagtaa agattttgag aagtatgatc ttactatggt
     1201 aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa attcctgatt tggttgaggt
     1261 tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga gttgttacag atcctgttga
     1321 tgataacggt gaaaacaagg aagcaattga aaagtcaaag ttaatacatg aaaagacaaa
     1381 ggcttcatat cctttcttaa tgccagatgg aatggcaaca ctttttttga caaaaattat
     1441 aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DAZKCZ010000216         1457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000216 DAZKCZ010000000
VERSION     DAZKCZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.221"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..906
                     /locus_tag="ACG8KN_002633"
     CDS             40..906
                     /locus_tag="ACG8KN_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9189607.1"
                     /translation="MDMNIQKYIAFLKTVELKSFTKAAEALNYSQSGISRMINDLEDE
                     WGVTLLERNRTGLRLTSDGINIYPYVKNLFDEYESMQRKLEELNGIKFGVIRIGTFSS
                     VATHWLPQLILNFQNKYPNIKFELLLGDYDEIEHWILTGRVDFGFLRLPTLPKLSTQF
                     IHQDDFKVIVPKNHFLEKKDSVSLNDLSGLPFILLDKNGNSNITTLFETMDFSPDINF
                     TTWDDYAIMAMVEKGLGVSILPELILQRVQYDLTILDLDVPLYRKIGIAYKEEKNLSL
                     ASKKFLDLLFFE"
     gene            1246..1374
                     /locus_tag="ACG8KN_002634"
     CDS             1246..1374
                     /locus_tag="ACG8KN_002634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189608.1"
                     /translation="MQNIKKILLVEMKQVERNEAVNCVAGAISEGLASIDFEAARV"
BASE COUNT          550 a          206 c          225 g          476 t
ORIGIN      
        1 ctttagtatg ataaaaccag atattaatga ggtgtaagca tggatatgaa tattcaaaag
       61 tatattgctt ttctaaaaac agttgaattg aaaagtttta ctaaagcagc tgaagcttta
      121 aattattctc aatcaggaat aagtaggatg ataaatgatt tggaggatga atggggtgtg
      181 acattacttg aacggaaccg aacaggtcta cgtctaactt ctgacggaat taacatttat
      241 ccatatgtaa aaaatttatt tgacgaatat gaatctatgc agagaaagct agaagaactg
      301 aacggcatta aatttggcgt aattagaatt gggacctttt caagtgtggc tacgcattgg
      361 ctacctcaac ttatcttaaa tttccaaaat aaatatccaa atataaaatt tgaattatta
      421 cttggagatt atgatgaaat cgaacattgg attcttactg gtagggtaga ttttggattt
      481 ctaagattgc ccacactgcc aaaattaagt acgcaattta tacatcaaga tgatttcaaa
      541 gttatagtac cgaagaacca ctttttagaa aagaaagatt ctgtttcact caatgatctc
      601 tctgggttac cttttattct cttagataaa aatggaaact ccaatatcac tacattattt
      661 gaaacgatgg acttttcacc tgatattaat ttcactactt gggatgatta tgcaattatg
      721 gccatggttg aaaaaggatt aggtgttagt atacttccgg aattaatttt acaacgtgta
      781 caatatgatc taacaatttt agatttagat gttccattat atagaaaaat agggattgca
      841 tacaaagaag aaaaaaattt atctttagct agtaaaaaat ttctagatct tttatttttt
      901 gaataattca tcaaaagtct tagctcaaaa tatatttaca atcataaaat caaatcgttt
      961 tcagactatt ttgataaaat acgagataga gcctaatatc attacctgac cactaccgat
     1021 cataataatt acttataaca agattgagtt tatccgaaac tattccaaaa gaaatttata
     1081 tactaatcac acttcactaa aattatttca ccaatacaaa tattttccta taagcaagtc
     1141 taaaaaaact gctataagac aaaaaaatag cgatttacga tttttttacc acaatcagta
     1201 tcgagtttaa tataatagta ttatcaaaaa gggagtgtga tcaacatgca aaatataaaa
     1261 aaaatactat tagtagaaat gaaacaagtt gaaagaaacg aagctgttaa ctgtgttgca
     1321 ggagctatca gcgaaggact agctagtatt gattttgaag ctgctagggt ctaatgatat
     1381 gtctactaaa aacaaatcca aaggacttat agtatcaact attatttttt catgctttat
     1441 tctattaggt atccata
//
LOCUS       DAZKCZ010000217         1457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000217 DAZKCZ010000000
VERSION     DAZKCZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.222"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..504
                     /locus_tag="ACG8KN_002635"
     CDS             130..504
                     /locus_tag="ACG8KN_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9189609.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(618..1247)
                     /locus_tag="ACG8KN_002636"
     CDS             complement(618..1247)
                     /locus_tag="ACG8KN_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9189610.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1381..1453)
                     /locus_tag="ACG8KN_002637"
     tRNA            complement(1381..1453)
                     /locus_tag="ACG8KN_002637"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1418..1420),aa:Thr,seq:ggt)
BASE COUNT          489 a          222 c          258 g          488 t
ORIGIN      
        1 tacactctga gatgtaagaa atatcataag atatttctta catctttttg ttttgagcag
       61 gactttagat aattttatta atatatgcta gcatgctttt gtaagaaaaa gaaagagggc
      121 gtattagtta tgtatcttaa aattacggaa caagcacaag aaagattaca aaaatatatt
      181 gatgaaggcg caacagttat attggattta gatgatggcg ttggggaata ttctaaaatg
      241 ggtgtttgct ctttagatac tagttttcgt ttacttcttc ttgataaaga acagagaaaa
      301 aatgattata aactccaatt agatagtgat attggggatg tttatataaa agattattca
      361 aaaatgtaca tggatgaaca tatgaccctt tctttagatc cacgtctagg tgtatttact
      421 cttgaaagcc caagtggttc tttagacagt catgttcaat tagtagatct acgaccagaa
      481 aaattctctg gaataaaaca ataactttct ctctattgtc cataaaacta gtctcaatca
      541 agctgtattg aaaaaacaag acaacattct taccatggta aataaaaaga cgacacattt
      601 tgtcgtcttt ttttaaatta actattttcg ataaaagatc gcatagcttg tgccattatt
      661 tctggctttt ctctattcaa ttcatgtttt ccgtttttaa taaagagtag ccggccattc
      721 gttgaaagtt tatataaacg ttttgctgct ggtttattcg catgatcttt ggtaccgcat
      781 aaaatcaatt taggaagtcg tgatgtacga agctgttttt tgcagtctaa tgtttcaagt
      841 gaacgaagta gggtaagggt ttgtttttta gatagaccta atttttcaaa tatcttagaa
      901 ggtacgcagt aaaaataaat attctggaaa gtaagcaata agcgattagg agactcaaat
      961 tgtggagcga ctaaaacaac cttttttaaa tatggagaag gagttgcaag atagtttaaa
     1021 gcaataacag cgcctaaaga taatccacat aaaataaaag gttcctttat ctgattcaat
     1081 tgtacttcga tgtatttggt taagtcgtct aaagtagcag tgtctgtgct gtttaaatga
     1141 ttgaacaatg aaagactttt gcaagaataa ctattgggta gataagagat tacatctttc
     1201 catgattgtt cgtcttggcc tagaccatgc aaaaaaacaa ttttcatatt gctctccttt
     1261 ttatagttat catatagact ttctttgtac tattaaaata taaacattca tttcttacgt
     1321 cataaaataa aaaaacagcg cagacacatt gttaaaccaa catgttgcac tgtttaagga
     1381 agccacttgc cgggcttgaa ccggcgacct cttccttacc atggaagcgc tctaccagct
     1441 gagctaaagc ggcatta
//
LOCUS       DAZKCZ010000218         1428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000218 DAZKCZ010000000
VERSION     DAZKCZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.223"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            193..792
                     /locus_tag="ACG8KN_002638"
     CDS             193..792
                     /locus_tag="ACG8KN_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9189611.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            856..>1428
                     /locus_tag="ACG8KN_002639"
     CDS             856..>1428
                     /locus_tag="ACG8KN_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HFP9189612.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT          497 a          226 c          287 g          418 t
ORIGIN      
        1 caaatcctac cgaaaatatc aaaaatccaa tgtgacacaa tcagattgtg tctcattcga
       61 gggaatcaca caatcccacg tggctctagg cagaattgaa tttttgcttg gggctttttc
      121 cttatattga ttttgaatga gacttattat atgttataat gcaattaagt atcattggag
      181 agggcgaaga aaatgaagac ggtagaacct attcgagaca aaaaaaagat cgatgcgatg
      241 aaggcaatct tggcttcggg aaaatatggc caacgaaatt tggttctttt ttcgattggg
      301 atcaacaccg cctatcgcat ttctgatttg cgtcaattga agctggccga cgtgctggaa
      361 atttcacgcg gtcgcgtggt ggtcaaagat cgattagcga tgaaggaaca aaaaacagct
      421 aaacataatt cggtctatat ttccaacaaa ttgcgaaaaa cgattttgga gtacgttcaa
      481 caggaatttc ctgatcaact ccttcagcaa gattttgacg gttatttgtt ccctagtcga
      541 aaagggacga atgaaccgct caaccgccaa agtctgtggc gaatcattca tgatgctggc
      601 acggcaatcg gattgaaaga cattggccca cattccatgc gaaaaacgtt cggttacttt
      661 ctctataaac aggggaccaa aacagaaatc attcagtcct tgttgaacca ttcttctcag
      721 cgcgaaacat tgcggtatat tggtattaca caagaagaca aagatacagc tgtaaaaagc
      781 ttagatttat aatataaaag ttaagtttaa acatggatag ttttaaatta gtagattgag
      841 tgttgggagg aaagaatgag tacattatat attataggaa acggttttga tttaagtcat
      901 ggtttaaaaa ctagatacac tgatttttca aaatgggttg aaaaaagtca tagtgaaata
      961 ttcgataaaa tagacacaat gataaatgaa aataatgagg tgagaagtac agctgatgat
     1021 aagataggtt ggtttaattt tgaaagtagt ttgcatgagt taattcaaag tccagtcttt
     1081 gaagattatg tagaagagtc taaggattat ctcataggtt atggacatga agattggtca
     1141 gattctgccc atcatgattt tcaaaatatg gtttatgatt atgttgtttc tctagaagaa
     1201 ttacaacaac ttttcaagga atggatacgc aaagaaatac agccgacaat agaacataaa
     1261 attccaaaat accaattttc gtccgatgta ctattcttta cattcaatta tacagatgtt
     1321 ttggaaattc tatacaagca accgaaagaa aacatatatc atattcatgg cagttatgac
     1381 gatataataa tcggccactc cgagaatttt gacaacttta taggagat
//
LOCUS       DAZKCZ010000219         1424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000219 DAZKCZ010000000
VERSION     DAZKCZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.224"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..493)
                     /gene="tnpA"
                     /locus_tag="ACG8KN_002640"
     CDS             complement(38..493)
                     /gene="tnpA"
                     /locus_tag="ACG8KN_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFP9189613.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            736..1424
                     /locus_tag="ACG8KN_002641"
                     /pseudo
     CDS             736..1424
                     /locus_tag="ACG8KN_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          454 a          274 c          262 g          434 t
ORIGIN      
        1 gaccgccaac tgctcatctt cactcactta cttctcttta ttttttaaat ggttctacgt
       61 actctctttt acttataata tatcgaatgc gatcatcagc ttcttgttca cggatgtatt
      121 tttcatttgt cttttgattc aatccgactg tactcacata gtatctcttc gaccaaaagg
      181 atttgtttcc gtatttatat ttgagatttg cgtgctgctc gtggattagt accgcactac
      241 gacctttcag ataccccata aaactggata tacatatttt tggtggtact cgaacaagca
      301 tgtgaatatg atctagcatt gcatgtgctt ctatgatttc cacatctttc atttcgcaca
      361 acttcctaag tatttttcca agatcttttc gtaattgccc atagattact tttcgttgat
      421 attttggtgt aaaaataata tgatacttac aattccaacg agtatatgct aaacttttat
      481 cgtcatttga catgaaaaaa ctcctttcga tctgatgagc ggttggtagc cactcactag
      541 tatatcgaaa ggagttttta tcgctaaaga tacttcttcc acgcgtagaa acgcgcggtt
      601 ttcttgtttc gcacgcttta cgcacaaaac tgactgaagt cattaagaaa ataaaagggt
      661 ctaaaacttt taagttttgt accgaccccc aaaagttaga ccaaaaaatc taacttttgg
      721 aggtcggtat tttttatagc taagtatact tttgaactta aattaaagat cgttcatgat
      781 tatttggatg gtaaaggcgg aagtgattat ctggcaaaga agtattccat taaggttcca
      841 tcacaagtga agcgctggat caatgcctat caagaatttg gtgaagaggg tctagtaaga
      901 aagcgacaaa agaaaaaata ttctgttcaa tttaagctgg atgcgataga gttatatcta
      961 acaagtgagc tgtcctatcg tgaagttgcc aacacactta acatgaacaa ccctaatttg
     1021 atcgctagtt ggatgcatca attccgtgag ggtgggatcg atggactctc aaaaacgaaa
     1081 ggatgtccac ctatcttgtc taagaaatat gaacccaaaa ataagaaaaa atctacaact
     1141 aaagcaacgt ctaaagaacg tgaacgcatc gaggagttgg agaaacgagt ccgttccctt
     1201 caaattgaaa atgcctcttt aaaagaattg aggaagctgc gaaaacagga agcccaacaa
     1261 cgacgaatga agcaatcgca cgaatcatcg caagcctccg aggaccgttt aaattagttg
     1321 agcttctaga aactttaaag ttccctaagg caacctttat gtactggcaa aaacgattgg
     1381 accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaa
//
LOCUS       DAZKCZ010000220         1405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000220 DAZKCZ010000000
VERSION     DAZKCZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.225"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..674
                     /locus_tag="ACG8KN_002642"
     CDS             <1..674
                     /locus_tag="ACG8KN_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP9189614.1"
                     /translation="NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKI
                     IRFGILGIALIACLVSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHEL
                     FGVGIPNNHDARAVEYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALA
                     ISIIDAGFRRDKSVAAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDE
                     FIYIE"
     gene            691..>1405
                     /locus_tag="ACG8KN_002643"
     CDS             691..>1405
                     /locus_tag="ACG8KN_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WavE lipopolysaccharide synthesis family
                     protein"
                     /protein_id="HFP9189615.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLY"
BASE COUNT          481 a          190 c          279 g          455 t
ORIGIN      
        1 caaataaaag tatatggaat tgcattttta gtttactgac atttgttata atctcgcaag
       61 gcagaagtgg tatattatat gtagtaataa cctcggttat tatgctttta atttcgctta
      121 aaccatccaa gattattaga tttggtatat taggaattgc cttaattgca tgtctagtgt
      181 ctgtgaccca aattagagag gtcgttatat cttcgtttgt agagtctcag agcagtgaca
      241 caggtaatat cgcatataga attgctgaac aagattattt tgaaaataaa ttagaaggtc
      301 atgaactatt tggagttggt ataccaaaca atcatgatgc cagagctgta gaatattcag
      361 gcaaaacaat agattttgag tctaaaaaat ataatcccta ttatcttgag gacttaggcg
      421 cttttgaaat tcgttataga tttggtataa ttgcatatgc gttatatata gttgctttag
      481 ctataagtat tatagatgcc ggatttcgaa gagacaaatc ggtcgccgca tttgcgggta
      541 tggcattcat ggtgtttatg gcgcttaatt ttaatttgct agcgtatatt acagcaagac
      601 cgttcggatt tatgatttct ttaattttga tagaaatatc ggcattcaat gatgaattca
      661 tatatattga ataggtaagg gagagcacag atgaatttag atcgattaaa gaaaatagca
      721 aatatgattt caccaatcat aaaagtgata aatgcgccgt tagcgtattt tagtgggaaa
      781 tcagattata tggagaaggg aatattgtgg cctactgtaa caacctactc caataacagt
      841 agaaaagtga ttcaatatac aactttacct aaaaacgtag acaacatggc tcaaatgcaa
      901 tgggctgggg aaagttttga ggacatatgt atagttcttc aaggtccagt attagaagac
      961 ggtgatttta cactaaatac cgttaaaatg tataataaat actatcctaa agtaaaactt
     1021 gtagtgtcta cttggatagg attatctgaa gagacagaat gcaagttgcg tgaagctggt
     1081 gcagatgttg tattatgcaa gaaacctgaa aaaacaggtg cgtacaatat taatctacaa
     1141 cttacttctg catacgaagg cgtaaaaatg gcgcaaaaac tggggtgtag ttatgcgctg
     1201 aaaacgcgaa ctgatcaaag actatatgca aatgatgtac taaagtattt ccaaacatta
     1261 ttgaatattt ttccatcttc ggactgtaaa gtagtgaatc agcgattaat atttacgact
     1321 tatggaaaat catttagata tcttccgttt aatttatgtg attttttggt atacggagaa
     1381 actagtgaat tattaaaatt atatg
//
LOCUS       DAZKCZ010000221         1348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000221 DAZKCZ010000000
VERSION     DAZKCZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.227"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..155
                     /locus_tag="ACG8KN_002644"
     CDS             <1..155
                     /locus_tag="ACG8KN_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9189616.1"
                     /translation="LELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCA
                     NASEAV"
     gene            282..575
                     /locus_tag="ACG8KN_002645"
                     /pseudo
     CDS             282..575
                     /locus_tag="ACG8KN_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(890..1285)
                     /gene="panD"
                     /locus_tag="ACG8KN_002646"
     CDS             complement(890..1285)
                     /gene="panD"
                     /locus_tag="ACG8KN_002646"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP9189617.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT          404 a          266 c          218 g          460 t
ORIGIN      
        1 atttggagtt atttgactat gtcaattggt ggaaccacct tcggttgcac ggtacacttg
       61 gctacgagac accggttggt taccgtaacc agagattggc gcagcgaatc cttgataatg
      121 agctcggatg tgctaacgct agcgaggcag tctaacttta actgttagct cctgccgaag
      181 atcgtcacat ccgaggaggc tcattgtcaa ggacaatcgg aatagcctac ggaaagaagt
      241 tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt ccaactcttc catacggatc
      301 gtggaaaaga atttgataat gaattgattg atcttatctt agaaacattt ggcattcaac
      361 gctccttgag taagcctgga tgtccatatg attgccgttt cagaagcgac ttacaaagct
      421 tttaagacgg aatttgtgag tcaatatcaa tttgaaacga tggcacactt agaattagaa
      481 tgttttgatt atgtcaattg gtggaatcat catcggatcc atagttctct tggttataaa
      541 acaccaattg aggtactatg cggaggttgg cgtagcgaac ccttgataat gagcaagaat
      601 cggttacctt ctgttcgagc ggcttatttt attcaaagtg agcacgccgc taagaaaacc
      661 gatttgagga ggctcgttgt caagggaaac tggatatggt gtcataaaca gaccttataa
      721 tttttgtcta gaaaagtgtt gccattccat gtagacatca tccataaaat ggactttgtc
      781 tacagtctgt atcttctctt tttgtgtcta ctttattgac atctatccaa ttagtaatca
      841 ataaatattt caataatagt ctagctatat ataaataaca ttacaaaacc taaagaatta
      901 tatctgataa ccttccatgt ttttcatatg atgttactct ttcaacttta ttattatcat
      961 cgacaaaaac aaccttaggt ctatgatcct taacctcatt ttcatttaat tcacaatatg
     1021 ccattaaaat tattttatct ccagttgata cacatctagc tgcagctcca ttcaagcaaa
     1081 tcatccctgt tcctctctct ccagctattg tataagtttc aaatctattt ccattattaa
     1141 catcaactat ttgtactttt tcatattcat atattcctgc tgcttctaat aaattcatat
     1201 caacagtaat acttcctaca taatctaact cagcttgttc aactgtagct ctatgtattt
     1261 ttccttttaa cattgtaact ttcattatag ccccctattg taattcaaat gtaaaattat
     1321 ctattaatct tgtttttcct ataaatac
//
LOCUS       DAZKCZ010000222         1320 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000222 DAZKCZ010000000
VERSION     DAZKCZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.228"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..>1320
                     /locus_tag="ACG8KN_002647"
     CDS             40..>1320
                     /locus_tag="ACG8KN_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFP9189618.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          448 a          258 c          252 g          362 t
ORIGIN      
        1 atcaccacaa aataaacaac tgagaggacg atacgctata tgaatgattc tatcaaaaaa
       61 atgctgagaa taacagagaa agatttgatg attacagagg tctcttacga gacccttcag
      121 aagaaaaaga cgttggtcgt cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt
      181 tgtggttcta ctgtggtaga tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag
      241 gaaacgattg tccgttttga acaatacaat catatgcctt tggttatgcg cctaaaaaag
      301 caacgctata cctgtaaaaa ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa
      361 cccagacatt caatcgcaaa tcatgttaga tataaaattg cttctttact gactgaaaaa
      421 gtatctttat cttttattgc gaaaagctgt caggtatctt tgaccaccgt tattcgtaca
      481 ttgaaagagt ttaaaagcta tttaccaaag caatctaaga agatcctccc cagagtattg
      541 atggttgacg aatttcgttc gcatgcttcc atagaagata agatgagctt tatttgcgcg
      601 gatggcgaaa caggaaaatt aatagatgtt ttgcctacgc gtaaattacc tcgattaaca
      661 agctatttct taggttgtac caatccagaa gaagtagaat tcttggtgac agacatgaac
      721 gccgcctact tccagctcac caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg
      781 tttcatattg tcaaacacat gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat
      841 gaacttcgta aagccggaca gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt
      901 ttgctaaaaa atcgtgcaaa catcaaccat tatgaataca aaacatggaa aagtttccga
      961 gcaccaaaat acccatttct tactgaagca atgatgattg atcgattgct tgagttttct
     1021 ccgcccctaa aggaggcgta tccctttttt catgaattag ttgaagcctt tcgagacaaa
     1081 gaccctgact tatttttctc cttattggca gaacttcccg aaacgttaga tgacagcttt
     1141 cgggaaaagc ttcaaaacct tctgacctat gaagaaggca tcaccaacgc aatgatctat
     1201 ccttattcca atggaaaaat agaagcgaag aatacccaca taaagacaat gaaacgagta
     1261 tcctacggat ttaaatcatt tgagaacatg agaattagaa tctttttgat caatcaatta
//
LOCUS       DAZKCZ010000223         1251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000223 DAZKCZ010000000
VERSION     DAZKCZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.230"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1251
                     /locus_tag="ACG8KN_002648"
     CDS             <1..>1251
                     /locus_tag="ACG8KN_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189619.1"
                     /translation="IELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQR
                     SKIARLLTEIVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEE
                     INIRNVLFGVYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTT
                     ISLLRAYGNQPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFL
                     ITEEEFELDGAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMG
                     MMHVNRKNWIFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMI
                     RLFYDYLFLLVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRIT
                     SRTNIYLSDCLLEKFNKEQIIWKNP"
BASE COUNT          443 a          181 c          224 g          403 t
ORIGIN      
        1 atagaattaa ataaagaatt tactgaattc aaaacaaagc cagagatcca aatttcagaa
       61 gaagcagaga tccatgtcga acgtctgaat ttgacggata tcaaaaattt aagaagaatc
      121 tactttcaac gatcaaaaat tgctcgactg ttaacggaga tcgtggattc aaaagtaacg
      181 atcgaaaaat ttgcagagaa tcatttttta agcatatcaa gagcctatgt aaaaagaaga
      241 gaattagtct cttttcttga acagcatcaa ataaagttaa agaaaaacat attagttggg
      301 gaagaaataa acatacggaa cgtattattt ggggtatatt tcggggtctt taatggtttt
      361 tctatgccat ttagagaaag cgtcatgaat gaagcaaaga gtatcgtgca atattttgaa
      421 tatctgttca atttaaaact gtctgcaaca aaacgaatca aattcaattt gctaaccact
      481 attagtttgt tgagggccta tgggaatcaa ccaatcggaa aacgtttctt ttcagatgaa
      541 tactttaaga gctcagaagg aatgaagatc atcaaggatt tatcacatat ttcttttctt
      601 catatcccta aagagcagat cattgatgag atttcttata tcatgttgtt tttgatcacg
      661 gaagaagaat ttgaattaga tggcgcattt ggacttgcgg aagctcattt tcatgatatc
      721 cacatgatta gtcaaaaaat gtcagtggaa ttgattgatc aattaccatt ttcgattgaa
      781 atctctgaag aagaaagact atatatacga aaagaacttc gtatgggtat gatgcatgtc
      841 aatcgaaaaa attggatatt cgactttgag atgactagct ttacgtctaa aaagcagcta
      901 cagtttttct tcgagacgta tccaatattc agtacagcaa tccaaaaggt catacaagag
      961 aataaacgct atttcttggg aaaagatgaa cgtttcatga ttcgcttatt ttatgattat
     1021 ctctttttat tggtcggtat ttgtccggta tcatattttg aagcaccgat tcatgtctgt
     1081 attgattttt cgcaaggatc tgcttatacc gattacatta tttctcaagt gaaaggattt
     1141 aaaaattaca atattgtgat tgagagtcga atcacttctc gtacgaatat ttatttatct
     1201 gattgtttac ttgaaaaatt taataaagaa cagataattt ggaaaaatcc a
//
LOCUS       DAZKCZ010000224         1241 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000224 DAZKCZ010000000
VERSION     DAZKCZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.232"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>1241
                     /locus_tag="ACG8KN_002649"
     CDS             22..>1241
                     /locus_tag="ACG8KN_002649"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HFP9189620.1"
                     /translation="MKKNQPNVVLIVVDQMRADALSLNSQDKIISTPTLDMMASQGYN
                     FENCYSPVPSCVPARAALLTGLDQETSGRVGYEDEVPWNFKNTLPEVFKEQGYQTECI
                     GKMHVYPSRKRLGFDHVLLHDGYLHVDRKYDKSYGEQFEYSSDYLMFLKESLGSDADL
                     IDDGLNCNSWEARPWMYPEKFHPTNWVVSEGINFLRRKDPTVPFFLKLSFEKPHAPLN
                     PPKYYFDMYMDRLPDTLDLHIGNWEKLEHVVPDVCALRGKLKEDDQRRMLAGYYGLIS
                     HIDHQINRFLMALKEFRHDKDTIIWFISDHGDQLGEHYLFRKGYPYQGSIRIPSFIYD
                     PGDLISAKKHGIKELVKIQDIFPSLVDLVLGQYVNTDGKSVKQLLFGNCEGWRREIHG
                     EHSLGLDSSQYILT"
BASE COUNT          421 a          164 c          260 g          396 t
ORIGIN      
        1 tgaatgattg gagtttttaa tatgaaaaag aatcaaccca atgttgtttt aattgtagta
       61 gatcagatga gagcagatgc gttatctttg aatagccaag ataaaatcat tagtacgcct
      121 acattagata tgatggctag tcaaggatat aattttgaga attgttattc gcctgttcca
      181 agttgtgttc cagcaagagc agcgttatta acaggattag atcaagaaac ttcgggaaga
      241 gtgggttatg aagatgaggt tccatggaat tttaaaaata ctcttccaga agtttttaaa
      301 gaacagggat atcaaactga gtgtattggg aaaatgcatg tgtatccttc aagaaaacgg
      361 ttgggatttg atcatgtact attgcatgac gggtatcttc atgttgatcg aaaatatgac
      421 aaatcttatg gagagcagtt tgaatattct agtgattact taatgtttct aaaagaatca
      481 ttgggctcag atgcagattt gattgatgat gggttaaatt gtaattcatg ggaagcacga
      541 ccatggatgt atcctgaaaa atttcatcca acaaattggg tggtttcaga aggaataaac
      601 tttttaaggc ggaaagatcc tacagtacca tttttcttaa aattatcatt tgagaaacct
      661 catgctcctt tgaatccacc aaaatattat tttgatatgt atatggatag attaccagac
      721 actctagatt tgcatattgg taattgggag aaattagaac atgttgttcc agatgtttgt
      781 gccttaagag gaaaattaaa agaggatgat caaagaagaa tgcttgctgg atactatggc
      841 cttatttctc atatagatca tcagatcaat cgctttttaa tggctttaaa agaatttaga
      901 catgataaag atacaatcat ttggttcatt tcagatcatg gagatcaatt aggagaacat
      961 tacttattca gaaaagggta tccttaccaa gggagtattc gtatcccgtc gtttatttac
     1021 gatcctggtg atctgatctc agcaaaaaaa catggaatta aagagcttgt aaaaatacag
     1081 gatatatttc cttcattagt tgatttagta ttaggacaat atgtaaatac agatggaaaa
     1141 agtgttaaac agttgctctt tggaaactgt gaaggatggc ggcgagaaat tcatggagaa
     1201 catagtttag ggctggattc tagtcaatat atattaacgg a
//
LOCUS       DAZKCZ010000225         1234 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000225 DAZKCZ010000000
VERSION     DAZKCZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.233"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1234
                     /locus_tag="ACG8KN_002650"
     rRNA            <1..>1234
                     /locus_tag="ACG8KN_002650"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          371 a          243 c          363 g          257 t
ORIGIN      
        1 ctttaatagg atgttacgat tgtgtgaata catagcacat tcgaggtaga cgcagagaac
       61 tgaaacatct aagtacctgc aggaagagaa agaaaattcg attccctgag tagcggcgag
      121 cgaaacggga aaagcccaaa ccagcaagct tgcttgttgg ggttgtagga ctccaatatg
      181 gtagttcttt cagatagtcg aatgacttgg aaaagtcagt caaagagggt aaaaaccccg
      241 tagacgaaat gtggaagaca cctaggagga tcctgagtac ggcggaacac gagaaattcc
      301 gtcggaatcc gggaggacca tctcccaagg ctaaatactc cctagtgacc gatagtgaac
      361 cagtaccgtg agggaaaggt gaaaagcacc ccggaagggg agtgaaatag aacctgaaac
      421 cgtgtgccta caacaagtca aagcccgtta atgggtgatg gcgtgccttt tgtagaatga
      481 accggcgagt tacgattgca tgcgaggtta agttgaagag acggagccgc agcgaaagcg
      541 agtctgaata gggcgtttga gtatgtagtc gtagacccga aaccatgtga tctacccatg
      601 tccaggttga aggtgcggta aaacgcactg gaggaccgaa cccacgtacg ttgaaaagtg
      661 cggggatgag gtgtgggtag cggagaaatt ccaaacgaac ttggagatag ctggttctct
      721 ccgaaatagc tttagggcta gcctcggaat tgagaatgat ggaggtagag cactgtttgg
      781 actaggggcc catctcgggt taccgaattc agataaactc cgaatgccat tcattcatat
      841 ccgggagtca gactgtgagt gataagatcc atagtcgaaa gggaaacagc ccagaccacc
      901 agctaaggtc ccaaaatata tgttaagtgg aaaaggatgt ggggttgcac agacaactag
      961 gatgttggct tagaagcagc caccatttaa agagtgcgta atagctcact agtcgagtga
     1021 ccctgcgccg aaaatgtacc ggggctaaac atattaccga agctgtggag tacaccttta
     1081 ggtgtattgg taggagagcg ttctaagggc gttgaaggca gatcgtgagg actgctggag
     1141 cgcttagaag tgagaatgcc ggtatgagta gcgaaagaca ggtgagaatc ctgtccaccg
     1201 aatgactaag gtttcctggg gaaggctcgt ccgc
//
LOCUS       DAZKCZ010000226         1226 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000226 DAZKCZ010000000
VERSION     DAZKCZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.234"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            338..>1226
                     /locus_tag="ACG8KN_002651"
     CDS             338..>1226
                     /locus_tag="ACG8KN_002651"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9189621.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSD"
BASE COUNT          481 a          188 c          246 g          311 t
ORIGIN      
        1 caaaaaaatg tctggaaaat cgaacataaa agtgtctgat ttgaatatcc cagcagcgga
       61 agaagaaaaa caaaatcgat aacatttgtt ctaagaatta gttacgttaa atacggaacg
      121 caagatagac ttgaaaaacg gtaacaaaag aagtagtata ttaaggtgtg atgtgctact
      181 tgacatttat tctgctgagc gaactttctg gattgtgtag ccttatatct attcaggacg
      241 tatcgagcag attagtaaag gtgttcttga tgattttcat cttgaacacc tttgttttta
      301 gcaattcgca aaaaacgaaa agtaggagaa ttgataaatg aaaaaaagat ttttagcact
      361 tgccatcgta ctaggcactg gcttattgtc aggctgtacg aatgccggtg aaaaaacagc
      421 cgttagttat aaaggcggta caatcagcga acaagaagta atggatagct tgaaaaaaat
      481 gcaaggagca gattccgctg tgcaacagtt gatcgtttat caagtatttg aagataaata
      541 tggagatgac gtatcaacca aagaaatcga ttctcaatat gatcaaacaa agaaacaatt
      601 gggtgattca tttgattcac aattgaaatc tgcaggttac acagaacaaa cattcaaaga
      661 cagtatcaaa caaagtctag cctttcaaga aggtctaaag aaacatatca aattgaccga
      721 tgaagattta aaaacggctt gggaatcttt ccatccagaa gtggaagctc aaattatcca
      781 agtagcaagt gaagatgatg caaaagatgt gaagaaagct gctgataagg gagacgattt
      841 ctctaaatta gccaaagaca agtcaactga tactacgaca aaagaagatg gcggaaaagt
      901 gaaatttgat tccactacta caacggttcc ggcagaagtc aaagaagcag cctttaagtt
      961 gaaagatggt caagtttctg atgtgatcac ttctaccaat gcttcaactt atactacgga
     1021 atattatgtg gttaaaatgg tgaaaaacca aaacaaaggc aacgacatgg ataaatacaa
     1081 aaaagaattg aaagaaattg caacagacac aaaattaagt gacagtacct tccagaataa
     1141 agtaattggc gaagtattga aagacgctaa tgtaaaaatc aaagacaaag actttgaaaa
     1201 tgttttgagt acattcactt cagatt
//
LOCUS       DAZKCZ010000227         1225 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000227 DAZKCZ010000000
VERSION     DAZKCZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.235"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..997
                     /locus_tag="ACG8KN_002652"
     CDS             44..997
                     /locus_tag="ACG8KN_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9189622.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            969..>1225
                     /gene="prgO"
                     /locus_tag="ACG8KN_002653"
     CDS             969..>1225
                     /gene="prgO"
                     /locus_tag="ACG8KN_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFP9189623.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIY"
BASE COUNT          441 a          188 c          234 g          362 t
ORIGIN      
        1 gaagtatact aaatatattt agtataggaa attggaggga actatggaaa acttacggac
       61 tgcatttaaa acatcagata aactaggaaa agttgtaatg cttacaggta gaaaaggcgg
      121 cattggtaag actacagaca atgatttatt agcgattgtt tctagtcaac tttttgaaaa
      181 agacgtgttg ctaattgatt atgaccaaca aagaaatact acttccaaca ttggatcaac
      241 gtatcaaatt actagctttg atcgatccat gtctgcagca atcaaaaaag gggactgggt
      301 ttcaggtatt actcaagtaa gtccacattt atatattatg gctggctctc ctggatctga
      361 agaacttaat gaatacttgt ccgaaaaata tcctgatcgt cgtaagagat cattggcctt
      421 cattaaacct ttagaagaat tgagaaaaaa tttcgattat atctttatcg actgcccacc
      481 atcaacagat aatgtggttc gtgcattctt aactgctgca gattatatta ttgcaatgca
      541 agaattaaaa cggtacgcaa tggaaggaac agaagatttt attaataaag ttttagttcc
      601 gattgtcacg aattttgaag agtctcatct tcaaatcatt ggaattttgc ctgttctttt
      661 ctcagtgcga cgttcctctc aacacgcaaa ctatcaaaaa acaattgata agtatggtga
      721 atcaaacatt tttaaaagca ttgttaaagg ctcggatcgg ttagaaatgt atggtgagaa
      781 tggtatccaa ttaaatgatt atgtagatcg aagatggtgg gctatttttg cagacatctt
      841 tacagaatta gaagaaagaa ttgaatacta tgaacaacat ggtgatattg aaggatttga
      901 ttatacacct aaatatgctg actcaatggc caatcatatc ctacctcttg gaaaggagat
      961 taaactaaat ggcattatta ccaacgaaca aagataagaa gaaaattggt gctgaagata
     1021 tttcaattaa agatgatatt aatgtcgagt taggggaacg aaagtacact gcgaaagaaa
     1081 gaaaaccagt tcaagtagat cctcccgtac taaaaaccat tcgggatctt agttatgcaa
     1141 aagatattcc tatgtatgaa atagtcaaac ttgcaatgga cgcctatgtt gatactttaa
     1201 acgacgagga acggattata tataa
//
LOCUS       DAZKCZ010000228         1192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000228 DAZKCZ010000000
VERSION     DAZKCZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.236"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..536)
                     /locus_tag="ACG8KN_002654"
     CDS             complement(<1..536)
                     /locus_tag="ACG8KN_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HFP9189624.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPN"
     gene            662..1072
                     /gene="tagD"
                     /locus_tag="ACG8KN_002655"
     CDS             662..1072
                     /gene="tagD"
                     /locus_tag="ACG8KN_002655"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFP9189625.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
BASE COUNT          469 a          190 c          159 g          374 t
ORIGIN      
        1 aaatttggat ctgtatatac accaaataaa cggttgccta accaccctaa acgaactggg
       61 taatacttat attttattgg aatttttatg ccatattgaa tgacaaacca gtataatgat
      121 actaaattag ctaaaaacca taccaatgaa agtatccagg taaatacttt gaaaaaaaag
      181 ttactactat agtctttgcc aaactgataa atcataaaga aaaagattaa ttgccacatg
      241 attaacttaa tattttcgct atatccatat tttaaattca ttaaactgct tactagcatc
      301 gcgccaataa aagcaattaa tagccaatca tattttttta gatttttttc tctaaatgca
      361 ataattgcat ctataatgat caagacaact cccgctattc cagcaggcca aaaaagtaaa
      421 gtatcatatc tagtaggtaa tagtaaatac gggcctattg ctcgtaacat taaaataaaa
      481 ccaaaataaa taaaatacca acctttcaca tattttaaaa atctttcatt caacattttt
      541 cctccatcca cctatttctc ataaatattt cttatccttt ttaggataaa ctggtccttt
      601 caattctaac aaaaaaatgc taaactaaca atgtatatta atgaatttcg agaggtgaaa
      661 gatgaaaaga gtaattactt acggtacctt tgatttgctt cattacgggc atatcaattt
      721 actgaaacga gcaaaacaat acggagacta tcttattgtt gccctttcta cagatgaatt
      781 caattggaat gcgaaacaaa aaaaatgtta ttttagttat gaaaaaagaa aacagttact
      841 tgaagctatt cgctacgttg atttagttat tcctgaagaa aattgggaac aaaaagtaaa
      901 agatataaaa gaataccatg cagattattt cgtgatgggc gatgattggg ctggacattt
      961 tgatgaactt gaacaactaa cagatgcaca agtgatttat ttaccaagaa cacctgagat
     1021 ctctacaact caaataaaga acgaacttaa ttctttaaat agtgataaat aatatttttt
     1081 aaacaataaa agaacaatat atctggataa gacgtaaaaa aatggctcta taatttctag
     1141 gactgataga attttctgtc ggtcctagat tttttgtaaa aaaaaacaga ca
//
LOCUS       DAZKCZ010000229         1190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000229 DAZKCZ010000000
VERSION     DAZKCZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.237"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1102
                     /gene="xerS"
                     /locus_tag="ACG8KN_002656"
     CDS             17..1102
                     /gene="xerS"
                     /locus_tag="ACG8KN_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFP9189626.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          480 a          196 c          185 g          329 t
ORIGIN      
        1 cgaaaggatg aataacatga aaaatacaga aatatatcaa aaaatagata caatcatact
       61 tcatatgccc gattatataa aagattttgt tcaagatcgt gaggataaag atcaatcacc
      121 tcgaactact ttagaatatc taaaaaatta taagctcttt tatgaatggc ttctgagtga
      181 gtctatcgtt cccgatactt caatcacctc gataagacag ttaactgcct atgatctaaa
      241 cttgtacaaa tctcatttga aacgtcgcac aaaggaaaat gtaaaaaaag agacaacaaa
      301 aactaagctc aaagataata ataacttagg tctatctact tctactatca atagaaatat
      361 cacagcatta aaggttctat ttaaatatct ttcgaaaagt tccaataatc ctttaggaaa
      421 accatactta gaaaataatc ctatggatca agtagcaact atcacagata aaactacgtt
      481 agctgcccgt gcaaacgcaa tcgagaaaaa attatttcta gacgaagata cacaaaatta
      541 tctagattat attgccaatg aatataaaaa aactttgagc aaacgagctt tgatctatta
      601 tcaacgagat gtagaaagag acctggcgat caatgcattg attttaggat ctggattaag
      661 actctccgag gttgtaaata ttaatttaga ggacctatct ttagaaaaga ataatgtggt
      721 agtaactaga aaaggaaata agagagacgc agtaaatata gcagcatttg caatggaata
      781 tttagctaat tatctagcta ttcggaaaga acgatacaaa gtaacagaaa acgaaaaagc
      841 tttatttcta gcaatttatc aaggagaagc caagcgtata tccggtattg caatcgaacg
      901 gatggtagca aaatattcaa aaggattcag agtccaagtc agtccacata aattacgtca
      961 tactttggct actagactct atcaacagac aaattcattg gtattaactg cccaacaatt
     1021 gggacataca agtacaaata caacaacttt atatactcat atagacaatg cagctacaat
     1081 tgatgctttg aacgctttgt aaatgtagta aaaaatagta taataatgaa gcctgtctct
     1141 aaaattagat aaaagcccaa ctttaaaggc aagcttcatt atcattttta
//
LOCUS       DAZKCZ010000230         1186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000230 DAZKCZ010000000
VERSION     DAZKCZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.238"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..1180
                     /locus_tag="ACG8KN_002657"
     CDS             140..1180
                     /locus_tag="ACG8KN_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9189627.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          478 a          171 c          197 g          340 t
ORIGIN      
        1 tcattttatt tctgatttta ggtagtattt aattaaaaaa cagttttaat gcgtttgcat
       61 tttttattta tctttgctat tttcgaaaag agtaggtaat tttacataaa taaaacaacg
      121 tgaaagtaga ggatctacaa tgactgattt taaatttttt aaggcggata gagtttacaa
      181 cgaattattt tatcaatttc caaaagtctt tattgtttct gacgaataca aaaaaatgaa
      241 agattcaact aagattgcct atatgctttt aaaagcaaga ttagagatcg caatcagcaa
      301 acggcaaatc gatgaagaag gtaatgttta ttttacttat acgacaaatg aactatgtag
      361 agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa aaagagttgg aatcctttgg
      421 tttattatta caaaagcaga tgggatttaa caaacagtta gggaaaaata atcctaatag
      481 actatatcta gcagaattaa aagtctcaga aaatgatatc tacttactcg aaaaatttga
      541 tagagagaat agggaaaacg ttgataaatc agagggtatg aaaatcatac ccaccctcga
      601 cgaaaaatca gacgctgaat cccttggggc tcaagagggt atgaaaatca taccgtgcca
      661 aaacgttgat aaatcagagg gtatgaaaat cataccagaa cttaataata atatattaga
      721 cactaataga cacaatatag acactgaaaa agaccgccta caagatcaat tgttgttaga
      781 caattttgag acaattatga caaacgacag cattgctacg tttgtccctg aacgagtatt
      841 aaatttgata aaaacatttt cttcaagtta cagtgaagct caaaaaaccg tccagactat
      901 tcataatgca aagaaaaaag ctgaaataga aagtggtatt tcgatagttt ttgaagaact
      961 cgatagttat tatgtcaatg cagaacaaga attatacacg acactgttaa aagcctatca
     1021 aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg atttttgtct atgtaaaaaa
     1081 ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt gaaaaacgtt tgaattatga
     1141 aagctcccca agcactatta cgaaagactg gttagagtga aacttt
//
LOCUS       DAZKCZ010000231         1087 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000231 DAZKCZ010000000
VERSION     DAZKCZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.240"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..1079
                     /locus_tag="ACG8KN_002658"
     CDS             54..1079
                     /locus_tag="ACG8KN_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HFP9189628.1"
                     /translation="MKTIAVVGGHGQVGKGNVRELTNHGYFVVLMGRNLKKMEVFAQE
                     FLPILEQSEVDLNKFVIPEKLADLDMVIVCLDQTDTQFVEQCQKLGIDYLDISANSDF
                     LKKVNLLPIPEKSKRVIGLGLAPGVTNLAAAAYTKKEPSAQMIVIDVLLGIGDRHGEA
                     AVRWTFEQLNRTYIHPKWAQPIENFVYSRKTDFGPPFGKRRSANFGFADQHLLFSADP
                     SRNYVTFLGLDQNWLTALLRTIKKVGLARLFKNPRVIKSPVWFMKRGLVGDRSFVVKV
                     SNGQVKDPEKIVLFGEEEAKMTAEIAVFVANKILIETLPNGHYHLTELTTMSEILSQL
                     PRLSATE"
BASE COUNT          373 a          165 c          232 g          317 t
ORIGIN      
        1 gatttagcct atcggtaaaa atgaattaac ttggtagata aaggagaaga aagatgaaga
       61 cgatagcagt agttggcggt catggacagg ttggaaaagg taatgttaga gagttaacaa
      121 atcatgggta tttcgttgtt ttaatgggaa gaaatttaaa aaaaatggaa gtatttgccc
      181 aagaattttt acccattcta gaacagagtg aagtagactt gaataaattt gttattcctg
      241 aaaaacttgc tgatcttgat atggtaattg tttgtttgga ccaaacggat acccagtttg
      301 tcgaacaatg tcaaaaatta ggaattgatt atttggatat cagtgccaat agtgattttt
      361 tgaaaaaagt taatttgcta cccattcctg aaaaaagcaa gagagtcatt ggtttagggt
      421 tagcacctgg tgttaccaac ttagccgcgg ccgcgtacac taagaaagag ccatctgctc
      481 aaatgatagt aattgatgtc cttttaggaa taggtgatcg tcacggtgaa gcagcagtaa
      541 gatggacctt tgagcaatta aatcgtacgt atatacatcc taaatgggca caaccaattg
      601 aaaattttgt ctatagtcga aaaacggatt ttggaccacc atttgggaag cgtcgcagtg
      661 ctaactttgg ctttgccgat cagcatttac ttttttctgc agatccttca cgcaattatg
      721 taacatttct cggtttggat caaaattggt tgactgcatt gttaagaaca ataaaaaaag
      781 ttggactagc aagacttttt aaaaatccga gagtaataaa gagccccgta tggttcatga
      841 aacgaggatt agtcggagat cgttcttttg ttgtaaaagt atcaaatgga caagttaaag
      901 atcctgaaaa aattgttctc tttggagaag aagaagcaaa aatgactgca gaaatcgcgg
      961 tatttgttgc gaacaaaatt ttaatagaga cattaccaaa tgggcattac catttaacag
     1021 aactaactac catgtcggag atactaagtc agcttccacg tttatcagcc acagaataaa
     1081 ttgaaaa
//
LOCUS       DAZKCZ010000232         1066 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000232 DAZKCZ010000000
VERSION     DAZKCZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.241"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..843
                     /gene="vanY"
                     /locus_tag="ACG8KN_002659"
     CDS             <1..843
                     /gene="vanY"
                     /locus_tag="ACG8KN_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFP9189629.1"
                     /translation="ALFSQEKVEFQNYDQNPKEHLENSGTSENTQEKTITEEQVYQGN
                     LLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDSNIYMSKEIAQKFSEMVNDAVK
                     GGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEHNSGLSLDVGSSLTKMERAPE
                     GKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIRYVGLPHSAIMKEKNFVLEEYMDY
                     LKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEISGNNIDGVIVTVFPGSTHT
                     NSRR"
BASE COUNT          392 a          150 c          216 g          308 t
ORIGIN      
        1 gcactgtttt ctcaggaaaa agtcgaattt caaaattatg atcaaaatcc caaagaacat
       61 ttagaaaata gtgggacttc tgaaaatacc caagagaaaa caattacaga agaacaggtt
      121 tatcaaggaa atctgctatt aatcaatagt aaatatcctg ttcgccaaga aagtgtgaag
      181 tcagatatcg tgaatttatc taaacatgac gaattaataa atggatacgg gttgcttgat
      241 agtaatattt atatgtcaaa agaaatagca caaaaatttt cagagatggt caatgatgct
      301 gtaaagggtg gcgttagtca ttttattatt aatagtggct atcgagactt tgatgagcaa
      361 agtgtgcttt accaagaaat gggggctgag tatgccttac cagcaggtta tagtgagcat
      421 aattcaggtt tatcactaga tgtaggatca agcttgacga aaatggaacg agcccctgaa
      481 ggaaagtgga tagaagaaaa tgcttggaaa tacgggttca ttttacgtta tccagaggac
      541 aaaacagagt taacaggaat tcaatatgaa ccatggcata ttcgctatgt tggtttacca
      601 catagtgcga ttatgaaaga aaagaatttc gttctcgagg aatatatgga ttacctaaaa
      661 gaagaaaaaa ccatttctgt tagtgtaaat ggggaaaaat atgagatctt ttattatcct
      721 gttactaaaa ataccaccat tcatgtgccg actaatcttc gttatgagat atcaggaaac
      781 aatatagacg gtgtaattgt gacagtgttt cccggatcaa cacatactaa ttcaaggagg
      841 taaggatggc ggaatgaaac caacgaaatt aatgaacagc attattgtac tagcactttt
      901 ggggtaacgt tagcttttta atttaaaacc cacgttaact aggacattgc tatactaatg
      961 atacaactta aacaaaagaa ttagaggaaa ttatattggg aaaaatatta tctagaggat
     1021 tgctagcttt atatttagtg acactaatct ggttagtgtt attcaa
//
LOCUS       DAZKCZ010000233         1059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000233 DAZKCZ010000000
VERSION     DAZKCZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.242"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..350
                     /locus_tag="ACG8KN_002660"
     CDS             <1..350
                     /locus_tag="ACG8KN_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HFP9189630.1"
                     /translation="SINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLS
                     GYTEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITR
                     PQEIASRFVKALS"
     gene            364..>1059
                     /locus_tag="ACG8KN_002661"
     CDS             364..>1059
                     /locus_tag="ACG8KN_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9189631.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSSKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIISSVFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAG"
BASE COUNT          340 a          139 c          224 g          356 t
ORIGIN      
        1 tatctataaa ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg
       61 cagatatttc tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag
      121 gaactacttt aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt
      181 tgattaaaaa attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc
      241 cagaacaagc aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta
      301 ttactcgacc acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat
      361 aaaatgttta aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta
      421 ccagtagccg gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat
      481 gcttatccat ttttagatca accgtggttg catacaattt taagtattat gagttatgct
      541 ggaaatgcag tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt
      601 gcaaatggag ataaaggaac agctggttta gctggtggag tagcttatct agtatatact
      661 gcaactatta gtgggtttct tgaacttttt tcatcaaaag atgcaacact agatacaggt
      721 gtagtgggat caattgttgt tggtggtact gttgcatttt tacacaatcg atatagaaaa
      781 attgaattac cacaattctt aggttttttt ggaggatcaa gatttattcc gattgtttct
      841 agtttagctg caatcattat tagttctgtc ttttacttaa tttggccacc aattcaaaat
      901 ggtttagtag ttgctggtga acaaattgct caaatgggta gtttaggaac ttttttatat
      961 ggttttttat tgcgtttaac aggtgctgtt ggactacacc acaccattta ccctttgttc
     1021 tggtatacat ctttaggtgg taccgaaact gtagctgga
//
LOCUS       DAZKCZ010000234         1029 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000234 DAZKCZ010000000
VERSION     DAZKCZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.243"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..>1029
                     /locus_tag="ACG8KN_002662"
     CDS             56..>1029
                     /locus_tag="ACG8KN_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9189632.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NILFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFL"
BASE COUNT          404 a          126 c          178 g          321 t
ORIGIN      
        1 ctggtaatga tacatttgta aaaataaaaa agatgatttt taggaagtga agagagtgca
       61 taaaattagt gttatagtac ccgcatataa cgtagaaaac tatttagaaa actgcttaga
      121 atctatttcc aatcagacac attcaaatat tgaaataata gtagtcgatg atggttcccc
      181 ggatcgaagt ggggaaattg cagaagaata taaaaagaaa gatatgcgat taaaagtgat
      241 acatcaagtg aatggtggtt taagtgcggc taggaattct ggtcttgatt ctgcaacagg
      301 agattttgtg tgctttattg acagcgatga ttggatagaa ccagactatc ttgagttact
      361 atattttggt acgcaaactt atggagcaga tatctcggtg ctaaaaataa aaaagatttc
      421 aagtttagaa caactaaaag aacatacaat tgctgagaga aaatgggaaa tatttgatag
      481 agaacaagca atgaaaaact tatttataaa taataaaatt ggctattctg caaataatat
      541 attattcaat agaaatttat ttgataatat aagattccca ctgggtaaat taatggaaga
      601 taaggctact acttatttgt tgatcgatca ttccgaaaaa gttgctgtca attcatctgc
      661 taaatatcac tattaccaaa gccctaaaag tatactgaga ggaagcttca atcctagaat
      721 ttttgattct tttgaagtcc atgaagatat actagtattc atagataagt tttatccgga
      781 attatcaatt aatgttagaa ctcgctatgt ctatgaagca ataaggatga tgatgacaat
      841 gattaaaaat aactatacca ataaagaaga ttatttacgt tctataaata ttttacaacg
      901 ctttgaaaaa gaagtgaaaa ttgatcccga cttttcacca aaattaaaaa aattagtaaa
      961 gtttttatta ataaataaaa acttgcctta ctatatgtca agtagtaaga tagcaagtaa
     1021 atttttgat
//
LOCUS       DAZKCZ010000235         1023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000235 DAZKCZ010000000
VERSION     DAZKCZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.244"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..935)
                     /locus_tag="ACG8KN_002663"
     CDS             complement(117..935)
                     /locus_tag="ACG8KN_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9189633.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
BASE COUNT          350 a          175 c          127 g          371 t
ORIGIN      
        1 ttggtcaata agagccaccg ctattagtaa aaaataagct atcaagtatg ctctcatccc
       61 aagaattgcc tacttcaatc gttaaaaacc gttaggttta gctaagtggg ggtagttcac
      121 tcaatacttt tatcaataat agagcgcaaa actttttctc ttaattcaac tgaatcaata
      181 ttattgtttt gttcataacc atgaactaat agatcacaaa tataaagttg tgatattttt
      241 ccagctaaag atccaccatt aagaaattct tcaatggctg tttgtaaaac taagtctgca
      301 gactttccta taggtgaatg aatatagtta gttatagcaa taatttttgc gccatttttt
      361 ttagctattt tcaatgaatc ataagtatct ttcgttttcc cagataaaga aaaaatgatt
      421 actaaatcat taactgttaa taaagaagcg acttgggctt gaaaatgagg gtctagtaca
      481 gcttttgctt gaactcctac gcgaagaaac atattttcta aatcaagact agtatttcca
      541 ctagatccca ctccaaatat atatatgttt tttgattgag tgatcatttt aatggcttca
      601 ttcaattttt cttcgtctaa aagcctaata gtggaataga gagcttctgt taaactatta
      661 gcaatttcat catagtactt tacactagtc ggatgttctt ttttttgact aaagtcttcc
      721 tttgcaattt caatttttaa atcagaaaaa ccagaaaaac caattttttg acaaaagcga
      781 ataatagtag catcgccaac ttttgtgttt ttcttaatat cactcatcgt actataaata
      841 atagttttcc cagaatttaa aataaaatca gcgacttttt tttcagactt cgttaaaaat
      901 ggataatttt ctttaattaa atagatataa ctcatacaaa ctcctctaca atttcaactt
      961 ttctccatta tactccaaaa taataaaaaa aactcctaaa tactccattt ttatctttac
     1021 ttt
//
LOCUS       DAZKCZ010000236         1001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000236 DAZKCZ010000000
VERSION     DAZKCZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.245"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1001)
                     /locus_tag="ACG8KN_002664"
     rRNA            complement(<1..>1001)
                     /locus_tag="ACG8KN_002664"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          210 a          311 c          227 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc g
//
LOCUS       DAZKCZ010000237          977 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000237 DAZKCZ010000000
VERSION     DAZKCZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.246"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..977
                     /locus_tag="ACG8KN_002665"
                     /pseudo
     CDS             153..977
                     /locus_tag="ACG8KN_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          319 a          189 c          191 g          278 t
ORIGIN      
        1 aaaagtgagt ctctttttga gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg
       61 attttccacc aacacaaaat attatagaag caaaatcaat tctaccttag atgttcttat
      121 atcactctat cagtcctatt tgacaaagag ccaagaaaaa gacgttggtc gtcgatgctg
      181 ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg
      241 ggaaagcaat tatagtgaaa aatgggaaga aggaaacgat tgtccgtttt gaacaataca
      301 atcatatgcc tttggttacg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgta
      361 cccattggac ggctcaaagt tattttgtcc gactcagcca ttcaatcgca gatcatgttc
      421 gatataaaat tgcttcttta ctgactgaaa aagtatcttt gtcttttatt gcgaaaagtt
      481 gtcaggtatc tttgactacc gttactcgta cattgaaaga gtttaaaagc tatttaccaa
      541 agccttctaa gaagattctc cccagagtat tgatggttga tgaatttcgt tcgcacgctt
      601 ccatagaaga taaaatgagc tttatttgcg cagatggtga aacaggaaac ttaattgctg
      661 tcttgcctac gcgtaaatta cctcgattaa caagctattt cttaggctgc accaatccag
      721 aagaagtaga attcttagtc acagacatga acgccgctta cttccagctt accaaacgtg
      781 ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tgtcaaacac atgaatcagg
      841 cactcaatga gttgcgtatc cgtgaaatga atgagcttcg taaagcagga cagaaaagtc
      901 aggcagaaaa actgaaaaag aactggcgct ttctgctaaa aaatcgtgca agcatcaacc
      961 attatgagta caaaaca
//
LOCUS       DAZKCZ010000238          959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000238 DAZKCZ010000000
VERSION     DAZKCZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.247"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(222..836)
                     /locus_tag="ACG8KN_002666"
                     /pseudo
     CDS             complement(222..836)
                     /locus_tag="ACG8KN_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          301 a          177 c          182 g          299 t
ORIGIN      
        1 tatcgatgac ttactcacaa ataatttagt tctcaaaaaa gcgtactgtg atacattcta
       61 atgacgagag cgtctttacc ggaaaaatca atcttttaga ttcaaaaaaa ctagtacttt
      121 ctataataga ttacgaaagc atcacaaaga cgaacaaaaa aaacaataaa tatagatgca
      181 gactgtagac atcatccata aaatggactt tgtctacagt ctgaaacgag atctttcttc
      241 ttacgtgaag gaagaatatc cagtaatttc ccagtatcgc catccataca agaaaaagaa
      301 tagggaccga cttgattttt caatgaacga aattcatcca ccaatgaaca gcgaggcaac
      361 caattccttt tcacgtttgt catggggagc agagaagcga gtgttcgcga aacggttgac
      421 caagaaacgc cgcaatgttt agaaatcgtc gtcattgctt gtttttcagt caattcctcc
      481 aatattttat aatgtacttg tttggcgata aaacagtttt tttcaacaat gtaggtattc
      541 gccgtaaaat aggtgtgaca gccacgacag aggtaccgtt gctttgccaa agcaagataa
      601 gtccgttgat tcgcacactt attgagaagg accagcgatt ttttctttcc gctcttaacg
      661 atcagcccat gttgcttcac ctgtttacac tgaggacagc gctgaatcgt tttaatgagc
      721 gtggcagaaa caacgatact gttgattgaa tgtatttttt tcttctcgac atgagtgatt
      781 tgaatgtttt tatctttgat tcctagtaat agtttgataa gatgaagatc aggcatacag
      841 aacgctcctt tgattgtgtt gtggtgattc aattctaacg gagcttctgt atgttttgtt
      901 tttttatgct caaaaggact gatagaatga ttttctatca gtcctagaaa ttatagcgc
//
LOCUS       DAZKCZ010000239          954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000239 DAZKCZ010000000
VERSION     DAZKCZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.248"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..417
                     /locus_tag="ACG8KN_002667"
     CDS             7..417
                     /locus_tag="ACG8KN_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9189634.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            431..787
                     /locus_tag="ACG8KN_002668"
     CDS             431..787
                     /locus_tag="ACG8KN_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9189635.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          323 a          153 c          189 g          289 t
ORIGIN      
        1 gagaaaatga taaaagtata tacttcgcct agctgtactt cctgtagaaa agcaagagca
       61 tggttgcaag cgaatcaact tgaatttgaa gagaagaata tttttgccga accgttaaca
      121 gaaagtgaga tcaaaaagat cttggcagcg actgaaggag gagttcaaga tattatttcc
      181 actcggtcga aaatttacga aaagctgggc atagatttta acgaattaac tttaaagcaa
      241 atgatcgtct tattcaaaga atatccttca ttattgagac gacctatttt gattgatgaa
      301 aaaagaatac taatcggatt taatgaagat gagatccggt catttgtccc tcgtgaaatt
      361 cgatgtgttg ccagaaatct gatctattta caaaacaata acatcttaaa ttcataggga
      421 gggagccttc gtggaagtga aagtctatgg atcagcgcac tgtgtaacaa gtctgaaggc
      481 gaaacaatgg ttagaacacc atcaattgcc ttatcaattt gttgacttag aagaacgcga
      541 tctaagtaca gaagaagcag aagaattatg cagttttgaa gaagtgcaag cagaacagct
      601 atttgcgact tggtctgagg cttttcacaa cattgcgatt gacctttcct gtgtaggaaa
      661 aaaacaactg atttttttat gcacacaaca taccaatctc ctcagacgtc ctttgattat
      721 tatcgatgaa gttctattta ttggatacaa tgagcagttg atggaacagc ttatcttaaa
      781 taaatagact ttaactgttt taatcaagtc actgagcatt tcatacagtt gtaacagtcg
      841 aacacttagc atcaagcgtg ttatgtgttc tttttttgac tttatttttc tactatagag
      901 acatgctcaa tttatgtggt caaaattcaa atctaggtgg ggatatagag ttaa
//
LOCUS       DAZKCZ010000240          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000240 DAZKCZ010000000
VERSION     DAZKCZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.249"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..697
                     /locus_tag="ACG8KN_002669"
     CDS             290..697
                     /locus_tag="ACG8KN_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189636.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            711..>943
                     /locus_tag="ACG8KN_002670"
     CDS             711..>943
                     /locus_tag="ACG8KN_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189637.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKR"
BASE COUNT          354 a          118 c          165 g          306 t
ORIGIN      
        1 ttttgagggg aagtacatat agttgttgat atagttgcta atattgttag ctaatttgtt
       61 agtaaaactg tatacgaatt tagatagtaa aaaaacagtt tgttccttat atagaggcac
      121 ttttactact gattaaatgt cagatatgct tacaaatata cttataaata tgcttacaaa
      181 attagctaat atttccgttg acaaagcttt gttactattg ttaaactaaa ttgactagca
      241 aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag
      301 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
      361 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      421 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      481 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      541 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      601 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      661 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      721 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      781 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      841 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
      901 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaa
//
LOCUS       DAZKCZ010000241          907 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000241 DAZKCZ010000000
VERSION     DAZKCZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.251"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..834
                     /locus_tag="ACG8KN_002671"
     CDS             73..834
                     /locus_tag="ACG8KN_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9189638.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
BASE COUNT          242 a          152 c          186 g          327 t
ORIGIN      
        1 aaagaaaatg tgattttttt cactatattt tatcacatga aaattcatta agaaagtagg
       61 aatcgaagta ttatgcacac accagaagaa attatggaac tcagtattca tcttggaaca
      121 aaaaaaatac ataaacctat cttagctaaa ctgattttag gttttatcgg aggagccatg
      181 atttctctag gttatttggc atacgtacga gtctctgctt ctatcccaga atctatggct
      241 agcttgacta gtttagttgg tgcctcggtt tttcctatcg gcctgctggt catccttctt
      301 ggcggtggtg aattgatcac cggaaatatg atggctgtgg cgattgcctt tatggataaa
      361 aaagtcacat tctcacagct ggttaagaat tggtcagtga ttacttttgc aaatattatt
      421 ggtgcaattt tcgttgccta tttctttgga cactttgttg gtttaactca ttcaggtgtc
      481 tatctcgacc aagtgattca tattgctgat cataaaatcg ctgtttcttg gtggcaagca
      541 ttaggctccg gaataggttg taattggttt gtcggtttag cgttatggtt atcatatgga
      601 gcaaaagatg cggccggcaa agtactaacg atttggtttc cagtcatgat ttttgtggca
      661 atcggtttcc agcatagtgt ggccaactgt tttgtgatac ctgctgccat atttgaaggt
      721 caagcgactt ggttagaatt tctctcgaat tttatccccg tttatattgg aaatatcatc
      781 ggcggagcta tttttgtttc aggtttttat tacgttagtt ataagcatca ttaagattga
      841 aggtaatgtt cattcgagca ttaccttcaa tctctttata tactatttat attttaagaa
      901 caatagc
//
LOCUS       DAZKCZ010000242          906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000242 DAZKCZ010000000
VERSION     DAZKCZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.252"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>906
                     /gene="bsh"
                     /locus_tag="ACG8KN_002672"
     CDS             <1..>906
                     /gene="bsh"
                     /locus_tag="ACG8KN_002672"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP9189639.1"
                     /translation="TSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLD
                     THYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPW
                     ILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYD
                     NPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSS
                     VSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCN
                     VDKGIYYYRTYEDSQITAIDMNKED"
BASE COUNT          311 a          121 c          164 g          310 t
ORIGIN      
        1 tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg attatgaaat
       61 atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt ttcgaaaggt
      121 aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag ctgactaccc
      181 tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa atttttctgg
      241 gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt ttgaatttat
      301 tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt taaaaaatat
      361 caatttagca aatataaatt atagtgatga acttccttta tcccctttac attggctatt
      421 agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc atatatatga
      481 taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat ttaatttaaa
      541 caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc aaataagttt
      601 gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat cctcagtatc
      661 tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt cagagtcaga
      721 aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag gtttgtgtga
      781 tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg ttgacaaagg
      841 aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata tgaataaaga
      901 agactt
//
LOCUS       DAZKCZ010000243          896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000243 DAZKCZ010000000
VERSION     DAZKCZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.253"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            253..876
                     /locus_tag="ACG8KN_002673"
     CDS             253..876
                     /locus_tag="ACG8KN_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9189640.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          331 a          129 c          167 g          269 t
ORIGIN      
        1 aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac ttaaaggagg
       61 tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt tgttgtttct
      121 tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt tttcagcggt
      181 ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata gattggatag
      241 gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca aagtttagct
      301 cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga agacgattta
      361 aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga gctgcaagag
      421 cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga tcgattagca
      481 agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg tgcttctatt
      541 gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag agacttgcta
      601 acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa aaaaatcaaa
      661 gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac tggtaaaaag
      721 attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga aattgttgat
      781 ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt tacaagaaaa
      841 accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag aaagaa
//
LOCUS       DAZKCZ010000244          869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000244 DAZKCZ010000000
VERSION     DAZKCZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.254"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..129)
                     /locus_tag="ACG8KN_002674"
     tRNA            complement(57..129)
                     /locus_tag="ACG8KN_002674"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:His,seq:gtg)
     gene            complement(132..205)
                     /locus_tag="ACG8KN_002675"
     tRNA            complement(132..205)
                     /locus_tag="ACG8KN_002675"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Trp,seq:cca)
     gene            complement(212..292)
                     /locus_tag="ACG8KN_002676"
     tRNA            complement(212..292)
                     /locus_tag="ACG8KN_002676"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Tyr,seq:gta)
     gene            complement(294..369)
                     /locus_tag="ACG8KN_002677"
     tRNA            complement(294..369)
                     /locus_tag="ACG8KN_002677"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Phe,seq:gaa)
     gene            complement(405..477)
                     /locus_tag="ACG8KN_002678"
     tRNA            complement(405..477)
                     /locus_tag="ACG8KN_002678"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(442..444),aa:Asp,seq:gtc)
     gene            complement(487..559)
                     /locus_tag="ACG8KN_002679"
     tRNA            complement(487..559)
                     /locus_tag="ACG8KN_002679"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(524..526),aa:Val,seq:tac)
     gene            complement(575..646)
                     /locus_tag="ACG8KN_002680"
     tRNA            complement(575..646)
                     /locus_tag="ACG8KN_002680"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(611..613),aa:Glu,seq:ttc)
     gene            complement(661..749)
                     /locus_tag="ACG8KN_002681"
     tRNA            complement(661..749)
                     /locus_tag="ACG8KN_002681"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(713..715),aa:Ser,seq:gga)
     gene            complement(789..862)
                     /locus_tag="ACG8KN_002682"
     tRNA            complement(789..862)
                     /locus_tag="ACG8KN_002682"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(826..828),aa:Asn,seq:gtt)
BASE COUNT          243 a          238 c          198 g          190 t
ORIGIN      
        1 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaaaagggcg
       61 aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc
      121 cacattcgcc atggcagggg cagcaggaat tgaacccaca ctaacggttt tggagaccgc
      181 tgttctacct ttaaactatg cccctataaa atggaggaga gtggattcga accactgaac
      241 cctaaggaac ggatttacag tccgtcgcgt ttagccactt cgctactcct ccatggtggc
      301 gcgggacaga atcgaactgc cgacacacgg agcttcaatc cgttgctcta ccaactgagc
      361 taccgcgcca tattttaaaa ttattaattg aataacaaga acgacggttc cgacgggaat
      421 cgaacccgcg atctcctgcg tgacaggcag gcatgttaac ccctacacca cggaaccagt
      481 tcattatgga ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc agatgctctc
      541 ccagctgagc taaacctcca acattcaatt aaaatgaccc gtacgggatt cgaacccgtg
      601 ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa cgggccaggt aaatttgcta
      661 cggagagtaa gggattcgaa cccttgagac agtttgcacc atctacatga tttccaatca
      721 tgctccttcg gcctctcgga caactctcca gagtgaaaga agctcaatac tctttctcaa
      781 aatgagaact ccggcagtag gactcgaacc tacgacatca tgattaacag tcatgcgcta
      841 ctaccaactg agctatgccg gaataacat
//
LOCUS       DAZKCZ010000245          863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000245 DAZKCZ010000000
VERSION     DAZKCZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.255"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..352)
                     /locus_tag="ACG8KN_002683"
     CDS             complement(89..352)
                     /locus_tag="ACG8KN_002683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189641.1"
                     /translation="MILQVYNEQQGLTGGSSESSSSPESSEPESAAPSTPAGGDVSSV
                     DTNGNGNVTIKEAKAAGYSMPITSDHWLYQYMNDRDNDGMVGE"
     gene            complement(611..>863)
                     /locus_tag="ACG8KN_002684"
     CDS             complement(611..>863)
                     /locus_tag="ACG8KN_002684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189642.1"
                     /translation="AEPRGKGMLKNLFFKNKKQLTILEKIFYFFVGNKNRMQNKSYYV
                     RERAGAKWRYRKFKDPWIAKNRNTLFKRVFGFFEDIFRG"
BASE COUNT          251 a          158 c          153 g          301 t
ORIGIN      
        1 agagactaaa tttccgaatg aactgccccc tgtctacttg acagggggca gttcattaat
       61 ctggtctttc atttttcatt attttggttt attctccaac cataccgtca ttatctctat
      121 cattcatata ttgatataac caatgatcac tagtaatagg catagaatag ccagctgctt
      181 ttgcttcttt aatggttaca ttcccatttc cattggtatc tacagaggaa acatctcctc
      241 cagcaggagt acttggtgcg gcagattctg gctctgagga ttcaggagaa gatgaagatt
      301 cactacttcc tcctgttaaa ccttgctgtt cattgtagac ctgtagaatc aagtgcgcaa
      361 tggaagactg gtaatttctt tattcgctat aacgatgtga ttgacagtta cattgaagat
      421 ggaaaagtga tcgaaaaaaa tggagttttg agaacaatac ttgatatttc ctataatagt
      481 ccttcgagag ttgctgaatt aatatcagga aactcaacaa atggatggtc atttgttgaa
      541 ggtttaagtg aattgcggac tatggatcag gaataaaaaa gaggcatggg cctctttttt
      601 atttggaaat ttatcctcta aatatatcct caaaaaaacc aaagactctt ttgaataggg
      661 tgtttctatt ttttgctatc catggatctt taaattttcg atatcgccac tttgcccctg
      721 cacgttctcg aacataatag gacttatttt gcatgcggtt tttatttcct acaaaaaaat
      781 agaatatttt ttctaaaatt gttagctgtt tcttattctt aaaaaataag tttttaagca
      841 ttcctttccc ccttggctca gcc
//
LOCUS       DAZKCZ010000246          830 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000246 DAZKCZ010000000
VERSION     DAZKCZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.258"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG8KN_002685"
     CDS             complement(<1..434)
                     /locus_tag="ACG8KN_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189643.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..>830)
                     /gene="tnpA"
                     /locus_tag="ACG8KN_002686"
     CDS             complement(451..>830)
                     /gene="tnpA"
                     /locus_tag="ACG8KN_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFP9189644.1"
                     /translation="VFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISESYHITLVE
                     WNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMFWSKSFCL
                     LTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          250 a          136 c          133 g          311 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg
//
LOCUS       DAZKCZ010000247          827 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000247 DAZKCZ010000000
VERSION     DAZKCZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.259"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..779
                     /locus_tag="ACG8KN_002687"
     CDS             147..779
                     /locus_tag="ACG8KN_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9189645.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          297 a          117 c          132 g          281 t
ORIGIN      
        1 gtgtctctca gggcaattct aagaacgttt caagagatag cgacactatc gttatcgaaa
       61 aaggcgaaaa cctatctcat tttctgctaa tgtaagttgg tttagctttt gttaatatga
      121 ctttatgaaa atggaggtaa ttaaatatga tttttcctaa agttaatgaa gcaagcttga
      181 ataaaggatc cttttttcat atctatgaaa acgaagattt tgaactgtat aatatccttt
      241 cacaaaaaga ggtcatcgaa aaccaaataa tctgccatcc aattatttac aaatctattt
      301 tcaagtatgc attttttgct tccaatgatt ttcaaagaat ggagattacc gactgtatat
      361 tcgaaaactg tgatttttca ggctgtatat tgacaaaaag catgttgcat cgcgtgaaat
      421 ttgtcaattg taaattaaca ggtactaaat tgatggaaag ttatattggg aacacgttat
      481 tcgagaattg taaaatggac tatgtaaatt tatctggttc aaacataaaa gaaagtacct
      541 ttgagcactc tgtcttatca agcgcagatt ttgtagactg ttccctcact aaaataatgt
      601 tttctactaa tgatttagaa aatgctagct tttttgatac tgatttaaaa aatatcgatt
      661 taagtagcaa ctcctttgaa atgattgaag tacgctcgga acatctaaaa aattgtacgc
      721 ttaatcagtt gcaagcactt ggttttgcta gaaaattttt acaaataaaa gtagtataat
      781 caaaatgaat atataagata aatttttagc aatacaaata tccataa
//
LOCUS       DAZKCZ010000248          819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000248 DAZKCZ010000000
VERSION     DAZKCZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.260"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..283
                     /locus_tag="ACG8KN_002688"
                     /pseudo
     CDS             185..283
                     /locus_tag="ACG8KN_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(506..736)
                     /locus_tag="ACG8KN_002689"
     CDS             complement(506..736)
                     /locus_tag="ACG8KN_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFP9189646.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          298 a          123 c          136 g          262 t
ORIGIN      
        1 ctagggggat taaaaataca cccgtcctca cgggtgtatg ggttactgat agacttgatt
       61 ttaactggtg tcagaaactt atgtccagaa agggtgatga aattttggca atttacggct
      121 attcaagaat tagcacaagc aaccaagatt acaaaacaca aattcaaaca ttagaaaatg
      181 ctggcgcaga aataatcttc ctagaaaatc actggattat cgaacacctt tggaagtatt
      241 tttgagttac gtaagtattg atgatctgtc taacttaatt tgacaattaa gagtatattt
      301 acttcagatg gtaaggcaac aaaataaaat acaataggca gatgaactaa taaataaaat
      361 ctgtattaaa tacagataaa ttaatataaa aatcaaaagg atcataaact tttaacaagt
      421 gatgaaatgg gtggaaattg attcttatag acagtttgtc tcataacatt gaagtatttt
      481 gcaattttag cattgaaaca cttttttaat cttcaacgtt gttcttataa acaagatgat
      541 tgggaatctt ttgaattaac actgtatagc ttagaacacc ctttatagaa aacattcgct
      601 tagcaatctt aataattcct tgtgcccgat gtcgtttaga ttcagtaggt tcctgctcat
      661 cgatcatgga aaaaatttga aggatcagat ccttaataaa tttatttaag agaggatgat
      721 ccattgcttc atccatactc gataaactag tgataatgag ctacacgttt ttctttcaag
      781 ttgatcaata gcttccacga taaaaatatc tttagataa
//
LOCUS       DAZKCZ010000249          804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000249 DAZKCZ010000000
VERSION     DAZKCZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.262"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..693
                     /locus_tag="ACG8KN_002690"
     CDS             <1..693
                     /locus_tag="ACG8KN_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9189647.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          296 a          126 c          148 g          234 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct ccca
//
LOCUS       DAZKCZ010000250          789 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000250 DAZKCZ010000000
VERSION     DAZKCZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.263"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..>789)
                     /locus_tag="ACG8KN_002691"
     CDS             complement(150..>789)
                     /locus_tag="ACG8KN_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFP9189648.1"
                     /translation="GVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQSFVQRGLFG
                     VKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVKDELKSIFKA
                     SELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRRRIRTTNVLE
                     RLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT          238 a          181 c          137 g          233 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacct
//
LOCUS       DAZKCZ010000251          773 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000251 DAZKCZ010000000
VERSION     DAZKCZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.264"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..660
                     /locus_tag="ACG8KN_002692"
     CDS             <1..660
                     /locus_tag="ACG8KN_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9189649.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          276 a          136 c          142 g          219 t
ORIGIN      
        1 tatatttctt tgttatgcaa agaagaagtt cgcgaactac ctaaatctaa ttctgctgtt
       61 ggtattgacc taggtattat agactttgca attctttcta ccggtcaaaa atttgataat
      121 aataaattta cgtccaaaat ggaaaagaaa ctcaaacgtg aacaacgtaa gttgtcaaga
      181 cgtgccttat tagctaaaca aaaaggtatc aatctgtttg aagctagaaa ttatcaaaca
      241 caaaaacgta aagtagcacg attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat tatcaaaaat cacgatatca tctgtattga agacttaaac
      361 acaaaaggca tgttgcgtaa tcataaacta gcaaaaagta tttcggatgt ttcttggtct
      421 agttttgtgt ctaaattaca atacaaggct gactggtacg gtcggaaaat catcaaagta
      481 gataagtggt ttccatctag ccaaatctgt tcgaattgtg gacataaaga cggcaagaaa
      541 tctctcgaaa ttcgagaatg gacttgccct atttgtcata ctcatcatga ccgagatatc
      601 aacgctagta tcaatatcct gaccgaaggt ctaaggctac actctatggg attggcttag
      661 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
      721 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc act
//
LOCUS       DAZKCZ010000252          772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000252 DAZKCZ010000000
VERSION     DAZKCZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.265"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..360
                     /locus_tag="ACG8KN_002693"
     CDS             <1..360
                     /locus_tag="ACG8KN_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9189650.1"
                     /translation="TPFTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLV
                     LSLTFEIIQFIFAIGATDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGIL
                     LLVLLLVYRTHLRINYV"
BASE COUNT          252 a          119 c          140 g          261 t
ORIGIN      
        1 actccattta ctgctactgg gaatttcaga gagatgatag ataatgttat aatctttatt
       61 ccatttggct tgcttttgaa tgtcaatttt aaagaaatcg gatttttacc taagtttgct
      121 tttgtactgg ttttaagtct tacttttgaa ataattcaat ttatcttcgc tattggagcg
      181 acagacataa cagatgtaat tacaaatact gttggaggct ttcttggact gaaattatat
      241 ggtttaagca ataagcatat gaatcaaaaa aaattagaca gagttattat ttttgtaggt
      301 atacttttgc tcgtattatt gctcgtttac cgtacccatt taagaataaa ttacgtgtaa
      361 gatgtctaaa tcaagcaatc tgatctttca tacacataaa gatattgaat gaattggatt
      421 agatggaaaa cgggatgtgg ggaaactcgc ccgtaggtgt gaagtgaggg gaaaaccggt
      481 gataaagtaa aaagcttacc taacactata gtaacaaaga aagcccaatt atcaatttta
      541 gtgctgagga attggtctct ttaataaatt tccttaacgt tgtaaatccg cattttcctg
      601 acggtacccc cactagcagc catgcttttt ttgaatcttt gtaggacgtt ttaaagagaa
      661 ttacccactt taaaattaaa catgcgctta gaatcgcttt aggaaacacg atccagtcca
      721 ataatcgtcg ataaaaactt ttgaaaaagg ttggtgaaat tacctacttt tg
//
LOCUS       DAZKCZ010000253          761 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000253 DAZKCZ010000000
VERSION     DAZKCZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.266"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            656..>761
                     /locus_tag="ACG8KN_002694"
     rRNA            656..>761
                     /locus_tag="ACG8KN_002694"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          313 a          124 c          134 g          190 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actctcgatg aagtagacct ttgaaaactg aacaaagtaa
      541 gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga caacaaacat ttttaacaag
      601 caagcaatat gctagcaaac aaattgagct taacaatcgc aagattgttc gaacttttta
      661 tgagagtttg atcctggctc aggacgaacg ctggcggcgt gcctaataca tgcaagtcga
      721 acgcttcttt ttccaccgga gcttgctcca ccggaaaaag a
//
LOCUS       DAZKCZ010000254          743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000254 DAZKCZ010000000
VERSION     DAZKCZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.267"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..710
                     /locus_tag="ACG8KN_002695"
     CDS             9..710
                     /locus_tag="ACG8KN_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9189651.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          259 a          142 c          129 g          213 t
ORIGIN      
        1 actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg
       61 attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt
      121 cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct
      181 tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg
      241 actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca
      301 cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg
      361 aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag
      421 tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg
      481 taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata
      541 tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg
      601 aaactgctgg acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa
      661 agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa
      721 aataagcagt gaccctgata aat
//
LOCUS       DAZKCZ010000255          737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000255 DAZKCZ010000000
VERSION     DAZKCZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.268"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..567)
                     /locus_tag="ACG8KN_002696"
     CDS             complement(4..567)
                     /locus_tag="ACG8KN_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003143765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9189652.1"
                     /translation="MSGYIYGYARVSTRSQELNRQIDLLNEQNCNEILTEKMTGTKAD
                     RPQLNRLKDKVRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHEVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQRDIERAVKLYDSQ
                     IYSIKEITEMTGISKATLYRYLKTNNF"
BASE COUNT          196 a          151 c          133 g          257 t
ORIGIN      
        1 gatttaaaaa ttatttgttt ttaaatagcg atagagtgta gctttactga tgccagtcat
       61 ttctgtaatt tcttttatgc tgtatatttg actgtcatac agtttcaccg ctctctcaat
      121 atctcgttga ttaacgcgtg ggcgtcctcc ttttcggcct ctagcacgcg cactttttaa
      181 cccttctttg gtacgctcca caatcaaatc acgttcaaac tgactaaacg cttggaagac
      241 ggtcatcatt aagcgtccgt gaggagtgct agtatcgaag ttttctttca aactcaccag
      301 cttcacttca tgttcttcaa agtaggtaac gagatcaatt aaatctttgg tactccgacc
      361 tagacgagag aaactttcca cgataaccgt gtctccagga cgcaccttat cttttagtcg
      421 attcaactga ggacgatcgg ctttggttcc ggtcattttt tccgttagga tctcattgca
      481 attttgttca ttgagtaggt ctatttgtcg attcaactct tgcgagcgtg tactcactct
      541 ggcatagcca tatatatatc cactcataaa aaatcatcct ttgtttttga tattattgta
      601 tcataaacgg gagttaaagg tgcatacata aagagagaag ttttgagact aaaaaggact
      661 actttttagg tgaaaaatcc gtcctttaaa atcgtctcat aatcgattgt ttttgagact
      721 tcaggtttaa ttttaac
//
LOCUS       DAZKCZ010000256          717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000256 DAZKCZ010000000
VERSION     DAZKCZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.269"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..701
                     /locus_tag="ACG8KN_002697"
     CDS             33..701
                     /locus_tag="ACG8KN_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9189653.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          192 a          126 c          131 g          268 t
ORIGIN      
        1 gctgaaaatt ttcaaaagaa aagaggctag aaatgaaaaa attaaattta tttacagaat
       61 tgtgcggaat cattttaggc gcaggaatct acggattagc tgtgacagga atcaatattc
      121 catctaagct ggcagatggt ggggttactg gtattgcttt gctcttgcat aatctttttg
      181 gattcgctcc ttctgtcact tcactgcttt ttaatttacc tttattgctt atctcattgt
      241 ttctttttgg aaaacacgct tttctccgga cgatcgtcgg aacatttgcg ctagttttct
      301 ttttacattt atgggagagc cttcatatac attttgctgt agatagtcta ctgatcaata
      361 gtttgatgac aggtattctt tctggcattg gttgcggtct cgtctttcga ttcggcggaa
      421 gcacaggcgg aacagatatc atttatcaag ccattgaaaa atattttcat attaagattg
      481 gcaaaagtct tttcttgatt acttttggta ttttagttgt ctccttgctt tatttggatc
      541 tcacacattt tctgtataca ttacttagct gttctattct atcttataca ttgaataaag
      601 tgaaatatct ggaaattgga tctgttttgc cccaattgga agcaaaacag actacagaaa
      661 catttgaacc acttgaccct cttgaacagt tagagcactg attcttattt ttcacta
//
LOCUS       DAZKCZ010000257          713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000257 DAZKCZ010000000
VERSION     DAZKCZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.270"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..275
                     /locus_tag="ACG8KN_002698"
     CDS             39..275
                     /locus_tag="ACG8KN_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9189654.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            272..670
                     /locus_tag="ACG8KN_002699"
     CDS             272..670
                     /locus_tag="ACG8KN_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9189655.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT          256 a          114 c          128 g          215 t
ORIGIN      
        1 ttactataga atgagaatgg tatagagggt ggcgaaatat gaaaaaaatg aaaaagtata
       61 aaattagaaa atctggcaat agcgatgtaa caactatccc accagaagta aaagaattca
      121 tgggtgtgca gacaggtgac gcaatcagtt atgtttttca atctgatggt tctgtacgaa
      181 tgatcaaagc acaagaagaa cctgatattg attcattagt tgattcgatc atgaatcaat
      241 atgaagacgc gttaaaagat ttggttgatt tatgaggtat ctttcgacta aagaaattat
      301 caaaatcaac gcgaaagtta ttttacgtca ctcgccaggc gaaatgatcg gtgtaaaaga
      361 tgcaaatgca ttagatatgg ccgtcaaaca gccttcacaa gcagtattca atcaagaact
      421 ctatcctgaa atctatgaaa aagcggcgat tttagcaatt aatttagcta aaaaacatcc
      481 ttttcataat ggcaataaac gaactgctct tgttgccatg ttgttgtttc ttcaattgaa
      541 taattgtcca gttgcttttt ctcgtcaaga agctgttgat tttatcataa tgattaccac
      601 tagttcattg gatttcgatt cactaaagtc caagataacc agttatttac gacaaaccgc
      661 aataaaatga acgaattaaa aactttattt ctcaattaga tcagcatgtc att
//
LOCUS       DAZKCZ010000258          709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000258 DAZKCZ010000000
VERSION     DAZKCZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.271"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..209
                     /locus_tag="ACG8KN_002700"
     CDS             <1..209
                     /locus_tag="ACG8KN_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HFP9189656.1"
                     /translation="NTYQLFDMINDPNEMKNLYYDKKYESIIYEMKHKLVGYLKGREE
                     GFVKNGQLIQIGISNIVSTLKSKK"
     gene            400..477
                     /locus_tag="ACG8KN_002701"
                     /pseudo
     CDS             400..477
                     /locus_tag="ACG8KN_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          286 a           78 c          122 g          223 t
ORIGIN      
        1 aaaatacgta tcaattattt gatatgataa atgatccaaa tgagatgaaa aacctatatt
       61 atgataaaaa atacgaatct attatttatg aaatgaaaca taaattagta ggatatttaa
      121 aaggtagaga agaggggttt gtcaaaaacg gacagttaat tcaaatagga atttcaaata
      181 tcgtttcaac tttaaaaagt aaaaaataaa ctaaaagtga agatgcaata aaaaatcaac
      241 atagggtata ctgagtcaaa atatactcag atacaggcaa aactttgcgt ctttatttat
      301 ttcaaaaaaa tcaaatattc ggatggtaac aaacatacta ctttgtttgg gataaaatca
      361 gatctgagtc agagtaagtt cattctgatt cagtttttta tgtctaacga cgataaaagt
      421 ttagcacata caagatggaa ttgtaagtat cccatagtct ttaccccgga gtatcgatga
      481 aaagtgattt atggaaaatt aagagtaagt aactgttggt gatagtatca ccccaaatta
      541 gtatttccag ttttatgagc tatttattaa atgattaaaa agagatgagg gtgtgagctg
      601 ttactgatac tttaaaaaaa acttttaaag tactatcaag tgattagctc ttctagagag
      661 gttaaaaaga atttgttttc tagagtagat attacttgag agtgtaaaa
//
LOCUS       DAZKCZ010000259          686 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000259 DAZKCZ010000000
VERSION     DAZKCZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.272"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..686
                     /locus_tag="ACG8KN_002702"
                     /pseudo
     CDS             1..686
                     /locus_tag="ACG8KN_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT          251 a          124 c          146 g          165 t
ORIGIN      
        1 gctaagtata cttttgaact taaattaaag atcgttcatg attacttgga tggtaaaggc
       61 ggaagtgatt atctggcaaa gaaatattcc attaaggctc catcacaagt gaagcgctgg
      121 atcaatgcct atcaagaatt tggtgaagag ggtctagtaa gaaagcgaca aaagaaaaaa
      181 tattctgttc aatttaagct ggatgcgata gagttatatc taacaagtga gctgtcctat
      241 cgtgaagttg ccaacacact taacatgaac aaccctaatt tgatcgctag ttggatgcgt
      301 caattccgtg agggtgggat cgatggactc tcaaaaacga aaggatgtcc acctatcttg
      361 tctaagaaaa atgaacccaa aaataagaaa aaatctacaa ctaaagcaac gtctaaagaa
      421 cgtgaacgca tcgaggagtt ggagaaacga gtccgttccc ttcaaattga aaatgccttt
      481 ttaaaagaat tgaggaagct gcgaaaacag gaagcccaac aacgacgaat gaagcaatcg
      541 cacgaatcat cgcaagcctc cgaggaccgt ttaaattagt tgagcttcta gaaactttaa
      601 agttccctaa ggcaatcttt atgtactggc aaaaacgatt ggaccgtaaa aatccagatc
      661 aagagattga agcggaaatg cttaaa
//
LOCUS       DAZKCZ010000260          672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000260 DAZKCZ010000000
VERSION     DAZKCZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.274"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          239 a          117 c          104 g          212 t
ORIGIN      
        1 attctgataa aattacctat aggtaattaa aaaatataaa aaaactagtt acaaccttag
       61 gacgttgtaa ctagcttgaa gcatactaga gctaactaaa atagcactag aaagcaatgt
      121 taaacccgtc caacaaactt gatttaacat tgagaccagc ttattcagct aaatatagtt
      181 gaataagctt cttttgtttt aaaaaaacaa aaggtgtact acgaataacg caagtaaaag
      241 aaatactatt acaaccagta ggctacaagt aagtattact aaacattgcg tagttagaac
      301 ctttgctcta ttgtctaaag ccacaaaatt agagatcagc tttatgatgc taagagcgag
      361 ctgcatccat gcggacatag gttcttttga cactattaat tctccttttt aggataaggg
      421 aaattgctag tagttttttc attagcacca tcccccttca tttgattgag tctagtgctg
      481 ctgtgatagt ttgctgttta atatgcttac attcttatta taacataaat gaaacaaaca
      541 catggttaga ggtcgtatca atcaaaatag tgtctcacaa acgatttatt acttactcct
      601 gctgactaac ggactgttct tcagtaactg taactatatg tttgttaaaa ccaccattac
      661 ctgtagataa aa
//
LOCUS       DAZKCZ010000261          669 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000261 DAZKCZ010000000
VERSION     DAZKCZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.275"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>669
                     /locus_tag="ACG8KN_002703"
     CDS             <1..>669
                     /locus_tag="ACG8KN_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189657.1"
                     /translation="DKFYTIFTEEYSQNKLELINNKLTSTDKSVYDLYCYPLNEFVQK
                     QPISNSILLQYHKLQKEDFLDSVGVFYTDLQANEIREIESQLAVTISVFENEAIPYSM
                     ILELYLLNFVILFVVLQIIYCIYTSDSLKKKGIKKSMGFSAMHILKEQINNIVKYFIV
                     ICSVLFILLNLYYALTNRFNISYLTISIFFFAIILVINILCVLITSFLIKFVSLEAMI
                     KNKT"
BASE COUNT          227 a           77 c          100 g          265 t
ORIGIN      
        1 tgataaattt tatacgatat ttacagaaga atattcccaa aataaattag agcttatcaa
       61 taataaactt acttctacgg ataaatctgt ctatgattta tattgttatc cgctaaatga
      121 atttgtgcaa aaacagccta tatcaaattc tattctgttg cagtatcata aattgcaaaa
      181 ggaagatttt ttggatagtg tcggggtatt ctatacggat ttgcaagcca atgaaatacg
      241 tgaaatagaa tctcaactgg ctgtaacaat tagtgttttt gaaaatgaag ctatcccgta
      301 tagtatgata ttagagttgt atcttcttaa ttttgtaatt ttgtttgttg tacttcaaat
      361 aatatattgt atttatacat ctgatagttt gaaaaaaaaa ggtattaaaa aaagtatggg
      421 cttttctgct atgcatattt taaaggagca aatcaacaat atagtaaaat attttattgt
      481 gatatgttcg gtgttattta tcctgctgaa tttgtattac gcattaacaa atcgttttaa
      541 tatttcttat ttaacaataa gtatattttt ctttgcgatt attttggtta taaacatact
      601 ttgcgtttta attaccagct ttttaattaa gtttgtttct ttggaggcaa tgattaaaaa
      661 caagacttt
//
LOCUS       DAZKCZ010000262          661 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000262 DAZKCZ010000000
VERSION     DAZKCZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.276"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..246)
                     /locus_tag="ACG8KN_002704"
     CDS             complement(<1..246)
                     /locus_tag="ACG8KN_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9189658.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRN"
     gene            276..>661
                     /locus_tag="ACG8KN_002705"
     CDS             276..>661
                     /locus_tag="ACG8KN_002705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189659.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDN"
BASE COUNT          223 a          111 c          129 g          198 t
ORIGIN      
        1 gttgcggtcg cgcggaatct ggagatgaag ttccccgtac tcggtcttga ccgtacggtc
       61 ataggagccg ttacgagaat tccccgtgtt aaaaccaatg cgatcgtact tttcgtaatc
      121 gaggaaagcc gttaactccg ttttgagaag cgtattgaca gctttttcca aatggacacg
      181 gaataattca ttcaaatcgc ctttgttcgc tagagtcttt agaatttctg tagtaaaatc
      241 gttcatatgg aagtcctctt ttctgtgaat gtgttgtggt aactctattc tacagaaggg
      301 acttcctttt ttctatttac acaaaatatt ttacactctc gtcaaatata aaaactagta
      361 cgaaattaat ttgtaaatta tttaagcttg ataaaataag caatatcgat gatgtaaagt
      421 ccaagatatt aacaagaaaa gtaagcgaac cagtaaagct aacggcacaa gataaaaaaa
      481 atggaattgt tctttatggt gatgatgcaa agtataacta taattgggat acctcctcag
      541 aagctgaggt attaagtatt cctactaccg atacttcagg ggaaaaagtc acctttaaaa
      601 taaattttat aacggcaatt tctaagttgg agtatgggaa gcaaaaaaag agagataaca
      661 a
//
LOCUS       DAZKCZ010000263          651 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000263 DAZKCZ010000000
VERSION     DAZKCZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.277"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>651
                     /locus_tag="ACG8KN_002706"
     CDS             82..>651
                     /locus_tag="ACG8KN_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFP9189660.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGE"
BASE COUNT          218 a          112 c          128 g          193 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga a
//
LOCUS       DAZKCZ010000264          643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000264 DAZKCZ010000000
VERSION     DAZKCZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.278"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>643
                     /locus_tag="ACG8KN_002707"
     CDS             <1..>643
                     /locus_tag="ACG8KN_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFP9189661.1"
                     /translation="GGRNYSGNPRVISEKIHEKKLNYRIIWLIKKEAIITDLPDYIKR
                     IDSDSFLAAFYLYTAKYWIDDSRKTIKYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPK
                     RYLSYAKKDSESITYLLSGNRYSSEKYVSAFNIVPSKILEVGTPRNDELASSKEQVFD
                     LKKKQINVLFAPTFRNNIEDNGITQLEWLGVDNLREFFKKQQQELNLMTKFHP"
BASE COUNT          249 a           85 c          108 g          201 t
ORIGIN      
        1 ttggcgggcg taattattct ggaaatccaa gagttatttc agagaaaatt catgaaaaaa
       61 aattgaatta cagaattatt tggttaatca aaaaagaagc gattataact gatttaccag
      121 attatattaa aagaattgat tccgattctt ttttagctgc cttttatttg tatacagcta
      181 agtattggat agacgatagt cggaaaacga ttaagtatgc aaaaaggaaa aaacaatttt
      241 attttcaaac atggcacggt acgcctttaa aaaaaattga atttgatgca aaagataagt
      301 tacctaaacg ttacctaagc tatgcgaaaa aagatagtga gtcaattaca tatttattat
      361 ctggtaatcg ctatagttct gagaagtatg tttcagcatt caatatcgtt ccttctaaaa
      421 tattagaagt aggaactcct agaaacgatg aattagcctc tagtaaagag caagtgtttg
      481 acttgaaaaa gaaacaaatc aatgtcttat ttgctccaac ttttcgaaat aatattgagg
      541 acaacggaat tacgcaacta gaatggttag gtgtggataa cttacgtgaa ttttttaaaa
      601 agcaacaaca agaattaaat ttaatgacta aatttcatcc taa
//
LOCUS       DAZKCZ010000265          640 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000265 DAZKCZ010000000
VERSION     DAZKCZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.279"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..282
                     /locus_tag="ACG8KN_002708"
     CDS             <1..282
                     /locus_tag="ACG8KN_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9189662.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          219 a           89 c          119 g          213 t
ORIGIN      
        1 ttaatacaag atgatttaca ccatacgcat cggttatggt taatgaataa aattaatctg
       61 tattttgggc attcttatgt ttttgctttt tatgattaca ggacaaatat aactgaagta
      121 gatttggtta tttctaatta ctacatcgac actggaaaaa cgccattact gttaatgaaa
      181 aatataccta ccgaaagaaa ctggcgtttg ttcgagaaaa cgatttatca attgaaaaaa
      241 gaaaaagagg ttgtgaaatc agcgttttgt cctgagtatt agagaaacaa acggaaaaat
      301 agtttttcat attttgccgt ttttgagcta ataccgaggg acagctgatt gaacaccatt
      361 tattgagaga ttgtgaaaaa actgtcctgc atcaagtaat aagaacagcc aagtaaaaac
      421 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcaa gagaacaccg
      481 tttattcgga aaaagggact gtgacactac atgtcacagt ccctttttga ttgaattatc
      541 tcatttgttt tctaattttt tctttatttg agaaaatagt tgaactggat aaaaagtact
      601 ggacgtgtca aaattatttt ggatcaaatt agatctatta
//
LOCUS       DAZKCZ010000266          625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000266 DAZKCZ010000000
VERSION     DAZKCZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.280"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(351..>625)
                     /locus_tag="ACG8KN_002709"
     CDS             complement(351..>625)
                     /locus_tag="ACG8KN_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9189663.1"
                     /translation="NPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
BASE COUNT          215 a          101 c           97 g          212 t
ORIGIN      
        1 aatctcatgc atttcctgat gtgattttag aaaaatttac ttgctaaaaa actgtgaaaa
       61 tgtaggagct agcatccttg tagtaaatga tcgagctctt tattatacat taaaaaagtt
      121 cagaaatttt gatgaagtag ccaactactt tttcatgagt aaagcaacac tagaaaaaga
      181 atcctccagt acttagaatt tactggagga tgtacttttg aagaagccta taggttattt
      241 aaaaaatatt actacaggaa ggaacgagaa ttacttcgtt acttataata gaacttagtc
      301 ttatatagaa ggcatagctc acattagctt atttgtccta taaaaataaa ttacggtatt
      361 ttagaaaagc tattttctct tttttcaacg atcttcagca atttttcttt atcgtcaggt
      421 aactcaagtt catgaattct agcatgacaa ttgggacata acgcgataca gttttcaatc
      481 acatcttttc caccttgcga caaatattct atatgatgac aatgtaaata aggtacaccg
      541 tcttttataa atggcccttt ttcttggcaa agttgacaaa taccttttgc aagtcgttta
      601 acatattcag atacgaaggg attac
//
LOCUS       DAZKCZ010000267          598 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000267 DAZKCZ010000000
VERSION     DAZKCZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.282"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>598
                     /locus_tag="ACG8KN_002710"
     CDS             <1..>598
                     /locus_tag="ACG8KN_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFP9189664.1"
                     /translation="NYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWLGD
                     LMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDSTAL
                     SALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFIDK
                     IVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSK"
BASE COUNT          203 a           69 c          121 g          205 t
ORIGIN      
        1 taattatttt atagatccta atactggttc aataataggt ttgcctcact taattgtgac
       61 gttacaatca cagatgtttc tagccgacac aatcaatgat agtgtttctg actttgcagg
      121 ttggttagga gatttaatga cttgctgggg tgaagttaag aaattaggaa tacaaatgga
      181 gcaaggtgta tttgcattag taggtacggc tgcaggtgga acatttagtc tagaagattt
      241 ataccaagat gttgacgcgg taaatgttta taccttagca aaaagaaatt ctgattcaac
      301 agctttatcg gctttaaaaa cgtactattg tgataagggt gctattgata gatttaatca
      361 atttgttaag aacagatttg gttcttcaga taaaatcgct tcagtaacaa atagtttatt
      421 agaaaatgac gaaacagttg atatcttagg tgtagggata atttcatttt tcattgataa
      481 aatagttaat aattcacttg ggacagattt tacttatctt gattttattg gagatgaggg
      541 gaaagttgca aatggatttt ctgaaaaaat tattcaattt caagaaaata gaagtaaa
//
LOCUS       DAZKCZ010000268          598 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000268 DAZKCZ010000000
VERSION     DAZKCZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.283"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            222..>598
                     /locus_tag="ACG8KN_002711"
     CDS             222..>598
                     /locus_tag="ACG8KN_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9189665.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEF"
BASE COUNT          224 a           94 c           86 g          194 t
ORIGIN      
        1 ttttattttg aaaatgttct aaaatagcga tttctgctgt attttcaatg aaatttgatt
       61 ggaaaaaata aaatgtgttt tactgacatt gagttatgtt ataaatttac gatacatttc
      121 ttattgtaac gtatcgaaaa caggtctata cttattcaat ggaatcgtac cagttccaag
      181 tttttaagct cgtaccaagc ttagaaagaa gaggcaatcg tatgaaaata acagaaaaga
      241 atgtaaaccc gaaatttgaa actaccgaaa gcaatctaaa aaagtttgat ttaactgtta
      301 ctttatcgaa tcaacttgta gatagcattg ttgaaatgga tgctatgcca caaaaaattt
      361 atctttacgc tataggacat cttgacccta aaaatccgcc ttttaataat accgtcaaag
      421 tatctaaaaa ggctttattt gcgttgatgt cttctagttc atcttcttca aaacattcaa
      481 gattaaaaca agttattgaa aaattgcatc agcaatctgt cattaaaatt caaagaacta
      541 aacctaacgg taagactgaa tacaaagtca tctcaccgct agaaattact gaattcaa
//
LOCUS       DAZKCZ010000269          597 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000269 DAZKCZ010000000
VERSION     DAZKCZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.284"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..373)
                     /locus_tag="ACG8KN_002712"
     CDS             complement(<1..373)
                     /locus_tag="ACG8KN_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189666.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYS"
BASE COUNT          176 a          109 c           85 g          227 t
ORIGIN      
        1 cagaataact cacgtgatgc tgtttagcaa aattttctac tgagatatat ttttcctgaa
       61 agaaagccaa taccattttc atagataaag aatccgttat ataacgagat aacagagctt
      121 tgctagaaca ttttccatta atttccagac atatatccaa ttcactgacg gttactcgca
      181 aatttttctc tagatcgaac ttttgtaaat catgtttcag cttatcgatc gtttttttca
      241 agagaaatgt cgatagcgac aactcttctt gaacattcga tattgactct gataaatttg
      301 agcttgcttc taaatattta agtaatgaat aacttctgtg atcttctttg cttaaaaaaa
      361 actcataatt cattagtcat cagctctttc tacgtatatt actttccatg cattcaccaa
      421 ctagctgggg ataaaactga tttcctggat ccgagtcact gttgtcagcg attcttgttt
      481 gtataatata catcgtcctg tctttttttt aacattttgg agagcgtttt atttggcctt
      541 tctcgcgatc aaacctgtta ttagaattat atcatttact agtatgcatt tttatta
//
LOCUS       DAZKCZ010000270          596 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000270 DAZKCZ010000000
VERSION     DAZKCZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.285"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>596
                     /locus_tag="ACG8KN_002713"
     CDS             <1..>596
                     /locus_tag="ACG8KN_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFP9189667.1"
                     /translation="QRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIK
                     KLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKT
                     GFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPR
                     KNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNS"
BASE COUNT          207 a          115 c          100 g          174 t
ORIGIN      
        1 gccaaagagc cacgtataga gcggtttgtt ccgtcttatt tcctaatggt gactttccta
       61 gtaggagtcg attcactcgc ttaagttcaa acttagctta cactatcaag attattcgat
      121 actttttcat caagaagctc accaaaggtg aactagtcgg aattatagat agttttccta
      181 gtccattatg taaaccagtt agaaatagac aagcaaaact attgaatcaa atagccaaag
      241 ttggctataa ctcaacaaaa aaatcttatt tttatggtct taaaattcat atgatcgtta
      301 ctaagactgg ctttccgatt acttactcaa tcacgaatcc aggtgtccat gacgtgaaag
      361 tgttagagac tttatcggaa gaagcgaatc ttcccaatat cctaggggac aaaggatata
      421 ttagccataa aatccatgaa aagcttgccc ttaagggcat tactatatcc gttccgccac
      481 gtaaaaacat ggataaatca gaaaaactag accacagctt acttggaaaa caaagaaaaa
      541 cagttgagac tgttttttct tccttagaaa aattaggttg tcaaaatttc aactca
//
LOCUS       DAZKCZ010000271          593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000271 DAZKCZ010000000
VERSION     DAZKCZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.286"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(318..581)
                     /locus_tag="ACG8KN_002714"
     CDS             complement(318..581)
                     /locus_tag="ACG8KN_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HFP9189668.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          104 c           79 g          186 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DAZKCZ010000272          592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000272 DAZKCZ010000000
VERSION     DAZKCZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.287"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..505)
                     /locus_tag="ACG8KN_002715"
     CDS             complement(<1..505)
                     /locus_tag="ACG8KN_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFP9189669.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCD"
BASE COUNT          164 a          120 c          105 g          203 t
ORIGIN      
        1 catcacacat gaaaaatgga tattccgagc cttctaggct ccggtttctc cactcaaaaa
       61 caaatggatc caactgtttc atcagatttg aaacgaatga tttagaaaca gtcgcaccat
      121 ccgttagcag ttcaactgtt ttagttactt tgcgagtgga gacaccagaa atgaccattt
      181 caatcatggc agcgatcaaa gctttttcgc ttcgctgata tctttcaaag atagtaggac
      241 taaacttacc gtctctggtt cgtggaacat gaagttctaa cgtaccaatt cgagttgtaa
      301 acgaacgatc gtagtaacca tttctttgtg attgccgttc ttctgaacgt tcataggcat
      361 ttgcctgaat atattgattt cgttgttctt ccatcagctg attaaacatg atagtcataa
      421 gtgtttttgc ggtatcattc gcaccgcttt cgtttataat tgcttgaatt tcttcgcttt
      481 caaaagtaaa atgtacttga gtcattgata tcagtcctat tcgagtatgt tttctgcaaa
      541 aaacattgta ctcctaactg agatcaatgg cttttttgat gcttcacttt ta
//
LOCUS       DAZKCZ010000273          591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000273 DAZKCZ010000000
VERSION     DAZKCZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.288"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..274)
                     /locus_tag="ACG8KN_002716"
     CDS             complement(<1..274)
                     /locus_tag="ACG8KN_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9189670.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDI"
BASE COUNT          180 a          107 c           72 g          232 t
ORIGIN      
        1 aaatatcaat ctgcttttcc ttagttaatt ctcgccattc ttctaaaatc atttgtttat
       61 ttctgcctag tctatctaat gattgtagat agagtaaatc tccttttcga aacatccgtt
      121 tcattaattg atattcaggg cgtttaaaat ctttgccgct ggctttttct acaaatatat
      181 attcttcagg gattcctaat tccttcgcgg aaatcagttg tcgttcgatc gattgatcat
      241 aactactgac ccggatataa aaaaattttc tcatcgtttt tatctaccct tttttcacta
      301 aaattgtttc aaaacaatca gattaaatca gaaacgtttc aaattgtatt atgtggttat
      361 tatgccatac ataaacaaag aaaatcaagg catgtcaaaa agtatacttt ttgaaacagt
      421 aagtttacac tcccgcatga ttatatttat tccgctaaaa tacataaaaa tggcaggcct
      481 taacggtctg ccattaaata aactagttat ccagtttttc ttttatgtct tccatggtat
      541 attttatttt tcacatcaga atttgacttc tttaactttt acaatttttt t
//
LOCUS       DAZKCZ010000274          588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000274 DAZKCZ010000000
VERSION     DAZKCZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.289"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(240..524)
                     /locus_tag="ACG8KN_002717"
     CDS             complement(240..524)
                     /locus_tag="ACG8KN_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9189671.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
BASE COUNT          143 a          101 c           90 g          254 t
ORIGIN      
        1 agtacggtta tataccgtct tcaaacaaag ttaattaaat aacttcttac gagggaagag
       61 ttcatctgac taactgataa gcgttggttt ggcaatctta tcgggctatg catttataaa
      121 atgtcgtcaa acattttata aatgtgtcat ggctcttttt tcgtttctat tcagttcgtt
      181 gtttcgttat atctagtata ccgcttttaa aaaaaataag caacgatttc gtgcattatt
      241 cacacgaagt cattgctttt ttcttcttcc atttctaaat ccaatgttac ttgttctgat
      301 tctgtttctg gttctggttc tgttggctca tttgggatta aatccactac tagcgttgag
      361 ttagttaact ttgcaatttg ttctagtgtt tttatggttg gatctgattt tcctgattct
      421 attcgtgaat aatttgatct actcatttct aattcttggg agaatttttc ttttgatttg
      481 gtacctctca tcttttttaa gctttctcta aatgctagtt ccaagtttct cccccctctt
      541 tttgtgtaat aaaattcacg ctttatttaa attatagctt ttaatatc
//
LOCUS       DAZKCZ010000275          586 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000275 DAZKCZ010000000
VERSION     DAZKCZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.290"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>586
                     /locus_tag="ACG8KN_002718"
     CDS             <1..>586
                     /locus_tag="ACG8KN_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9189672.1"
                     /translation="KNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKS
                     HKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGS
                     AESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVV
                     LSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIF"
BASE COUNT          240 a           50 c          100 g          196 t
ORIGIN      
        1 aaaaatatag aagaaaatga taagaattca attattgttc atgaagaatt tgctaaacaa
       61 aacaatctaa aattaggtga tgaagttgat cttgaattac tagatattga aaaaagtgga
      121 aaaataaaaa gtcataaatt taaaattata gggatctttt ctggtaaaaa acaggaaaca
      181 tatacaggat tatcttctga ttttagtgaa aatatggtgt ttgtagatta ttcaacaagc
      241 caagagatat taaacaattc agaaaataat gaaattgcaa ataaaatttt aatgtattct
      301 ggtagtgcag aatctacaga cttagcctta aacaaattga aagagcttaa aattgatgag
      361 tcaaagtatt ttgttgaaaa agattctaat gcgtttgaag agtctttaga gtcagtgagt
      421 ggaataaaat atataataaa agtaatgact tattctatta tgttaggtgg gatggttgtt
      481 ctttcattaa tcttgattct atggttaagg gaaagaattt atgaaatagg tatattctta
      541 tctattggaa catctaagat acaaattata atgcaattta tattcg
//
LOCUS       DAZKCZ010000276          575 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000276 DAZKCZ010000000
VERSION     DAZKCZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.291"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..301
                     /locus_tag="ACG8KN_002719"
     CDS             <1..301
                     /locus_tag="ACG8KN_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFP9189673.1"
                     /translation="IDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSE
                     LPIISVQDSYGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            298..>575
                     /locus_tag="ACG8KN_002720"
     CDS             298..>575
                     /locus_tag="ACG8KN_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9189674.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIG"
BASE COUNT          200 a           69 c           97 g          209 t
ORIGIN      
        1 gatagatgtt ttaatcacag attattcatc tatttttttc gattttgcgc taacaaaaaa
       61 accaattatt ctttttaact atgatgaaaa agaatatagc ttagaaagag gtttttatat
      121 acagttaagt gagttaccaa ttataagcgt acaagatagt tatggccttg ttgatgtttt
      181 taaaaaccaa caagaaaagc ttttgaaaag ttctcaagag ttacttgatt atattggaaa
      241 ttatgaaaca ggggcaagta cagaaaaggt tcttgagttg ttagagaggg atataaagtg
      301 aaaaaaacag taaaagatat attttataat gcgatatatc aagtcttttt gattgttttg
      361 cccttactta ctatacctat acttagtcgt agaataggtt caactggttt agggatttat
      421 gggtatgttt tctcaatatc acaattttta atgactgtca ttgctgtggg aatgaatcct
      481 tttcgtattc gaaatatcgc taaatcaagg aaagataaaa aagcactatc tttacaattc
      541 tggaatattt actttcttca atttttaata ggctt
//
LOCUS       DAZKCZ010000277          566 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000277 DAZKCZ010000000
VERSION     DAZKCZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.292"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..526
                     /locus_tag="ACG8KN_002721"
     CDS             <1..526
                     /locus_tag="ACG8KN_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189675.1"
                     /translation="DFSWKTDVLLYIRDFLFIGSYYHLWFLPALAFALWLVRKSQVSK
                     IKFLSGLLLCVVGLAFESYWFILSGNDSIEYIRYCYKSVFVTTRNGLFIGVPCVIIGQ
                     HLAFHKNTILNKVFKYRNFILCAVLAIGCLLVGESFIINQIALREVNNLLIFSIPLAL
                     VVVSVSLSLKIVHL"
BASE COUNT          167 a           80 c          104 g          215 t
ORIGIN      
        1 agattttagc tggaaaacag atgtattatt gtatatcaga gattttctgt ttattggtag
       61 ttactatcac ctgtggttct tgcctgcact ggcttttgct ctatggttag tacgcaaaag
      121 ccaggtttct aaaataaagt ttctcagtgg tttgttgcta tgcgtagtag gtttagcatt
      181 tgaatcttat tggtttatat tatctgggaa tgattccatc gagtatatta gatattgtta
      241 taaaagcgtg tttgtaacaa ctcgtaatgg attatttata ggtgtaccat gcgtcattat
      301 tgggcaacac ttagccttcc acaaaaacac tatacttaat aaggtcttta aataccgtaa
      361 ttttatctta tgtgctgttt tagctatagg atgcttatta gttggtgaaa gtttcatcat
      421 aaatcaaata gctttgcgag aagtcaataa tttgttaata ttttccattc cattagcact
      481 ggtagttgta tcagtatcac tgagcctaaa gatagtacat ttataaaaat aacagtacat
      541 ctgcgaagaa ttagtacgtt attatt
//
LOCUS       DAZKCZ010000278          545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000278 DAZKCZ010000000
VERSION     DAZKCZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.293"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..483)
                     /locus_tag="ACG8KN_002722"
     CDS             complement(<1..483)
                     /locus_tag="ACG8KN_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189676.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     S"
BASE COUNT          190 a           99 c           60 g          196 t
ORIGIN      
        1 actcacaaaa atactttcag aagacatata taaggaacga agttttggaa ttagtatatc
       61 gtacaaatag accaccataa aatcgtcctt gcaaccattc tcaaaaagaa ttaaatttgc
      121 tgattgtgtt acatatatca ctgaattaca ttcatggtca attccaaaaa caatgtctac
      181 ttcattttct tttctaattc gcgcagttac atttttcaaa ctttcaccat tacttataaa
      241 tgctttagtt actgtaagct ctgatgtttg ttcagatatt ccttttataa tattttgttc
      301 atcagccaca ttccccataa atccgccgat tccttttaag taataattct cacctaagag
      361 tcgcttgcat tcactgaatt gataaaaaaa ccagttaaaa aaatcttctg gaaaaataaa
      421 ttctgatcga actttattta tatttttagc tcctattaat tttataactc gattaatgtt
      481 cacttgctgc gatgaacata taactgaata aattgagttg tttaattcaa aaaaacaact
      541 ttaac
//
LOCUS       DAZKCZ010000279          544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000279 DAZKCZ010000000
VERSION     DAZKCZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.294"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>544
                     /locus_tag="ACG8KN_002723"
     CDS             <1..>544
                     /locus_tag="ACG8KN_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFP9189677.1"
                     /translation="RFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLK
                     NRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKD
                     PDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKR
                     VSYGFKSFENMRIRIFLINQL"
BASE COUNT          192 a          106 c          100 g          146 t
ORIGIN      
        1 tcggtttcat attatcaaac acatgaatca agcctttaat gagttgcgta tccgtgaaat
       61 gaatgaactt cgtaaagtag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg
      121 ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt
      181 tcgagcacca aaatacccat ttcttacgga agcgatgatg attgatcgat tgcttggctt
      241 ttctacgtcc ctgaaggagg cgtatcccta ttttcatgaa ttagttgaag cctttcgaga
      301 caaagaccct gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacgg
      361 ctttcgggaa aagctccaaa accttctgac ctatgaagaa ggcatcacca acgcgatgat
      421 ctatccttac accaatggaa aaatagaagc gaagaatacc catataaaga caatgaaacg
      481 agtatcctac ggatttaaat catttgagaa catgagaatt agaatctttt tgatcaatca
      541 atta
//
LOCUS       DAZKCZ010000280          539 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000280 DAZKCZ010000000
VERSION     DAZKCZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.295"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..380
                     /locus_tag="ACG8KN_002724"
     CDS             <1..380
                     /locus_tag="ACG8KN_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9189678.1"
                     /translation="EICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQ
                     DLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQ
                     KFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          199 a           93 c           81 g          166 t
ORIGIN      
        1 aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa gaagcgaata
       61 ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg acgcagtcac
      121 tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt cgtagaacca
      181 tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac agccgtagaa
      241 aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt tttgatacat
      301 acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc gatacattag
      361 tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt acacaaaatt
      421 attgacgctc ccatcctaaa acataagcta tttgtttact tctgtagaat atatactttg
      481 ttgttagata agttgcacat gtttatagaa tctaaataat acccgtggtg ctagttaat
//
LOCUS       DAZKCZ010000281          522 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000281 DAZKCZ010000000
VERSION     DAZKCZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.296"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>522
                     /locus_tag="ACG8KN_002725"
     CDS             <1..>522
                     /locus_tag="ACG8KN_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HFP9189679.1"
                     /translation="KALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAK
                     TTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVL
                     VKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAES
                     TYKSLKVEFVYQYT"
BASE COUNT          187 a           86 c           96 g          153 t
ORIGIN      
        1 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
       61 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      121 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      181 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      241 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      301 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      361 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      421 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      481 acttataagt cgttgaaagt agaatttgtc tatcaataca ca
//
LOCUS       DAZKCZ010000282          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000282 DAZKCZ010000000
VERSION     DAZKCZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.297"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..161
                     /locus_tag="ACG8KN_002726"
                     /pseudo
     CDS             1..161
                     /locus_tag="ACG8KN_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            240..>514
                     /locus_tag="ACG8KN_002727"
     CDS             240..>514
                     /locus_tag="ACG8KN_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP9189680.1"
                     /translation="MDHFKGKRFQKDVIIVAVGYYLRYNLSYRDVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYTIWKKKNKQSFYSWKMDETYIKIKGKWHYLYRA"
BASE COUNT          167 a           89 c          108 g          150 t
ORIGIN      
        1 cgtattcttt aacagacgtc aagctttttc atacagaccg gggaaaggaa tttgataacc
       61 aaaccattca tgagattctg aatggttttg gaattactcg ttcattgagt aggaagggtt
      121 gtccgtatga taatgccgtt gcggaatcaa cccataaatc tgtcggttct gttgcaaagt
      181 tttctgataa actgatttta aggtaaaatg aatggaaaag atagcttgga ggaatcaaga
      241 tggatcattt taaaggcaag cgatttcaga aagatgtgat tattgtcgcc gtaggctact
      301 accttcggta taatcttagc tatcgtgacg tccaagaaat cctatatgac cgtggcatta
      361 acgtttctca caccaccatc taccgctggg tacaggaata tggaaaacta ctttacacta
      421 tttggaaaaa gaaaaataaa caatccttct attcgtggaa gatggatgag acctatatta
      481 aaatcaaagg gaaatggcac tatctttatc gagc
//
LOCUS       DAZKCZ010000283          508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000283 DAZKCZ010000000
VERSION     DAZKCZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.298"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..448
                     /locus_tag="ACG8KN_002728"
     CDS             <1..448
                     /locus_tag="ACG8KN_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9189681.1"
                     /translation="ILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEH
                     TYLGEVYLAGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDIYNQEENQTRHIR
                     NLSDAKLEEEARKVSNYNKKMAAKYVNEEEFCFSKTKIYKRTRGAS"
BASE COUNT          215 a           62 c           85 g          146 t
ORIGIN      
        1 tattttacaa tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa
       61 taaagtttta gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa
      121 accaaaagaa cacacgtatc tgggagaagt atatttagca ggacaaatat atactgttaa
      181 tgaaaaagat agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa
      241 tcaagagcaa ttgattgaag ataaagatat atataaccag gaagaaaatc aaactagaca
      301 tattcgtaat ttatctgatg ctaaattaga ggaagaagca agaaaagtta gtaattataa
      361 taaaaaaatg gctgcaaaat atgttaacga agaggagttt tgcttctcaa aaacaaaaat
      421 atataaacgt acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc
      481 ttcaaaacgt cgttgtgctc gacttaat
//
LOCUS       DAZKCZ010000284          491 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000284 DAZKCZ010000000
VERSION     DAZKCZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.300"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..491
                     /locus_tag="ACG8KN_002729"
                     /pseudo
     CDS             257..491
                     /locus_tag="ACG8KN_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          171 a           87 c           71 g          162 t
ORIGIN      
        1 atcagtcgca atattattct tttcataaat attgtaaata gaagtattaa aaatatatac
       61 aaaaagtatt atttcgagaa agataaatat tttcaaaaac cttttcataa tcctcaccct
      121 ttcttacact gataaatcat gcgtctaata ttcaaattat actcgatatg ctttagctaa
      181 tcagccaact ctgtttgtct actatcatta tatcaataga caatatctgc tcccgtcttt
      241 atgatcgtgc caaagcaatc aaaagaaaat tgcgaatcac ttagatcgaa agtttactca
      301 aacaaaagca ttgtcagcgg ttgtcacgga tttaacttat gttcgggtcg ggacaaattg
      361 ggcttatatt tgtttgatga ttgatttatt caatcgtgaa attattggcc attcttgcgg
      421 aaataaaaaa gatgcgcagc ttgtaaaaag ggccattcaa agcattcctt actcacttca
      481 agaaatcgaa c
//
LOCUS       DAZKCZ010000285          474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000285 DAZKCZ010000000
VERSION     DAZKCZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.302"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..474
                     /locus_tag="ACG8KN_002730"
                     /pseudo
     CDS             54..474
                     /locus_tag="ACG8KN_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          195 a           77 c           91 g          111 t
ORIGIN      
        1 acagaaaaat taaggtgtgt agactagaag aaaacatacc aggaggaatt tttatgtcta
       61 agagaacacg aagaactttt tcacaagaat tcaagcaaca aatcgtcaat ctttacttag
      121 ctggaaagcc acgtgtagaa atcattcgag aatatgaact aacggcttca gcatttgaca
      181 aatgggtaaa gcaatctaaa acgagtggtt cattcaaaga aaaagataat cttacgcctg
      241 aacaaaaaga attgttagaa ctacgtaaaa gaaaccagca attagaaatg gaaaatgata
      301 ttttaaagca agcagcgctg atattcggac gaagagacaa gtaatcgatg cgaataagca
      361 tctttaccct atatcagcga tgtgcagaat attaggtcta tcacgtcagt cctattatta
      421 tcaatcaaaa ccaaagaaag acgaatcaga acttgaagaa gtagtcgctg aaga
//
LOCUS       DAZKCZ010000286          474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000286 DAZKCZ010000000
VERSION     DAZKCZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.303"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..390
                     /locus_tag="ACG8KN_002731"
     CDS             91..390
                     /locus_tag="ACG8KN_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9189682.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          178 a           58 c           96 g          142 t
ORIGIN      
        1 tccagtaatg atttaaaaaa atctagatgt agcgtatact ttgtttatag aatgtagtgg
       61 gaagaaatat atttttggga ggaaatcgtg atgtctaaaa gtgaagaaat agaaaacatg
      121 gtactaaaat tatatgatct cgttttaaat ccagagatca aagaaaagga acgtatactt
      181 ttgatagacg caaagacagg cttagaaaaa ggtcagtatt atcctaaagt cataaataat
      241 ttggaacgta gtttacgtcc gttagcaatc aggggagaat tgtctaaacc agtatcaccg
      301 ttttatatgg agatatcaac aataggaaaa tttgaaaaag aattaggtcg aggaatggct
      361 tcggcaccga ttacttttgg gcatttataa aaacgagtta ttcatctcaa tgaattaaaa
      421 agtatagtca atctcataat gcaggctagg ttcatttatt tgaacagagt ctgt
//
LOCUS       DAZKCZ010000287          462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000287 DAZKCZ010000000
VERSION     DAZKCZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.305"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          158 a           63 c           64 g          177 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ct
//
LOCUS       DAZKCZ010000288          453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000288 DAZKCZ010000000
VERSION     DAZKCZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.308"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..240
                     /gene="relB"
                     /locus_tag="ACG8KN_002732"
                     /pseudo
     CDS             17..240
                     /gene="relB"
                     /locus_tag="ACG8KN_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
BASE COUNT          160 a           65 c           96 g          132 t
ORIGIN      
        1 aggcaattat agggctatga ttacggttcg tgtaagtgac gccaaaaaag aatggctttc
       61 ttatatggct gaattttatg ggattacttt atctgaactt ctaaaaaact attcgatgga
      121 acaactagaa gatgaataca atcgtcaaac ggctgaaatt gcccataaac attggataag
      181 aagtgggaaa acaacagtat tcatggaaga atccttaatg aatttggtgg gctggaatga
      241 cctgccgttt aaaattcacg cccgaaattc agaagcagct ctgattggca aatacgtgta
      301 ttggtagaga tagaagataa tcaattcgtt gttaccgcta ttcaagtagg gcaccgaaga
      361 aatgtctatg attaagagac tgtttatttt tacaaaaaaa taataactgg aatctagtta
      421 aaactcttga agtgagagat ttgactagat ttt
//
LOCUS       DAZKCZ010000289          443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000289 DAZKCZ010000000
VERSION     DAZKCZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.309"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..345
                     /locus_tag="ACG8KN_002733"
     CDS             133..345
                     /locus_tag="ACG8KN_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189683.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
BASE COUNT          141 a           77 c           88 g          137 t
ORIGIN      
        1 aaaaacacta tatattccga aaagtgagta tctccattca taaaataatc aacatattta
       61 aaaaatctaa ttagcttttt tagtcaatct aaagttcttt attctatgat ataatgttac
      121 tgtagaaagt ttgtggcggt ttggcaaatc caattgaagg agcgtggtgc ctatgggctt
      181 attagcttat cggacgtact ctgtagaaag gaaatgcctg atttgttctc attggatcaa
      241 atcattgcta ttatcggagc agcagcagta gttattactg ccgtagcgaa agctagcgat
      301 tcgattttag cttggttaaa gttcaaaaaa gaacaaaaaa aatagagccg tctcaacttt
      361 tggcgaggta gacgagctct aaagctttat ttacatgcca tccgctttga acggactaca
      421 ctgggcatgt gacagcatgc cct
//
LOCUS       DAZKCZ010000290          435 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000290 DAZKCZ010000000
VERSION     DAZKCZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.311"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..285
                     /locus_tag="ACG8KN_002734"
     CDS             <1..285
                     /locus_tag="ACG8KN_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9189684.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          131 a           79 c          100 g          125 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaagatcaag ccttgaatga gataaattct
      421 tgctcgaaag ttatg
//
LOCUS       DAZKCZ010000291          431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000291 DAZKCZ010000000
VERSION     DAZKCZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.312"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>431
                     /locus_tag="ACG8KN_002735"
     CDS             <1..>431
                     /locus_tag="ACG8KN_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9189685.1"
                     /translation="KKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDI"
BASE COUNT          139 a           77 c           93 g          122 t
ORIGIN      
        1 taagaaaaac aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga
       61 atatattcaa agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc
      121 gtttcctatt agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga
      181 ttcttctgac ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg
      241 aaaacaaact ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa
      301 tgagtttggt caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat
      361 taccttagtt gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc
      421 gattgatatt g
//
LOCUS       DAZKCZ010000292          428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000292 DAZKCZ010000000
VERSION     DAZKCZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.313"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG8KN_002736"
     CDS             <1..390
                     /locus_tag="ACG8KN_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9189686.1"
                     /translation="NLIDYLSPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGG
                     QQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKE
                     VADSADIILELSGKKLKKVNKMNLEVE"
BASE COUNT          182 a           40 c           80 g          126 t
ORIGIN      
        1 aatttaatag attatttatc accaattgaa aatattagat tagtaaataa atcagcagat
       61 gaaagtatct tgtttgaact aggtttagat aaaaaacaaa taaaaagaaa tgttatgaaa
      121 ttatctggtg gacagcaaca aagagtagct attgctagag cattggtatc agatgctcca
      181 ataatactag ctgatgagcc tactggtaat ctagacagtg ttactgctgg agaaataatt
      241 aatatattaa agacattagc taaagataga aataaatgtg taatagttgt aacacatagt
      301 aaggaagtag cagattctgc ggatatcatt ttagaactaa gtggtaaaaa gttgaaaaaa
      361 gtaaataaaa tgaatttgga ggttgaataa tgataaaaaa tgcatttgct tatgtaacta
      421 gaaaaagt
//
LOCUS       DAZKCZ010000293          427 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000293 DAZKCZ010000000
VERSION     DAZKCZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.314"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..427)
                     /locus_tag="ACG8KN_002737"
                     /pseudo
     CDS             complement(62..427)
                     /locus_tag="ACG8KN_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          129 a           74 c           68 g          156 t
ORIGIN      
        1 acttgtattg tgcaatagtt ttttacaaaa aaaaggggtg cggatatttt tttggacctt
       61 tttaacaaat atatctttgc ttcttccgat attgaagagg gcttagccct cctaaagata
      121 acttaattcg ttgattatta taccaatgaa tataattatc caatttgttt ataaactgat
      181 tgattgttgt attcttccag tcacgttgat aaaacatctc gttttttaat cgtccaaaga
      241 aaccttcgca cgctgaatta tctggtgaat aacccttttt agacatagag tgaagcaagt
      301 tgtctttgtt ccaatagtcc aactaacaat agcaccatcg taacaatcga caagtggtga
      361 taagtagatt ttccctgcgg gaattctaaa ttcagtaata tctgttaacc atttttcatt
      421 tggcttc
//
LOCUS       DAZKCZ010000294          419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000294 DAZKCZ010000000
VERSION     DAZKCZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.315"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(322..>419)
                     /locus_tag="ACG8KN_002738"
     CDS             complement(322..>419)
                     /locus_tag="ACG8KN_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9189687.1"
                     /translation="LKAATINNNVFNASQNPFGGQCIVISDDLPF"
BASE COUNT          137 a           76 c           75 g          131 t
ORIGIN      
        1 agttatgtta taatagtttt gctgtctgct accccggcct aatctggcaa ggggaggagg
       61 agatataatg aaataccttt ttgattttgt ttttgcaatc aagccacttg cagatgtagt
      121 gtgcagttac ctctgcaaat ggcttgattg caaattgcca aaaaagacag taagcctacc
      181 cgttaattct ataatataga aacgtaaaaa aaaccccatc atcctgcacg ctgatggggt
      241 ttggtgatat aatgaaatac ctctttgtta ctcataatta taccataatt agtcacgaat
      301 gcaaccaata gaaaaaacta cttagaacgg tagatcatcg ctaataacaa tgcattgtcc
      361 accaaatgga ttttgtgaag cattaaatac attgttatta attgtcgctg cttttaact
//
LOCUS       DAZKCZ010000295          417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000295 DAZKCZ010000000
VERSION     DAZKCZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.316"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..375
                     /locus_tag="ACG8KN_002739"
                     /pseudo
     CDS             1..375
                     /locus_tag="ACG8KN_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          128 a           75 c           87 g          127 t
ORIGIN      
        1 gagaaagatt tgatgattac agaggtctct tacgagaccc ttcagaagaa aaagacgttg
       61 gtcgtcgatg ctgttctctc gcctactcct cgtgcttgta gaagttgtga ttctactgtg
      121 gtagatggaa acgggaaagc aattatggtg aaaaatggga agaaggaaac gattgtccgt
      181 tttgaacaat aaaatcatat gcctttggtt atgcgcctaa aaaagcagcg ctatacctgt
      241 aaaaactgtc gcacccattg gactgctcaa agttattttg tccaacccag acattcaatc
      301 gcaaatcatg ttagatataa aattgcttct ttactgactg aaaaagtatc tttatctttt
      361 attgcgaaaa gctgttaggt atctttgacc accgttattc gtacattgaa agagttt
//
LOCUS       DAZKCZ010000296          389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000296 DAZKCZ010000000
VERSION     DAZKCZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.317"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..247
                     /locus_tag="ACG8KN_002740"
     CDS             53..247
                     /locus_tag="ACG8KN_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189688.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          171 a           51 c           69 g           98 t
ORIGIN      
        1 acttacatat atttgaatat taactaaaaa cagcaagaaa gaagaggatc aaatgtatta
       61 tgtagaagta aaaacaaaag gcgtcaaaaa caaacaatat gtgaaaggta tcagtaacga
      121 atatccttta cttggttcat ggaaagaagc tgctcctttt tccaaaccat gtgccataaa
      181 aataaagaac gagctagaaa aagaactgac ttgtggcaaa gctgttgtag atatcatcga
      241 aaagtaaatc caatgtatag aaaggaagat cacacatgaa aagcgacgta caaattgcta
      301 agattataga attagaagaa gaagacgcag tgttgacaat tgaaaaaaaa taaatgtttg
      361 tataccatat gattatttca atatgtatc
//
LOCUS       DAZKCZ010000297          387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000297 DAZKCZ010000000
VERSION     DAZKCZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.318"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..247
                     /locus_tag="ACG8KN_002741"
     CDS             104..247
                     /locus_tag="ACG8KN_002741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189689.1"
                     /translation="MKKYNRSYGEFEEKATRKEFMTVLQWVAEESNRKQRKIAGLDKE
                     EEQ"
BASE COUNT          152 a           48 c           84 g          103 t
ORIGIN      
        1 gttcttgctg tgacagatat cgaccttata ttgcgagaca cttatactta aaacgaattg
       61 gatgtgaagt tacttgattg aaagagaaga agctgaggaa ataatgaaaa aatataatcg
      121 cagctatgga gaatttgaag aaaaggctac tcgaaaagag tttatgactg ttttacagtg
      181 ggtagctgaa gaatctaatc ggaaacagcg aaagattgcg ggattggata aagaggaaga
      241 gcagtaggca aaagcccact gttcttctta gtaaaaatgc ctaaaaagga agtaaatgaa
      301 ttttaaattg taatcctaaa atctgtttta caagaagcta ctgatagtga gtatctagta
      361 caaataatat ttaaaaaaga ccgacta
//
LOCUS       DAZKCZ010000298          380 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000298 DAZKCZ010000000
VERSION     DAZKCZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.319"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>380
                     /locus_tag="ACG8KN_002742"
     CDS             <1..>380
                     /locus_tag="ACG8KN_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9189690.1"
                     /translation="TIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFS
                     RFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGL
                     PKSMDFRKVNQTFISSVSNQRNHIP"
BASE COUNT          123 a           77 c           63 g          117 t
ORIGIN      
        1 acgatcgttg gcattcatca taaaagtgcc gtcattactt tagttgaaag attatctaaa
       61 gtcattatca cgattaaacc caacggccgt aaggcattag atattgaaac tgcccttaat
      121 caatggtttt ctcgcttccc taaaaacttc tttaaatcta ttacgtttga ctgtggaaaa
      181 gaattttcta actggaaagc cattagtaac caacatgata ttgatatata ttttgcggac
      241 cctggaacac cttctcaacg cccattaaac gagaattcta acgggattct gcgtcgtaat
      301 ggactgccga aatcaatgga ttttagaaaa gtgaatcaga catttatttc cagtgtcagc
      361 aatcaacgta atcatattcc
//
LOCUS       DAZKCZ010000299          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000299 DAZKCZ010000000
VERSION     DAZKCZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.320"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          159 a           50 c           62 g           97 t
ORIGIN      
        1 tagtatttta aaaaacatat aaaatagttg ttgacatgtt agaaatgaca tgataagata
       61 actgagttgt taaaaaagaa ccacttgtga agcttgattc tatcgaaaaa aacttcaaaa
      121 aagttcttga caaataacaa atgataagtt atgataaatg agttgctgta agatttcgaa
      181 aaacgaaatc aaaaaaactt ttaaaaaaga gttgacaatt acttgtctct ctgatatgat
      241 ataaaagttg ctacggcagc caaaaacgaa tcggggaaca ctcctgatga agtagacctt
      301 tgaaaactga acaaagtaag acaaaccaaa tgtgtagggc gtcttgattc aattcaagac
      361 aacaaaca
//
LOCUS       DAZKCZ010000300          348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000300 DAZKCZ010000000
VERSION     DAZKCZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.321"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..>348
                     /locus_tag="ACG8KN_002743"
     CDS             41..>348
                     /locus_tag="ACG8KN_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9189691.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     L"
BASE COUNT          116 a           45 c           73 g          114 t
ORIGIN      
        1 tgttatccaa aaataataaa aaattggagt gtttaggata atggataaaa agaggttttt
       61 agatcttatt tcaaaaggtt tgattgtttc ctgtcaagca ttacctggtg aaccatttta
      121 caaagaggaa gaaagtttaa tgccttattt tgctttggct gctcaaaggg ctggagcagt
      181 tggtatacgt gcaaattctg ttcgagatat tattgaaatt agaaagtgtg tatcattacc
      241 aatgattgga attattaaaa gagattatcc accagaatct ccattcatta ctgccactat
      301 gagagaagta gatgaattag tagaaaccgg tgttgaggta atcgctct
//
LOCUS       DAZKCZ010000301          339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000301 DAZKCZ010000000
VERSION     DAZKCZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.323"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..319
                     /locus_tag="ACG8KN_002744"
     CDS             89..319
                     /locus_tag="ACG8KN_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189692.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          131 a           46 c           55 g          107 t
ORIGIN      
        1 atcagtcaaa ttatgcccct tttttgcccc tcaaatatcg ctttacaaca agaacgtaag
       61 ttcgtataat cgttttgagg tgatttttat gatggaagaa tttattagaa aaaatatcag
      121 tgatgaatat gcagactttt atgatcaaag cagtaaaaaa gataaattcc agatggatgt
      181 ttcaatctta gctatattag ctttttccga aaataaacaa cctgtaactg caaaaaaaga
      241 aacagtattc tctgaaggca aaataaaaac tcgatatata ttagaggtag aaactaattt
      301 taaaaacaga tcggagtaat ggttatgctt tttaatgag
//
LOCUS       DAZKCZ010000302          325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000302 DAZKCZ010000000
VERSION     DAZKCZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.denovo.324"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..>325
                     /locus_tag="ACG8KN_002745"
     CDS             51..>325
                     /locus_tag="ACG8KN_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFP9189693.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRA"
BASE COUNT          124 a           43 c           64 g           94 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagc
//
LOCUS       DAZKCZ010000303        13748 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.guided.067, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000303 DAZKCZ010000000
VERSION     DAZKCZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.guided.067"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..489
                     /locus_tag="ACG8KN_002746"
     CDS             <1..489
                     /locus_tag="ACG8KN_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9189694.1"
                     /translation="EKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYAAGNGK
                     TGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEIIEASDA
                     AFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAALKEILD
                     QE"
     gene            complement(740..2698)
                     /gene="nagE"
                     /locus_tag="ACG8KN_002747"
     CDS             complement(740..2698)
                     /gene="nagE"
                     /locus_tag="ACG8KN_002747"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFP9189695.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2695..3567)
                     /locus_tag="ACG8KN_002748"
     CDS             complement(2695..3567)
                     /locus_tag="ACG8KN_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9189696.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3909..4781)
                     /locus_tag="ACG8KN_002749"
     CDS             complement(3909..4781)
                     /locus_tag="ACG8KN_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9189697.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4909..5478
                     /locus_tag="ACG8KN_002750"
     CDS             4909..5478
                     /locus_tag="ACG8KN_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP9189698.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5545..6282
                     /locus_tag="ACG8KN_002751"
     CDS             5545..6282
                     /locus_tag="ACG8KN_002751"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9189699.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            6513..7781
                     /locus_tag="ACG8KN_002752"
     CDS             6513..7781
                     /locus_tag="ACG8KN_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9189700.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            8268..8732
                     /locus_tag="ACG8KN_002753"
     CDS             8268..8732
                     /locus_tag="ACG8KN_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9189701.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8716..9345
                     /locus_tag="ACG8KN_002754"
     CDS             8716..9345
                     /locus_tag="ACG8KN_002754"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9189702.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            9699..11177
                     /gene="msr(C)"
                     /locus_tag="ACG8KN_002755"
     CDS             9699..11177
                     /gene="msr(C)"
                     /locus_tag="ACG8KN_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9189703.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            11224..11670
                     /locus_tag="ACG8KN_002756"
     CDS             11224..11670
                     /locus_tag="ACG8KN_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9189704.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11869..12384)
                     /locus_tag="ACG8KN_002757"
     CDS             complement(11869..12384)
                     /locus_tag="ACG8KN_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9189705.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            12501..13702
                     /locus_tag="ACG8KN_002758"
                     /pseudo
     CDS             12501..13702
                     /locus_tag="ACG8KN_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         4307 a         2500 c         2826 g         4115 t
ORIGIN      
        1 gaaaaaatga ttaaggattc aggtatttcc catacatttt tacggaataa ttggtatgta
       61 gagaatgaat tacctatcat tgggcaggct ttaaaaactg gtcaattcgt ttatgctgca
      121 ggaaatggaa aaacaggttg ggcattgaaa cgtgaatatg cagaagctgc agcaaaagta
      181 ctagtagaaa gtgactctcc agaaatctta gaattatccg gcaatctaac tgactatgaa
      241 gaattagcca aggcattaga gcgtgcgaca ggtaaagagt tagaaattat tgaagcaagt
      301 gatgcagcgt ttgtcgaaaa tctaaaagaa gccggattcc ctcaagaagc agctgacatg
      361 tttttagcga tccaacatga tattaaaaag gatcagttag ctatttcctc tgatgattta
      421 gaaaaagtat tgggaaaacc attaacttct cttgaagcag cattaaaaga aatattggat
      481 caggaataat gtataaagag gttgtgaaaa agccgattag ttttgagtat gaagaaaaat
      541 tttgtgaaaa agctgttctg catcaagtaa taagaagagt caaataaaaa atagctcttc
      601 atatgtttcg tttagctgga cttattaccg cggagttggc aggagaacgc cgtttttatt
      661 cggaaaaagg agctgagaca agatttttgt ctcagctcct gtttgtttat cgataaaaaa
      721 gtctggggaa gacgatggct caggctttgt tggaaacaga gccgatttta tcatttgctt
      781 ttacatcacg tgcgggttcg atttctaagc tttggacttt gtcgccattt gtgaatacaa
      841 cgaccatatc cgtgtttttg cctgcagatt gaatagcggc aagatcaact aaagcaatca
      901 attgacctct tgcgactttt tgaccttctt ctacatataa agtgaatggt tcgcctttta
      961 agtcaacagt gttgatgccc atatgaagca agacttcgat accagcatct gtttggattc
     1021 cgacagcatg tttcgttggg aaaatgtttg tgatcgtacc agcgattgga gagaagattt
     1081 ctccattttc tggtaataca gcatagccgt cacccatcat tttttccgcg aatacatcat
     1141 ctgaaacttc agtgattgga atcagtttcc catttgcgat agcgtaaata tcttgttcga
     1201 tcgtttcacc ggcaaatgtt tctgttttaa ccacggtgtt tgtttgtgca ggtcgtgctg
     1261 cagcaccacc atgtagtttt gccatctcat ctgcaacgaa ttgtacttct gtacctacga
     1321 tgacttgcgc attttttccg tcgatgatct tcataccagg tacgccagtg gctttgattt
     1381 tattttgatc gatcaggcct gtatctttga cttgcaaacg aaggcgtgta gtacagttat
     1441 cgatcgaagt tacattgtcg gctccgccta atgcagcgta gattttgctt gcttgtgccg
     1501 catatttatt gtctgaatta gtcgttgcag tatcgtcatc tgaaagatct gcttcctcgc
     1561 ggccaggagt catcagatgg aattttttga tagcaaagtt aaagccaaag tagtaaatca
     1621 ctgctgttac taatccttgg acgatcaaca tcaatggctg attcgcaatc ggatttttca
     1681 aagataggaa gtaatcgaca aatccggcac taaaggcaaa tcctgctgtc caatggaaga
     1741 atgcactcac tgctaaagaa atcccagtga aaattgcatg caagacatat aatggccaag
     1801 caacgaacat aaatgaaaat tcaagtggtt ctgtcacacc agtaaagaat gaagcaaaag
     1861 ctgctgctag catcaaggaa gctgtttttg ctttgttttc tggacgtgca tttctgtaaa
     1921 tagcatatgc tcctgctgga agcccgaaca tcatgattgg gaaaaatcca gcttgataca
     1981 ttcctgttac gccttttgtt ccttcagaag ccaaaaattt accaatatca ttgattcctg
     2041 ctacgtcaaa ccagaacacg gagttcattg cttgatgcaa tcctgtaggg atcagtaaac
     2101 ggttgaagaa gccataaaga cctgcaccac cccagcctaa actcaaaatg aatttagcaa
     2161 aagaaactaa agcagagtaa accggaggcc atacgagcag tagcactgca ctgatcagca
     2221 acatgacaaa ggcagccaag attggcacta gacgcttgcc gctaaagaaa gataaagcca
     2281 tcggtaattt tgcttcgtgg aaacgattat aaagagctgc cgcaatcaat ccggcaataa
     2341 taccaataaa tacgttactg atctgagcaa aagatggatc aactgattct ggtttgatat
     2401 tcaataaagt agctactgaa tcagttttta aaacatttac tggaacttca aatgcgacca
     2461 aacctgccaa ggcagcagca cctgatttgt ccactgacat ccctagtgct aacccaacgg
     2521 caaacagcaa aggtaattga cctaaaactg cgttaccgcc agcttgtaaa aaatgagcga
     2581 caatgtcgtt actataagat gcccaccagt ttccgatccc tactagtaaa gcagcagcag
     2641 gtaagaccgc aactggaagt tgaagggaac gtcccattcg ttgtagatat gctttcaatt
     2701 tttttcctcc tcatgattgt taacatttgt ttgtacgatt gcgcttctta aacgctcgat
     2761 atggatggcc aagtaaccca gttcttcttt cgtgatcgcg agctggtatt ctttggtcag
     2821 caacacacgg atttttttag aaatcttata gctttctggg tattttcgtt tgaccatttc
     2881 tacgatttcc gtgtctaaaa cagcatattg ttgcgcatgg gttcgttgta gaagcagtcg
     2941 taaatgattc accagtcttg aataattcag tgaaaatggt tctgaatgga tatctgtttc
     3001 caattctgat tccactaaat ggatgatgtc agatacaatc gtcacttcac ggatactgcg
     3061 atggttattg ttccgaccgc ttcgtccgct gtgaatatgg attgcgatat atcctgcttc
     3121 gtcataagta tatggaatcc caagctcttt tgtcagatac tctactgccc attgagcaat
     3181 actgaattca tcactgtaaa gcacttcgat ttctcttaaa agtttgttgc ggatgacgat
     3241 accgttatta atgttctccg cagaaaaagc aatgtggtcg gtcaaagcga tcaatacatg
     3301 ttcattcaac ttttccatca aaacagtttc cgcatgatca atgatcgttt ctgctgcaaa
     3361 taaaaagcgt tcatcaatct gattaagtaa attttgcaac ttgatttgac cttctggttc
     3421 catgacaaat atccgttcga catcacgatc aaaaatcaaa tcatttcttt ttctgtcaaa
     3481 tccaatccct tttccaatag ctactttttc ctgtccaccg tcatctacca atactgcatt
     3541 ttgattaaag atctttttga ttttcatgcg cgcacccttt ttgaagattt ggttttcacg
     3601 tttgctagac cagttgattg cggccaaaca aaaatagaca cgaaaaacct cctagggaaa
     3661 ccatgttcct cagagatttt tcatgcctag tatgatctag tcacatgaaa cgaattattg
     3721 ttaacgtaat caattttata tagacagtta tgttttgtca attctttttt tataattata
     3781 gtaatttact ggaattatca gaaataaaag gaagaaataa attaagaaac ttatgttatt
     3841 taatcgttgg atcttttgct tatcttattc gttgttaaaa agctgaaagc gaaggaaaaa
     3901 aactctactt aactgtattt ctccgttagg acatctaaaa atttacgagc agcaggagaa
     3961 agaggctggt cttttttcca aatgagattc agtgaagctt gtaatggcgg atcgagtggg
     4021 atgaatattt tttccgtccc ataagtattg atgatcccat ctagagctaa tgcaccaaaa
     4081 gtcccgttat cgaccatcaa agaagcattg tacaataagt tatatgttcc gacgatcgat
     4141 aactgatcta tgctgaaacc tagccattca gctagttgac tgtccactga cgtttgccgg
     4201 gaaatgatca aaggaacagt tttcagttcc tgcggatgga tgacggtttg ctttgctaaa
     4261 ggatgatttc gatgtatcaa aagtccccaa cgatcttggg ctggcagtcg aaatgactgg
     4321 tatttttgtt tgtttgtcgg ttcgattacg agtccaaaat caagtaatcc gctatccagt
     4381 ttgtctatga tatcatctgc attcccgcta tataaatgaa actggatatc aggatgcgcc
     4441 ttgatcaacg aatgaaggct atcagaaatg aaagagattg ctttagtttc gcctccgcca
     4501 atgtagattt ctccagagat gattgcgtca tttgttaaat tgttcgccgt tttttctacc
     4561 aacgacaaga tttcttttcc ttttgaataa agatatcgac cagaatccgt caactgtatc
     4621 tctcgggagc cccgagtgaa aagtgtcaca ccaagttctt cttctaaatc tttcagttgt
     4681 tttgagagag taggttgcgt gatatgcaga gattttgctg ctcggctgat atttttttct
     4741 ctagctactg ctaaaaaata cgtcagtata cgaatatcca ttttttccct cctgttttta
     4801 aaaaagcata ccactaagta tagttaaaag gaatagttaa gtatgtttta taagtattag
     4861 acatgataag ccgtccgtta taaagtaagg ataaaaggag ggagcaaaat gacaagcctc
     4921 atcgaaaaac aaatcgtcaa taaagagatt cgagtaacgg atccgctctt tgaagtgatc
     4981 catcagatcc aagctgaaaa cgaagagcct ttagcagcgc tgaatactgg gtaccatgaa
     5041 ccagcagata tccgcaaacg gctagaaaat atcattagtg acaaagtaga tgatacggtg
     5101 actgttctgc tgccttttta tacagatttc ggcaaacata ttagtatagg gaaaaatgtc
     5161 tttatcaatc gtcaagtgat gttcgttgac ttaggtggca tctgtttaga agattcagta
     5221 ttgattggtc ctcgtgtcaa cctcatcacg gtgaaccatc taaccgatcc aaaagaacga
     5281 agaggcttgt cagtaaagcc aatccacatc aagaaaaatg cttggatcgg agcaggagca
     5341 actattctgc caggcgtaac gattggcgag aatgccattg ttgcagcgaa tgcaactgtt
     5401 acgaaagatg tcccagacaa taccatcgtt gcaggaatcc ctgccaaaat cgtcaaacct
     5461 gtcgaacgga ttatttgaac agtaagatga aataagagat agtgagtata agcaagagat
     5521 ttttcaaaac aggaggagaa tagaatgaat gatttatcag aaaaagtgat tgtcatcatg
     5581 ggcgcttcaa gcggaatcgg tgaagcaaca acgaagctat tagcagaaaa aggggctaaa
     5641 ctagttatag ctgcacgccg agaagatcgc ctaaaagcaa taaaagaatc tttaccagaa
     5701 gctgagctat atattcaaac agcagatgta agagattttg cacaagtcca agcagttatc
     5761 gatttagcga tggaaaagtt cggtcgaatc gatgtattgt acaataatgc aggaatcatg
     5821 ccaaccgctc cattagttga aggtcaccgt gatgaatggc aaaatatgct tgatatcaac
     5881 atcatgggtg tgttaaatgg aatttccgcc gtacttccaa tcatggaaaa acaaaaatct
     5941 gggcatatca tttcaactga ttctgtggcg ggacatgttg tttatcctga ttccgccgtt
     6001 tattgcggaa caaaatttgc tgtccgtgca atcatggaag gattacgtca agagcaacgg
     6061 caaaataaca tcaaatctac gatcatctct ccaggagctg ttcaaactga attatatcaa
     6121 accatcagcg acaaaaaggc tgctcttgaa cttcatgaag cgcaaaaaga atggggattg
     6181 acctccgaag atatagcttc tgctgtagca tttgcgatag aaacgccaga ccggatgtct
     6241 gtcagtaaca tgattatccg tccgacgaca caggaagtat aatccataac ggataatatc
     6301 caaagagaat tacttgttaa atagttattc aaacgaccat caatttaagg cgtgaatgga
     6361 gaagaatatt cttcatttac gtctttttat gtagaaaaag caaatttatc aaaaagaaac
     6421 aaatgttatt taaatatgga cattgacttg cttttacttt tttagttaca ctgttattta
     6481 ggaaaagttt cataattcaa aggagaaaaa agatgaaagc aaagaattta ttatttagcg
     6541 ttattctttt aggaaatcta gcagttgcca acacaacagt gctagctgaa gaatcgacag
     6601 aaactgtcgc aactcagaca tcgagttctt cagaaacagg aattgaaaac gatacagaga
     6661 gtactttgca gtcagctact gtctcagaaa atacaatttc aaccgaagaa ccaacaactg
     6721 aaacaacaga acaaacacaa accgaagaca gtacatcagc agaagctcag aacaatccag
     6781 agaatgcaga agaaatcacg aaccaattga cagatgggca aacattgaca catacccatc
     6841 aagcagattt gtctgaattc gaaaactata ctggctatgc tttttatgct cgctcatctg
     6901 ctaattctca acaagcgttc attgatagta ttgcctcaac tgcacaaagt ttggcaagtg
     6961 ccaatgactt atatgcatca gtcatgattg ctcaagccat cgttgaaagt ggctggggga
     7021 acagtacttt ggcttctgcc cctaattaca atctatttgg gattaaagga agctataatg
     7081 gccaatctgt tacgatgcct acttcggaat atgtgaatgg gcaatggatt acggtgaatg
     7141 ccgcttttag aaaatatcct tcgtacaaag aatcattgca agataacgta acggtattga
     7201 agacgacttc ttttcagcca ggcgtttact actattccgg tgcatggaaa agcaatacga
     7261 actcttacaa agatgctacg gcttggctaa caggtcgtta tgccactgcg cctaattatg
     7321 gttctacatt gaacaatgta atcgaaacct ataatctgac acagtatgat acagcaccaa
     7381 cttcaacttc tccgatgtat cgtctgtata accgtcatac aggagaacat ttatatacat
     7441 tgaatgccgg agaaaaaaat tatctgccaa ctgtaggctg ggaatatgaa gggattgctt
     7501 ggcaagcacc aaacagcgga caacctgtct accgtttata caatccttac agtggagacc
     7561 atcattacac aatggcacaa tctgagatca actctttaac aaaaatcggt tggcgttatg
     7621 aaggtctaag tttttattct ggtggttcca agccaattta tcgactattt aatccaaatg
     7681 aaaaaactgg tacccatcac tataccttga gtgctaagga aagagattgg ctaacaccga
     7741 tgggctggag atatgaagga ataggatttt atggttatta aaaagaggta gtgaaaaagc
     7801 tgccctgcat caagtagtaa gaacagccaa acaaaaacag attttcatgt tttttgtttg
     7861 gttgaactta ttaccgcaga tgcaagtttt tgaacaccgt ttactcaaaa aaggttgtga
     7921 caacatcttg tcataacttt tttattactg tttaagcata ctgatccaat ttctaatatt
     7981 cgacgggatt cttgcaaaag gcgatttcgg aaatttttgg cggtgtaaaa ttttctactg
     8041 cagaagagct atcttctata gtttgataag aatcaataat cttggtttta gcgttgtgct
     8101 ttctttatca ttttttccgt tcattctaca aaaaatttct tttctctata cttttctatt
     8161 gagagcgttt tttttattac acagctgatt ctttgacttt tttctttttt tattctatta
     8221 ttgacaagtc aatcgttgac gagtcaatta ttagaaagga ggaaattatg tctttagaag
     8281 agattagtca ccttttatat caaatcaaat tagccgatca aaaaatcgtc cagatctttg
     8341 aagaaaatat cggtataagt cttactagat acgagatcat gatgagactg aaagaagaaa
     8401 atccattgct acaaagcagt ttgcaagaaa agttgcagat tgatcaagga gcagttactc
     8461 gtcatctgaa ggtattggaa gcaaatggct atgtttcgcg tcagagaaat cctcagaaca
     8521 accgggaagt gtatgtagcg ctaacagaaa aagcccagaa gaaaatcgag cattgtgaaa
     8581 tggaccataa agatacaggt tccgtgctat ccccggcttt cagcgatgta gaaatgaaac
     8641 aatttctaac acttttagat aaattgaata cccaactttg caatataaca acagaaacga
     8701 aagaggttaa taataatgga acaaaaaaat gatttttcaa caattatgac tggtagaaga
     8761 tccgttcgcg tatatgatga aacatacaaa atcccacacg aagaaatgct ggaaatgatc
     8821 aaagaagcga cgactgcacc ttcttctgtc aatatgcaac catggcgctt tgtggtcgct
     8881 gaaagcgagg aagcaaaaga aatcttgcgt cctttgatcc gtttcaatac gagacaaaat
     8941 gatacttctt ctgcaatggt catgattttt ggcgatatgc aatgttacga atatggcgaa
     9001 gaaatctata accaagctta cgaatcaggg aagatgccaa aagaagtcag agatcaacaa
     9061 ttagctgcga tcattcctta ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc
     9121 aaagtcgatt ctagcttagc ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat
     9181 gaaacaaatc caattggtgg attcgaagca gatcagttgg cagaggcatt tggtttggat
     9241 aaagaccgtt atgttccagt tattatttta tcaataggaa aagcggtaga agaaggttac
     9301 gagtctgttc gtttagcacc agaaaagatc actacgttcg aataaaaggt tttcagttac
     9361 tattcagtag ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg
     9421 gaaaaatgac tgcatcgatg aaattacgtt tcgaactttt gaataacaac taaataaatt
     9481 caaaagacat gtataaacaa caatttcatg gatggagtat gtccattttt tcgaacgaca
     9541 gtagaatagg tgaaagctat gacagatttg gtgatttagt catagttatt tcgttcagag
     9601 taggaaaaga tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg
     9661 ataattgttg ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata
     9721 taacaaatct acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg
     9781 tctatcagca agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc
     9841 tcaaactaat agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct
     9901 tcaactacct gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag
     9961 ccagtcattt ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag
    10021 aacgaaagat cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg
    10081 agccaacaac ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt
    10141 attatggtac gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga
    10201 aaatctggga agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc
    10261 tcactcaaaa ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag
    10321 agaaaaaacg attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa
    10381 aagtgtccag taaaaagaaa aagcaacaaa tcagaccgga tcggttgtct tcctctaaac
    10441 aaaaagacag tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc
    10501 aaaaaatagg agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa
    10561 aatctcttga actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa
    10621 gtgggagaac attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga
    10681 ttgtcggtga aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag
    10741 aaggaatcct tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa
    10801 tgacatctga agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc
    10861 ttgtccgtag tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat
    10921 gtacattaag tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa
    10981 gtaatgtgtt gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt
    11041 tagagaagct aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact
    11101 ttgtcgagcg tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac
    11161 ttacgagaaa tttttaaatg tgctacgatg attttcgtga caaatttaaa ggagtggtac
    11221 agattgccta caaataaaaa agaaggaata atttttacaa caatgatgtg ttttttgatg
    11281 gtcttgggga tgagcgcgta taatttatgg ctgcatcaag atcttcattt atcaagtttg
    11341 ttgattggtc tagtgcctgg attcgtggtg gctttcattt tggatgtctt catcatcggt
    11401 gtgctggcga aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg
    11461 atcttaacta tttcatgctt gatggtttta ggaatggtga catgtatgtc tttattcggt
    11521 gtgatcatgg aacaaggaat acctgatcaa ttattctctg cttatatgaa tgcttggaaa
    11581 atgaatgtga ttgccgcttt accgttgcag ttgatcatcg ttggcccgct ttcaagaagt
    11641 gtgctaaaag caattcaaag taatgaatga tggaacactg ttatttgaaa aaaggccttg
    11701 gcatgctttt tgtcatagtc tcttgaatgc agttctagaa aaatagtgat ccgaacgata
    11761 ggttttggcc tgtcgttcgg atcgcttttt cttttccaac tgtattgctc ttttattgaa
    11821 gataatttgc tcttcaatag atatttctag aaaggttctg tttatccctt actcaaaaaa
    11881 ctgctgcatt tgttctctta cttgctgaca ccattggagt tcctgctgac aaagcataat
    11941 tctgcgctgg atcacaacat aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt
    12001 caattttttt tgattttttt gcatgatttt gttcagctgt tgttctctag tgaatagcag
    12061 tccagtagct gttgttttat cgagcgtcga aaaagcatag atttttgcta gaaattcatc
    12121 tttttgcgta ggagcgggag tttcctcttt gatccattcc tctacaagag cacgtccttc
    12181 atcagtcaag tggtagatct ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata
    12241 accctgctgg tctaacttgg caagcgttgt atagatctga ctgtgatggg cttcccaaaa
    12301 aattgtgatc agctgcttca attcataccc agaacgtggt ttggataaaa gcacgcataa
    12361 taatatatag gctaaagtat tcataaaaat cctacctctc gtcttatttt ctttattata
    12421 tcacagtaaa gtttccacgt tttacatgtg catatttaca tgtaaaataa atcatgtatg
    12481 atgctaatag agagaaaggg atggaaatat tggagaatca aatagatata caagaacaaa
    12541 caaatgaaga aacaaagggg aaaagttcag cgctagttgt agcagctacc tgtatgatcg
    12601 cttttatggg agtgggtcta gtagacccaa tcttaaaaac gattgctgca cagttaaacg
    12661 ctacgccagc tgaaacgacc ctgttattta ctagttacat gttagtcact ggtgtggtga
    12721 tgctatttac aggtttctta tctagtcgga tcggtttgaa aaaacattga tgacaggatt
    12781 gtttatcatc gttgtattcg ctggtcttgg cggattatct gggaatatcg gaatgctgat
    12841 tgggttacgt gccggctggg gtgtcgggaa tgctttattt gtctctactg ctttagcaac
    12901 gatcgtcagt attttagttg gtaacacaga aaaagcaatc atgatgtatg aagcagcact
    12961 tggctgcgga atggctgttg gccctctagt cggtggggtc ttgggcagtg gttcatggcg
    13021 ttatccattt tttggggtag catttttgat gttttgcggt tttatagccc tagcggtact
    13081 tttacctgaa actgagaaac caaagagaaa agtaggcctt tgggaaggtg tcaaagcgtt
    13141 aagcaatcat aaacttcgtt cagttggact aatcgcattg ttatataatt tcggattctt
    13201 tactttactt gcgtatgcgc catttttatt gattggctat tctgaattag aagtcggttt
    13261 tgtatttttt ggctggggtg tgcttctggc tattgcttcg attttcgttg caccggctct
    13321 tgagcggaag tttacgactt atcggacaat tttagcagca tttgtcttat tcattatttg
    13381 tttgctgtta ttaggagctg gaacttctat gccagtcctt gtaccgatca gcattgtgtt
    13441 agcaggattt ttccaaggaa ttatcaatac actgttgacg acgattgcta tggaaatacc
    13501 aggtctgcaa cgaaacgttg cttcgtccag ctatagtttt gtccgctttt tcggtggagc
    13561 tttagcacca tttattgcag gtgtgattgg tgaacatatt ggtgaaagct atgcgtttta
    13621 ttttgcaggt gccattgttc ttatcagtgt atgttgtatc atttatcacc gtaattattt
    13681 tgctacaaat gaagaagaat aagaaatcag aggttgtgaa aaagctgtcc tgcatcaagt
    13741 cataagaa
//
LOCUS       DAZKCZ010000304         8318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.guided.099, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000304 DAZKCZ010000000
VERSION     DAZKCZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.guided.099"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..1256
                     /locus_tag="ACG8KN_002759"
                     /pseudo
     CDS             129..1256
                     /locus_tag="ACG8KN_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            1354..1542
                     /locus_tag="ACG8KN_002760"
     CDS             1354..1542
                     /locus_tag="ACG8KN_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP9189706.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1600..1953)
                     /locus_tag="ACG8KN_002761"
     CDS             complement(1600..1953)
                     /locus_tag="ACG8KN_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189707.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2484..2906
                     /locus_tag="ACG8KN_002762"
     CDS             2484..2906
                     /locus_tag="ACG8KN_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9189708.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2903..3133
                     /locus_tag="ACG8KN_002763"
     CDS             2903..3133
                     /locus_tag="ACG8KN_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9189709.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3631..3831
                     /locus_tag="ACG8KN_002764"
     CDS             3631..3831
                     /locus_tag="ACG8KN_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFP9189710.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3858..5051
                     /locus_tag="ACG8KN_002765"
     CDS             3858..5051
                     /locus_tag="ACG8KN_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9189711.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5119..6684)
                     /gene="guaA"
                     /locus_tag="ACG8KN_002766"
     CDS             complement(5119..6684)
                     /gene="guaA"
                     /locus_tag="ACG8KN_002766"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9189712.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6743..7666)
                     /gene="coaA"
                     /locus_tag="ACG8KN_002767"
     CDS             complement(6743..7666)
                     /gene="coaA"
                     /locus_tag="ACG8KN_002767"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9189713.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7862..>8318
                     /locus_tag="ACG8KN_002768"
     CDS             7862..>8318
                     /locus_tag="ACG8KN_002768"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008376416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9189714.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYP"
BASE COUNT         2698 a         1507 c         1554 g         2559 t
ORIGIN      
        1 tcaattatca ttttcaattg cattttataa atcattttgt atgtaatttg gtatgtaagt
       61 taaaatgaaa aaagtagtcc attttgaagt acattttgtc attttgtact ccaaaactga
      121 actacttact tgcatatata cgcctttata gtggagtact acatttacga gattcggtta
      181 gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat
      241 tatgtaagat tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga
      301 agttaaatag tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc
      361 cgcaccctct actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc
      421 ttgatgccct tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta
      481 cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac
      541 tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg
      601 agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct
      661 gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga
      721 gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac
      781 gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa atctgtttta
      841 agtacggttt atactatagc cctgttagta ctccagcaga ttttcggatg cttactccta
      901 ttgtactgga gcaagccttt agaaaagctg gaacagaatt gttagagcca tatcttagtt
      961 ttaaagttta tgcaccacag gaatatcttt cacgggcata taacgatgct cccaaatatt
     1021 gtgcaaatat cgtaaatact caactgaaaa ataatgaggt cattattatt ggagaaattc
     1081 ctgctcgatg tattcaagat tatcgcaatg atttaacttt ttttacaaat gggcttagtg
     1141 tttgtttagc agagctaaaa ggatatcagg ttaccactgg cgaacctgtt tgccagaccc
     1201 gtcgtctaaa tagtcggata gataaagtaa gatatatgtt caataaaata acttagtgcg
     1261 ttttatgttg ttatataaat atggtttctt attaaataag atgaaatatt ctttaatata
     1321 gatttgaatt aaagtggaaa ggaggagatt gttattataa actacaagtg gatattgtgt
     1381 cctatttgtg gaaataaaac aagactacga atacgagtgg atactatact taaaaatttc
     1441 cctttatact gccccaaatg taagaacgaa actttaatta atgttcaaaa aatgaatata
     1501 ataacaatca aagagccaga cgccaagacg cagagccgat aatttgagaa atgaaactct
     1561 catcttatcg gctctttttg tttatctgaa ttttactgac tagccttcaa tatttcttgc
     1621 ttcattgata ccttttgcta gtgcttccat taaggatagt tctttgtctg taaagctatc
     1681 catgtatttt tctacctgta accttcgagt gcttttaatc aaattatctg taggtaagaa
     1741 aaattcatca atagatacat ggagtaatga tacaagatca tacagcactt gtgtacttgg
     1801 gtgttgtcct ttgttttcta tatttgttaa ataacgtggg tcaatttcaa tcattgttcc
     1861 cacttgttca cgagtgagac cccgtttcat tcgagcctct ttaatcgcta aaccaacggc
     1921 tctaaaatcg tatttatctt cttttttacg catactaaat cacctctata cattttactg
     1981 ttcctatata accataaaca ggtatagaaa aacatgttat atagtttatg agttcctata
     2041 gaacactgtg gaatttatta atgcgatgaa tccaatttca aatagaaagg tcgctgattt
     2101 tatttaccta tacaacttta taaaatttag tcatactcct agaaagtaga taaaattggc
     2161 gacttaatgg agggaaaagt cgccatgaga aagataatct gtcatctagt tctcttaatt
     2221 aagttcttat caaaaaaact tatccacctg tactggatag atatgaccat caatgtggtt
     2281 ttgcagatta catagataga aaaatcaaag catatgaaca gagaaaacaa tctgtttatg
     2341 tgcttttttg gtcacttaat aaatatcttt taggatttct cttcatttat gcaacaaatc
     2401 ccccgttcgt gtcggggttt agtatgaagg gagataaaga agcacatgcc actccctttc
     2461 acgacagaaa gggggtgaaa agcatgaaac catcttcttt tcaagaaacc atagaaaatc
     2521 agtttgacta tatctgcaaa caagttattg aagatgaacg aaaagattac ttcaaacatc
     2581 tagacagact atcagcaaaa gaagtctcgt tttctgatat agataactac cttgtcaact
     2641 ctttttctac tgttgatacg tacgtcactg atttccaact atttaaatta tttggattaa
     2701 gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg tcatctttca gagaaaaaac
     2761 ggaacattat tttacttcat tactttatgg atatgagtga tgtggagatt gcggagttac
     2821 taaatttgaa tcgttcgact gtttatcgtc atcgaataag tggactagcc atgattaaag
     2881 aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca agggttccct tccccctcat
     2941 tgtaaaggcg acagatggcg acgtggaagc aattaatcag attgttaaac attatcgagg
     3001 gtatacctca aaacgttcat tacgtcgcat gacagacgaa tatggaaatt ctcatatggt
     3061 tattgatgaa accctacgtg gcagaatgga aacgaaactc atctctaaga ttttatcgtt
     3121 cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga atcgtatcca cgcttcccga
     3181 tttgggaggt ttgttattcc atcaaagcat attgtgtgtt cagtaggttt tgataggcta
     3241 acaagccaaa cgttctttga caaccaaata aaggcaatcg aatacgtttc ggtaagaaaa
     3301 gagctgatga actaacacgc catgacctac aaaattagtg agcgattcat gttagtaatt
     3361 cgaaagtaat ctttagcagg attgctgcca tgactttctt atcgtgataa tgatactccc
     3421 gtatagccat agtccgagcg ttagaagcgt cacaggcaat gggtacggct acatgagaac
     3481 catgcagaag tgaaattcct gtgagctttg ctagagctgt tcgattgcta agatattctt
     3541 ctcttgtcag tgtattatta taaaactatt tgatataatt ataggtgaat attggagttt
     3601 tcctagccta taattgggaa aggagtaata atgataacgg tcacaaaaaa ggatttagtt
     3661 gagctaggat atggaacttc tttttcagca gacatcataa aaaaagcaaa agaattgatg
     3721 attacaaaag ggcatatcta ctatcaatct cgaaagctag atagagtccc tcgtgaagct
     3781 gttgaagaaa ttctaggaat tcggttttca aatgaaaaca ggtctgagta gtttttgcac
     3841 agtaaggagt aaaaactatg gttaaagacc caataaaaaa agcaaagaat ggaacctatt
     3901 attttcgtgc aaatctaggt tatgattcta aagggaaaaa aattcaaaaa tatcgtagtg
     3961 gattcactac taaaaaagaa gcaagagaag aatattcgaa actgctattg atgaaaccag
     4021 aagaattaag tgagaataag gataaaatga cgtttaaaca ttatatcttt gaaattttcc
     4081 ttccatggta taagacacag gtaaagctca gaacatatga aaatcgctta ccaactatta
     4141 agaagcattt ctcttatttt gataaaatgg ctgtttccga tattgagcct attgatgtgc
     4201 aaaattggca actaaaatta tcaaagaagt gtaagtcttc ttatgtaaga gctgttcaag
     4261 gactattttc agttgctatg gatcgagcaa ttgtccttgg gataaccacc acaaatccat
     4321 ctaagattat tggcaatgtg aaaaagcaaa agtctaaaat tgaattttgg acaaaagaag
     4381 aatttgaaaa agtaatctct ctaatttaca aagaagatta ctatcaacac tttttattta
     4441 tctctctgtg gtttttattt atgactggaa tgcgaattgg tgaagctact gccattcaat
     4501 gggaagatat tgattttgac tcaggagtac taaccatcaa caaaacactc tattacaaga
     4561 atcaatcgaa ttatagattt gttgaaccaa aaacaaaggc gagcgttcgt catattgtat
     4621 tagataaatg cacattaact tatttatccg aatggaagga agtacaacaa aatctaattc
     4681 agacagactt tgtaatgagc tataacggta ttcctaccca aaaacataca ctatcatatg
     4741 ctattgaacg ctatgctaag ctggctggta tccatcgaat cagaatccat gggttacggc
     4801 attctcatgc ttccttgtta atcagtatgg gagaaaatcc acttatcatt aaagatcgtc
     4861 taggacacga agatattgag acgactttag gaacgtatgg acatctttat cctaacagta
     4921 atttcgaggt agctaacaaa ttaaatggag ccatttcctt taaagaagca aaacaaaatt
     4981 atgatacttc accaaagaat caatttactg tagactactt gcgaaacaaa gaaatgaaaa
     5041 aagtgcaata aaagtgcagt aaaaagataa gaagagccag aaacccttgg tataagagga
     5101 tttctagctc tcctacaact attcccactc cactgttgct ggtggtttag aagtgatgtc
     5161 atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa
     5221 gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg
     5281 gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc
     5341 tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc
     5401 acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag
     5461 aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat
     5521 gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat
     5581 caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg
     5641 tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga
     5701 gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt
     5761 cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg
     5821 atctcttgca tcgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac
     5881 ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc
     5941 accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc
     6001 aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa
     6061 gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa
     6121 ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg
     6181 tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc
     6241 ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt
     6301 ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg
     6361 gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca
     6421 aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc
     6481 atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac
     6541 ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg
     6601 tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt
     6661 cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa
     6721 atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat
     6781 tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta
     6841 aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat
     6901 ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg
     6961 caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag
     7021 aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga
     7081 tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat
     7141 catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt
     7201 tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga
     7261 ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa
     7321 cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt
     7381 ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat
     7441 gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa agataaatca
     7501 gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac
     7561 ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc
     7621 gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg
     7681 ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga
     7741 gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact
     7801 tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga
     7861 catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc
     7921 ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga
     7981 aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga
     8041 tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt
     8101 tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca
     8161 aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga
     8221 agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa
     8281 aaaagttgta catgaaattt tgacaggcgc atatcctc
//
LOCUS       DAZKCZ010000305         8182 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.guided.100, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000305 DAZKCZ010000000
VERSION     DAZKCZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.guided.100"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG8KN_002769"
     CDS             complement(134..577)
                     /locus_tag="ACG8KN_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP9189715.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG8KN_002770"
     CDS             complement(795..2135)
                     /locus_tag="ACG8KN_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9189716.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG8KN_002771"
     CDS             2404..3300
                     /locus_tag="ACG8KN_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9189717.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG8KN_002772"
     CDS             complement(3450..4391)
                     /locus_tag="ACG8KN_002772"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9189718.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG8KN_002773"
     CDS             4517..5638
                     /locus_tag="ACG8KN_002773"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9189719.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8KN_002774"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8KN_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9189720.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG8KN_002775"
     CDS             7465..7992
                     /locus_tag="ACG8KN_002775"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9189721.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2719 a         1516 c         1611 g         2336 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg ga
//
LOCUS       DAZKCZ010000306         3192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.guided.157, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000306 DAZKCZ010000000
VERSION     DAZKCZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.guided.157"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..310
                     /locus_tag="ACG8KN_002776"
     CDS             <1..310
                     /locus_tag="ACG8KN_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9189722.1"
                     /translation="YLIVDLKLCNRTTAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFY
                     RLDNARSSDTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS
                     "
     gene            525..1493
                     /locus_tag="ACG8KN_002777"
     CDS             525..1493
                     /locus_tag="ACG8KN_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9189723.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1486..2517
                     /gene="vanA"
                     /locus_tag="ACG8KN_002778"
     CDS             1486..2517
                     /gene="vanA"
                     /locus_tag="ACG8KN_002778"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9189724.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2523..3131
                     /locus_tag="ACG8KN_002779"
     CDS             2523..3131
                     /locus_tag="ACG8KN_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP9189725.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          947 a          608 c          783 g          854 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccacc gcgggcctct ccggggatgt
       61 ggtgtcaatc gaattcaaga acactggaag catcccaaaa gataagctag ctgccatatt
      121 tgaaaagttc tataggctgg acaatgctcg ttcttccgat acgggtggcg cgggacttgg
      181 attggcgatt gcaaaagaaa ttattgttca gcatggaggg cagatttacg cggaaagcaa
      241 tgataactat acgacgttta gggtagagct tccagcgatg ccagacttgg ttgataaaag
      301 gaggtcctaa gagatgtata taatttttta ggaaaatctc aaggttatct ttactttttc
      361 ttaggaaatt aacaatttaa tattaagaaa cggctcgttc ttacacggta gacttaatac
      421 cgtaagaacg agccgttttc gttcttcaga gaaagatttg acaagattac cattggcatc
      481 cccgttttat ttggtgcctt tcacagaaag ggttggtctt aattatgaat aacatcggca
      541 ttactgttta tggatgtgag caggatgagg cagatgcatt ccatgctctt tcgcctcgct
      601 ttggcgttat ggcaacgata attaacgcca acgtgtcgga atccaacgcc aaatccgcgc
      661 ctttcaatca atgtatcagt gtgggacata aatcagagat ttccgcctct attcttcttg
      721 cgctgaagag agccggtgtg aaatatattt ctacccgaag catcggctgc aatcatatag
      781 atacaactgc tgctaagaga atgggcatca ctgtcgacaa tgtggcgtac tcgccggata
      841 gcgttgccga ttatactatg atgctaattc ttatggcagt acgcaacgta aaatcgattg
      901 tgcgctctgt ggaaaaacat gatttcaggt tggacagcga ccgtggcaag gtactcagcg
      961 acatgacagt tggtgtggtg ggaacgggcc agataggcaa agcggttatt gagcggctgc
     1021 gaggatttgg atgtaaagtg ttggcttata gtcgcagccg aagtatagag gtaaactatg
     1081 taccgtttga tgagttgctg caaaatagcg atatcgttac gcttcatgtg ccgctcaata
     1141 cggatacgca ctatattatc agccacgaac aaatacagag aatgaagcaa ggagcatttc
     1201 ttatcaatac tgggcgcggt ccacttgtag atacctatga gttggttaaa gcattagaaa
     1261 acgggaaact gggcggtgcc gcattggatg tattggaagg agaggaagag tttttctact
     1321 ctgattgcac ccaaaaacca attgataatc aatttttact taaacttcaa agaatgccta
     1381 acgtgataat cacaccgcat acggcctatt ataccgagca agcgttgcgt gataccgttg
     1441 aaaaaaccat taaaaactgt ttggattttg aaaggagaca ggagcatgaa tagaataaaa
     1501 gttgcaatac tgtttggggg ttgctcagag gagcatgacg tatcggtaaa atctgcaata
     1561 gagatagccg ctaacattaa taaagaaaaa tacgagccgt tatacattgg aattacgaaa
     1621 tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat gggaaaacga caattgctat
     1681 tcagctgtac tctcgccgga taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa
     1741 tatgaaatca accatgttga tgtagcattt tcagctttgc atggcaagtc aggtgaagat
     1801 ggatccatac aaggtctgtt tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa
     1861 agctcagcaa tttgtatgga caaatcgttg acatacatcg ttgcgaaaaa tgctgggata
     1921 gctactcccg ccttttgggt tattaataaa gatgataggc cggtggcagc tacgtttacc
     1981 tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat
     2041 agcgcggacg aattggacta cgcaattgaa tcggcaagac aatatgacag caaaatctta
     2101 attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg
     2161 ttagttgttg gcgaggtgga ccaaatcagg ctgcagtacg gaatctttcg tattcatcag
     2221 gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa ccgttcccgc agacctttca
     2281 gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt
     2341 agaggtctag cccgtgtgga tatgttttta caagataacg gccgcattgt actgaacgaa
     2401 gtcaatactc tgcccggttt cacgtcatac agtcgttatc cccgtatgat ggccgctgca
     2461 ggtattgcac ttcccgaact gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa
     2521 gcatggaaat aggatttact tttttagatg aaatagtaca cggtgttcgt tgggacgcta
     2581 aatatgccac ttgggataat ttcaccggaa aaccggttga cggttatgaa gtaaatcgca
     2641 ttgtagggac atacgagttg gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc
     2701 aagggtacgg attgcttcta tgggacggtt accgtcctaa gcgtgctgta aactgtttta
     2761 tgcaatgggc tgcacagccg gaaaataacc tgacaaagga aagttattat cccaatattg
     2821 accgaactga gatgatttca aaaggatacg tggcttcaaa atcaagccat agccgcggca
     2881 gtgccattga tcttacgctt tatcgattag acacgggtga gcttgtacca atggggagcc
     2941 gatttgattt tatggatgaa cgctctcatc atgcggcaaa tggaatatca tgcaatgaag
     3001 cgcaaaatcg cagacgtttg cgctccatca tggaaaacag tgggtttgaa gcatatagcc
     3061 tcgaatggtg gcactatgta ttaagagacg aaccataccc caatagctat tttgatttcc
     3121 ccgttaaata aacttttaac cgttgcacgg acaaacggtt ctgttgcaaa gttttaaata
     3181 aagaataaaa tc
//
LOCUS       DAZKCZ010000307         2265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.guided.181, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000307 DAZKCZ010000000
VERSION     DAZKCZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.guided.181"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..283)
                     /locus_tag="ACG8KN_002780"
     CDS             complement(<1..283)
                     /locus_tag="ACG8KN_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFP9189726.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIV"
     gene            376..726
                     /locus_tag="ACG8KN_002781"
     CDS             376..726
                     /locus_tag="ACG8KN_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9189727.1"
                     /translation="MLNTLFDAKEIYDSQQKKFSLYPEKFFLVKDLWIAVMQNSSNKL
                     PKTYNHIAFKIDEQDIDSFVSKIQMLGLTVEPGRSRVKDEASSIYFYDYDNHLFELHT
                     GTLQERLKSYNSTT"
     gene            complement(998..1288)
                     /locus_tag="ACG8KN_002782"
     CDS             complement(998..1288)
                     /locus_tag="ACG8KN_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9189728.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1496..2188)
                     /locus_tag="ACG8KN_002783"
                     /pseudo
     CDS             complement(1496..2188)
                     /locus_tag="ACG8KN_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          648 a          430 c          329 g          858 t
ORIGIN      
        1 ctacaattcc taacataatc gtagaattta ataatttcca tagcataaag tgtttagaaa
       61 agaacattaa gaatagttct ataataattc ttgaactcca tgtcttataa tgttctatcg
      121 aagcacccaa atagccaagt tcatctaatc tacttgcata aactacgtca tccgctctca
      181 tagtcggcat atacatatta aagagtaaaa agatacccca aatccctatt agtaacaaga
      241 tcagttcttt gttattcgtt atttttctat tatgtttgtt catttttata ctccttctta
      301 tatttcctaa tagagtataa catagcttta agcattactt aaagataact taaaagttat
      361 cttttaaata cttaattgtt aaatacctta tttgatgcaa aagaaatcta cgatagtcaa
      421 caaaaaaaat tttcattata tccagaaaaa tttttcttag ttaaggacct atggattgca
      481 gtaatgcaga attcgtcgaa taaactgcca aagacataca atcatattgc ttttaaaatt
      541 gacgaacaag atatagattc gtttgtctca aaaatccaaa tgttaggttt aactgtcgaa
      601 ccaggacgtt ctcgtgtgaa agacgaggct tcttcaattt acttttacga ctatgataat
      661 cacctcttcg agttacatac cggtactctc caggaacgtt taaaatccta caattccact
      721 acctgagact agtaatgact agtctctttt tatggcctct gatcccccca ttgagcgaat
      781 actaataggg cttcgaattg atctgttcaa tctttaagca atatccctca aatgagtttg
      841 gaaagtttag tgattcatca caaatactta aatacatgta gttatatata gattgaggtt
      901 cttgtgagcg tatgctcgtg tgggttcaaa tcccatcgtc tgaatccatc tttccaaaac
      961 aagcggctag gacatttttg tctcagccgc ttgttttcta gtttattctt tcatcttgtc
     1021 ggaccttttt tcttgggtat agacttttct catattctac cacaattttg ggtttcccta
     1081 acacatgata ggatctcaca acaaattgct ttctattatt ataataacct gtaacgacta
     1141 acttcatccc ttcgtctact tcagctagga agttcaaact atgggaagca atcaaacaat
     1201 tgaccccatt taaactgaaa cggatcaacg gtctggcact aacttgcaaa gtacggatct
     1261 tatctactgt tccatatttt ttctccataa ccttcacctc aataccatta tacgaacata
     1321 cattcccttt gtaaagaaga aactctgttt tatctttttg actttttgtc tattaccttt
     1381 cttaaaatca tattggcact tcctgcgaag atgaaggaca tgtactaaaa aaaagaggca
     1441 gcagtataga ttactgttgc ttctttacta atttaggttt acttgttaag aaagattatt
     1501 tacgcaaatg ttcttcaaca atttgctgag atttatcagc tgtatcttcc acttcatctt
     1561 ttgctttttc taacttttga atatattgtt tcaagcttga gttttcagca gttgcttcgt
     1621 ctaatttctt ttgtgtatct gttaactgag tttctacatt tgttttttct tgtgtcagtg
     1681 ttgcgatttg ttgattcaaa ccatttacag tattttgtaa ttctgaaagt tttgcttgtg
     1741 cttcttgtag tcttgctgtc caatctgcat tcagatgatt gatttcatca tttttttagt
     1801 tgatctcatc tagtttgaca ttgatttcat tttgcttgct ttgtagttgt gcatccttgc
     1861 tgactaattg actttctaag ttttgttttt ctgctgccga ttgatttttt tggttttcta
     1921 attcatttac ttttgtttgg tattcttgga catctgcctt tgaatcttgc aatgtctgtt
     1981 ctttttgtgc tagtcgagcg gtaagatctg taatcgcttg ctctttatct gtcaatgtat
     2041 gatcaagctg tgtcagattc tcattgattg tatcaatatc tgtctctcca ccccacaaaa
     2101 taatttcatt tgcgtaccgc gtgcctgtgt agccaattag acatagaaat gcaacgacaa
     2161 tcccaccagt aataaatttc tttttcattt gcatagctcc ttaatccaat acttttacat
     2221 actaaaagta agtgatctca aacacaatgt caagcgtttt taaat
//
LOCUS       DAZKCZ010000308         1255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.guided.229, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000308 DAZKCZ010000000
VERSION     DAZKCZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.guided.229"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..771
                     /gene="erm(B)"
                     /locus_tag="ACG8KN_002784"
     CDS             34..771
                     /gene="erm(B)"
                     /locus_tag="ACG8KN_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP9189729.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            941..1201
                     /locus_tag="ACG8KN_002785"
                     /pseudo
     CDS             941..1201
                     /locus_tag="ACG8KN_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          477 a          208 c          210 g          360 t
ORIGIN      
        1 gtttatagat gtaatcactt caggagagat tacatgaaca aaaatataaa atattctcaa
       61 aactttttaa cgagtgaaaa agtactcaac caaataataa aacaattgaa tttaaaagaa
      121 accgataccg tttacgaaat tggaacaggt aaagggcatt taacgacgaa actggctaaa
      181 ataagtaaac aggtaacgtc tattgaatta gacagtcatc tattcaactt atcgtcagaa
      241 aaattaaaac tgaatactcg tgtcacttta attcaccaag atattctaca gtttcaattc
      301 cctaacaaac agaggtataa aattgttggg aatattcctt accatttaag cacacaaatt
      361 attaaaaaag tggtttttga aagccgtgcg tctgacatct atctgattgt tgaagaagga
      421 ttctacaagc gtaccttgga tattcaccga acactagggt tgctcttgca cactcaagtc
      481 tcgattcagc aattgcttaa gctgccagcg gaatgctttc atcctaaacc aaaagtaaac
      541 agtgtcttaa taaaacttac ccgccatacc acagatgttc cagataaata ttggaagcta
      601 tatacgtact ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt tactaaaaat
      661 cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt taagtaccat tacttatgag
      721 caagtattgt ctatttttaa tagttatcta ttatttaacg ggaggaaata attctatgag
      781 tcgctttttt aaatttggaa agttacacgt tactaaaggg aatggagata aattattaga
      841 tatactactg acagcttcca agaagctaaa gaggtcccta gcgcctacgg ggaatttgta
      901 tcgataaggg gtacaaattc ccactaagcg ctcgggaccc cttgtaggaa aatgtcctaa
      961 gtgtggcaac aatattgtat taaaaaaatc gttttatggt tgttcaaatt atcctgaatg
     1021 taagtttact ttagctgaac attttagaaa gaaaaaacta accaaaacga atgtaaaaga
     1081 attactggag ggaaaagaaa ccctggtaaa aggaatcaaa aacaaagaga aaaagcccta
     1141 caatgccgtt gtaaaaattg gggaaaaggg atatattgat tttatatctt tctcaaaata
     1201 aacataaaag ccctttaaag agggctttta tatattaatc acaaatcact tatca
//
LOCUS       DAZKCZ010000309         1250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.guided.231, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000309 DAZKCZ010000000
VERSION     DAZKCZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.guided.231"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..164
                     /locus_tag="ACG8KN_002786"
     CDS             <1..164
                     /locus_tag="ACG8KN_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9189730.1"
                     /translation="RTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGMADEMRRPMM
                     LIVSAKKKM"
     gene            197..1105
                     /locus_tag="ACG8KN_002787"
     CDS             197..1105
                     /locus_tag="ACG8KN_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9189731.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
BASE COUNT          445 a          180 c          278 g          347 t
ORIGIN      
        1 atagaacact gaccacatat ctaaatacac tgctttcaaa tagttttata ataaatcaga
       61 ttgtggagcc acagccgcca gagaacatga tggatattcc ggggatggcg gatgaaatgc
      121 gacgcccaat gatgctgatt gtatcggcaa aaaagaagat gtaataatat agaaaaaata
      181 aacgaggagt atgtaaatga gatcagaaaa agaaatgatg gatttagtac tttctttagc
      241 agaacaggat gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa atattaatat
      301 acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata tagaaccgtt
      361 tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc aaaagccgga
      421 ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc tatttgatga
      481 ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt acctaaaggg
      541 cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg acatagttcc
      601 gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg attgctgcaa
      661 tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg aaattttatt
      721 tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga tatcatggaa
      781 ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt ttattgaaag
      841 gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg attcctatga
      901 aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat ccggtgaggt
      961 ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa aatataccag
     1021 ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat atgccgctga
     1081 tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct gaaagatatc
     1141 gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta tgaaaacgag
     1201 aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta
//
LOCUS       DAZKCZ010000310         1103 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.guided.239, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000310 DAZKCZ010000000
VERSION     DAZKCZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.guided.239"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..870
                     /gene="vanY"
                     /locus_tag="ACG8KN_002788"
     CDS             <1..870
                     /gene="vanY"
                     /locus_tag="ACG8KN_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFP9189732.1"
                     /translation="YLGYDYVNEALFSQEKVEFQNYDQNPKEHLENSGTSENTQEKTI
                     TEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDSNIYMSKEIAQKF
                     SEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEHNSGLSLDVGSS
                     LTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIRYVGLPHSAIMKEKN
                     FVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEISGNNIDGVIV
                     TVFPGSTHTNSRR"
BASE COUNT          406 a          155 c          221 g          321 t
ORIGIN      
        1 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
       61 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      121 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      181 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      241 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      301 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      361 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      421 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      481 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      541 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      601 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      661 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
      721 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
      781 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
      841 ggatcaacac atactaattc aaggaggtaa ggatggcgga atgaaaccaa cgaaattaat
      901 gaacagcatt attgtactag cacttttggg gtaacgttag ctttttaatt taaaacccac
      961 gttaactagg acattgctat actaatgata caacttaaac aaaagaatta gaggaaatta
     1021 tattgggaaa aatattatct agaggattgc tagctttata tttagtgaca ctaatctggt
     1081 tagtgttatt caaattacaa tac
//
LOCUS       DAZKCZ010000311          929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.guided.250, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000311 DAZKCZ010000000
VERSION     DAZKCZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.guided.250"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..379
                     /locus_tag="ACG8KN_002789"
     CDS             <1..379
                     /locus_tag="ACG8KN_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFP9189733.1"
                     /translation="DHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYISEDL
                     WEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRDMYK
                     KYTGKTGCLDSTYAADIEERREQ"
     gene            376..856
                     /locus_tag="ACG8KN_002790"
                     /pseudo
     CDS             376..856
                     /locus_tag="ACG8KN_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          318 a          145 c          214 g          252 t
ORIGIN      
        1 tgatcatttt aatcagattg ttcgccatga gctgctgaga atgatatcat ggaaggtcgg
       61 catcgaaaca ggctttaaat taagtgtagg caagaactat aagtttattg aaaggtatat
      121 atccgaggat ttgtgggaga aacttttgtc cacctaccgg atggattcct atgaaaacat
      181 atgggaagca ttatttctat gccatcaatt gttcagggcg gtatccggtg aggtggcgga
      241 aaggcttcat tatgcctatc cggagtatga taggaatata acaaaatata ccagggacat
      301 gtataaaaaa tacactggta aaaccggctg cctggatagc acatatgccg ctgatataga
      361 agagaggcgg gaacagtgat tacagaaatg aaagcagggc acctgaaaga tatcgataaa
      421 cccagcgaac catttgaggt gataggtaag attataccga ggtatgaaaa cgagaattgg
      481 acctttacag aattactcta tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat
      541 gaagaggatg aggaggcaga ttgccttgaa tatattgaca atactgataa gataatatat
      601 cttttatata gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc
      661 gcttatcaat atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga
      721 aacccaggac aataacctta tagcttgtaa attctatcat aattgtggtt tcaaaatcgg
      781 ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg
      841 gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt ggttctgttg
      901 caaagtttta aataaagaat aaaatccct
//
LOCUS       DAZKCZ010000312          852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.guided.256, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000312 DAZKCZ010000000
VERSION     DAZKCZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.guided.256"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            239..787
                     /gene="aac(6')"
                     /locus_tag="ACG8KN_002791"
     CDS             239..787
                     /gene="aac(6')"
                     /locus_tag="ACG8KN_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9189734.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          306 a          126 c          180 g          240 t
ORIGIN      
        1 ttttaaagca aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga
       61 tggctttcct gatctttcct attttgaata aagtgtttct gtataatttt tttattttat
      121 aagtaagata aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga
      181 gaacatttcg ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat
      241 gataatcagt gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact
      301 gagactgact tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat
      361 gaatccagaa cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg
      421 tgcaatccct caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc
      481 ccgacgaaag aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc
      541 cagaggagga atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag
      601 tcaaacggac ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga
      661 acatccgtat gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc
      721 aaatggctgg gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc
      781 tcaataataa aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta
      841 cgaaaattac tt
//
LOCUS       DAZKCZ010000313          844 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.guided.257, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000313 DAZKCZ010000000
VERSION     DAZKCZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.guided.257"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..391
                     /locus_tag="ACG8KN_002792"
     CDS             <1..391
                     /locus_tag="ACG8KN_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9189735.1"
                     /translation="FNYHQRSLNLTPFTATGNFREMIDNVIIFIPFGLLLNVNFKEIG
                     FLPKFAFVLVLSLTFEIIQFIFAIGATDITDVITNTVGGFLGLKLYGLSNKHMNQKKL
                     DRVIIFVGILLLVLLLVYRTHLRINYV"
BASE COUNT          281 a          129 c          147 g          287 t
ORIGIN      
        1 atttaattat catcaaagaa gtcttaactt gactccattt actgctactg ggaatttcag
       61 agagatgata gataatgtta taatctttat tccatttggc ttgcttttga atgtcaattt
      121 taaagaaatc ggatttttac ctaagtttgc ttttgtactg gttttaagtc ttacttttga
      181 aataattcaa tttatcttcg ctattggagc gacagacata acagatgtaa ttacaaatac
      241 tgttggaggc tttcttggac tgaaattata tggtttaagc aataagcata tgaatcaaaa
      301 aaaattagac agagttatta tttttgtagg tatacttttg ctcgtattat tgctcgttta
      361 ccgtacccat ttaagaataa attacgtgta agatgtctaa atcaagcaat ctgatctttc
      421 atacacataa agatattgaa tgaattggat tagatggaaa acgggatgtg gggaaactcg
      481 cccgtaggtg tgaagtgagg ggaaaaccgg tgataaagta aaaagcttac ctaacactat
      541 agtaacaaag aaagcccaat tatcaatttt agtgctgagg aattggtctc tttaataaat
      601 ttccttaacg ttgtaaatcc gcattttcct gacggtaccc ccactagcag ccatgctttt
      661 tttgaatctt tgtaggacgt tttaaagaga attacccact ttaaaattaa acatgcgctt
      721 agaatcgctt taggaaacac gatccagtcc aataatcgtc gataaaaact tttgaaaaag
      781 gttggtgaaa ttacctactt ttggaataat cacaaatcac aagtgattaa tcacttgttt
      841 atta
//
LOCUS       DAZKCZ010000314          677 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4112
            SAMEA115707014-rid22570193.guided.273, whole genome shotgun
            sequence.
ACCESSION   DAZKCZ010000314 DAZKCZ010000000
VERSION     DAZKCZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707014
            Sequence Read Archive: ERR13196746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287852.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:37:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,793
            CDSs (total)                      :: 2,730
            Genes (coding)                    :: 2,636
            CDSs (with protein)               :: 2,636
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 45 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 23 of 94
            Pseudo Genes (multiple problems)  :: 16 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707014-rid22570193.guided.273"
                     /isolate="VRE4112"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..677
                     /gene="dfrG"
                     /locus_tag="ACG8KN_002793"
     CDS             180..677
                     /gene="dfrG"
                     /locus_tag="ACG8KN_002793"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9189736.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          263 a           63 c          139 g          212 t
ORIGIN      
        1 gatttataaa atgcaattga aaatgataat tgaatgaaaa tttgtgattt tgtggtatta
       61 tgataatata acttatagaa aaaattgctt attcaactaa cgggcaggat aatgagaaaa
      121 ggttttttga tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa
      181 tgaaagtttc tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca
      241 ttccttggag gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga
      301 taatattagg taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata
      361 ttattctgac gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag
      421 aagatgtttt tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga
      481 tttataattt gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat
      541 tcgaaggaga tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc
      601 aaaaagggat aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa
      661 aaaacttatt gagttaa
//
